LOCUS       DAFAVF010000001        90610 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFAVF010000001 DAFAVF010000000
VERSION     DAFAVF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.001"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..285
                     /locus_tag="LGK42_000001"
     CDS             <1..285
                     /locus_tag="LGK42_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBK2656296.1"
                     /translation="VFLVIFYGIIILHDIPYLIAKKRNHPHADAIHVAGWVSLFTLHV
                     IWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            291..1427
                     /locus_tag="LGK42_000002"
     CDS             291..1427
                     /locus_tag="LGK42_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2656297.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1424..2398)
                     /locus_tag="LGK42_000003"
     CDS             complement(1424..2398)
                     /locus_tag="LGK42_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2656298.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            2522..3262
                     /locus_tag="LGK42_000004"
     CDS             2522..3262
                     /locus_tag="LGK42_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HBK2656299.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFVTGVSWRF"
     gene            complement(3383..5362)
                     /locus_tag="LGK42_000005"
     CDS             complement(3383..5362)
                     /locus_tag="LGK42_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBK2656300.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVISYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGV
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(5373..6773)
                     /locus_tag="LGK42_000006"
     CDS             complement(5373..6773)
                     /locus_tag="LGK42_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656301.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKITWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            6999..7814
                     /locus_tag="LGK42_000007"
     CDS             6999..7814
                     /locus_tag="LGK42_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2656302.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(7846..8541)
                     /gene="araD"
                     /locus_tag="LGK42_000008"
     CDS             complement(7846..8541)
                     /gene="araD"
                     /locus_tag="LGK42_000008"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK2656303.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(8535..9395)
                     /locus_tag="LGK42_000009"
     CDS             complement(8535..9395)
                     /locus_tag="LGK42_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK2656304.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(9388..10050)
                     /gene="ulaD"
                     /locus_tag="LGK42_000010"
     CDS             complement(9388..10050)
                     /gene="ulaD"
                     /locus_tag="LGK42_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK2656305.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVMQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(10047..11543)
                     /gene="lyxK"
                     /locus_tag="LGK42_000011"
     CDS             complement(10047..11543)
                     /gene="lyxK"
                     /locus_tag="LGK42_000011"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBK2656306.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(11547..12533)
                     /gene="yiaO"
                     /locus_tag="LGK42_000012"
     CDS             complement(11547..12533)
                     /gene="yiaO"
                     /locus_tag="LGK42_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBK2656307.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(12546..13823)
                     /gene="yiaN"
                     /locus_tag="LGK42_000013"
     CDS             complement(12546..13823)
                     /gene="yiaN"
                     /locus_tag="LGK42_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBK2656308.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(13826..14299)
                     /gene="yiaM"
                     /locus_tag="LGK42_000014"
     CDS             complement(13826..14299)
                     /gene="yiaM"
                     /locus_tag="LGK42_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBK2656309.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(14417..14884)
                     /locus_tag="LGK42_000015"
     CDS             complement(14417..14884)
                     /locus_tag="LGK42_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBK2656310.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(14896..15894)
                     /gene="yiaK"
                     /locus_tag="LGK42_000016"
     CDS             complement(14896..15894)
                     /gene="yiaK"
                     /locus_tag="LGK42_000016"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBK2656311.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            16095..16943
                     /gene="yiaJ"
                     /locus_tag="LGK42_000017"
     CDS             16095..16943
                     /gene="yiaJ"
                     /locus_tag="LGK42_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBK2656312.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            17045..17518
                     /locus_tag="LGK42_000018"
     CDS             17045..17518
                     /locus_tag="LGK42_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK2656313.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(17670..18923)
                     /gene="avtA"
                     /locus_tag="LGK42_000019"
     CDS             complement(17670..18923)
                     /gene="avtA"
                     /locus_tag="LGK42_000019"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBK2656314.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(19101..21131)
                     /gene="malS"
                     /locus_tag="LGK42_000020"
     CDS             complement(19101..21131)
                     /gene="malS"
                     /locus_tag="LGK42_000020"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBK2656315.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            21451..22275
                     /locus_tag="LGK42_000021"
     CDS             21451..22275
                     /locus_tag="LGK42_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBK2656316.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(22383..23561)
                     /gene="xylR"
                     /locus_tag="LGK42_000022"
     CDS             complement(22383..23561)
                     /gene="xylR"
                     /locus_tag="LGK42_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HBK2656317.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(23639..24820)
                     /gene="xylH"
                     /locus_tag="LGK42_000023"
     CDS             complement(23639..24820)
                     /gene="xylH"
                     /locus_tag="LGK42_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBK2656318.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(24798..26339)
                     /gene="xylG"
                     /locus_tag="LGK42_000024"
     CDS             complement(24798..26339)
                     /gene="xylG"
                     /locus_tag="LGK42_000024"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBK2656319.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(26417..27409)
                     /gene="xylF"
                     /locus_tag="LGK42_000025"
     CDS             complement(26417..27409)
                     /gene="xylF"
                     /locus_tag="LGK42_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2656320.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            27775..29097
                     /gene="xylA"
                     /locus_tag="LGK42_000026"
     CDS             27775..29097
                     /gene="xylA"
                     /locus_tag="LGK42_000026"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBK2656321.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            29169..30623
                     /gene="xylB"
                     /locus_tag="LGK42_000027"
     CDS             29169..30623
                     /gene="xylB"
                     /locus_tag="LGK42_000027"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBK2656322.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            30792..31133
                     /locus_tag="LGK42_000028"
     CDS             30792..31133
                     /locus_tag="LGK42_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656323.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            31179..31616
                     /locus_tag="LGK42_000029"
     CDS             31179..31616
                     /locus_tag="LGK42_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBK2656324.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(31658..32653)
                     /gene="wecH"
                     /locus_tag="LGK42_000030"
     CDS             complement(31658..32653)
                     /gene="wecH"
                     /locus_tag="LGK42_000030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBK2656325.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            32828..33127
                     /locus_tag="LGK42_000031"
     CDS             32828..33127
                     /locus_tag="LGK42_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBK2656326.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            33222..34133
                     /gene="glyQ"
                     /locus_tag="LGK42_000032"
     CDS             33222..34133
                     /gene="glyQ"
                     /locus_tag="LGK42_000032"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK2656327.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            34143..36212
                     /gene="glyS"
                     /locus_tag="LGK42_000033"
     CDS             34143..36212
                     /gene="glyS"
                     /locus_tag="LGK42_000033"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK2656328.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            36538..36690
                     /gene="hokA"
                     /locus_tag="LGK42_000034"
     CDS             36538..36690
                     /gene="hokA"
                     /locus_tag="LGK42_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBK2656329.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGCYELAAFL
                     ACNLKE"
     gene            complement(36878..37090)
                     /gene="cspA"
                     /locus_tag="LGK42_000035"
     CDS             complement(36878..37090)
                     /gene="cspA"
                     /locus_tag="LGK42_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBK2656330.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(37371..37661)
                     /locus_tag="LGK42_000036"
     CDS             complement(37371..37661)
                     /locus_tag="LGK42_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBK2656331.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            38095..38805
                     /locus_tag="LGK42_000037"
     CDS             38095..38805
                     /locus_tag="LGK42_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBK2656332.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(38855..39829)
                     /gene="ghrB"
                     /locus_tag="LGK42_000038"
     CDS             complement(38855..39829)
                     /gene="ghrB"
                     /locus_tag="LGK42_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBK2656333.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(39933..40592)
                     /locus_tag="LGK42_000039"
     CDS             complement(39933..40592)
                     /locus_tag="LGK42_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBK2656334.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            40745..43078
                     /gene="bisC"
                     /locus_tag="LGK42_000040"
     CDS             40745..43078
                     /gene="bisC"
                     /locus_tag="LGK42_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBK2656335.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(43047..43487)
                     /locus_tag="LGK42_000041"
     CDS             complement(43047..43487)
                     /locus_tag="LGK42_000041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2656336.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(43484..44047)
                     /gene="tag"
                     /locus_tag="LGK42_000042"
     CDS             complement(43484..44047)
                     /gene="tag"
                     /locus_tag="LGK42_000042"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK2656337.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            44205..44903
                     /locus_tag="LGK42_000043"
     CDS             44205..44903
                     /locus_tag="LGK42_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2656338.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            45132..46340
                     /locus_tag="LGK42_000044"
     CDS             45132..46340
                     /locus_tag="LGK42_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656339.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTRLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            46664..48355
                     /gene="eptB"
                     /locus_tag="LGK42_000045"
     CDS             46664..48355
                     /gene="eptB"
                     /locus_tag="LGK42_000045"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBK2656340.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            48447..48523
                     /locus_tag="LGK42_000046"
     tRNA            48447..48523
                     /locus_tag="LGK42_000046"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48481..48483,aa:Pro,seq:cgg)
     gene            48558..48691
                     /locus_tag="LGK42_000047"
     ncRNA           48558..48691
                     /ncRNA_class="other"
                     /locus_tag="LGK42_000047"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            49436..51043
                     /gene="dppA"
                     /locus_tag="LGK42_000048"
     CDS             49436..51043
                     /gene="dppA"
                     /locus_tag="LGK42_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBK2656341.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            51351..52370
                     /gene="dppB"
                     /locus_tag="LGK42_000049"
     CDS             51351..52370
                     /gene="dppB"
                     /locus_tag="LGK42_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBK2656342.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            52380..53282
                     /gene="dppC"
                     /locus_tag="LGK42_000050"
     CDS             52380..53282
                     /gene="dppC"
                     /locus_tag="LGK42_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBK2656343.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            53293..54276
                     /gene="dppD"
                     /locus_tag="LGK42_000051"
     CDS             53293..54276
                     /gene="dppD"
                     /locus_tag="LGK42_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBK2656344.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            54273..55277
                     /gene="dppF"
                     /locus_tag="LGK42_000052"
     CDS             54273..55277
                     /gene="dppF"
                     /locus_tag="LGK42_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBK2656345.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(55307..56578)
                     /locus_tag="LGK42_000053"
     CDS             complement(55307..56578)
                     /locus_tag="LGK42_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBK2656346.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            57054..57161
                     /locus_tag="LGK42_000054"
     CDS             57054..57161
                     /locus_tag="LGK42_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBK2656347.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(57248..58927)
                     /gene="bcsG"
                     /locus_tag="LGK42_000055"
     CDS             complement(57248..58927)
                     /gene="bcsG"
                     /locus_tag="LGK42_000055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBK2656348.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(58924..59115)
                     /gene="bcsF"
                     /locus_tag="LGK42_000056"
     CDS             complement(58924..59115)
                     /gene="bcsF"
                     /locus_tag="LGK42_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBK2656349.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(59112..60683)
                     /gene="bcsE"
                     /locus_tag="LGK42_000057"
     CDS             complement(59112..60683)
                     /gene="bcsE"
                     /locus_tag="LGK42_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBK2656350.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            60956..61144
                     /locus_tag="LGK42_000058"
     CDS             60956..61144
                     /locus_tag="LGK42_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBK2656351.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            61156..61889
                     /gene="bcsQ"
                     /locus_tag="LGK42_000059"
                     /pseudo
     CDS             61156..61889
                     /gene="bcsQ"
                     /locus_tag="LGK42_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            61886..64504
                     /gene="bcsA"
                     /locus_tag="LGK42_000060"
     CDS             61886..64504
                     /gene="bcsA"
                     /locus_tag="LGK42_000060"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBK2656352.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            64515..66854
                     /gene="bcsB"
                     /locus_tag="LGK42_000061"
     CDS             64515..66854
                     /gene="bcsB"
                     /locus_tag="LGK42_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBK2656353.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            66861..67967
                     /gene="bcsZ"
                     /locus_tag="LGK42_000062"
     CDS             66861..67967
                     /gene="bcsZ"
                     /locus_tag="LGK42_000062"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBK2656354.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            67949..71422
                     /gene="bcsC"
                     /locus_tag="LGK42_000063"
     CDS             67949..71422
                     /gene="bcsC"
                     /locus_tag="LGK42_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBK2656355.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGNSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDHDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWREHTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            71504..73492
                     /gene="hmsP"
                     /locus_tag="LGK42_000064"
     CDS             71504..73492
                     /gene="hmsP"
                     /locus_tag="LGK42_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBK2656356.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            73675..74961
                     /gene="dctA"
                     /locus_tag="LGK42_000065"
     CDS             73675..74961
                     /gene="dctA"
                     /locus_tag="LGK42_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBK2656357.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            75182..76678
                     /locus_tag="LGK42_000066"
     CDS             75182..76678
                     /locus_tag="LGK42_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK2656358.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(76774..77703)
                     /gene="kdgK"
                     /locus_tag="LGK42_000067"
     CDS             complement(76774..77703)
                     /gene="kdgK"
                     /locus_tag="LGK42_000067"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBK2656359.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            77935..78702
                     /gene="pdeH"
                     /locus_tag="LGK42_000068"
     CDS             77935..78702
                     /gene="pdeH"
                     /locus_tag="LGK42_000068"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBK2656360.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            78772..80832
                     /locus_tag="LGK42_000069"
     CDS             78772..80832
                     /locus_tag="LGK42_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBK2656361.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(81066..82388)
                     /locus_tag="LGK42_000070"
     CDS             complement(81066..82388)
                     /locus_tag="LGK42_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBK2656362.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(82799..83812)
                     /gene="yhjD"
                     /locus_tag="LGK42_000071"
     CDS             complement(82799..83812)
                     /gene="yhjD"
                     /locus_tag="LGK42_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBK2656363.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(83861..84760)
                     /locus_tag="LGK42_000072"
     CDS             complement(83861..84760)
                     /locus_tag="LGK42_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2656364.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            85280..85882
                     /locus_tag="LGK42_000073"
     CDS             85280..85882
                     /locus_tag="LGK42_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK2656365.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(85933..87582)
                     /locus_tag="LGK42_000074"
     CDS             complement(85933..87582)
                     /locus_tag="LGK42_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBK2656366.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            87987..89384
                     /gene="ccp"
                     /locus_tag="LGK42_000075"
     CDS             87987..89384
                     /gene="ccp"
                     /locus_tag="LGK42_000075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBK2656367.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            89595..>90610
                     /locus_tag="LGK42_000076"
     CDS             89595..>90610
                     /locus_tag="LGK42_000076"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBK2656368.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREG"
BASE COUNT        21883 a        22721 c        24018 g        21988 t
ORIGIN      
        1 gtatttctgg tgatattcta tgggattatt attttacatg atattcccta cctgattgct
       61 aaaaaacgta atcatcctca tgccgacgcc attcatgttg ctggttgggt gagtcttttt
      121 acgttacatg ttatctggcc atttttgtgg atttgggcca cgctttaccg cccggagcgg
      181 ggatggggaa tgcaaaacca tgactcctcc gttgtgcaac tacaacagcg tattgccggg
      241 ctggagaaac agctcgccga catcaaatcc tcttctgccg agtaatactt atggatttac
      301 tgattatttt aacctatgtg gctttcgcat gggcaatgtt taagatcttc aaaattcctg
      361 taaataaatg gaccattccc accgcggccc tgggtggcat atttattgtc agcggtttaa
      421 ttctgttaat gaactataac catccgtata cctttaaagc gcaaaaagcg gttatttcta
      481 ttccagttgt cccgcaggtg acaggcgtgg tgatcgaagt gacggacaag aaaaatacgc
      541 tgattaaaaa aggcgaggtg ctttttcgac tggacccgac gcgttatcag gcgcgggtgg
      601 atcggctgat ggcggatatc gttaccgcag aacataaaca gcgggcgttg ggcgcagagt
      661 tagatgagat ggcggcgaat actcagcagg caaaggccac gcgggataaa ttcgctaaag
      721 agtatcagcg ttacgcacgc ggcagccagg cgaaagtaaa cccgttttca gaacgcgata
      781 tcgatgtggc gcggcaaaat tatctggcgc aggaagcctc cgtgaagtca tcggcggcgg
      841 aacaaaaaca gatccagagc cagctggata gcctggtgtt gggtgaacat tctcaaatcg
      901 ccagcctgaa agcacagctc gcggaagcaa aatataacct tgagcagacg atagtgcgtg
      961 cgccgagcga tggttatgtg acccaggtac tgattcgtcc gggtacctat gccgcgtcgc
     1021 tgccgctacg tccggtgatg gtgtttatac ccgatcagaa acgacaaatc gtggcgcagt
     1081 tccgtcagaa ctccttgctg cgactggctc ctggcgacga tgcggaagtg gtgtttaatg
     1141 ctctgccagg taaggtattc agcggtaagc tggcagccat tagtccagcc gttcccggcg
     1201 gagcttatca gtcgaccggc accttacaga cgttaaacac agcgccgggt tcagatggcg
     1261 ttatcgcgac cattgaactg gatgagcaca ctgatttgag cgctttacca gacggtattt
     1321 acgcccaggt ggcggtttac tctgatcatt tcagccatgt ctcggtgatg cgcaaagtgc
     1381 tattacgcat gaccagctgg gtgcattacc tttatctcga tcattaatct tcgctttccg
     1441 ccgcttgttc aggccgcagt tgctgcgact gagcaagctg gtggcgaatt tcacgcagca
     1501 tttcatccag cacatactgg acccgccagg gctggtattc ccgtttacgg aagatagcca
     1561 ccagatccag ccaccattca tagggttgct gaaagcaggg caccagcgtg ccgtcttgca
     1621 ggtcgctctg cacacttttg tctggcgcaa acacaatccc caggtgattt cgtgccagct
     1681 ccagcgcaga ttgcgtgttg tcgcaaacgt aatttccctt cacgcgataa tctcgcacct
     1741 ctttactccc ggcgacgttg aagcgccaga tattcgcgtc atcaatcatc atcgagtcga
     1801 tcaaaataca ggagtgatgt tcgagttcgt caggacggct aatgggatgt ttctcaagat
     1861 agcgctggct ggcataagcg gttacggcat attgggtaat aaaactggca accagcgatt
     1921 catcttttgg tggagcgtag ctgattaaaa catcacaatc atcaggaaat tcgacgcctt
     1981 cataaaaggc attacgctca agattgcagg tttttagcga taaggtgata tcaccgatat
     2041 ctttaatttt atcgattaca tgtttggata aataggtaat tatgccggtt ggggcgtaga
     2101 tggtgacccg accacgtttc tcatgcttat aatctgcaat aaaattatta agctgctcgt
     2161 ttctgtccag catgtcgttg atgtacggta acagcgcggt accaaaaggt gtgagcatca
     2221 gctgccgggt ggttcggtca aagactttta aacccacttt tgattcaaaa tcagcaagat
     2281 atttgctgac gttggcctgt gcgatgccaa gtacagtcgc ggcatggctg atattttcac
     2341 tggcagcgat taccgagata atttttaact cccggtactt aagttgtaat tttgtcatcg
     2401 caacagccca attatatata attttatata ttactatata aatatgaatc ttgcaccgga
     2461 gaaattgtaa gcattactat gccgactctt ttttatctta ttttaaaatg gaataccgaa
     2521 catgttaatt aaccgcaata ttgtggcgtt atttgcgttg ccttttatgg caagcgcaac
     2581 tgcttctgaa ttatccattg gtgccggtgc ggcttataat gaatcgcctt atcgcggtta
     2641 taatgaaaat acaaaggcaa ttcccctgat tagttatgaa ggtgattctt tttatgttcg
     2701 tcagaccacg ttaggtttta ttctgtcgca aagtgaaaaa aatgaactta gcctgaccgc
     2761 atcctggatg ccgctggaat ttgaccctgc cgataatgac gattatgcca tgcaacagct
     2821 tgataagcgt gatagtacgg ctatggcggg ggttgcctgg tatcaccacg agcgttgggg
     2881 aactgtgaaa gcctctgcgg ctgcggacgt tctggataac agcaacggct gggtggggga
     2941 gctatcggta ttccacaaaa tgcagatagg tcgcctgtcg ctgacacctg cgctgggcgt
     3001 tctctattat gacgagaatt tcagtgacta ttactatggc atttcagaga gtgagtcccg
     3061 tcgtagcggt ctggcaagtt attccgcgca ggatgcctgg gtgccctatg tcagcctgac
     3121 ggcaaaatac ccgataggag agcacgtcgt attgatggcg agcgcaggat acagcgagct
     3181 gccggaagag attaccaaca gcccgatgat tgatcgtaat gagagtttta cctttgtcac
     3241 cggcgtgagc tggcgttttt aattcaccgg tggatgtcgg tgcggctggc ctacgctctc
     3301 gcaccgactc gtatgcctga taagacgtgt taagcgtcgc atcaggcaat gtgctcccta
     3361 cgttggatgc ggcgcgagcg ccttatccaa cctccggatg gcgatgcttt tcctcattca
     3421 cccagatccg catttcgcct tcgccccggt tagcccagct aaaccacggg ataaaggtca
     3481 gagtttgcgg ctggcgtttg gctggcgcgc tgtcgtaatg ccacagcggt tgctgctctg
     3541 gattgctctg ttcataccgg taacccggtg cctggattaa gatcttatgg ctaaacaatc
     3601 ccttgccttc aaatgtcgta aatggcgcat cggtgggcag ccacagatta tgcagtgact
     3661 cgccgttgtc ggcctgttcc aggcaataca ccagcgggcc gcgctgaatc gccacttttc
     3721 cggcgacgtg acgcaccagc gggttaccgt aaacgcggcg taccggcatc ggcaaagtca
     3781 gattcagcgt atcgccctcc tgccattcgc gggtaatgtg caaataccct ttacgaatat
     3841 cctgctcgac ctcttcccca ttcaatatga tctgcggctg tgtgcaccag tccggcagac
     3901 gtaaagccag cgtatgacgc accggctggg gcgattcgac cgcaattgtc acctgctcct
     3961 gccacggata gttcccgcta acccgcaggc gcagtgtgcc attttctacc ggcacttcca
     4021 tgctgtttcc tgcgtatatg ttgatataca acgcatcttc acgcggcgtg tagagataat
     4081 gaccaatcga ggtcagcacg cgggcgatat ttggcggaca acaagcgcag ccaaaccaac
     4141 gctggcggat cggtttaacg tgatcgtaga tatggttgaa tttcagcgat tttggatgta
     4201 cttccagcgg attcacatag aagaaatgtt tgccgtccag cgccatgccg ccgagcacgg
     4261 tgttgtacag tgcgcgctcc atcacatcgg catattgact gtcgccttcc atttccagca
     4321 ttcgtcgggc aaacatcatc aggccgatgg aggcgcaact ttcagcgtat accgtgtcat
     4381 tcggcagatc gtaatcgctg ctgaacgctt caccgctgct ctgtgagcca atgccgccgg
     4441 taatatataa ctgacgctgg gccatattgt tccacagccg caggcagtcc tgacgcttgc
     4501 tgtcatcgtg acttaaacgc gcgagatgcg cgacgccggt catcagatag acaaaacgta
     4561 ccgcgtgacc gatggcggtt tgctgttgcg caaggggcaa atgtgcctgg ctgtaggctt
     4621 tgtctttcac catccacgcc gggccgtagg tgtgccagtg cgaggtctgc ccgcgctttt
     4681 catattcctg gtcgtaatag tgcggttgca caccacgctg ttcgacaaaa tagttcgtca
     4741 gcgccaggta gcgcggctct tcggtgactt cgtagagacg catcagcgcc agttcaattt
     4801 ccggatgacc gggataaccc tgcagcttgt cttcatctgg accaaatacg cggtcgatat
     4861 gatcggccag acggcaaacc acctccagca agcgtcgttt tcccgtggcc tggaagaagg
     4921 cgactccggc ttcaatcaga tgaccggcgc agtaaagttc atgacactcc gcaagattgc
     4981 tccagcgttc ttcgggtgct tttaccgtaa agtaagtatt gagatagccg tcttcgcatt
     5041 gggcggaggc gatcagttcg attacctcgt cggcggtttt ttccagttcg gcgtccggct
     5101 tctggcacag cgaccaggct accgcttcca gccatttggc gacgtcgctg tcctgaaaca
     5161 ccatcccgta aaattcaccc tcctgaagtc cggcggcaat gcgaaagttt tcaatcgcat
     5221 ggctgggttc cgcttctggg atacgatcgt tcaaggcatc ccactgataa gaaatcacca
     5281 cgtcgcggac cagttgttgg tactgaccga ggaacggatc gctgaccgtc agtttacgca
     5341 gatcgacttc cgaaatgttc atcttgtgct ccttaaggct gtacatgacg ctgacgcagg
     5401 tcggtagcta tgcgcgacat cataggatta ttgagtttgc aaaagcggat cgcccctgcc
     5461 agcagcaggt ggaatagcgc aggcagcaat gtttccatcg ccgtgatccc gccaagcgaa
     5521 ctggcggtct ggttttccac gccaggctgg taggcgacaa aaataaacag caggctgatg
     5581 atcccggcgc tggaggccca ggctagcttg atgaaaaaca gattgaaggc gaagttcatg
     5641 ccggaagaac gtacgcgggt tttccactcg ccgtagtcat cggcaaaggc catcaatgag
     5701 aagtgcaatg gcaatgtgaa gccaagaatc acgccattac cgaggatcac cgccagccac
     5761 agcgtttgat aagccgggcc ggagggcagg aaccacatca acaccgccag tgcagcgagg
     5821 agcaggttgg tgtagtaata aattttgacg gtatcgaaac gccgcgttaa cgggctgaca
     5881 atcaccgagc cgataatgga ggcgaaggtg accatggtga agaacagcga cgtgtagccc
     5941 gtactgcctt gtaagacgta ggtaatgaag tacatatacc caccgccgcg aatattaaag
     6001 acgttaatca gcaggaaaga catgaccagc atcagcagca gttggtcgtt gttccgcagc
     6061 ccggcaagat gttcgcgcag ggtaaatttc cccattgtgg agagcggcac ccgttcacga
     6121 acccagaaaa aacagcacag gaacatcacc acggcaatgg cgcacaatac gccgacgccc
     6181 agttgatagc cgcgtgcagc gttgccctga ccgaagagat ctaccatcca cggtaagcct
     6241 accgaaacca gaaatcccgc tacgccgcag agaacgaatc gccaggactg gcaggagatc
     6301 acttcattgt ggcgggtggt catggtgttg atcagcgcgc agtacggtac gttgatggcg
     6361 gtataaccga ctgaaaggag caggtaagtg ccgaacgccc aggtgatttt tactcccatg
     6421 ctggcatctg gcacggtgaa cgtcaggatg ccgataatgc cgatgggaat ggcaatccat
     6481 aactgccacg gacgaaagcg cccccagcga ctttgcgtcc ggtcggcaat gacgcccatc
     6541 accgggtcgg agatggcatc aaacacgcgc agtgcaataa acagcgtgcc aaccagcgcc
     6601 ggagttaatc caaaaacgtc ggtgtagaaa aaggtgagaa agttcatgat caggcaagtt
     6661 attacggtgc cgccagcgtc acccaggccg tagcctattt tttcccgtac agataacgca
     6721 tcgtcaattt tacctttagc gatatcagcg gtagtaatcg gtgtagaggt catagatatt
     6781 cctcgtgaat aggaagcaat ttttagtttt attgcaatga cctgattaat tctgccgtga
     6841 acgggaatat caacaaggtg agaaaagagt aaaaaaaggt gacgattccg acctgaattt
     6901 aaaagtgtga aatggatcag gtgaaattaa tgtttttgat taataatcaa aaggtaatgt
     6961 gtttaatcat taatgaataa gcgggaaatt acatatcaat gctcgaatta tccatgacgt
     7021 taccgattaa agtgcaaaat ggtggattat ttatttcccg cggcgtaggg aggcatcctg
     7081 caaggcggct ggagtcatgg gaaattatct ttgtcgaaaa aggtcgatta acgattcagg
     7141 aagaagaacg gatatttctg gtcgatgcgg gagagagttt attactctgg ccgaatcgac
     7201 aacatgttgg ggtggaggat tttcccgccg atctcaaatt ttactggctg cattttgagg
     7261 ttaaagcgcc ggatagcgac cctcgctggc ttactcatct tagcgtgccg caacaaacga
     7321 aggttgtcga tccgcaggcg ctggtggccc tgtttcgcca gttcttaagc gaacaggaaa
     7381 agcatcaacg cactccggcg ctggagttta ttgtgctgct gattttgcag cagctcaccc
     7441 gtgacgcgcg tcttgatgaa caggccgatg gcgcaggcac cacccttgcc tggaaagcgc
     7501 agaaactgat ccgcacgcaa tatcatcgcc cgctttccac ggcgctttta gcccgcgagc
     7561 tgtactgcaa tgccgattat cttgggcgcg tctaccggcg ggtgtttcat ctgacgttaa
     7621 cagaagcaat ccatcgccag cgagtgcgcg aagccgaaaa gctgttaatt agcgaagcgc
     7681 gatcgctcac tgaagtggct gcgttgtgcg ggtttaatga cgttggctat ttccggcaga
     7741 tttttcgcaa gcacaccggt ttaacgccag cagcgtggaa gcggcgttac agcaaggaac
     7801 atatcaattc gtagtgccgg ggcgatgaag ccccggcgtg agggattact gcccgtaata
     7861 ggcattggcc ccatgcttac gcaggtagtg cttatccagc agttcgtttt gcatcgcagg
     7921 gagctgcggc gcaagctggc gcgagaatag gcccatatag gcgcattctt cgagtactac
     7981 ggcgttatgc acggcatcgg cggcgttttt accccatgcg aacgggccat gagaatgcac
     8041 cagcaccgcc gggatttgtg ccggactcct gccacgttct tcgaaggttt caatgatcac
     8101 ttcgccggtc tgatattcat attcgccgtt aatctcctct gcggtcatct gtcgcgtgca
     8161 ggggatggca ccatagaaat aatcagcgtg cgtggtgccc caggcgggga gatccagccc
     8221 ggcctgcgac cagatggtgg cgtggcgtga gtgggtatgc acaatgccac cgatttcggc
     8281 gtagcgacgg taaagcgcca gatgcgttgg cgtgtcggac gagggctttt tgctaccttc
     8341 caccaccttg ccgctggcta tctcaaccac caccatatcg tcggcggtca tcacgtcata
     8401 ctcgacgccg gaaggtttga ttaccatcca ttgccgcgtt tcgtctaccg cgctgacatt
     8461 gccccaggtg aacgtcacca gatggtgagc gggaagcgcc agattcgccg ccagcacgtc
     8521 ggctttcagt tgctctaaca tataaatcca gcctcctgca tacgcgcttc aatccaacgc
     8581 cgcgcctgaa taatctccag caccggctct ttggcttttt cggtccacat ctcaatcagg
     8641 aaagaaccgc gatagttcag tttatgcagc gttttgaaga tgccaacgaa atcgacgcag
     8701 ccttcgccaa acggcacgtc gcggaactgt ccgggacttt gctcggtaac tggctgggtg
     8761 tctttcaggt ggatcgcggc gatacggtca atgcccagtt tcagttcggc gggaacatca
     8821 ttgccccagg cgctgaggtt gccaacgtcc gggtagacgg tgaaccacgg cgaggcgagc
     8881 atctcgtccc actttttcca tttactgatg gagttcataa acgcggtgtc cataatctcc
     8941 accgctagca tgacttgtga tgccgccgcc tgttcgaccg cccacgccag cccttcagca
     9001 aaacgttgcc gggtgccttc atcgtggtct tcgtaataga cgtcgtagcc cgccagctgg
     9061 atggtgcgta tgccgagatc gcgtgccaga cgaatcgctt tgctcatgat ctcccgtgct
     9121 cgctggcgta ccgcgtcgtc acggctacca aaaggaaagc gacgatgtgc ggacaggcac
     9181 atcgacggga tcccaacacc cgtttcgatc atcgcggcaa ccagcgaagt cctttgtgcg
     9241 gtgctccagt caagacgtga aagccgttcg tcggtttcat ccaccgacat ttcgacaaaa
     9301 tcaaaaccgc agctttttgc cagcaccagc cgctccggcc aggagagatc tttcgccagc
     9361 gccttttcat aaatccctaa cggatgatta cgcacgcttg cctccccaga tagcgtcgat
     9421 ttgcgcatgg aaatcgccag ccacctgcgc cggatttgct gagcctgcca gtgctcgccc
     9481 ggcaataaac gctttcacgc gaatatcttt aaacagcggc aggtcagcag gggtaatccc
     9541 gccggtaatg gaaagctcaa ggccgatatc ggaaagcgcc ttcatgcgtg ccagatcggc
     9601 ttcgccccac tgttgcccgc tggcctgtgc atcacgaccg cgatgataaa tcgcctgccg
     9661 cacgccaata cgatgccagt cgcgggcgtc gtccagcgtc cagttaccga acagctcgat
     9721 ctgaatttca ccaccgcagc gttgcgccat cgcgtggcct ttttccaccg tcgcgagcgg
     9781 tgcggcgcag atgatggtca tccagttagc gcctgcgcca aatgcctgtt gcgcgagcgt
     9841 ttcaccagcg tcggcgacct tccagtcggc aacgatgatt ttgtcagggc actgttcgcg
     9901 caaggctttc accgcgccaa gcccttcgtt taaacagaga atggtgcccg cttcgacaat
     9961 atcgacgctg tcttttaact gcatcacgtc gcgctgtgcg gcttcaagtg atgagtggtc
    10021 gagtgccagt tgcagaagtg gtcggctcat aatatgtgct ccttgatgcg ggcgtgaaag
    10081 ccctgaagtg cggcaatgag atgctgatag cgttggtatt tttgttggta aagctgatgg
    10141 gcggtcatat ccggcagcag ggtgcgtacc gggtgctgta agtcacgttg agcttcgctg
    10201 aaatcgcgat aaactccggt gccgacacgg gcggcaagtg ccgcgccaaa acagccggtt
    10261 tcttccacct gcggcagctc gatacgcaga ccgctgacgt ccgccagcat ttgcatccag
    10321 acatcggagt gcgccgggcc gccagtgacg cgcagggtgt gcacatcagt aaaacgttcg
    10381 cgcatccggt tgaggtgggt catatggctg aacaccaccc cttcataaat ggcctgcaac
    10441 aggtgtgcgc gggtgtgaat ggcctgcatc ccgtagaaac cgctggtcat ctcgagtccg
    10501 gcgttgctgc cgtacaggaa cggcaggaaa aagagatcgc caccggcctt cggcaagctg
    10561 gcaacggcct ggttgatctc atcaaacgag atttctcccc actgtgcggt aaaccattcg
    10621 aggttgccgg aagaggtggg gctggcttcg tgaacgataa attcaccatc gttaacgtag
    10681 cgaccataga catacggatg cgcttcaccg tcacgtaaac cgcgggttat gccgctggtc
    10741 accgcccagg tgcccatcac cgcattaagg gtaaattcgt cttcgatccc ggcgcagagt
    10801 gcggtggaaa ccacatcaaa caggccgccg acaacgggcg tacccgcttt cagaccggtc
    10861 agtacggctg tctgagcggt gatctccccg cagatttcgg cagatccgac aacaggcggc
    10921 agggcgtgat tgatttcagc aatccccagc cagtcggtga ggcacgggtc atattcccca
    10981 agactcatgt tgtagaggtt ggactcggaa atattgctct cttcacagcc tttgacgccg
    11041 gttaaacacc agcgcaggta gtcgtgcgtc atcatcacgc agccaatttg cgcgtagcgt
    11101 tccggttcgt gctctttcag ccagcgtaac agcgacaccg gatgcccggt ccacaaggtt
    11161 tgtcgggtca acgggtagag tttttccggg atgccatctt cctgccagcg acgaacgatt
    11221 tccatcgccc ggcgatccga ggacaaaatg gcgttaccga gcggcttgtt gtttttatcc
    11281 agcaaaaaca agccttttcc ttgtgcggag ataccgatac cgacaatttg ttccccgcta
    11341 acgccggaat gggtaagcag ttcgcgaatg acagccatgc agcattgcca cagttctgcc
    11401 atatcgcgct ctgcccaacc tggctgcggg cttaatgcgc acagcggcag gcgctgcacg
    11461 cctgcctccc ggccttcgcg gtcatacagc ccggctttca gccagctacc gccacaatct
    11521 aaccccagcc agtattgcgt catggtttat tgcacctcat ccacggcttt cagtagatca
    11581 gcaccctgcg gaacatcttt tacaaacatg tcgcggacct gagtacccag tgcgtcgcta
    11641 aaggctttgc ggtcgagatc ggtgatgact tccacgcccg cttctttcat gccgtcgatg
    11701 attttttgct gatcttcggc aaccagtttg cgctggtagt tacccgcttc ctgcgcagat
    11761 gaaattagcg cttgctggaa ctccgggctt aagccatcaa acttcgcttt gttgatcacc
    11821 accagaagcg gggaataggc gtggtgcgtc agagaaaggt acttctgcac ttcgaaaaat
    11881 tttgctgacc agacgacgtt gatcgggtgt tcctgagcgt cgatagtgcg ggtttccagc
    11941 ccggtataga cttcggcaaa cggcatcggg atcgggttag cgccaaagac tttgaatgcg
    12001 gcgatattca tcgggctatt gttggtgcga attttcagcc ctttcaggtc ggcgggggtt
    12061 ttaaccggtg cgcgcgagtt ggtgacatcg cgccagccgt tttcccagta ggccagtact
    12121 tttagtcctt taccttcaag tgaggctttc agatcatcac cgactttgcc gtcgagcgtt
    12181 ttatgcgcgt gagcggtatc gcggaacagg aaagggacat caagcaagtt catcactggt
    12241 gataacccgg caaagttatt cgagccggac atttccatat cgatggtgcc gccacgtacg
    12301 ccgctgatca tcgcctgcgc gttaccgagg gtgctatccg gaaacaattt taatttcagc
    12361 tcgcctttgg ttctctcctg caataaatca ttgaattttt tcgccgcaat atgttgcgag
    12421 tcggtttgtg aggtttcata accgaaacgt aaagattgcg ccgccagaga agatgtgctg
    12481 aatgcagcca ggccagcaat gaataatgcg taggttacag agcgtaattt cataatattt
    12541 tccttttaat taatccattt caaagggagg atgatgagat cgggaataaa aacaaatacg
    12601 actaataatg aatataaaac caggacgtaa gggaaaacgc ctctgactgc atcatcgaat
    12661 ttgagttttg ccacccctga aataacgtta agtacattac cgataggcgg tgtaattaag
    12721 ccgattgagc agttaatgat aaacatgaca ccgaaataaa tcggatcgat tcctgcctct
    12781 ttaactaaag gcattaatac cggggtaaga attaataccg tcggcgttaa atccatgacc
    12841 atgccgacaa tcaggatagc caccataatg acgataaaca gcagacgcgg tgaatcgacc
    12901 aacggttgca gtaaatcaga aaccatcatc ggcagttcag caatggtaat cagccaggcg
    12961 gaaacttgtg ccgaggccac cagaaacata accactgagg tggttttcgc cgcgccaatg
    13021 agtacatgcc aaagtgtggc gaaggtcatt tcacggtaaa taactgtggc gacaaacagc
    13081 gcatagaaag cagcaacggc cccggcttcg gtaggggtaa acagaccgga gcgaaacccg
    13141 ccaataatga tgactggaag aaacagcgcc cagataccgg agacaaaaga gtgccagatt
    13201 tcctgcatcg ttgctttttg ctggcggggt aaattaaggc gactggcctg ccaccaccag
    13261 gtaagcatca gcgttgcgcc catcatcatg ccaggggcaa tgcccgccat aaacagctta
    13321 ctgatggata atccgctgga aacgccgaag ataataaacg gaatggaagg gggaataatt
    13381 ggcgcgataa tgccgccaga ggcaatcagc cccgccgccc ggttgaccgg gtagttggcg
    13441 ctgcgcatca tcggcaccag tagagcggca acggcggcgg tatccgcaac ggcagaacca
    13501 gaaaggctcg ccataatcat agctgccagc acgccgacgt agcccagccc gcctggttta
    13561 tgccccacca gtttcatcgg caggtcaaca atgcgttttg acaagccgcc cgcattcatg
    13621 atttcacccg ccagcacaaa gaacggaatc gccagcaggg agaagctatc ggctccgttc
    13681 accagcgttt gtgccatgat ctggacatca aacatgtcca gccagaacat taacgccgcc
    13741 ccgcacaaca acagtgccca ggcaataggc aaaccgatag caataccacc caacagacag
    13801 cccagaaaaa tcagcacagc catgattaag ctccttgcgg tggcgacgtt agagaattgc
    13861 tacgcgtgat gagttgatat aaatgacgca gttcaaagaa tgcgataacg aggctggtgg
    13921 gcagacaagc ggcatacatc aggccgatgg gtaaaccgag gatcggtgaa taatcgctcc
    13981 agtcctgaat tgtttttagc gttgcgcccc aggccagtgc gccacaaata aataagatta
    14041 aggaatgggt aaccagagcg actcgtcgtt gccatgcggg ggagagtttc tccaccagaa
    14101 aggtgacctg aacgtgggcg ttatccataa aagccacaat cgcgccaata aatgttaacc
    14161 agacaaataa ataacgtgac aattcatcta cagataaaat gcttgtctga aaaccatatc
    14221 ttaaaataat gtttataaac acaatacagg aaagtacggc gagattaatc gccagtattg
    14281 cttcaagtat ttttttcata gctattcctt gaggctatgt ctgtcataat tcaagttgca
    14341 tgtgcagcaa cctaaattat taatatagtt agagcaataa cattacccag tcgttctggc
    14401 gtagacgatt attcgattaa ttcagcgccg ttaatgcgac tttaaccaca atttttcgta
    14461 tctcagaggc tgtttgcaaa atacaacctg gtcggtgaac atcctgcgga aagaaaattg
    14521 cgtagctgcc cggtatcatt tctataaatg attcatgttc actgtcatga taaaaaataa
    14581 tatcgcgctg ctctaatagt gattcgctga ctttattatt tcccgtatca atagcaatgc
    14641 cgattttctc ttcaccccac gccagaaact gaatatcgat ataccgacga tgcacttccg
    14701 gacggttttc caccgcttcg cgtgtggtta aatcgataat ttgcgcataa atatttttgc
    14761 cgtcgatttc gacaacgccc ggctccaggg cgttgaaatc ggtagcgcgc agaaaatcga
    14821 gccccttttc aatggcggcg ggcaaacggc acggattggg ctgcgcgata tgtccaaaaa
    14881 tcatgactta tctccttata acgcctggat tttggcccac acgctgtcat caacggtaat
    14941 accgttacgg cggttttcgg ccagcagggt agtaaattcg tggccgggta agcggatggc
    15001 ctggttttcg tcagcgcgtt cagcggtagt aacgtaatcc atgatacgtt gcagcttggc
    15061 atcgcgggtg ggaccgtcga taagcttgtc cacttcaatg gcaataaaaa tttgtgaaat
    15121 gccgtattcg tcgctgttgt cctgggtgac ttcggcaacc gatgcgccgt cggaaaggag
    15181 agtagcgatc atatccagca caatcgacat gccagaacct ttccagtagc ccatcggcaa
    15241 aatgcggcga ttcttctcga taacgccagg ttctttggtc aaattgccct catcatcaaa
    15301 gccaccatcg accgggagct gacggcccgc caggcggtta acttctaaca tgccgtaaga
    15361 gaacatcgac atcgacatat cgaccatggt gatcggcgtg gaaggaatgg cgacgatcag
    15421 cgggttagtg ccaatgcgac actcttttgc gccccacggc ggcattacgg cgatggagtt
    15481 ggtccagcaa atgccaatat agcctttttc cgccgcctgc cagccgtagc tgccgccgcg
    15541 catccagtgg ttggcattac gaagtgccac cagaccaata ccgtgatcgg cagccagttc
    15601 aatggcgcga tccatcatct ttttcgccgt caggttgccg atcgaacgct gggcatccca
    15661 ctgttcaatt gcgccgaggc tggttatacg tttgggttgg gcatcaggaa tgatatcgcc
    15721 gttttccagt tgttgaatga aacgagggaa acgattaacg ccgtgagaat aaacgccgga
    15781 ttcggtggtg cgggcgaaca tctctgcaca ggcgtcagcc gtttcgctgt caacgccgcg
    15841 tgaaattaag acccgattaa aggctgcttt taactgctca aatgtcactt tcatcccagg
    15901 cttccttgtt tttttgatcg cttttttggc ttaaaatttc aatatgcgaa acttgatttc
    15961 aaatatatcg atcacttttt aacaaagcaa tctgattgat gaatttcaaa agacataaaa
    16021 atcaagtggt tataaattat ctgtcgagat cgtgaactac ggcacacttt gcgctaccat
    16081 caggacgcga caaaatgggg aaagaagtga tgggaaaaaa agagaacgag atggcgcagg
    16141 aaaaagagcg tccagccgga agccagagtc tgtttcgcgg gttgatgctg attgagattt
    16201 tgagcaacta tccaaacggt tgtccgttgg cgcatctttc ggagctggct ggtttaaata
    16261 agagtaccgt ccatcgctta ttgcagggat tacagtcctg cggctatgtg accaccgcgc
    16321 ccgccgcagg gagttaccgc ctgactacca aatttattgc cgtcgggcag aaggcgctgt
    16381 cttcgctgaa tatcattcat atcgccgctc cgcatcttga ggcactgaac atcgccactg
    16441 gtgaaaccat taacttctcc agccgcgaag acgatcacgc tattttgatt tataagctgg
    16501 aacccacaac cgggatgctg cgaacccgtg cctatattgg ccagcatatg ccgctctact
    16561 gttccgcaat gggcaagatc tacatggcgt ttggtcaccc ggactacgtg aagtcatact
    16621 gggaaaacca tcagcatgag atccagccgt taacccgcaa taccattacc gagctgcccg
    16681 cgatgttcga cgaactggcg cacattcgtg aaagcggagc ggcgatggac agagaagaaa
    16741 acgaactcgg cgtctcctgt attgctgttc cggtgtttaa tattcatggg cgggtgccgt
    16801 acgccgtgtc gatttcgctt tcgacatcac gtctgaaaca ggtgggggag aaaaatctcc
    16861 tgaaaccact gcgtgaaacc gcgcaggcta tttctaatga actgggattt actgtcaggg
    16921 acgaccaggg cgcgatcaca taacgctttg gacaagtgcc aaaactttaa catttccttc
    16981 gttggatcaa agcagtaggg acgcgctctc tggcactctg ctgttttagt gcaaaggagt
    17041 gatcatgaac cggtttatta ttgcggatgc gacgaaatgt atcggttgcc gtacctgtga
    17101 agtggcttgc gcagtgtcgc atcaggagaa tcaggattgc gctgcgttgt caccagacga
    17161 gtttatttcc cgtattcgtg tcattaaaga ccacagctgg accacggcag tagcctgtca
    17221 tcagtgtgaa gatgcaccgt gcgcgaatgt ctgccctgtt gacgcgataa gccgcgaaca
    17281 tgggcatatt ttcgttgaac aatcacgttg tattggctgt aaaagctgta tgctggcttg
    17341 cccgtttggt gcgatggagg tcgtttcttc gcgcaaaaag gcgagggcga ttaagtgcga
    17401 tctgtgctgg catcgggaga cggggccggc ctgtgttgaa gcctgcccga caaaggcgtt
    17461 gcagtgcatg gatgtcgaga aagtgcagcg gcatcggcta cggcagcagc ctgtttgaaa
    17521 cgtttttccg gcgtttctca acgccggaat agtgcgcttt gtttatgcca gatgcggcgt
    17581 aaacgcctta tccggcctac aaattcttgc aaattcaata tattgcagaa atcatgtagg
    17641 cctgataagc gtagcgcatc aggcaatttt tagtgacttt cagcccaggc tctttctatc
    17701 tcttccgcca gaatcttcac ccccgcctca attttctccg gctctggtac gtagttcatg
    17761 cgcatacatt gatgcgtatg cggccacggt ttatccagcc ctgggaagaa gttgtgcccc
    17821 ggcaccatca gcacgccgcg tgctttcagg cgctggtaga gttgctcggt cgtaatgggc
    17881 aaatccttaa accatagcca gaggaaaatg gctccttctg gtttatgaat taggcagcga
    17941 tcttccggta aatagcggcg aatgatggcg atagtttcct gaacacgctg gtagtaaaac
    18001 ggtttgatga ctgtttcaga caggcgcagc agatcgttac gcttaatcat ttcacacatc
    18061 atcgccggac caataccgcc aggtgccagg ctgataatgc cgttcatatt ggtgatggca
    18121 gtgatgattt tttcattggc gatgataatg ccgcagcggg agccaggcag ccccagcttg
    18181 gaaagactca tgcacagcac gatattcgga ttccatagcg ggcgcgcttc actgaagatg
    18241 atacccggga acgggacgcc ataagcgtta tcaatcacca gcggaatgcc gtgttgattc
    18301 gccagcgcgt caagcttcag caactcttcg tcagtaatca cattgcctgt tggattcgtc
    18361 ggccgggaga cgcaaatcat cccggtttct tcgccaatat gcagatgctc aaaatcgacg
    18421 tggtatttaa actggccttc cggcagcagt tcaatattcg gacgcgcaga gacaaacaga
    18481 tcttcttcca gtccggcgtc agcgtagcca atgtattccg gtgccagcgg gaacagcact
    18541 tttttgaccc gaccatcggc acggcgtccg gcaaacagat taaataagta gaaaaacgcg
    18601 ctctggctgc cgtttgttag tgcaatattc tgtgcttcga tatcccaacc caacttctcg
    18661 cgcagcattc cggcaagcag tgtgagtagc tccgttttcc cctgtggacc gtcgtaattg
    18721 cacagtgcat cagtcgcttt gccactttcc agcatgtcgg tcagtagcgt ctggaagtag
    18781 tcctgcattt ccgggatctg cgccggatta ccgccgccga gcataatcgc gccaggcgtg
    18841 cgtaaaccgt cgttcagatc ttccatcagc agcgtaatgc cggagtggcg ggtaaatttg
    18901 tcaccaaaaa gggagaatgt catagcggga tatctgtcga actctgtagc aaggaaggta
    18961 acaataacgc tacatttgct ggggtgcaaa tcggtctgtt ggagagtgag cggtgcttta
    19021 tcctgcaacg ctgattaggg ctgacatttt atcctggttg gcggcttccc gaggtactga
    19081 cgaacgggaa gccggaaaag tcactgttgc ccagcccaga tgaccagcac tttatcgtcg
    19141 ccatgctcac gaacaaagcc gtagccctgc ttcagcgaaa gtgtcgtttg tttgcccgcg
    19201 ccaattgcgg gatggcgggc gcggaactgg ctgattttct gccagtgcgc gacgttggcg
    19261 gcagatttac cgctaacatc ctgccagttc atatccgaac gtgtaccttg cagcggatca
    19321 gaacctgtag gaccgaacgg acgcgaggat tcatcgccat aaaagatttg taccgcgcct
    19381 ggcgctaata gtaataactc tgctgctttg tcgccccctt cacggaacag acgggtatca
    19441 tgcgacgaga ggtagctcaa cacgttgaaa ccctgcaatt tctccgccat ttgctgccag
    19501 gtcgtatcca tctgcgccag acaatcgact gctttcgccg cctgctcctg ataatcgaaa
    19561 ttgatcatcg catcgaagcc gtggcgatag tagtcacttt gcatcacgcc gtggccccag
    19621 gcttcaccgg tcatccagaa aggtttgtca tctaatgctt tgtcggggtt agcttttttc
    19681 cattcgcgaa gcgcggcgct ggcttcggtt ttcagttgct gccaggcggg caactcaaca
    19741 tgtttggcgg tatcgacccg aaaaccatca atcccatagt cgcggaccca ctgacttaac
    19801 cagtgggtta agtaatcgcg aggtgtaaag ccttcgatga ctttagcgtg agtatccgtt
    19861 ttgtttttat agaacaccgg cagaccagaa gcggtagttg attcggtttt gatatccggc
    19921 aaaaaggcca gcgacatagt gagatcgtcg aatccaggat tgtcgtaatc gccgatatcc
    19981 gttctgatcc agttttttcc ccaccattta tcccagcctg ttttgtcgct gaaattaatg
    20041 taatcgttaa agctatgcca ggtttgcccg gcggcaggtt tccagtcgct ccagcgttca
    20101 cccagcgttt ttttcacctc gtcaccagaa agatataacg cgccaaactg atactcctgc
    20161 atatccgcca gcgtggcata gccggtgtgg ttcatcacga catcaaagag aatacgaata
    20221 ccgcgctgat gtgcgctatc aaccagcgtc cgtagatcgg cttcgttgcc catattggca
    20281 tcaagattcg tccagtcctg tgtgtaataa ccgtggtagg cataatgcgg gaaatcgcct
    20341 tttgtaccgc cgccgaccca gccgtgaatt tgctcaaatg gggcgcttat ccataaagca
    20401 ttaacgccca actgctggag gtaatccagt ttgttggtca ggccgcgtaa atcgccgccg
    20461 tgaaaagtgc caatttccgc cataccgtct ttatgacgtc cgtaactctg gtcattactg
    20521 ggatcgccgt tttcgaaacg atctgtcagc acaaagtaaa ccgtggcgtt atgccagtcg
    20581 aaaggggcag gggcgtcagt ttctgcccgt tccagcagga gtaaaccgtt gctggtagca
    20641 gcgggttgta acgttatttg accgttcttc actatcgcaa tttgctggct gtaataatcc
    20701 cgtacggcgg ctccttccgg gaaagtggcg ctgacatcca gcgtgagcgg taatccatcc
    20761 catttcgggc attcacggac caggtttgct accggttcgg cggcgttctg gatggaaatc
    20821 atcaatgttg gcgtaccgga gcgggtgtct atttgcagcg tatatttgcc gtctctgaac
    20881 aatcgccatt gaggcggcgt gttgctacaa ggttgcaggg aaagcatctg attgagtttt
    20941 atcgcatctg caggctgcca gcactgttgg tcaaaattta gcgtgagtgg acgcgtacct
    21001 ttgggcaact gcgcgtggct gacaaatgtt cccgttccct gttcgctaaa ggcagggaac
    21061 cccggagaag tccagctggc ggcaacggcg aagccaggaa ggagtgtcag aaaacaggcg
    21121 gcgagtttca tggtggatga gtccttattg ctgcgatttc agacagtttg ccagcgataa
    21181 gccagacaaa actcatccct gagcggtaag tccacaggat gagaagcaag ggtgagcgat
    21241 ctcgcgcaaa aaacggctga attttgcgat aaaccccaca ttttctgcga tttagcgcca
    21301 atctgaatcg ttaacacgtg acatagtttc agatttggac tattccttgg ggtgctcttg
    21361 acagctattt ttgatatgat ttgagattca actctcaaat ttgtgaaaaa tataaggtgt
    21421 tggaatgatt aaatccgacc aggagacctg atgattttga ctcccatacg acgatatggg
    21481 gcgatgattc ttatgttact cactctggtg ttttcgagtg aggtgttagc gaagacgcac
    21541 acaacaacag cgagtcaaaa gtcccactta actaaagcta gtaataaaca ggtaagcagt
    21601 aaacaagagt attctcgcaa tagtgcaaag agtagttcac ttcctgattt gcgaaaatac
    21661 ccttccggaa caccaaggaa aaaggcgttt ctccggaccg taatgcctta cattaccagc
    21721 caaaatgcag ccattactgc agaacgtaac tggctgattt caaaacagta tcagggccaa
    21781 tggtcacctg ctgagcgtgc gcgtctgaaa gacatcgcca aacgctacaa ggtgaagtgg
    21841 tctggtaata cgcgaaaaat cccgtggaat accttgcttg aacgcgtaga cattatccct
    21901 accagtatgg tggcgacgat ggctgcagca gaaagcggtt ggggaacgtc gaagctggcg
    21961 cgcaacaaca acaatctttt cggcatgaaa tgcatgaaag gacgttgtac caatgcgccg
    22021 ggtaaagtga aagggtactc acagtttagt tctgtcaaag aatcggtgag cgcctatgtc
    22081 actaacctca atacgcatcc ggcttactct tcgttccgta aatcgcgtgc gcagctgcgt
    22141 aaagcggatc aggaagtgac tgccacagcg atgattcaca agctgaaggg ctactcgacc
    22201 aaagggaaga gttataataa ctacctgttc gcaatgtacc aggataacca acggttaatc
    22261 gcggcgcata tgtaattgca tttccttgag ccttatctga tttgttggtc ggataaggct
    22321 tttgctcgca tcaggtggct gtgctgagtt ccctgatgtg acgccgacaa ttctcatcat
    22381 cgctacaaca tgacctcgct atttacatcg cgatactctt ttggcgttgt gtcatatgct
    22441 tttttaaaaa cagagtagaa atattgcagc gatggataac cgcacatttg cgatatctca
    22501 ttgatcgaca aggtggttga aatcagcaga ctgcgcgctt tctccagctt ctcggcatga
    22561 atcatggcat ggatggtttc acccacctct tctttaaaac gcttctcaag attggagcgc
    22621 gagatcccga ccgcatccag tacctgatcc actttaatcc ctttacaggc gtgattacga
    22681 atgtaatgca tggcctgaat aacggcggga tcggtcagcg agcgataatc tgttgagcgc
    22741 cgttcaatga cgcgaactgg tgggaccaaa attcgctgta gcggcatttc ttctttatct
    22801 aataatcgat gcaacagttt tgccgcctga tagcccattt gccgcgcgcc ctgagcgacc
    22861 gaagaaaggg cgacacgcga cagatagcgg gtcaattctt cgttatcgat gccaatcacg
    22921 cataattttt ccggtacggg aatatgtaga tgttcacata cttgcagaat atgccgcgct
    22981 cgggcgtcag taacggcaat aatcccggtt tgcggtggta gcgtttgtag ccagtctgcc
    23041 agccgatttt gcgcgtgttg ccagttctct ggcgcggttt ctaacccctg ataaaccact
    23101 ccgcgatact tttcttcggc gacaagctga cgaaatgcat attcgcgctc agtggcccaa
    23161 cgtttgccgc ttgattccgg aagaccataa aaagcaaagc ggttaacgcc tttctctttt
    23221 aaatgcaaaa atgcgctttc aaccagcgca tagttatcgg tggcaatgta atgaacgggt
    23281 gggtaacttt ctgcaaggtg atacgagccg ccaaccccaa caatggggac gtcgacatca
    23341 gccagcgctt gctcgatctg tttgtcgtcg aagtcggcaa tgacgccatc tcctaaccag
    23401 tccttgattt tatcaatgcg ggcgcggaaa tcttcttcaa tgaaaatatc ccattccgat
    23461 tgtgacgcct gtaaatattc ccctacgcct tctactacct gccggtcata ggctttattg
    23521 gcattgaaca gtaatgtgat gcggtgacgt ttagtaaaca tggttctttt cctgctgaat
    23581 catgcaaaaa ctcaaaaccg gtaatacgta accggctttg agaaaatttt aatcaaaatc
    23641 aagaacggcg tttggttgcg gagtccatcc atactgccag caacagaatc gcacctttaa
    23701 cgatatactg ccagaaggtc ggtacatcca tcatactcat gccgttatcc agtgaagcca
    23761 tgataaatgc ccccattact gctccggcaa cgcttcccac accgccagcc aggctggtgc
    23821 cgccaatcac gcatgctgca attgcgtcca gttcggcgat atttcccgca gaaggtgaac
    23881 cagcgccaag tcgagaacta aggattaatc cggcgatggc taccattaat ccgttaatcg
    23941 cgaacacggc aagtttggtg cgttcaacgt taatcccgga gagacgtgct gcttccagat
    24001 tgccgccgat ggcataaatg cgtcgtccaa atgccgtccg cgttgccata aacattccgc
    24061 cgagtaacag caacgtcagc agcagaacag gagtgggaac gccacggtaa tcattcaaca
    24121 gccagattgc gcctaatacg atgatagcgg ttaaagcctg gcgaccgact actgcggtag
    24181 aggccggaga ctgtaaaccc aaagcctgac ggcgcattct tccgcgccat tgccaaccaa
    24241 caaaagccat taagccaagc gcgccaatga tgaagccggt actggcgggg agatagcttt
    24301 gcccaatttg tgacatcgcg gcgctggtgg gggatacagt cgtgccgttg gtgatgccaa
    24361 tgagtatgcc gcgaaatgcc aacatgcccg cgagggtgac aataaatgaa gggactttgc
    24421 ggtacgcgac ccaccatccg ttccaggcac cgagaagcag tcccagaacc aacgtaacaa
    24481 tgatggtaag tggcaaaggc cagcctaacc agacgtcaca aatcgccgcg acgccaccta
    24541 atagccccat cattgagccg acggaaaggt cgatttcagc agaaattatg acgaacacca
    24601 ttcctaccgc gaggatgccg gtaatcgcgg tctggcgtaa caggttggag acgttacggg
    24661 cgcttaagta ggcaccatcg gtggtccagg taaagaacag catgattgcg atgatagctg
    24721 caatcatcac gaagacctgc aaattcagtg atttcagccc agaaaagcca ccggatgtcg
    24781 gtacggccaa tttcacttca gacggattgc ttttcgacat gatgttcgct cctcaatgcg
    24841 gcttccatca cctgctcctg agtcaggtta tgatttatca ggttggcttt tagtttccct
    24901 tcatgcatca ccagtacacg atcgctaagg ccgagcactt caggtaattc ggaagagatg
    24961 acaataacgg caataccctg ctggacgagt tggttaatta atttgtagat ctcatatttc
    25021 gcgccaatat caatacccct ggtaggttca tcaagaatga gaatgcgcgg gttgagtaac
    25081 agacagcggg cgaggatcgc tttttgctga ttgccgccgc tcaaacgtcc aatagcaaga
    25141 tcgggggacg acgttttaac tttgagttgc tgaattgatt ccagaataca tttttgctct
    25201 tccgcgtcat caagctggct aatgccaccg gtaaatttat tgagtgcggc gagggtaata
    25261 tttttaccaa ccgccattac cggaacgatg ccgtcgcgct ttctgtcttc gggtaccatc
    25321 gcaatcccct gggcgatggc ttgctgacag ttacgaatat ctacctgttt gccatcaata
    25381 taaatttttc cttcccattg tccgggccac acgccaaaca ggcactgaat ggtctcggta
    25441 cgtccggcac caacgagtcc ggcaataccc agtatttcgc cacgtttcag ggaaaacgag
    25501 acatcattaa ctcgtttaat atgacgattg accggatgcc atgccgtcag atgttcaata
    25561 cgtaatattt catctccggt ggtatgtggt tcattagggt aaagcgcggt taactctcgc
    25621 ccgaccatca tggtgataat atcgtcttca ctcattccgg cagcatcacg cgtaccaatg
    25681 tgctgtccgt cgcgaataac gcaaatcgta tcggaaatcg ctttgacttc gttgagtttg
    25741 tgcgaaatat aaatacaggc gataccgtgc tgttgtagat cgcgaataat atccagtaaa
    25801 accgacgttt cctgctcagt taatgaggct gtcggttcat cgagaattaa caagcgcacc
    25861 tgtttattaa gtgccttggc aatttcaacc agttgttgtt gcccaagccc taaatcgcca
    25921 acgcgggtat caggtgaaat ggataaactg acctgtgcga gcagcttctg acagcgtagc
    25981 gtcatcaggt cataatccat aatgccattg tgggttattt cgttacccag gaagatattt
    26041 tccagcacgg tcaattcttt caccagggcc aattcctgat gaatgatggc gatacctttg
    26101 cgttcggtat cgcggatgtg actcgcctga atctcttctc ccgcaaaaat aatttcgcct
    26161 tcgtaggagc catggggata aataccacac agcactttca tcagcgttga tttaccagac
    26221 ccattttccc cacaaagtga gacgatttcg ccagcattca accgcaagca gacgttatca
    26281 atcgccttca cactgccgaa ggttttggta atgttcttca tttcaagtag ataaggcata
    26341 acgactccac ctaagccaat tcattcacgc ggcatggaga gaaatcacgc ccccgctccg
    26401 cgccggggcg taacgcttac agctcgctct ctttgtggaa tccgtcttta actaccgtgt
    26461 ctttgatgtt gtttttattc acatcgatcg gtgtcaggag gcgggagggc acatctttca
    26521 ggccattatt cagtgtggta tctgcttttg gttcctgacc attgcccaac tcaacggcaa
    26581 tttctgcggc agtatttgcc aacaacgtaa taggtttata caccgtcata gtttgcgtac
    26641 cggcagcaat acgtttaata cctgcgagat ccgcatcctg gccggaaatt gctactttcc
    26701 ctgataaacc ttgcgcgctt aatgcctgaa ttgccccacc tgcggtggca tcgtttgagg
    26761 caactacagc atcaattttg ttattattgg cggttagcgc gttttccata attttcaatg
    26821 cgttttccgg taaccagcca tcaacccatt ggtcaccaac gactttaatt tttccggaat
    26881 caacgtaagg ttttaacact ttcatttgtc cggcgcggaa cagcttggcg ttgttatcta
    26941 ccggcgagcc gcccatcagg aagtaattac cttgcggaac aatatcgacc agggcttttg
    27001 cctgcagttc accgactttt tcgttatcga aagaaatata aaaatcgata tccgcatcgt
    27061 taatcatacg atcgtaagct aatactttaa taccttcttg tttggcttct tttacaacgt
    27121 tacttaatac ctgaccgtta tacggaataa tgacaagaac atcgacaccc cggtttatca
    27181 tgttttcaat ctgcgacatt tgtgtttctt cattgccatt tgcagactgt acaaatactt
    27241 tcgcgccgag agattctgcc tttttcacaa aaatatctcg atctttttgc cagcgttcaa
    27301 gacggagatc atcaatcgcc atacctattt tgacttcttt ggcgtgtgcc gcaacgttgg
    27361 taagcagaag tgaggtgcaa agggtgagta gaatgttctt tattttcatg gtgtagggcc
    27421 ttctgtagtt agaggacagc tttaataagt aacaatcacc gcgataaacg taaccaattt
    27481 ttagcaacta aacaggggaa aacaattaca gatttttatc tttcgattac gatttttggt
    27541 ttatttcttg atttatgacc gagatcttac ttttgttgcg caattgtact tattgcattt
    27601 ttctcttcga ggaattaccc agtttcatca ttccatttta ttttgcgagc gagcgcacac
    27661 ttgtgaatta tctcaatagc agtgtgaaat aacataattg agcaactgaa agggagtgcc
    27721 caatattacg acatcatcca tcacccgcgg cattacctga ttatggagtt caatatgcaa
    27781 gcctattttg accagctcga tcgcgttcgt tatgaaggct caaaatcctc aaacccgtta
    27841 gcattccgtc actacaatcc cgacgaactg gtgttgggca agcgtatgga agagcacttg
    27901 cgttttgccg cctgctactg gcacacattc tgctggaacg gggcggacat gtttggtgtg
    27961 ggggcgttta atcgtccgtg gcagcagcct ggtgaggcac tggcgttggc gaagcgtaaa
    28021 gcagatgtcg catttgagtt tttccacaag ttacatgtgc cattttattg cttccacgat
    28081 gtggatgttt cccctgaggg cgcgtcgtta aaagagtaca tcaataattt tgcgcaaatg
    28141 gttgatgtcc tggcaggcaa gcaagaagag agcggcgtga agctgctgtg gggaaccgct
    28201 aactgcttta caaaccctcg ctatggcgcg ggtgcggcga cgaacccaga tcctgaagtc
    28261 ttcagttggg cggcaacgca agttgttaca gcgatggaag caacccataa attgggcggt
    28321 gaaaactatg tcctgtgggg cggtcgtgaa ggttacgaaa cgctgttaaa taccgacttg
    28381 cgtcaggagc gtgaacagct gggccgcttt atgcagatgg tggttgagca taaacataaa
    28441 atcggtttcc agggcacgtt gcttatcgaa ccgaaaccgc aagaaccgac caaacatcaa
    28501 tatgattacg atgccgcgac ggtctatggc ttcctgaaac agtttggtct ggaaaaagag
    28561 attaaactga acattgaagc taaccacgcg acgctggcag gtcactcttt ccatcatgaa
    28621 attgccaccg ccattgcgct tggcctgttc ggttctgtcg acgccaaccg tggcgatgcg
    28681 caactgggct gggacaccga ccagttcccg aacagtgtgg aagagaatgc gctggtgatg
    28741 tatgaaattc tcaaagcagg cggtttcacc accggtggtc tgaacttcga tgccaaagta
    28801 cgtcgtcaaa gtactgataa atatgatctg ttttacggtc atatcggcgc gatggatacg
    28861 atggcactgg cgctgaaaat tgcagcgcgc atgattgaag atggcgagct ggataaacgc
    28921 atcgcgcagc gttattccgg ctggaatagc gaattgggcc agcaaatcct gaaaggccaa
    28981 atgtcactgg cagatttagc caaatatgct caggaacata atttgtctcc ggtgcatcag
    29041 agtggtcgcc aggagcaact ggaaaatctg gtaaatcatt atctgttcga caaataacgg
    29101 ctaactgtgc agtccgttgg cccggttatc ggtagcgata ccgggcattt ttttaaggaa
    29161 cgatcgatat gtatatcggg atagatcttg gcacctcggg cgtaaaagtt attttgctca
    29221 acgagcaggg tgaggtggtt gcttcgcaaa cggaaaagct gaccgtttcg cgcccgcatc
    29281 cactctggtc ggaacaagac ccggaacagt ggtggcaggc aactgatcgc gcaatgaaag
    29341 ctctgggcga tcagcattct ctgcaggacg ttaaagcatt gggtattgcc ggccagatgc
    29401 atggagcaac cttactggat gctcaacaac gggtattgcg ccctgccatt ttgtggaacg
    29461 acgggcgctg tgcgcaagag tgcactttgc tggaagcgag agttccgcaa tcacgagtga
    29521 ttaccggcaa cctgatgatg cccggattta ctgcgcctaa attgctatgg gttcagcggc
    29581 atgagccgga gatattccgt caaatcgaca aagtattatt accgaaagat tacttgcgtc
    29641 tgcgtatgac gggggagttt gccagcgata tgtctgacgc agctggcacc atgtggctgg
    29701 atgtcgcaaa gcgtgactgg agtgacgtca tgctgcaggc ttgcgactta tctcgtgacc
    29761 agatgcccgc attatacgaa ggcagcgaaa ttactggtgc tttgttacct gaagttgcga
    29821 aagcgtgggg tatggcgacg gtgccagttg ttgcaggcgg tggcgacaat gcagctggtg
    29881 cagttggtgt gggaatggtt gatgctaatc aggcaatgtt atcgctgggg acgtcggggg
    29941 tctattttgc tgtcagcgaa gggttcttaa gcaagccaga aagcgctgta cacagctttt
    30001 gccatgcgct accgcagcgt tggcatttaa tgtctgtaat gctgagtgca gcatcgtgtc
    30061 tggattgggt cgcgaaatta accggcctgt gcaatgtccc agctttaatc gctgcagctc
    30121 aacaggctga tgaaagtgcc gagccagttt ggtttctgcc ttatctttcc ggcgagcgta
    30181 caccacacaa taatccccag gcgaaggggg ttttctttgg tttgactcat caacatggcc
    30241 ccaatgaact ggcgcgagca gtgctggaag gcgtgggtta tgcgctggca gatggcatgg
    30301 atgtcgtgca tgcctgcggc attaaaccgc aaagtgttac gttgattggt ggcggggcgc
    30361 gtagtgagta ctggcgtcag atgctggcgg atatcagcgg tcagcagctc gattaccgta
    30421 cgggggggga tgtggggcca gcactgggcg cagcaaggct ggcgcagatt gcggcgaatc
    30481 cagagaaatc gctcattgag ttgttgccgc agctaccgtt agaacagtcg catctaccag
    30541 atgcgcagcg ttatgccgct tatcagccac gacgtgaaac gttccgtcgc ctctatcagc
    30601 aacttctgcc attaatggcg taaacgttat cccctgcctg accgggtggg ggataattca
    30661 catctatata tctcactaat taattaatat ttagtatgaa tttattctga aaatcatttg
    30721 ttaatggcat ttttcagttt tgtctttcgt tggttactcg taatgtatcg ctggtagata
    30781 tggagatcgt tatgaaaacc tcaaaaactg tggcaaaact attatttgtt gtcggggcgc
    30841 tggtttatct ggttgggcta tggatctcat gcccattgct aagtggaaaa ggctattttc
    30901 ttggcgtgtt aatgacagca acttttggta actatgcata tcttcgcgca gaaaaactcg
    30961 ggcaactgga taattttttt acccatatct gccagttagt tgcgttaatc actatcggtc
    31021 tcttgtttat cggtgtttta aatgcaccta tcaatgctta tgaaatggtg atctacccca
    31081 tcgccttttt tgtctgcttg tttggtcaaa tgcgtttgtt tcgctcggta tgagcaacat
    31141 aaagctctta cacattcagg aatgaaagga atactgtgat ggacaacaaa atatcaacct
    31201 attcaccggc ctttagtatt gtgtcatgga tagctctcgt tggtggtatc gttacctatc
    31261 tgttagggct atggaatgca gagatgcagt taaatgaaaa agggtattat tttgccgtac
    31321 tggtgttagg actgttttct gctgcgtctt atcaaaagac cgttcgggac aagtatgaag
    31381 gcataccgac cacttccatt tattatatga cctgcctgac tgtctttatt atctctgttg
    31441 cattactgat ggtaggtctg tggaatgcga cattattact cagtgaaaaa ggtttttatg
    31501 gactggcttt cttcttaagc ttgtttggtg cagtagcggt gcagaagaat attcgtgatg
    31561 ccggaataaa cccgccaaaa gaaacacagg ttacccagga agaatacagc gaataattca
    31621 cgtaagcccg gtcagtccaa tgtgaacggg cttttactta actcactaat ctgtttctgt
    31681 cgattcgttg taccagcata gaaagtaaca aactcgctgc caacgtcgcg caaaagatcc
    31741 aaataatatc cagtattggc caatttttaa gctcaattcc ccgggtgcgc agcgcatgga
    31801 taatcaaggc gtggaatccg tatataccca atgaatggcg ggagattaag ccaagtccgc
    31861 gaatggtacg cgtatccagc gtgtttttaa ccagagtcaa tagcgcgatt gcgcagataa
    31921 aaaccatcgg cccacagtaa agataccagg tatcggcaaa atttccgcgc cactgcaatt
    31981 catataatgt cccgcgagag ataataaaaa cccccgtcgc aaacagcgcg gcgctcaccc
    32041 acgacagtgc tttatgctgt gtgtccatca tccctatagc gcggcccaac atgccataca
    32101 gaatgtagta aaaagtatcg ccattgatat ataagttaat tggcagccat tcaaaaccgt
    32161 caattttctg cggcactgtg tttgggttag cgataatgcc aatcaccacc attagcacca
    32221 gcaacatttt tccgccgacg ttcttcacct gaatcagcgg tgaaaccaga taaatcaccg
    32281 caatcgcgaa gaaaaaccac aagtggtaaa acactggctt ttgcagcagg tttttcagcg
    32341 ctaactccat attgatggag gtaaacagcg caatgtagag cagtgcgatt gcgctataaa
    32401 aaatcagaca taagccgata cgcaagaaat ggcgcggctg ggcgctgcgt tcgccaaaaa
    32461 agagatagcc ggaaatcatg aaaaatagcg gcacgctgac acgagaggca gaattcagaa
    32521 cattggcgat atcccatgtg acggggctaa cactatgagc attggtcaca taccaggtag
    32581 tggtgtgaat catcaccacc attaaacacg ctatccctcg caggttatca atccagtaaa
    32641 ttttgggctg catctgtgtc tctgtatctg gttaaaaaaa gtctgaccga taaatcattt
    32701 ggttggcgca ctggaataat ctgagtttta tcactacagc ttatagaggc ttaaggaaat
    32761 tcgtaagata tcagccacta taccgatata aataataaga ctcacctgca aaccagacgg
    32821 taatttaatg atgatgaacg ctttctttcc ggcaatggcg cttatggtgc tagtgggttg
    32881 ttctacaccg tcacccgtgc agaaagcaca acgggtaaag gttgatcctc tgcgttcgtt
    32941 gaatatggaa gcgttatgca aggatcaggc ggcaaaacgt tataacaccg gcgagcaaaa
    33001 aatcgacgtc accgccttcg aacagttcca gggaagctat gaaatgcgcg gttatacctt
    33061 ccgtaaagag cagtttgtct gttcttttga cgcagatggc cattttttgc atctttccat
    33121 gcgttaagcc ctgctttttc ccgtttcgta ctgtatatct tccatccagc gggtatactg
    33181 atcccttcct ttaaatccac acgtatccag cacgaaataa tatgcaaaag tttgatacca
    33241 ggaccttcca gggcttgatc ctgaccttac aggattactg ggctcgccag ggctgcacca
    33301 ttgttcaacc attggacatg gaagtcggcg cgggaacctc tcacccaatg acctgtctgc
    33361 gcgcgctggg gccagaaccg atggcggctg cttatgttca gccttctcgt cgcccgaccg
    33421 atggtcgcta cggcgaaaac cccaaccgtt tacagcacta ctatcagttc caggtggtca
    33481 ttaagccatc gccggacaat attcaggagc tgtacctcgg ttctctgaaa gagctgggca
    33541 tggacccgac tattcacgac atccgtttcg tggaagataa ctgggaaaac ccgactctgg
    33601 gtgcctgggg actgggctgg gaagtgtggc taaacggcat ggaagtgacg cagttcactt
    33661 acttccagca ggttggtggt ctggagtgta aaccggttac cggcgagatc acctacggtc
    33721 tggaacgtct ggccatgtac attcagggcg tagacagcgt ttacgacctg gtctggagcg
    33781 acggcccgct gggtaaaacc acctacggcg acgtgttcca tcagaacgaa gtggagcagt
    33841 ccacttacaa cttcgaatac gcggatgtgg acttcctgtt cacctgcttt gagcagtacg
    33901 agaaagaagc gcagcagctg ctggcgctgg aaaatccgct gccgctgcca gcctacgagc
    33961 gtattctgaa agccgcccac agcttcaacc tgctggatgc gcgtaaagcc atctccgtca
    34021 ccgagcgtca gcgctacatt ctgcgcattc gcaccctgac caaagcagtg gcagaagcat
    34081 actacgcttc ccgtgaagcc ctcggcttcc cgatgtgcaa caaagataag taagaggcgg
    34141 ctatgtctga gaaaactttt ctggtggaaa tcggcactga agagctgcca ccaaaagcac
    34201 tgcgcagcct ggctgagtcc tttgctgcga attttactgc ggagctggat aacgctggcc
    34261 tcgcacacgg caccgttcaa tggtttgctg ctccgcgtcg tctggcgctg aaagtagcta
    34321 acctggcgga agcgcaaccg gatcgtgaaa tcgaaaaacg cggcccggcg attgcccagg
    34381 cgttcgacgc tgaaggcaaa ccgagcaaag cggcagaagg ttgggcgcgt ggttgcggta
    34441 ttaccgttga ccaggctgag cgtctgacta ccgataaagg cgaatggctg ctgtatcgcg
    34501 cccatgtgaa gggcgaaagc accgaagcac tgctgccgaa tatggttgcg acttctctgg
    34561 cgaaactgcc gatcccgaaa ctgatgcgtt ggggcgcaag cgacgtgcac ttcgtgcgtc
    34621 cggtgcacac cgtgaccctg ctgctgggcg acaaagtcat tccggcaacc attctgggca
    34681 ttcagtccga tcgcgtgatt cgcggccacc gctttatggg cgaaccggaa ttcaccatcg
    34741 acaacgccga tcagtatccg gaaattctgc gtgagcgcgg gaaagtcatc gccgattacg
    34801 aagaacgtaa agcgaagatt aaagccgatg ccgaagaagc agcgcgtaag attggcggta
    34861 acgctgactt aagcgaaagc ctgctggaag aagtggcttc gctggtggag tggccggtcg
    34921 ttctgaccgc aaaattcgaa gagaaattcc tcgcggtgcc ggctgaagcc ctggtttaca
    34981 ccatgaaagg tgaccagaaa tacttcccgg tgtatgcgaa cgatggcaaa ctgctgccga
    35041 acttcatctt cgttgccaac atcgaatcga aagatccgca gcaaattatc tctggtaacg
    35101 agaaagtcgt tcgtccacgt ctggcggatg ccgagttctt cttcaatacc gaccgtaaaa
    35161 aacgtctgga agataacctg ccgcgcctgc aaaccgtgct gttccagcaa cagctgggta
    35221 cgctgcgcga caaaactgac cgcatccagg cgctggctgg ctggattgct gaacagattg
    35281 gcgctgacgt taaccacgca acccgtgcgg gcctgctgtc taagtgtgac ctgatgacca
    35341 acatggtctt cgagttcacc gacacccagg gcgttatggg gatgcactat gcgcgtcacg
    35401 atggcgaagc ggaagatgtc gcggtggcgc tgaatgagca gtatcagccg cgctttgccg
    35461 gtgatgacct gccgtctaac ccggtagcct gtgcgctggc gattgctgac aagatggata
    35521 ctctggcggg tatcttcggt atcggccagc atccgaaagg cgacaaagac ccgtttgcgc
    35581 tgcgtcgtgc cgcacttggc gtgctgcgaa ttatcgttga gaagaacctc aaccttgatc
    35641 tgcaaacgct gaccgaagaa gcagtgcgtc tgtatggcga taagctgact aacgccaacg
    35701 tggttgatga tgttatcgac tttatgctcg gtcgcttccg cgcctggtat caggacgaag
    35761 gttacaccgt tgacaccatc caggcggtac tggcgcgtcg tccgactcgt ccggctgatt
    35821 tcgatgcccg catgaaagcg gtatcgcact tccgtaccct tgaagcagct gctgcactgg
    35881 cggcggcgaa caagcgtgtc tccaacattt tggcgaaatc tgacgaagtg ctgagcgacc
    35941 gcgtgaatgc ctctaccctg aaagagccgg aagaaattaa actggcgatg caggttgtgg
    36001 tgctacgtga caagctggag ccgtactttg cggaaggtcg ttaccaggat gcgctggtcg
    36061 aactggcaga gctgcgtgag ccggttgatg ctttcttcga taaagtgatg gtcatggttg
    36121 atgacaaaga attgcgtctc aaccgtctga ccatgctgga gaaactgcgc gaactgttcc
    36181 tgcgcgttgc ggatatttcg ctgttgcagt aataacgccg ttattaaaaa gcctgccatc
    36241 tggcaggctt tttttattat cgctaaataa tacagcaacc tttaataatc ttctgctgaa
    36301 taaagattat ctcatatatt aattttatga ggttttttta ggattatatc aaggagaaga
    36361 aacaaactta ttaagctaga atagccacgg gtgcttgaga ctgtttgtct caggtattca
    36421 ccgaaaggca gacagagaaa agccccacct gactataaat caaagtgagg ctaccctatg
    36481 cctgaacacc ataaggttag cctcttactc gttggaaatc aacacagggg gctgggaatg
    36541 ccgcagaaat atagattact ttctttaata gtgatttgtt tcacgctttt atttttcacc
    36601 tggatgataa gagattcact gtgtgaattg catattaaac aggggtgtta tgagctggcg
    36661 gcatttttag cctgcaattt aaaagagtaa gagtcttcgg cgggaagtta ttcccgcctt
    36721 tcttacggcg ttgcgcattc tcattgcacc caaatttatt tttcacaaaa ataatactag
    36781 attttattac gcgatcgatt atttatttcc tgaaaaacaa ataaaaaaat ccccgccaaa
    36841 tggcagggat cttagattct gtgcttttaa gcagagatta caggctggtt acgttaccag
    36901 ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac tttctgacct tcgtccagag
    36961 atttgtaacc atcgttctgg atagcagaga agtgtacgaa cacgtcttta gagccatcgt
    37021 caggagtgat gaagccgaag cctttgtcag cgttgaacca ttttacgata ccagtcattt
    37081 taccggacat agtgtattac ctttaataat taagtgtgcc tttcggcgat atggcgtgct
    37141 ttacagattt tgaagcgtta aaggaatgtg cactacgagg ggtatcaacg ataactcttg
    37201 aagggacttg ccttactaca ctgctttaat ggtctgtacg tcaaaccgtt gatgtgcatt
    37261 aagccacgca ttggcgggtg atgcaacaat tatttttcat atttatgatt aatcggttgg
    37321 aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt ggataaaagt ctattccatc
    37381 aactgcttac ttaatgccgg gttggcttga atcaaacgca tcaattttag ttcggcactt
    37441 gaaggcttca cgcgtctgga ttcccattcc tttaccatgg cgactgatac gcccaaaacc
    37501 cgggcgaaat catcgatttt taatcctgtc ccttttcgta actgctcaat ttcggtacag
    37561 gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat ctttaaaaac aatctgttcc
    37621 aggctgctca acagctcatg cattggatct ttatattcca ttgaaaactc ctcaaatcac
    37681 actgcgggat cgtgaattgc atcgaagctc attaagaata gtcgggaaaa gcagacacag
    37741 cggggtgaag tcagggcatg ctctggctat gattcgattc agccagtcat ttagcgtgac
    37801 ttatagccac ctaacgatct gataaatgcc gatatatctc ttaccactcc tggtttgtaa
    37861 agatttcgaa aatctaacct tctgcgaagg cgtaaatctc tggctacaaa aaaagcaacg
    37921 gcactatcat tttttaacaa tgaaacaaca aattaacctt gtttcatttt ctccatgatt
    37981 ttaccaggaa gattacgtag aaagggtatc ctgcaagcct gtcctggact atccttgtca
    38041 cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg aaaggagtaa gaaaatggcg
    38101 acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg cgttattaat ggtagttagc
    38161 ctgagtgggt gttttgataa agaaggcgat cagcgtaaag cgtttatcga cttcctgcag
    38221 aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga ctgccgatca gaaaaaacag
    38281 tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt attctcagca ggtgaatcag
    38341 gcgatggatt ccggtctgcg cccggtcgta gacagcgtta acgcaattcg cgtaccgcag
    38401 gattacgtta cgcaaagcgg tccgctgcgt gaaatgaacg gttcgctggg tgtgctggcg
    38461 caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc actctgcgct aaaacagacc
    38521 gatgatttga aaccggtctt tgatcaggca ttcaccaaag tggtaacgac gccagctgat
    38581 gcattgcaac cgttgattcc ggctgcacaa actttcacgc aacaactggt gatggttgga
    38641 gactacattg ctcagcaagg tactcaggtg agctttgtcg caaatggcat ccagttcccg
    38701 acttcacagc aggcaagtga atataacaaa ctgattgcgc cattaccggc acaacatcag
    38761 gcgtttaatc aggcctggac tacagcagtt actgcaaccc aataaagagt aaaagcccga
    38821 gcgattatcg ggctttactt cacgcagtcg cggcttagtc cgcgacgtgc ggattcacac
    38881 agttcttctc aacctttcct tgtaacgcat caatcaaatt atccacggca caggcggcca
    38941 tgccataacg cgtctcatgg gtggcagatc caatatgcgg tactgcgacg acgttggcca
    39001 ttgagagcaa cggcgaatct acggacagtg gctcttgttc gaagacatcc agcccggcgg
    39061 cgtgaatttc gcctttctgc aatgcagcaa tcagtgcatt ttcatcaacc accgggccac
    39121 gtccggcatt aatgaaaatg gcggaggatt tcattttggc gaattgttct gcgccaaaca
    39181 gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac gaaatctgac tcttgtaaca
    39241 gagtatccaa atcgcagtag cgggcgttga agcgttcttc tgcttcttta tggtggcggc
    39301 gcgcgttata gaggatgggc atgttgaagc caaagtgcgc acgttgtgcc agcgccatac
    39361 cgatccgtcc catcccgaca atgcccagtg ttttatggtg aacgtcagtg ccgtaccagt
    39421 ccgggcctat gctcgcggtc cattcgcctg cttttacccg ttctgctacc tccacaaccc
    39481 gacgagcggt agacaacacc agcgccatca gcgtatcggc gacggtttct gttaatacgg
    39541 ttggcgtgtg catcagcaga atttttcggg cggtaagcgc atcgacatca aaattgtcat
    39601 agccgacgga gatcgttgat gtggcacgca gtttcggcat tttttccagc aatgcggcat
    39661 caacattctc gtttgaaccc agtaaacctt cagcttcggc aaaaattgct gcattttgtt
    39721 cgacggtttg tgggctgagg tttgccacct ggtgaacggt gaaatgctct tgcaggcgtt
    39781 gcagtaaatc atcaggtaag gctttgtaga ggataacgga cggcttcatg cttctctcca
    39841 ttcactgata aggcgatcac cttagcctga aatatgatga aggaaaactg aagaatgcca
    39901 ggtgatttct gcatcacctg gcatgaaagg gattacagcg ggcttaaggt aatttctaca
    39961 cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg cgattgggtt agccgggcca
    40021 aggccctgag tacggatgcg gctggcgtcc acgccctggg tgatcaacgc gctggcaacg
    40081 gaatccgcac gttgctggga gagacgcatg ttcaggtcgt gaccacccgt gctgtcggta
    40141 taaccaatca cgttaaccgc cgttttcgga tactctttca gcaccattgc cacgccggtc
    40201 agggtgttag cgcccgcagg tttcagggtc gcgctgctgc tgtcgaaggt cacattgttc
    40261 ggcatattga ggataatgtt atccccgctg cgggttacgc taacaccagt gccgcgcatt
    40321 ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac ccacgccgcc gcccagagct
    40381 gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat ctttcttcga agaagagagc
    40441 gcaccaatac ccgcgcccac gagagagccc agacctgcgc cgatagcaga tttacctgct
    40501 tcgcgttcgc cggtgtaagg gttagttgtg cagccagata ccgccagagc gccactcact
    40561 acggcggcaa taagataaac acgtttcttc attgttaatc cttaataacc tttttattct
    40621 ttgccacggg ttccgtggcg ggagattatg ccgcgtgaac atgaagatta ttcctgggaa
    40681 tactcggaaa tttgtaagta atatttaact gctcaataca tctaaccttt caggagtttt
    40741 cggtttggcc aactcatcct cacgatattc cgttctgact gccgcccatt gggggcccat
    40801 gctggttgaa accgacggcg aaaccgtgtt tagctcgcat ggcgcgttag ccacaggaat
    40861 ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac agcaatacac gggtacgatt
    40921 tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca ttcgtgggca
    40981 ggatgaattt gttcgcgtga gttgggatga ggcgctggat cttattcacc aacaacataa
    41041 acgcattcgt gaggcttatg gtccggcatc gatttttgct ggttcctacg gctggcgttc
    41101 aaacggcgtg ctgcataagg cctcgacatt attacaacgc tatatggcgc tggcaggcgg
    41161 ttatatcggg catctggggg attattcgac cggtgcggca caggcgatca tgccgtatgt
    41221 cgtgggtggc agcgaagttt atcaacagca aaccagttgg ccgctggtgc tggaacatag
    41281 cgatgtcgtg gtgttgtgga gtgctaaccc actcaatacg ctgaaaattg cgtggaatgc
    41341 atctgatgag caggggcttt cttacttttc cgcactgcgt gacagcggga aaaagctgat
    41401 ctgtattgat ccaatgcgat cggaaaccgt cgatttcttt ggcgataaaa tggagtgggt
    41461 ggcaccgcac atgggcaccg atgttgcgct gatgctgggg atcgcccata cgctggtgga
    41521 aaatggttgg cacgacgaag cgtttctgac gcgttgcacc acaggttatg ccgtcttcgc
    41581 ctcttatttg ctgggcgaga gtgacggaat agcgaaaaac gccgaatggg cggcagagat
    41641 ttgtggggtt aacgcagcga aaatccgcga gctggcggct cttttccacc aaaacacgac
    41701 catgctgatg gctggctggg gaatgcagcg ccaacagttt ggtgagcaaa aacactggat
    41761 gatcgtcacg ctggcggcaa tgttggggca aatcggcaca cccggcggcg gttttggtct
    41821 ttcttatcat tttgccaatg gtggtaaccc cacgcggcgt tctgcggtgc tctcttccat
    41881 gcagggcagc ttgccgggtg gctgcgatgc ggtggataaa atccctgttg cccgcattgt
    41941 tgaagcactg gaaaaccctg gtggcgcata tcaacacaac ggtatgaacc gacatttccc
    42001 ggatattcgt tttatctggt gggcgggcgg tgccaacttt actcatcatc aggataccaa
    42061 tcgtctgatc cgtgcctggc aaaaaccgga gctggtggtg atctctgaat gcttctggac
    42121 ggcggcggcg aaacacgcgg atatcgttct gcctgcgact acctcttttg agcgtaatga
    42181 tctcaccatg accggtgatt acagtaatca gcatctggtg ccgatgaagc aagtggtgcc
    42241 gccacgctat gaagcgcgta atgattttga tgtttttgcc gagttgagtg aacgttggga
    42301 gaagggcggc tatgcgcggt ttacggaagg aaaaagtcag ctgcaatggc tggaaacgtt
    42361 ttataacgtt gcccggcagc gcggggcaag ccagcaggtt gaattgccgc catttgctga
    42421 gttctggcaa gccaaccagt taattgagat gccggaaaac ccggacagcg agcggtttat
    42481 tcgcttcgct gatttttgcc gcgatccgct ggcgcatccg ttaaaaaccg ccagcggcaa
    42541 gattgaaatc ttctcacagc gtattgccga ttacggttac ccggattgcc ctgggcatcc
    42601 aatgtggctg gagccggacg aatggcaggg caatgccgaa ccagaacagt tgcaggtact
    42661 ttctgcccat ccggcgcacc gcctgcacag ccagctgaat tacagttctc tgcgcgaatt
    42721 gtacgcggta gcaaaccgtg agcctgtcac cattcatcct gacgatgctc aggcgcgcgg
    42781 cataaccgaa ggggatatgg tgcgggtgtg gaactcccgt gggcagatcc ttgccggagc
    42841 ggtcattagc gagggaatta aacctggcgt gatttgcatt cacgaagggg catggccgga
    42901 tctggattta accgctgacg gtatttgtaa aaacggcgca gtgaacgtgc tgaccaaaga
    42961 tctccccagc tcgcggctgg ggaatggctg tgcgggtaat acggcgctgg catggctgga
    43021 aaaatacaac ggtccggaac tgacacttac agcgtttgaa ccaccggcca gctcataatc
    43081 caggtgggta gttgggtttc atcctgccat gcgcaatcga caatgtgaaa accctgcgca
    43141 cggtaaaaat ctatcgccgg ttgatttttt tgataaacct ccagcatcag atggggatag
    43201 cgctgctgca catactgcat cagcgcctta ccaataccgc gtctgacggc cttcggtgcg
    43261 acaaacattg ctgccagaaa tcggccttcc ataatgctga caaaaccgag aagcttaccg
    43321 tcctcttccc agacccagtt ttgcgcgttg gcaagatagg catcccgcac cagcggaatg
    43381 cagtcacgcc agtaattcgc ttttataaag ggatgccccc aggttgtact ttccagccac
    43441 agttcgagga tcgcggggag ttctgaacgt tgcgcttccc gaatcatggt ttatttcccg
    43501 gatagcaaca gcagccaacc acatgatcat tcaccagccc acatgcctgc ataaaggagt
    43561 aacagattgt ggtgccgaca aacttaaaac cacgtttttt cagtgcctta gatagggcgt
    43621 cggaggcgga cgtagatgtg ggaatttcgc tcaacgttgt ggcttgtgtc acctgtggct
    43681 gatgatttac aaacgaccag acaaagtcgg caaacggttc gccgttctgt tccatttgca
    43741 ggtacgcccg cgcattacca ataattgcct gaattttccc tcgatggcgg ataatcccgg
    43801 cgtcctgtac cagtctttcg acatcctctt cctgcattgc tgcgaccttc accggatcga
    43861 actgatgaaa gcaggcgcga tagttttcgc gttttttgag gacggtgatc cacgataacc
    43921 cagcctgctg cccttcaagg cagatcattt cgaacagttt tttactgtca gtttcaggca
    43981 cgccccattc attatcatgg taggcaatat aaagcgggtc ctgactcacc cagccgcaac
    44041 gttccatact ttccctcgct atgtacccga tcgtaaaaat ttcagccgct tttcttgacg
    44101 catcggtgaa aaagtcaata tttaatacag ggctactatg cccgatatcc ttcatacaat
    44161 gacaataata ttgccgaatt cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa
    44221 gcggtggtcg ctggcagcta agcctgctgg cgagcgtggt aatcagtgcc ttttttctca
    44281 acacagctta cgcctggcaa caagaatata tcgttgatac gcaacccgga cattccacag
    44341 agcgttacac ctgggatagt gatcatcaac ctgattacaa cgatattttg tcgcaacgta
    44401 ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct ggcggaagaa actcctgtgg
    44461 atgtgaccag cagtatgagt atgggctgga attttccttt atatgaacag gttacaaccg
    44521 gcccggtcgc ggcattacat tacgatggca caaccacctc gatgtataac gagtttggcg
    44581 acagtactac cacgctgacc gatccgttat ggcatgccag cgtgagtagc ttaggctggc
    44641 gtgttgactc ccggcttggc gatctccgac cctgggcgca aatcagctat aaccagcaat
    44701 ttggcgagaa tatctggaag gcgcaatcag gcctgagccg gatgacggcg acaaaccaga
    44761 acggcaactg gctggatgtc accgtaggcg ctgatatgtt gctcaatcaa aatattgccg
    44821 cctatgccgc gctaactcag gcagaaaata ccactaataa tagcgactat ctgtatacga
    44881 tgggggttag cgccagattt taacgtaaca gtcacaattg aaaccattga ataacaacag
    44941 ttgtggcgat agtgggtgct aacttaccaa ataataaatt tggtgaataa ttgtcgcgtc
    45001 attcattcct gaactaaggc atttcattcc gttctgatgg catttcatgc cgtttttccc
    45061 caggcataaa gtgcacttcg ttatggttgt cggcagagat ttttcctttt tattactgca
    45121 ggaatactgc catgacacct tcaaattatc agcgtacccg ctggctgaca ctcatcggta
    45181 caatcattac ccagtttgcg ctggggtcgg tttatacctg gagcctgttt aatggcgcgc
    45241 tttccgccaa gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc ttgttaagtc
    45301 tggggctggc aatttcatct tctgttgcgg gcaaattaca ggaacgtttt ggcgttaaac
    45361 gcgtcaccat ggcttccggc attttgctgg gattaggctt cttcctgacc gcgcattctg
    45421 acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg
    45481 gttacctgct gacgctctct aactgcgtga agtggttccc ggagcgtaaa ggtctgatat
    45541 ccgcgttcgc tatcggttct tatggtctgg gtagcctggg tttcaaattt atcgacacgc
    45601 ggctgctgga aacggtgggt ctggaaaaaa cctttgtgat ttggggagcg attgcgctgg
    45661 tgatgattgt tttcggcgca acgttaatga aagacgcacc aaaacaggaa gtgaaaacca
    45721 gcaatggtgt ggtggagaaa gattacacgc tggcagagtc gatgcgtaaa ccgcagtact
    45781 ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg cctgtacgtg attggggtag
    45841 cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt ttccgcagct aatgcagtca
    45901 ctgttatttc catcgccaac ctttcaggtc gtctggttct ggggatactg tctgacaaaa
    45961 tcgcccgtat ccgtgttatc accattggtc aggtgatttc gctagtgggt atggcggccc
    46021 tgctgtttgc accactgaat gcagtgacgt tctttgcagc gattgcctgc gtggcattta
    46081 actttggcgg cactattacc gtctttccgt cactggtcag tgagttcttc ggcctcaata
    46141 acctggcgaa aaactacggt gtgatttacc tcggtttcgg tatcggtagc atttttggtt
    46201 cgattatcgc ctcactgttt ggcggtttct atgtgacctt ctacgtgatt ttcgccctgc
    46261 tgattctgtc attggcgctt tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg
    46321 aggcgcatgg ctccctttaa tgctcagccg cgagctactg ggcttcagct tccgtgtgca
    46381 tcaaacgcat caacgaaatg tataaacaat gactcgtaaa atttgggcca gttaactggc
    46441 cctttttact gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt
    46501 gtgcttcatg cacactcttt ccccacactt tttcgctttg ctgtggtcta cttattcgcg
    46561 cgtgtagatt ttacttatct gactacctcc gcacttttcc cctgccgggc ctgaaaagcc
    46621 gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc
    46681 gattacacag cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg
    46741 cgcggttttt tatcgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt
    46801 ttctgctgtt gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct
    46861 atcgctgttt ggccgccgca gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc
    46921 aggtgccagc tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc
    46981 cgtcatgacc accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg
    47041 gttaatcgcc gttagtgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac
    47101 cttgctccga caactgcgaa ccccagggca gcgtattcgc agcctggcgg tcgtcgtact
    47161 ggcgggtatt atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga
    47221 gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc
    47281 atcaaactgg ctttctgcgt tggggctgta tgcctgggcg cgggtcgatg aatcttccga
    47341 taataattca ttgcttaatc cggcgaagaa attcacttat caggcaccgc aaaacgttga
    47401 tgacacttat gtcgtgttta tcatcggtga aaccacgcgt tgggaccata tgggcatttt
    47461 cggctatgag cgtaatacca cgccgaaact ggcccaggag aaaaatctgg cggcgttccg
    47521 tggttactcc tgtgataccg caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg
    47581 gggcgcggaa gataatccgc agcgcacatt aaaagaacag aacattttcg cggttctgaa
    47641 gcagttagga ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa
    47701 cacgatggcg gacaacattg cttatcgtga gcagattggt gcggagccac gtaatcgtgg
    47761 caagccggta gatgatatgt tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc
    47821 ggatggtaag catctgatca ttctgcatac caaaggttcg cattttaact acacccagcg
    47881 ttatccgcgt agcttcgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac
    47941 caaagcgcag atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc
    48001 cagcgtgatt gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg
    48061 tgagtcaatt aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga
    48121 gcagttccgc gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggccaa
    48181 tgctcaggca tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcatgt
    48241 agagctgtac gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa
    48301 cgaaaacaac aactggtgtc acatcccgca gacaaaagtg gcagcggcta actaagcggc
    48361 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat
    48421 ctgcgcagta agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt
    48481 cgggacgaag gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct
    48541 cagcgagcag gcttttttgc atttgaagcc catgaggatg agaacctccg ggggcgggga
    48601 ggttcgactc gagcgaagcg agagaacgtt gcgccagcaa cggcccgcag ggcgagccac
    48661 gaagtggcga gtaatcctct atcaccgacc aaattcgaaa agcctgctca acgagcaggc
    48721 ttttttgcat ttgtagctca tgaggatgag aacctccggg ggcggggagg ttcgactcga
    48781 cgtaggcctg ataagacgcg tcagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg
    48841 atgcggcgtg aacgccttat tcgacctaca cgccacacat ttccctgcca aatttgcgcc
    48901 ccatatctca ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg
    48961 cttaaatctt tttttgcgtt acttatggct gaccctggca ttttccgctg tgcgttttag
    49021 cgttcatggt attaatcgct cagatatcca ccttttcctc agattttttt cattaaatgc
    49081 ataaaactta atcaccaaat gttgctaaat attaagctga ttgttctggt catgttggag
    49141 tagcacaaat ttctctgctg caaatagctg tttgaagttt ttttaatctt tgtttgtgga
    49201 ttctcaccgt tggtgtaaat cccggttggc tgtattgacg ttttcacatt ctgttgacag
    49261 attgtaggtc acgaggggca ttttatggag gatccgcact gttacactga tgttaattag
    49321 tacggcatcc ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca
    49381 gcgacaaccc actgcaaagg gttaaaacaa caaacatcac aattggagca gaataatgcg
    49441 tatttccttg aaaaagtcag ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt
    49501 cgcagcaagt gttcaggcta aaactctggt ttattgctca gaaggatctc cggaagggtt
    49561 taacccgcag ctgtttacct ccggcaccac ctatgacgcc tcttccgtac cgctttataa
    49621 ccgcctggtt gaatttaaaa tcggcaccac tgaagtgatc ccgggcctcg ctgaaaagtg
    49681 ggaagtcagc gaagacggta aaacctatac cttccatctg cgtaaaggtg tgaagtggca
    49741 tgacaataaa gaattcaaac cgacgcgcga actgaacgcc gatgacgtgg tgttctcgtt
    49801 cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt tctggcggta gctacgaata
    49861 cttcgaaggc atgggcttgc cggagctgat cagcgaagtg aaaaaggtgg acgacaacac
    49921 agttcagttc gtgctgactc gcccggaagc gccgttcctc gctgatctgg caatggactt
    49981 cgcctctatt ctgtcaaaag aatatgctga tgcgatgatg aaagccggta caccggaaaa
    50041 actggacctc aacccaatcg gaaccggtcc attccagtta cagcagtacc aaaaagattc
    50101 ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga tcgatacgct
    50161 ggtcttctct attacccctg atgcttccgt gcgttacgcg aaattgcaga agaatgaatg
    50221 ccaggtgatg ccgtacccga acccggcaga tatcgctcgc atgaagcagg ataaatccat
    50281 caacctgatg gaaatgccgg ggctgaacgt cggctacctc tcgtataacg tgcagaaaaa
    50341 accgctggat gacgtgaaag ttcgccaggc actgacctac gcggtgaaca aagacgcgat
    50401 catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat
    50461 gtggggctat aacgacgacg ttcaggatta cacctacgat cctgaaaaag cgaaagcctt
    50521 gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca
    50581 acgtccgtat aacccgaacg ctcgccgcat ggcggagatg attcaggcag actgggcgaa
    50641 agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt gagtacctca agcgtgcgaa
    50701 agatggcgaa catcagacgg taatgatggg ctggactggc gataacgggg acccggataa
    50761 cttcttcgcc accctgttca gctgcgccgc ctctgaacaa ggttctaact actcaaaatg
    50821 gtgctacaaa ccgtttgaag atctgattca accggcgcgt gctaccgacg accataacaa
    50881 acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac gatcaggctc cggcactgat
    50941 catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa gtcaaaggct atgtggttga
    51001 tccattaggc aaacatcact tcgaaaacgt ctctatcgaa taattaaaag ccatacaaga
    51061 ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat caggcctacg aaaattctgc
    51121 aatgtattga atttgcacga ttttgtaggc cggataaggc gttaacgccg catccggcat
    51181 aaacaaagcg cactttgtca acaatctgta tacccggtgg cgctgtgcct ctgcgtgcag
    51241 cgccatccgg cagcaatact tcattctctg tccgacaagg gcgggaattg atttgtgagc
    51301 aatacagaca cgcagttcca ggctgcgggt cactatagag aatccgggtt atgttgcagt
    51361 ttattctccg acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat
    51421 ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat catggcaggc gaacgtggga
    51481 tctcccctga gcgtcacgcg cagctgctgg ctgaactcgg cttagataaa ccgatgtggc
    51541 agcagtatct ccattacatt tggggcgtaa tgcacggcga tctaggcatt tcaatgaaaa
    51601 gccgaattcc agtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg
    51661 tctgcgcgat gatttttgct acggcagtcg gtattccggt tggtgtgctg gctgcggtta
    51721 aacgcggttc cattttcgat cacacagcgg ttggcctggc gctgaccggc tactcgatgc
    51781 cgatcttctg gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacgc
    51841 ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga ctccaatccg ttaaccggtt
    51901 ttatgctaat cgacaccgcc atctggggtg aagacggcaa ctttatcgat gccgtcgccc
    51961 atatgatctt gcctgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga
    52021 cacgctcctc gatgctggaa gtgctgggcg aggattacat ccgcaccgcg cgcgccaaag
    52081 ggctaacccg catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtgg
    52141 tgaccgttat cggcctgcag gtgggaacat tgctggcggg ggcgattctg accgaaacca
    52201 tcttctcgtg gcccggtctg ggacgctggt tgattgacgc actgcaacgc cgcgactatc
    52261 cggtagtgca gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacttgctgg
    52321 tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggacatca
    52381 tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt gccgatgacc ccgttacagg
    52441 agttctggca ctattttaaa cgcaacaaag gtgcggtcgt cgggctggtt tacgtcgtca
    52501 tcgtgctgtt catcgcgatc tttgccaact ggattgcacc ctataacccg gcggaacagt
    52561 tccgcgatgc actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc
    52621 tgggcaccga tgacgtaggc cgtgatgtgc tgtcgcgcct gatgtacggt gcgcgcctgt
    52681 cgctgctggt tggctgtctg gtagttgtgt tatcgctgat tatgggcgtt attctcggcc
    52741 tgatcgccgg ttactttggc ggcctggtcg ataacatcat tatgcgcgtg gtcgatatca
    52801 tgctggcgct gccaagtctg ctgctggcgc tggtgctggt ggcaattttc ggcccgtcga
    52861 ttggtaacgc cgcgctggca ctgaccttcg ttgccttgcc gcactatgtg cgcttaaccc
    52921 gcgccgccgt gctggtggaa gttaaccgcg attacgtcac cgcgtctcgc gtggcgggtg
    52981 ccggggcgat gcgtcagatg tttattaaca tcttcccgaa ctgccttgcg ccgctgattg
    53041 ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg
    53101 gcatgggggc acagccgcca acgcctgagt ggggcaccat gctctccgac gtgttgcagt
    53161 tcgcgcaaag cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc
    53221 tggcatttaa cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt
    53281 aagaggttcg agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa
    53341 agcgcgccgt tccgcgccgt agaccgcatc agctacagcg taaaacaggg cgaagtggtc
    53401 gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt cactggcgat tatggggctg
    53461 attgattatc cgggccgcgt aatggcagaa aaactggagt ttaacggcca ggatttgcag
    53521 cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag
    53581 gacccgatga ccagccttaa cccgtgctac accgtgggtt tccagattat ggaagcgatt
    53641 aaggtgcatc agggcggcaa caaaagtacc cgccgtcagc gagcgattga cctgctgaat
    53701 caggtcggta ttcccgatcc ggcatcgcgt ctggatgttt acccgcatca gctttccggc
    53761 ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct gtcggccaaa actgctgatt
    53821 gccgatgaac caaccactgc gctggacgtg accattcagg cgcaaatcat cgaactactg
    53881 ctggagctac agcagaaaga gaacatggcg ctggtgttaa ttacccatga cctggcgctg
    53941 gtggcggaag cggcacataa aatcatcgtg atgtatgcag gccaggtggt ggaaaccggt
    54001 gatgcgcacg ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg
    54061 ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt tgccaggtgt cgttcccggc
    54121 aagtacgacc gcccgaacgg ctgcctgctt aacccgcgct gcccctatgc cactgacaga
    54181 tgtcgcgctg aagaaccggc gctgaatatg ctcgctgacg ggcgtcagtc caaatgccat
    54241 tacccacttg atgatgccgg gaggccgaca ctatgagtac gcaagaggcc accctgcaac
    54301 aaccgctgtt gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggcatgttcg
    54361 cgccggaacg tctggttaaa gcgctggatg gcgtttcgtt taaccttgaa cgtggcaaaa
    54421 cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga
    54481 tgatcgaaac gcccaccggt ggtgagctgt attaccaggg gcaggatctg ctcaagcacg
    54541 atccgcaggc gcagaagctg cgtcggcaga aaatccagat tgtcttccag aacccttacg
    54601 gttcgctgaa tccgcgtaaa aaagttgggc aaattctcga agagccgctg cttattaaca
    54661 ccagcttaag caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc
    54721 tgaaaaccga gcactatgac cgctatccgc atatgttctc cggcggacaa cgtcagcgta
    54781 tcgctattgc ccgtggtctg atgctcgacc cggatgtggt gattgccgat gagccggttt
    54841 ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct gatgatggat ttgcagcagg
    54901 aactggggct ttcttatgtc tttatctcgc acgacctgtc ggttgtggag cacattgccg
    54961 atgaagtgat ggtgatgtac ctgggccgct gcgtggagaa ggggacaaag gaccaaatct
    55021 tcaataaccc gcgtcatcct tacactcagg cgctgctctc cgcgacgccg cgcctgaacc
    55081 cggacgatcg ccgcgagcgc atcaagctca ccggtgaact gccaagcccg ctcaatccac
    55141 cgccgggttg cgccttcaac gcccgctgtc gtcgacgctt cggcccctgc acccagttgc
    55201 agccagcact gaaagattac ggcggtcaac tggtggcctg ctttgccgtt gatcaggatg
    55261 aaaatccgca gcggtaatgt tcaaaaccgg agccttgtgc tccggtttac tcaatcagct
    55321 tcaacagtgg cgacaagcat agcaaaatgc cgtacagcag aatcagccag gctttaaatc
    55381 cgcgaagttt ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca
    55441 caatgccata taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa
    55501 cccaaatcgt caacgtaatg acgataaaag cgcagatcgc cagagtcagc atgcgtgagt
    55561 taattttctt ggtatcaata attcggctta acagattgag aataatgcct ttaatagcct
    55621 cgtggaaacc gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg
    55681 tagaggtgat atgaatgata tgtccaggga tcacctgcgc ggccagcgcc agtgccgaga
    55741 tattttgttc aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg
    55801 caaaaaacag gatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga
    55861 gccgggttgc cagtacttta tctgcttccc gtttacgata ggcaatattc attgggttaa
    55921 gtacctgaat aaataccgca gaaaagaagc aaaatggaat ggtaagcagg acatcgcgga
    55981 aaaagacgga ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg
    56041 caaaaccgaa cacgacaata atccctactt tgaccaccac cattggccca gaaatcttaa
    56101 acagtaatcg ttcaccacca gacgcaatcg ccaccagtac ggcgaaaata gcgactttat
    56161 aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc
    56221 tgtcgaaaac cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga
    56281 taaccccgag gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt
    56341 cattacagga atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg
    56401 caggataagc aatgatcgct gcggtaataa atacccaaat tcccttcaag ccaatttgta
    56461 ctggcatcag cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga
    56521 agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga tagtgtgttg tgctgcataa
    56581 agtcctgtcc agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca
    56641 acaggtggag aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag
    56701 gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc
    56761 ggatatgaac tgcaactatt gcaaataaag aaattaagct ggaaggcatc gcgcaatgag
    56821 gaagattgcc gggtgctgac agtaaaaagc aatcgtaaaa tcagcggcaa gccggaaggg
    56881 ttccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggagaga
    56941 gaaaaccccc gcacgttgca ggtatgtacc tgacaacata acgggggcta atcttgaacc
    57001 tagaccactc aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc
    57061 tcgcagagct gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc
    57121 ttgccagtat gatcgtgaac tggctgaaca agcggaagta acgtgtcatg cgggcgtcag
    57181 gctgccgtaa tggcaatttg cgcccggacc aggccgcagg ggtgaaactc tgcggccttt
    57241 ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat
    57301 cctggtattg aataactact gcatttgagt tctcggagac cggtgctgtt tgtggcaacc
    57361 cactggtgag ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc
    57421 gaaccaccag atcggagata gccaggaagc tgctcggttg gtcaatgaca atcggtgccc
    57481 cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac ggggacgtcg gtgatagacg
    57541 ggctagggat atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt
    57601 gttccggcac cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg
    57661 cgtccagttc atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgaccc
    57721 ccggataatg gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctat
    57781 ttttatcttt ttcggtaacg tccagccagc ggttaagcac ggcggtatcg tcataaaccg
    57841 gcgaaccatc aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct
    57901 gcatgccgcc attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgcccca
    57961 tcatcaggtg ctgggtaaag cccagtttcg aaaggttatc aaacagatag cagtcgttat
    58021 ttgccggttg atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga
    58081 tcgccgccgg gccactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat
    58141 gcgaccacag tggatgcgac atcaacccgg cggcttctat atccgaccag gaaagcgaac
    58201 agatgttaat caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg
    58261 tcgatttacg tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg
    58321 ttgttggcgg tgcagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgccg
    58381 caacggttgc cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgtccggctg
    58441 gccacaagga gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa
    58501 ccacaaaaac ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa
    58561 aaatggcccc aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg
    58621 tactgaaccc cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccagg
    58681 tgtcatgcca gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca
    58741 ggctgtagcg cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcggat
    58801 ggaagttaag atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc
    58861 cggaaaggcc gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgtgtaaatt
    58921 gagtcatttt ttggttgccc tggctttttc cgtgcggcgt aacgtcccgg ccggtttaac
    58981 ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg
    59041 cgccagatag cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc
    59101 gctgatggtc atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt
    59161 attcgtcgcc agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt
    59221 aaaggcagcg gcatgcccca ttgttctggg gcaagcaagc gcatctgcac cagctcggca
    59281 ctgatttgat catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat
    59341 gggaaaatat gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc
    59401 accagccgat taccgccaat ggtcatgata tcgccggtac ggttagggcg acacagcgtc
    59461 agggcttgtt caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg
    59521 tgggcaggta atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac
    59581 ttctggaaac cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc
    59641 ggcacatagc gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag
    59701 agtggcgcat tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg
    59761 gtggcgcgca ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg
    59821 cgacgcaggg tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa
    59881 aagaccaccg tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg
    59941 aacagttgcc agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga
    60001 atgcgttttt cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag
    60061 ataccatttt gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac
    60121 cagaaggcaa tatccagcaa atgttggtca ccctgaaaac gcaaactggc aagaccaaaa
    60181 agtgaacggt attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta
    60241 attaccaaaa gcgaacaatg gtttaacctg ctccatttat tcattttatc caaccatgag
    60301 cgaagccgct cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg
    60361 taattatggg gatcaataga acactgcaaa tcacgggtca aatagtatag acctttttca
    60421 tgatttagca ttgaaaataa ttttattttt tccggccctt gagaatcatc taattgaaag
    60481 attttcgccg gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat
    60541 gcaattgttt gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag
    60601 acgccgcctg ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat
    60661 acagggtcca caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa
    60721 gctaagaaat aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt
    60781 tagaaggtca caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa
    60841 cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc
    60901 tcacggttag aattgtctca tgaacggtac ggttatttca tagggatcaa gcaaaatgaa
    60961 taacaatgaa ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat
    61021 tgtggcgtta aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg
    61081 cgaacagttg gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa
    61141 gtaggggatt ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca
    61201 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgttctggtg
    61261 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt
    61321 cagggctggg ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc
    61381 tgccttttgg tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc
    61441 tgagcgatat ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt
    61501 taatcgactt accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc
    61561 actcgctggc aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc
    61621 tgccggatgg cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg
    61681 atatttacca gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg
    61741 atgaagcgat ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg
    61801 cgctggcggc tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc
    61861 tgaaaacgcc agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc
    61921 ggtcaacgcg cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt
    61981 cagcgcgacg ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca
    62041 cccgcgctgg cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc
    62101 gcgtccgcgt ccgctggacc cggtccgtta tctcattcaa acatgctggt tactgatcgg
    62161 tacatcgcgc aaagaaacgc cgaaaccgcg caggcgggca ttttcaggtc tgcagaatat
    62221 tcgtggacgt taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca
    62281 gcatcttgat gagaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct
    62341 cggtatcatc gtcaccttct cgctgattct ggcgttaatc tgcgttactc aaccgtttaa
    62401 cccgctggcg cagtttatct tcctgatgct gctgtggggt gtagcgctga tcgtacggcg
    62461 gatgccgggg cgcttctcgg cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg
    62521 ttatatctgg tggcgttaca cctctacgct gaactgggac gatccggtca gcctggtgtg
    62581 cgggcttatt ctgctcttcg ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt
    62641 ccaggtagta tggccgctga atcgtcagcc ggtgccattg ccgaaagata tgtcgctgtg
    62701 gccgtcggtg gatatctttg tcccgactta caacgaagat ctcaacgtgg tgaaaaatac
    62761 tatttacgcc tcgctgggta tcgactggcc gaaagacaag ctgaacatct ggatcctcga
    62821 tgacggcggt agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc
    62881 ccgtaccact catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa
    62941 aggcgagttc gtgtcgattt tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat
    63001 gaccatgggc tggttcctga aagagaaaca gctggcgatg atgcagacgc cgcaccactt
    63061 cttctcgccg gacccgtttg aacgcaacct ggggcgtttt cgtaaaacgc cgaacgaagg
    63121 cacgctgttc tatggtctgg tgcaggatgg caacgatatg tgggacgcca ctttcttctg
    63181 cggttcctgt gcagtaattc gtcgcaaacc attggatgaa attggcggta ttgctgtcga
    63241 aacggtaacc gaagatgcgc acacgtctct gcgcctgcac cgtcgtggct acacctctgc
    63301 atatatgcgt attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg
    63361 tcagcgtatt cgctgggcac gcgggatggt gcaaatcttc cgtctcgata acccgcttac
    63421 cggtaaaggg ctgaaattcg cccagcggct gtgctacgtc aacgccatgt tccacttctt
    63481 gtcgggcatt ccacgactga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc
    63541 ctacatcatc tatgcgccag cgttgatgat cgccctgttc gtgctgccgc atatgatcca
    63601 tgccagcctg accaactcca agatccaggg caaatatcgc cactctttct ggagtgaaat
    63661 ctacgaaacg gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc
    63721 gcacaaaggc aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga
    63781 ctgggtgatc tcgcggccct acatcttcct tgtcctgctc aacctggtgg gcgttgcggt
    63841 aggcatctgg cgctacttct atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat
    63901 ggtgtgggta ttctacaacc tgattgttct tggcggcgca gttgcggtat cggtagaaag
    63961 caaacaggta cgccgatcgc accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga
    64021 agatggtcac ctcttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa
    64081 gatcaacggt caggcgcaga ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg
    64141 tcagcaggaa tacgtcttcc cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct
    64201 gaaattaatg ccgctcacca cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg
    64261 tgcggataca tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct
    64321 gctggatatt ctgaagctcg gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc
    64381 ttcggtgaag ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat
    64441 tccgcgccgc ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca
    64501 atgatgataa cgcgatgaaa agaaaactat tctggatttg tgcagtggct atggggatga
    64561 gtgcgttccc ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg
    64621 agccagctgt agccgcccag acggaacaaa atccgcaggt ggggcaagtg atgccgggcg
    64681 tgcagggcgc tgatgcgcca gtcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga
    64741 cctttgcgca aattgcgccg ccgccgggca gcatggtgct acgtggcatt aacccgaacg
    64801 gcagcattga gtttggtatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacctcg
    64861 aatacacgcc atcgccatcg ttactgcctg tccagtcgca gttaaaggtt tatctcaatg
    64921 atgagctgat gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc
    64981 aaatgcccat taacccactg tttattaccg acttcaaccg tgtgcggctg gagtttgtcg
    65041 gccattatca ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc
    65101 ggagcagtgg actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact
    65161 tcccggtgcc attctttgat ccgcgtgata accgcaccaa caccttgccg atggtctttg
    65221 cgggtgcgcc ggatgttggg ctgcaacaag cctctgccat tgtcgcctcg tggtttggtt
    65281 cgcgttctgg ctggcgtggg cagaacttcc cggtgctcta taaccaactg ccggatcgta
    65341 atgccattgt ctttgcaacc aacgacaaac ggccggactt cctgcgcgat catccggcgg
    65401 taaaagcccc ggtgattgag atgattaacc atccgcagaa tccttacgtc aaactgctgg
    65461 tggtgtttgg tcgtgacgac aaagacctgt tgcaggcagc gaaaggtatc gctcagggta
    65521 acattctgtt ccgtggtgaa agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta
    65581 agccgtacga tgcgccgaac tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga
    65641 aaacctatga agaacagtta caatccagcg gtcttgagcc agcagcgatt aacgtttcgc
    65701 taaacctgcc gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt
    65761 accgctacac catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc
    65821 agttcctgca atccttcaac ctgagcagca aacaggaggc gaaccgcctg ctgctgcgga
    65881 ttccggtatt acaaggtttg ctggatggca aaacagatgt ctctattccg gcgctgaaac
    65941 tgggcgcgac caaccagctg cgcttcgact ttgagtatat gaacccgatg ccgggcggtt
    66001 cggtggataa ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgact
    66061 ccaccatcga cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg
    66121 ctaacgcggg cttcccattc agccggatgg cggatctgtc gcaaaccatc accgtgatgc
    66181 cgaaagcgcc taacgaagca cagatggaaa cgttgctgaa tactgttggt tttatcggcg
    66241 cacagacggg cttcccggcg attaatctga cggtgaccga tgatggcagc accattcagg
    66301 gcaaagatgc cgacatcatg atcatcggtg gtatcccgga caaactgaaa gacgataagc
    66361 agatcgacct attggtgcag gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc
    66421 cgttccccgg cattgtgccg gacgagagcg atcgcgcggc agaaacccag tcaacgctga
    66481 cctcttccgg tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca
    66541 gcgtgattgc gctgctggca gatagcccac gcggttatga aatgcttaac gatgcggtga
    66601 acgatagcgg caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta
    66661 tcaacagcct acgtgttggc gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct
    66721 tgtggtatgc gctggcaaac catccgattc tgctggcggt gctggcggct atcagtgtga
    66781 tattgctggc atgggtactg tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc
    66841 cggataacga gtaattgaag atgaatgtgt tgcgtagtgg aatcgtgacg atgctgctgt
    66901 tggctgcctt tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg
    66961 attacatcag tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct
    67021 ccgaagggca aagttacggt atgttctttg ccctggcggc taacgaccgt gtagctttcg
    67081 ataatattct cgactggacg cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc
    67141 ccgcctggct gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg
    67201 cctccgatgg tgatgtctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag
    67261 agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg
    67321 tgacggtgcc tgggctgggt tccatgttgt taccgggcaa agtgggtttt gctgaggata
    67381 acagctggcg ttttaacccc agctacctgc cgccgacgct ggcgcagtat ttcacccgct
    67441 ttggcgcgcc gtggaccacg ctgcgcgaaa ccaatcaacg tttattgctg gaaaccgccc
    67501 cgaaaggttt ttcgccagac tgggtgcgct atgagaaaga caaaggctgg cagctaaaag
    67561 ccgaaaaaac attgatcagc agctacgacg ctatccgcgt ttacatgtgg gtaggcatga
    67621 tgcctgacag cgatccgcaa aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat
    67681 tcactgagaa aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg
    67741 gtaaaggacc ggtcggtttt tctgccgcca tgctgccctt tttacaaaac cgcgatgcgc
    67801 aggccgttca gcgccagcgc gtggccgata actttcccgg cagcgatgcc tattacaact
    67861 atgtgctgac cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag
    67921 gtgagttatt acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct
    67981 ttccctcggt ctggccgtca tgccgatggt cgaggcagca ccaaccgctc agcaacagtt
    68041 gctggagcaa gttcggttag gcgaagcgac ccatcgtgaa gatctggtgc aacagtcgtt
    68101 atatcggctg gaacttattg atccgaataa cccggacgtc gttgccgccc gtttccgttc
    68161 tttgttacgt cagggcgata ttgatggcgc gcaaaaacag ctcgatcggc tgtcgcagtt
    68221 agcgccgagt tcaaatgcgt ataaatcgtc gcggactacg atgctacttt ccacgccgga
    68281 tggtcgtcag gcactgcaac aggcacgatt gcaggcgacg accggtcatg cagaagaagc
    68341 tgtggcgagt tacaacaaac tgttcaacgg tgcgccgccg gaaggtgaca ttgctgtcga
    68401 gtactggagt acggtggcga aaattccggc tcgccgtggc gaagcgatta atcagttaaa
    68461 acgcatcaat gcggatgcac cgggcaatac gggcctgcaa aacaatctgg cgctattgct
    68521 gtttagtagc gatcgccgtg acgaaggttt tgccgtcctg gaacagatgg caaaatcgaa
    68581 cgccgggcgc gaaggggcct ctaaaatctg gtacgggcag attaaagaca tgcccgtcag
    68641 tgatgccagt gtgtcggcgc tgaaaaaata tctctcgatc tttagtgatg gcaatagcgt
    68701 ggcggctgcg caatcgcaac tggcagaaca gcaaaaacag ctggccgatc ctgctttccg
    68761 cgctcgtgcg caaggtttag cggcggtgga ctctggtatg gcgggtaaag ccattcccga
    68821 actacaacag gcggtgcggg cgaacccgaa agacagtgaa gctctggggg cgctgggcca
    68881 ggcgtattct cagaaaggcg atcgcgccaa tgcagtggcg aatctggaaa aagccctcgc
    68941 actggacccg cacagcagca acaacgacaa atggaacagt ctgctgaaag taaaccgcta
    69001 ctggctggcg atccagcagg gcgatgctgc gctgaaagcc aataatcctg accgggcaga
    69061 acgcctgttc cagcaggcgc gtaatgtcga taacaccgac agttatgcag tgctggggct
    69121 gggcgatgtg gcgatggcgc gaaaagatta tcccgccgcc gaacgttatt atcagcagac
    69181 cttgcgtatg gacagcggca acactaacgc cgtgcgcggg ctggcaaata tttaccgcca
    69241 gcaatcgcca gaaaaagctg aagcgtttat cgcctcgctc tctgccagtc agcggcgtag
    69301 cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggcact
    69361 ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctgga
    69421 ccccggcagc gtatggatta cttaccgact ttcgcaggat ctctggcagg ccggacaacg
    69481 cagccaggcc gatacgttaa tgcgcaatct ggcgcagcag aagccgaacg acccggagca
    69541 ggtttacgct tacgggctgt acctctctgg tcatgaccac gacagagcgg cgctggcgca
    69601 tatcaacagc ctgccgcgcg cgcagtggaa cagcaatatt caggagctgg ttaatcgact
    69661 gcaaagcgat caggtgctgg aaaccgctaa tcgcctgcga gaaagcggca aagaagctga
    69721 agcggaagcg atgctgcgtc agcaaccacc ttccacgcgt attgacctca cgctggctga
    69781 ctgggcgcag cagcgacgtg attacaccgc cgcccgcgct gcatatcaga atgtcctgac
    69841 gcgggagcca actaacgccg atgccattct ggggctgacg gaagtggata ttgctgccgg
    69901 tgacacagcg gcggcacgta gccagctggc gaaactgccc gctaccgata acgcctcgct
    69961 gaacacacag cggcgcgtgg cgctggcgca ggcgcagctt ggagataccg cagcggcgca
    70021 gcagacgttt aataagttga tcccgcaggc aaaatctcag ccaccgtcga tggaaagcgc
    70081 gatggtgctg cgcgacggtg cgaagtttga agcgcaggcg ggcgatccaa agcaggcgct
    70141 ggaaacctac aaagacgcca tggtcgcatc cggtgtgact acgacgcgtc cgcaggataa
    70201 cgacacattc acccgactga cccgtaacga cgagaaagat gactggctga aacgcggcgt
    70261 gcgcagcgat gcggcggacc tctatcgcca gcaggatctt aacgtcaccc ttgagcatga
    70321 ttattgggga tcgagcggca ccggtggtta ctccgatctg aaagcgcaca ccactatgtt
    70381 gcaggtggac gcgccgtatt ctgacgggcg gatgttcttt cgcagtgatt tcgtcaatat
    70441 gaacgtcggc agtttctcca ctaatgccga tggtaaatgg gatgataact ggggcacctg
    70501 tacattacag gactgtagcg gcaaccgcag ccagtcggat tccggtgcca gcgtggcggt
    70561 cggctggcga aatgacgtct ggagctggga tatcggcacc acgccgatgg gcttcaacgt
    70621 ggtggatgtg gtcggcggca tcagttacag cgatgatatc gggccgctgg gttacaccgt
    70681 taacgcccac cgtcggccca tctccagttc tttgctggcc tttggtgggc aaaaagactc
    70741 cccgagcaat accgggaaaa aatggggcgg tgtgcgtgcc gacggcgtgg ggctaagcct
    70801 gagctacgat aaaggtgaag caaacggcgt ctgggcatcg cttagtggcg accagttaac
    70861 cggtaaaaat gtcgaagata actggcgcgt gcgctggatg acgggctatt actataaggt
    70921 catcaaccag aacaatcgcc gcgtcacaat cggcctgaac aacatgatct ggcattacga
    70981 caaagacctg agtggctact cactcggtca gggcggttac tacagcccgc aggaatacct
    71041 gtcgtttgcc ataccggtga tgtggcggga gcacacggaa aactggtcgt gggagctggg
    71101 ggcgtctggc tcgtggtcgc attcacgcac caaaaccatg ccgcgttatc cgctgatgaa
    71161 tctgatcccg accgactggc aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag
    71221 tcagggcttc ggctacacgg cgcgggcatt acttgaacga cgtgttactt ccaactggtt
    71281 tgttggcacg gcaattgata tccagcaggc gaaagattac gcacccagcc atttcctgct
    71341 ctacgtacgt tattccgccg ccggatggca gggtgacatg gatttaccgc cgcagccgct
    71401 gataccttac gccgactggt aagttttcag atagcgcctc tcttaatgcc gctgcgatcg
    71461 ggtatactcg ggcggcaatc tgggatttcc ggggggagac aatttgcgcg taagtcgctc
    71521 gttaacaatc aagcagatgg caatggtggc agccgttgtc ctggtgttcg tttttatttt
    71581 ttgcaccgtt ttgctgttcc atctggtcca acagaatcgc tataacacgg ctacgcaact
    71641 ggaaagcatt gctcgctctg tccgcgaacc cttatcttca gctattttga aaggcgatat
    71701 tcccgaagcg gaagctattc ttgccagcat taaaccggca ggcgtggtca gccgtgccga
    71761 tgtagtgctg cctaaccagt tccaggcgct gcgtaaaagt tttattccag agcgcccggt
    71821 gccggtaatg gttactcgcc tgtttgagct accggttcaa atctcgctgg gcgtttactc
    71881 gctcgaacgt ccggcaaacc cgcagccaat tgcctatctg gtactacagg cggattcctt
    71941 ccgtatgtat aagttcgtga tgagcaccct ctcaacgtta gtgaccattt acttactttt
    72001 gtcgcttatc ctgaccgtcg ccatcagctg gtgcattaac cgcctgattt tgcatccgtt
    72061 acgcaatatt gctcgcgaac ttaacgccat cccagcccag gagcttgttg gtcaccaact
    72121 ggcattaccg cgtctgcatc aggacgatga aatcggtatg ttggtgcgca gttacaacct
    72181 caaccagcaa ttgctgcagc gccattatga agaacagaac gaaaatgcga tgcgcttccc
    72241 ggtgtcggat ttgccgaaca aagccttgct gatggagatg ctggagcagg ttgtcgcgcg
    72301 taaacaaacc accgcgctga tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggcgt
    72361 gctgaaagag gcgcaacgag aaattctgct gctgacgctg gtggaaaaac tcaaatcggt
    72421 actgtcgcca cgtatgatcc tcgcgcagat tagcggttat gactttgctg tcattgccaa
    72481 cggtgtacag gaaccgtggc acgcaatcac cttaggtcag caagtgctca ctatcatgag
    72541 cgagcgcctg ccgattgaac gtattcaact ccgtccgcac tgtagcattg gcgtggcgat
    72601 gttctacggc gatctcaccg ccgaacagct ttacagtcgc gctatttctg cggcatttac
    72661 cgctcgccat aaaggcaaga atcagattca gttctttgat ccgcagcaga tggaagccgc
    72721 ccagaagcgg ttgacggaag agagcgatat ccttaatgca ctggaaaatc atcagtttgc
    72781 tatttggtta cagccacagg tcgagatgac cagcggaaaa ctggtcagtg cggaagtgtt
    72841 actgcgtatc cagcaaccgg atggcagttg ggacctgccg gatggcttaa tcgatcgcat
    72901 tgagtgctgt gggctgatgg ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt
    72961 gcttgcagcc tggcaagagc gcggcattat gctgcccttg tcggtaaacc tctctgcgct
    73021 gcaactgatg cacccaaata tggtggcgga tatgctggaa ctgttaaccc gctatcgcat
    73081 tcagccggga acactgattc tggaagtgac agaaagccga cgtattgacg accctcatgc
    73141 tgcggtggca atcctccgtc cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt
    73201 cggcatgggc tacgcagggc tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt
    73261 actgaaaatc gacaaaatgt ttgttgaagg cttgccggaa gatagcagca tgattgctgc
    73321 aattatcatg ctggcgcaga gcctgaactt acaaatgatt gccgaaggcg tggagactga
    73381 agcacaacgc gactggctgg caaaagcggg cgttggtatt gcccagggct tcctttttgc
    73441 tcgcccactc cctattgaaa tcttcgaaga gagttacctg gaagaaaagt agctacccca
    73501 aactgattac aaaactttaa aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc
    73561 tttttgtttc aattatgatc caggtacatt tctgtgatgt tgtctgggtg ttattttaag
    73621 gccgcaggta ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
    73681 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
    73741 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
    73801 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
    73861 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgag
    73921 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
    73981 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat
    74041 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    74101 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    74161 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtcatcga aagtttctcg
    74221 caggtcatct tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgttcggg
    74281 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    74341 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    74401 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    74461 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    74521 ctcggctgcc gtaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt
    74581 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    74641 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    74701 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    74761 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    74821 cgtgcgctga ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    74881 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    74941 acgcacgaat tatcctctta atctcatcac ttatgcccgc actcccttcc ccgagtgcgg
    75001 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    75061 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    75121 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    75181 catgcagggc acaaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    75241 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    75301 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    75361 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    75421 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    75481 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cgtatgacac
    75541 tacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aggcgctctc
    75601 ttacctggca aatgccactg gcaaactgac tatcacacca gaaaccatca accacgcgct
    75661 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgtta
    75721 tcgtctgaaa ggatcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    75781 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    75841 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg
    75901 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    75961 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    76021 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    76081 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagta acagcaaaga
    76141 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    76201 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    76261 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    76321 ggagctgcag aaactgtttg ccgcctatgc gcgggctgat accgatattc tgatgggtca
    76381 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    76441 gcggcaggat ttccttaaca gcctgacggt ggagatgtta aatcaggatc tgcgtcagca
    76501 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    76561 gaaagcgttg caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac
    76621 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    76681 cctgatggtg atgagttagc acaatcatca ggtatttatg gatgagctgg atagaggggt
    76741 aacactttat ccagcctttt gcatatgctg cttttacgct ggcatcgcct cacgcgggat
    76801 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    76861 ttccgcactg ccgcccgtta aacgtaccgc cagataaccg gcactgaaag agtcgccagc
    76921 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat ccactaaccc
    76981 ttcgccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    77041 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    77101 ttcgtcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgtt ggtacacctg
    77161 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    77221 tccgccgttg gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    77281 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    77341 aatcgccgca gactgctcgc tctccagcca gaatttggca gcggcttcgt tacgccagta
    77401 gtagaacgta cgttcaccgg tgctgtcggt ttcaatgtag taaaggcccg gcagacggtt
    77461 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatttg
    77521 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    77581 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    77641 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    77701 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    77761 gtcaaccata ttaaaacagt gttccattat ttttttgagc caggacgtat tcctgaaaga
    77821 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc
    77881 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    77941 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    78001 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcgga
    78061 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc
    78121 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    78181 gagcagattg atttgctggc gcaaaaagcc gacttcttta tagagcacgg cctgctggca
    78241 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    78301 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    78361 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc
    78421 gggatggcaa atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    78481 gaactgtttg tgatgctgcg tcagtcgccg gaaggacgca cactcttttc tcagctttta
    78541 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac gccggaagag
    78601 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    78661 gcaccgatag aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    78721 tggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    78781 gcaggcaaaa taaccgctgc gatttcagtg gctttcttgt tgttgattgt cgtggcgatc
    78841 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    78901 gcggagttga atcgtccgtt cgctatccgt ggcgatctgg gcgtggtgtg ggagcggcaa
    78961 aaacaagaaa ctggctggcg cagctgggtg ccgtggcccc atgtacacgc ggaagacatc
    79021 attcttggca atccaccgga tattcccgaa gtcacgatgg tgcatttgcc acgcgtagag
    79081 gcaacgctgg ccccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    79141 gaaaagcccg acgcgcgcct gattcgcctc tccgaaaaga acaataactg gacgtttaat
    79201 ctcgccaacg atgataacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    79261 gataatattc ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat
    79321 ctggagattt ttgttgatcc gttaggtaag ccgctgccgt tcagcgaagt tactggatcg
    79381 aaaggtaaag cggataaaga aaaggtgggc gattacgttt ttggcctgaa ggcgcaggga
    79441 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    79501 ggcgaaggga cgccgtttcc ggtacaggct gatttccgct ctggtaacac ccgtgttgct
    79561 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    79621 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    79681 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    79741 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    79801 accaccggaa agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    79861 gcggacctgg gaccgttaat tggcgttgac tccgggaaag gggcagaaaa gtcgaaacgg
    79921 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    79981 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    80041 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    80101 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    80161 atccatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    80221 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    80281 aacggtgacg cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt
    80341 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    80401 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    80461 gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    80521 gctttcgata ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa
    80581 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    80641 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cgggccgttg
    80701 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    80761 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    80821 atgaagaagt gatgaggttg gattgtgttt gctggtgagt tcgggagcag cgtgttcccc
    80881 tcaccctaac cctctcccca aaggggcgag gggactgttt tgtgcgtgtt gaaacaacga
    80941 gtaccgcttt tccgttcact ttctggggga gggctgttat gttcgcgata taacatctcg
    81001 ctccgctttt ctccctctcc ctttcaggga gagggctggg gtgagggtgg ctgcccacac
    81061 gatacttaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat taacgtcaag
    81121 ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagt ctgcaaccag
    81181 gctgcgatat acggcgcaac ggaagccccg agaatcgacg ctacgttgta agagaacgat
    81241 gctccggtgt aacgcacttc tgttgggaac agctctggta acagcgcgcc catcggcccg
    81301 aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag aatcgggttg
    81361 ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag cgtggtgata
    81421 attaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac tggcaccatc
    81481 acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg tggcaggcca
    81541 agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat gatgtaaaac
    81601 agcgtatatg ttgccagcat gatgaacgta cccagtacgg ttacgcgaac atgtttggtc
    81661 agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt ctcaaacacc
    81721 ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac cgccgagaag
    81781 ataaatggca cacgccagcc ccagctcata aactgctcgt cggtcagtag ccaggaaagc
    81841 agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcccagctg tgggaaggag
    81901 ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc cgcgccgccc
    81961 cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag cagcggggcg
    82021 aaaataccaa tcgtggcata gcccggcagc agaccaatca ccacggtcga aatccccatc
    82081 gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa atgaccaaaa
    82141 acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag cgactgtagc
    82201 gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat aacggccgca
    82261 gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga ggcgacaagg
    82321 actttattac gcgagttgat tggcgtgtat tcttgctcgt ggtcgagtgt tgtggctgtt
    82381 gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc acagcaaaag
    82441 tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa aaggggataa
    82501 tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata aatagtggat
    82561 aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg ggttatgagt
    82621 tggaatgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga agagtttcaa
    82681 gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg gcctacgggg
    82741 agccaggccg aataaaatcg tatgccggat aaggcgttta cgccgcatcc ggcctatttt
    82801 aaggctgcgt tttccctggc attcgcgggt cgtctttata ttcggcggtg gcaatccacg
    82861 ccgcgcaaaa cagcgtcaaa cgggcgaaga agtagaaaaa tgccatcagc cccagcacgg
    82921 aaccaaatgc tgcgccagag ggggatttca tcagcgatgg cagggtgtag gtcatcacga
    82981 ttttaatcac ttcaaaacca atagcagcca gaaatgttcc gcgaatcagg gcttttttgc
    83041 gcggacggtg gcgcggtaac cgccagaaga tccagaagaa aagcagatag ttggcgaaga
    83101 tggaaatcgc caggccaatc aaccgccacg tcggtttcag ccactcaatg ctgttcaggt
    83161 gcagggcgct aataatcatt tgctgcgccg aaccggcaac cgaggtgatc gaaagcgtca
    83221 caatcagcgc aatcaacaaa ccaatcagcg aaataaaatc acgcagatat tttacccaga
    83281 acttctcctg atcttgcggc gagcgttccc agacatcgcg cgactgggca cgaatcgctt
    83341 cacgcaggtt acccatccag ttgatgccgg aataaagcgc caccgccagg ccgacaagcc
    83401 ctacagtcgt acgctgctga acggcggtgt tgatggtgtt tttcaacgtg gcggctagcg
    83461 tcggatcgct gatgttttgc agaattttgt cgaagatatc ctgtagcaac atcggatggg
    83521 aggccagcac aaagccccct gcggcaaacg acaccatcaa aatcgggatc atcgataaaa
    83581 atgagaaata ggtgatagcc gcaccaaact ggttacccag ccgatcgtta aagcgttctg
    83641 tcgcgcgaat caggtgcgca ataaccggtt ggcgttggac tttttcggca gtggtggtga
    83701 cggtttccag cgcctggcta actttgccgc ctttctcgct attatccaac tgcttaatcg
    83761 gctcgtgctc cagatcctgg gtgggacgtt tgatctcgtt ttcctgcgtc atccgctctt
    83821 ttgtccttct gtcgtggtca ttcttaaaaa gtatagtcgg tcagtccagg tgctctttca
    83881 ttacgccagc cagccactgc ataaacaggt ttacacgccg ggaaagctcc cgacgctgcg
    83941 ggtaaaccag cgaaagggag agcggctcgg cacggtagcc tggcaacact tcaataaggc
    84001 gcccggcacg cagggcttcg cgcacggcaa tgcgcgggat ctgaataatc cccagaccgg
    84061 caagaccggc ggcgagatag ttttcgctac tatttaccgt caacatgccg ccagacttaa
    84121 accactggac gccattaacg ctggcaacct caaaacctaa cggatgtaca cccaggtgcg
    84181 gtgtataacg cactattgcg tgtgaagtca gatcatcggg gctttgcgga taaccaaagc
    84241 gcgtcagata gtgcggactg gcacagttga ccatggtcag tttgccgagg ggacgggcga
    84301 taacgccatc ttccggtaac gcgccagtgc gtatcacgca atcaaaacca tcatgaagaa
    84361 tatctaccgg acggtcatgg ctactcagtt ccagctcaat tcccggatgc agatagagaa
    84421 attccgacag gcgcggcagt aacaggcttt tcgcgattcc tggcgggatg tcgatgcgta
    84481 atttaccgct gatactggtg gcatcctgtt gaaacagacc gtccagttcg ctgagattac
    84541 tcaacacatc ttttgctcgt tgataatagg tcatgccttc tggcgtgagt ttgacccgtc
    84601 gcgtggtgcg ctggagaagc tgggtgccaa gttgatgttc cagtgcctgt atctggcgcg
    84661 aaacacttcc ttttggcaaa gcaaagaaat ccgccgcgcg ggaaaaactt tccagctccg
    84721 cgactttgat gaacaactgc attgcgtgaa ttttatccat ttctttgacc gattgttgtt
    84781 cacagcgaaa cagtaacgcg catatcgcat tgtttattgt tttttctaac atctaatgcc
    84841 gggtttcaca atgttgtcat gacgaatgtg gcgatttctc agcaggaaaa gggcggaata
    84901 gctttctgag aaaggtgaat ccccctaaac agagcgtatt tttcagcgat tccctttaaa
    84961 gtgaatagag gacgtgttgg ctatttgtgc ttatttttgt ctcattgccg tttgttattt
    85021 ttgcttgtgg tcgcggagtt tcatttttaa ttgcatgcgt actaaccacc aatataagga
    85081 aatcctcaaa attgttttaa atagtgccct cgacatgtaa tttcaggcgt ttagctgtaa
    85141 ctatttatat cggtaactat tttctacgcg tcatctctgt agaatggcca ccgccaaatg
    85201 gcgaattctt attttttctc gcgggtaata cccttccact acttaagtct ttttaacgta
    85261 ttcaggtgga gattcagtta tgcaaatagt catgtttgac aggcagtcaa tatttattca
    85321 tggaatgaaa atcagtttac agcagcgtat tccaggagtg agtattcagg gggccagtca
    85381 ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta tgctcgatgg
    85441 cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc agtttcctga
    85501 ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg aagttattca
    85561 ttttaatgtg ctggccattg tgcctcgtga ttcaaccgtc gagacatttg cgttggcggt
    85621 aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga cgccggaaaa
    85681 ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa ccatgttagc
    85741 ggcgggcgaa tcaaataagg agatcagcag agcgctgaat atcagtaccg gaacggtaaa
    85801 agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc aggcggcaat
    85861 gatgttaaat atctcctcct gaagaggata attttacagc cagcaggaag ttagcggctg
    85921 gctgtaaaaa tattatggtt cgccgtacaa accaattaaa cggcgtacca caccgttagt
    85981 ccagccaaac ccatcctgca acggatactc gccaccgccg ccttcgcggg gaacaccatc
    86041 ggcaatatgg tatttctcga tcattttgtg ctgttccaga tagaactgat tcaccgtttt
    86101 cagccagctg cgcgcgattt catcacccag aaggtcatcg ccatacattt taaatccctg
    86161 aattgccatc cattgtaacg gtgcccagcc attgggttta tcccactgtt caccggtttc
    86221 gtactcgctt gccagaatcc cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag
    86281 acgatcggcc tgttcatggt tcgccatgcc gacatagagc ggcacaatgg cggcagcgga
    86341 aaacagcgcc agttgttcgc gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc
    86401 ccagaggtaa cggtttaccg catcgcgacg ggcactggcc ttctggcgga acagcgcttc
    86461 tgtctctttc tcgcctttca gcgccgagat gttggcgatg gcgctctcca gtttaaacag
    86521 gaaggcattc agatcgatgg ggatgaactg ggtggtacga atgctcgcca gacgaccagt
    86581 atcacgcagc caacgggaag agtaatccca acctgaggcc gctcccgcgc gtaaatcgcg
    86641 gtacacctcg ttgggcgggc gaccagaatg tttcgcggtt tcaacgtcct caagccagga
    86701 ttcgtcacgc ggcgtgtcgc gatcgtccca ataacggttg agcagcgatc cgtccggcat
    86761 ccgcacaaca tggcgatagg cctgattagg gatcaacgat tctgcaccgt ccatccagaa
    86821 ggcatattcc attttaaggt ggtcgagata gcggcgcgca ccgcgtacac catcttcttc
    86881 aaacaactcc accatcagcg caaaaaccgg tggttgcgat cggctcaaat aataggtgcg
    86941 gttgccgttg gggatgtgac catagttttc gatcatccag gcgaagttat cggccatgca
    87001 tttcagcaaa tcttcccgac cactttccgc cagccccagc atggtgaaat aggaatccca
    87061 atagtaggtt tcgctaaaac ggccgcccgg gacaatatat gactgcggta gcgccagcag
    87121 agaagaccac ggaatgtgat cctgtggttc gcgggttagc accggccaca gctggtcgat
    87181 atgctctttc agggaatttt gcgggtccga tacatactcg ctggagtaga cctccggcag
    87241 ccagaagtga ttttcaacaa acttgcgcaa gtcaaaatca cgatgacggc gcactttacg
    87301 gtagcggatt aaaatatcca gcgggtccat tttgggtgcg cagtcgggaa aggttttact
    87361 gtcggggaaa attttcgccg actgaacatg ctcgaacagt tcgagatagc gatcggcagg
    87421 cgtcagcgca tcagaggcgg gcagcccttc aatcatctcg ggttccggtt ctgcctcgat
    87481 catctcatcc agttttaatt catacgggtc cagctcatag cagagatcga cttcgatcat
    87541 cagttcgtct ggattagggt tttgaatttt ctgattgagc ataaggatcc aacctccgaa
    87601 attcgtcgct aattatcggg cttttcttta agggtagtca ttcgctccgc ctggtgggcg
    87661 acaaagcgtg cggtggatca cgtttattaa cctgtcaaaa aatataacgc actaatatcc
    87721 atggaattta taaaaatacg gatttcgtgt tgtttttcat tttcagaaca ttcaatcctg
    87781 ctctcgccgc aatcatgata tgccattcct gatctgggta ataggcacag gctatcttat
    87841 tgatagttta tattcatgta attgattgat tcctatcaaa aagcgtatct ccctgttttt
    87901 tatgatggct ttgtctcgca gagacgtttg cagcattctg gaaaattttc tgggtcacaa
    87961 taactcacca ccgtgaggga agaataatga aaatggtctc acgtattacc gcgatcggcc
    88021 tggctggcgt cgcgatttgc tatttagggt tatctggtta tgtgtggtac cacgataata
    88081 aacgcagtaa acaggccgat gttcaggcat ctgctgtcag tgaaaataat aaggttttag
    88141 gctttctccg cgaaaaagga tgcgactatt gccacacacc ttcggcagaa ttacccgcct
    88201 attattatat tcctggcgcg aaacagttga tggattacga cattaagctt ggatataaat
    88261 cttttaacct cgaggccgtg cgtgcggcac tgctggctga taaacccgtt tcgcaaagcg
    88321 atctgaataa gattgaatgg gtgatgcagt atgaaactat gccaccaacg cgttataccg
    88381 cgctacactg ggcgggtaag gtgagtgatg aagagcgggc ggaaatactg gcctggattg
    88441 caaaacagcg cgcggaatat tacgccagca atgatactgc tccggagcat cgcaatgaac
    88501 cggtgcagcc catcccgcaa aaactgccta ccgatgcgca aaaagtggcg ttgggctttg
    88561 cgctgtatca cgatccccgt ttatcggctg atagcaccat ttcatgcgct cattgccatg
    88621 cgttgaatgc ggggggcgtc gatggcagaa aaacatcgat tggtgttggt ggcgcagttg
    88681 ggccgattaa cgcgccgacg gtatttaact cagtatttaa cgttgagcag ttctgggatg
    88741 gtcgtgcggc aacattgcag gatcaggcag gtggaccgcc gttgaacccg attgaaatgg
    88801 cgtcgaaatc ctgggacgaa attattgcta agctggaaaa agatccgcag cttaaagcgc
    88861 agttcctcga agtttatccg caaggtttca gtggcgaaaa tattactgat gccattgctg
    88921 aatttgagaa aacattaatt acgccggatt ccccatttga taaatggttg cgtggagatg
    88981 aaaatgcgct gacggcgcaa cagaaaaaag gctatcaatt atttaaagat aataaatgtg
    89041 caacttgtca tggtggtatt attctcggcg gacgttcctt tgaaccgttg gggctgaaaa
    89101 aagactttaa ctttggggaa attacggcgg cggatattgg tcgtatgaat gtgactaaag
    89161 aagagcgtga taaattgcgt cagaaagtac ccggtttacg taacgttgct ttaacggcac
    89221 cgtacttcca tcgcggtgac gtgccgacgc tggacggggc ggtgaaactg atgctgcgct
    89281 atcaggtagg caaagagctg ccgcaggagg atgtggatga tatcgtagct ttcctgcaca
    89341 gtctgaacgg ggtgtacacg ccgtatatgc aggataaaca ataattaatt tgatcgcccg
    89401 aacaggaatg tttgggcgat ttttattacg ataataaagt ctgtttttaa tattatcatg
    89461 ttaaatgttt atattataaa aagtcgtttt tctgcttagg attttgttat ttaaattaag
    89521 cctgtaatgc cttgcttcca ttgcggataa atcctacttt tttattgcct tcaaataaat
    89581 ttaaggagtt cgaaatggac cagaagctgt taacggattt ccgctcagaa ctactcgatt
    89641 cacgttttgg cgcaaaggcc atttctacta tcgcggagtc aaaacgattt ccgctgcacg
    89701 aaatgcgcga tgatgtcgca ttccagatta tcaatgatga attatatctt gatggcaacg
    89761 ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc cataaattga
    89821 tggatttgtc gatcaataaa aactggatcg acaaagaaga atatccgcaa tccgcagcca
    89881 tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg
    89941 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
    90001 cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa
    90061 acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac tgggatgtgg
    90121 agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
    90181 aagcctgtga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta
    90241 actatgagtt cccacaaccg ctgcacgatg cgctggataa attccaggcc gacaccggta
    90301 tcgacatcga catgcacatc gacgctgcca gcggtggctt cctggcaccg ttcgtcgccc
    90361 cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata
    90421 aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc
    90481 cgcaggaact ggtgttcaac gttgactacc tgggtggtca aattggtact tttgccatca
    90541 acttctcccg cccggcgggt caggtaattg cacagtacta tgaattcctg cgcctcggtc
    90601 gtgaaggcta
//
LOCUS       DAFAVF010000002        84480 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFAVF010000002 DAFAVF010000000
VERSION     DAFAVF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.002"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            1..2294
                     /gene="yejO"
                     /locus_tag="LGK42_000077"
                     /pseudo
     CDS             1..2294
                     /gene="yejO"
                     /locus_tag="LGK42_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(2398..2474)
                     /locus_tag="LGK42_000078"
     tRNA            complement(2398..2474)
                     /locus_tag="LGK42_000078"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2438..2440),aa:Pro,seq:ggg)
     gene            complement(2549..4309)
                     /gene="pbgA"
                     /locus_tag="LGK42_000079"
     CDS             complement(2549..4309)
                     /gene="pbgA"
                     /locus_tag="LGK42_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBK2656369.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMALLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(4329..4556)
                     /locus_tag="LGK42_000080"
     CDS             complement(4329..4556)
                     /locus_tag="LGK42_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBK2656370.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            4738..5745
                     /gene="yejK"
                     /locus_tag="LGK42_000081"
     CDS             4738..5745
                     /gene="yejK"
                     /locus_tag="LGK42_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBK2656371.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNMSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(5884..6168)
                     /gene="rplY"
                     /locus_tag="LGK42_000082"
     CDS             complement(5884..6168)
                     /gene="rplY"
                     /locus_tag="LGK42_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBK2656372.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(6293..8053)
                     /locus_tag="LGK42_000083"
     CDS             complement(6293..8053)
                     /locus_tag="LGK42_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK2656373.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            8202..8897
                     /gene="rsuA"
                     /locus_tag="LGK42_000084"
     CDS             8202..8897
                     /gene="rsuA"
                     /locus_tag="LGK42_000084"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBK2656374.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRLLTEEEIASVV"
     gene            8925..10115
                     /gene="bcr"
                     /locus_tag="LGK42_000085"
     CDS             8925..10115
                     /gene="bcr"
                     /locus_tag="LGK42_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBK2656375.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            10448..10792
                     /locus_tag="LGK42_000086"
     CDS             10448..10792
                     /locus_tag="LGK42_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656376.1"
                     /translation="MTSLQLSIVHRLPQNYCWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(10796..12385)
                     /gene="yejF"
                     /locus_tag="LGK42_000087"
     CDS             complement(10796..12385)
                     /gene="yejF"
                     /locus_tag="LGK42_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBK2656377.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12387..13412)
                     /locus_tag="LGK42_000088"
     CDS             complement(12387..13412)
                     /locus_tag="LGK42_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2656378.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(13412..14506)
                     /locus_tag="LGK42_000089"
     CDS             complement(13412..14506)
                     /locus_tag="LGK42_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBK2656379.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(14507..16321)
                     /locus_tag="LGK42_000090"
     CDS             complement(14507..16321)
                     /locus_tag="LGK42_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2656380.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFRKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKASKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(16403..17959)
                     /locus_tag="LGK42_000091"
     CDS             complement(16403..17959)
                     /locus_tag="LGK42_000091"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBK2656381.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(18140..18706)
                     /gene="mepS"
                     /locus_tag="LGK42_000092"
     CDS             complement(18140..18706)
                     /gene="mepS"
                     /locus_tag="LGK42_000092"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBK2656382.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRVVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(19118..19831)
                     /gene="lpxT"
                     /locus_tag="LGK42_000093"
     CDS             complement(19118..19831)
                     /gene="lpxT"
                     /locus_tag="LGK42_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBK2656383.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVYHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(19870..20856)
                     /gene="yeiR"
                     /locus_tag="LGK42_000094"
     CDS             complement(19870..20856)
                     /gene="yeiR"
                     /locus_tag="LGK42_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBK2656384.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(20974..22440)
                     /locus_tag="LGK42_000095"
     CDS             complement(20974..22440)
                     /locus_tag="LGK42_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK2656385.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(22663..23235)
                     /gene="yeiP"
                     /locus_tag="LGK42_000096"
     CDS             complement(22663..23235)
                     /gene="yeiP"
                     /locus_tag="LGK42_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBK2656386.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            23390..23644
                     /locus_tag="LGK42_000097"
     CDS             23390..23644
                     /locus_tag="LGK42_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBK2656387.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(23641..24822)
                     /gene="setB"
                     /locus_tag="LGK42_000098"
     CDS             complement(23641..24822)
                     /gene="setB"
                     /locus_tag="LGK42_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBK2656388.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            25190..26320
                     /gene="fruB"
                     /locus_tag="LGK42_000099"
     CDS             25190..26320
                     /gene="fruB"
                     /locus_tag="LGK42_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBK2656389.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            26320..27258
                     /gene="fruK"
                     /locus_tag="LGK42_000100"
     CDS             26320..27258
                     /gene="fruK"
                     /locus_tag="LGK42_000100"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK2656390.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            27275..28966
                     /gene="fruA"
                     /locus_tag="LGK42_000101"
     CDS             27275..28966
                     /gene="fruA"
                     /locus_tag="LGK42_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBK2656391.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            29390..30331
                     /locus_tag="LGK42_000102"
     CDS             29390..30331
                     /locus_tag="LGK42_000102"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBK2656392.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            30319..31257
                     /gene="psuG"
                     /locus_tag="LGK42_000103"
     CDS             30319..31257
                     /gene="psuG"
                     /locus_tag="LGK42_000103"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBK2656393.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            31351..32601
                     /locus_tag="LGK42_000104"
     CDS             31351..32601
                     /locus_tag="LGK42_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK2656394.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(32672..33370)
                     /gene="yeiL"
                     /locus_tag="LGK42_000105"
     CDS             complement(32672..33370)
                     /gene="yeiL"
                     /locus_tag="LGK42_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBK2656395.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            33500..34441
                     /gene="rihB"
                     /locus_tag="LGK42_000106"
     CDS             33500..34441
                     /gene="rihB"
                     /locus_tag="LGK42_000106"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBK2656396.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            34541..35791
                     /locus_tag="LGK42_000107"
     CDS             34541..35791
                     /locus_tag="LGK42_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK2656397.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(35847..36935)
                     /locus_tag="LGK42_000108"
     CDS             complement(35847..36935)
                     /locus_tag="LGK42_000108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK2656398.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(36939..37796)
                     /gene="nfo"
                     /locus_tag="LGK42_000109"
     CDS             complement(36939..37796)
                     /gene="nfo"
                     /locus_tag="LGK42_000109"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK2656399.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(37870..38919)
                     /locus_tag="LGK42_000110"
     CDS             complement(37870..38919)
                     /locus_tag="LGK42_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBK2656400.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            39018..39899
                     /locus_tag="LGK42_000111"
     CDS             39018..39899
                     /locus_tag="LGK42_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2656401.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            40104..41573
                     /gene="lysP"
                     /locus_tag="LGK42_000112"
     CDS             40104..41573
                     /gene="lysP"
                     /locus_tag="LGK42_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBK2656402.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            41866..43857
                     /gene="cirA"
                     /locus_tag="LGK42_000113"
     CDS             41866..43857
                     /gene="cirA"
                     /locus_tag="LGK42_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBK2656403.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNAVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(43889..44725)
                     /gene="yeiG"
                     /locus_tag="LGK42_000114"
     CDS             complement(43889..44725)
                     /gene="yeiG"
                     /locus_tag="LGK42_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBK2656404.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            44983..45651
                     /gene="folE"
                     /locus_tag="LGK42_000115"
     CDS             44983..45651
                     /gene="folE"
                     /locus_tag="LGK42_000115"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBK2656405.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            45668..46825
                     /locus_tag="LGK42_000116"
     CDS             45668..46825
                     /locus_tag="LGK42_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBK2656406.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            46967..48007
                     /gene="galS"
                     /locus_tag="LGK42_000117"
     CDS             46967..48007
                     /gene="galS"
                     /locus_tag="LGK42_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBK2656407.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            48287..49285
                     /gene="mglB"
                     /locus_tag="LGK42_000118"
     CDS             48287..49285
                     /gene="mglB"
                     /locus_tag="LGK42_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBK2656408.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            49346..50866
                     /gene="mglA"
                     /locus_tag="LGK42_000119"
     CDS             49346..50866
                     /gene="mglA"
                     /locus_tag="LGK42_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBK2656409.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            50882..51892
                     /gene="mglC"
                     /locus_tag="LGK42_000120"
     CDS             50882..51892
                     /gene="mglC"
                     /locus_tag="LGK42_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBK2656410.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(51963..53198)
                     /gene="preA"
                     /locus_tag="LGK42_000121"
     CDS             complement(51963..53198)
                     /gene="preA"
                     /locus_tag="LGK42_000121"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBK2656411.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(53192..54430)
                     /gene="preT"
                     /locus_tag="LGK42_000122"
     CDS             complement(53192..54430)
                     /gene="preT"
                     /locus_tag="LGK42_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBK2656412.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(54749..54988)
                     /locus_tag="LGK42_000123"
     CDS             complement(54749..54988)
                     /locus_tag="LGK42_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBK2656413.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(54991..55710)
                     /gene="sanA"
                     /locus_tag="LGK42_000124"
     CDS             complement(54991..55710)
                     /gene="sanA"
                     /locus_tag="LGK42_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBK2656414.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(55860..56744)
                     /gene="cdd"
                     /locus_tag="LGK42_000125"
     CDS             complement(55860..56744)
                     /gene="cdd"
                     /locus_tag="LGK42_000125"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK2656415.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAENADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(56874..57569)
                     /locus_tag="LGK42_000126"
     CDS             complement(56874..57569)
                     /locus_tag="LGK42_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBK2656416.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(57566..57964)
                     /locus_tag="LGK42_000127"
     CDS             complement(57566..57964)
                     /locus_tag="LGK42_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK2656417.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            58203..59150
                     /gene="dusC"
                     /locus_tag="LGK42_000128"
     CDS             58203..59150
                     /gene="dusC"
                     /locus_tag="LGK42_000128"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBK2656418.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(59523..59606)
                     /gene="yohP"
                     /locus_tag="LGK42_000129"
     CDS             complement(59523..59606)
                     /gene="yohP"
                     /locus_tag="LGK42_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBK2656419.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            59830..61266
                     /gene="mdtQ"
                     /locus_tag="LGK42_000130"
     CDS             59830..61266
                     /gene="mdtQ"
                     /locus_tag="LGK42_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBK2656420.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            61319..62080
                     /locus_tag="LGK42_000131"
     CDS             61319..62080
                     /locus_tag="LGK42_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2656421.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(62210..62788)
                     /locus_tag="LGK42_000132"
     CDS             complement(62210..62788)
                     /locus_tag="LGK42_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2656422.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            62958..63545
                     /locus_tag="LGK42_000133"
     CDS             62958..63545
                     /locus_tag="LGK42_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBK2656423.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            63719..64648
                     /gene="pbpG"
                     /locus_tag="LGK42_000134"
     CDS             63719..64648
                     /gene="pbpG"
                     /locus_tag="LGK42_000134"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBK2656424.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAGQTAQND"
     gene            complement(64686..66401)
                     /gene="dld"
                     /locus_tag="LGK42_000135"
     CDS             complement(64686..66401)
                     /gene="dld"
                     /locus_tag="LGK42_000135"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBK2656425.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            66597..68894
                     /gene="bglX"
                     /locus_tag="LGK42_000136"
     CDS             66597..68894
                     /gene="bglX"
                     /locus_tag="LGK42_000136"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBK2656426.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            69105..70022
                     /gene="osmF"
                     /locus_tag="LGK42_000137"
     CDS             69105..70022
                     /gene="osmF"
                     /locus_tag="LGK42_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBK2656427.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            70029..71186
                     /gene="yehY"
                     /locus_tag="LGK42_000138"
     CDS             70029..71186
                     /gene="yehY"
                     /locus_tag="LGK42_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBK2656428.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGFGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            71179..72105
                     /gene="yehX"
                     /locus_tag="LGK42_000139"
     CDS             71179..72105
                     /gene="yehX"
                     /locus_tag="LGK42_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBK2656429.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            72110..72841
                     /gene="yehW"
                     /locus_tag="LGK42_000140"
     CDS             72110..72841
                     /gene="yehW"
                     /locus_tag="LGK42_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBK2656430.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(72822..72929)
                     /locus_tag="LGK42_000141"
     CDS             complement(72822..72929)
                     /locus_tag="LGK42_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBK2656431.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(72989..73720)
                     /gene="mlrA"
                     /locus_tag="LGK42_000142"
     CDS             complement(72989..73720)
                     /gene="mlrA"
                     /locus_tag="LGK42_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBK2656432.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            73942..75627
                     /gene="btsS"
                     /locus_tag="LGK42_000143"
     CDS             73942..75627
                     /gene="btsS"
                     /locus_tag="LGK42_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBK2656433.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            75624..76343
                     /gene="yehT"
                     /locus_tag="LGK42_000144"
     CDS             75624..76343
                     /gene="yehT"
                     /locus_tag="LGK42_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBK2656434.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            76390..76860
                     /locus_tag="LGK42_000145"
     CDS             76390..76860
                     /locus_tag="LGK42_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK2656435.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(76901..77362)
                     /locus_tag="LGK42_000146"
     CDS             complement(76901..77362)
                     /locus_tag="LGK42_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBK2656436.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(77487..79487)
                     /locus_tag="LGK42_000147"
     CDS             complement(77487..79487)
                     /locus_tag="LGK42_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBK2656437.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(79484..80620)
                     /locus_tag="LGK42_000148"
     CDS             complement(79484..80620)
                     /locus_tag="LGK42_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK2656438.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(80613..82892)
                     /locus_tag="LGK42_000149"
     CDS             complement(80613..82892)
                     /locus_tag="LGK42_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656439.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(82903..83991)
                     /locus_tag="LGK42_000150"
     CDS             complement(82903..83991)
                     /locus_tag="LGK42_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK2656440.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        20614 a        20906 c        22377 g        20583 t
ORIGIN      
        1 atcaaattgt tcaaatgggt ggcgcggcaa accagactac cctcaataat ggtgtgctac
       61 aggtttatgg cgcagcgaat gatcccacga ttaaaggcgg gcgcttaatc gttgaaaaag
      121 atgggattac cgtccttgcc gctatcgaaa agggaggatt actggaggtt aaagaggggg
      181 gattagcgat tgcggtagat cagaaagcag gcggtgctat taaagcaagc acgcgggtca
      241 tggaggcatt cggaacaaac cgtctcggtc agttcgaaat caagaatggt attgctaaca
      301 atatgctgtt ggaaaacggc ggaagtttgc gagttgaaga aaatgacttc gcttataata
      361 ctactgtaga tagtggcggc ttactggagg ttatggatgg cgggactgta actggcgttg
      421 ataaaaaagc aggcggaaaa ttaattgtct caacgaatgc gctggaagtg agtggtacaa
      481 acagtaaagg ccaatttagt ataaaagatg gtgtgtcaaa aaattatgaa ctggatgatg
      541 gttccgggct cattgttatg gaggacacgc aggccattga tactatcctt gatgagcatg
      601 ccactatgca atcgctggga aaggatactg gtacgaaagt gcaggcaaat gcggtatatg
      661 atctcggtcg atcagatcag aatggaagta tcacgtattc ctcaaaagcc atttctgaaa
      721 atatggttat caacaatggc cgcgctaacg tctgggctgg cacaatggtt aacgtttcag
      781 tcagagggaa tgatggcatt cttgaggtca tgaagccgca aataaattat gcacccgcaa
      841 tgttggtggg taaggtagtg gtttctgagg gcgcttcttt tagaacgcat ggtgccgtgg
      901 ataccagcaa agcggacgtt tcgctcgaaa atagcgtatg gaccatcatt gccgatatca
      961 ctacgacgaa ccaaaacacc ctcctcaact tagccaacct tgcgatgtct gacgcaaatg
     1021 tgattatgat ggatgagcca gtgactcgtt catcagtgac ggcaagtgcg gaaaatttta
     1081 ttacgttgac caccaatacc ctgtcgggaa acggcaattt ttatatgcgt accgatatgg
     1141 ctaatcatca gagcgatcag ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat
     1201 tcgtgacgga caccggtgcc agcccggcag caggagatag ccttacactg gtaacaacgg
     1261 gcggcggtga tgctgcattt acgttgggca atgccggagg cgttgttgat atcggtacgt
     1321 atgaatatac cttgctggat aatggcaacc atagctggag tttggcagag aatcgcgcgc
     1381 aaattacccc ttcaaccact gatgtgctga atatggcggc agcacaaccg ctggtatttg
     1441 atgcagaact ggacaccgtg cgtgagcgtc ttggtagcgt aaaaggcgtt aattacgata
     1501 cggcgatgtg gagttcggca attaacaccc gcaacaacgt gaccactgat gcgggcgctg
     1561 gctttgagca aacattgacc gggctgacgc tcggtatcga tagccgtttc tcccgtgaag
     1621 aaaccagcac aattcgcggc ttgttctttg gttactctca ttctgatatt ggttttgatc
     1681 gcggcggcaa aggcaatgtc gatagctata ccctgggggc ttatgccggt tgggagcatc
     1741 agaacggtgc ctatgttgat ggagtggtga aagttgaccg ttttgccaac accatccatt
     1801 gcaagatgag taatggggca acagcgtttg gcgattacaa tagtaacggc gcgggtgctc
     1861 atgtcgagag cgggttccgt tgggttgacg gattgtggag tgttagaccc tatctggcct
     1921 ttaccggctt taccacagat ggtcaggact acacgttatc aaacggcatg cgcgcggatg
     1981 tgggaaatac ccggatatta cgcgctgaag cgggaacggc ggtaagctat cacatggacc
     2041 tgcaaaacgg tacgacgctg gaaccctgac tgaaagccgc cgtgcgtcag gaatacgccg
     2101 attctaacca ggtgaaagtt aatgacgatg gcaaatttaa taatgatgtg gctggaaccc
     2161 gtggcgttta tcaggctggg ataaggtcat cgtttacccc gacgttaagc ggtcatttgt
     2221 cagtcagcta tggcaatggc gcaggggtag aatcgccgtg gaatactcag gcgggtgtgg
     2281 tctggacgtt ctgataacaa aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga
     2341 attaattgca gatataaaaa aaaccaaccg taaaggttga ttttttcttg ggatttttgg
     2401 tcggcacgag aggatttgaa cctccgaccc ccgacacccc atgacggtgc gctaccaggc
     2461 tgcgctacgt gccgactcgt ggctgctaat actaccgttt tccacaccga ttgcaagtaa
     2521 gatattttgc taactgattt ataattaatc agttagcgat aaaacgcttc tcgtctgtca
     2581 gcacttgcaa taacaaactt aactgtggtt tttcatcttt cactctttca ccacgtaagt
     2641 tgtaagtgcg gtatttaccg ttattgttca gcaccagcgt ctttttcggg gtggtaattg
     2701 ccagcgtatc gttatccgct gcagtaaccc agtaatgacg gcgttgaggg ttgaacaaat
     2761 cctgaccttg cgaatattcg ctggcaggtg tgctgacatg tagcaggcgt tgcatcagcg
     2821 tcgtcatcag atcggtatga tcagtcagag cattaatacg ctgcgccggc gtgcctggcc
     2881 agtgaatcac taatggcacc tgcagatgac cgtgggacca gtcaaaggtt tcttcgtctt
     2941 cgctcagcgg aataccccga ccggcagtga taatcaccac cgtattgtcc agtttgccag
     3001 aatcacgcag tgcattgagc acgcggttga tctggtcatc gacattgcct gccgcccggc
     3061 tatatttccg tgcaaatgcc tgctgattgc tgtcgtcaat gttagtgcca ttgaaagaga
     3121 cccacgagaa ccagcggtta tcttcttgtg cgtagcggcc cagccagttg atccactgcg
     3181 tggcggtctg ctcgtcggat tgggtgcgta cgctcggcat cgagaaatct gacaacaatg
     3241 cctggcgata cagcgggctg gtaaagccat ctgatgagaa taatcccagc tgatagcctt
     3301 gctgattaag cgcagtaatt aacgccgcag gggtacgggt cgacagaatg ccgtccatat
     3361 agctcggcga gatgccatag aacaggccaa agatgccgtt gtctgtagtg ttgccggagc
     3421 tcatatggcg cgtgaacgaa atattttgct cagcaaaacc tgccagcgca ggcatctgct
     3481 tctcgaagcg tgagtagttc aggccatcga cagtaatcaa cagcacattc tgcccggtgc
     3541 ccatatcgcg atagcgcagt tcgcttaacg gatactgaac ggaaacggcg tctggattac
     3601 cttgctcaat aagacggcgt tgatactcct gcgcatcaag cagaccatgc ttctcaagaa
     3661 aacgtcgcgc cgtcatcggg tacgaaagcg gcaggttagc gcgctgcatg gtgatcgggc
     3721 gatagaagtt ggcatcggcc cagatataca ccacatgcga ggcgataaag gcgataaata
     3781 agaatgcggc cagcgggcgc gcgaagcgcc gacgacgcgt caggctgcgc agcttttgcc
     3841 agctccacgt cgcaaacacc agttcaagca ataaaataac cggcacgctg atgaacatca
     3901 gctgccagtc gcgcgccatc tcattttcgt ctgggttgat aaccagttgc cagacgatgg
     3961 gattaagatg gagatggaaa cgagtaaaga cttcgctatc gatcagtaat agcgccattc
     4021 ccgccgttgc cagaatgacg gacaaaaacc tcatcagcct ctgggagccg acgataaagg
     4081 tcagcgggaa gaggatcagc aagtaggtgg cgaacaccag gaagctgaaa tggccgataa
     4141 tgcttacgta ggaataaatg cgaccagcaa gcgttgtcgg ccagtcggcg ataaacaggt
     4201 aacggctgcc aatgacgagc gaaagcagaa tattgaacag tgcaaaccag tgcccccaac
     4261 tgaccatctg ggagactttt tcacggtagc gctgacgatg agttaccata acctgttatc
     4321 tgtttccctt agtgcgcttt gtcttcgttg atagaggact gtaaggcgcg ggcaaaagag
     4381 ttggcaattg cctggcgttg ggccggggca atgctggtat tgataaggtt agtgaccatg
     4441 ttgcctaaca ccatcaggga aaggtcggtc ggagccttat gtttttccag tacgttgagc
     4501 agctctgcga gcaattgttc aacctgttca tcactgtagc gggaaatttg tggcataaat
     4561 cgaaatcagt tagttgatga aagggcaaca tattaccgta gcaacggttt tttttctgct
     4621 tttttatgcg ttaaatcatt ctcgttacgc tgccttctgt aaaatgaccg ccatctttta
     4681 ttgcgtcgct taaccggcgg tggttgaata ccgcccggtc ttaaaggaga gtttatcatg
     4741 agtctggata tcaaccagat tgctctgcac cagcttatca agcgtgatga gcagaacctt
     4801 gagctggtgt tgcgcgattc attgctggaa ccgacagaaa ccgtcgttga gatggtggca
     4861 gaactgcatc gggtctatag cgccaaaaat aaagcttacg gcttgtttag cgaagagagt
     4921 gaactggcgc agacgctgcg cttacagcgt cagggcgaag aggactttct ggcatttagc
     4981 cgcgcggcaa ccgggcgcct gcgtgacgaa ttagcgaaat atccttttgc tgacggtgga
     5041 ttcgtgctgt tttgccacta ccgttatctg gcggtggagt atctgctggt ggcagttctg
     5101 agcaacatga gcagtatgcg cgttaacgaa aatctcgaca tcaatccaac ccattatctt
     5161 gatatcaatc atgcggatat tgttgcgcgt atcgatttaa ccgaatggga aaccaatcca
     5221 gagtccaccc gctatctcac tttcctgaaa gggcgagtag ggcgcaaagt ggcggatttc
     5281 tttatggatt tcctcggggc cagcgaaggg ttaaacgcga aagcgcaaaa ccgtgggctg
     5341 ttgcaagcgg ttgatgattt cactgccgaa gcacaactgg ataaagcaga gcgacagaac
     5401 gtgcgccagc aggtttatag ctactgtaat gagcagctgc aggcaggcga agagatcgaa
     5461 ctggagtcgc tgtcgaaaga gctggcaggc gtcagcgaag tgagtttcac ggagtttgcg
     5521 gctgaaaaag gctacgaact ggaagagagc ttcccggcag accgtagcac actgcgtcag
     5581 ttgacgaagt ttgccggtag cggcggtggc ttgactatta actttgatgc gatgctgtta
     5641 ggtgaacgca tcttctggga tccggcaacc gataccctga ccattaaagg cacgccgccg
     5701 aatttgcgtg accaattgca gcgccggacg tctggcggca attaatcggt tttgtgttct
     5761 gaatgccaga tgcggcgtga acgccttatc tggcctgcaa aaaatagcaa taccgtaatc
     5821 aggcataatc tgcagataca aaaaaccccg ccggagcggg gttttttaca acttattcag
     5881 caattaagcg cgaacgaagt cgatgtgctg cagcttcggt ttgtacgggt gacgctgtac
     5941 gtcctgagct ttaactttga tttctttacc gtcaacaacg atggtcagaa cttcgctgta
     6001 gaattcagct ttagcttgca tgttcatgac tttgtcgtga tccagctcga tagccagcgg
     6061 cgcttctttg ccaccgtaga tgattgccgg gaacttgtta gcggcacgca ggcggcggct
     6121 cgcaccctta ccctgctctt tacgtacttc tgcgttgata gtaaacattt ctttctctct
     6181 taaattaatc ctgctacagg cgacccagca acaggtaagt gatctgcttt gcgtatgcaa
     6241 aagcgggcga gattctatac tcaatcgctt tatacatcaa tcaaaactac cattaaccgc
     6301 gtaattcgtg cgcccgacga aaacgacctt cgtaatcgaa cactttttca cgcacttgcc
     6361 agtactgacc tttcatgcgg gcaacgacaa aatccgggtg tcgcaataag gcttgctggg
     6421 cgaggatatc ggcggcggtg atccagcgca gagggatgcc cggtgtgcgc gtatgcgggc
     6481 ggataaaaag ctgctcgaag gcagttcgct gggcgggcgt ttgcagacgg aaacgctcac
     6541 tcacatccgc gccgtcttca tcgtaatagg tgattttcaa ccattcgcct ttctcgtcgt
     6601 gctcatgctg caaagacatg ccgctacagc gtaataccag cgcatctttc agtcgtagcg
     6661 ccgcttttaa catatcatcc ggatcaacca gtaccgtgtc acattcgcgg cagcggcgag
     6721 cggcaatatc attttctgcg ttacattgcg ggcaattttt aaaacggaaa cggaagtcac
     6781 actgttcgcg atgaccgtcg tcatcttcaa accatccctg acagcggcga ccaaagtgtt
     6841 caatcaatgt cccgtcggcg gtcgttttcc cccaaaaggt gttggcaaaa ccgcaggcag
     6901 ggcagaaaac ctgtaccgga acgttgtcac ttttgccttt cggtgtacca acttccggcg
     6961 cgtagagatc gtgaggatta cccgcataat caagaattaa acaatcagtc ttgcccggag
     7021 cgagacgcag accgcgcccg acaatttgtt ggtaaagact cactgattcg gtagggcgca
     7081 gaatggcgat aagatcgacg tgcggggcgt caaatccggt ggtcagtact gcgacgttga
     7141 ccagatagcg aaaacgctgg gctttaaaat cttcaattaa cacatcgcgc tcagcgccgg
     7201 gggtgtcgcc agtaatcagt gcagcatctt cggcaggcag taatcccaca atctcttttg
     7261 cgtgttcaac ggtggcggcg aaaatcatta cccctttgcg cttttcggcg aactccatga
     7321 tctggctgat gatatgtggc gtaatgcgtt gctgtttttt cagctcacgg ttgagatcag
     7381 cttcgctgaa cagcccgtta ctctgtgcct gcaagcggct gaaatcgtat tgcactactg
     7441 gcatatccag tcgttctggc ggcgtcagat agccgtgttt aatcatataa cgcagcggca
     7501 gctcataaat gcagtcacgg aaaagggctt tctcatcgcc gcgtaccatg ccgtgataat
     7561 ggaactggta gatccagcct ttgcccaatc gaaaaggcgt ggcagtcagc cccagcaggc
     7621 gtaagtgggg attcacttta gtcaggtgag tgaggatttg ctgatactgg ctctcttcat
     7681 cgtcaccaat acggtggcat tcatcgacaa tcaacagcga aaattcaccc tgaaaggcat
     7741 caagattacg agcgaccgac tgcacgctgc caaataccac tttaccgtgg ctctctttgc
     7801 gctttaaccc agcggcaaaa atatcggctt ccagccccag cgcctgatac tttgcatggt
     7861 tttgcgccac cagttctttg acgtgtgcca gcaccagaac acgaccacgc gccagccgtg
     7921 ccagttcggc aatcaccagg cttttacctg cgccggtggg cagcacgata acggcagggg
     7981 ttttatgacg acgaaaatgg ttgagcgtgg catccacggc ttcttgctga tatgggcgaa
     8041 gtgtaaaaat catggtctcg cgtaggtaga ttattctgct aatagtatgc catggatctt
     8101 tttcccttga gggatgttat tagaccgcta tactgggcag acaacagttc agattttcgg
     8161 gtcaatgccc gacctccgtt tttacaggca aaatcacaca catgcgactt gataaattta
     8221 tcgcacagca actcggcgtt agccgtgcta ttgccgggcg tgaaatccgc ggcaatcgtg
     8281 tcaccgtcga tggcgaaatc gtccgtaatg cagcgttcaa actgcttcct gaacatgatg
     8341 tcgcttacga tggcaacccg ctggcgcagc aacacggtcc acgttacttc atgctcaata
     8401 agcctcaggg ctatgtttgc tccacggacg accctgatca cccaacggtg ctctattttc
     8461 ttgatgaacc ggtagcgtgg aaactgcatg cggcggggcg gttggatatt gataccaccg
     8521 gtctggtgct gatgactgat gatggtcagt ggtcgcaccg cattacttct ccgcgccatc
     8581 attgcgagaa gacctatctg gtgacactgg aatcacctgt agctgacgat acggcagagc
     8641 aatttgctaa aggcgtgcag ctgcataacg aaaaagatct cactaagcct gcggtgctgg
     8701 aagtgattac cccaacgcag gttcgtctga ccatcagcga agggcgttat catcaggtga
     8761 aacgcatgtt cgccgccgtg ggtaaccacg tggttgagct gcatcgtgaa cgtattggcg
     8821 gtattacgct ggatgctgat ttagcccccg gtgaatatcg tctgttaact gaagaagaaa
     8881 ttgccagcgt cgtctaactt ttcaatgact ttcaggagcc cgttgtgacc acccgacagc
     8941 attcgtcgtt tgctattgtt tttatccttg gcctgctggc catgttgatg ccgctgtcga
     9001 ttgatatgta tctgcccgcg ctaccggtaa tttcagcgca gtttggcgta ccggcgggca
     9061 gtacgcagat gaccctcagt acttatattc tgggctttgc gttggggcag ttaatctacg
     9121 ggccgatggc agacagcttc gggcgtaagc cggtggtgct cggcggtacg ctggtgtttg
     9181 ccgccgccgc ggtggcgtgt gcgttggcaa acaccatcga tcagctgatt gtgatgcgtt
     9241 tcttccacgg gctggctgcg gctgcggcca gcgtggtcat taacgccctg atgcgcgata
     9301 tttacccgaa agaagagttc tcgcggatga tgtcgtttgt catgctggtg accactattg
     9361 caccgctgat ggcaccgatt gtcggcggtt gggtgctggt gtggctgagc tggcattaca
     9421 tcttctggat cctggcatta gcggcgattc tggcttcggc aatgattttc ttcctgatta
     9481 aagaaacctt accaccggag cgtcgtcagc catttcacat tcgtaccact attggtaact
     9541 ttgcggcgct gttccgtcat aagcgtgtcc tgagttatat gctggccagc ggtttcagct
     9601 ttgccggaat gttctcgttc ttaagcgccg gaccgtttgt ctacattgaa attaaccacg
     9661 tcgcgccgga aaactttggt tattactttg cactaaacat tgttttcctg ttcgtgatga
     9721 ccatttttaa cagccgcttc gttcgccgca ttggcgcgtt aaatatgttc cgctcggggt
     9781 tgtggataca atttattatg gcagcgtgga tggtcatcag tgcgctgctg gggctgggat
     9841 tctggtcgct ggtggttggc gttgcggcgt ttgtgggctg cgtgtcgatg gtgtcatcca
     9901 atgcgatggc ggtcattctt gatgagtttc cccatatggc gggaacggca tcttcgctgg
     9961 caggaacctt ccgttttggc ataggggcaa ttgttggcgc attgctttct cttgcgacct
    10021 ttaactctgc atggccgatg atttggtcaa ttgcattctg cgcaagcagc tccattctct
    10081 tctgtctgta cgccagtcga ccgaaaaaac ggtgatctat tgcacaacga ggaagctaaa
    10141 aggcttcctt tgttgatgca tgtcaaccac aaatctatca ttcccccgat atatgtttat
    10201 tttatgtaaa atcaatttat gtaaaaagtc acatcattgt agttaaaaag gttgagttag
    10261 atcgcagaaa cgggtacata tagccccgca aacgtgacca cgcccgcaga tattacttaa
    10321 atcagagcca tagaggccac gcaggcgagg catcaatctt tacgatctgt ataaagacgg
    10381 attgttgatg atgtgttaaa attgatgtaa acaaattgtg aagtgaatgt acttccgggg
    10441 aaaataagtg acttcattac aactctcaat cgtccatcga ctgccgcaga actattgctg
    10501 gtctgctggt ttcgcaggtt cgaaggttga accgattccg caaaatggac cgtgcggtga
    10561 caacagcctg gtggcgctta aattgcttag cccggatggt gataatgcat ggtcggtgat
    10621 gtataaacta agccaggcgt taagcgacat cgaagttcca tgttcggtgc tggagtgtga
    10681 aggtgagccg tgcctgtttg taaatcgcca ggacgagttt gctgcaacat gccgattgaa
    10741 aaattttggt gtggcaattg ccgaaccctt ttcaaactac aatccttttt aagcgtcagc
    10801 tcaacgccag tagctgacgc gtatactcct gctgcggtgt ggcaaacacg cgcgcgcacg
    10861 gtccttgttc cactacttcc ccttgtcgca gtacgataac ctgatgacat aacgcgcgga
    10921 caacgtgcaa atcgtggctg ataaacaaat aggccagttg atgcttttgt tgcaatgatt
    10981 tcaatagcgt caatatttgc gcctgtaccg ttttgtcgag tgatgatgtc ggttcatcaa
    11041 gtatgatcag cgagggctta agaattaatg ccctggcaat cgcaatacgt tgtcgctgac
    11101 caccagagaa ctccgccgga taacggtggc gtgtttcagg atctaatccc acttcatgca
    11161 tcacggctat cacttgttgt tcgcgttgtg cggcagaaag cgtcggctgg tgaacccgta
    11221 agccttcctc aataatctgc aaaacgttga gtcgtgggtt gagcgaggag tttggatcct
    11281 gaaataccac ctgaatgcga tgacgaatag gtaacagctg gcggcgattt aaattttgca
    11341 gtggctgacc gtcaaagatg atgctgccct gagaattaat cagtcgcagc agcgccagtc
    11401 ccgtcgtact tttcccggaa ccggactcgc ccactaaacc cagtgtttca cccgctcgta
    11461 gcgtaaaact gatgtttttc accaccacat tatgatccac aatgcgcttc aaaatccctt
    11521 tgcgaatggg gaaggcaacc tgaagctgtt caacatccag caacgttgag gcaggttctg
    11581 gcaacggcac tggatcgcct gacggttcac tgttgagtag cttttgtgtg taaggatgag
    11641 tgggtgaggc aaatagcgta gcggcgtaat tttgctcgac acagcgaccg ttttgcatta
    11701 ccgccacgcg gtgggccagt tttctgacaa tgctgaggtt atgagtaata aacagcatgc
    11761 ccatattcag ctcgccttgc agttcgcgca acagctgtaa aatctgcgcc tggacagaga
    11821 cgtccagtgc ggtggtcggt tcatcggcaa ttaataattc cggtcgcgtt aacagcgcca
    11881 tcgcaatcat cacccgctgg cgttccccgc cggagagctg atgcggataa tctgtcagcc
    11941 gttttgccgc ctggcggata ccaacgcgat caaggcagtt aagaatttcg ccacgagccg
    12001 cttcccgacg catcccgcgg tggagtgaaa gcacttcata aagctgtttt tccagggtat
    12061 gcaatggatt taacgacacc atcggttcct gaaaaatcat ggcgatctta ttaccgcgta
    12121 caccgcgcaa cgtttgatcg ctggcgtgaa gcagcgattc gccatgaaaa cgaatatcgc
    12181 cggagagata ttcaaccggc ggggaaggga gcaggcgtaa aattgacagc gcggtaacgc
    12241 ttttgcctga acctgactca cccaccagcg ccagcgtttc gccagcctca atctgtagtg
    12301 aaacatcatt gactactgta cgtacggttt gctgatggcg aaaacccacc gacaaatttt
    12361 caatcgctaa cagagtttgc gtcatgctac accgccttat taggatcaaa tgcgtcgcgg
    12421 acggcttcac caataaagat cagcaaagac aataatatcg ccaccgacaa gaaggcggtg
    12481 atcccaagcc acggggcctg aaggttattt ttcccttgta acagcagttc accgagtgac
    12541 ggtgaaccga gcggtagacc gaagccgagg aaatcgagcg aggtcagggt ggttatcgaa
    12601 ctacataaaa taaacggtaa aaaggtgagg gtcgcgacca tggcattagg caacatatga
    12661 cgcaggatga tactgcgatc gctgacgcca agcgcctgtg ccgcacgaat gtagtcgaaa
    12721 ttacgagtac gtaaaaactc cgcccgcacc acgccgacca gactcatcca gccaaacaag
    12781 acagtaattg ccagcagcca ccagaagtta ggctgtacga cgctggaaag taaaataatc
    12841 aaaaacagcg tcggcatccc cgaccatact tcaataaagc gttgtcccca gagatcgact
    12901 ttaccgccgt aatagccttg tagcgccccc gccagcacgc ccatcacgct ggaacagaga
    12961 gtcagcatca ggccaaacag aaccgagatc cgcgtgccat agagaatgcg tgccagcaca
    13021 tcgccgccgt tggcatccgt tcccagccag ttttgccggg agggtggaga agggaagggc
    13081 ttatcggtag caaagttgat actggtagca ccaaagcgaa tcggtgccca cagtacccag
    13141 ccgttatttt ccagccgttg tttcagccac gggtcctgat aatcagcctg acttgccagc
    13201 gggccgccaa aatcgctttc gctgtagttt ttcaacaacg ggaaatacca actgccgtca
    13261 taacgtacca gcaacggttt atcgttggcg ataagttcag aacacaaact caaaccaaac
    13321 aaaacgagga aaatccataa cgaccagtag ccgcgacggt tatggcgaaa acgcgcccaa
    13381 cgggcctgat tgacggggct gagtcgcgac attaacgtcc ctcaaaatct atacgcggat
    13441 cgaccagcgt atagctgata tcactgacaa tattcagcag caggccaatc agggtgaaaa
    13501 tatacaaggt accaaacatt acaggataat cgcgcgagac ggtcgcttcg tagcccagta
    13561 agccaaggcc attgagtgaa aacatcactt caatcagcag cgagccggta aaaaacatgc
    13621 tgataaacgt cgccggaaaa ccggcaatca ccagcagcat ggcgttgcgg aacacatgtt
    13681 tccagagaat atttttttca cttacccctt tcgcacgcgc ggtcaccacg tattgcttgc
    13741 gcacttcatc aaggaatgag tttttggtca gcatggtcag cgccgcaaag ccaccaatca
    13801 ctgtcgccag caccggcagc gtgatatgcc acagataatc ggtgattttc tgataccacg
    13861 gcagcgaatc aaagttagcg gaaaccaggc cgcgtagagg gaacaggtcg aaatagctgc
    13921 cgcccgcgaa gaagacaatc agcaggatgg caaacaaaaa ggccggaatg gcgtagccga
    13981 tgatgataaa tgcgctactc cagacgtcaa agcggctccc attataaaca gctttgcgaa
    14041 tgcctaacgg aatcgacacc agatagataa tcagcgtgct ccacaatccg agggtgatgg
    14101 aaaccggcag actgtcttta atcagcgtca gcaccgaggc gctgcgaaaa aggctatcac
    14161 caaaatcaaa gcggatgtag tcccagagca ttttgaagta acgttcgtgg atcggcttat
    14221 cgaaaccgta gcgatgagtg atctcggcga tcacttctgg atctaatccg cgtccgccac
    14281 ggtaattact gtcgctgata ttgccgacgc ccgtttgcgc atggctggcg cgaatacctt
    14341 caccgcctgc gccgggtaat actccggcat taccaaactc aatggcggcg atggcctggt
    14401 cgaccggacc gccaggcgca atttgcacga tgaaaaagtt gatagtgata atcgcccata
    14461 acgttgggat caccagcaac agacggcgaa tcagataagc gcccatctac tctccctgtt
    14521 gtctggctga cggcagtttg gccgctttat taacgtcata ccaccaggta tcgataccca
    14581 ggctgtaaac agggcgtaca gcgggttggg agaatttatc ccaccaggcg agacggtctt
    14641 ccgccatgta ccacattggc agcatgtaat aattccacgt taatacgcga tccagtgctc
    14701 gccctaacgg cagtaatttt tctttatttc cctgcgcggc aataatttga ttgatcagcg
    14761 agtcgataac cggactttgc acgccggggg cattataagt ggaattgata tattccgatg
    14821 accaggaaat ctgtaaatcg gaactgggcc acggcatcgc ccgccatacg cgcggcatca
    14881 tgtcatagtc gcgactgcgc atgcggttgg tgatttgcga gttatccacc ttgcgaatgt
    14941 ccatgttgat acccagccgt tgcaggctgt gctggaacgg caatacccac tgactattgc
    15001 tgcttgcggg aagcaataat tcaaagctga gtggctgacc cgttgtggca ttaacgcgtt
    15061 gctgaccctt cagcacccag cccgcttcgt tgagaagttt gtcggctttt aacaggttgt
    15121 cacgatcgta gccatcgcct ttagataccg gcggctggta gatttgtgtg aagacttctg
    15181 gcggtagatc ttttttcatt ggtgccagaa gcaccagctc cgcggcgtcg gggtaatttc
    15241 tggccgcgta ttcggtattc tgaaaataac tgttcgtgcg actccaggcg ttgtaaaaca
    15301 acgccttgtt catccattca aagtcaaagg cgagggtgat tgcttcccgg acccggcgat
    15361 cgctgaatac cggacgttgg atattaaacg ccagccagcg cgtatcctgg gctgattcgt
    15421 tcttttgctc atctttgatg atgtattttt tatcgaaatt tttaccggta taacgcgtgg
    15481 cccagttttt agcgtcgttt tccatacgca aatcaaaggc acctgcttta aacgcttcaa
    15541 aggcgacatt atcatcgagg taataatcgt agcgaatggt gtcgaaattc cagcgtccac
    15601 ggtttaccgg taagtttgct gcccagtaat ctttcacgcg ggaatagaca atattttgcc
    15661 ccattttcca ggacgtaacg cggtacggac cactggcaag cggaggcgtg gcgagcgggt
    15721 cgctaagttt gtgatccttc cagtactttt ctggaaatac cggcagcgaa aacagactca
    15781 gcatatcttc tttgctggct ttagctaact caatgcgcac ggttaacggt gcaatggctt
    15841 tgacggtggt gcctttgtag accagacgaa attgcggtac gccttcggtc ataaattttc
    15901 gaaaagtaaa ctctacatcg cgggcagtaa tgggcgaacc atcatgaaaa cgggcgcgcg
    15961 gattaatagc gacctccacc caggaataat cgtcagcata gcgtgcgctt tcagcaatca
    16021 gcgggtaata actgcctggt tcgtcatcgg aagtcgtaaa tagcgtgtcg tacagctgct
    16081 cggtgcgtgc gcccgggttg ccgcgcagtg catagcggtt gaaattatcg aaggtgccga
    16141 gggctgacaa cgttatctgc ccaccttttg gcgcggcggg gttcacataa tcaaaatggt
    16201 tgaaattaaa cgcgtaccgg ggttcgccca gcacggcaaa ggcatagctt tccttgatag
    16261 cctgcgcctg cgcaccaaag gtgaacagag cgataaacag cagcagtatg cgcacaatca
    16321 tctgcaacgg atttcctttt actcatcatt agccgcaatc cggcatgctt tcactatgaa
    16381 taataaggga cgtaagcaca ccttaccact gcggcgtata cggtttcttc agccagcgaa
    16441 caaaatcgtc cagcggtaac gggcgactaa tccagtagcc ttgcatgaaa ttaacgccgc
    16501 gttcgcttag ccatcgcgct tgttccggcg tttcgacccc ttcagcaacc gtcagcatat
    16561 tgaggcgttt cgccagcgtc agcaccgcgt caagtacggg tgaagtgatc gtttccgtac
    16621 cgatggcgtt gataaatcca cggtcaattt tcagataatc gagcgtaaaa cgctcaagat
    16681 agataagcgc gctgtgcccg gtgccgaagt catcaatagc aatttctacg cctaccgagt
    16741 gcagccaggc gaagagttgt gtggcttctt gctctttcag catatcgcgc tcggtaattt
    16801 ccagcacaat ctggaaatgg tgtgcgggta gggaagtgag cagtttctgg atatctgctt
    16861 taaagctttc gctgtgcaga tggtccggcg caatgttaat gccaaatttg acgcctaccg
    16921 gcagcacttt ttctaattct gcggcatcgc gggcaattaa ctcaaacaga tgctgagtca
    16981 gcggcacaat catcttttgc gattcggcaa agttaatgaa ggcatccggg ggaatttctc
    17041 ccgcgacagg atgccgccag cgtagcagta cttccaggcc cgttactcgc aaagcttgtg
    17101 tatccaccac cggttgatac gccacgtaaa attgttcgcg cttgatggcg gtcatgattt
    17161 ctctgccggg gcgcatacgt acgctcatca ggtaatagca gagcaggcca acgacagttc
    17221 cactcatgcc gcccagtaaa aatgcgtacc acacatcgtt ccatgtccag tcatctgcat
    17281 aaagccgaac ggtcagagga atgcccgcaa ttttagtttc acggactggc atgtcggtta
    17341 attcgttaac gttcatcaaa cgtgaagaaa aggtagatag cgcagtattg ccaataatga
    17401 gggcgacgcc atcgtaatct tcctgccgtg aactataaaa gagtgaaggc gtcaggttga
    17461 gattcagagc ggcaaagacg ccgctatttt tcagcaaagg gttgcgatac cagatgacga
    17521 ttgcgggttt gttcggcacc atcggcgtgc cgggtaagat cgccatatcg acatttttat
    17581 taatgtcgag cgccggaatc aattcattga gtggaatgtc catctcaccg gtcgcagatg
    17641 aacagaatgt ttttttatct ttcaccagca caaacgtacg gacattcatg ctaaacgccg
    17701 cgcgggcggt cagttcgggg ttagcctgct ggcaggtatc taaggtcagc ggctggagcc
    17761 ggtcagtagt ggatttcagg tcggcaaaat aggtatcgag atacttttgt acgtcggtaa
    17821 tcagtgtgtc gtatttgact tcgtgcttat gccaggccac taaaaactga aggcaactca
    17881 ccagtatcgc aatcatcacg cctgcaacaa tgcaggtaag caggagctta cgtccagaat
    17941 tgggagcgcg tatgaacatg attccgttaa tttcctgaaa ttacgacaac atttaaaaga
    18001 taacaatgac cggactatag cctggatcaa gacgcggaaa catcattttc tctgatgaat
    18061 gaaaacaaaa aagcactgcc taagcagtgc tttcagttaa ctcgttagga tagccaaggg
    18121 attgcatcca aacggtttat tagctgcggc tgagaacccg gcgtgcttcg ttgtaacgct
    18181 tcttccagta cggttcattc atgctggaaa taataacacc actgctggtg gaagcatgga
    18241 caaactgatt gttgccgata taaataccga catggcgtcc cgttgaaccg gcacggaaca
    18301 gaactaaatc acccgtacgc aaattactgc gggaaacaga tttacccatt tcctgctgtt
    18361 cgtaagtcga acgtggaagt tctaagccaa attgctcacg gaatgtacgc tgtacgaaac
    18421 cagaacaatc gatacctttt ttagtgctgc cgcccagacg ataacgtacg cctttccagt
    18481 cagcatactg atccataatt cgcgatttta cgtcgacatt acgaaccagg ttttcaaatt
    18541 catcctgaga agcttgcagt gatgatgttt cactacccac tacacgtgtc tcaggatgca
    18601 tattctttgc ggtgttattt gcactacatg cagaaagcag aaccgctact gcaatcgcgg
    18661 ggatcccgcg caagatatat ctcaaaatcg gttgagattt gaccatgttg tttgttttcc
    18721 cttgaagtcc ttaacgacaa atatcgttat aaaaaaatgc caagcgacac gagactaatt
    18781 acctgggcat gttgaaacaa ttccttaagg ccaaatctgt ggactaacgc acaaaattat
    18841 atgctgtgag gataatctac cgccgaggca aaacgagttc gagattaccc gattggtgcg
    18901 gtcttggcga gcggttttac atgatttttt ataagaaaaa gtgatacaga aagttaataa
    18961 gcgggattgg tcaattaaag tacgattgag tgaaacgcat gcaatccatt catttactta
    19021 gagaatgatt tttgcgcgcg atggatgaaa gcacggtgcg catcatcagg attatcctca
    19081 ctataaaaat aaacctgatg atgttaatta ctgtgagtta tttgttttgg aaatgtttgt
    19141 tttttcccgg tagtgatttg tcaaaaaagg tgattaatcg atcacttaat ggcgtcagca
    19201 acacccaggg caaaccgatc aatatcacgg tcatcgaacc gacaatgatg tcagtaaacc
    19261 agtgtgcgcc aatcattact ctgggaaacg caaaaaccac aaaaataata agggcgataa
    19321 gacctgcaac tttgccgaaa taacgccaca tgaatgccga aaaaataagc agcatcatgc
    19381 cgtgatcgcc gggaaagcta tctcgtgagg catctttcgt gggaacagag agcagttcgc
    19441 tgacgcggtt aatgtcggta aaagtcaatg ttgggctggc ccgttttaca ggaattaatg
    19501 cctgacccag ctggtttaat accactgcag ttaatagcat gaccagacca ataatcacga
    19561 tacgtcgtct gcctggggcg ttttctttca gccagaaact cagcatcaac atacccatcg
    19621 ccagcagtga acaaccgtcg aaggcgcgat tgttggtcaa tgcaaccagc cacaaaaagg
    19681 ccttactttc gaccagtttc tgattaaaga aataaaaaat atccgcatca atcggcaacc
    19741 agaatccatg ataaacggga atataccagg aaagaaacag cgcgaggccg acaatattca
    19801 acaacactat ttgcggcaaa tttttaatca tattttctct aattatctta ctgaaaaacg
    19861 caggcaacct tacgcggtag tcgctaaacg aagcttcaac agcgcgctct gcaaagcatt
    19921 ccagtcagct tcgctgctgg aaatcagctc aatacgactg tccggtggcg caacgttttg
    19981 cgtttcaatg tgcaggtcat cgccctgacg gttgattcgt accagccctt cgggaatacg
    20041 cagcacgcct ttgacgcgtt ccaccggtgc aagtcgcgcc cattccagaa tgccaatggt
    20101 gtcgaatacc gtatcagcgt cgaatatcca gccgcaggcc tgatatcctt gcccgctgtt
    20161 cagactgcga cgccagcgtt gatgctctgg caggcttaac gctgctaacc cttttttcac
    20221 gacatgctga tgagaatgcg cggcgctggc gggcaactcg gctaaattgc gacgcggcaa
    20281 atccagaaga tgaccgtcaa ctttcccatg ctcactgtga attaattgtc gatcgccacc
    20341 attttgctgc caccaacgct gtagcgcttg ctcactttcg ggcgtcgcac ggtcggattt
    20401 attggcgaca atgatgtctg cggcagccag ctggtcacgg aagttttcat tgctggcgct
    20461 tttttcgtcc agcagcaagc gcggatcgag aatgcacaag gtggcgcgca gatctatcca
    20521 cggttcatag actggtgcgg ttaacagatc gaggatctgt ttcggatggc ccagcccggt
    20581 cggctctatc aacaagcggt ctggttttcc ctgacgcagt aaggtattca accctacctg
    20641 catgggtaaa ccattaacgc agcacatgca gccgccgggg atctctttca gcaatgcgcc
    20701 gctatcggcg agcaaagcac catcaattcc gacttcccca aattcattaa ccagtaccgc
    20761 ccatttttcg ttgggatctt tatgggctaa cagatgaaga atcgacgtgg ttttcccgct
    20821 gccgagaaaa ccggtgatga ggttggtcct ggtcacaacg gctccgcaat actctggtaa
    20881 gttaactgag cgttaatcct ggctaaaaaa ggcagaaaag agaaggggag agcggacgaa
    20941 ggtgagcttc atccgcttta accgcagaaa tcgttaaatt ttgagggtgt ttaatagcgc
    21001 ctgatgtgcg ccgaattgta cgatttgttg ccaggcttgt tcgatggcct gcacaaaatg
    21061 cgggttatct ggcagatcat caccgaaaac ttcacgcagg gaaagcaggg cggttacgcg
    21121 ttgttcactg ctgctgcccg caacaagttc gcgaatttta tcgctaagcg gatcgcgaac
    21181 atcaatggca tttccggcat catcaacgcc gctgacgtaa cgcatccagc cagcaacgcc
    21241 taatgccagc aacgaccagt ccgtttcgca ccccagatgt atgcgaatac ctgccagcat
    21301 acgttgcggt aatttctggc taccatccat tgcgatttgc caggtcttat gtttcagcgc
    21361 cggattagca aaacgtgcaa ttaacttgtc cgcatattgt gttaaatcga catctttaat
    21421 ttgtagtgtc ggcgcttgct cattcagcat taatgttctg gcggcatggc gaaatgcgcg
    21481 atcctgcatg caatcactga tatgggcgaa tcctgaaagg taacccagat aagcgagaaa
    21541 agagtggctg ccattaagca tacgcagttt catctcttcc catggcagga catcattcac
    21601 catttgtaca cctgcgactt cccaggcagg acgcccagcg acgaagttat cttccactac
    21661 ccactggata aacggttcgc agctaatcgc gcagggatca tttaccccca gatgctggct
    21721 gatttctgcc agtgattcgt cggttgcagc cggaacaatg cggtcgacca tggttcccgg
    21781 aaaactgacg tgttctttta tccacccggc gagttctggc gaacgttttt ctgccattcc
    21841 cagcaccgcg tttttcacca catggccatt gtcgggaata ttgtcgcagg agagcacggt
    21901 aaacggtgta aggccgcgct cacggcggcg tttcagtgct tcgacgagaa tacccggtgc
    21961 ggagtgaggt tcttcagggg tttgtagatc gtgaataatc cgcggattac tggtgtcgag
    22021 tgcaccggtg gccgggtcaa tacaatagcc tttttcggta atcgtcaggg aaacaattgc
    22081 cacctgtggc tcgcaaaatt tctcaataat tgccgctaag gaatccagtt ttgcattaag
    22141 gcattcgtga acggcaccga caattatcac ctgattgccg tccgcacctt tttccagcac
    22201 ggtatataaa tggttctgtg cgcggagctg gctcatcagt tgatcaccgc tgaacaagct
    22261 gatttcacag atcccccagt cgccgccctg ggcattcagc acacgatcgg tcagtaacgc
    22321 ctgatgagcg cggtgaaagg cgccaaagcc aaaatgaacg atacgtgatt gcaactgctg
    22381 gcgatcgtag cgtggagcgt gtacatgatg cgggagcgta acggaggcaa ttgtcttcat
    22441 aggagtcacg tcttagtaac gataaattaa caatgacagt gtaaagttat atgacagcaa
    22501 ttgaaattgg tctggattgt taatgcgatg aagtgtgagg gcgatcaatc aacgagtgaa
    22561 aattgagggc gtaaggtgtt caggaagcgc aaaatagaag attggtataa taaattaaaa
    22621 aagggacagc ctgagctatc ccttttctgc atgcggctga agttagtcag cacgccccat
    22681 ataacggcgt tcttcgatat ggatacgaat tttttcgccc gggcttaagt attctggtac
    22741 ctgaatcacc agaccagtgc tcaatgtcgc cggtttgtta cgggcgctgg cagatgcccc
    22801 tttgatgcct ggtgcggttt caacgatttc cagatcaacg gtctgcggca gctcaagcgc
    22861 cagcagttga ccgtcccagg tcagcacctg catgtccggc atgccgccct ctggcataaa
    22921 cagcaactct tcttcaatct gatctttggt gaaggtatac ggggtatagt cttctttatc
    22981 cataaagaca tattcgttgc catcgacata ggagaagtca acgtaacggc gggtcagcgt
    23041 cacggtgtca acgatatcat cacctttgaa acgctcttct actttcagcc cggtacggac
    23101 atcagaaaaa cgcattttgt acagcgtagc ggcaccgcgg gcagtgggcg actgaatatc
    23161 aatatcctta accagcagca gtttgccgtt gtaattcagt accatacctt ttttaatttc
    23221 gttcgctctt ggcatcgaaa aattcctgta gtcgaaaatg tcaaaaatat cgcgacaaag
    23281 tactcgcgac gggcctttcg ggcaagtggt attcgcactt ttgctggtgc aaaaaaggtg
    23341 gtactgtgcg cgctcatcaa tccggtggtt aaccttaaga gaacaacgta tgaaatgccg
    23401 tccgggttgc ggggcgtgtt gcactgcccc ttcaatttcc agccctattc ccggtatgcc
    23461 agatggcaag cccgccaata cgccctgcat tcagcttgat gaacagcaac gctgtaaaat
    23521 tttcacctcg ccactgcgac cgaaagtctg tgcaggctta caagccagcg ctgaaatgtg
    23581 cggtaactcg cgtcagcagg ccatgacctg gcttatcgat ctggaaatgc tgaccgcgcc
    23641 ttaaacatct ttaatccgca gtaagcaaaa cagagtggcg ataatcatca ccatcgcaaa
    23701 ccagaacaca gcgtgataat tccagatctc ggcgacgatg cccgccactg atcctgcgat
    23761 gatccagccc acgcgcgacg tgttggtata gagcgtggtg gctgaacccg cctgaccggg
    23821 catcagatcc tgaaaataga gcatcccgat gccgcccaga atgccaataa aaatagcatt
    23881 tagcagctgc aagcccaaca gaatgacagg tgaatgcgcc atcagcattc ctgcgtaaaa
    23941 acagacgcca cccacggcag caacgcgcat taagaaacgc ttacccagac gtttggcgaa
    24001 atatccggca atcaacatcg tcgggatttc cagcccggcg gcggtcccca tcatcacacc
    24061 ggccagtttc tcgggaagat gcagttcgtt gataataaat agcggcatgt tgatgatgta
    24121 caggctgttc gagccccaca tcaatgtaca aatgacaaac agcagcagcg tatcgcgacg
    24181 gttacggcgc ggcgcttcga tcgtgccggt cgccagcgga agctcttttt gcatcgacgg
    24241 taaaaacagc cacaccatca caccgcaaac aataaacgct accgctgcgc tcagatacat
    24301 taccgtaaag ctgaaaccca tcgctaaggc ataagccagc ggtgggccaa tgacccatgc
    24361 cagtgaaacc tgagcgcgta aaaaagagct gaacatcacc gcctcacgtc cggttttgtc
    24421 ggcatgttca cgggcaaggg caaacatttg cgggttagcg gtcgagccaa agctgctaag
    24481 aaagacgcca acgaatagca aaacaaagta gttgcgattc caggcaaaaa gggtgcaggc
    24541 cagcacgcct aacaggcagc aaaagacaat cagcgatttg cgatcgccgc gcttatcaga
    24601 gcgcccggcg agaaactgac ttaccagaat cccaatgaca gcgctgccgg taaagaagaa
    24661 tcccaccatc gccggacgag catgtacttc atcagtaaga aaaatactga gtgtcggggt
    24721 ttgcagagcg cccgcaatac cggtgagaaa ggcaacgatt aaaaacgccg tcgaggtcag
    24781 gtcaaacgat ttcgcgctgg agactgcggg ggagttatgc atgctggtcg atcactggaa
    24841 gaggaagacg cggagtttac gctgcttgtc agaaaataaa cagacagcga aatcaaaagc
    24901 gccacgaata atcaaaatgc catttcactg ttgctgattg gctgctgacg ctgctgaaat
    24961 gaaattgctg ataacggatt ttcccatcag caattaggaa aaatggcaaa aaattgtgca
    25021 gcacatcaaa cttttgctca taactttacg gctttccttg cgtgctgaaa cagaagtatt
    25081 atgctttctt gaaacgtttc agcgcgatct tgtctttaac cctaagccag gttggcgctt
    25141 tttttctcat agaggctgaa tcgtttcaat tcagcaagag aggagaacta tgttccagtt
    25201 atccgtacag gacatccatc cgggcgaaaa ggccggagac aaagaagagg cgattcgcca
    25261 ggtcgctgcg gcgctggtgc aggccggtaa tgtagcagaa ggctacgtca atggcatgct
    25321 ggcgcgcgaa cagcaaacct caacgttcct cggcaatggt attgctattc cacacggcac
    25381 taccgacacc cgcgatcagg tgctgaaaac cggcgttcag gtatttcagt tcccggaagg
    25441 cgtcacctgg ggtgacggtc aggtagcgta cgtggcaatc ggtattgctg ccagctcgga
    25501 tgaacatctg ggcctgctac gccagctgac ccacgtactg agcgatgatt ccgttgctga
    25561 acaactgaag tcagcaacaa cagcagaaga acttcgcgca ttactgatgg gcgaaaagca
    25621 gagtgagcag ctgaagctcg acaacgaaat gctgacgctg gatatcgtcg ccagcgatct
    25681 gctgactctt caggcgctga acgctgcgcg tctgaaagag gcgagggcag ttgacgccac
    25741 tttcgtcacc aaagccatca atgaacaacc gctgaacctc ggacagggta tctggctgag
    25801 cgatagcgcc gaaggcaatc tgcgtagcgc gattgcggta agccgtgcgg caaatgcttt
    25861 tgatgtggac ggcgaaacgg cagccatgct ggtgagtgtg gcgatgaatg acgatcagcc
    25921 catcgcggtt cttaagcgtc tcgctgattt attgctcgac aataaagctg accgcttgct
    25981 gaaagcggat gcggcaacgt tgctggcgct gctgaccagc gatgatgcgc cgaccgacga
    26041 cgtgttaagc gcggagtttg tggtgcgcaa tgaacacggc ctgcatgctc gtccaggtac
    26101 catgctggtc aataccatta aacaatttaa cagtgatatt accgtgacaa accttgatgg
    26161 taccggcaaa ccggcaaacg gacgtagtct gatgaaagtt gtggcacttg gcgttaagaa
    26221 aggtcatcgc ctacgcttta ccgcccaggg tgcagatgct gaacaggcgc tgaaagcaat
    26281 cggcgacgct atcgctgctg gtcttgggga gggcgcataa tgagcagacg tgttgctact
    26341 atcaccctta atccggctta tgaccttgtt ggtttctgcc cggaaattga acgcggcgaa
    26401 gtgaacctgg tgaaaaccac cggtctgcat gcggcgggta aaggcatcaa cgtggccaaa
    26461 gtattaaaag acctgggaat tgatgtcacc gttggcggct tcctgggtaa agacaatcag
    26521 gatggttttc agcaactgtt cagcgagctg ggcattgcca accgtttcca ggttgtacag
    26581 gggcgcaccc gaattaacgt taagctgacg gaaaaagacg gcgaagtgac cgacttcaac
    26641 ttctcgggtt ttgaagtcac ccccgccgac tgggaacgct ttgtgactga ttctctgagc
    26701 tggctcggtc agttcgatat ggtctgtgtc agcggaagct taccgtcagg cgtcagcccg
    26761 gaagcgttca ccgactggat gactcgcctg cgtagtcagt gtccttgcat tatctttgat
    26821 agtagccgtg aagcgttagt agcaggtttg aaagcggcac cgtggctggt gaaacctaac
    26881 cgccgcgagt tggaaatctg ggcaggccgt aaactgcctg aaatgaaaga tgtgattgaa
    26941 gctgcacatg cgctacgtga acaaggtatt gcgcatgttg ttatttcact gggtgccgaa
    27001 ggcgcgcttt gggttaatgc ctccggcgaa tggatcgcca aaccaccgtc agtcgatgtc
    27061 gtaagcaccg ttggcgcagg ggattctatg gttggtggcc tgatttatgg cttgctgatg
    27121 cgtgaatcca gtgaacacac actgcgtctg gcgacagctg ttgcagccct ggcggtaagt
    27181 caaagcaatg tgggtattac cgatcgtccg cagttggccg caatgatggc gcgcgtcgac
    27241 ttacaacctt ttaactgaca gcaggagagg cataatgaaa acgctgctga ttattgacgc
    27301 taatctcggt caggcacgcg cctatatggc gaagaccctg ctgggcgcgg cggcgcgaaa
    27361 agcaaaactg gaaatcatcg acaatccgaa cgacgcagaa atggcgattg ttctcggtga
    27421 ttccatcccg aatgacagcg cgctgaacgg taaaaatgtc tggctgggtg atatttcccg
    27481 ggcagttgcg caccctgagc tgttcctgag tgaagccaaa ggccatgcga aaccttacac
    27541 tgcgccggtc actgcgacag caccggttgc cgccagcggt ccgaaacgcg tagttgcggt
    27601 gactgcttgc ccgactggcg tagcacacac ctttatggcg gctgaagcca ttgaaaccga
    27661 agcgaaaaaa cgtggctggt gggtgaaagt tgaaacccgt ggttctgttg gcgcgggtaa
    27721 tgcaatcact cccgaggaag tagccgcagc ggatctggtg attgtggcgg cagatatcga
    27781 agtggatctg gcgaaatttg ctggtaaacc gatgtatcgt acctctaccg gtctggcgct
    27841 gaagaaaacc gcgcaggaac tggataaagc ggttgctgaa gcaacgccgt atgaaccggc
    27901 gggcaaaact caaacggcga ccactgaagg taagaaagag agtgcaggcg cttatcgtca
    27961 cttgctaacg ggcgtctctt acatgctgcc gatggtcgtt gctggtggtc tgtgtatcgc
    28021 gctttctttt gcttttggta tcgaagcgtt taaagagccg ggtacgttgg cagcggcgct
    28081 gatgcagatt ggtggtggtt cagcctttgc gctgatggtg ccggtactgg caggttatat
    28141 tgccttttcc attgccgatc gtccgggcct cacgccgggt ctgattggcg gtatgctggc
    28201 ggtcagcacc ggttctggct tcattggtgg tattattgcg ggcttcctgg ctggttacat
    28261 tgcgaagtta atcagtacgc aattgaaact gccacagagt atggaggcgc tgaaaccgat
    28321 cctgatcatt ccgctaattt ccagtctggt ggtcggtctg gcgatgatct acctgatcgg
    28381 taaaccggtt gctggcattc tcgaagggtt gactcactgg ctgcagacca tggggactgc
    28441 gaatgcggtt ctgctggggg cgatcctcgg tggcatgatg tgtactgaca tgggcggtcc
    28501 ggtaaataaa gcagcgtacg cattcggtgt gggtctgctg agtactcaaa cctatggccc
    28561 gatggcggcg attatggcgg caggtatggt gccaccgctg gcaatgggcc tggcaacaat
    28621 ggtggcgcgt cgcaaattcg acaaagcgca gcaggaaggt ggcaaagccg ctctggtact
    28681 gggactgtgc tttatttcgg aaggtgcaat tccgtttgct gctcgtgatc cgatgcgtgt
    28741 gctgccgtgc tgtatcgtgg gtggggcgct gactggcgca atctcaatgg cgattggtgc
    28801 gaaactgatg gcaccgcacg gtggtctgtt tgttctgctg atccctggcg ctattacgcc
    28861 ggtattgggt tacctggtag caattattgc cggtacgctg gtggcgggtt tggcctatgc
    28921 cttcctgaaa cgtccggaag tggacgcagt agcgaaagca gcgtaataaa aggtgtgtta
    28981 cagggcagaa atttcctgcc ctgtatcatt ggatgagcgc aaagggaatg accggtttgt
    29041 aaatgtaatt ttatgaattt aaatggtttt atctttcagt agaaacctga aacgttcatc
    29101 aaacactaag gttatttcct caagggcaga ttattttctg ccctttttta gtgttcctgt
    29161 tctgtgcatc ccgtcacaaa ttcctcgctt ttgtaattca ggcatttaat cgcattttgt
    29221 gagcctgctc aaatctgccc attttgcctc tctggcggta atttacttta cgtaaaacat
    29281 gcatactatg agcacatgtt taaatatgaa cacatgttta aaccgtcttt gcagtatcta
    29341 tattcttgtg gattgacagg gttggtaatt ttttaccaga ggacgtgcta tgcgcgaaaa
    29401 ggattatgtc gtaattatag gttcggcgaa tattgatgtc gccggatatt cacatgaatc
    29461 attaaattat gcggattcaa atccaggtaa aataaaattt acgcctggtg gagtagggcg
    29521 caatattgca caaaacctgg cgttgctggg taacaaagcc tggctactga gcgcagtagg
    29581 cagtgatttt tatggtcaat cgctgctaac gcaaaccaat caatctggcg tttatgtcga
    29641 taaatgcctg attgtgccgg gagaaaatac gtctagttat ttatcattac tcgataatac
    29701 cggtgaaatg ctggttgcta taaatgacat gaatattagc aacgctatta cagctgaata
    29761 tctcgcacag caccgtgaat ttattcagag ggcaaaggtc attgtcgcag actgtaatat
    29821 cagtgaagag gcactggcat ggattctgga taatgccgcc aacgtacccg tatttgtcga
    29881 tccggtttca gcgtggaaat gtgtcaaagt gcgcgaccgt ctaaatcaga tccacactct
    29941 caagccaaac cgccttgaag cggaaaccct gagtgggatt gcgctgtcag ggcgtgacga
    30001 tgtggcaaaa gttgctgcct ggttccatca acatggcctg aaccgactgg tattgagcat
    30061 gggcggcgat ggcgtttatt acagcgatat cagcggtgaa aatggctggt ctgcgccgat
    30121 caaaaccaat gttattaatg ttaccggagc gggcgatgcc atgatggcgg gacttgcttc
    30181 gtgttgggta gacggaatgc cgtttgccga atctgttcgt ttcgcacagg gatgttcgtc
    30241 aatggcgctc tcctgtgaat acaccaataa ccccgattta tcgattgcca acgttatatc
    30301 gttagtggag aacgcagaat gtctgaatta aaaatttccc ctgaattatt acaaatttcc
    30361 ccggaagtgc aggacgcttt aaaaaacaaa aaaccggttg tggcgctgga atcgaccatt
    30421 atttctcacg ggatgccgtt cccacaaaat gcccagaccg caattgaagt agaagaaact
    30481 attcgtaaac agggcgcagt acctgccact atcgccatta ttggcggcgt gatgaaagtg
    30541 ggtttaagca aagaagaaat tgaattactg ggtcgtgaag ggcataacgt gaccaaagtt
    30601 agtcgtcgcg atttaccttt tgttgttgcc gccggaaaaa atggcgcaac cactgtggct
    30661 tcaacgatga ttattgcggc gcttgccgga attaaagtat ttgccaccgg gggaattggt
    30721 ggtgtgcatc gcggggcgga acataccttc gatatttctg ccgatttgca agaactggca
    30781 aatactaatg tcaccgttgt ttgtgccggg gcgaaatcta ttctcgattt aggattaacc
    30841 actgagtatt tagaaacctt cggtgtgccg ttaattggct atcagactaa agcgctgcct
    30901 gcgtttttct gccgtaccag ctcgtttgac gtcagcattc gtctcgacag cgccagtgaa
    30961 attgcccgtg caatggcggt gaaatggcaa agcgggctga acggtggcct cgtggtagcg
    31021 aacccgatcc cggaacagtt tgcgatgccg gaggaatcta tcaatgcagc catagatcaa
    31081 gccgtcgccg aagccgaaga gcagggcgtt attggtaaag aaagtacacc gttcctgctg
    31141 gcgcgcgttg ctgagctgac cggcggtgac agcctgaaat ccaatatcca gctggtgttc
    31201 aacaacgcca ttctggcgag tgaaattgcc aaagaatacc agcgtctcgc gggttaattt
    31261 ctgacgggca gaaagccgtc gcccgtactc atcaagcata ccaaaacctg gcgctgacgc
    31321 cgggtttact ggcatgaagg gaaaatcatt atggatataa tgagaagtgt tgtggggatg
    31381 gtggtgttac tggcaatagc atttctgttg tcagtgaata aaaagagcat cagtttgcgc
    31441 acggttggag ccgcactgct gctgcaaatc gctattggtg gcatcatgct ctacttccca
    31501 ccgggaaagt gggcagtaga acaggcggca ttaggcgttc ataaagtgat gtcttacagt
    31561 gatgccggta gcgccttcat ttttggttcg ctggttgggc cgaaaatgga tgtcctgttt
    31621 gacggtgcgg gttttatctt cgcctttcgc gtacttccgg cgattatttt cgttactgcg
    31681 ctcatcagtc tgctgtacta cattggcgtg atggggctgc tgattcgcat ccttggcagc
    31741 attttccaga aagctcttaa catcagcaaa atcgaatctt ttgtcgcagt cacaactatt
    31801 ttcctcgggc aaaatgagat cccggcgatc gttaaaccgt ttatcgatcg catgaatcgc
    31861 aacgagttgt ttaccgcaat ttgtagcggg atggcgtcca ttgctggttc gatgatgatt
    31921 ggttatgccg gaatgggcgt accaattgac tacctgttag cggcatcgct gatggcgatc
    31981 ccaggtggta ttttgtttgc acgtattctt agcccggcca ccgagccttc acaggtcaca
    32041 tttgaaaatc tgtcgttcag cgaaacgccg ccaaaaagct ttatcgaagc ggcggcgagc
    32101 ggtgcgatga ccgggctgaa aatcgccgct ggtgtagcga cggtggtaat ggcgtttgtc
    32161 gcaattattg cgctgatcaa cggcattatc ggcggaattg gtggctggtt tggtttcgcc
    32221 aatgcctctc tggaaagtat ttttggctat gtgctggcac cgctggcgtg gatcatgggt
    32281 gtggactgga gtgatgccaa tcttgcgggt agcctgattg ggcagaaact ggcgattaat
    32341 gagtttgttg cttatctcaa tttctcgccc tatctgcaaa cgagcggcac gctggatgtc
    32401 aagaccattg ccattatctc gttcgccctc tgtgggtttg caaactttgg ttctatcggt
    32461 gttgtcgttg gcgcattttc ggctatttcg ccaaaacgtg ctccagaaat cgcccagctt
    32521 ggtttacggg cgctggcagc agcaacgctt tccaacctga tgagtgcgac tattgccggg
    32581 ttctttattg gtctggcgta atgctcgatt tgtagaccgg ataaaacgta ttaagcgttg
    32641 catccggcaa tcgcgctatt gcaaataatt tttactgcat catcccggag aatttattct
    32701 ccgggtccat ctccagcgcc agtcctgaca actgctttct gtttttaatg agataccctt
    32761 tcttgctttt agttaataaa ccgtcgtgaa tgaattgcgc gagaacatat aaaagatgtc
    32821 gataagaaac gcctaaatac tctgccgctt gcgtatgctt ttcgtgataa agatcacctt
    32881 cctgcgagag taatataaaa gctgccaggc gattaactaa cggaaatgat tgattctgag
    32941 ttaaagaaac aatattacga taatttttat gacttaaggt gacgcagagt tttcgtaaaa
    33001 atagcgtgtc gtttaataac agcgggcggt aatgtttcat agggagcgca aggcaccaac
    33061 actcttcaat agcctgcacc gcacgcggtt catggtcttt gtcgattaac tcaatctcgc
    33121 caataaaaca gggggcggca aagaaatcga tcagcgacac gcgaccatta gctagcgtgg
    33181 cgtaaagcct ggcgcggcct cgcgtcaggt aaaacagcca ggacggctgt tgcccttcct
    33241 gcacaatgta gtcacgcgct aaaaaatgaa acagccgcgt atcggctgaa acatccgtta
    33301 aaaagcaatc cttaaacgcg gattcactca tcaattgctg cttaagatca ttattgtgga
    33361 tttctttcac gatgtttatc cgctatgaga tttctcatat ttagcatacg tacgcggttg
    33421 gtaagattca atcatcggcc cggtcgaatt ttctttgctg tagaaaaaat aacgcaactg
    33481 gaaacagagg aaataaacaa tggaaaagag aaaaattatt ctggattgtg atccgggtca
    33541 tgatgatgct attgcaatga tgatggcagc gaaacatccg gcaatagatt tattaggcat
    33601 cactattgta gcgggtaatc agacgcttga taaaacatta attaatggcc tgaatgtttg
    33661 ccagaaactg gagattaatg ttccggttta tgcggggatg ccgcaaccca ttatgcgtaa
    33721 acaaatcgtt gccgataata ttcacggtga aaccggactg gatggcccgg tattcgagcc
    33781 gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata ccctgatggc
    33841 aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg cggtggcaat
    33901 gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg gcggcgctta
    33961 cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc cggaagcagc
    34021 ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc tcaccaacca
    34081 gaccgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc ctgccggaga
    34141 gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact acggcctggc
    34201 tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg atggcattaa
    34261 aacacaggag atgtacgtcg aagtggacgt caacagtggc ccttgctatg ggcgtaccgt
    34321 ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca tcactattga
    34381 taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca aaacccatta
    34441 agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt tctgatgggc
    34501 agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca tgagaagtgt
    34561 tctgggaatg gtggtattgc tggcaattgc atttttgctg tcagtaaaca agaagaagat
    34621 cagcctgcgt accgttggcg cggcgttagt gttacaggtt gtgattggcg ggattatgct
    34681 ttggttaccg ccagggcgtt gggtcgctga aaaagtcgct tttggcgtgc ataaagtgat
    34741 ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc cgaaaatgga
    34801 cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg caattatctt
    34861 cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt taattcgcat
    34921 tctcggcggt atcttccaga aagcgttaaa tatcagcaag attgagtcat tcgtcgcggt
    34981 caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct ttatcgatcg
    35041 tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga ttgctggttc
    35101 gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg cggcatcgtt
    35161 aatggcgatc ccaggcggga tcttgtttgc ccgcctgtta agcccggcta cggaatcttc
    35221 gcaggtttcc tttaataacc tctctttcac cgaaacaccg ccaaaaagca ttattgaagc
    35281 cgctgcgaca ggggcaatga ccgggctgaa aatcgccgcc ggtgtagcga cagttgttat
    35341 ggcatttgtc gccatcattg cgttaattaa tggtattatc ggcggcgttg gcggctggtt
    35401 tggttttgca catgcctcgc tggagtccat tttaggttac ctgttggccc cattggcgtg
    35461 ggtgatgggg gttgactgga gtgatgcaaa tcttgccggg agtttgattg ggcagaagct
    35521 ggcgatcaat gaatttgtcg cttatctcaa tttctcgcca tatctgcaaa cgggtggcac
    35581 tctggatgct aaaaccgtgg cgattatttc tttcgcgttg tgcggtttcg ctaactttgg
    35641 ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg ccacaccgtg cgccggaaat
    35701 cgcccaactt ggtttacgcg cgctggcggc ggcgacactt tctaacctga tgagtgctac
    35761 tattgcagga ttctttattg gtttagcgta gtgcgcaatt tatgagccgg ataagatgcg
    35821 gtcaagcgtc gaatccggca acattgttac accattggca ctaatgaaag cgcgttatcg
    35881 gcagacaggg tagggttgtt taagctgccg ctggcgcgcg aaattgccgc gcaggctacc
    35941 gcaaaacggg cgctgtcgcg gaaactgtat ccttcaagaa agctatatac caggcccgcc
    36001 ataaaaccat cgtcagcacc aaaactgtct actgtcgtat gcgctggcgc ggtcagcaaa
    36061 aattgttctc catccttatc gctgcaaaat acagattcat ctttcagata aacaaaaatg
    36121 cgtttaacac cttgttgatg cagcgaattt acagctctga tgcgatcgtt gtcgtcttta
    36181 atcggttgcc cccacaaaat ttccagttca ttttgtgttg gtttcagagt gtggatccgg
    36241 ctgtaccagt tttttacttt gttcgcttta aattcggaaa cggtatcgac aaacatcgga
    36301 atttcatcag caatggtaaa gacccactcc agggcttcag gtgtgagatt acaatcagcc
    36361 agcaccacac cagcatggcg aatcaagtcc cgtgaagtat tgagcagttg aggcgttagc
    36421 tgttgcagaa tatgtgtgtc attgattgcc aggatagttt cttcctgctt attagcgata
    36481 gcgagataag ttgcagtgct gtgtccatgc agacgaatgc agtttgaaac gttcacgccc
    36541 gcgcggcgcg tttcttccag taatgtctcg ccgtaaaaat cgttgccaat agctgatatt
    36601 aaatgcacat cccgaccaag cagagccagg ttatgcgcga tattacgtcc gacgccgcct
    36661 gcggaacagt gaacagaacc tggatgagag gctgcttgcg ggtaacggat atccgccatt
    36721 ccgcgaatat ccatattgat tgcacccaca accacacagt aatcctgctc ggtaaggata
    36781 tatcctttgc ctttaatcaa acccttgcgc atcaaatcca taatatgagc ggcaacacgt
    36841 gagcggctga tttgcagaat atcggcgatt tcattctgtt gaatcagcgg gttacgccgt
    36901 aaaattttaa gaatctgctt ttccctgtcg ttcatcgatc aggccaccgc tttttcagtt
    36961 tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat ggtttcaagg
    37021 atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc atcatgaccg
    37081 atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt gcttttcgca
    37141 tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa atccgcgaac
    37201 gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc atggcaggta
    37261 tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc cgcgagatgt
    37321 tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac cgctgttaca
    37381 ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag gcaatcctct
    37441 tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag ccccagttgt
    37501 tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc ttcagtgacc
    37561 ggatgtccga ggttaatcag atagctgtcg tggggaagaa tttgcgccga tgtgtagtga
    37621 tatttttcac aggccgcttt gaattcatcg atggtttgcg tcgtgagcgg tgcggcacgc
    37681 cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc ggcggcgcga
    37741 attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta tttcatgcga
    37801 ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac aggagcaaga
    37861 tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac caccaacaat
    37921 cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt ttttcagcgc
    37981 gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga aggtatccag
    38041 cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa agatggcaac
    38101 gacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc cgctgttcgc
    38161 cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca gcatcatcac
    38221 gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg catgacctgc
    38281 cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga aagtttccgg
    38341 actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatag cgacggtccc
    38401 gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt tcactactgg
    38461 ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga tcaaccagct
    38521 ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca ggaaggtact
    38581 ggacagcgtc agcacgtcaa tgatgatccc actgataccg acatcggcaa tttgcgagaa
    38641 cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct tggcaaacag
    38701 cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta aaaccatccc
    38761 caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg gaatggaacc
    38821 accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa taaaatgcca
    38881 cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct ttaaccgggc
    38941 ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata attaatcttt
    39001 ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt gcagaagtat
    39061 tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa tcagcagtga
    39121 gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca actgtttgat cgcgtgggga
    39181 aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg gcattgcttg
    39241 aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt cgtatctatg
    39301 ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat cgccatgatt
    39361 atccgcagtt gccgattgaa cttagcgttg ggaacagcca ggacgtgatg caagcggtgc
    39421 tggatttccg cgttgatatt ggttttattg aaggaccgtg ccacagcact gaaattattt
    39481 ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg ccgttggccc
    39541 gtggtccggt caccttagag cagctggccg ctgcgccgtg gatcctgcgt gaacgcggtt
    39601 ccggcacgcg ggagattgtc gattatctat tgctgtcaca tttaccgaag tttgagatgg
    39661 cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg ttgggaatta
    39721 gttgcctgtc gcgacgggtg attgaagatc aattgcaggc aggcacatta agtgaagttg
    39781 cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg caaaaacacc
    39841 tttccaacgc gctacggcgc tttctggact attgcgatcc cgcgaatgta ccgcgttaag
    39901 ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc ctcagcatgc
    39961 attcgccaga aaagagattg gctgctttac ttataatccc tgggcgatca tgaaggtgtc
    40021 ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc gcgcctcatt
    40081 tttaagatgg atagcatttt tgtatggttt ccgaaactaa aaccacagaa gcgccgggct
    40141 tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta
    40201 caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc
    40261 tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc ggtgaactgg
    40321 ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat gttgaagaag
    40381 gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact atcgccgttg
    40441 acctggttgc agctcagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct
    40501 ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta catctcagtt cgtggctttg
    40561 gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg
    40621 ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact
    40681 ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga
    40741 ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc gagtccgaag
    40801 atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc ctgttgttct
    40861 atgtgttcgc gatcctgatt atcagcctga ttattccgta caccgatccg agcctgctgc
    40921 gtaacgatgt taaagacatc agcgttagtc cgttcaccct ggtgttccag cacgcgggtc
    40981 tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg tcagcgggta
    41041 actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac ggtaaagcgc
    41101 cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg tatgcgacga
    41161 cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg caaccagacg gtatacctgt
    41221 ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt gccattagcc
    41281 actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat ctgccgtacc
    41341 gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg attatcactt
    41401 tgggccagaa ctacgaagcg ttcctgaaag ataccattga ctggggcggc gtagcggcaa
    41461 cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg attaaaggaa
    41521 ctcacttcgt acgttacagc gaaatgaagt tcccgcagaa cgataagaaa taagtttcct
    41581 cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt ccctgacaaa
    41641 tcatgcggat ataaaattta acatttggat tgataattgt tatcgtttgc attatcgtta
    41701 cgccgcaatc aaaaaaggct gacaaatcag aggctgttcc ggctttctgg gatgatcacc
    41761 tgcatacaaa taagtccacc gcgatgctgc cgtacgcaag gggacgtgaa gaagatgtga
    41821 gcgataaccc attttatttt cgtagttacc tcatggagat atggaatgtt taggttgaac
    41881 cctttcgtac gggtcgggct gtgtttgtcc gctatttctt gtgcatggcc tgtgttagcg
    41941 gtcgatgatg atggcgaaac gatggttgtc actgcatctt ccgttgaaca aaacctcaaa
    42001 gatgcacccg ccagtatcag cgtcattacc caggaagacc tgcagcgaaa accggtacag
    42061 aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga aggggataac
    42121 cgtaagggcg ttagtattcg tggtctggac agcagctaca ccctgattct cgtcgacggt
    42181 aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg atttcgatct gaactggatc
    42241 ccggtcgatt ccatcgaacg tattgaagtg gttcgtggcc cgatgtcgtc gctatacggc
    42301 tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca gaaatggtcg
    42361 ggtaccgtta ccgtcgatac caccattcag gaacatcgcg atcgcggtga tacctataac
    42421 ggtcagttct ttaccagtgg accattaatt gatggtgtgc tgggaatgaa agcttacggc
    42481 agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga taccggagaa
    42541 acgccgcgta ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc ctggacaccg
    42601 aatcaaaatc acgattttac tgccggatac ggtttcgacc gtcaggatcg tgattccgac
    42661 tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg
    42721 gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc
    42781 aacagcagcc cgataacttc cgaaagcaat gcggtcgacg gcaaatacac gttgccgctg
    42841 acggcaatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa actgagcgat
    42901 gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt
    42961 gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac
    43021 gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc
    43081 gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa
    43141 cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg
    43201 gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa
    43261 ggctggctgg aaggggttga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt
    43321 atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt
    43381 tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa
    43441 gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
    43501 tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
    43561 tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa
    43621 gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg
    43681 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
    43741 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac
    43801 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
    43861 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
    43921 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
    43981 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact
    44041 ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
    44101 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    44161 ctaaatagct gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    44221 caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt
    44281 gaccacccat tgaatgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa
    44341 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    44401 gttgcgtggc gttaagataa aaccccgcgc cctggcctaa atcgtagcca tcatcgttgg
    44461 caacctgttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    44521 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    44581 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    44641 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    44701 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    44761 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    44821 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    44881 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    44941 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    45001 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    45061 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    45121 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    45181 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    45241 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    45301 ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat
    45361 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc
    45421 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg
    45481 ctgggcacca ataacgtggc tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt
    45541 ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc
    45601 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    45661 aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    45721 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg
    45781 gtacggtgct attacgccgc aggatgcatg gacctgggca tttcttgatc tcatcggcca
    45841 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgct ggactgcaaa tgttgctgcc
    45901 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttattgg gctttattca
    45961 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    46021 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg gcgtcatgct
    46081 ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag
    46141 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    46201 cggcgttgat gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    46261 cgcacagtat ggctggcaac tggctgggat gatgctcatt ggtgcggcgc tgatgcgcag
    46321 cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    46381 ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc
    46441 gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc
    46501 gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttaagccgct ttaagctggt
    46561 gctggcaatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    46621 ttgtaccacg cttttttacc acctcggttt gtttatgcat tttgatcgcc tggagctgct
    46681 ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta
    46741 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac
    46801 ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    46861 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    46921 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    46981 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    47041 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    47101 tcggccaaat gccaatgccc aggcgctggc aactcaggtt agcgatacca ttggcgtggt
    47161 ggtgatggac gtttctgatg cttttttcgg cgcgctggta aaagcggtgg atctggtcgc
    47221 ccagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    47281 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
    47341 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    47401 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    47461 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
    47521 ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    47581 ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga
    47641 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctgtat ttgcttataa
    47701 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    47761 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    47821 aacgaccgtg cgttatccca ttgcttcaat ggcgaaatta gccaccgaac tggccttgca
    47881 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    47941 tcgccattct gtcgcaacac gccagaatgc ggcgacgatc actaactcaa caaatcaggc
    48001 gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg
    48061 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    48121 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    48181 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccccgccga
    48241 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    48301 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    48361 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa
    48421 ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    48481 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    48541 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    48601 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    48661 cgacaaagcc tactacgttg gcactgattc caaagagtcc ggcattattc agggcgattt
    48721 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    48781 attcgtattg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    48841 gattaaagag ctgaacgaca aaggcattaa aactgaacag ttacagttag ataccgctat
    48901 gtgggatacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    48961 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact
    49021 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    49081 ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc
    49141 gaaagcgacc tttgatctgg cgaaaaactt ggccgatggc aaaggtgcgg ctgatggcac
    49201 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    49261 cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat
    49321 tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata
    49381 cttgttggaa atgagcggta tcaacaagtc ttttcctggt gttaaggcac ttgataacgt
    49441 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gcgcaggaaa
    49501 atcaacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    49561 ccagggtaaa gagatcgatt tccattccgc caaagaagca ctggaaaatg gtatttcgat
    49621 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctggg
    49681 gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    49741 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt
    49801 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    49861 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    49921 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    49981 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    50041 gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca
    50101 gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac
    50161 gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct
    50221 cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    50281 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc
    50341 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat
    50401 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    50461 taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg tgattgattc
    50521 gatgcgggta aaaacgccgg gtcatcggac gcaaattggt tcgctctccg gtggtaatca
    50581 gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa tgctcgatga
    50641 accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact
    50701 ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    50761 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac
    50821 aacaacgcaa aacgaaattc tacgtcttgc gtctttgcac ctttaagatt aggggcttcc
    50881 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    50941 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    51001 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    51061 agggttaatt gtcacccagg ggaccgatct ttctgctggt cgtcaggtag ggctggcggc
    51121 agtggtggct gcgacattat tgcagtccat ggataacgcc aacaaagtgt tcccggaaat
    51181 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    51241 tttgatcaac ggtctgatta tcgcttatct caacgtgacg ccgttcatta ccacgctcgg
    51301 cacgatgatc atcgtctatg gaatcaactc gctctattac gactttgtcg gggcgtcgcc
    51361 aatttctggt tttgacagtg gcttttctac ctttgctcag ggctttgtcg cgctggggag
    51421 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    51481 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    51541 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    51601 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    51661 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag
    51721 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    51781 ctatggcctg acgtatatcg gcgtaaaccc atactggcag tacatcatca aaggggcgat
    51841 tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaagt gatgtgattg
    51901 tggatattgc cggatgcgct gcatccggca atcggtgcca aatgccggat gcgtctctgg
    51961 ttttacaacg ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca
    52021 atacaaccca ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta
    52081 caatgcggtg tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag
    52141 gaaatataac agcgtccaca gccaacacat ttatcaagat tgatacgggg atagacaata
    52201 taactgcggt ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc
    52261 tgcagcgaat caaatccctg atcggcgaga taatgcgaca acccgcttgc catatcttcc
    52321 actatccgat acccgtactg catgatgccg gtggtcacct gtaacgttgc tgcgccgagc
    52381 aataaaaact cagccgcatc ctcccaggtt tcaatgccgc cgataccgct gattgggaaa
    52441 tcgcgcagtt ctggatgggt gcgcatctgc tgaatgaagc gcagggcgat cggttttacc
    52501 gctttaccgg aatatccgga aatactcgat tttccgttaa cgatcggcat accgattttc
    52561 tgattaagat cgatattggt gatggattta acggtgttaa tcgccgcaat gccatctgcg
    52621 ccgccgcgct tcgccgccag cgccacttcg cacatatcac cgatattcgg cgtcatcttc
    52681 gccagcattg gcagcgtgga accccgtttc actgcccgac aatatttttc taccagctcc
    52741 gggctttgcc cgacatcgct acccatcgca tgagaagtca tttgcggaca ggagaagtta
    52801 cactcgatca tatccgcgcc agcttcttgc accaggcgcg ccagctcctc ccattgctgc
    52861 tcattttccc ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagctga
    52921 cgcagggcgg ccagattctc ttccaacgga tgttcagcaa tctgctccat atttttgaag
    52981 ccgataaaac cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg
    53041 ataaaaaagc cgatcgtttt aaacaccacg ccgccccaac ctgtgtcgta ggctttggca
    53101 cacatctcat agcagttgcc tacaggcgaa gaagagaggc agaacgggtt gggaaacttc
    53161 acgccgcaaa aagtaatcga aagatctttc gttaacatga gcaagctccc tctaaatagt
    53221 gatgaatcgc ctcggcggct tctttcccgg ttttcacggc atagaccacg gttttgtcac
    53281 cctcaacaat atcgccagca gcaaagactt gcggatcgcg ggtctgataa ttttgtgttt
    53341 tgatggtgtt acgctgcggc tctaactccg caaaggcatc cagtctggca tgctgaccga
    53401 cggcgagaat aattttatct gccgccatcg tcagttcgcc cggtaaccgt acatgcttaa
    53461 acgtgacttt attgccttcc acggctactg gcgtgaatcc atcgatgatc gaaacgccca
    53521 gctcccgggc gctggcaaac tctttttcgc ttgccggaaa ctcatctaac tcttcacgcg
    53581 ctacgcaggt taccgcctga cagccgagaa ctttcagcgt gctggctacg tccatcgcaa
    53641 catcaccgcc gccgataatt aatgcgcttt gtggaatgct gatatcgcct tgcgcctgtc
    53701 gtgcacgttg caggaagtcg acggcaatct caacgtcgct atgctcaaac agcgataacc
    53761 cggaaccgct cgataacccc acggtgacca gtaccgcgcg gttttctgct ttcagttgct
    53821 ccagggtgag tgtgttaccg acttcgttgt tgcacttaat ggtcacgccc attttttcaa
    53881 tacgggcgat ctctgcatcc agcactgatt gcggtaaccg gaattgcgga ataccgttac
    53941 gcaaccagcc accggggtgc gcttctttct cataaatcgt gacgtcataa ccctggtttg
    54001 tcagtgttac actggcctgc aatccggcag gacctgcgcc aataatcgcg actttgccga
    54061 gcgttttagt accgggctga taaatttcca tcccggtttg ttgttcaaaa tcagtaacaa
    54121 aacgctgtaa gcgaccgata tcaatgggag tatcaacacc ggcacgggta caaccgcttt
    54181 gacataattt ctccgtcggg cacactctgg cacaaacggc accgagggca ttattttcgc
    54241 gaattgtctc ggcagcgcct ttaaaattac gaaagtagat tgagcgaata aatttccccg
    54301 gatcggtctg tgccgggcaa gcctgactac agggagcgtc atgacataat aaacagcgag
    54361 aggcttcttt aatcgccagt aaaggcgtaa aagccggagt gagttcatcc agataatttt
    54421 gttgcggcat gtgatcttcc ttaatgctat gagtaattaa ggcgaacgct aaatatactc
    54481 gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca cttacttatg
    54541 taagctcctg gggactcact cacttgtcgc cttcctgcaa ctcgaattat ttagagtata
    54601 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttatactgt gaaaggattc
    54661 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    54721 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    54781 cgatagataa aattccgaag ccgatatatg ccaccatata agcgaaaaag agtccgggat
    54841 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    54901 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cggaataagg aaaaaaacgg
    54961 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    55021 taactgttcg ggtgtgacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    55081 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    55141 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    55201 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgcccgctc
    55261 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    55321 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    55381 accagcagcg attaaatctt tgcgcatagt catcggctca ttataacttt gcaatgcgtt
    55441 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    55501 aatgcggtag cgataatact gattaattac gccagtacga taatattttg ctgttccgag
    55561 caccacaccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    55621 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    55681 taacaggctg aggaacacgc gctttaacat gcaggagact cgatctaagg aaaatttcca
    55741 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    55801 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    55861 taagcgagaa gcactcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    55921 gcatcccact gaatcaacgg cgcatcggcg ttttctgcca gaaccgcgcg ctggatatcc
    55981 gggtaatcat aacccttgag attcaataga attaacgctc cctgcaacgg tggcagagtc
    56041 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    56101 agcgcgacac cgcttggcga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    56161 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    56221 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    56281 tgtgcctcgc ggcccggcag atgaatacgc agatccagac cgctgttcag ttcattcata
    56341 aactgacggc agtgaccaca aggcgtgtag ttaacggtga tggctgcaag cgctttttca
    56401 ccactcaacc aggcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc
    56461 gcaccaataa attccatatt ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt
    56521 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    56581 gcgaatgcca gcgcgtcttc gtccagcccc gttgcgctct tcagcgatga gacttgctcc
    56641 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    56701 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    56761 catgtaatgg gataacagtt tacgaacccg ttaaggcttt atatgtgatc tgaatctcat
    56821 taattatgca acgcgtgaaa cttacatcaa aaatgcgcga cgcatcgcaa attttagccc
    56881 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    56941 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    57001 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    57061 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    57121 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    57181 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    57241 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    57301 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    57361 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtagct gtcgtaagag
    57421 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    57481 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    57541 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    57601 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    57661 ttgcacagga aaccactacc gggccaaact gtgcgcggag caaatcaaaa tattgcatga
    57721 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    57781 gattgaccca ttttgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    57841 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    57901 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    57961 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    58021 ccgatcacat taagaaatca gcgcttattt tttcgctccc cggcattacg caatgcgcaa
    58081 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgt
    58141 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattatttt gcgggtgatg
    58201 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    58261 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    58321 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    58381 catccggtac gctggtgcgc gttcagttgt taggtcagtt cccacaatgg ctggcagaga
    58441 acgccgcccg tgcggtggaa ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt
    58501 cgaaaacggt taacggcagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    58561 accagggtgc aaaagcgatg cgtgaagctg taccagcgca tttgcccgtc agcgtaaaag
    58621 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    58681 ctggcgctac ggagctggtg gtgcatgggc ggacaaaaga gcagggttac cgcgcggagc
    58741 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    58801 acggtgaaat ctgggactgg cagagtgcgc aacaatgcat ggcgatcagc ggctgcgacg
    58861 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggta gtaaaatata
    58921 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    58981 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    59041 tgcgtaaaga atacgatgaa gcaacgggat tatttcagca tgttcgggtg ttgaataatt
    59101 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaaccatag
    59161 tgatgttttt tgacaatgtg ttagctcttt atcaataggc cgcacggttg tttggtggcg
    59221 tcaaaattgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg
    59281 cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagccgagg
    59341 gcaccgcgca gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgatt
    59401 ggccgccccc tgcgcgctcc ttgcgccagt gattatatga agcaatgcac atgacaggag
    59461 cgcaacagga tgagagactg aaatattctc gttattttcg gacatgatga cattaattaa
    59521 ttttaaaata tcatcttaaa tacgccagtc accaccagta gcccaatcag gaaaataatc
    59581 aatacagccc agagtataat tttcattggc gttcctttat ttgtgagtgc tgaataagta
    59641 taggaaaaaa cgaagccctt tgccgttatc cataaacgat taagataaat cggaagatat
    59701 cattcactta gtgtatattt ttctggatat gtcgttttat tgctactgat gtattgccac
    59761 gcgaaactaa aaaatatatc gcaacacacg gtaatctccg cgcacctttt cttacctgat
    59821 tattccccca tgaatcgtga ttccttttat cccgccatcg cctgttttcc gctgttactg
    59881 atgctggccg ggtgtgcgcc tatgcatgaa acccgccagg cattaagcca gcaaacgccc
    59941 gctgcacaag ttgacaccgc attacccacg gcgctgaaaa atggttggcc agacagccaa
    60001 tggtggctgg agtatcacga taatcaactc acttccttaa ttaacaatgc actacagaac
    60061 gcaccggata tgcaggtggc tgaacagcgc attcagctag ctgaagccca ggcgaaggca
    60121 attgctactc aggatggtcc gcagatcgac ttttctgcgg atatggagcg gcaaaaaatg
    60181 tcggcagaag gcttaatggg gccgtttgct ctgaacgatc cggccgcagg tacgaccggt
    60241 ccgtggtaca ccaacggaac ttttggctta acggcgggct ggcatctcga tatctgggga
    60301 aagaatcggg cggaggttac tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc
    60361 gagcaaaccc gccaattgct ggctggcagc gtagcccgcc tgtactggga gtggcaaacc
    60421 caggcggcgt taaacacggt cttgcagcaa atagaaaaag agcagaacac cattatcgcg
    60481 accgatcgcc agctatatca gaacgggatt acttcttcag ttgaaggtgt ggaaaccgat
    60541 attaatgcca gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat gaaaattatt
    60601 gaggcacggt taagcgcact tacaaataac cagacaaagt cattgaagct taaaccggtc
    60661 gcgttgccga aagtggcaag ccagcttcct gatgaactgg ggtactcctt actggcccgg
    60721 cgggcagatt tgcaggcggc gcactggtac gttgagtcat cgctaagcac cattgatgcg
    60781 gcaaaagcgg cattttatcc tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg
    60841 cacttaagcg atctgttccg tcattccgcg cagcaaatgg gcgttacggc aggcctgacg
    60901 ctacccattt tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc
    60961 aacttgtcta tcgccagcta caacaaagcg gtggttgaag cggtgaatga cgtggcgcgg
    61021 gcagccagtc aggttcagac acttgcggag aaaaaccagc atcaggcgca aattgagcgc
    61081 gatgccttgc gtgtggtagg tcttgcgcag gcgcgcttta acgcgggcat cattgctggt
    61141 tcccgcgtca gcgaagccag aatccccgcg ctgcgtgagc gggccaatgg cctgttattg
    61201 caagggcagt ggctggatgc ttccattcaa ctcactggtg cgttgggcgg gggttacaaa
    61261 cgctgatgat atgcgccttc tatacttaac gtttattcag cgttaagtgg agaactcgat
    61321 ggcacaggtt gcgattatta ccgcctccga ttcggggatc ggcaaagagt gcgcgttatt
    61381 actggcgcag caggggtttg atattggcat tacctggcac tcagatgaag aaggggcaaa
    61441 agataccgcg cgtgaggtag ttagccacgg cgtacgtgcg gagattgtgc agctggatct
    61501 cggtaaactg ccagaaggtg cacaggcgct ggagaaactc attcaacggc tggggcgtat
    61561 tgatgtgctg gtgaataatg cgggcgcaat gaccaaagcg ccgtttcttg atatggcttt
    61621 cgatgagtgg cgcaagattt ttaccgttga tgtcgatggt gcatttttat gctcacaaat
    61681 tgcggcccgc cagatggtga aacaagggca gggtggtcgc atcatcaaca ttacgtcggt
    61741 acatgaacat acgccgctgc cggatgccag cgcctacaca gccgctaaac atgcgctcgg
    61801 cgggttaacc aaagcaatgg cgctggagtt ggtcaggcat aagattttgg tgaacgcagt
    61861 cgcacctggg gcgatcgcta cgccaatgaa cggcatggat gatagcgacg tgaagcccga
    61921 cgcggagcct tcgattcctc tgcggcgttt tggcgcaacg tatgagattg ccagcctggt
    61981 ggcgtggctt tgttcggagg gcgcaaatta caccaccggg cagtcgttga tagtggatgg
    62041 cggctttatg ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg tgtgtggata
    62101 tttcaatggg taaaattttg gtaggcatga taagacgcac aagcgccgca tcaggcatgg
    62161 tgcaccggat gtcggatgcg gcgcgaacgc cttatccagg gcaatggttt tacgcctgat
    62221 tatccggctt tttcttcccg cgtcgtttca gccaccagcg cacgcctacc accagaacca
    62281 cgaccagaat caaccagacc cagtgcttca aatgctgatc gagattgtgc aaccacggcg
    62341 caatcacctg accaccagcg taaccgatag tggtaaaaat caacgcccag gcaaatgcgc
    62401 cgagaatatt aagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg
    62461 ggccaatcac ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc
    62521 gttggataag tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca
    62581 gcttgccgcc aaaccgccgc ccgcacagat aaagcacctg gtcgccaatc atgccgccaa
    62641 gcgccacaga aagtaccacc agcgggaact ttaatagccc ctgatgcgcc gcaacgcctc
    62701 ccagcaaagt cacggtttca ccttccgcca ggctaccgat caccagcgcg gcataaccat
    62761 attgtgagat gagtgtattg agatccatag gccagtctgt ctccctttgg ttgcctgtaa
    62821 gcatacaccc tggaaatcat tcacgctgaa aaaggacgta catcagacgg tgcaaattac
    62881 atgagcgaac aaaaaataat cacaagctga cttatacttg aactgtcagg ttcatgaact
    62941 cacaacaagg gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa
    63001 tgcaggtgat taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc
    63061 tgatggcggc gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt
    63121 ttggcgatgg cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcctgt
    63181 tttatggcgt tatgctggct ggggttgcgg taatggggcg ggtcatctgg tggatggcgc
    63241 gtaattatcc gcagcgtccg tcacttgcgc actgcatggt cttcgcgggc tacgttgcaa
    63301 ctccgctgtt tttaagcggt ctggtggcgc tttatccgct ggtctggttg tgtgcgctgg
    63361 tcggcacggt ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc
    63421 tgaatatcaa caaagaggaa ggccttagct tctctagttc gacgctggcg attggcgtgc
    63481 tggtactgga agtgctgctc gccctaaccg ttatactatg gggctacggt tatcgtttgt
    63541 tctagaaagt tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttcctc
    63601 acgattctcc tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta
    63661 aatccttact ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat
    63721 gccgaaattt cgagtttctt tatttagcct ggccctgatg ctggctgtgc ctttggcacc
    63781 gcaggccgtt gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg
    63841 tagcgcgatg attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct
    63901 ggtgcgtccg attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg
    63961 actgccgctg gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt
    64021 ctattcgcgc gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct
    64081 gatgtcttca gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa
    64141 agcctttatt aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt
    64201 tgttgaacct accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact
    64261 gctcattgcc agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat
    64321 ggcaaccttc tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta
    64381 tcgcgataac tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg
    64441 tctggtgatg cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt
    64501 tggcaaatat acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa
    64561 agtgatgcct gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc
    64621 ggcggggcag acggcacaga acgattaagt gaattcggat ggcgatactc tgccatccgt
    64681 aatttttact ccacttcctg ccagtttttc cgtttactgg ttttaccgat ccccggattc
    64741 atgctgttgg tcggatcgtt ctcgcgatag aacttctgca acgtctccgg tgctttatac
    64801 aaatgaccga cgttatgctc ggcagggtac tgcgcgccgc gctgctgtag cagttccagc
    64861 atctgttctt ttaacgcatg cacatccacg ccttttttca ctatgtaatc ctgatggaag
    64921 acatagcaca taaaatggcc gtaatagagc ttgtgcacca gctggctgtc gatctccggc
    64981 ggtaaatgct cataccactc ggtgtcgtta cgccgcagag cgatatccaa cgccagaatg
    65041 tcttcgactt catcggaatg caccgcctga taacgaattg ctgcgcccgc agcggcgaaa
    65101 cggtgtaaaa acgctttgct gccttcctcc ggcgtacaga caaagaaatc gccttcggcc
    65161 tgtttgaaat aatctaccag ccacgatttg gcttcaccga cgccatcgcc cgccattttt
    65221 aacagcagat gatgctcgta tttatcgcgc cagtttttca tgcgcggcgg taaatggctg
    65281 gggaacaggt gaccgaattt ttgcatcgca cggtcagtaa aatgcggacg gaagaatttc
    65341 actttctcca gcatcgcatc ggtgcgtccc ttgagattaa agaagaacgg catcttgtcg
    65401 gtgcctaact tatcaatcat caggaaggtg tctttgccgt atttttccgc aatatcgtag
    65461 atatcccggt gcatatattc cccggcaacc ggcagatttt cgaagttagc cagaatatga
    65521 cggcggattt cggtcagcac ttccggctgg ttggtgccaa tataaaacac ctgctgattt
    65581 ttttccgcct cgaaggtatc aagacgtact gcaaagacgg ccagcttccc ggcgcaacca
    65641 gaagattcaa ataaccgatc cggatcggcg ttataacgtg cgggcgtgtc ggcttcaata
    65701 tcacgaacgc ggtggacata gtcataatcg tgggcgtgac gaccatcgtg acgcacatcg
    65761 tcatctttga tgcgatcatc atccagcttg ctaaggatct gctccggcgt ttcgcccaga
    65821 tcaatcccca gatggttcac cagcgtcagt ttgccgtctt catttatacg cgcgaataac
    65881 gacatttcgg tatacgccgg gccacgttgc accagcgagc cgccggagtt gttacaaata
    65941 ccaccgatga ccgatgcgcc tatacacgat gatccaatca ctgagtgcgg ttcgcgtccc
    66001 agcggtttga gggctttttc cagcgaatag agcgtggtgc ccggataggc cagcacctgt
    66061 tcgcccttgc cgagaacgtg cagcttgtcg agacgcaggg tgctgatgat cacgatatcg
    66121 cgatcatagt cgttaccgtt tggcgtcgat ccttcggtca ggcctgtatt ggcagcctgc
    66181 atcagaataa ttttgtcggc ggtgacgcag gctttcagca cccgccacaa ttccagtagt
    66241 gagccaggga aaacgacagc cagcgcgtcg ccctgaccag aacggaagcc cttgcgatag
    66301 cgggccgttt ttgcgggatc ggtgagcagg tgtgaatgac cgaccagacg ggcaagttca
    66361 ttcaaaaagg ctttattatc agttgttgtc atggaagaca ttttccactc cttgtggtgg
    66421 cgaaaaaata tcacaaacta gaatagcgcc ttgtatgaca attcagcgag tgaatatttt
    66481 gaaaattcag acaataaact gagcattctt tttctctctc catcatgctt atggcaaact
    66541 acggtttttg caccattttc gctgcctcgt cgggcaacag agaaagaaaa atccatatga
    66601 aatggctatg ttcagtagga atcgcggtga gtctggccct gcagccagca ctggcggatg
    66661 atttattcgg caaccatcca ttaacgcccg aagcgcggga tgcgttcgtc accgaactgc
    66721 ttaagaaaat gacagttgat gagaaaattg gtcagctgcg cttaatcagc gtcggcccgg
    66781 ataacccgaa agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca
    66841 acaccgtaac ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc
    66901 tgaaaattcc tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga
    66961 ttagcctcgg tctggcctcg tcttttaacc tcgatgcagt gaaaacggtc ggacgtgtct
    67021 ctgcttatga agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct
    67081 cgcgcgatcc gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct
    67141 caacaatggg taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgctact
    67201 cggtgatgac cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt
    67261 acaacaccgt cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag
    67321 cggggctgga cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc
    67381 cagccacctc cgattcttgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag
    67441 gcatcaccgt ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag
    67501 acccggaaga tgcggtgcgc gtggcgctga aatccggaat caacatgagt atgagcgacg
    67561 agtattactc gaagtatctg cctgggttga tcaaatccgg caaagtgacg atggaagagc
    67621 tggacgatgc tgcccgccat gtactgaacg ttaaatatga tatgggattg tttaacgacc
    67681 catacagcca tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc
    67741 tgcaccgtaa agaagcgcgt gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc
    67801 tcgaaacgtt accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca
    67861 gtaaacgtga cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg
    67921 tactgaccgg gatcaaaaat gccgtcggtg aaaacggtaa agtgctgtat gccaaagggg
    67981 cgaacgttac cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca
    68041 aagtcgatcc gcgctcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat
    68101 ctgatgtggt ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc
    68161 ggaccgatat cactattccg caaagccaac gtgacttgat tgcggcgctg aaagccaccg
    68221 gtaaaccgct ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc
    68281 agcaggctga tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg
    68341 ccgatgtatt gtttggcgat tacaacccgt ccggcaagct gccaatgtcc ttcccgcgtt
    68401 ctgtcgggca gatcccggtg tactacagcc atctgaatac cggtcgcccg tataatgccg
    68461 acaagccgaa caaatacact tcgcgttatt ttgatgaagc taacggggcg ttgtatccgt
    68521 tcggctatgg gctgagctac accactttca ccgtctctga tgtgaaactt tctgcgccga
    68581 ccatgaagcg tgacggcaaa gtgactgcca gcgtgcaggt gacgaacacc ggtaagcgcg
    68641 agggggccac ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg
    68701 tgaaacagct gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca
    68761 gcttcccgat cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg
    68821 agcctggcaa gttcaatgta tttatcggca ctgattccgc acgcgttaag aaaggcgagt
    68881 ttgagttgct gtaatgcttt agtaagggcg cgacgtttgc gccctttgta ggccggataa
    68941 ggcgctcgcg ccgcatccgg caggaaacgc cggaccgaat atgcctctta agtatttatc
    69001 tgattctgca actgaatcct tccgctcaag ctaaccccgc cattatcaac tatgcttttc
    69061 tcttaattcg ctgaaaacaa cagtaaaaga atgaggaaag catcatgcca ctcttaaagc
    69121 tctgggcagg ttcactggtt atgttggcag ccgtgagcct gccgctacag gcggcttccc
    69181 ccgttaaagt cggttcaaaa atcgataccg aaggtgcgct actcggcaat atcattttgc
    69241 aggtgctgga aagccacggc gtaccgacgg tgaataaagt gcaactcgga acgactcctg
    69301 tggtgcgcgg ggcgattact tccggtgaac tggatatcta tccggaatat accggcaatg
    69361 gcgctttctt ctttaaagat gaaaacgatg cagcgtggaa aaacgcgcag caaggttacg
    69421 agaaagtcaa aaagctcgat tcggagcaca acaagttaat ctggctgacg cccgcgcctg
    69481 caaataacac ctggactatc gccgtgcgtc aggatgtggc agagaaaaat aaactcactt
    69541 cgcttgccga cctgagtcgt tatctgcaag agggcggcac cttcaaactg gcagcctcgg
    69601 cagagtttat cgaacgcgcc gatgcgttac ccgcgtttga aaaagcctat ggctttaagc
    69661 tcggtcagga tcagttgctg tcactggctg gtggcgacac ggcggtgacg atcaaagccg
    69721 ctgcccagca aacctctggc gttaatgctg caatggctta cggcactgac ggcccggtag
    69781 cggcgctggg gctgcaaacc ttaagcgatc cgcaaggcgt gcaacctatc tacgcgcctg
    69841 caccagtggt gcgtgagtcg gtgctgaaag agtatccgca aatggcacag tggctacagc
    69901 cagtcttcgc cagcctcgat gcaaaaacat tgcagcaact gaatgccagc attgcagtgg
    69961 aaggactgga tgccaaaaaa gtggctgccg actacctgaa acaaaaaggg tggacgaagt
    70021 aatttcccgt gacttatcta cgtattaatc ctgttctggc gctgctgctg ttgctgacgg
    70081 caatcgcagc agcgctgccg tttatcagct acgcgcctaa tcgtttagtc tcgggtgaag
    70141 ggcgacatct ctggcagctg tggccgcaaa cgatctggat gctggtgggc tttggttgcg
    70201 cctggctgac agcctgtttt attcccgcta aaaaaggcag catttttgca ctcattctgg
    70261 cgcaattcgt cttcgtattg ctggtgtggg gagctggaaa agcggcgacc caactggcgc
    70321 aaaatggcag tgcgctggcg cgtaccagcc tcggcagtgg tttctggctg gctgcggcgc
    70381 tggcattgct ggcctgtagc gatgccatcc gccgaatctc cacgcatccg ctgtggcgct
    70441 ggttgttgca tatgcagatt gccattattc cgctgtggtt gctgtactcc ggcacgctta
    70501 acgatctctc gctaatgaaa gaatacgcca accgtcagga tgtgtttgac gacgcgctgg
    70561 cacaacatct gacgttgctg tttggtgcgg tgctgcctgc gttagtgatt ggtgtgccgt
    70621 tgggcatctg gtgctacttt tccactgcgc ggcagggggc aattttttct ctgctcaatg
    70681 tcattcagac cgtgccttcg gtggcgctct ttggcctgtt gattgcgccg cttgccgcgc
    70741 tggtgacggc ctttccgtgg ctggggaagc tcggcatagc aggaaccgga atgacacccg
    70801 cactgattgc gctggtgctc tatgccttgc tgccgctggt gcgcggcgtg gtagtaggct
    70861 tgaaccagat cccgcgcgat gtgctggaga gcgccagagc gatggggatg agcggggcgc
    70921 agcgattcct gcatgttcag ttaccgctgg cgttaccggt atttctgcgc agcctgcggg
    70981 tggtgatggt gcaaactgta ggtatggcgg tgattgcggc gttaatcggc gcaggcggtt
    71041 ttggtgcgct ggttttccag gggctgctaa gcagcgccat tgatttagtg ttgctggggg
    71101 tgatcccggt aattgttctg gcggtgctga tcgatgccct gttcgatttg cttatcgcac
    71161 tgctgaaggt gaaacgtaat gattgaattt agccatgtca gcaaactgtt tggcgcacaa
    71221 aaagccgtta acgatcttaa tctcaatttt caggaaggga gtttttcggt gctgattggc
    71281 acatctggct ccggcaaatc caccaccttg aaaatgatta accgcctggt ggaacatgac
    71341 agcggagaga tccgctttgc cggagaagaa attcgctcgc tgccagtact ggagttgcgc
    71401 cgccggatgg gctatgccat tcaatctatt ggcctgttcc cccactggag cgtggcgcaa
    71461 aacatcgcta ccgtgccgca attacaaaaa tggtcgcggg cacggattga cgatcgtatc
    71521 gacgaattaa tgacgctact ggggctggag tcaaatttgc gtgagcgtta tccgcatcag
    71581 ctttccggtg gtcagcagca acgtgtggga gtggcgcgcg cactggctgc cgatccgcaa
    71641 gtcttactga tggatgaacc ttttggcgca ctggacccgg taacgcgcgg cgcgttgcaa
    71701 caagagatga cgcgcattca ccgtttgctg gggcgcacta ttgtgctggt cactcatgat
    71761 attgatgagg cgctaaggct ggcagaacat ctggtattga tggatcacgg tgaagtggtg
    71821 cagcagggga atccgctgac gatgctgact cgtccggcga atgattttgt ccgccagttt
    71881 tttggacgta gtgaactggg ggtgcgcctg ctttcgttac gtagtgtggc ggattacgtg
    71941 cgtcgcgaag aacgggcaga aggtgaggca ctggcagaag agatgacgct acgcgatgcg
    72001 ctctccctgt ttgtcgcgcg gggatgcgag gtgctgccgg tgatgaacac gcagggccag
    72061 ccttgcggca cgctgcattt tcaggatctg ctggaggagg cgtaagcgta tgaaaatgtt
    72121 gcgcgatccg ctgttctggc tcattgcttt gtttgtggca ctgattttct ggctgcctta
    72181 cagccagccg ctgtttgctg ccttgttccc acaactgcca cgacccgttt atcagcaaga
    72241 aagttttgca gctctggcac tggctcattt ctggctggtg ggaatttcga gtttgtttgc
    72301 ggtgatcatt ggcactggtg ccggaattgc tgtcactcgc ccgtggggcg cggaatttcg
    72361 cccactggtg gaaactattg ccgccgttgg acagactttt ccgcctgtcg cagtgctggc
    72421 gattgccgtt ccggtgatcg gctttggtct gcaaccagcg attatcgcct tgatccttta
    72481 cggtgtgctg cccgtcctgc aggcgacact tgccgggctg ggagcgattg atgccagcgt
    72541 gacagaagtt gcgaaaggta tgggaatgag tcgtggtcag cgactgcgta aggtcgagct
    72601 accgctggcg gctccggtga ttctggcggg cgtgcgaact tcggtgatta tcaacattgg
    72661 tacggcgacg atcgcctcaa cggtaggggc cagcacgctg ggtacgccca tcatcatcgg
    72721 gcttagcgga tttaataccg cgtatgtgat ccagggggcg ttactggtgg cactggcggc
    72781 gatcatcgca gaccgcctgt ttgaaaggct ggtgcaggcg cttagccagc acgcaaaata
    72841 aaggtataac ctgcgagcat gacgccacca attccgccta acgccataaa caggaacagg
    72901 gcgatgaccc caattttagc tatgcgcata ttgcactcct tatgttaacg aaaggattgt
    72961 acagtaaagc gcatttgtta acgaatcatt aaatgccgag tgggaaaata tcatggcctt
    73021 gttcttgcca actggtgagt tgctgctgtt gggcggaggt tcgattttca ccgcaccaca
    73081 ccagcaatgt acggccttcg aatagttcag ggcgtagttg attgagcgag tgggcgagga
    73141 catcaatgcg ccatccttgt tgactggcaa tccagccctc cagccacaga cgggtggtat
    73201 cctgaatatt ccagccaacc accagcgcat ctttaccctg ttttttacgt gccgaagcca
    73261 gacaaatggc gatgtagttg atcagtacgc cgtcgaggat cgccagcagc gcctggagag
    73321 tcggttgttg gcactgaagc cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct
    73381 gggcggggta atcctgaccg cgctctttga tccacgttcg caggctatgc agattgccgc
    73441 tttgcaggta ggtcagtaat gtttcttgct gatcgcgcca gccgttctgc acatcaacat
    73501 tttcattact gagcagcatt ttaactttgc tgacctgcac gccgttgtcg atccagcgtt
    73561 tgatctcgcg gatacggtca atatcggcat cgttgaacag tcgatgaccg ccgtctgtcc
    73621 gttgcggttt cagcaacccg taacgcctct gccacgcgcg taacgtgaca gggttaatat
    73681 cacaaagcaa cgccacttca ccaattgtgt aaagcgccat cgtctcaccc ttgctcgcga
    73741 ggtcccggtt taactttaga cgcagttttg cgaaccaggt agttttgccc gttttttgtg
    73801 catctatagg gtgattttat ttttgccagg cgattttgag tgatcgtact cacgaattct
    73861 catttttctg caagagttca aagaaagtta aacgcaggca atgtatgtta cgcgttttaa
    73921 agggaagtgt ggtttgcggg tatgtacgat tttaatctgg tgttgctgct gcttcagcag
    73981 atgtgcgttt ttttagtcat tgcgtggtta atgagtaaaa cgccattatt cataccgtta
    74041 atgcaggtca cggttcgtct gccgcataaa tttctctgct acatcgtctt ttccatcttc
    74101 tgcatcatgg gcacctggtt tgggttgcac attgacgatt ctattgccaa tacccgtgcg
    74161 ataggcgcgg taatgggcgg cttactcggc ggtccggtcg tcggtgggct ggttggtctg
    74221 accggcggct tacatcgata ttcgatgggg ggcatgaccg cgctaagttg catgatctca
    74281 accatcgttg aaggattgct cggcggcctg gtacacagca tcctgatccg tcgcgggcgc
    74341 actgataaag tctttaaccc cattaccgcc ggtgccgtca cgttcgtcgc tgaaatggtg
    74401 caaatgctga tcatccttgc gatcgcccga ccttatgaag atgcggtgcg tctggtgagt
    74461 aatattgctg caccaatgat ggtcaccaat accgtcggcg cggcgctgtt tatgcgtata
    74521 ttgctcgata aacgcgcgat gtttgaaaaa tacacttctg ctttttctgc cactgcgctg
    74581 aaagtggcag cctcgacgga aggcattttg cgacaggggt ttaacgaagt gaacagcatg
    74641 aaagtggctc aggtgctgta tcaggagctg gatattggtg cagtcgcgat taccgatcga
    74701 gagaaattgc tggcctttac cggaattggt gacgaccacc atttacccgg caaaccgatt
    74761 tcttcgactt acaccttaaa agcgattgaa accggtgaag tggtctacgc tgatggcaac
    74821 gaagtacctt atcgttgctc tttgcatccg caatgcaaac tggggtcgac gctggtaatt
    74881 ccgttgcgtg gcgaaaatca gcgcgtgatg ggcaccatca aattgtatga agccaaaaac
    74941 cgtttattca gttcaataaa ccgcacgctg ggcgagggga ttgcgcaact gctttcggcg
    75001 cagattcttg ccgggcaata tgagcggcaa aaagcgatgc tcacccagtc agaaatcaaa
    75061 ctgcttcacg cccaggtgaa tcctcatttt ttgtttaatg cgcttaacac cattaaagcg
    75121 gtgatccgcc gcgacagcga acaggccagc cagctggtgc agtatctttc cacttttttc
    75181 cgcaaaaact taaagcggcc ttcggagttt gttactctcg ccgacgaaat tgaacatgtg
    75241 aacgcttatc tgcaaattga aaaggcgcgc ttccagtcgc ggttgcaggt caacattgct
    75301 attccgcaag aattatccca gcagcaattg cccgcgttta ccctgcaacc gatagtggaa
    75361 aacgccatta aacatgggac atcacaactg ctggatacag ggcgagtggc aatcagcgcc
    75421 cgacgtgagg ggcaacattt gatgctggag atcgaagaca atgccggttt gtatcaaccg
    75481 gtaaccaatg ccagtgggct ggggatgaat ctggtggata agcgtttacg tgaacggttt
    75541 ggcgatgact atgggataag cgtcgcctgt gagcctgata gttacacccg aataacgtta
    75601 cgactaccat ggagggacga ggcatgatta aagtcttaat tgtcgatgat gaaccgttag
    75661 cacgggagaa cctgcgcgta tttttgcagg agcagagcga tattgaaatc gttggagagt
    75721 gttcaaacgc cgtggaaggg atcggcgcgg tgcataaact gcgcccggat gtgctgtttc
    75781 tcgatatcca gatgccgcgc atcagtggtc tggaaatggt ggggatgctc gatccggaac
    75841 atcgtccgta tattgttttt ctcaccgcgt ttgacgaata cgctattaaa gcctttgaag
    75901 aacatgcttt tgattatctg ctgaagccaa ttgatgaagc gcgactggag aaaacgctgg
    75961 cgcgattgcg tcaggagcgc agcaagcagg atgtttcgct gttaccggaa aatcaacagg
    76021 cgctgaaatt tatcccttgt acggggcata gtcggattta tttgctgcaa atgaaagatg
    76081 tggcatttgt cagcagtcgg atgagcggtg tctacgttac cagccacgaa gggaaagagg
    76141 gctttaccga attgacatta cgtaccctgg aaagtcgtac accactactg cgctgccatc
    76201 gtcagtatct ggttaatctc gcgcatttac aggagattcg tctggaagat aacggccagg
    76261 ccgagttgat tttgcgtaat ggcttaaccg tgccggtcag ccgccgttat ctgaaaagct
    76321 taaaagaggc gattggcctg taaaagactg ctaaaatggc tttttgcctc atcaacacct
    76381 gaaggcctca tgctaagtaa cgatattctg cgcagcgtgc gctacatttt gaaagccaat
    76441 aataatgacc tggtgcgtat tctggcgctg ggtaatgtcg aagccaccgc ggaacagatc
    76501 gccgtctggc tacgtaaaga agacgaagag ggttttcagc gttgtccgga cattgttttg
    76561 tcgtcattcc tcaacggcct gatttatgaa aaacgcggca aggatgagtc tgctccggca
    76621 ctggagccgg aacgtcgcat taataacaac atcgtgctga aaaaattacg catcgcgttt
    76681 tcgctgaaaa ccgatgacat tctggctatc ctcaccgaac agcagttccg cgtttcgatg
    76741 ccggaaatta cagcgatgat gcgcgcaccg gatcataaaa acttccgcga atgcggcgat
    76801 caatttttac gttattttct gcgtggactg gcagcgcgcc agcatgtgaa gaaaagctaa
    76861 gacgggtatg gcggccatgc gaaacatggc cgccgacaga ttatttcact tctttaaaac
    76921 cagcggcttt catcaccagt tccatttgcg ccatagtgat accttttttg gcatcttcag
    76981 cagaaacgtt gattcctgaa ataccctgca gggctttaaa atccactttt tccatatcga
    77041 tagtcacgtt ttcctgcgcg taggtatctg tataggttaa tttttcttca acacccgcga
    77101 tgtttttgta tttggcgctt aacggctcaa gtgtcttggc agcgtcttct ttggtggttg
    77161 caccaataga agcaaattga attttggttt cagaagattg cttaagcacc ttgtcacctt
    77221 tgtagacata ggtaatggca atttcagtgc cgttcagatt ggcgctgaat ttcttcgatt
    77281 cttctttgtc accgcagcca gcaagagaga aaaccagaac agatgcaaca acgagggaaa
    77341 acagcttatt gaaagccttc atgtaaaact ccattttatt taatcaagaa actggtgact
    77401 ctcaccaggg gctatataga atatgcctaa taccgtgacg tgagcagtcc ggaactggag
    77461 tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg gaataattgt
    77521 tcgcaaagta aaacaccgtt attcattgct tctacccgtg cctcgctttc tgtattacga
    77581 aattgtccca acacatgtgc cagccgataa aaaccgaccg cggagaggtc attcgccagc
    77641 aactctgcct gactaatagc gctctgttcc tgatagcgcc agccgttatg gagcagttga
    77701 ataagtaacg cctggcagcg catcagcaac tgatgagcgg tagacggcac aggcaaaacg
    77761 ctggcagaag gtagcggtgc cacaggcgca gtttccgcgt ccagcgccca ggcgcgggtt
    77821 tttgtcatca tcacccgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
    77881 gaaaccagac tcacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaacggca
    77941 aaaggagaac aggcagatgc cggaagggat aacgtcagga gattatcttc accttcgccg
    78001 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
    78061 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
    78121 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgctcaac
    78181 atttgccagg catcaggcga cagcggcaca acgctgctta agcggttgcg ggtagctaac
    78241 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
    78301 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagttttc ctgttcactt
    78361 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
    78421 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
    78481 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
    78541 ggaacttcac caacactgtc gcgccgggcc atctcttgtg cgcaatttaa tcgactgttt
    78601 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg agtgtggaag
    78661 gcatcaacgc ttgcccgtag ctgtcgtagt gattcactca cccagcgcca gttgcaggcc
    78721 tctgccgcct gcaatgcgcg gttgaatgct gcctcgtagt gaataagcgg ctggctgatg
    78781 ccgccaagcc ataatgcctg gcttaattgc tgaacatatt gacgacacgt ttggccttct
    78841 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
    78901 tggttaaatt ctgcttgctg cgctttggcc tcgacgaagg cctgcacagc cagtacgaca
    78961 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctacgggaa
    79021 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcgcccgg cgcacagaac
    79081 aactcaatgg tgatgccttt agcgaccagc gcctgcgcgc gtttgcgggt ggcatcgggc
    79141 agggtagcca gttcttccag ccagattgcc ggatcccact cttcttcttt ttccgtgggc
    79201 tggacggtgg cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
    79261 ccgctggccc cgcagctgca ctgagcctct ttcagtgcct ggctgttcgc cagctgggta
    79321 cggacaccgt cactgaaggt ggcgattaaa gcgccgttct catggctgat ttccgggacg
    79381 ttgccatttt ccagttcctt aagactgcgc ttaacaaaac cggcattgct taacgccgtc
    79441 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
    79501 ccgcaagcca tgatgccagc tcgcccggcg tcatggcggc tatttgtgcg ccgacattaa
    79561 ccagcgcctg ggccgtatcg cggtcatagc aaggcgttgc ggtgctatcg agcgctgcca
    79621 gtcccagcac tttgatgccg ctctggacac actttttcac ctgatgcgtc agcaatgatg
    79681 atgaaccccc ttcgtaaaaa tcgctcacga ggataatgac gctttttgct ggttgttcaa
    79741 taagttgccg accatactcc acggcactgg cgatattggt cccgccgccc aactgtactt
    79801 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaaccacg cttgtgtcaa
    79861 acgccaccag atgggtacga atgccgggta actgccacaa acaggccgcc atcaccgcag
    79921 agtggatcac cgagtccacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
    79981 gttcgctttg gcgtttaatg cggctgttaa agcgggggga ttcgatatac aacttgccgt
    80041 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
    80101 ccagtggaat taatgagcgg cgacggcgat cgcggacacc agaaaaagcc tgacgaactt
    80161 cctttgccag tcgcgccata atttcttcaa caacctggcg cactatctgg cgggcggcag
    80221 ccagtacttc gggattcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttccg
    80281 aaggctgcat acgttccagc acgtcgagat tcgtcaccac atcttcaatg ccgtagcgca
    80341 gtacggcatc gctttccagt cgctcaatca cctgttgcgg aaacagagtg tgaatactgt
    80401 tgatccactc aggggtggtg agatttgagc cacctaatcc accagagcgt tcaccacgct
    80461 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtggtctatc tgccgggcgt
    80521 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
    80581 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagttttgc
    80641 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcctg tggtggacaa
    80701 tgcaacggca tttgcagcgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
    80761 tgatgcgcca gcgtcccgcg ttctcgtggt ggtagccagg ccatcgccgc gcgtaaatcg
    80821 ggcagggcat tgataaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
    80881 ataaatgtcg gctgacaggc cagttgatgg cgggccagcg ccagcaaacc gtgcagcgct
    80941 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactggcg
    81001 ttaggatgtt ccagacgcat cagtgcgcca tgagccgcgc cgcgatccag cgccggggca
    81061 tgaattgcct gactgcgtcg ctccagtaag gctaccgccg cagaaagaga aatgccgggg
    81121 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcagg
    81181 tgagcgtgaa actccttttc atctggtctg ccgttagatt cacatagcca cagcgtgcga
    81241 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
    81301 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
    81361 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
    81421 gctaacgccg cctggctaag acatgtggtg atactgccga ttccacccgc gtccagcata
    81481 tcggcttcta atttatggcg tgcggcttcc tggagtgttg caccaaagca ggcggcctca
    81541 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
    81601 gcaagtgtca gtgtgcttcc ctgctggcgc acaatccctg ggatctccag tattgccagc
    81661 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
    81721 gccggaaggg agattgcggt acgttccagt tcgcaggtga catcttttgg cagaggaggt
    81781 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
    81841 agcagaatcg gatcggtgtc gggatgaata acgccgcgat agctccacgg caacggcgca
    81901 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
    81961 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
    82021 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
    82081 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
    82141 ccggaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggt gagataacaa
    82201 cctgtaacgg catctgccag cgacggcaat tctggcgtgt taatttcctg cgggcattcg
    82261 cgccacattt ttgccagtac cggagcgtgc cagccaccgc agacaactaa cacgtcgcca
    82321 ttattttgct gcatcgccca tccaatccag cgggccataa aggcttcgcg cagacgattg
    82381 agagcatctc cgggggagtc gccccgcagt tgggcaaaat agtgcgccag tgcagagggt
    82441 aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtgtc gctgttatcc
    82501 atgcgggtgg cacgcagcag caaggcctgg ctttcctctt gcgtatcagg cgaatcgtcc
    82561 acttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc ctgaatacga
    82621 cgtgcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt ccaggcacca
    82681 cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac cggaagctgg
    82741 tgggctaaaa acagttcgtc tacccggtca ttaaaatcag ccgggccttc aatcaatacg
    82801 tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc cggactatga
    82861 tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca ggcggtggcg
    82921 ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgactcgtt gttcgaagta
    82981 acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg caatgcagtc
    83041 caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga cgcctacggc
    83101 gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat ccattgacgt
    83161 tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgg accagtaact ccagtaccgc
    83221 gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag cactggcgac
    83281 aagctccagc tcctgggcga aatccataat cggaaagaca gtttcaaaat cgaagcgacg
    83341 ctttagcgca gcactcattt cattgacgcc gcgatctcgg gtattggcag tggcaataat
    83401 attaaagcct tctcgcgcat agagctgact ggcttcacca gtgagttccg gcaccgtcat
    83461 caccctgtca gaaagcatac ctaacagaca gtcttgcact tccagcggtg tacgcgttat
    83521 ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg cagggaccag
    83581 cgcttccgtt gatgggccat gattgataag cagagcgtag ttccagccgt acttgatttg
    83641 atcttcagtg gtagatgcgc cgccctggat ggttaacccg gcatcgccgc taatagcggt
    83701 tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca acatcaaacc
    83761 gcgtccggtg gcaagagtga ctaacatacg ttcgacagcg gagggactaa tcaccacttt
    83821 acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag gcaaacttaa
    83881 ctgccaaccg ggcgggcaag gagcgttatc attttgtttt aattttgcca gttcatcggc
    83941 gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca tatattcatc
    84001 cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt ggcgcattag
    84061 ttgtttgagc aaaaaatatc tggaaagcag atcgcagtaa ataaaaaaac ctgctggtga
    84121 ggcaggttct tttatttact attttcaggc ctaacaccac agcctgtgca acatcgtgtt
    84181 ccaacgaacg aattataacc acggctggac cccgttgcaa ttaagataat gagatttata
    84241 atccagaccg ttgggacgtt gcgccggatc gaacctattt caagtcctgg ctaccggacg
    84301 gggggtgctg caggtggtgc cctgatccgg agtgcaacat cgtggtccaa catacgtaaa
    84361 ccagtatcga caggcagtgt aagggtaagg tgctggtgtt gcgctcctta ataaggagag
    84421 cggatggact cgctgacaca aaagttaacc gtgctcattg ctgtactgga gctattagtg
//
LOCUS       DAFAVF010000003        81603 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFAVF010000003 DAFAVF010000000
VERSION     DAFAVF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.003"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            82..342
                     /gene="iraP"
                     /locus_tag="LGK42_000151"
     CDS             82..342
                     /gene="iraP"
                     /locus_tag="LGK42_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBK2656441.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            443..1858
                     /gene="phoA"
                     /locus_tag="LGK42_000152"
     CDS             443..1858
                     /gene="phoA"
                     /locus_tag="LGK42_000152"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBK2656442.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            1977..2297
                     /gene="psiF"
                     /locus_tag="LGK42_000153"
     CDS             1977..2297
                     /gene="psiF"
                     /locus_tag="LGK42_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBK2656443.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            2399..3514
                     /gene="adrA"
                     /locus_tag="LGK42_000154"
                     /gene_synonym="dgcC"
     CDS             2399..3514
                     /gene="adrA"
                     /locus_tag="LGK42_000154"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBK2656444.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3531..4340)
                     /gene="proC"
                     /locus_tag="LGK42_000155"
     CDS             complement(3531..4340)
                     /gene="proC"
                     /locus_tag="LGK42_000155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK2656445.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            4460..4918
                     /locus_tag="LGK42_000156"
     CDS             4460..4918
                     /locus_tag="LGK42_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK2656446.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5101..5625
                     /gene="aroL"
                     /locus_tag="LGK42_000157"
     CDS             5101..5625
                     /gene="aroL"
                     /locus_tag="LGK42_000157"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBK2656447.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            5675..5866
                     /gene="yaiA"
                     /locus_tag="LGK42_000158"
     CDS             5675..5866
                     /gene="yaiA"
                     /locus_tag="LGK42_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBK2656448.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            6124..6801
                     /locus_tag="LGK42_000159"
     CDS             6124..6801
                     /locus_tag="LGK42_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBK2656449.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            6873..7157
                     /gene="ppnP"
                     /locus_tag="LGK42_000160"
     CDS             6873..7157
                     /gene="ppnP"
                     /locus_tag="LGK42_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBK2656450.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            7361..7780
                     /locus_tag="LGK42_000161"
     CDS             7361..7780
                     /locus_tag="LGK42_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656451.1"
                     /translation="MYELLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(7858..8769)
                     /gene="rdgC"
                     /locus_tag="LGK42_000162"
     CDS             complement(7858..8769)
                     /gene="rdgC"
                     /locus_tag="LGK42_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBK2656452.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            8894..9802
                     /gene="mak"
                     /locus_tag="LGK42_000163"
     CDS             8894..9802
                     /gene="mak"
                     /locus_tag="LGK42_000163"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBK2656453.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(9945..11129)
                     /gene="araJ"
                     /locus_tag="LGK42_000164"
     CDS             complement(9945..11129)
                     /gene="araJ"
                     /locus_tag="LGK42_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBK2656454.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11255..14398)
                     /gene="sbcC"
                     /locus_tag="LGK42_000165"
     CDS             complement(11255..14398)
                     /gene="sbcC"
                     /locus_tag="LGK42_000165"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBK2656455.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFSREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(14395..15597)
                     /gene="sbcD"
                     /locus_tag="LGK42_000166"
     CDS             complement(14395..15597)
                     /gene="sbcD"
                     /locus_tag="LGK42_000166"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBK2656456.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            15787..16476
                     /gene="phoB"
                     /locus_tag="LGK42_000167"
     CDS             15787..16476
                     /gene="phoB"
                     /locus_tag="LGK42_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBK2656457.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16534..17829
                     /gene="phoR"
                     /locus_tag="LGK42_000168"
     CDS             16534..17829
                     /gene="phoR"
                     /locus_tag="LGK42_000168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBK2656458.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            18236..19555
                     /gene="brnQ"
                     /locus_tag="LGK42_000169"
     CDS             18236..19555
                     /gene="brnQ"
                     /locus_tag="LGK42_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBK2656459.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            19631..21004
                     /gene="proY"
                     /locus_tag="LGK42_000170"
     CDS             19631..21004
                     /gene="proY"
                     /locus_tag="LGK42_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBK2656460.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            21160..22977
                     /gene="malZ"
                     /locus_tag="LGK42_000171"
     CDS             21160..22977
                     /gene="malZ"
                     /locus_tag="LGK42_000171"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBK2656461.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(22982..23563)
                     /gene="acpH"
                     /locus_tag="LGK42_000172"
     CDS             complement(22982..23563)
                     /gene="acpH"
                     /locus_tag="LGK42_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBK2656462.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            23656..24726
                     /gene="queA"
                     /locus_tag="LGK42_000173"
     CDS             23656..24726
                     /gene="queA"
                     /locus_tag="LGK42_000173"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK2656463.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24782..25909
                     /gene="tgt"
                     /locus_tag="LGK42_000174"
     CDS             24782..25909
                     /gene="tgt"
                     /locus_tag="LGK42_000174"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK2656464.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            25932..26264
                     /gene="yajC"
                     /locus_tag="LGK42_000175"
     CDS             25932..26264
                     /gene="yajC"
                     /locus_tag="LGK42_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK2656465.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            26292..28139
                     /gene="secD"
                     /locus_tag="LGK42_000176"
     CDS             26292..28139
                     /gene="secD"
                     /locus_tag="LGK42_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBK2656466.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            28150..29121
                     /gene="secF"
                     /locus_tag="LGK42_000177"
     CDS             28150..29121
                     /gene="secF"
                     /locus_tag="LGK42_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBK2656467.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            29250..29597
                     /locus_tag="LGK42_000178"
     CDS             29250..29597
                     /locus_tag="LGK42_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBK2656468.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(29774..30658)
                     /gene="tsx"
                     /locus_tag="LGK42_000179"
     CDS             complement(29774..30658)
                     /gene="tsx"
                     /locus_tag="LGK42_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBK2656469.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(30957..31496)
                     /locus_tag="LGK42_000180"
     CDS             complement(30957..31496)
                     /locus_tag="LGK42_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBK2656470.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            31647..32096
                     /gene="nrdR"
                     /locus_tag="LGK42_000181"
     CDS             31647..32096
                     /gene="nrdR"
                     /locus_tag="LGK42_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBK2656471.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            32100..33203
                     /gene="ribD"
                     /locus_tag="LGK42_000182"
     CDS             32100..33203
                     /gene="ribD"
                     /locus_tag="LGK42_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBK2656472.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            33292..33762
                     /gene="ribE"
                     /locus_tag="LGK42_000183"
     CDS             33292..33762
                     /gene="ribE"
                     /locus_tag="LGK42_000183"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBK2656473.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            33782..34201
                     /gene="nusB"
                     /locus_tag="LGK42_000184"
     CDS             33782..34201
                     /gene="nusB"
                     /locus_tag="LGK42_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK2656474.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            34279..35256
                     /gene="thiL"
                     /locus_tag="LGK42_000185"
     CDS             34279..35256
                     /gene="thiL"
                     /locus_tag="LGK42_000185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBK2656475.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            35234..35752
                     /gene="pgpA"
                     /locus_tag="LGK42_000186"
     CDS             35234..35752
                     /gene="pgpA"
                     /locus_tag="LGK42_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK2656476.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(35806..36780)
                     /gene="yajO"
                     /locus_tag="LGK42_000187"
     CDS             complement(35806..36780)
                     /gene="yajO"
                     /locus_tag="LGK42_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBK2656477.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(36960..38822)
                     /gene="dxs"
                     /locus_tag="LGK42_000188"
     CDS             complement(36960..38822)
                     /gene="dxs"
                     /locus_tag="LGK42_000188"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK2656478.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(38847..39746)
                     /gene="ispA"
                     /locus_tag="LGK42_000189"
     CDS             complement(38847..39746)
                     /gene="ispA"
                     /locus_tag="LGK42_000189"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBK2656479.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(39746..39988)
                     /gene="xseB"
                     /locus_tag="LGK42_000190"
     CDS             complement(39746..39988)
                     /gene="xseB"
                     /locus_tag="LGK42_000190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK2656480.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            40194..41642
                     /gene="thiI"
                     /locus_tag="LGK42_000191"
     CDS             40194..41642
                     /gene="thiI"
                     /locus_tag="LGK42_000191"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK2656481.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(41696..42286)
                     /gene="yajL"
                     /locus_tag="LGK42_000192"
     CDS             complement(41696..42286)
                     /gene="yajL"
                     /locus_tag="LGK42_000192"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBK2656482.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(42249..43160)
                     /gene="panE"
                     /locus_tag="LGK42_000193"
     CDS             complement(42249..43160)
                     /gene="panE"
                     /locus_tag="LGK42_000193"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK2656483.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGAIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            43328..43819
                     /gene="yajQ"
                     /locus_tag="LGK42_000194"
     CDS             43328..43819
                     /gene="yajQ"
                     /locus_tag="LGK42_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBK2656484.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(43947..45311)
                     /locus_tag="LGK42_000195"
     CDS             complement(43947..45311)
                     /locus_tag="LGK42_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656485.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            45719..46654
                     /locus_tag="LGK42_000196"
     CDS             45719..46654
                     /locus_tag="LGK42_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2656486.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(46710..47252)
                     /locus_tag="LGK42_000197"
     CDS             complement(46710..47252)
                     /locus_tag="LGK42_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2656487.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(47236..47547)
                     /locus_tag="LGK42_000198"
     CDS             complement(47236..47547)
                     /locus_tag="LGK42_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK2656488.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(47732..48622)
                     /gene="cyoE"
                     /locus_tag="LGK42_000199"
     CDS             complement(47732..48622)
                     /gene="cyoE"
                     /locus_tag="LGK42_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBK2656489.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(48634..48963)
                     /locus_tag="LGK42_000200"
     CDS             complement(48634..48963)
                     /locus_tag="LGK42_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBK2656490.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(48963..49577)
                     /locus_tag="LGK42_000201"
     CDS             complement(48963..49577)
                     /locus_tag="LGK42_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBK2656491.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(49567..51558)
                     /gene="cyoB"
                     /locus_tag="LGK42_000202"
     CDS             complement(49567..51558)
                     /gene="cyoB"
                     /locus_tag="LGK42_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBK2656492.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(51580..52527)
                     /gene="cyoA"
                     /locus_tag="LGK42_000203"
     CDS             complement(51580..52527)
                     /gene="cyoA"
                     /locus_tag="LGK42_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBK2656493.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(52987..54462)
                     /gene="ampG"
                     /locus_tag="LGK42_000204"
     CDS             complement(52987..54462)
                     /gene="ampG"
                     /locus_tag="LGK42_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBK2656494.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDT
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(54506..55084)
                     /locus_tag="LGK42_000205"
     CDS             complement(54506..55084)
                     /locus_tag="LGK42_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2656495.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            55389..55706
                     /gene="bolA"
                     /locus_tag="LGK42_000206"
     CDS             55389..55706
                     /gene="bolA"
                     /locus_tag="LGK42_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBK2656496.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            56050..57348
                     /gene="tig"
                     /locus_tag="LGK42_000207"
     CDS             56050..57348
                     /gene="tig"
                     /locus_tag="LGK42_000207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK2656497.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            57594..58217
                     /gene="clpP"
                     /locus_tag="LGK42_000208"
     CDS             57594..58217
                     /gene="clpP"
                     /locus_tag="LGK42_000208"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK2656498.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            58343..59617
                     /gene="clpX"
                     /locus_tag="LGK42_000209"
     CDS             58343..59617
                     /gene="clpX"
                     /locus_tag="LGK42_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBK2656499.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            59805..62159
                     /gene="lon"
                     /locus_tag="LGK42_000210"
     CDS             59805..62159
                     /gene="lon"
                     /locus_tag="LGK42_000210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBK2656500.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            62368..62640
                     /gene="hupB"
                     /locus_tag="LGK42_000211"
     CDS             62368..62640
                     /gene="hupB"
                     /locus_tag="LGK42_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBK2656501.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            62832..64703
                     /gene="ppiD"
                     /locus_tag="LGK42_000212"
     CDS             62832..64703
                     /gene="ppiD"
                     /locus_tag="LGK42_000212"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK2656502.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNELAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEVVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            64854..65225
                     /locus_tag="LGK42_000213"
     CDS             64854..65225
                     /locus_tag="LGK42_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK2656503.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            65319..65717
                     /gene="fadM"
                     /locus_tag="LGK42_000214"
     CDS             65319..65717
                     /gene="fadM"
                     /locus_tag="LGK42_000214"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBK2656504.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(65769..66464)
                     /gene="queC"
                     /locus_tag="LGK42_000215"
     CDS             complement(65769..66464)
                     /gene="queC"
                     /locus_tag="LGK42_000215"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBK2656505.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(66529..68229)
                     /locus_tag="LGK42_000216"
     CDS             complement(66529..68229)
                     /locus_tag="LGK42_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBK2656506.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            68329..69147
                     /gene="cof"
                     /locus_tag="LGK42_000217"
     CDS             68329..69147
                     /gene="cof"
                     /locus_tag="LGK42_000217"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBK2656507.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            69300..69758
                     /gene="decR"
                     /locus_tag="LGK42_000218"
     CDS             69300..69758
                     /gene="decR"
                     /locus_tag="LGK42_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBK2656508.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            69788..71560
                     /locus_tag="LGK42_000219"
     CDS             69788..71560
                     /locus_tag="LGK42_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBK2656509.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            71553..73334
                     /locus_tag="LGK42_000220"
     CDS             71553..73334
                     /locus_tag="LGK42_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBK2656510.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            73515..73853
                     /gene="glnK"
                     /locus_tag="LGK42_000221"
     CDS             73515..73853
                     /gene="glnK"
                     /locus_tag="LGK42_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK2656511.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            73883..75169
                     /gene="amtB"
                     /locus_tag="LGK42_000222"
     CDS             73883..75169
                     /gene="amtB"
                     /locus_tag="LGK42_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBK2656512.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(75217..76077)
                     /gene="tesB"
                     /locus_tag="LGK42_000223"
     CDS             complement(75217..76077)
                     /gene="tesB"
                     /locus_tag="LGK42_000223"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBK2656513.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            76295..76867
                     /locus_tag="LGK42_000224"
     CDS             76295..76867
                     /locus_tag="LGK42_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBK2656514.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(76898..77209)
                     /locus_tag="LGK42_000225"
     CDS             complement(76898..77209)
                     /locus_tag="LGK42_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBK2656515.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            77372..77468
                     /gene="ffs"
                     /locus_tag="LGK42_000226"
     ncRNA           77372..77468
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LGK42_000226"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            77588..77941
                     /locus_tag="LGK42_000227"
     CDS             77588..77941
                     /locus_tag="LGK42_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBK2656516.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(77983..79533)
                     /gene="pdeB"
                     /locus_tag="LGK42_000228"
     CDS             complement(77983..79533)
                     /gene="pdeB"
                     /locus_tag="LGK42_000228"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBK2656517.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(79697..80167)
                     /locus_tag="LGK42_000229"
     CDS             complement(79697..80167)
                     /locus_tag="LGK42_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBK2656518.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(80283..80834)
                     /gene="maa"
                     /locus_tag="LGK42_000230"
     CDS             complement(80283..80834)
                     /gene="maa"
                     /locus_tag="LGK42_000230"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBK2656519.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(81006..81224)
                     /gene="hha"
                     /locus_tag="LGK42_000231"
     CDS             complement(81006..81224)
                     /gene="hha"
                     /locus_tag="LGK42_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBK2656520.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(81250..>81603)
                     /gene="tomB"
                     /locus_tag="LGK42_000232"
     CDS             complement(81250..>81603)
                     /gene="tomB"
                     /locus_tag="LGK42_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBK2656521.1"
                     /translation="RHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNEL
                     IEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRKSGNRLFR
                     CFVNATKENPASLSC"
BASE COUNT        19874 a        20161 c        21757 g        19811 t
ORIGIN      
        1 ataaaaataa tacttccaga cactatgaag ttgtgaaaca taatgttaac ttctccatac
       61 tttggataag gaaatacaga catgaaaaat ctcattgctg agttgttatt taagcttgcc
      121 caaaaagaag aagagtcgaa agaactgtgt gcgcaggtag aagctttgga gattatcgtc
      181 actgcaatgc ttcgcaatat ggcgcaaaat gaccaacagc ggttgattga tcaggtagag
      241 ggggcgctgt acgaggtaaa gcccgatgcc agcattcctg acgacgatac ggagctgctg
      301 cgcgattacg taaagaagtt attgaagcat cctcgtcagt aaaaagttaa tcttttcaac
      361 agctgtcata aagttgtcac ggccgagact tatagtcgct ttgtttttat tttttaatgt
      421 atttgtacat ggagaaaata aagtgaaaca gagcactatt gcactggcac tcttatcgtt
      481 actgtttacc cctgtgacaa aagcccggac accagaaatg cctgttctgg aaaaccgggc
      541 tgctcagggc gatattactg cacccggcgg tgctcgccgt ttaacgggtg atcagactgc
      601 cgctctgcgt gattctctta gcgataaacc tgcaaaaaat attattttgc tgattggcga
      661 tgggatgggg gattcggaaa ttactgccgc acgtaattat gccgaaggtg cgggcggctt
      721 ttttaaaggt atcgatgcct taccgcttac cgggcaatac actcactatg cgctgaataa
      781 aaaaaccggc aaaccggact acgtcaccga ctcggctgca tcagcaaccg cctggtcaac
      841 cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt cacgaaaaag atcacccaac
      901 gattctggaa atggcaaaag ccgcaggtct ggcgaccggt aacgtttcta ccgcagagtt
      961 gcaggatgcc acgcccgctg cgctggtggc gcatgtgacc tcgcgcaaat gctacggtcc
     1021 gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa aaaggcggaa aaggatcgat
     1081 taccgaacag ctgcttaacg ctcgtgccga cgttacgctt ggcggtggcg caaaaacctt
     1141 tgctgaaacg gcaaccgccg gtgaatggca gggaaaaacg ctgcgtgaac aggcacaggc
     1201 gcgtggttat cagttggtca gcgatgctgc ctcactgaat tcggtgacgg aagcgaatca
     1261 gcaaaaacct ctgcttggac tgtttgctga cggcaatatg ccagtgcgct ggctaggacc
     1321 gaaagcaacg taccacggca atatcgataa gcccgcagtc acctgtacgc ctaatccgca
     1381 acgtaatgac agtgtaccaa ccctggcgca gatgaccgac aaagccattg aattgttgag
     1441 taaaaatgag aaaggatttt tcctgcaagt tgaaggtgcg tcaatcgata aacaggatca
     1501 cgctgcgaat ccttgtgggc aaattggcga gacggtcgat ctcgatgaag ccgtacaacg
     1561 ggcgctggaa ttcgctaaaa aggatggcaa tacgctggtc atagtcaccg ctgatcacgc
     1621 ccacgccagc cagattgtcg cgccggatac caaagctccg ggcctcaccc aggcgctaaa
     1681 taccaaagat ggcgcagtga tggtgatgag ctacgggaac tccgaagagg attcacaaga
     1741 acataccggc agtcagttgc gtattgcggc gtatggcccg catgccgcca atgttgttgg
     1801 actgaccgac cagaccgatc tcttctacac catgaaagcc gccctggggc tgaaataaaa
     1861 ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt tgctgttagc aaccagactt
     1921 aatggcagat cacgggcgca tacgctcatg gttaaaacat gaagagggat ggtgctatga
     1981 aaataacatt actggttacc ttgctttttg gtctggtttt tttaaccacc gtcggcgctg
     2041 ccgagagaac cttaacccca caacaacagc gtatgacatc ctgtaatcag caggcgacgg
     2101 cgcaggcgtt gaaaggggat gctcgtaaga cctacatgag tgattgcctg aagaacagca
     2161 agtctgcgcc tggtgaaaaa agtttgacgc cacagcagca aaagatgcgc gaatgtaata
     2221 atcaagcaac gcaacaatct ctgaaaggtg atgatcgtaa taagtttatg agtgcctgcc
     2281 tcaagaaagc cgcctgatac ctgatagtgc taacgggtga gctacgaaag tggctcaccc
     2341 gaaatttcat acttctgcct ttagccccgt ctctataatt tgggaaaatt gtttctgaat
     2401 gttcccaaaa ataatgaatg atgaaaactt tttcaaaaaa gcggcggcgc atggggagga
     2461 acctccttta actcctcaaa acgaacatca gcggtccggg ctgcgcttcg cccgtcgcgt
     2521 cagactaccc cgtgcggttg gcctggctgg catgttctta ccgattgctt caacgctggt
     2581 ttcgcacccg ccgccgggct ggtggtggct ggtgttggtc ggctgggcgt tcgtctggcc
     2641 gcatttagcc tggcagatag cgagcagggc cgtcgatccg cttagccggg aaatttacaa
     2701 cttaaaaacc gatgcagtat tagcgggaat gtgggtaggc gtaatgggcg taaacgtgct
     2761 gccttccacc gctatgttga tgattatgtg tctgaatttg atgggggcag gcggcccccg
     2821 tctgtttgtc gcgggtctgg tgttgatggt ggtttcctgc cttgtcaccc tcgagcttac
     2881 gggcattacc gtgtcgttca atagtgcgcc gctggaatgg tggctctccc ttcccattat
     2941 tgtcgtttat cctctgctgt ttggctgggt cagctaccag acggcaacca aactggcgga
     3001 acataaacgc aggttgcagg tcatgagtac ccgcgacggc atgacgggcg tgtataaccg
     3061 acgtcattgg gaaactatgt tacgcaatga atttgataac tgtcggcggc ataatcgcga
     3121 tgcaacgtta ctgattatcg atatcgacca tttcaagagc atcaacgata cctggggtca
     3181 tgatgtgggc gatgaagcga ttgtggcgct tacccgacag ttacaaatta ccctgcgcgg
     3241 tagcgatgtg attggtcggt ttggcggcga tgagtttgca gtaatcatgt ccggtacgcc
     3301 agctgagagc gccattaccg ccatgttacg ggtgcgtgaa gggctaaaca cgttacgctt
     3361 gccgaatacg ccacaggtaa ctttacggat tagtgtgggg gttgcgccgc tgaacccaca
     3421 aatgagtcac tatcgtgagt ggttgaaatc ggcagatttg gcgctttaca aagcaaagaa
     3481 agccggacgt aaccgcaccg aagtggcggc ctgacgtccg gcgaaagtca tcaggatttg
     3541 ctgagttttt ctgatttttc catacacttc gtcatcgctt cgatcactgc ggcacggaag
     3601 cctttctctt ccagtacgcg taccgcttca atggtggtgc ctcccggtga gcagaccata
     3661 tctttcagtg cccccggatg ttctcccgtt tccagcacca tttttgcgga acccattacc
     3721 gcctgagcgg caaatttata cgcctgggcg cgtggcatcc cgcccagcac ggcggcgtcg
     3781 gccatcgctt cgataaacat aaatacgtag gctggcgaag aaccgctcac accgaccacc
     3841 gggtggatca tcggctcagc aattacttcc gcttcgccaa agcagcggaa aatattcagc
     3901 acatcagcgg tatcttctgg ggttaccagc gcgtttggcg ttacggaggt catcccggca
     3961 ttaaccagcg cgggagtgtt cggcatggcg cggataattt ttcggtcatg gcccagcgcg
     4021 cgggcaagtt ggtcgagcgt gacacctgca gcaatagaaa cgaccagaga gtctttattc
     4081 aggctggagg tgatttcgct aagcacttta atcatgatgc caggtttaac ggcagcaaaa
     4141 atgatgtcgg cgatttgcgc cacttcttgc gccgattctg cggcgttgat gccgaactgg
     4201 tcatgcaggg cggcgacttt atccggggag ggggtgtata cccagatttg ccctggaagc
     4261 acctgaccgc tggcaatcag accgccgaga atggcttttc ccatattgcc gcagccaata
     4321 aaaccgattt tcttttccat tgcctcactc ctgccgtgaa attcattgtt ttgataatcg
     4381 ctggcagaag cataaacaga actatgccgg aaggcaaaag cgcgacacaa tagagaatta
     4441 cccaacaaag gatgacttta tgacaatttg ggtggatgcc gacgcgtgtc ccaatgtaat
     4501 taaagagatt ttgtatcgcg cggcggaacg tatgcagatg ccgctggtac tggtagcaaa
     4561 ccagagttta cgcgtgccgc catcgcgatt tattcgtacg ctgcgcgtcg cggcaggttt
     4621 cgacgttgcc gataacgaaa ttgtccggca gtgtgaagcg ggcgatttgg tgatcaccgc
     4681 agatatacct ttggctgctg aagccatcga gaaaggcgca gcggcgctta atccgcgcgg
     4741 cgaacgttac acgccagcga ccattcgtga gcgcctgacg atgcgcgatt ttatggatac
     4801 cttacgtgcc agtgggatcc agaccggcgg accagatagc ctttcacaac gtgaccgcca
     4861 ggcctttgcc gcggagctgg agaagtggtg gctggaagtg caacgtagtc gtggctaaat
     4921 gtaatttatt atttacactt cattcttgaa tatttattgg tatagtaagg gatgtattga
     4981 gattttcact ttaagtggaa ttttttcttt acaatcgaaa ttgtactagt ttgatggtat
     5041 gatcgctatt ctcatgacac cggctttcgc cgcattgcga cctattgggg aaaacccacg
     5101 atgacacaac ctctttttct gatcgggcct cggggctgtg gtaaaacaac ggtcggaatg
     5161 gcccttgccg attcgcttaa ccgtcggttt gtcgataccg atcagtggtt gcaatcacag
     5221 ctcaatatga cggtcgcgga gatcgtcgaa agggaagagt gggcgggatt tcgcgccaga
     5281 gaaacggcgg cgctggaagc ggtaactgcg ccatccaccg ttatcgctac aggcggcggc
     5341 attattctga cggaatttaa tcgtcacttt atgcaaaata acgggatcgt ggtttatttg
     5401 tgtgcgccag tatcagtcct ggttaaccga ctgcaagctg caccggaaga agatttacgg
     5461 ccaaccttaa cgggaaaacc gctgagcgaa gaagttcagg aagtgctgga agaacgcgat
     5521 gcgctatatc gcgaagttgc gcatattatc atcgacgcaa caaacgaacc cagccaggtg
     5581 atttctgaaa ttcgtagcgc cctggcacag acgatcaatt gttgatttta gagcgcctat
     5641 acttaacgtt catcccgtga aataaggaag aacgatgcca acgaaaccgc cttatcctcg
     5701 tgaagcatat atagtgacga ttgaaaaagg aaagccagga cagacggtaa cctggtacca
     5761 actcagagcc gatcatccta aaccagactc gttgatcagt gaacatccga cggctcagga
     5821 agcgatggat gcgaaaaaac gctatgagga ccctgacaaa gagtgaccgc atcagactgc
     5881 tcggaaggga ttctgagtgc cactacaagg gatctgcgtc acatttttca taattcatgt
     5941 ttttctaata attagaatat taaacaataa caatccatta ctggaatcat ttggaatctt
     6001 tacattatgc cgtgcacgtc tgctgctacg ctttttgtca tttgtagcac aagtaagtgt
     6061 cagcagtggt gcttcacact tgcccggtaa ttaacgacga aagaaaagta aggtggatga
     6121 acaatgagtg cgtcgttggc gatcctgacc atcggcattg tacctatgca ggaagttttg
     6181 ccgctcctga cggaatacat tgacgaagat aatatttccc atcatagcct gctggggaag
     6241 ttaagtcgtg aagaagtgat ggcggagtac gcgccagaag caggcgaaga caccattctc
     6301 acattattaa atgacaacca gctggcccat gtttcgcgtc gcaaagtgga gcgtgacctg
     6361 caaggtgtgg ttgaagtact cgataatcgg ggttacgacg tcattatatt aatgagtacg
     6421 gcaaacatta gtagtatgac tgcgcgtaat acgatctttc ttgagccgtc gcggatattg
     6481 ccaccgctgg tttcctctat tgttgaagat catcaagtgg gggttatcgt tccggttgag
     6541 gagatgctgc ccgttcaggc gcaaaaatgg caaattttgc agaaatcgcc ggtattttca
     6601 ttgggtaacc ccattcatga ttcagaacaa aaaatcattg atgccgggaa agaattactg
     6661 gcaaaagggg ctgatgtcat catgctggat tgtttaggat ttcaccaacg tcatcgcgat
     6721 ttactgcaaa aacagctcga tgttcctgtc ttgctgtcta acgtattgat tgcacggctg
     6781 gctgcggaat tactggtgta attttgcgtg acagtcagcg cctctggccc ctatagtgaa
     6841 gtagatgttc aactaccaga cagggccagt ttatgcttca aagtaatgag tacttttccg
     6901 gcaaagtgaa atcaatcggc ttttccagca gcagcactgg tcgcgccagc gtgggtgtta
     6961 tggttgaagg cgaatacacc ttcagcaccg ctgagccgga agagatgacg gtaatcagtg
     7021 gcgcgctgaa tgtgttactg cctgacgcga ccgactggca ggtgtatgaa gccggttcgg
     7081 tgtttaatgt tcccggtcac agtgagtttc atctgcaagt tgccgaaccc acctcttatc
     7141 tgtgccgcta tctgtaattc ctcgccttcc ccttgaacgg gagggcattt ttctgaaata
     7201 tcctctcttt aacccataat aatatttcct ttgcttcgat tttttcaatt tccgatatat
     7261 tcataattca tcaaggttga tataaatatc agtgaagatc tccaggtatt ttttgcggaa
     7321 ctgatgacac aacgccccct ggagcaagtt acagcgggag atctatgaat tactgacgcc
     7381 aacgatcaac ctgcaaattc actgtacccg ttatcccatg cgcagccaaa atggtggaag
     7441 caccgattta ccgcgttatt ggatcacgtt ggataaaaac gtgatctggg attacccgaa
     7501 agactttatt gcaggtaacg gtggagtgcg taattttcac ggtgaaactt gctggtatcc
     7561 ttacctgacg gatatttgtt caatttctga tttattgcgt gaatatattg acaccccaaa
     7621 agcggagttg ttaacaaaac aatttaccag cgataaatgg gggttagtga atattctgcg
     7681 tgctgctgac aggcgaatag ggatgaggcg attagatcag ttacggcgta aaacgcataa
     7741 tattgctgcg ctaaaaatta ttgcccgccg tagcgaataa ttatatgcct ggtgtggcat
     7801 cgtacgccgg ataagacgcg gcaggcgtcg catccggcat taaaggaaaa tcagcaatta
     7861 acgttgtgct tcgccaccta atccttcaat caggttttga attaacgctg ccagttcacc
     7921 agtcatcagg atgaaatcgg catcaaaacg ctgggcgaaa tcttcacggt cgatatcttc
     7981 gttttgatcg cgcagctcgt cgcagaactt cagacgcttg agcgaaccat cgtcgcacat
     8041 cacaaactga atgcgctgct gccagtcgag cgccagttta gtcaccactt ttccggcttc
     8101 gatgtgattg gtgatctctt cgctggtcag atcttgtttc ttcgcacgga tcacgccgcc
     8161 atcttccagc aacgatttca gctcggcttc atcaagcaac tggaagccct gtgctgcgct
     8221 accggagcga acccattcgg ttagcgtcag ttcaatcggg ttttccatgc tcaatggtac
     8281 aacaggtaac gaccccaggc ttttacgcag taatgccagc gtatcttccg cttttttggc
     8341 gctggcgcag tccaccataa tcaaaccatt aaccgtgtcg atccacatca ttgtctggct
     8401 aaaacggctg aaagctcgcg gcagcagaga gtgcagcact tcgtctttca gcgaatcttt
     8461 ttcggttttc ttcagcttac gcgcctgttc cgcttccagt ttggcgattt tcgcttccag
     8521 cgcctgttta atcaccggag acgggaggat tttttcttct ttgcgtgcgc agataacaat
     8581 ttgaccattg gcaacgtgcg ttaacgcatc gctgtgcgat cccatcggag gaacccagcc
     8641 catcttcgcc atgtcctggc tgccacatgg ggtaaatgcc atcgaggcta gctgtttttc
     8701 catctcttct gcacgcagcg aaatctcgcg gctaagacgg taaaccatta aatttttgaa
     8761 ccacagcatg ataatttcca cggccttgtc gttaaattta gcgggcatga taacgaattg
     8821 tcggcggcct tgcattgcca atccggttgt ccgtctctac gctattgata ttgaaaaaaa
     8881 taaggagagt accgtgcgta taggtatcga tttaggcggc accaaaactg aagtgattgc
     8941 actgggcgat gcaggggagc agttgtaccg ccatcgtctg cccacgccgc gtgatgatta
     9001 ccggcagact attgaaacga tcgccacgtt ggttgatatg gcggagcagg cgacagggca
     9061 gcgcggaacg gtaggtatgg gcattcctgg ctcaatttcg ccctacaccg gcgtggtgaa
     9121 aaatgccaat tcaacctggc tcaacggtca gccattcgat aaagacttaa gcgcgcggtt
     9181 gcagcgggaa gtgcggctgg caaatgacgc caactgtctg gccgtttcag aagcagtaga
     9241 tggcgcggca gcgggagcac agacggtatt tgccgtgatt atcggcacgg gatgcggcgc
     9301 gggcgtggca ttcaatgggc gggcgcatat cggcggcaat ggcacggcag gcgagtgggg
     9361 acacaatccg ctaccttgga tggacgaaga cgaactgcgt tatcgcgagg aagtcccttg
     9421 ttattgcggt aaacaaggtt gtattgaaac ctttatttcg ggcacgggat ttgccacaga
     9481 ttatcgtcgt ttgagcggac atgcgctgaa aggcagtgaa attatcagcc tggttgaaga
     9541 aagcgatccg gtagcggaac tggcattgcg tcgctacgag ctgcggctgg caaaatcgct
     9601 ggcacatgtc gtgaatattc tcgatccgga tgtaattgtc ctggggggcg ggatgagcaa
     9661 tgtagaccgt ttatatcaaa cggttgggca gttgattaaa caatttgtct tcggcggcga
     9721 atgtgaaacg ccagtgcgta aggcgaagca cggtgattcc agcggcgtac gcggcgctgc
     9781 gtggttatgg ccacaagagt aaaaaaacca ggcaattggc gcatcatgcc tgatgcgacg
     9841 cttgccgcgt cttatcaggc ctacaaaatg tgccagaacc gcgtagggcg gataaggcgt
     9901 tcacgccgca tccggcaata agcaacgcgc accgtgacag taatctaccc cagtggtttc
     9961 gccagcaccg gactatccgc cgcttgctgg cgcttatagc gaccatacag cagcaacgat
    10021 gacatcgcag caaacgaaag cagggcggca ggcagcgcca cgtaattata tgccagcccc
    10081 agcgtcagca tcataccacc gcaatatgcg ccgacggcgc taccgaggtt aaacgctatt
    10141 tgcccacctg cggcacctaa taactctccg cctttggcat tttgcagtaa caatatttgc
    10201 agaggtgctg aaagggcaaa taatcccgcg caacaaataa aagcaaaaat aagcgacgtt
    10261 gttttcatgc cgccgcagaa aaagagcatc agcagtgcca gtacaattat aaagtcagtc
    10321 actgctgcaa tgcgcagtgg tgaataacgt cctgaaatcc tgccacttag catatttccc
    10381 aacaccatcc ctagcccaac taacatcata ataaaggtca tcgccgtttc cgaaaaaccg
    10441 gaaataaaca tcatgtatgg ctttacgtag ctgaaccagg caaacacacc tgcgttgcca
    10501 aacatcgtgg cggcgaaaat taaccacggg gccgggctgc gtaaaaagtg aaattgttcg
    10561 cgcagctttc ctttcgcctc gtcgcgaata tccggcaccc aaaaatagac cgatgccatc
    10621 accgcaatat taaaaacagc gatcaataaa aaggtgtaac gccagctaaa ttcctgactt
    10681 aaatacgttc ccagcggaat gcccagcaaa ttggcgactg tcatcccgga aaccatcccc
    10741 gccacggcgg cggtgacttt tccgggtttg ataatttttg ataacacgat cgcgccgacg
    10801 ccaaaaaatg cgccatgcgg aaagccggaa accaggcgac caatggcaag catcaggtaa
    10861 gacgaagaga gcgtgaacat ggcgttgcca atgacgcaca acgccaccag aaacaacaag
    10921 atatgtttga gtgagtagcg gctggaaaag agtgcgatga ttggcgcacc gaccaccact
    10981 cccagcgcat aatacgagat catatgcccg gcagcaggaa tcgaaattcc tacgttatgc
    11041 gccagctccg tgagcacgcc cataatgcca aattcggcca tccccaaacc aaacgtgccc
    11101 agagccaaag ataaaatgac ttttttcata ccaccatcac aactaccaga acggctacca
    11161 atcaccgtag ccaataaagt gatagcttgc aggataacga ccagcaaggc caagagataa
    11221 ttcgcccctc tgtattcatt atcctgctga atagttattt cactgcaaac gtactttcca
    11281 gtttgctgta gcccaggccg ttgatctttt tcactttgat ctgcaccgga atacgctctt
    11341 tcatcgcttc tacgtggcta atcacaccga tggttttgcc actggcgttc agggcatcca
    11401 gcgcatcaag ggcggtatcc agcgtttcgc tatccagcgt gccaaaacct tcatcaagga
    11461 acagcgagtc aatacgtgtt ttatggctga ccagatccga aagcgccagc gccagcgcca
    11521 gactaacgag gaaactttcg ccgccggaaa gggtacgggt atcgcgtacc gcatctgcct
    11581 gccaggtatc aacaacctcg acttccagcg cctcgctggc tttgcgctgt aacagatagc
    11641 gcccgtgcag ccgggtaagt tgctgattag cgagatggac taaattatcc agcgtcagcc
    11701 cctgggcaaa cttgcggaat ttatcgccct ctttggaacc tattagcgaa ttcagatatc
    11761 cccagtcctc aacctgctgc gtcatttgag caatttgctg cattaaggtt tgttgttgct
    11821 gacggttatc tgcatcctgc ttcagctgct ggcgaatctc gccttgactc gtggtgtttt
    11881 cacgcaactt ttggtgagtt tgcgctaact cttgctgaat ctgctccacc gtcacagtga
    11941 gagccaaccc gtcaggtcgg tgttgttgat gctgtgccag cgtttctgct gtctgagtga
    12001 ccagcgtttg cgcctgacgg cgctggtttt ccagattctg cttgagctgt tccagctgcg
    12061 ttagtgtttg ttcatccatt agcgccgcaa ggaacgcctg ctgatcgtca aagacgctgg
    12121 cctgtagcgc ggtgtcaaac tgcgcctggg ctttttgcag actttgcgcc gccagaacat
    12181 cctgttgctg taacgtctgc tgctggctgt gtaatgcgag acattgttca tgaacctgcc
    12241 gccagttatc cagcgccaca gtttcttcgc tgtgcgggag atcatcactt tgcggcaacg
    12301 tttccagaat cggcgtcagc tgctgaatac ggttttgcag cgcggttaat tcgttctggc
    12361 gttgctgcca gctctgcgct tcttgctgac gtgtcgccaa ccagctctct tcttcatctt
    12421 cctgtggcaa tgtcagtgca taacccgcca atgccgttaa gagctgctgc tggcgttgtt
    12481 caatttgctg ttgatactgg ataatttgct gattatgcgc ggcaatctgc ccttgtaatt
    12541 catgtcgttg gctgagtaac cgcagctggc gttcgtgctc atcttgtgca tccagccacg
    12601 gttgaatatc gtcctgtggc tgcaaggtga tattgaggct ggccgtgacg gcttgccatt
    12661 gttgagtaag tgcttgctca tcttgtcgga ggctttgcgc ttcgttttca tcgcgctgaa
    12721 gctgctttgt taaggcgtcc agttgcccgc gcagcgccgc accttcttcg ccgagctttt
    12781 taacttcgtt ttccagcgcc agtaatcgag actgattaac accaggctcc agcgcctgat
    12841 acgcctcgac cgccgggtgg ctggtggaac cacaaagtgg acaaggctga cccgcctgta
    12901 actgtgcacg ttgagcttcc agcgttttga tgcgcgcttc ctgctcgcaa atggttttca
    12961 catcggcaag ttgctgcgtc ttttctttat aacgctggcg catttcgtta agtgcggcgt
    13021 tacgttgcgt ctgttcttgc gtgacattct ggatagcgac ctgtaactgc gccagacgtt
    13081 tttgttgggg aacaatctgt ccatgtagcg cgaccaggtg ctgacgcagt gggcgttgct
    13141 cagcatgttg cgccagggcg gtagcaactt catcggcggt taacgtcaac gtgatcgccg
    13201 caagcgcatt aagtttttgc tcagcatggg ttaactgttg ctgccattgc cgcagatgct
    13261 cgcgatcgct ggtttgttgg gagaactgcg cacgccaacc cgccagttcg ttgttccact
    13321 gacggaagcg gtcgtgttcc tgtaaccagg tattcaggct ttgttgctgc tgctgtaatt
    13381 ctgctgactg cttcgccgcg tggtggcgaa tgctcgcgcg aagcgccatt gtgctctgta
    13441 agcgagtatt tacttcttca atctgctggc gagtatgcgc cagcgccgcg ctgtgttctg
    13501 cgatgcgttc ccagtgcgga cgaagatttc gtgccggttg tgccagactg agcgccgcca
    13561 gttgaggttg cgctttttct tcttcggcta acgcctgttg caaggcctgc tgacggcggc
    13621 tggcttcttg ctgcaattcg tccagacgcg ttaaccagtt tagtgattgt tgttcttgct
    13681 gctgcgcggt aagtaactgt ttttcttcgt cagtaagtac ctgcaaactc gctgtcagcg
    13741 attgcacttg ttccggcgtg agcaacgcga cgccgctggc ctgcgcttgc agcttctcca
    13801 gctcagtgcg ggccgatttg tgctgctcaa aaaccatcgc cgagatttgc ccgtagattt
    13861 cagtgccggt taactcctcg agcaattccg cgcgttcttt gggtttggca ttcaggaagg
    13921 cagcaaattg cccctgcgaa agcagcatcg aacgggtgaa gcgcccgtaa tccagcccgg
    13981 ttaacgttgc tgtcagttct agcttatctt tcactttgtc ggcgagaatt ttgccgtcgg
    14041 cgcagcgcgc cagctcaacg cgtggcacct gcaaattacc gtcgggttgg ttacgcgccc
    14101 gattctggct ccagaatgca cggtacgctt cacctttcac ttcaaactcc acctccgcca
    14161 gacattcagc ggtatcgcgg gtcatgagat cattttgcga ttgtgaaacg ttagagagac
    14221 gcggagtttc gtgatacagc gccagacaaa tggcgtccag cagggtggtt ttccccgcac
    14281 cggttgggcc ggtaatggca aacagcccgt tgctggcgaa cggctcgcgg ctgaaatcaa
    14341 tcttccattc gccttttaat gagttcaggt ttttcaggcg caggctgaga attttcatgc
    14401 ttcgtgttct ccggcgaggg tatgcaacgt cgtggtgaaa agatgctgca gacgttgctg
    14461 ctgcgattca tccagttctt ccagtgccag acggcgattg aacacctctt cgacgctgag
    14521 ttcgctgagg gtttcacgct gttggctggc taacacacgc tcgcgctgtt cacgactccg
    14581 gcgtaccagc aatacttcaa caggcaatga ttcggttaat gcctggattt tgcgctgaat
    14641 atcatgcaga tactcatcag tagtgatttc gatatccagc cagacaggcg gctcctgtga
    14701 tacatcgcgc cactgttcca gctgtgcggt aatcgacgcc agatcgcctt tcagcaccgc
    14761 catgggttgc gttaccggta cgttcaggtt ttccacgctc tctaatttgc cgtttgaaaa
    14821 tgtcaccaga tggacatatt tactctttcc gcattcatca aaactcagtg gaatgggaga
    14881 gccgcaatag cgaacatgtt ccatgccgcc aataatctgt gcgcggtgaa tatgcccgag
    14941 cgcgatgtag tcggctggtg gaaagttttg tgctggaaac gcgtccagcg tgccaatata
    15001 aatgtcacgc acggcgtcac ttttactggc acccacggtc gttaagtgtc ccgtggcgat
    15061 gatgggcaga ggctgatcgc cgcgcagttt gcaggcatcg gcatagtgtt gttggtaata
    15121 atcggtaatc gctgccagta aatgctgctg tttttcaata ccgttaagcc ccgcctggct
    15181 ggtaataatg tcacgcggac gtaaaaacgg aatggggcac agcactgcgc ctggcgtccc
    15241 gtcgcgacga ggcaagattt gcggcgcatg tccggcgctg gcgaccacgg tcgtattgag
    15301 gaacgccatg atatcgcgcg attcattcag cgtggcgacc gagtcatggt ttcctgccag
    15361 taccaccaga tgacagccag tttgctgtaa attgacaaca aaacggttgt ataacgtgcg
    15421 ggcgtaactg ggcggcgagc cggtatcgaa aacatcacca gcaacaataa tcgcatccac
    15481 ctgatgggtt tgtgctgttt ccagcagcca gtcaagaaaa gcctgatgtt cagcttcgcg
    15541 gcttttactg tagaagttct ggccgagatg ccagtctgag gtgtgaagga tgcgcataac
    15601 gattccctgg cgaaaaagca tgggcgcgat tatacccaaa cagatgtgcc atttgctttt
    15661 ttctgcgcca cggaaatcaa taacctgaag atatgtgcga cgagcttttc ataaatctgt
    15721 cataaatctg acgcataatg acgtcgcatt aatgatcgca acctatttat tacaacaggg
    15781 caaatcatgg cgagacgtat tctggtcgta gaagatgaag ctccaattcg cgaaatggtc
    15841 tgcttcgtgc tcgaacaaaa tggctttcag ccggtcgaag cggaagatta tgacagtgct
    15901 gtgaatcaac tgaatgaacc ctggccggat ttaattctcc ttgactggat gttacctggc
    15961 ggctccggta tccagttcat caaacacctc aagcgcgagt cgatgacccg ggatattcca
    16021 gtggtgatgt tgaccgccag aggggaagaa gaagatcgcg tgcgcggcct tgaaaccggc
    16081 gcggatgact atatcaccaa gccgttttcg ccgaaggagc tggtggcgcg aatcaaagcg
    16141 gtaatgcgcc gtatttcgcc aatggcggtg gaagaggtga ttgagatgca gggattaagt
    16201 ctcgacccga catctcaccg agtgatggcg ggcgaagagc cgctggagat ggggccgaca
    16261 gaatttaaac tgctgcactt ttttatgacg catcctgagc gcgtgtacag ccgcgagcag
    16321 ctgttaaacc acgtctgggg aactaacgtg tatgtggaag atcgtacggt cgatgtccac
    16381 attcgtcgcc tacgtaaagc actggagcca ggcgggcatg accgcatggt gcagaccgtg
    16441 cgcggtacag gatatcgttt ttcaacccgc ttttaacgcc ttgctcatcg gacgcagagc
    16501 agggcttatg atttcttaac tggagtatct tacgtgctgg aacggctgtc gtggaaaagg
    16561 ctggtgctgg agctgctact ttgctgcttc ccggctttca tcctgggtgc attttttggt
    16621 tacctgccct ggtttttgct ggcatcggta acgggactgc ttatctggca tttctggaat
    16681 ttattgcgcc tttcatggtg gctgtgggtg gatcgtagta tgaccccgcc accggggcgt
    16741 ggtagctggg aaccgctact atacggctta caccagatgc agctgcgaaa taaaaaacgc
    16801 cgccgtgaac tgggcaatct gattaaacgc tttcgtagcg gcgcggagtc gctgcccgac
    16861 gcagtggtgc tgaccactga agagggcggt attttctggt gtaacggtct ggcgcaacaa
    16921 attcttggtt tgcgctggcc ggaagataac gggcagaaca tccttaacct gctgcgttac
    16981 ccggagttta cgcaatatct gaaaacgcgt gatttttctc gcccgctcaa tctggtgctc
    17041 aacaccgggc ggcatctgga aattcgcgtc atgccttata cccacaaaca gttgctgatg
    17101 gtggcgcgtg atgtcacgca aatgcatcaa ctggaagggg cgcggcgtaa cttttttgcc
    17161 aacgtgagcc atgagttacg tacgccattg accgtgttac agggttacct ggagatgatg
    17221 gatgagcagc cgctggaagg cgcggtacgc gaaaaagcgt tgcacaccat gcgcgagcag
    17281 acccagcgga tggaagggct ggtgaagcag ttgctgacgc tgtcgaaaat agaagccgca
    17341 ccgacgcatt tgctcaatga aaaggttgat gtgccgatga tgctgcgcgt tgttgagcgt
    17401 gaggctcaga ctctgagtca gaaaaaacag acatttacct ttgagataga taacggcctc
    17461 aaagtgtctg gcaacgaaga tcagctacgc agtgcgattt cgaacctggt ctataacgcc
    17521 gtgaatcata ctccagaagg cacgcatatc accgtacgct ggcagcgagt gccgcacggt
    17581 gccgaattta gcgttgaaga taacggaccg ggcattgcac cggagcatat tccgcgcctg
    17641 accgagcgtt tttatcgcgt tgataaagcg cgttcccggc aaaccggcgg tagcggatta
    17701 gggttagcga tcgtgaaaca tgcggtgaat catcacgaaa gtcgcctgaa tattgagagt
    17761 acagtaggaa aaggaacacg tttcagtttt gttatcccgg aacgtttaat tgccaaaaac
    17821 agcgattaat ccgcctttgt catcttttat tgccataagc cagccgatgc tggcttattt
    17881 tctttgcagt caaaatacgg gcgttagatt ttacaacggt tggtgatttt ttgttcgcat
    17941 gattagccat gtctttttca cggaaatagt gttttatact ggttggtgat ttcttatcgc
    18001 tatatacctc tggtttttag atccctcctt gctttaaaac gttataagcg tttaaattgc
    18061 gcttcaggtg ctgtcatact gactgcatta acgcggtaaa tcgaaaaact attcttcgcc
    18121 gcgcctggtt gggagtattt cccgctaaaa ttgtttaaat ataccgctgt atcatcccca
    18181 gggattggca caaaaattta acgttacaac accacatcca caggcagtat gatttatgac
    18241 ccatcaatta agatcgcgcg atatcatcgc tctgggcttt atgacatttg cgttgttcgt
    18301 cggcgcaggt aacattattt tccctccaat ggtcggcttg caggcaggcg aacacgtctg
    18361 gactgcggca ttcggcttcc tcattactgc cgttggtctg ccggtactaa cggtagtggc
    18421 gctggcaaaa gttggcggcg gtgttgacag cctcagcacg ccaatcggta aagtcgctgg
    18481 cgtactgctg gcaacggttt gttacctggc ggtggggccg cttttcgcta cgccgcgtac
    18541 agctaccgtt tcctttgaag tggggattgc gccgctgact ggtgattccg cgctgccgct
    18601 gtttatctac agcctggtct atttcgctat cgttattctg gtttcgctct atccgggcaa
    18661 gctgctggat accgtgggca acttccttgc gccgctgaaa attatcgcgc tggtcatcct
    18721 gtctgttgcc gcgattgtct ggccggcggg ttctatcagc acggcgactg aggcttatca
    18781 aaacgctgcg ttttctaacg gcttcgttaa cggctatctg accatggata cgctgggcgc
    18841 aatggtgttt ggtatcgtta ttgttaacgc ggcgcgttct cgtggcgtta ccgaagcgcg
    18901 tctgctgacc cgttataccg tctgggctgg cctgatggcg ggtgttggtc tgactctgct
    18961 gtacctggcg ctgttccgtc tgggttcaga cagcgcgtcg ctggtcgatc agtctgcaaa
    19021 cggcgctgct attctgcatg cttacgttca gcacaccttt ggcggcggcg gtagcttcct
    19081 gctggcggcg ttaatcttca tcgcctgcct ggtaacggca gttggcctga cctgtgcttg
    19141 tgcagaattc tttgcccagt acgtaccgct ctcttatcgt acgctggtgt ttatcctcgg
    19201 cggcttctcg atggtggttt ctaacctcgg cttaagccag ctgatccaga tctccgtacc
    19261 ggtgctgacc gctatttatc cgccgtgtat cgcactggtt gtattaagtt ttacacgctc
    19321 atggtggcat aattcgtccc gcgtgattgc tccgccgatg tttatcagcc tgctttttgg
    19381 tattctcgac gggatcaaag catctgcatt cagcgatatc ttaccgtcct gggcgcagcg
    19441 tttaccgctg gccgaacaag gtctggcgtg gttaatgcca acagtggtga tggtggttct
    19501 ggccattatc tgggatcgcg cggcaggtcg tcaggtgacc tccagcgctc actaaatcac
    19561 tgaacatttg ttttaaccac ggggctgcga tgccccgtgg ttttttattg tgttgatggg
    19621 ttaggaattg atggaaagta agaacaagct aaagcgtggg ctaagtaccc gccacatacg
    19681 ctttatggca ctgggttcag caattggcac cgggctgttt tacggttcgg cagacgccat
    19741 caaaatggcc ggtccgagcg tgttgttggc ctatattatc ggtggtatcg cggcgtatat
    19801 cattatgcgt gcgctggggg aaatgtcggt acataacccg gcagccagct ctttctcgcg
    19861 ttatgcgcag gaaaacctcg gcccgctggc aggttacatt accggctgga cctattgctt
    19921 tgaaatcctt attgtcgcca tcgccgatgt gaccgctttt ggtatctata tgggtgtctg
    19981 gttcccgacg gtgccgcact ggatttgggt actgagcgtg gtgctgatca tttgcgccgt
    20041 aaacctgatg agcgtgaagg tcttcggtga gctggaattc tggttctcgt tctttaaagt
    20101 cgccaccatc atcatcatga ttgtcgccgg tttcggcatc atcatctggg ggattggcaa
    20161 cggcgggcaa ccgaccggta ttcataacct gtggagcaac ggcggcttct tcagtaacgg
    20221 ctggcttggg atggtgatgt cgttgcaaat ggtgatgttt gcttacggtg ggatcgaaat
    20281 tatcgggatt accgccggtg aagcgaaaga tcctgaaaaa tcgataccgc gtgcgattaa
    20341 ctccgtgccg atgcgtattc tggtgttcta cgtcggtacg ctgttcgtca ttatgtctat
    20401 ctacccgtgg aatcaggttg gcactgccgg tagcccgttc gtgctgacgt tccagcatat
    20461 gggcattacc tttgccgcca gcattcttaa ctttgttgtg ctgactgctt cgctgtcggc
    20521 aattaacagt gacgtatttg gcgtaggccg tatgctccac ggtatggcag agcagggcag
    20581 cgcgccgaaa attttcagca aaacgtcgcg tcgcggtatt ccgtgggtta cggtgctggt
    20641 gatgactacc gcgctgctgt ttgcggtgta tctgaactac atcatgccgg aaaacgtctt
    20701 cctggtgatc gcttcgctgg caaccttcgc cacggtgtgg gtgtggatta tgatcctgct
    20761 gtcgcaaatt gccttccgtc gccgtttgcc gccagaagaa gttaaggcgc tgaaatttaa
    20821 agtgccgggt ggggtagcaa cgaccatcgg cggtttgatt ttcctgctct ttattatcgg
    20881 gttgattggt tatcacccgg atacgcgtat ctcgctgtat gtcggtttcg cgtggattgt
    20941 tgtgctgttg attggctgga tgtttaaacg ccgccacgat cgtcagctgg ctgaaaacca
    21001 gtaatccttg cgttctgtaa gccggttcag gaggaaatcc tgatccggct attccgaaag
    21061 ttatccgccc ccgtcctcct cccccaaata tccttcagat gatgagtgat cctgcattag
    21121 gctatggcaa ggtgatcaga ttttcatcac aggggaatta tgatgttaaa tgcatggcac
    21181 ctgccggtgc ccccatttgt taaacaaagc aaagatcaac tgctcattac actgtggctg
    21241 acgggcgaag acccaccgca gcgcattatg ctgcgtacag aacacgataa cgaagaaatg
    21301 tcagtatcga tgcataagca gcgcagtcag cagcagcctg gcgtcaccgc atggcgtgcg
    21361 gcgattgatc tctccagcgg acaaccccgg cggcgttaca gtttcaaact gctgtggcac
    21421 gatcgccagc gttggtttac accgcagggc ttcagccgaa tgccgccggc acgactggag
    21481 cagtttgccg tcgatgtacc ggatatcggc ccacaatggg ctgcggatca gattttttat
    21541 cagatcttcc ctgatcgttt tgcgcgtagt cttcctcgtg aagctgaaca ggatcatgtc
    21601 tattaccatc atgcagccgg acaagagatc atcttgcgtg actgggatga accggtcacg
    21661 gcgcaggcgg gcggatcaac gttctatggc ggcgatctgg acgggataag cgaaaaactg
    21721 ccgtatctga aaaagcttgg cgtgacggcg ctgtatctca atccggtgtt taaagctccc
    21781 agcgtacata aatacgatac cgaggattat cgccatgtcg atccgcagtt tggcggtgat
    21841 ggggcgttgc tgcgcttacg ccacaatacg cagcagctgg gaatgcggct ggtgctggat
    21901 ggcgtgttta accacagtgg cgattcccat gcctggtttg acaggcacaa tcgtggcacg
    21961 gggggagctt gtcacaaccc tgaatcgccc tggcgcgact ggtactcgtt tagtgatgat
    22021 ggcacggcgc tcgactggct tggctatgcc agcttgccga agctggatta tcagtcggaa
    22081 agtctggtga atgaaattta tcgcggggaa gacagtattg tccgccattg gctgaaagcg
    22141 ccgtggagta tggacggctg gcggctggat gtggtgcata tgctggggga ggcgggtggg
    22201 gcgcgcaata atatgcagca cgttgctggg atcaccgaag cggcgaaaga aacccagccg
    22261 gaagcgtata ttgtcggcga acattttggc gatgcacggc aatggttaca ggccgatgtg
    22321 gaagatgccg ccatgaacta tcgtggcttc acattcccgt tgtggggatt tcttgccaat
    22381 accgatatct cttacgatcc gcagcaaatt gatgcccaaa cctgtatggc ctggatggat
    22441 aattaccgcg cagggctttc tcatcaacaa caattacgta tgtttaatca gctcgacagc
    22501 cacgatactg cgcgatttaa aacgctgctc ggtcgggata ttgcgcgcct gccgctggcg
    22561 gtggtctggc tgttcacctg gcctggtgta ccgtgcattt attacggtga tgaagtagga
    22621 ctggatggca aaaacgatcc gttttgccgt aaaccgttcc cctggcaggt ggaaaagcag
    22681 gatacggcgt tattcgcgct gtaccagcga atgattgcgc tgcgtaagaa aagtcaggcg
    22741 ctgcgtcgtg gcggctgtca ggtgctgtat gcggaagata acgtagtggt atttgtccgc
    22801 gtgctgaatc agcaacgtgt actggtggca atcaaccgtg gcgaggcctg tgaagtggtg
    22861 ctccccgcgt caccgtttct taatgtcgcg caatggcaac gcaaagaagg ccatgggcaa
    22921 ctgacaaacg ggattctggc tttgcctgcc atttcggcta cggtatggat gaactaacgt
    22981 tttataacgc cttgtgtgac gcctgcgcca tcatccgcgg ataaaactgc cagaagcggg
    23041 tttcgagggc gtcataatga gcgtctaaat cgtaccagga gtcacgcagg gcatccagac
    23101 gtgggcggcg gcttgccatg ccgtttaaca cgttctggat gaaatccata tcgcgatagc
    23161 gcaccagcca ctgctctgac cacaagtaat tgttcagatt gataaaacgt ggcggtgagt
    23221 ccggcaaaat cgtcatcact tgctcgcggg cataacagac aaattcctgt agcggaaagt
    23281 ccggcgacag ctgcgaccag tggcgggaaa gaaagtgatc ccacatgaca tccagcgtaa
    23341 taggcgcaac gcggcgagtt tcactacgaa accattcccg tgcttcgcgg acttccggca
    23401 gattgtcggt taatacgtcg atacgtcgat gcatatgaat gccagccacg acgtcgggcg
    23461 gaaaactttc ttcaggattt ccgcgtacga aatcagccag taaattgccg gaaagcgagc
    23521 tttccgcgag atgggctaaa tgcaggtgag ctaaaaaatt catcgattat attctatcca
    23581 aaagggggta aaggttgcag ggagagcgcc ccggcactag actacccgcc tcttatttta
    23641 gtctgagtca gtgtcatgcg cgttaccgat ttctcctttg aattgcccga atccctgatt
    23701 gcccactatc ccatgcctga acgcagtagc tgtcgtttac tgtcgctgga cgggccgacg
    23761 ggcgcgctga cgcacggtac tttcaccgat ttacttgata agctcaaccc cggcgatctt
    23821 ctggttttta ataatacccg cgtgatcccg gcgcgcctgt ttgggcgtaa agccagcggc
    23881 ggcaagattg aagtgctggt tgaacggatg ctcgacgaca aacgcattct tgcgcatatt
    23941 cgcgcctcga aagcgccaaa acctggcgca gaactgctgc tgggcgatga cgaaagtatt
    24001 aacgcaacaa tgaccgcgcg ccacggcgca ctgtttgaag tcgaatttaa tgatgaacgc
    24061 tcggtgctgg atattctcaa cagcatcggc catatgccgc tgccgccgta tatcgaccgt
    24121 ccggacgaag acgctgaccg cgaactttat caaaccgttt atagcgaaaa accgggcgcg
    24181 gttgcagccc cgaccgcagg tctgcatttt gacgaacctt tgctggaaaa attacgcgcc
    24241 aaaggcgtgg agatggcgtt tgtgacgttg cacgttggtg cgggcacctt ccagccggtg
    24301 cgcgtcgaca ccattgaaga tcacatcatg cactcggaat acgctgaagt gccgcaggat
    24361 gtggtagacg cggtactggc ggcgaaagcg cgcggtaacc gggtgattgc ggttggcacc
    24421 acctcagtac gttcgctgga aagcgcggct caggcggcga aaaacgatct tatcgaaccg
    24481 ttctttgacg atacccaaat ctttatctat ccgggcttcc agtacaaagt ggtcgatgcg
    24541 ctggtgacaa acttccacct gccggaatcg acgctgatta tgctggtttc ggcctttgcc
    24601 ggttatcaac acaccatgaa tgcctataaa gcagcggtag aagagaaata tcgctttttt
    24661 agttacggtg atgcgatgtt tatcacgtac aatccgcagg caattaatga gcgcgtcggg
    24721 gagtaattcc gcggcgctgg tttaaaacgt tggactgttt ttctgacgta gtggagaaaa
    24781 aatgaaattt gaactggaca ccaccgacgg tcgcgcacgc cgtggccgcc tggtctttga
    24841 tcgtggcgta gtggaaacgc cttgttttat gcctgttggc acctacggca ccgtaaaagg
    24901 gatgacgccg gaagaagttg aagccactgg cgcgcaaatt atcctcggca acaccttcca
    24961 cctgtggctg cgcccgggcc aggaaatcat gaaactgcac ggcgatctgc acgattttat
    25021 gcagtggaag gggccgatcc tcaccgactc cggcggtttc caggtcttca gccttggcga
    25081 tattcgtaaa atcaccgaac agggcgtgca cttccgtaac ccgatcaacg gcgatccgat
    25141 tttcctcgat cctgaaaaat caatggagat tcagtacgat cttggttcgg atatcgtcat
    25201 gatctttgat gagtgtacgc cgtatcctgc tgactgggat tacgcaaaac gctccatgga
    25261 gatgtctctg cgttgggcga agcgtagccg tgagcgtttt gacagtctcg gaaacaaaaa
    25321 tgcgctgttt ggtatcattc agggcagcgt ttacgaagat ttacgtgata tttctgttaa
    25381 aggtctggta gatatcggtt ttgatggcta cgctgtcggc ggtctggctg tgggtgagcc
    25441 gaaagcagat atgcaccgca ttctggagca tgtatgcccg caaattccgg cagacaaacc
    25501 gcgttacctg atgggcgttg gtaaaccaga agacctggtt gaaggcgtac gtcgtggtat
    25561 cgatatgttt gactgcgtaa tgccaacccg caacgcccga aatggtcatt tgttcgtgac
    25621 cgatggcgtg gtgaaaatcc gcaatgcgaa gtataagagc gatactggcc cactcgatcc
    25681 tgagtgtgat tgctacacct gtcgcaatta ttcacgcgct tacttgcatc atcttgaccg
    25741 ttgcaacgaa atattaggcg cgcgactcaa caccattcat aaccttcgtt actaccagcg
    25801 tttgatggcg ggtttacgca aggctattga agagggtaaa ttagagagct tcgtaactga
    25861 tttttaccag cgtcaggggc gagaagtacc acctttgaac gttgattaat attaataatg
    25921 agggaaattt aatgagcttt tttatttctg atgcggtagc ggcaacgggt gcaccggcgc
    25981 aaggtagccc gatgtctttg attttgatgc tggtggtatt cggtctgatt ttctatttca
    26041 tgatcctgcg tccacagcag aagcgcacca aagaacacaa aaagctgatg gactccattg
    26101 ccaaaggtga tgaagttctg acgaacggtg gcctggttgg tcgcgtaacc aaagtagcgg
    26161 aaaacggcta cattgctatc gcgctgaatg acaccactga agtagttatt aaacgtgact
    26221 tcgtagctgc cgtcctgccg aaaggcacca tgaaggcgct gtaattaaaa tttttcccta
    26281 agggaattgc cgtgttaaac cgttatcctt tgtggaagta cgtcatgctg atcgtggtga
    26341 ttgtcatcgg tctgctgtat gcgcttccca acctgtttgg tgaggatccg gctgttcaga
    26401 tcactggtgc gcgcggagtc gccgccagtg agcaaacgct gatccaggtc cagaaaacgt
    26461 tacaagaaga aaaaataact gctaagtctg tggcactgga agagggcgct attcttgcgc
    26521 gcttcgactc cactgacacc cagctgcgcg ctcgtgaagc attaatgggc gttatgggtg
    26581 acaaatacgt cgtggcgctt aaccttgccc cggcaacgcc gcgctggctg gcagctattc
    26641 acgctgagcc gatgaagctc ggccttgacc tgcgtggcgg cgttcacttc ctgatggaag
    26701 tggatatgga caccgcgctt ggcaaactcc aggaacaaaa tatcgatagc ctgcgcagcg
    26761 atctgcgtga aaagggcatc ccgtatacca ccgttcgtaa agaaaacaac tacggcctga
    26821 gcattacttt ccgcgatgct aaagctcgtg atgaagccat tgcgtatctg agcaagcgcc
    26881 atccggacct ggtgattagt agccagggca gcaaccagct gcgtgcggta atgagcgatg
    26941 ctcgtctgag tgaagcgcgt gaatatgcgg tgcagcagaa catcaatatc ctgcgtaacc
    27001 gtgtaaacca acttggcgtg gcggagccgg tggttcagcg tcagggtgct gaccgtatcg
    27061 ttgttgaact gccaggtatt caggacactg cgcgtgcgaa agagattctg ggtgcgacgg
    27121 caacgctgga attccgtctg gtaaacacca acgttgacca ggccgctgcg gcatccggtc
    27181 gcgtaccggg cgactctgaa gtgaaacaga cccgcgaagg tcagccagtt gtgctgtaca
    27241 aacgcgtaat tctgaccggt gaccatatca ccgactccac ttccagccag gacgaataca
    27301 accaaccgca ggttaacatc tcgctcgata gcgctggtgg taacatcatg tctaacttca
    27361 ctaaggacaa catcggcaaa ccgatggcaa ccctgtttgt ggagtacaaa gacagcggta
    27421 agaaagatgc aaatggtcgt gcggttctgg tgaaacagga agaggtgatt aacatcgcca
    27481 acatccagtc tcgtctgggt aacagcttcc gtatcaccgg catcaacaac ccgaacgaag
    27541 cccgtcagct gtcactgctg ctgcgtgccg gtgcgttgat cgcgccgatt cagattgttg
    27601 aagaacgtac cattggccca accctgggta tgcagaacat tgaacagggg ctggaggctt
    27661 gccttgccgg tctgctggtt tctattctgt tcatgatcat cttctataag aagtttggtc
    27721 tgattgcgac cagtgctctg attgccaact tgatcttaat cgtcggcatt atgtcgctgt
    27781 tgccaggcgc aacgctgagt atgccaggta tcgcgggtat cgtcttaacc cttgcggtgg
    27841 cggtcgatgc gaacgtactg atcaacgaac gtattaaaga agagttgagc aacggacgta
    27901 ctgttcaaca ggcaattgat gaaggttatc gtggcgcatt cagttctatc ttcgatgcga
    27961 acatcaccac gctgattaaa gtcatcatcc tgtacgcagt gggtaccggg gcaattaaag
    28021 ggttcgcgat tactaccggt atcggtgtgg cgacgtcgat gtttaccgcg attgtcggta
    28081 cgcgtgccat cgtaaacctg ctatatggcg gcaagcgcgt caagaagctg tcaatctgag
    28141 gagtgcgatg tggcacagga atatactgtt gaacaactaa accacggccg taaagtctat
    28201 gactttatgc gctgggacta ctgggctttc ggcatctctg gtctgctgtt aatcgccgct
    28261 atcgttatta tgggcgtgcg cggctttaac tgggggctgg atttcaccgg tggtacggtt
    28321 atcgaaatta cgctcgaaaa accggctgaa attgacgtaa tgcgtgatgc attgcaaaaa
    28381 gccggttttg aagagccaat gctgcaaaac tttggtagca gccacgacat catggtccgt
    28441 atgccgcctg ctgaaggcga aaccggcggt caggtgctgg gcagccaggt tctgaaggtg
    28501 attaacgaat ccaccaatca gaatgcagca gtgaagcgta ttgagttcgt cggtccgagc
    28561 gtgggggcag accttgcgca aaccggtgcg atggcgttga tggcagcgct gctgtctatc
    28621 ctcgtgtacg taggtttccg ctttgagtgg cgactggcgg caggggtggt tattgcgctg
    28681 gcgcacgacg ttatcattac gctgggtatt ttgtcgttat tccatatcga gattgacctg
    28741 accattgtgg catcgttgat gtcggttatc ggttactcgc ttaacgacag tatcgtggta
    28801 tcggaccgta ttcgtgaaaa cttccgcaag atccgtcgcg gtacgcctta cgaaatcttt
    28861 aacgtgtcct tgacccagac gctgcaccgt accttgatca catccggtac taccttgatg
    28921 gttatcctga tgctgtacct cttcggtggt ccggtactgg aaggcttctc gctgaccatg
    28981 cttatcggtg tttccatcgg tactgcatct tccatctatg tggcatctgc gttggctctg
    29041 aaactgggta tgaagcgcga acacatgttg cagcagaaag tggaaaaaga aggggcggat
    29101 cagccgtcaa ttctgcctta atcaagttcc cgttgatgtt gaaaatcccg gtcagaagat
    29161 cgggattttt tttgatgtat ggagattgcg aaaatccccg catcttggga aactgcgcgt
    29221 aaccctacat ttcatccagg taacttttca tggctatcat cccaaaaaac tatgcgcggt
    29281 tagaaagcgg ctatcgcgaa aaagcattaa aaatctatcc gtgggtctgc ggtcgctgtt
    29341 cccgcgagtt tgtctattcc aacctgcgtg aacttactgt tcaccacatt gatcacgacc
    29401 ataccaataa cccggaagat ggcagtaact gggaattgct ttgtctctat tgccacgatc
    29461 atgagcattc gaaatatacc gaagcggatc agtatggtac gaccgttatc gcaggggaag
    29521 atgcgcagaa agatgtcggt gaagcgaagt acaacccatt cgctgacctg aaagcgatga
    29581 tgaacaagaa gaagtgatta aaacgtaaaa ttgcctgatg cgctacgctt atcaggacta
    29641 cgttatttca gcaatatatt gaatttacgt gcttttgtgg gtcggacaaa gcgtttacgc
    29701 cgcatccggc atgaacaaag cgcacgttgt taacaatctg aaatgccggg aataaatccc
    29761 ggcattttca taatcagaag ttgtaaccta caaccaggta accaccccag ccggtagagc
    29821 gaacgttgaa gttgccgttg ccgaagttca gttctgcatc gtcgttccac tgaccaccgt
    29881 cgtgccagta acgagctacg acagagtagt gccagtgatc gtagttcaga gccagaatat
    29941 ggctggaagc gatagagtta ttagtacggg tcttaatacc gttgattgcg ttaccgctgt
    30001 catcccctaa atcggaaccc cagtcgaagt tggtgaagcc gatgtagctc agctgaccgc
    30061 cccacagatc ggtaatcggc acaaagtatt taattttgaa acggtaaccg tcccactcgt
    30121 tttcgttcgc tgcgccatag ttctgccact ggtatttcgc atagacgttc atggacaggc
    30181 tcatcggcag accagtatcg atgtcggtac ccagacccat ataccaggtg ctctggcgac
    30241 catctttatt acgacccatg tcgtaaatgt agttgttcgc gaagtaccac tctttgaacg
    30301 gaccgaagct aaggtcagta ttggtcagct tgtcgatgga gaaacgtggt tcgatttcca
    30361 taaacagcgg agagccgtgg ttccagatac ctttggcatc ggagttaccg ccgaagaata
    30421 ccggcgcatc cgcataacca tagaagtcga accagtcttt tttagcgaat gcttcgtact
    30481 caaggtaggt atcgttgcgg atctgcggtc caaaacgggt gtgatagctt ccgacaacgt
    30541 taacgctctg gtgccaccag tcggaaagat actgcggttt gtcgttttca gctgcgttga
    30601 cagtaaaaga cgaagagagc gccagtaccg caccggctgc cagtaatgtt tttttcatat
    30661 gtatgccact gtttgaaaat cccttgcggg agtgaaaaag gcgcaaattg cgtttctaaa
    30721 tatttcgtat acagaggagc ctattataaa aatcattgct cacaaaaata tgtttcgttt
    30781 cacagttcta tcatttacgt aatcgattgc gttcacgttt acacacattc gggcggggat
    30841 tgtactgact ttcgcccttg ttgcaaactt ttactatcaa tccaggtgat gtaagcagag
    30901 taacaaaatg acggggaaat atgttgcggg acgcacaaaa tcgtcccgaa atacgtttac
    30961 tgcataacag gttgaatgtc gtggatacgt acaaagccta actgatccac agaaatcccc
    31021 tttaattgta acggaatatc aacatcgctg ggggcgagga cgctggcagg ggcattaatc
    31081 aattgattct gcacattgat ttcctgaaag ttgtctattg ttccctgaat ttgcccatat
    31141 tcaacagttc cgctgaatgc gggcaaggga tcattcgact cgccctgaat acgtagcgtc
    31201 agggtggtgc catctgcatc aggcgtaata ttcaccagcg acatacgtaa agtaccgatc
    31261 tggctttcca gtcttgccgg tgttttcgct ccaggcagaa ggtaaacccc actgctggat
    31321 tttgcattca gcctgttttg ctgagtgatt ttgaccgttt ctttattgag ttgcgtcatc
    31381 tcctggttca gcgtgctgac gctgtgtttc atctgtcgca cttcgctctg ctgcacacag
    31441 gcgctaaggc tgaacaggct tcccagcagg agcagtcgaa aaacgttagt attcataggt
    31501 cgtccctttc tcgtcgcaat tagcctaatg gtagagggta aaagcagcga aagcatcgat
    31561 cctttatctc aaaagcgttg cgcctttgtt gtatcgtcag ttcagggtaa aatagatttc
    31621 tgttaaccac ctggtcagga cgccgtatgc attgcccatt ctgtttcgcc gtggacacta
    31681 aggtaattga ctctcgtctc gtgggcgagg gttcatccgt acgccgccgt cggcagtgtc
    31741 tggtgtgtaa tgaacgtttc accacctttg aagtggcgga gctggttatg ccgcgtgttg
    31801 taaaaagcaa cgacgtgcgt gaaccgttta atgaagagaa attgcgtagc ggaatgctgc
    31861 gggcgctgga aaaacgtccg gtgagttccg atgacgtcga aatggcaatc aatcatatta
    31921 aatcgcagct gcgcgccacc ggtgagcgcg aagtaccgag caagatgatt ggcaatctgg
    31981 tgatggagca attgaaaaag ctcgataaag tcgcctatat ccgttttgcc tctgtctacc
    32041 gcagtttcga agatatcaaa gaatttggcg aagagatcgc gcgcctggag gactaagccg
    32101 tgcaggacga gtattacatg gcgcgggcgc taaagctggc gcaacgagga cgttttacca
    32161 cgcatcccaa cccgaatgtc gggtgcgtca ttgtcaaaga tggcgaaatt gtcggtgaag
    32221 gttaccacca acgtgcgggt gaaccacatg ccgaagtaca cgcgttgcgt atggcgggtg
    32281 aaaaagccaa aggtgcgacc gcctatgtca cactcgaacc ctgtagccat catggtcgta
    32341 cgccaccgtg ctgtgacgca ctcatcgccg ctggcgtagc gcgcgtggtt gcctcgatgc
    32401 aagatcctaa cccgcaggtc gctgggcgtg gactttaccg tctgcaacag gctggcattg
    32461 acgtcagcca cgggctgatg atgagtgaag ccgagcaatt gaataaaggc tttctcaagc
    32521 ggatgcgcac cggctttcct tatattcagt taaaacttgg cgcatcgctt gatggccgca
    32581 cggcgatggc gagcggcgaa agccagtgga tcacttcgcc tcaggcgcgg cgcgacgtac
    32641 aacgacagcg cgcacaaagt catgccattt taaccagcag cgccacggtg ctggcggatg
    32701 atccagcctt aacagtgcgt tggtctgaac tggatgaaca aactcaggcg ctctacccgc
    32761 aacaaaatct ccgccagcca gtacgtattg tgattgatag ccaaaatcgc gtgacgccgg
    32821 aacatcgcat tgtgcagcag cccggcgaaa cctggttcgc gcgtacgcag gaagattcta
    32881 gtgagtggcc ggaaacggtg cgtaccttgc tgattccaga gcataaaggt catctggatc
    32941 tggttgtact gatgatgcaa ctgggtaaac agcaaattaa cagcatctgg gtggaagcgg
    33001 ggccaacgct cgctggcgca ttgctgcagg cgggtttagt cgatgagctg attgtctata
    33061 tcgcacctaa actattaggc agcgacgccc gcggattatg cacgctgcca gggcttgaga
    33121 aattagccga cgccccccaa tttaaattca aagagatacg tcatgtaggc ccggatgttt
    33181 gcctgcattt agtgggtgca tgatctcccg gctcgaaagg gaagcagcgc acgaaatatt
    33241 atgctaaaat ccgcccccct gcggggccat actcgaaccc gaaggaagaa aatgaacatt
    33301 attgaagcta acgttgctac cccggacgct cgcgtcgcca tcaccatcgc gcgtttcaac
    33361 aactttatca atgacagcct gctggaaggt gcaattgacg cactgaaacg tatcggtcag
    33421 gtaaaagatg aaaacattac cgttgtttgg gtgcctggtg cctatgagct gccgctggcg
    33481 gcgggtgcac tggctaaaac cggtaaatac gacgcggtga ttgcgctggg tacggttatt
    33541 cgtggtggca ctgcccactt tgaatatgtc gctggtggtg caagcaacgg cctggcgcat
    33601 gttgcccagg acagcgaaat tccggttgct tttggggttc tgaccactga aagcattgaa
    33661 caagcgatcg aacgtgctgg caccaaagct ggcaacaaag gtgcagaagc tgcactgacc
    33721 gcgcttgaaa tgattaatgt attgaaagcc atcaaggcct gaaattagta aggggaaatc
    33781 cgtgaaacct gctgctcgtc gccgcgctcg tgagtgtgcc gtccaggcgc tctactcctg
    33841 gcagttgtcc cagaacgaca tcgctgatgt tgaataccag ttcctggctg aacaggatgt
    33901 aaaagacgtt gacgtcctgt acttccgtga gctgctggcc ggggtggcga ctaacaccgc
    33961 atacctcgac ggactgatga agccatacct gtcccgcctg ctggaagaac tgggacaggt
    34021 agaaaaagca gtactgcgca ttgcgctgta cgagctgtct aaacgtagcg atgtgccata
    34081 caaagtggcc attaacgaag cgatcgaact ggcgaaatcg ttcggcgcag aagacagcca
    34141 taagttcgtc aacggcgtac tcgataaagc agcacctgtg attcgcccta acaaaaagtg
    34201 atatccaggc cggtagattc acggaagacc gttccgcgat cgccggcctt ttctttttta
    34261 cctgctgagg cataacgtat ggcatgtggc gagttctccc tgattgcccg ttattttgac
    34321 cgtgtaagaa gttctcgtct tgatgtcgaa ctgggcatcg gcgacgattg cgctcttctc
    34381 aatatccccg agaaacagac cctggcgatc agcactgata cgctggtggc gggcaaccac
    34441 ttcctccatg atatcgatcc tgctgatctg gcgtataaag cactggcggt gaacctaagc
    34501 gatctggcag cgatgggggc cgatccggcc tggctgacgc tggcattaac cttaccggac
    34561 gtagacgaag cgtggcttga gtccttcagc gacagtttgt ttgatcttct caattattac
    34621 gatatgcaac tcattggcgg cgataccacg cgtgggccat tatcaatgac gttgggtatc
    34681 cacggctttg ttccgatggg acgagcctta acgcgctctg gagcgaaacc gggtgactgg
    34741 atctatgtga ccggtacacc gggcgatagc gccgccgggc tggcgatttt gcaaaaccgt
    34801 ttgcaggttg ccgatgctaa agatgcggac tacttgatca aacgtcatct ccgtccatcg
    34861 ccgcgtattt tacagggaca ggcactgcgc gatctggcaa attcagctat cgatctctct
    34921 gacggtctga tttccgatct cgggcatatc gtgaaagcca gcgactgcgg cgcacgtatt
    34981 gacctggcat tgctgccgtt ttctgatgcg ctttctcgcc atgttgaacc ggaacaggcg
    35041 ctgcgctggg cgctctctgg cggtgaagat tacgagttgt gtttcactgt gccggaactg
    35101 aaccgtggcg cgctggatgt ggctctcgga cacctgggcg taccgtttac ctgtatcggg
    35161 caaatgaccg ccgatatcga agggctttgt tttattcgtg acggcgaacc tgtcacgttt
    35221 gactggaaag gatatgacca ttttgccacg ccataaagat gtcgcgaaaa gtcgcctgaa
    35281 gatgagtaat ccgtggcatc tgcttgctgt cggattcgga agtggattaa gcccaatcgt
    35341 tcctggcacg atgggatcgc tggcagcgat tccgttctgg tatctgatga cctttttacc
    35401 ctggcagctc tactcgctgg tggtgatgtt ggggatctgt atcggcgtct atctttgtca
    35461 tcaaacggcg aaagatatgg gcgtgcacga tcacggcagc attgtctggg acgaatttat
    35521 tggtatgtgg atcacgctca tggcgctgcc gaccaatgac tggcagtggg ttgccgccgg
    35581 gtttgtgatt ttccgtattc tggatatgtg gaagccgtgg ccgatccgct ggtttgatcg
    35641 caatgtacat ggcggcatgg ggatcatgat cgatgatatt gtcgccgggg tgatctccgc
    35701 aggtatcctg tactttatcg gtcatcactg gccgctgggt attctgtcgt agttgtgcac
    35761 cagtgcctga tgtgacgctt gttgcgtctc atcaggcctg gactcttatt taaatcctac
    35821 gaccgcatgc ggtttatacg gcgtttccag ttcggcaatc tgttctggct tcaaagtgat
    35881 atccaccgcg ttcagcagct catcaagctg ttcttcccgc gatgtaccga taatcggtgc
    35941 ggcaatgccc ggtttactca acaaccaggc cagcgcaact tgtgctcgtg ttgcaccgag
    36001 ttcttcactg acgcccgtta accgctctgc gatctgcgcg tcattttcat cgctttcttt
    36061 atagagattt ctccccacct catcagacac cagtcgtgcg gtagtttctc cccacggacg
    36121 cgtcagtcgc ccccgcgcca gcgggctcca cggaatcacc gccacgccct cctgataaca
    36181 cagaggcagc atctcacgct cttcttcgcg ataaatcaga ttgtagtgat cctgcatact
    36241 gataaactgc gcccagccgt gctgtttttg tagcttcagg gcctgagcaa actgcgaagc
    36301 gtgcatggat gacgcgccga tataacgcgc tttcccggct tttaccacgt cgttcagggc
    36361 ttccagcgtc tcttcgatcg gcgtgttgta atcccagcga tgaatttgca ggatatcgac
    36421 ataatccatg ccgagacggc gcaggctgtc gtcgatagag cgcaaaattt gtgcacggga
    36481 taatccttcc ggtaaatcac caacgcgatg gaacactttc gtcgcaacga ccacgtcttc
    36541 acgacgggcg aaatcccgca gtgcgcgacc aacgatctct tcgctgctgc catcggaata
    36601 gctattggcg gtatcaaaga aatttatgcc gccttccagt gcacgtttaa ttatgggacg
    36661 gctgctttct tccggcagtg tccatgcgtg attaccgcga tctggctcgc caaaggtcat
    36721 acagccgagg caaagtcggg aaacgcgaag gtcggttttt cctaaggggt tgtattgcat
    36781 gctgccactc ccgctatact cgtcatactt caagttgcat gtgctgcggc tgcattcgtt
    36841 caccccagtc acttacttat gtaagctcct ggggcttcac tcgtttgccg ccttcctgca
    36901 actcgaatta tttagagtct atgaataatt tcttaagcat agcaggagtg gagtagggat
    36961 tatgccagcc aggccttgat tttggcttcc ataccggcgg catcgaggcc gagttcggcg
    37021 cgcatttctt cctgagttcc ttgtggaata aagaagtcag gcaggccaat gttcagcacg
    37081 ggtactggtt tacgatgggc cattagcact tcgttcacgc cgctgcctgc gccgcccata
    37141 atggcgtttt cttctacggt gaccagcgct tcatggctgg cggccatttc cagaattaac
    37201 gcttcatcaa gcggtttcac aaaacgcata tcgaccagcg tagcgttcag cgattcagcg
    37261 actttcgccg cgtctggcat cagcgtacca aagttaagga tcgccagttt ctcgccacga
    37321 cgcttcacaa tgcctttgcc aattggcagt ttttccagcg gcgtcagttc cacgccaacc
    37381 gcgttaccgc gcgggtagcg caccgcggac gggccgtcgt tatagtgata gccggtatag
    37441 agcatctggc gacattcgtt ttcatcgctc ggggtcataa tgaccatttc cggtatacag
    37501 cgcaggtaag agaggtcaaa agcgccctga tgggtttgac cgtcagcacc aacaatgccc
    37561 gcgcggtcga tggcgaacag gaccgggagc ttttgaatcg ccacgtcatg cagcacctga
    37621 tcataggcgc gttgcaggaa agtggagtaa atcgcgacaa tgggtttgta cccaccaatc
    37681 gccagaccgg cggcaaaggt caccgcgtgt tgctcggcga ttgccacgtc gaagtaacga
    37741 tccgggaatt tacgtgaaaa ctcgaccatg ccggaacctt cacgcatcgc cggagtaatc
    37801 gccatcagct tgttgtcttt cgctgccgtt tcgcacaacc agtcgccaaa gatttttgaa
    37861 tagctcggca aaccgccgct acttttcggc aaacaaccgc tggagggatc aaatttaggc
    37921 acggcgtgga aagtgatggg gtctttttct gccggttcat aaccacgacc ttttttggtc
    37981 atgatatgca ggaactgcgg gcctttcagg tcgcgcatgt tcttcagcgt ggtgataagc
    38041 cccagcacat cgtgaccgtc aaccgggccg atgtagttaa agcccagctc ttcaaacaac
    38101 gtgccaggca ctaccatgcc tttaatatgt tcttcggtac gtttgagcag ctctttaatt
    38161 ggcggaacgc cagagaaaac ttttttcccg ccttcgcgca gcgaagagta aagcttaccg
    38221 gaaagcagct gtgccagatg gttattgagc gcgccgacat tttcggaaat cgacatttca
    38281 ttgtcgttga ggaccaccag catatcagga cggatatcgc ccgcgtgatt catcgcttca
    38341 aacgccatgc cagcggtaat cgcgccatcg ccaatgacac agacggtgcg gcgatttttg
    38401 ccttctttct cggcagcaac cgcaatacca attccggcac tgatggaggt tgatgaatgc
    38461 ccgacgctta atacgtcata ttcgctttcg ccgcgccacg ggaacgggtg caggccgcct
    38521 ttctgacgga tggtgccgat tttgtcgcgg cgtccggtca aaattttatg cggataagcc
    38581 tgatgcccca catcccaaat caattggtca aacggggtgt tgtagacata gtgcagcgcc
    38641 acggtcagtt cgaccgtgcc cagcccggag gcgaagtgcc cgctggaacg gctcacgctg
    38701 tcgagtaaat agcggcgcag ttcgtcgcag agtttcggta aactctcttt cggcaacagt
    38761 cgtaactcct gggtggagtc gaccagtgcc agggtcgggt atttggcaat atcaaaactc
    38821 atcaggggcc tattaatact tattgtttat ttattacgct ggatgatgta gtccgctagc
    38881 gcttccagtg ccgaggtatc gagtgattgt tcagccagtt gtttcagcga ctggcgggca
    38941 tcgtcgatca gatcccgggc tttcttccgg gcttgctcaa gacccagaag tgcagggtag
    39001 gtacttttac caagttgctg gtcggcaccc tggcgttttc ccaacgttgc agtatctcct
    39061 accacatcca ggatgtcatc ctgaacctgg aaggcaaggc cgatgctctc tgcgtacttg
    39121 tcgagtactg gcagagcacg acgcccttta tctccggcgc ttaatgcacc aaggcgaacg
    39181 gcggcgcgaa tcaatgcgcc ggttttatga cgatgaatac gctcaagcgc gtccagaggt
    39241 acgtgtttgc cttccgcgtc taaatctaat gcctgaccac cgcacattcc ggcaataccg
    39301 ctggcgctcg ccagttcaga aatcatcgaa attctgtcgc gatccgacac ttccggcata
    39361 ttggcatcgc ttagaatcga gaacgccagc gtttgtaaag cgtcgccagc gagaatcgcg
    39421 tttgcttcgc caaacttcac atggcaggtc ggcaaaccgc gacgcagatc gtcatcatcc
    39481 atcgccggta aatcatcatg aattaatgag taagcgtgga tacactctac ggcggcagcg
    39541 ggtgcgtcca gcgtgtttgt gctaacgcca aacatatgac cggtggcata aaccaggaag
    39601 ggtcgcagac gcttaccacc taataatgcg ccatactgca tggtttcgac cacgggagtg
    39661 ttctgaaagg gcagtggggc gataaaacgg ctcagcgcct ggttggcctg cttaacgcag
    39721 gcttcgagtt gctgcggaaa gtccattact cattgtccgg tgtaaaaggg gttagagagg
    39781 cgtcttcatt gtcagacagc agaatttgta cgcgctgttc ggcttgttgt aatttggcct
    39841 gcccctgacg tgccagctgc acgccgcgtt cgaactcgtt cagcgcctct tccagcggca
    39901 ggtcgccact ttccagacgg gttacaatct gttccagctc gctcagcgcc ttttcaaagc
    39961 tggcgggagc ctcatttttc ttcggcataa tgaatgtctg actctcaata tttttcgccc
    40021 cgtcatggta acggactcag ggcaaatagc aaataacgcg caatggtaag gtgatgtgcg
    40081 cagcaaagcg atgttagtgg tatacttccg cgcctggatg cagccgcagg tgtgggctgc
    40141 tgtatttttc cctatacaag tcgcttaagg cttgccaacg aaccattgcc gccatgaagt
    40201 ttatcattaa attgttcccg gaaatcacca tcaaaagcca atctgtgcgc ttgcgcttta
    40261 taaaaatcct taccgggaac attcgtaacg ttttaaagca ctatgatgag acgctcgctg
    40321 tcgtccgcca ctgggataac atcgaagttc gcgcaaaaga tgaaaaccag cgtctggcta
    40381 ttcgcgacgc cctgacccgt attccgggta tccaccatat tctcgaagtc gaagacgtgc
    40441 cgtttaccga catgcacgat atttttgaga aagcgttggt tcagtatcgc gatcagctgg
    40501 aaggcaaaac cttctgcgta cgcgtgaagc gccgtggcaa acatgatttt agctcgattg
    40561 atgtggaacg ttacgtcggc ggcggtttaa atcagcatat tgagtccgcg cgcgtgaagc
    40621 tgaccaatcc ggaagtgact gtccatctgg aagtggaaga cgatcgtctc ctgctgatta
    40681 aaggccgcta cgaaggtatt ggcggtttcc cgatcggcac ccaggaagat gtgctgtcgc
    40741 tcatttccgg tggtttcgac tccggtgttt ccagttatat gttgatgcgt cgcggctgcc
    40801 gcgtgcatta ctgtttcttt aaccttggcg gcgcggcgca tgaaattggc gtgcgtcagg
    40861 tggcgcatta tctgtggaac cgttttggca gctcccaccg cgtgcgtttt gtcgctatta
    40921 atttcgaacc ggtcgtcggg gaaattctcg agaaaatcga cgacggtcag atgggcgtta
    40981 tcctcaaacg tatgatggtg cgtgccgcgt ctaaagtggc tgaacgttac ggcgtacagg
    41041 cgctggtcac cggcgaagcg ctcggccagg tgtccagtca gacgctgacc aacctgcgcc
    41101 tgattgataa cgtctccgat acgctgatcc tgcgtccgct gatttcttac gacaaagagc
    41161 acatcatcaa cctggctcgc cagattggca ccgaagactt tgctcgcacg atgccggaat
    41221 attgcggtgt tatctccaaa agcccgacgg tgaaagcggt taagtcgaag attgaagcgg
    41281 aagaagagaa attcgacttc agcattctcg ataaagtggt tgaagaagcg aataacgttg
    41341 atatccgcga aatcgcccag cagaccgagc aggaagtggt ggaagtggaa accgtcaatg
    41401 gcttcggccc gaacgacgtg atcctcgata tccgttctat cgatgaacag gaagataagc
    41461 cactgaaagt cgaagggatt gatgtagttt ctctgccgtt ctataaactg agcaccaagt
    41521 ttggcgatct cgaccagaac agaacctggc tactgtggtg tgagcgcggg gtgatgagcc
    41581 gtctgcaggc gctctatctg cgcgagcagg gctttaacaa tgtgaaggta tatcgcccgt
    41641 aatttgtggt tgtaacgtcg catctggtca gatgcgacgc ttaccgcatc cgacactact
    41701 cgtaataatt ataaatccct gccgccatca ccagttgtga tgccacttca tgggctttct
    41761 cgcgcccaac cagcaggtcg ataattttca gaccaaagtc gatagctgtc cccggcccct
    41821 ggctggtcag caattttacc cgtgcatccc agacgacgcg cttgtcctgc cattgttcag
    41881 cgggaatttt gtctttcagc gtcgggaagc cggtcatatt accaatcggg aagatatcgt
    41941 gcggcaccag cacggtggct ggcgcggcgc aaatagccgc gacgatacgc ccggaacggt
    42001 ggaactgttt aacggtttca accagcaggg tactatcgcg aaaacactct gcgcctttaa
    42061 tgccaccagg cagtacgatc acgtcatatt cgccatcagc cacctcgacc agcggcgcat
    42121 ccgccagcag cttcacgccg cgcgagcagg taatggccag gttgccatcg ctggcgacgc
    42181 tggcagtggt gactttgata ccgccgcgaa ccagcagatc gatagtggtg acggcttcag
    42241 tctcttcact accaggggcg aggcaaacca gtgccgatgc gctcatattc actctccttt
    42301 ctttttacca tttcaaacag gcgggtgttt tccggtacgg caatcccatg cgcgcgggcg
    42361 cggcgtaaga gaaaaccatt gatatagtcg atttcggtgt ggcgcagcgc gcggatatcc
    42421 tgcaacatcg acgagatatt ttccgctgtg gcatcaatca cctgcatcac gtaatcacgc
    42481 aaatcttctg ctgaagtatg atgcccttcg cgctcgatca ctgccgcgac ttcttcgcat
    42541 atctgcataa tttcttgcgg atgatgacgt aattcaccat tcgggcaatt ccagatggcg
    42601 gtcagtggat taatcacaca gttgacggcc agcttgcgcc acagctcggc gcgaatattg
    42661 ttatgccagg caacgtcagg caacacggtt tgcaaaatat ccgccagata actgtaatcg
    42721 ccatcctgtt gccgtgccgg accaatatgc gtgattccgt ttgccacatg aataatgaca
    42781 ttgccgtcgc ggcgggcggc atgggtggtg gtgcccatca gtaatggctg ctgaatgttt
    42841 tgcaattctt cgatggtgcc catgccgttg tgaattaaca gtattggcgt agctacaggc
    42901 agtgtggatg cgaggctttt gacggcatcg gaaacctgcc atgctttcag cgtcaccagg
    42961 agcagatcgc tggtggcgag aaaatcggga tcgttggcgg tcagcgattc gttaaatatc
    43021 gcaccatctg tctcaaccag attcacacta caataaggtt gcggtacgcg cagccagccc
    43081 tgaacttcat gaccctgttt acaaagtgct gtaagccata attgccctaa ggcaccgcat
    43141 cccaatacgg taattttcat tgttcctcct cacccgcaac cactccgggt gttcaataag
    43201 gctatccctt aattgtgcat gctgttgcga ctatgcacaa ttaagggata cgtcctggtg
    43261 caggactgtt ggttatttaa ctttgcaggt attatgcttc gcatcaaaaa tgaagggaga
    43321 ggaaaagatg ccatctttcg atattgtctc tgaagttgat cttcaggaag cacgtaacgc
    43381 ggtcgataac gcgagtcgcg aagtggagtc ccgttttgac ttccgtaacg ttgaagcctc
    43441 atttgagctg aacgacgcca gcaaaaccat caaagtgttg agcgagtccg acttccaggt
    43501 caatcagttg ctggatattc tgcgtgccaa gctgctgaag cgcggcattg aaggcagttc
    43561 actggatgta ccggaaaata tcgttcatag cggtaaaacc tggtttgtgg aagcgaaact
    43621 gaaacagggc attgagagcg cgacccagaa gaaaatcgtc aagatgatta aagacagcaa
    43681 actgaaagtg caggcgcaaa ttcagggcga tgagatccgt gtgactggca aatctcgtga
    43741 tgatttgcag gctgtcatgg cgatggtacg tggtggcgat ctcggccagc cgttccagtt
    43801 caaaaacttc cgcgattaat cgcgacaatg tgcgttttgt tcatgtcaga tgcggcatga
    43861 acgcctgatt cggcctacaa cggtttgtaa ctcaagaact ttgtaggcct ggtaagcgta
    43921 gcgcagcagg caattcgccg cgttttttat gcctgacgaa ttgcctgttc aacctcaaag
    43981 cgatttgtca ctttgctgtc gattttcaca taagctgaat gctcttcttc tgcaatcaac
    44041 acttctttga cgccttcagt ttccagcaaa cgcactttta acgcttcgtt tgcggcaatg
    44101 tccgccggga tttcaatgcg caaactgctg acatacggcg gttctttcat ggtactggcg
    44161 actgccagcc acactgcggc cagcattgcg ccagcgagaa ataccccctg accgtcaaac
    44221 atgccgtcaa tccagccgcc cagcgaaccg ccaatcgcca cgccaagaaa ctggctggtg
    44281 gagtagacac ccatcgccgt acctttgtaa cctgctggcg actctttact gataagtgag
    44341 ggcagaaggg cttccatcaa attaaacgcc acaaagaaaa gctgcacgcc gaccaccagt
    44401 tgccagaact gcgtttgtgc gttccacaac acaatttccg caaccacgat caacccgacg
    44461 cagaagacaa agacttgctt cattttgcgc ttaacttcag cgtagataat gaaaggcacg
    44521 accgagccaa aggcgattaa catcgtcgcc agatagacct tccagtgttc agccgccggg
    44581 aaccccgcat cagctagctg tccgggcagg gcaacaaacg tagacatcag aagcatgtgc
    44641 agacacataa tgccaaagtt gagttttagc agccgcggtt ccgccagcac tttactgaaa
    44701 ctgcctttca ccattccgga ctcacgatta agtacgtgag tgctactgtt gggcacaacc
    44761 caaatggtca acgcaatgcc ggttgttgcc agaatagcga tcatccagaa cagcgcgtgc
    44821 agcccaagtt tgtgagtgat gatcgggcca agcaccatcg caatggcaaa ggtaatgcca
    44881 aagctcacgc cgataaacgc catcgctttg gtgcggtttt gttcgcgcgt gagatcggaa
    44941 agcagcgcca taacggcggc ggcaattgct ccagagcctt gtagcgcccg gcccagaata
    45001 attccccaga tggagtcgga aagcgcagcg ataacgctac cggcggcaaa caccgccagc
    45061 ccaccgacaa ttaatggttt gcgaccaatg cggtcggaaa gcaggccaaa cggaatctga
    45121 aaaacggcct gagtcagacc ataaatacca atggcaatac cgattaatgc ttcgctggca
    45181 ccttgcagag ccatgccgta cgtggtcaga accggcagaa ccataaacat gcccagcatg
    45241 cgcaacgaga atacggtccc taaaccccag gtcgcgcgcc tctcacctgg cgtcatttta
    45301 taatcgttca ttaccacccc ttaaaaaaat gcccacatag tgtagtgcgg agcgcatgtt
    45361 gggtaaacag agtgttaatg agaaaatatt acagaggaga gtaatttgtg tgtcaggtat
    45421 gtttttttgc attcataaaa tgaatgctct atatgttgaa aaaatgaatt ttaaaactaa
    45481 atagttttac atcaaaaaag taataatatc atttcttgat tgccatgtgc attttttatc
    45541 ttacgaatat gctagtttta tctcactaaa taaacgattt ggtgacatgg atgataatgt
    45601 ttcaggctaa atgtgtgctt attacttgga cctttatggc actgtattta ttcattaaat
    45661 ggatatttgg tggttaatta ttatggaaat atatgacagt gaggacataa aggtatacat
    45721 gttaactaaa agaatattat tttctgtaat gctgatagtt tcaccaagcg tggtggccag
    45781 cgagaaagcg catgaattgt atgacagcat atacggtggt aaacctgctc ccgatgttat
    45841 aaatacactg cataaaatgg ctgaatcggg agatatcgac gcgcagagcc tgttaggctg
    45901 ggaatattat cagcctcgtt atgataccaa acctgatgtt caggaagcga ttaaatggtt
    45961 cgagttagcg gctaaacaag gtgacagaga agctccgtta gcgctggggg gtatctacta
    46021 cgacggtgag caggtgcggg tggattatgc caaagcgtat gcactattta atcaggccgc
    46081 gcagcacggt gtgaatttag catggtccag gttgggcatt atgtacgcca atggtcagta
    46141 tgttgaggta gattgtaaga aagcgaagga atatcttgat aaaggtgtcc acatttatgg
    46201 tggcccagaa gactttctgg ctacttgtcg aaaagacatg attgacagaa aaacggttga
    46261 cgatacgtta cccgtgatta cggttacccg ttctgggatg cgagataatt ttttagataa
    46321 aggtttttcc tgcatggata gcctcttcgc caccaccaat aaattaggcg aagtggccaa
    46381 tcttcgcgtg acattcagca ttcgtcgccc atccggtaag gagattaacc aaacggttgg
    46441 cttcgcgcct ttcggtttaa accggctgaa tattagcttt actgattacc tatttggttc
    46501 atttaccagt aattcgtctc ttattcttta taaaccggaa tttgagcgaa aatcttgtgc
    46561 aactgtaagg accacaatcg ttgccgcaac ggcaaccatt aacggcaagg atgtggagtt
    46621 gctgaaagcg ggggcaattg aacaaaagtg gtaacgctgc tcagcccccg gctgaggcaa
    46681 aacttgccct ggcgggtggc gataataatc taagccgcga gaatatgctg aatcgccaaa
    46741 aacataggta cagcaccaaa agcatttggt gtcgctgaaa gctgaacaaa gccgagacga
    46801 gcataaaaat taatggcggc agggtcagca tcaagataaa cccctttaat tggtaatgcc
    46861 tggtgaatta tttttacatg ttcaaaaaaa tcacatagta ggtcctgacc aaagccccgc
    46921 ttttgatact tccgtgctac ccccaacatg actaatctga caacaccaat ttctgaaggt
    46981 tgtgaaccct gaaggacgcc agaaacgcgt tgtttttcca gcgaatatgc cgtaaaagta
    47041 cagatgccga tcagttcacc actttgtctg tcaataagtg ctttagccgc acagtcgcta
    47101 ttacgcacac ttttctttag cgatgcgcgt acaaatttat cgataacagg gttaccgcaa
    47161 tcaaactgtt tttgcccagg ataagtaata tcagactgat aattacagga gaggactatg
    47221 ggcagagtaa tctcttcatg cttgtcgacc atcgctgttt ttccttttca ttaacgcctt
    47281 gagggcgagc gtcggttgag caggttcagc gatgagttgg ttcatcagtt cccagctttg
    47341 attcgaaagc tcacggcgac gttggttatc aagcacactt tcggcgcgtt ccatagcagc
    47401 gctcaaaata aaagcgctta aatctacccc aacggcttgc gcagcttcac gaagtttgtt
    47461 tttggcgtcg ggactggttt tgagttcgac ccgcgcattt ttcagcgttt tatcgacatc
    47521 gggtagagtg gctgtttttc caggcattat gacctctttg attaactgaa tgaaatcgta
    47581 gggagtcgat ctcatacata atacggctat caaccgtatg attaatttat gttcatctgc
    47641 tcactctgtc aagacatacg gttattagcc gtatctctga tctcagacaa taaaaaaggt
    47701 gccgtagcac ctttttaata gagaggtttt gttaccacac agcagccagc agcgtatgcg
    47761 agtccggtac cataaaatca acggacatca tcaccgagag ggcagtgatg gcgatgatag
    47821 agaagccgaa cagcttgcgc gcccagattc tgtcatcagc aactttataa ccgcgcagag
    47881 ccatacctaa ccaccagacg ctaaccgccg cggcgaccac cagatattta tatccagcgt
    47941 aaccgccaag agagagcatc agcgtggcaa cggcaaaggc gatgatatac agcgtgatgt
    48001 gattcttcgc caccgaaatg ccttttacga ctggcaatac cggaatgttt gccgcctggt
    48061 aatccttaaa gcggaaaatg gcgatggcat aggagtgagg catctgccac aggctgaaga
    48121 tagccagcag gatcgctgcg ccgctatcga actcgccggt tactgcacag tagccaatca
    48181 ccggcggcgc agcgccggag agcgaaccaa tcaatgtgcc gtagactgag tggcgtttca
    48241 tgtacaggct atagacgccg acataaacca caaagcccat cacccccagc cagcaggcca
    48301 gcggattcgc gccaaaccac agcagcataa agccagcaat acccagcaag gtggcgtaca
    48361 ccagcgagac agcaggagag atcaggcctt taaccagcac ccgattcttc gtcctttcca
    48421 tctttctgtc gatatccctg tcgatgtagt tgttaaacac acaacccgac gccacaacca
    48481 gtgacacccc aaccagcgtg tagataaaca ggggataatc aatgctgccc tttgaggcca
    48541 gcaggaatcc cccaatcacc gagatcaggt tgccaaagat gatgcctggt ttcgttactt
    48601 gcaggtattg cttaaacatc ataaccgccg ctcttagtgc atcatcatgt tgtagttgag
    48661 gttccacata atccagatgg agcctacaac caggatagcg atgattagca cggtgaagac
    48721 aaacgccgtc atgttccagc cttcatctga tttggtattc atgtgcagga agcacaccag
    48781 atgcaccaga acctgtacca ctgccattgc caggattgtt cccagaatta cggccggaga
    48841 ggcagcccct gtcatcacca tccagaacgg aatcaccgtc agaatgatcg acaggataaa
    48901 gcctgtcatg taggttttta cgctgccatg ggacgcgccg ctgtgatcgg tagaatgact
    48961 cattacatcg cccccatcag ataaacaaca gtgaacacac agatccaaac cacatccagg
    49021 aagtgccaga acaggctcag gcacatgatg cgggtacggt tagtgctggt caggccgcga
    49081 cgagcgattt gtaccatcag caccgccatc cagataagac cggaagtgac gtgcagaccg
    49141 tgcgtgccga ccagcgcaaa gaacgctgac aggaagccgc tgcgatccgg acccatgccg
    49201 ttaacaatca ggtgatggaa ttcgtagatt tccatcccga taaatccggc accaaacaac
    49261 caggtcaacg ccagccagga gataacctgg cttttgttgt ttttgtacat ggcgatagcc
    49321 gccatgccgt aggtgatgga gctgaacaac agcaagaaag tttcaaccag aacgaacggc
    49381 agttcgaaaa tgtccttacc tgtcggaccg cctgcggtgc cgttcaccag aacggcatag
    49441 gtagcaaaca agattgagaa cagaatgcag tcgctcatca ggtagatcca aaatccgaag
    49501 attttggttc cgcctgcatc gtggtgcccg tgttcgtgcg cgtgggcagt cgcgtgcgtc
    49561 aaagtatcag ttgccatttt tcagccctgc cttagtaatc tcatcgaaat gctggttttc
    49621 cagtttttcg atttctgcca ccggcacgta gtaatccacg tcctcgtcga agcttttcac
    49681 gatccaggtg atgatcatgc ctgcgaagcc aacaatcgcc agccaccaga tatgccagat
    49741 catggcgaaa ccgaagatgg tggagaaagc tgcaatgacg atacctgcac cgctgttttt
    49801 cggcatatga atttcttcat agtggtcagg ctttttatac gcttcgcctt tctctttcat
    49861 ttcccagaat gcatcacgct cgtgaacgtg cggcacaacg gcaaagttat agaacggagg
    49921 cggggaagag gttgcccact ccagcgtacg gccaccccac gggtcgccag tcaggtcacg
    49981 gttctggtcg cggtcgcgaa tagaaacgta catctgaata acgaggcaga gaatacccag
    50041 cgcaatcagt actgcaccgc tagctgcaat catcagcatg gtgtggaact gcgggtcaat
    50101 ctgctggctc aaacgacggg tcatgcccat gaagcccagc gcatacagcg gcataaaggc
    50161 aacgaagaag ccgatgatcc agaaccagaa cgcgcgttta ccccaggttt cattcagttt
    50221 gaaaccgaag gctttaggcc accagtaggt catccctgcg aagcagccga agaccacgcc
    50281 gccgatgatc acgttatgga agtgcgcaat caggaacagg ctgttatgca gaacgaagtc
    50341 cgcgcccggt actgccagca gcacgccagt catcccgccc accgagaaag tgacgataaa
    50401 accgatggtc cacagcatcg cagaatggaa cacgatgcgg ccctgataca tggtgaacag
    50461 ccagttgaag atcttcaccc cggtcgggat ggcgataatc attgtggtga taccaaagaa
    50521 ggcgtttacg ttcgcgcccg cacccatcgt aaagaagtgg tgcagccaaa cgatgaacga
    50581 cagcacggtg atacagacgg ttgcccatac cagcgaggta taaccaaaca gacgtttacg
    50641 cgagaaggtt gccgcaattt cggagaacac accgaaaaca ggcaggatca ggatgtaaac
    50701 ttccgggtgg ccccaggccc aaatcaggtt gatgtacatc atcatgttgc cacccatatc
    50761 gttggtaaag aaatgggtgc ccagatagcg atccagggtc aacaacgcga cggtaaccgt
    50821 cagaattggg aaggaagcaa taatcagtac gttcgcgcac agtgatgccc aggtaaatac
    50881 tggcatcttg aacatggtca tgcccggtgc gcgcatcttc agaatggtaa cgaagaagtt
    50941 gataccggta agcgtcgtac ctataccgga tagctggaga ctccatatcc agtaatcaac
    51001 accgactccc ggactgtact ctattcccga tagcggtgga taggccagcc agccggtctg
    51061 cgcaaattcg cccacgccga gagaaacgtt aaccagaatc acaccaacaa cagtaaacca
    51121 gaagcttaag ttgttgagga acgggaacgc aacgtcacgc gcgccgatct gcagcggaac
    51181 caccaggttc atcagaccga taacgaaagg catcgccacg aagaagatca taatcacgcc
    51241 gtgcgcggtg aagatctgat cgtagtggtg aggtggcagg aagcccgctt cacccgccga
    51301 ggcaagagcc tgctggctac gcatcataat ggcgtcagca aaaccacgca gcaacatcac
    51361 aatcgccacg atgatataca tgataccgag gcgtttatgg tcgacggagg tcagccactc
    51421 tttccacagg taggtccact taccgaagta agtgatcagg ccaacgagcg ccagacctcc
    51481 caaaataatg ccagcgatcg taaccatgac gataggttca tggaacggga ctgcatcaag
    51541 tgataatttt ccgaacatct ttattcttcc tcaacccctt taatgggcgg attccgcgtg
    51601 gctcatgtcc atgccttcca taccttcgtg tgcactgtgc tcaccttctg gctgggtcat
    51661 gtccatgctc ttaccgtgag ccataaactt gttaatcaca tcggcaaaca agtctggttt
    51721 cacgttggag aaatattcca cctggttgta ttcgctaggc gcggccagtt tttcgaacgc
    51781 agccatgtca gacatggtgt tcggcgactg tttcgctttt gcgacccatt ggtcgaattc
    51841 ggcgcgatcc ggtgttgcaa tagctttgaa cttcatgcct gagaagcccg gcccgctgta
    51901 gctggcggag ataccgtcat aagtaccggg ttcgttggcg atcagatgca ggcgagtctg
    51961 cataccggcc atggcataaa tctggctacc cagacgcgga atgaagaagg agttcatcac
    52021 ggagttggag gtcactttga agtacaccgg agtgttcgcc gggaaagcga tttcattcac
    52081 ggtagcaatg ccctgttccg ggtagatgaa gaaccatttc cagtccatgg aaaccacttc
    52141 gatggtaatg ggcttctcgt cgtgtgccag cggcttgcta ggctcaagag cgtgagtggt
    52201 tttccaggtc agtaccgcaa ggaagatgat gattaagata ggcaccgtcc agaccacagc
    52261 ttccacttta ttggagtgtg accagttcgg gctgtactta gcatctttat tgctcgcacg
    52321 gtacttccag gcgaaaccaa cagccatcaa gattgcggga ataacgacaa tcaacatcag
    52381 gccaaatgcc gtcagtatca gtgaacgttg ctccagacca atctgtcctt tgggatctaa
    52441 cagcgcagaa ttacagccac tgagcaatac agtgcctgca aataatgaca accatcccaa
    52501 acttttattg tatttcctga gtctcattta acgacctcaa ttccacggga tctggtggcg
    52561 tttaaagtgt gtgggcattt tacgggaagg ttacattact gtaaacatga ttaaattcct
    52621 gttacttgga tttggctgtc aggtcacata tgttgcaaaa catatcaagt tgattttaag
    52681 aagctgttgc aaaagggcgt tataacgaaa ggggagtgca gaaggagttt ggacgggcaa
    52741 ttggtataac caatacaaaa taaaacaatt atttaacaaa taattatcat taaggtgaca
    52801 actgggtagt gagaaaataa aaactaatta gctgaatcgt gtaagaaaaa tagaattcat
    52861 aaatagcgtc aataatttcc aaacgctaag ccgcacaaaa gaacaaatat tattaagcga
    52921 tactgatgtt tatttagccg taataattac ggcgagtgat tactacagct aaataatatt
    52981 tacagattac gtcagatgcg tttttcgtag agccagataa tccagcaaac caccaagcac
    53041 gacgccagaa agggcgacta aaaccccgac ttccagcaga gcaggcagga aagagaagtg
    53101 cgtcaaatcc agcgtgtcca tcgtcaacag cagtaaccac acggccaaca ggctgacgcc
    53161 cgccgccagt gtccacatgg caaaggcata acctgccgga tattcggtac gggagataaa
    53221 gttgtcattt actcgtgtat attcaagcgt ctggcggcaa accagcagca aaataagccc
    53281 cggtacggca gcggcgacgg agaatagata gaatgtcgac cagccgtgtg cttcaacaaa
    53341 ccaacccgcc acggggccga cataaactcg tcctacagcg gaaagcgctg agagcagggc
    53401 aaattgagta gcggaaaatg acttattaca cagcgtcatt aacagcgcga caaaggctga
    53461 tgtgcccatc ccgccacaga ggttttcgaa aaagacggct gcgcccatgc tgtagagatg
    53521 cttatcagta atcgacagca gccagtaacc agcgttagac gcaccttgta aaatgccgaa
    53581 aatcagcagc gcccggaaca gcgacaggcg ctgcatcaaa atcccaccgt acaatgcgcc
    53641 aacaatggtc gccagtaagc caagcgtttt gttaaccacg cctacttcac ccgcatcaaa
    53701 cccgacgccg cgaatcaaaa acgtggttgt caggctcatg gcgaatgcgt cgcccagctt
    53761 atacagcacg ataagaagca aaataagcca ggcattattg cgaccaaaga aatctcgcag
    53821 aggtgcaaca accgcttgtt ccagcgtttt tggcacagga atggtgtcgg tcggttctgg
    53881 tgcaagcaac gtcgcgataa tacaggggat caacagcgcc gccatcaacc agtacatgcc
    53941 ctgccagccc agccatttat ctgccagcca cagggctaag ccgccggaaa ccagcatccc
    54001 taaacggtaa cccagaacgc tgattgccgc gcccgcacca cgttcttctg ccgggagcac
    54061 atcggttttc cacgcatcga agacaatatc ctgggaggcc gagcaaaaag cgatcaccac
    54121 cgccagtgcc gccatccagc ggagttgggt gccaggttcg agaaaaccca tcgccgcaat
    54181 ggcgactaat aacaggattt gcgtggcgag cagccaaccg cgccgccgcc caaaaaatgg
    54241 gggcgtgtag cggtccatca gcggtgacca gaggaattta aaaacgtaag cctggcctac
    54301 cagagagaag aaaccaatgg ttttgagatc aatattctcg accgtcatcc aggcctgtaa
    54361 ggtgccggat gtcagggcga gcggtagccc ggaagcaaaa cccaggatca gcaatatggc
    54421 tgaacgcggc tgttgaaaaa tacgtaaata ttgactggac atgggcgtct acaggcccgg
    54481 tttgcaccgg gccagagggc agaaattaac gcgcgttctg cttgataaat tcgtgaatgc
    54541 tggtgtcctg agacatatca gcgatggtat cggtcagcac actgttaacc gcatcggcga
    54601 tatttttgtt ggaggcctgg aacgcacctt caacgttgta gctggcacga tagtttttgg
    54661 tcattttgtt gccattctgc gcggtagcga tgatggcgat atccgctttg gtagcgatgt
    54721 tatagcgcac gttgccctgg gacacgtcag catacagttg gctaacgatg atttgcagat
    54781 taaccgggcc attcggacca accatgtaac cacgcgcggt catctgtttt tccagcactt
    54841 cttgcagcag gaaacgcaga tctcgggagg cggtcagggt aacgatttga ttatcgcggg
    54901 tgacttttgc cagcgcctga tcggtacgct gatcggcacc attaatgctt acggtgacgc
    54961 ccatcaggct tggatcctgc tgtggcagtg taatcgtcgg ggaaacttca atagttgttg
    55021 gcggttttgc gcatcctgca agcataaaca gagcaactaa cgggaagagg atttttttga
    55081 acatgtttgg gctctcagag actcttaagc gtgtttggta aaaattcccg ccatcataac
    55141 attgccaacg gcgaggggaa gtgggtaagg catgtaaatt catcatgttg acgaaataat
    55201 cacccctggt aaaagaaacg ctgatgcgag gcctgtgttt caatctttaa atcagtaaac
    55261 ttcatacgct tgacggaaaa accaggacga aacctaaata tttgttgtta agctgcaatg
    55321 gaaacggtaa aagcggctag tatttaaagg gatggatgac atctcagcgt tgtcggagga
    55381 gatatttcat gatgatacgt gagcggatag aagaaaaatt aagggcggcg ttccaacccg
    55441 tattcctcga agtagtggat gaaagctatc gtcacaatgt cccagccggc tctgaaagcc
    55501 attttaaagt tgtgctggtc agcgatcgtt ttacgggtga acgttttctg aatcgtcatc
    55561 gaatgattta cagtacttta gcggaggaac tctctactac cgttcatgcg ctggctctgc
    55621 atacttacac tattaaggag tgggaagggt tgcaggacac cgtctttgcc tctcctccct
    55681 gtcgtggagc aggaagcatc gcgtaaaaac gcatttgcaa ctgtcggcgc ttttccagta
    55741 tgttgctaaa gattttatga aaaacggcct gcgggccgtt ttgttttgtc tggattttgc
    55801 gctttttgcc cagcattcag acgaaaattg cccgggaatt gtgaaaaaat acgcgacagc
    55861 gcgcaataac cgttctcgac tcataaaagt gatgccgcta taatgccgcg tcctatttga
    55921 atgctttcgg gatgattctg gtaacaggga atgtgattga ttataagaac atcccggttc
    55981 cgcgaagcca acaacctgtg cttgcggggt aagagttgac cgagcactgt gattttttga
    56041 ggtaacaaga tgcaagtttc agttgaaacc actcaaggcc ttggccgccg tgtaacgatt
    56101 actatcgctg ctgacagcat cgagaccgct gttaaaagcg agctggtcaa cgttgcgaaa
    56161 aaagtacgta ttgacggctt ccgcaaaggc aaagtgccaa tgaatatcgt tgctcagcgt
    56221 tatggcgcgt ctgtacgcca ggacgttctg ggtgacctga tgagccgtaa cttcattgac
    56281 gccatcatta aagaaaaaat caatccggct ggcgcaccga cttatgttcc gggcgaatac
    56341 aagctgggtg aagacttcac ctactctgta gagtttgaag tttatccgga agttgaactg
    56401 cagggtctgg aagcgatcga agttgaaaaa ccgatcgttg aagtgaccga tgctgacgtt
    56461 gacggcatgc tggatactct gcgtaaacag caggcaacct ggaaagaaaa agacggcgct
    56521 gttgaagcag aagaccgcgt aaccatcgac ttcaccggtt ctgtagacgg cgaagagttc
    56581 gaaggcggta aagcgtctga tttcgtactg gcgatgggcc agggtcgtat gatcccgggc
    56641 tttgaagacg gtatcaaagg ccacaaagct ggcgaagagt tcaccatcga cgtgaccttc
    56701 ccggaagaat accacgcaga aaacctgaaa ggtaaagcag cgaaattcgc tatcaacctg
    56761 aagaaagttg aagagcgtga actgccggaa ctgactgcag aattcatcaa acgtttcggc
    56821 gttgaagatg gttccgtaga aggtctgcgc gctgaagtgc gtaaaaacat ggagcgcgag
    56881 ctgaagagcg ccatccgtaa ccgcgttaag tctcaggcga tcgaaggtct ggtaaaagct
    56941 aacgacatcg acgtaccggc tgcgctgatc gacagcgaaa tcgacgttct gcgtcgccag
    57001 gctgcacagc gtttcggtgg caacgaaaaa caagctctgg aactgccgcg cgaactgttc
    57061 gaagaacagg ctaaacgtcg cgtagttgtt ggcctgctgc tgggcgaagt tatccgcacc
    57121 aacgagctga aagctgacga agagcgcgtg aaaggcctga tcgaagagat ggcttctgcg
    57181 tacgaagatc cgaaagaagt tatcgagttc tacagcaaaa acaaagaact gatggacaac
    57241 atgcgcaatg ttgctctgga agaacaggct gttgaagctg tactggcgaa agcgaaagtg
    57301 actgaaaaag aaaccacttt caacgagctg atgaaccagc aggcgtaatt tacgcagcat
    57361 aacgcgctaa attcgcacaa aggcccgtca ccgccaggtg gtgggctttt ttttgtcatg
    57421 aattttgcat ggaaccgtgc gaaaagcctc tttcggtgtt agcgtaacaa caaaagattg
    57481 ttatgcttga aatatggtga tgccgtaccc ataacacagg gactagctga taatccgtcc
    57541 ataaggttac aatcggtaca gcaggttttt tcaattttat ccaggagacg gaaatgtcat
    57601 acagcggcga acgagataac tttgcacccc atatggcgct ggtgccgatg gtcattgaac
    57661 agacctcacg tggtgagcgc tcttttgata tctattctcg tctacttaag gaacgcgtca
    57721 tttttctgac tggccaggtt gaagaccaca tggctaacct gattgtggcg cagatgctgt
    57781 tcctggaagc agaaaaccca gaaaaagata tctatctgta cattaactcc ccaggcgggg
    57841 tgatcactgc cgggatgtct atctatgaca ccatgcagtt tatcaagcct gatgtcagca
    57901 ccatctgtat gggccaggcg gcctcgatgg gcgctttctt gctgaccgca ggggcaaaag
    57961 gtaaacgttt ttgcctgccg aattcgcgcg tgatgattca ccaaccgttg ggcggctacc
    58021 agggccaggc gaccgatatc gaaattcatg cccgtgaaat tctgaaagtt aaagggcgca
    58081 tgaatgaact tatggcgctt catacgggtc aatcattaga acagattgaa cgtgataccg
    58141 agcgcgatcg cttcctttcc gcccctgaag cggtggaata cggtctggtc gattcgattc
    58201 tgacccatcg taattgatgc cagaggcgca actgtgccgc tatacttatc cagggcggca
    58261 caacgctgta agcggcttgc gcctgagaat ggcatttgcg tcgtcgtgtg cggcacaaag
    58321 aacaaagaag aggttttgac ccatgacaga taaacgcaaa gatggctcag gcaaattgct
    58381 gtattgctct ttttgcggca aaagccagca tgaagtgcgc aagctgattg ccggtccatc
    58441 cgtgtatatc tgcgacgaat gtgttgattt atgtaacgac atcattcgcg aagagattaa
    58501 agaagttgca ccgcatcgtg aacgcagtgc gctaccgacg ccgcatgaaa ttcgcaacca
    58561 cctggacgat tacgttatcg gccaggaaca ggcgaaaaaa gtgctggcgg tcgcggtata
    58621 caaccattac aaacgtctgc gcaacggcga taccagcaat ggtgtcgagt tgggcaaaag
    58681 taacattctg ctgatcggtc cgaccggttc cggtaaaacg ctgctggctg aaacgctggc
    58741 gcgcctgctg gacgtcccgt tcaccatggc cgacgcaacc acgctgaccg aagccggtta
    58801 tgtgggcgaa gacgttgaaa acatcattca gaaactgttg cagaaatgcg actacgatgt
    58861 ccagaaagca cagcgtggta ttgtctacat cgatgaaatc gacaagattt ctcgtaagtc
    58921 ggacaacccg tctattaccc gtgacgtttc cggtgaaggc gtacagcagg cactgttgaa
    58981 actgatcgaa ggtactgttg ctgccgttcc accgcagggc gggcgtaagc atccgcagca
    59041 ggaatttttg caggttgata cctccaagat cctgtttatt tgtggcggtg cgtttgccgg
    59101 tctggataaa gtgatttccc accgtgtaga aaccggctcc ggcattggtt ttggcgcgac
    59161 ggtaaaagcg aagtccgaca aagcaagcga aggcgagctg ctggcgcagg ttgaaccgga
    59221 agatctgatc aagtttggtc ttatccctga gttcattggt cgtctgccgg ttgtcgcaac
    59281 gttgaatgaa ctgagcgaag aagctctgat tcagatcctc aaagagccga aaaacgccct
    59341 gaccaagcag tatcaggcgc tgtttaatct ggaaggcgtg gatctggaat tccgtgacga
    59401 ggcgctggat gctatcgcta agaaagcgat ggcgcgtaaa accggtgccc gtggcctgcg
    59461 ttccatcgta gaagccgcac tgctcgatac catgtacgat ctgccgtcca tggaagacgt
    59521 cgaaaaagtg gttatcgacg agtcggtaat tgatggtcaa agcaaaccgt tgctgattta
    59581 tggtaagccg gaagcgcaac aggcatctgg tgaataatta accattccca tacaattagt
    59641 taaccaaaaa ggggggattt tatctcccct ttaatttttc ctctattctc ggcgttgaat
    59701 gtgggggaaa catccccata tactgacgta catgttaata gatggcgtga agcacagtcg
    59761 tgtcatctga ttacctggcg gaaattaaac taagagagag ctctatgaat cctgagcgtt
    59821 ctgaacgcat tgaaatcccc gtattgccgc tgcgcgatgt ggtggtttat ccgcacatgg
    59881 tcatcccctt atttgtcggg cgggaaaaat ctatccgttg tctggaagcg gcgatggacc
    59941 atgataaaaa aattatgctg gtcgcgcaga aagaagcttc aacggatgag ccgggtgtaa
    60001 acgatctttt caccgtcggg accgtggcct ctatattgca gatgctgaaa ctgcctgacg
    60061 gcaccgtcaa agtgctggtc gaggggttac agcgcgcgcg tatttctgcg ctctctgaca
    60121 atggcgaaca cttttctgcg aaggcggagt atctggagtc gccgaccatt gatgagcggg
    60181 aacaggaagt gctggtgcgt actgcaatca gccagttcga aggctacatc aagctgaaca
    60241 aaaaaatccc accagaagtg ctgacgtcgc tgaatagcat cgacgatccg gcgcgtctgg
    60301 cggataccat tgctgcacat atgccgctga aactggctga caaacagtcc gttctggaga
    60361 tgtccgacgt taacgaacgt ctggaatatc tgatggcaat gatggaatcg gaaatcgatc
    60421 tgctgcaggt tgagaaacgc attcgcaacc gcgttaaaaa gcagatggag aaatcccagc
    60481 gtgagtacta tctgaacgag caaatgaaag ctattcagaa agaactcggt gaaatggacg
    60541 acgcgccgga cgaaaacgaa gccctgaagc gcaaaatcga cgcggcgaag atgccgaaag
    60601 aggcaaaaga gaaagcggaa gcagagttgc agaagctgaa aatgatgtct ccgatgtcgg
    60661 cagaagcgac cgtagtgcgt ggttatatcg actggatggt acaggtaccg tggaatgcgc
    60721 gcagcaaggt caaaaaagac ctgcgtcagg cgcaggaaat ccttgatacc gaccattatg
    60781 gtctggagcg cgtgaaagat cgcatccttg agtaccttgc ggttcaaagc cgtgtcaaca
    60841 aaatcaaggg accgatcctc tgcctggtag ggccgccggg ggtaggtaaa acctctcttg
    60901 gtcagtccat tgccaaagcc accgggcgta aatatgtccg tatggcgctg ggcggcgtgc
    60961 gtgatgaagc ggaaatccgt ggtcaccgcc gtacttacat cggttctatg ccgggtaaac
    61021 tgatccagaa aatggcgaaa gtgggcgtga aaaacccgct gttcctgctt gatgagatcg
    61081 acaaaatgtc ttctgacatg cgaggcgatc cggcctctgc actgcttgaa gtgctggatc
    61141 cagagcagaa cgtagcgttc agcgaccact acctggaagt ggattacgat ctcagcgacg
    61201 tgatgtttgt cgcgacgtcg aactccatga acattccggc accgctgctg gatcgtatgg
    61261 aagtgattcg cctctccggt tataccgaag atgaaaaact gaacatcgcc aaacgtcacc
    61321 tgctgccgaa gcagattgaa cgtaatgcac tgaaaaaagg tgagctgacc gtcgacgata
    61381 gcgccattat cggcattatt cgttactaca cccgtgaggc gggcgtgcgt ggtctggagc
    61441 gtgaaatctc caaactgtgc cgcaaagcgg ttaagcagtt actgctcgat aagtcattaa
    61501 aacatatcga aattaacggc gataacctgc atgactacct cggtgttcag cgtttcgact
    61561 atggtcgcgc tgataacgaa aaccgtgtcg gtcaggtaac cggtctggcg tggacggaag
    61621 tgggcggtga cttgctgacc attgaaaccg catgtgttcc gggtaaaggc aaactgacct
    61681 ataccggttc gctcggcgaa gtgatgcagg agtctattca ggcggcgtta acggtggttc
    61741 gtgcgcgtgc ggaaaaactg gggatcaacc ctgattttta cgaaaaacgt gacatccacg
    61801 tccacgttcc ggaaggtgcg acgccgaaag atggtccgag tgccggtatt gctatgtgca
    61861 ccgcgctggt ttcttgcctg accggtaacc cggttcgtgc cgatgtggca atgaccggtg
    61921 agatcactct gcgtggtcag gtactgccga tcggtggttt gaaagaaaaa ctcctggcag
    61981 cgcatcgcgg cgggattaaa acagtgctaa ttccgttcga aaataaacgc gatctggaag
    62041 agattcctga caacgtaatt gccgatctgg acattcatcc tgtgaagcgc attgaggaag
    62101 ttctgactct ggcgctacaa aatgaaccgt ctggtatgca ggttgtgact gcaaaatagt
    62161 gacctcgcgc aaaatgcact aataaaaaca gggctggcag gctaattcgg gcttgccagc
    62221 ctttttttgt ctcgctaagt tagatggcgg atcgggcttg cccttattaa ggggtgttgt
    62281 aaggggatgg ctggcctgat ataactgctg cgcgttcgta ccttgaagga ttcaagtgcg
    62341 atataaatta taaagaggaa gagaagagtg aataaatctc aattgatcga caagattgct
    62401 gcaggggctg atatctctaa agctgcggct ggccgtgcgt tagatgctat tattgcttcc
    62461 gtaactgaat ctctgaaaga aggggatgat gtagcactgg taggttttgg tacttttgcc
    62521 gttaaagagc gtgctgcccg tactggccgc aacccgcaga ccggtaaaga gatcaccatc
    62581 gctgctgcta aagtaccgag cttccgtgca ggtaaagcac tgaaagacgc ggtaaactaa
    62641 gcgttgtccc cggtggggat gtgacgaagt tcaagggcgc atctactgat gtgccttttt
    62701 tatttgtatt cggtgacttt ctgcgtcttg tgggctgaca attgcccccg tttcttgtca
    62761 caataggcct ttgcgcgcat cgatacgttg cgtgaggtac acagtcatct acagcggagt
    62821 gttgttacac catgatggac agcttacgca cggctgcaaa cagtctcgtg ctcaagatta
    62881 ttttcggtat cattatcgtg tcgttcatat tgaccggcgt gagtggttac ctgattggcg
    62941 gaggcaataa ctacgccgca aaagtgaatg accaggaaat cagccgtggg cagttcgaga
    63001 acgcctttaa cagcgagcgt aatcgcatgc agcaacagct gggcgatcaa tactccgagc
    63061 tggcagcgaa tgaaggctat atgaaaaccc tgcgtcaaca ggtgctgaat cgtctgatcg
    63121 acgaggcgct gttggatcag tacgctcgtg agctgaaact gggtatcagt gatgagcagg
    63181 ttaaacaggc gattttcgcg accccagcct tccaggttga cggcaaattt gataacagcc
    63241 gctataacgg tatcctcaac cagatgggga tgaccgccga tcagtacgcc caggcgctgc
    63301 gtaaccagct cactacccaa cagctgatta acggtgttgc cggtaccgat tttatgctga
    63361 aaggtgaaac cgacgagctg gcggcactgg tcgcgcaaca acgcgtggtg cgtgaggcga
    63421 ctatcgatgt taacgagctg gcggcgaagc agcctgtgac cgaacaggag attgccagct
    63481 actacgaaca aaacaaaaac aatttcatga cgccggaaca attccgcgtg agttacatca
    63541 agctggatgc cgcaacgatg cagcaaccgg ttagcgatgc ggatatccag agctactacg
    63601 atcagcatca ggatcaattc acccagccgc agcgtacccg ctacagcatc atccagacca
    63661 aaactgaaga tgaagcgaaa gcggtacttg atgagctgaa taaaggcggt gattttgctg
    63721 cattagccaa agaaaaatct gccgatatta tctctgcccg taacggcggc gatatgggtt
    63781 ggttagaaga tgccactatc cccgacgaac tgaaaaatgc tggtctgaaa gaaaaagggc
    63841 aactgtctgg tgtcatcaaa tcttcggtcg gtttcctgat tgtacgtctg gacgacattc
    63901 agccagcgaa agtgaagtcg ttagacgaag tgcgtgacga tgtcgcggcg aaagtgaaac
    63961 acgaaaaagc cctcgatgcg tactatgcgc tgcagcagaa agtgagcgat gcggcaagca
    64021 acgacaccga gtctctggcc ggtgcagagc aagcagccgg cgttaaagcc actcagacgg
    64081 ggtggtttag caaagataac ctgccggaag agttgaactt caagccggtt gccgacgcta
    64141 tctttaacgg cggtctggta ggtgaaaacg gcgcgccagg catcaactct gacatcatca
    64201 ccgtagatgg cgaccgcgca ttcgtgctgc gcatcagcga gcacaaaccg gaagtggtga
    64261 aaccgttggc agatgttcag gaacaagtta aggcactggt tcagcacaac aaagctgaac
    64321 aacaggcgaa agtggatgct gagaaactgc tggttgattt gaaagccggc aaaggtgcgg
    64381 aagctatgca ggctgccggt ctgaaatttg gcgagccgaa aaccttaagc cgttccggtc
    64441 gtgacccgat tagccaggcg gcgtttgcac tgccactgcc agcgaaagac aaaccgagct
    64501 acggtatggc gaccgatatg caaggtaatg tggttctgct ggcgctggat gaagtgaaac
    64561 aaggttcaat gccggaagat cagaaaaaag cgatggtgca gggtatcacc cagaacaacg
    64621 cacaaatcgt ctttgaagct ctgatgagta acctgcgtaa agaggcgaaa atcaaaattg
    64681 gcgatgcgct ggaacagcaa taatcctgaa gccgcctcgc aaaaaaatgc gttgcagctg
    64741 taacaagaaa aggtcgcttt cgcggccttt tccatttctg aacattgcca tttgtttact
    64801 gttttcactg ccgttaaggt gattccactg ttaacaaaca aggagaaaac agtatgaaac
    64861 acggaattaa agcactgctc attaccctgt ccctggcctg tgccggaatg tctcatagcg
    64921 cgctggcggc agcttctgtg gcgaaaccga cggcggtaga aaccaaagcg gaagctcctg
    64981 cagcacaaag taaagcagca gtaccggcga aagccagtga cgaagaaggc acccgggtca
    65041 gcattaataa tgccagcgcg gaagagctag cccgcgcgat gaatggcgtt ggcctgaaga
    65101 aagcgcaggc gattgtcagt tatcgcgaag agtacggtcc gtttaaaacg gtggaggatc
    65161 taaagcaggt gccggggatg ggcaattcgc tggtggaacg taatctggcg gtattaaccc
    65221 tgtaattaat ttgcatagtg gcaatttttt gccagactga agaggtcata ccagttatga
    65281 cctctgtact tataacaaca acgtaaggtt attgcgctat gcaaacacaa atcaaagttc
    65341 gtggatatca tctcgacgtt taccaacacg tcaacaacgc ccgctacctt gaatttctcg
    65401 aagaagcccg ctgggatggg ttggaaaata gcgacagttt tcagtggatg acggcccata
    65461 acatcgcctt cgtcgtggtc aatatcaata ttaactatcg tcgcccagcg gtattaagcg
    65521 acctgttaac tattaccagt cagttgcagc aattaaacgg taaaagcggc atcttaagcc
    65581 aggtcattac actggagccg gaagggcagg tggtagcgga tgcgcttatt acgtttgttt
    65641 gtattgatct taaaacgcag aaagcattag ctctggaagg ggaattgcgc gaaaagctgg
    65701 agcagatggt taagtaaacg ttttgtggtg ccggatgctc aagccgcatc cggcgacacc
    65761 cggaataatt acctcaaccc ggttttctgc ttcatcgctg ccatcaccgt cggtttatcg
    65821 gccagataat gattcaaacc gttggcgcgt aaattacatg ccgcacaatg accgcaaccg
    65881 tcgcctttaa tgccgttata gcaggtcaac gtttcgttac ggactaaatc cagtttgccg
    65941 taataatctg ccagcgccca ggtttccgct ttatcaatcc acatcagcgg cgtttcaaaa
    66001 cgaatatctt tcgccatgcc caaactgacg gcatggttta gtgctttcac aaactcatcg
    66061 cggcaatccg ggtagccgga gaaatccgtt tcacagacgc cggtaattac ggcttctgct
    66121 tttacctgat acgcatatat tgccgccagc gtcaggaaca aaatattacg ccctgggaca
    66181 aacgtattcg ggatgccatc ggcttcaggt tcataatcag gcaccggaat gctgtcacgt
    66241 gtcaggctgc tgaccgccag ctcgttgagc agcgttacat caagaacctt atgcgcgcgt
    66301 gctcccagtt tcagcgccag ttcgcgtgcc acgtcgattt ctgcgcgatg tcgctgaccg
    66361 taatcgaacg tcacgcaatg gacttcatca tattgttgta atgcctgcac cagacaggtg
    66421 gtggaatcct ggcctccact gaacacaacg acagcacgtt tcatagataa tcctgcttga
    66481 acaataaaag cgttatggta acgcctgcga ttaacccgga ccagctattc attgcgacgg
    66541 cgcgggaagc caggcttcgg taaattcaaa ccagccgcgc ggtgtcagtc gcacaccgtt
    66601 cacgccggga ggggcactaa tgcgatagtg atagttgaac agcggcgtca gcgtcgcgtc
    66661 tgccattaac tggctaaaaa ccgctttcag ggcattaaat cggttttcct catcaggcat
    66721 tacttgcacc gcgtccagcg tcgattgcag atgtgcatat gctggagcgt cgaaaacatg
    66781 tggccatagt ggatcgcaac gtagccattg ctccagagta tattccggtg cttcgccaat
    66841 taatctgtcg cccatcatga ggtctgcgtg tgccagtagg gtcgtgtcgt cccagttttt
    66901 tgcgttatga aaaataattg tgagttcaca gccttccgct gccagtgtcg cctgtaggcg
    66961 ttctgccatg gtatgaagtt ctatcggtaa gtgataaacc agcgtcaagg ttttcggtag
    67021 tttgacttca tccggtactt gccagtgcgg aatagtccag cctggcagta atgcatgact
    67081 ggcggtgatc aggttttctc cgacttctaa cgtttgtaat aaaccggatt gatgaataat
    67141 ggagatcact tttcgcgcct gccagaggga gagtcgggga cttttgcgca acgttaaata
    67201 gcaaaaacct aaactgatgc cactactgac ctggctgacc cgttgcagct cctccggttt
    67261 gccgatggtg atttgcacgg gatgccgaca actggttccc aaatcttttt cgaaaagcgg
    67321 cggagttatc cagtactcaa ccgctttaag cagcggatga cgcaaatggt aataatcatg
    67381 gctctccagg cgcaccagct ccgctgtgaa ttgtgttaag cgaaaaggac cagtgccgat
    67441 cagtgggaat tgcggatgcg ccagatggct gcaatagctc gccagccggt gcgcaagcca
    67501 gtaatcgggg cgatgtaaaa agaaggtcag acactgcgga tgggtgactt caatacgctt
    67561 cacgctaata aataattgat ccagtgctgg cagttgtaac agcatcaata atcgctggtg
    67621 taagtgtgag gcttttactg catcgccgtt atgccagtgt agggttgaac gaagataaaa
    67681 gtcccagcgt aacccgtcag tagaggtttc ccagtgatgc gctaaatcgc caatcgggcg
    67741 ctgagtatta ttatcgaagc gggtcaggcc ggaaaatatc tgcccggcga gatgctgctc
    67801 ggcacggccg ggcaaaaagc ctggttgtag cggttcgagc gggcgatagt agggaatacg
    67861 caacgtgggt gtatcgtttt gccattgtcc gcccataaac ggctgtaaca gagtgcgcag
    67921 ctcacctggg gccagttgcg ccagctccag cacatcttgc tgctttccgg tttccagtgc
    67981 ctgttccatc atcgcattgc gtagcgattc cggcgtgacc agaaagcgta attgtccgcg
    68041 ctttccgcgt cctgactgcg cctgccactc cagccatccc gcctcctgtg cctgacgcaa
    68101 cagcgtacga acatggcgtt cgctgcaaaa acagcgttcg gccagttcgc tgacggtgac
    68161 ggtttgcggc tttccggctg aaggttgcca cagacgttga tactggttaa gacggttgag
    68221 caatcgcata taaacccgga acaatattat ttaactattc actattactt ccgtatatat
    68281 caggtgatac tcaatcacca ttaaccgtgt cacagagtgg agaaagaaat ggctcgtctg
    68341 gcagcatttg atatggatgg cactttattg atgcctgacc atcatttagg tgagaaaacc
    68401 ctctctactt tggcgcgact gcgtgaacgc gacattaccc tcacttttgc cacggggcgt
    68461 catgcgctgg agatgcagca tattctcggg gcgctatcgc tggatgcata tttgattacc
    68521 ggcaacggaa cgcgcgtgca ttctctggaa ggtgaacttt tacatcgtga tgatttacct
    68581 gcggatgtcg cggagctggt gctgtatcag caatgggata cccgagccag catgcatatc
    68641 ttcaatgacg acggttggtt taccgggaaa gagatccctg cgttgttgca ggcatttgtc
    68701 tatagcggtt ttcgttatca gataatcgat gtcaaaaaaa tgccactcgg cagcgtcacc
    68761 aagatctgct tctgtggcga tcacgacgat cttacacgct tgcagatcca gctatacgaa
    68821 gcattaggcg agcgtgcaca tttgtgtttt tccgccacgg attgcctcga agtgctgccg
    68881 gtgggctgca ataaaggcgc tgcattgacg gtgctgaccc aacatttagg tttatcgttg
    68941 cgcgattgca tggcctttgg tgatgcgatg aacgatcgcg aaatgttagg cagcgtcggt
    69001 agcggattta ttatgggcaa tgcgatgccg caactgcgcg cggagctccc gcatttaccg
    69061 gtgattggac attgccgaaa tcaggctgtc tctcactatt tgacgcactg gctggactat
    69121 ccacatctac cttattcccc cgaataacga gatcccttcc agcaccgggc aattgcccgg
    69181 ttttttttgc gttgagtttg tcattttgtg ccatggtgtt taaaccgcac agaataaatt
    69241 gtcgcgattt cacctttaaa atagaattaa aagagaaaaa aattctctgt ggaagggcta
    69301 tgttagataa aattgaccgt aagctgctgg ccttactaca gcaggattgc accctctctt
    69361 tgcaggcact ggctgaagcc gttaatctga caaccacccc ctgctggaag cgcctgaaac
    69421 ggctggagga cgacggtatc cttatcggca aagtcgccct gctggatccg gaaaaaatag
    69481 gcctcggcct gaccgccttt gtgctgataa aaacgcaaca tcacagcagc gaatggtatt
    69541 gccgctttgt cacggtggtt accgaaatgc cagaagtgct ggggttctgg cgcatggctg
    69601 gtgaatacga ttatctgatg cgcgtccagg ttgccgacat gaaacgctac gacgagtttt
    69661 ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt cacttccagc ttcgcgatgg
    69721 aacagattaa atacaccact tctttaccca tcgaataaat atccagaatc aggtcaggac
    69781 acaacgcgtg cgattatttg ctcaattaag ctggtatttc cgtcgggaat ggcgtcgcta
    69841 tctcggggct gtcgccttgc ttgtcattat cgcgatgctg caactggttc cgccaaaagt
    69901 ggttggtatt gttgtcgatg gcgtgacaga acaacacttt actaccgggc agatcctgat
    69961 gtggatcgcc accatggtgc tgattgccgt tgtggtttat ctcctgcgtt acgtctggcg
    70021 cgtattgctg tttggtgcgt cttatcaact ggctgttgaa ctgcgtgaag attattaccg
    70081 tcagctaagc cggcagcatc ctgagtttta cctgcgtcat cgcacgggtg acctcatggc
    70141 tcgtgcgact aatgacgtcg atcgcgtcgt gtttgccgcc ggagaagggg tgctgacgct
    70201 ggtggattca ctggtaatgg gctgcgctgt gttgattatg atgtctacgc aaattagctg
    70261 gcagttgacc ttatttgccc tgttaccgat gccagtgatg gcgatcatga ttaagcgcaa
    70321 cggcgatgcc ttgcatgaac gctttaagct ggcacaggcg gcgttttcta gtcttaatga
    70381 ccgcacccag gaaagcctca ccagtattcg catgatcaaa gcctttggtc tggaagatcg
    70441 ccagtcggcg ttatttgccg cggatgccga agataccggc aaaaaaaaca tgcgggtggc
    70501 gcgtattgat gctcgtttcg acccgaccat ctatatcgcg attggtatgg cgaacttgct
    70561 ggcgattggc ggcggtagtt ggatggtggt gcagggcagt ttaacgctgg gccagctcac
    70621 cagttttatg atgtatttag gtctgatgat ttggccaatg ctggcgctgg catggatgtt
    70681 taacattgtg gaacgtggta gtgctgcgta cagccgtatt cgcgcgatgc tggcggaagc
    70741 gccggtggtg aacgatggta gcgaacccgt gccggaaggg cgtggcgaac tggatgtaaa
    70801 tattcaccag ttcacgtatc cgcagactga ccatcctgcg ctggaaaacg tcaatttcgc
    70861 cctgaaaccc ggtcagatgc tgggtatttg cgggccgact ggttccggca aaagtaccct
    70921 gttgtcgctc attcagcgtc atttcgacgt cagcgagggg gatattcgct ttcatgatat
    70981 tcctctgacg aagttacaac tcgatagctg gcgtagccgc ctggcggtag ttagccagac
    71041 accgttcctt ttttccgaca ccgtagcgaa caacatcgcg ttgggttgcc cgaatgccac
    71101 ccagcaagag attgagcatg tcgcgcggtt agccagcgta catgacgata ttttgcgttt
    71161 accgcaaggt tacgatacag aggtgggcga gcgcggcgtg atgctttccg gcgggcaaaa
    71221 acagcgtatc tctattgctc gtgcgttatt agtcaacgcg gaaatcctca tccttgatga
    71281 tgcgctttcg gcggtggacg gacgcacaga gcaccagatc ctgcataacc tgcgtcagtg
    71341 ggggcaggga agaacggtaa tcatcagtgc ccatcgcctt tcggcactga cggaagccag
    71401 tgaaattatt gtgatgcagc acggacatat cgcccagcgt ggcaatcatg atgagctggc
    71461 gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa caactggagg cggcgctcga
    71521 cgacgttccg gaaattcgcg aggaggccgt cgatgcgtag ttttagccaa ctgtggccga
    71581 ctctcaagcg tctgttagcg tacggttcgc cgtggcggaa accgctgggg attgcggtcc
    71641 tgatgatgtg ggttgcggca gcggcagaag tcagtgggcc gctgcttatc agctatttta
    71701 tcgacaatat ggtagcgaaa aataacctgc cgttgaaaat ggttgcaggg ctggcggcgg
    71761 cgtatgttgg gctacaactg tttgccgccg ggctacatta cgcgcagtcg ctgctgttta
    71821 atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat gctgcgttac
    71881 gccagccatt aagcgagttt gatacccaac ccgtcgggca ggtgatttcc cgcgtcacta
    71941 atgacactga agtgatccgc gatctctacg ttaccgtagt ggcaactgtc ctgcgtagcg
    72001 ccgcactggt gggcgcgatg ctggtggcga tgttcagcct cgactggcga atggcgctgg
    72061 tggcgataat gattttcccg gtggtgctgg tggtaatggt gatataccag cgttacagca
    72121 cgccgattgt ccgtcgtgtg cgcgcctatc tagcggatat caacgacggc tttaacgaaa
    72181 tcatcaatgg catgagcgtt atccagcagt tccgtcagca ggcgcgattt ggcgaacgta
    72241 tgggggaggc cagtcgttca cactatatgg ccaggatgca aaccctgcgc ctcgacggtt
    72301 ttctgctgcg tccgctgctg agtctgtttt catcgctcat tctttgtggc ttgttgatgc
    72361 tgtttggctt ctccgctagc ggcaccattg aagtgggcgt gctgtatgcg tttatcagct
    72421 atcttgggcg acttaacgaa ccattaatcg aactgaccac gcaacaggcg atgctgcaac
    72481 aggctgttgt tgctggtgag cgcgtgtttg aactgatgga cggaccgcgc cagcaatatg
    72541 gcaatgatga tcgcccgtta cagagtggca ccatcgaagt cgataacgtg tcatttgctt
    72601 atcgcgatga caatctggtg ctaaagaaca ttaatctctc tgtgccttcg cgtaactttg
    72661 tggcactggt cgggcatacc ggcagtggca aaagcaccct cgccagttta ttgatgggct
    72721 attacccgct aacggaaggt gagattcgcc ttgatggtcg tccattaagt tcgctaagtc
    72781 acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg ctggcggata
    72841 ccttcctcgc caacgtgacg ctggggcgtg atatctccga agaacgcgtc tggcaggcgc
    72901 tggaaaccgt gcaactggcg gagctggcgc gcagcatgag tgatggtatt tatacgccgc
    72961 tgggcgagca ggggaataat ctctccgtcg ggcaaaagca actgctggca ctggcacgcg
    73021 tgttggtgga gacgccgcaa atcctgatcc ttgatgaggc aaccgccagc attgactccg
    73081 gtactgaaca ggcgattcaa catgctctgg cggctgtgcg tgaacatacc acgctggtgg
    73141 tgattgctca ccgcttatcg accattgttg atgccgacac cattctggtg cttcatcgtg
    73201 ggcaagccgt ggagcagggc actcaccagc aactgctggc ggcccaggga cgctactggc
    73261 agatgtatca actgcaactt gcgggcgaag agctggcagc cagcgtgcgt gaagaagaat
    73321 cattgagcgc ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa cgcactgtgc
    73381 actgtcatag tgcgttttca ttttcaatct tcttaacttc ctgctctctt tctcgttttt
    73441 catttctggc acaccgcttg caataccttc ttcgtgtagc agaaccatta ccgaattctg
    73501 accggagggg atctatgaag ctggtgaccg tgataatcaa accattcaag ctggaagacg
    73561 ttcgtgaagc gttatcttcc attggtattc agggcctgac cgtcaccgaa gtgaaaggtt
    73621 tcgggcgtca gaaagggcat gccgagctgt accggggggc ggaatacagc gtcaacttcc
    73681 tgccaaaagt aaaaattgat gtggcgattg ctgatgacca actcgatgaa gtgatcgata
    73741 tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg caaaatcttc gtcgctgaat
    73801 tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga agcggcgctg taatctctgg
    73861 cacacagcaa caggaacgaa aaatgaagat agcgacgata aaaactgggc ttgcttcact
    73921 ggcgatgctg ccgggactgg taatggctgc acctgcggtg gccgataaag ccgacaatgc
    73981 gtttatgatg atttgtactg cgctggtgct gtttatgact attccgggga ttgccctgtt
    74041 ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg ctgacgcagg tgacggtgac
    74101 gtttgcactg gtctgtattc tctgggtggt ttacggttac tcgctggcgt ttggtgaggg
    74161 caacaacttc ttcggcaaca ttaactggtt gatgctaaaa aacatcgaac tgacggcggt
    74221 gatgggcagc atttatcagt atatccacgt ggcgtttcag ggctcgtttg cctgcattac
    74281 cgtcggcttg atcgttgggg cgctggcgga acgaatccgc ttctcagctg tgttgatttt
    74341 cgtggtggta tggctgacgc tctcttacat tccgattgcg catatggtgt ggggcggtgg
    74401 tttgctggct tctcacggtg cgctggattt cgcgggtggc acggtggtgc atattaacgc
    74461 cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct tcggcaaaga
    74521 ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt actgccattc tctatattgg
    74581 ttggtttggc tttaacgccg ggtcagcggg cacggcgaat gaaatcgcgg cactggcatt
    74641 tgtgaatact gtggtcgcaa cggcggcggc aattcttggc tggatcttcg gtgaatgggc
    74701 gctgcgtggt aagccttctc tgctgggagc gtgttctggc gcgattgccg gtctggtcgg
    74761 cgtgacgcca gcgtgtggct acattggggt tggcggcgcg ttgattatcg gcgtggtagc
    74821 tggtctggcg ggcttgtggg gcgttactat gctcaaacgc ttgctgcggg tggatgatcc
    74881 ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc ggctgtatca tgactgggat
    74941 ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa ggtgtgacga tgggccatca
    75001 gttgctggta cagctggaaa gcatcgccat tacgatcgtc tggtccggtg tggtggcatt
    75061 tattggctac aaactggcgg atctgacggt tggtctgcgt gtaccggaag agcaggagcg
    75121 agaagggctg gatgtcaaca gtcacggcga gaatgcctat aacgcgtaac aagcactgca
    75181 aaaaatagcc ggacggtttt acctccggct atttttttaa ttgtgattac gcatcacccc
    75241 ttcctgaacg gtcgaggcaa ccagcacgcc gtcctgggta taaaactcac cgcgcacaaa
    75301 gccgcgtgcg ctggacgccg aggtgctctc cacgctatac agcagccatt cattcaaatt
    75361 aaacgggcga tggaaccaca tggaatggtc aatggtggca atctgaatcc ccggttcgag
    75421 aaaaccgatg ccgtgcggct gtagagctac cggcaggaag ttaagatcag aagcgtaacc
    75481 gagcagatac tgatgaacgc gcaggtcatc cggcacgcta ccatttgcgc ggatccacac
    75541 ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta tgaaactcca ccggacggac
    75601 ttccagcgga cgatcgcaga tgaatttatc tttaagcact ggcggcagca ggtgcgccag
    75661 cgattgggcg atttgcgttt ccgaaggcag gccatcaggc gctggcgcgg acggcattgt
    75721 tttttgatgt tcgaaacccg cttctggtgc ctggaaagag gccgtcatat aaaaaatcgg
    75781 tttgccgttt tgaatagcag caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt
    75841 ttcgacatca taaataatcg gcttcttact atcgccaggg cgaagaaagt agctgtgaaa
    75901 cgaatgtacc agccgctctt cagggacggt ctcttttgca gcatacaagg cctgacccac
    75961 gacctggccg ccaaacacct ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc
    76021 ttcctcaatt ttttccagat ttaacaatgt cagtaaattt tttagcgcct gactcatata
    76081 actctccagt aacaaagctg ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg
    76141 ctactggtcc gatgggtgca atggtctgaa ttacgggcta attacaggca gaaatgcgtg
    76201 atgtgtgcca cacttgttga cgttactatt ttgttaacca ctcttccggc gaggaaagtt
    76261 agcccgctgg tgcattgata ataaggagaa atgaatgaaa ctcgtgcaca tggccagtgg
    76321 tttagcggtt gcgattgcgt tggcggcttg cgcagataaa agcgcggata ttcagacgcc
    76381 agccccggct gcaaatacat ctatttcagc aacacaacaa cctgctatcc agcaaccgaa
    76441 tgtctccggt accgtctgga tccggcagaa agtcgcactg ccgcctgatg ctgtgctgac
    76501 cgtgacactt tctgacgcgt cgttagccga tgcaccgtca aaagtgctgg cgcagaaagc
    76561 ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt ctgccattta atccggcaga
    76621 tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt accgtgaatg acaaactggt
    76681 atttatcacc gataccgttc agccggtgat caaccagggc ggaactaaag ccgacctgac
    76741 attggtgccg gtacagcaaa ccgccgtgcc ggttcaggcc agcggtggtg caacgactac
    76801 cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg gcagttccag ctcctacgca
    76861 atattaagcg acgttaaccc tctccgcccg gagagggtta gtagttccag cgataacgct
    76921 gcaagtcgat ttgcccgctt cccgatacca tcacaccttc tgccagtaat gcctgtcgct
    76981 gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg gcgattaacc acccggtgcc
    77041 agggtaaggt gctgccttca gggagacgct ttaacacacc gcccacctgg cgcgcggcgc
    77101 ggggcgatcc cgccagtttc gccacatcac cgtaagtggt gacatagcct tcgggaatag
    77161 cggcgacgat ttgccagacg cgttggggaa atgaatcttc tttttccatc ttttcttcct
    77221 gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg aagtcgcatt gcgcaagaaa
    77281 ccagcatctg gcacgcgatg ggttgcaatt agccggggca gcagtgataa tgcgcctgtg
    77341 cgttggttct caacgctctc aatgggggct ctgttggttc tcccgcaacg ctactctgtt
    77401 taccaggtca ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc
    77461 tggcagggcc cccacccatt tctgcctccc accgtttcgt caaaaaaccc caacatggct
    77521 aaactttaac cacaactgac gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt
    77581 atgtctgatg aagtatgttg atggttttgt ggttgccgtt cctgccgata aaaaggatgc
    77641 ctatcgggaa atggccgcta aggctgcgcc attgtttaaa gagtttggcg cgcttcgtat
    77701 tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg accgattttc gtatggcggt
    77761 gaaagcggaa gagaatgaag aggttgtctt tagctggatt gaataccctt caaaagaggt
    77821 ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg atgaaagagt tcggcgagtc
    77881 catgccgttt gacggcaagc gaatgatcta tggcggattc gagtcaatca tcgacgaata
    77941 ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa attcacaaat gttgctcggc
    78001 ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag agccagccct gaccgtagtg
    78061 cacgccatgc tggcgtagcc actcttcttg tttactggtt tcgattccct ccgctaccat
    78121 tttcagtttc agtgtttttg ccatttcgat gatatgcggc gtgacatttt tatattccag
    78181 cgcatcaacg aaagatttat cgatcttcag aatgtcgaca tccagatcct gtaaataact
    78241 taaacttgaa tatcccgtac caaaatcatc aagataaatt tcatggcccg cctcccggta
    78301 gcgagaaatt atcggggcgc tggttttcgg atcggcaaac tcgcgttcag tgagttcaag
    78361 cgcgatctgt ctgggattaa cctgatagtg attgatcatt tcacgcagca attgcgggat
    78421 tttttccgag gtgagcacgg tggattcaag attgatcgaa atatgctgct gtggatgctg
    78481 acgcagccag tcgcccatat cttcaaagac gcttcttata atcagtagcg tcaatggctc
    78541 agaaaggccc gtttgctgtg ccagcggaat aaaactatct ggtgacaacc aactaccgtc
    78601 tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt ttgccattgg ctaaggagac
    78661 aatcggctga tagtgcacgc aaatatcacg attttcgata gcatcctgca gccgatgatg
    78721 cggtgactgg atacggcgca aaatacgcag cacaaacatc gctgccagca ggccaatcac
    78781 caacccgagc ggtaaccaaa taaagacttg ccgatgccag cctttctgca acattttcgt
    78841 tgaagcccag gtgatgatcg aaatattcat ctccgggaag ggcaggatat cgtaaatgat
    78901 tccattattt tcgatatgct caccgggtgt tttttgtagc ctggtaataa ttccctgagc
    78961 aatttcatcg ctgctggtta tgacaacgtt atgggcattg ccaataatgg cggcatcaat
    79021 ttgccatgag ctatagggaa tgacatcaat aaaggaagcg gggtcgatca tgacgacata
    79081 atgtgccgtt cccatggcga ccatgtaacg gataatgcct aaatcgttat gcgatgttaa
    79141 ccagacacga taaccatctt tcgaaatttt acctggctcg gggaaggtat cgggcggact
    79201 ttcatgctcc agagacgaac actggggaac gttgttatcg atataaacca cttcctgaat
    79261 atagcgataa ctgtaagata cccgacgcat ttccatgaga tgggcttcgc tacaggcagc
    79321 gccttgccat ctttccagct cttgcagcgc atctttccct tgtgtcgcca ccttattggc
    79381 ccgaatagcg acgcgggagg aataggtatc cagctcttca ataaacgatg tttctacctg
    79441 ctgatgggcc agccagatgc ttaagccgac aggcagcaat actgaaagaa tcagtactcc
    79501 cgaaataagg ccgaccagat gtcgtgttct caccagcata tccttattaa cacagtgtag
    79561 ggaaagtata tctgattgtg gaaaaaggct ggcaggagat aaggaggtag agctggtatt
    79621 tcatgaagtg atgggtggaa acgatcattg tcatttgacc aggcgcagag ctttacgcct
    79681 ggtatgtaac cctaacttaa gatgttgatt cggcgcgagc gagggtaaaa atatcgtaaa
    79741 aagacagttc acctttacgg acgatcattt tttgcagttg ttccttatga acatccgcga
    79801 cacgcggaga gttcaagatg gcttcaacca gcgcggcagg tacaaagcgc gtgttgtacc
    79861 attcgccgtt atagcaaacg cggaaatcca gcacgtcgat atcttcataa cggtagcgtg
    79921 ggtagacatc gaaaaagaag aaaccattaa gcaggataaa taatacaacc agtagccaga
    79981 cagagcctga cagcgtttcg gactgcaaca tcaccgccag ggtggcaaaa aaagcaacgt
    80041 acataccgat aaacagcccc ggatgtttac ggataaaact gatactaaag cggggtttgt
    80101 tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc ggtcagcagg cgttgtattt
    80161 cggtcatcgt ttgccttcgt ggtatctgca actttccagg gaacacccta ttttaacgtg
    80221 ggaatagtag aggggaagta acagatttac cacaattttg cataacagtt gcgaaaaacc
    80281 gattacaatt ttttaattat tctggctgga ttaccgccca cgacaacgtt gttcgggaca
    80341 tcttttgtga caactgcacc tgaggctacc acgacgttat caccaatggt cacaccaggg
    80401 ttaatgaccg cgcgtccgcc aatccagacg ttattaccga tggtgacggg tttccccagt
    80461 tcagcaccgc tattacgtgc tacagggtcg atgggatgtg ttgccgtgta gatatgaacg
    80521 cctggtgcca acatacagtt atcaccgatg cgaatagggc agacatcaag catcacgcaa
    80581 tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat agccatagtc acagcgaaac
    80641 gttggctcaa tataagcctc tgtcacctga ccgaatagat cagcgagaat ttgctggcgt
    80701 aatgtgtgct cttccgccag ggaatgattg tatcggtgaa taagctgacg agcgcgcagg
    80761 cgatcgcgag ataacgtctc atctgccgag cgatacaact caccagcaat catcttttct
    80821 ttttctgtgc tcattactga cctccggttg ctgaaaatgc ggcaacacag taagacgaaa
    80881 agtcagtgaa gagaacgttc ccattacatc tgtgtgataa agatcacata gggtatgtct
    80941 tcatggcgaa aaagtataaa attcttaata aacagccggt tatagctccg aaagcgaatt
    81001 atttattagc gaataaattt ccatactgag gaagggatct tgtcgtacag tttattcatg
    81061 gtcaattcgg cgaggcggtg atctgcggct gagtaaaata ccgccagttc attatctgat
    81121 aattcgtatt tatttttctc gataacacgc tccagcgtgt caattgtctg gcaacgacgt
    81181 aaacgcatta aataatcggt tttcgttaaa ggtttttcgg acatacttct acccatggtt
    81241 gtaataattc taacaagata aactcgcagg attctctttc gtcgcattga caaaacaacg
    81301 gaatagtcga ttacctgact tccgccattt ctgaagatcc tgcatattaa taccataact
    81361 actgaacaac ataaaggtgt catccagata ttcgtcgatc tgctcaatga gcttattgtc
    81421 ttcattatac ttaattttgt aattaagtgc gaaggtcgca atatgctcaa tcagttcatt
    81481 caactggagg ttgatcgccg aggttgggtc gttaacccag ccatgattgc tttcttcaag
    81541 gtttgcaagg cagtcatgat acagggtttc acagagaaac ttaagctgtg cgatatcatg
    81601 tct
//
LOCUS       DAFAVF010000004        77720 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFAVF010000004 DAFAVF010000000
VERSION     DAFAVF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.004"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            291..1664
                     /gene="aroP"
                     /locus_tag="LGK42_000233"
     CDS             291..1664
                     /gene="aroP"
                     /locus_tag="LGK42_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBK2656522.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1707..2561)
                     /gene="ampE"
                     /locus_tag="LGK42_000234"
     CDS             complement(1707..2561)
                     /gene="ampE"
                     /locus_tag="LGK42_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBK2656523.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2558..3109)
                     /gene="ampD"
                     /locus_tag="LGK42_000235"
     CDS             complement(2558..3109)
                     /gene="ampD"
                     /locus_tag="LGK42_000235"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBK2656524.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3197..4090
                     /gene="nadC"
                     /locus_tag="LGK42_000236"
     CDS             3197..4090
                     /gene="nadC"
                     /locus_tag="LGK42_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBK2656525.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4293..4733
                     /gene="ppdD"
                     /locus_tag="LGK42_000237"
     CDS             4293..4733
                     /gene="ppdD"
                     /locus_tag="LGK42_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBK2656526.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4743..6128
                     /gene="gspE"
                     /locus_tag="LGK42_000238"
     CDS             4743..6128
                     /gene="gspE"
                     /locus_tag="LGK42_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBK2656527.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6118..7320
                     /gene="hofC"
                     /locus_tag="LGK42_000239"
     CDS             6118..7320
                     /gene="hofC"
                     /locus_tag="LGK42_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBK2656528.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7355..8398)
                     /gene="guaC"
                     /locus_tag="LGK42_000240"
     CDS             complement(7355..8398)
                     /gene="guaC"
                     /locus_tag="LGK42_000240"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK2656529.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8554..8598)
                     /locus_tag="LGK42_000241"
     CDS             complement(8554..8598)
                     /locus_tag="LGK42_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656530.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8623..9243
                     /gene="coaE"
                     /locus_tag="LGK42_000242"
     CDS             8623..9243
                     /gene="coaE"
                     /locus_tag="LGK42_000242"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK2656531.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9243..9986
                     /gene="zapD"
                     /locus_tag="LGK42_000243"
     CDS             9243..9986
                     /gene="zapD"
                     /locus_tag="LGK42_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBK2656532.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9996..10193
                     /gene="yacG"
                     /locus_tag="LGK42_000244"
     CDS             9996..10193
                     /gene="yacG"
                     /locus_tag="LGK42_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBK2656533.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10293..10682)
                     /gene="mutT"
                     /locus_tag="LGK42_000245"
     CDS             complement(10293..10682)
                     /gene="mutT"
                     /locus_tag="LGK42_000245"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBK2656534.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10742..13447)
                     /gene="secA"
                     /locus_tag="LGK42_000246"
     CDS             complement(10742..13447)
                     /gene="secA"
                     /locus_tag="LGK42_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK2656535.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13509..14021)
                     /gene="secM"
                     /locus_tag="LGK42_000247"
     CDS             complement(13509..14021)
                     /gene="secM"
                     /locus_tag="LGK42_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBK2656536.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14252..15169)
                     /gene="lpxC"
                     /locus_tag="LGK42_000248"
     CDS             complement(14252..15169)
                     /gene="lpxC"
                     /locus_tag="LGK42_000248"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBK2656537.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15270..16421)
                     /gene="ftsZ"
                     /locus_tag="LGK42_000249"
     CDS             complement(15270..16421)
                     /gene="ftsZ"
                     /locus_tag="LGK42_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK2656538.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16482..17744)
                     /gene="ftsA"
                     /locus_tag="LGK42_000250"
     CDS             complement(16482..17744)
                     /gene="ftsA"
                     /locus_tag="LGK42_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK2656539.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17741..18571)
                     /gene="ftsQ"
                     /locus_tag="LGK42_000251"
     CDS             complement(17741..18571)
                     /gene="ftsQ"
                     /locus_tag="LGK42_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBK2656540.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18573..19493)
                     /gene="ddlB"
                     /locus_tag="LGK42_000252"
     CDS             complement(18573..19493)
                     /gene="ddlB"
                     /locus_tag="LGK42_000252"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK2656541.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELVGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19486..20961)
                     /gene="murC"
                     /locus_tag="LGK42_000253"
     CDS             complement(19486..20961)
                     /gene="murC"
                     /locus_tag="LGK42_000253"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK2656542.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21015..22082)
                     /gene="murG"
                     /locus_tag="LGK42_000254"
     CDS             complement(21015..22082)
                     /gene="murG"
                     /locus_tag="LGK42_000254"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK2656543.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22079..23323)
                     /gene="ftsW"
                     /locus_tag="LGK42_000255"
     CDS             complement(22079..23323)
                     /gene="ftsW"
                     /locus_tag="LGK42_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBK2656544.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23323..24639)
                     /gene="murD"
                     /locus_tag="LGK42_000256"
     CDS             complement(23323..24639)
                     /gene="murD"
                     /locus_tag="LGK42_000256"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK2656545.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGLTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24642..25724)
                     /gene="mraY"
                     /locus_tag="LGK42_000257"
     CDS             complement(24642..25724)
                     /gene="mraY"
                     /locus_tag="LGK42_000257"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK2656546.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25718..27076)
                     /gene="murF"
                     /locus_tag="LGK42_000258"
     CDS             complement(25718..27076)
                     /gene="murF"
                     /locus_tag="LGK42_000258"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK2656547.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27073..28560)
                     /gene="murE"
                     /locus_tag="LGK42_000259"
     CDS             complement(27073..28560)
                     /gene="murE"
                     /locus_tag="LGK42_000259"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBK2656548.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28547..30313)
                     /gene="ftsI"
                     /locus_tag="LGK42_000260"
     CDS             complement(28547..30313)
                     /gene="ftsI"
                     /locus_tag="LGK42_000260"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBK2656549.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30329..30694)
                     /gene="ftsL"
                     /locus_tag="LGK42_000261"
     CDS             complement(30329..30694)
                     /gene="ftsL"
                     /locus_tag="LGK42_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK2656550.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30691..31632)
                     /gene="rsmH"
                     /locus_tag="LGK42_000262"
     CDS             complement(30691..31632)
                     /gene="rsmH"
                     /locus_tag="LGK42_000262"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK2656551.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31634..32092)
                     /gene="mraZ"
                     /locus_tag="LGK42_000263"
     CDS             complement(31634..32092)
                     /gene="mraZ"
                     /locus_tag="LGK42_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK2656552.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32694..33698)
                     /gene="cra"
                     /locus_tag="LGK42_000264"
     CDS             complement(32694..33698)
                     /gene="cra"
                     /locus_tag="LGK42_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBK2656553.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33780..33866)
                     /locus_tag="LGK42_000265"
     CDS             complement(33780..33866)
                     /locus_tag="LGK42_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBK2656554.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33878..34369)
                     /gene="ilvN"
                     /locus_tag="LGK42_000266"
     CDS             complement(33878..34369)
                     /gene="ilvN"
                     /locus_tag="LGK42_000266"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBK2656555.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34372..36096)
                     /gene="ilvI"
                     /locus_tag="LGK42_000267"
     CDS             complement(34372..36096)
                     /gene="ilvI"
                     /locus_tag="LGK42_000267"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBK2656556.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36215..36259
                     /locus_tag="LGK42_000268"
     CDS             36215..36259
                     /locus_tag="LGK42_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656557.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36414..37358)
                     /gene="leuO"
                     /locus_tag="LGK42_000269"
     CDS             complement(36414..37358)
                     /gene="leuO"
                     /locus_tag="LGK42_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBK2656558.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            38018..38104
                     /gene="leuL"
                     /locus_tag="LGK42_000270"
     CDS             38018..38104
                     /gene="leuL"
                     /locus_tag="LGK42_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBK2656559.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            38197..39768
                     /gene="leuA"
                     /locus_tag="LGK42_000271"
     CDS             38197..39768
                     /gene="leuA"
                     /locus_tag="LGK42_000271"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBK2656560.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            39768..40859
                     /gene="leuB"
                     /locus_tag="LGK42_000272"
     CDS             39768..40859
                     /gene="leuB"
                     /locus_tag="LGK42_000272"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBK2656561.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            40862..42262
                     /gene="leuC"
                     /locus_tag="LGK42_000273"
     CDS             40862..42262
                     /gene="leuC"
                     /locus_tag="LGK42_000273"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBK2656562.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            42273..42878
                     /gene="leuD"
                     /locus_tag="LGK42_000274"
     CDS             42273..42878
                     /gene="leuD"
                     /locus_tag="LGK42_000274"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBK2656563.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(42927..44105)
                     /gene="setA"
                     /locus_tag="LGK42_000275"
     CDS             complement(42927..44105)
                     /gene="setA"
                     /locus_tag="LGK42_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBK2656564.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(44207..44338)
                     /gene="sgrT"
                     /locus_tag="LGK42_000276"
     CDS             complement(44207..44338)
                     /gene="sgrT"
                     /locus_tag="LGK42_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBK2656565.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            44427..46082
                     /gene="sgrR"
                     /locus_tag="LGK42_000277"
     CDS             44427..46082
                     /gene="sgrR"
                     /locus_tag="LGK42_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBK2656566.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      46116..46215
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            46246..47229
                     /gene="thiB"
                     /locus_tag="LGK42_000278"
     CDS             46246..47229
                     /gene="thiB"
                     /locus_tag="LGK42_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBK2656567.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAANWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            47205..48815
                     /gene="thiP"
                     /locus_tag="LGK42_000279"
     CDS             47205..48815
                     /gene="thiP"
                     /locus_tag="LGK42_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBK2656568.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLCHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            48799..49497
                     /gene="thiQ"
                     /locus_tag="LGK42_000280"
     CDS             48799..49497
                     /gene="thiQ"
                     /locus_tag="LGK42_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBK2656569.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(49611..50375)
                     /locus_tag="LGK42_000281"
     CDS             complement(49611..50375)
                     /locus_tag="LGK42_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2656570.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(50461..51339)
                     /gene="araC"
                     /locus_tag="LGK42_000282"
     CDS             complement(50461..51339)
                     /gene="araC"
                     /locus_tag="LGK42_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBK2656571.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            51678..53378
                     /gene="araB"
                     /locus_tag="LGK42_000283"
     CDS             51678..53378
                     /gene="araB"
                     /locus_tag="LGK42_000283"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBK2656572.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            53389..54891
                     /gene="araA"
                     /locus_tag="LGK42_000284"
     CDS             53389..54891
                     /gene="araA"
                     /locus_tag="LGK42_000284"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK2656573.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            55060..55755
                     /gene="araD"
                     /locus_tag="LGK42_000285"
     CDS             55060..55755
                     /gene="araD"
                     /locus_tag="LGK42_000285"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK2656574.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            55830..58181
                     /gene="polB"
                     /locus_tag="LGK42_000286"
     CDS             55830..58181
                     /gene="polB"
                     /locus_tag="LGK42_000286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBK2656575.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            58346..61252
                     /gene="rapA"
                     /locus_tag="LGK42_000287"
     CDS             58346..61252
                     /gene="rapA"
                     /locus_tag="LGK42_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBK2656576.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            61264..61923
                     /gene="rluA"
                     /locus_tag="LGK42_000288"
     CDS             61264..61923
                     /gene="rluA"
                     /locus_tag="LGK42_000288"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBK2656577.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(62040..62855)
                     /gene="djlA"
                     /locus_tag="LGK42_000289"
     CDS             complement(62040..62855)
                     /gene="djlA"
                     /locus_tag="LGK42_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBK2656578.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            63110..65464
                     /gene="lptD"
                     /locus_tag="LGK42_000290"
     CDS             63110..65464
                     /gene="lptD"
                     /locus_tag="LGK42_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBK2656579.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            65517..66803
                     /gene="surA"
                     /locus_tag="LGK42_000291"
     CDS             65517..66803
                     /gene="surA"
                     /locus_tag="LGK42_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBK2656580.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            66803..67792
                     /gene="pdxA"
                     /locus_tag="LGK42_000292"
     CDS             66803..67792
                     /gene="pdxA"
                     /locus_tag="LGK42_000292"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBK2656581.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            67789..68610
                     /gene="rsmA"
                     /locus_tag="LGK42_000293"
     CDS             67789..68610
                     /gene="rsmA"
                     /locus_tag="LGK42_000293"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK2656582.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            68613..68990
                     /gene="apaG"
                     /locus_tag="LGK42_000294"
     CDS             68613..68990
                     /gene="apaG"
                     /locus_tag="LGK42_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBK2656583.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            68997..69839
                     /gene="apaH"
                     /locus_tag="LGK42_000295"
     CDS             68997..69839
                     /gene="apaH"
                     /locus_tag="LGK42_000295"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBK2656584.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(69917..70396)
                     /gene="folA"
                     /locus_tag="LGK42_000296"
     CDS             complement(69917..70396)
                     /gene="folA"
                     /locus_tag="LGK42_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBK2656585.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(70588..72450)
                     /gene="kefC"
                     /locus_tag="LGK42_000297"
     CDS             complement(70588..72450)
                     /gene="kefC"
                     /locus_tag="LGK42_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBK2656586.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARTAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(72443..72973)
                     /gene="kefF"
                     /locus_tag="LGK42_000298"
     CDS             complement(72443..72973)
                     /gene="kefF"
                     /locus_tag="LGK42_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBK2656587.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(73081..74412)
                     /locus_tag="LGK42_000299"
     CDS             complement(73081..74412)
                     /locus_tag="LGK42_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656588.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(74471..74758)
                     /gene="fixX"
                     /locus_tag="LGK42_000300"
     CDS             complement(74471..74758)
                     /gene="fixX"
                     /locus_tag="LGK42_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBK2656589.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(74755..76041)
                     /locus_tag="LGK42_000301"
     CDS             complement(74755..76041)
                     /locus_tag="LGK42_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2656590.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(76092..77033)
                     /locus_tag="LGK42_000302"
     CDS             complement(76092..77033)
                     /locus_tag="LGK42_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBK2656591.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(77048..>77720)
                     /gene="fixA"
                     /locus_tag="LGK42_000303"
     CDS             complement(77048..>77720)
                     /gene="fixA"
                     /locus_tag="LGK42_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative electron transfer flavoprotein FixA"
                     /protein_id="HBK2656592.1"
                     /translation="ISQYDLNAIEAACQLKQQAAEAQVTALSVGGKALTNAKGRKDVL
                     SRGPDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVG
                     EILNIPAVNGVSKIISLAADTLTVERELEDETETLSIPLPAVVAVSTDINSPQIPSMK
                     AILGAAKKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGDGEEQIAAFAEN
                     LRKVI"
BASE COUNT        18688 a        21539 c        19758 g        17735 t
ORIGIN      
        1 ttaccaattt tgtgattttt aattcaatta aaacgaattt aaattcattc tacatattga
       61 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
      121 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
      181 gtagcaattc aaacttcttt gatgtaaaca aattaataca acaaacggaa ttgcaaactt
      241 acacacgcat cactgcgtag atcaaaaaaa caaccaccgc acgaggtttc atgatggaag
      301 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
      361 cgctgggtgg cgcgataggg accgggttat tcctgggtag cgcctccgta atacagtccg
      421 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
      481 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
      541 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
      601 ttttagttgc catggctgag ctgactgccg tgggtaaata cattcagttc tggtatccgg
      661 aaatccccac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
      721 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttatcgcgg
      781 tggtagcgat gatcatcttc ggcggctggc tgctattcag tggcaacggc ggcccgcagg
      841 cgaccgttag caacctgtgg gatcagggtg gtttcctgcc gcacggcttc accgggctgg
      901 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
      961 cagcagaagc tgataacccg gagcaaagta taccgaaagc aactaaccag gttatctacc
     1021 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
     1081 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
     1141 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
     1201 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc
     1261 tggcgtctgt cgataaacgt ggtgtaccag taaataccat tctggtgtct gcactggtaa
     1321 cggcgttgtg cgtactgatt aactaccttg ccccagagtc cgctttcgga ctgttaatgg
     1381 cgctggtggt atctgcactg gtaatcaact gggcgatgat tagcctggcg catatgaaat
     1441 tccgtcgcgc caagcaggaa caaggcgtgg taactcactt ccctgctctg ctttatccgc
     1501 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
     1561 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
     1621 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
     1681 cccgtacagg gtgagggcaa taatctttac accaacgccc cgtaaatcgt cagtagtgca
     1741 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
     1801 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaacacc
     1861 tgatactgcg aagtatggaa atcacccagc gaagcaaacc aggctggtaa cgctttctca
     1921 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
     1981 tgaagcacgg catcaatgcc ggactgtaaa cgatgatgcg gcgtctgata tcgcgccagc
     2041 cagtattgcc atgcacgcaa aaacgcatac cccatcagcg taacgggtcc ccaggttccc
     2101 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
     2161 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
     2221 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
     2281 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
     2341 cacaccagta gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
     2401 gtcacgccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
     2461 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
     2521 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
     2581 gcacacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
     2641 tatcacaatg gcccgtcatg tttttagcga tatccggata gcaatcaatc agtgcccgcg
     2701 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagcgtatcg gtgccttcaa
     2761 gctcaatccc aatagaaaaa tcattgcagc gttcgcgccc ctgatactga gagactcccg
     2821 catgccatgc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
     2881 aacagtgagc ggagacgcgc aaatgggcga tttcagcaaa gaaaggatgt gcctgcggat
     2941 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
     3001 taatattgtg caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg
     3061 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
     3121 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgtttgc
     3181 tatctggagt tttaacatgc cgcctcgccg ctataaccct gacacccgac gtgacgagct
     3241 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
     3301 aggcggaaca gtcgatgcca acaatgatat tacggcaaaa cttttaccgg aaaattctcg
     3361 ctctcatgcc acggtgatca cccgcgagaa tggcgtcttt tgcggcaaac gctgggttga
     3421 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
     3481 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
     3541 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
     3601 acgccactat gtcgaattgc tggaaggcac caacacgcag ttgttggata cgcgcaaaac
     3661 cttacccggc ctgcgttcag ctctgaaata cgcggttctt tgcggcggcg gagcgaatca
     3721 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
     3781 agtgcgccag gcggtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga
     3841 agtagagaat ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct
     3901 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
     3961 actggaagtg tctggcaacg tcactgacaa aacactgcgt gaatttgccg aaacgggcgt
     4021 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
     4081 ttttcgctaa tagttgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
     4141 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
     4201 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
     4261 agtagcgcca actaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
     4321 tcgaactgat ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt
     4381 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
     4441 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
     4501 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
     4561 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacaccgg
     4621 gttgggataa cgcaaacggc gtcaccggct ggacgcgcaa ctgcaatatt caaagtgaca
     4681 gcgcattgca tcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
     4741 caatgaatat tccacagctc actgccctgt gtctgcgtta tcatggagtc ttgctggatg
     4801 ccagcgaaga ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ctactggacg
     4861 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
     4921 aaggtcacgc cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc
     4981 ccaaagcaga gttgctaact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
     5041 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
     5101 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc
     5161 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
     5221 tggcaggaaa cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
     5281 tggtattaag gttgttacag caggtgagcc aggcactgga tgtcaacacg cttggaatgc
     5341 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
     5401 taactggccc tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaacgctga
     5461 ataccgctga cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac
     5521 taaaccagac gcaaatccat ccgcgtgccg gactcacctt tcagggcgtg ttgcgtgcgt
     5581 tattgcgcca ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacagcag
     5641 agatcgctat taaagcggcg caaactggtc acctggtgtt gtctacccta cacactaatt
     5701 ccacctgcga aacgctggta cgtttacagc aaatgggggt cgcccgctgg atgctatcat
     5761 cggcgcttac gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc
     5821 ggcagcaagg ggagcccatc cacattccag acaatgtatg gccatcgcca ctgccccact
     5881 ggcaggcacc cggttgtgta cattgctacc acggttttta tggtcgtacg gccttatttg
     5941 aagttctgcc cataacgccg gtcattcgtc agcttatttc cgctaatacc gacgttgaat
     6001 cgctggaaac gcacgcacga caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg
     6061 ccgtggagca aggcttaacc acctttgaag agttaatccg cgtactgggg atgccgcatg
     6121 gcgagtaagc aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg
     6181 atgctatggg cagagagccg tgctttgctg ctcatggcac tacagcaaca gatggttacc
     6241 ccacttagcc tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg
     6301 gaagtcattc atcaactggc gacgctactc aaagccgggt taacgctttc tgaagggctg
     6361 gcactgctgg cggaacagca tcccagtaag caatggcaag cgttgctgca atcgctggcg
     6421 cacgatctcg aacagggcat tgctttttcc aatgccttat taccctggtc agaggtattt
     6481 ccgccactct atcaggcgat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc
     6541 tgctttgaac tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca
     6601 gcgttacgtt atcccatcat cattttagcg atggcaatca tggtggttgt ggcaatgctg
     6661 cattttgttc tgccggagtt tgccgctatc tataagacct tcaacacccc actaccggca
     6721 ctaacgcagg ggatcatgac gctggcagac tttagtggcg aatggagctg gctgctggtg
     6781 ttgttcggct ttctgctggc gatagccaat aagttgctga tgcgccgacc gacctggctt
     6841 atagtgcggc agaaattgct gttacgaatc ccgattatgg gttcactgat gcggggacaa
     6901 aaactcacgc agatctttac gattctggcg ctgacacaaa gtgcaggcat tacttttttg
     6961 cagggcgtag agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca
     7021 caaatccagc acgatatcag taacggtcat cccatctggc tggcgctaaa aaataccggg
     7081 gagtttagcc cgctctgttt gcaattagtg agaacaggag aggcatccgg ctcgctggac
     7141 ctcatgttag acaacctcgc ccatcatcat cgggataaca caatggcgct ggcggataac
     7201 ctcgcagcct tactggaacc ggcgttgctg atcataacgg gaggaattat cggtacgctg
     7261 gtggtggcga tgtatctgcc aattttccat ttaggcgatg cgatgagtgg gatgggataa
     7321 cgctggcgtg ttgctccaca ccagcgttgg aagattacag gttgttgaag atgcggtttt
     7381 cttgttcctg cacacggata aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc
     7441 caacgtatgt acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg
     7501 ggcctcgcag cggcagctta acggttttac cttctgctgc gcgatattcc gcaacgccgc
     7561 caacgtgacg tttcatcgca gactcggagc tcatgccgta gaacagcata aatttctcgc
     7621 cgttctcctc aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca
     7681 tgacgaaatc ggcaccgccg ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac
     7741 cgtcgctgac gatcattccg cccagaccgt gcgccgcatc ggcacattcg attaccgcag
     7801 aaagctgcgg ataaccgacg cctgttttga cgcgagttgt gcaaacagaa cctgggccaa
     7861 tgccaacttt aacgatatca gcaccggaga ggataagctc ctcacacatt tcaccagtca
     7921 ctacgttgcc agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact
     7981 gcacgaagtg ttcggaataa ccattcgcca catcaataca aacgaagttt aatgccgggt
     8041 tcaggtcgag aatctgttta gttttttcga aatccgcatc agacgtaccg gtagaaacca
     8101 tcacatgttt cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag
     8161 aatagtgttt atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa
     8221 atgtacctac ggtgtccata tttgcggcga taattggcac gccggaccag ctctgacctg
     8281 aatgtttgaa ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc
     8341 gtttagggcg gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt
     8401 gcggattcct ggggttaatg gctataaggg taagagcgca actccagtga cgctatcata
     8461 cgcactaatc aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata
     8521 aatttacctg gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cactcatttt
     8581 taacgttttg ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc
     8641 ttaacgggag gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga
     8701 attaacgtca ttgatgccga tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg
     8761 ctacatgcca ttgctgatca ctttggcgct aacatgattg ctgctgatgg aacattgcag
     8821 cgccgggcct tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgcc
     8881 ctgctgcatc cgctgattca gcaagagacg caacaccaga tccagcaagc tacttccccc
     8941 tatgtactgt gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga
     9001 gtgctggtgg tggatgtcag cccagaaacg caacttaagc gcaccatgca gcgcgatgat
     9061 gtaactcgcg agcatgtcga acaaatcctt gctgctcagg caacgcgcga agcccgcctt
     9121 gccgtggcag atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt
     9181 gcccgcctgc acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg
     9241 taatgcagac ccaggtcctt tttgaacatc cactaaatga aaaaatgcgt acatggctgc
     9301 gcattgagtt tttgattcag caactcaccg ttaatttacc catcgttgac cacgctggcg
     9361 cgctgcattt cttccgtaat gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc
     9421 gcactgagct gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg
     9481 tgcctggcgt ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga
     9541 gcgtattaat ttccgcgccg cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc
     9601 tggtgcgtca gcgactgagc atcccaggcg gctgttgcag ctttgattta cctacattgc
     9661 acatttggct gcatctaccc caggcgcagc gcgacagcca ggtagaaacc tggattgcca
     9721 gcctgaaccc gctcacccag gcacttacca tggtgctgga tttaattcgc cagtcggctc
     9781 ccttccgtaa acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact
     9841 tgctgcgcct gaatctgtcg ctcgattcac agctttatcc gcaaatttcc ggacataaga
     9901 gccgttttgc cattcgtttt atgccgctgg acactgaaaa cggacaggta ccggaacgtc
     9961 tggatttcga actggcctgt tgctaaggag tgaaaatgtc agaaactatt acggtgaatt
    10021 gcccaacctg cgggaaaacg gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct
    10081 ccaaacgttg tcagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca
    10141 gcagtggcga tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacatttgt
    10201 ctgatgccgc acgtaggcct gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa
    10261 atgtcggatg cggcgtgaac gccttatccg acctacagac gcttaagctt cgctattacc
    10321 ggttcattgg ctggcggaaa atcatcggca ttaagaccga ctagcgacat ccactcaccg
    10381 ggttgccctt ctttacccca cggctcccct tcccagcttt cgaccagcca gaaccacaga
    10441 gttatatgcc tgtccgggaa ttcatattcc agtttttcaa atagcgaaaa atgttgtggg
    10501 gtaatcccga cttcttcctg aagttcacgc accaccgcct gttccggcgt ttcgcccatt
    10561 tcaattttac cgccgggaaa ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc
    10621 gttataaaga tttcattgtt ctcgttgcga ataataccta ccgcaatttg cagctttttc
    10681 attgtcttaa acctataaaa aaggcgcaga atcctgcgcc ttttacttca actgttagct
    10741 tttattgcag gcgaccatgg cactgcttgt attttttacc agaaccgcac gggcaaggat
    10801 cgttacgtcc tactttgcgc tcaccggttt gcgccgccag tgcagctgcg gctgcagagt
    10861 cgtcatcctg atggctaagc tgctgcattt gcgctaaacg ctcggcttcc atacgacgct
    10921 gttgttccag ctcctcaacc tcttcaggca tacgtacctg gactttgctc agcgtgctga
    10981 taacttcata ttttaacgac tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt
    11041 attcctgctt cggatctttc tgtgcatagc cacgcaggtg gataccctga cgcagatagt
    11101 ccatcgctgc caggtgctct ttccacaggg agtcaagcgt ttgcagcatg acgcctttct
    11161 cgaagtgacg catcatctca gcaccaacca cttcttcttt acgctgatac acttcgatgg
    11221 actgcgccag aatgcgctca cgcagcgtct cttcatgcag ttctggttct ttatccagcc
    11281 actcggcaat tggtaaatcg aggtcgaaat cgttcttcag acgttcctgc agccccggaa
    11341 tatcccacat ttcttccagc gactgtggcg gaatgtaggc atcaatggtg gctttgaaca
    11401 catcttcacg aatgctgttg atggtttcgc tcacatcgct gacatccagc agttcgttac
    11461 gctgggagta aatggcgcga cgctgatcgt tagccacgtc atcatattcc agcagttgct
    11521 tacgaatgtc gaagttacgg ctttcaactt tacgctgggc gttggcaatc gctttagtca
    11581 cccacgggtg ttcaatggct tcgcctggct tcatacccag tttacgcatc atgccggata
    11641 ctcggtcgga agcaaaaata cgcatcagcg catcttccat cgacaggtag aaacgggaag
    11701 aaccagcatc cccctgacga ccagaacgac cgcgcaactg gttatcgata cgacgggatt
    11761 cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc cagtaccgca tcgtgacgta
    11821 cctgccagtc ggctttaatt ttttcaattt gctctgcggt cggattttcc agcgcggcaa
    11881 cttctgcctg ccagctacca ccgagcacaa tatctgtacc acgacccgcc atattggtcg
    11941 cgatagtcac cgcagccgga taacctgcct gagcaacaat cgccgcttcg ttggcgtgga
    12001 atttggcgtt caggacgttg tgcttaatac cggctttggt cagttcgttt gacaccagct
    12061 ccgatttttc gatggagata gtacccacca gcaccggctg gcctttcgca gtacgttctt
    12121 tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt catgtagacc aggtccggca
    12181 gatctttacg aatcattgga cggttggtcg gaacaacgac ggtatccagc ttgtagatgg
    12241 agctaaattc gaaagcttcg gtatcagcag taccggtcat ccccgccagt ttttcataca
    12301 gacggaagta gttctggaag gtgatcgaag ccagcgtttg gttttcgttc tggatctgca
    12361 caccttcttt cgcttccaca gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg
    12421 tacgaccggt gtgttcgtca acgatgataa cttcaccatc tttaacgatg tagtcgacgt
    12481 cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt
    12541 tggccggaga gtacagagac tccccttcat ccatgatgcc ctctttcacc agcagttctt
    12601 caatcagcac cagaccacgt tcggtcaggt tcacctggcg agatttttcg tccaccgaga
    12661 agtggccttc gccctggaag gtttcggagt cttctttttc ctgacggatc aggtgcggaa
    12721 taattttatt cacgcgttta tacatttccg agctgtcttc tgccgggccg gaaatgatca
    12781 gcggtgtacg cgcttcatcg atcaggatgg agtccacttc gtccaccagc gcatagtgca
    12841 gtttacgctg tacacgttct tcagggctga acgccatatt gtcgcgcagg tagtcaaagc
    12901 cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta agcttcgcgc tttgccggtg
    12961 ctggcatgcc cggcaggttg ataccgacag tcaggccaag gaattcaaac agcggacggt
    13021 tgttttcggc gtcacgttgc gccaggtagt cgttgacggt aactacgtgc acgcctttac
    13081 cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag ggttttacct tcaccggtac
    13141 gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc gagtaactga acgtcgaagt
    13201 gacgcatacc aaagacgcgc ttacttgcct cacgtaccac ggcgaaagct tccgggatca
    13261 gattttccag cacttcgcct ttttccagac gtgcacgaaa ctctgcggtt ttccctttca
    13321 gttcttcgtc ggagagtttt tccatctccg gttccatggc attgatgatg ttgaccactt
    13381 tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa aactttagtt aacaatttga
    13441 ttagcataat aaaatctcaa acgccccgcg ttgcggagtt aataaaatga agtaaaggtt
    13501 tattgttgtt aggtgaggcg ttgagggcca gcacggatgc cttgcgcctg gcttatccag
    13561 acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat aatcaatgcg ataacccttt
    13621 tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt
    13681 tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa
    13741 gaaagatgac ggattaccgt gcgaatggca tgttgatgcc agtaatcaac ggaatagttc
    13801 gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt gaccaaagtt aactttggct
    13861 gaaggctcgt ggttgcgggt tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg
    13921 agcgcaggca aacctaaact cgccgcaacc atccctaata agagatgcgg ccagaagtag
    13981 cgtttaccaa actgtcgcca gcgcgtcagt attccactca cgttattgcc atcccgatcg
    14041 tattcaaacg tatttagcgc acaaatctta tcattaaagc ctgcagtcca cagcacgaat
    14101 gaagataagt tgtgcggaaa agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct
    14161 cttataaaat tcaaaaaaaa acgactggtt cacctggccg gagagagtgc cagatttgcc
    14221 agtcgaattt tatacgacag tataaatgtc gttatgccag tacagctgaa ggcgctttga
    14281 aggccaacgg cagttctgcg tcgtcctgga aggtcacata ttcccaggct tcctgtttcg
    14341 ccaggacagc ctgcagcagt ttgttattca gtgcatgacc ggatttataa gcggtaaatg
    14401 caccaataat attgtgacca cacatgaaca agtcaccgat cgcatcgagc attttgtgac
    14461 gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa
    14521 cgatggcaca atcgaagctg ccgcccaggc acaaaccacg ggactgcaga tattcgatat
    14581 cacgcatgaa accgaacgta cgcgcacggc tgatctggcg cataaacgca tcagcggaga
    14641 agttcatcgc atagcgctgg ttgctggaat caatagccgg atggttaaaa tcgatggtga
    14701 aatccagcga aaaaccattg tacggcttaa attcagccca cttatcgcca tcttcgacac
    14761 gaacagtctc tttgatgcga acaaattttt tggcgcagtt caactcgtcg ataccggcgt
    14821 caagcagcag gtatacaaac ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt
    14881 taacttcgat aacaatgtta tcgatgccca agcccgcgag agcagcattg aggtgctcta
    14941 cggttgaaat ccgtacatca tgctcgttga ccagacacgt acagagcatg gtatcacgca
    15001 cagatttggc atcggccggg aaatctaccg gtggattcaa gtcggtgcga cgatagatga
    15061 ccccggtgtt ggccggcgca gggcgtaacg tcagggtgac tttcttgccg gtatgtaaac
    15121 cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg tttgatcatc gtattatctc
    15181 gccaaattac ctatccaacc gaagtgtact atacattcgg cgggccagtt tagcacaaag
    15241 agcctcgaaa cccaaattcc agtcaattct taatcagctt gcttacgcag gaatgctggg
    15301 atatccagat aatccggctc tttcgcagtt tgcggcgcat tgtcattcac gactttagca
    15361 accggcttct gctcctgggt cagcggagcc atcccatgct gctggtagcg atccatcact
    15421 ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag gacgtttgtc catgccgata
    15481 cctgtcgcaa caacggttac gcgcagctcg tcattcatat ccgggtcaag agaagtaccg
    15541 ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg tgttacctac cgtttcgaac
    15601 tcatccagac gcaggtcgaa gcccgccgtg atgttaacca gcacgccgcg cgcgccagac
    15661 aggtcgatat cttccagcag cggagaagag atagccattt cagcagcttc ttccgcacgg
    15721 tcttcaccgc tcgccacgcc agaacccatc attgcgtagc ccatctcaga cattacggtg
    15781 cgtacgtctg caaagtccac gttcatcaaa cccggacgag taatcagttc agcgatacct
    15841 tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg catccagcag ggagataccg
    15901 cggcccagaa ctttcagcag tttgtcgttc gggatagtga tcagagagtc cacatgcttg
    15961 gacagttcag tgatcccctg ctccgcgaat gccatacgct tcttgccttc aaagttgaaa
    16021 ggcttagtga cgacagcaac ggtcaggata cccaaatctt ttgccacttc agcgacgact
    16081 ggtgctgcac ctgtaccggt accaccaccc atacccgcag caataaagac catgtctgca
    16141 ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag ccgcattgcg gccaacttct
    16201 ggattagcgc cagcgcccag tcctttggtg ataccgctac cgatttgaat cgtctgtcca
    16261 accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg cgaagaattc aacaccttca
    16321 atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc cgccgccgac gccgatgact
    16381 ttaatcaccg cgtcattggt aagttccatt ggttcaaaca tagtttctct ccgatttgtg
    16441 cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc
    16501 caactattga gtcgcttgat ccacgagcca actgatgctg taacacgttt ttctacttca
    16561 gcttcaccgt taagatgtga ctctttccca tagtgaagca atcccaccgc cgtcgaataa
    16621 tacggctcct gagcataatc cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact
    16681 tgcgtatgaa acacgcgctg agcacaggct gcaagacctt cgatctgcgc tgcgccaccg
    16741 gttaatacaa tgcctgccgc caggtgatgt ttaacccctt gttggcgaag cttttcctgc
    16801 aactgcaata tctcttcgtt gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc
    16861 tctgccaggg tctgacgttg cagactccgt ggcggacgac cgcctacgct cggcacttcc
    16921 acgctctcat cttttccaac gatggaaccc agcgcacaac cgtggcgaac tttaatcgct
    16981 tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat cactggtcac gacattgcca
    17041 gcataaggaa ttaccttagt gtggcgcaat gccccaccgg tataaacggc gatatccatt
    17101 gtaccaccac cgatatcgac gacgcagaca cccagttcac gttcatcttc cgtcaatacc
    17161 gaataacttg atgccagtcc ggcaaatatc agttggtcaa ctttcagccc acaacgttca
    17221 accgctttga cgatgttttt cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc
    17281 tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc cttcctgata gtcaatcgca
    17341 tactcttgcg ggatcacatg cagcacacga tgttcatcgc gcacacgcac cgatttcgcg
    17401 gtatggacga cgttttccac atcttcttgc gtcacttctt cttcagaaat aggcaccata
    17461 ccaatttcat tctggcagct gatgtgctta ccagaaagcg ccagatatac cgaagagatc
    17521 tgacaatctg ccatcaattc tgcctggtca atggcgcgtt gtacgcactt gaccacggat
    17581 tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg ggcagctgcc cacgccaatg
    17641 atattgacca taccgtcggg cagaacttcc cctactaaag cggcaacctt cgcggtacca
    17701 atctccagtc ctactaccag ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg
    17761 cctgattttg ttgctgagta gattcctctg gcggcaaggg cgcccagcct actgccgctc
    17821 cagagtcata acgcaaatca acgtagctaa tccgtttgcc atcggtttgc gcctgctgct
    17881 gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt catcgtatcg ccccggccaa
    17941 gattgagctt aatatcgtta ttcagcgtca actgccagga acgccgcgcg gtcatcgccg
    18001 cttccttcag agtaaatctg tcctttgcca gcatctgccc catttcgcga tagccctgca
    18061 acacttcatt ggcgctgcct tccgggccat acagcattgg aagcacctgc ttgctggtgc
    18121 gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac catgtgttga tcattccacc
    18181 gcgcaatcgg cacatattca accagatgaa tcttcaattc atcaggccac tgctttctga
    18241 cgctcacctg cttaatccac ggcaggcgtt gttctatttg cgtctggatg atgttgacat
    18301 cctgggtcat aaaggtaccc ggctcaccca atgccaggat cgactgccgg atatcgtcat
    18361 tacgcgtgta atggcgttca ccggtcaaca ccagctttga gagcggcagg cgttgcgcat
    18421 cttccatcca gcccaacacg acccagccgc tcaccaacac tgtcgttaaa acggtcagca
    18481 ggaaaaggat ccccgccaga cgcgttccat tattgcggcg agaagaaacc tcttcttcgc
    18541 tatttcgcgt gttcagagca gcctgcgaca tattagtccg ccagttccag aattcgtact
    18601 accaactgcg agaagctcat acctgcctga cgtgccgcca tcggcaccag gctgtggctg
    18661 gtcatacccg gtgaggtatt ggcttccagc agataaaact gtccatcgct gtccagcata
    18721 acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc atgctttcag cactaatgcc
    18781 tgcaaattgg cctcttgtga cgcttccaga cctgcggggc agaaatactg tgtctcatca
    18841 gagagatact tcgcctcata atcatagaag gttccggacg gttgaatacg tattgacggt
    18901 aaaatttctt caccgagtat cgcaaccgtg aactccggcc cacttagcca tttttcaatc
    18961 aatacttctt catcgtgctg aaatgccaat cttaatgcat cttgtagagc attttctgct
    19021 actacttttg acattcccac actggaacct tcgcggctcg gcttaacgat aaccggcaaa
    19081 cccagagcag aaatttctgc taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg
    19141 gttaacgcta cccacggcgc gaccggtaaa ccggcacctt gccacagcag tttgctgcgt
    19201 agtttatcca ttgaaagcgc agatgccatc actccgctgc cggtataagg caagcccacc
    19261 agctcgagca tgccctgcag cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac
    19321 actttctgaa aacccatcga cttcagttgc gtcacgtcga cttctttcgg gtcgacagga
    19381 tacgcatcaa taccgccttc acgcagcccg gctaacaccg ctgcgccaga attcagagaa
    19441 acttcccgct cagcggaagt cccgcccaac aggaccgcga ttttatcagt catgttgttc
    19501 ttcctccgga gtttgcggct tcagtttgat ttcagctaaa gaacgggcaa tttttccgat
    19561 attaccagcc ccctgaacga gaatcaggtc gttaccggtt aataccggtg ccagcatctc
    19621 ggctacctgc gcagggtccg gcaccagaat gggatcaatt ttcccacgtc cacgaattgt
    19681 gcgacacagc gaacggctgt ccgctcccgg aattggcgct tcgccagccg gatacacttc
    19741 cagcatcaac agggtatcaa cctgcgtcag cacattggcg aaatcatcat acaggtcgcg
    19801 cgtacgggta aaacggtgcg gctgaaacag cattaccagg tttttatccg gccagcctgc
    19861 gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag
    19921 cattgccgta ccgcttttac cattcactgg ctccagcggg aattcaccga ggaaatcaaa
    19981 acggcgccca gtcccctgga agctctccag cgcacgtaaa atagcctcgt catcaattcc
    20041 ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc gcgttatgac gacctggcgc
    20101 attcagggtg acgcgcatcg gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg
    20161 gccaatctgc tgataatctt ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt
    20221 ggtctgacgt cccactcgcg gtaacaattc gcggatcacc ggatcatcaa cacacatcac
    20281 cgcacgaccg taaaacggca ggttgtgcag aaaattaata aaagtctgtt ttaaattctc
    20341 aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat
    20401 cggttgcaga tgcaggaacg atgcatcact ctcatctgct tcggcaatca ggtaccgacc
    20461 atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg
    20521 gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg
    20581 cgttcctgca atggcgatgc catgacgaaa acgcattaac tcagccagca tttcggcacg
    20641 acggatcacc ggaatacgcg cttcatgagc tgcgacaatt tccgggttat cggcagaaat
    20701 ggcgctggaa acaacgacca cgctggcatc acgtacgttt tccgggcgat ggttgaaata
    20761 aatcgtcgct cccagattca ttaactgctg cgtgaccgga tttggcgcta aatcggaacc
    20821 actgatctga taaccttcat tggccagaac ttcggcaata ccgcccatac cggcaccacc
    20881 gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt
    20941 tgccaattgt tgtgtattca ttctttacgc cattaacttc ttaaattcat acgatgcaaa
    21001 aggcatcgct acaattacgc ccgggcagcc cggctcactt catttgccac tcgctcggtg
    21061 gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac
    21121 cacccggcca gggtgttggc gacagcatcc acgctaagct gtggctgctc gataattttg
    21181 gctgcgcccg ctttttccag cggtagcgca ttccagtatt gctggcggtc tttatgttga
    21241 aacggcacaa acaacgccgg tagtcctgcc gcggcgattt cactcaccgt taaagcaccg
    21301 gagcggcaaa cgacgacatc cgcccacgca tacgccgccg ccatatcatc aataaattcc
    21361 gtcactttat gctgcggttg ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa
    21421 cctttaccgc tctgatgcca gatagtgact gaatcaccca gtttcgcagc aacctgcggc
    21481 attgtctggt taagaatgcg tgcgccctgg gaaccaccca ctaccagcac acgaaccgga
    21541 ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc
    21601 gggttaccca ccacttccgc attagggaaa gcgcctggaa acgcctgcat cactttggtg
    21661 gcaatcttcg ccagccattt attggttaag cccgcaatac cgttttgttc atgaagtaca
    21721 accggaatgc ctaacgacca cgcggccaga ccacctggac ctgatacata gccgcccata
    21781 ccgagtacca cgtcaggttt gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg
    21841 aagatacgca gcggcgcagc tatcagtgct tttatacctt ttccacgcag accagagata
    21901 cgaatgaaat caatttcgat gccatgtttt ggcactaagt ccgcttccat acggtcggca
    21961 gtccccagcc agcgaacttg ccaaccctga gccattagat ggtgcgcaac cgccagtccc
    22021 gggaatacat gtccaccggt tccgcctgcc atcaccatta atcgctttcc ttgaccactc
    22081 atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg cgtttcataa tcaatacgca
    22141 acagcatcat gatggctgtc gacataatca gtaagctcga accaccgtaa ctgatcagcg
    22201 gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc gcctacgtta accagcgcct
    22261 ggaagctaaa ccagatgcca atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt
    22321 ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg
    22381 ccagcaccac accgacatac cccagttctt cgccgataat ggcgaaaata aagtcagtgt
    22441 gcgcttccgg cagatactcc agtttttgta ccgagttacc taaaccttgt ccccaaagtt
    22501 cgccgcgacc aaacgccatc agcgattgcg ttaactgata gccgctgcca aagggatctt
    22561 cccacgggtt ccagaatgcg gtaacacggc ggatacggta cggttcggcg agtatcagca
    22621 acacaaccgc tgaaatgccc ataccgataa tggcaatgaa ctgccacaat ttcgctcccg
    22681 ccaggaacaa catcgccagc gtagtcacaa acaacaccac caccgtacca aggtctggct
    22741 gtgccagcag taacactgcc aacaccagaa tcacgcccat cggtttcagg aagccgcgca
    22801 ggttattacg tacttcgtcg cctttacgca ccagatagtt ggcgatataa caaaacagcg
    22861 acagttttgt cagctccgca ggctggatac gcagcaaacc gagatcgatc caacgcgatg
    22921 cccctttaac cgagctaccc actaccagca cgatcatcag caggatgata gatccgagca
    22981 gcatcgtggc actgtagcgt tgccagaact ccatcggcag acgcagcgta atgatcgcca
    23041 gaatgaatgc caggatcaga tagacgccat cacgcttcgc gaagaagaac ggatcgttgg
    23101 ttaagcgttg ccctatgggc attgacgccg aggtcaccat gataaagcca atcgccgcaa
    23161 ggccgaaggt cagccacagt aaggtgcgat cgtacatgat caggctgtcg gtatcttttt
    23221 cccgcgagcc catcacccag ccttttagcg ccgtggagat ccagaccagg atactgaatc
    23281 ctggcaggcg cggcattttc aggcgaggga gagataaacg catcaaccta actccttcgc
    23341 cagacgggca aactcattgc ctcgttgttc aaagttcttg aactgatcaa ggctggcaca
    23401 ggccggggag agcagaacca tatcgcccgg ctgaacacgc ggagccagca agcgcatcgc
    23461 ctgttccata gtttcggttt gttctgccac ttccgggcgt agcgccgcca gctgcgcacc
    23521 gtcacgacca aaacagtaca aacgcacgtt gtcgccattc agatagcgcg ccagtggact
    23581 gaagtccgcc gatttaccat cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa
    23641 gccattcaac gccgcttccg tactgccgac gttagtggct ttcgaatcgt taatccagcg
    23701 tacgccattg tgctccagca ccacttcaaa gcgatgcggc agaccggtga aggtggttaa
    23761 cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct gccagtgcca gtgctgccag
    23821 cgcattggtg tagttatgct gcccggaaag tttcatctct ttcacgttca gcactttctc
    23881 gcctttcact cgcagccagg tttctccctg ctgatgattc aggtgatagt cacccatgtt
    23941 gacgccaaag ctgacgcagc gttcatcagc accgcgaatc ggcattgtta aggcatcatc
    24001 agcattgacg acacaaactt tcgcattttc gtaaatacgc aattttgccg cacggtattg
    24061 ctgcaaacca aacggatagc ggtccatatg atcttcagtc acgttcagaa tggtcgccgc
    24121 caccgcctgt aagctggaag tggtttccag ctggaagctc gacagttcca gcacgtacag
    24181 ttcgcactca gcatcaagca gcatcaacgc tggcaggcca atattgccac ccacaccaac
    24241 gttaaccccc gccgctttcg ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt
    24301 agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg cagaacagct cgatatcgcc
    24361 aacgatttcg attccggcat cagcggcagc gcttaaggat ggatgcgcca gtgcaatacc
    24421 gggactggcg acaatcagat ctgccgccat cagccattca tcattcagac tgcccgtgtg
    24481 gcgttctacg gcttcgggta atttatccag gccaggcggt gtcatacgcg tatccataac
    24541 gcgcggcgtc agaccgcgag cgaggaaaaa gtccacgcag gaaagcccag tgaggcccag
    24601 gccgataatg acgacatttt taccctgata atcagccatg attaacgtac cttcagcgtt
    24661 gccagaccaa tcagaaccag catcagcgaa ataatccaga aacgcacaat gacgcgcggt
    24721 tccggccagc ctttcagttc atagtggtga tgaatcggtg ccatgcggaa aatacgttgt
    24781 ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa gcgtttctac cacgaacacg
    24841 ccccccataa tcaccagcag gaattcctga cgtagcagta cggcgataat gcctaacgca
    24901 ccacctaacg ccagcgaacc tacatcgccc ataaagacct gcgccggata ggtgttaaac
    24961 cacaggaagc ccagtcctgc cccgactatc gcggtacaga caataaccag ttcccctgcg
    25021 tgtcgcagat acggtatatg caagtagctg gcaaagttca tattgccggt cgcccacgcc
    25081 accagcgcaa aaccaccggc gacaaatacg gtcggcataa ttgccaggcc gtcgagacca
    25141 tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga agtaagccag cagaatgtag
    25201 aacagcccca gctgcggcat cacatcttta aagaatggga ccaccagctg cgttgcgggc
    25261 gtgtctttgc cggcaaggta cagggcgaag gcgacaccca gcgcaatgac cgacatccag
    25321 aaatacttcc aacgagcgat caaccctttg gtgtctttac gcaccacttt gcgataatca
    25381 tcaacaaagc caataacacc gtaacctacc agcaccacca acacgcacca gacgtacgga
    25441 ttggacgggt aagcccacag cagtacggag atcacaatcg ccgtcaggat cataatcccg
    25501 cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc
    25561 tgaccaaagg aaagtttttg caaatgagca atcatacgcg ggcccatcca caatgagatg
    25621 aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca gataggaaaa gacgttaaag
    25681 ccggaataat atttgaccaa atgttcggcc agccaaacta acatgtccca ttctcctgta
    25741 aagcgcgtac tacctcttcc atggcggcac tacgtgaacc cttaactaaa atcgtaatta
    25801 cctgttgctc agcaatcagt gatttaagac gcgtaattaa cgcagtttta tcagcaaaat
    25861 gttcgccaac gccgctggcg gtgctgatag catggctttg tttacccacg cttaacacgc
    25921 ggtcaatacc agcagctttt gccgcctcgc ccacctgtac atggcaggct tcgctttcag
    25981 cgcccagttc cgccatatcg cccaccacca gcacgcggta gcccggcatt tcagccagta
    26041 cctgaacagc ggcggtcatt gaaccgacat tggcgttgta ggagtcgtcg agcagcaact
    26101 ggttttctgc cagttggatg gggaacagac ggcctggaac agctttcaga tttgccagcc
    26161 ccgctttgat agcatcaagc gttgcgccca cggacatgga gagcgcagcg gctgccagcg
    26221 cattcgcaat attgtgacgc cccggcaacg gcagcagaac atcgacgcta ccggttgggg
    26281 tttgtagggt aaattccgta ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc
    26341 tgttggcggc attgggtgag aaacgccaca ctttgcgtga gccaattacg ctctgccagt
    26401 tcagccagtc gttgttgtcg gcgttcataa tggcgatacc gttttccggc aggccgctaa
    26461 agatttcacc tttcgctttc gcgacacccg caagcgagcc aaaaccttcc agatgcgccg
    26521 ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag actcacagtc caggctattt
    26581 cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc gtattccggc gttaagcgca
    26641 acagcgtcat cggtacaccg atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc
    26701 cgcactggct taaaatcgcc gccgtcatct ctttaacgga ggttttgccg gaggaccccg
    26761 tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg
    26821 ccagacgcgt atccttgacg attaactgcg gcaggtcgat gtccagcgga cggctaacca
    26881 gtagtgcgcc tgcgccgcca gctttcgcct ggtcggcaaa atcgtgggca tcaaaacgtt
    26941 cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt tcgggtatca gtggttacag
    27001 catcaagggt gatatctgca ccttgcagtt caccgttgag aatgtcggta agttggctaa
    27061 gggttacgct aatcatgcaa tcacccccag cagacgcgcc accgtgacgc gatcggagta
    27121 gtccagacgc tgattgccaa caatctggta atcttcatgg cctttgcccg cgaccagtac
    27181 cacatcattc tctttagcct gcataacggc gcaagtcacc gcttcagcac ggccttccat
    27241 cactttggca tgtccggcat ctaacattcc cgccagaata tcgttgatga tggcacgcgg
    27301 ttcttcggta cgcgggttat cgtccgtcac caccgccacg tcagcaaact cttcggcaat
    27361 tgcgcccatc agtggacgct tacctttatc gcgatcgcca ccacagccaa agacgcacca
    27421 cagcttgccc gcacagtgca ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt
    27481 atgcgcgtaa tccaccacca ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc
    27541 gcaaaccggt tgcagacgcg cggcggtttt cagcagatca gccagtggat agccgagtgc
    27601 cagcagtgtc gccagcgcga gcagcaggtt gctgacgtta aaagcgccca tcagatggct
    27661 ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata
    27721 gttcacgtcg atcgctttta accagcgtcc gtgacagttc ggattgatat gatcttccat
    27781 tgataccgca accgcgtccg gcagttttgc cagccagcgg cggcccactt catcgtcggc
    27841 gttaataatc gcctgaccgc aatgatgttc agaataaagc agccatttcg cagcttcgta
    27901 gtgttccata tcaccatgat aatcaaggtg atcgcggctt aagttggtaa agaccgacgc
    27961 cgcaaatttc aatgccgcca cacggtgctg taccagcccg tgggaggaaa cttccattgc
    28021 gcaaaacgtc gcgccctgct ccaccagccc cgccagctca tgctgaacat cgactgcgga
    28081 accggtagta ttttctgtcg ggatcacttt cccaagcaga ccgttaccaa cggtgcccat
    28141 taccgcgctg gtttcgccaa gcagttggct ccactgcgcc agcagctggg tagtcgtggt
    28201 tttgccgttg gtgcccgtta cgcctacgag acgtaaattg tcagagggtt catggtaaaa
    28261 gcggcccgcc agtgcagata aacgctcgtt gagctggctg agatagatga ccggtacgcc
    28321 gtgcatttca cggatttcac catcggtcgc ctcatctttc gcctctgcaa taatggcagc
    28381 cacaccttgc gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac
    28441 agctacaaag agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc
    28501 tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac
    28561 ctgtcccctc gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg
    28621 gctcgatgtt catggtacgc aatacgccgc ccatgatggc accaaagacc ggcgcggaaa
    28681 cggcgccgcc gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc
    28741 gcggctgact cgcaggcgca acgcctgcgg tataagcaat atatttattg atgtagcgac
    28801 cgtccggccc gacctttttc gcggtaccgg ttttaatggc gatacgatag cctttaatcg
    28861 ccgctttcac gccgccgccg cctggtagcg ccacgctttc catcatatgc accacggtgc
    28921 gaacaatgga ttccgggaag acacgttcac cgggaaccgg ggggtcaact ttggtaatcg
    28981 acagtgggcg ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg
    29041 gtgttaccat tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt
    29101 gtttttgagg atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca
    29161 gtccaaaacg tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagcttgg
    29221 aaacaccgac gttactcgac ttctgtaata ccccggtcag ggttaattcg ctgtagcgtg
    29281 ccacgtcttt gatttcgtgg ccgttaattc gataaggaat ggtattgagt accgagtttt
    29341 cccgcaccac gccacgttgc aacgcggtca ttaccaccat cggtttaacc gttgagcccg
    29401 gttcaaacac gtcggtgatg gtacggttac gcatcgcctc tttcggcgtg ccgctcagat
    29461 tgttagggtt gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca
    29521 ccagcacggc gctaccggat tcagccttgt taaaggccac cgcgttgttc agttcgcgat
    29581 aaaccagcgc ctgcaagcgt tcatcaatac tcaacgccag gttgtgcgcc gcctggctgt
    29641 cagtagaaga aatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac
    29701 ccggctgccc ggtaagccat ttatcgaaac tcttctcaac gccctcaatc ccttgactat
    29761 cgacgttagt aaagccgatg aggtgagcag tcacttcgcc ggacggatag taacggcgag
    29821 actcttcacg cagatgaatc cccggcagtt tcagtttttt gatgtagtcc gccatgtcag
    29881 ggttcacctg acgcgccaga taaataaagc gccctttcgg gttagcgtta atgcgggctg
    29941 aaagctgatc cagcggaata ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc
    30001 tgataccgcc agcgtcatgc acttctttcg ggtcagccca aatcgctttt accggcacgc
    30061 tcaccgctaa cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct
    30121 gaacgcgaag agaacgcatg tcgccctctt tcaccagcat atccggggag ataacttgta
    30181 accacgctac gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg
    30241 caaaacgcca actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg
    30301 ccgctgcttt catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg
    30361 gatcaacatg ctgcatttgc agcttttccg tggcgatcct ttccacccgg ctatggtcgc
    30421 cgagcgcatt ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca
    30481 gcaccagttg ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg gttaccacag
    30541 tcaccgccgt caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa
    30601 gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa
    30661 ctttgcttag agcttctgtc actctgctga tcatgcattc gtcctctctg caatacgcag
    30721 aactgaacta cgggcacgag ggttctcagc cacctcttct tcgcccggca ttaacttgcc
    30781 tagtgctcgc agctgacggc cacccagttt tttgagctgc tcttcagtca tcggtaaccc
    30841 tgccggaact tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tacggtcttc
    30901 cagcgagtgg aagctgatga tcgaaagccg cccacccggg gccagcacgt tgagcgagct
    30961 ttttagcgcc tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa
    31021 ggtacgggtc gcgggatgtt taaacttatc tttcaccggc gttgcagcag ccacgacttc
    31081 cgccagttct ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc
    31141 aatgcgtttg gcaaaacgct cttcaccata ggttttcaat acccaggcga tatcggcttc
    31201 ttctgcggtt tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc
    31261 cagcggacca tcgcgcataa aggaaaagcc gcgctcagca tcatcaagtt gcggtgaaga
    31321 gacgccaaga tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta
    31381 ttcgcccagc gcggagaaag gtccgtggat gatggagaag cgcggatcat caatagtctt
    31441 cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag
    31501 ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc
    31561 atcaggacgg atattgaggc cattaacggc ttcatccagc agcaccgtag tatgtttata
    31621 gttttccatc attttataga gacaagtcct gcagtcgctc cgataagtct ccggtagcca
    31681 actgctctgc gtcgatatct tccttgacct gttgatgcca ggttgtttca tcccacagct
    31741 caaacttgtt gaactgtcca accagcatca cttcttttgt cagcccggca tgttgccgca
    31801 gtactggcgc gattaacaat cgacctgcgc catccatctg acattcgctg gcatgaccta
    31861 acagtaggcg ctgcacacgg cgctcaaccg ggttcatgct cgacagacgc gataattttt
    31921 gctcgataat ttcccattca ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa
    31981 tggtgcaaac catttgaccg gcagcgttct caagcagctg ttcccggtaa cgggtaggca
    32041 ctgataagcg ccctttgctg tcgagattga ctaacgttgc tccccggaac atgccagcct
    32101 caccccttat tcccactttg ccccacaatt tcccactgaa aggagtttac ggagcggagg
    32161 aaaagcttgt caagccagtc acaaggcata ccgaggtaaa aaacccgatg tttacggcta
    32221 atatcagcgt tgtttatttt acgtccaacg tgcgcagcaa attaacgtta tgaatagctg
    32281 taagaaaaaa tccctcatcg tcatcaccgt tttaaaatga ctcactattt tccacagcaa
    32341 atataaagtg tcagtttgcg acgcgagcgg cattttagga catatcttcc ccggttaaca
    32401 gtccctgttg cgtctgtgtg gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag
    32461 tgcttgttaa atcggcaatt catctctgca tgtaacaaaa taatgcaaaa acgcgctgtc
    32521 gatatcactt aattattgtg aatttcagct ggtgatttaa ttaacgggcg ggtttaaaag
    32581 aacttatcaa taatagtcag gaatagtctt atttacttta aggatattga tgtccattcc
    32641 cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc ggcttagcta
    32701 cggctgagca caccgcgacg atagagatta cgtttaatgc gcgttaaacc aggttttggc
    32761 ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg
    32821 tgacgttgag ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca
    32881 aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt tacatccatc
    32941 accccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc
    33001 agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc atacaggaaa
    33061 tgcacttcgc gcggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg
    33121 acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa cttacgtaac
    33181 tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt gaagtgttca
    33241 cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa
    33301 aaaggatgct caggaggcaa cgacgtcgaa acaataatgg catcgacctg acgctgtaaa
    33361 aggtgctcaa tacagcgcat ttcgttgtct ggctgatctt ctgagcaggc aattagcagt
    33421 tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt atagctggtg
    33481 ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct
    33541 gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt
    33601 ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc
    33661 gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt
    33721 aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag cgcagaaaat
    33781 taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt
    33841 gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt ttatcgccgc
    33901 gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca tcgcgaatcg
    33961 atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg
    34021 gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact tcgtcacgcc
    34081 cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc gcgccctgcc
    34141 ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg atctgctcaa
    34201 gcactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga tcgtcggttg
    34261 gcgcaacggt caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg
    34321 ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct
    34381 ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac
    34441 gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc gcacctgttc
    34501 cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga tcccgacgtg
    34561 accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag attgtgaatg
    34621 acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc ggttattgag
    34681 attcaccacc agtaccggca actcgtattg caacgcggta gacagttcct ggatgttcat
    34741 ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg ccattttgac
    34801 gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg ccaccggaat tgatccagcg
    34861 acgcggtttg tcgaatggat aataaagtgc agcaaacatc tggtgctgcc cgacatcgga
    34921 cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt
    34981 aatcttttca ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatctg
    35041 ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg acaggagttc
    35101 aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatcag cagtcacggt
    35161 tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg ggcagtactt
    35221 tgccagatta ttcgtcgttc ggtcatcaaa tcttaccccg acggcgaaaa tcacatccgc
    35281 gttatgcatc gtcatattgg cttcgtaggt gccgtgcatt cccagcatgc ccagcgcctg
    35341 acgatgcgtt gccggaaatg cccccaggcc catcaatgag gaaacaacgg gcagattcaa
    35401 cgcctccacc gtttctttca actgctgatg gcagcccgcc gtgattgccc caccgcctac
    35461 gtagacaacc ggtttttttg ccgccaccag cgtttgcaga gcacgcttaa tttgcccttt
    35521 atgtccggta gtagtgggat tgtaagaacg catactaacc gactccggcc agacataggg
    35581 taatttgttc gccggattaa gaatatcttt cggtaaatca acgactactg gtccaggacg
    35641 accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt
    35701 aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc actcctgaaa
    35761 ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga
    35821 atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac ccgacgttac
    35881 cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat gcaccgccgc
    35941 ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg catcgtaaat
    36001 atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga
    36061 tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa
    36121 aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag ccagagacat
    36181 aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca
    36241 ttacgaagga ttaaattgac aataaatcgg ctgaatccca caacttacag caatttctct
    36301 atttttttaa ttaatcctaa aatagagagc acacacattc agaataaaat tctacattca
    36361 ataaaaaata cagaataaac cagacattca tgtctggcct attctgcatt cagttagcgt
    36421 ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg cccggcagct
    36481 tcatgccagg agagataaca ggttctgctg ttttgtttta acggcagcgg taatacctgt
    36541 aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc
    36601 gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct
    36661 tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc
    36721 gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttggatga
    36781 tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga
    36841 cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca
    36901 gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg
    36961 tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca aagatgaaat
    37021 acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc
    37081 aatgcctgac gaactgagcc aaaaagttga aatgcgcgag cagtcggttg aataccacgg
    37141 ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg
    37201 accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg ctcctgcatc
    37261 acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgcttaac
    37321 tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt
    37381 aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga tttatcatgc
    37441 atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat acgggttctg
    37501 ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat aatccataat
    37561 gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca tctacaaaat
    37621 ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat tcttaacatt
    37681 aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca tataattcaa
    37741 tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt cctcgttgat
    37801 tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt agttaagcag
    37861 aaattaatat cgcttacttt aaccaccgca gcacaattag ctaattttac ggatgcagaa
    37921 ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg tatccagtaa
    37981 ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg ttcgctttat
    38041 cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg gcatccagca
    38101 ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt tttatgcccg
    38161 aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt cattattttc
    38221 gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa
    38281 ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc
    38341 gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt taaaaacagc
    38401 cgcgtatgtg cgttagctcg ctgcgtggag aaagatatcg acgtggcggc cgaatccctg
    38461 aaagtcgccg aagccttccg tattcatacc tttattgcca cttcgccaat gcacatcgcc
    38521 accaagctgc gcagcacgct ggatgaagtg atcgaacgcg ctatctatat ggtgaaacgc
    38581 gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg tacacccatt
    38641 gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac catcaacatt
    38701 ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag cggcctgtat
    38761 gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga cgatttaggc
    38821 ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt ggaaggtgca
    38881 atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat catggcgatt
    38941 aaagttcgta aggatattct caacgtccac accgccatta atcaccagga gatatggcgc
    39001 accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa agccattgtt
    39061 ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct gaaaaaccgc
    39121 gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca gctgaatctg
    39181 acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg gtataaagaa
    39241 agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga caaaaaaggt
    39301 caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca agaagagccg
    39361 gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat cgccaccgcc
    39421 gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg taacggtccg
    39481 gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga actggtgaaa
    39541 tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc
    39601 aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc
    39661 gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt cgaaaaagag
    39721 ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt
    39781 accatattgc cgtattgccg ggggacggta ttggtccgga agtgatgacc caggcgctga
    39841 aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc cattacgatg
    39901 taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg gttgaaggtt
    39961 gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg tccgaaatgg gaacatttac
    40021 caccagacca gcaaccagaa cgcggcgcgc tgttgccttt gcgtaagcac ttcaaattat
    40081 tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt ccgctgcgtg
    40141 cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg cgaactgacc ggcggcatct
    40201 atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg tttgataccg
    40261 aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa tctgctcgta
    40321 aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc tctattttat
    40381 ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg gcgcatatgt
    40441 acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac gttctgctgt
    40501 gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact ggctcgatgg
    40561 ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg tctgtatgaa ccggcaggcg
    40621 gctcagcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcgcaa attctgtcgc
    40681 tggcactgct gctgcgctac agcctggatg ccgatgatgc ggcttgcgcc attgaacgcg
    40741 ccattaaccg cgcattagaa gaaggcattc gcaccgggga tttagcccgt ggcgctgccg
    40801 ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat
    40861 catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa
    40921 cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct caccgcaggc
    40981 gtttgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac
    41041 tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg aaatggcgcg
    41101 catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc tgtatgacct
    41161 gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg tcaccttgcc
    41221 ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt ttggcgcact
    41281 ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa ccctgaaaca
    41341 gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg gcattacagc
    41401 aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca ccgggcatgt
    41461 ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta tgaccctgtg
    41521 caatatggca atcgaaatgg gcgcaaaagc cggtctggtt gcaccggacg aaaccacctt
    41581 taactatgtc aaaggccgtt tgcatgcgcc gaaaggcaaa gatttcgacg acgccgttgc
    41641 ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg tcactctgca
    41701 agcagaagaa atttcaccgc aggtcacctg gggcaccaat ccaggccagg tgatttccgt
    41761 gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgcg cgtcggcaga
    41821 aaaagcgctg gcctatatgg ggctgaaacc gggcattccg ctgaccgaag tggctatcga
    41881 caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg cggcagcgga
    41941 gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc ccggctctgg
    42001 cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atctttattg aagccggttt
    42061 tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc gtctgaatcc
    42121 gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg
    42181 gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg gacatttcgc
    42241 cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc aaacacacag
    42301 gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc ccgaaacagt
    42361 ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac tggcgttttc
    42421 tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg cagtatcagg
    42481 gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt gagcacgcgc
    42541 cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt gctgacatct
    42601 tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat gcagaagtgg
    42661 acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg gatctggaag
    42721 cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc ttccgccgcc
    42781 actgcatgat gaacggtctg gacagtattg ggcttacctt gcagcacgac gacgccattg
    42841 ccgcttatga agcaaaacaa cctgcgttta tgaattaatc cccttgcccg gtcaaatgac
    42901 cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt taaaaataat
    42961 gcgacaacag aaataaccgc aattacccag tagacagcaa agtgccccca actttgtgca
    43021 attgctccct gaataacgcc agccagaatt accccggtag aaatactgtt agtaaataag
    43081 gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat cccaataccc
    43141 gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc catacggcta
    43201 tgaaagaaaa tcaatccggt gtaaaacagt actcctgccg ccactgctat gaccatcatt
    43261 cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg tatttccagt
    43321 ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa ctcgctactg
    43381 atccacaacg gcatatcaat aatgtacatg gtgttgcagg tccacattaa cgtcgaggcg
    43441 acaaataaca tccgtacgtt actatcctgc cagccacctt gcattgataa agcattttcc
    43501 gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac cagactgagt
    43561 gtgaatatcc cggcggcaat cgaaaacatc accgtaaagc cgtaattcaa cgccagcata
    43621 aaggccaacg gtggaccgat aacccatgcc agagaaagct gcgcacgcat caccgagcta
    43681 aacatcacca cttctcgcgc cgagttatcc gcatattccc gcgccagagc aaataactgt
    43741 ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag cgtcagataa
    43801 tgacgattaa atgcaaacaa tagcgcattg ccgatagcca tcaaacagca aaatataatc
    43861 agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct tacgccgatc
    43921 ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc gccaacctca
    43981 cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc catcataaaa
    44041 gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt cattatccag
    44101 atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta taatctgctg
    44161 gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa ctttcagaat
    44221 tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg acgcttaacc
    44281 aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac tgacgcatgg
    44341 ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa taaaattgct
    44401 gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa cagttcatcc
    44461 gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa ctggcagcgt
    44521 tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag gatcgcggct
    44581 ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca ttcctctata
    44641 ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat cgtatcgacc
    44701 aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt tctcatctgg
    44761 gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt ccgttgcgta
    44821 atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg caaatcttca
    44881 gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc gcccaccact
    44941 ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc cattttcatc
    45001 atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc aatacgctgc
    45061 cgctctattc gcatattgcg gacattgtgt cgccgacgcc ctggacgctg gatatccatc
    45121 tcacgcaacc ggaccgctgg ttaccgttac tgctgggaca agttccggcg atgatcctgc
    45181 cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc catcggcacc ggtccgtatg
    45241 cggtgatccg caacaccacc aatcaactga aaattcaggc attcgatgac ttcttcggtt
    45301 accgggcatt aatcgatgaa gttaacgtct gggttctgcc ggaaattgcc gacgagccag
    45361 ctggaggtct gatgctaaaa ggtccacagg gcgaggaaaa agagattgaa agccgcctgg
    45421 aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg aatcagcaag
    45481 tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc gctgaggaac
    45541 agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac catgcccgca
    45601 ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt tatcaggatc
    45661 acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt caccaggtca
    45721 cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc gagagcgata
    45781 tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc gcgcacttgt
    45841 gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac gccgcccgct
    45901 ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc agtaaagcaa
    45961 tggtgccgct tatccaccac tggctgatca ttcaggggca acgcagtatg cgcggcctgc
    46021 gcatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca ccggatccat
    46081 gattgctggt tgataacaaa atcactacac taacgccgtt ctcaacgggg tgccacgcag
    46141 acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc gaagggattt
    46201 gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt aaaaaaatgt
    46261 ctgcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct gactgtttat
    46321 acctacgatt ccttcgccgc caactggggg ccaggcccgg tggtaaaaaa agcctttgaa
    46381 gccgactgta attgcgaact gaaactggtg gcgctggaag atggcgtttc gcttctcaac
    46441 cgtctgcgca tggaaggcaa aaacagtaaa gccgatgtgg tgctggggct ggataacaac
    46501 ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggcgtggc agcggatgcc
    46561 gttaacgttc ccggcggctg gaataatgac actttcgtac cgtttgatta cggctatttc
    46621 gccttcgtct atgacaagaa caaactgaaa aatccgccgc aaagcctgaa agagcttgtt
    46681 gagagcgatc aaaactggcg ggtgatttat caggatccgc gcaccagtac gccggggctg
    46741 ggcctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc ctggcagaaa
    46801 ctggcgaaga aaacggtcac ggtcaccaaa ggctggagcg aagcctacgg cctgttttta
    46861 aaaggtgaaa gcgatctggt gctgagttac accacctctc cggcttatca cattctcgaa
    46921 gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct gcaggtggaa
    46981 gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt cctccagttt
    47041 atggtttctc cggcttttca gaatgcgatc ccaaccggca actggatgta tccggtggca
    47101 aacgtcacgc tgcctgccgg ttttgaacaa ttgaccaaac ccgcaaccac gctggagttc
    47161 acgccagccg aagtggcggc acaacgtcag gcatggatta gcgaatggca acgcgccgtc
    47221 agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg gtggtagcgg
    47281 ttgcgctggc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac tgggtggcaa
    47341 tctggcagga cagctacctg tgtcatgtgg tgcgcttctc cttctggcag gcgtttctct
    47401 cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat cgcaggcgct
    47461 ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc ccggtgctag
    47521 ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca tcgctctgcc
    47581 aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt ttgctggcgc
    47641 acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg gaaaacattc
    47701 ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat tttttccgct
    47761 tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt atctttatgc
    47821 tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag gcaactacta
    47881 tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc gcggcgatgc
    47941 tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag cgactaagta
    48001 aggccattgc gcccggtacc acgctgctgc aaggctggcg cgatccggac gatcgtctgc
    48061 atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg ctgccaccgt
    48121 tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg gcacaaccgg
    48181 tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt gtattgtgcg
    48241 tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg cagaaaatgc
    48301 tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg gggattgtgc
    48361 tggctaccgg cttcttttta ctgctcaaca acaccattgg cctgccgcaa tctgctgacg
    48421 gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa gtactggaaa
    48481 acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg ggcattgaag
    48541 gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg gcgcaggcgc
    48601 tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg ttcggtaacg
    48661 atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat cgcagccagg
    48721 acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt accgtgattg
    48781 aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta tcaccatttg
    48841 ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct cgggccaagc
    48901 ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc agccagcggt
    48961 tcgctgacta tcgacagcgt tgatcacacc acgacgccgc cgtcacgccg cccggtatcg
    49021 atgctgtttc aggagaacaa cctgttcagc cacctgacgg tcgcgcagaa catcgggctg
    49081 gggcttaatc cgggattgaa gctgaacgcg gcgcagcagg agaaaatgca cgctatcgcc
    49141 cgccagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc cggcggtcag
    49201 cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt attgctcgat
    49261 gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct ggtgagcacg
    49321 agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga agatgcggcg
    49381 cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca gggtaagacc
    49441 aatgagttgt tgagcggtaa ggcgagtgct tcggcactat tggggattac gggttagtat
    49501 gcactgtcgg atgcggcgtg aacgccttat ccgacctacg ggtagtgcgt tttgtaggca
    49561 tgataagacg cggtcagcgt cgcatcaggc aacccgcaca agactatctc ctaaccccca
    49621 accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat taacaccacc
    49681 agcgccacca cgccgatggc agaaattaac ggcgtcagcc acaacaaacg gccgcgcgac
    49741 aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca gcacagccag
    49801 ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc gccgctctgc
    49861 attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa gtaaaacggt
    49921 ggccacagca ggcagccaat aatattcggc gtaatgaatt ttgccaccgg cagatccagc
    49981 attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc gaccagaatg
    50041 gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc tttgtttttc
    50101 ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca gaaggagatc
    50161 cagtcgccca gcagacaacc gacaatccct gccagccagg cgtgccagaa acttaactcg
    50221 ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag aatcaaaccg
    50281 accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga atacacggta
    50341 gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa tggacgaagc
    50401 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
    50461 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
    50521 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
    50581 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
    50641 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
    50701 atgtgacaga cgcgacggtg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
    50761 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
    50821 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
    50881 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
    50941 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
    51001 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
    51061 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
    51121 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
    51181 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
    51241 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
    51301 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
    51361 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
    51421 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
    51481 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
    51541 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
    51601 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
    51661 ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt gattctgtgc
    51721 gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta gagtggtatc
    51781 cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt catcatccgc
    51841 gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag cttagcgtcg
    51901 aacagcgcgc agctgtggtc gggattggcg ttgacacaac cggctcgacg cccgcaccga
    51961 ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac ccgaacgcga
    52021 tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt acccgtttgt
    52081 gccacgcgcc gggcaacgtt gactactccc gctacattgg cggtatttat tccagcgaat
    52141 ggttctgggc aaaaatcctg catgtgactc gccaggacac cgccgtggcg caatctgccg
    52201 catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc acccgcccgc
    52261 aggatattcg tcgcggacgt tgcagcgccg ggcataagtc gctatggcac gaaagctggg
    52321 gtggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat cgccatttgc
    52381 cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc ttatgcccgg
    52441 aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc gcgtttgact
    52501 gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa gttatcggta
    52561 cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg gcagtgaaag
    52621 gtatttgcgg tcaggttgat ggcagcgtgg tacctggatt tatcggtctg gaagcaggcc
    52681 aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg ccgctggaac
    52741 agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag aaacaactgc
    52801 ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg ccggtggtgc
    52861 tcgactggtt taacggccgc cgcacaccga acgctaacca acgcctgaaa ggggtgatta
    52921 ccgatcttaa cctcgctacc gacgctccgc tgctgttcgg cggtttgatt gctgccaccg
    52981 cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc gtcaataacg
    53041 tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc tgctgcgacg
    53101 tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc ggtgcggcga
    53161 tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag caaaaaatgg
    53221 ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc tttgaacagc
    53281 tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt ccaacttccg
    53341 ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat gacgattttt
    53401 gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc ggaaaccctg
    53461 cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga agcgaaactg
    53521 ccctgcaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac cgctatttgc
    53581 cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca caccttctcc
    53641 ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct gcaattccac
    53701 acccagttta acgcggcgct gccgtgggac agtatcgata tggactttat gaacctgaac
    53761 cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg tcagcaacat
    53821 gccgtggtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg ctcctggatg
    53881 cgccaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgttt tggcgataac
    53941 atgcgtgaag tagcggtcac cgatggtgat aaagttgccg cacagatcaa gttcggtttc
    54001 tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat cagcgacggc
    54061 gatgttaatg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc tgccacacaa
    54121 atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct ggggatgaag
    54181 cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga tttgcacggt
    54241 ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc agcagggtta cggctttgcg
    54301 ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat gtcaaccggt
    54361 ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa aggtaatgac
    54421 ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga agagaaaccg
    54481 atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg actgatcttc
    54541 aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga tcgttaccgt
    54601 ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa actgccggtg
    54661 gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg cttccgaagc gtggatcctc
    54721 gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga tatgcgccag
    54781 ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg cctgccagcg
    54841 tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta agtagccaca
    54901 tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca cgctgcgatt
    54961 ttgtaggccg gataagcaaa gcgcatccgg cattgttttc cccctcgccc ctttggggag
    55021 agggccgggg tgaggggaaa cgacctctca ggagtcaaca tgttagaaga tctcaaacgc
    55081 caggtattag aagccaacct ggcgctgcca aaacacaacc tggtcacgct cacatggggc
    55141 aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat
    55201 tacagcgtca tgaccgctga cgatatggtc gtggttagca tcgaaaccgg tgaagtggtt
    55261 gaaggtacga aaaagccctc ctccgacacg ccaactcacc ggctgctcta tcaggcattc
    55321 ccctccattg gcggcattgt gcatacgcac tcgcgccacg ccaccatctg ggcgcagtcg
    55381 ggtcagtcga ttccagcaat cggcaccacc cacgccgact atttctacgg caccattccc
    55441 tgcacccgca aaatgaccga cgcagaaatc aacggcgaat atgagtggga aaccggtaac
    55501 gtcatcgtag aaacctttga aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg
    55561 gtccattccc acggcccgtt tgcatggggc aaaaatgccg aagatgcggt gcataacgcc
    55621 atcgtgctgg aagaggtcgc ttatatgggg atattctgcc gtcagttagc gccgcagtta
    55681 ccggatatgc agcaaacgct gctggataaa cactatctgc gtaagcatgg cgcgaaggca
    55741 tattacgggc agtaatgact gtataaaacc acagccaatc aaacgaaacc aggctatact
    55801 caagcctggt tttttgatgg attttcagcg tggcgcaggc aggttttatc ttaacccgac
    55861 actggcggga caccccgcaa gggacagaag tctccttctg gctggcgacg gacaacgggc
    55921 cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt tattcccgcc gatcaggttc
    55981 cccgcgctca gcatattttg cagggtgaac aaggctttcg cctgacaccg ctggcgttaa
    56041 aggattttca ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc caattgatga
    56101 attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc
    56161 cagaacgcta tctgatggag cggtttatca cctcaccggt gtgggtcgag ggtgatatgc
    56221 acaatggcac tatcgttaat gcccgtctga aaccgcatcc cgactatcgt ccgccgctca
    56281 agtgggtttc tatagatatt gaaaccaccc gccacggtga gctgtactgc atcggcctgg
    56341 aaggctgcgg gcagcgcatc gtttatatgc tggggccgga gaatggcgac gcctcctcgc
    56401 ttgatttcga actggaatac gtcgccagcc gcccgctgct gctggaaaaa ctcaacgcct
    56461 ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa cgtggtgcag ttcgatctgc
    56521 gaatgctgca aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg cgcgataata
    56581 gcgagctgga gtggcgcgag cacggcttta aaaacggcgt cttttttgcc caggctaaag
    56641 gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc gttctggaat ttctcttcat
    56701 tctcgctgga aactgtcgct caggagctat taggcgaagg aaaatctatc gataacccgt
    56761 gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt
    56821 ataacctgaa agattgcgag ctggtgacgc agatcttcca caaaactgaa atcatgccat
    56881 ttttactcga acgagcaacg gtgaacggcc tgccggtgga tcggcacggc ggttcggtgg
    56941 cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc
    57001 tcggcgaagt gccaccgcac gccagccctg gcggctacgt gatggattca cggccagggc
    57061 tttatgattc agtgctggtg ctggactata aaagcctgta cccgtcgatc atccgcacct
    57121 ttctgattga tcccgtcggg ctggtggaag gcatggcgca gcctgatcca gagcacagta
    57181 ccgaaggttt tctcgatgcc tggttctcac gagaaaaaca ttgcctgccg gagattgtga
    57241 ctaacatctg gcacgggcgc gatgaagcca aacgccaggg taacaaaccg ctgtcgcagg
    57301 cgctgaaaat catcatgaat gccttttatg gcgtgctcgg caccaccgcc tgccgcttct
    57361 tcgatccgcg gctggcatcg tcgatcacca tgcgtggtca tcagatcatg cggcaaacca
    57421 aagcgttgat tgaagcacag ggctacgacg ttatctacgg cgataccgac tcaacgtttg
    57481 tctggctgaa aggcgcacat tcggaagaag aagcggcgaa aatcggtcgt gcactggtgc
    57541 agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca acggctgacc agcgcattag
    57601 aactggagta tgaaacccat ttctgccgtt ttctgatgcc aaccattcgc ggagccgata
    57661 ccggcagtaa aaagcgttat gccggactga ttcaggaggg cgacaagcag cggatggtgt
    57721 ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag tttcagcagg
    57781 agctatacct gcgtatcttc cgtaacgagc catatcagga atacatccgc gaaaccatcg
    57841 acaaactgat ggcgggtgaa ctggatgcgc ggctggttta ccgtaaacgc cttcgccgtc
    57901 cgctgagcga atatcagcgt aatgttccgc ctcatgtacg cgccgctcgc cttgccgatg
    57961 aagaaaacca aaagcgcggt cgccccttgc aatatcagaa tcgcggcacc attaagtacg
    58021 tatggaccac caacggcccg gagccgctgg actaccaacg ttcaccactg gattacgaac
    58081 actatctgac ccgccagcta caacccgtgg cggagggaat actccctttt attgaggata
    58141 attttgctac acttatgacc gggcaacttg ggctattttg agcaaaaaaa agagttcgcc
    58201 agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat
    58261 tcctggacct gaataacgcc actacctcat aagcacggta gcgggtggtt attgcctgca
    58321 attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga
    58381 tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt
    58441 acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg
    58501 cgtgatgttc aaccctggtg ataccattac cagccatgac ggctggcaga tgcaagtcga
    58561 agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga
    58621 gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca
    58681 ggaccgtctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc
    58741 gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac
    58801 cagcctgatt ccgcatcagc tcaacatcgc tcatgatgtt ggtcgccgcc acgcgccgcg
    58861 cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatcctgca
    58921 tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca
    58981 gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgcgc tatttgatga
    59041 tgagcgttat gccgaagctc agcacgatgc ttacaacccg tttgacaccg aacagctggt
    59101 gatttgctcg ctggattttg cccgtcgtag caaacagcgc ctggaacatc tctgtgaagc
    59161 cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagatgcgcc
    59221 aagccgtgaa tatcaggcca ttgaacaact ggcagagcac gtgccgggcg ttctgctgct
    59281 gaccgcgacc ccggaacagc tggggatgga aagccacttc gcccgtctgc gtctgctgga
    59341 cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt atcgtccggt
    59401 tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat
    59461 gctcggcgag atgatcggcg agcaggatat cgagccgctg ttgcaggcag caaacagcga
    59521 cagcgaagat gcccagagcg cccgtcagga gctggtttcg atgctgatgg atcgccacgg
    59581 caccagccgc gtgctgttcc gtaacacgcg taacggtgtg aaaggattcc cgaaacgcga
    59641 gctgcacacc attaaactgc cgctaccgac gcagtatcag acggctatta aagtctccgg
    59701 cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct acccggagcg
    59761 tatttatcag gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga
    59821 gtggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgccaa
    59881 agctgccact gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta ttcgcgctgc
    59941 ggtgttccac gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga
    60001 agaagacacc ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt
    60061 ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc tactggagca
    60121 gcgtattggt cgtctggatc gtatcggcca ggcgcacgat attcagatcc atgtgcctta
    60181 tctggagaaa accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt
    60241 tgagcacacc tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa
    60301 ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga
    60361 gcaacatgaa gcgctgaaag cacagctgga acagggtcgt gaccgcctgc tggaaatcca
    60421 ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga
    60481 taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca atcaggacga
    60541 tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc
    60601 tggcctgtcg gaagatggca tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga
    60661 agatgcacag tttattacct gggagcatcc gctgatccgc aacggtctgg atctgattct
    60721 ttctggcgat accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg
    60781 tacgctgttg gtggaactga tttatgtggt tgaagcccag gctccgaagc agttgcagct
    60841 caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct
    60901 ggcggcgcag gtagagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac
    60961 cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac gctatccttc aactgggtga
    61021 agcgcagatc gagaaatctg cccgtgcatt gattgatgca gcgcgtaacg aagccgacga
    61081 aaaactgtct gccgagctgt ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg
    61141 tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca
    61201 ggcaggttgg cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg
    61261 aaaatgggga tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag
    61321 gatgaccata ttatggtggt caacaagccg agcggtttgt tgtcagtgcc gggtcgtctg
    61381 gaagagcaca aagacagcgt gatgacgcgc attcagcgtg attatccgca ggcagaatcg
    61441 gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg
    61501 gagcgggagt taaaacgcca gttccgcgag cgcgagccga aaaagcagta tgtggcccgc
    61561 gtctgggggc atccatcccc cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg
    61621 ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat
    61681 gaagtggtgg agtatgcggc ggataacacg gcaagagtgg tgttaaaacc gattaccggg
    61741 cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc atccgattct cggcgatcgt
    61801 ttttatgcat caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg
    61861 ttgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt
    61921 taatcgtcac gctggcgcag ttcggtaggc ggataaggcg ttcacgccgc atccggcaat
    61981 tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca gtttaaaact
    62041 tatttaaacc ctttctgctg ctttatcagc tcatatgcct gctgaatttc ctgcgctttc
    62101 tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg
    62161 tgttcactca tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc
    62221 acgcccagca cattacaggc atcttccagc gttgggccac gctgcgcttg ctgccagtta
    62281 ccaccgccag tttgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc
    62341 aaaaactggt caaactgggc gcgggagatc cctaattctt cagcaatgac atacagcacc
    62401 gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga
    62461 aacatacgaa ttaagtcaaa acgaccaaag cagacactgc gaaactggcg catcttttcg
    62521 cgcagcgggt aattgtctga ttttcccacc cggaacgcat tttgcgccgc agtacgggaa
    62581 gcgccatgaa gattcattcg gtccatcaac tggctggcga tatgaatatc agcctccgtg
    62641 acgcgacctt tggatttggt taaatgcccc atcacttcaa aagtggtggc aaaaaacagc
    62701 gcctgacgct cacgctggtt ggcgaaccac gccattttac ggctacgggc tttatcaaac
    62761 atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc
    62821 acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat
    62881 atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg cggtcaattg
    62941 tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca
    63001 ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga
    63061 ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg
    63121 tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc
    63181 agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca
    63241 gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc
    63301 ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc
    63361 cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgtaccgt
    63421 tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg
    63481 ctgggcgaat ctgaacacca aagataccaa cgtctgggaa ggtgattacc agatggtggg
    63541 tcgccagggt cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc gctataccat
    63601 tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg
    63661 tagcgaaatt attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa
    63721 ggtgggtccg gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg
    63781 tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta
    63841 cctgccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat
    63901 gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc
    63961 tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa
    64021 cgatgacagt tcacgtcgtt ggttattcta ctggaaccac tccggggtca tggatcaggt
    64081 gtggcgtttc aacgtcgact acaccaaggt cagcgatcct agctacttca atgatttcga
    64141 taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc
    64201 ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggttttca gcgaacagaa
    64261 caccagtagc tactcggcag agccgcagtt agacgttaat tactaccaga atgatgttgg
    64321 tccgtttgat acgcgtattt acggccaggc agtgcacttt gttaacacca gagacgacat
    64381 gcctgaagca acccgtgttc acctggaacc gaccatcaat ttgccgctct ctaataactg
    64441 gggcagcatc aataccgaag cgaagttgct ggcaacccat tatcagcaaa ctaatcttga
    64501 ctggtataac tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg taatgccgca
    64561 attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta
    64621 tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg atcagagcga
    64681 catctataac tacgactcat ctctgctgca atctgactac tctggcctgt tccgggaccg
    64741 gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc
    64801 tcgcatatat gatgatgctg ccgttgaacg ttttaatatt tccgttggtc aaatctacta
    64861 tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg
    64921 ctcactggtg tgggcaggcg atacttactg gcgtatctcc gagcgttggg gattgcgtgg
    64981 cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata
    65041 ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc cggaatatat
    65101 tcaggctacg ctgcctaagt actattccac tgctgagcaa tataagaatg gtatttcgca
    65161 ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg tccattgttg gggcctacta
    65221 ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg
    65281 ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca
    65341 acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa
    65401 ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt
    65461 gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga
    65521 agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc
    65581 cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg
    65641 ttgatggatt aatgcagtcg gtaaaactga acgctgctca ggcaaggcag caacttcctg
    65701 atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc
    65761 agatggggca gaaaatggga gtgaaaatct ccgatgagca gctggatcag gcgattgcta
    65821 acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg
    65881 gactgaacta caacacctat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc
    65941 gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc
    66001 agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc
    66061 cgctgccgga aaacccgacc tctgatcagg tgaacgaagc ggaaagccag gcgcgcgcca
    66121 ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct cattctgccg
    66181 accagcaggc gctgaacggc ggccagatgg gctggggccg tattcaggag ttacccggga
    66241 tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggcccg attcgttccg
    66301 gcgttggctt ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg
    66361 tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac
    66421 aggcccgtgt gaaactggaa cagattgctg ctgatatcaa gagtggtaaa acgacttttg
    66481 ctgccgcagc gaaagagttc tcgcaggatc caggttctgc taaccagggc ggcgatctcg
    66541 gttgggcaac agcggacatt tttgatccgg ctttccgtga cgccctgacc cgcctgaaca
    66601 aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg
    66661 atacccgtaa tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac cgcatgctga
    66721 tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgt gccagcgcct
    66781 acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga
    66841 gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga
    66901 actggttgtt tgtgccgatg ccactctcct taccgaccgg gcagcgatgc tcggtttgcc
    66961 gctcaccctc cgcacttatt cccccaactc tcctgcacaa ccgcaaactg cgggcacatt
    67021 aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag cggttgaaaa
    67081 tgggcattat gtggtggaaa cgctggcgcg agcgtgcgat ggctgtctga acggtgaatt
    67141 tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag gcattccgtt
    67201 taccggtcat accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct
    67261 ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc
    67321 agatgctatc acccctgcgc ttttgcacga agtgattgct attttgcatc acgatttgcg
    67381 gaccaaattt ggtattgccg aaccgcgcat tctggtctgc gggctgaatc cacacgcggg
    67441 cgaaggcggt catatgggta cggaagagat agacaccatt attccgctgc tcgacgagtt
    67501 gcgggcgcag gggatgaaac ttaacgggcc gctgcctgcc gataccctgt ttcagccgaa
    67561 atatctcgat aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct
    67621 aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac
    67681 atcagtggac cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg atgtcggcag
    67741 ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga
    67801 gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc
    67861 gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg gccaggcaat ggtcgaaatc
    67921 ggccccggtc tggcggcatt gaccgagccg gtcggcgaac gtctggacca gctgacggtc
    67981 atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa
    68041 ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg
    68101 ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc
    68161 catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg
    68221 gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg gtcgattaag cgtcatggcg
    68281 caatactatt gcaacgtgat cccagtgctg gaagtaccgc cgtcagcctt tacaccacca
    68341 cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt
    68401 aaagatgttc gtgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc
    68461 attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat ggggatcgac
    68521 ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg
    68581 gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc
    68641 aggttcaaag cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg
    68701 cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact
    68761 ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg
    68821 tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa
    68881 ccccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca
    68941 gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg
    69001 cgacatacct tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata
    69061 aagtagaatt tacccctggg aaagataccc tctggctgac gggcgatctg gtcgcgcgcg
    69121 ggccgggttc gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc gtacgtctgg
    69181 tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggatcagc cgcaataaac
    69241 cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc
    69301 tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa gttggtgatg gcccacgcag
    69361 ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg cgcgcgcgat gtagaagcgg
    69421 tgctatcgag tgactcctat cccttctttc tcgacgctat gtacggcgat atgccaaata
    69481 actggtcacc ggaattgcgg gggctgggaa gactgcgttt tatcaccaac gcctttaccc
    69541 gtatgcgttt ttgctttcca aacggtcaac tggatatgta cagcaaagaa tcgccggaag
    69601 aagcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca
    69661 gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg
    69721 cgctggatac cggctgctgc tggggtgggt cattaacctg cctgcgctgg gaagataagc
    69781 agtattttgt ccagccgtcg aaccggcata aggatttggg cgaagcggcg gcgtcttaaa
    69841 cacagcctga tataggaagg ccggataaga cgcgaccggc gtcgcatccg gcactagccg
    69901 taaattctat acaaaattac cgccgctcca gaatctcaaa gcaatagctg tgagagttct
    69961 gcgcatcagc atcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg
    70021 ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttttgcgctt
    70081 ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac catgatttct ggtacgtcac
    70141 cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtacccg
    70201 gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccagg
    70261 tatggcggcc cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg
    70321 caggcaggtt ccacggcatg gcgttttcca tgccgataac gcgatctacc gctaacgccg
    70381 caatcagact gatcattgag atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc
    70441 gtaaaccgtc gtcgactggt gcgaggatga tgttgaggaa aattttatat tctgctggcg
    70501 agtccacgct ctctccctgg actcgccgca ttacaatgaa acaaaaacaa acagttagct
    70561 gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc
    70621 catgttgccg gtatgtttac cttcttcggt tccctgccag ccatgtcgtt gaattaatga
    70681 cagatgttcg cggtcctcgg taatgatctc acttaacatc gcgctggtgc gtttatagac
    70741 cgccgtgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca tctgaatatt
    70801 aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc
    70861 cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac
    70921 gcctgcctga cgcaaacgaa tgtagtggtc gacatcgcgg gcgcgggcaa taatctgcaa
    70981 atgcgggaaa tgttctttca ccatctctgt cagttgcagg ttggtttgcg gatcgtcgat
    71041 ggcgttaatc agcacttccg ctttcgccgc tccggcagat tccagtaaat ccatccgcgt
    71101 ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat ggtccggatc
    71161 gtgatcgagt accaccattt tcaccccgct ggagagcagt aaacgtccgg taatctgccc
    71221 aaaacgaccg aatccggcga taatcacgcg cggctgttct tcgtcgatct catcggcttc
    71281 acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt
    71341 tgctgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac
    71401 attcgccatc tgcgccgcgc caaataccac aaaggcaaac tcactgccct gccctaacaa
    71461 caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa tcagccacag
    71521 catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc
    71581 aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa
    71641 acctttaaat ggttcgatat cgctctccag cgcatgacgg tattccgaac ttgccagcag
    71701 tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc
    71761 aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc
    71821 tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca gcaccagcgc
    71881 acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgt
    71941 tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga acagcagcac
    72001 cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca tcgcctgcat
    72061 ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg
    72121 cagcccaagt aacatgcaga acagccccag caggccgccg caaatcacca tctgcaatgc
    72181 gccgccgccg aacactgccg cacgcagctt ccatagcctt tgtggatcga gttcgaggcc
    72241 gataataaac agcatcagca ccacgccaat ctcggcaaag tgcaggatag attcggcatc
    72301 ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcaatcaggt agccaagtac
    72361 cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gccgccgatc cgaggtagat
    72421 cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt
    72481 gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca
    72541 tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg
    72601 acagcacatc aaagcccgga tgtgcaccaa tttcaaaatg gctttccccg ccgccggtcg
    72661 tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc
    72721 cgtgcgagaa aactttatcg atccaaagtt tgaggagcgg tggaatgctg taccactgca
    72781 tcgggtgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat
    72841 cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc
    72901 ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa
    72961 ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca
    73021 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaat
    73081 ttaccccatt ctctgcccgc ggatcgtcat attgctggtt tgcgccagtg acatccctcg
    73141 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    73201 tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc aggtcgaaac
    73261 aatggtgcca atacggctta aggacataat cacgcccacg gcagaggcgc ggatatctgt
    73321 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    73381 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac
    73441 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    73501 gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt
    73561 ccccagtgcc gcgtttttgc caacccccaa tcccaacaaa ccaacgattt gcgggccaaa
    73621 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    73681 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    73741 atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
    73801 ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa
    73861 caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
    73921 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg cgataaagct
    73981 gatggaaaac gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg
    74041 ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacagggga
    74101 tgagacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    74161 tttgcgccgt ccgactttat cggaaatata gccaaacagc gatgtgccaa cgaacagccc
    74221 ggcgagcgtt cccgcgccca acaagccaat ccagtcagcg tccagtttta gcgccggagt
    74281 cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc
    74341 gcttccccac agcaaaatgc ggcggtgaat tgaggagaat ttgagatcgt caaagtttct
    74401 ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacga cgttacgtat
    74461 caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact
    74521 gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt
    74581 aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca
    74641 ccagcagctc cagggcctgt ttatcggcat cggccttcac aacaatgtgc ggatgctctt
    74701 catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa
    74761 aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg
    74821 gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg
    74881 cgccacgccc accgccagtt ccgggtagcc gctaaacatg cgtgggttat caaggaaggc
    74941 cggtagtttc tggtacatac gcatatcgcg cagcgggcca ctctcaagat gctgacgata
    75001 ttccgccagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc
    75061 tgcggcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat
    75121 acacattccg gcggcatcac cggcaatcaa tacgccgtca ccaaccaact ccggcagcat
    75181 gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc cacccgcgat
    75241 cagcggtgca acggccggat gttgtttgaa atcttccagc atttgcggca ccgatttttt
    75301 tgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtat tttcattggt
    75361 ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc
    75421 gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta actcgatcag
    75481 ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc ccagcttttc
    75541 ggcaaggatg gagttcactc catcagcaag gatcaccgtt ttcgcttcaa tcacatcgcc
    75601 atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc
    75661 ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc agccaggcat caaatttact
    75721 gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccattgc agtagtccat
    75781 agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac
    75841 gggggcggag tcggcgaaac caggaataat gtgttccagg ctgtgggcat agagacgccc
    75901 gccggtgacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc
    75961 ttcgcgggcg agcaccagtg cggcaaccga accggcaagc ccagcaccga cgatgatggc
    76021 gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagggccaaa atgcagccct
    76081 gccagagtgg atcaacgcgc taaagctgcg gtcagcgccg gaaggatctt cacggcgtca
    76141 ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg
    76201 aaaatggttt gcgatgcatt agcaccaacc atgtgctgga tctgcccgga gatccccacc
    76261 gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc
    76321 atccattttt cgttttccgc caccggacga gaacaggcca actccgcacc tatcgccttg
    76381 caaagctgtt ctgccagcgc aatgttctct ttgctgccaa taccgcgacc gacgctgacc
    76441 accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg
    76501 gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg
    76561 tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca
    76621 atgcgttctt cgccaatcgc cagaccaccg taaaccatgt gtttcactgt cgctttaccg
    76681 tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt
    76741 tttgccgcca gtaatttgcc gcgacgggtg tttggcagca gcaccaggcc gtctgcgccg
    76801 tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt cgatcatccg atcgtccggt
    76861 ttgccgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca
    76921 tcattgagga caaaggtgtt gatttgatta gctaaagcct gcgcaccgtt catcagttcc
    76981 ggcagacgag aaggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc
    77041 cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt
    77101 cgccttcaat cacgatgcgc tgacgttcgc gctgtttcgg cgcggcaacc tgttgttctg
    77161 accaggctgc ctctgcgtta aaaccaatat ccgccgccga ccatacctgg acgggctttt
    77221 tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggaattgata tcagtggaaa
    77281 cagcaacaac cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc agttcgcgct
    77341 caacggtgag ggtatctgca gccagggaga taattttgct gacgccgtta actgccggaa
    77401 tattgaggat ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg gaagaaccat
    77461 cgccacagag gatcaaatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg
    77521 tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccgggc
    77581 cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta
    77641 aggccgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt
    77701 tcagatcgta ttggcttatt
//
LOCUS       DAFAVF010000005        69813 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFAVF010000005 DAFAVF010000000
VERSION     DAFAVF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.005"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            33..551
                     /locus_tag="LGK42_000304"
     CDS             33..551
                     /locus_tag="LGK42_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBK2656593.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            567..1106
                     /locus_tag="LGK42_000305"
     CDS             567..1106
                     /locus_tag="LGK42_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBK2656594.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(1199..2776)
                     /gene="guaA"
                     /locus_tag="LGK42_000306"
     CDS             complement(1199..2776)
                     /gene="guaA"
                     /locus_tag="LGK42_000306"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK2656595.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2845..4311)
                     /gene="guaB"
                     /locus_tag="LGK42_000307"
     CDS             complement(2845..4311)
                     /gene="guaB"
                     /locus_tag="LGK42_000307"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK2656596.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4473..5843
                     /gene="xseA"
                     /locus_tag="LGK42_000308"
     CDS             4473..5843
                     /gene="xseA"
                     /locus_tag="LGK42_000308"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK2656597.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5840..6055)
                     /locus_tag="LGK42_000309"
     CDS             complement(5840..6055)
                     /locus_tag="LGK42_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK2656598.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6124..7596)
                     /gene="der"
                     /locus_tag="LGK42_000310"
     CDS             complement(6124..7596)
                     /gene="der"
                     /locus_tag="LGK42_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK2656599.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7714..8892)
                     /gene="bamB"
                     /locus_tag="LGK42_000311"
     CDS             complement(7714..8892)
                     /gene="bamB"
                     /locus_tag="LGK42_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBK2656600.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8903..9523)
                     /gene="yfgM"
                     /locus_tag="LGK42_000312"
     CDS             complement(8903..9523)
                     /gene="yfgM"
                     /locus_tag="LGK42_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBK2656601.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9541..10815)
                     /gene="hisS"
                     /locus_tag="LGK42_000313"
     CDS             complement(9541..10815)
                     /gene="hisS"
                     /locus_tag="LGK42_000313"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK2656602.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQYKDKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10926..12044)
                     /gene="ispG"
                     /locus_tag="LGK42_000314"
                     /gene_synonym="gcpE"
     CDS             complement(10926..12044)
                     /gene="ispG"
                     /locus_tag="LGK42_000314"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBK2656603.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12071..13084)
                     /gene="rodZ"
                     /locus_tag="LGK42_000315"
     CDS             complement(12071..13084)
                     /gene="rodZ"
                     /locus_tag="LGK42_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBK2656604.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13369..14523)
                     /gene="trmG/rlmN"
                     /locus_tag="LGK42_000316"
     CDS             complement(13369..14523)
                     /gene="trmG/rlmN"
                     /locus_tag="LGK42_000316"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBK2656605.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14673..15104)
                     /gene="ndk"
                     /locus_tag="LGK42_000317"
     CDS             complement(14673..15104)
                     /gene="ndk"
                     /locus_tag="LGK42_000317"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK2656606.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15253..17565)
                     /gene="pbpC"
                     /locus_tag="LGK42_000318"
     CDS             complement(15253..17565)
                     /gene="pbpC"
                     /locus_tag="LGK42_000318"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBK2656607.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17566..22527)
                     /gene="yfhM"
                     /locus_tag="LGK42_000319"
     CDS             complement(17566..22527)
                     /gene="yfhM"
                     /locus_tag="LGK42_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBK2656608.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22734..23579
                     /gene="sseA"
                     /locus_tag="LGK42_000320"
     CDS             22734..23579
                     /gene="sseA"
                     /locus_tag="LGK42_000320"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBK2656609.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24072..24848)
                     /gene="sseB"
                     /locus_tag="LGK42_000321"
     CDS             complement(24072..24848)
                     /gene="sseB"
                     /locus_tag="LGK42_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBK2656610.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24990..26273)
                     /gene="pepB"
                     /locus_tag="LGK42_000322"
     CDS             complement(24990..26273)
                     /gene="pepB"
                     /locus_tag="LGK42_000322"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBK2656611.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26451..26651)
                     /gene="iscX"
                     /locus_tag="LGK42_000323"
     CDS             complement(26451..26651)
                     /gene="iscX"
                     /locus_tag="LGK42_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBK2656612.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26663..26998)
                     /gene="fdx"
                     /locus_tag="LGK42_000324"
     CDS             complement(26663..26998)
                     /gene="fdx"
                     /locus_tag="LGK42_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBK2656613.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27000..28850)
                     /gene="hscA"
                     /locus_tag="LGK42_000325"
     CDS             complement(27000..28850)
                     /gene="hscA"
                     /locus_tag="LGK42_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBK2656614.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28867..29382)
                     /gene="hscB"
                     /locus_tag="LGK42_000326"
     CDS             complement(28867..29382)
                     /gene="hscB"
                     /locus_tag="LGK42_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBK2656615.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29478..29801)
                     /gene="iscA"
                     /locus_tag="LGK42_000327"
     CDS             complement(29478..29801)
                     /gene="iscA"
                     /locus_tag="LGK42_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBK2656616.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29818..30204)
                     /gene="iscU"
                     /locus_tag="LGK42_000328"
     CDS             complement(29818..30204)
                     /gene="iscU"
                     /locus_tag="LGK42_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBK2656617.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30232..31446)
                     /gene="iscS"
                     /locus_tag="LGK42_000329"
     CDS             complement(30232..31446)
                     /gene="iscS"
                     /locus_tag="LGK42_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK2656618.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31558..32046)
                     /gene="iscR"
                     /locus_tag="LGK42_000330"
     CDS             complement(31558..32046)
                     /gene="iscR"
                     /locus_tag="LGK42_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBK2656619.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32347..33084)
                     /gene="trmJ"
                     /locus_tag="LGK42_000331"
     CDS             complement(32347..33084)
                     /gene="trmJ"
                     /locus_tag="LGK42_000331"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBK2656620.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33203..34006
                     /gene="suhB"
                     /locus_tag="LGK42_000332"
     CDS             33203..34006
                     /gene="suhB"
                     /locus_tag="LGK42_000332"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBK2656621.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34151..35005
                     /locus_tag="LGK42_000333"
     CDS             34151..35005
                     /locus_tag="LGK42_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2656622.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35196..36476
                     /gene="csiE"
                     /locus_tag="LGK42_000334"
     CDS             35196..36476
                     /gene="csiE"
                     /locus_tag="LGK42_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBK2656623.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36468..37607)
                     /locus_tag="LGK42_000335"
     CDS             complement(36468..37607)
                     /locus_tag="LGK42_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBK2656624.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37767..38657)
                     /gene="hcaR"
                     /locus_tag="LGK42_000336"
     CDS             complement(37767..38657)
                     /gene="hcaR"
                     /locus_tag="LGK42_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBK2656625.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            38793..40154
                     /locus_tag="LGK42_000337"
     CDS             38793..40154
                     /locus_tag="LGK42_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBK2656626.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40151..40669
                     /locus_tag="LGK42_000338"
     CDS             40151..40669
                     /locus_tag="LGK42_000338"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBK2656627.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40669..40989
                     /locus_tag="LGK42_000339"
     CDS             40669..40989
                     /locus_tag="LGK42_000339"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBK2656628.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40986..41798
                     /gene="hcaB"
                     /locus_tag="LGK42_000340"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40986..41798
                     /gene="hcaB"
                     /locus_tag="LGK42_000340"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBK2656629.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41808..43010
                     /locus_tag="LGK42_000341"
     CDS             41808..43010
                     /locus_tag="LGK42_000341"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBK2656630.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43107..43529
                     /locus_tag="LGK42_000342"
     CDS             43107..43529
                     /locus_tag="LGK42_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBK2656631.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNLINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43577..44449)
                     /locus_tag="LGK42_000343"
     CDS             complement(43577..44449)
                     /locus_tag="LGK42_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBK2656632.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLMVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44461..45555)
                     /locus_tag="LGK42_000344"
     CDS             complement(44461..45555)
                     /locus_tag="LGK42_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBK2656633.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45588..46586)
                     /locus_tag="LGK42_000345"
     CDS             complement(45588..46586)
                     /locus_tag="LGK42_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2656634.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46611..48122)
                     /locus_tag="LGK42_000346"
     CDS             complement(46611..48122)
                     /locus_tag="LGK42_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2656635.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48145..49128)
                     /locus_tag="LGK42_000347"
     CDS             complement(48145..49128)
                     /locus_tag="LGK42_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK2656636.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49225..52506)
                     /locus_tag="LGK42_000348"
     CDS             complement(49225..52506)
                     /locus_tag="LGK42_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBK2656637.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52624..53817
                     /locus_tag="LGK42_000349"
     CDS             52624..53817
                     /locus_tag="LGK42_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK2656638.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53881..55134)
                     /gene="glyA"
                     /locus_tag="LGK42_000350"
     CDS             complement(53881..55134)
                     /gene="glyA"
                     /locus_tag="LGK42_000350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK2656639.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55462..56652
                     /gene="hmpA"
                     /locus_tag="LGK42_000351"
     CDS             55462..56652
                     /gene="hmpA"
                     /locus_tag="LGK42_000351"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBK2656640.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56697..57035)
                     /gene="glnB"
                     /locus_tag="LGK42_000352"
     CDS             complement(56697..57035)
                     /gene="glnB"
                     /locus_tag="LGK42_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBK2656641.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57096..58430)
                     /gene="glrR"
                     /locus_tag="LGK42_000353"
     CDS             complement(57096..58430)
                     /gene="glrR"
                     /locus_tag="LGK42_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBK2656642.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58420..59133)
                     /gene="qseG"
                     /locus_tag="LGK42_000354"
     CDS             complement(58420..59133)
                     /gene="qseG"
                     /locus_tag="LGK42_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBK2656643.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59298..60725)
                     /gene="qseE"
                     /locus_tag="LGK42_000355"
     CDS             complement(59298..60725)
                     /gene="qseE"
                     /locus_tag="LGK42_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBK2656644.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61301..65188)
                     /gene="purL"
                     /locus_tag="LGK42_000356"
                     /gene_synonym="purI"
     CDS             complement(61301..65188)
                     /gene="purL"
                     /locus_tag="LGK42_000356"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBK2656645.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65446..67002
                     /gene="mltF"
                     /locus_tag="LGK42_000357"
     CDS             65446..67002
                     /gene="mltF"
                     /locus_tag="LGK42_000357"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBK2656646.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNILSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66999..67535)
                     /gene="tadA"
                     /locus_tag="LGK42_000358"
     CDS             complement(66999..67535)
                     /gene="tadA"
                     /locus_tag="LGK42_000358"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK2656647.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="LGK42_000359"
     CDS             complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="LGK42_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBK2656648.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68404..69252
                     /locus_tag="LGK42_000360"
     CDS             68404..69252
                     /locus_tag="LGK42_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK2656649.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69308..69568
                     /gene="yfhL"
                     /locus_tag="LGK42_000361"
     CDS             69308..69568
                     /gene="yfhL"
                     /locus_tag="LGK42_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBK2656650.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16574 a        19239 c        17597 g        16403 t
ORIGIN      
        1 cttatttttc gtaatgtgta taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc
       61 ctgtggcaat gttagcgtca ttcactctgg caggatgcca gtcaaatgct gacgatcatg
      121 ccgccgatgt ttatcaaacc gatcaactga ataccaaaca agaaactaaa accgttaata
      181 ttatttccat tcttcccgca aaagttgccg tagacaactc ccaaaataaa cggaacgcac
      241 aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt tatcggccac aacgtcggtt
      301 ctggcagcaa ttccggaacg acggcagggg cagttggcgg cggagctgta ggcgcggcag
      361 tgggttctat ggtgaatgat aaaaccttag tggaaggtgt ttctttaaca tataaggaag
      421 gcaccaaagt gtatacctct acccaggtgg gtaaagagtg ccagtttacg acaggtttag
      481 ccgttgttat taccacaacg tataacgaaa cgcgtattca gccaaatact aaatgtcctg
      541 aaaagagcta ataatcagga ggagtcatga agaaagtttt tctttgcgcc atcttagcct
      601 ccttaagcta tccggctatc gcctcatcat tgcaggatca actctctgct gtcgcagaag
      661 cggaacagca aggtaaaaat gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca
      721 acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc ccaggccgcc gctaacaaaa
      781 gagcggcaac ggcagcggca aataagaaag ctcgtcagga taaactggac gccgaagcca
      841 ctgcggacaa aaaacgcgat caaagttatg aagatgagct acgcagctta gagattcaga
      901 aacaaaaact ggcgctggcg aaagaagaag cccgcgttaa gcgcgaaaac gaatttatcg
      961 atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcca
     1021 acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag cgtgggcaaa gcagaagaga
     1081 acaaatcgga aagctggttt aattaatcga tgttagtaac ttcaagccta tgattcttga
     1141 agataaaaaa ccctctgtag taacagaggg ttttgttcat tcatagtgca gggtcaaatc
     1201 attcccactc aatggtagct ggcggcttgc cgctgatgtc atacaccacg cgagaaatac
     1261 cgttcacttc attgataatg cggttggaaa cacgaccgag gaaatcgtac ggcagatgcg
     1321 cccagtgtgc ggtcataaag tcgatggttt cgacagcacg cagagagaca acccagtcat
     1381 acttacgacc atcgcccatt acgccaacgg aacgtaccgg caggaacaca gtgaacgcct
     1441 ggctgacttt gtcgtacagg tccgctttac gcagttcttc aatgaagatg gcgtcagcac
     1501 ggcgcagcag gtcacagtac tctttcttca cttcacccag cacacgaacg ccaaggcctg
     1561 gtcccgggaa cgggtgacgg tacagcatgt cgtacggcag gcccagctcc agaccaatct
     1621 tacgcacttc gtctttgaac agctctttca gcggttcaac caggcccatc ttcatctctt
     1681 tcggcaggcc gcccacgttg tggtgagatt tgatgacgtg tgctttaccg gttgcagaag
     1741 ccgcagattc gataacgtca gggtagatgg tgccctgcgc cagccacttc acgtcttcca
     1801 gtttcagcgc ttcttcatcg aacacttcaa cgaaaacgcg cccgatgatt ttacgttttg
     1861 cttccggatc gttttcgcca gccagcgctg acaggaagcg atcttctgcc ggtacgtgaa
     1921 caatgttcag accaaagtga tcgccaaaca tatccagaac ctgctctgct tcgttgagac
     1981 gcagcaggcc gttgtcgacg aatacgcaag tcaggttttt accgatagcg cggtgcagca
     2041 gcattgcggt tacggaggaa tccacaccac cagagaggcc gaggatgact ttatcgtcgc
     2101 ctacctgctc gcggatgcga gctacagcat cgtcgataat tttcgctggc gtccacaggg
     2161 cttcacactg gcagatatca cgcacaaaac gctccagcat gcgcataccc tggcgggtgt
     2221 gagtcacttc cgggtggaac tgtacgccat agaagcgttt ttcttcgtta gccataatgg
     2281 caaacgggca gctttcggtg ctggctacgg tgacgaagtc ggacgggata gcggtaactt
     2341 tatcgccgtg gctcatccag acatcgagca gcggtttacc gtctgcggtc agcgcatctt
     2401 cgataccgcg aaccagtgcg ctgtcgttta cgacttcaac ctgcgcgtag ccaaattcac
     2461 gttcgttaga ggcttcaacg tgaccgccca actgcattgc catggtctgc atgccatagc
     2521 aaacgccgaa taccggtacg cctgcttcaa agacatactg cggcgcacgc gggctgtttt
     2581 cttcggtggt gctttccggg ccgccggaaa gaataatgcc gcttggattg aagtcacgaa
     2641 tttgtgcttc tgtcacatcc cacgcccaca gttcgcagta aacacccagc tcacgcacgc
     2701 ggcgcgcaac cagttgagtg tactgagaac cgaagtccag aatgaggatg cgatgcttat
     2761 gaatgttttc cgtcattgac gcttattccg aggcaagtga aacagataat ataaatcgcc
     2821 cgacatgaag tcgggcgaag agaatcagga gcccagacgg tagtttgggg actctttagt
     2881 aatggtcacg tcgtgaacgt ggctttcctg aatgcccgca ccgctgatac gtacaaactc
     2941 cgctttagta cgcagttcgt cgatagtacc acagccggtc agacccatac aggagcgcag
     3001 gccgcccatc tgctggtgaa tgatctcttt caggcgacct ttataagcta cgcgaccttc
     3061 gataccttcc ggcaccagtt tgtcggcagc gttatcgctc tggaaataac ggtcagagga
     3121 acctttggac atcgcgccca gggaacccat accacggtaa gatttgtaag aacggccctg
     3181 gtagagttcg atttcacccg gagattcttc agtacccgcc agcatggaac ctaccatcac
     3241 cgcgcttgcg ccagcggcga tagctttggc gatgtcgccg gagaagcgaa taccaccatc
     3301 tgcgataacc ggaataccgg tgccttccag ggcttcaact gcgtcagcaa cggcagtgat
     3361 ctgcggaaca cctacgccag taacgatacg agtcgtacag atagaaccag ggccgatacc
     3421 gactttaacc gcgctgcaac cagcttctgc caaagcgcgt gcacctgcag ctgttgccac
     3481 gttgccgccg atgatttgca gatccggata tttagcacgg gtttcacgga tacgctgcaa
     3541 aacgccttct gagtgaccgt gggaggagtc aatcagcaga acgtcaacgc cagcggcaac
     3601 cagcgcgtca acacgctctt cgttacccgc acctgcgcca actgcagcac caacacgcag
     3661 acggccttgc tcgtctttac aggcgttcgg tttacgttcc gctttctgga agtctttcac
     3721 ggtgatcatg ccgatcaggt ggaattcgtc atcaaccacc agcgcttttt caacgcgttt
     3781 ttcgtgcatt tttgccagca ccacttcacg ggcttcacct tcacgcacgg tgaccagacg
     3841 ctctttcggc gtcatgtaaa cgctaaccgg ctggttcagg tcggtaacaa aacgcacgtc
     3901 acgaccggtg ataataccca ccagttcgtt ttcttcggta acgaccggat agcccgcaaa
     3961 accgttacgc tcagtcagtt ctttcacttc gcgcagcgtc gtggttggca gaacagtctg
     4021 cggatcagtc accacaccag attcgtgttt tttcacacgg cgaacttctt ctgcctggcg
     4081 ttcaatggac atgtttttgt ggataaagcc gataccgcct tcctgagcca gagcaatagc
     4141 caggcgcgct tccgttacgg tatccattgc tgcggaaagc atagggatat tcagacgaat
     4201 agttttcgtc agctgggtgc tgaggtcagc agtattcggc agaacggtag aatgagcagg
     4261 aacgaggaga acgtcgtcaa acgtcagagc ttctttagcg atacgtagca tgggcaatat
     4321 ctcgaccaga gtggttaata aatattgccg cggcattata cagagcgtaa ccgattgcat
     4381 ctaccccttt ttgcaaaaaa tgcttgctat ccccgaaggg cgggttacta tcgactgaat
     4441 aacctgctga tttagaattt gatctcgctc acatgttacc ttctcaatcc cctgcaattt
     4501 ttaccgttag tcgcctgaat caaacggttc gtctgctgct tgagcatgag atgggacagg
     4561 tttggatcag cggcgaaatc tctaatttca cacaaccggc ttccggtcac tggtacttta
     4621 cactcaaaga cgacaccgcc caggtacgct gcgcgatgtt ccgcaacagc aaccgccggg
     4681 tgaccttccg tccacagcat ggacaacaag ttttagttcg cgccaatatt acgctctacg
     4741 agccgcgcgg tgactaccag ataatcgttg agagtatgca gccggccggt gaagggctgc
     4801 tgcaacagaa gtacgaacag ctcaaagcga agttgcaggc tgaaggtttg ttcgatcagc
     4861 aatacaaaaa accacttccc tcccctgcgc attgcgttgg tgtgatcacc tcaaaaaccg
     4921 gtgctgcgct acatgatatt ttgcatgtgt taaaacgtcg cgatccttct ctaccggtga
     4981 tcatctaccc caccgccgtt cagggcgatg acgcaccggg gcaaattgtt cgcgccattg
     5041 aactggcgaa tcagcgcaat gagtgcgacg tgttgatcgt tgggcgcggc ggcggttcgc
     5101 tggaagattt atggagtttt aacgacgaac gcgtagcgcg ggcgattttt gccagccgca
     5161 ttccggtcgt cagcgccgtc gggcatgaga cggatgtgac cattgccgat tttgttgccg
     5221 atctgcgtgc gccaacgccg tctgccgccg ctgaagtagt gagccgtaat cagcaagagt
     5281 tactgcgcca ggtgcaatcg acccgtcaac ggctggagat ggcgatggat tattatctcg
     5341 ccaaccgcac gcgtcgcttt acgcagatcc atcaccgatt acagcaacag catccgcagc
     5401 tccggctggc acgccagcaa accatgcttg agcgcctgca aaagcggatg agctttgcgc
     5461 tggaaaatca acttaagcgt accgggcaac agcagcagcg gttaacacag cggctgaatc
     5521 agcaaaatcc acagccgaag attcatcgcg cgcaaacgcg cattcagcaa ctggaatatc
     5581 gtttagcaga aaccctgcgc gtacagctta gcgccacgcg tgaacgtttc ggtaatgcag
     5641 taacgcacct cgaagccgta agcccactgt caacgctggc gcgtggatac agcgttacta
     5701 ctgctactga cggcaatgta ctgaaaaaag tgaagcaagt taaagcgggt gaaatgctaa
     5761 ccacacgtct ggaagacggc tggatagaaa gtgaagttaa aaacatccag ccagtaaaaa
     5821 aatcgcgtaa aaaggtgcat taagccagca cgaattcaac gcgttttttc gatatcaagc
     5881 catgcccgtg ctgacagaag taatctactg caccgcaggc ttttaacacc tgcaacggtt
     5941 gatggcagtc tggacaaaga gctttcattt caataatttc cccgcaggat gggcaacgcg
     6001 catggccgtt atcctgatca agcacatgct gacattgtgg acaatgcagt tccatatgaa
     6061 ttcctccggt agccatccct ctacattcat agagggaatg gcagataaaa tacttacgga
     6121 taattattta tttttcttga tgtgcttcat cagacgctta cgtttacgca tctgggttgg
     6181 cgtcagggtg ttacgcttgt tcgcatacgg gttttcccct tctttgaact gaatacgaat
     6241 cggcgatccc attacgtcca gcgatttgcg gaagtagttc atcaagtagc gcttgtagga
     6301 atcaggcagg tctttcacct gattaccgtg aatcaccaca atcggcgggt tataaccacc
     6361 agcgtgggca tatttcagct tcacacgacg accgcgtacc agcggcggtt ggtgatcttc
     6421 aacagccatc gtcatgatgc gcgtcagcat agaggtcccc acacgacggg tggagctgtc
     6481 atacgcttca cgtactgatt caaacaagtt accaacacca ctgccgtgca aggcagagat
     6541 aaagtgcaca cgagcaaaat cgataaagcc cagacggaag tccagcgttt ctttcacctg
     6601 ctctttcact tcctggctca ggccatccca cttattcacc acaatgacaa gtgagcgccc
     6661 actattgaga ataaagccca gcagcgagag atcctgatcg gaaatacctt cgcgcgcatc
     6721 aatcactaac atcaccacgt tggcatcttc aatggcctgc aacgttttga ttacggagaa
     6781 tttctctaca gcatcggtga ttttgccgcg tttacgtacg ccagcggtat caatgagcac
     6841 atactcacgt ccatcgcgtt ccattggaat gtagatactg tcacgcgtcg tgccaggcat
     6901 gtcataaaca acaacgcgct cttcaccaag aatacggtta gtgagtgtag acttacctac
     6961 gttcggacga cccacaatcg ccagtttgat cggcagactt tgcgggtcga agtcgtcttc
     7021 ctcttcttct tcgccgttct cttccgcttc aaattgcgcc cagtattcag cgtcttcgtc
     7081 gacttcctct tgcggtgcga gatcttccat ccacggcagc agcacatgct ccagcagact
     7141 taatacgcca cgaccgtgag acgcggcgat cgggtagatt tcacctaaac caagcgagta
     7201 gaaatcaacc actgcctgat cgggatccag accgtcagtt ttgtttgcca ccaggaaggt
     7261 cggtttttca cgggagcgca gatgtttggc aatcgcttca tctgccggca tcaggcccgc
     7321 gcgcgcatcc accataaaca gtacgacgtc cgcttcttca atcgccagca gcgactgttc
     7381 cgccatgcgg gtttctacac cgtcttctgt gccatcaatc ccgccggtat caatacagat
     7441 aaactcacgg ccttcaattt ccgcacgacc gtacttacgg tcacgagtca gacccgggaa
     7501 atccgcaacc agcgcatctc gggtgcgagt tagacggtta aataacgtgg attttcctac
     7561 gttagggcgc ccgacaagcg cgaccacagg taccatgttt aaagcctcat ttttataaat
     7621 catcagacaa cgcacgctat attcgcgtcg ttgttaaaaa caggaaaacg gcccctgtcc
     7681 aggagccgtt ttcaaagtga acgacagaga cgattaacgt gtaatagagt acacggttcc
     7741 gtcttttgcc tggatcagca gtttgccgtc agcggcaacc ggttcagtct ggaaaccgga
     7801 actatcaact ttttgctggg caacgaaacg accatcttcg acgttaatcc agtgcagata
     7861 accttcactg tcaccgacca ccaggttgcc attatacagc accggagaag tcagcaggcg
     7921 atgcagcaga tcgctttgtg cccacagcgt aacgccgcca tcaatggtca acgccatcac
     7981 ccggtcattt tgatcgacca gatagatgcg attgccgtcg acgatgaaat cattcaccga
     8041 acccagttcg cgtttccaca taatctgacc actgcgcaga tcaagcgccg tcaggttacc
     8101 attataggcc agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt caacatcgct
     8161 cagacggtca atttcggtag aaccggtcgc ctgggaaata cgctgctgcc aaatcatctg
     8221 gccctgttcc atcagcactg cgctgacgcg accattatcg ccccccacga cggccgcacc
     8281 aaaagccgtt gccggcgcag actcgccacg caaagagagc gaaggcatat cgaggttaac
     8341 tgtccatttg acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt tactggtgtg
     8401 gattaacacc agaccgtcgc tgaccaccgg gcgcgaaagt gcttcacccg cgactttagt
     8461 ttgccatgcc acagtaccat cgctggtatt cagcgcgtaa acctgcgcct tttcgctgcc
     8521 aatgtagaca tgcccaccag acacggtcac accgccagaa agtaatgcag gctctttaga
     8581 gaaccagcca tctttctcgg ccaggctgac agaccagatt tctttgccat catccgcatt
     8641 cagcgctttt accaaaccag cgcggtccgc tgcatagaca acgttgtccg ccagtgccgg
     8701 atgaagattg gaatagaagt tgccaatgcc gctaccaacg gaagtactcc acgccgtggt
     8761 cggcgtaaac tggttttcaa cggttggcaa tggggacatc tttaccacat cttcttcgct
     8821 gttaaacagc gaacagccgc ttaaaagggt aacggaaagc agtcctggca gcagtaattt
     8881 acgcaattgc atcgggtccc tctcagatgg acaaattatt aattttcatc tgcatcattt
     8941 cgctcagtgc cggagtaaca tcgcttttca cgcctgcttc ccatgcacta cgcgcacctt
     9001 gcttatcacc tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg gcagcccacc
     9061 cttcaccttt gatggtatca agggttttca gcgcggcatc agcctgcttg agctgtacct
     9121 gaacgcgagc aagacgcaga tttatcacgg ctttgagatt ttcatcgctc gtgtctgcca
     9181 gcccctgttg taactgggcg gcagctttct ctagttcatt tttgtcaaca aattgctgcg
     9241 caagttccaa agaagccagc gcaccataag tatttttatt ttcagcagca aatttttccg
     9301 ccgccgggat gctatccggt ttgccttcgc tcactgcggt aaccgcattt tgataggcaa
     9361 gagaagcgga gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag ccaatcagtg
     9421 cgccaacgcc caaaatcacc ccaacagcca gtgctttgcc attttcagca aaaaagcgtt
     9481 taaccgcttc tacctggtcg ttttcgttct cgtaaatttc cacgctgtcc ttctccttcc
     9541 ttaacccagt aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg ccgtttgctc
     9601 accagagcgc aaatccttca ctactgctgt gccgttagcc acttcagact cacccagcac
     9661 cacagcaacg cgggcacccc atttatcagc acgggcaaac tgtttcttaa agttgccgcc
     9721 gccgtggttg gtcatcaatt tcacgcccgg taattcatca cgcagacgct cagctaatgc
     9781 catagccgca gattgtgtat cagcacctga agccaccagg tatatatcga caacaggatc
     9841 ggctttaaat tccggattaa cggcctgtac taacaataca agacgttcga ggcccatcgc
     9901 aaaaccgaca gccggtgttg cacgaccgcc cagttgttcc acaagaccgt cataacgacc
     9961 gcctgcacac acggtgccct gggagccgag actgttagtc acccactcga aaacggtacg
    10021 gttatagtaa tccagaccac gcaccagacg ctggtttacg gtgtaagcga tccccgcgct
    10081 ctcaagcagt ttgcacagac cggcaaaatg ctcacgagat tcctcgtcca gataatcacc
    10141 taatgccgga gcgtcgttga gaagcgcctg cacttccgga ttttttgaat ccagcacgcg
    10201 cagcgggtta gtgtacatgc ggcgtttgca gtcttcgtcc agcttatctt tatactgctc
    10261 aaggaatgcc accagcgcat cgcggtaatt ggcgcgtgct tccagcgaac cgatagagtt
    10321 cagctcaaga gttacgtgct cggaaatacc cagcgcacgc caccagcggg cggtgagcat
    10381 aatcagttca gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc ccaactgatg
    10441 gaactgacga taacgccctt tctgcggacg ctcgtgacgg aacatcggcc cgatatacca
    10501 cagacgctgt tcctgattgt acagaagacc atgctcgatg ccggcgcgta cacagcccgc
    10561 cgtcccttca gggcgcagag tcaggctgtc gccattgcga tcctcaaagg tgtacatctc
    10621 tttttcaacc acgtcggtga cttcaccaat cgcacgtttg aatagcgggg tctgctctac
    10681 aatcggcaag cggatttcac tgtaaccgta gctgccgagc acgtttttca gtgtgccttc
    10741 aatgcgctgc cagatggccg tttcgccagg caggtaatcg ttcatgccgc gaatggcttg
    10801 aatgtttttt gccacgttta ttctctttct gaatataaaa atgaaccctc aacgcttccc
    10861 tcaatgtttc gggagctatg cgggttcaat catacacggg aagcgaggcg cttcccatca
    10921 cgttattatt tttcaacctg ctgaacgtca attcgacgcg cttcgtccag ctgactggct
    10981 ttcgcacgaa tgcgtgcttc cagctggtcg atcatatcgt tgttgtccag acggtctttg
    11041 cgcacgccat cttcatagag gccgcttttc ttgttgccgc cggtgacgcc gagtgtagaa
    11101 accagcgcct cacctgggcc attcaccacg cagccgataa tcgaaacgtc catcggagtg
    11161 atgatatctt ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc aaattcctga
    11221 cgcgaacagg tcgggcaggc gatgaagttg atcccgcgcg aacggatacg cagcgatttc
    11281 aaaatatcga aaccgacttt gatctcttcg accggatcgg ccgccagtga tacgcgcagc
    11341 gtgtcgccga tgccttcaga cagcagcaga cctaaaccaa tggcggattt tactgccccg
    11401 ctgcgtgcac caccggcttc ggtgatcccc agatgcagcg gctgatcgat ctgttttgcc
    11461 agcaaacgat aagactcaac agcgaggaag acgtcagacg ctttcacgct gactttgaac
    11521 tgatcgaagt tcaggcgatc gagatgatca acatggcgca tggcggattc cagcaacgcc
    11581 tgcggcgtcg gttcgccata cttttcttgc agatcttttt ccagcgatcc ggcgttaacg
    11641 ccaatacgga tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg aatacgctct
    11701 tcattaccga tattgccagg gttaatacgc agacaatcga cgccgtattc cgctactttc
    11761 agtgcaatgc gatagtcgaa gtggatgtca gccaccagcg gcacgttaac ctgctgtttg
    11821 atgagtttga acgcttctgc cgcgtccatc gtcggtacgg agacacggac gatatcagcg
    11881 ccaacgcgtt ccagcgcctt gatttgattg accgttgctt cgacgtctgt cgtacgcgta
    11941 ttggtcatgg actgtacggc gatgggagca ccatcgccaa tcggcacatt cccaacgtaa
    12001 atacgtgttg attttctacg ttgaattgga gcctggttat gcatgaaaaa tctcccgcgt
    12061 tacccgtctg ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc gcaacctggt
    12121 tagttctgat aaaacgactc agatcgacag gtttcccttg atactggatc tgtactgcgg
    12181 ctggcgcacc aattttcagt ttgtatggtg cctggccggt taagttcaaa ttaccgtctt
    12241 tacgctgcat accgctaaac aattttttac cggtagcatc agtaacctcc agccagcaat
    12301 cggcagtaaa gttcatcacc agcgcattcg gatcagccgc cggcgtagtc acgcctgcct
    12361 gatcggttgg caacggcgca gcgccatctg gcgttgttgt tgccgttggt gccggggtcg
    12421 cggcggtatc aacatttgcc tgcgaaggcg aaacaaccgc attctgttgc ggatcaacag
    12481 ctggtgctgg cgcagttacg gcaggtgttt gcgtgttggt tgcggtagta tccacagacg
    12541 ctggcggcgt ggacgttgta gccgggtctg tagttgtcga cgtatttaac ggaacactct
    12601 gcccctgctc gctattactg ctcagttccg ccgaagattg atcggccata gtggtgatct
    12661 cttcctgctg agctttgtgg tcttgccacc accaggcacc gctcaggccg ataaccacaa
    12721 acaacaccag ccaggtgaag gtcatcagcc agccgtcacg ttttttgcgg cgtttaccga
    12781 gggaaaaact ctgcataggc gcaacttttg cagcccgtag tggagcctgc ttttccagcc
    12841 ctggcagcag ttcttcttct ggaatatgta ccagacgcgc ataagagcgg atatatccgc
    12901 gcaggaatgt tgaagcaaga tcggcgggtg ccttatcttc ttcaatgtcg cgtaccgtgg
    12961 aaaccttcag gcaaagtcgc tcggcaacgg cctgctgact aagtcctagt tgttcgcgag
    13021 cattacgcag gcgagcgccg gtagtaagtg cttcattttg gtcgtgcgtg gcttcagtat
    13081 tcattcgctg caggtacgtt taaatgagaa ttgagatgcc ggtgaaccat catgctcacc
    13141 cacaccgcga aacatccgtt aagttaaccg ttatcataca gtataagacc gtctgtgtgc
    13201 tcgtgacaaa gcccacattc actaaggcta acttactgtt gcatcgttac atactgcctt
    13261 aaagtcagca aaaacgcacc gttaatattg accagacaat tgcaacttaa tacttcattc
    13321 ataaaagatt acgccacggt acataaagta accgtggcgt aatggctatc agaccgcttt
    13381 aatgtcgatg gcttcaccct gcatccgttt acgcagggta cgtttcgtac ggtcgataac
    13441 atcgcccgcc aactgaccac aggcagcatc gatatcatca ccacgggttt tacgcacaat
    13501 agtggtgaaa ccgtagctca tcagcacttt tgagaaacgg tcgatacggc tgttcgagct
    13561 gcgtccatac ggcgcgcccg ggaaggggtt ccacgggatc aggttgatct tacacggcgt
    13621 atctttcagc agttccgcca gttggtgcgc gtgttcagtg ccgtcgttaa cgtggtcaag
    13681 catcacatac tcaatagtga cacggccctg attggcgttg gatttctcca gataacggcg
    13741 caccgcagca aggaacgttt cgatattgta ctttttgttg atcggcacaa tttcgtcacg
    13801 aatttcgtcg ttcggcgcgt gcagggaaat tgccagtgca acgtcgatca tatcgcccag
    13861 tttatccagc gccggaacta caccggaagt agaaagcgtg acgcgacgtt tagacaggcc
    13921 aaaaccgaaa tcatcaagca tgatttccat cgccggaacg acgttgttca ggttgagcag
    13981 cggctcgccc atgcccatca tcactacgtt agtgattggg cgctgaccgg tgacttttgc
    14041 tgcgccgacg attttcgccg cacgccacac ctggccaata atttccgata cccgcaggtt
    14101 gcggttaaag ccctgctggg cggtggaaca gaatttacac tccagcgcac accccacctg
    14161 cgaagagacg cagagcgtgg cacggtcgtc ttccgggata tacaccgttt cgacgcgctg
    14221 atcgccaacg gcgatcgccc atttaatggt gccgtcggat gaacgctgtt cttcaaccac
    14281 ttccggtgcg cggatttccg ccacctcttt cagtttgccg cgcaacactt tgttgatgtc
    14341 ggtcatctca tcaaagttgt cgcagcaata gtgatacatc cacttcatca cctgatcggc
    14401 gcggaagggt ttttcaccta aatctttaaa aaactcccgc atctgctgac ggttgagatc
    14461 cagcaggttg atttttccat ctttcgtggt gacgttttca ggtgtgacta attgttcaga
    14521 catatgctat tccggcctcg ttattacacg ttatggcccc tggaggattg aaaaaagaaa
    14581 cgccccggtg agcggctgct cgtccggggg cgctgcattg tacaaattct ggcgcacgga
    14641 tgccacgttt gcacgcggca tttacgaaat tattaacggg tgcgcgggca cacttcgcct
    14701 tcgccaaaga aataagcgat ttcgcgagcg gcagattcga cggaatcaga accgtgggta
    14761 ccgttttcgg tcaggctgtc agcgtaatca gcgcgcagag taccagccag tgcgtttgcc
    14821 ggattggtcg cgcccagcag atcgcggtga cgctgaacgg cgttttcacc ttccagcacg
    14881 gaaaccacga tcgggccaga ggtcatgaat tcaaccagac catcaaagaa cggttttcca
    14941 tcgtgttcag cataaaagcc acgtgcctgt tcaacggtca ggtgcagcat tttggtgccg
    15001 acaattttga accctgcagc ttcaaagcgc gcaaagatat taccaatgac gttttttgct
    15061 accgcgttcg gtttgatgat ggaaaaagta cgttcaatag ccatttttac ctctgtaaat
    15121 tgttctgttg ttgtctgtac cagcgtacga gatggcgcgg attataatga gcaacagggc
    15181 cgttgactat tgatgaaggt aacatttttt taaaataaaa tgagttttag caacaatcag
    15241 ggacaaaaca gactattgca tgacaaattt cactgtcgcg atttgcccca catcatccat
    15301 caccagcaat tgataatcac ctttatccgt caaatgcagg gtcacgttgc gcccgcgttc
    15361 agttaacggt tcgccattca gaaaccacca gcgttcacct gcccctccac ttgattgcaa
    15421 cggcaaagtc gcttccgctg cgcccggtaa acgtttaata atcgcgccat cgcgaacgcc
    15481 tgtcagctgc agagggagct gggcatcgtg accgtatgac gggcaaattg tcgaggccgg
    15541 tggtaagcgc acagcgcgac gttctgatgc gggcagccag ggttccagcg gtagcggcca
    15601 gacatttatc atttcttgtc gcgcttgcgg gcaatcagcg gcaacacgtt tgccattttc
    15661 atccagccag atggggaaac gaatgccatt aatgccttcc tgctccggca gtaatagagt
    15721 tggcggctga ctcccgtcca gcagccaggt tgccaggcgg cggcgacagt taccgtcacc
    15781 ttccggcaaa gactgtccgc ccggccagca gataacgcca cgagtgactg agttcgggcg
    15841 cgggtcttcc ggcagattcg cactgcgcga cagtaagata ttattgacct gattcaacaa
    15901 tggtacggca ctggcaaagc caaactgacc aacaacgggc gtgccgtccg gtctgccagt
    15961 ccagatccca atgacatagc gagcgttaac cccaatcgcc caggcgtcac gatagccata
    16021 gctggtgccc gttttccatg ccagtggggc gacgcgcggc aaggcactat ccggcaaggg
    16081 tttcgcttca tcagccataa tccggcgaat gatccacgcc gcccccgacg acattaaagg
    16141 ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg cctgccttgc cgtggcgagc
    16201 aaacgcggta tacgctgccg ccatatcttc cagttttgca ccagcgccgc cgagaatgag
    16261 tgaaagattc ggcgcagcac cgttgggcaa atataacggc aatccaacat tgcgtaactt
    16321 tgccgcaaac cgtttcggtc cataggcttc cagcacctgc acagcaggta agttcagcga
    16381 gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa ccgctatcaa agttacctgg
    16441 tcgataatca ccggtgcgcc gggggacgtc ttgcagcagt gatgccgggt ggatcaagcc
    16501 ttcatccagc gccagaccat aaacaaacgg tttgagcacc gatcctggcg atcggatcgc
    16561 attgaccata tcaacatgac caaagcgtga atcatcgttg agatcaaccg atcccaccca
    16621 gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc gccagtgagc tgcgcggtgg
    16681 caaccgacct ttccagtttt gcgccagttc ttccagacgt cgttgaagac cggcatccag
    16741 cgtagtaacg attttgtcgc ttttgctttt accgagcatc atgcgcgaaa acagcggtgc
    16801 cagttgcggc atttgtcggg gtgccagcca gatgggttct tcccttgact cttttacctg
    16861 ctcacgggac cacgcacctt gcacggccat ccgttcgagc actttattac gcgcggcttc
    16921 ggcacgctcc ggccaacgat ccgggcgaag acggctgggc gcttgcggca aaaccgccag
    16981 cattgccgcc tcggaatagc ttaaattcgc aggcgacttt ccgagataag cccaacttgc
    17041 cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg ttaagataca aggtcagaat
    17101 ttcacgctta gacagatgcc attccagttg caacgcgcgc cagagctggc gaattttgcc
    17161 gccaaatgtt ttggggtgag gatcaagcag acgagcaacc tgcatagtga gcgtgctgcc
    17221 accggaaata acccgtcccg aagtgagatc ttgccatgct gcgcgcgcca ccgagaatgg
    17281 attcaccccc ggatgcttcc agaaccagcg atcttcataa ttgatcagcg cttcaaggta
    17341 acgtggagaa acatcttcga ttgttaccgg ataacgccag atgccgtcag catcggcgaa
    17401 gcgccatagc ggcgtaccat cctgcgccac gaccactcgt gcgggattga cttcatgcag
    17461 cggtagaggc cagagtttat ctgctcccca cgctgcaaga aaaaggagaa atggcgcagc
    17521 tgccagcgtt atccagcagc cgcgtttggt taacaagcga ggcatttacg gtcggacaat
    17581 cagcagatct tcagccgcgc cggtcgcccg ccattgggga acatacattg attccaccat
    17641 cggttgcggt acctgatacg ttcccggcgt caccgcccgc gccagataca ccagcgttac
    17701 cggttggtat tcatcaacgg caaccgccgc cacaaagcga tcgtcacgga actcaatgtg
    17761 cttaatgctc gcctgctgca tctggttcag taagttttgc acttcgccac cactttgctc
    17821 caggctggcg ctaccgttcg ccagattctg attttccagt tccagacccg caggcagcag
    17881 atccacgact aacgcatccg gcacgctgtt actggctttt acctgcaacc agaccagcac
    17941 cagatcgccg ctacgtaacg agtccagcga tttgctctta ccatcagtac caagaatatg
    18001 acgctcgatt tgcagcacat tgttcgccgg taaaggtgcg gattgcggat aaccgctggc
    18061 atccatacgc aaccataacg gctgatcgcc actgtttctc acctgcaagg tggcaagttg
    18121 atcgctattc agattgctgt ttagcgtttt ctcgcctgtc agcggctcag ctgagaaaga
    18181 ggtttgcgcc tgccatttac ccggtaaatc ctgaatcgta cgggcagcca ggaacaacgc
    18241 gttactttcc tgcgtcgata gccagcgttc accaaatgcc tgctgcgaaa gcgtgttcag
    18301 caaagtgttc tgctcatcgg gtagcagttt attttcttcc agcaaggaga gcattaacgc
    18361 gttgtcgcgc agtggactac cgtaatcacc cagccatatc cgctcatcac tatttctcgg
    18421 cgttttcagc gccagcacaa tcgcttcttc accacgcgtt gcatcaccca tggttttcag
    18481 cgcaacgcca agttgcagca gcggtaaacc agaagcggca tctgcacgat gctcccagat
    18541 ttcacgcagc gcacccagtg gtgctttttg ctgacgggcc agcaccagcg cagcgtaaga
    18601 ctgaacggcg aatttactgg ctttgagatt atccgcgtac gggatcgaca tcatgcccgg
    18661 atcttgtaaa tagcgcagca gacgctcatt accccggtta atggcgtctg tcggcacgct
    18721 gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg taagccgtca gccagtactc
    18781 ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta tcacgttgca tttgcagcaa
    18841 acgggaaatg ccgatatcga ccgatgcacg gcgtttctca tcactgtcgc ctttgatgcc
    18901 caacgcctgc agttgggctg cgttggtata aagtgacggg aacaggccgc tggcggtttg
    18961 ctcaagacag ccgtacggat acgcttttaa ctctttgata taacgtgcaa tgttcagcgg
    19021 tggtttgccg ctcaacaaca attgcccttc cagcgtaaca ggcgagaagt tttgcaatcc
    19081 atccgccgga atcgcccatg tctcaccagg ctgtaacgcc gtaccgtaat taaccgtttg
    19141 tgccgggaac gccggacgga cgccgatttt ccactgctta tgctgatcgg caacggtttc
    19201 acccggtaac gctaacccgc taatggtggc ctgaatttct ccatcgccat aacccggcaa
    19261 tgctcgcacc gggataaaca gcgtagtacg cacacctggc gctaattcaa cggctgcggg
    19321 tgaatcgctg accagttcaa gcaaaccact ggcggtcagg gcaacgttca gtttttgcgg
    19381 tttatcggta agattagtga tatccagcgt cagacgcgag gtatcgccac tcgccataaa
    19441 gcgcggcatg ttcagttcag caatcaccgg tgcggcaact atcactttgc tttcgttgct
    19501 accgaaatca tctgccgtcc aggcttgcgc catgacgcgc aattcaccgt taaaatcgcc
    19561 aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc gttaccggca gcgcctgctg
    19621 cgcgacaata ttgacgtgat tgaccggcgg tttaccacca cgtttcagct catcaccatc
    19681 gccaccgaaa cgcagagctg ccagacgccc ctgaccttca ataacctgac cgtaaatatc
    19741 gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc tgccacggat ctggcgtgac
    19801 gtagtcagta atattcagca caccactatc aacggcagac accagcacat tcacctgttt
    19861 aggcttctcg ccatttttag tgctggcttt aattttcacg gttaatggtt gattcggccg
    19921 catttttgct ggtgtttcca gcgccagatc gaggcgacgg ttttcatcgc caagcggcag
    19981 atgcaacacc ccaactgcgc gttttggcgt cgcggagcga gatttatcgc caggacgtac
    20041 taccagcgta cttaaataca gatcatgacg attccaggtt ttatcgaccg gaatcgtcag
    20101 atccagccct tgagccggaa catcaatctc ttgccaccac aacggccctt cactggactc
    20161 gaccatcgca taacctttac ccgccgttgg cgcggcaata tgcaacttaa tggtatcgcc
    20221 agggcgataa ctggctttat ccagtttcag cgtgacacgg tcgggtcgca cggcaccgct
    20281 cccgtcgctg ttgtcctgcc agctatagcc agcccagaaa cgcacgctac tgaccgcttc
    20341 attcggcgct ttgacttcca gacgataagc gccccactct accgggaaac tgaccttacc
    20401 ggtttcatcc gcctgaagat ccagagtttg ttcattttcg atcagatctt tttgatcaaa
    20461 ctgtgactgc cagccttcat cttctgacca gttccagtag taatcgcggc gttcgcgaat
    20521 caggcgcacc tgcaagcccg acacggcttt tttcacgcct tgcgcatcgc tataaacgat
    20581 gtcaaaaccg gcgttactgc cttcatcaac aatgggctgt ttcaccgtgc tgtcagtgcg
    20641 ataatcgtaa accgatttcg aggcgaactg cggacggatc cccggcaatg catcggcagg
    20701 ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg cccgattcca gcaaactacc
    20761 ctggaaaata acctgtaatg gggaatgcgt ttccttccac tggctttctg tagaaacttc
    20821 gccgcgccct ttatcatcca gcgtcaactg aacttcatcc agcgtgcggg aaagattttc
    20881 ggcagctata tcgccgaatt caaaaccagg taaggctgac acagcttcac gcagtgggcg
    20941 caggaaaagt tgcccttgca aagtattacc attagcaggt gcaccataca ggtagtaccc
    21001 caccacggag aatttcactt catctttcgg cgttagcggg gttttctcac cggtcagatt
    21061 cagcgccatg cgctctggca taaaatcttc gacgtggaaa tcccacatcc gatactggtt
    21121 atcgcccgtg ttagcgcgaa tatgccacat accggttgcc gcattgctat cgagtggcca
    21181 ggtaaagtgg tagaggccat tctccggctg actaacgacg ctcctgagta cctgcccatc
    21241 gggtttaatc acgtctaact tgatgggttg attgggcaac gctttaccgt ctgcatcacg
    21301 cagcaaacca ttgaggatta ccgtttcacc tgggcgataa agatcgcgtg ggccaaacat
    21361 gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta aattctgcta agtccagcgc
    21421 cggaagtttt aaatcgagta gcgttgtctg accgtcttta cgcgccaaca gtaatgccgc
    21481 gtttttatca ttttccagct gcacatgccc ctgagcgtca ctggttgcct gagtcagagt
    21541 ctgccctttc tcatttaata aagagacttc aattccttgc tgggccgcgc cgttttccag
    21601 actttgggta aagatatcca gacgattgtg ataacggtga gctgaaacgc cgatatcact
    21661 taacgtaaac agcgtcgcgg gattactgta atcgtaacgt ccagcctgat tcatcacagc
    21721 cagatacacg cccgcctgct gaagcggttt gatatcgccc agcggcagca ataatttttc
    21781 acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg tagaccagat ccgccatctg
    21841 cagcagtttg tctgactgcc agttcgccag cgaattgcgg tattcccatt ggctaatgaa
    21901 tgctggcaga gattctggct taacgcggaa gaagttaaca tcgacattat taacgttgag
    21961 cgccattacc ggcagccctt caacgacttt accaggcagc agcgaaccac ggctggcaaa
    22021 accgacgctg ggttggatgt cgcgggtagt tatagttttt tcgtaatctt tactgaaggt
    22081 tgcgttgttg agtgctttga cctccttgcc aatagtaacg atcaaatcac gtttcggttc
    22141 gaggtggcgt aaacgtaact ctttaagatt atctgacagc tcccaggcac catccacttt
    22201 gccgcttttt ttatcgacga catgaataac gcgtgagaaa tcctgatccg ggtcgagagg
    22261 gatggagaac gtcagcacca gcgtggctgc accatcaagt tggacttcag agagatccag
    22321 caatgtcagc accttaccgg cactctgttg ggccagtttt tgtctctccg gcacggagag
    22381 ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta acttcagaag gcgcatcttt
    22441 tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct gccagcgcca gcattagcat
    22501 gcaggcggct acgcgtaact ttttcatttt tcatccctgc cacaatggcc cgttagcaac
    22561 gtcgaataat tattatgcgt gagaatttac gttatgtaat tcagtatcac gcccatacaa
    22621 tccgaatttt agtgagaatt gtggctcaga cagccaaatc agcctctttc gcttgtcgct
    22681 tttcactaaa catccgacaa tttagcctaa ccccggcaaa aatggagatg cctatgtcca
    22741 cgacatggtt tgtaggagcc gactggctcg ccgaacatat tgatgacccg gaaattcaga
    22801 ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg taacgttgct caggagtatc
    22861 tgaatggaca tattcccggc gcagtgtttt ttgatatcga agcgctttct gatcacactt
    22921 ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc cgtggcgatg cgtgaattag
    22981 gcgttaacca ggataagcac ctgattgtct atgacgaagg taatcttttc tcagccccac
    23041 gagcatggtg gatgctgcgc acctttggtg tagagaaagt gtcgattctg gggggtggac
    23101 ttgcaggctg gcagcgcgat gatctgctgt tagaagaagg tgcagtagag ctgccggaag
    23161 gagagtttaa cgccgcgttt aatcctgaag ccgtggtgaa agtaaccgat gtattattgg
    23221 caagccatga aaatacggcg caaattattg atgcccgccc ggctgcacgt tttaacgcag
    23281 aagttgatga acctcgccca ggtttacgtc gcggacatat tcccggagca ctgaatgttc
    23341 cgtggacgga actggtgcgc gaaggcgaac taaaaacgac cgatgaactg gatgcgatat
    23401 tttttggtcg cggcgtcagc tacgacaaac caattatcgt cagctgcggc tctggtgtaa
    23461 cggcagccgt ggttttgtta gcactcgcga cgctggatgt gccaaacgtg aaactgtacg
    23521 acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc ggttgagcca gtgaaataag
    23581 tattttacag gcaataaaaa accgccgaat ttggcggttt tttattgctg gtctggttcg
    23641 cggcctttcc agcaggttgt attaccgtag taatgcaagc gcgtctcagc ggagacaata
    23701 ctcgccagta actctctttt tgtcaagcaa aagagagtta ttattgttct gttagtgtat
    23761 tatccactgc ggccctttcc gccgtctcgc aagcgggcgc tggcttaagg aaaggatgtt
    23821 ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc gcggcaatat cgccagtggt
    23881 gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag atacggtgct tctgtatcgt
    23941 acttattgtt tctggtgcgc tgttttccca agtaaataat aaccggagtc tctccggcga
    24001 caatttactg gtggttaaca accttcagag cagcaagtaa gcccgaatgc cgccctttgg
    24061 ggcggcatat tttagattat ccgattctgt ttaaagtcac gcaaaaaacc accccagcga
    24121 cgttcataga atggcacaat atgttcggta ataaagtggc taattccttt ttcccctttt
    24181 ttcacctgac aaatatcgat tggttcatcg ccaggtaatg tatcggtcgc tacacttccc
    24241 gtcgcctgaa taatttcttc gatatcacca tcggcttcaa tgccaataag taaattaggc
    24301 tgtgcctctt cgttctcttt aattgaacaa ataaaagcac gcttcaccga cttaatggtt
    24361 ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcgac ttccgataat
    24421 atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc ctcttcacca
    24481 atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt ggcattaagg
    24541 aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac gacaaatgcc
    24601 tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg tgaaaaaagg aatgacgctg
    24661 gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcac tatcttcaac cacagcctcg
    24721 ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg gaaaaaggcc
    24781 gggcggtgcg ccggttcggt tgctgctttt tccagcaggt cttcaagttc gttttttgtt
    24841 tcggacatat gaaccacaat tcattcaacg ttcggggcgc aaatgcctga tgcgctacgc
    24901 ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc agaatttgta ggccggataa
    24961 ggcgtttacg ccgcatccgg catattagtt tacgccgtta acagattagc tatcgtgcgc
    25021 acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg gcgctttacg gtaagtcgcc
    25081 gagcagtcga tatgcagcca gccttgctga tagttctcaa caaagtgcga caggaagccc
    25141 gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg tattgttcag ttcggcaaag
    25201 ttagacggca gctggctgcg gtggaattcc gccagcggca gacgccagaa tggttcgttc
    25261 tcttgtgagg cactcgccag caaacgaccg gcaagcgcat cgtcaaaact gaacagcgcg
    25321 tgataatcat tacccagcgc agttttcgcc gccccggtga gggtcgccgc atcaatgatc
    25381 atttccggtt tctgcgcact ggcatcaatc agaccatcgg caagcaccag acgcccttcg
    25441 gcatcagtgt tcatcacttc aactttttta ccgttgcgat aggtgatgat atcgcccagc
    25501 ttgaacgcat tgccgctaat caggttatcc gcacagcaga ggaacagctt cacgcgcttg
    25561 ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa ccgttgccgc gccgcccatg
    25621 tccgacttca tcgagtccat aaacgcagtc tgtttgatgc tgtagccgcc ggagtcaaaa
    25681 gtgatacctt tacctaccag gcacgcgtac accggtgctt ctttatcgcc agttgggttg
    25741 taatccagcg ccagcaatac cggagaacgt tctgaaccgc gcccgacggt gtgcagcccc
    25801 atataacctt gctcacgcag atcttcgcct ttggtgatcc gataagtcac acgatcgccc
    25861 gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt gcgatggtcc caattcttct
    25921 gccggtgcgt tgatggtgtc acgcacccag tcgatgatca tcaggcggtt atccagttcc
    25981 tggcgctggg catcgtccag atccggccac tccactttac gcgtgccttt cggggctttg
    26041 taaccttgcc agaatgccca gcagcgatcc gcatcccagc cttcaccgct taactgaaca
    26101 tgcttgatgc ccagaccgtc aatcttgcgc gccgcacgct ggatcagccc cagatcgtct
    26161 gccccgttca aatgcagggt aatgccgtca ttattaatgc tgtaagttgc tttttctccc
    26221 cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca tcgcttctgt cattttagtt
    26281 atccttcttt gtaaaagggc cgccagttgg cagccccagg atttttccgc taaatgattc
    26341 gcgttgcagg aaggcggcaa gtgagtgaag ccccaggagc atagataact atgtgactgg
    26401 ggtgaacgag cgcagccaac gcatctgcgg cgtgaagcat gacgcggaaa ttattcggcc
    26461 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
    26521 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
    26581 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccat
    26641 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
    26701 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
    26761 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagcgagt
    26821 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
    26881 gttcaatctc gataccgtta cgcagagctg cgtcgagaat ggtttcaccg ctattagctt
    26941 ccagaacagc gccatcaggg cagagatcct gatgaggcaa aataacaatc tttggcatat
    27001 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
    27061 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaacg
    27121 tcatcgccct gcgccacttc actcaggtga gcggcagcat cgtcaatgac ctgacgttct
    27181 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
    27241 gccgcttcta ctttttgttc tgccagcatt cgggctttta cgtcctgctc ggcatagctc
    27301 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
    27361 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
    27421 gcatcgacct ggaacgtcac gcgaatatgc gcaccaccag ccggtagcgc cggaatacca
    27481 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
    27541 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
    27601 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
    27661 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
    27721 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
    27781 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
    27841 ccgcccacca tcaccacttc cagcacttca tcagcttcta cacccgcgtc tttcagcgcg
    27901 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
    27961 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
    28021 gcgattttgg ctgcaatggc ggcatccagc agttcacgct gaacgcggtt atcgctacga
    28081 tcaggaatgc ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
    28141 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
    28201 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
    28261 gaatccagcc cgtaggcgat agccgcagcg gtcggttcgt taagtaagcg cagaacatgc
    28321 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
    28381 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
    28441 agtgctttga ggatgtccgc agaaacgcgc accgggttca gcagccccgc cgccgtttca
    28501 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
    28561 tggatatcag ccagcgagcg tcccatcagg cgtttaacag aactaatggt gttggcggtg
    28621 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
    28681 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
    28741 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
    28801 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
    28861 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
    28921 gagaaaacgc agcttacgca cggtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
    28981 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
    29041 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
    29101 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
    29161 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
    29221 aatggttgca gattgctgta cggcggcgag ttgttccgcc tggcttccgc tggcgaattt
    29281 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag
    29341 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
    29401 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
    29461 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
    29521 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
    29581 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
    29641 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
    29701 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
    29761 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
    29821 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
    29881 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
    29941 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
    30001 agcgatagca gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
    30061 gttgacttta atctgcaact tcatcacgtc gccacaggcc ggtgccccca ccatgccgct
    30121 gccgacgttc tcgtcgttgt tgtcaaagga acccacgtta cgcggattct cgtaatggtc
    30181 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
    30241 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
    30301 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
    30361 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
    30421 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
    30481 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
    30541 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
    30601 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
    30661 ctcttctttt gcgatgcgat aggcctcgcc cattccgacg atctggtgaa caggcagagt
    30721 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacgcg
    30781 cggtttacga cgtacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
    30841 gaaagacatc aggtcaactt tcaactggct caggtcgata ggcagtttgc ccacgctctg
    30901 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
    30961 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
    31021 acgcatcgct gcttcaagtt ctttcaggtc gataatgccg ttacgctgcg gtgccaggta
    31081 ggtgacttca aaaccttcgc gctccagctg acggcaggta tccagtaccg ctttgtgttc
    31141 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
    31201 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
    31261 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
    31321 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcataa actgcatcat
    31381 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
    31441 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaaccaggct accggctgga
    31501 tgtacgaccg tgtttacgga gcgcgatacg ccccggcctg attctgaatt ctttttatta
    31561 agcgcgtaac ttaacgtcga tcgcgtcttg tatgcgggtg cgtggcgcgt cgtgagtatg
    31621 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
    31681 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
    31741 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
    31801 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
    31861 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
    31921 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
    31981 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
    32041 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
    32101 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
    32161 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
    32221 gttgtccttc cagaccatgg gcgggttgag ggcaattatt cgcattagcg ctggcctctt
    32281 cccctcaccc taaccctctc cccgaagggg cgaggggacc gtccgtgcat ttatctggct
    32341 tagccctcag gccttattac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
    32401 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
    32461 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
    32521 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
    32581 ctcgccgttt tcctgagttg ccagccaggc catgcgcact tcataggcaa taacctgaac
    32641 cgccatcgcc aggttcagcg agctgtattc cgggttagcc gcaatcgcga catgataatg
    32701 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
    32761 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
    32821 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
    32881 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgctgccag
    32941 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    33001 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    33061 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    33121 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    33181 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    33241 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    33301 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    33361 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    33421 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    33481 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    33541 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc
    33601 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    33661 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    33721 caaactgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    33781 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    33841 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    33901 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccacgtgttg tgaaagccat
    33961 gctggcgaac atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt
    34021 gatgtcactc acccgccctc gcctttcagg cgctattctg aaatacttcc tcaccgcttt
    34081 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    34141 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    34201 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    34261 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    34321 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    34381 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    34441 ggtcagttgg ttacccgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    34501 taaatcaaaa ggcacgccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    34561 tgtggtgcgc catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat
    34621 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    34681 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    34741 tggctactta cttgatgaga gttacagcgg cgaaaattat atcgccagcg tcagcccgat
    34801 cccgctttta ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa
    34861 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    34921 tgatgctttt tccgatcgcc acggcgatgt ttatcgcgaa cagatggtgg actttatcct
    34981 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    35041 caccttaaga catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac
    35101 gcttgccaac atttctgatg attagcattc ccttcgtcat ttccctgagc aaactttagc
    35161 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    35221 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    35281 tgaccgccac cacggcaact ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    35341 ttgatatcag cgaaacagga caggagatcc tgcgctatca tcaactcaca ctgacggctg
    35401 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    35461 actggctacg acgtggtttc cgtctgtgtc catcatttat taccagccat ttcacccccg
    35521 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    35581 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa acgcgcgata
    35641 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca acaccacgct ggaattacgc
    35701 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    35761 aggaaattgg acgccactgg cagcgtcggg cgctccagcc tgtaccacct gatgagccac
    35821 tgtttatggc actacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    35881 agcgagacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    35941 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatacc cacctcgccc
    36001 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    36061 ccagactgta cccacgcctg gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg
    36121 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    36181 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tcttcttacc gggaatgata
    36241 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    36301 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    36361 ttattgcgcc ttataccatg ccattaccgc tcttttcacc accgctgatc tatacggacc
    36421 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    36481 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    36541 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    36601 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgagca
    36661 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    36721 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    36781 caaatcgccg agatcaacag catatcgcgt gcactacaac ggcggaaaag tttattactc
    36841 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    36901 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    36961 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    37021 gctgaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    37081 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcgcgag gatcacccga
    37141 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    37201 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    37261 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    37321 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc
    37381 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    37441 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    37501 aaaccaatcc ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag
    37561 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    37621 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    37681 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    37741 acgcatgttg caaaaataat gcgtaattat gccgttacgt ttgccaaacg ttcctgcaca
    37801 atggcgatga aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa
    37861 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgtccggt attaaaatta
    37921 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    37981 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    38041 ttaacgatcg gagcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    38101 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacaca
    38161 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    38221 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    38281 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    38341 ggcagtaaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    38401 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    38461 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    38521 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    38581 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    38641 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    38701 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    38761 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    38821 accaactgat tgatacccaa aatggtcggg tcactccgcg tatttatacc gacccagata
    38881 tttaccaact ggagcttgaa cgtattttcg gccgttgctg gctatttctc gcccacgaaa
    38941 gccagatccc aaaacctggt gatttcttta acacctacat gggggaagat gcggttgtcg
    39001 tagtgcgtca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    39061 tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct
    39121 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    39181 aagggttgtg taaatcccac tggggactaa acgaagttcc ttgtgtggag agttataaag
    39241 ggctgatttt tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca
    39301 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    39361 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    39421 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    39481 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    39541 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    39601 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    39661 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    39721 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    39781 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    39841 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    39901 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    39961 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    40021 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    40081 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgctgct taccagcagg
    40141 aggtgatgaa atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct
    40201 ctttcacgaa gccagcttac tggacgactg gaaatttcgt gactggctgg cgcagctcga
    40261 cgaagagatt cgttacacca tgcgcaccac agttaacgcg caaacacgcg accgccgcaa
    40321 aggcgtccag ccaccgacaa cctggatttt taatgacacc aaagaccagc tggagcggcg
    40381 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcacccgtca
    40441 cttaatcagc aactgccaga taagcgaaac cgacatccca aacgtatttg ctgtgcgggt
    40501 aaattatctg ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    40561 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    40621 cctggatcaa gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    40681 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc
    40741 gtcatcgccc tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    40801 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    40861 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    40921 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    40981 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    41041 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca ggttgccacg ctggaactgt
    41101 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    41161 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    41221 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    41281 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    41341 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    41401 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccgcca
    41461 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    41521 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    41581 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    41641 cgctgcaatt tttcccgcaa ccggcggatt ttacgggtcc gtatgtgatg ttggcatcgc
    41701 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    41761 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    41821 ccatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    41881 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    41941 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    42001 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    42061 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    42121 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    42181 gctttaccct acgccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    42241 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    42301 agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac
    42361 caccgcccgt gcaacgctat cttttacagc gccaccagca ggctggtgtg cgcattctgc
    42421 tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg
    42481 gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac
    42541 tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca
    42601 cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg
    42661 cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg
    42721 caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca
    42781 gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc
    42841 cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca
    42901 cgctgaatca ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt
    42961 ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa ccaggataat
    43021 tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat
    43081 cacattatct tgcaaaggga ttggttatga acacactacg ttattttgat tttggagctg
    43141 cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc attatttttg
    43201 gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg ttgggcgcgc
    43261 caatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc gcgatattaa
    43321 tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac acgctgggta
    43381 cggcggtgat tggtcaccat tactgggata tgaccggcga tgcggttggg ccaaatctga
    43441 ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca attaccgggc
    43501 cgggggcaat ttctctcgat cggcgttagg aaagatgccg gatgcggcgt gaacgcctta
    43561 tccggcattt tataaattac agccactcta cccgcaacga catctctgag gttgttgatt
    43621 cccctggtgc tagcgcaatg agatcacccc cttccgggcg atgatgatca tccggcgcat
    43681 ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg
    43741 aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt
    43801 cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg ttcacccact
    43861 ggcgcggcaa cggcgcgagc tggttaaaat ccagttcctg cggtagctgc tcgcaaaact
    43921 cacccgccag ccactgctcc cgttccagcc agtaaccgct cgcctgtgcc tgaatccgcg
    43981 tttgcggcga caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc
    44041 cttgattggt gacagagagc gtcaccatca gcgtatccgc cgttaaatga aacgcctgac
    44101 ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa ctatcatcgc
    44161 tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc
    44221 actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacga ttgccgctca
    44281 cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca ccgctttttt
    44341 taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt
    44401 cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc cgctaactcc
    44461 ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca gcgcataggc
    44521 atcacctgcc tgttccagcg agaagcgatg ggtaatggcg ttacgcggcc acagcttcca
    44581 gtccgtcaga tcatgggcgc atttttccat atggaacaga ctggtcaccc aggagccgat
    44641 aatccgccgt tgatggtgca tcagatcggc gctgacctcg aattccactt ttccggtttc
    44701 accaatgtaa accacccgtc cccagtcagc agtggattgc agtgccagca agcgacctgc
    44761 ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt cagcaattat
    44821 ctgcggcaga ccttcggtgg tcgctaaata gccgtgatcc atcaccccta actgttttgc
    44881 catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac cgcgaccttt
    44941 cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca cgttatcgct
    45001 gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc aactgataaa
    45061 cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt cttccgccag
    45121 cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt
    45181 acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata
    45241 caccagcacg cggtcgccct ctttaaaatg gcggcagcct tgccccatcg ccacaatctg
    45301 cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg gtgccgccgc
    45361 cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc cggaggattt
    45421 cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc gcagatcgac
    45481 ggttgaattt cctggtaaat aagctgccag catcgttttc ataaatccct cattaacaat
    45541 acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct
    45601 ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat gatgacgccg
    45661 cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt accgattagc
    45721 gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc
    45781 gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg
    45841 ttaccagaac cgaggcgcgc cgccaacaaa atgccggtca ccgccgctaa taatcccgaa
    45901 agggtaaaga taagaatgcg cacccgacga acgttgatgc cgcacaactg cgccgccgta
    45961 gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg ttttgcggct gatgaacaca
    46021 aacagcgcaa acaacacaat catgatcagc gcggatactg gcacaccgag aaattgtccg
    46081 cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt ggtcataaac
    46141 agccccattc cgcgcagggc gctccacagc cccagtgtgg caacgaaact tggtacgtta
    46201 aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag caacaccagc
    46261 agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca caccgagaca
    46321 aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat cagcgtcatc
    46381 gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg gttattcaat
    46441 gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat caccaccagc
    46501 aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct cttgccctgc
    46561 ggcaatggta atgacgaagc agacatcttc aggttcctcg gtaaaattca tcagtgcaca
    46621 gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac
    46681 gtgccgtgct gtaataacag gatgcggtca cacaccagcg gcaactcctc cacttcactg
    46741 gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg gtaaatctgc
    46801 tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg
    46861 gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag cgtgccgatg
    46921 ggtgtttcgc tactggcagc cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg
    46981 cggatggtgg accattgcag cacaccgttg gtgctgattt tttgccgatt ggtcagcact
    47041 gtattttcgt caacgcccag ccagggaatg atccccgctt ctttgcggtt ttctggcgta
    47101 tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcatgatttt ctcgccgttg
    47161 ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag caattcactg
    47221 cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cgcgacgtaa cgtaaagctg
    47281 atatcctcca gcttgggctt atggcgtaat gcacggactt ccagcacggc ctgatcgaca
    47341 atttcctgag gggctaccgg cgcaatatca acgtgatcgc gccctagcat tagcgacaca
    47401 atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc atcgcgcata
    47461 acggtggcac aggaggcaat gcggcgaatt tcttccatcc ggtggctgac ataaatcacc
    47521 gccacgccca gtgccgacat ctttttcacc gcgctgatca ccagttcaac ttccgcactg
    47581 gcaagcgaac tcgtaggttc atcaagaatg accacgcgcg gctcgccctt catcacccgt
    47641 gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg ttcaggacta
    47701 acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg caggtaatca
    47761 atcatgccgt tgcggcgggg ccactgaccg aggcagaggt tttccgccac cgtcagccct
    47821 tccaccagac ttaactcctg ataaaccgca cgaaccccca gttcagcggc acggcgagtc
    47881 aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc gctatccggg
    47941 cgttcactgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt tttacctaac
    48001 agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc aacgacgccg
    48061 gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt tgccgtgaac
    48121 atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg ccagccactg
    48181 tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc
    48241 ggcttgttta ttgataacct tcaatgtttg cgcgagaaca gcattgccca ttttcttacc
    48301 ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag caatttcagt
    48361 ggtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt
    48421 taccgcgccg agtgtcgctc cgcccgattc acccataatg gcgttgagat ccggcgtgga
    48481 gataattagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctgattagc
    48541 gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg
    48601 cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt caattttatt
    48661 ggcaataaaa taatcggcgg cagcattacc cagtttttta ccaaattcca gcggatcgcc
    48721 gaccagatac gccgagacat atttatcgac acccttttga ttaatacagg tgttgtagca
    48781 aatcaccgga atacccgctt cactggcgcg acgaacggta cggctactgc cattttcaga
    48841 cactgccgat aaaataatgg catcgacatt acgcgccacg agggtatcaa caaaggtact
    48901 ttctttcgaa atatcaccct gggcgttagt ttcaattaac tgtacctgta ctgaagaatc
    48961 ttttgccgca tcctgaacgc cttgacgcac tccagcgtaa tatccctgag tatcaaggta
    49021 tattgcgcca atggtcattt ctttttccgc agccctggca aataaagcgc tacctaatag
    49081 cgtagccatt aataatagat tacgggttgt tctcattttt ttaggcatag agcctcctgt
    49141 agggttttta ttaacaacgg cttattctaa ttattttgtg atgagcggca gcgcgtgccg
    49201 ctcaaatatt acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt
    49261 ggtgaataat gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga
    49321 ctcatattgc gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt
    49381 ttctttatgc tgctgttgta aatcgccata cagcgacaac agatcaggtt gtgaaacagc
    49441 aaagaaatcc gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact
    49501 aaacagttgc tgtgcggtgt gttgttcgcc cagcagtcgc agcgccattc cttgccagaa
    49561 gagataatca accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt
    49621 cgccgccaga cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat
    49681 cgcctgccag aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt
    49741 ctccggataa tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct gtctggcatc
    49801 aaggtgctgc caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc
    49861 cccgccttcc cacgggtgga atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg
    49921 acccgtgaga tgccacaaat tgagcaactc tgctgtcatg tcgtcgcgtt tcagcgcaat
    49981 ttccagatta ttttccagac gcgccagtcg tttctccggt gtggctccgc ttaacttatc
    50041 aagcaaatcc cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc
    50101 aagataacgc gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc
    50161 gcgccagccg tcggcaaact ccggcgacat ctctacgcaa cgttgccata aagtaatggc
    50221 tttgttgtag ctgcgcttgt tgtagtagaa gcaggccagt aaatgacggg caaaccagca
    50281 ctcttcaata ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg
    50341 cgggaagaca tcaatggctt ttgcgaccag ttcgccgcgt tcggctttcg gcagcaagct
    50401 ggcttgtaga taaagcgata acgtgcgctg acagtccagt gcgttcagca tctctgtcgc
    50461 cagagtgggc attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg
    50521 tccctgacac agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt
    50581 cagccaccac agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg
    50641 cgcgttgtcc tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg
    50701 attggttggg caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc
    50761 attacgcgcc gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca
    50821 gaccgcacgc cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact
    50881 ggcgcgaatc aaactcgcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt
    50941 cagagcctga gtggcatacg ccaccgcttg cgggaaatcg gcacggttat attccagcat
    51001 cgccagcgcc aggttacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta
    51061 atcgaacggt gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc
    51121 ttcatctgta ctggtaatgt cttgtgctga cagtggcgct ttggcgacgt ccggcaacgg
    51181 caactcttgc ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag
    51241 ctcaatggtc agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg
    51301 catcagtgcc acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag
    51361 gcggtatccg ttcaacggag agatggcata cagcccccac tcaatccccc gctcactacg
    51421 ctggagtttt atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg
    51481 caggaaatac tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcaggctg
    51541 gttatcggca aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact
    51601 cttgtcccac gcctggccga attcactgtg tccccagctc cactgttttt tacctggcgc
    51661 gatatggtgg ttggcaacgt gtagcaaacc gccatcttca tcgtgacacc acgcgccaac
    51721 aaaatcgtac tgtgattttt cagccatata tgaggttgga acgggcacat ttttatagcg
    51781 agaaatgtcc actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa
    51841 agcggagacg gcccgtttgc cgtgatcaaa caccgccgtt acatccggcg ggaagacgct
    51901 ctgatgccct tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt
    51961 ggcgttgccg ttatagacgc ggctggcaat ttccaacgcc gcccgttcag gacgcagtgt
    52021 gaaacctgtc atcacctgta aaccgtgcat cggctccgtt tcgccgaccc acaccgtctg
    52081 tgcgccgtct tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg
    52141 gtgttgcggc cagttaaact caatcccgcc agagatccac ggtcccagca gccccaccag
    52201 cgcaggttta atgacttcat tgtgatagac aaaatcgcgt tgtttcactt tatcccatgc
    52261 gcgatgcaca cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag
    52321 ccacaccgcc tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta
    52381 gggataaacc gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat
    52441 atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg
    52501 agtcatggtg ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat
    52561 tgaacaatct ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg
    52621 aagatgagag cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg
    52681 gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt
    52741 tcgattccgg cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat
    52801 atcgacgatg aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa
    52861 ggtgactgga cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat
    52921 gcttgtttaa gtccgaaagg cgaatttgag tatttacaga ttgatgtacc gacgccgcag
    52981 gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc
    53041 accatcaatc tggcgctggc aatccacggt caggttgatc cagtgacagg agtgtcgcaa
    53101 accatgccgc aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaagagaag
    53161 ctgggcattc gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggaaaaatgg
    53221 caaaataatt cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg
    53281 tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acagattggt
    53341 cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa
    53401 accgtcgcct cgttaagcgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg
    53461 gttaatactc agcttgatcc tgaaaaattg actaccgcgc agttaatcgc tgcctggcag
    53521 agtggagaac cgtggatcac cagctgggtt gaccgcagtg ccaatgccat tggtttgagt
    53581 ctgtataact tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt
    53641 gcctttggtg agaactggct taatactatt attcgccaga cgggatttaa cccgttcgac
    53701 cgcgacgaag gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca
    53761 caacaggtgc tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg
    53821 cgcgataacg tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc
    53881 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    53941 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    54001 ttctgcttct ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt
    54061 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    54121 gcccagagcg gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa
    54181 caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgttccagga acacttctac
    54241 catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    54301 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    54361 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
    54421 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    54481 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    54541 gaacaggtaa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc
    54601 ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc
    54661 cagatcggcg tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
    54721 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    54781 acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    54841 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    54901 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
    54961 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    55021 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    55081 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    55141 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    55201 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    55261 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    55321 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    55381 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    55441 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    55501 atccctttac tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt
    55561 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    55621 cgtgaagcac tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg
    55681 ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa
    55741 cagtacaaca tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg
    55801 caggaagtgc tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat
    55861 cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc
    55921 gatttccgca ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag
    55981 ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg
    56041 aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat
    56101 ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg
    56161 cacaatcacg ccaatgttgg cgatgtcgtg aaactggttg ctccggcagg tgatttcttt
    56221 atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca
    56281 atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc
    56341 catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag
    56401 tcactgccgc gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct
    56461 aaaggtcagt ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc
    56521 gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa
    56581 cagttagtgg atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat
    56641 aaggtgctgt aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa
    56701 attgccgcgt cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg
    56761 aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag
    56821 gtatcgacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc
    56881 atatactccg cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact
    56941 tcggtcaccg tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc
    57001 ttgaagggtt ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt
    57061 cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc
    57121 agctcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc
    57181 gcgtgggtga cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac
    57241 tgattacgtg cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct
    57301 actaatgcat cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg
    57361 accaactggc gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg
    57421 gagaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc
    57481 agaagcggaa tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg
    57541 aggcggtaat agagatcttc acggaattcc ccgcgcgcca tcgcttttgg caggtcgcgg
    57601 tgagtggcgg aaataatccg cacgtcgata tcaatatcgc ggttgctgcc cagcgggcgc
    57661 actttacgct cctgcaacac gcgcagcagt ttgacctgca atggtgcggg catatcgcca
    57721 atctcatcga gaaatagcgt accgccttcc gccgcctgga ataaaccttc gcgattgctg
    57781 acagcgccag taaacgcgcc acgcgcatga ccaaacagct ccgactccag caactgctcg
    57841 ggcaatgcgc cacagttaat agcaataaat ggtttgctgt tacgcgggct ggcgttgtgg
    57901 atagcctggg cgaaaatctc tttcccggtg ccgctctggc cattaatcaa cacgctgacg
    57961 tctgattgcg ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg
    58021 acaattgcct cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca
    58081 attgcctgat atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc
    58141 tgtgttgcag caacggcatc ggggatagaa ccatgcgcag taagaataat taccggcatt
    58201 cccggctgca ctttctggat ttcagcaaac agctgcatac cgtccatttc atccatccgc
    58261 aggtcgctga taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca
    58321 ctttccgccg tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc
    58381 aatcccggat cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac
    58441 ctcatgggtg gatggcgcgg gcttttcacc ctcgtgcggc gtatccggcg agaaatttcc
    58501 agccggtttg cgggtcgaga gctggcgttc aatatcagtc aggttttcca gcttgcgggt
    58561 ggtgagttcc agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct
    58621 gtcgctggcc tgctggagct tgccgtagcg ttggcgctct tccgccagtt gcagttgcag
    58681 cgcctgaccg tcgcgccaga gttgatacag agggcgaacc tgtgccggga tctcggtact
    58741 tagcgcatca atgcgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc
    58801 gagcaaaatc ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg
    58861 ctgacgtgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag
    58921 cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag
    58981 atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt
    59041 attgtgactc tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg
    59101 tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaatg cctatcccag
    59161 tgggcgtaat tattgcaggc agtttggaca tggacagcgc ggagaaaccg gagcgtacac
    59221 gtcgtacgtg aggatttcga gcactgcccg gggccgaact ggcaaataaa atagcctgat
    59281 gggataggct gttagcatta tttcgtgttt ttcgacgacg gcaattcaat gcggaaacaa
    59341 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    59401 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttcct
    59461 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggtgt gcctgtattg
    59521 atgacatcaa tataaacccg tgcaccatgt aagctgctgc gaaggcaaat gttaccggat
    59581 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    59641 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    59701 gcgggcaggc tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc
    59761 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    59821 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    59881 ggcccgacaa cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    59941 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    60001 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    60061 gaaacgctat tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt
    60121 ttcaccggcc cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat
    60181 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    60241 tgcccacgag agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca
    60301 aaggcttcaa gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg
    60361 gccagattgt tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    60421 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    60481 gtaggatcgt ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    60541 gtcatcgctt cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga
    60601 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga
    60661 attagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac aggccagcgt
    60721 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    60781 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    60841 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    60901 tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    60961 agccactgct tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac
    61021 tgattatacg tcgctttgag gggtttatgg cttctccgtt atttgaatgt aaatcagcca
    61081 ctgtgtcgct aaaaagagac aacttaagat aaacttatta gataatatta aaatcaatga
    61141 gttaagtgtc gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa
    61201 aaaagctccc ggagttggga gcttatgata gtggttggtg cttaatgccg gatgcggcgt
    61261 aaatgccgca tccgggctgc aaaaccaatg aactgatgac ttaccccaac tgcttacgcg
    61321 cattgcggaa aatgcgcatc catgggccat cctcgcccca gttttccgga tgccaggagt
    61381 tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccacttt
    61441 cagtcgtaat cgacgtaata ccgttcggtg aaccgttcgg gttagccggg taggtttcag
    61501 tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg
    61561 ccagatgcgc cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca
    61621 tttgcgagcc caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaggc
    61681 tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac
    61741 ctgggatcag ttcgcgcagg ttagacatca tctggcaacc gttacatacc cccagcgcca
    61801 gcgtttgcgg acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga
    61861 ttgacttcgc ccaaccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg
    61921 cgaccagggc gtggaaatct tccaggcccg tgcgtccagt cagcaggtca ctcatatgca
    61981 catcgattgc atcgaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca
    62041 cgccctgctc acgcagcaca gcaactttcg gacgtgcgcc agtggcaata tacggtgctg
    62101 ccacatcttc gttgatatcg aacgacagtt tcacattcag gcccggatcg gcgtcgtttg
    62161 atttcgcctg gtgctcctgg tcagcacact ccgggttgtc acgcaggcgc tgcatctgcc
    62221 aggtagtttc tgcccaccag acacgcaacg tggtgcggct ttcgctgaat acagtctgcc
    62281 cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg ccctacataa tggacacaat
    62341 cagcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct
    62401 gaatcaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtcg cccagagagg
    62461 caatatccgc gttaatgccg caatgaccag caaaggccat ttccgccagc gttaccagca
    62521 ggccgccatc agagcggtcg tggtatgcga gcagcttacg ctgtgcaacc agcgcctgaa
    62581 tcgcgtcata gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa
    62641 gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat
    62701 caatcagcag cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat
    62761 cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt
    62821 cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca
    62881 ggcccagcgc cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat
    62941 cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat
    63001 cgccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg
    63061 cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt
    63121 agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggaccca
    63181 ccatctgatc gcgcgctacc atgccagtta cgctgcggtc accgatggtc accaggaaag
    63241 ttttctccgc cacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc
    63301 cttcacggac cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg
    63361 gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat
    63421 cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct
    63481 cacgcttaca cagttcgtca aacagcggta attgatcggc tgcaaccgcc agcacgtagc
    63541 gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa
    63601 tatcgcgcag ttcaaattta ccgccgcgtc cgccgtcgct caccagctcc ggcatcgcgt
    63661 tagaaagacc gcccgcaccg acgtcgtgga taaacagaat tgggttagca tcaccaagct
    63721 gccagcaacg gtcgatcact tcctggcaac gacgctccat ctccgggttg tcgcgctgta
    63781 cggaagcaaa gtcgaggtcg gcatcagact gacctgacgc catggaagac gccgctccgc
    63841 cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagcttcgca ccgacgttga
    63901 tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg
    63961 gtttgtgata accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac
    64021 ggaagtagcc attcagtgcc ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg
    64081 ggccttcggt catgatgtcc agcgcggtga caatacgctc aggcttaccg aaatcttctt
    64141 cccacggctg ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg
    64201 ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg
    64261 tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca
    64321 gaatatgcgc cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc
    64381 ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtaat
    64441 ctggcgtggt ttcgaaggtg tttttgatca tcttaaacag cgatttcggc tgttgctcac
    64501 cgtcgataac ccaatcggcg ttaaaaatct tgtggcggca gtgctcggag ttcgcctggg
    64561 cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca
    64621 gatagtcaat ttcatcttcc gccagtgcca ggccaagacg caggttagcg tcgattagcg
    64681 cctgacggcc ctgccccagc aaatcaacgc tggtgaccgg agtcggttga tggtgtgcaa
    64741 acagctgctc tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt
    64801 cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa
    64861 cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgggcaata tcggtcgctt
    64921 tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg
    64981 gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat
    65041 catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa
    65101 ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg
    65161 caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc
    65221 caaggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt
    65281 ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc
    65341 catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt
    65401 acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag
    65461 attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct ctggccatcc
    65521 attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg
    65581 cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc
    65641 ctggattacg aactggcgaa acagtttgcc gattacctcg gtgtaaaact gaaagtgacc
    65701 gtgcggcaga atatcagcca gctgtttgac gacctcgata atggtaacgc cgacctgctg
    65761 gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat
    65821 tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccgcg cacactgggc
    65881 aacctgacgg cggagcaact caccgttgca ccgggtcacg tggtggttaa cgatctccaa
    65941 accctgaaag acacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct
    66001 gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc
    66061 gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga tatcaccgat
    66121 gaacaaccgg tgacttggtt tagcccgtta gatggcgata atatcctttc cgccgccctg
    66181 ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga agagaaatac
    66241 ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat
    66301 gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt
    66361 ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac ttcaccgacg
    66421 ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg cattacggat
    66481 cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga tatgatgagt
    66541 aaagtgccgg aaagtgtgcc ggagaacgag cggatctggt ttgccctcgc tgcgtacaat
    66601 atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa agggaatcct
    66661 gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc
    66721 aagctgactt acggctacgc tcgtggacat gaagcctacg cttatgtcga aaatattcgt
    66781 aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc tacagaagcg
    66841 gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg caaagagaaa
    66901 tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca ttctccctct
    66961 ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga ggattgcgct
    67021 tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgcg
    67081 cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc cggatgatgc
    67141 agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg
    67201 cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc aagcgtgaca
    67261 tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg ccgcagggcc
    67321 atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca gccttcgccg
    67381 attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc atcccaggca
    67441 cgtttcgcca gcgtcagcgc gtgacgcatc cagtattcgt ggctaaattc gacttcagac
    67501 aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc
    67561 tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg acagaaataa
    67621 agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag cggagtgccg
    67681 attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt caatacccaa
    67741 ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag ccagggcgta
    67801 tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca
    67861 tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg
    67921 tcgcgaaacc agcgcacgaa atcggcctgc aatgtctgta aacgtgcttc gctgtgacca
    67981 aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat
    68041 aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag cggttggcga
    68101 cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt tccatctaag
    68161 tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat
    68221 cctgattaat tttcgctact acatagccta acagatagat catcactttt ccggcaacaa
    68281 tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc aatggaatat
    68341 aaaattcact cgcgtgtctc tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa
    68401 gatatgaacg gcttacttcg tatccgtcag cgttaccagg ggcttgccca gagtgataaa
    68461 aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa
    68521 ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc
    68581 tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag ccagccggaa
    68641 tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc
    68701 gaaaaactga ttaaagaaaa tactgccgct atgtacgcaa cgctaaacgt taatagtgaa
    68761 gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat tctgaccggt
    68821 attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc
    68881 aatgctgccg cagtgcgcga tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct
    68941 gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa cctggcggca
    69001 gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tccgaatgcc
    69061 ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagt
    69121 gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt cattgcgctg
    69181 attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa
    69241 aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag
    69301 tttccttatg gcgttgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga
    69361 atgcccgaat gaggcgattt caatgggaga tcatatctac gagattaaca gcgataagtg
    69421 taccgaatgc gtagggcact acgagacacc aacctgccag aaggtgtgcc cgatccccaa
    69481 tactattgtg aaagatccgg cgcatgtcga gacagaagaa cagttgtggg ataaatttgt
    69541 gctgatgcac cacgcggata aaatttaata ttctactctg gaagtagagt attaattata
    69601 ttactgggaa gccttaacgc cattatattt atttaattga tgacattagc ataatcattc
    69661 actaagttaa tttatatagt atctgccaag acacttattt atagttatta aaggcgcgtc
    69721 cgattggctc accggacgca ccttaagtac gtttccttgt gttataagaa cagaaggatc
    69781 agctgtaaaa cagcaatgat gattttgatg acc
//
LOCUS       DAFAVF010000006        66989 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFAVF010000006 DAFAVF010000000
VERSION     DAFAVF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.006"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..670)
                     /locus_tag="LGK42_000362"
     CDS             complement(<1..670)
                     /locus_tag="LGK42_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBK2656651.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="LGK42_000363"
     CDS             complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="LGK42_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBK2656652.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1245..3443
                     /gene="priA"
                     /locus_tag="LGK42_000364"
     CDS             1245..3443
                     /gene="priA"
                     /locus_tag="LGK42_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK2656653.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3599..4624
                     /gene="cytR"
                     /locus_tag="LGK42_000365"
     CDS             3599..4624
                     /gene="cytR"
                     /locus_tag="LGK42_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBK2656654.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4716..5675
                     /gene="ftsN"
                     /locus_tag="LGK42_000366"
     CDS             4716..5675
                     /gene="ftsN"
                     /locus_tag="LGK42_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBK2656655.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5768..6298
                     /gene="hslV"
                     /locus_tag="LGK42_000367"
     CDS             5768..6298
                     /gene="hslV"
                     /locus_tag="LGK42_000367"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBK2656656.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6308..7639
                     /gene="hslU"
                     /locus_tag="LGK42_000368"
     CDS             6308..7639
                     /gene="hslU"
                     /locus_tag="LGK42_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBK2656657.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7706..8632
                     /gene="menA"
                     /locus_tag="LGK42_000369"
     CDS             7706..8632
                     /gene="menA"
                     /locus_tag="LGK42_000369"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK2656658.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8725..9210
                     /gene="rraA"
                     /locus_tag="LGK42_000370"
     CDS             8725..9210
                     /gene="rraA"
                     /locus_tag="LGK42_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBK2656659.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="LGK42_000371"
     CDS             complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="LGK42_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBK2656660.1"
                     /translation="MTMSVEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9966..10811
                     /gene="glpF"
                     /locus_tag="LGK42_000372"
     CDS             9966..10811
                     /gene="glpF"
                     /locus_tag="LGK42_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBK2656661.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10834..12342
                     /gene="glpK"
                     /locus_tag="LGK42_000373"
     CDS             10834..12342
                     /gene="glpK"
                     /locus_tag="LGK42_000373"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK2656662.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12478..13488
                     /gene="glpX"
                     /locus_tag="LGK42_000374"
     CDS             12478..13488
                     /gene="glpX"
                     /locus_tag="LGK42_000374"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBK2656663.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13585..14331
                     /gene="fpr"
                     /locus_tag="LGK42_000375"
     CDS             13585..14331
                     /gene="fpr"
                     /locus_tag="LGK42_000375"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBK2656664.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14336..14764)
                     /gene="uspD"
                     /locus_tag="LGK42_000376"
     CDS             complement(14336..14764)
                     /gene="uspD"
                     /locus_tag="LGK42_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBK2656665.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14791..15090)
                     /locus_tag="LGK42_000377"
     CDS             complement(14791..15090)
                     /locus_tag="LGK42_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBK2656666.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15302..15742)
                     /locus_tag="LGK42_000378"
     CDS             complement(15302..15742)
                     /locus_tag="LGK42_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2656667.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15843..16442
                     /locus_tag="LGK42_000379"
     CDS             15843..16442
                     /locus_tag="LGK42_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBK2656668.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16550..17317
                     /gene="tpiA"
                     /locus_tag="LGK42_000380"
     CDS             16550..17317
                     /gene="tpiA"
                     /locus_tag="LGK42_000380"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK2656669.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17372..18127)
                     /gene="cdh"
                     /locus_tag="LGK42_000381"
     CDS             complement(17372..18127)
                     /gene="cdh"
                     /locus_tag="LGK42_000381"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBK2656670.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18234..19223)
                     /gene="sbp"
                     /locus_tag="LGK42_000382"
     CDS             complement(18234..19223)
                     /gene="sbp"
                     /locus_tag="LGK42_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBK2656671.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19543..20505)
                     /gene="pfkA"
                     /locus_tag="LGK42_000383"
     CDS             complement(19543..20505)
                     /gene="pfkA"
                     /locus_tag="LGK42_000383"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK2656672.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20686..21588)
                     /gene="fieF"
                     /locus_tag="LGK42_000384"
     CDS             complement(20686..21588)
                     /gene="fieF"
                     /locus_tag="LGK42_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBK2656673.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21737..22237)
                     /gene="cpxP"
                     /locus_tag="LGK42_000385"
     CDS             complement(21737..22237)
                     /gene="cpxP"
                     /locus_tag="LGK42_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBK2656674.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22387..23085
                     /gene="cpxR"
                     /locus_tag="LGK42_000386"
     CDS             22387..23085
                     /gene="cpxR"
                     /locus_tag="LGK42_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBK2656675.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23082..24455
                     /gene="cpxA"
                     /locus_tag="LGK42_000387"
     CDS             23082..24455
                     /gene="cpxA"
                     /locus_tag="LGK42_000387"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBK2656676.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24561..25235)
                     /gene="yiiM"
                     /locus_tag="LGK42_000388"
     CDS             complement(24561..25235)
                     /gene="yiiM"
                     /locus_tag="LGK42_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBK2656677.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25384..26367)
                     /gene="kdgT"
                     /locus_tag="LGK42_000389"
     CDS             complement(25384..26367)
                     /gene="kdgT"
                     /locus_tag="LGK42_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBK2656678.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26627..27247)
                     /gene="sodA"
                     /locus_tag="LGK42_000390"
     CDS             complement(26627..27247)
                     /gene="sodA"
                     /locus_tag="LGK42_000390"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBK2656679.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27532..28566
                     /gene="rhaT"
                     /locus_tag="LGK42_000391"
     CDS             27532..28566
                     /gene="rhaT"
                     /locus_tag="LGK42_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBK2656680.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWTYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAINAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28563..29429)
                     /gene="rhaR"
                     /locus_tag="LGK42_000392"
     CDS             complement(28563..29429)
                     /gene="rhaR"
                     /locus_tag="LGK42_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBK2656681.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(29485..30321)
                     /gene="rhaS"
                     /locus_tag="LGK42_000393"
     CDS             complement(29485..30321)
                     /gene="rhaS"
                     /locus_tag="LGK42_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBK2656682.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30609..32078
                     /gene="rhaB"
                     /locus_tag="LGK42_000394"
     CDS             30609..32078
                     /gene="rhaB"
                     /locus_tag="LGK42_000394"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBK2656683.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            32075..33334
                     /gene="rhaA"
                     /locus_tag="LGK42_000395"
     CDS             32075..33334
                     /gene="rhaA"
                     /locus_tag="LGK42_000395"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBK2656684.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33509..34333
                     /gene="rhaD"
                     /locus_tag="LGK42_000396"
     CDS             33509..34333
                     /gene="rhaD"
                     /locus_tag="LGK42_000396"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBK2656685.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34343..34657
                     /gene="rhaM"
                     /locus_tag="LGK42_000397"
     CDS             34343..34657
                     /gene="rhaM"
                     /locus_tag="LGK42_000397"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBK2656686.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34970..35416
                     /locus_tag="LGK42_000398"
     CDS             34970..35416
                     /locus_tag="LGK42_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK2656687.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLRRGDADQVFALLNQTLSS"
     gene            35427..36878
                     /locus_tag="LGK42_000399"
     CDS             35427..36878
                     /locus_tag="LGK42_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBK2656688.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36868..37938
                     /locus_tag="LGK42_000400"
     CDS             36868..37938
                     /locus_tag="LGK42_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2656689.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37938..39686
                     /locus_tag="LGK42_000401"
     CDS             37938..39686
                     /locus_tag="LGK42_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBK2656690.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIHEE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39736..40776)
                     /locus_tag="LGK42_000402"
     CDS             complement(39736..40776)
                     /locus_tag="LGK42_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBK2656691.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(40929..41762)
                     /gene="fdhD"
                     /locus_tag="LGK42_000403"
     CDS             complement(40929..41762)
                     /gene="fdhD"
                     /locus_tag="LGK42_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBK2656692.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            41956..45006
                     /gene="fdnG"
                     /locus_tag="LGK42_000404"
     CDS             41956..45006
                     /gene="fdnG"
                     /locus_tag="LGK42_000404"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:42541..42543,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBK2656693.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            45019..45921
                     /gene="fdoH"
                     /locus_tag="LGK42_000405"
     CDS             45019..45921
                     /gene="fdoH"
                     /locus_tag="LGK42_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBK2656694.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            45918..46553
                     /gene="fdoI"
                     /locus_tag="LGK42_000406"
     CDS             45918..46553
                     /gene="fdoI"
                     /locus_tag="LGK42_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBK2656695.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            46550..47479
                     /gene="fdhE"
                     /locus_tag="LGK42_000407"
     CDS             46550..47479
                     /gene="fdhE"
                     /locus_tag="LGK42_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBK2656696.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(47809..48051)
                     /locus_tag="LGK42_000408"
     CDS             complement(47809..48051)
                     /locus_tag="LGK42_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656697.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(48271..48489)
                     /locus_tag="LGK42_000409"
     CDS             complement(48271..48489)
                     /locus_tag="LGK42_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBK2656698.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            complement(49094..49384)
                     /locus_tag="LGK42_000410"
     CDS             complement(49094..49384)
                     /locus_tag="LGK42_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2656699.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(49385..49696)
                     /locus_tag="LGK42_000411"
     CDS             complement(49385..49696)
                     /locus_tag="LGK42_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2656700.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            49925..50833
                     /locus_tag="LGK42_000412"
     CDS             49925..50833
                     /locus_tag="LGK42_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2656701.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(50897..51838)
                     /gene="fabY"
                     /locus_tag="LGK42_000413"
                     /gene_synonym="yiiD"
     CDS             complement(50897..51838)
                     /gene="fabY"
                     /locus_tag="LGK42_000413"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBK2656702.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(51883..52320)
                     /gene="dtd"
                     /locus_tag="LGK42_000414"
     CDS             complement(51883..52320)
                     /gene="dtd"
                     /locus_tag="LGK42_000414"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK2656703.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(52317..53189)
                     /locus_tag="LGK42_000415"
     CDS             complement(52317..53189)
                     /locus_tag="LGK42_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBK2656704.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(53183..53782)
                     /gene="yihX"
                     /locus_tag="LGK42_000416"
     CDS             complement(53183..53782)
                     /gene="yihX"
                     /locus_tag="LGK42_000416"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBK2656705.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(53881..54666)
                     /locus_tag="LGK42_000417"
     CDS             complement(53881..54666)
                     /locus_tag="LGK42_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2656706.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(54700..55596)
                     /gene="yihV"
                     /locus_tag="LGK42_000418"
     CDS             complement(54700..55596)
                     /gene="yihV"
                     /locus_tag="LGK42_000418"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBK2656707.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            55764..56660
                     /gene="yihU"
                     /locus_tag="LGK42_000419"
     CDS             55764..56660
                     /gene="yihU"
                     /locus_tag="LGK42_000419"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBK2656708.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            56684..57562
                     /gene="yihT"
                     /locus_tag="LGK42_000420"
     CDS             56684..57562
                     /gene="yihT"
                     /locus_tag="LGK42_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBK2656709.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            57579..58820
                     /gene="yihS"
                     /locus_tag="LGK42_000421"
     CDS             57579..58820
                     /gene="yihS"
                     /locus_tag="LGK42_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBK2656710.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            58958..59860
                     /locus_tag="LGK42_000422"
     CDS             58958..59860
                     /locus_tag="LGK42_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBK2656711.1"
                     /translation="MQITNMHCSGQTVSLTAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            60059..62095
                     /gene="yihQ"
                     /locus_tag="LGK42_000423"
     CDS             60059..62095
                     /gene="yihQ"
                     /locus_tag="LGK42_000423"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBK2656712.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            62141..63526
                     /locus_tag="LGK42_000424"
     CDS             62141..63526
                     /locus_tag="LGK42_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656713.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            63569..64972
                     /locus_tag="LGK42_000425"
     CDS             63569..64972
                     /locus_tag="LGK42_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656714.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            65040..65732
                     /gene="ompL"
                     /locus_tag="LGK42_000426"
     CDS             65040..65732
                     /gene="ompL"
                     /locus_tag="LGK42_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBK2656715.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(65823..>66989)
                     /locus_tag="LGK42_000427"
     CDS             complement(65823..>66989)
                     /locus_tag="LGK42_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656716.1"
                     /translation="MQEYFHLTNGQIGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMS
                     FSLIATGLLGVYLTTMPGYWGILFVWALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLF
                     GFFETGRGIVDTVVAFSALAVFTWFGSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKE
                     EAPSVEVKKEDGASKNTSMTSVLKDKTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLL
                     PVALVGAYGIINQYCLKMIGGPIGGMISDKILKSPSKYLCYTFIISTAALVLLIMLPH
                     ESMPVYLGMACTLGFGAIVFTQRAVFFAPIGEAKIAENKTGAAMALGSFIGYAPAMFC
                     FSLYGYILDLNPGIIGYKIVFGIMACFAFCGAVVSVMLVKRISQRKKEMLAAEA"
BASE COUNT        16398 a        17416 c        17071 g        16104 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagtaccc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggactcagg cgaagctctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacgccgc
      241 cgcgcaccag ccacagtgac tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccgatg cgcaccccgg
      601 ctgaaccctg gacaatgcta ccgccatact gcgtcatgtc gccctgacgc gctgccggtt
      661 ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa
      721 caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat
      781 tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
      841 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
      901 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
      961 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     1021 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     1081 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
     1141 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     1201 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
     1261 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
     1321 ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg
     1381 tggtgtcagt tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagttgaag
     1441 tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc tgggcggcag
     1501 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc
     1561 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggtcagg
     1621 cggtggatct taacagcctg aaacgttcgc caaagcaaca acaggcgctg gcggcgttac
     1681 ggcaaggcaa aatctggcgc gaccaggtcg cggaacttga atttaatgat gccgcgttgc
     1741 aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
     1801 ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caagccaccg
     1861 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
     1921 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     1981 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
     2041 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcggc
     2101 tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtgatcggc acccgctccg
     2161 cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag cacgacagct
     2221 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc
     2281 atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttatgca
     2341 acgtccagca gaaaaaatac cgcctgctgc gcctgacccg tcgggcaggg aatgcgcgtc
     2401 cggcaattca acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg
     2461 cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc ctctttctta
     2521 atcgtcgggg ttttgcgcct gcgctgctgt gccacgactg cggctggata gccgaatgcc
     2581 cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact
     2641 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtcc
     2701 ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgatgttc cccggcgtgc
     2761 ccatttcacg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
     2821 cggaagtgca tcgcggcggc gcgaggatct tgattggcac gcaaatgctg gcgaaaggtc
     2881 accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg
     2941 ccgattttcg ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg
     3001 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt
     3061 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgcta gctgagcgac
     3121 gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     3181 atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg
     3241 cagacgacaa actgtgggtt ctcggcccgg ttccggctct ggcacctaag cgtggtggcc
     3301 gctggcgctg gcagatcttg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
     3361 gtggtacgct ggcgcttatt aatacaatac cggattcccg caaggtgaaa tgggtgttgg
     3421 atgtcgatcc catcgaaggc taatgcgcaa acgttgcgag gcggatcgaa aaattcaata
     3481 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     3541 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     3601 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     3661 ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     3721 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcaaccta tggggcgcaa
     3781 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
     3841 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     3901 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     3961 caagcaaatt gatggcatgt tgctgctggg ctcaaggctg ccgtttgatg ccagcattga
     4021 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg aactggagct
     4081 gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
     4141 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccac tgtgtcacta
     4201 tcgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     4261 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
     4321 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     4381 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     4441 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     4501 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     4561 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     4621 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     4681 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     4741 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     4801 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     4861 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     4921 aagtgaccgg taacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     4981 tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     5041 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     5101 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     5161 gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg
     5221 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     5281 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg
     5341 atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg
     5401 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
     5461 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
     5521 cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
     5581 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
     5641 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
     5701 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
     5761 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
     5821 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
     5881 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
     5941 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
     6001 gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
     6061 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
     6121 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gtgcactgtt
     6181 agaaaatact gaactgagtg ctcgtgaaat tgctgaaaag gcgttggata ttgcaggcga
     6241 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
     6301 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
     6361 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
     6421 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
     6481 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
     6541 catcaaagtt gaagcgacca aattcaccga agtaggctac gtcggtaagg aagtggattc
     6601 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
     6661 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
     6721 tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
     6781 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
     6841 cgcagcaccg atgggcgttg aaattatggc tcctccaggc atggaagaga tgaccagcca
     6901 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
     6961 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
     7021 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
     7081 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
     7141 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
     7201 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
     7261 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc tgaccaccag
     7321 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
     7381 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga
     7441 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
     7501 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaactat
     7561 catcattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
     7621 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
     7681 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
     7741 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
     7801 agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactgattac
     7861 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
     7921 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
     7981 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc
     8041 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
     8101 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
     8161 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
     8221 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
     8281 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
     8341 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
     8401 gctgatgggc tcactggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
     8461 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
     8521 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
     8581 gactaacctg ctgtttattt tagggatatt cctaagccag tgggcagcat aactgacaaa
     8641 tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc
     8701 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
     8761 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
     8821 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
     8881 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
     8941 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
     9001 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
     9061 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
     9121 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
     9181 ctttcagaag atccgctgga tattgaatga tagaaaaggc accgcaaggt gccttttttc
     9241 tgcgttacct gttggcctac acagtaaaga aattacgcga aagatgaagc gtaatcagac
     9301 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
     9361 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
     9421 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
     9481 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctactg acattgtcat
     9541 acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat taccggtgac
     9601 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
     9661 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac
     9721 ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg
     9781 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
     9841 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
     9901 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
     9961 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
    10021 ggttgttgat tttcttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
    10081 ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga
    10141 ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg
    10201 cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct
    10261 gtgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
    10321 atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc tctacttacc
    10381 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
    10441 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
    10501 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacag
    10561 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
    10621 ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg ccgcttttcg
    10681 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
    10741 tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca gaacaaaaag
    10801 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    10861 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    10921 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggttgggt agaacacgac
    10981 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    11041 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    11101 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    11161 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    11221 ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg
    11281 gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    11341 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    11401 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    11461 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtatacgg tcagactaac
    11521 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc
    11581 gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc
    11641 tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc
    11701 accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg
    11761 gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat
    11821 tccgaatatt tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggcattt
    11881 accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact
    11941 cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg
    12001 cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg
    12061 gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtccgatat tctcggcacc
    12121 cgcgttgagc gcccggaagt tcgcgaagtg actgcattgg gtgcggccta tcttgctggt
    12181 ctggcggtcg gtttctggca gaacctcgac gagctgcaag agaaagcggt gattgaacgc
    12241 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    12301 gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag
    12361 cgtttatgcc gcatccggta gtcctgaaac cgtgcggggg caaccccgca cacatcaata
    12421 atcccaccct tcccctgtgc tacacttcgc gccattcctt actgcttaga gtttgctatg
    12481 agacgagaac ttgccatcga attttcccgc gtcaccgaat ctgcggcgct ggctggctac
    12541 aaatggttag gacgcggcga taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt
    12601 attatgctca accaggtcaa cattgacggc accatcgtca ttggtgaagg tgaaatcgac
    12661 gaagcaccga tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat
    12721 attgctgttg atccgattga aggcacgcgc atgacggcga tgggccaggc taacgcgctg
    12781 gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg cgccggatat gtatatggag
    12841 aagctgattg tcgggccggg agccaaaggc accattgatc tgaacctgcc gctggcggat
    12901 aacctgcgca atgtagcggc ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt
    12961 ctggctaaac cacgccacga tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta
    13021 tttgctattc cggatggcga cgttgcggcc tcaattctca cctgtatgcc agacagcgaa
    13081 gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg tagtttctgc ggcggtgatc
    13141 cgcgcattag atggcgacat gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac
    13201 aacgaagaga atcgtcgcat tggcgagcag gagctggcac gctgcaaagc gatgggcatc
    13261 gaagccggta aagtattgcg cctgggcgat atggcgcgca gcgataacgt catcttctct
    13321 gccaccggta ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg caatatcgcg
    13381 actaccgaaa cgctgctgat ccgcggcaag tcacgcacca ttcgccgcat tcagtccatc
    13441 cactatctgg atcgcaaaga cccggaaatg caggtgcaca tcctctgatt gatttgatcg
    13501 attgagcctt ccagtccttc gggactggaa tttttttgtt cggagaacga agataaggca
    13561 agttaatcaa aacaggagaa aaacatggct gattgggtaa caggcaaagt cactaaagtg
    13621 cagaactgga ccgacgccct gtttagtctc accgttcacg cccccgtgct tccgtttacc
    13681 gccgggcaat tcaccaagct tggccttgaa atcgacggcg aacgcgtcca gcgcgcctac
    13741 tcctatgtta actcgcccga taatcccgat ctggagtttt acctggtcac cgtccccgat
    13801 ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg atgaagtgca ggtggttagc
    13861 gaagcagctg gcttctttgt tctggatgaa gtaccggact gcgaaacgct atggatgctg
    13921 gcaaccggta cagcgattgg cccttattta tcgattctgc aactaggcaa agatttagat
    13981 cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg ccgccgactt aagctatttg
    14041 ccactgatgc aagaactgga aaaacgctac gaagggaaac tgcgcattca gacggtggtc
    14101 agtcgggaaa cggcagcggg gtcgctcacc gggcggatac cggcgttaat tgaaagtggg
    14161 gaactggaaa gcgcgattgg cctgccgatg aataaagaaa ccagccatgt gatgctgtgc
    14221 ggcaatccac agatggtgcg cgatacacaa cagttgctga aagagacccg gcagatgacg
    14281 aaacatttac gtcgccgacc gggccatatg acagcggagc attactggta agcggttact
    14341 tatcgataaa cggcacgatg agcaaatccg cactcatctt attgatcatc ccgcgatatg
    14401 ccggcatcaa acggttgata aatgagtgat gatgaccaca gacaaggagg tcgcactgct
    14461 ctttttgcat aatttccagc agtgtttccg gcatttctcc gcgttcaata cgcagttttg
    14521 tcttcggcca ttgaatattt ttcgtcagtt tatacagctt gttatccgac ttattcttca
    14581 acaattgaag aatatcttct gttgcaggga agtagatacc cggatacaac tcgcttaagc
    14641 catcatcaat atgaattaac gtcaggtgag cgtcattatg tctggcgagc tccagagctt
    14701 tattcaccag taaggcatct tcttcattcc cggaaattgc cacgccaatg tgtttataag
    14761 ccatgtttaa ctccttctaa agcaactcca tcaagctaac gaacgcagtg ataactcaaa
    14821 aataatcatc tcggcctggc atgagaaagt gaaggccgca tcaagctcaa cttgcccctc
    14881 cagatcctta atcttgtagg ccacgctggc cccttcatct gcagaggatg cagcggcggc
    14941 aagctctttc agtttggtca taaaagactc tgcttcttcg cgggtggcaa aaaagcgcga
    15001 aaacttactg gtacagttgt cgttatcaat caccgtaccg atatcaatcg cacatccttt
    15061 agtactgcat ttatctacga catctttcat agggcacctc tgtatattcg cctctctgtc
    15121 gattacgact taagaataat gcgccgttaa gggaagaaca aacaagtaat gatcttatgg
    15181 attaccattt tttttgatat tgcttaaggt gaagagttca gaaggatttt ccgggcattg
    15241 tcatttgcat cgtggaaata acaatcccgg aaaaaaatag cagtaagaga tatattaaag
    15301 gttaattact tgatttattg tcggctttat acttcacatc ctgggtatct ttaccatatt
    15361 tattttcgcc ttgtgtgcca atgaacgctc caagatcgat aagcatcatc accagaatca
    15421 acgtcgggac aaaacgcccc accgcccatt gccagacacc cggtaaaatc gcccagttac
    15481 ccgccagcag catccacgcc acaatcatca gaaatgccca tgcgccggaa cgcccgcgat
    15541 catgcaagcg cttaacggtt actgccgctg ttggccaaag caagcacacc aggcaaaacg
    15601 ccgcggtctg aatatcgagt aaatttttac ccgccagtga gaaaagcacc agcatgcctg
    15661 ctaaccacag gcctatccaa atccagaaat cacggcgtcc aatacgccct ttaaatgaga
    15721 ataaccattg ctgtatggtc atgtaagttc cttgatggtt gtcttttcca ggattctacc
    15781 cttttgacaa gggcgacaga tatcgtttta atcggagcca gtcataacaa aaggtactgt
    15841 caatgaagcc agggtgtacg ctgttttttc tcttatgttc tgcattaacc gttacaacaa
    15901 cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc ttatctgctg gctggagccc
    15961 ctactttcga tctctccatc agccagtttc gagaagactt taacagccag aatcccagcc
    16021 tgccactgaa cgaatttcgt gccatcgaca gcagtcccga caaagccaat ctcactcgtg
    16081 ctgcaagtaa aattaacgag aacttgtatg cttctacagc gctggagcgc ggtaccttaa
    16141 aaatcaaaag cattcaaatg acctggctac ccatccaggg gccagagcaa aaagcagcga
    16201 aagcgaaagc tcaggaatac atggcagcag tgatccgcac actcacccca ttaatgacca
    16261 aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt
    16321 acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga
    16381 ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt ttgaataaat
    16441 gacaaaaagc aaagcctttg tgccgatgaa tctctatact gtttcacaga cctgctgccc
    16501 tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc
    16561 tttagtgatg ggtaactgga aactgaacgg cagccgccac atggttcacg agctggtttc
    16621 taacctgcgt aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg caccaccgga
    16681 aatgtacatc gatatggcga agcgcgaagc tgaaggcagc cacatcatgc tgggtgcgca
    16741 aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa acttctgccg ctatgctgaa
    16801 agacatcggc gctcagtaca tcatcatcgg ccactctgaa cgtcgtactt accacaaaga
    16861 gtctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa gagcagggcc tgactccggt
    16921 tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc aaaactgaag aagtttgcgc
    16981 acgtcagatc gacgcggtac tgaaaactca gggtgctgcg gcattcgaag gtgcggttat
    17041 cgcttacgaa cctgtatggg caatcggtac tggcaaatct gcaactccgg ctcaggcaca
    17101 ggctgttcac aaattcatcc gtgaccacat cgctaaagtt gacgctaaca tcgctgaaca
    17161 agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca
    17221 gccagatatc gacggcgcgc tggttggcgg tgcttctctg aaagctgacg ctttcgcagt
    17281 gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc cggatttcat
    17341 atccggcact tactttcctt aactcttcgc cttaacgcaa aatctcacac tgatgatcct
    17401 gaatttcctc ggctgaagca cggttaagcg tcagtagatt tcgttgtgtc gccagcaata
    17461 caaatgagtt atcactctgc cgtaccatcg ccagcccgta gcttcccata tgttcccgcg
    17521 cctcaggtac ttcttctgcc agcatcataa atgggctgcg ttgtaccagt tcgctttccg
    17581 ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc tggcagtggc aaccagcggc
    17641 tgctgatgtt cgccagattg ttatccagct gttcgcgcac atcaggacga atacaagaga
    17701 tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc aaagaaaccg
    17761 cgcgatcggg aaccggctgg ccgtattttt tgctcataaa atcacgcgcc tgccaggcca
    17821 accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg actttcagta ccgttaatac
    17881 gatacgttgg catcaacaga tattgcagtg gaccattaag atcttttaaa accacgtatc
    17941 cggcattggg tttgacttcc gcacatggcg aaggattttg attttgctgc tgattgggca
    18001 aacattcctc aaggacaatc ttacgtaatg tatccgactc ttcaccggtt aatttccagt
    18061 aaccaatacc ggcagccaca acggcgataa ctatcatcac caaaaaaaga agacccgctt
    18121 ttttcatctt tttttccctg tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa
    18181 ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat tatcctgggg aaatcagcgt
    18241 ttgctgatct gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc tttcgtccag
    18301 ccgccgaact cttcatcaat ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt
    18361 ttcgctactt cagcgtcgcg cggacggtag tagtttttcg cggcaatttc ctgaccttct
    18421 ggcgagtaga gatatttcag gtaggcttcc gccacctctt tggtgccttt tttctcgacc
    18481 actttatcga ccaccgacac ggttggctct gcgaggatag actcactcgg cgtgacgatt
    18541 tcgaatttat ctttccccag ttcattcgct gccagcagag cttcgttttc ccaggcgatc
    18601 agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg agccgcgcgc gccagaatcc
    18661 agaacttcga cgtttttata cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg
    18721 ttgttgtgat gcagcgcgta gccccaggca gccaggtagt tccagcgtgc gccaccagag
    18781 cttttcggat taggcgtgat caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg
    18841 atctgcttcg gattgccctt acgcaccagg aaaacaatgg tggaagtgta cggtgcggag
    18901 ttatccggca gacgtttgat ccactcttta tcaatccgcc cgcgttccgc aatcgcgtcc
    18961 acgtcatagg ccagagccag cgtgacaaca tcagcttcaa taccgttgat taccgacgtc
    19021 gcttgtttac ctgaaccacc gtgtgactga cgaatcacca cgttatcacc agtttgctgt
    19081 ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt tggatcatat
    19141 gaaacgttaa gaagctgaat atcctttgcc ataacgctgg ttgccgccag caaaaatgtt
    19201 aaccctacgc cccacttgtt catcgcccga ctctcttatg ttgtgttgtg atgagcaaag
    19261 cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag attggctata acttgcgggt
    19321 atatgttgag ggattaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct
    19381 ttatcgatgc cggatgcggc gcgaacgcct tatccgacct acaaaaattt gtaaattcaa
    19441 taaattgcag aattcacgta ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt
    19501 catcggtttc aggctaaagg aatctgcctt tttccgaaat cattaataca gttttttcgc
    19561 gcagtccagc cagtcacctt tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc
    19621 gtggtgaacc agctgttcgt tctggatacc tacacaacga ccgccgtaac ctgccagcag
    19681 cagatcgata gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga
    19741 accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc
    19801 gatgaaatgc gccagttcgt caacatcaca catatgttcg gtaatcgcca cgatcgcgtg
    19861 ttttttacct ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac ggctgaattc
    19921 aacttccgga actacaacga attcacagcc accggcaatg gccgcagcca gggtcagatc
    19981 gccacaataa cgacccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc
    20041 acgcagacgg tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta
    20101 gtcagtgcct ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat
    20161 ttcggtcaga cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcgtc
    20221 gataccacgt tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc
    20281 cgggaagcgc gcagaaccga ggaacgtacc gccacggttg atcatgtcag aaacgctgta
    20341 acggtctagc tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatgcccat
    20401 tacttccaga ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc
    20461 tggcgcatca ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact
    20521 ttggaatgca aaataaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg
    20581 aatgtgcaaa tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc
    20641 ggtattttat acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga
    20701 ccgtttaccc tccctgggta cgacggaaca ggggtcctga tggataatta catccgatcc
    20761 cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa
    20821 aggcagagag tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg
    20881 cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc
    20941 ttgccgttcc tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct catatcccat
    21001 gcgtaacgcg ctatataaaa tatagatgcc gattcccaat gcaaacagag catcggcgcg
    21061 atgccagccg taccaggaca accccagcgc cagcagaatt gcgccgttca tcataacatc
    21121 agactggtaa tgtagcatat cagcccgcac cgcctggctt tgcgtccggc gcaccaccca
    21181 acgctgaaac gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc
    21241 gacgcctgga tctgtcatcg gtgttggaga tatcagatgt tgaatacccg tcaaaaacag
    21301 gaatagtgcc gaaccggaga taaacatact ttgcgccagc gccgcgaggg actctgcttt
    21361 accgtgacca aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa
    21421 attcgtcaac gacgcgccga tatccaccag cgaatccacc agcgcggcga gaatactcac
    21481 cgacccggta taccaccatg caaaaatttt aatcagcaat agcaacgaag ccatcgccgt
    21541 cgcagcaatc gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc
    21601 tatcaactga cgctagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa
    21661 attgaggata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca
    21721 aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg
    21781 aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga tgtttctcgt
    21841 ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca
    21901 tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt
    21961 tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa
    22021 caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt
    22081 cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac
    22141 ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca
    22201 gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg
    22261 tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa
    22321 aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt
    22381 taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag
    22441 gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt
    22501 gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc gaagaaaaat
    22561 ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg
    22621 gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc
    22681 ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg
    22741 cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc
    22801 ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc
    22861 ggtactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt
    22921 gaacatttaa gccaggaagt gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat
    22981 atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt
    23041 aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg
    23101 cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt
    23161 tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcaggggc
    23221 tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt
    23281 ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat
    23341 tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta
    23401 actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg
    23461 aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc
    23521 gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac
    23581 tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg
    23641 caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc
    23701 agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc agttttaacc
    23761 agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct
    23821 ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc
    23881 gtagtggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca
    23941 tgatcaacga tctgttggtg atgtcccgta atcagcaaaa aaacgcgctg gttagcgaaa
    24001 ccatcaaagc caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc
    24061 agatgggcaa gtcgttgacg gttaacttcc cgcctgggcc gtggccgctg tacggcaatc
    24121 cgaacgcact ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata
    24181 cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg
    24241 atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg
    24301 atgaagcacg cgatcgtgaa tctggcggta caggattggg gctggcgatt gttgaaaccg
    24361 ccattcagca gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc
    24421 tggtgatttg gttgccgctg tataagcgga gttaaactcc gcatttgtag gcaggataag
    24481 gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga tagcgtctta
    24541 tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc ttcgatcttg
    24601 ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc ggcggcggag
    24661 agtaaacggt gatattgatc gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc
    24721 tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc agacactttc
    24781 cctggcgcta tcacgctata caaccacccc accttacctg tgttttgcat caactgcgct
    24841 atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc
    24901 tgaattaatg cctctcccca gcggaaaata tcgcccatat aaacattact ttccgtgaga
    24961 ccgtcggttg agagattttc gccaaacgca ggcgcaacaa acaactccgc ctgttccgga
    25021 aattcccgcg cccagtagag ataatgctca cgaggataat gacacagcgc tctgtctggc
    25081 ccgccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc cgtcagcatc
    25141 aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg ataagcctga
    25201 atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccccctccac gagaaatttc
    25261 cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca
    25321 ggaactcaat agcaaaccgg acgaacgtta gttcatccgg ctgtggggat taaggaagca
    25381 ggtttatttc accgtaccta aaatttcgat tttcgctgct ctggctttga ctttacgtga
    25441 ccagatagag gtaagaattg gcaccagaat cgaagtcaca atgaccgccg tcgctaccag
    25501 cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg
    25561 tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc catcgccacc
    25621 gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta ctgccacacc
    25681 caacaggatc cccagcaaac cagtctgggc aatcacagtc aaatcaatgg tattgcccag
    25741 cgcgaaggca aagaatggaa tcagcgtttg caccgctttg ctgaaaaatt ctcgtaattc
    25801 agggtcaagg ttccccaggg caaagcccac caggaacggt aatactgcgc cgacgaaaac
    25861 atgcggttca aacgaggcaa tccctgcagt gcccagaata atcatcgtca tgagcggccc
    25921 ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt actgctgcat
    25981 gatggaagcg taaagtccgc cattggtcat atccatcgcc gccaccagcg ccagcgttga
    26041 aagtccggca aagaatccaa cttcaacacc atgttccgga ataatgcgcg aggcaattgc
    26101 cgcaaccacc cacgcgacgg caatttttgt taccaccagc gtaccggatt tacgcaatac
    26161 cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat
    26221 gggcaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc ccgcccccgg
    26281 cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc cccccgggat
    26341 tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg
    26401 atgcgtcatt cctcaatgga gcatcagtgt tgatggaaac gaagatagac attcatttaa
    26461 tcaataaaag tgatcaaaac cacatcaatt gaaacactgt tttattttta tcggatcatt
    26521 gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta
    26581 agctgatatg cggcctatac gcctcattgc agcaggcggc aaatgattat tttttcgccg
    26641 caaaacgtgc cgctgcttcg tcccagttca ccacgttcca gaactcttta atgtagtccg
    26701 gacggcggtt ctggaatttc aggtagtaag catgttccca cacatccagg cccagaatcg
    26761 ggaagccgga agcgccagaa atagcttcac ccatcagcgg agaatcctgg ttagcagtag
    26821 aaaccaccgc cagtttatcg cctttcagca ccagccatgc ccagccggaa ccaaagcggg
    26881 aagctgccgc tttttcaaat tctgctttga agttatcaac ggagccgaag tcacgttcga
    26941 tagccgcttt caggtcaccc tgcagggtgg tgcctttttt cagacctttc cagaacaggc
    27001 tgtggttagc gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct gctggcagct
    27061 ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc aggctttcca
    27121 gcgccgcgtt ggcgttgttt acgtaggtct gatggtgttt ggtgtggtgg atttccatgg
    27181 tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat ggcagggtat
    27241 agctcatatt catctccagt attgtcgggc ggccgattgt taatgccgcg taagcagttg
    27301 gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcgtaag
    27361 ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt aatgccgtga
    27421 ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc acgattttta
    27481 cactcatctc gtcggagatg tgaagcgacg aaaaatgatg aggataagaa gatgagtaac
    27541 gcgattacga tggggatatt ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac
    27601 gctccgttca aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt cggtgggatt
    27661 gtttcgtgga ttattctgcc gtgggccatc agcgccctgt tactaccgaa tttctggacg
    27721 tattacagct cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg
    27781 gggatcggta atatcaacta tggcctgacc atgcgttatc tcggcatgtc gatgggaatt
    27841 ggcatcgcca ttggcattac gttgattgtc ggtacgctga tgacgccaat tatcaacggc
    27901 aatttcgatg tgctgattaa caccgaaggc ggacgcatga cgttgctcgg cgttctggtg
    27961 gcgctgattg gcgtagggat tgtaactcga gccgggcagt tgaaagagcg caagatgggc
    28021 attaaagccg aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt
    28081 ttctctgccg ggatgtcctt tgcgataaac gccgcaaaac cgatgcatga agccgctgcc
    28141 gcactaggcg tcgatccact gtatgtcgct ctgccaagct atgtcatcat catgggcggc
    28201 ggcgcgatca tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa ggatttgtcg
    28261 ctaaaagccg acttctcgct ggcaaaaccg ctgatcactc acaatgtgtt actctcagca
    28321 ctgggcgggt tgatgtggta tctgcaattc tttttctatg cctggggcca cgcccgcatt
    28381 ccggcgcaat atgactacat cagttggatg ctgcatatga gcttctatgt attgtgcggc
    28441 ggtatcgtcg ggctggtgct gaaagagtgg aacaatgcag gacgccgtcc ggtaacggtg
    28501 ttgagcctcg gttgtgtggt gattattgtc gccgctaaca tcgtcggcat cggcatggcg
    28561 aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc ggtttcccgg
    28621 gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg
    28681 attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac ctgtcgcaga
    28741 tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg
    28801 cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc
    28861 cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg
    28921 taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta
    28981 gcaaacggca catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc
    29041 gccatcccca cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga
    29101 atcgccccct gccagtcaag attcagcttc agacgctccg ggcaataaat aatattctgc
    29161 aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat gtaaaagaga
    29221 tcgccacggg taatgcgata agggcgatcg ttgagtacat gcaggccatt accgcgccag
    29281 acaatcacca gctcacaaaa atcatgtgta tgttcagcaa agacatcttg cggataacgg
    29341 tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac
    29401 tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt
    29461 acaaatacgt tgagaagatt cgcgttattg cagaaaacca tcccgtccct gacgaatatc
    29521 acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa
    29581 tccacagcga taggcgatgt cagtaacgct ggcctcactg tggcgtagca gatgtcgggc
    29641 tttcatcagt cgcaggcggt tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag
    29701 ctgccgatgt agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac
    29761 ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt
    29821 ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat
    29881 ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac
    29941 cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc
    30001 atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga
    30061 gcgatacagc acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg
    30121 atcgcgtacg aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg
    30181 aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa
    30241 atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc
    30301 cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat
    30361 cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt
    30421 ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaattcagc
    30481 aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca
    30541 gtaacgagaa ggtcacgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg
    30601 atcacattat gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg
    30661 tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt
    30721 ttaaaaatgg gctgcatagc cagaacggtt atgtcacctg gaatgtggat agcctggaaa
    30781 gtgccattcg ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg
    30841 ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc
    30901 tgcccgttgc ttatcgcgat agccgctcca atggcctaat ggcgcaggca cagcaacaac
    30961 tcggcaaacg cgatatttat caacgtagcg gcatccagtt tctgcccttc aatacgcttt
    31021 atcaactgcg tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc
    31081 tgctgatgcc ggattacttc agctatcgcc tgaccggcaa gatgaactgg gagtacacca
    31141 atgccaccac cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg
    31201 cgtggagcgg ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg
    31261 ggcactggat ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata
    31321 ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg cgccgcttat ctctcttctg
    31381 gcacctggtc attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg
    31441 cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta
    31501 tgggcttatg gctgcttcag cgagtgcttc aggaacggca aatcaacgat ctcccggcgc
    31561 ttatcgccgc gacacaggca cttccggcct gtcgcttcat catcaatccc aatgacgatc
    31621 gctttattaa tcctgaagcg atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc
    31681 aaccgatccc ggaaagtgat gctgaactgg cgcgctgtat tttcgacagt ctggcgctgc
    31741 tgtatgccga tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc tcgcaactgc
    31801 atattgtcgg cggcggctgc cagaacacgc tgctcaacca gctatgcgcc gatgcctgcg
    31861 gtattcgggt gatcgccggg cctgttgaag cctcaacgct cggcaatatc ggcatccagt
    31921 taatgacgct ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg
    31981 cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcccactat gtggcgcaga
    32041 ttcactctac acgacagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg
    32101 ggagctggcg aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca
    32161 acttgatcgt ttacccgttt caatgcactg ctggcagggc gatgatgttt ccggttttga
    32221 aaacccggaa ggttcgctga ccggtgggat tcaagctact ggtaattatc cgggcaaagc
    32281 gcgtaatgcc agtgagctac gtaccgatct ggaacaggct atgcggctga ttccggggcc
    32341 gaaacggctt aatttacatg ccatctatct ggaatcagat acgccagtct cgcgcgatca
    32401 gatcaaacca gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct
    32461 ggattttaac ccctcctgct tttcgcatcc gctaagcgcc gatggcttta cgctttccca
    32521 tgccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc gtcgcgtttc
    32581 ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga tcccggatgg
    32641 tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg cagcactgga
    32701 tgaggtgatc agcgagaagc taaaccctgc gcaccatatc gacgccgttg agagcaaatt
    32761 gtttggcatt ggcgcagaga gctacacggt tggctccaat gagttttaca tggggtatgc
    32821 caccagccgc cagactgcgc tgtgcctgga cgccgggcac ttccacccga ctgaagtgat
    32881 ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagccg
    32941 tccggttcgc tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcaattgc
    33001 cagtgagatt gtgcgtcacg atctgtttga ccgggtgcat atcggccttg acttcttcga
    33061 tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga aaaaagccct
    33121 gctgcgtgcg ttgctggaac ctaccgccga gctgcgcaag ttggaagcgg cgggcgatta
    33181 cactgcgcgt ctggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtctggga
    33241 aatgtattgc caacgtcacg ataccccaac gggtagcgaa tggctggaga gcgtgcgggc
    33301 ttatgagaaa gcgattttga gccagcgtgg gtaaacactg ccggatgcgg cgcgagcgcc
    33361 ttatccggcc tacaggtcgg caacagttgt aggcctgata agacgcgaca gcgtcgcatc
    33421 aggcattgat tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gtgcatctga
    33481 caacaccgaa tttacaggaa cacagaacat gcaaaacatt acccagtcct ggtttgtcca
    33541 gggaatgatc aaagccacca ccgatgcctg gctgaaaggc tgggacgagc gcaacggcgg
    33601 caacctgacg ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca acttccaccc
    33661 acaaccgcgc tatattccgc tcagccagcc catgccttta ctggcaaata cgccgtttat
    33721 tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt gatcctgcag ctaacttagg
    33781 cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt ctctgggggt taaccaacga
    33841 agccgttccc acttccgaac ttccggctca cttcctttcc cactgcgagc gcattaaagc
    33901 caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac
    33961 ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag gcagcaccga
    34021 gtgtctggta gtgttcccgg atggcgttgg cattttgccg tggatggtgc ccggcacgga
    34081 cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt tgtggtcctt
    34141 ccacggcgtc ttcggcagcg ggccgacgct ggatgaaaca ttcggtttaa tcgacaccgc
    34201 agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga aacagaccat
    34261 cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac tagccagtgc
    34321 gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg ttaaccccga
    34381 cgctcacgaa gagtatcagc gtcggcataa ccccatctgg ccggaactgg aagcagtgtt
    34441 gaaatctcac ggtgcgcata actacgccat ttacctcgac aaagcgcgta atctgctgtt
    34501 tgccacggta gagattgaat ctgaagagcg ctggaatgcg gttgccagta ctgatgtttg
    34561 ccaacgttgg tggaaatata tgaccgacgt catgcccgcg aacccggata acagcccggt
    34621 gagtagcgaa ctgcaagaag tgttttacct gccgtaattg cctaatgtgc cggagacgcc
    34681 tccggcacaa tatttttaat ttcgatttga aaaataaaca tattacacct tcagttaata
    34741 tatcccggta acttatattt ccccgatgca ttgtgagtaa atcacaaaaa taatgaataa
    34801 ctcattaatg attcatgtgg tttatttaaa taaccattat gtgcattact ccgcaaatct
    34861 gacctttcac tctgtctcaa attgtcattg tttaccgaca tatcgcctct catcacttaa
    34921 tcgctccacc attacaattg attaaaatat atttcatgga gtccgattaa tggcagctct
    34981 tactgcaagc tgtattgacc tgaatattca gggcaatggc gcttattcca ttctgaagca
    35041 gttggcgaca atggcgttac aaaacggttt tatcaccgac tcacaccagt tcctgcaaac
    35101 cctgctgctg cgcgaaaaaa tgcactctac tggatttggt tccggcatcg ccgtgccgca
    35161 cggtaaaagc gcctgcgtta aacaaccgtt cgtgttattc gcccgcaaag cgcaggctat
    35221 tgactggaaa gccagcgatg gcgaagacgt caattgctgg atctgcctcg gcgtgccgca
    35281 aagcggcgaa gaggatcagg tcaaaatcat cggtacactg tgtcgcaaaa ttatccacca
    35341 ggattttatt caccaactgc gacgaggcga cgccgaccag gtgtttgcct tgttaaatca
    35401 aaccctcagc tcataaggaa gtggcgatgg agtcatcctt acgtattgtc gcgatcacca
    35461 actgccccgc cgggatcgct cacacctaca tggtggcgga agccctggaa cagaaagccc
    35521 gttctctcgg ccataccata aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct
    35581 tatccagcga agagattgcc gctgccgatt acgtcattct cgctaccggg cgtggcctga
    35641 gcggcgatga ccgggcgcgg tttgccggga agaaggttta tgagattgcc atctcccagg
    35701 cgttgaaaaa tatcgaccag attttcagtg aattaccaac aaactcgcag ctttttgccg
    35761 cagatagcgg cgtgaagctc ggcaaacagg aagtacaaag cggcagcgta atgagccacc
    35821 tgatggcggg cgtctctgcg gcgctgccgt ttgttatcgg tggcggtatc ctggtggcgc
    35881 ttgccaacat gctggtgcag ttcggcctgc cgtataccga tatgtcgaaa ggcgcgccgt
    35941 cgtttacctg ggtggttgaa tccatcggtt atctcggctt cacctttatg atccccatca
    36001 tgggcgctta tattgcctcg tcgattgccg ataaacccgc tttcgctccg gcgtttctgg
    36061 tttgctatct ggcgaatgac aaagccctgc tcggcaccca gtcaggtgcg ggattcctcg
    36121 gcgcggtggt gctggggctg gcgattggct atttcgtctt ctggttccgt aaagtgcgcc
    36181 ttggcaaagc attacaaccg ctgctcggtt caatgctgat cccgttcgtt actctgctgg
    36241 ttttcggcgt gctcacttac tacgttatcg gaccggtaat gtccgacctc atgggcgggt
    36301 tgctgcactt cctgaacacc attcctccat caatgaaatt tgcggcagcg tttctggtcg
    36361 gtgcgatgct ggcgttcgat atgggcggcc ccatcaataa aaccgcctgg ttcttctgct
    36421 tctcactgct ggaaaaacac atttatgact ggtacgccat cgtcggcgtt gtcgcgctga
    36481 tgccgcccgt cgcggcgggt cttgcgacgt tcattgcgcc caaactgttt acccgacagg
    36541 aaaaagaggc tgccagtagt gccattgtgg tcggtgccac agtcgcaacc gaacctgcta
    36601 ttccttacgc tctggcagct ccgttaccga tgattaccgc caatacgctg gcgggcggta
    36661 ttaccggcgt gctggtgatc gctttcggaa tcaaacgtct ggcacctggt ttgggtatct
    36721 ttgacccgct gatcggcctg atgtcgccgg taggttcgtt ttatctggtg ctggcgattg
    36781 gcctcgcgct aaacatttca ttcattatcg tcctgaaagg attgtggtta cgccgcaaag
    36841 cgaaagccgc gcaacaggag cttgtccatg aacattgagt tactgcaaca gttgtgcgaa
    36901 gccagcgccg tcagcggcga tgaacaggaa gttcgcgaca tcctgataaa cacgctggaa
    36961 ccttgcgtta atgaaatcac ctttgatggt ctgggcagct ttgttgcccg taaggggaat
    37021 aaaggtccaa aagttgccgt tgtcgggcat atggatgaag tcggctttat ggtcacccac
    37081 atcgacgaga gcggttttct gcgcttcacc accattggcg gctggtggaa tcagtcgatg
    37141 ctcaaccacc gggtaaccat acgcacacac aagggattta aaatccctgg tgtgattggt
    37201 tccgtcgcgc ctcatgcgtt aacggaaaag caaaagcaac aaccgctgtc atttgatgag
    37261 atgttcattg atattggcgc gaacagtcgc gaagaagcgg aaaagcgcgg cgttgaaatt
    37321 ggcgatttta ttagcccgga agccaatttt gcctgctggg gcgaagataa agtagtcgga
    37381 aaggcgctgg ataaccgcat cggctgcgcg atgatggcgg agctactaca gacagtaaat
    37441 aacccagaaa ttacgctgta cggcgttggc agtgtggaag aagaagttgg gctacgcggg
    37501 gcacaaacct cggcggaaca cattaaaccg gatgtggtga ttgtgctgga taccgctgtc
    37561 gcaggtgatg ttccgggcat tgataacatt aaatacccgc tgaaactggg ccaggggccg
    37621 gggctgatgc tgtttgacaa gcgctacttc cccaaccaga aactggtagc agtgttaaaa
    37681 aactgtgccg cacataacga tttaccgctg caattttcca ccatgaaaac cggagcgacg
    37741 gatggcgggc gctacaacgt gatgggcggc gggcgtccgg ttgtcgcgct gtgtctgcca
    37801 acccgttatc tgcacgctaa cagcggcatg atttcaaaag ccgattacga tgctctgctc
    37861 acgctgatac gggattttct gacgacctta actgcggaga aagtcaacgc gtttagccag
    37921 ttccgtcagg tggattaatg ctaaacgaac gccagttaaa gattgtcgat ctgctggagc
    37981 aacagccgcg cacgcctgga gagctggcgc aacagactgg cgtttcaggc aggaccatcc
    38041 tgcgtgatat tgactatctc aacttcaccc ttaacggcaa agcccgcatt ttcgccagtg
    38101 gtagtgcggg ctatcagctg gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa
    38161 agcacgataa cgacgatcgg ctgctggcgc tgttattact gaatactttc actccccgtg
    38221 cgcaactcgc ctcggcgctt aatttgccag aaacgtgggt agcagagcgt ctgccccggt
    38281 taaaacagcg ttatgaacgc acttgttgcc tggccagccg ccctggtttg ggccatttca
    38341 ttgatgagac agaagagaaa cgcgttatct tgctggcgaa cttgctgcgc aaagatccgt
    38401 ttttaattcc gctggcgggc ataacacgag acaaccttca gcatttatcc acggcctgcg
    38461 acaaccaaca ccgctggccg ctcatgcagg gtgattatct ctccagcctg attctggcga
    38521 tttacgccct gcgtaatcaa ctgaccgatg agtggccgca atatcccggt gacgagataa
    38581 aacaaatcgt tgaacatagc ggtctgtttc ttggttataa cgctgtaaga accctgacgg
    38641 gtttgataga gaaacagcat cagcaagcgc aggtaatttc agccgatcat gtgctggggt
    38701 tgctgcaaag ggtgccgggc atcgcgtcat tgaatattat tgatacgcag ctggttgaga
    38761 acattaccgg gcatttatta cgttgcctcg ccgcaccagt gtggattgct gagcaccgcc
    38821 aaagcagcat gaataacctg aaagccgcct ggcctgcggc ctttgatatg agtctgcact
    38881 ttattacgct actgcgtgaa cagctcgata ttcccctttt cgacagcgat ctgatcggtt
    38941 tgtattttgc ctgtgcgctg gagcggcatc aaaacgaacg ccagccgatc attttgctct
    39001 cggaccagaa cgcgattgcc actattaatc agctcgccat tgagcgcgat gttttaaatt
    39061 gtcgggtaat tattgcccgt agcttaagcg aacttgttgc cattcacgaa gagattgagc
    39121 cgttattgat cattaacaat agccattatt tactggaaga cgcggtaaat aattacatca
    39181 ccgtaaaaaa tatcattacg gctgccggta tcgaacaaat aaaacatttt ctggcgacgg
    39241 catttattcg ccagcaaccg gagcgttttt tctctgcccc cggaagtttt cattattcga
    39301 atgtacgcgg tgaaagctgg caacatatta cccggcaaat ttgtgcgcaa ttagtggcac
    39361 aacaccatat taccgccgat gaagcacaac gcatcatcgc ccgcgaaggc gaaggtgaaa
    39421 acctgattgt taatcgcctc gccatcccac attgctggag cgaacaggag cgacgttttc
    39481 gtggattttt tattaccctc gcccacccga tcgaggtgaa taacgaaatc attaaccatg
    39541 tgttgattgc ctgcgccgcc gccgatgcgc gtcatgagct aaaaatattt agctatctgg
    39601 caagcgtatt gtgtcagcat ccggcagagg ttattgccgg gttaacagga tatgaggcat
    39661 ttatggagtt acttcacaag gggtgaactg tgtcggatgc gccgcatccg acactgcatt
    39721 tatcatcaaa aacgtttaac gcaggctatt atagtcatca accatttcgt tgtactcgcg
    39781 taatgctctc gggaaagagt cgtcgaccat ccagcttgag tttgcgtcct gcagttgctc
    39841 tttatcccag tcagagtacg ggactttatc tctgacgcgg cgaacgtatt ttttagcctc
    39901 gagttgatac tggcctgccg aattaataaa cgagaaattc atgccgcctt tatccgcttc
    39961 tttggcctgc gccaccagcg tttccagttc agacactttc ttcattgccg cttctgcatc
    40021 aaacttatct tgcgatatca ggttattaat ttgtttcgct gaaatcatga ctgccagaga
    40081 gtaatagtgg aatgttttcc cttctctttc ttcaatattt ttcagttccg caagctggcg
    40141 cttatcgtta atttcctgaa tcgccgcatg ataagattct gctacagctt caaaggcttc
    40201 cagatttttt aagaatgtct ggtgcagcgt tttccctttc gcaaacgcat cgtctttata
    40261 attttcctgg gtgtaatatt tatccatttc gttaatggta ttacccaacg ccacggcagc
    40321 atcgatataa ctcaccgcca cgccatcaat aggttgcagc gcaggcgtta acgccacagc
    40381 actttttacg cctttttcac actcagcgag gttggattca ctaatgccgt agatgccata
    40441 aacagtacgc tcttcaccgg taggcccctg tttaaaatct ttcagccagt cagcataacg
    40501 cgccagactg ctctgtacag ggatctgtaa cttgttatag cacttgatat agacatccat
    40561 tttttcgtcg aaaaccgctt gttcatcgac caccggttgc gcaggtgttt caggtttagc
    40621 ttctgctttt gcaacgggag cttcaggagc tggtgcggca ggttgactat tggcaggcgg
    40681 gggcgtttct gtttcggctt ttttatcatc acaacccgtc agaccgaggg ccatgatggc
    40741 gactataata gcggaagata ataaattcct tttcatttaa ttgccttata aatatataga
    40801 taagagagtg aacataataa atgaaaaaag aaaacgccac tacacgcgtt ttatttataa
    40861 ataaaataat aaaattcacg aaatgaataa gtcctgcaat ttaatatata tcgcaggact
    40921 tattttaatt aattgctcaa acgctgcgga tgggtataaa ccgttgcccg acccggctta
    40981 caaaaaccca ccagcgtcag attacaacgc tcagccactt ctacagcaag cgtggtcgcg
    41041 gcagacaccg caaacaaaat ctctacgccg cacatcgccg acttttgcac catttcataa
    41101 ctggcacgac tggaaaccag taccgcacct tgctgccagc tttccccttc ttgtgaccgg
    41161 cggcctaaca gtttgtccag cgccacatgg cgacccacgt cttcatgccc gccgaccagt
    41221 tcgccagatg gcaacatcca ggcagcggcg tgagtacaac cagtcagttg ccccactggc
    41281 tgaaaatcgt tgagatgacg taatgcatca tccagtttgt tgagatcaaa cgtctgggtg
    41341 aacggtagcg gctgcaccgg ttttccgatg tcattaagtt gctccacgcc gcatacgcca
    41401 cagcccgtac gtcccgccag cgcccggcgg cgctccttca accccataaa gcggcggctg
    41461 gaaagctcaa tttgtacttc aagaccatta caggaaggaa cgacatccat gccgaagata
    41521 tcgcgcggac tttctattat cccctcggaa agcgaaaaac cgagcgcaaa gtactcaagg
    41581 tctttgggcg acgccatcat caccacatgc gaaatgccgt tgtagaccag cgcaacggga
    41641 acttcttccg cgacctcatc taaccgtggg tgttgtaaat catcacgccg ccataactca
    41701 atttgacgaa cacctgtgat atttgtcaca ttttcgattt ctttttgctg tgtttttttc
    41761 actttctctt aaccagataa gaacacacac accaacatta tggtattctg ttacaaaccc
    41821 ttcctggatg gagggaaatt gagccaattc tggacctttg cggccccttc cgcaaagaaa
    41881 aataactccc actccctgca cacgcagcaa gcgaatgtaa atgggacgtg acaatgtcga
    41941 aacaaggagc aatccatgca ggtcagcaga aggcagttct ttaagatctg cgctggcggt
    42001 atggcaggca ccacggcagc ggcactgggt tttgcaccca gcgtagcgct cgcggaaacc
    42061 cggcagtata aactgctgcg cacccgcgaa acccgtaata cctgcaccta ttgttccgta
    42121 ggctgtgggc tgttgatgta cagcctcggt gacggagcaa aaaacgccaa agcatctatc
    42181 ttccatatcg aaggcgatcc ggatcacccg gtcaaccgcg gtgcactttg tccgaaaggc
    42241 gctggcctgg tggatttcat ccactccgaa agccgtctga agtttccgga ataccgtgcg
    42301 ccaggttctg ataaatggca gcaaatcagt tgggaagagg cgtttgatcg catcgccaaa
    42361 ctgatgaaag aagaccgcga tgctaactac attgcgcaaa acgccgaagg cgtgactgtt
    42421 aaccgctggc tctccaccgg gatgctgtgt gcttccgcgt cgagtaacga aaccggctat
    42481 ttaacgcaaa aattctcccg cgcgctgggt atgctcgcgg tcgacaacca ggcgcgtgtc
    42541 tgacacggac caacggtagc aagtcttgct ccaacatttg gtcgcggtgc gatgaccaac
    42601 cactgggtcg acatcaagaa cgccaacctc gtcgtggtga tgggcggtaa cgccgctgaa
    42661 gctcacccgg tcgggttccg ctgggcgatg gaagccaaaa ttcacaacgg cgcgaagctg
    42721 attgtgatcg atcctcgctt tacgcgtacg gcggcggtag ctgactacta tgcccctatt
    42781 cgttccggta ctgacattgc tttcctgtca ggcgtattgc tgtacctgct gaacaatgaa
    42841 aaattcaacc gcgaatacac cgaagcctat accaacgcca gcctgatagt gcgtgaggat
    42901 tacggctttg aagatggcct gttcaccggc tacgacgcgg aaaaacgcaa gtacgataaa
    42961 tcctcctgga cttatgaact ggacgaaaac ggcttcgcca aacgcgatac cacgctgcaa
    43021 catccgcgct gcgtgtggaa cttgctgaaa cagcacgttt cccgctacac gccagatgtg
    43081 gttgaaaaca tctgtggtac gccaaaagac gcgttcctga aagtctgcga atacatcgca
    43141 gaaaccagtg ctcacgataa aactgcctcg ttcctgtatg ccctcggctg gacgcaacac
    43201 tccgttggtg cgcaaaacat tcgtacgatg gcgatgatcc agctgctgct cggcaatatg
    43261 gggatggccg gcggcggcgt taacgccctg cgcggtcact ccaatattca ggggctgacg
    43321 gacctggggc tactgtcgca gagtctgcca ggttacatga cgctgccaag cgagaagcag
    43381 accgatctgc aaacctacct taccgccaac acgccaaaac cgctgctgga aggccaggta
    43441 aactactggg gcaactaccc gaaattcttc gtctctatga tgaaggcctt ctttggtgat
    43501 aaagcgacgg cggaaaatag ctggggcttt gactggttgc cgaagtggga taaaggctac
    43561 gacgtcctgc agtacttcga gatgatgaaa gagggcaagg tcaatggcta tatctgccag
    43621 ggctttaacc ctgttgcctc attcccgaac aaaaacaaag tgatcggttg tctgtcgaaa
    43681 ctgaagttcc tcgtcaccat cgacccactg aacactgaaa cctctaactt ctggcagaac
    43741 cacggtgagc tgaacgaagt tgactcgtcg aagatccaga ccgaagtgtt ccgtctgcca
    43801 tcgacctgct tcgcagaaga gaacggttca atcgttaact caggtcgctg gttgcagtgg
    43861 cactggaaag gtgcggacgc cccggggatt gcgctgactg atggcgagat cctctccggt
    43921 atcttcctgc gcttgcgcaa gatgtatgcc gaacagggtg gcgcgaaccc ggaccaggtg
    43981 ctgaacatga cctggaacta cgccattccg catgagccga agtcggaaga agtagcgatg
    44041 gagagcaacg gtaaggcgct ggccgatatt accgatccgg caaccggtgc ggttatcgtc
    44101 aagaaaggcc aacaacttag ctcgttcgcc caattgcgcg atgacggtac aacctcctgt
    44161 ggctgctgga ttttcgccgg tagctggacg ccggaaggca accagatggc gcgtcgtgat
    44221 aacgccgatc cgtctggcct cggtaacacg ctgggctggg catgggcatg gccgcttaac
    44281 cgccgcattc tgtataaccg cgcctccgca gatccgcagg gtaacccgtg ggatccgaag
    44341 cgtcagttgc tgaaatggga cggcactaag tggaccggct gggatattcc ggactacagc
    44401 gcagcgcctc cgggtagcgg cgtcgggccg tttatcatgc agcaggaagg catggggcgt
    44461 ctgtttgccc tcgataagat ggcggaaggt ccgttcccgg aacactacga gccgtttgaa
    44521 acgccgctgg gaactaaccc gctgcatcca aacgttatct cgaacccggc tgcacgtatc
    44581 tttaaagacg acgccgaagc attgggtaaa gccgataagt tcccgtatgt cggaaccacc
    44641 tatcgtctga ccgagcactt ccactactgg accaaacacg cgctgttgaa cgcgattttg
    44701 caaccagagc agtttgtgga aatcggggag tcgctggcga ataaacttgg cattgcccag
    44761 ggcgataccg tgaaagtctc ctccaaccgt ggctatatca aagccaaagc ggtggtgacc
    44821 aaacgtattc gtacgctgaa agcgaacggc aaagatatcg ataccatcgg tattccgatt
    44881 cactggggtt atgaaggcgt agctaaaaaa ggctttattg ccaatacgtt aacgccattc
    44941 gttggtgatg cgaacacgca gacgccggag tttaagtcct tccttgtgaa tgtggaaaag
    45001 gtgtaacgga gacgacatat ggcttatcaa tctcaagata tcattcgtcg ttccgcgact
    45061 aacggtctga cccccgcgcc tcaggcgcgg gacttccagg aagaagtggc gaaactcatc
    45121 gacgtcacca cctgtatcgg ctgtaaagcc tgtcaggtgg cgtgttcaga gtggaacgac
    45181 attcgcgata ccgtcggcaa taacattggg gtgtacgaca accccaatga tttaagcgcc
    45241 aaatcgtgga cggtaatgcg cttctcggaa gtggagcaga acgacaaact ggaatggctg
    45301 atccgcaaag acggctgtat gcactgttcc gatccaggct gcctgaaagc gtgcccggcg
    45361 gaaggtgcaa ttattcagta tgccaacggt atcgtcgact tccagtccga gcagtgcatt
    45421 ggctgcggtt attgcattgc gggctgtccg ttcgacattc cgcgcctcaa cccggaagac
    45481 aaccgcgtct acaaatgtac gctgtgcgtt gaccgcgtgg tggttgggca agaaccagcc
    45541 tgcgtgaaga cctgcccaac gggcgcgatt cacttcggta cgaaagagtc gatgaaaacg
    45601 ctggcgagcg agcgcgttgc tgagctgaaa acccgcggtt acgacaatgc gggtctgtac
    45661 gatccggcag gcgtcggtgg tacacacgtc atgtacgtgc tgcaccatgc tgacaagcca
    45721 aatctgtatc atggcttgcc ggagaacccg gaaatcagcg aaaccgtgaa attctggaaa
    45781 ggcatctgga aaccgctcgc ggctgttggc tttgcggcta cctttgcggc cagtatcttc
    45841 cactacgtgg gtgtcggtcc gaaccgtgcg gatgaggaag agaataatct gcacgaagag
    45901 aaagacgagg agcgcaaatg aaacgacgtg acaccatcgt gcgctacacc gcgccggaac
    45961 gtatcaacca ctggatcacc gccttctgct tcatcctggc ggcggtgagc gggctgggct
    46021 ttttgttccc gtccttcaac tggttgatgc aaatcatggg cacaccgcag ctggcgcgaa
    46081 ttctgcaccc gtttgtcggc gtggttatgt ttgcctcgtt catcatcatg tttttccgtt
    46141 actggcatca caacctaatc aatcgggatg atatcttttg ggcgaagaat attcgtaaga
    46201 tcgtcgtcaa cgaggaagta ggtgacaccg ggcgttataa cttcggtcag aaatgcgttt
    46261 tctgggcggc gattattttc ctggttctgc tgctggtgag cggcgtgatt atctggcgtc
    46321 cttattttgc gcctgctttc tcaatcccgg tgatccgatt cgcgttaatg ctgcattcat
    46381 ttgccgcagt ggcgttaatt gtggttatca tggtgcatat ctacgccgcc ctttgggtga
    46441 aaggcacgat taccgccatg gtggaaggat gggttaccag cgcctgggcg aagaaacatc
    46501 acccgcgctg gtaccgtgag gtccgcaaga caacggaaaa gaaagctgaa tgagtattcg
    46561 cataatcccg caagatgagc tgggttcgag cgagaaacgt acggcggata tgattccgcc
    46621 gttattgttc cctcggctca agaatttata caaccgccgc gccgagcgtc tgcgcgagct
    46681 ggcagaaaat aatccgctgg gtgattacct gcgctttgct gcgcttatcg cccacgccca
    46741 ggaagtggtg ctgtacgacc atccgctgga gatggatctg accgcgcgca ttaaagaagc
    46801 cagcgcacag ggcaagcccc cgctggatat tcacgttctg ccgcgtgata agcactggca
    46861 aaagctgctg atggcgctga ttgctgagct gaaacctgaa atgagcggcc cggcgctggc
    46921 agtgattgag aatctggaga aggcatcaac tcaggagctg gaagatatgg ccagcgcact
    46981 gtttgcctct gatttctcat ccgtcagcag cgataaagcg ccgtttatct gggctgcact
    47041 gtcgctctac tgggcgcaga tggccaatct gatccccggc aaagcccgcg ctgaatacgg
    47101 cgagcaacgt caatattgcc cggtttgtgg ttctatgccg gtgtccagca tggtgcaaat
    47161 tggcaccact cagggtctgc gttacctgca ctgcaacctg tgtgaaaccg aatggcacgt
    47221 agtgcgcgta aaatgcagca actgcgagca gagcggcaaa ctgcattact ggtcgctgga
    47281 tgacgaacag gccgcgatta aagccgaaag ctgcgatgac tgtggcactt acctgaagat
    47341 tctttatcag gaaaaagaac cgaaagttga agccgtggca gatgacctcg cttctctggt
    47401 actggacgcg cgaatggagc aagaaggcta tgcccgcagt tccatcaacc cgttcctgtt
    47461 tccgggtgaa ggggagtaat ctctgacgtt ttccgggtgg cgctggtcgc ccggattaca
    47521 gctcataggg tgtgacgaca ccatctctcg tattccgcgt tacccattca agatcatgcc
    47581 gcaattgtct gtttgcctcg ttcttccctc caacacatcg ataaggttat gggtatgtca
    47641 ctgatgacaa gcgtatgatt gcctgatgcg ctgcgcttat cagccctacc aataaacttc
    47701 aatatattga atttgcatga ctttgtaggc cggataaggc gttcacaccg catccggcat
    47761 gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt accattcctc
    47821 gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga tgccaggaac
    47881 actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt cccttaatag
    47941 gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa tcacctcatt
    48001 cgataaatcg agtaaaattc tgcccatatc tatacctacc agagagttca taaatatctc
    48061 gccaatatca tttttgaatc tatggagaga aaagtaccct tgtcgaatct ttaaagaaag
    48121 ctcatttacg aatcattttt tatttttgcg aggcgttttc cagatatgat gtaacagttg
    48181 cagttattat aagttcaagt aaaacaacct tgtattaata ccagaataag ggcatctggt
    48241 tttttatatt caagaataaa aaaatttttg tcattcctta tgatccttac actgatcata
    48301 aacacctact gtctcacccc tttgcctgcc tctctttgct ttacgcaccg gaaattgctg
    48361 ccgctccaga tattgctcaa ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca
    48421 gccccgaagc ttgttcagcc gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt
    48481 catcgccatt accaccacct tacatatatt actcattaat gtatgtgcga aaattacgcc
    48541 ggaaaatcat ttaaaaaaag cggatttttg catgttgttt tgtttttgcg aggtgttttc
    48601 cagaaataat aagagtgttg cagttgtttc atttattact caaaaatggt ttagttatcg
    48661 cccaggatga gttatggatg ctatgaatat attatattca gggatgttta ttttttgatt
    48721 ggatctttat ttgcataatc tgaatggtgt acccgaaaaa tgtcagcatc ctaacagcac
    48781 ccggatttaa ggtgaaatta atcatccatc acagaccggt cagtactgcc accaaccggt
    48841 ctttacttgc tcatcgttat aattttgttg gtaacagttc gggaaacaag gaattccttc
    48901 cggaaccccc cggtatcatc cgatagcatt gacgtcaatc atcagcgcga cgatgagttt
    48961 ttttaatcgc atcatagggt ggcgacgttc agcacgatta cgggtcatag caaaactcct
    49021 gggcaggcga ccactatgcc gctttcacag gagttctgaa tgctagtgct ttctgtatgg
    49081 cataaccatg cctctaacgg ttaagtgctt gcagcacatg ttgcggatca ttggcaatcg
    49141 ctcgcagcaa agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc cagttacgca
    49201 acgtgtctac gcttacagaa ataagatcgg caaatttaga ttgtgacaac cctgaagcgc
    49261 ggcgtatttc tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc tgtcgcgttc
    49321 cttcaataat ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat
    49381 ccatttacca cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat
    49441 ctttaatacc cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa
    49501 aataaataac cctgacacca gcacgtttcc cctttcctcc tgaaacccat cgaacctttc
    49561 ttaacccacc agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact
    49621 gaaaacgact gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg
    49681 tttcgatgaa gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc
    49741 attgacgtac ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg
    49801 ccgggtttgt ctttagattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc
    49861 tgtatccatc cagcaaaaac gggtgtataa gactgtacat tcatacagca aaataagttc
    49921 tactatggca ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg ctgcgggtcg
    49981 cccctcatcg cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt
    50041 ccttgcgggt gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgac
    50101 cttacgaata gtgtcgccgc tgaatgcccc gacattactc ccgacgataa tttattacta
    50161 cggtggctgt tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc
    50221 ctattacggg caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg aacatacttt
    50281 ccccgccgcc catgatgatg cgcaacgagg tgcagaaata gtctggcaac acgccgaacg
    50341 actcggcgtt gataaacaac gtataactct tgccggtgac agtgcgggcg gacatctggc
    50401 gctggtaact gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt
    50461 aatttatcct atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga
    50521 ttacatcatc acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga
    50581 acgcaggcat cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt
    50641 acatatcatt accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg
    50701 catgacaggg caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt
    50761 ttttcagtta ggtggtatca gcgaggccgc gcgatcggca ctacgggatg ttgcctggag
    50821 agcgggaagg taacttcgga cctgggccct gaaaaggttt tcagattgaa aaccttttca
    50881 gtcaagtaaa acgcccctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct
    50941 tcgcgggcag aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga
    51001 tttcaacttg catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc
    51061 ttaaggcacc aaggtcggct accgcatgag gtttaccgct aatcggcttg ctgtagcgga
    51121 tatgcgcatc cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga
    51181 taagccccca accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt
    51241 gcggattctg attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt
    51301 gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct
    51361 gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca
    51421 aaaaatggcg aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca
    51481 gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt
    51541 cctgacgcgc caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca
    51601 cgtccgggtg aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacagtc
    51661 ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat
    51721 cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc
    51781 gaaactgata gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag
    51841 cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa
    51901 tgtcacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg
    51961 cgtgttcatc tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg
    52021 atccggtgat gcgcctttgg agaaacttgg gcgcatccct cgttcggtat ctgcggcgag
    52081 ggtaaactgg gaaaccacca gtacactgcc gcccgcctgt tgcacgttga gattcatctt
    52141 gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt
    52201 ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc
    52261 cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat
    52321 ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg
    52381 acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga
    52441 atggggatca ccgccagcac accgtaaatg agctgatatg acgggaacat ggtgatataa
    52501 agcgcgaaac ctttctttcc tgcttcgaac aggagcgcgg cgacaaacgc gccgacaatc
    52561 gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc
    52621 cacgacaaca gcagcggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc
    52681 gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc
    52741 cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg cgctcgttta
    52801 ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcagcaataa cgcggtgacg
    52861 atcagcccgc acgccccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata
    52921 taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgtaac
    52981 tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc
    53041 agcggcacta atgagagcaa cgacacatag gcaaggttac ctgccagggt tgtcatgttg
    53101 tcctcatcaa tgcgttgcca gagtagtttt agccaggccc atagtggacg ggtacggtgc
    53161 ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg
    53221 ttttatcttt caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg
    53281 cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct
    53341 ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag
    53401 cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat
    53461 tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt
    53521 ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg
    53581 gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct
    53641 catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac
    53701 gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac
    53761 ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc
    53821 gggaaaggat gggtttggct atgtggcaag gtatgaagcc aggttaacgg tcacaaaaat
    53881 ctacgcgtct tcctggggaa gaatgatttc aatttggctg tcttgtaagc gtgattttag
    53941 cgcagcgggc ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt taccaatttc
    54001 aaccgccgcc gaagcatgat acttagtgtg atcggcgacc agcagaatat ttctcgcgtg
    54061 cgccatcatc gttttcacca cgttagcttc gtttacatca aactccatca acgcgccatc
    54121 gctctcaatc gccccaacgc ttgttaccag ataatcagca cgaaaatcag ccacaaagga
    54181 cgccgctgaa gggccaatga tcccgctatt atgagagcgc aacgtaccgc cgggcaccat
    54241 cacttcaaag cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt tggtgattat
    54301 ccgcaaatga ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg taccaatggt
    54361 gataaatatt gttgaaccat caggaatata gtctgccacc gcttcggcaa tcgctttttt
    54421 ttcctcggtt tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc tggaagcccg
    54481 acccgcgcca ccgtgatggc gcgtaatcag gccttgctcg cttaatttac gaatatcccg
    54541 gcggaccgtc tgcgtggaga catccagcag atttgccagc tcatcaatat tcatataccc
    54601 acgctcggcg atcagcatga ggagttgatc gtgccgcggg ttaccggtta gttcggtaag
    54661 gctcatgaaa attcctcgaa aaaccatcac cctggcattt tatacaaaaa gtgacaaaaa
    54721 agatcgggtt tgatcacagt cagggatccc ggctcgtcca ccgggacgtg tgcattttaa
    54781 cgccgctaca ccgctggcga agcggactga ctccgctaaa tccccacttg ttgccagcgc
    54841 caccgccaaa gcgccgtgaa aaacatcacc cgcacctgtg gtatctacca catcaacttt
    54901 gaaggccggt tgatgctgac gcccaccatt ttccagccag tcgcagcctg cgctaccctg
    54961 ggtcacatag acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac tggccatctc
    55021 tttcacgccc gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat cgcttaatgc
    55081 caccagctca ctgatatcct gcggcgtaat gtccccgtcc agaacggtca tcacacccgc
    55141 ctgacgggcc agggtgaagg cttttttagc gccgtcgtgc cagcgtacat ctgccagcac
    55201 aacatcccac tgagagaaat caatttcctc caaccactct gcgtcaggca gcagatccgg
    55261 gctggggtag ttaatgatta tccgctcgcc tttggtatcc accatgatgg cggattgcga
    55321 agatttcgcc tggttatacc gtttggtgta acgggtgtta acgccccagg attccagctc
    55381 tgccagcagg ctgttgcccg tgtcgtcatc acctacgcga ccaataaaat cgacctgcgc
    55441 ccccagcctt gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa cttccgtata
    55501 atttctcgcc acgtatttac cgctctccgt cggtaaccct tccacgtaat agatgcgatc
    55561 catcacggtt atacctacac aagcaacacg aatcatggtc attccttaaa cattttggga
    55621 tgcgttcatt ttaatttcac caattgttta aaaagtgacg gctgtcaatt ttttgacgat
    55681 aaattacaaa tacgatcaaa aacagacaag caaagcatgt cataaatgac aaaaagtgac
    55741 atcactgtat tcaggagagg gttatggcag caatcgcgtt tatcggttta ggacaaatgg
    55801 gttcgccaat ggcgagcaat ttattgcagc aagggcatca acttcgcgtc tttgatgtga
    55861 acgccgaagc tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc aacccggcgc
    55921 aggccgctaa agatgccgaa tttatcatta ccatgttgcc gaatggcgat ctggtgcgca
    55981 acgtgttgtt cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg ctggtcattg
    56041 atatgtccac catccatccg ctgcaaaccg ataaattgat tgccgatatg caagccaaag
    56101 gcttcagcat gatggatgtt ccggtaggcc gtacttctgc aaatgccatt accggtactc
    56161 tgttactgct ggctggcggc accgctgaac aagttgaacg tgccacaccg atcctgatgg
    56221 cgatgggcag tgagttgatc aactctggcg gtccgggcat ggggatccgc gttaagctca
    56281 tcaacaacta catgagcatc gcgctcaatg cgctttcggc agaagccgcc gttttgtgcg
    56341 aagccctgaa tcttcccttc gatgttgccg tcaaagtgat gagcggtacc gccgccggta
    56401 aaggtcactt caccacttcc tggccaaaca aagtcctcag cggagatctt tctcccgcct
    56461 tcatgatcga tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc aaccagctgc
    56521 atgtgccaat gccgctgggg gccgcctcac gggaggttta tagccaggcg cgcgcagcgg
    56581 gtcgcggtcg ccaggactgg tccgccattc tggaacaggt ccgtgtcagt gccgggatga
    56641 ctgccaaagt aaaaatgtaa cgactggata aaggaataaa tgaatgaata agtacaccat
    56701 caacgacatt acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc
    56761 catgcgcatg atgtttgccg cggctggggc acccgctccg gtagccgata gcgttttaac
    56821 tgatttcaaa gttaacgctg caaaggccct ctcgccttat gcctcggcga ttctggtaga
    56881 tcaacaattc tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa gttgcgccat
    56941 gattgtcgcc gccgatgagt tcattcctgg caacggtatt ccggtcgata gcgtggttat
    57001 tgaccgcaaa atcaatccgc tacagatcaa acaagacggt ggcaaagcct taaaactgct
    57061 ggtgctgtgg cgtagtgatg aagatgcgca gcaacgtctg gatatggtga aagagttcaa
    57121 cgaactgtgc cactcacacg gtctggtaag catcattgag ccagtcgtcc gcccaccgcg
    57181 tcgtggcgat aaattcgatc gcgaacaagc gatcatcgat gccgccaaag agctgggcga
    57241 cagtggcgct gacctctaca aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga
    57301 gcttctctgt gcttcacaac gtctgaatga ccatatcaat atgccatggg tgatcctctc
    57361 ttccggtgtc gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc
    57421 atcgggattc ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac cagacaacga
    57481 gctgatgctg cgtgacgttt gcgcaccgaa attacaacaa cttggcgata tcgtcgacga
    57541 aatgatggct aaacgccgct aagatgagga taaaaggaat gaaatggttt aacaccctaa
    57601 gccacaaccg ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg
    57661 tagtgccgac tggttttggc tggttaggca ataaagggca aatcaaagaa gagatgggca
    57721 cccatctgtg gatcaccgct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc
    57781 gacctggcgc ttactcgttg gttgatcacg gtatcaaagc catgaacggc gcactgcgtg
    57841 ataaaaaata tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca
    57901 aacagggcta tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc
    57961 acccggaagc gcgcaagctg ctcgattaca ccattgaaat tatcgagaaa tatttctgga
    58021 gcgaagaaga gcagatgtgc ctggaatcct gggacgaagc cttcagcaaa accgaagagt
    58081 accgcggcgg caatgccaat atgcacgcgg tggaagcttt cttgattgtt tatgacgtca
    58141 ctcacgacaa aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg
    58201 tcgccagaaa taatcattat cgcgttaacg aacacttcga tacccagtgg aatccgctgc
    58261 cggattacaa caaagataac ccggcgcatc gcttccgcgc gtttggaggt acaccaggcc
    58321 actggatcga atggggccgt ttaatgctgc acatccatgc tgccctggaa gcccgttgcg
    58381 aacaaccacc agcatggctg ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg
    58441 atgcctgggc acccgatggc gcggacggga ttgtttatac cgttgactgg gaaggaaaac
    58501 cggtggtccg cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg gcctacgcgc
    58561 tctacaccgt cactggcgat cgccagtatg aaacctggta tcaaacatgg tgggagtact
    58621 gcattaagta cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca
    58681 ataaggtcac caccaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc
    58741 tggtgatccc gcgtatcccg ttagcgcccg gcatggctcc agcggttgca gcgggtctgc
    58801 tggatattaa tgcgaaataa ctgatctgaa aatagggtgc gggttttccc ctcaccctaa
    58861 ccctctcccc aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg acatcagcct
    58921 cacgggaggc tgtatgtcgt taattaaggt gccttgtatg caaatcacca atatgcattg
    58981 cagtggacag accgtttcac ttaccgcggg ggattatcac gcgaccattg tgaccgtcgg
    59041 tgctggtctg gctgaattaa cctttcaggg ttgtcattta gtcatcccgc acaaacccga
    59101 agagatgccg ctggcgcatc tgggtaaagt gcttattccg tggccgaacc ggatcgccaa
    59161 cggctgttac cgctatcagg ggcaggaata tcagttaccc attaatgaac atagttcaaa
    59221 agccgctatt cacgggctgc ttgcctggcg agactggcaa attagtgaat taaccgccac
    59281 cagcgttacc ctgacagctt ttctgccgcc cagttacggc tatccgttta tgctggcttc
    59341 gcaggttgtt tattcgctca atgcacgcac gggtctttcc gttgagatcg caagtcagaa
    59401 tattggtact gtgaccgcac cctatggtgt gggtattcat ccttatttaa cctgcaactt
    59461 aacatctgtt gatgaatatc tgttccagct tccggcaaac caggtttatg ccgttgatga
    59521 acacgtaaat ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc
    59581 gaaaaaaata gccgcgacaa aaatcgacca cacctttaaa accgctaatg atctgtggga
    59641 aataaccatt acccatccac aacaggcgct ttcggttagc ctttgttgcg atcaaccctg
    59701 ggtacaaatt tatagcggtg aaaaattaca gcgtcaggga ctggcagttg aacctatgag
    59761 ttgcccgcct aacgccttta attcaggtat cgacttatta ttactggaac caggaaaacc
    59821 gcaccgcctg ttttttaaca tttacggaca gagaaaataa cttgtattga ttagcagtca
    59881 aaatcattga agaacatttc actcgctaaa ggcaacatta tgtatcttca atttattaag
    59941 ttaaaaacag ctcagcttct gtaaagtttt tatatatcag ttttatgaga atcattttac
    60001 ataagcctgt aataacagga aggcaacaat ctcgttctca actctatgga gattagttat
    60061 ggatacgcca cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac
    60121 cctacatttt caacaacgtc ttattttaac ccatagcaaa gataatcctt gtttatggat
    60181 tggctcaggt atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact
    60241 acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat
    60301 tcatttcagc cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg
    60361 ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct
    60421 tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct
    60481 gcgtggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac
    60541 ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt
    60601 cttcccacag cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg
    60661 ctatatgaac ttcgacttta gtgccccgga ataccatgaa ctggcgctgt gggaagacaa
    60721 agcaacgctg cgttttgaat gtgctgacac atacatttcc ctgctggaaa aattaaccgc
    60781 cctgctggga cgccagccag aactgcccga ctggatttat gacggagtaa cgctcggcat
    60841 tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa
    60901 ggtcaacggc atctgggcgc aggactggtc cggtattcgt atgacctctt ttggcaaacg
    60961 cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa
    61021 gcagtggaat caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga
    61081 taaagatctc tgcgaagaag cggcacaaca cggctatctg gcaaaagatg cctctggcgg
    61141 tgactatctg gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga
    61201 agcctacgcc tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg
    61261 ctggatggct gacttcggcg agtatctgcc caccgacacg tacctgcata acggcgtcag
    61321 tgccgaaatt atgcataacg cctggcctgc gctgtgggcg aagtgtaact acgaagccct
    61381 tgaagaaacg ggcaagctcg gcgagatcct tttctttatg cgcgccggtt ctaccggtag
    61441 ccagaaatac tccaccatga tgtgggcggg cgaccagaac gtcgactgga gtctcgacga
    61501 tggcctggcg tcggttgtcc cggcggcgct gtcgctggca atgaccggac atggcctgca
    61561 ccacagcgac attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct
    61621 gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg
    61681 tcctggcgac aactggcagt ttgacggcga cgcagaaact atcgcccatt ttgcccgtat
    61741 gacctccgtc ttcaccaccc tgaaacctta cctgaaagag gctgtcgcgc tgaatgcgaa
    61801 gtccggcctg ccggttatgc gcccgctgtt cctgcattac gaagacgatg cgcacactta
    61861 caccctgaaa tatcagtacc tgttaggtcg cgacattctg gtcgctccgg tgcatgaaga
    61921 aggccgtagc gactggacgc tctatctgcc ggaggataac tgggtccacg cctggacggg
    61981 tgaagcgttc cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt
    62041 ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg ttaaaaagca tctaatcaca
    62101 tccgcccgtg gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga
    62161 agatccagca actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct
    62221 ggcctctaac atcctgctgg atatcggtac gctttatctt ttgaagtttt ataccgacgt
    62281 tctggggctg ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc
    62341 gtttaccgat atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg
    62401 taagttccgt ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa
    62461 ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat
    62521 gctttacgga ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat
    62581 caccaaaaac cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct
    62641 gggcctgctg ctgtgcacgg tgggattcgt gccagttatg aatcttatcg aaggtaatca
    62701 gaaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc tgtttatgtg
    62761 gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca atccggcgca
    62821 aaagcctggc ctgttgcaat ctttccgcgc aattgccggt aaccgcccac tgttcattct
    62881 gtgtattgcc aacctctgca ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta
    62941 ttacacccag tacgtactta acgatcccat cctgttgtcg tatatgggat ttttcagcat
    63001 gggctgtatt ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa
    63061 gaaggtctat atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt
    63121 cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgtt
    63181 tgttaacagc ctgaactggg cgctggtttc cgacaccgtc gagtacggcg agtggcgcac
    63241 cggcgtgcgt tcggaaggca cggtctacac cggcttcacc ttctttcgca aagtgtctca
    63301 ggcgctggct ggtttcttcc ccggctggat gctgacgcaa attggctatg tgccgaacgt
    63361 cgcacaggct gaccacacca ttgaagggtt acgccagttg atcttcatct acccaagcgc
    63421 actggcggta gtcaccattg tggcgatggg ttgcttctac agcctgaacg agaagatgta
    63481 tgtccgcatt gtggaagaga tagaagcccg taaacgcacg gcgtaataat aataaacgac
    63541 gccctgcggg gcgttataag gagtgattat gtctgaccat aatccactga cattaaaact
    63601 gaatctgcgg gaaaaaatcg cctatggtat gggcgacgtc ggttcgaatt taatgctctg
    63661 catcggtact ctgtatctcc tcaaatttta taccgatgaa ttagggatgc ctgcttacta
    63721 tggcgggatt atctttctgg tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg
    63781 atttttactc gactcgcgga aaaatattgg gcctaaaggc aaatttcgtc cctttatttt
    63841 atatgcagca gttccggcgg cgttaattgc aacgctccag tttatcgcca ccaccttttg
    63901 tttgccggtt aaaacgacga ttgccaccgc gctgtttatg atgtttggac tttcatacag
    63961 tctgatgaac tgctcgtatg gtgcgatgat cccggcaatt accaaaaacc cgaatgagcg
    64021 cgcgcagctc gcggcttacc gccagggtgg tgcaaccata gggctattga tttgtaccgt
    64081 ggcgtttatt ccgttgcagt cgcttttttc tgactcaacc gtcggttatg cctgtgcggc
    64141 acttatgttc tccattggcg gctttatttt tatgatgctg tgctacagag gcgtcaaaga
    64201 gcattatgtg gacacagcgc caaccggaca taaagccagt attctcaaat ctttttgcgc
    64261 gatatttcgt aatccaccat tgctggtttt atgcattgct aacctgtgta ctctggcggc
    64321 atttaatatc aaactggcga ttcaggtcta ttacacccag tatgtgctga atgatattaa
    64381 tttattgtcg tggatgggat tcttcagtat gggatgcatc ctcgtcggcg tattactggt
    64441 gccagtaact gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca tggtgctgtg
    64501 ggcggtgggt gatatactga attatttctg gggaagtaac tctttcactt tcgtcatgtt
    64561 ctcttgtgtc gccttttttg gcacggcctt tgtaaacagc ctgaactggg cactggtacc
    64621 agataccgta gattacggtg aatggaaaac cggtattcgt gccgaaggtt ctgtttatac
    64681 cggttatacc ttctttcgta aaatttctgc cgcacttgct ggcttcttgc caggcattat
    64741 gctgacgcaa attggttatg ttcccaacat cgcgcaaagc gatgcgacat tacagggttt
    64801 gcgtcagctc atttttatct ggccttgtgc actggcaatt attgctgcat tgacgatggg
    64861 attcttttac acactcaatg aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg
    64921 caaaaataaa gagatggcga cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat
    64981 agccgctatt tccatcctgg tgggtggcgg cctccctacg tttaaaaaat ggacttatta
    65041 tgaaaaagat taatgcaata attttattat cttctctgac ctctgcctcg gtatttgctg
    65101 gcgcatacgt cgaaaacagg gaggcatata atcttgcctc cgatcagggc gaggttatgc
    65161 tacgtgtggg ttataacttc gatatgggcg cgggtattat gttaaccaat acctacacct
    65221 ttcagcgaga agatgaacta aaacatggct ataatgaaat tgaaggttgg tatcctttat
    65281 tcaaaccaac cgataaatta accatccagc ccggtggctt aattaatgat aagagtatcg
    65341 gttcgggtgg tgcagtgtat ctggacgtca actataaatt tgtaccatgg tttaatctga
    65401 cagtacgaaa tcgctataac cataataact atagttcaac agatttgagc ggggaactgg
    65461 ataataatga cacctatgaa attggcacct actggaattt taaaatcacc gataaatttt
    65521 cctatacatt tgagccacat tacttcatgc gagtgaatga ctttaatagc agcaatggaa
    65581 aagatcatca ctgggaaatc actaatacct tccgttatcg cattaatgaa cactggctgc
    65641 cttatttcga attacgttgg ttagatcgta atgtcgaacc gtaccaccgc gagcaaaacc
    65701 agattcgtat cgggacgaag tatttcttct gatgtgaaac tgccggatgc ggcgtaaacg
    65761 ccttatccgg cctacggttg atacggtttt cgttggccgg attaagcaca tccggccatt
    65821 aatcaagctt cagccgccag catctctttc ttacgttggc taattcgctt aaccagcatt
    65881 acggaaacca ccgcaccaca gaatgcgaag caggccataa tgccaaacac gattttgtag
    65941 ccaataatcc ccggatttaa atccagaatg tagccataca gactgaagca gaacatcgcc
    66001 ggagcgtaac caataaagct acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt
    66061 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
    66121 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
    66181 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
    66241 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
    66301 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
    66361 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt aaaagcgata
    66421 agccagatag ttttgtcttt cagcaccgag gtcatactgg tgtttttcga tgctccgtct
    66481 tcttttttca cctcgacgga cggtgcctct tctttgtcat tcagaacaaa gaaaatgata
    66541 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acccaataag
    66601 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
    66661 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
    66721 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
    66781 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
    66841 gtcgcaatga gtgagaatga catggtgtat ctgcgtggta gtttatcggc aaaataaata
    66901 gacagaaaaa aacccactgt cgtgacaaat gagtttaccg acatagcatt accaatttga
    66961 ccattggtca aatggaaata ttcctgcat
//
LOCUS       DAFAVF010000007        64088 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFAVF010000007 DAFAVF010000000
VERSION     DAFAVF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.007"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            52..615
                     /gene="srlA"
                     /locus_tag="LGK42_000428"
                     /gene_synonym="gutA"
     CDS             52..615
                     /gene="srlA"
                     /locus_tag="LGK42_000428"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK2656717.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            612..1571
                     /gene="srlE"
                     /locus_tag="LGK42_000429"
                     /gene_synonym="gutE"
     CDS             612..1571
                     /gene="srlE"
                     /locus_tag="LGK42_000429"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK2656718.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1582..1953
                     /gene="srlB"
                     /locus_tag="LGK42_000430"
                     /gene_synonym="gutB"
     CDS             1582..1953
                     /gene="srlB"
                     /locus_tag="LGK42_000430"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK2656719.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1957..2736
                     /gene="srlD"
                     /locus_tag="LGK42_000431"
     CDS             1957..2736
                     /gene="srlD"
                     /locus_tag="LGK42_000431"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBK2656720.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2841..3200
                     /gene="gutM"
                     /locus_tag="LGK42_000432"
     CDS             2841..3200
                     /gene="gutM"
                     /locus_tag="LGK42_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBK2656721.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3267..4040
                     /gene="srlR"
                     /locus_tag="LGK42_000433"
                     /gene_synonym="gutR"
     CDS             3267..4040
                     /gene="srlR"
                     /locus_tag="LGK42_000433"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBK2656722.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4033..4998
                     /gene="gutQ"
                     /locus_tag="LGK42_000434"
     CDS             4033..4998
                     /gene="gutQ"
                     /locus_tag="LGK42_000434"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBK2656723.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4995..6509)
                     /gene="norR"
                     /locus_tag="LGK42_000435"
     CDS             complement(4995..6509)
                     /gene="norR"
                     /locus_tag="LGK42_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBK2656724.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6696..8135
                     /gene="norV"
                     /locus_tag="LGK42_000436"
     CDS             6696..8135
                     /gene="norV"
                     /locus_tag="LGK42_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBK2656725.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8132..9265
                     /gene="norW"
                     /locus_tag="LGK42_000437"
     CDS             8132..9265
                     /gene="norW"
                     /locus_tag="LGK42_000437"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBK2656726.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9491..11743)
                     /gene="hypF"
                     /locus_tag="LGK42_000438"
     CDS             complement(9491..11743)
                     /gene="hypF"
                     /locus_tag="LGK42_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBK2656727.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11896..12423)
                     /gene="hydN"
                     /locus_tag="LGK42_000439"
     CDS             complement(11896..12423)
                     /gene="hydN"
                     /locus_tag="LGK42_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBK2656728.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12572..13582)
                     /gene="ascG"
                     /locus_tag="LGK42_000440"
     CDS             complement(12572..13582)
                     /gene="ascG"
                     /locus_tag="LGK42_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBK2656729.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13842..15299
                     /gene="ascF"
                     /locus_tag="LGK42_000441"
     CDS             13842..15299
                     /gene="ascF"
                     /locus_tag="LGK42_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBK2656730.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            15308..16732
                     /gene="ascB"
                     /locus_tag="LGK42_000442"
     CDS             15308..16732
                     /gene="ascB"
                     /locus_tag="LGK42_000442"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBK2656731.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16891..17361)
                     /gene="hycI"
                     /locus_tag="LGK42_000443"
     CDS             complement(16891..17361)
                     /gene="hycI"
                     /locus_tag="LGK42_000443"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBK2656732.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17354..17764)
                     /gene="hycH"
                     /locus_tag="LGK42_000444"
     CDS             complement(17354..17764)
                     /gene="hycH"
                     /locus_tag="LGK42_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBK2656733.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17761..18528)
                     /gene="hycG"
                     /locus_tag="LGK42_000445"
     CDS             complement(17761..18528)
                     /gene="hycG"
                     /locus_tag="LGK42_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBK2656734.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIIPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18528..19070)
                     /gene="hycF"
                     /locus_tag="LGK42_000446"
     CDS             complement(18528..19070)
                     /gene="hycF"
                     /locus_tag="LGK42_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBK2656735.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19080..20789)
                     /gene="hycE"
                     /locus_tag="LGK42_000447"
     CDS             complement(19080..20789)
                     /gene="hycE"
                     /locus_tag="LGK42_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBK2656736.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20807..21730)
                     /gene="hycD"
                     /locus_tag="LGK42_000448"
     CDS             complement(20807..21730)
                     /gene="hycD"
                     /locus_tag="LGK42_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBK2656737.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21733..23559)
                     /gene="hycC"
                     /locus_tag="LGK42_000449"
     CDS             complement(21733..23559)
                     /gene="hycC"
                     /locus_tag="LGK42_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBK2656738.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23556..24167)
                     /gene="hycB"
                     /locus_tag="LGK42_000450"
     CDS             complement(23556..24167)
                     /gene="hycB"
                     /locus_tag="LGK42_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBK2656739.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24292..24753)
                     /gene="hycA"
                     /locus_tag="LGK42_000451"
     CDS             complement(24292..24753)
                     /gene="hycA"
                     /locus_tag="LGK42_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBK2656740.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            24965..25315
                     /gene="hypA"
                     /locus_tag="LGK42_000452"
     CDS             24965..25315
                     /gene="hypA"
                     /locus_tag="LGK42_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBK2656741.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25319..26191
                     /gene="hypB"
                     /locus_tag="LGK42_000453"
     CDS             25319..26191
                     /gene="hypB"
                     /locus_tag="LGK42_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBK2656742.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26182..26454
                     /gene="hypC"
                     /locus_tag="LGK42_000454"
     CDS             26182..26454
                     /gene="hypC"
                     /locus_tag="LGK42_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBK2656743.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDKNGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26454..27575
                     /gene="hypD"
                     /locus_tag="LGK42_000455"
     CDS             26454..27575
                     /gene="hypD"
                     /locus_tag="LGK42_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBK2656744.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27572..28582
                     /gene="hypE"
                     /locus_tag="LGK42_000456"
     CDS             27572..28582
                     /gene="hypE"
                     /locus_tag="LGK42_000456"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBK2656745.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28656..30734
                     /gene="flhA"
                     /locus_tag="LGK42_000457"
     CDS             28656..30734
                     /gene="flhA"
                     /locus_tag="LGK42_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBK2656746.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(30771..31124)
                     /locus_tag="LGK42_000458"
     CDS             complement(30771..31124)
                     /locus_tag="LGK42_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBK2656747.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(31156..31335)
                     /locus_tag="LGK42_000459"
     CDS             complement(31156..31335)
                     /locus_tag="LGK42_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656748.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            31411..33972
                     /gene="mutS"
                     /locus_tag="LGK42_000460"
     CDS             31411..33972
                     /gene="mutS"
                     /locus_tag="LGK42_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK2656749.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            34078..34734
                     /gene="pphB"
                     /locus_tag="LGK42_000461"
     CDS             34078..34734
                     /gene="pphB"
                     /locus_tag="LGK42_000461"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBK2656750.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATSDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(34785..35552)
                     /locus_tag="LGK42_000462"
     CDS             complement(34785..35552)
                     /locus_tag="LGK42_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2656751.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            35748..36656
                     /locus_tag="LGK42_000463"
     CDS             35748..36656
                     /locus_tag="LGK42_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK2656752.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            36653..37915
                     /locus_tag="LGK42_000464"
     CDS             36653..37915
                     /locus_tag="LGK42_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HBK2656753.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            37912..38550
                     /locus_tag="LGK42_000465"
     CDS             37912..38550
                     /locus_tag="LGK42_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBK2656754.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            38555..39331
                     /locus_tag="LGK42_000466"
     CDS             38555..39331
                     /locus_tag="LGK42_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBK2656755.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            39420..40784
                     /locus_tag="LGK42_000467"
     CDS             39420..40784
                     /locus_tag="LGK42_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBK2656756.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(40823..41059)
                     /locus_tag="LGK42_000468"
     CDS             complement(40823..41059)
                     /locus_tag="LGK42_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656757.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(41070..42497)
                     /locus_tag="LGK42_000469"
     CDS             complement(41070..42497)
                     /locus_tag="LGK42_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK2656758.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(42497..43090)
                     /locus_tag="LGK42_000470"
     CDS             complement(42497..43090)
                     /locus_tag="LGK42_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK2656759.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALRDMPNVETHLVMSKWAKTTIE
                     LETPYSAHDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            43237..43644
                     /locus_tag="LGK42_000471"
     CDS             43237..43644
                     /locus_tag="LGK42_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK2656760.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(43765..44757)
                     /gene="rpoS"
                     /locus_tag="LGK42_000472"
     CDS             complement(43765..44757)
                     /gene="rpoS"
                     /locus_tag="LGK42_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBK2656761.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(44820..45959)
                     /gene="nlpD"
                     /locus_tag="LGK42_000473"
     CDS             complement(44820..45959)
                     /gene="nlpD"
                     /locus_tag="LGK42_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBK2656762.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(46099..46725)
                     /gene="pcm"
                     /locus_tag="LGK42_000474"
     CDS             complement(46099..46725)
                     /gene="pcm"
                     /locus_tag="LGK42_000474"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBK2656763.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(46719..47480)
                     /gene="surE"
                     /locus_tag="LGK42_000475"
     CDS             complement(46719..47480)
                     /gene="surE"
                     /locus_tag="LGK42_000475"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBK2656764.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(47461..48510)
                     /gene="truD"
                     /locus_tag="LGK42_000476"
     CDS             complement(47461..48510)
                     /gene="truD"
                     /locus_tag="LGK42_000476"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBK2656765.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(48507..48986)
                     /gene="ispF"
                     /locus_tag="LGK42_000477"
     CDS             complement(48507..48986)
                     /gene="ispF"
                     /locus_tag="LGK42_000477"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBK2656766.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(48986..49696)
                     /gene="ispD"
                     /locus_tag="LGK42_000478"
     CDS             complement(48986..49696)
                     /gene="ispD"
                     /locus_tag="LGK42_000478"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBK2656767.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQIPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(49715..50026)
                     /gene="ftsB"
                     /locus_tag="LGK42_000479"
     CDS             complement(49715..50026)
                     /gene="ftsB"
                     /locus_tag="LGK42_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBK2656768.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(50220..50543)
                     /locus_tag="LGK42_000480"
     CDS             complement(50220..50543)
                     /locus_tag="LGK42_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBK2656769.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(50593..51198)
                     /gene="cysC"
                     /locus_tag="LGK42_000481"
     CDS             complement(50593..51198)
                     /gene="cysC"
                     /locus_tag="LGK42_000481"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBK2656770.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(51198..52625)
                     /gene="cysN"
                     /locus_tag="LGK42_000482"
     CDS             complement(51198..52625)
                     /gene="cysN"
                     /locus_tag="LGK42_000482"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBK2656771.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(52627..53535)
                     /gene="cysD"
                     /locus_tag="LGK42_000483"
     CDS             complement(52627..53535)
                     /gene="cysD"
                     /locus_tag="LGK42_000483"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBK2656772.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            53787..54824
                     /gene="iap"
                     /locus_tag="LGK42_000484"
     CDS             53787..54824
                     /gene="iap"
                     /locus_tag="LGK42_000484"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBK2656773.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTASDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSQGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRFDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   54907..55423
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=54907..54935
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(55520..55813)
                     /gene="cas2"
                     /locus_tag="LGK42_000485"
     CDS             complement(55520..55813)
                     /gene="cas2"
                     /locus_tag="LGK42_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBK2656774.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(55810..56733)
                     /gene="cas1e"
                     /locus_tag="LGK42_000486"
     CDS             complement(55810..56733)
                     /gene="cas1e"
                     /locus_tag="LGK42_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HBK2656775.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPTIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(56730..57380)
                     /gene="cas6e"
                     /locus_tag="LGK42_000487"
     CDS             complement(56730..57380)
                     /gene="cas6e"
                     /locus_tag="LGK42_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBK2656776.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(57362..58108)
                     /gene="cas5e"
                     /locus_tag="LGK42_000488"
     CDS             complement(57362..58108)
                     /gene="cas5e"
                     /locus_tag="LGK42_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBK2656777.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(58119..59174)
                     /gene="cas7e"
                     /locus_tag="LGK42_000489"
     CDS             complement(58119..59174)
                     /gene="cas7e"
                     /locus_tag="LGK42_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBK2656778.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLSLLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(59189..59725)
                     /gene="casB"
                     /locus_tag="LGK42_000490"
     CDS             complement(59189..59725)
                     /gene="casB"
                     /locus_tag="LGK42_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBK2656779.1"
                     /translation="MSIVKDGHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTCL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(59722..61284)
                     /gene="casA"
                     /locus_tag="LGK42_000491"
     CDS             complement(59722..61284)
                     /gene="casA"
                     /locus_tag="LGK42_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HBK2656780.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGIRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQDDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHRFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADGLLSKWNKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(61382..64081)
                     /locus_tag="LGK42_000492"
     CDS             complement(61382..64081)
                     /locus_tag="LGK42_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBK2656781.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPTSSLSLFHSRFAFSDRQRIETKTLARFGKE
                     GSSQRAGKALISTQVLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPDEEWFGSAMRNSAYIYPDHGRIWLTQRVLRQQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYTTGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDNESCGYSATEGLIGKVG"
BASE COUNT        15071 a        17506 c        16656 g        14855 t
ORIGIN      
        1 agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac aatgatagaa
       61 accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt
      121 accgggatgg tgaccggcat tcttccgctg ttgattagcc tgctggttat catgaacgca
      181 ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac
      241 cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg
      301 accttaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc
      361 tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt
      421 gtttatcttg gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg
      481 gtgagttatc tgctggttgg tctggtcacc aatttcttcc gcggctgggt gaccgatctg
      541 accaccgcca tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcacctggca
      601 ggagcaacat catgacgcat attcggatcg aaaaaggaac gggtggctgg ggcggcccgc
      661 ttgagctgaa agccacgccg ggaaaaaaaa tcgtctatat caccgccggt acccggcctg
      721 cgattgttga caaactggca cagcttactg gctggcaggc tattgacgga tttaaagaag
      781 gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc acattacgct
      841 gcggcatcta tccgaaacga cgtattccca ccattaatat ccactcgacg ggcaagtccg
      901 gtccgctggc gcagtacatt gtggaagata tttatgtctc tggcgtaaaa gaagaaaaca
      961 tcactgtagt gggtgatgcg acaccacaac cctcttccgt gggccgtgac tatgacacca
     1021 gtaagaaaat caccgaacaa agcgatggtt tactggcgaa ggtgggaatg ggcatggggt
     1081 ccgccgttgc ggtgctgttt caatctggtc gtgacaccat cgacactgta ttaaaaacca
     1141 ttctgccgtt tatggcattc gtctcggcgc tcattggcat cattatggct tctggccttg
     1201 gtgactggat tgcccacggt cttgctccgc tggcgagcca tccactgggt ctggtcatgc
     1261 tggcgctcat ctgctccttc ccactgcttt cacctttcct cggcccaggc gcagttatcg
     1321 cacaggttat cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc
     1381 tggctttacc ggcactgttt gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg
     1441 gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgtactgg
     1501 tgagccgctt tttaaccggc gcgccgactg tactgatcgc ctggtttgtc tccggtttta
     1561 tctatcaata gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtatcggc
     1621 gcgagtgcca ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcca
     1681 gcggacctcg aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat
     1741 cccggtttgc aattttcact cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg
     1801 gcggaagaca accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa
     1861 gcggaatttc cgggcactgt ccatgtggca ggccctgtcc ccgacgatat cgcgccggga
     1921 tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc
     1981 ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc tggctgccga ggggtatcgc
     2041 gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc
     2101 gaatatggtg aaagtatggc gtacggtttt ggtgctgacg ccactagcga gcaaagcgtt
     2161 ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg tggatttgct ggtctacagc
     2221 gccggaatag caaaagcagc ctttatcagc gacttccagc tcggcgattt tgaccgttcg
     2281 ctacaggtga atctggtggg ttatttcctg tgtgcgcgtg aattttcgcg tttgatgatc
     2341 cgcgacggga ttcaggggcg cattattcag atcaactcga aatccggcaa agtgggcagc
     2401 aaacacaact ctggctacag cgcagcgaaa tttggtggcg tcgggctgac tcaatcactg
     2461 gcgctggatc tggcggagta cggcattacg gtgcattcac tgatgctcgg taacctgctg
     2521 aaatcgccga tgttccagtc actgttgcca caatacgcga ccaagctggg tatcaaaccg
     2581 gatcaagtcg agcagtatta catcgacaaa gtaccgctca aacgcggctg cgattatcaa
     2641 gatgtgctga atatgctgct gttctacgcc agtcctaagg cgtcgtactg caccggacag
     2701 tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat ccattaagga
     2761 tctccgtgag actatagaat gcctgatgcg ctacgctcat caggcataca ggacttccgc
     2821 cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt atcgcctggt
     2881 gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac
     2941 tatgccagca agggcgggtt ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg
     3001 tggtcgccat cgcgctggac gatcagcagc gcatcgtcga caccttgttt atgaaaggac
     3061 tgaccgtctt cgcccgaccg caaaaaattc ccgcaattac cggtatgcat gcgggtgatt
     3121 tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta tcattggcgc
     3181 ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag ttatcatttt
     3241 gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc gccattctgg
     3301 agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca
     3361 caggcacaac cattcgcaaa gatctggtca ttctggaaca tgccggaacc gtcattcgta
     3421 cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat cataaaacac
     3481 tcatcaacac ccacaagaaa gagctgattg cagaagctgc cgttagtttt atccatgatg
     3541 gcgattcgat cattcttgat gctggcagta ccgttttgca gatggttccc ctgctctcgc
     3601 gctttaataa catcacggtg atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac
     3661 tggataacga acaaactatc ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat
     3721 ttcacggaca gctggcagag aatgccttcg aacatttcag tttcgataaa ttgtttatgg
     3781 gcaccgacgg catcgatctc aatgcgggcg taaccacctt caacgaggtt tataccgtca
     3841 gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca tcaaagtttg
     3901 gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg attaccgacg
     3961 caggtatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat gtgatcataa
     4021 ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt aatgctggag
     4081 ttgcaggaag caagccgttt accggaacgt ctgggcgatg attttgttcg cgccgccaat
     4141 atcatcctgc actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc gggccacatt
     4201 ggtaagaaaa tcgccgcaac acttgccagt accggcacac cggctttttt tgtccatccg
     4261 gcagaagcac tgcacggcga tctggggatg attgaaagcc gcgatgtgat gctgtttatc
     4321 tcttactccg gtggcgcgaa ggaactggat ctgattattc cgcgtctgga agataaatct
     4381 atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc ggcaaaagcg
     4441 gtgctggata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc gacctccagc
     4501 accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcgga
     4561 tttaatgaag aagattttgc ccgctcccac ccagccgggg cactgggcgc tcgcttgctg
     4621 aataaagtgc atcatctgat gcgccgtgac gatgccatcc cacaggtggc gttaaccgcc
     4681 agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc tggggctggt ggcggtatgt
     4741 gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg ctggctggtt
     4801 ggcggcggcg cactcaccac gccagttaat gaagcgatga cgacaggcgg caccacatta
     4861 caggcacaaa gtcgcgccat cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc
     4921 gcaccggtgg tggatgaaaa cggcaaactc accggcgcaa ttaacctgca ggatttctat
     4981 caggccggga ttatttaatc cttcaatccc agacgtttcg ccagccgatg caggttggcg
     5041 acgtcggttt ccagcatccg cgcgcaggca gcccagttgt gatgattttg tgccagtgcc
     5101 tggcgaatgg tttcacgctg gaacgcttct gtcgcttcac gcaggttttg cttaacaacg
     5161 ggcaccgccg ccgcttctgg cggcggcaac gtcacctcag gaaaagcaaa atgttgtgcc
     5221 tcaagaatca cttcatcgcc gctgcgagtg gctctcgcca gcactaccgc ccgatgaata
     5281 gcatgttcca gttcgcgcac gttccccgga aaacgatagt gttgcagtaa atttcgcgct
     5341 ccggcactta ataccacgcg ggagagcccc agccgcaaac gacactgctc gcagaaatac
     5401 cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga aagtggaaac
     5461 acgctcaggc gatgaaacaa atcggcgcgg aatcgccctg ccagcacctc ttcgcgtaag
     5521 tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aactacggtc atcgccaacg
     5581 cgctgaatat cgccatactg caacaccctc agcagcttgg cctgcaatgc caacgacaac
     5641 tcgccgatct catcgagaaa cagagtgccg ttatccgcca tttcaaactt cccgctgcgg
     5701 ttactgatag cgccagtaaa cgctcctttc acatgcccga acaactcact ttccgccaca
     5761 ctttccggca gtgcggcaca gttgagatag accagcggat tcaccgcccg tggcgaggct
     5821 tcatgaatcg ctttcgccac cagctcctta ccggttcccg tctcaccgct gatcaggacg
     5881 ttgagatcgg acgccgccac aatctcaatc tcttttttca gttgcgtcat gccaggggac
     5941 aagccaatca tctgcgtctg tttcaccgct tcaaacggcg tggcatcgcc tggcatcata
     6001 ttctggcttt ccagttgttc aatcagcaac gcattgctta acgctcccgc cgccagcgca
     6061 gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact gatcgggctg catcccgtcg
     6121 agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg gcagaccaac gcaggcgtga
     6181 accttcagac tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct
     6241 gcaggaaagc gcaccacgtc cccggcgcgg gcaatcgctt caagccgtgg atgcccttcc
     6301 agcgcaaagc gtctaccgag tacatccttt gccagaccgt cgatggcaag cggaataaac
     6361 tgccgcgaat cgtaacgtag caacgcagac gcatcgcact ccagcacctg acgtagcgtg
     6421 gtgatcaggc gctgaaaacg atcctgatga ccaatcccac gctgcaattc gatggcgata
     6481 ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg actatagata
     6541 ttgtcatatc gaccatttga ttgatagtca ttttgactac ccattaatga gcatactttt
     6601 atttatatag taaaaacaat cagataaaaa actggcacgc aatctgcaat tagcaagaca
     6661 tctttttaga acacgctgaa taaattgagg ttgctatgtc tattgtggtg aaaaataaca
     6721 ttcattgggt tggtcaacgt gactgggaag tgcgtgattt tcacggcacg gaatataaaa
     6781 cgctgcgcgg cagcagttac aacagctacc tcatccgcga agaaaaaaac gtgctgatcg
     6841 acaccgtcga ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc
     6901 tggcggatat cgattacatc gtgattaacc atgcagaaga ggaccacgct ggggcgctga
     6961 ccgaactgat ggcgcaaatt cccgatacgc cgatctactg tactgccaac gctatcgact
     7021 cgataaatgg tcatcaccat catccggagt ggaattttaa tgtggtgaaa actggcgaca
     7081 cgctggatat cggcaacggc aaacagctca tttttgtcga aacaccaatg ctgcactggc
     7141 cggacagcat gatgacttac ctgacaggcg acgcggtgct gttcagtaac gatgctttcg
     7201 gtcagcacta ctgcgacgag catctgttca acgatgaagt ggatcagacg gagcttttcg
     7261 agcagtgcca gcgttactac gccaatatcc tgacgccgtt cagccgcctg gtaacgccga
     7321 aaattaccga gatcctgggc tttaacttac cagtcgatat gatagccact tcccacggcg
     7381 tggtatggcg cgataacccg acgcaaattg tcgagctgta cctgaaatgg gcggctgatt
     7441 atcaggaaga cagaatcacc attttctacg acaccatgtc gaataacacc cgcatgatgg
     7501 ctgacgctat cgcccagggg attgcggaaa ccgacccacg cgtggcggtg aaaattttca
     7561 acgtcgcccg aagcgataaa aacgaaatcc tgactaatgt cttccgctca aaaggcgtgc
     7621 tggtcggcac ttcgacgatg aataacgtga tgatgccgaa aatcgccggg ctggtggaag
     7681 agatgactgg tttacgcttc cgtaacaaac gcgccagtgc tttcggctct cacggctgga
     7741 gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc
     7801 ttagcctgaa agccaaatgg cgaccagacc aggacgctct ggagttatgt cgtgaacacg
     7861 gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg
     7921 tggttaaaga agaaacctct gccaccacga cggctgacct cggcccacgg atgcagtgca
     7981 gcgtctgcca gtggatttac gatccggcaa aaggcgagcc aatgcaggac gttgcgccag
     8041 gaacgccgtg gagtgaagtc ccggataact tcctctgccc ggaatgctcc ctcggcaaag
     8101 acgtctttga cgaactggca tcggaggcaa aatgagtaac ggcattgtga tcatcggttc
     8161 gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca ctattccatt
     8221 aaccctgatt gccgccgaca gcatggatga gtacaacaaa cctgacctca gccatgttat
     8281 cagtcagggg caacgtgccg atgaccttac ccgccagacg gcgggtgaat ttgccgagca
     8341 gtttaatctg cacctgtttc cacaaacctg ggtgacggat atcgatgccg aagcccgtgt
     8401 ggtgaaaagc cagaataatc agtggcaata cgacaagcta gtactggcaa ccggtgccag
     8461 tgcctttgtc ccgcctgtgc ctgggcgtga gttaatgctg acgttaaata gtcagcaaga
     8521 gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg ttggcggtgg
     8581 tttgattggt agcgaactgg cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat
     8641 cgacaacgct gccagtattc tggcgtcatt aatgccaccg gaagtaagca gccgcttgca
     8701 gcatcggttg acggagatgg gcgttcatct gttgttgaaa tctcagttgc aggggctgga
     8761 aaaaacagat tctggcattc tggcaacgct ggaccgccag cgcagtatcg aagtggatgc
     8821 ggtaattgcc gccaccggac tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac
     8881 gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg atatttacgc
     8941 gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg ttcctccagc cgattcaact
     9001 tagcgcgatg gtgctggcaa aaaatcttct cggcaataac acgccactga aactaccggc
     9061 gatgctggtg aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg aaacccagcg
     9121 tcaggattta cgctggcaaa ttaataccga acgccaggga atggtggcgc gcggcgttga
     9181 cgatgctgac cagcttcgcg cctttgtggt cagtgaggat cggatgaaag aggcatttgg
     9241 attgttgaaa acattgccga tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc
     9301 tggcgcgtct tatccaacct acggggagcg catgtgttgg tcacatactg cgttcatgtc
     9361 gcatccacct actgtgccca aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac
     9421 ggggacgcat gtgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgtccaaat
     9481 gcaacacgtt ttatccgttc tggacttcac ccgctaacca acgcgccgca gcaataaccc
     9541 cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag agcaatgtga
     9601 aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata accccgccgc
     9661 taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac
     9721 cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccggtgcc tgccagttca
     9781 gccactgctg ccagaaagtg gcgagatcca gttgattgtc caccagcggc attgtcaccg
     9841 gatgcgtcac tccgtggcac gaagctgcga gtgcctccag agcacaagcc gcttcacctt
     9901 cataacttaa cgtggctggc gcacagccca gtgccgccgc cacggcatcg aacaaccgcc
     9961 cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc
    10021 agttttgctg ttgcacactt gctgtttcag aataattctg ccactccggc acaaagcgca
    10081 ggcactgcgc cagcaggttt cgccacggct gcttcgccgc caaatcgcca cccggaagcg
    10141 ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc agacactcgc
    10201 cgccccacaa agcgccgttc tcccccatac cgataccatc gagcgtcaaa gcaatgacat
    10261 caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcgtgg gcatgatgat
    10321 gcagcaccgt ttgcgtcggc agattcattt cgcgcgccca ctggctggag acatagcccg
    10381 gatgtgcgtc atgcacaacg tattgcgggg tgaaatcgta gatgttttgc atcaggcgta
    10441 acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca
    10501 acactgcttg ttcaccgcgc accaggcaga aggtgttttt cagatctgcg ccgagacaca
    10561 gcacaggcgg cacattttta aagcccggag gcaaagccag tgcgtccggt acataccccc
    10621 gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga
    10681 tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt
    10741 cgttgctgat agccggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt
    10801 gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacatcacg ccgacttcgt
    10861 taaggccagg ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa
    10921 tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc tggtaaacct tctgccactg
    10981 gcaagatgac cgccagcggt ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg
    11041 cggtactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa
    11101 tgttgcccat ttttaactgt gcgatagccg cctgtaatgc cgcctcttgt tccgcatgtt
    11161 caccatgact tacccattca agatgtgggc cacactccgg gcaggccacc ggctgggcgt
    11221 ggaagcgacg atcgagcggg tcgcggtact ctttatcaca ggctggacac agcggaaact
    11281 ccgccattac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcgggc
    11341 cgcagtgggt acagttgata aacggataac gataacgccg ttcgcctggg gtattcattt
    11401 cggcaaggca agcagggcaa gtagcggcat ccgggacaat ttgcgtattc atggcacctc
    11461 ccgcgctctg gcggatagtg aactcggtgg gcagttgtga ccagataaac ggctcacgct
    11521 cgacgctatc aatacgcgcc agtggcggac agtgctgatg caattgaaca agaaacgttt
    11581 ccgggtcttc cagcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa
    11641 gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc
    11701 cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat
    11761 catgaattgc gtaacccgtc cagccggaca cgacagcgtc gcatccggca gtcacaggtc
    11821 ggcgataccg cccgcttcgt attctacgaa tatttccggg aattcctttg atgccagaac
    11881 agttctgtaa gatttttaga acatcagcgc cgtacggcgg cgtttttctg cgctcagttg
    11941 ttcaagttta ttacgatcga cacaaattag cgcatgggtc gggcaagccg ccatacacgc
    12001 cgggccgtct tcacgatggt tgcacaggtc gcatttattg gcttcagctt tgtcagcccg
    12061 tacattcagc cccgcgccgc tgttgcggat caccggacgt accaccactt ccatcgcgcc
    12121 atacgggcaa gccaccacgc aggttttgca accaatgcaa cgttcctgca tcacatgaac
    12181 aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc
    12241 acactgacgg cagactgtcg ccgtggaaat gttcacacct ttaatgacat ggattcgcgg
    12301 taaaaaggtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca
    12361 cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt
    12421 catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt
    12481 ttaatttgct gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga
    12541 cactagtgac gatgacatgt gatgacaatg tttatcgcga aagggcaatg agtgagtcgc
    12601 ggcggatcag ttttccgctg aaggttttcg gcggtgagaa atccccgcca tcgagcataa
    12661 aaatcagccg tccaataatt tcctgaatca tctctgttac cggaattttt acgctggaga
    12721 gcgccggaac ggtgtagggg gcaattgcga tatcatcgaa tccgataact gacacctgct
    12781 ctggcaccgc tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc atatcgtcgt
    12841 tactggcaac taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg
    12901 ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat
    12961 gctgcgccag cgcgtcttta tacccagcaa gacgttcaat gctggtgggg gaatccatcg
    13021 agccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcattaa
    13081 agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat
    13141 taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcaatctca tccacgctta
    13201 aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct
    13261 ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgccgc cctttctctt
    13321 ccgccattcg cgcggcatga aacagtaatt cactaaaata aatgccgtga taaagcgtgt
    13381 tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgg gccagcaaat
    13441 ttgggcggta accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga
    13501 cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg
    13561 ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact
    13621 gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc
    13681 acaaatataa aaaatgaaca attaactctc ttgcttattt agtgacaact attaatgatt
    13741 ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtta cgtcgactga
    13801 taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg
    13861 cgctggcacg ctcggtgata gcggcactgg gcggcgttga taacatctcg gcggtcacgc
    13921 actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac agcccgacgt
    13981 taaaaaccat ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta
    14041 tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc
    14101 agcccgcaca gcccgtgggt aaacccaaac tcacgctgcg tcgcattggt gcagggatcc
    14161 tcgatgcgct gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg
    14221 tcaaactgct ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct
    14281 taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg
    14341 catctgccgc catcaaattt aaaaccaata tgtcgctggc gattgccatc gcgggtgtgc
    14401 tggtacatcc gagttttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat
    14461 ttgccctgat tccggtcacc gcggtgaaat acacctacac agtgatcccg gcgctggtga
    14521 tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg gcggtgacca
    14581 aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga
    14641 ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg
    14701 gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga
    14761 ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag
    14821 aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg
    14881 cggtggcgtg gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat
    14941 cagccattat ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc cgcctgaaac
    15001 gtccgcttat cgccagtctt atcagcggct ttatttgcgg cgcggttgcc ggtatggcgg
    15061 ggcttgccag ccactcaatg gcagcgccgg ggctatttac cagcgtgcag ttcttcgatc
    15121 cggcgaatcc aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtcatgctgt
    15181 cgtttatcct cacactgttg ctcggcttcg aggatattcc tgttgaggaa gcgactgccg
    15241 aggcgcgaaa gcatcagagc gtacaaccga ccgtcgccaa agaagtaagt cttaattgag
    15301 gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca
    15361 gtctgaaggt gcgttccgtg aaggtggcaa agggctgacc actgtcgata tgatcccaca
    15421 cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga
    15481 cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc
    15541 cctgatggca gagatgggat tcaaggtttt ccgtacctca attgcctgga gccgtctctt
    15601 tccgcagggc gatgaaatca cgcccaatca gcagggcatt gctttttatc gttctgtctt
    15661 tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc
    15721 gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag
    15781 ccgctacgcc cggacctgct ttgaagcatt tgatggtctg gtgaaatact ggcttacctt
    15841 caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga
    15901 aggtgaaaat caggatcagg tgaaatatca ggccgcgcat caccagctgg ttgccagtgc
    15961 gctagccacc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc
    16021 gggcggtaac ttctaccctt acagttgcaa gccggaagat gtctgggcgg cgctggagaa
    16081 agaccgggaa aacctgtttt ttatcgatgt gcaggcgcgg ggcgcgtatc cggcttactc
    16141 tgcccgcgta ttccgcgaaa aaggggtaac catcgacaaa gcaccgggcg atgatgaaat
    16201 cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcgcgct gcgcctcggc
    16261 ggagatgaac gccaacaata gcagtgcggc gaacgtagtg aaatcgctgc gtaacccgta
    16321 tctgcaagtg agcgactggg gctggggtat tgatccactc ggtctgcgta tcaccatgaa
    16381 catgatgtac gaccgttatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa
    16441 agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga
    16501 acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac
    16561 atggggctgt attgatttag tttccgcctc tacgggtgaa atgagcaaac gctacggctt
    16621 tgtctttgtt gaccgtgacg acgcaggcaa cggtacgctg acgcgcacgc gtaagaaatc
    16681 attctggtgg tataaaaaag tgattgccag taatggggaa gatttagagt aggtaacagt
    16741 gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccat gaaggcccga
    16801 taagcgcagc gcatcgggca atttagcatt tggcatcaga gctggttcac cccatcaaga
    16861 acatccctgt cctgattcct taatgaaaaa ctactcttct tccaccgcta actgcgcgaa
    16921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    16981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    17041 ttcgccaatg tcttctttca actggtcgat aaggtagttc aacggcatgt tatgggtagt
    17101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    17161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    17221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    17281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    17341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    17401 gaatatcatg cagcatactt agcagcattg tactccattc ctgctgctgc gaagtttgcg
    17461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    17521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcgctgccc gcctccagag
    17581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    17641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    17701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    17761 tcatcggata cgcgcctctt ctacaacatg attcagatgg ctgacaatct cgttcagacg
    17821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    17881 caggaagtca tcggcaatct gacgaccgta acgataacct gccaggcggc gtgcttcgcg
    17941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg ccggttgttc
    18001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    18061 aaagccgtac agcgtggcgg caggcgttgg cgggcaaccc ggaatgtaga catctaccgg
    18121 gatgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt
    18181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    18241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    18301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg ataaagtggc
    18361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcggtaaa cgtaggcaga
    18421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    18481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    18541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    18601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcacc attgtgctta
    18661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcga
    18721 cagctgcaca gcacaaaacg ggactgttgc aggaaatctt ctttcttcca caccgccagt
    18781 tcgtactctt gcgacagttt gatcgctgcc gtcgggcaga cttcttcgca gcgtccacag
    18841 aagatgcagc gcccgagatt aaactcccac gcaagctctc ctgtggcgag gtcagtttca
    18901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cgcagccgat gcactgctgc
    18961 gggttctgct ctggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    19021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    19081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    19141 actttgctct tcttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    19201 gggtccaggc taccgataat cagcggcgca tcggaaacgg tgttgccgcg cagcatgtag
    19261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    19321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    19381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcgggccgcc cggcaggtta
    19441 tccagaccgt agtcgatcat gttcagcgcg gtatagactt cattgatacg cactttcaga
    19501 cgggaaataa cgtcgcagcc ctgctcgctg tgcacttcca tcggcagcag gccataacca
    19561 acaaacgggt gatcggcgcg ggtatcgcga gcgtgaccgc tggcacggac catcgggcca
    19621 acgttactga agtcgcgggc aatttccggg tccagacgac caatgccgac ggtgcgctgt
    19681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    19741 tgcgccagct ggcgggtctg gatcatgtca tctttcagca gatcgcgacg aatcccgccg
    19801 atcaggttca ggccgtaggt tttacgcgca ccggtaagga tctctgccat tttcatggag
    19861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcga agccggtaaa gtgacaggcc
    19921 aggccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    19981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg
    20041 gtgctgtgag caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    20101 taacccatac gggtttccgc cagtttttcc atgccgcgat ggacgtagaa caggcggtag
    20161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtgccccgg ttcgtcagaa
    20221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcattgatg
    20281 aactcgtagg tttcagcatc ggtagtcggt gccggacgct gacgataatc catgctgtct
    20341 ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg acgttcatcc
    20401 ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc
    20461 gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac ttcgacgcga
    20521 accgttaccc aacacttggt gcccttctcc atcgacagca cgtaataaac ggcgtaatga
    20581 ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc ctgcttgtag
    20641 tagagaaact ccaccacttc cggcaggtag ttcaccttca cggtcacggt cagctgatct
    20701 ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag cgcggcgaga
    20761 taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca
    20821 aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc ggagtaatat
    20881 caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgacgacca
    20941 gtttgacgat ggcaatcacc agcgccagca gcagcccacc ggcggtgaag gtttccatct
    21001 gtccccacgg gataaatacg ccgacgaaca tctgcagcac caccagctgt ttcaggctga
    21061 taccccattt catgacgcca aagccgctgc cgctgtattc agagagcggg ccttcctgca
    21121 gctcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaggtggcga
    21181 acgcacaggc acaaagcgcc agtaccagcg ggatgctctg gctcagcggc cagtgataaa
    21241 cggtgtcggt gatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac
    21301 caagcagcag catcggttcg accagcacgc caagcatcgc ttcacggctc gcgccgatag
    21361 cggtaaacgg gctaccggta tccagaccag aaatggcaaa gaagaaacgc gcgatggcaa
    21421 agagatacag taaggtgatt aaatcaccca gttgcggcag cggagaaccg acggtcacca
    21481 ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga
    21541 acacccagcc ggaggcatcc gggccaacgc tctggcggcc cagcagtttg aaaatgtcac
    21601 gatactcctg caacacaccc ggcccacgac ggttatgcag acgggcgcgc gccacgcggg
    21661 taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata
    21721 aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac
    21781 cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg gcaccagcga
    21841 caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt tcaccggcat
    21901 ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc
    21961 cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg
    22021 cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc
    22081 aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg gcgcagcaac
    22141 accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc
    22201 acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac
    22261 tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg
    22321 ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg attgatagat
    22381 aacccattcc ccggcaaacc cgttcagcgg cggcagcgca gccattgcca ttaacccgac
    22441 taacatggcg atggagataa ccggcatttt cttgccgata ccaccgagtt tttcgatatc
    22501 gcgatgaccg gtacggaacc agacgctccc cgctccgagg aacagtacgc ttttgaacag
    22561 gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca gtgccggttg
    22621 ttcgagcgcg atacccgtta cgccagcgcc cagccccagc agaataatgc cgatattttc
    22681 cagggtgtgg taagccagca ggcgctggat gttgtgttcc atcaacgcat acagaccacc
    22741 gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt
    22801 accgccaagc agtgacaggg ttaacatgcc cagcaggcca attttcatga ctaccgtaga
    22861 aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag
    22921 cggaataatc ccggccagca ggccaaagcc aatcacaccg agcagccaga tatcggaacc
    22981 gagcggcagc tgttgcatac gcatatccag caggcgcaga tccagcgtgc cataacgctg
    23041 ccacagcagc cagcaagcaa tcgccagcag cagagtgcca agacgcccca gcgcaaacca
    23101 cagtttgccc tctttgctgt tgctggtaag gaacaccgca cacaaggcca tgacttcagc
    23161 cattaccacg aacatgccga ggttgctggc gatgacggcg cagacggcgg cagccatcaa
    23221 catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta
    23281 gaggctgaca aacagaccgc acagaccgag cgtaatcagc caaatcgcgt taagcggaga
    23341 gatttgcaca tcgtagctta ccagcgacag cgcaccgctc acgccaaccg cgccagtcag
    23401 cacagtaaag cccgcggctg ccgtatacag gctaccaacc gcgccgccaa ttccggctat
    23461 ccagccgctt agcgcctttt gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa
    23521 ccacgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    23581 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    23641 ctaacgcggg cgatatcggt gttatccacc agatgcaggg ctttggtcgg gcacatccgc
    23701 acgcaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    23761 atacccggca cccagtcaag cagcgtactg atacgcgccg gagcaggcgg tgccggtggc
    23821 gctttcgggg tattggcatt tgcaggaata tccagcggac ggctgccgga aaattcaatt
    23881 gcgccaaacg ggcaggcgat gccgcacagc ttgcagctta cgcacaggct ttcattcaac
    23941 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    24001 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattcagcat cactctcagg
    24061 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg
    24121 tggcagccga tacagagcgt ggagtcagca attacaaaac gattcaccag gcattcctca
    24181 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    24241 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    24301 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    24361 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    24421 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    24481 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    24541 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    24601 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    24661 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    24721 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttacccgttt aacagagatg
    24781 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    24841 ataaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    24901 cgcagggata atcaacactg gcacaattat tgcttgtagc tggcaatagt taatgggagg
    24961 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    25021 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    25081 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    25141 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    25201 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    25261 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    25321 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    25381 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgaaga tgaaaatcac
    25441 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    25501 tcatggcgaa gcgggcactc acgcgccggg catgagccag cgtcggatgc tggaagtcga
    25561 aattgacgtg ctggacaaaa ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    25621 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    25681 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcagttattg aaggcgatca
    25741 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc acaccagcga ttcaggtgaa
    25801 cactggtaaa ggctgccacc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    25861 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    25921 cttcgatctc ggtgaaaaac acaaagtagc ggtcctttcc gttaccgaag gtgaagacaa
    25981 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    26041 ccttttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgcac gcgaagtcaa
    26101 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    26161 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    26221 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    26281 ggcagctgcg ataaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    26341 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    26401 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    26461 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    26521 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    26581 gtcataccca cgctatcttt aaattcggcc tcgaccagtt actgccggaa aacgttgagt
    26641 ttatccacgg tccgggttgt ccggtgtgtg tactgccgat gggtagaatc gacacctgcg
    26701 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    26761 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    26821 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgtaaa gtcgtattct
    26881 tcggcttagg ttttgaaacc accatgccga ccaccgccat caccctgcaa caggcgaaag
    26941 cccgcgatgt gcagaatttt tacttcttct gccagcatat tacgcttatc ccgacgctgc
    27001 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    27061 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    27121 tggtcgctgg tttcgaaccc cttgatctac tacaaggcgt ggtcatgctg gtggagcaga
    27181 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg agtggtaccg gatgccggta
    27241 acctgctggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    27301 gcggcttagg cgtgattgaa tcttctggcg tacacctgac gccggattat caacgattcg
    27361 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcacgtt
    27421 gtggtgaagt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    27481 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    27541 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    27601 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    27661 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    27721 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    27781 taatatcggc aagctggcga tttgcggcac tgcgaatgac gttgcggtca gtggcgctat
    27841 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    27901 agccgtagtg accagcatgg cagaaaccgc gcgcacggca ggcattgcca tcgttactgg
    27961 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    28021 gggcgcaatt ccggcgaata ttcattgggg cgcacagacg ctaaccgcag gcgatgtgtt
    28081 gctggtgagc ggcacgctcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    28141 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    28201 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaatgcggt
    28261 ggttcatgag ttcgcggcag cctgcggttg cggtattgaa atttctgaat cagcgctgcc
    28321 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    28381 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggctgc
    28441 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    28501 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    28561 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    28621 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    28681 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    28741 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    28801 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca
    28861 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    28921 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    28981 ccgcaggtgg gctataccca aaatttggtc actattgcct gatgccactg gcggcagaag
    29041 ggcatatttt tggtggctgt gaatttattc gttatgacga tcgcccctgg agcgaaaaag
    29101 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    29161 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    29221 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    29281 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca
    29341 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    29401 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    29461 aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt
    29521 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    29581 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    29641 atctgaattt actgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    29701 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    29761 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    29821 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    29881 gtgaaactgg cacgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    29941 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    30001 atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt
    30061 ttgaactggc ggataaaagc tccctgttcc tcgacgaagt gggcgatatg ccactggagt
    30121 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    30181 aaatcattca gacggacgtg cgtctaatcg ccgcgactaa ccgcgatctg aaaaaaatgg
    30241 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    30301 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    30361 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    30421 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcgg
    30481 tattgctaac acgcggtaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac
    30541 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    30601 ttgttcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac
    30661 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    30721 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    30781 ggttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc atcgatgaga
    30841 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    30901 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    30961 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    31021 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    31081 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccacctcat
    31141 taagatgtat ttatattaca tcttaatctt aaagggcact atgactctaa agaagaaagg
    31201 ttcgccaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa
    31261 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    31321 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    31381 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    31441 acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt
    31501 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    31561 ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg
    31621 attccctacc atgcggtgga aaactatctc gccaaactgg tgaatcaggg agagtccgtt
    31681 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    31741 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac
    31801 aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    31861 agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg
    31921 caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt
    31981 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    32041 cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg
    32101 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    32161 accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat
    32221 gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg
    32281 ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg
    32341 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    32401 ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt
    32461 attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac
    32521 gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta
    32581 caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    32641 atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    32701 gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg
    32761 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg
    32821 cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg
    32881 cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa
    32941 gataaagttc tcacctcaaa aggcaaagca ctggctctgg aaaaacagct ttatgaagaa
    33001 ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg
    33061 gaactcgacg tgctggtgaa cctggcggaa cgggcctata ccctgaacta cacctgcccg
    33121 acctttattg ataaaccggg cattcgcatt accgaaggcc gccatccggt ggttgaacag
    33181 gtgctgaatg agccgtttat cgccaacccg ctgaatctgt cgccgcagcg tcgcatgttg
    33241 atcatcaccg gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcgttgatt
    33301 gcgctaatgg cctatatcgg cagctacgta ccggcgcaaa aagtcgagat tggcccgatt
    33361 gaccgtatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ttcgaccttt
    33421 atggtggaga tgaccgaaac cgccaatatt ttacataatg ccaccgaata tagtctggta
    33481 ttgatggatg agatcgggcg tggaacgtcc acttacgatg gtctgtcgct ggcgtgggcg
    33541 tgcgcggaaa atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc
    33601 gagctgacac agttaccgga gaaaatggaa ggcgtcgcca acgtgcatct cgatgcactg
    33661 gagcacggcg acaccattgc ctttatgcac agcgtgcagg atggcgcggc gagcaaaagc
    33721 tacggcctgg cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg
    33781 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgctacgca agtggatggt
    33841 acgcaaatgt ctttgctgtc cgtaccagaa gaaacttcgc ctgcggtcga ggcactggaa
    33901 aacctggacc cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag
    33961 agtctggtgt aataaaaatt cccgatagtc ttttgctatc gggaatatta acgacaactg
    34021 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg taaaaccatg
    34081 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    34141 gatattcatg gtgaatatca gttattacaa tcccgcttac atcaactctc ttttttcccc
    34201 gaaaccgact tacttatttc tgtcggcgat aatattgatc gtgggccgga gagtcttgac
    34261 gtcctgcgcc tgctaaacca gccctggttt atctccgtta aaggcaacca cgaagcaatg
    34321 gcgctggatg cgttcgcgac cagcgatggc aatatgtggc ttgccagcgg tggtgactgg
    34381 tttttcgatt taaatgattc agagcaaaaa gaagctacag atctgttgct gaaattccat
    34441 caccttccac atattattga aatcactaac gacaacataa aatatgtcat cgcacatgca
    34501 gattatccgg ggagtgaata tctctttggt aaagaaatag cggagagcga attactctgg
    34561 cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa cggcgctgat
    34621 tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa ccagatttat
    34681 attgataccg gatcgccgaa aagtgggcgg ctgtcatttt ataaaataag gtagtctcat
    34741 gcttcttctg tgaagcatga gtaaccagat gttattgcag gccattattc atttttcgct
    34801 accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg caaaccgtcg
    34861 tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca
    34921 tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc
    34981 accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga cgatgcagaa
    35041 ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc cccgacacag
    35101 gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg
    35161 agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc catgtgaatc
    35221 agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca acttcccggc
    35281 tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt
    35341 tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc tccggacacc
    35401 agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac
    35461 acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc taccatctca
    35521 aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac
    35581 attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa
    35641 taacacacaa tgttaattta tgtgaatcag ctcaccatat cgttatcttc cagcgcttat
    35701 attcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat
    35761 ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat
    35821 gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt
    35881 tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg
    35941 acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa acaggtgctg tttggtgaaa
    36001 caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcta
    36061 gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa atgctggatg
    36121 cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc atggcctccg
    36181 gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc ggaaaagttt
    36241 atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac cagttgttag
    36301 cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt gcggggatcc
    36361 cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg atgttcgaaa
    36421 accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc gatatttttg
    36481 ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg ctgccattgg
    36541 cctcaacagc attgaatatg ttcaccagcg ccagtaacgc aggttacggg aaagaagacg
    36601 atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa tcatgatcaa
    36661 gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt ttctggtgga
    36721 aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat
    36781 cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca
    36841 atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagatct atttcaaata
    36901 ctgctctact ttcgacagta cggcgaaagg taatatcggc ccggttaccg atgcattaat
    36961 ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgccggtca acggacgtac
    37021 ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca
    37081 tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag cgcaatccac
    37141 agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggtgtggatg ccgttcgtca
    37201 agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg cgctgaccga
    37261 acaccatctg gaaattcagg gagaagcctt gcgcgatgcc ccactggtaa cgggcggttc
    37321 tggtctggcg attggcctgg cccggcagtg ggcgcaagaa aacggtaacc aggctcgcga
    37381 agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct ctcaaatgac
    37441 caaccgccag gtggcacatt accgtcaaat tgcaccagcc cgtgaagttg atgtggcacg
    37501 ctgcctctca accgaaactc tggccgctta tgcacacgaa ctggcagagt gggttctggg
    37561 ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg cattggcagc
    37621 aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaactc tattttctca
    37681 actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag gcggtgagac
    37741 ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc caaccatttc
    37801 ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc ttaaatctgg
    37861 caacttcggt gatgaagctt ttttttcacg agcccaaaga gagtttttat catgagcgat
    37921 ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt
    37981 cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat
    38041 ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg gctttccaaa
    38101 gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat
    38161 ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca cagcacatgg
    38221 tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg tccgttcaca
    38281 ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg accgggcgat
    38341 aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca
    38401 aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag
    38461 ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg ttatctgacc
    38521 gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag ctaatttatc
    38581 catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa aagccgggtt
    38641 cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc aaaagcaact
    38701 ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata ttaatgcggg
    38761 ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag acatcgacct
    38821 ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg caggcgtcgt
    38881 gcccgctggc gaagacgctg aacggtaccg ggcagtattt atcgataatc tccgctacgc
    38941 tgccgaccgc tttgcgctac acggcaagcg aatattagtt gaagcactca gccccgacgt
    39001 taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg aagaggttgc
    39061 gcgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag atggcaacct
    39121 gacgcattta atccgcgact acgctgggaa atatgcgcac gtacaaattg ccggactacc
    39181 tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc gcctgttcga
    39241 tgaggtggga tatcagggct ggatcggttg tgaatataaa cctcgtggcc tcaccgaaga
    39301 agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta caacgtaccc
    39361 atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca gggtttccca
    39421 tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg ttgctgctgg
    39481 tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg ttagtcgcac
    39541 ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg atgggcggtg
    39601 tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt atgatcgaac
    39661 actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt gacaaacgaa
    39721 ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc tttgatgtcg
    39781 gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa atatcgccac
    39841 tcaaatttgg cctgcctgtc gctggaatca tgctaactgt tcatgtggcg gtaccgccgc
    39901 atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg ctaaccatca
    39961 tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg aaaataatca
    40021 atacgcgcca atatgcgatg tcggtggaag tactggaaca gatgcaactg gctccggcca
    40081 gtgaggaagg cgcaacaaaa ttaagcgata aaataaatcc accgggcgtc gcgctggtca
    40141 cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc gcaacactga
    40201 tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca atggtagctc
    40261 taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc tggagcttac
    40321 aacacaccag cgacattatg ggctcagccc ttcccactgc ggcggtagtg attttggtta
    40381 ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc aaagcccttg
    40441 ccaatatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt atttcactgg
    40501 ctctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc gggttactct
    40561 cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg gcagcctgct
    40621 ttggtgggct tggcgcgtca catattaatg actcagggtt ctggattgtg accaaatatc
    40681 tggggttgtc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc attctcggtt
    40741 ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa taaaaaggtc
    40801 cgatgggcat cggacctttt atttaacgct taccttccac cagcagcggc gggatgctcg
    40861 gcaccattgg cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg ggataatgtt
    40921 cgcggctggt acggcgcagc ggttcggtat cgcgccaggt ataaaggcaa tgctggcact
    40981 gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc tgttcatcgg
    41041 cacaacgtgg acaaatcatc ttcttctcct tatttacgtg cagccagcat agcggtcagt
    41101 ttttcagccc aggctttggt ttccggtaaa tccaccaccg gttggctgta gtgaccacgg
    41161 ttgtccgggg cgacaggcgt agtggcgtcg ataatcagct tgtcggtgat ccccgcaggg
    41221 cttgagcctg gatcgagttc cagcacggac atattcggca actgcaccaa atcccctgcc
    41281 gggttcactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg gtcaacgtct
    41341 tcatcgacca taatcaccat cttcacgtag cccagaccat gcggcgtggt cattgcgcgc
    41401 aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat cgccagcagg
    41461 ccatgggtgt acatggcgtt taccgcctgc acttccggga actcggcttt cagctgctga
    41521 tacagcggca cgcaggtggc tggccccatc aggtagtcga tttcggtcca cggcataccg
    41581 aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat gcgcaccacg
    41641 gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc ttcgatttcg
    41701 cgtttacggc tttcgataac cccttcgagg atcacttctg aaccccacgg cacatcaaaa
    41761 ccggtcagcg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc tgccatttca
    41821 tactcagact gatcgtattt cagcggcgtg gcccccatca gcgtgatgat cggatcgtta
    41881 ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt ctgctttatg cagatgcagg
    41941 gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt cacttccatg
    42001 cggtagatgc cgacgttctg cttgccgaag ttatctgggt cgagcggatc gcgggaaacc
    42061 acgcaggctt tgtcgagata gaaaccgcca tcgccatcgt ttaaacgaaa cagcggcagg
    42121 atatcgaaca ggttaatctc ttcgccatcg acggtgttct gcgcccaggc tggattggcg
    42181 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
    42241 ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca ggaaccgatg
    42301 gtattcatcg tcacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
    42361 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcagcgttc
    42421 acctcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
    42481 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
    42541 gccagcgcct ggcatgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
    42601 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
    42661 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
    42721 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
    42781 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
    42841 taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
    42901 ggttatggct gaagtctgcg agggcagcaa catcgtgagc gctgtaaggc gtttctagtt
    42961 caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatatccc
    43021 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
    43081 tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt aacatactcc
    43141 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
    43201 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttc
    43261 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
    43321 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tatcgaacag
    43381 gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat gttggcaaga
    43441 atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg gcgacgcttt
    43501 gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat tggtgacagc
    43561 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagctg
    43621 gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta tcgcgtctta
    43681 tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc tcgcttgaga
    43741 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
    43801 cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
    43861 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
    43921 accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca gccatttgac
    43981 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
    44041 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
    44101 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
    44161 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
    44221 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
    44281 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
    44341 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat
    44401 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
    44461 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
    44521 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
    44581 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
    44641 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
    44701 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
    44761 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
    44821 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
    44881 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
    44941 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
    45001 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc ggcataaaca
    45061 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
    45121 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
    45181 caggtggaga taggcgtact ggttgatgta ctgctgacag tcggctcggt tgtgcttgct
    45241 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt cggcaacatt
    45301 ttgttagcac tctgttcacc cgaagactca gaatacgtaa ttgtcggttg cgacgcaaca
    45361 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc atcggcctgg
    45421 gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg taaggtctga
    45481 ccgacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
    45541 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
    45601 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
    45661 tccatctgta ccggctgctg agctactggc tgtactggct ggatttgcgg ttgttgagta
    45721 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgggctgt agacgtcgtc
    45781 cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg cgcattgcca
    45841 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
    45901 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
    45961 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccctac
    46021 tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc aggaaaaatc
    46081 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
    46141 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
    46201 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
    46261 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc gtgcctgcca
    46321 accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
    46381 gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat gctggacaag
    46441 atgcgccagg attgccgttt gatatcccga accggtgcca atttccagca cccgcgactg
    46501 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg
    46561 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
    46621 gaatttttca cgcggcacgg cagcaagtgc attcagcacc tgctcatcct gaataccttg
    46681 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
    46741 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
    46801 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
    46861 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
    46921 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
    46981 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg caacggctct
    47041 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
    47101 ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
    47161 accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca
    47221 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca atcggtcggg
    47281 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
    47341 agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
    47401 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
    47461 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt
    47521 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccaatt
    47581 ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcgcg
    47641 caccagtaaa gcttgtaatt cagtttctgc ggcaacagct gcttgttcga atgccagcgc
    47701 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
    47761 atcgttaacg cgacgctgta attccgccag ttcttcggtg gttgcgacaa accagctacc
    47821 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
    47881 gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg
    47941 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgctgcgccc cctgcaagtt
    48001 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat
    48061 atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
    48121 ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
    48181 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg gcacgcgagc
    48241 gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
    48301 ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
    48361 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
    48421 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
    48481 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
    48541 tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt agtggctttc
    48601 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    48661 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gcccagggta
    48721 taccccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct gtcggcacct
    48781 ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc cgccgcgcca
    48841 agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcaatcct
    48901 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcatgt
    48961 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    49021 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcacggcctt
    49081 cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatagtcg
    49141 cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    49201 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    49261 gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc gtgcggctgg
    49321 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga cgagcggcgt
    49381 catgcaccaa tacccactgc gcgtcgccag cggctttcag acctgccagc acggaatcgg
    49441 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaatttgtg
    49501 caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg ggatgcgcca
    49561 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    49621 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    49681 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    49741 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    49801 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    49861 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca
    49921 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    49981 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    50041 aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    50101 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    50161 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga aagtacaaat
    50221 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    50281 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    50341 gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    50401 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    50461 gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    50521 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcatggaaa
    50581 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    50641 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggcgcttc
    50701 gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt tcttatataa
    50761 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcgataaa
    50821 gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    50881 tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac cgacgcgacg
    50941 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    51001 attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    51061 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    51121 atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc agacgacgtt
    51181 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgcggaaag
    51241 tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga tggcgcagca
    51301 gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt gctcaggcga
    51361 tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa caccagcggc
    51421 tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc aacttcacgc
    51481 tgggtaaggt tattaatatc aacctgatag cgaatgccat caatacgcgc gcgcgtcttc
    51541 ttaccggcaa ttttgatgtc gtaactctgg cccggggaaa gcggctgttc cgccatccat
    51601 accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc cagcagcaga
    51661 tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    51721 gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc cacaccagag
    51781 ggcagcactt tgacacgttg cccgacttcc acgcgaccgg atgccagcgt tccggcgtag
    51841 ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat tggctgagca
    51901 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg accgctgtac
    51961 cacggcatac tttcactttg cgaagccacg ttgtcgcctt ccagcgcaga gagcggcaca
    52021 aagcgaatat ccagattgcc aggtaactgt tcggcgaaag tcaggtagtc ttcacgaatg
    52081 cgggtgaatg tctcttcact gtaatccacc agatccattt tgttgatcgc tacgaccaga
    52141 tgtttgatcc ccagcagcgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    52201 ttacgggcat cgatcagtaa gatcgccagc tcacacgttg aggcgccggt cgccatattg
    52261 cgggtatact gctcgtgccc gggggtgtcg gcgataataa atttacgctt ctcggtagag
    52321 aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg caggccgtcc
    52381 accagcagag ccagatccag tttttcgccc tgggtgccgt gacgcttact gtcgttatgc
    52441 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg accaatcagg
    52501 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    52561 tgcgcaatca tccaggcttc gacgccgcct tcattagcga tttgttgtgc aagtgcggtg
    52621 ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct ggtcgcggtc
    52681 aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga tgatctccgg
    52741 cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    52801 acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    52861 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat acagcggcac
    52921 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    52981 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    53041 cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt agattcgctc
    53101 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    53161 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    53221 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagctc
    53281 gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga atttccagcc
    53341 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    53401 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    53461 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    53521 tcgtatttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    53581 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    53641 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    53701 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    53761 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    53821 tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    53881 ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    53941 ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    54001 gtgatattcg gacatttaat agtcggtata tttataccgc cagcgataat cgtaagagct
    54061 ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagca ccgcagcaga
    54121 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gccgatgcca
    54181 atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta ggtgtcatgc
    54241 tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    54301 ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    54361 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    54421 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    54481 cactggcaat tgcgcgcagt cagggaatag ccgcgacgac caatcctggt ttgaataaaa
    54541 actatccgaa aggcaccggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    54601 ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt tatcagcaac
    54661 gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtcagg tttgataatc
    54721 aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt gacgttatgc
    54781 ggataatgct acctctggtg aaagagttgg cgaaggcgtc ttgatgggtt ggaaaatggg
    54841 agctgggagt tctaccgcag gggcggggga actccaagtg atatccatca tcgcatccag
    54901 tgcgcccggt ttatccccgc tggcgcgggg aacaccaacg aaattgagag caaaggtcgc
    54961 tacgcagcgg tttatccccg ctggcgcggg gaacacttca tgaagcacgc tggaatttat
    55021 atttgccccg gtttatcccc gctggcgcgg ggaacacttc atgaagcacg ctggaattta
    55081 tatttgcccc ggtttatccc cgctggcgcg gggaacacaa aaaatgatgc tgtatttcac
    55141 tattgggttc cggtttatcc ccgctggcgc ggggaacact gaccgatttt attgatcgcc
    55201 cgatagacct gcggtttatc cccgctggcg cggggaacac ccagattccg ctttctctac
    55261 cttctctttt tccggtttat ccccgctggc gcggggaaca cgggttgtgc gccctggtgg
    55321 gcgaaaacgg tggcggttta tccccgctgg cgcggggaac acattttcct gatcgagttt
    55381 tgagaatttt gtcgcggttt atccccgctg gcgcggggaa cacactaagc atacatatct
    55441 gtttttaaac aaatttattc cacatcaaca atctaccaac taaattcaaa catttcctta
    55501 tttttaaaga acacataacc tattgattat caacaggaag aaaagaaacc aaacgtaacc
    55561 catccaaatc caccggaata cgtctgtttt ctccccaggt ctgaaattca aaacccgatt
    55621 cggtattggt cgcccaggcc atcaccacat ttccgcaacc agccagttgg gtaatttgtt
    55681 gccagatcat ctcccgaata cgttttgatg tatcaccaac atacacaccg gcacgcactt
    55741 ccagtagcca gattgcgagc cgtccacgta agcgcggagg gacattttct gtaacaacca
    55801 cgaccatgct catccgccgc gcccccggtg accactatca cccagcgttt caggttcagg
    55861 gatggcaggc ggtaacatat ccggcgcggg ttgtggtggt tcaatttcac ctgcagcaag
    55921 gacttcctca attaacggta ttaatttgcc cgttaactta gtgctacgga aaatatcgcg
    55981 acaggctaat ctgacttctt tatcaggttc tgcgggttgc ctcgctgcta tttcaaatgc
    56041 ctttggcaca accgaatcaa atttaatgat atcggctatg tcataaacaa atgaaagcgg
    56101 tttgccacta tgaataaatc caatagtggg cgcatatccc gcggctaata ctgccgcttc
    56161 agaaataccg tacagacatg atgtggcagc actgatgcag cgattcacaa catcgccttt
    56221 ttcccagtct ttaggatcgt atttgcgacc attccatttc acaccatatt gtttcgccag
    56281 taatgcatag gtctggcgaa cgcgggatcc ctcaattccc cgtagctgat ccactgaacg
    56341 gcgagctggc ggtggctcac gaaaacgtaa ttcatacatt ttgcgcacca ccttcaggcg
    56401 tagatcttcc gttaaagcca gctttgcctg gtagagtaat ttatctgccc gcgcccctcc
    56461 gggttgtccg gaagagtaaa cgcgaacgcc cgcttcaccg acccagacca gcagtgttcc
    56521 caccgtggcg gccagatgca ccgccgcgtg ggaaactctc gttcccggtt cgagcataat
    56581 gcaggcgacc gatcccaccg gaatgtgcgt gcggatcccg gttttgtcga tcagcacgaa
    56641 agcgccgtcc agtacgtcga tttgaccgta ctggaggaag atcatagagg tgcgatcttt
    56701 taacgggatc ggactcagtg gtacaaacgt cacgcctctg ctccgggttt gatcagcatc
    56761 agaccacagc cgaacgcgcg gcttttgcca tacccctgac tgagacgttg cagaaataac
    56821 ccgggatcgg tgaccgtaag catccccgta taatccacgc tactgaactg gatcagttgc
    56881 cgggagtttt cccgccgcag ttgctgttgt cggtaggcat caaccgaggt atccagcagt
    56941 gtaaaaccgc ttctctcccc ctgcgctgcc agccagtcca gcgccgcctg ttgttgatgc
    57001 aaccagacat cacgcccttc cgcctgtccc ctcacctgcc gtttcgcctc catcagcaga
    57061 tcgtggcgct tgcccgcttt acaaattgtg ggattcgccc gcaggttgaa acaaagggat
    57121 tgtccggtac gcagctccgg agcaaatgac cggcattcga tagtgaaagt ttcactttcc
    57181 gccgggcgtt cctgcgagag gacaaaaaag cgaaacgcac cctgaagttc ctcccgacga
    57241 taaagaaatt gcctttcttt gccgccaggg aagagatccc acagccactg atgcatcaca
    57301 tattcaccgc gatccaccag atgcagcaac tgcgcaggca aaagctgagc ggtatgtaag
    57361 gttattcttg agaggtacat ggttcctcct tgctgagcca cggcccctga ttgacggtgc
    57421 gttccccaaa cagccactgc tggcgattta aaggaacatc ccggcggcgt aatattttgc
    57481 tcacgaccag gccgtcgtgc tccccttccc accagcattc atcctgaagt ttcgggagtg
    57541 agactttcag gtcgtggaaa tgatcctgat actgttggta tgcgttacgt aaaacatcag
    57601 acgcgttgcc ttcgagcagt aacggcgcaa gtggtaatgc cagcggatga ctttttcgcc
    57661 ccagataaag aggaaaaact ggatggcgta aaccgccctg caactgttcg aggctgtaag
    57721 gcgcatcggc tgttgttgcc accgccacca tccaccaggc gtcggtgtag tagtcgcgcc
    57781 gggagataat cgcgctcaga agatcagggt cgctcaactc ttcgcgacgg ctgaaataac
    57841 gcgctttacg cacctctttt ggcatctgga tcgtgtgata atcccgtgcc cagcgcgggt
    57901 tacggctggc gcaaaccacc agtgaatagt ggcgattaaa cgcgtttaag cgttcggtat
    57961 catcacgccg aatccctacc ccggcagcca gcagacccag caatgctgag cgcgaaggca
    58021 gttcatgggt atgacgcact tcgccggggg catcgacgcc ccaggatgcc attggcccat
    58081 gaagctgaaa aatcagatat tggctcataa gccgcccctt acgcgcagat aaagtccagc
    58141 acgtccttca tgcttccctg tttgttcatc acgtcaaaac ttgcgcattc ggtcttctgt
    58201 tcgtagaccg tattcatatt ttcgcgaagc gttgtaatac gctgcaccgc cacgtctaac
    58261 tgccgggtgc cattaatggg ttcatagaag gccgcagcca gagaacgcgg ttgatcggtg
    58321 cctttttccg ccagcgccca ggaagcatag gcacggctgg caaagctatt ttgtttaccg
    58381 gttggggaaa ctttaagcgc ggcttccgta aaagcgcgta tggtctgatt agctaacgct
    58441 tcgtcaccgc cgaggttttc taccagcaga tctttatcaa tgcagatata ggtgtagaaa
    58501 agtgctgaac cgaatccggt ttctccgaga tgcccggcac cggcatcttc agaggcctgg
    58561 cgcaaatcat caacggcggt gaaaaaatca tcttcgacaa tcgtttcact gacaccaaac
    58621 gcatgcgcga cctggcaggc ggcttcaaca ttaaactcag gtttattcgc cagcatacga
    58681 ccaaacatgg cgatatctac cgccatacgg tctttgcgta acagactgag atcttcctct
    58741 tttggcgcgc gcttttcttc tgccagttga tgggccagcg cttttacggc gtcaaattct
    58801 gccgggctga tatggactaa ttgttcagtt tcggcgttag tgagcggatc ttttggtttt
    58861 ttgtcgtttt tagctttccc aagataatct gcaatttttg ccgcccattc gatggctttt
    58921 ttatcttcga ttcctttctc aatcaggata gttgccgcct cacgcgcgat acgcccactg
    58981 cgaataccaa tatggcccgc cagcgcctgt tcaaaaagtg cagaagtgcg ccacgcacgt
    59041 ttcagacttt gcgaggaaac gcgcagacgc gttgctccac ccaacaccac ggtttttggc
    59101 gcaccggtgt catcacggtt aaggttggca gcagggtaag cggttaacaa atgaagctga
    59161 ataaatgtcg tcatgagata gtcctttatt aattttgttc gttatcggcg tcaccggcct
    59221 ggtaatattc cagcgcccag cgaatacgga taaattcggt tggcctctgc tggcggcggt
    59281 gatgattgag caggtcgtcg ctctcctggc accagcggaa aatatcgtct gccaaagaga
    59341 taagattgac ggaaccgttt aataatttga ccgcgcgacg taactgacgc agtaaatcat
    59401 ccggcgtttt tactgccgac aggcaagtaa aacgcccctc tgacattacc gccgccagct
    59461 gtgcggcaaa aggctgccgt tcatcaatgg ctttaacatt ggcggcaacg gcggcgacca
    59521 gtgccagtgc ggtaaaacgc cactcttgtg cttcaatttt ccacaaagtg tgggtctgca
    59581 tcagcagaga acgaaaccct tctgacaagc aaacatcatt aaccgttgtg ctacgccgca
    59641 gactggcacg attgccgcgt ttagcctgca actcttcgtg ccatttacgc agcgtggctt
    59701 tgtgcccgtc ttttacaata ctcattcagc agcctcctgc tttttctccc tggcggcttt
    59761 agcactttgc ttctccgccg atgttgtaaa atattttttg cgcgcggtca tgacgcgttt
    59821 taaatcaacg ggctcataag gattggtgaa tacacgctcg tcaaaatcct gacgcgcgaa
    59881 taaccaaatc tccttattcc atttgctgag taatccatcc gcatcctgac cttcttcaat
    59941 ttggcgcact aacctcagga agcgatgctg agttttgttc cagaagtcga tatccacaaa
    60001 actgaaatca ccccgtgcac cttttggatc ggagaaccat gcttctttca atgcactccg
    60061 taacagactt agaatgcgtg aagccgtttg cgcagccagc cgcagtttcg gtatctggcc
    60121 ttctttttta ttgagcagca gcgggaaacg gtgttcgtac caacagcggg ctttcatgtt
    60181 gtcgaaatca tagccaaatc cccataggcc tacttttgcc tgcttgagac tgctggcatt
    60241 aaagagtttc accaccagag cgggaagttc cgtattgttt tctgacttac ccgtttcgat
    60301 aaggcctaac cagtcgcgcc aaattaaacc gcctggttgt ggtttaactg agtaaaactc
    60361 accaccctct ttaagtggta cacggtaagg cgttaatgga tgctgccaca tggcataatt
    60421 cgcaccgtaa tttttggtcg tcatcaggct cagaagcgcg tcgtcctgct caccgcaaat
    60481 atcgcagttg ccgactgtcg tggtattaaa atcaatacga atacgccgcg gcattcccca
    60541 gtacgcctgg agtttattga cctgatcatc ggttaccacc gcaccggcca gttcgctggt
    60601 acgcgtcggg ccaagccagg ggaaaaccag atcgtcaaat tttttgggta gcggtaagtc
    60661 ggcttcatcc tgcggcatca cgttgagcca cagtttgcgc cacaaggggg cttgttgatt
    60721 gccctgatac tcctgcaatt caatcagagt cgtcatcggc ccaccgccgc gtataccggt
    60781 gcgatagcct ttgccacctg acggcgcatt taactgtagg gagaacagag ctaacgcaga
    60841 acaatgagag catacgtgtt cagtcacgcc acgcttaata aagtggtctt tattaaactt
    60901 cgttgtttga gcgccgggaa tctcaggcag tagcgaagcg acctgaactt tatcgcccat
    60961 gagcacctcg aaatcctgca taaatgaagg tgaatctggg ccaaactgga aagcgtgttc
    61021 taatgacagc aatgcttccc gtagcttttc agcttccagc ccgtcttccc agatatcatc
    61081 ccaacgacga taatcttttg gcgcgaaact gctttgtagt aaccccagca aaaactgcca
    61141 tgccgccccc tggagatctg cccgcggcgc agcgatatcg acaacatttt catccgccag
    61201 atcgactggc gccagtttgc ctgtcgttcc gtctttaaaa cgaacgggca accacggggt
    61261 tgtcagaagt gaaaacgagt tcatacgcat cctctcgtaa aaaagaccat ccctgatcgg
    61321 ttatccatca ttccatcttt ttaggtgtat caactattca gagagctcta tcgcaggcat
    61381 ctcaaccaac cttgccaatc aatccctccg tagccgaata cccacaactt tcgttatcgg
    61441 tgactaaaat cacgtttgcc atctctggat cttgccgctg ttcaatgcac cactgcctga
    61501 acgcttcccc ttccagtaaa gaaaactcat cgcgatgttt tttccaccag cttcgacgca
    61561 ctctgacaac actcatttcc catgcgtgag cgccagtggt ataaggcttc accacaccgt
    61621 caatacaggt agccagccac agggaaacag attcctcagc cagacgtgtc gacagttttt
    61681 cagggaggta atcgttgata ttggcggcat agccgggcct gaagttgagt acaaactttt
    61741 tagccattgc acgatcgcag taatatttgc ccacttgctc ctgctcggag cgagcaaacc
    61801 cttccggcat caccacgtcc tcaccgtaga ctgattcaat aagaagccgg gctgagtgtg
    61861 gcatttgaat agcgccttgc tgacgcagta cacgctgcgt cagccagatt cgtccatgat
    61921 cgggatagat atatgcactg ttccgcatgg cactgccaaa ccattcttca tcaggtgaat
    61981 cgtcccagac gggggccaga atcagcaatt caggagggga acgctcgtct tttccatcac
    62041 gctttaactg accattaata tcgcggatat gccgctgtaa tcgccccgct cgctggatca
    62101 gcaaatcaat gggggcaagg tcggaaatca tttcgtccag gtcgcaatca acgctctgct
    62161 ccagtacctg agtgctaata agggcttttc cggcacgctg tgagctacct tctttaccaa
    62221 agcgcgccag cgttttcgtt tcgattcgct ggcgatcgct aaaagcaaag cggctatgaa
    62281 agagtgaaag gctggaagtg ggaatgacgc cgcgggcaag cagctgacga tgaaccttaa
    62341 tagcgtcatc gacagaattc cggatccagg cgatgcattt tccctgactt accgccgact
    62401 cgatacgcgc aatactctct tgttcactat gaagccaacc cacgctgacg ctacgctcaa
    62461 cgtctttgcg cgtcgctacc cggtgtgagt tcacatcgga tttcgtgaca tgcgtcagcc
    62521 aggggtaatc atccttttcc aggaacggag cttcttgctg gccctctgta ccccgcgcaa
    62581 aggcagcgac gagtttgtcg cgctgctgtt gagataacgt ggcagaaagc aaaataacgc
    62641 tgtttccgcc acgcgcctgc cgctcgatca acccttcaag aatgcacgac atgtaagcgt
    62701 cacaggcgtg gatctcatca gccagcagga ttttgttact cagccccaga agccgcagat
    62761 tattatgttt gaacggcatc accgccatca tcgcctgatc cagcgtgccg acgccaattt
    62821 cagccagcaa cgccttcttg ttactgtcgg caaaccaggc cgcacatccc tgactgaacg
    62881 tttgttcatc cggttcttct gacccgacta aatcaccaga ccagagtgat tcattaaagc
    62941 ggtccattaa tgtgcgggca ctatgtgcca gcaccaggct ggggcgggat tctggcgaat
    63001 agaaagcaag ccaggttttg accagccgat cgtacatggc attggccgtt gccattgttg
    63061 gcagaccaaa aaacagcccc tgagctttcc ccgcagctat caacctgtgc gccaggataa
    63121 gcgccgcttc tgttttacct gcgccagtca catcttccag aataaataac tgcggtcctt
    63181 gctggctgat atccagatcc agcacctttt gctgtaacgg tgtcggattc tcaataaaag
    63241 gaaacagcgt attaattccg ttgaaaggcg cgacttttgc ttttagagga aagacggtta
    63301 aggcgttttg agcctgaatt aaagttttct gccagtaacc tttaatatcc attgggtgtg
    63361 cgacgcgtgg aaaaaatcgc gttgacgaac ccgtccagtc tgcgagcacg acggttgcag
    63421 agatatacca ggaaaggtgt tttataagtt caatgccctc atcatcatcc cagaatgcgg
    63481 ggatctctat gagcggaaac agtggtttga tttcaaggag aaaatctcgt gctgcagctt
    63541 tgtcttcagg cagaaaatta tccaattcat ccatacgatc agggggctga ccatgatgcc
    63601 ctgtcgtcac gggcatccac atttctatca ctcgcctaag tttgcgcgga gagagcgaag
    63661 atgaaggaag taactcctga cattcactta aataatgatt ccacaaccag tagcccagcg
    63721 ttgaatgaga gattttttcg taattttttc tggcaccttc ctgaatcttg agttcagggg
    63781 gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat atcgtgccag cacagcaacc
    63841 aggcgaaaaa ttgagccgcc tgttccttgt cagaaatccc taattgacga aaataatcag
    63901 ccagcccgaa gcaatttctt ttaaccatta aatagcccat cgcggctaca tccagcgaat
    63961 gccagcaaag aaggtgatag tcgtcgcccc cgtctttctc gccgcgtcgg gtttttcccc
    64021 agaaatcaaa aaaagtcaca atatttttat ccttcagtaa acttaaagga tatttacgca
    64081 tattttaa
//
LOCUS       DAFAVF010000008        62397 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFAVF010000008 DAFAVF010000000
VERSION     DAFAVF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.008"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(149..1447)
                     /gene="eno"
                     /locus_tag="LGK42_000493"
     CDS             complement(149..1447)
                     /gene="eno"
                     /locus_tag="LGK42_000493"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK2656782.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1535..3172)
                     /gene="pyrG"
                     /locus_tag="LGK42_000494"
     CDS             complement(1535..3172)
                     /gene="pyrG"
                     /locus_tag="LGK42_000494"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBK2656783.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3400..4191)
                     /gene="mazG"
                     /locus_tag="LGK42_000495"
     CDS             complement(3400..4191)
                     /gene="mazG"
                     /locus_tag="LGK42_000495"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBK2656784.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4262..4597)
                     /gene="mazF"
                     /locus_tag="LGK42_000496"
     CDS             complement(4262..4597)
                     /gene="mazF"
                     /locus_tag="LGK42_000496"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBK2656785.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4597..4845)
                     /gene="mazE"
                     /locus_tag="LGK42_000497"
     CDS             complement(4597..4845)
                     /gene="mazE"
                     /locus_tag="LGK42_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBK2656786.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4923..7157)
                     /locus_tag="LGK42_000498"
     CDS             complement(4923..7157)
                     /locus_tag="LGK42_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBK2656787.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7205..8506)
                     /gene="rlmD"
                     /locus_tag="LGK42_000499"
     CDS             complement(7205..8506)
                     /gene="rlmD"
                     /locus_tag="LGK42_000499"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK2656788.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8563..11319
                     /gene="barA"
                     /locus_tag="LGK42_000500"
     CDS             8563..11319
                     /gene="barA"
                     /locus_tag="LGK42_000500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBK2656789.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLQNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11551..12891)
                     /gene="gudD"
                     /locus_tag="LGK42_000501"
     CDS             complement(11551..12891)
                     /gene="gudD"
                     /locus_tag="LGK42_000501"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBK2656790.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12912..14252)
                     /locus_tag="LGK42_000502"
     CDS             complement(12912..14252)
                     /locus_tag="LGK42_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBK2656791.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIVTNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14254..15606)
                     /gene="gudP"
                     /locus_tag="LGK42_000503"
     CDS             complement(14254..15606)
                     /gene="gudP"
                     /locus_tag="LGK42_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBK2656792.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16041..16490)
                     /locus_tag="LGK42_000504"
     CDS             complement(16041..16490)
                     /locus_tag="LGK42_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK2656793.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16508..17290)
                     /gene="truC"
                     /locus_tag="LGK42_000505"
     CDS             complement(16508..17290)
                     /gene="truC"
                     /locus_tag="LGK42_000505"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBK2656794.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17290..17619)
                     /locus_tag="LGK42_000506"
     CDS             complement(17290..17619)
                     /locus_tag="LGK42_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBK2656795.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18241..18786)
                     /gene="syd"
                     /locus_tag="LGK42_000507"
     CDS             complement(18241..18786)
                     /gene="syd"
                     /locus_tag="LGK42_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBK2656796.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18854..19702
                     /gene="queF"
                     /locus_tag="LGK42_000508"
     CDS             18854..19702
                     /gene="queF"
                     /locus_tag="LGK42_000508"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBK2656797.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19814..21178
                     /gene="ppnN"
                     /locus_tag="LGK42_000509"
                     /gene_synonym="ygdH"
     CDS             19814..21178
                     /gene="ppnN"
                     /locus_tag="LGK42_000509"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBK2656798.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21735..23024
                     /gene="sdaC"
                     /locus_tag="LGK42_000510"
     CDS             21735..23024
                     /gene="sdaC"
                     /locus_tag="LGK42_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBK2656799.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23082..24449
                     /gene="sdaB"
                     /locus_tag="LGK42_000511"
     CDS             23082..24449
                     /gene="sdaB"
                     /locus_tag="LGK42_000511"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBK2656800.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24561..25316
                     /gene="xni"
                     /locus_tag="LGK42_000512"
     CDS             24561..25316
                     /gene="xni"
                     /locus_tag="LGK42_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBK2656801.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25371..26519)
                     /gene="fucO"
                     /locus_tag="LGK42_000513"
     CDS             complement(25371..26519)
                     /gene="fucO"
                     /locus_tag="LGK42_000513"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBK2656802.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26547..27194)
                     /gene="fucA"
                     /locus_tag="LGK42_000514"
     CDS             complement(26547..27194)
                     /gene="fucA"
                     /locus_tag="LGK42_000514"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBK2656803.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27741..29057
                     /gene="fucP"
                     /locus_tag="LGK42_000515"
     CDS             27741..29057
                     /gene="fucP"
                     /locus_tag="LGK42_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBK2656804.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29090..30865
                     /gene="fucI"
                     /locus_tag="LGK42_000516"
     CDS             29090..30865
                     /gene="fucI"
                     /locus_tag="LGK42_000516"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBK2656805.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30974..32392
                     /gene="fucK"
                     /locus_tag="LGK42_000517"
     CDS             30974..32392
                     /gene="fucK"
                     /locus_tag="LGK42_000517"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBK2656806.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32394..32816
                     /gene="fucU"
                     /locus_tag="LGK42_000518"
     CDS             32394..32816
                     /gene="fucU"
                     /locus_tag="LGK42_000518"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBK2656807.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32874..33605
                     /gene="fucR"
                     /locus_tag="LGK42_000519"
     CDS             32874..33605
                     /gene="fucR"
                     /locus_tag="LGK42_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBK2656808.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33649..34749)
                     /gene="rlmM"
                     /locus_tag="LGK42_000520"
     CDS             complement(33649..34749)
                     /gene="rlmM"
                     /locus_tag="LGK42_000520"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBK2656809.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34742..35137)
                     /locus_tag="LGK42_000521"
     CDS             complement(34742..35137)
                     /locus_tag="LGK42_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBK2656810.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35156..36073)
                     /gene="gcvA"
                     /locus_tag="LGK42_000522"
     CDS             complement(35156..36073)
                     /gene="gcvA"
                     /locus_tag="LGK42_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBK2656811.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36424..36651)
                     /locus_tag="LGK42_000523"
     CDS             complement(36424..36651)
                     /locus_tag="LGK42_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBK2656812.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36843..38048
                     /gene="csdA"
                     /locus_tag="LGK42_000524"
     CDS             36843..38048
                     /gene="csdA"
                     /locus_tag="LGK42_000524"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBK2656813.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38048..38491
                     /gene="csdE"
                     /locus_tag="LGK42_000525"
     CDS             38048..38491
                     /gene="csdE"
                     /locus_tag="LGK42_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBK2656814.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(38542..39348)
                     /gene="tcdA"
                     /locus_tag="LGK42_000526"
     CDS             complement(38542..39348)
                     /gene="tcdA"
                     /locus_tag="LGK42_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBK2656815.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39425..40522)
                     /gene="mltA"
                     /locus_tag="LGK42_000527"
     CDS             complement(39425..40522)
                     /gene="mltA"
                     /locus_tag="LGK42_000527"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBK2656816.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40731..40807
                     /locus_tag="LGK42_000528"
     tRNA            40731..40807
                     /locus_tag="LGK42_000528"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40765..40767,aa:Met,seq:cat)
     gene            40841..40917
                     /locus_tag="LGK42_000529"
     tRNA            40841..40917
                     /locus_tag="LGK42_000529"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40875..40877,aa:Met,seq:cat)
     gene            40951..41027
                     /locus_tag="LGK42_000530"
     tRNA            40951..41027
                     /locus_tag="LGK42_000530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40985..40987,aa:Met,seq:cat)
     gene            complement(41102..42355)
                     /gene="amiC"
                     /locus_tag="LGK42_000531"
     CDS             complement(41102..42355)
                     /gene="amiC"
                     /locus_tag="LGK42_000531"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBK2656817.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42587..43918
                     /gene="argA"
                     /locus_tag="LGK42_000532"
     CDS             42587..43918
                     /gene="argA"
                     /locus_tag="LGK42_000532"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBK2656818.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43980..45806)
                     /gene="recD"
                     /locus_tag="LGK42_000533"
     CDS             complement(43980..45806)
                     /gene="recD"
                     /locus_tag="LGK42_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBK2656819.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(45806..49348)
                     /gene="recB"
                     /locus_tag="LGK42_000534"
     CDS             complement(45806..49348)
                     /gene="recB"
                     /locus_tag="LGK42_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBK2656820.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49341..52229)
                     /gene="ptrA"
                     /locus_tag="LGK42_000535"
     CDS             complement(49341..52229)
                     /gene="ptrA"
                     /locus_tag="LGK42_000535"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBK2656821.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52405..55773)
                     /gene="recC"
                     /locus_tag="LGK42_000536"
     CDS             complement(52405..55773)
                     /gene="recC"
                     /locus_tag="LGK42_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBK2656822.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPTTGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55786..56109)
                     /locus_tag="LGK42_000537"
     CDS             complement(55786..56109)
                     /locus_tag="LGK42_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK2656823.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56094..56501)
                     /locus_tag="LGK42_000538"
     CDS             complement(56094..56501)
                     /locus_tag="LGK42_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBK2656824.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56498..57061)
                     /locus_tag="LGK42_000539"
     CDS             complement(56498..57061)
                     /locus_tag="LGK42_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBK2656825.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(57052..57522)
                     /locus_tag="LGK42_000540"
     CDS             complement(57052..57522)
                     /locus_tag="LGK42_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBK2656826.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57707..58501)
                     /gene="thyA"
                     /locus_tag="LGK42_000541"
     CDS             complement(57707..58501)
                     /gene="thyA"
                     /locus_tag="LGK42_000541"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK2656827.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58508..59383)
                     /gene="lgt"
                     /locus_tag="LGK42_000542"
     CDS             complement(58508..59383)
                     /gene="lgt"
                     /locus_tag="LGK42_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK2656828.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59534..61780)
                     /gene="ptsP"
                     /locus_tag="LGK42_000543"
     CDS             complement(59534..61780)
                     /gene="ptsP"
                     /locus_tag="LGK42_000543"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2656829.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61793..62323)
                     /gene="rppH"
                     /locus_tag="LGK42_000544"
     CDS             complement(61793..62323)
                     /gene="rppH"
                     /locus_tag="LGK42_000544"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBK2656830.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT        15077 a        16915 c        15304 g        15101 t
ORIGIN      
        1 gtaaggaata gcagaaaaat tgttcggata cttatcctgg tgggaataac atttattaat
       61 gttatcgctc tgcttactaa actcaaaatg tgaagataaa aaaacccgct tcaagaagcg
      121 ggttttttat taagacgtta agtcagtctt atgcctggcc tttgatctct ttacgaccgt
      181 tgtacggtgc tttttcgccc agagcttctt cgatacgaat cagctggttg tatttagcaa
      241 cacggtcaga acggctcata gaaccagttt tgatctggcc tgcagcagta ccaacagcca
      301 ggtcagcgat ggtagcgtct tcagtttcgc cagaacggtg agagataact gcagtgtagc
      361 cagcatcttt cgccatcttg attgcagcca gagtttcggt cagagaaccg atctggttga
      421 atttgatcag gatggagtta gcgatacctt tttcgatacc ttctttcagg atcttggtgt
      481 tggttacgaa caggtcgtca ccaaccagct ggattttgtc gcccagaact ttggtctggt
      541 atgcgaaacc gtcccagtca gattcgtcca gaccgtcttc gatagaaacg atcgggtact
      601 gtttggtcag ttcttccagg aagtgagtga attcttcaga ggtgaacgct ttgttgcctt
      661 cgccagccag aacgtattta ccatctttgt agaattcaga agctgcgcag tccatcgcca
      721 gagtgatgtc tttgcccagt tcataaccag cagctttaac agcttcagcg ataacagcca
      781 gagcttcagc gttggaaccc aggttcggcg catagccacc ttcgtcacca acagcagtgt
      841 tcatgccttt cgctttcaga acttttgcca ggtgatggaa aacttcagaa cccatgcgga
      901 tggcttcttt cacagttttc gcgccaaccg gctgaatcat gaattcctgg atatcaacgt
      961 tgttgtcagc gtgctcacca ccgttgatga tgttcatcat cggaaccggc atagagtatt
     1021 tgcccggagt accgttcagt tcagcgatgt gctcgtacag cggcatacct ttagcagctg
     1081 cagcagcttt ggcgttagcc agagatacag ccaggattgc gttcgcgccg aatttggatt
     1141 tgttttcggt gccgtccagg tcgatcatga tcttgtcaat gccagcctga tctttagcat
     1201 ctttgccaat cagcgcctga gcgatcgggc cgtttaccgc agcaacagct ttggttacgc
     1261 ctttacccag gaaacgggat ttgtcgccat cgcgcagttc cagagcttca cgggaaccag
     1321 tagaagcacc tgacggagca gctgccatac cgacgaaacc accctccaga tgtacttcgg
     1381 cttcaacagt cgggttacca cgggagtcga tgatttcacg accgatgatt tttacgattt
     1441 tggacattag gttttcctca agtcactagt taaactgaaa ctccagacaa acaacgcgta
     1501 cccagggtac gcgttgccgt tctaactttt ttacttactt cgcctgacgc ttctggaact
     1561 cgctggcggc ttttacaaag ccagcaaaca gcgggtgacc atcacgtgga gtagaagtaa
     1621 actccggatg gaactggcaa gccacgaacc acgggtgatt cggaacttcg atgatctcga
     1681 ccaactgatc atccccggaa cggcccgcaa cgcgcagacc tgcatcttca atctgtttca
     1741 acagcatgtt gttgacttcg taacggtgac gatgacgctc aacaattgtc ggcgcattgt
     1801 acagctggcg aaccaggcta tcgtcaacca actggcactg ctgtgcgccg agacgcatgg
     1861 taccgccgag atcgctcttc tcgctacgaa cttcaacgtt gccgttttca tcgcgccact
     1921 cggtaatcag cgccacaacc gggtacttac agtctggcac aaattccgta gagttggcgt
     1981 tctccatgtt ggcaacatgg cgagcgtaat caattaacgc cacctgcata cccaggcaaa
     2041 tgcccagata aggaatattg ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta
     2101 cgccacgata gccaaaaccg ccaggtacta ggattgcgtc cagaccttta aggatttcaa
     2161 cgccgcgcgt ttcaacatct tgtgaatcga ttagtttgat gttgacgctg acacgattct
     2221 tcagcccacc gtgtttcagt gcttcgatca ctgatttata agcatccggc agttcaatgt
     2281 acttgccgac cataccgatg gtgacttcac ttaccgggtt cgcttcttcg aagataacct
     2341 gttcccattc ggacagattc gcttccgggc agtttaagct gaatcgttta caaatataat
     2401 cgtccagccc ctgagatttc aacaggcccg gaattttata gatggaatcg acgtctttca
     2461 gagaaataac cgctttttcc ggaacattac agaacaatgc aatcttcgca cgttcgttcg
     2521 ccggaacagc gcgatctgaa cgacaaatca ggatgtcagg ctggataccg atggagagca
     2581 gctcttttac agagtgctga gtcggtttgg ttttgacttc accagacgct gccatgtacg
     2641 gcaccagcgt caggtgcata aacagagtgt gctcacggcc aatttcaaca gccatctggc
     2701 gaatcgcttc gaggaacggc aaggattcga tatcacctac tgtaccgccg atttctacca
     2761 gtactacgtc atgaccttcg ccaccttcca gcacgcgctc tttgattgcg ttagtgatgt
     2821 gcggaataac ctgcacggtt gcgccgaggt agtcaccgcg gcgttcttta cgcagaacgt
     2881 cagagtagat acgacccgtg gtgaagttgt tgcggcggct cattttggta cgaatgaaac
     2941 gctcgtagtg ccccaggtcc aggtcggttt cagcgccgtc ttcagtaacg aacacttccc
     3001 cgtgttggat tgggctcata gtacctggat cgacgttgat gtacggatcc agtttcatga
     3061 tggtcacatt gaggccacgg gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt
     3121 tacccagaga ggatacgacc ccgccggtca caaaaatata gttcgttgtc atgctgaacc
     3181 tgagaagtta ggttgaaaag acgatggaat aaccaggacg ggaaagcagt ataccctaac
     3241 atgacctgtg ccacaaactt tcattatccc tcctcttcgc cagcgcacta ttgaaatcag
     3301 gagtgagaaa atagcccctt tggggtaaat gtttttgacg caaatcaagc gcttgtcatt
     3361 taaaaaatga cacaaatggc gcttgaccgc gtaattccct tagagatcaa tttcctgccg
     3421 ttttacctgt tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc
     3481 acgcgcggca acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcgcttt
     3541 ttgcaatgcg atttctgctt tcgtccctaa atggcgagcc agattaaccg tggcaaacag
     3601 caggtccccc atttcctcct ccagtttagc ctggtcgaca acagcctgcc gcgcctcgta
     3661 catcacctcg tcgatctctt cgtagacttt atcaaccacc gggccaagcg tcgtccagtc
     3721 gaagccaacg ttggcgcaac gtttctggat tttttgcgcc cgcattaaag ccggtaaact
     3781 acgaggaata tcgtccagcg ccgaatgctg cgctttctgc gcgcgctctt cggttttgat
     3841 ttgctcccaa cgggcaagca cttcactact gttttcggca gaactatcag caaaaacatg
     3901 cggatggcga cgctctaatt tatcgctaat agcagcgcaa atatcattaa agtcaaagcg
     3961 cccttcttcc tgagccattt gcgcgtaaaa caccacctgg aatagcagat cgcccagttc
     4021 accgcgaaga tcgtcaaaat cttcacgggc gatggcgtcc agcacttcgt aggtttcttc
     4081 aagggtgtaa ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc
     4141 cggatcgcgc aggcgctgca taatagtgag caaacggtcg atttgattca ttgaattgtc
     4201 ctgaaaattg cgggtctgtc aggtggaaac ctgtgaccag aatagaagtg agttagtaac
     4261 actacccaat cagtacgtta attttggctt taatgagttg taattcctct ggggcaactg
     4321 ttcctttctt cgttgctcct cttgcccgcc aggcgatact ttttacctga tcagctaacg
     4381 ctacgccatc acgttcctga ccggataaaa caacttcgaa cggatatcct tttgattgcg
     4441 ttgtataagg aacacacaga cacatacctg ttttgttgtt gtacatgaaa ggactcagga
     4501 caacagctgg acgatgtcca gcttgctcgc taccttttgt cgggtcaaaa tcaacccaaa
     4561 tcagatcgcc catatcgggt acgtatcggc ttaccattac cagacttcct tatctttcgg
     4621 ctctccccag tcgatattct cgtggaggtt ttccggcgtg atgtcgttga ccagttcagc
     4681 aagcgtaaat acgggctctt tacgcactgg ctcaataatt aatttgccat ccaccaggtc
     4741 aatcttcact tcatcatcaa tattcagatt gagcgcctgc attaacgtag ccgggatccg
     4801 caccgccggt gaatttcccc aacgctttac gctactgtgg atcataaccc tttcctcaaa
     4861 ccgctatcat atgtagatac agtatatatc aatctacatt gtagatacga gcaaatttcg
     4921 gcctaactcc cgtgcaaccg acgcgcgtcg ataacatccg gcacctggtt gagtttaccc
     4981 agcacgcgcc ccagcacttg caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt
     5041 tgctgtttgg tgtcgctacg gctggcaacg ccaagcacgt tcaccttctc gttggcgaga
     5101 atggtcgtga tatcacgtaa caacccacta cgatcattag ccaccacgcg gaccaccagc
     5161 gaatatccgg cggagtagct ctcaccccat accgcgtcaa caatgcgttc tggcgcatgg
     5221 gagcgcagtt ccgccagttg ttcgcaatcg gcgcggtgta ctgaaatacc gcgcccctgg
     5281 gtaatgaagc cgacaatctc atctccagga atcggctggc agcagcgcgc gatgtggtgc
     5341 atcaggttac cgacaccttc aaccaccacg cgaccgttat ctttactgcg gttttgcggc
     5401 gtgtagcttt tttgctgaag ttgcttcagc gcggcggcgt cctgctcttc ggcactcggc
     5461 ttattaaatt gcgattgcag gaagttcacc atctgattga gacggatatc cccgccacca
     5521 atcgccgcca gcaactcgtc gacatcatta aagttgtaac gcggcagcag atgtttttct
     5581 gcttctttca ggctgatccc cagatgttcc agctcgtcgt caaggatttg ccgcccagcc
     5641 agaatgtttt tgtcacggtc ctgtttacgg aaccaggcgt gaattttcga acgcccacgg
     5701 ctggttgtga cgtaaccgag gtttgggttt aaccagtcac ggctggggtt cggctgtttc
     5761 tgggtgataa tttcaatctg gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc
     5821 ccgccaattt ttgccccgat gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag
     5881 tccagcggcg ttgatcccgc aggcaaatca acgacatcac ctttcggcgt aaagacatac
     5941 acccggtcgt caaagacctg gctgcgtact tcgtcgagca tttcgccgga atcagccatc
     6001 tcttcctgcc acgcaatcag tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt
     6061 gcgccgccag ctgccgcgcc ctctttatat ttccagtgcg cagcaacacc caactctgca
     6121 tcttcatgca tctgtttggt gcggatttgg atctcaacgg ttttgccacc tggccccaga
     6181 accacggtat gaatagactg ataaccgttt ggtttcgggt tagcgacata atcgtcaaac
     6241 tcatccggca ggtggcgata gtgagtgtgc actatcccca gtgcggcata gcaatcctgt
     6301 aaacgctcgg cgacaatacg taccgcacgc acatcaaaca gctcatcaaa ggcgaggttc
     6361 tttttctgca ttttacgcca gatgctgtag atgtgtttcg gacgaccata cacttcagct
     6421 ttaacgcctt cagctttcat ctcagcgcgc agatgaccga caaactcttc gatatagtgt
     6481 tcgcggtcga gacggcgttc atgcagcagt tttgcgatgc gtttgtattc ggtcgggtgc
     6541 agataacgga agcagtaatc ttccagctcc catttcagtt gcccaatccc aagacggttc
     6601 gccaacggcg cgtagatatt ggtgcactct tttgccgcca gtacgcgttc atcttccggc
     6661 gcatctttta cttcgcgcag atgagcaata cgctccgcca gtttgatgac cacgcagcga
     6721 aaatcatcga ccatcgccaa taacatccgg cgaacgttat cgacctgttc ggaggaaaca
     6781 gaatcagtgt gcgtcgcttt cagctggcgg atcgccgcca tatcacgcac gccgtgaata
     6841 aggttaacaa ccgacttacc gacgctctca cgcagcacat cttcgctaac tacgttggca
     6901 tcagccagag ggaacagcag cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag
     6961 aggatctcca ccatctcaac accacgccac aataacagac tggcatccgg atgcccctgc
     7021 gtctgttgca gacaatacgc ccaggtttcg gctaagcact cacacgactt ctggctggta
     7081 atacccagac ttgcgatcca tttttccgga tcaaattcac cagccttatt gatatgtgca
     7141 cttcttaccg caaccatcgt cctctccttt agggaccaga cctgccgaaa tcggcaaatc
     7201 gtaactattt aacgcgcgag aaaagtacca tcgattccag atgtcccgtg tgtgggaaca
     7261 tatccagcat cgccagtcgc gcaatggtat atcctgcttt taataacgct tcgctatccc
     7321 gagccagcgt tgcaggatta caggatacat aaactatacg aataggttcc agttttataa
     7381 tttgctgcat aacacctgcg gcacctgctc gcgcggggtc cagcaacact ttatcgaagc
     7441 cgtttttcgc ccacggctgc tttgtgacat cttcttcaag attttcgtga taaaacgtca
     7501 cattctgtaa gccattaaga cgcgcattct gctggccttt ttccaccagc gccggaacac
     7561 cttctacacc caccacactg gcagcttgtg tcgccaatgg cagtgtaaag ttgcccatac
     7621 cgcagaacag atccagtacg cgatcttcag gttgcacatc cagccattcc aacgcacgcg
     7681 ctaccatttt ttggttcaca cccgcattga cctgaataaa atcgcgcggg ctaaaagtta
     7741 agcgcaaccc gtttgagtca taccagggca tctcaccaga gacggtttcg agtatctcac
     7801 tatcgggggc gagatacaga tccaggcctt cagaatgcga aaagcgttcc agtttttcgc
     7861 gatcagccga acttagcggt gcggtatggc gcaaaatcat cagcgtgccg ctggttgcct
     7921 gtaccagttc aacatgacca agatggcgta tagcttgtaa gctgcccagg catgccctga
     7981 ctttgggcag caatgcttca agttggggca ctaaaatggg gcattgtttg acgtcgacaa
     8041 tgtcactgga gcccgctttg cgaaagccca tctgaagttg ctgtgttttc ggtaagtagt
     8101 tcaaacttaa acgcgcgcga cggcgatagc cccagggaac atcggcgatc acttcagaga
     8161 cttcgtgttt cattaatcgg gcgagtgccg cacttttgct tcgctgctgt aaatccacgc
     8221 tggcgtgttg ttgctgacag ccaccgcata cgccaaaatg agggcagcgt ggcgtttcgc
     8281 gttccgggct atcgcttaac cggcgcacga ctttagcgcg ggcgtactgt tttttatctt
     8341 cagtaacagt aacttccgcg ttttcctgtg gcagtaatcc aggaataaat aacgctttgc
     8401 cgttatgtcg cgccacgccc tgaccaaaag agtcgaggtc gttgactgaa acggttatga
     8461 tctgacgcgt cgtcgtgcgt cgttttgcag agtagaattg cgccattggc gagactttct
     8521 caatttaaca gtgtgacctt aattgtccca taacggaact ccatgaccaa ctacagcctg
     8581 cgcgcacgca tgatgattct gatcctggca ccgaccgtcc ttattggttt attgctgagt
     8641 atctttttcg tcgtgcatcg ctataacgac ttgcagcgtc aactggaaga tgccggtgcc
     8701 agcattattg agccgcttgc agtttctact gaatatggca tgagcctgca aaatcgcgaa
     8761 tctatcggtc agttaataag cgtactgcat cgtcgccatt ccgatattgt tcgcgcgatt
     8821 tcggtttatg atgaaaataa ccgactcttt gtcacctcca attttcatct tgatccctca
     8881 tcaatgcagc tcggcagcaa cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc
     8941 gatattatga tcctccgcac gccgattatt tctgagagtt actcccccga cgaatcgccc
     9001 agtagcgatg ccaaaaatag tcaaaatatg ttgggatata ttgcgcttga gctggatctt
     9061 aaatcggttc gtttgcagca atataaagag atctttattt ccagcgtgat gatgctgttt
     9121 tgtatcggta ttgcgcttat ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt
     9181 cgcaacatgg tgaataccgt cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa
     9241 ggatttatgc tcggcgagct ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg
     9301 ctggctgctt atcacgaaga gatgcagcac aatatcgacc aggcgacgtc cgatctgcgt
     9361 gaaacgctgg agcagatgga aattcagaac gttgagttag atctggcgaa aaagcgcgcc
     9421 caggaagcgg cgcgtattaa atccgagttt ctggcaaata tgtcacacga gctgcgtaca
     9481 ccactgaatg gtgttattgg ctttacccgc ctgacgctga aaacagaatt aacaccaacg
     9541 cagcgcgatc acctgaatac gattgaacgt tcggcaaata atttgctggc aattattaat
     9601 gatgttctcg acttctcgaa actggaagca ggtaagctga ttctggaaag tattccattc
     9661 ccactacgca gcacgctgga tgaagtcgtt actctgctgg cacattcttc tcacgataaa
     9721 gggttggaac tgacgctcaa tattaaaagc gacgtgcctg ataacgtgat cggcgaccca
     9781 ctgcgattac agcaaatcat cactaacctg gtggggaatg caattaaatt caccgagaat
     9841 ggcaacattg atattctggt agaaaaacgt gcgctgagta ataccaaagt gcagattgaa
     9901 gtgcagattc gggataccgg cattggtatt cctgaacgcg atcaatcgcg cttattccag
     9961 gccttccgac aggctgatgc cagtatttcc cgccgtcatg gtggcaccgg tctggggctg
    10021 gtgattacac aaaaactggt taatgaaatg ggcggcgata tttcgttcca tagccagccg
    10081 aatcgcggtt caactttctg gttccacatt aatctcgatc tgaacccgaa cattattatc
    10141 gaagggccat ccacccagtg cctcgccggt aagcgcctgg cctatgtcga accaaactcc
    10201 gcagcagcgc aatgcacgct ggatatttta agtgaaacgc cgctggaagt ggtttatagc
    10261 ccaacgttct ccgcgctgcc tcccgcgcat tacgacatga tgttgttagg catcgcggtg
    10321 accttccgcg agccgctaac aatgcaacat gagcgattag cgaaagcggt atcgatgacc
    10381 gatttcctga tgctggcact tccttgccat gcacaagtca atgcagaaaa actcaagcaa
    10441 gatggtatcg gcgcgtgtct gctgaaacca ttaacaccta cgcgcctgtt gcctgccctg
    10501 acggaatttt gtcatcacaa acaaaacacg cttttgcctg taaccgatga aagcaagctg
    10561 gcaatgacag tcatggcagt tgatgacaac cccgctaacc tgaaacttat cggcgcattg
    10621 ctggaagata tggtgcaaca tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg
    10681 gcgaaacaga tgccgttcga tttgatctta atggatattc aaatgcctga catggatggc
    10741 attcgggcct gcgagctcat ccaccagctc ccccatcagc aacaaacgcc ggttatcgcg
    10801 gtaacggcgc atgcaatggc cgggcaaaaa gagaagctgc ttggcgcagg gatgagcgat
    10861 tatctggcga aaccgattga agaagagcga ttgcaaaatt tgttgttgcg ctacaagcct
    10921 ggcagcggta tttcctctcg cgtcgtgacg cccgaagtca acgaaattgt ggtgaacccg
    10981 aatgcgaccc tcgactggca actggcacta cgccaggcag caggaaaaac cgatttagcg
    11041 cgcgatatgc tgcaaatgtt actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa
    11101 cagctggttg gagaaaaccc ggaaggcctg gttgatttga ttcataaact gcatggcagt
    11161 tgcggctata gcggtgtgcc gcgtatgaag aatctctgcc aactgatcga acaacagcta
    11221 cgtagcggta ctaaagaaga agatttggag ccggagctgc tggaactgtt ggacgagatg
    11281 gataatgtag cgcgcgaagc cagcaaaatt ctcgggtaac ggacgtcttg tcgagtagca
    11341 atcggctttt tatgacgctg gtttcagatt ggtgacaaag tgcctgtgtt tatgccggat
    11401 gcggcgttaa cgccttatcc ggcctacaaa accaattaaa ttcaatgaat taaaaaatga
    11461 tgtagacctg atacatagca caacaggcta ttttgcgttt agcatcagtc tcaagccggc
    11521 tccagataga gccggttttg gttttctgtc ttaacgcacc atgcacgggc gcttgttatc
    11581 gaacgtccag ccaggaatca gatactgcat tcccatcgca tcgtcacgcg cgccaagccc
    11641 gtgtttctga tacagctcat gggctttcat cacttgatcc atatcgattt ctacacccag
    11701 ccccggtttt tccggcacct gaaccagacc gcctttgatc tcaaacggtt ctttggtcag
    11761 gcgctgatta ccttcctgcc agatccagtg cgtatcaata gcagtaattt tacccggtgc
    11821 agcggcggca acatgggtaa acatcgccag ggaaatatcg aagtggttgt ttgagtgtga
    11881 accccaggtc aggccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcatcgt
    11941 ccagaaatgc ggatccgcca gcgggatatc aacggattgc agggagagcg tatggcccat
    12001 ctgccgccag tcggtggcga tcatattggt tgcggtcggc aggccagttg cgcgacggaa
    12061 ctctgccatc acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata
    12121 agccagcgac cctttcaggt atttaccaat tttaatcgct tcgttcagcg accaggcacc
    12181 gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca caatagactc
    12241 ggcctcttct tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt
    12301 ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg
    12361 caggcgatac cagtcgcatg agtcatccgg ctggctttga tacggcagcg gtgtggcttt
    12421 gcgatcaccg acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc
    12481 gcccagcagc gatgccacgt ttacccccag atgctgcccc agcagatcca gcattgccgc
    12541 ttctatcccg gtaactacat gaatagtggt acgcaaatca aatgtctgca aaccgcgccc
    12601 gccggcatca cgatcggcaa atgtattacg caccagcgtc agaacgtttt tgtattcacc
    12661 cagcgttcta cccaccacca gcggaatcgc atcttccagc gttttacgga ttttctcgcc
    12721 gccgggaatt tcgcctacgc cggtgtgacc ggaattatct ttgataatca caatattacg
    12781 cgtgaagaac ggtgcgtgtg caccactcag attcatcagc atactgtcat gacccgccac
    12841 cgggatgacc tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc
    12901 ctttttcaga atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg
    12961 atcaggtact gcatcggacc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc
    13021 tcatgtgcct tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga
    13081 acggcaattt ttccgttttt aatctccagc ggatttttgg tcaggcgaca atcgccctcc
    13141 tgccaaatcc agtgggtatc gatagcggta ggattacccg gtgccgccgc gcccacatgg
    13201 gtaaacatcg ccagagaaat atcgaaatgg ttattagaat ggcagcccca ggttagcccc
    13261 cagtcgtcgc aaagctgtgc cacgcggact gcaccggaaa gcgtccagaa gtgcggatcg
    13321 gcaagtggaa tatctaccgc attgagcatc accgcatggc ccatttcgcg ccagttggtg
    13381 acgatcatgt tagtcgcgac gggcaagccg gtcgcccgtc gaaactccgc catcacttca
    13441 cgaccggaga aaccctgctc agcgccgcac ggatcttcgg cataggtaag aacatcattc
    13501 agccctttgc acaaagaaat ggcttcatca agcagccatg caccgttggg atcaacggta
    13561 atccgcgcat ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca
    13621 ggtaacactc cgcccttaag tttgaaatct ttaaagccat agcgatcctg tgaggcttcc
    13681 gccagacgca caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca
    13741 tggttgcccg gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa
    13801 aacagataac cgaggacggt aatagcatcg cgttgcttgc ctggccctaa cagttcgcag
    13861 accggaacat tcaacgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg
    13921 gcattaacgc gcaattcaaa agtccaggca cctttgccga aggtatcaaa atcggccgcc
    13981 tgattaccta tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctggcccagc
    14041 accatcggaa tagcatcgac aagcgtctga taaatcacct ctccgcccgg cgcttcacca
    14101 ataccggtat gcccggcatt atcggtgagt accacaatat tgcgggtgaa atatgcgcta
    14161 tgtgcaccac caatattaag caacatgcta tcgtgcccgg cgaccggaat gactttcata
    14221 tcagtaataa caggactgga ttgtgtcgtc atcttattgc cccgcgacag gtttcagctc
    14281 gatacgcttg atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac
    14341 accaacataa atcagcgccc cattaaagga gccagtcgtg ccaacgatat aaccaattgc
    14401 gattggcgtg acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc
    14461 gctgatctct tttggcgcgg tatctgccat tactgcccaa cccagcgccc cgatgccctt
    14521 accgaagaag gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca
    14581 gaacaccatc accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt
    14641 cagcgatccc gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccgccgat
    14701 aaaaccgcaa accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccacg
    14761 cgcctgcacc agataaaccg ggaaccaggt aataaagaag taagtcaggg cgttgataca
    14821 gtactgaccg atataaacgc cgatcatcat ccgtgacccg agcagctgtt tgatctgccc
    14881 ccacttcacg ctgaacggca ctttaacttt ggtgttttgc tgatccatat tgatcagcgc
    14941 gccacccgcg gcgatgtact ccagctcttt ctgatttacc cccggatgtt gatttggctc
    15001 gtggatgact ttcaaccaga taaagctgat gacaatcccc agaccgccca taaagaagaa
    15061 gacgtgtgac cagcccactt catgcgtcag ccagcccata atcggcgcga agatcaccgt
    15121 tgcgaagtat tgagcggagt taaaaatcga caccgccgtt cccctttcct gcgccggaaa
    15181 ccaggccgca acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag
    15241 gaagcgcaga gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc
    15301 ttgcagcaag gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc
    15361 aaaacgatcc agcaaccaac caccagggat ctgcccgata acataagccc atgagaaggc
    15421 agagaacaca tagcccattc ccacgggatc aaggccgata tctttggcca tttccgaacc
    15481 ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac
    15541 cactatccag taacgagcat tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga
    15601 actcattgtt gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatag
    15661 ccaggaaatc agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat
    15721 acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt
    15781 gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt
    15841 ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat
    15901 aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta
    15961 tgaagcagtt tttactggca tttgcctgaa aatattcgat tcagcaccgc taaaacgaca
    16021 tttaccgctc gctgaacata tcaggacaac agcgtgcccc actgttcgac ccacggattt
    16081 gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc
    16141 tgagcgctct gttcctgcaa caaggcatcg aactgtttgc cgccattgca gaaatttaca
    16201 taactgctgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta
    16261 tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg
    16321 gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa ttcaggatct
    16381 tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact
    16441 aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct
    16501 ccctggatta ggaacttatc tctccatcct gacccgatgg cgcactgaac tcaacccttt
    16561 cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt
    16621 ccaggcccgc gtgaatagtc agcggctcgc cagtaaaagg atgcgtcaat gacaactgac
    16681 tggcatgtag cattaaccgc tggaggccaa aatgctcagc accgctgcga ttctggcgta
    16741 aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa
    16801 gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt
    16861 aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg
    16921 gctgcgagcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca
    16981 gtggataatc cagaactgct tcttccatca accagccgcg cacaatcgca tggtaacgtt
    17041 tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc
    17101 ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcggtaaaa acgtgctggc
    17161 caatctgatc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctgc
    17221 ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta
    17281 tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca gctcagccag
    17341 aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacggc
    17401 aaaagcgccc ggtaacggct gtttgttatc taacaaatcg tgcattcgcg ggatcagcac
    17461 ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa
    17521 ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag
    17581 cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa
    17641 actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag cgctcccggt
    17701 tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct
    17761 ctggccttgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg
    17821 tgatacttca tcctgaagtg tccctggcct tcctgaccca ccgaatcatc ctgaccggtt
    17881 ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc
    17941 cagaagcctt tccctgaaac accatcctgg tgtgtcctgc agaagtgtca tcatcctgat
    18001 gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct
    18061 tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta agaattaaca
    18121 atcaacattt tgattttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt
    18181 tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga
    18241 ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg
    18301 tttacgtgtt cccagcgtct ctttgcacac ttcaccggag agattacaca ccgagatgac
    18361 ctctaactct tcttccagcg tcgcgataaa tagcgtgggc ggcagtttta gccttttctg
    18421 agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt
    18481 ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt
    18541 cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt
    18601 tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt cggtagttga
    18661 aataatgcac ggtgaaggaa caccgtacag ttcctcactt aacggccagc ttttatgctc
    18721 ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc
    18781 gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt
    18841 atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg
    18901 aaaatcaacc gattaccggg atacctatga cgccaaccta ctgcaaggcg ttccacgcag
    18961 cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat
    19021 ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg
    19081 tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta
    19141 tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga
    19201 gcgcgactta agcacttgcg cccagggtaa ggttagcgtg gcgttatatc gtcttgatga
    19261 actggaaggc cagccgatag gtcatttcaa tggcacctgc attgatgacc aggatatcac
    19321 tatcgataac tatgaattca ctactgacta tctggagaat gccaccagcg gtgaaaaagt
    19381 agtggaagag acgcttgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc
    19441 agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct
    19501 gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat
    19561 ctttaatgac ctgttacgct tctgccagcc agagaaatta agcgtttacg cacgttatac
    19621 ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac
    19681 cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag
    19741 gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat attcgtccca
    19801 taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca
    19861 gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa
    19921 ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc
    19981 tcgttttgaa aatttcgata ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact
    20041 gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa
    20101 cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata ataccgttcg
    20161 ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt
    20221 gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg
    20281 tcactcaatt aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc agctgggcct
    20341 gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg
    20401 tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga
    20461 gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc
    20521 ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca ttatcttccc
    20581 tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc
    20641 caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt
    20701 ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc gccattaccg
    20761 catcatcatt gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt
    20821 gaaagaaaat cgccgcgaca ctggtgacgc ttacagcttt aactggtcaa tgcgcattgc
    20881 accagatttg caaatgccgt ttgagccgtc tcacgagaat atggctaatc tgaagcttta
    20941 cccggatcaa cctgttgaag tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt
    21001 ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat
    21061 caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca
    21121 gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc
    21181 tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa
    21241 ctcatttcat ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag
    21301 ttaatatgtc atacaattta tattgcaacg caaacgtttc cctattttca taaaccgtat
    21361 tttatcgcta tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt
    21421 tcaacctaca tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt
    21481 gcccttaaat tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc
    21541 caagtagatt attgtatttg agatcaagat cactgataga tacataactt gtgtgtatct
    21601 ttccgccctc aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca
    21661 tttttacggt caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc
    21721 tccaggagaa atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta
    21781 gtgcctggcg caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg
    21841 cgggcgtgct gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca
    21901 tggctatcct tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac
    21961 tgtctggtaa aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg
    22021 gcgcaggtaa actgattacc ctgctctatt tcttcgctat ctacccgatc ctgctggttt
    22081 atagcgtagc aatcaccaat acagttgaaa gcttcatgtc tcaccagctg ggtatgacac
    22141 caccgccgcg tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct
    22201 tcggtgagca gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac
    22261 tgatgctgct ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt
    22321 ctctggacac tgcatctgca accgggaacg gtctgtggat gaccctgtgg ctggcaattc
    22381 cggtaatggt gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc
    22441 gtgaagagta cggcgatatg gcagaacaga aatgctcgaa gatccttgca ttcgcacaca
    22501 tcatgatggt gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg
    22561 cagacctggc tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact
    22621 ttaacgcacc ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat
    22681 ccttcctcgg ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc
    22741 tgcgtggtaa aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca
    22801 tgctggtaac gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa
    22861 ccctgggcgg cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga
    22921 aagtaccggc aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg
    22981 gtctgattgc aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct
    23041 ttcgggcggc gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata
    23101 ttttcaaaat cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta
    23161 aacaatttac cgacgatctg attgcccgta atctgcttaa agacgtgacc cgcgtggtgg
    23221 ttgacgtgta cggctcgctc tctctgaccg gtaaaggcca ccacactgat atcgccatta
    23281 ttatgggcct ggcgggtaac ctgccggata ccgtggatat cgattccatc cctggtttta
    23341 ttcaggatgt gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt
    23401 tcccggttga tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta
    23461 tgcgcattac cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg
    23521 gcggtggctt tatcgttgat gaagagcatt ttggccagca gaatagcgca ccggttgaag
    23581 ttccttatcc gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt
    23641 cactctctgg cctgatgatg aaaaatgagc tggcgctgca cagcaaagaa gagctggaac
    23701 agcacctggc gaacgtatgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg
    23761 aaggcgtgtt gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc
    23821 tggtcagcca ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt
    23881 ttgcactggc agtgaacgaa gagaacgctg ctggcggacg cgtggtgact gcgccgacta
    23941 acggtgcgtg cgggattatc ccggcagtgc tggcgtacta cgacaagttt atccgcgaag
    24001 tgaacgctaa ctcactggct cgttacctgc tggtagccag tgccattggt tctctttata
    24061 agatgaacgc gtcaatttct ggtgctgaag tcggctgcca gggtgaagtt ggtgtggcgt
    24121 gctcaatggc ggcggctggt ctggcagaac tgttaggtgc aagcccggcg caggtgtgca
    24181 tcgcggcgga aatcgccatg gagcacaacc tcggtctgac gtgtgacccg gtcgccggac
    24241 aggtccaggt gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg gtgaacgccg
    24301 cacgtatggc gctacgccgt accagcgagc cgcgcgtctg cctcgataaa gttatcgaaa
    24361 ccatgtacga aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct cgcggcggcc
    24421 tggcaatgaa gatcgttgcc tgcgattaat cgctctccag aggcctcgtt ttgcgaggcc
    24481 tcttcccgat ttctcatcca gccgtagcct gttccggcat cgaatgttac ccttatcgcc
    24541 tgatctttaa gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta
    24601 ttcgtcgcat tcatgccgtt cagggatcgc cctgtgtcga aacctgccag catgcgctcg
    24661 atcagctcat tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc
    24721 gcagtagcgg ctggcgtcat cagcgtttac cagattacaa agcgggtcga ccgccaatgc
    24781 cggaagagtt gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc
    24841 cgtgctggtc aaccagcggc aacgaagccg atgacttagc cgccacgctg gcggtcaaag
    24901 tgacacaggc cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac
    24961 tttcaccgac attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta
    25021 tcgataaaga atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga
    25081 tcagcagttc aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc
    25141 tggtcgagtt tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt
    25201 ggcgcaaaaa attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct
    25261 tacaaaccga tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc
    25321 gagccggata cgccgcaaac gtcgtatccg gcattatcac atcagcgcat ttaccaggcg
    25381 gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc accggtacaa
    25441 acatcatcca gtgccgcctg cgccagtgcc ggaatgtctt ccttgcgtac cccaacatca
    25501 cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc aacagcggca
    25561 ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg cgcgatatcg
    25621 cggtacttct caccggtaaa gtcagcgtta tagcgcatga catgcggtag caggatggcg
    25681 tttgcaacac cgtgtggagt gttataaaac gcgcccagtg gatgcgccat accatgcacc
    25741 aaccctaacc caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct
    25801 tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc
    25861 gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata
    25921 gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc
    25981 atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg
    26041 cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc agtgcctgct
    26101 gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag tccttccagg
    26161 ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc
    26221 tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg
    26281 agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat
    26341 gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc
    26401 gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa
    26461 gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc
    26521 atccttctcc ttgttgcttt acgaaattac tcttcaattc gtaacccata ggttttgaat
    26581 ttctccagca ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc
    26641 agggtcgtca ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt
    26701 tccagattca cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt
    26761 ttgagagcct gcgcaacatg ttcagaaagt tcacgtgttc caaaggtcgc ataaggcgcg
    26821 caaggaatag aattaccgcc agccgccgca atcatgtagt gaatagcggg gatcgatcgg
    26881 ttaagaatgg aaactgccgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc
    26941 ggtctgcttt gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc
    27001 tcatgtttac cgttgccatc aataaagaca atatgcgact ccgtcagttt ttcatatgga
    27061 atgcctgtag gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc
    27121 ccctggttca gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt
    27181 ttatttcgtt ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt
    27241 tcacactatt gaattagccg tttaattacc caccatcttc ttcctgatta accagaaaga
    27301 aattcacaag cttatatttt gtgacctggt tcaactaatc acagtaaata actgcaagtt
    27361 ctctttttat aaccccatta aaaatgaccg ctcttaaaaa tatttatcaa aacggtcatt
    27421 tttctattca ctcaagccag gaatgcccgt tttcggcata aacaattaat acggtcatct
    27481 ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat tattaaagtg
    27541 atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca tattacagag
    27601 cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata ttcactgata
    27661 tgaagcccga actcgctggt tttgcacttt tgaaaacata accgattacg tgcttaagct
    27721 tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt taccgtgcgg
    27781 tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg ctgtgctcac
    27841 tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct caattccagc
    27901 aggcttttac gctgacaaat ttccaggctg gcctgatcca atcggccttt tactttggtt
    27961 atttcattat cccaatccct gctgggatat tgatgaaaaa actcagttat aaagcaggga
    28021 ttattaccgg attattttta tatgccttgg gcgctgcatt attctggccc gctgcagaaa
    28081 taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga ttaggttgtc
    28141 tggaaactgc cgcaaaccct tttgttacgg tattagggcc ggaaagcagt ggtcacttcc
    28201 gcttaaatct tgcgcaaaca tttaactcgt ttggcgcaat tatcgcggtt gtctttgggc
    28261 aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat aaaatgtctc
    28321 cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca ccttatatga
    28381 tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa ttcccggcat
    28441 tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg ctttctcgtc
    28501 tggcgcgtat tcgtcactgg cgctgggcgg tattagcgca attctgctac gttggcgcac
    28561 aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcca ggtatgactg
    28621 caggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt ggtcgtttca
    28681 ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc tacgcattaa
    28741 tcgctatggc actgtgcctg atctcagcct tcgctggcgg tcatgtgggc ttaatagccc
    28801 tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg ctgggcatta
    28861 agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc atcattggcg
    28921 gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac atccccactg
    28981 ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt ttccgctctc
    29041 aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa tgaaaaaaat
    29101 cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg gtgttcgtga
    29161 gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc tgaccgagaa
    29221 actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct gtatcgcggg
    29281 tatggctgaa gccgctgctt gcgaagaaaa attcagcagt cagaatgtag gcctcaccat
    29341 tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc caacccgccc
    29401 gaaagccatt tggggcttta acggcactga acgtcccggc gctgtttacc tggcagcggc
    29461 tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc atgacgttca
    29521 ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc gctttgcccg
    29581 cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg gcggcgtttc
    29641 gatgggtatc gccggttcta ttgttgatca caacttcttt gaatcctggc tgggaatgaa
    29701 agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga tttacgacga
    29761 agccgaactg gaaatggcac tgacctgggc tgataaaaac ttccgctatg gcgaagatga
    29821 aaataacaaa cagtatcaac gcaatgccga gcaaagccgc gcagttctgc gcgaaagttt
    29881 actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg ccgatattgg
    29941 tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg ggcaacgtca
    30001 ctggaccgat caatatccca atggtgacac cgccgaagcg atccttaaca gttcatttga
    30061 ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc ttaacggcgt
    30121 ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg atgtgcgtac
    30181 ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactcgatg gactggcaga
    30241 acacggcatc atccatttga tcaactccgg ttctgctgcg ctggacggtt cctgtaaaca
    30301 acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc agcaagaggc
    30361 tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact tccgtggcgg
    30421 cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga cccgtgtcaa
    30481 catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg tggaattgcc
    30541 gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa ccacctggtt
    30601 tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg tactcggtaa tggcgaactg
    30661 gggcgctaac catggggttc tgaccatcgg ccacgttggc gcagacttta tcactctcgc
    30721 ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag tgtatcgtcc
    30781 ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg cttgccagaa
    30841 ctacggcccg ttgtacaagc gttaatacat tactccctga tgtgatgccc ggtcgctccg
    30901 gctaccgggc ctgaacaagc aagagtggtt agccggataa gcaatgttat ccggctatat
    30961 tgcaggagcg attatgaaac aagaagttat cctggtactc gactgtggcg cgaccaatgt
    31021 cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa cgcctaatgc
    31081 cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagacg ccattttgca
    31141 acgctttgct gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc acatccgcgg
    31201 tatcgccgtc accacctttg gtgtggatgg cgctctggta gataagcaag gcaatctgct
    31261 ctatccgatc attagctgga aatgtccgcg aacagcagcg gttatggaca atattgaaca
    31321 gttgatctcc gcacagcggt tgcaggctat ttctggcgtc ggagccttta gtttcaatac
    31381 gttatataag ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac gcgcgcacgc
    31441 ctggctcttt atttcgtcgc tgattaacca ccgtttaacc ggcgaattca ctactgatat
    31501 cacgatggcc ggaaccagcc agatgctgga tatccagcaa cgcgatttca gtccgcaaat
    31561 tttacaagcc accggtattc cacgccgact cttccctcgt ctggtggaag cgggtgaaca
    31621 gattggtacg ctacagaaca gcgccgcagc aatgctcggc ttacccgttg gcataccggt
    31681 gatttccgca ggtcacgata cccagttcgc cctttttggc gctggtgccg accaaaatga
    31741 acccgtgctc tcttccggta catgggaaat tttaatggtc cgtagcgccc aggttgatac
    31801 ttcgctgtta agtcagtacg ccggttccac ctgcgaactg gatagccagg cagggttgta
    31861 taacccaggt atgcaatggc tggcatccgg cgtgctggaa tgggtgagaa aactgttctg
    31921 gacggctgaa acaccctggc aaatgttgat tgaagaagct cgtctgatcg cgcctggcgc
    31981 ggatggcgta aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct ggcaaggagt
    32041 gacgcttaat accacgcggg ggcatttcta tcgcgcggcg ctggaagggt taactgcgca
    32101 attacagcgc aatctacaga tgctggaaaa aatcgggcac tttaaggcct ctgaattatt
    32161 gttagtcggt ggaggaagtc gcaacacatt gtggaatcag attaaagcca atatgcttga
    32221 tattccggta aaagttctcg acgatgccga aacgaccgtc gcaggagctg cgctgttcgg
    32281 ttggtatggc gtaggggaat tcaacagccc ggaagaagcc cgtgcacaga ttcattatca
    32341 gtaccgttat ttctacccgc aaactgaacc tgaatttata gaggaagtgt gaaatgctga
    32401 aaacaatttc gccgttaatt tctcccgaac tattgaaagt gctggcagag atgggacatg
    32461 gagatgaaat tattttttcc gatgctcact ttcccgccca ttcgatggga ccgcaggtga
    32521 tccgcgctga tggcctgttg gtgagcgact tgctccaggc gattatcccg ttatttgagc
    32581 tggacagtta tgcaccgccg ctggtgatga tggcagcggt agaaggtgac actctcgatc
    32641 ctgaagtaga acgacgttac cgtaatgcgc tttcactaca agccccgtgt cctgacatca
    32701 tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg atcgttatca
    32761 caggcgaacg agcgaagtac gggaatattc ttttaaaaaa aggggtaaca ccgtaatctc
    32821 ataccggtac gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa aatatgaaag
    32881 cggcacgcca gcaagcgata gtcgacctgc tgctgaacca taccagcctg accacggaag
    32941 ctctctctga acagctaaag gtcagtaaag aaaccattcg tcgcgatctc aatgaattac
    33001 agacgcaggg taaaattctg cgcaatcatg gacgcgctaa atatatccac cgtcaaaatc
    33061 aagacagtgg cgatcccttt cacatccggc tgaaaagcca ttatgcgcat aaagcagata
    33121 tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt gatagcctta gacgccagtt
    33181 caacttgctg gtatctggca cgccagttgc ctgacatcaa cattcaggtc ttcaccaata
    33241 gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc agttccggcg
    33301 gcacacttga gcgcaaatat ggctgttacg tcaatccctc gctgatttcc caacttaaat
    33361 cgctggaaat cgatctgttt attttttctt gtgaagggat cgatagcagc ggcgcattgt
    33421 gggactccaa tgccatcaac gctgattaca aatcgatgct attaaaacgt gccgcgcaat
    33481 cgttgttatt gattgataaa agtaaattta atcgttcagg ggaagcccgc atcgggcatc
    33541 tggatgaggt aacgcacatt atttctgatg agcgccaggt tgcaacttct ttggtaacag
    33601 cctgacggaa agggtagcag gccggagacg acgccccggc cttgcctgtt atcgctcgtc
    33661 gcgacgacca cccaccgcag cccagatgcg gcggacgtgc accgtcactt cttcgcgatc
    33721 gtgatacaac tgccgcgcct gaatctgagc atttatgcca tgttcatcaa gctgtgcctg
    33781 aatatacgcc agattgtgtg acacttcttc gtagcgtttt ttcatcggca gtttgaggtt
    33841 gaaaatggtt tcacggcacc agccattaac cagccactgc gccatcaatg ccgcaacttt
    33901 cgccggtttt tcaaccatat cgcataccat ccaggagata ttgctgcgcg tcggacggaa
    33961 tttgaaaccg tcttcccgca gccacgtcac ctgtccggta tccatcagac tttgcgccat
    34021 cgggccgttg tcgacggaat aaacccacat gttgcgcttc accagttggt aggtccagcc
    34081 gccagggcaa gcgcctaaat ccaccgccca catcccgttc gccaggcgtt catcccactc
    34141 atccgcagga ataaacacat gaaatgcctc ttccagtttg agcgtggaac gactcggcgc
    34201 atctgccgga aatttcaggc gcggaatgcc catatagaac ggcgaattat tgttgctgta
    34261 tgagtaaccg gtatagcagc agcctggtgc aataaagaat acatgcacaa ccggacgctt
    34321 cggcgtttca tagttcgcca gcaccccggc atcgcgcaag gcagcgcgta gcggaacagt
    34381 aaatttacgg cagaatttca gtaactcttt gctttcgttg gtatcggcaa cttcaacacg
    34441 cagttcaccg cccttctcta ctacgccctg taacatgccg acaatggggg taatacgatc
    34501 ttctggcggc aaatgctgca ggagttcccc caccacaaac cactggcggg caaaaattaa
    34561 tgaactgaac ggcagctcac ggattaactt atcgccatca tcaggttgat aacattcata
    34621 aatgacataa cccgcattct ctttcacgcg ggcaaaaccg aaaatttccc gctggccggc
    34681 tttatcggta atttctgcgg cgcactcttt ttcaaagccc ggacggcaca gcaatacaac
    34741 cttattcatg acttacgccc ttgcgcttta aacggatagc accaactaac attaacgccc
    34801 agcccgcgag gaagctcacg ccgccaaccg gagtgacaaa cgcccacaaa cgcagatggg
    34861 acagcgccag gcaataaagg ctgccgctga acaacaccgt gcctaacgcg aggaaaacgc
    34921 tactccagta aaaccagata ctgatgcgac gctgcattgc caccgccaga cctaagatcg
    34981 ccagcgtatg aaacgcctgg tattcgaggc cggtctggat ccagcccatc tcaacggccc
    35041 ccatggtttt acttaacaca tgcgcgccaa aagcgcccag agccacaaaa atgaagccgc
    35101 taatggcggc gaaaatcagc atgaaacggc tggtcatggt cgtaccctac gtaaattatt
    35161 gttcataacg aaagcggaat ttttcttgtt cagcagcggc tttcgccagg atccattggc
    35221 gaaaggcggc tattttaccc agttctgcct ggctgtcatg acaaaccaga taaaaagcgt
    35281 ttttactgac cagaacatca ttaaacgggc aaacaagacg tccggcctcg atttcagatt
    35341 gcgccatcac gttatttgcc aacgccactc cctgcccgtg aatagccgct tgcagcacca
    35401 tggcgctatg actaaaaatt ggcccttgct gaacgttgat atgatttaac cccaactgtc
    35461 gggtatatgt ctgccagtca cggcgtgacg catcatgtaa taacgtatgt ttagccagat
    35521 cttccggggt cttcaagggt ttttcgcctg tcagcagtag cggcgaacac accggcaata
    35581 aatattcggc gtacagtttt tccacccgta gccccggcca gttgccccga ccataaaata
    35641 tcgccacatc aacatcatcc gccagcttat cttcctgacg atcaaccgcc tggattcgaa
    35701 cgtcaattcc cggataagct gaattaaagc tggaaagtcg cggaaccaac caatgaatgg
    35761 cgaaactggg gagtaaactg accgtcaacg cccccttggc gctacgggcc tggagtttac
    35821 gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc gaggaaatag ctttgccctt
    35881 cctcggtcag caggagtgaa cgattacggc ggcggaacag ttttagcccc aaaaaatcct
    35941 caagagactt gatttgatga cttactgcgg cctgggtcac aaaaagctct tctgctgcgc
    36001 gagtgaaact taaatggcgt gctgcggcat caaaaactcg taaggcattt agcggtggta
    36061 atcgtttaga catggctttt aaactttgat gttaaatgaa tttaacaatt agatcacact
    36121 gtgtaaccta ttagtttttt taatctgagc cattataaat tgtccgttga gcttctacca
    36181 gcaaatacct atagtggcgg cacttcctga gccggaacga aaagttttat cggaatgcgt
    36241 gttctggtga acttttggct tacggttgtg atgttgtgtt gttgtgtttg caattggtct
    36301 gcgattcaga ccatggtagc aaagctacct tttttcactt cctgtacatt taccctgtct
    36361 gtccatagtg attaatgtag caccgcctaa ttgcggtgct tttttttacc ttgcgatcgc
    36421 gaattactga tccagttcga ccatctcttt cacgtcagta cggttgatct gctgtttgtt
    36481 gccattagcg tctttatacg aaatcatacc ggtatcgtta tcagtctgtg gtttgccgtc
    36541 agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg aacaggcgct
    36601 cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca tcatcttctc
    36661 cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta acgcctaaca
    36721 ctattcagca tatgttactt gcgcggcttc gccaggatat ccagataatt ctgatggtta
    36781 gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag ccgaggagta
    36841 ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgcacta caggatgcgg
    36901 gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt gaagccaccc
    36961 aacagtttta cagtctgagc gccggaaacg tccatcgcag ccagtttgcc gaagcccaac
    37021 gcctgaccgc gcgttatgaa gctgcacgag agaaagtggc gcaattactg aatgcaccgg
    37081 atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg gtggcacaat
    37141 gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg gcagaacacc
    37201 acgccaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa gtggtgaaat
    37261 tgccgcttaa tgcgcagcga ctgccggatg tcgatttgtt gccagaactg attactcccc
    37321 gtagtcggat tctggcgttg ggtcagatgt cgaacgttac tggcggttgc ccggatctgg
    37381 cgcgagcgat tacctttgct cattcagccg ggatggtggt gatggttgat ggtgctcagg
    37441 gggcagtgca tttccccgcg gatgttcagc aactggatat tgatttctat gctttttcag
    37501 gtcacaaact gtatggcccg acaggtatcg gcgtgctgta tggtaaatca gaactgctgg
    37561 aggcgatgtc gccctggctg ggcggcggca aaatggttca cgaagtgagt tttgacggct
    37621 tcacgactca atctgcgccg tggaaactgg aagctggaac gccaaatgtc gctggtgtca
    37681 taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag gccgaaagct
    37741 ggagccgtag cttagcaacg ctggcggaag atgcgctggc gaaacgtccc ggctttcgtt
    37801 cattccgctg ccaggattcc agcctgctgg cctttgattt tgctggcgtt catcatagcg
    37861 atatggtgac gctgctggcg gagtacggta ttgccctgcg ggccgggcag cattgcgctc
    37921 agccgctact ggcagaatta ggcgtaaccg gcacactgcg cgcctctttt gcgccatata
    37981 atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg gaattattgg
    38041 tggattaatg acaaacccgc aattcgccgg acatccgttc ggcacaaccg taaccgcaga
    38101 aacgttacgc aataccttcg caccgttgtc gcaatgggaa gataaatatc gccagttgat
    38161 catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg ctaaagagat
    38221 tgccggatgc gaaaaccgcg tctggctggg atataccgtg actgaaaacg gtaaaatgca
    38281 cttctttggc gacagcgaag ggcgcattgt gcgcggcctg ttggcggtgt tgttgactgc
    38341 cgttgagggg aaaaccgtcg ccgaattgca ggcacagtcg ccgctggcat tgtttgatga
    38401 gctgggattg cgtgcacagc ttagcgcctc acgcagccag gggttgaatg cgttaagcga
    38461 ggcgattatc gctgcggcga agcaggttta agcacagaac caggtcggat aagacgttcg
    38521 cgtcgcatcc gacctgattg tttaaccctg acgcgccgct ttcgccatca tcttcttcag
    38581 cgcatgagaa accgcaacaa acccaaaggt ggcggtcacc atcgttgccg cgccaaatcc
    38641 agatgcacaa tccatccgct tcggcccttc tgccgtggct ttcatcgcac acacagtacc
    38701 gtctgactgc gggtacacca gcgcttcagt agaaaacacg caatcgacgc cgagcttacc
    38761 tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcgcgcaact tcgccgccag
    38821 cggatcctga atcgttttcg ccagatcggt aacctgaatc tgcgtcgggt caatctgccc
    38881 acccgcgcca ccggtcgtga ccagcgggat tttattgcgc cgacaataag caatcagcgc
    38941 cgctttgggc cgtacactat caatggcatc aatcacgtac gaataaccca cgctcatata
    39001 ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacggc actccgggtt
    39061 aatctggcga atacgctccg ccatgacttc cgcttttgcc agcccgacgt tatcgcgcag
    39121 agcgtgaatt tgccgattgg tattggtgac gcacacatca tccatatcga taagcgtgat
    39181 tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac caccgatacc
    39241 aaccacacaa atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat acagacgcgc
    39301 tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag cacctcagaa
    39361 ttaaaaaggg tgaggttatc cctcaccctg gcgtatccgt aaaaacggta tacagaataa
    39421 cacatcagcc gctaaagacg ttacctgcgc ccggggcggt tttcagcacc cagacacgtc
    39481 catagtggtt gtaccaacct gcgcgatgtc cggcttccgg cccgatccct tgatagatat
    39541 cgaagtgttg gcctttgatt gcgccaccga catccagcgc caccatcaga cgcaattcgt
    39601 actgaccgtt aaatttgccg ttattatcca gcagcggcac ttctgccagc aaggtagtac
    39661 ctggcggaat aattgaacga tcggaggcaa cagaagcacg gccaaccagc ggcaccgcac
    39721 ttgccccttt caccggtgca aaagattgcg gtttaaagaa aacgaaagac gggttctgtt
    39781 ccagcagctc gcgaacctcg gcttcactgt gtgtttcgcc ccaatgacga atcgcctgca
    39841 tcgacatatc ttcttttttc acttcgccac ggtcgatcag caccttacca atgctgcgat
    39901 aggcatgacc gtttttccct gcatagctga aaaagttaag cggactgcca tcaccaaagt
    39961 cgatataccc gctaccctgc acatccataa tgaagttatc catcagggag ttgctgtaag
    40021 cgagaatata tttatcactc agcgcccctg cgtagatctc cgcacgagac ggcagacgac
    40081 cacgttttgg cggcatacgg taaatcggat actggaactc gccctggcgg gtatggcgcg
    40141 cctgaattac cggcgtgtaa tagcctgtaa actgcacgtt accatagttg tcggcacctt
    40201 ccatctgcca ggcatcaatg ccgaactggc gcatattgcg ggtatcaccg cctgcgcgca
    40261 gccactcttg caccgcgtta taaacattac tctggttgcc atacagacgc ggtgacgaat
    40321 tacggatatg gttaatttgc tcggcaaaat caccggcgtt aatcggcgcg ccaacggcat
    40381 ctggctggtt caccagagag aaaggctggg taaatttccc gtctttatat tgctgtccgc
    40441 gatcggttgg tttggaagag caggcggcaa gcattgccac aaccgtgccc ataagaaggt
    40501 actttaccca acgtcctttc attgttctct tcttcaggtt aaaaaataag gcgcaacgaa
    40561 gataacaaac cgccgcgttt aatgaaatga gcagcctctc cctgacgcaa attttgcaca
    40621 aaaaataggc tttagtgatt tgtttttgtt caaaatcatg ccaaatccgt gatcggggta
    40681 aaaaaaaggt tgcatgaaaa cgcgagcgga gtatagtgcg catccacgga cgcggggtgg
    40741 agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg tcggttcaaa tccggccccc
    40801 gcaaccaatt aaaatttgat gaagtaaagc agtacggtga cgcggggtgg agcagcctgg
    40861 tagctcgtcg ggctcataac ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatc
    40921 aaatttgatg aagtaaaagc aatacggtga cgcggggtgg agcagcctgg tagctcgtcg
    40981 ggctcataac ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatt attgaacacc
    41041 ctaacgggtg tttttttgtt tctgctctcc cataaaaaaa gcgccattca gcgccttttt
    41101 atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatt cccgcaagaa
    41161 tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt
    41221 tactgataaa cgccgtttcg accagaatgg agggaatatc aggcgctttc agcacggcaa
    41281 acccggcctg ttcaacctga tttttatgca gcttgttgat tttaccgagc ttattcagca
    41341 ccgctttacc aaacttaagg ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg
    41401 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct
    41461 gggtctgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag agcgcaaaca
    41521 ctgaggaacc gctcggctgg cgactggtaa aggcgtcggc atggatagag acaaacaggt
    41581 cggcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
    41641 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc agacggcgag
    41701 ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt
    41761 cgccgccgtg gccagggtca agcataataa caataggacg atcgcgccct gctttacccg
    41821 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
    41881 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
    41941 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
    42001 gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact
    42061 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
    42121 ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac tgcttatatt
    42181 tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc cagacgcgca
    42241 ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat agccacatag
    42301 cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
    42361 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
    42421 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
    42481 cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca
    42541 gaataaaaat acaataattt cgaataataa tgcaaagggg tgtgccgtgg taaaggaacg
    42601 taaaaccgag ttggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
    42661 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
    42721 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
    42781 acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt atcacaagaa
    42841 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
    42901 actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca
    42961 tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
    43021 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
    43081 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
    43141 taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag ctgaaaagat
    43201 gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg tctcagaact
    43261 tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg attacaactc
    43321 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
    43381 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
    43441 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
    43501 tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
    43561 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac
    43621 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
    43681 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
    43741 taccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
    43801 tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc ccgagagcaa
    43861 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc
    43921 gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg caattacgtt
    43981 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt
    44041 gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
    44101 gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
    44161 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc
    44221 tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
    44281 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
    44341 ctgtcattac gggcaatcat taccggtcga ccttcgtacc aacgagagtg cggatgacga
    44401 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
    44461 aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg aatgattaaa
    44521 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
    44581 tcaagcatcg caatataatc ttctccgctc tgtaaaagcc gtttttcgat atcagtaaaa
    44641 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
    44701 tgaccaatgc cagaatcact gccgaaacga tagctttttt gcagcaggca gagactgtcg
    44761 cgcaaagatg ccgcttctgt gccagttcct gccggaacgt gagttcccgt caggcggctt
    44821 agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat atcgcccagc
    44881 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
    44941 tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
    45001 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
    45061 cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat gcgtttcttt
    45121 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
    45181 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
    45241 attaacgctg ccagtaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
    45301 atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
    45361 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
    45421 gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc
    45481 aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct gctgaccgct
    45541 tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
    45601 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt
    45661 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
    45721 cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct
    45781 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
    45841 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat
    45901 gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga cgatcatagt
    45961 cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt
    46021 gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac
    46081 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
    46141 ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc
    46201 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
    46261 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
    46321 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
    46381 acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
    46441 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
    46501 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
    46561 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
    46621 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agtgtcctcg
    46681 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
    46741 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
    46801 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
    46861 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
    46921 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
    46981 cgaggtcgac gctttctggc gcagcgttaa gatccaaaac tgcctcaaac gagtggcgat
    47041 cgtgataaaa cgcctgatcc tggacgcgga aattggtgat aaacggcagc cagaccaatg
    47101 gatattccag ccctttcgat ttgtggatcg tgacaatctg caccagatgt ttatcacttt
    47161 cgagacgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
    47221 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
    47281 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttcagcaa
    47341 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
    47401 gccgataacc atcgaactct tcgactacca catcccacgc atgttcgtca ttgttcagcg
    47461 tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta cgcagggtgt
    47521 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
    47581 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
    47641 cgtcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
    47701 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
    47761 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
    47821 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaaca
    47881 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
    47941 acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attcctggtg
    48001 cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc gccttcatat
    48061 aagtgaagat atccgcaccc cggaatgcat atatggcctg tttcgggtcg ccaatcagca
    48121 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
    48181 cggtatcctg aaattcatca atcattgcca ccgggaatcg cgtacggatc gccgctgcca
    48241 acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
    48301 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
    48361 gggttatcac cagatcacgg atcgacaatg gttctgcaag cagttgttcg atcgcctcaa
    48421 acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctgag
    48481 agaatttttc cagcgactcc ggtaactgat aactgttggt ctcttcttct gcccaggcgc
    48541 tgatcttctc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
    48601 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat
    48661 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
    48721 ctttgataac cggtgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
    48781 gccctttcca ggtttcaaag accaccaggg caatttcacg cggcagcggg tagcagtggc
    48841 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
    48901 gctcaaacag cataccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt
    48961 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
    49021 cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt
    49081 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg
    49141 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
    49201 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
    49261 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
    49321 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
    49381 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
    49441 ttctggctgc cggaaatctg cgacaggata gccatgcctt gcggctcgac caccgcctga
    49501 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
    49561 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
    49621 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
    49681 tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
    49741 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
    49801 cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc ggtacgcaac
    49861 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg
    49921 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
    49981 gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt tcgacaccac
    50041 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc
    50101 tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
    50161 taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc atctcgcgag
    50221 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc
    50281 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
    50341 gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg
    50401 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
    50461 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
    50521 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
    50581 acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac gcgcagattc
    50641 gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat cagcgagaaa
    50701 tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc agctttttgc
    50761 tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc atcgacaaaa
    50821 taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
    50881 gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc aatattgact
    50941 gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    51001 tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt cgccagttca
    51061 tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct aaaaattgcc
    51121 gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga gatcgctaat
    51181 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    51241 tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    51301 aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
    51361 agaactttac gcggcagtgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    51421 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    51481 tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc
    51541 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
    51601 ggtttatcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    51661 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    51721 aattcagcgt tcaccgcatt acgctcacgt tcggcatatt tcttgtcgag caaaggttca
    51781 gcaatagcat cggccaggcg gtctaccgca ccaggcaagg cgtcgttctc aacttccaga
    51841 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    51901 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    51961 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    52021 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    52081 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    52141 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac aaacaacaat
    52201 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg
    52261 gccagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    52321 cagcctatgt ctatatatcc atcagtccgt gactggtgcg catcataaag taagcggata
    52381 gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa
    52441 acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagcct
    52501 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    52561 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    52621 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    52681 catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    52741 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    52801 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    52861 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
    52921 ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat
    52981 gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa
    53041 agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc
    53101 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gcccttccag
    53161 aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca cctgcaaacg
    53221 catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    53281 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    53341 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    53401 cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    53461 ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat
    53521 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    53581 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc
    53641 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
    53701 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    53761 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    53821 gctgatacgt tcttgatcca gacgctgcgc cagttcatca cgcaatagtg acagcggcac
    53881 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    53941 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    54001 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
    54061 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
    54121 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    54181 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gtggggagtt ccagctcgcg
    54241 aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata
    54301 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc
    54361 cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc
    54421 cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    54481 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    54541 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg
    54601 atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    54661 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    54721 agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
    54781 tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag aaaggagata
    54841 aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga catcctgttc
    54901 accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg gcaattcgcg
    54961 atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag caggatcttt
    55021 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    55081 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    55141 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    55201 ctgatagaga ttggcgcggt gccaacgcgg ttgtccgagt tcatgggtat attccaccag
    55261 cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
    55321 tgtttcccac tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc
    55381 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg
    55441 caacagggta aagtcttcgc gatccagcaa ttgcggcagc agagtcatca gtttccagct
    55501 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    55561 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    55621 cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg
    55681 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    55741 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    55801 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    55861 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga
    55921 aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct
    55981 gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga caatcatcac
    56041 catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat tcttcaggga
    56101 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
    56161 acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    56221 accaataagg cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc ggttccagcg
    56281 tactgcgagc actgaactgc gggctgcgtc tgccaggagt gcattttccc ccacgccagc
    56341 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca
    56401 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac cagcagcatc
    56461 agaaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat cctgtcacgc
    56521 tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    56581 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    56641 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    56701 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct tttaccggtt
    56761 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc
    56821 cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat
    56881 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc
    56941 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    57001 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    57061 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    57121 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    57181 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    57241 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    57301 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    57361 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc gttgcgactg
    57421 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc
    57481 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    57541 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    57601 ccgttgcagg acgttgtaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa
    57661 aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    57721 gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt
    57781 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    57841 ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc
    57901 agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt
    57961 agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc
    58021 aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca
    58081 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt
    58141 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct ggcgttggcc
    58201 aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg gcccattcgt
    58261 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc
    58321 acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    58381 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    58441 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    58501 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    58561 cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg
    58621 caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    58681 agccaatcag gaacaagcca gagacagctc ccattgggcg cggtttacga atatacaggt
    58741 tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc
    58801 gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    58861 aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc
    58921 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    58981 tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg cgggcaaaga
    59041 taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga
    59101 cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac
    59161 caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    59221 ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa
    59281 tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa
    59341 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    59401 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    59461 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    59521 tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa
    59581 aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    59641 gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac
    59701 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat
    59761 cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga
    59821 tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg
    59881 tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga
    59941 aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga
    60001 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    60061 gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc ggcaacagaa
    60121 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    60181 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    60241 tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    60301 gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    60361 ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac
    60421 ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac
    60481 gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    60541 caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca
    60601 gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa
    60661 tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt
    60721 tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagag
    60781 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    60841 gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc
    60901 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    60961 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    61021 gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    61081 caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc
    61141 cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    61201 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    61261 atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc gactgagaaa
    61321 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    61381 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    61441 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    61501 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    61561 tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg
    61621 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    61681 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    61741 tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    61801 ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca
    61861 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    61921 gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    61981 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    62041 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    62101 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    62161 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    62221 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    62281 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    62341 aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctg
//
LOCUS       DAFAVF010000009        59201 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFAVF010000009 DAFAVF010000000
VERSION     DAFAVF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.009"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..547)
                     /locus_tag="LGK42_000545"
     CDS             complement(<1..547)
                     /locus_tag="LGK42_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656831.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCT"
     gene            complement(647..1003)
                     /locus_tag="LGK42_000546"
     CDS             complement(647..1003)
                     /locus_tag="LGK42_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBK2656832.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1139..1246)
                     /gene="rprA"
                     /locus_tag="LGK42_000547"
     ncRNA           complement(1139..1246)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="LGK42_000547"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(1432..2544)
                     /gene="ydiK"
                     /locus_tag="LGK42_000548"
     CDS             complement(1432..2544)
                     /gene="ydiK"
                     /locus_tag="LGK42_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBK2656833.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            2933..5989
                     /locus_tag="LGK42_000549"
     CDS             2933..5989
                     /locus_tag="LGK42_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK2656834.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELYAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEEKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            5986..6396
                     /gene="menI"
                     /locus_tag="LGK42_000550"
     CDS             5986..6396
                     /gene="menI"
                     /locus_tag="LGK42_000550"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBK2656835.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            6496..6684
                     /locus_tag="LGK42_000551"
     CDS             6496..6684
                     /locus_tag="LGK42_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBK2656836.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            7233..7601
                     /gene="sufA"
                     /locus_tag="LGK42_000552"
     CDS             7233..7601
                     /gene="sufA"
                     /locus_tag="LGK42_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBK2656837.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            7610..9097
                     /gene="sufB"
                     /locus_tag="LGK42_000553"
     CDS             7610..9097
                     /gene="sufB"
                     /locus_tag="LGK42_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK2656838.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            9107..9853
                     /gene="sufC"
                     /locus_tag="LGK42_000554"
     CDS             9107..9853
                     /gene="sufC"
                     /locus_tag="LGK42_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK2656839.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            9828..11099
                     /gene="sufD"
                     /locus_tag="LGK42_000555"
     CDS             9828..11099
                     /gene="sufD"
                     /locus_tag="LGK42_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK2656840.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            11096..12316
                     /gene="sufS"
                     /locus_tag="LGK42_000556"
     CDS             11096..12316
                     /gene="sufS"
                     /locus_tag="LGK42_000556"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBK2656841.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            12329..12745
                     /gene="sufE"
                     /locus_tag="LGK42_000557"
     CDS             12329..12745
                     /gene="sufE"
                     /locus_tag="LGK42_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBK2656842.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            12894..13898
                     /gene="ldtE"
                     /locus_tag="LGK42_000558"
     CDS             12894..13898
                     /gene="ldtE"
                     /locus_tag="LGK42_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBK2656843.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(13962..14198)
                     /gene="lpp"
                     /locus_tag="LGK42_000559"
     CDS             complement(13962..14198)
                     /gene="lpp"
                     /locus_tag="LGK42_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBK2656844.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(14509..15921)
                     /gene="pykF"
                     /locus_tag="LGK42_000560"
     CDS             complement(14509..15921)
                     /gene="pykF"
                     /locus_tag="LGK42_000560"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBK2656845.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            16478..16687
                     /gene="fumD"
                     /locus_tag="LGK42_000561"
     CDS             16478..16687
                     /gene="fumD"
                     /locus_tag="LGK42_000561"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBK2656846.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            17143..17769
                     /locus_tag="LGK42_000562"
     CDS             17143..17769
                     /locus_tag="LGK42_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBK2656847.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            17790..19892
                     /locus_tag="LGK42_000563"
     CDS             17790..19892
                     /locus_tag="LGK42_000563"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBK2656848.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            19905..20543
                     /locus_tag="LGK42_000564"
     CDS             19905..20543
                     /locus_tag="LGK42_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBK2656849.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            20607..21275
                     /locus_tag="LGK42_000565"
     CDS             20607..21275
                     /locus_tag="LGK42_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBK2656850.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            21272..22057
                     /locus_tag="LGK42_000566"
     CDS             21272..22057
                     /locus_tag="LGK42_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBK2656851.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            22061..22873
                     /locus_tag="LGK42_000567"
     CDS             22061..22873
                     /locus_tag="LGK42_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656852.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(22885..24489)
                     /locus_tag="LGK42_000568"
     CDS             complement(22885..24489)
                     /locus_tag="LGK42_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBK2656853.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(24615..24920)
                     /locus_tag="LGK42_000569"
     CDS             complement(24615..24920)
                     /locus_tag="LGK42_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBK2656854.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(25028..25104)
                     /locus_tag="LGK42_000570"
     tRNA            complement(25028..25104)
                     /locus_tag="LGK42_000570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25068..25070),aa:Val,seq:gac)
     gene            complement(25109..25185)
                     /locus_tag="LGK42_000571"
     tRNA            complement(25109..25185)
                     /locus_tag="LGK42_000571"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25149..25151),aa:Val,seq:gac)
     gene            25493..26749
                     /locus_tag="LGK42_000572"
     CDS             25493..26749
                     /locus_tag="LGK42_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBK2656855.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(26790..28163)
                     /gene="mdtK"
                     /locus_tag="LGK42_000573"
     CDS             complement(26790..28163)
                     /gene="mdtK"
                     /locus_tag="LGK42_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBK2656856.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            28378..29019
                     /gene="ribE"
                     /locus_tag="LGK42_000574"
     CDS             28378..29019
                     /gene="ribE"
                     /locus_tag="LGK42_000574"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBK2656857.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(29059..30207)
                     /gene="cfa"
                     /locus_tag="LGK42_000575"
     CDS             complement(29059..30207)
                     /gene="cfa"
                     /locus_tag="LGK42_000575"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBK2656858.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(30498..31709)
                     /locus_tag="LGK42_000576"
     CDS             complement(30498..31709)
                     /locus_tag="LGK42_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBK2656859.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            31822..32754
                     /locus_tag="LGK42_000577"
     CDS             31822..32754
                     /locus_tag="LGK42_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2656860.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(32751..33776)
                     /gene="purR"
                     /locus_tag="LGK42_000578"
     CDS             complement(32751..33776)
                     /gene="purR"
                     /locus_tag="LGK42_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBK2656861.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            34075..34164
                     /gene="ynhF"
                     /locus_tag="LGK42_000579"
     CDS             34075..34164
                     /gene="ynhF"
                     /locus_tag="LGK42_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBK2656862.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            34330..35499
                     /locus_tag="LGK42_000580"
     CDS             34330..35499
                     /locus_tag="LGK42_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2656863.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(35645..36226)
                     /gene="sodB"
                     /locus_tag="LGK42_000581"
     CDS             complement(35645..36226)
                     /gene="sodB"
                     /locus_tag="LGK42_000581"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBK2656864.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(36354..37169)
                     /gene="mepH"
                     /locus_tag="LGK42_000582"
     CDS             complement(36354..37169)
                     /gene="mepH"
                     /locus_tag="LGK42_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBK2656865.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            37504..37851
                     /gene="grxD"
                     /locus_tag="LGK42_000583"
     CDS             37504..37851
                     /gene="grxD"
                     /locus_tag="LGK42_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBK2656866.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(37902..42517)
                     /locus_tag="LGK42_000584"
                     /pseudo
     CDS             complement(37902..42517)
                     /locus_tag="LGK42_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(42610..43257)
                     /gene="rnt"
                     /locus_tag="LGK42_000585"
     CDS             complement(42610..43257)
                     /gene="rnt"
                     /locus_tag="LGK42_000585"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBK2656867.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(43360..43767)
                     /gene="gloA"
                     /locus_tag="LGK42_000586"
     CDS             complement(43360..43767)
                     /gene="gloA"
                     /locus_tag="LGK42_000586"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK2656868.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(43848..44945)
                     /gene="nemA"
                     /locus_tag="LGK42_000587"
     CDS             complement(43848..44945)
                     /gene="nemA"
                     /locus_tag="LGK42_000587"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBK2656869.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(44982..45581)
                     /gene="nemR"
                     /locus_tag="LGK42_000588"
     CDS             complement(44982..45581)
                     /gene="nemR"
                     /locus_tag="LGK42_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBK2656870.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            45684..45923
                     /locus_tag="LGK42_000589"
     CDS             45684..45923
                     /locus_tag="LGK42_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBK2656871.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            45972..46868
                     /locus_tag="LGK42_000590"
     CDS             45972..46868
                     /locus_tag="LGK42_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBK2656872.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            46949..47470
                     /gene="sodC"
                     /locus_tag="LGK42_000591"
     CDS             46949..47470
                     /gene="sodC"
                     /locus_tag="LGK42_000591"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBK2656873.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(47471..49483)
                     /locus_tag="LGK42_000592"
     CDS             complement(47471..49483)
                     /locus_tag="LGK42_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2656874.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(49483..50340)
                     /locus_tag="LGK42_000593"
     CDS             complement(49483..50340)
                     /locus_tag="LGK42_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2656875.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(50343..50579)
                     /locus_tag="LGK42_000594"
     CDS             complement(50343..50579)
                     /locus_tag="LGK42_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBK2656876.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            50780..51214
                     /gene="slyA"
                     /locus_tag="LGK42_000595"
     CDS             50780..51214
                     /gene="slyA"
                     /locus_tag="LGK42_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBK2656877.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(51261..51728)
                     /gene="slyB"
                     /locus_tag="LGK42_000596"
     CDS             complement(51261..51728)
                     /gene="slyB"
                     /locus_tag="LGK42_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBK2656878.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            52002..53111
                     /gene="anmK"
                     /locus_tag="LGK42_000597"
     CDS             52002..53111
                     /gene="anmK"
                     /locus_tag="LGK42_000597"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBK2656879.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            53209..53538
                     /gene="mliC"
                     /locus_tag="LGK42_000598"
     CDS             53209..53538
                     /gene="mliC"
                     /locus_tag="LGK42_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBK2656880.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            53597..54253
                     /gene="pdxH"
                     /locus_tag="LGK42_000599"
     CDS             53597..54253
                     /gene="pdxH"
                     /locus_tag="LGK42_000599"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBK2656881.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            54382..55656
                     /gene="tyrS"
                     /locus_tag="LGK42_000600"
     CDS             54382..55656
                     /gene="tyrS"
                     /locus_tag="LGK42_000600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK2656882.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            55715..56578
                     /gene="pdxY"
                     /locus_tag="LGK42_000601"
     CDS             55715..56578
                     /gene="pdxY"
                     /locus_tag="LGK42_000601"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBK2656883.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(56622..57227)
                     /gene="gstA"
                     /locus_tag="LGK42_000602"
     CDS             complement(56622..57227)
                     /gene="gstA"
                     /locus_tag="LGK42_000602"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBK2656884.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(57333..58835)
                     /gene="dtpA"
                     /locus_tag="LGK42_000603"
     CDS             complement(57333..58835)
                     /gene="dtpA"
                     /locus_tag="LGK42_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBK2656885.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        15037 a        14438 c        15478 g        14248 t
ORIGIN      
        1 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
       61 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
      121 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
      181 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cctgccagaa
      241 agccaaaaac ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca
      301 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
      361 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
      421 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
      481 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
      541 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
      601 ccgttcatta ttgttgtaac aacgacaatt cctttttgtg gagaaattaa caaagccgct
      661 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
      721 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
      781 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
      841 gcatagccaa cagttgctcg actacacgat caggaatggc gcatttgcca ttttcccact
      901 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
      961 tggcaaaaat atggcgaagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     1021 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     1081 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     1141 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     1201 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtaagc taatagtagg
     1261 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     1321 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaatcaaa
     1381 tatcaattag cgggggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     1441 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     1501 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     1561 aaacagaccg atcatcccga aagcaatcaa accaccaata acaccagaga ggatcaggat
     1621 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     1681 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccag tccagtagag
     1741 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     1801 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     1861 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     1921 tgccgccagc agtacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     1981 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     2041 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     2101 ggtgccaata taagggcgga ctttcgccat gatcgccgtc ccccccatat ccagcaagtt
     2161 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     2221 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     2281 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     2341 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     2401 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     2461 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     2521 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     2581 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     2641 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     2701 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     2761 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     2821 gcaactcgaa atccatagag taaataaaca tcacctttca gtacgctatt gcagccgcct
     2881 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     2941 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     3001 gcaacaaggt tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac
     3061 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     3121 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     3181 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     3241 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     3301 ggccggggtg ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     3361 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     3421 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     3481 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgaattgg cggaaacgct
     3541 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     3601 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     3661 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     3721 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc ccttgcctaa
     3781 agtgcgccgt ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc
     3841 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     3901 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     3961 agagatgctc gggctgaaca ttgtggagtt tgctggtgat gatgaggcgc tgattgatga
     4021 gcgggtaaat gcactctgtg cgcggttgga tgagctgatc gccagtcatc aagcaggtgt
     4081 catcggctgg caggtgtgcc gtgagctggc gggggttgaa cgtatctatg caatgcgcaa
     4141 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     4201 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     4261 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     4321 ccgtccggca ctggatatgt gcgatcctca acaagagatt ttgatgaagc aaatttctga
     4381 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     4441 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttatgcag aactgcgcaa
     4501 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     4561 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     4621 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     4681 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     4741 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt tgttggcgga
     4801 tcgcggcgtt gatccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     4861 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     4921 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     4981 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttc ctgcagctct atcatacccg
     5041 ttatttacgt ccgctgcgcg accacctcgt cgctacggtc gagagctacg cgccgctgat
     5101 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     5161 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     5221 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     5281 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     5341 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagccgg tgttactgcc
     5401 attttcgcca aatggcaaag cccagcatat taaaggtttt cttaatcgtt ttgcgaagac
     5461 ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag ctggggatgc caatggtggg
     5521 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     5581 acgtggcgag tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgagtcaca
     5641 gccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     5701 cgccttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     5761 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagagaaaaa
     5821 ccataaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     5881 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtcaaac gggttgaagg
     5941 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacga
     6001 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttgctggat
     6061 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     6121 acaaagcagc ctttcgggtt gctgcatgga ggcgcatccg tggtgctggc cgaaagtatc
     6181 ggttccgttg ccggttattt atgtactgaa ggtgagcaaa aagtggttgg cctggaaatc
     6241 aatgctaacc acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg caaaccgttg
     6301 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
     6361 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
     6421 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
     6481 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
     6541 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
     6601 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
     6661 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
     6721 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac ccgcctgata
     6781 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
     6841 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
     6901 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
     6961 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
     7021 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
     7081 tgcctgttaa cccacacatc agggtctatg cttattaaaa gcaggcaaaa agcatgtttt
     7141 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
     7201 atacgctgaa agcgatgaag tgaggtaaat cgatggacat gcattcagga acctttaacc
     7261 cacaagattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgtg
     7321 agctggtggc aaagcagccg ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct
     7381 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
     7441 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt attgatggca
     7501 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
     7561 cccagaatga atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa
     7621 tactgaagca actgacgatg tcaaaacatg gaccggcggc ccgctgaatt ataaagaagg
     7681 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
     7741 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
     7801 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
     7861 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
     7921 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
     7981 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
     8041 cattttcgac tctgtttcgg ttgccactac ttatcgcgaa aaactggcgg agcagggaat
     8101 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
     8161 cggcaccgtg gtgccgggga atgacaactt ctttgccgcg cttaatgcgg cggtagcctc
     8221 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
     8281 ttttcgcatt aacgcggaaa aaactgggca gtttgagcgc accattctgg tggccgacga
     8341 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
     8401 acacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
     8461 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
     8521 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
     8581 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
     8641 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
     8701 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
     8761 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
     8821 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
     8881 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
     8941 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
     9001 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
     9061 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
     9121 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
     9181 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
     9241 aacgcttgcc gggcgagaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
     9301 agatttgctt gcgctgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
     9361 gtatccggtg gagattcccg gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
     9421 ggtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
     9481 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
     9541 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
     9601 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
     9661 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
     9721 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
     9781 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
     9841 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
     9901 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
     9961 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagccag tttgtcagca
    10021 ttgcgggaga gatatcccca cagcagcgag atgccttagc gttaacgtta gacgctgtgc
    10081 ggctggtatt tgtcgatgga cgttacgtgt cagcactgag cgatgcgact gaaggcagcg
    10141 ggtatgaagt gagcattaac gacgaccgtc agggggtacc cgacgctatt caggcggaag
    10201 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    10261 gtcaacggcc ggcaaagcca ttgctgttaa tgcatattac ccagggcgtg gcaggtgaag
    10321 aggtcaacac tgcccattac cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg
    10381 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcactt caccggcgca cggttcacta
    10441 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    10501 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    10561 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    10621 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    10681 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    10741 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    10801 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    10861 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    10921 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    10981 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    11041 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    11101 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    11161 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    11221 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    11281 cgctcaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    11341 ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc
    11401 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    11461 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    11521 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    11581 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgctgggaa cagaaaatcc
    11641 gctggcggaa ataatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    11701 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    11761 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    11821 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    11881 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    11941 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    12001 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    12061 tctcactctc tatggcccac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    12121 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    12181 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    12241 ggccatgtat aacacccatg aagaagtgga tcgtctggtg accggactgc aacgtattca
    12301 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    12361 gcgtaatttt ttacgctgcg ccaattggga agagaaatat ctctacatta ttgagctggg
    12421 ccagcgtctg ccagaattac gcgacgaaga ccgaagccca caaaatagca ttcagggctg
    12481 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    12541 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtatttattc tctacgatca
    12601 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    12661 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    12721 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    12781 tgccggatgg tcaatgttta cactgacctg cacaggagtg ttttggtttc aggtgaacat
    12841 aagcgccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    12901 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    12961 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    13021 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcggcaa
    13081 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    13141 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    13201 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    13261 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    13321 ctaacccaac ctggacgcct acggcaggca ttcgtcaacg ttcgctggag cgtggcatta
    13381 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    13441 tcgcgcatgg taatggcgaa tacctcattc atggtaccag tgcgccggac agcgtcggtt
    13501 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    13561 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    13621 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    13681 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    13741 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    13801 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    13861 aaccagagca agggaatatg ttacgcgcga cgcagtagcg gtaaacggca gacaaaaaaa
    13921 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    13981 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    14041 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    14101 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    14161 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    14221 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    14281 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    14341 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaga taaagtgaga
    14401 ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa
    14461 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    14521 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacaac
    14581 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    14641 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    14701 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    14761 tactgcgcga gcagatttac cgccctgagt agcaaccacg atcagcggag catccagttt
    14821 ttcagcagtt tcaacggcac cacggcatac cgcttcggta atgcgcagtt tacggttgtc
    14881 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaaa tggtcgccat
    14941 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    15001 atcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    15061 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    15121 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    15181 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    15241 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    15301 caggtgctca cggatttcga taacgtcaga acgcttacgg ataaaggaag cagcaacaaa
    15361 gtctacgcct tgttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    15421 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    15481 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    15541 atcgtcaacc agtacggtgt tgccaactga caggtcagta gtgaaacctt catacgttac
    15601 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    15661 agctttcaga gaaacgtcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    15721 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    15781 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    15841 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    15901 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    15961 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    16021 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    16081 aacgtgagtg cctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    16141 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt
    16201 ttcaaaaaat atacaacttg ttagacaata aattgttatc aaagatttaa cgttccaggg
    16261 aaaaggttac gcaaaaactt cactgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg
    16321 aggataagtg aaagttcatc cggtgtataa atatatcatc gctcaattgc tacagttaaa
    16381 ataatttatc gcgttatttg tctgattttg tttgattttt gtttaattgg ttagattgat
    16441 atatgctgat agttggcatg ataaataggt ataaagaatg ggaaatcgga caaaagagga
    16501 tgaactgtac cgagaaatgt gcagagttgt cggtaaagtt gtgctggaaa tgcgcgatct
    16561 ggggcaggaa ccaaagcata ttgttatcgc aggcgtatta cgtacagcat tagccaataa
    16621 acgcattcag cgcagtgagc tggaaaagca ggcgatggaa accgtcatta acgcactggt
    16681 aaaatgacca ttcaggatga ggcggtaagc ttcatcctga tattcttgct tcagttgcgg
    16741 tccagttaat cattctaaat aacaactgct gaaattatac gaattgcggt atgtatttta
    16801 atttatagcg ataatgttat ttttattttc tgcaatgaac gtcacaaata attgtcgctt
    16861 aagttaaatt tatttcaaat gataataaat atcagttcac ttataatttc ttgctttgta
    16921 attttaattg ataacgatca atgttaaggg ctcagaataa tcacattatt ttttctccta
    16981 accataaagg attagtttat cggcgttaaa tcttttctca tacactatcg ggttagggaa
    17041 aatatctggt gttggggaca gagtcagcag cgtattagtt gaatgcttaa tatcaggctg
    17101 ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc ggttgatcgt
    17161 ccactattag atattggctt aacgcgatta gaattcttgc gtatatcagg aaaaggcctt
    17221 gctggtctaa caattgcccc tgcgttactg tcacttttgg gttgcaaaca ggaagatatt
    17281 gatagcggca cagtagggtt gataaataca cccaaagggg tgctggtgac ccagcgagcg
    17341 cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca acttcaacga tggctcagta
    17401 gggacattct tctcccgtat caaaatccat cgtaattatt tctttggcga caacggggtt
    17461 ggctctggcg gcggcctgta tggcgatctc aactataccg cggacacctg ccgtcaatgc
    17521 aaagagccgc aatgcatgaa cgtctgcccg attggtgcga ttacctggca gcagaaagaa
    17581 ggctgtatta ccgtcgatca taaacgctgt attggctgta gcgcctgtac tacggcatgc
    17641 ccgtggatga tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg tgtgttatgc
    17701 ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg gaaagatatt
    17761 actgtgtgaa tcttgcaaag gaaaaaatca tggctaacgg ttggacaggt aatatattaa
    17821 gagtcaatct cacgacagga aatattaccc tcgaagattc cagtaagttt aaaagttttg
    17881 tcggtggcat gggcttcggc tacaaaatta tgtatgacga agtaccgcca ggcacgaaac
    17941 ctttcgatga agcgaataaa ttagtctttg ctaccggccc attaactgga tctggtgccc
    18001 cctgtagttc tcgcgtaaat atcacctcac tttctacttt taccaaagga aatttagttg
    18061 tcgatgccca tatgggtggc ttttttgcag cgcaaatgaa attcgctgga tacgacgtca
    18121 ttattatcga agggaaggcg aaatcaccgg tatggctgaa gattaaagat gacaaagtta
    18181 gcctggaaaa agccgatttc ttatggggaa aagggacgcg cgcaacgacg gaagaaattt
    18241 gtcgattgac cagtccggaa acctgtgtgg cggctattgg tcaggctggg gaaaaccttg
    18301 ttcctctctc tggcatgttg aatagccgta accacagcgg cggtgcggga actggcgcaa
    18361 ttatgggttc gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg gtcaacattg
    18421 ccgatcgtca ggagatgaag cgtctcaacg attacatgat gactgaactt attggtgcga
    18481 ataacaacca tgtcgtgcca agtacgccgc aatcgtgggc agagtattca gatcccaagt
    18541 cacgttggac agcacgtaaa gggctgtttt ggggcgcggc tgaaggtggt ccgattgaaa
    18601 cgggggaaat tccgccaggc aatcagaaca cggtcggctt tcgtacctat aaatccgttt
    18661 ttgacttagg tccggcggca gagaaataca cagtaaaaat gagtggctgc cactcttgcc
    18721 cgatccgttg tatgacccaa atgaatattc ctcgggtgaa agagtttggc gtgcccagca
    18781 caggtggtaa cacttgtgta gccaactttg tccataccac catcttcccg aacgggccga
    18841 aagattttga agataaagac gatggtcgtg tgattggtaa cctggtgggt ctgaatttgt
    18901 tcgatgacta cggcctatgg tgtaactacg ggcagttgca tcgcgacttt acctattgtt
    18961 acagcaaagg ggtgttcaag cgtgttctgc ctgctgaaga gtatgcggaa attcgctggg
    19021 atcaactgga agcgggtgac gttaacttca ttaaagattt ttactaccgt ctggcgcatc
    19081 gtgtgggtga gctgagtcac ctggctgatg gttcatatgc catcgcagaa cgctggaatt
    19141 tgggtgaaga gtactggggc tacgcgaaaa ataaactctg gtcgccgttt ggctatccgg
    19201 ttcaccatgc caatgaagcg tcagcgcagg tcggttccat tgttaactgt atgttcaacc
    19261 gtgactgcat gacgcatacc catatcaatt ttattggttc gggtttgcca ttgaaactgc
    19321 agcgtgaagt ggcgaaagag ctttttggtt ctgaagatgc ctacgatgaa accaaaaact
    19381 acacgccaat caatgacgca aaaattaaat atgcaaagtg gtcgctgttg cgggtctgtt
    19441 tgcataacgc cgtcaccctg tgcaactggg tctggccaat gaccgtttcg ccgctgaaaa
    19501 gccgtaatta ccggggcgat ctggcgcttg aagccaaatt cttcaaagcg atcaccggcg
    19561 aagagatgac tcaggaaaaa ttagatttag ctgcagagcg tatctttacg ttgcatcgtg
    19621 cctacacggt aaaactgatg caaaccaaag atatgcgtaa tgaacacgat cttatctgtt
    19681 cctgggtatt cgacaaggat ccgcagatcc cggtctttac tgaaggtact gacaaaatgg
    19741 atcgtgacga tatgcatgct tcgctcacga tgttctacaa agaaatgggc tgggacccac
    19801 agcttggttg tccgacccgc gaaacattgc agcgtctggg gctggaagat attgccgccg
    19861 atctggcagc acacaatcta ctgcctgtgt aaggaatggg tgagatgaac catcgggatg
    19921 aattgccgtt agcgaaagtc agcgaagttg acgaggcgaa gcggcaatgg ttacagggga
    19981 tgcgtcaccc cgttgatacg gtaaccgaac cagaaccggc tgaaatcctg gcagagttca
    20041 ttcgccagca ttctgcggct gggcagctgg tggcgcgcgc cgtgtttctc tcgccaccct
    20101 attcggttgc agaagaggag ctgtcagttt tgctggaaag cattaagcag aacggagact
    20161 acgcggatat cgcttgtatg acgggaagtc aggatgatta ctattactca acgcaggcca
    20221 tgagtgaaaa ctacgcggcg atgtctttgc aagttgtgga gcaggacatt tgtcgcgcca
    20281 ttgcacacgc cgttcgtttt gagtgccaga catatcctcg tccttacaaa gtcgccatgc
    20341 tgatgcaagc gccatattac ttccaggaag cgcagattga agccgcgatc gccgcgatgg
    20401 atgttgcacc tgaatatgcc gatatacggc aggtggagtc atcgacggcg gtgttgtact
    20461 tgtttagcga gcgatttatg acctatggca aggcttatgg cttatgcgaa tggttcgagg
    20521 tagagcagtt tcagaacccc tgaaatttca gggagatgtc atggataggg tggacagttg
    20581 cagccactgc attaggggat aatcagatgt ccttcactcg acgcaaattt gttctgggga
    20641 tgggaacagt aatctttttt actggttctg cgtcttctct gttagcgaac acgaggcaag
    20701 aaaaggaagt tcggtatgcc atgattcatg acgagtcacg gtgcaatggc tgtaatattt
    20761 gcgcccgcgc atgccgtaaa actaaccacg tccccgctca gggaagccgt ttatcgatag
    20821 cacacattcc agtcaccgat aacgataacg aaacgcagta tcactttttt cgccagtcat
    20881 gtcagcactg cgaagatgct ccgtgcatcg acgtatgccc gacaggggcg tcgtggcgtg
    20941 atgagcaggg gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt tattgcattg
    21001 gcgcttgtcc gtaccaggtt cgctacctta accctgtcac taaagtggca gataaatgtg
    21061 atttctgcgc tgagtcccga ctggcaaaag gctttccgcc catttgcgtc agcgcctgcc
    21121 cggaacacgc attgatcttt ggtcgcgaag atagcccaga aattcaggcg tggttgcagc
    21181 aaaataaata ctatcaatat cagctacctg gtgccgggaa accgcatctg tatcgccggt
    21241 tcggtcaaca tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg ctgaacagtt
    21301 tcagagccag ttagcgaact atgtgccaca gttcactccc gaattttggc cggtgtggtt
    21361 gatcattgcc ggagtattgc tggttgggat gtggttggtg ctggggctgc atgccttgct
    21421 tcgtgctcgt ggcgtgaaga aatcagtcac cgattatggt gagaagattt atctttactg
    21481 caaagcggtc agattatggc actggtcgaa tgcgttactc tttgtattgt tgctggcaag
    21541 tgggctgata aatcactttg cgctggtggg cgcaactgcg gttaaaagtc tggttgcggt
    21601 gcatgaagtt tgcggatttt tgttactcgc atgctggctc ggctttgtgc tgatcaatgc
    21661 cgttgggggt aatggtcacc actatcgcat tcgtcgtcag gggtggctgg agcgagcggc
    21721 aaaacaaacg cgattttatt tgtttggcat tatgcagggg gaagaacatc ctttcccggc
    21781 aacaacccag tctaaattta atcccttaca gcaggtcgcc tatgttggtg ttatgtatgg
    21841 attgctgccg ttgttactat tgacggggct gctgtgtctc tatccgcaag ccgtgggaga
    21901 tgtgtttcct ggcgtaagat actggttatt gcaggcgcat tttgctctgg catttataag
    21961 cctctttttt atcttcggtc atctttatct ttgcaccacg gggcgtacgc cacacgaaac
    22021 ctttaaaagc atggtcgatg gctatcaccg gcactaacga atgatcatta cacgagcaga
    22081 cctgcgagaa tggcgtatcg gagcggtgat gtatcgctgg ttcctgcgcc atttcccacg
    22141 cggcggtagt tatgccgata ttcatcatgc actgattgag gaaggatata cggactgggc
    22201 ggagagtctg gttgagtatg cgtggaaaaa atggctggcg gatgaaaact tcgcgcatca
    22261 ggaagtttcc tctatgcaaa aactggccac agaccccgga gagataccat tttgcagtca
    22321 gtttgcccgc agcgacgatc atgctcgcat cggctgttgt gaggataacg cccgaatcgc
    22381 aaccgctggg tatgccgcgc aaattgccag tatgggttat agcgtgcgga ttggaagtgt
    22441 cggctttaac agtcacattg gcagctccgg tgaacgcgca cgggttgctg taaccggtaa
    22501 ttcttcgcgg ataagcagcg ctggagatag tagtcggatt gcgaataccg gaatgcgagt
    22561 acgcgtttgt acattgggtg aacggtgtca tgtcgccagt aatggtgacc tggtgcaaat
    22621 cgccagcttt ggcgcaaacg ccagaattgc caacagcggc gacaatgtac acatcattgc
    22681 cagcggtgaa aattcaacgg tagtgagcac gggcgtggtc gactcaatta ttctcgggcc
    22741 agggggaagc gccgcactgg catatcatga cggcgaaaga gttcgcttcg ccgtcgccat
    22801 tgaaggtgaa aacaatattc gaaccggagt ccgctatcgt cttaatgaac agcatcagtt
    22861 tgttgagtgc tgatagcttt acctttaatc aaacgaaaga cgccgacgag cacgggatgc
    22921 aggctttacg accgcccgag ccatcgcctc accaatttct gcacatgccg taagtccctg
    22981 aacgaaaggc tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc gtccacgaat
    23041 atcttcgggt tgctgtaacg tataatcttc gccaacatca gggatttcat cccctgtttt
    23101 ctgtaattgc tcgcgtagcc cagggaaagg aatatctttc actttaagcg gacgctgtcc
    23161 gcgggcatca ataaaaacgt caaacgagta gctgttgtct tccgttttca ggacggtgcg
    23221 cgactcatta atttccattt tgtagtcttc accgagggcg agaatatgaa tgattcccgc
    23281 ttcacgtaag gcaagtaggc gacgaataga ctctgaaggg attgccgcat aattatcgat
    23341 gaaaaccctg gcaaggcctt tactgaaccg tttatggtcg tgttcattca gatgaggaac
    23401 aatttcctgt acggcttcat gcaggcgcag aatgacataa cgccagggaa cagtatgttt
    23461 ttctcgttta ttgcgttcga cttcctggag atttttttct gcccagtcaa atgggtcgcg
    23521 ttgtttgcgc tcggcaaacc aggcttgagc aaaggaatcg acattcaggc tctctaaggc
    23581 tatgcgttgg ctccagtctg gatcagcaaa cttgatttcc tctactatca atctaaatac
    23641 ccgatccaaa aggccctctt cacctttttg aatctcagca tttaatgcct gatcggtgac
    23701 gatgtgtaag ggctcgtagg gaatagggca atagaaatcg gcttcgggta aaatacccgt
    23761 gcgcgacatc aacgtaatat ttagcttttc acttgcgtta tcgcggttaa aaacgacgtg
    23821 ttgtttatca tcttcaatga acgaaccatg ctgaatagcc actgccattg ccgcatccag
    23881 tccgctcaag gatgttccca taatacccac gttacacgca tcgacctttg cttccatcag
    23941 gcctgaccac ggactgggaa aatacgttct ggttgcttct tcttcatcag gccagacgtg
    24001 acccgtggca atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat ttgtagcgag
    24061 catgacgcca gcatttgtaa tttgcagatc ggtaacctgg catgattcat aaacagccac
    24121 tgcaaatttt tgctgtcgtg cctggtctac taatcgtaaa aattgatcgc ggaaatattc
    24181 gcccagcaga attcgcggta aaaactgacg atcgtgcaag gtttcttttt taacgccata
    24241 acgctggaga tggctggctt cttgcttttg tagccattcg agatacgtac aattaatcgg
    24301 cggtatttca atactggcaa tatttgccag catcattttt gagttttcct catcactgta
    24361 tggcatcccg acaccggcct cgtcagcctg ctcgaagata gaaatagaaa gtggagtttg
    24421 ttgctgtaga agcgagaata aggtgtagat ccccgtaggc ccggcaccca caatagcaat
    24481 ttttttcatt gttacttacc tgtacgcatt ctttcgaatt tagtgttatc agataagttt
    24541 ggttgagatt tttagaattt gagaaatttc agacagggat gaaaagggcg ctaaccgccc
    24601 ttgattaaat tctgtcaggc gagttttgcc tgattgattt gactcagtgg ctcattgaca
    24661 tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt taagtcgggc agtatgtttt
    24721 tccagatagg caagggcgct tttttcatca gtaaaaagat agatcccacc ggcttcgtgg
    24781 ttcttttcac tttctgtcca taccttccac agaaaaccag gttcctgatt aatcgactca
    24841 gcaagcggtt taagctgctc agccattgcg tcgccaaatg ggccattaaa agcaaaatga
    24901 agttgtaaca aagttgccat agcgattctc cttctgataa cgcatacgaa gacagtgtaa
    24961 atcttttaac gtattggata cagagaagaa acagagggaa aaaggagaag accagattaa
    25021 gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc aaggtggtgc
    25081 tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa ttggactcga accaacgacc
    25141 cccaccatgt caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt tgtaggtgac
    25201 aacggggacg aatattagcg gcagagtggg aaggtggcaa gaggcaaaac gtaattttct
    25261 gcgctatttc gaccgtttgc agagctttta agcaaattgg ctatattttg ttgatttgca
    25321 agggcgattt ttattcagga tcgcatttac atctgataca acccaataat tcgtgtatca
    25381 gagtttaaac agagttgttt gccaccatct caaatctgtc gtggataaat cctccgtagc
    25441 ctttacactt aggtagcaat aaaggcacac agatgcagca aaggaataaa tcatgggatc
    25501 tgatgcgaaa aacttgatga gcgacgggaa tgtgcaaatt gttaagaccg gcgaggtcat
    25561 tggcgcgacg caacttactg aaggcgagtt gattgttgaa gctggcggaa gagccgaaaa
    25621 taccgtggtc acgggggctg gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac
    25681 acagtacggt aacaatggca cgctatcggt cagcgacggt gccattgcca cagatattgt
    25741 tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct acggttaatg gccgccatcc
    25801 cgaaggtgaa ttcagcgttg atcagggtta tgcctgcggt ttgttgctgg aaaatggcgg
    25861 taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc attctcgatc aagagggcgg
    25921 cctgttggtt aatgggacaa cctcagcggt cgtggtagat gaaggtggtg aattgttggt
    25981 gtatccaggt ggggaagcca gcaattgtga gattaatcag ggcggcgttt ttatgctggc
    26041 cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg aataatctcg gtggtgaaga
    26101 ctctgacact attgttgaga atggatccat ttatcgtctg gggacggatg gccttcagct
    26161 ctacagttcc ggtaagacgc aaaacctgtc cgtgaatgtg ggtggtcggg ctgaagtgca
    26221 tgccggtacg ctggaaaatg cggtaataca aggtggaaca gtgatcctgt tgtcacccac
    26281 cagcgcggac gaaaattttg tcgtagagga agatcgtgca ccggttgaac tgaccgggag
    26341 tgttgcatta ctggacggcg cttcaatgat tattggctat ggcgcagatc tgcaacaatc
    26401 aacgattact gtacagcagg gcggtgtgtt gattctcgac ggcagtacgg taaaaggtga
    26461 cggtgtcact tttattgttg gtaacatcaa tctgaatggc ggaaaactgt ggctgatcac
    26521 tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg gagcgatttg
    26581 cctgcaaacc agtgcgaaag aaatctcacc tgacttcatc aatgtgaaag gggaagttac
    26641 cggggatata cacgttgaga taacagatgc cagtcggcaa actctgtgca acgctctgaa
    26701 attacagcca gacgaagacg ggattggcgc aacgctccag cctgcgtaaa aacacaggcg
    26761 caacccgtga aggttgcgcc ttgtctttat tagcgggatg ctcgttgcag aatgataact
    26821 gacggcagac gttgcaggaa ccgcatacgc agcatcatca taatggctgc cgacgtcagg
    26881 ccaataataa agcctatcca gaagcctgct ggccccatag gttcaacgac cagatcggtc
    26941 agtgccagaa tatagccgct tggcaagccc agcacccagt aagccgtaaa ggtgatatag
    27001 aaaatggaac gcgtatcttt ataaccacgc aaaatcccac tgccaatcac ctggattgag
    27061 tcagaaatct gatataccgc cgccagcaac atcaaatgcg cagccagcgt tacaacctcg
    27121 ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa tatggccgtc
    27181 agggttgcca tacagacacc caccataagc ccggtccgcg cagcggtttg cgcatccagc
    27241 gttgagccct gacccagacg ataacctacg cggatagtta ccgctgccgc cagcgacatt
    27301 ggaagcacaa acatcagtga actaaagttc agggcaatct ggtgtcctgc gacatcaaca
    27361 ataccgagcg gagacactaa cagagccacg acggcaaaca gtgtcacttc aaagaatagt
    27421 gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca tcaccgcagg atcgggtttc
    27481 gcggtgcctt tttcgttacg aatatcgcgc atggagcggg cgcgtttaat gtaagaaacc
    27541 atggcaagga acatgaccca atacaccgcc gcagtagcca cgccacaacc aacgccaccg
    27601 agctcaggca taccgaaatg accataaata aagatatagt tcaccggaat gttcaccagc
    27661 aggccgataa agcccattac catacccggc ttggtttttg ccagaccttc acattggtta
    27721 cgggcaacct ggaagaacag atagcccggc gcgccccaca acaacgcacg cagataaccc
    27781 acggctttgt ccgccagagc cggatcgatg ttttccatgg agcggataat gtaacctgca
    27841 ttccacagca caagcataat gagaacggaa acaaaacctg ccagccagaa accttgtcgc
    27901 acctgatgcg caatgcgctc acgtcgaccg gaaccattta attgcgcgat aaccggcgtt
    27961 aatgccagca gcagtccgtg accaaagagg atcgccggaa gccagataga agtaccgata
    28021 gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca tcacggtatc gacaaaaccc
    28081 atcgcagttt gggcgatttg cgcgagaatc accgggattg ctaatgctaa taacagacgc
    28141 gcttcactga tatacttctg cacgtgaaca ccttttattt gtagttatat gagagactaa
    28201 aaagccgcct gaatgggcag caaaaagaag aagcagggga aattcagtct attgtagcga
    28261 ggatttatta attctccagt gaaaaaatag tcattcttac cccttcactg gtaagggcgt
    28321 ttgccacctg ctattgtgct gaacagaatg tcttaactga tttcaggagt tgtaagtatg
    28381 tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt
    28441 cgtacgcatg tggtggagtt acccgaccac atgctggacg gcctggaaac aggtgcttcc
    28501 gtggcgcata acggttgctg cctgaccgtg acggaaatta acggcaacca tgtcagtttt
    28561 gacctgatga aagaaacgtt acgcattacc aatcttggcg atttaaaagt gggggattgg
    28621 gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg gcggacactt aatgtcaggt
    28681 catattatga ccaccgctga agtggcgaaa atattaacct cggaaaataa tcgccagatc
    28741 tggtttaaag tccaggatag tcagttgatg aaatatatcc tgtacaaagg atttattggc
    28801 atcgacggta ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg cgtccattta
    28861 attccggaaa ctctggaacg cacgactctt gggaagaaaa aactcggcgc acgcgtcaac
    28921 attgaaattg atccacaaac tcaggcagtg gtagatacgg tagaacgtgt gctggcggca
    28981 cgagaaaatg ccatgaatca accaggtaca gaagcctgat ctgaaatcgc ccccggagag
    29041 ggggcgatag aatagccttt agcgagcgac tcgaaggccg ttttcaacac cgcgtgagaa
    29101 cacgacctgc cagagctgaa tatcacgggc gcggaaagca cctgcacagg cattcagata
    29161 ataggtaaac attcgtttaa agcgttcact gtagttatcc gcaatttctg gccatgcggc
    29221 gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa tcagcaccga agttatgcca
    29281 gtcttccatc acgaagtggg gttcgctgga ttgagcaatc tggcgtacag agggcaggca
    29341 accgttcgga aaaatatatt tattaatcca gggatcaaca ttcagatcgg tttttttcga
    29401 accgatagta tggagcagga atataccttc cggtttcaaa ttacgatcca ccaccgcaaa
    29461 ataggtatcg taatttttcg gtccgacgtg ctcgaacatc cccacagaaa caatacgatc
    29521 aaactggtcg ttcaggtcac gataatcttg cagcaaaatg gtgacatcca ggccttcaca
    29581 gcgttcctga gccatttttt gctgttcggc agaaatggtg acgccgacca cgcttacgtc
    29641 atagttagat gccatgtagt gcgccagtcc gccccagccg cagccaatat ccagtacgcg
    29701 catccctggt tttaactgca atttttcaca aatcattttg agcttcgcct gctgggcaga
    29761 ttccagatta tcggcatctt tccagtaagc gcaggaatat tgcatgaagg gatcaagcat
    29821 gcggctgaac aagtcattac ccaaatcgta atgctccttg ccgactatcc aggcacgttt
    29881 tttactctgc agattgaaga gacgagcgct ggcaatacgc agcgtgtctt tgaaatgatg
    29941 ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc
    30001 acattcccac cagccatcca tataactttc gcctaacccc aaagagcctt cttgcagaac
    30061 gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc ggggcagaac cgttaatggc
    30121 tataccggca cggctaagta attcgttggc gatacggtac cagttgtcat ccggtacact
    30181 gacttcttct atacacgatg aactcatagt ttctccatca ctggtcggtg atcagaacct
    30241 taaaacagcg tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc
    30301 tataatccga cgtagaacag agggaaagga ggaacccttg ccgaaatgac catccatagt
    30361 gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt atcgtagctt taacaactcg
    30421 aaattagtat aggccgctaa tttcaatcat acaacaaaat tttgttagca gcctaagtat
    30481 aagtatatcg atatagatca gtgtgattcg ctatgagcga cttcggcatt gccatgattc
    30541 tggcagtcaa cttcttcaca acgttgcatc atgtaaccca gagcgaccag cactactgtt
    30601 gataacatca cgctggtggt ggtgagcaat ggcgtgctga tactgatcag ccaggaaact
    30661 accagacttg cgaggaagca cagacccagt tgtagagtgt tctgcaacgc tgcggcgcga
    30721 ccagttgcgt gtgggaaggg acgcagcgcc tgggcgacaa caatcgggta gatcgcgcca
    30781 ttggcaatcg ccatcacaca gaatgggatc aggatttcga ccagcgacac atggctaatg
    30841 aagcctgcag cccaggtcgc aatgacgctg actgcaaaca gcaccagcaa ccacggtaat
    30901 aactgcttgc cttgccattt ctgcagcgcg gcgcgacagc cataaccacc aatcagaaac
    30961 gcgatagttt gcgggacata acttaaacca ataactgccg ggctgtagcc catttcacta
    31021 aggatgaacg gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc cgcgtatatc
    31081 agcacgttgc cgcgataggt tttagaacgt agcaggtcgg taaaggtcag accatcctga
    31141 ctattgttac gggccttcgt cgtgggtttg agccagaaaa taggcagaat cagcaccacg
    31201 gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa aatggaccag cagccagctt
    31261 cctaacagag gagccagggc cggagataga cccaccagcg gcatgatggt cgcaaaaatt
    31321 cggttaactt tctgtgaagg ataataatcc gtcactaacg cttgccagat aaccgccgcg
    31381 gcgcagacac ccacagcctg tacaaaacgc aataccagca gcgtagcggc gttttctacc
    31441 cacagcatcc ccagactacc taacgcaaaa attgtcaggc cgattaataa caccggttta
    31501 cgaccataac ggtcggagag tggcccccac agaagctggg ctgcggcaaa accggcaagg
    31561 aacagactaa ggctggcact gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg
    31621 gcgaaagcag gcagatacat atcggttgcc agaaaaccga gtacgctcaa acccgccagc
    31681 cagactaaaa atcttttccc aggttgcatg tatattctct aaatctctca attaaacacc
    31741 gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg taatatttga tgactggttt
    31801 caaaaatttt gcaggcagac tatgtggtca gaatattcac tcgaagttgt tgatgcggta
    31861 gcgcgtaatg gtagttttag cgctgcggca caggagctgc atcgcgttcc ttctgcggtc
    31921 agctataccg tgcgtcagct ggaagagtgg ctggcggtgc cgctctttga acgacggcac
    31981 cgtgatgtgg agctgaccgc tgctggagcg tggtttctca aagaagggcg ctctgttgtc
    32041 aaaaaaatgc agatcacccg ccagcaatgt cagcagatag cgaacggctg gcgcggtcag
    32101 ttagctatcg cagtggataa tattgtcagg ccagaacgta cacggcagat gatcgttgat
    32161 ttttatcgcc attttgatga tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc
    32221 tgggatgcgc tttccgacgg gcgcgtggaa ctggcgattg gcgcaacacg cgcgattccg
    32281 gtaggcggtc gttatgcctt ccgggatatg gggatgctaa gctggagttg cgttgttgcc
    32341 agccaccacc cgctggcgtt gatggatggc ccattcagcg atgatacgtt gcgcaactgg
    32401 ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta aacgtattac ctggttgctg
    32461 gataaccaaa aaagagttgt cgtgcctgac tgggaatcat cggcaacctg tatctcggca
    32521 ggattatgca tagggatggt gccaacacat ttcgccaaac cgtggcttaa tgaggggaag
    32581 tgggtagcac ttgaattgga aaaccccttc ccggattcgg catgttgtct gacatggcag
    32641 caaaatgata tgtcgcctgc gttaacctgg ctgcttgagt atctcggcga tagtgagacg
    32701 ctgaataagg agtggctgcg ggagccggaa gagactcccg caacgggtga ttaacgacga
    32761 tagtcgcgga acgggccgtc agccacggag cggcgttcaa tcaagcgcgg atgcacttca
    32821 atggactgcg gttcttcacg tttgttgacg atacgatcca acagcatgtt gaacgctgtt
    32881 tcacccagcg aatcttttgg ctggtggatc gtggtcagcg ccggcgtaaa atagcgggcg
    32941 ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg ggacgcgcag acccatttca
    33001 tcagcagcac aaagtgcgcc cattgccatg atatcgccac cacagaagac ggcagtaggg
    33061 cgatgcggct gcgacagtat ttgctgcatg gcgcgataac cagattcggg ttcaaagtca
    33121 ccctgaacaa tccagctttc cggcaccttg atcatcgctt cttccatcgc cttcataaaa
    33181 ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc cagggatgac gccgatttcg
    33241 cggtgaccgc gttcaatcag ataacgcccg gccatgtagc cgccttcgaa cgcgttatca
    33301 atgaccgcat cggtgaagtc agcttttgct tcaccccagt ccatcaccac cattgggata
    33361 tggcgatact cttccagcat cgccagcaac ggctctgggt actcagaaca catcaccagc
    33421 agaccatcga cgcgtttttg cgccatcatc gacagataag cccgctgttt ctcaagattg
    33481 ttccacgcat tacccagaat cagggtgtaa cctttctgga agcaattttt ttcaaccgct
    33541 tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc gatagacttg
    33601 gtgtggttaa ccttcaggct acgcgccacc gcgctagggg agtagtgtaa ttctttaatc
    33661 gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt gattacgtgt
    33721 gacacagttg tagtggaaac gtttgctcgt ttcgctacat cttttattgt tgccattcca
    33781 tttcactcca gaccctaact tcagctcctg caaaatcgca aggtaaacgt ttgcctttac
    33841 acaccttacg cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg agcgtcagga
    33901 aggggtcaat ggccagtacg caaatagtgt aagcgtggaa ttttggccga tcttgacgaa
    33961 aaggggaagt ggtaaaaaca cttatcatta taaatgacgg gagatttttt catcacagtg
    34021 tgtaaaaatg cgtaatatca ctttttgtgt ggtaataaaa aaggagaaaa cttgatgagc
    34081 accgacctta aattttcact ggtaacaacg attatcgtcc tcggtttgat cgtagccgtg
    34141 ggtttgactg ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc ctcgcatctc
    34201 cttcctgttt gttagcaaaa tggcaaaagt gttttctctt tttgttattt tcattttttt
    34261 gtgattgctg tcatgctttc gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg
    34321 agataaggca tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc gtttggtatc
    34381 gggacaacgg agttctcgcc aatgggtttg ttgcccgtca ttgcgcgcgg tgtggatgtc
    34441 tcgattcccg ctgccggaat gttaatcagt gcctatgcag ttggcgtaat ggttggcgcg
    34501 ccgctgatga cgcttctact ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg
    34561 gcaattttca cgctcggcaa cgtactttcc gccatcgcgc cggattatat gaccctgatg
    34621 ctttcacgca ttttgaccag cctgaatcac ggagcatttt ttggtttggg ttcagtcgtg
    34681 gccgcaagcg tggtgccaaa acataaacag gccagcgcag ttgccactat gtttatgggg
    34741 ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct ggttgggtga aaccatcggc
    34801 tggcggatgt catttctggc aacggcgggg ctgggagtga tttcaatggt aagtctgttc
    34861 ttctcattac ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga gctggcggta
    34921 ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg tactgggagc tggtgcaatg
    34981 tttactctct acacctatat ctctccggta ctacaaagta ttacccacgc aacaccagtg
    35041 ttcgtcacgg caatgctggt gctgattggt gtcggattct ctatcggtaa ctatctcggc
    35101 ggcaaactgg cagatcgttc agttaacggc acgttgaaag gctttttgtt gttgttgatg
    35161 gtgattatgc tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc tattagcatg
    35221 gtggtgtggg gcgcagcaac ctttgcggtc gtaccgccgt tacagatgcg cgtgatgcgt
    35281 gtcgccagtg aagcgccggg tctgtcttca tcagtcaata ttggtgcctt taatcttgga
    35341 aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg ctgggctggg atacagcttt
    35401 gtgccggtga tgggagcgat tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc
    35461 agaaaacaac ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa cgcagaagga
    35521 taaccttctg cgttacagtt gctgacaacg tgcgatttat cttgccgaat gcgctgttaa
    35581 cgcctcatcc gcgttggcat ggtaagcgaa gcgcatcagg caatgctgca tttgccatca
    35641 gttattatgc agcgagattt ttcgctacga attcccagtt caccagcgcc cagaagtgct
    35701 ccagatagcc aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc cagacatcaa
    35761 cggtcagcag cggagtcgca tcggtggtca gcggagtacc cgcgttagag gttgaaacga
    35821 tagccagttt gccatcgctg tttttcacca gccaggtcca gccagaacca aagtttttga
    35881 tcgctgcatc agtaaactgc gctttgaaat cggcaaagct gccaaaagat gcggcgatag
    35941 cttcagcgac ttttccagtc ggttcgccac cggcgttcgg tgccaggcag ttccagtaga
    36001 aagtatggtt ccagacctga gctgcgttgt tgaatacgcc accttcagag ctgcgaataa
    36061 tctcttccag tgatttacct tcaaacgcgg tacctttaat caggttgttc aggttagtga
    36121 cataagtctg atgatgcttg ccgtagtgat actcgatagt ttccgcagaa atgtgcggtg
    36181 ccagagcatc tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt
    36241 attattaatt tgcatacgta caatagcctt attgtgcgta tggatagggt agcaaattaa
    36301 aaggtgagac aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa gttttagcga
    36361 agtgtttttg gggtcattac ccgacgagcg ccaacatagt ggcgctgcca gtagtcttca
    36421 ctgagagaag tgatttgaat ttcctgacct gtgcgcggtg actgaataaa tttgccgttg
    36481 ccgacataca cgccgacatg atcggctgtg ccgcgtccct gagtacggaa aaagaccagg
    36541 tcgccgtttt tcagttcact acgttcgatt ggcgctgcat cacgcaggtg atacatttca
    36601 ttcgccgtac gcggaatacg aattttcacc aaatctttat aagcgtaata aaccaggccg
    36661 ctgcaatcaa aaccggtacg cggtgagctg ccaccccaac gatatggctt accaatttgc
    36721 tgcatcagtt tattcattgc cactttcgta gctttttgca ccttcgcttt gtgcgcgtcg
    36781 gcaagagttc ctgacgcggc atttttgact ttcacacaat gcgatttata gcccttacgt
    36841 ttggtacact tttcggtaaa gctggctgag gccgttttat tgacggcgtg tttgctgcga
    36901 gttttggcgt ttttagaagc ggtaatggaa gattttttaa cggaagaagc ggttttgctt
    36961 ttggtcgtac ttttactggc ggcttttttc gctgttttct gggtcttttt ggtggttgcc
    37021 gtgcttttct ttttatctgc ttttttggtg atatgagtgg tagcagaact ctccctggct
    37081 tgctttgaag catgggccat aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc
    37141 gtgatcgaaa tacggtttat ccgcgccact gagcgtccct ctgttaaggc aggtctatca
    37201 tgcctgcgtc tgatttacaa aacttgatga ttctaatgca tagaagaaga gaaagttaat
    37261 agcttttatt gttaaaaaat acgtttcgtt ggcgagcgca aaaaaatgca gcagtgaaag
    37321 ctaaaaatgg ttataaatac gccagtttgc cgcgcatcag agaagaaacg aatatttgaa
    37381 atgcaacaaa tagagcaaag ggataaaata gcaaaagcgt gatgaaaatg ttattttccg
    37441 atgccgcact gacccgctac aatgtcagat tactgccgta aagaagtttg aggaagcaag
    37501 acaatgagca ccactatcga aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg
    37561 tacatgaaag gttcaccgaa actgccgagc tgcggtttct ctgcccaggc agtccaggcg
    37621 cttgccgcat gtggcgaacg ttttgcctat gttgatattc tgcagaatcc ggacattcgt
    37681 gcggaactgc cgaaatatgc taactggccg accttcccgc aactgtgggt tgacggcgag
    37741 ctggtcggcg gttgtgatat cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg
    37801 atcaaagaaa ctgccgctaa atacaagtct gaagagccgg acgcggaata atccgtcctg
    37861 caatactgac ggatgcgaac gggcatccgt cagtcctttc tctatcccca atccagccct
    37921 tgtggcgaac ttgaaaatcc cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc
    37981 ggtagatcat tcacttctgt tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta
    38041 gtcagcgcgt ggaaaacctc tggggcgagt aattcctctt tttcctgcca gaccagcatt
    38101 tttttgccac cttgcgccag atagagtaac aatttgccgc cagaaacgac caccagcgca
    38161 cccgcccgac gcgttggaac cagcgttgcc ggatgtgcag gccagggcag aagatttccc
    38221 cacacattag ccggatcgtt ggcagagagc gccactggtg tatagtggcg cgtttgcgtg
    38281 gcttgtgtcg ccagatcgcg caatcggtca atagtcagac gttcagcgaa ttgcgcgcca
    38341 cccagacctt ctacaaaacg acctcgcata attcgcccgg agtcttccat acttcgacaa
    38401 agcgtttgca tcgatggaaa cccgccaggg atattttcgg ctatcaccgc ctgacgactg
    38461 atgatgccgt agcggtcgag catattttcc gccagcgcca gcatcctttc ggtatcgttt
    38521 agtggctcca cctgcaataa cgaccagcgt ccagccagat ttggtgtgtt gtaagatacc
    38581 agcggcgaga cgggttgcgc atagacagga cgtccacgac gagcccggtg actgcggcga
    38641 gttgaggtgc gtgcgttgga actgctgcgg gtgagggcgc gtaacggtgc ccaaatgtcg
    38701 ctggtgatga cgccttgcca gaccagcgcc cataacgcct cttgcagggc agagagatca
    38761 accgattcgc cgattttgtc gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct
    38821 ccgtcagcca gaacagcgac tatcgcttgt tgcagcgccg aacgattcgc ctgatccgct
    38881 tcggcgggag tgaacgattc tgcagcatat tcctgtagat gcagtgccac caggccgtca
    38941 tcttcaccca gtttttttgc cccgaccaga taactgcacc ggttgccagt aattcatcga
    39001 gcatttctga cgaatagtcg cgtacgcggg caggcaggat ctggctttcc cagagtgagg
    39061 cgggtaaacc gactccggca agctgttcga tcacccgcat cacgccatct acgccctcat
    39121 aaacgcctgg cgatgttgag gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc
    39181 cctgacgttc cagcagcaat cgcgcatagg tcgtggctgc aacgggacgc gtcgcttctc
    39241 tggcggcttg cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc acccagatgt
    39301 cttgttgcag attcatcacc agaccctgtt cacgcagttg ctgaagctgc tcttcgacaa
    39361 tggcaatacc gagactaaac tcatgagcca gttgttcagc cgtgaccaga gcatgagccc
    39421 ggagatagcg cagaaagagg tcgcgaagcg ggtaactgac tctatgtaaa taaatctctg
    39481 gcaatgactc tggtagtcgt acgccgaggg catcacgcag cctggcggca tcatccatac
    39541 aggcaagacg ctcctgtccg ctaatcatcg ctgggaagat tcgttttact gcaagaagat
    39601 tttccagata cgacgcaacc tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa
    39661 cggtcattgg ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc
    39721 tgccaggagc cagtcgttgc aactcttctt ccacctggcg aatgacctgc gggtcgagta
    39781 attcccccgg atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta
    39841 cggatgcccg gcgctctgcc agcggggcgt cgctctggta cataaattcc gcgacatagc
    39901 cgaacaataa acttgtggcg aaaggcgagg gcgtagtggt cgttacatcg gatatttgaa
    39961 tttcgccacc gttcaggcga cgcatcaaac gttccagtgc gggaagatca taaacatctt
    40021 gcagacattc gcgcagggtt tcgagaatga ccggaaaatc cggatatccc tgagcgattt
    40081 ccagcaactg actggcgcgc agccgttgtt gccaaagcgg ggtgcgatgg cccggagtgc
    40141 gccccggcat taataatgcc cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg
    40201 agctgcctac cgcctcgcgg acaatttgca gcaacttttc tggttcaaac aaaaaaatcg
    40261 cggcgtcggg caatttacca tcagtgtcag gaatgcgcgc aacaatgccg tcatcactgg
    40321 cgaccacaga cgcgtcagcg ccccataggg catgtattcg cccggtaatc gccagcgccc
    40381 agggttcatg cacccgtctt ccatagggag agtgcaaaat aatacgccag tcgccaattt
    40441 catcacggca ccgttcgagg accagatggc gactccccgg aacgatgccc gtcgcgttgc
    40501 gttgctcgtc aatcaacccc tgaatattgg cgttcgtatt ttcttctgcc agccacgggg
    40561 gaatagtccc ggaaaagaac gcgccatccg ccagcaaatg aagaaaatcg ccgatcatct
    40621 cgcctaattc agccggacgt ccgttacctt caccacgcca gaaggggagc cgggcagaac
    40681 gacccggagc aggagtcaca atcacctgat cgcgggtgat ttgctggatc cgccatgagg
    40741 tagcgccgag agtgataatg tcgttcaccc gcgactcata taccatctcc tcatccagtt
    40801 cacccacccg ccgcgaaccg gccttttctt caccttcggg taataacacg ctatacatgc
    40861 cacgatccgg aatggtgccg ccgctggtaa ccgccaacaa ttgagcgcca ggtcgggcgg
    40921 tcaatatccc ggtctccctg ttccagacca gtttggggcg aaaagcagaa aaatcgccag
    40981 agggatagcg cccggaaagc atatccagcg tggcgtcaaa aacacgtctt ggcagatctt
    41041 tccacggtgc ggcacggcgt acgcgggagt accattcgtc tacctgtaat gcctccatcg
    41101 ccgcggcggc aacggtttgc tgcgcaagga cgtcgagtgg attatgcggc ggtgtcaggt
    41161 tttccagcct gcctgcgaac atacactcta caatgactgc ggaatcgact aagtcacgcc
    41221 gggtacgagg gaaaaacagc cctttagata caccgccaac ctgatgtccg gcgcgaccaa
    41281 tgcgttgtaa cccactggca acagaaagcg gcgttgccac ctgaatcacc agatccactg
    41341 cgcccatatc aatccccagt tcgagactgg aggttgcgac cacgcagcgt aactcacccg
    41401 atttcagcgc ctgctcggtg attgctcgtt gttctttaga gacggagccg tggtgtgagc
    41461 gggcaataaa aacgtcgcta ctttgtacac ggttagaggt tgcgccggag gtcgactcga
    41521 aatgggccgc atcaacggcg atagacgggg aacgctgtaa gcgtgcggcg taaagctcat
    41581 ttaatcgtgc cgtcagtttt tccgccagcc cacgtgaatt agtaaagaca atggtcgagc
    41641 gatggcgcaa cacttcatca aggatacccg tttcaatata tggccagatg gagccttccc
    41701 ggccggcatg gctgtcttcg ccggtgctgc tggcgaccga tgagacatca tccatattgg
    41761 cgaccggtac gacaattcgt atctgcggat ggcgcattgc gggcgggttg actaccgtaa
    41821 ccgggcgatc gccaccaaga aatgctgcca catcgctggc tgagcgcaca gtggcagaaa
    41881 ggccaattcg ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga cttaacgcca
    41941 gatgcgcacc acgtttactg cccgctaccg cgtggacttc atcaataatt accgtttcga
    42001 cgccgcgtag cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg
    42061 tgatcagaat atccggcgga ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc
    42121 cagtacggat ccctacgcga agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg
    42181 caataccctt caacgggagc tgcaagttgc gctgaacgtc ggtgcccaga gcttttatcg
    42241 gtgaaatata gaggatgcgt gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc
    42301 cttcgcggaa gagccgatcg agggcgtaga gaaatgctgc cagcgttttc ccggagccgg
    42361 tcggtgcaat caccagcgca tgttcgcttc gcgccgccac atgccaggtt tgcgactgga
    42421 cagcggtcgg ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt
    42481 ccggcaggag cgatgaaggg tctggattat ctgccattaa cgctattccc cgtgaatatc
    42541 atgcaggatg cgtggaatag cctcactata gatgcctgaa tcattgcatg tcatcaggca
    42601 tcgactcgat tacacctctt cggcggcagg tagcggccag cctcccagac gtttccagcg
    42661 gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc
    42721 ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc tttgacaata cggtttgtcc
    42781 gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga acgggttacg
    42841 tttcagtgag gcgcgttctg cggcggccat cataaagctg tgatcaaaat tggcattgtg
    42901 cgccaccata atggcgcggt tacagccgct cgctttaata cctttacgta caactttaaa
    42961 aatttcgtgc agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat
    43021 gccgttgaag gcgagggcct ctggctgcaa atttgcgccg acaaatggtt cgacgtggaa
    43081 atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc attttcaggg tgatggcggc
    43141 aatctcaagc agcgcatcgg ttttggcgtt aaatccggct gtttcaacat cgatcacaac
    43201 aggataaaaa ccacgaaaac ggtcgcacag accggtaagt tgagcgttat cggacatctg
    43261 attctcttaa tacggataaa ttgcagcgcg cattatgaca aatattactt gtcgatgcag
    43321 taaagatgcg ggcgcgatga gttcacgccc ggcaggagat tagttgccca gaccgcgacc
    43381 ggcgtctttc tcttcgatta actcaatttt gtaaccgtcc ggatcttcca caaacgcgat
    43441 aaccgtagta ccgcctttta ccggacccgc ttcacgggtc acgttacccc cgttttgacg
    43501 gattttttcg cacgcttcag cggcgttatc tacgctaagc gcgatgtgac cataagcagt
    43561 gccgagttcg tatttatcca cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt
    43621 ttccgggccg tagccaacaa acgccagtga gtatttgtat tccgggtttt cgctggtacg
    43681 cagcagtttc atgcccagca ctttggtata aaaatcgatg gagcgttgca gatcgccaac
    43741 gcgcagcatg gtatgaagaa gacgcatctt tttatcctca aaatggctaa cagattcaaa
    43801 atgttgtttt agtatagcgg cggctttacg ccgctcgcaa tgttggatta caacgtcggg
    43861 taatcggtat agccttccgc gccgccaccg tagaaacttt cggcacgctg tgggttaagc
    43921 tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg caatccagtc acgaccaaat
    43981 gccaccgcat caattaaccc tttgccgatc agcgtttcag ctttttctac tgtgtatgca
    44041 cctgcgccga taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta
    44101 tacggttcac cccccgccca atctggttct gacatatgca gataagcaat gccgcgttta
    44161 cccagttgtt caatcagata cagtgcatcg gcttcttcat tcgggccgtt atccgtgttc
    44221 tggaaagtac cgattggcga aatgcgaatg ccaatgcgat cggcacccca ttcttcaatc
    44281 ccggcatcga ccacttccag taccagacgt gcgcgatttt ccacgctgcc gccgtactga
    44341 tcggtacgat ggtttgaaga aggagaaagg aactgatgca gcaaataacc gtgagcagag
    44401 tggagctcta ccagatcaaa accggcttca cgcgcgttag caatggcctg acggaaatca
    44461 ttgacgatac ctggaatctc ttccagttca agcgcacgcg gcatggatgt ttcaacacgg
    44521 atcgcctgac cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc
    44581 gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac
    44641 agctgcacgg ccatatgacc attttcagca tgaacgccag cggtgatttt tttccatgcg
    44701 gcaatttgct ccggactatg gatgccaggc gcacctgcat atccttttgc ctgggcagaa
    44761 atttgcgtgg cttcactaat aatcaaaccg gcactggcac gttggcgata gtattccgcc
    44821 atcaacgggg taggaatgtc acccggttca atactgcgca gacgcgtcag cggtgccata
    44881 aaaatacggt ttgccgccgt gatcgcgccc actttcagtg gggaatacag tttttcagat
    44941 gacataaagg ctcccggtcg tctggtgata aaaaatgcct gctaaacggc aggcgtcgca
    45001 ataatgtttt ttacatgggc cagcgcgttt tccagtggct cgaaactgcg cgaaattttg
    45061 gcctgcagat tcgcacccag ccatagtgcg taaagcactt gtgcctgttg tagcggttcg
    45121 ccacaaaagg ttaaacaatg gttctcacgg ccattttcca gcgcctgaga gagcagggcg
    45181 atcacgccgc gagcaccttt atccatcgcg ctgcgcatat cttctgacag atcgcacact
    45241 tcggcagaga gttttactgt caggcaacca ctgatggttc catgttggca aaactggttc
    45301 agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc
    45361 aactcagtca gtcgctggtg atatgcggcg taatgacgct caagcatggc aacgccaaac
    45421 gcttctttag agcgaaagta gtgatagaag gaccctttcg gcacttcagc ggtttttagt
    45481 aattcgctta gccccatccc ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc
    45541 aggagatgtt cgcgagtatc atgttcggtg tgtttgttca tgcgttggag tgtagtagac
    45601 cagtcggtct agtgcaagca aagttgctac tttggacatt aacagcttca ataatggtaa
    45661 taatcttatc tgaggagggc gaagtggcgg agcaattaga gttctttccc gtccagagcc
    45721 cgtgccgggg aatttgccag tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc
    45781 gggatgagcg ttttaactgg aataaaatga gcgatggcga aaaacaagag gtgctgcgcc
    45841 tttgtcggca gcgattaatg cgcaaattac gcgcaaataa gccagcgtca tcagacgaac
    45901 cggagcaacc atcactcttt tgaacctaat cttgcgtata ctcctctcat atctgataag
    45961 aggaagcgat tatggttcag cgtattacta ttgcgccgca aggcccggag ttttcccgtt
    46021 ttgtgatggg ctactggcga ttgatggact ggaatatgtc cgcccgccag ctggtcagtt
    46081 ttattgaaga gcatctggat ctcggcgtga ccaccgtgga ccatgctgat atttatggtg
    46141 gctatcagtg cgaagcggcg tttggcgagg cactgaaact ggcacctcac ctgcgtgaac
    46201 ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg
    46261 gtcattacat cactgaccgc gatcacatca ttaagagcgc cgaacagtcg ctaattaatc
    46321 tcgcgaccga tcatctggat ctgctgttaa tccaccgacc agacccgtta atggatgccg
    46381 atgaagtggc ggacgcgttc aagcatctgc atcagagcgg caaagtgcgt cattttggcg
    46441 tatcgaactt tacgcctgcg caatttgccc tgttgcaatc acgtctgccg tttacccttg
    46501 ccactaatca ggtggaaata tccccggtgc atcagccgtt actgctggat ggcacgctcg
    46561 accaactaca acaactgcgt gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc
    46621 tgtttaatga tgattatttc cagcagctgc gtgatgaact ggctgtggtg gcagaggagt
    46681 taaacgcggg ctcgattgaa caggtggttt acgcctgggt attacgttta ccatcgcagc
    46741 cgctgccaat tatcggttca ggtaaaattg agcgcgtacg ggcagctgtc gaagcagaaa
    46801 cactgaaaat gacccgtcaa caatggtttc gtatccgtaa agcggcactg gggtacgacg
    46861 taccgtaagc acttttagga atagccgccg ttcaaaaatg tgtcactggt ttacacttat
    46921 tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt agtctggcta ttctggcgct
    46981 ggttgttgca accggcgcac aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc
    47041 gcaaggggta gggcagtcaa ttggtagcgt caccattact gaaaccgata aaggtctgga
    47101 gttttcgccc gatctgaaag cattaccccc cggtgaacat ggcttccata ttcatgccaa
    47161 aggaagctgc cagccagcca ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg
    47221 gcatcttgat ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg
    47281 cgatctgcct gcactggtcg tcaataatga cggcaaagct accgatgccg tcatcgcgcc
    47341 tcgtctgaaa tcactggatg aaatcaaaga caaagcgctg atggtccacg ttggcggcga
    47401 taatatgtcc gatcaaccta aaccgctggg cggtggcggt gaacgctatg cctgtggtgt
    47461 aattaagtaa ttacgaggcc agcgtacctt gcggtggtgc ttgctcaagt tgcgaaagcg
    47521 agcagtacag ccgccagaca attgccgcca gctcacgggc ggcaggttga tgatgacggg
    47581 caaggctgtc gcaaatccgc tgtaattctt caagtgtggc cgccagtgat ttttgctgaa
    47641 cgccacgctc actcatcact ccccgcaaca gtgaaataca gttatcccgt actcgcgata
    47701 acggatcgga acgcgattcc cagtcgcgca attgccagac aacatgagaa cagttcagca
    47761 gcactacacc ccagcgtaat aaccaacggc gagccagcgc atcctggctg ttactcaact
    47821 gactgacgtg atgataagtg agcgattcaa attcgctttc actcagtgtt ggatggcggc
    47881 ttagctgatc gacaaaatcc cggcgcagcg cgcgaatatg gcggcggctt ttacgagcat
    47941 ccgatcctgg acgcagaatg gcgaacgcta gccaagccag cgcaacgcca acgatttttg
    48001 ccagattatc gttaagaaaa tcagcaaaat catacaccgg gggattagtg acagcgataa
    48061 acgaacccat aaaaacaatc agttgccccc acagtgcggc aaatttgggc atctgcaatt
    48121 taagaagctg cattgtcgcc agcagtggaa agagaaacag taaaaattgc cacagatcgc
    48181 taatctggac catcaggcca aatttgacca caaagctgaa tagcgaaagt aacaccagcg
    48241 tgcgcatcag cagtgacaac gacttaaacg gtgctgcgac ggcggagtag agtacgcagc
    48301 taattgctgc cagcgttaat gcattggcac cggcatccca ttgcgatgca atactccatg
    48361 cgccaatcat catcaacgta caaaatgtac gcagcccgct ccacatagct tcggcgttgt
    48421 cggtatgacg agccagagcg gatgtccgtg cgagtctggc atgatcatct acaccgcttt
    48481 gcagacgatg taattcctga ctactttgca gataaaggcg gcaaaaataa cgtagtcgct
    48541 gccagaaggc gacgtgccgg tagtcggcga cgttggtcgg gcgtagcggg gcgataatac
    48601 gtgcgacggt gtaagcatct gtttgcgaac tggcgagcgc cgtcagcaat tgttcgagaa
    48661 tttctcgcgt ggcacctggc ggtgagggcc agttgagcaa catacgtcgc aggctggaga
    48721 tgacactggt catacgtaat tgctggtgga gtagcgcatt aaggcgcgcg ttttgctggc
    48781 gaaaacgata gtggctccag aaagcctgga tacgcagcaa attcatggtc agtatctgcc
    48841 caatcacccc ctcatgtgct gcacgaatgg catcgtttgt ttcaggctgc cagagtaaac
    48901 tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat
    48961 cggaactgct aggcaggatc atcatcatca ttccgccgca caaaataccg actatcacct
    49021 cgcaaacgcg cgcctgagcg atatcccaca gctggctggc ctcagtaata ttaaccatcg
    49081 gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa tgcatacgcg acgttattcg
    49141 tgaagtgcgc acaggcccag gtacaaaagc caagccacgc cgacatgctc aatagaaaaa
    49201 accacggctc attgagcgta tgcccggcaa gaagcagtgc cgcaatggct cccagtaaac
    49261 tgccagcgat gcgtccgagg cttttgctga tgacaccgcc aacggtggga aagctaacca
    49321 ctgcagccga ggtcatcgcc caatagggtt catccagatt caaataatag gcaaccgtca
    49381 gcgccagaca catggcaatg gtattgcgta acgcataacg ccattgcgcc agcgtggcct
    49441 tgaaccaggg caaattgcgc aaggaccatg acgatgcgtt cattattgac ctacggcaat
    49501 gctacaggtg gtgcccgaca ccagcgtgac atcgcctgga actttatcca gcgcaaagcg
    49561 gacgggaacg cgttgggcga gacgaaccca ggggacgtta ggtttcacat ccggaataag
    49621 gcttgagtca ctttcaacgc tttgatcata aatcgcccga ccgatactgg aaacgtgacc
    49681 ccgtaacgtt ttgttatcac tatagagggt aatttgtgct ggtgcacctt cgcggatatg
    49741 gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac gagtggctgt cgacaagggc
    49801 aaacagcggt ttcccggtgt cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca
    49861 accactcacc ggggcgcgaa tttccgtttg cgccagttgc cattgtgtct gtttcagcgt
    49921 ggcctgtgcg gcattaacac tggcctgcat cgctttaaca ttgaggttag cggtatcgag
    49981 ttcttcggca gaaataaaat tttgtgagag atgacgacga cgattcgctt cgttattggc
    50041 ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt gcgttcagct cggcgatctg
    50101 aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt
    50161 tatattcagc tcaacaatac gtcccgacac ctgtggggtg atagaaacct tttctgcgcg
    50221 tattttcccg tcgcgtgtcc acggagattg catgtaataa ttccacagcc accatccggc
    50281 aagaaccgca actatcgcta caatgattgt tgagaaatac ttaattgttt taattgacat
    50341 agttaccacg caatcagtat tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa
    50401 tccattaaca agggatgcca gatgtcaccg gcgtagatcc agccacgaag caggcggtgt
    50461 acgacaagcc agatgacgaa tccaaacgcg aaggctttga aaaacggagg aaaatagacg
    50521 gacgcaccga acaccagatc ttgtaagggc aatcctgttg cattgagcat aaacttcaca
    50581 aagtagattc ctttacgacc gaatatattg cgttagatta ataaatattc tttaagtgcg
    50641 aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc agcaatacat
    50701 ttgttttagc aatacaattg ctgcacacta ttctaaaagc cgcataatat cttagcaagc
    50761 taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac ggttggtgcg
    50821 catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac aaacccattg
    50881 ggttacgtta cacaatatcc atcagttacc tccagaccag tcgcaaattc aactggcaaa
    50941 agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg aagaaaaagg
    51001 gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta aactgacgga
    51061 aaaggcagag ccactgatca gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat
    51121 attacatggc atctccgcag aggaactgga gcaactgatt acgctcatcg caaaacttga
    51181 gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac
    51241 ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc attgctggcc
    51301 agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac caccatgatg
    51361 gtattaccat cgtctttacg aatttccagc tcgacaccct gcgttttgtt cattgcactc
    51421 tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc cagagaacgc
    51481 ccggttccgc caccaacagt attccccagg aaaccaccaa gaacagcacc gccaattgca
    51541 ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt aacgatggtg
    51601 ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc ccctgacagg
    51661 gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac caatacgcgt
    51721 ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc ctcatggtca
    51781 aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag caaatcataa
    51841 tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga tttgaagata
    51901 atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga aaagtttatg
    51961 tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta
    52021 ttggcgttat gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaatagatg
    52081 aacaccgggt cgcacagctg gcaagcttga gctggccgat cccggtgtct ctgaaacagg
    52141 ctgtactgga tatttgccag ggccagcagc ttacactttc gcagtttgga cagcttgata
    52201 ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc
    52261 aggcgagaga tatagttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg
    52321 gcgtggcacc acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa
    52381 ttacggttgt cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc
    52441 tggtacctgc gttccatcat gccttgctgg ctcactcaac cgagcgacga atggtgctca
    52501 atattggcgg catcgccaat ctgtcactgc tcattcctgg gcagccggtt ggcggctacg
    52561 ataccggtcc tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt
    52621 acgataaaga tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata
    52681 tgctcagcga cccatatttc tcgcaacctg caccgaaaag caccggacgc gaatacttta
    52741 actacggctg gctggagcgc catttacgcc attttccggg tgttgatccc cgtgatgttc
    52801 aggccacact ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg
    52861 gctgcgaacg attgatggta tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc
    52921 tggcggcatt actgccaggc acagaagtca ccaccaccga tgccgttggc attagtggcg
    52981 atgacatgga agcattggct ttcgcctggc ttgcctggcg gacgctggcg ggattaccag
    53041 gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt actgggggct attttccctg
    53101 ctaacccgtg atatcagagt taactgaatt tttcttaccg cgtctgccgt tacacttgga
    53161 acgttaggga gggcgtattg ccctccagac caggaaagtc ttcgggaaat gtctatgaaa
    53221 aaactgttac tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt caatcaactg
    53281 gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg atgaaaaacc gttgacggtc
    53341 aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc
    53401 aaacagggca tttcagcgtc tggtgcacgt tacactgacg gaatctatgt tttctggtcg
    53461 aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg tattgaataa ctgtcagtta
    53521 caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca
    53581 atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg
    53641 tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc gccgatccat taaccctttt
    53701 tgaacgttgg ctctctcagg cgtgtgaagc caaactggca gaccctaccg cgatggtggt
    53761 cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc gttttactca aacattacga
    53821 cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa
    53881 taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat
    53941 cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg
    54001 tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat
    54061 ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc
    54121 gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga
    54181 gcatcgcctg catgaccgct ttttgtacca gcgagaaaat gatgcgtgga agattgatcg
    54241 tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc
    54301 actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc
    54361 gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg
    54421 gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg
    54481 atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt
    54541 ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc
    54601 ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc
    54661 gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc gttcctcgat
    54721 ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg
    54781 aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac
    54841 aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt
    54901 tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg
    54961 ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt
    55021 cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcactaa agcagatggc
    55081 accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg
    55141 tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag
    55201 ttcttcacct ttatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc
    55261 ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt
    55321 gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt
    55381 gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg
    55441 gaaaagggcg cagacctgat gcaggcactg gtcgattctg aactgcaacc ttcccgtggt
    55501 caggcacgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat
    55561 cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc
    55621 ggtaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg
    55681 agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca
    55741 gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccga tgcgccgtct
    55801 gggcgcgaac gtctggccac tgaacaccgt tcaattttct aatcacaccc aatacggcaa
    55861 atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc
    55921 cattgataaa ttacacacct gtgatgccgt gttaagtggc tatctgggat cggcggagca
    55981 gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata
    56041 tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc
    56101 agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga
    56161 gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg
    56221 cgaactcatt gcgcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta
    56281 cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagcctggc atatcagccg
    56341 tccgctggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt
    56401 actgctggtg aaactgcttc agggggcaac gctgcaggag gcgctggaac atgtgaccgc
    56461 tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt
    56521 ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa
    56581 tatctcattt cggcccacaa cgacctgtgg gccgatggat attactttaa gccttccgct
    56641 gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc tgcaatgtgc
    56701 tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag
    56761 gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc
    56821 acatattgca gcttcttctc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta
    56881 tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat
    56941 tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg
    57001 ctgtcggcaa gatactgcat aatcgctacg ccttccgtca gcaaagtacc gtcatccagc
    57061 agcaatgcag gcacctgccc cttagggtta acgacaaagt aatcgtcacc gttttcgaga
    57121 cgttttttca ttaaatccac actgacgagg gtaaaatcct ttccgctctc acgcagggtg
    57181 atatgggaag cgagagagca ggcacccggt ttgtagaaca atttcatcgg taactccttt
    57241 tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa
    57301 gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagcagcttt cgccgctttg
    57361 tctgcagcgt catcctgcgt catacggtgc agtttaggcg cggtcagcag catcagtact
    57421 gcaataatgg cagtagcgac accaatctgc aagaatacgc gaccataaac ttccagtgac
    57481 atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc
    57541 aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc
    57601 ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc
    57661 tgcaggccat agcttgcgac cagccagctt acagacacga taccagcgtc agacgcgaat
    57721 ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac
    57781 ttggttggca tcggcagggt atcgcccatc ttgttataga tagcggccag aatcggacta
    57841 ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc
    57901 agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg
    57961 tacagcacga agaagataat ggcttcgagc atcaggatga aggcaacgat cattttacga
    58021 cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accgaaggca
    58081 acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg
    58141 atcagtgcca caacaccaat aatggtcagc agcaggttac ggtagttgat aggctcgaag
    58201 tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg
    58261 atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc
    58321 cacggcgtag caatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg
    58381 aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg
    58441 ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg
    58501 gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc
    58561 ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga
    58621 ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc
    58681 agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc
    58741 cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg
    58801 acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat
    58861 attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg
    58921 gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa
    58981 tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta
    59041 aatttttcaa aaaacgtaac ggcagatttt ttcactgcac tgagaggggg gtgatggctt
    59101 aacggcgata tgttctgcta tttagcgttt ttactagtga gatagtccag tttctgaaaa
    59161 atagccagtg taatgttttg taggtcaata tttgttgcgt g
//
LOCUS       DAFAVF010000010        58827 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFAVF010000010 DAFAVF010000000
VERSION     DAFAVF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.010"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..2202
                     /locus_tag="LGK42_000604"
     CDS             <1..2202
                     /locus_tag="LGK42_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBK2656886.1"
                     /translation="QDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDV
                     SDSNADVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGST
                     LSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSV
                     ALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPL
                     HPQYELYAGLANADDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWS
                     NPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQN
                     NTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDN
                     GGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDS
                     FSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSS
                     TLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSD
                     SVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYN
                     YIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSV
                     VKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2199..3287
                     /locus_tag="LGK42_000605"
     CDS             2199..3287
                     /locus_tag="LGK42_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBK2656887.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3375..4673)
                     /gene="exuT"
                     /locus_tag="LGK42_000606"
     CDS             complement(3375..4673)
                     /gene="exuT"
                     /locus_tag="LGK42_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBK2656888.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5156..6568
                     /gene="uxaC"
                     /locus_tag="LGK42_000607"
     CDS             5156..6568
                     /gene="uxaC"
                     /locus_tag="LGK42_000607"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK2656889.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6583..8070
                     /gene="uxaA"
                     /locus_tag="LGK42_000608"
     CDS             6583..8070
                     /gene="uxaA"
                     /locus_tag="LGK42_000608"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK2656890.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8153..8704
                     /locus_tag="LGK42_000609"
     CDS             8153..8704
                     /locus_tag="LGK42_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBK2656891.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(8709..9953)
                     /gene="sstT"
                     /locus_tag="LGK42_000610"
     CDS             complement(8709..9953)
                     /gene="sstT"
                     /locus_tag="LGK42_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK2656892.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10569..11534)
                     /gene="alx"
                     /locus_tag="LGK42_000611"
     CDS             complement(10569..11534)
                     /gene="alx"
                     /locus_tag="LGK42_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBK2656893.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11817..12803)
                     /locus_tag="LGK42_000612"
     CDS             complement(11817..12803)
                     /locus_tag="LGK42_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2656894.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(12882..13565)
                     /locus_tag="LGK42_000613"
     CDS             complement(12882..13565)
                     /locus_tag="LGK42_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBK2656895.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(13642..14145)
                     /locus_tag="LGK42_000614"
     CDS             complement(13642..14145)
                     /locus_tag="LGK42_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBK2656896.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14230..15366
                     /gene="rlmG"
                     /locus_tag="LGK42_000615"
     CDS             14230..15366
                     /gene="rlmG"
                     /locus_tag="LGK42_000615"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBK2656897.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            15557..15640
                     /locus_tag="LGK42_000616"
                     /pseudo
     CDS             15557..15640
                     /locus_tag="LGK42_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            15637..16053
                     /gene="higA"
                     /locus_tag="LGK42_000617"
     CDS             15637..16053
                     /gene="higA"
                     /locus_tag="LGK42_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBK2656898.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16098..18116)
                     /gene="fadH"
                     /locus_tag="LGK42_000618"
     CDS             complement(16098..18116)
                     /gene="fadH"
                     /locus_tag="LGK42_000618"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBK2656899.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18442..20793)
                     /gene="ygjK"
                     /locus_tag="LGK42_000619"
     CDS             complement(18442..20793)
                     /gene="ygjK"
                     /locus_tag="LGK42_000619"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK2656900.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20810..21880)
                     /gene="ygjJ"
                     /locus_tag="LGK42_000620"
     CDS             complement(20810..21880)
                     /gene="ygjJ"
                     /locus_tag="LGK42_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBK2656901.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(22014..23447)
                     /locus_tag="LGK42_000621"
     CDS             complement(22014..23447)
                     /locus_tag="LGK42_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2656902.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23510..23959)
                     /locus_tag="LGK42_000622"
     CDS             complement(23510..23959)
                     /locus_tag="LGK42_000622"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBK2656903.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23956..27048)
                     /gene="ebgA"
                     /locus_tag="LGK42_000623"
     CDS             complement(23956..27048)
                     /gene="ebgA"
                     /locus_tag="LGK42_000623"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBK2656904.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(27232..28215)
                     /gene="ebgR"
                     /locus_tag="LGK42_000624"
     CDS             complement(27232..28215)
                     /gene="ebgR"
                     /locus_tag="LGK42_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBK2656905.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFICGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28434..28766
                     /locus_tag="LGK42_000625"
     CDS             28434..28766
                     /locus_tag="LGK42_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBK2656906.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(28808..30187)
                     /gene="ygjG"
                     /locus_tag="LGK42_000626"
     CDS             complement(28808..30187)
                     /gene="ygjG"
                     /locus_tag="LGK42_000626"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBK2656907.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30605..32125
                     /gene="aer"
                     /locus_tag="LGK42_000627"
     CDS             30605..32125
                     /gene="aer"
                     /locus_tag="LGK42_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBK2656908.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(32279..32902)
                     /gene="nfeR"
                     /locus_tag="LGK42_000628"
                     /gene_synonym="yqjI"
     CDS             complement(32279..32902)
                     /gene="nfeR"
                     /locus_tag="LGK42_000628"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBK2656909.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33190..33954
                     /gene="nfeF"
                     /locus_tag="LGK42_000629"
     CDS             33190..33954
                     /gene="nfeF"
                     /locus_tag="LGK42_000629"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBK2656910.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(34008..34083)
                     /locus_tag="LGK42_000630"
     tRNA            complement(34008..34083)
                     /locus_tag="LGK42_000630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34048..34050),aa:Ile,seq:cat)
     gene            34208..34714
                     /gene="mug"
                     /locus_tag="LGK42_000631"
     CDS             34208..34714
                     /gene="mug"
                     /locus_tag="LGK42_000631"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBK2656911.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(34793..36634)
                     /gene="rpoD"
                     /locus_tag="LGK42_000632"
     CDS             complement(34793..36634)
                     /gene="rpoD"
                     /locus_tag="LGK42_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK2656912.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(36829..38574)
                     /gene="dnaG"
                     /locus_tag="LGK42_000633"
     CDS             complement(36829..38574)
                     /gene="dnaG"
                     /locus_tag="LGK42_000633"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK2656913.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(38685..38900)
                     /gene="rpsU"
                     /locus_tag="LGK42_000634"
     CDS             complement(38685..38900)
                     /gene="rpsU"
                     /locus_tag="LGK42_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK2656914.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            39137..40150
                     /gene="tsaD"
                     /locus_tag="LGK42_000635"
     CDS             39137..40150
                     /gene="tsaD"
                     /locus_tag="LGK42_000635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK2656915.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(40193..41656)
                     /gene="ttdT"
                     /locus_tag="LGK42_000636"
     CDS             complement(40193..41656)
                     /gene="ttdT"
                     /locus_tag="LGK42_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBK2656916.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(41704..42309)
                     /gene="ttdB"
                     /locus_tag="LGK42_000637"
     CDS             complement(41704..42309)
                     /gene="ttdB"
                     /locus_tag="LGK42_000637"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBK2656917.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(42306..43217)
                     /gene="ttdA"
                     /locus_tag="LGK42_000638"
     CDS             complement(42306..43217)
                     /gene="ttdA"
                     /locus_tag="LGK42_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBK2656918.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            43424..44356
                     /gene="ttdR"
                     /locus_tag="LGK42_000639"
                     /gene_synonym="dan"
     CDS             43424..44356
                     /gene="ttdR"
                     /locus_tag="LGK42_000639"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBK2656919.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(44369..44986)
                     /gene="plsY"
                     /locus_tag="LGK42_000640"
     CDS             complement(44369..44986)
                     /gene="plsY"
                     /locus_tag="LGK42_000640"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK2656920.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            45091..45459
                     /gene="folB"
                     /locus_tag="LGK42_000641"
     CDS             45091..45459
                     /gene="folB"
                     /locus_tag="LGK42_000641"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBK2656921.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            45549..46370
                     /gene="bacA"
                     /locus_tag="LGK42_000642"
     CDS             45549..46370
                     /gene="bacA"
                     /locus_tag="LGK42_000642"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK2656922.1"
                     /translation="MSDMYSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(46533..47771)
                     /gene="cca"
                     /locus_tag="LGK42_000643"
     CDS             complement(46533..47771)
                     /gene="cca"
                     /locus_tag="LGK42_000643"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBK2656923.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
     gene            complement(47835..48455)
                     /locus_tag="LGK42_000644"
     CDS             complement(47835..48455)
                     /locus_tag="LGK42_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBK2656924.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            48697..49998
                     /gene="ygiF"
                     /locus_tag="LGK42_000645"
     CDS             48697..49998
                     /gene="ygiF"
                     /locus_tag="LGK42_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBK2656925.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            50021..52861
                     /gene="glnE"
                     /locus_tag="LGK42_000646"
     CDS             50021..52861
                     /gene="glnE"
                     /locus_tag="LGK42_000646"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBK2656926.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            52909..54342
                     /gene="hldE"
                     /locus_tag="LGK42_000647"
     CDS             52909..54342
                     /gene="hldE"
                     /locus_tag="LGK42_000647"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBK2656927.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(54462..54521)
                     /gene="ibsE"
                     /locus_tag="LGK42_000648"
     CDS             complement(54462..54521)
                     /gene="ibsE"
                     /locus_tag="LGK42_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBK2656928.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(54838..54897)
                     /locus_tag="LGK42_000649"
     CDS             complement(54838..54897)
                     /locus_tag="LGK42_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2656929.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(55136..56797)
                     /locus_tag="LGK42_000650"
     CDS             complement(55136..56797)
                     /locus_tag="LGK42_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK2656930.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(56824..57453)
                     /locus_tag="LGK42_000651"
     CDS             complement(56824..57453)
                     /locus_tag="LGK42_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBK2656931.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            57722..57922
                     /gene="glgS"
                     /locus_tag="LGK42_000652"
     CDS             57722..57922
                     /gene="glgS"
                     /locus_tag="LGK42_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBK2656932.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(57965..58771)
                     /locus_tag="LGK42_000653"
     CDS             complement(57965..58771)
                     /locus_tag="LGK42_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656933.1"
                     /translation="MMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIAAPECIDIK
                     GAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLPDEYIALE
                     FKGMSGAGAIEVDKNLTFRIRGLNNIHILDCFVNVALEPADGVVDFGKINSRTIKNTS
                     VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIFHKDGTATE
                     FNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
BASE COUNT        14043 a        15030 c        15680 g        14074 t
ORIGIN      
        1 caagacatca atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt
       61 gcgctgggaa taacagaaaa tagttatatt ggtgcacact ggaatttcaa cggttatgat
      121 gcagatgatg tcagtgacag taatgctgac gtcagcgatc tctattatcg ttatgatttt
      181 ttacgtcgtt attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa
      241 ggcgggaact ttacctttaa ctttctgcca ctcggtgcaa ttgatgggat gcgtatcggg
      301 tcgacactga gctatttaaa ccaggcgcaa agccagcaag gaaccccagt aatggttctg
      361 ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat
      421 ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgcctggcag ctacagcgtg
      481 gcattaaaag tctacgaaaa taaccaactc acccgcaccg agcttgtgcc gtttaccaaa
      541 accggcggtc tgactgacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca
      601 caggtttctg atgatgaaag ttcagcttat cagctaggag tacgcctgcc attacatccg
      661 caatatgagc tctacgcagg gctggcgaat gccgatgatg tgagtgcttt cgagttaggt
      721 aataactgga cggcagattt aggcggggtg gggaatcttg caatcagcgc cagcgtgttc
      781 cgtaacgatg acggcggcaa aggtgatatg caacaggcca actggagtaa tccgggatgg
      841 ccgacgttgg gcttttatcg gaccaactct gacggcgatg cttgtacaac cgacagcaga
      901 gagagctata acgccttaag ctgttatgaa agtatttccg cgacggtttc acagaatttt
      961 gtcggctgga atatgatgct gggttatacc cgcacacaaa ataacactga tgatagtttg
     1021 cgttgggata aacagcagag ctttgaaaat aactatcttc gccagacaac agcgcaaagt
     1081 atctctgaaa ctgtacaact tagcgcttcc cgcgcttttg tgatgcgtga ctggattttg
     1141 agtacgtcag tcggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc
     1201 ttgtacttat cgttttcgtt atctgacacg ccaacgatgg atagcaataa caacagccat
     1261 tcaacaaatg tgtctacgga ttatcgttat agcgaacagg atggcgatca aacgtcatgg
     1321 cagttatcgc atacctttta taacgattca ttcagccata aagaacttgg cgtaaccgtc
     1381 ggaggcctga acaccgatac cataaacagc gcggttaacg ggcgttggga tgggcaatac
     1441 ggaaatgtct acgctaccgt atctgatagt tatgaccgta agaatcatga tcatctttcg
     1501 gcctttacgg gcacttatag ctctacgctg gctgtcagtc gctatggcgt taatttggga
     1561 gccagtggta cagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa
     1621 caggatgaag agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg
     1681 cagcttggtc aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt
     1741 gaggttaacg acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca
     1801 gggaatcgtg aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc
     1861 tttaactaca actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt
     1921 gtggggctga acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt
     1981 aacggcagcg aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg
     2041 agtgttgtaa agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg
     2101 acttattcac aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta
     2161 cgtggaaacg ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcag
     2221 cgatattggg cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg
     2281 aaattacgta tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt
     2341 cggcaggaaa taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct
     2401 gtacagctaa tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga
     2461 tgcgttggcc ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc
     2521 cgccaggatg caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt
     2581 catgtacaga agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc
     2641 ttgagcagac agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat
     2701 tacattcgaa cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc
     2761 ccaaccatat tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag
     2821 acttgcatcc aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg
     2881 acatgtgcct gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag
     2941 atgaagggcg accagctgca gggcgcagag acggttactt ctctatctat cgtcagggag
     3001 ggaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa
     3061 ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga
     3121 aacgtgtgcg tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac
     3181 ccttgactct ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg
     3241 gcaaattgtc agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg
     3301 acaaacgctt ttttctatat aaacgacgaa gccgtctgat aaggcggctt cgtcattaca
     3361 acttaagtta aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca
     3421 atcgccggtt tgttctgcaa cacggtccag ataaccagcg cacccagcag gtcaaatact
     3481 gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     3541 gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact
     3601 tcgttacgac cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg
     3661 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc
     3721 atacccgggc caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc
     3781 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     3841 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     3901 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgcc
     3961 aggaaacgcg gcagcgcgat accccagaac tgacggttac gcaggatctg accaacggac
     4021 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     4081 tcttcatcgg tcagatgctt ctggtcgcgt ggatgtttat agaaaatcag ccatgccatc
     4141 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcattacg
     4201 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     4261 taacctaccg cgatggaacg ctctttcgcc ggaaaccatt cggagctggc tttcagaccc
     4321 gccgggatca tcgctgcttc cgcggcaccg actgcaccac gagcaatcgc caggccaccc
     4381 cagctaccag ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     4441 taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta
     4501 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     4561 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     4621 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     4681 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     4741 ttagttacga ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4801 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4861 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaact
     4921 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     4981 cgaggccagt actggcgcgg cctgcagaga gatttaccac tttgagagta atttttttaa
     5041 ctacgtttat tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac
     5101 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5161 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5221 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5281 ggaagactat cgttttaaaa acctgtatga catctggctg aaaggcgatc actacaaatg
     5341 gcgcgctatg cgtaccaacg gtgtggccga gcgtctgtgt accggtgatg cgtctgaccg
     5401 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5461 ctggacgcac ctcgaactgc gtcgtccgtt tggtatcact ggcaaattgc tttctccgtc
     5521 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5581 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5641 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5701 gagctggcgt ccggacaaag ccttcaacat cgaacaggcg acctttaacg actacatggc
     5761 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5821 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5881 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     5941 ggctggcgaa cccctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6001 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6061 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6121 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6181 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6241 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6301 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6361 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6421 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6481 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6541 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6601 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6661 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6721 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6781 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6841 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcgt
     6901 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaatga gctgtggatc
     6961 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7021 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc
     7081 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7141 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7201 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7261 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7321 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7381 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7441 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7501 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7561 gacttcaaac agtactttat tgcccatgac cagccgatct atgaaaaccc atcgccgggg
     7621 aacaaagcgg gcggtatcac cacgctggaa gataaatccc ttggctgtac ccagaaagcg
     7681 ggttccagcg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccggggctg
     7741 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7801 tgccatatgg tgctgttcag tactggtcgt ggcacgccat acggcggctt tgtaccgacg
     7861 gtgaaaatcg ctaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7921 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     7981 atcgttgagt ttgccaacgg taaacaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8041 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8101 gtttcataat gtgaagcgcc gtttgctttt ccatacctga taaggattta tcatgaccgc
     8161 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8221 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8281 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8341 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8401 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8461 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8521 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacaat
     8581 gattgagggt agttttctgc ttatgaatgg cttgaatatc attcgtttct ggcggatgca
     8641 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccgtag acgaaagggg
     8701 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8761 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8821 acgccgatga taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8881 atattacagg ccagcgggat cagcagcaga gaaccccccg ccacgccgga tgcgccacag
     8941 gcacacagag aagccaccac gctcaacagc agcgccgtgg gcagatcgac cgggataccc
     9001 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9061 atggtggcac ccagcggaat agaaacggaa taggtatcgc gatccagatt cagcttttca
     9121 cacagtgcca tattcaccgg aatgttcgcc gcagagctgc gggtgaagaa ggcatacacg
     9181 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9241 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9301 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccaga
     9361 ccaaaaatac caatcggtgc gaagcgaatg accagtttca ccataaaggt gacggcattc
     9421 gacatatcgt tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaaaccgagg
     9481 ccaattgccc acaccaggat cccgatgtag ttacctttca gcagcgcgtc gatggggttg
     9541 gaaaccatgc tcattaccag cccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9601 tcacccgcgc tactggataa atgcagggta gaagggaagg caaagctgaa gactactgcg
     9661 gccagagcag cagagaaggt acccagcaga tagaggaaca aaatagggcg gatattggtt
     9721 ttctgcccgt gctggtggtt agcaatagat gccatcacca gcatcaacac caggatgggg
     9781 gcaacggctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9841 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9901 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
     9961 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10021 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10081 tggattttaa agagaggtca tattttgcaa cttaattgtt acatttgttt gacataaaca
    10141 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10201 tgagaacggt tgcgctcctt taatgtgtta tgccatataa agtcactggc gcagggagcg
    10261 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10321 ccgctgcgcg gttccctcag cttatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10381 ccctgtaaga tgcgccctcg ccgcgcatcc atgcgcgtcg ccccggcctc cagggaacgc
    10441 ttcggcgatt ttgacgccac caacacccgt gcggtctttt tgtaaagagc tataagccga
    10501 ttacataatt aatagctcac ctttgtctgg aatgagttat ctctagtaat ggcttaatat
    10561 acagcggatc attctccccg ctgcttatca tgccgataat tcacccaggc gttgataata
    10621 aacgtcatca ccagaatgcc aaacaccacg cccagcgaga cggcgattgg aatatggtag
    10681 aagtcgacaa tcagcatctt gataccgata aacaccagaa tcaccgccag gccatatttg
    10741 agcatcgaga aacgctctgc tacgcccgcc agcaggaaat acatcgcacg caggccgagg
    10801 attgcaaaca ggtttgaggt cagcacaata aacggatcag tggtcacggc gaagatagcc
    10861 ggaatgctat ccacggcaaa aatcacgtcg ctcaattcca ccagaatcag taccagcatc
    10921 agcggtgtgg cgtacagcaa tccattctta cgcacaaaga aatgctcgtt gtcgatggtg
    10981 tcggtcatgc gcaaatgacc gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat
    11041 tcatcttcat gggcgagcgc catcttcacg ccggtaaaca gcaggaaggc accgaagata
    11101 tacaggatcc agtcgaactg tgaaatcagc cagctgcctg tgaagatcat gatggtacgc
    11161 agaacaatcg ccccgagcac gccatacacc agcacgcggc gttgtaatgc cgccggaaca
    11221 gagaaatagc tgaacaacat cagccagaca aagacgttat cgaccgccag cgatttctca
    11281 atcagatagc ccgtgagaaa ggccagtgcc tgtggatcgg caacggcgcg accttcagtt
    11341 tgcaccagat accaccagaa agcggcgtta aacagtaacg acagcgtcac ccagaccagc
    11401 gaccaggccg cagcctgttt catggtcatg gcatgtgccc cacgacgccc ctgcaacaac
    11461 aggtcgatag ccagcataat ggcgacaaca acagcgaatc cgccccatag caacggcgtg
    11521 ccgacagtat tcatagaagt tccttacaca taaaaaaacg gccaacgtcg gaagacgtca
    11581 gccgctgctt tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag
    11641 gaatacgtct gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc
    11701 gtaatgacga tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga
    11761 ccattcagcc agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat
    11821 agttttacgc tatctgccgg aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt
    11881 ttggcggtga tgcgactgac cgccagcccc ggatgatcca ccagatgctc atccaccagg
    11941 gtagcgaaca gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc
    12001 tgcatttggc tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc
    12061 accagcgatc ctgcttcgcc ctgaatctcg ctcgccagga cagaatcact gacttttgag
    12121 tgctgcaagg tgacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcatctaca
    12181 ccgcttgcca gcaaactggc ggttgcctgc acgcttttcg gctcaccaaa taacgccacc
    12241 gccgacgcca ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgacggatta
    12301 aaggtgttgg gattttcacc gtccaggtaa cgttgatagc gcgaggaata ctggcaatag
    12361 ttgaaaaaga ctttacgcaa tttgccgact ttcggcagcg cctggcgcag caaatgaaag
    12421 ttcggcaggc aggcggtttt aaatgcctca aacagcacca cctgattttc ccgcgcacag
    12481 gcaatggcgg catccacttc cgccagattc gacgccagtg gtttctcgca aatcacatga
    12541 attttatggc taaggaaaag ttgtgtctgg gaaaagtgca gggcattcgg gctggcaata
    12601 tacaccgcgt caatggcatc gctttccgcc atcgcttcca gcgaggtaaa cagatgctcg
    12661 acagaaaaat cattggcgaa gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt
    12721 aacttgtatt taccgctctc atgggcggcc tcgacgaact ggcgagtgat ccagttcgta
    12781 ccaatcacag cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa
    12841 tgtagcacgc ctttttcatc ttcgacgtgc gcagatacgc attattcttt tgccggacag
    12901 ccatgctcac taaacggccc aatcatgacc gatggcccga gatatttcgg ctggcgatgg
    12961 aggattttgg cgtcaatcac ggcgctgaca cgagcgagtt tctcccgcag ttgagtataa
    13021 aaaccgtgct tcatattgag gtcaggcgcg ttaaaaccga tagcgtcgat gccgtactgt
    13081 tttgccagcc agatggcgcg ttgattatga aattcttgcg agataatggt gatatggttt
    13141 tcaccaaaaa cctttttggc acgcactacc gaatccagcg ttgagaatcc ggcatagtcg
    13201 cagaagatca ctttcgccgg tacgccttta gcgatcaaag cctgctgcat acctgaggct
    13261 tcatcgtaat ttttacgtcc gttatcgcca cttaccagta gccatttcac ttttccggcg
    13321 tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctggcc
    13381 cccagcaata agcccacgtt acgcgccgga actgcgttga catcgctcca ggtcagctgc
    13441 ttgctggcgt tgaccataac gcgatcggca ataaaaatgg tcgctcctgc tataagcaac
    13501 aacaaacagc caatcttaag agtccggcga gaaaaacaaa tacggagaag aagacggggc
    13561 tgcatggtcg ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaagaaa
    13621 agataacgcc tgacatcatt ttcagatttt gtcctgccca agcgacaact gcgttagcca
    13681 cagccgggtg tcgaactcaa gctggtggta ctgcggctcc atatggcagc acaactggta
    13741 aaacgctttg ttatgctctt tctccttcag gtgcgccagt tcgtgcacca cgatcatacg
    13801 taaaaacggt tccggcgcgt tgcgaaacac agtagcgacg cggatctctg cttttgcttt
    13861 cagcttgccg ccctgcacac gagaaacagc ggtatgtaac ccgagcgcgt tttttagcac
    13921 atggatctta ttgtcataca tcactttatt gatcggcggg gcattacgca gaaactgatt
    13981 tttcagatcc tgagtatatt gccagagggc tttatcggtg gcgtagtcgt gtgttcccgg
    14041 atagcgtttt gccagcacat cgcccagacg ctgttcgtta atcagcgttc gcacctggga
    14101 aagtagctgc tcgggataac cctgaagata agtaagattg ttcataaccg cccacgaaaa
    14161 agagaaaaag ggtatactca cgcacccttt tcaggggcaa cgtcgaaatt ttatcattca
    14221 ggagggccga tgagccactt agacaacggt ttccgttcac tgacactaca acgttttccg
    14281 gcgacggatg acgttaaccc gctacaggcg tgggaagcgg cggatgaata tttgctgcaa
    14341 cagttggacg acacagaaat ccgcggcccg gtgttgatcc tgaatgatgc ctttggtgcg
    14401 ttaagctgcg cgctggcgga acataagccg tacagcattg gcgactcata catcagtgaa
    14461 ctggcgacgc gcgagaattt acgcctcaac gggattgatg agtcgagcgt gaagtttctc
    14521 gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc tgatcaaagt gccgaaaaca
    14581 ctggcattgc tggaacagca actgcgtgcg ctgcgcaaag tggtcacgtc ggatacacgt
    14641 attattgccg gtgccaaagc ccgtgacatt cacacttcca cgctggagct gttcgagaaa
    14701 gtgctcggcc cgaccaccac tacgctggca tggaaaaaag cgcgcctgat taattgcact
    14761 ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta gctggaagct ggaaggtact
    14821 gactggacta tccacaacca tgcgaatgtc ttctcccgca ccgggctgga tattggcgca
    14881 cgcttcttta tgcaacatct gccagagaat ctcgaaggtg agattgtcga tctcggctgt
    14941 ggtaacggcg ttattggcct gacgctgctt gataaaaacc cgcaggcgaa agtggtgttt
    15001 gtcgatgaat cgccgatggc ggtcgcttcc agccgaatga atgttgaaac caacatgcca
    15061 gaggcgttgg atcgctgcga gtttatgatc aacaacgcgc tctccggagt ggagcctttc
    15121 cgctttaatg ctgtgctctg caacccgccg tttcatcagc aacatgcgct gaccgataac
    15181 gtcgcctggg agatgttcca ccacgcccgc cgctgcctga aaatcaacgg cgaactgtat
    15241 atcgttgcca accgtcacct ggattacttc cataaactga agaagatttt cggcaactgc
    15301 accaccatcg ccacgaataa taaatttgtg gtgctgaaaa cagtgaagct ggggcgtcgt
    15361 cggtaagatt gggagttacc tgccggatgc ggcgttaacg ccttatccgg cctacgttca
    15421 gcacgttact cccaggccgg ataagacgcg gcaagcgtcg catccggcaa tgtctgccca
    15481 gttctggaat cgcgcttcca taaaacaaaa aatccctgtc accatcctct acattctctt
    15541 gtttagcgtt gttcaacgtt tatgctacat acgtgaagtt atgacgcata aagaatacga
    15601 tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc cgacatcttg
    15661 caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca gaacgaggaa
    15721 caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc tgaaaacccc
    15781 ttgctggatc tggtgtgtgc aaaaataacc gcgtgggaag aatcagcgcc cgaatttgcg
    15841 gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg tacccttatg
    15901 gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc tatggtgtca
    15961 cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt ggcaacgcga
    16021 ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc gttgcatccg
    16081 gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc aattgcccgt
    16141 cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt tttcccgcta
    16201 tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat caccacattg
    16261 tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc atcgtcaatc
    16321 ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcagggt ggtgcgatgg
    16381 atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg ctggagcatc
    16441 acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc accagcctgt
    16501 tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg gctggtggat
    16561 tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca accgatgatg
    16621 gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact caataccttc
    16681 ggatgatcga tcccgtcgat aggcggaacg cgcggcacga tcccactggc gaggatcgtt
    16741 tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag cgtcacgccc
    16801 gtcacttcga tcatccggtg gtaatagcgc agcgtttcgt aaaactcctc tttgccgggg
    16861 atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gggcatcaaa caatgttacc
    16921 tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc cgcaccgacc
    16981 accgccagat tttttttctg cacggcagga aggattggca ttttggtttc gtggcaggcg
    17041 cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag gcaggcctga
    17101 ttgcagccaa tgcaggtgtt gatctcatcg gctcgccccg attgcgcttt tgacagcagt
    17161 tccgcatcag caagaaacgg tcgtgccatc gataccatat cggcatcgcc gcgcgagaga
    17221 atgtcgtcgg caacctgcgg atcgttaatc cggttggtgg tcaccagcgg cagcgagacg
    17281 tggcctttca gtttgcgcgt gacccaagta aatgcgccgc gcggcacggg cgtggcaatg
    17341 gtcggaatac gtgcttcatg ccagccaatg ccggtgttga taatggtcgc gcccgccgct
    17401 tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc gaccaggtcg
    17461 agcatcgaca accggtagat aataatgaag tcgttgccga cgcgttcgcg caccgtacgc
    17521 actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg gtcactacgc
    17581 tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcggaccc cattacctct
    17641 acaccgtcgt atcctgcctc ccgtgccagt tgcgcgcagt gggcgaaatc gtcgatcagt
    17701 tgcaggatct cttcatgcgt gagttcgtga ggaacgaaac ggttgatggg ggcctgcaat
    17761 gcggagggag cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa aatttgcagg
    17821 gctattttgc cgccttcctg atgtaccgct tcggtaatgg tgcgatggtg tgggatctgg
    17881 ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc tggtgcaatg
    17941 ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa cgctgccagc
    18001 cgctcggcac cgtccgggta ttcctccagc ccggtgtgca ttgagcccat caacacgcgg
    18061 ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta gctcataaaa
    18121 ttgtccagta tgtaaaataa ttgttatgtg gtcggatgag ttctaattta gccgtcgccg
    18181 gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa tacgcccggt
    18241 ggtaacgccg gatgcgacgc ttgccgcgtt ttatccggcc tgggggattg cacatgtagg
    18301 gcggataagg cgttcacgcc gcatccgcca gtggcgcggt gcagttgccg gatgcaacgc
    18361 ttgccgcgtt ttatccggcc aggaggattg cacatgtagg gcggataagg cgtttacgcc
    18421 gcatccgcca gcagttgcaa attattgctt acggaaaaaa tcgttataca acatatacaa
    18481 atgcgcggca ctccaggaga aatttggtgc gccttgctgt gcgcctgtca gcgggttgta
    18541 attttcctga attggtccat cggcggttaa ccctttggcg tgccggaaga acgtatccgc
    18601 cagcttcagg gcatcatcac gataaccgta acgctccatc cctttcagac caaaccagaa
    18661 ctgatccacc catacgcgcc cacgccagta gatatcagcg ccaaaagccg gattggttaa
    18721 cgccgccgtt cccagcggga caaaggtgtt gaactcttta ggatcgagca tcaccttcac
    18781 caccgcgtcg gcattggcct gcgttgccgc accgttaaac agcggcgacc agccttccgg
    18841 ccctttaccg cgctcaacaa tcggtttgcc cgcgcagccg ttcgccagtg gtttatcttc
    18901 aatacgtacg tcatagtaga actgggtagt cgggtcgaac atacaggtgt tgatgtagtc
    18961 cgcgagctgc tgtgccaact ggcgatagcg tttggcctct tctggcttac cgaggatggt
    19021 tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat ccaccgactc
    19081 ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg cgaatttcac
    19141 cgtccagtcg ctacgtttgc cgccgcttgc aacatattta tccagctgtt ctttgtcgat
    19201 aaaaccaaag acggcggcat catcacgccc cgattcccac gacgcagcga cctgtgccgg
    19261 aatttccaga ctgtcgtact ggcctttctc caccacgcgg gcgtagttgt tcagcccaga
    19321 ctgcgtctct tctttgttgc ctttcttcac cgtaaacagc atctcgccgc tctcagtgtt
    19381 gtgggctttg tcgcgggtcg cgccatattc cggcacgccg ttgccgttgt gatcgcggtt
    19441 acgtaaccac cagtcgtgat aggccaccag tttcgggtac atctctgcca accaggcttt
    19501 atcctgggtg acgttgtaca cttccatcac cgaccaggcg gcaaggctgg gtttggtatt
    19561 acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc acgctatcag
    19621 gtcggggaca aagcccacat cctgcggacg cacgctatcg ccaggctgga tctgccagga
    19681 gaagaccgcg cggatattct ctttggcgat gtccggattg aaatgcgcca tcgcaaacgc
    19741 ctgcttccag gtatcccacg gccaggtctg attgccggag aaccagcgcc cggtcaccga
    19801 cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag cgccagttac cgttgagcgt
    19861 ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat tggttaaccc
    19921 tttcttcagg tattcttccc agcgttgctg cgaggcggtg agataaaacg ccggacgcgc
    19981 cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagcggtca gcagatggga
    20041 ataggtggtg tagagcgtgg tcgaaccgtt gatatgcgcc ttactggtaa agcgattacc
    20101 gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt cgccggaggt
    20161 cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat cacgggtggc
    20221 gctgattttg cgctgatagt cagggtattc gccagcaatg gttttatcgg aaagcggttt
    20281 cccttctttc gcttccagtt tttccagcag ttcgccgtcc cacaccagat ccagcggttt
    20341 attgctggtg attttggttt ccagtagtga cgtgcgcggc gtggcgaagc gcagagtcat
    20401 ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc gcaccgggaa tactgtatgc
    20461 ctccagcgta aagtcgactt tcttgccgtc ctgccagacg gtcaggcggt cgaaattgct
    20521 ggccataaag ttgatgtact cttccgtcag cagcgcaacg cccggaaagc cgcccatggt
    20581 gttagggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa acggattaaa
    20641 acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcgccag tacggttaat
    20701 cacgttttta taattgtcgg cgttagcggc atgggcggaa aaacttatca gcagagcgca
    20761 ggttactggc gttaaaatag tttttatttt catgaaatta cagtccttat taccagtaga
    20821 aatattgcat caggaagatt tggtcgctgg gatcgtgaga ggcatttttc atgtagaagc
    20881 ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc gtaccagata ccaaactgca
    20941 tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag gtgtaagcgg
    21001 tggagagata aacgcccttt aacgcttcat acatggcgct caccatcatc ccactttcac
    21061 ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg ccaggcgcga gcggcaaagt
    21121 taggagttag caggcccgtg agatagagct gtcctgtgcc gtcggtgatt tccagaccgt
    21181 tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc attgccggat
    21241 aggtgtattt gtcgtcatag cgatcgtact tgccaaaatg tagccaggct ttgctttcgt
    21301 catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa gttgccgctt aagttctgca
    21361 ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac gtagcccaga tcgggggtga
    21421 agtcgcccca ccattgcagg tcatccagcg aggaatcctg acgcagactg agcatgattt
    21481 cggtgccatc attggtacga taaccgccat agaaacggtt aattccgccc tcgaaaccac
    21541 cccagctatg gtctggcacc caggcattgc cgtcgtggtc ggcctgcacg gtccagcctt
    21601 cgccgtaaat caggccgaac caggagccgt acttcatctc caggccacct tcaatatagg
    21661 taccgtcgct gtatttatgt ttattctcac cctctaaatc catatatcca ccaaccccta
    21721 attcgccgta aaaacgaaat gaaggagaag atgtcgaggt aattgcaggt gtttttgtgt
    21781 cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag caaaacggca
    21841 gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat tgtgttaatc catgagctaa
    21901 agaaaaagta cagcctttta tcgttgtaat tataaaaata taaaggcaga aactattttc
    21961 cctctcctca tccaggaaga gggaaaatgc ctgctcattt tttattattc tgcttaatca
    22021 acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag ccgttaatcc ctttatatat
    22081 ttactgtatt tccaccacgc aaagccaagg aagataacaa taccgccgac gttataaaaa
    22141 atgatggtca gaatattcgc gccagtcggg aatgtcgaag cgacaaaccc tacggcaaat
    22201 atcgcaatca gcattgaaac aacgataata ccggtgcggc gggagcccat acggaaatcg
    22261 cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag ccagcatgat gaataacggc
    22321 ggaagcatgg acgctgcggc ggtcatatta ataatagtat tcatcagatc ctgcacggta
    22381 ttggaaccga gcatcgggat aatcatcagc gggatgacga tcaggaactg gatccacgct
    22441 gcgcgcgccg gaacgccgtt ttcgttcagt tcgacggttt tcttaccaaa gatgccttcc
    22501 gggatttcgg agaagaaaat tttcaccggc gttgcggtcc acatcagcag ggaaccgaac
    22561 atcgcggtaa aggacaccag accgacaaag cgattcatca acgcttccgg tagaccaaaa
    22621 tacgccgcca tgccgtggaa tacctgcacc gatccgccgg taaatttcaa ctctttgctg
    22681 ctgacgaaga cgttaatcag caccgaggag acggaataca gtacgccgat aaaaatcccg
    22741 gcgaggatga tcactttaac gaacgatttc gaaccgcctt tgacgtcgtt aacgtacaca
    22801 gcgacggact ccgcgccacc tgcggccata aagatccagg tggtaacgcc gaggaacgcc
    22861 cagttgaagt tcgggatcat cgcgtcaacg gtgatggggt cagcaggctg tacgccgcca
    22921 accagcgccg tacctgccag taaaatgtag gagagcgtta acagcagcat cagcgttgaa
    22981 gtgacggagg taattggccc cagcattttc gccccgttgg tggaaaccca ggtggagaag
    23041 gcgaacagca ccatactgat aatggtggtg gcaaccggcg tcataatata ttcgtagccg
    23101 aggaaggcat acgaagcata agcaataacg cgcggcaaca gtgaggtgaa aaagaacagg
    23161 tttacgaacc agtaggtata ggcagtaata aatgcccaac gtccgcccag cgaacttttt
    23221 acccacgcgt agacaccggc ttctgagttt ttatttaacg aaacaaattc tgcgatgatc
    23281 agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa acatcggtgc cgaggccagt
    23341 ccaagctcaa tattattatt gataacgttg ttaaagctgt aaacggcggc aaaagtcagc
    23401 gagagcaagc cgaatttgcc gattgtatta cgtttggtat cagacataac acatctccga
    23461 aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg ccgtagttgt tatttgttat
    23521 ggaaataacc atcttcgatg gtgactttga gaaccacttt tttgaccgcg ttattgcaga
    23581 taaaccgata cgcttcatgg atatcgcaaa taacgatttg cccttcgtgg acctcaacgg
    23641 tttctccgca gccttttaaa tattcacggt cagtttcatc acgataatat tccactacct
    23701 gtaatgtctc tttcggcgca tattcaattt tttgctgccc ttgcaggtag taatgcactt
    23761 caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc ggagtctgtc tccacgcggt
    23821 aagtcaatga gtcaccgatg gagtgggcga cgccaggctg aatgttgtcg atattttcaa
    23881 tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta aatctggcgg aactgttcta
    23941 agttatcgat gatcctcatt gcttattctc gctgtgcaaa ttcgtggaaa agaaccctgc
    24001 gccgaactca tacgacgcca ggctttgcgc ggtagcttct ccgccagaaa ccggcagcaa
    24061 cgtaaagccg tagctgaagt cacggaacca gacgcgccag gagtccagca cctcgctgcc
    24121 ccaggagttg gagccgaggc caagcagctg gtggtcgagg ttcagggtga tgtcatcact
    24181 gcgctgcagc tcgttacagt gctgggcagc gtggatgttt tcctgggtat agcgccaggc
    24241 gctgaagtta attgggcgct gcggaaccac cagcagaccg ttgccgtggc ggttagtcag
    24301 tgccgtccag cggacatgct gacggttgcc gttgttctgc gggaagggat agttctcgaa
    24361 catggcatcg acggtgctgc gccagatatc gatgatgtta gcctgctggc tgtcggcgta
    24421 gttttcgccc ggtccacgac cgtaatacgc cacctgatcg tattcgccgt taatccccat
    24481 ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg taacgctcgc cggaaagcgc
    24541 cacgttaacc tggccatcgg cagcgatgcg ccagatgtag gtgcagcgca tcccgaagtc
    24601 aaacaccggc ggggcaataa ctgtgcggct gatgatcaac acttcaccat cgctctgttc
    24661 tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa tgattcggtt gccacagccc
    24721 ttcgtactcc tgcttgtggt tgtcgatcat tggcttgaag aagttgatct ttggctcgcg
    24781 ggtcagcagc gactcgccat tcacctgcca ggatgtcggt ttgccactca tttttgagaa
    24841 ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa cgatcgtctt ccagcgtcag
    24901 cggacgcgca ttatttggtg cgaaaggcac tggttgcgcg gtgttttcct tcagcgggaa
    24961 ctgataagtg gcgatagaat gcccggcttc gctgtagcgg gtgcgggaat ctttggtcac
    25021 cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc agcgtgatct gcaagggggc
    25081 ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc gtcgcgagcg tttcaccttc
    25141 gacgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg gtaaaccaca gtttattttc
    25201 gactttcagc tcgccgcgag tcagatccag cgcgtggatt tttaccggcg cgataacctg
    25261 tttgtactct ttcaggcccg gtcccggcgt ctgatcggaa tagatcaaac catcaagaca
    25321 gaagttatag ttgttgggat agtcgccgta gtcgccgccg aatttatacc agacgttgcc
    25381 gttgtcatcc tgcgcctgga tcccgtggtc gcaccactcc caaacataat gtccctgaat
    25441 acaatcgtgc ttatagaaga cgttctggta ctccgtcagc ccgcctggtc cgttccccat
    25501 cgcatgagca tattcacaga tgatgcgcgg cttcggatgc gggtattcac caaactcatt
    25561 catcagcggc acgcgggtgt acatggtgga aataatatcg accacttcag catcgcgatc
    25621 ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgccttc gctgcgtggt acatcgcgcg
    25681 gatgttacag ccatagccgg attcattgcc cagcgaccag atgatgatcg acggatggtt
    25741 tttctgcgcg tgaatatggc gaacaatgcg ctcgacgtag actttttccc actgcggatc
    25801 gtcggtaata cggctgatat cgccgacatt agcaaagccg tgcgattcga cgtcggtttc
    25861 cgccatcaca aacaagccgt agatatcaca cagttcgtaa aaacgcggat cgttcgggta
    25921 gtgagcggta cgcacggagt tgatattgtg ctgcttcatc aactggagat ctttctcgac
    25981 gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg
    26041 cagcatcaca taacggttat tgatccagaa cagaccgtcg cgcactttga tatcacggaa
    26101 gccaacgcgt tgtggcacca cttccagaac gttgccgttg gcgtctttca gcgtcatgac
    26161 cagatggtaa agataagggg attctgctga ccattgctgc ggctgttcga cagtaaaagc
    26221 aaagctggcg ctggtcagtt tttcaattgc caaatgatca atggcgctgc tgtgcaccac
    26281 gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg acaggggagg cggcgagatt
    26341 ttccagcacc acttcgcagg aaagtgtggc atcgcaatag gcttcgtcaa agtcggtacg
    26401 cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc agataaacat cgcggaagat
    26461 ccccgctgac caccacatat cctggtcttc cacgtaggta gagtccgccc actgcatcac
    26521 gcgcacacac aacaggttgt cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt
    26581 caggcgactg cccttgctga aacccacata ctgaccgtta acatagactt caaaataggt
    26641 ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa
    26701 aatacgttga taggcaccgg ttgggttatc gctggggaca aacggcacat cgatggggaa
    26761 cggaaaacct tcgtcggtat attgcagttt gccgtgacct tccatttgcc acatggcggg
    26821 gacggtaata tgcccccagt cagccattaa ctcagaggtg aaggcttctg gtacttgcag
    26881 cggatggtca aaaaagtgga aattccactg accgcttaag ggcagaaaca ggctgctggt
    26941 ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg
    27001 cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag cgattcatag ggcctcccgg
    27061 tagagagaaa tcggaaccat tcatggtgat ggtagtttgt gtaattgctg gggagtttag
    27121 taaaattttt actaaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata
    27181 aacggcagca ctgtccggca aaaacgattg ccggacagtg tgtaagggga tttaacgggt
    27241 cgtgccgcgc agttttaatt tgctgggaac gaagactaac agcggcagcg cgcgaccatc
    27301 gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg
    27361 cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag
    27421 cgaaatatcc tgtgggatgt tcaggcctcg ttcatgaatt gcccgcagta cgccgatagc
    27481 aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt
    27541 tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac
    27601 cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg
    27661 ctcatcttca ccgcaaataa aaccaatacg attaacgccc tggttgatat agaagtcgat
    27721 gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc
    27781 gtgaaagtcg ataaaacaga tattgtcggt caacgcacag gcggcggcgc gcagggcggg
    27841 cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta atccgctgtg
    27901 ttcataacag ttggtgagct caatgcccag cttttcgcac tgagtttcaa tgccgtggcg
    27961 gatcgccaga tagtaaggat cgttgatctc cagctcctgt tggtagctgt agatagccag
    28021 aatatggtgt tggttgactg cacctgtctg gagtttacgg gcactactgg tcttgtactc
    28081 cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg
    28141 atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga ttgcgatgtc
    28201 ttttagtgtt gccatgttta ccttttttac tttccctgat gtgcgccccc attgtagagg
    28261 aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga cgctgtaacg
    28321 gtaacacagc ggctcacgaa tgtgatcacg cctgcattct ccatttttag cctacaggat
    28381 tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa acaatggaaa
    28441 ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga
    28501 aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg gaaaaaacac
    28561 tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga agagctgatg gggaaaacgg
    28621 tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg tgaaacgtcg gaatgcatgc
    28681 tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt gttaacgccg gagcggatga
    28741 tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc gacgtcgtat cgccatccga
    28801 tttgatatta cgcttcttcg acacttactc gcatggccgc cagcgcctta cgcgccgctt
    28861 tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg cggttcaatg cggatcgttt
    28921 tggcgttatt gagcgttccg gccaccagta cgcgctggcg gaacatctcg ctggcaaagt
    28981 tatagccgat ttcgttatca acaaactcaa ttgccatcaa catcccttta ccacgcgctt
    29041 cctgtaccag atcgggatat tcccgcgcca gttgacggaa accgtccagc aacatatcgc
    29101 ctttttgctc agcctgagcc ggtaagttct gctccagcaa cacattgatg gtcgccagcg
    29161 ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt atgcaggaat gggttgtcga
    29221 acagaactga aaacacctct tcagtggcga tggtcgcgcc aatcggcatc acgccgccgc
    29281 cgagcgcttt ggcaaggcag aggatatccg gctgtacgtt ctcatgctcg caggcgaaca
    29341 tcttgcccgt gcgccccatg cccgtttgta cttcatcgag gatcatcagt gcgccgaact
    29401 catcgcatag cttacgtacg gcggtgagat agcccggcgg cggcagaatt acgccacctt
    29461 caccctgaat cggttcgagg atcaccgcag ccacatcatc accggttttt ttgcactcgt
    29521 taagagccgt gcgcatggct tcgatattgc caaacggcac atgacggaag cccggcagca
    29581 gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc cgacagcgcg ccaagtgatt
    29641 taccgtggaa cgcgccgctg gtggcaataa aagtaaactt gccgcgcggt gactggtaag
    29701 ctttcgccag cttcagcgct gcttcaacgg actcggtgcc gctattacag aagaagctgt
    29761 atttcagttt accgggcgtt agcgcagcaa gggttttcgc caacatcgcc cgtaacggat
    29821 cgagcagctc ctggctgtgc agcggttgtt tcgcaagttg attctgtacg gcggaaacca
    29881 caactggatt acggtgcccc acgttgaaaa ttccaaaacc tcccaggcag tcgataaact
    29941 cctgtccctg ggtgtcgaca agcgtattta aacctcccgc ttgccactct acggctccgt
    30001 aatccccgcc ggcggtaaca gatttgcgat actctaaaaa ccccggattg acatgctctt
    30061 tgaagtattc aatcacctct cggttaagtg ctttcatctc ctcatgatcc agcgttcgct
    30121 tctcaatgag attcagggcg tgggcgctgc acgctaaagc cgatgcgctc gaaggtaacc
    30181 tgttcaaaat atgctccgga ggctcgcgta tcacatgata ccgatttaag tattgcaggg
    30241 attgcgccac tccggctgcg atcgcgaaaa tcgggataaa cacaaattcg gttatcattt
    30301 gtgcaatatt taatgctatc tgttaacatt tgttgttatt ttgcacggtt tttgtcgggt
    30361 atggatttga tgattgctgc gcaaatgcac gttttcaggg caataattgc ctgatttttg
    30421 ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa gtattttaaa tcaaagaatt
    30481 agatttgtgc tttaatcgca aattgcgatc taaatcaaat taatcggtta aagataaccg
    30541 cagcggggcc gacataaact ctgacaagaa gttaacaacc atataacctg cacaggacgc
    30601 gaacatgtct tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg acgataccac
    30661 tctgatgtcc actaccgatc tgcaaagcta tatcactcat gctaatgaca cttttgtgca
    30721 ggtgagcggc tttaccttgc aagagttaca agggcagccg cacaacatgg tgcgtcaccc
    30781 ggatatgcca aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag gggagccctg
    30841 gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat tattgggtgc gggccaatgc
    30901 ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta cccgggcgac
    30961 ggatgaagag attgcggcgg tggagccgct gtacaaagcg ctgaacgccg gacgtaccag
    31021 taagcgtatt cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt
    31081 accgcttcgc tggcgggcgc gtggagtgat gaccctgatg tttatcttgc tggcggccat
    31141 gctttggttt gttgctgccc cggtggtgac gtatttcctc tgtgtgttag tggtattgtt
    31201 ggcaagcgct tgttttgaat ggcagattgt gcgcccgata gaaaatgtcg cccgtcaggc
    31261 actgaaggtg gcgaccggag agcgtaatag tgttgagcac ctgaatcgca gcgatgagct
    31321 ggggctgaca ttacgcgcgg tagggcagct tggcctgatg tgccgttggc taattaacga
    31381 tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag acgctggcga aaggcaccga
    31441 tgaactgaac gaacataccc agcagacagt tgataacgtt cagcaaacgg tggcgaccat
    31501 gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg gcgtcggctg ccgataaact
    31561 ttctatcacc gccagtaatg cggcagtgca gggtggggag gcgatgacca cggtgatcaa
    31621 gacaatggac gatatcgccg acagtaccca gcgcattggc accattactt cgctgattaa
    31681 cgatattgcg tttcagacca atattctggc cctgaatgcg gcggtggaag cggcgcgtgc
    31741 cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg
    31801 cagcgctaat gctgccaacg atattcgcaa gctgattgat gccagtgctg ataaggtgca
    31861 atccggttcg cagcaggtac acgccgccgg acggacgatg gaagatattg tggcacaggt
    31921 gaaaaacgtc acccagttga tcgcccagat tagccattca acgctggaac aggccgatgg
    31981 gctttccagc ctgacccgtg cagtggatga gcttaacctg atcacccaga aaaatgccga
    32041 gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca gccgactgga
    32101 agacgcggtg acggtgctgc attaatcggt atgccggatc cggcggttgg agcacaatgc
    32161 ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt gtaggccgga
    32221 taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgatt
    32281 aatccagttg cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc ttttttattt
    32341 gtgcatcact gatatcgctc tggttgacgc gtaaatccag caccgcttta aaattatcca
    32401 gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc gctttgatgc
    32461 gttcttcaat catctccacc tgttcgcggt tttcttccag ccactgcgcg ccttgttcgg
    32521 tcagcgcaat ctgcttctta ccaccttcct cttcgcggat ggtaatcagc gactgctcct
    32581 gcagaaaatc cagcgttggg tagatgacgc ccgggcttgg ggtgtaattc ccctgggtta
    32641 ggttctcaat cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag agaatatcca
    32701 gaatcaccag acgcaattca ccgtgaccaa agaaacgttg ccgacgaccg ccgccgcgac
    32761 cgtggcgacc accgcagcat tgaccgtgtt catgctggtg accgtgtccg cagtgctcgt
    32821 gttctgactt ctcacctttg cagcagccct catggcgtgg ctggccttca tgtttacaac
    32881 acccttcgtg atgatggctc atatcagcct ccttttgctt gattagatat atctaattta
    32941 tatctgatgt gagcactctt gcaagaataa aaatgatttt taaatgtgga ctcatctcac
    33001 acgtaggccg gataaggcgt tcacgccgca tccggcgttg agtaatgatg cctgagaaga
    33061 ggcggttcgt cttgtcagac tatagacata tcagttctac aaatcgcttg catttatcat
    33121 gattaacaat cattatcatt tgcgaaaatt atttagatat atctgattac catcacgaag
    33181 gcgataacaa tgaataactc cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc
    33241 cgtgaattga ctgtgctacg cgttgagcgt attagcgccg gttttcagcg cattgtcctt
    33301 ggcggcgagg cgctggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc
    33361 tttcctcaat ctgatgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg
    33421 ccggaaggac cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga actacgccat
    33481 gaactggcga ttgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgatgcag
    33541 gcgcaaccgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa
    33601 gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc
    33661 ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg
    33721 gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca
    33781 catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac
    33841 ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg
    33901 gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata
    33961 tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc
    34021 tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg
    34081 gccatggtag cggattataa agtaactccg tgtcgcaatc cagccattac cgtgcgcctg
    34141 ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg
    34201 agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa
    34261 ccctgggctt tcatccgccg ggactggttt tccctttgct catccagcaa atcgcttctg
    34321 gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca
    34381 tctgctggat tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa
    34441 tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga
    34501 ttatcagccg caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcca
    34561 gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct
    34621 gccaaatccc agcggtttaa gtcgcgtttc actagagaaa ctggttgaag cgtatcgcga
    34681 gctggaccag gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa ggtggcgttt
    34741 gtgcaaaggg actagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt
    34801 ccaggaagct acgcaacact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg
    34861 cttcgatctg acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg
    34921 tgtggtcggt gttcatatcg ataccgaaac gcatacgcag aactttcgct tcacgcgcgg
    34981 tcaggccagc cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca
    35041 gcggcagctc gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac
    35101 cgatcggcgt ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt
    35161 cttccggcat cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt
    35221 gcagcatctg gcgagaaata cggttgagtt tgttgatagt ctcaatcata tgcaccggaa
    35281 tacggatggt gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg atccaccagg
    35341 ttgcgtaggt ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca
    35401 gaccgatgtt gccttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct
    35461 tggcgataga aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg
    35521 ctttcgcttc accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc
    35581 cggtttcttc ttcaatctgc tgcagtttct gcagggcgcg atgcacttct tcagagacat
    35641 cgtgcagttt ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc
    35701 tggtttcgtt gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa
    35761 cgcagagctt catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc
    35821 tgttgaccag gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca
    35881 gtttcaggat ctcttcctga gcggcagcgt gactgcgacc tttcgctttg atggtgtcac
    35941 gcgttacaac gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga
    36001 tgctgttgtc atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat
    36061 cgtccagatc ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt
    36121 cttctgcgtt cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt
    36181 caacacgatc gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc
    36241 attgaacctg gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg
    36301 tcaacagttc aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc
    36361 cgatttcaga ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg
    36421 cggtgttttc agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc
    36481 ccatgtcgtt gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg
    36541 gcagatggtc attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa
    36601 gaagtttcag ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg
    36661 aggttggtgt tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt
    36721 tgtttaccgc caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg
    36781 cggctgtcgg gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc
    36841 cagctcctgg tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg
    36901 ctcacgagcg attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag
    36961 tgagtcggtg aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag
    37021 tttttcaagg gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt
    37081 cagacctggc tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag
    37141 cttattttca tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac
    37201 cagcaaccct ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac
    37261 gccgctctct gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa
    37321 tttgttgcct aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat
    37381 caatggcagt gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg
    37441 cggcatcagg ctgttaaaca gaaatgcgga aagcggcatc gcctgctcca tccgcgcttc
    37501 aaacgcttct ttaccctctt ttcgtaccag cgtgtcaggg tcttcgccat ccggcaaaaa
    37561 cataaagcgt agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca
    37621 tgcggcatcg cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg
    37681 gaacaacagt tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt
    37741 aatgccgtat tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt
    37801 gggttcagcg ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa
    37861 aatgtctgtt tccggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc
    37921 aaaaccaatc acccgaccgc gtttatcgcg aatggggaac atcacccgct cgcggaaacg
    37981 atcgtaactg cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg
    38041 attttctgga ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc
    38101 aatcgcaaag cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc
    38161 agacgtggca acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa
    38221 ctgataaagc gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa
    38281 tggcacttca agattgtgca ttgctgccag ctcttcgacc gtttcaacga actcgagctt
    38341 gtcgtagttc atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta
    38401 aaactgtttc tcaccgttaa cggtgaatga cggggttttc tcgttgtgga atggacaaca
    38461 cgcgtggaaa ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat
    38521 gtcagtgcgt gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc
    38581 cgatttttag caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt
    38641 acaactacaa ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg
    38701 cggcgtgcgt tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg
    38761 cgcttacgtt cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca
    38821 cctgcttttt cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca
    38881 cgtactttaa ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat
    38941 caacgccagc ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa
    39001 gcaccgcggc ctttttgaaa gggactttta taagggtgag gagtatacac gaggctttct
    39061 ccaggggcga acaaagtttt acatcaaccc gcattggtcc tacactgcgc ggtaataaag
    39121 cgaggtaaaa caagtcatgc gtgtactggg tattgaaact tcctgcgatg aaaccggcat
    39181 cgccatttac gacgatgaaa aaggtttgtt agccaaccaa ttgtatagtc aggtgaaatt
    39241 gcacgctgac tacggcggcg tcgtgcctga actggcctcc cgcgatcacg tgcgcaaaac
    39301 cgtaccgttg atccaggcgg cgctaaagga gtctggctta acggcaaaag acattgatgc
    39361 tgtggcctat accgcaggcc ctggattagt cggcgcactg ctggttggcg caaccgtggg
    39421 gcgttctctg gcgtttgcct ggaacgttcc ggcaatcccg gtacaccata tggaagggca
    39481 tctgttagcg ccgatgctgg aagataaccc gccggaattt ccgtttgtcg cgctgctggt
    39541 gtccggcggt catacgcagt taatcagcgt gactggcatt ggtcagtacg agctgctcgg
    39601 cgagtctatc gatgatgccg ccggtgaagc gtttgataaa accgcgaagc tgctggggct
    39661 ggattatcct ggcggaccgt tactgtcgaa aatggcggct cagggtactg ccgggcgctt
    39721 tgttttcccg cgtccgatga ccgaccgtcc ggggctggat ttcagctttt ctggtctgaa
    39781 aacctttgcg gcgaacacga ttcgtgacaa cggcaccgac gaccagacgc gtgctgatat
    39841 cgcccgcgcc tttgaagatg cggtggtcga tacgttgatg attaagtgta agcgagcgtt
    39901 ggatcagacg ggctttaagc gactggtcat ggcgggcggc gtgagtgcta accgcacgtt
    39961 acgggcgaag ctggcggaaa tgatgaaaaa acgccgcggc gaagtgttct acgcgcgtcc
    40021 ggaattttgt actgataacg gcgcgatgat cgcctatgcc ggaatggtgc ggtttaaagc
    40081 aggcgcgacg gcggatctcg gcgttagcgt gcgtccgcgc tggccactgg cagagttacc
    40141 ggctgcgtaa aactatccgg tgccggagag caatttccgg caccgtcctc acttaaagca
    40201 acaccacggg catccacagc aagccggtaa tcaccagcaa cacgaggaat atcagtccaa
    40261 aaatcgcccc cagtcgccag taatccaccg ttggcagata accactaccg tagtaaatcg
    40321 gactgggtcc ggtggcgtat ggtgtaagaa tgctccccag accaattgcc gcgccaacca
    40381 tcaggcagaa caccggcagc gggatttccg gcatcgccag cgcagcggca atcatcatcg
    40441 gtgcgagggc ggaggtatac gccgtggcgc tggcgaaaaa gtagcgcagc agataaaaca
    40501 ccacaatcaa cgccaccatc accatcgttg gcgaataacc gcttaagctg cctgctaaca
    40561 gtttgccaaa ccagctaata aaaccggtgt tgttgagtcc ggtagcgagg gtgataagcg
    40621 aggccagcca gaagaaaacg ttccacgccg ctttattact gacaatgtcg tcccaactga
    40681 taatgcgcag aagcagcatc agtgccacca cgctgtaacc gaccatcgcg gcatcgatat
    40741 aatcaccgcc gaaaatccac agcaccagcg cgcctaccat cagccccagc atccgttttt
    40801 cacgcgaaca gagcgggccc attgcctgca gttccgtctc tgcccagcgc ggcacctgat
    40861 cgccagactt cagtaccggc gggtacagca cataagccag ccagggaacc agcagcacca
    40921 gcaaaatact gagcggcaac atcccgagga accagtcgcc ccaactcagc gtggcgtgag
    40981 atgcgctttt catcagtcca attaacagca agtttggtgc catcgccgtc aagaaaatgg
    41041 cgctggtcac gcagtcggca acaatcccca tccacatgat gtacgagcca atgctgcgcg
    41101 aactgctgtc gtttggttgt gattgataga gcggtggcag gttacggatg atgggataga
    41161 taatccccgc accacgcgcc gagttggacg gtgttacagg tgcgaggatc agctcggaga
    41221 acatcaccgc atagccgaga aacagcgtgc gatgtcccat ctttttcacc agaaacagcg
    41281 caatgcggcg tccaagcccg gttttttcat agcctgtgcc aaacataaag gcggcgaaaa
    41341 tcagccagat aaccgaatta gaaaaaccgg aaaccgccca cgagagggat tttgcagtga
    41401 atttaaagcc tggctgggcg agctgttcgg ggctgaacag cagccaggga gagagtatgg
    41461 cgatgatgga gatacccacc atcgccacca cggcacccgg cacgggttcg aggatcagtc
    41521 caacgatcac gccagtaaaa acggcaaagt agagccaggt atggttctcc agccctgcgg
    41581 gaaccggaag tagagcaata atggcgatga ccgccagcgg cgcaagatat cgccaccatt
    41641 cagtggaagg tttcatcgtg ttatgcctgc gaaaaaggag aggggtgagc ctctccggga
    41701 gggttatttg atgtaatgga cgtgctcgca gatctcttcc acgatgggat cgcggcgttc
    41761 ggcgaacagc tttttgtttt cggctatcag gttgttgccg tgggtatcaa tagagacaat
    41821 cagcgggccg aactctttga cccggcagac ccacagtgac tccggcatcc cgagttctgt
    41881 ccagtgcact tcttcaatct cttctacctg ggttgccgcc agcaccgcgc agcctgccgg
    41941 gaaaatcaca tgtagcgcct tgaatttctg gcagccttct tcggtcagcg gccccatacc
    42001 acctttgcca accaccagtt tcacgccggt ctgctcgata aattcacgtt caaaactttc
    42061 catacgcata ctggttgtcg ggccaacgga aaccatctcc catttatcgc cgtttttgcg
    42121 cacgatgggg ccagcgtgga aaatcgcttt gccgttgaga tcgtaaggga tcggacgttt
    42181 cagttcgatc aagcggcggt gacaaacgtc acggcaggtc accagcgtac cggtcaggta
    42241 gatcacatcg ccgacgcgaa tatcttgcag atcttcagct ttgatcgggg ttgtcaggat
    42301 ctttttcata acgcgctccg ggtgtgagac agattttcaa aggtgagatc ggcatgaacc
    42361 agcagtgtgc cgcgacgatg cgcccagcag cctgtagaaa cagcaacgcc gatggttgac
    42421 ggatggcggg cggcagattc gatatgcacg cccatcactg aactgttgcc agtcagccct
    42481 tgtgggccaa ttcccagacg gttgagtcct tcttccaggc gtagctccag ctctgccgct
    42541 tttggattgg gatggcggct gccaatcggg cgcaaaatgg cttttcgcga gagcacggcg
    42601 gcggtttcca ccgaggtggc gatgcccacg cccaccagta ccggtggaca ggcgtttacg
    42661 gcgagggtgg agatattttc gaagacgaat ttcactacgc cttcgtagcc ttctgacggc
    42721 attaacactt tcgagcggcc aggtagcgtg cagccgccgc ctgccatgta aacttcgatt
    42781 tccgcatcgt cattgtcggg gatgatgtcc caggtgaccc acggtacgcc gctaccggta
    42841 tttttgccgg tgtttacttc gtcaaaaatt tctaccgcat tgtgacgtag tggcgctttg
    42901 acggttgcct cttccacggc ttgtttgagt atgctttgca gctcgccaag cagtgggaag
    42961 cgggaaccga ctttaacgaa gaacataatc tccccggtgt cctgacaggc aggacgattc
    43021 aggtcaatcg ctttttgcat gttgtcgaac atcgtatggt agataatttt ccccatcgac
    43081 gacgtttcgg catcctttag ctgttttagt ttatccacca cgtcatcagg cattcgggta
    43141 gaaatcatgg cggtaaagtt agcgacaatc tctgtcaact tattcactgc ctgttgctta
    43201 ttactttcgc tcatcatggc aactccagtt tttgtatttg tctggaaaaa ttcaccgcca
    43261 caaactacca ctaaaaatgt ggttttatat tttttgccac cgtgaatatg taattaacag
    43321 ttcgtgaatc aacgcgaaaa taagaatgaa tattgcgcca gagcagggaa tggcaatgtt
    43381 ttgcgaaata cactgcgctt atcacaactg cggataatta acgatgctga atagctggcc
    43441 tttagccaaa gacttgcagg tactggtaga aatcgtccac agcgggagtt ttagcgccgc
    43501 agcggcgacg cttgggcaga cgcctgcatt tgtcaccaag cggatccaaa tccttgaaaa
    43561 tacgctggca acaacgctgc tgaaccgctc cgctcgcggc gtggcgctga ctgaaagtgg
    43621 ccaacgttgt tacgaacatg cgctggaaat cctcacccaa tatcagcgac tggtcgatga
    43681 cgtcacgcaa atcaaaacgc gcccggaggg gatgattcgt attggctgta gttttggttt
    43741 tgggcgcagc catattgcgc cagccattac cgaacttatg cgcaattatc ctgagttaca
    43801 ggtgcatttt gaattgttcg atcggcaaat tgatttagtg caggataata ttgatctgga
    43861 tattcgtatt aatgacgaaa ttcctgatta ttatattgcg catctgttaa cgaaaaataa
    43921 aagaatatta tgcgcagcac ctgagtatct gcaaaaatat ccgcagccac aatcattaca
    43981 ggaattaagt cgtcatgact gtctggtgac caaagagcgc gatatgaccc acggaatatg
    44041 ggagttgggg aacggtcagg agaaaaaatc ggtgaaggtt agcgggcatc tttcctccaa
    44101 tagcggcgag atagtgttgc aatgggcgct ggagggcaaa gggattatgc tgcgttccga
    44161 gtgggatgtg ttgccgtttc tggagagtgg caaactggtg caagtattgc cggagtatgc
    44221 acaaagcgcc aatatctggg ctgtttaccg ggagccgctc tatcgcagca tgaaattacg
    44281 tgtctgcgtg gaatttctgg cggcatggtg ccagcaacgg ctgggcaagc ccgatgaagg
    44341 ctatcaggtc atgtagatcc accagaaatc actcgggatc cttttcgcgc tttcttttga
    44401 atttcgtcca gatttttgtc tcctgacgac gccacagacg ctggatgttg tcatgatgac
    44461 gcagcaggat caggcaagag agcatcgaaa ccgggaaggt gaattgtggc ttaaaccacc
    44521 atacataaaa cggagcaatc agtgcactga caatcgctcc cagcgacgag tatccgctca
    44581 atagcacggt cagtaaccag gttcccgcca ttactccggt gagatcccag ccaatgggtg
    44641 cgatggcacc aaaagcggta gcaacgcctt ttcctccttt aaatccgaag aaaacgggcc
    44701 agatgtgtcc aagacaggcg gcgatggcaa ttaagcctag ccagaagggg ctgacaccta
    44761 attcatacgc gccccagacg ggcaacattc ctttcagaac gtcgaaaatc agtactgcta
    44821 cggctgctcc cttgccaccg atacgtaaca cattggttgc gcctggattg ccggagccgc
    44881 tggttcgcgg atcgggcagc ccacacaagc ggcaaaccag aatggcactg gaaatggagc
    44941 cgcagaggta cgcgatgagg atcattccag gcgcgattgc actcataagc tgttccgttt
    45001 tgaaaattcg tgttcaacga tgaatctgtg gataatacgc acatttcgcc ggaagtggta
    45061 tccggttagc caaaaagcag gcaggacgtg atggatattg tatttataga gcaactttcg
    45121 gtaatcacca ctattggtgt ttacgactgg gaacagacca tcgaacagaa gttagtgttc
    45181 gatatcgaaa tggcatggga taaccgtaaa gcggcgaaaa gtgatgatgt ggcggattgc
    45241 ctcagttacg ctgacattgc agaaacggtg gtcagccacg tcgagggggc gcgttttgcg
    45301 ctggtggaac gcgtggctga agaggtggcg gagctgctgt tagcacgctt caactcgccg
    45361 tgggtgcgta tcaaactcag caagccaggc gcagtggcgc gggcggcgaa tgttggcgta
    45421 atcattgagc gtggcaataa tctgaaagaa aataattaat tttacagctg ttaaaccaaa
    45481 cggttataac ctggtcatac gcagtagtcc ggacaagcgg tacattttaa taatttaggg
    45541 gtttattgat gagcgatatg tactcgctgc tgatagcggc aatattgggt gtggtcgaag
    45601 gattgacaga atttctgccg gtatccagca cgggccatat gattattgtc ggtcacttgt
    45661 tggggttcga gggcgacacg gcgaaaacct ttgaagttgt gatccagtta ggatcaattc
    45721 tggcggtagt agtgatgttc tggcggcgtc tgtttggcct gattggcatc cactttggcc
    45781 gcccgttgca gcacgaagga gaaagcaaag gtcgtttaac gctgatccac attttgctgg
    45841 ggatgattcc ggcggtggta ctggggctgt tgttccacga cacgattaag tcattgttta
    45901 acccgataaa tgtgatgtat gcactggtcg ttggcggttt gttgctgatt gccgccgaat
    45961 gcctgaagcc gaaagagccg cgtgcgccgg gtcttgatga tatgacctat cgtcaggcgt
    46021 ttatgattgg ctgtttccag tgtctggcgc tgtggcctgg tttctcccgt tccggggcca
    46081 ccatttcagg tgggatgctg atgggcgtga gccgttacgc tgcttccgag ttctcgttcc
    46141 tgctggcggt accgatgatg atgggcgcaa cggcgctcga tctctacaaa agctggggct
    46201 tcctgacaac cggcgatatc ccgatgtttg ccgttgggtt tatcaccgct tttgtggtgg
    46261 cgctgatagc gattaaaacc ttcctgcaat tgattaagcg catttcgttt atcccgttcg
    46321 ccatttatcg ctttattgtg gcggctgcgg tgtacgtcgt gttcttttga ttgagaaaag
    46381 gttcgggagc aggcctggtt ttctccctcg ctctgtaggg agagggccgg ggtgggggtg
    46441 attgttcgca ccggtgctgg tctgttcccc tcaccctaac cctctccccg aaggggcgag
    46501 gggatggttc gtgttaacgt ttagttttat actcagtcag gctttgggca acgttgttcc
    46561 ttccaactgg ctatcgccgc aatccgccgt cgggtcagct cctcgcgaat ctccacacct
    46621 ttaaatcccg cttcaacgac cgcttttgtc ggcactgact gcgccacttc ccaggcttcg
    46681 cgcaaccaac ggccttgcgg gtagtccgct gattcaaaac cggttctgcc gcgcacgtca
    46741 gcctcgctgg tcagcgccag ttgctcgaca cgctgcggtt tacgccaggc atcgatggaa
    46801 tcaaacaatt tgacgatagt ttttgggttc agcattggga aggtgtggat gagatcgtga
    46861 aactcagcca ccagtctggc taaatcgcga atttcatttg gcacgcgcag acgctggcag
    46921 agttgttcca ctaatttcac acccgccgga ccgtgaccat gatgacgcgg ccaaagttct
    46981 ggcggcgtca gtcctttacc gagatcgtgg cataaagttg cgaaacggac atcgacctgc
    47041 ggactcagca tcgccgccat tgagagcgtc attaaggtat gaatacccgt gtcgatttcc
    47101 ggatgccacc tggcaggggc cggaacgcca aacagtgcgt caatttccgg gaataaaacg
    47161 cgcagtgcgc cacaatcgcg cagtacctgg aagaacacct gtggattgcg ggtggtgagg
    47221 gcgctttccg tctctttcca tacccgttca ggcgtcaggt gttccagttc acccgcatgg
    47281 gtcatctcgc gcatcagcgt cagagtttca tcggcaatac gaaaaccgag gtgggcataa
    47341 cgcgcagcaa aacgcgccac gcgcagaacg cgtaacggat cttcgccaaa agcgggggaa
    47401 acatggcgca acagacgatt ttgcagatcg cccagaccgt tgtacgggtc gataatctcg
    47461 ccgttatcgt cctgggccag cgcattaatg gtcagatcgc gacgcttaag atcatcttcc
    47521 agcgtgacat ccggtgcggc atagcaagta aaaccggtgt aaccggaacc ggatttccgt
    47581 tcggtacgtg ccagcgcata ctcttcatgc gtttgcggat gcagaaacac aggaaaatcg
    47641 cggcctacct gctggtagcc cgcgtcgagc atctcctgtg gcgtactgcc gaccaccacc
    47701 caatctctgt ctttgaccgg tagccctaac aatgcatccc gaacagcacc accgaccaga
    47761 taaatcttca cgccatcatc ccttcgtcaa atacacaatt cctaataata agacagtgca
    47821 acgaagaagg cgatttagtt catccagcga tctttgcgtt tgcgcgttgg gatcaggtgt
    47881 ggcagtacca gaccgagcag caagccaagc cccagcacgc caccgccata cataaaccat
    47941 tgcatgatga tggtgcgctg tttgtcatcc aactgtacgc tggcggcatc gacctttttc
    48001 tgcgcgacaa tcagctcgtt tttcaatttc tgattttctt cttttaatcc gttgatcacg
    48061 ctgtcgctct gcgccacttt ttgctgcatt tctgcggtgc gctgattcca ggtgttatcg
    48121 atattggtga gtttatcggt cagggttttg acctgatttt ccagatctgg cacacgggag
    48181 cgcaggcttg gctcagtgct aagttgtttc aacgggatcc aggcggtacg gccagagctg
    48241 tctttcacct gggcataatt ggtgttggcg tcagtttgta ataaggtcac ttcctcgccg
    48301 gcgttaaccg tgcccacgag gcgataatga tctcccggac cgctacggac ccaggtattc
    48361 agttcatcag aaacatagcg cgtttcttca gcgtgtgaga cggcagtggc gctaagtgcg
    48421 agtaaagtta atccgatcag gcgtaatttt ggcatcaggc tgtcgttatt gtcatggaaa
    48481 gtggaacgat agtagtggca tcagtgtcgc tacgcaaagc attcgacatc aatcggaatc
    48541 ctctgtgtca ggtcttgcca ctgcttcaaa cttttacgcc cgtcaaattg cccgttgcat
    48601 ggcaatttgg cgcaaaatac tatctactga caaaaaagat cgcattaagt tcgccgttca
    48661 tcgtcactta tgtgacataa ccgataagta aaggccatgg ctcaggaaat cgaattaaag
    48721 tttattgtta atcacagtgc cgttgaggcg ttgcgtgacc atctcaatac gctgggcggc
    48781 gagcaccatg accccgtgca gttgctgaat atttactacg aaacgccgga taactggctg
    48841 cgtgggcacg atatgggctt acgtattcgt ggcgaaaacg gtcgctatga gatgaccatg
    48901 aaagttgcag gaagagtgac aggcggctta caccagcgcc cggaatataa cgtggcgttg
    48961 agcgaaccga cgctcgacct ggcgcagtta ccgacggaag tctggccgaa cggcgaattg
    49021 cccgccgatc tcgcttcccg cgtacaaccg ctgttcagca ccgattttta tcgcgaaaaa
    49081 tggctggtgg cggtcgatga cagccgaatt gaaatcgctc tcgatcaggg ggaggtgaaa
    49141 gcgggggaat ttgctgaacc tatctgtgag ctggaactgg aactgcttag cggcgacacg
    49201 cgcgcggtgc tgaaactggc gaaccaactg gtatcgcaaa ccggattacg ccagggcagc
    49261 ctgagcaaag cggcgcgtgg ctatcatctg gcgcagggca atccggcgcg tgaaatcaaa
    49321 ccgaccacca ttttgcatgt tgcggcaaaa gccgatgtgg aacaggggct ggaagcggcg
    49381 ttcgagctgg cgttagcgca atggcagtat catgaagaac tgtgggtacg cggcaacgat
    49441 gcggcgaaag agcaggtgct ggcagccatt ggccttgttc gccatgcact gatgctgttc
    49501 ggtggcattg tgccgcgtaa agcgagcact cacttacgtg atttgctgac tcaatgcgaa
    49561 gcgaccatcg cttctgcggt ttctgccgtg acggcggtct actctaccga aacggcaatg
    49621 gcaaagctgg cgttgaccga atggttggta agcaaagcct ggcagccgtt tttagatgcc
    49681 aaagcgcagg gtaaaatcag cgattccttc aaacgctttg ccgatatcca tctttcccgc
    49741 catgccgctg aactgaaaag cgttttctgc cagccgttag gcgatcgcta ccatgatcag
    49801 ttgccacgcc tgacgcgtga tattgactca atactgctgc tggcaggtta ctatgatcct
    49861 gtcgtcgcgc aagcctggct ggagaactgg caggggctgc gtcacgctat tgcgaccggg
    49921 caacgcattg aaattgaaca tttccgtaat gaggcaaaca atcaggaacc gttctggttg
    49981 cacagcggaa aacgttaatc aggcaaggat aaaatcgctt atgaagccgc tctcttcacc
    50041 gttacagcag tactggcaga ccgttgttga gcggctgcca gagcctttag ccgaggaatc
    50101 acttagcgca caggcgaagt cagtacttac ttttagtgat tttgtgcagg acagcgtgat
    50161 tgtgcatcca gagtggctga cggaactgga aagccaaccg ccgcaggccg acgaatggca
    50221 gcattacgcg gcatggttgc aggaggcgct cagtaatgtg agtgacgaag ccgggttaat
    50281 gcgcgagctg cggctattcc ggcggcgcat tatggtgcgc atcgcctggg cgcaaacgct
    50341 ggctttggtt actgaagaga gcatattgca gcagctcagc catctggcgg agacgctgat
    50401 tgtcgccgcg cgtgactggc tgtatgacgc ttgctgccgc gagtggggaa cgccgtgcaa
    50461 tgcgcagggc gaagcgcaac cgctgctgat tttaggcatg ggtaagctgg gcggtgggga
    50521 actgaatttc tcctcagata tcgatctgat ttttgcctgg ccggaacatg gttgtacgca
    50581 gggcggacgc cgggaactgg ataacgcgca gttttttacc cgcatggggc agcggctgat
    50641 taaagtgttg gatcaaccaa cgcaggatgg cttcgtctat cgcgtggata tgcggctgcg
    50701 tccgtttggc gaaagtggcc cgctggtgct gagctttgcc gcgttggagg attattacca
    50761 ggagcagggg cgcgactggg agcgttacgc gatggtcaag gcgcggatta tgggcgatag
    50821 cgaaggcgtc tatgctaacg agttgcgtgc gatgctgcgc ccgtttgttt tccgtcgtta
    50881 catcgatttc agcgtgattc agtcgctgcg caacatgaaa gggatgattg cccgtgaagt
    50941 gcgtcgacgt ggtttgaccg acaatatcaa actcggcgca ggcggcattc gcgaaattga
    51001 atttatcgtt caggtgttcc agctcattcg cggtgggcgc gaaccgtcgc tgcagtcgcg
    51061 ctctttactg ccaacgctca gcgtcattgc cgcgctgcat ctgctttctg aaaacgatgc
    51121 tgaacaattg cgagtggcgt atctgttcct gcggcgtctg gaaaacctgc tgcaaagcat
    51181 taacgacgaa caaacccaga cgcttccttc tgatgagctt aaccgtgcgc ggctggcgtg
    51241 ggcgatggac tttgctgact ggccgcaact gaccggggcg ctgaccgcac atatgaccaa
    51301 tgtgcgccgg gtgtttaatg aattgattgg cgacgacgaa agtgaaactc aggaagagtc
    51361 gctgtcggaa cagtggcgtg agctgtggca ggatgcgttg caggaagatg acactacgcc
    51421 agtgctggcg catcttagcg aggatgatcg caaacaggtg ctaacgctga ttgccgattt
    51481 ccgcaaagag ctggataagc gcaccatcgg gccgcgagga cgtcaggtgc tcgaccatct
    51541 gatgccgcat ctgctaagtg atgtctgtgc gcgtgaagac gctgccgtta cgctgtcgcg
    51601 cattaccgcc ttgctggtgg ggattgttac ccgcaccacc tatttagaat tgctcagtga
    51661 attccccgcg gcgcttaaac atttgatttc tctgtgtgcc gcgtcgccga tgattgccag
    51721 ccaactggcg cgttatccat tattgctgga tgaattgctc gatccaaaca ccctttacca
    51781 gccgacggcg accgatgcct accgcgatga gttgcgccag tatttgctgc gcgtgccgga
    51841 agatgacgaa gagcaacagc ttgaggcgct gcgtcagttc aaacaggcgc agctgttacg
    51901 catcgccgca gcggatatcg ctggtacgct accggtgatg aaagtgagcg atcacttaac
    51961 ctggctggca gaggctatga tagatgccgt cgttcagcag gcgtgggttc aaatggtggc
    52021 ccgctacggt aagccaaatc acctcaacga tcgcgaaggc cgtggttttg cggtggtcgg
    52081 ctacggcaag ctgggcggct gggagttagg ctacagttcc gatcttgacc tgatcttcct
    52141 ccatgattgc ccaatggatg cgatgactga cggcgagcgg gaaatcgacg ggcgacagtt
    52201 ttatctgcgt ctggcgcaac gcattatgca tctgttcagt acgcgtacct cttccggcat
    52261 tttgtatgaa gtggatgcac ggctacgccc gtccggggcc gcgggaatgc tggtgacatc
    52321 cgcagaagca tttgccgatt atcagaaaaa cgaggcctgg acgtgggaac atcaggcgct
    52381 ggtgcgtgcg cgggtagtgt acggcgatcc gcagctcacc gcgcactttg acgcggtgcg
    52441 tcgcgagatt atgacgctgc cgcgtgaagg taaaactctg caaacggaag tgcgggaaat
    52501 gcgcgagaaa atgcgcgctc atctcggcaa taaacatcgc gatcgctttg atatcaaagc
    52561 tgatgaaggc ggaattaccg atatcgaatt tattacccaa tatctggtgt tgcgctacgc
    52621 tcatgaaaaa ccgaagttaa cgcgctggtc agacaacgtg cgcattctgg aactactggc
    52681 gcaaaacgac attatggaag agcaggaagc gatggcgctg acccgtgctt atactacgct
    52741 gcgcgatgaa cttcatcatc tggcattaca ggaattgccg ggccatgtgc cggaggattg
    52801 cttcaccgca gagcgtgaac tggtgcgggc aagctggcag aagtggctgg tggaagaatg
    52861 aagtatggta ttatcgcgcg caaattttga atctctcagg agacaggaat gaaagtaacg
    52921 ctgccagagt ttgaacgtgc aggagtgatg gtggttggtg atgtgatgct ggatcgttac
    52981 tggtacggtc ccaccagccg tatctcgccg gaagcgccgg tgcccgtggt taaagtcaat
    53041 accatcgaag aacgtccggg cggcgcggct aacgtggcaa tgaacatcgc ttctctcggt
    53101 gccaatgctc gcctggtcgg gctgacgggc attgacgatg cggcgcgtgc gctgagtaaa
    53161 tctctggccg acgtcaacgt caaatgcgac ttcgtttctg taccgacgca tccgactatc
    53221 accaaattac gggtactttc ccgcaaccaa cagctgatcc gcctggattt tgaagaaggt
    53281 ttcgaaggtg ttgatccgca gccgctgcac gagcggatta atcaggcgct gagttcgatt
    53341 ggcgcgctgg tgctttctga ctacgccaaa ggtgcgctgg caagcgtaca gcagatgatc
    53401 caactggcgc gtaaagcggg cgttccggtg ctgattgatc caaaaggtac cgattttgag
    53461 cgctaccgcg gcgctacgct gttaacgccg aatctctcgg aatttgaagc tgttgtcggt
    53521 aaatgtaaga ccgaagaaga gattgttgag cgcggcatga aactgattgc cgattacgaa
    53581 ctctcggctc tgttagtgac ccgttccgaa cagggtatgt cgctgctgca accgggtaaa
    53641 gcgccgctgc atatgccaac ccaggcgcag gaagtgtatg acgttaccgg tgcgggcgac
    53701 acggtgattg gcgtcctggc ggcaacgctg gcagcgggta attcgctgga agaagcctgc
    53761 ttctttgcca atgcggcggc tggcgtggtg gtcggcaaac tgggaacctc cacggtttcg
    53821 ccgatcgagc tggaaaatgc agtgcgtggg cgcgcagata ccggctttgg tgtgatgacc
    53881 gaagaggaac tgaagcttgc tgtagcggca gcgcgtaaac gcggcgaaaa ggtggtaatg
    53941 accaacggtg tctttgacat cctgcacgcc gggcacgtct cctatctggc aaatgctcgc
    54001 aaactgggtg accgtctgat tgtcgctgtg aacagcgatg cctccaccaa acggctgaaa
    54061 ggggattccc gcccggtaaa cccactcgag cagcgtatga ttgtgctggg cgcactggaa
    54121 gcggtcgact gggtagtgtc gtttgaagaa gacacgccgc agcgtctgat cgccgggatc
    54181 ctgccagatc tgctggtgaa aggcggcgac tataaaccag aagaaattgc cgggagtaaa
    54241 gaagtctggg ccaacggcgg cgaagtgctg gtgctcaact tcgaagacgg ttgctcgacc
    54301 actaacatca tcaagaagat ccaacaggat aaaaaaggct aaccggaaag cggttcacag
    54361 atctcgcttc gtagcaggca ggcctattga cgctgttgtt ttttcaacct aaagtaaagg
    54421 aacaagggta agggaggatt tctcccccct ctgatcagtt gttagtaagt cgggaaactt
    54481 aacagtaaca acacaaccag tatgatgacg aacttcatca taaccctttc cttctataag
    54541 gccccttcct cgggaggggc tttcccgttt cagccccttg ctgactcccc gattgttgat
    54601 gagcgccgga ccccgcgcaa cgctttccct gaagcagttt tgccttcccc gagcggtcac
    54661 gcaaaacgct gcaacgtcct gagcatttgc accttttagc ggaacgcaga tataaaaaat
    54721 ccccggcttt ttgggccagg gatatgtgtt aatgaaacct tcttgacgct gatttttttt
    54781 caacctaaag taaaggaaca agggtgaggg aggatttctc ccccctctga ttggctgtta
    54841 ataagctgcg aaacttacga gtaacaacac aatcagtatg atgacgagct tcatcataac
    54901 cctttccctc tgtaaggccc cttcctcggg aggggctttc ccgtttcagc cctttgctga
    54961 ctccccgatt gttgatgagc gccggacctc gcgcaacgct ttcccagagg cagttttgcc
    55021 ttccccgagc ggtcacgcaa aacgttgcaa cgacctgatc atttgcagtt tttagcggaa
    55081 cgcagatata aaaaatcccc ggctttttgt gccagggacg ttcatagctg aaaatttatt
    55141 ctacgttttc ggcgacggga gttgttgagg ttaagggtga agtcaatgcc gccagtgagc
    55201 cgcctgaaac gccaatttca ttgagcaagg agtcaatcag cggtgcctgt gtgcggtaag
    55261 agagagcggc tgataatgct tgttccgcca ggtttccgcc ccccacatta cccgtgtttg
    55321 catcacccgc agcgctgcca cgattcagac catcgacctg aataatcttg ataccgtcaa
    55381 tagatttcat cggctcaacg gatttttcta ttaccgcagg tagcgcctgc aacaaggcca
    55441 gtttgaattt aaggctggtt tgttcatcag aaagtacgtt gatagcatcg ttcagcgcac
    55501 gttgtgcttc tgcttccgcc agaccctttt tacgtgtagc ttcggctaac tcaacgatag
    55561 ccgccgcctg catctctgcg gcttcttttt ctgctttcgc ccgcacggtc agttcaaccg
    55621 ctttggtttc tgcatcctgc gcggcagcga ttagggcaac ttgtttagca cgatcggctt
    55681 ctgcggtctg gcgagtggtt tctacgtttt gttgcgcact tactgcttct gcgagtgcaa
    55741 ggttagcacg cgcttctgcc tgggactgtt gttccgattt ggcggcaata gcgatcgatt
    55801 tcgtctggtt ggcgatttcg gtgacctgct gttgttcgat ctctttaata cgaacttcac
    55861 gttcagcctc aacctttctt gagcggacgg cctgctcgcg ctcgatctcc gtttcctgaa
    55921 tttgtcgttc agccagaatt cgcgtctgct ctgcttcacg acgacgttca gcttcaaaag
    55981 ccgcaatacg tgcattctgt tcggcagtac gggttttaac ctgctgctcc tgctcaagcg
    56041 tcataaacgc ttcttgttgt tcaatctcca acttgcgcga aagtgcatca cggtttttct
    56101 cacgcaccgc aacttctaca tcctgttcaa cttcgttacg ttcgcgacga cggcgctccg
    56161 tctcctgagt cagtttggtt aaaccttcgg cgtcaaaggc attgttcgga ttgaaatgtt
    56221 ctttcgaggt ctggttaaag ttggtaagtg aaacgctttc cagctcaaga ccgtttttcg
    56281 acaggtcttc tgcgacagta ttttgtactc cctgcacgaa gttctcgcgg gtatcctgta
    56341 actcatgcat ggtcatttgt gcagctgttg cacggagggc atcgacaaat ttatcttcaa
    56401 ccaacatacg taagtcttct ggtgatagcg tgcgttgccc caacgtctgc gccgcagtgg
    56461 cgatgccttc tacagacggt tttacccgta caaagaaagc gactactaca tcaacacgca
    56521 tacgatcttt cgtaatcagg ctatcaatgg ttgagcggct gacttccagc ttcagagtat
    56581 tcatattgat ggggattatt tcatgaaaga tcggcatcac gattgcgcca ccgctcatca
    56641 ccactttttg cccacctaaa ccagtacgaa caaaagcttg ctctgccgaa gcgcgacgat
    56701 agagcctggc gaaaataatt ccaataataa acagaatgca tacggcaata atcgcggtaa
    56761 acatccagga gggcacagaa ttaacaatat catccattat taaatttcct tttgattaat
    56821 aaattacaat atttgtggcc agggattgtt ttccgccaaa tatcgcgttg cagaaagtcg
    56881 gcaaataatt aacactttgt ctcctttagt gaaaaatttt ccttcttcag gctccagtag
    56941 taaataatgt atttggccaa attgatcggt gagttttcct tcgcagggat tacctgaagt
    57001 ggcttgatga ccggtaatta atgccatgct accaatatat tcttcttctg taatagctga
    57061 actatggtcg cgtggaatcc agggcgcaac tattttaccg gtataatgca ccgcaataat
    57121 cgtaaacagt aaactgacag gaaccacgaa caggttcgac agtggtgatt gccagaccat
    57181 gatgcaggca tgctgcaaca ggatgccaat aagaccaaaa aaaccagcca gcaagcagag
    57241 aacgacgaga gctggcaatc ttccgatatt gagataatga agtgcctggc taatatgacc
    57301 tgtggttatg gaatcataat ggtcaagatg tgcatcaaga gcaccggaga gcatgtgacc
    57361 gcagataagc gctaaaattt ctaaaaggcc gatcaacagt acaaaggaaa tagcgaaaag
    57421 ataaggggta ttatagtcgg cgaataaaat catatgtcac tccaatgtcc atttagtgac
    57481 gcaatattta ctttgggata gtttccttgc ttacatttat ctttgttaat aataacacat
    57541 gttagagaag ttctggaaga gaaagttttt taatatggtt ttttattttt cactaaaaag
    57601 tgtgatcggg gacaatatat ttacgcacgt tatgtttaaa ggcactacac tgattgggga
    57661 atactgaaat cagaaaaagc atatggcctt aaaaggtatg cgataaccgg aggatgtgct
    57721 tatggatcat agtcttaatt ctttaaataa tttcgatttc ctggcgcgta gttttgccag
    57781 aatgcacgca gaaggtcgcc cggtcgatat tcttgccgtt actggtaaca tggatgaaga
    57841 acatagaacc tggttttgcg cacgttatgc ctggtattgt caacagatga tgcaggcaag
    57901 agagctggag ttagagcact gatataacgg gcctgatggc ccgttttagt gtttgtatta
    57961 gatgctagtt ataggtgatt ttgaggatga cgtctttact aaacggtccc tcaacaacag
    58021 tctctgccgg attcgcacta agttctgccc tgaatggtgc ggtgaccgaa ggtgcactag
    58081 acgatgaaaa agaagcaaat tgtgagaagc ggttaaattc cgttgctgtt ccatctttat
    58141 gaaatatctt caatagcagt ccattaccca tatccagatg gctataatca ctcaaaatat
    58201 ccgtagtgaa aaagctccct aaaatattga attgctcagt acaggccgca ccaggatctt
    58261 ttgtcatgac tacactaaac gtctcactaa cgctggtatt tttaattgtt cgggaattta
    58321 ttttaccaaa gtcaacaacg ccatcagctg gttccagtgc gacattaaca aagcagtcaa
    58381 ggatatgaat attatttaat ccacgaatcc tgaaggtcaa atttttgtct acttctatag
    58441 ctcctgcgcc actcatacct ttaaactcta gagcgatata ttcatcaggt aacaccaggt
    58501 ttttattaac tctattggca ttttttgcta atttaatata gagttgtacg cgagctggaa
    58561 aggttactga taattgaggc tcaggacctc cactacagac agcggggttt ttatagtgat
    58621 tattaaaggt acccgctcct tttatatcaa tgcattctgg tgctgcgata gcattagggg
    58681 cagtcgaatc ataatcaact ccattgtatg ttacgccaaa aatataggag ttctgattat
    58741 aaaagtcctg accgcctggt aaaaacatca ttatattagt ccaggctgat gggtattctt
    58801 ctccaggtga tggggcaggt aatgcat
//
LOCUS       DAFAVF010000011        58389 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFAVF010000011 DAFAVF010000000
VERSION     DAFAVF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.011"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(99..3248)
                     /locus_tag="LGK42_000654"
     CDS             complement(99..3248)
                     /locus_tag="LGK42_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBK2656934.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="LGK42_000655"
     CDS             complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="LGK42_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBK2656935.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4606..5253
                     /gene="acrR"
                     /locus_tag="LGK42_000656"
     CDS             4606..5253
                     /gene="acrR"
                     /locus_tag="LGK42_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBK2656936.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5381..8743
                     /gene="mscK"
                     /locus_tag="LGK42_000657"
     CDS             5381..8743
                     /gene="mscK"
                     /locus_tag="LGK42_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBK2656937.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8955..9116)
                     /gene="rsmS"
                     /locus_tag="LGK42_000658"
     CDS             complement(8955..9116)
                     /gene="rsmS"
                     /locus_tag="LGK42_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBK2656938.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9130..9657)
                     /gene="priC"
                     /locus_tag="LGK42_000659"
     CDS             complement(9130..9657)
                     /gene="priC"
                     /locus_tag="LGK42_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBK2656939.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9727..10104
                     /locus_tag="LGK42_000660"
     CDS             9727..10104
                     /locus_tag="LGK42_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBK2656940.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10257..10808
                     /gene="apt"
                     /locus_tag="LGK42_000661"
     CDS             10257..10808
                     /gene="apt"
                     /locus_tag="LGK42_000661"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK2656941.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10937..12868
                     /gene="dnaX"
                     /locus_tag="LGK42_000662"
     CDS             10937..12868
                     /gene="dnaX"
                     /locus_tag="LGK42_000662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK2656942.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12201..12265
                     /gene="dnaX"
                     /locus_tag="LGK42_000662"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12921..13250
                     /locus_tag="LGK42_000663"
     CDS             12921..13250
                     /locus_tag="LGK42_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK2656943.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13250..13855
                     /gene="recR"
                     /locus_tag="LGK42_000664"
     CDS             13250..13855
                     /gene="recR"
                     /locus_tag="LGK42_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK2656944.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13965..15839
                     /gene="htpG"
                     /locus_tag="LGK42_000665"
     CDS             13965..15839
                     /gene="htpG"
                     /locus_tag="LGK42_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBK2656945.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16020..16664
                     /gene="adk"
                     /locus_tag="LGK42_000666"
     CDS             16020..16664
                     /gene="adk"
                     /locus_tag="LGK42_000666"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK2656946.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16900..17862
                     /gene="hemH"
                     /locus_tag="LGK42_000667"
     CDS             16900..17862
                     /gene="hemH"
                     /locus_tag="LGK42_000667"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBK2656947.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17859..18818)
                     /gene="aes"
                     /locus_tag="LGK42_000668"
     CDS             complement(17859..18818)
                     /gene="aes"
                     /locus_tag="LGK42_000668"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBK2656948.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18970..20274
                     /gene="gsk"
                     /locus_tag="LGK42_000669"
     CDS             18970..20274
                     /gene="gsk"
                     /locus_tag="LGK42_000669"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBK2656949.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20357..22033)
                     /locus_tag="LGK42_000670"
     CDS             complement(20357..22033)
                     /locus_tag="LGK42_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBK2656950.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22271..23491)
                     /gene="fsr"
                     /locus_tag="LGK42_000671"
     CDS             complement(22271..23491)
                     /gene="fsr"
                     /locus_tag="LGK42_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBK2656951.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23709..25361
                     /gene="ushA"
                     /locus_tag="LGK42_000672"
     CDS             23709..25361
                     /gene="ushA"
                     /locus_tag="LGK42_000672"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBK2656952.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25398..25877)
                     /gene="ybaK"
                     /locus_tag="LGK42_000673"
     CDS             complement(25398..25877)
                     /gene="ybaK"
                     /locus_tag="LGK42_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBK2656953.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26081..26875)
                     /locus_tag="LGK42_000674"
     CDS             complement(26081..26875)
                     /locus_tag="LGK42_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBK2656954.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27013..27354
                     /locus_tag="LGK42_000675"
     CDS             27013..27354
                     /locus_tag="LGK42_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBK2656955.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27468..29972)
                     /gene="copA"
                     /locus_tag="LGK42_000676"
     CDS             complement(27468..29972)
                     /gene="copA"
                     /locus_tag="LGK42_000676"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBK2656956.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30234..31166
                     /gene="glsA"
                     /locus_tag="LGK42_000677"
     CDS             30234..31166
                     /gene="glsA"
                     /locus_tag="LGK42_000677"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK2656957.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGEWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31169..32461
                     /locus_tag="LGK42_000678"
     CDS             31169..32461
                     /locus_tag="LGK42_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2656958.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32586..32993
                     /gene="cueR"
                     /locus_tag="LGK42_000679"
     CDS             32586..32993
                     /gene="cueR"
                     /locus_tag="LGK42_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBK2656959.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(32994..33452)
                     /locus_tag="LGK42_000680"
     CDS             complement(32994..33452)
                     /locus_tag="LGK42_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK2656960.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33449..34366)
                     /locus_tag="LGK42_000681"
     CDS             complement(33449..34366)
                     /locus_tag="LGK42_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBK2656961.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34512..35189
                     /gene="fetA"
                     /locus_tag="LGK42_000682"
     CDS             34512..35189
                     /gene="fetA"
                     /locus_tag="LGK42_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBK2656962.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35176..35955
                     /gene="fetB"
                     /locus_tag="LGK42_000683"
     CDS             35176..35955
                     /gene="fetB"
                     /locus_tag="LGK42_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBK2656963.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36018..36872)
                     /gene="cnoX"
                     /locus_tag="LGK42_000684"
     CDS             complement(36018..36872)
                     /gene="cnoX"
                     /locus_tag="LGK42_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBK2656964.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(36933..37742)
                     /gene="ybbO"
                     /locus_tag="LGK42_000685"
     CDS             complement(36933..37742)
                     /gene="ybbO"
                     /locus_tag="LGK42_000685"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBK2656965.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTIRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37732..38355)
                     /gene="tesA"
                     /locus_tag="LGK42_000686"
     CDS             complement(37732..38355)
                     /gene="tesA"
                     /locus_tag="LGK42_000686"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBK2656966.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38326..39012
                     /locus_tag="LGK42_000687"
     CDS             38326..39012
                     /locus_tag="LGK42_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2656967.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPTQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39009..41423
                     /locus_tag="LGK42_000688"
     CDS             39009..41423
                     /locus_tag="LGK42_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2656968.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGTIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(42136..43230)
                     /gene="mnmH"
                     /locus_tag="LGK42_000689"
     CDS             complement(42136..43230)
                     /gene="mnmH"
                     /locus_tag="LGK42_000689"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBK2656969.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(43299..44225)
                     /gene="allS"
                     /locus_tag="LGK42_000690"
     CDS             complement(43299..44225)
                     /gene="allS"
                     /locus_tag="LGK42_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBK2656970.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            44455..44937
                     /gene="allA"
                     /locus_tag="LGK42_000691"
     CDS             44455..44937
                     /gene="allA"
                     /locus_tag="LGK42_000691"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBK2656971.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45015..45830
                     /gene="allR"
                     /locus_tag="LGK42_000692"
     CDS             45015..45830
                     /gene="allR"
                     /locus_tag="LGK42_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBK2656972.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45920..47701
                     /gene="gcl"
                     /locus_tag="LGK42_000693"
     CDS             45920..47701
                     /gene="gcl"
                     /locus_tag="LGK42_000693"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBK2656973.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPSFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47714..48490
                     /gene="hyi"
                     /locus_tag="LGK42_000694"
     CDS             47714..48490
                     /gene="hyi"
                     /locus_tag="LGK42_000694"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBK2656974.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            48590..49468
                     /gene="glxR"
                     /locus_tag="LGK42_000695"
     CDS             48590..49468
                     /gene="glxR"
                     /locus_tag="LGK42_000695"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBK2656975.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            49637..51091
                     /locus_tag="LGK42_000696"
     CDS             49637..51091
                     /locus_tag="LGK42_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBK2656976.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            51151..52512
                     /gene="allB"
                     /locus_tag="LGK42_000697"
     CDS             51151..52512
                     /gene="allB"
                     /locus_tag="LGK42_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBK2656977.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            52569..53870
                     /locus_tag="LGK42_000698"
     CDS             52569..53870
                     /locus_tag="LGK42_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBK2656978.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            53892..55037
                     /gene="glxK"
                     /locus_tag="LGK42_000699"
     CDS             53892..55037
                     /gene="glxK"
                     /locus_tag="LGK42_000699"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBK2656979.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(55166..55951)
                     /gene="allE"
                     /locus_tag="LGK42_000700"
     CDS             complement(55166..55951)
                     /gene="allE"
                     /locus_tag="LGK42_000700"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBK2656980.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(55962..57197)
                     /gene="allC"
                     /locus_tag="LGK42_000701"
     CDS             complement(55962..57197)
                     /gene="allC"
                     /locus_tag="LGK42_000701"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBK2656981.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(57219..58268)
                     /gene="allD"
                     /locus_tag="LGK42_000702"
     CDS             complement(57219..58268)
                     /gene="allD"
                     /locus_tag="LGK42_000702"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBK2656982.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
BASE COUNT        13869 a        15017 c        15486 g        14017 t
ORIGIN      
        1 cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt tatgcataaa
       61 aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc gacagtatgg
      121 ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccacaac aaagaatacc
      181 ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac accggtacct
      241 actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat aacgccgagg
      301 ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc atcaagcgtc
      361 gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc gacgataagt
      421 atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacctggaa gtaaacgtca
      481 ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag cggaacgacc
      541 agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgccagaca caggaatacg
      601 acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagaggcg ttcctgatag
      661 gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag ttgttccatt
      721 agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat ttccatggat
      781 ggcaggccat tgtaacgttc cagacgcggc gaaccgtact cccaacgaga agaggagaat
      841 gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc gatatcatcc
      901 ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg accgcggtcg
      961 ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt aatgtcgttg
     1021 atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa ctgcggggta
     1081 tcttccagac cgtttggacg tacgctggtc agcatatcag ggtgcttcgc tgcttctgca
     1141 agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc
     1201 tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt aaaggcgaaa
     1261 accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat cgcttcaact
     1321 ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc aataccggta
     1381 ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc aacgttgttc
     1441 ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt acgttcctgc
     1501 gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc atctggcaag
     1561 aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat cagatacagc
     1621 accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta gtggtgcgtg
     1681 ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc cccgtgatcg
     1741 cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat caacgctacc
     1801 agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata gatagcacca
     1861 gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat cgcgataccg
     1921 accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg cggcaaacct
     1981 tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc caccaacaag
     2041 ccgatagcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc aaaggcggca
     2101 aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat caacgtcgcg
     2161 cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc ttcgaccagc
     2221 gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta cgggtaaaca
     2281 attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg gattgccgca
     2341 gcggtatcca gcgcgtttgc accggtcgcc agcttgatcc ccagaccgga agccggttgg
     2401 ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt cgccacatca
     2461 cgcagcagta cgcgggaacc atcttgattc actttcagca ggattttgcc gaactcttca
     2521 gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc tttcaccggc
     2581 ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat ggcggtaatg
     2641 acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca gatacgcatc
     2701 gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct gatggcatct
     2761 ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc atcggtgttg
     2821 ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac cccttgctgc
     2881 tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc
     2941 gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt acccgtggag
     3001 tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg ttcgataacc
     3061 tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc ggagatcgtt
     3121 actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat cgccagcccc
     3181 cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc gataaagaaa
     3241 ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag gctgagcacc
     3301 gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct ggacaccagg
     3361 acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac cttctgtcac
     3421 cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca ctttgtcatc
     3481 cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc cctgttgcgg
     3541 gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga acatacccgg
     3601 cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc cagtggtctg
     3661 atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa tgccgtcact
     3721 ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc
     3781 ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag
     3841 ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc cttccgtcac
     3901 gttcgactta ccaatgcgac cgctaatcgg agaggtgact ttggtgtaag ccagattgat
     3961 ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct gttgcgcatc
     4021 agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca gtttctgata
     4081 acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca gatcaccttt
     4141 cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag agacacctgc
     4201 ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa cttgaggacg
     4261 aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga tctgcagagg
     4321 ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt gttgggcctg
     4381 tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga ccgccagagg
     4441 cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca aattggtcaa
     4501 tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta tggtacatac
     4561 attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc acgaaaaacc
     4621 aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg tcttttctca
     4681 cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg cgttacgcgc
     4741 ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat ctgggaactg
     4801 tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc tggcgatcca
     4861 ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac agaagaacga
     4921 cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga aatggctgtt
     4981 gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga acaaacgtta
     5041 aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg ggcagcaatt
     5101 attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc gcaatctttt
     5161 gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta tctcctgtgc
     5221 cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca ggatttttcc
     5281 tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg cggtttgact
     5341 ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt tccagtatta
     5401 caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg tcttgttatg
     5461 ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa aagcggacct
     5521 gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg acaaactggt
     5581 gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgca taaaagaaga
     5641 gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc aggcgaccgc
     5701 ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa ttctgagtac
     5761 gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt tgcaaaacgc
     5821 acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc agcccgaacg
     5881 cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca gtcgtctgga
     5941 tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa tgcaggctca
     6001 gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag ggaacaccgt
     6061 cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg cccgtctgga
     6121 gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt taactgaaaa
     6181 aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta atccgctggt
     6241 gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg cgactgaaaa
     6301 cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc gggcgctgca
     6361 atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc tgttgtctcg
     6421 tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa acatgaccaa
     6481 ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg atgcactctt
     6541 ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag tcaacagcga
     6601 agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg atcaactcaa
     6661 caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc agcagcagtt
     6721 aatgagcgta tcgaaaaacc tgaaatccat cctgactcag caaatctttt gggtgaacag
     6781 taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga aagatgaatt
     6841 taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt ttatcgcttt
     6901 cctcgctggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc tgggctggct
     6961 gaaagcgtat caacagaaac tggcttccgc tgtgggttcc ctgcgtaacg atagccagct
     7021 caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt gcctgattat
     7081 tctcgcggtt ggcctgattc tgttgaccat gcagctcaac atcagcgaac tgctatggtc
     7141 gttcagtaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga aggtactgga
     7201 gaaaaatggc gttgccgtgc gtcacttcgg catgccggag cagcagacca gccactggcg
     7261 tcggcaaatt gtccgcatca gtctcgcgtt gctgcctatc catttctggt ctgtggtggc
     7321 agaactttcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga ttttcttcaa
     7381 cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc gtgataaaga
     7441 gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga ttgcattgat
     7501 ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc gttggattga
     7561 aaccgtttat ctggtgatca tctggaactt gctctaccag acggtattgc gcggcttaag
     7621 cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga atctggtgaa
     7681 agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg agcaagttaa
     7741 ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg tcatgttctg
     7801 ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca cgctctggca
     7861 ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg gcagtctgtt
     7921 gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc ctggtttact
     7981 ggaagtgctg gtgctctcgc gactgaatat gcgtcagggc gcgtcgtatg ccattactac
     8041 catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat cgctgggcgt
     8101 ctcttgggat aaactccagt ggctggccgc agcattatcc gtaggtcttg gttttggttt
     8161 acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac gtccggtgcg
     8221 tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga tccgtattcg
     8281 tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca aagcgtttgt
     8341 taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg tgatccgtct
     8401 cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga aggcggcgac
     8461 tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg cgtttggtgc
     8521 cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc gtagtcgtac
     8581 tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca tcaacattgc
     8641 ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga cggaagtcaa
     8701 acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa agggggcgac
     8761 atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat gcggcgtgac
     8821 cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg gtgtaggcct
     8881 gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg acatttagcg
     8941 gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt ttcataatcg
     9001 cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt ttcttccagc
     9061 agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag tgacatactc
     9121 tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca gcgcatggcg
     9181 gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt gctgttccac
     9241 gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac gtagccgccg
     9301 ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg gtggcgcact
     9361 gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca gttgtgccgc
     9421 cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg catgacgaag
     9481 cgcagccaga ttatcgcccg cctcatcgag acaagcctgc agtgttgtcg cacgggtttg
     9541 aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg gggcacaacg
     9601 ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg ttttcacgat
     9661 aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa gctgacaaca
     9721 ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt gctgggtacg
     9781 ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc ggcctggtgc
     9841 tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg gtttggcagc
     9901 tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc gcgggcgatt
     9961 ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc atgggtgcgc
    10021 atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat tccggtgatt
    10081 gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat tgcgtacagc
    10141 cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa caactgttac
    10201 ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat acacttatga
    10261 ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt caggactacc
    10321 caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg aaagcttacg
    10381 ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc aaagttgtcg
    10441 gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg ggcgttggct
    10501 ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa acttacgacc
    10561 tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg ggcgacaaag
    10621 ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc gttaaactga
    10681 tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg ttcgatctcg
    10741 gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc ccgttcccgg
    10801 gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag atgtgcattc
    10861 agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc accttccagc
    10921 gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc cacaaacctt
    10981 tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct tgtcgttagg
    11041 gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa cctctatcgc
    11101 ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc cgtgcggcgt
    11161 gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc
    11221 cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc agtacgctcc
    11281 ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt cgcgccacag
    11341 ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt ttctgctggc
    11401 gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc aatttcatct
    11461 caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca acgaagaaca
    11521 tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag gcagcctgcg
    11581 agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg tttcaaccca
    11641 ggcagtcagt gcgatgctgg gtacgcttga cgacgatcag gcgctgtcgc tggttgaagc
    11701 gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg ctgcccgtgg
    11761 tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta ttgcgatggt
    11821 acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc ggatgcgtga
    11881 actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc tgttgattgg
    11941 tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga cgctgctgcg
    12001 cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccac gccagtcctt
    12061 tgcgcccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc aaccgcaatc
    12121 agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc
    12181 gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc
    12241 cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa agactggctt cggtcaccga
    12301 tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc
    12361 gtatcgctgg aaggctacca ctccagtgat gcagcaaaaa gaagtggtcg ccacgccgaa
    12421 ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga agctggcggc
    12481 agaagccatt gagcgcgacg cgtgggcggc acaggtgagt caactttcgc taccaaaact
    12541 ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca
    12601 tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc
    12661 gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc
    12721 ggtgcgtacg ccgctggagt ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg
    12781 cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg atgcggagct
    12841 ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa
    12901 cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag
    12961 cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtca
    13021 ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc
    13081 gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg
    13141 ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct
    13201 ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag
    13261 cccgctgtta acacagctta tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc
    13321 ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc
    13381 gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac
    13441 cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg
    13501 tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt tttcaggccg
    13561 ttattttgtg ttgatggggc acctgtcacc gctggacggc atcggtccgg atgatatcgg
    13621 ccttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac
    13681 caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata
    13741 tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt
    13801 cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt tttaagcaaa
    13861 cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc
    13921 atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa
    13981 ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt
    14041 ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc
    14101 tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc
    14161 gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga
    14221 cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc
    14281 tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag tttggtgttg
    14341 gtttctactc tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg
    14401 aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg
    14461 acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg
    14521 agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc
    14581 tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc tcctgggaga
    14641 aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt
    14701 acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc tggagccaca
    14761 accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt
    14821 gggatatgtg gaaccgtgat cataaacacg gcctgaaact gtacgtccag cgtgtgttca
    14881 tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg
    14941 actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc
    15001 gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag
    15061 acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc
    15121 cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctactt
    15181 ataccgattc ttctgcgcag accgtgtctc tggaagacta cgtttcccgc atgaaagaag
    15241 ggcaggagaa aatctactac atcactgcag acagctatgc ggcagcgaag agcagcccac
    15301 acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg
    15361 agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag
    15421 ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga
    15481 aagcactgac tccgttcatc gaccgtgtga aagccctgct cggcgagcgc gtgaaagatg
    15541 tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa
    15601 tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat
    15661 acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg
    15721 aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg
    15781 gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat
    15841 gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct gcactttccg
    15901 caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt
    15961 tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg attttcgcaa
    16021 tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca
    16081 tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat
    16141 ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg
    16201 acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt
    16261 tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca
    16321 tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg
    16381 taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga
    16441 aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg
    16501 aagaaaccgt gcgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct
    16561 actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc
    16621 cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaattcgaa agcgcgcacg
    16681 gacagtcccc tcgccccctc ggggagaggg ttagggtgag gggaacaggc ccgcacaagc
    16741 aaacttatca gcaatctcag gccggatatt cattcggcct tttacaaaaa cctcaatctg
    16801 cgctattcct ttttctgatt tgacctctca cagcaattag ttcttcttcc tcacttttcc
    16861 gctacaatta tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt
    16921 atcctgctgg caaacctggg tacgcccgat gcccccacac ctgaagcggt aaaacgctat
    16981 ctgaaacaat ttttaagcga cagacgcgtg gttgatacct cacggttgtt atggtggccg
    17041 ttgctgcgcg gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct gtacgcctcg
    17101 gtctggatgg aagacggctc gccgctgatg gtttacagcc gccagcaaca gcaggcgttg
    17161 gcacaacgtt taccggatac gcccgtagcg ctgggcatga gctacggctc gccatcactg
    17221 gaaagcgccg tcgatgaact cctggcagag catgtagatc atattgtggt gctgccgctt
    17281 tatccgcaat tctcctgttc tacggtcggt gcggtatggg atgaactggc acgcattctg
    17341 gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg attacgccga taaccacgat
    17401 tacattaatg cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat
    17461 ctgctgctgc tctcttatca tggcattccc cagcgttatg cagatgaagg cgatgattac
    17521 ccgcaacgtt gccgcacaac gactcgcgaa ctggcttccg cactggggat ggcaccggaa
    17581 aaagtgatga tgacctttca gtcgcgcttt ggccgggaac cctggctgat gccttatacc
    17641 gacgaaacgc tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg
    17701 ggctttgctg cggattgtct ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc
    17761 ttcctcggtg ccggcgggaa aaaatatgaa tatattccag cgcttaatgc cacgccggaa
    17821 catatcgaaa tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac
    17881 tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca ttcgtgaata atgcaaaaag
    17941 gcatgcagcg tacctgggta gagtttgaac tcacagggct gctgatgcgc cgctaacgtc
    18001 tggtaaagca gacggctgtc atccagcagc ggatcgaact ccgccccggc aataaaacag
    18061 ggcggaactt cgcgagtgag atcattatta aacagacagt aatacggtga ctcgcggtcc
    18121 gcgtcgttgc ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt taagccatcc
    18181 cagacaccgc ccaacagacg acgagtcacg gaatcccgta atccgtaaag cccataccac
    18241 agcaaaacac ccgcaatttt accgcaatcg atctgtttat cacgcaacca caacgcactg
    18301 gcgagcgcca gcatggcacc tgcggaatca ccggcaaagc caatgcggga catattgatt
    18361 tgataatcct ccgcctgctg gtggaaataa caacaagcag ccacaatttc ctctatcgct
    18421 tgcggaaaac gcgcttcagg tgaaaggggg taatcaatac caatcaccgt acattggctg
    18481 tagcttgcca gcaggcgcat gatgcgatcg tgggtatcga gattgccgag aataaaaccg
    18541 cctccatgca aataaaatag cgtcgccggg ctatctggct gcggacaaaa gagacgtgtt
    18601 tccacctgcc catattttgt tggaaccatg taagctctgg ttgccatttc tggagcgccc
    18661 gcattccaga atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt
    18721 gcgggccagg acggtaaatc cggctgaaga gtattcacaa cggtcttcat ttcagcagaa
    18781 ataaggtcca gaacaggtag tttgttttcc ggcttcatac aaaaactcct ttcaaattac
    18841 gtcattgtaa ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc
    18901 tgcgtctcgc gcgctcccgt cagattgtgt taacattcgc cgctcagtta accacccgta
    18961 aaaacaacca tgaaatttcc cggtaaacgt aaatccaaac attacttccc tgtaaatgca
    19021 cgcgatccgc tgcttcagca attccagcca gaaaacgaaa ccagcgccgc ctgggtcgta
    19081 ggtattgacc agacgctggt cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt
    19141 tatggattaa gcgccgggca ttcactggtg attgaggatg atgtagccga agcgctttat
    19201 caggaactaa aacagaaaaa cctgattacc catcagtttg cgggtggcac cattggtaac
    19261 accatgcata actactcggt gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc
    19321 agcaatattg aaattggcag ctatgcctat cgttacctgt gtaacacctc cagccgtacc
    19381 gatcttaact atctacaagg cgtggatggc ccgattggtc gttgctttac gctgattggc
    19441 gagtccgggg aacgtacctt tgctatcagc cctggccaca tgaaccagct gcgggctgaa
    19501 agtattccgg aagatgtgat tgccggagcc tcggctctgg ttctcacctc ttatctggtg
    19561 cgttgcaagc cgggtgaacc catgccggaa gcaaccatga aagccattga gtacgcgaag
    19621 aaatataacg taccggtggt gctgacgctg ggaactaagt ttgtcattgc cgagaatccg
    19681 cagtggtggc agcaattcct caaagaccac gtctctatcc ttgcgatgaa cgaagatgaa
    19741 gccgaagcgt tgaccggaga aagcgatccg ttgttggcat ctgacaaggc gctggactgg
    19801 gtagatctgg tgctgtgcac cgccgggcca atcggcttgt atatggcggg ctttaccgaa
    19861 gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc ggaattcaac
    19921 cagtatgagt ttagccgcgc catgcgccac aaggattgcc agaatccgct gcgtgtatat
    19981 tcgcacattg cgccgtacat gggcgggccg gaaaaaatca tgaacactaa tggagcgggg
    20041 gatggcgcat tggcagcgtt gctgcatgac attaccgcca acagctacca tcgtagcaac
    20101 gtaccaaact ccagcaaaca taaattcacc tggttaactt attcatcgtt agcgcaggtg
    20161 tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc agcattcacc tcgtttaacg
    20221 cgcggcttgc cggagcgtga agacagcctg gaagagtctt actgggatcg ttaagttatc
    20281 gaccagaccg gacgacgatg gcatccgtag gccggataag atgcgctagc atcgcatccg
    20341 gcaataacgt cttgttttac cccgtcacca cctcacctgc cggtggcgtt tccagcaatt
    20401 ccagcatagt acgggcaatt tcacgttcac ccatcactac ctgattcgcg ccacgttcgg
    20461 tgatatacgt cacttcatcg tcataatggg cgcgggcgat aatctcaata tccgggtttt
    20521 tcgcgcgggc agacgccaca atctcacccg cttcataacc gttggggatc gtcaggatca
    20581 gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttcgct gcattgccca
    20641 atacagcgcg gaccccacgc tctcgcagct catcaacacg ggttcgtgac gtctcaatca
    20701 ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct acacgaccgt
    20761 aacccaccaa tagcgcatgg ttgcaaatat ccactgggat ctgtttctct tcttcgattg
    20821 cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc tccagtagtg
    20881 cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc aggttttgtc
    20941 cggcctgcgg cagtaaattc aatgccattc ccagtcccgc caggataaac gcgaactcac
    21001 caatctgcgc caggctggcg gcgatggtta atgccgtacg ttgggagtga ccaaacagtc
    21061 gcaccaggaa aaatgcggct aacgacttac caaacagaat aatcgccagc gtcgccagca
    21121 ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg gagacaaaaa
    21181 acagcaccgc aaacgcgtcg cgcaatggca gcgtatcgtg ggcggcacgg tgactcagtt
    21241 cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca
    21301 gctctaccgc accaaaggca atccctaacg ccagcgccag caccgacagg gtaaacagct
    21361 cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg caccagacgg cgacctacca
    21421 gcatcataat ggcgataaat gcgatcactt tgccgatggt gatccccata tcgactgcaa
    21481 gagtggcaaa gcccacatcg ccctgttcca tcattcctgc cactgcgggc agcaacacca
    21541 gcgtcagaac cattaccagg tcttccacaa tcaaccaacc gatggcgatt tgcccacgct
    21601 gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg
    21661 aaagacataa accgaacacg ataccggtca ttaacgacca gcccagcacg gcagagagcg
    21721 ccatacccag cagcgtcgcc acggctatct gggcgatcgc accgggaatg gcgatggcct
    21781 ttaccgccat caaatccttc agcgaaaagt gcaaaccgac gccgaacatc agcaaaataa
    21841 cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg
    21901 gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt agtttattgg
    21961 ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg ataagcgggg
    22021 tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg gccggggaaa
    22081 ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgttcatga ataattattg
    22141 aattttgcag aaaaatggaa ttagctgcaa aaaaagcacg gatcggaaaa cagaaggcgg
    22201 tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt tgcctttggc
    22261 tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac atccccaata
    22321 gtggcaggaa agcacagatt ttatagacta actcgatact ggtgtgatcg gcgataagcc
    22381 ccagaactgc cgctcccaga cctcccatac caaaagcaaa accgaaaaag agtccagaaa
    22441 ccataccgat acgtcccgga agcagctcct gagcgtaaac cagaatggca gagaatgccg
    22501 aagcgaggat aaatccaata atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg
    22561 gtaaaatcag cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca tatttccgcc
    22621 caattttatc ccctacaggc ccgccgatca ccgtacctgc cgcaacggca aacaggaagg
    22681 caaacagatg aagctgagca ttctggatag ataatccgaa tttttgcatc agataaaagg
    22741 tgtaatagct gctgatgctc gccatataga aatatttcga gaaaatgagg attaacagaa
    22801 tgctgaccgc cagtaccact ttgttgcgcg gcagtggatt gataatcgtc gctttgggtt
    22861 ttcctttatt cattcggtgc tgtgccgagt accaacggct gatttgcgcc aacaccacga
    22921 tcgccagcag tgccgcaagc acaaaccagg caacgttgcc tttgccataa ggcgcgataa
    22981 tcaccgccgc cagcaagggt cccagggaac tgccaaagtt gccgccgacc tgaaagatag
    23041 attgcgccag gccatgccgc ccgccggaag ccatacgggc tacgcgagaa gattccggat
    23101 gaaagaccga tgaaccggta ccgaccagcg ccgccgccag cagaactgcg ccaaaactgc
    23161 ccgccagcgc aagcagcacc agaccactta aggtaaagca catgccaatt ggcaacgacc
    23221 acggcatcgg atatttatcg gtccagtagc cgaccactgg ttgcagtagc gaagaggcga
    23281 gctggaaggt gagggttatc atgccaatct gcataaatgt cagagaaaat tctgactgaa
    23341 gcagcggata aatcgccaga atcagcgatt ggatcatgtc gttcagcaga tgtgagaggc
    23401 tgatagcacc taaaatacca aacgatgttc gggccttggt cgttgacgca gccgcgcccg
    23461 ccacaggctg gggttgttca ctcattgcca taggaaagtc actttttcag ggttgcgatg
    23521 taaagaatga tcttatttgt gattattacc cgactaacat acctgtatgc gtcgcctgaa
    23581 ggaagtctca acgccgaata cagaatttct aatctggatg cagatttatc ttcaccggac
    23641 gcagacttgt ctatgatgtc gcgtcatact atttttcaac acgttgaaat caggtcaggg
    23701 agagaagtat gaaattattg cagcggggcg tggcgttagc gctgttaacc acatttacac
    23761 tggcgagtga aactgctctg gcgtatgagc aggataaaac ctacaaaatt acagttctgc
    23821 ataccaatga tcatcatggg catttttggc gcaatgaata tggcgaatat ggtctggcgg
    23881 cgcaaaaaac gctggtggat ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc
    23941 tgctactttc cggtggcgac attaacactg gcgtgcccga gtctgactta caggatgccg
    24001 aacctgattt tcgcggtatg aatctggtgg gctatgacgc gatggcgatc ggtaatcatg
    24061 aatttgataa tccgctcacc gtattacgcc agcaggaaaa gtgggccaag ttcccgttgc
    24121 tttccgcgaa tatctaccag aaaagtactg gcgagcgcct gtttaaaccg tgggcgctgt
    24181 ttaagcgtca ggatctgaaa attgccgtca ttgggctgac aaccgatgac acagcaaaaa
    24241 ttggtaaccc ggaatacttc actgatgtcg aatttcgtaa gcccgccgat gaagcgaagc
    24301 tggtgattca ggagctgcaa cagacagaaa agccagacat tattatcgcg gcgacccata
    24361 tggggcatta cgataatggt gagcacggct ctaacgcacc gggcgatgtg gagatggcac
    24421 gcgcgctgcc tgccggatcg ctggcgatga tcgtcggtgg tcactcgcaa gatccggtct
    24481 gcatggcggc agaaaacaaa aaacaggtcg attacgtgcc gggtacgcca tgcaagccag
    24541 atcaacaaaa cggcatctgg attgtgcagg cgcatgagtg gggcaaatac gtgggacggg
    24601 ctgattttga gtttcgtaat ggcgaaatga aaatggttaa ctaccagctg attccggtga
    24661 acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctt tacactcctg
    24721 aaatcgctga aaaccagcaa atgatctcgc tgttatcacc gttccagaac aaaggcaaag
    24781 cgcagctgga agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag
    24841 tgcgttttgt acagaccaat atggggcggt tgattctggc agcccaaatg gatcgcactg
    24901 gtgccgactt tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa gcaggcgata
    24961 tcagctataa aaacgtgctg aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca
    25021 tgaccggtaa agaggtgatt gattacctga ccgccgtcgc gcagatgaag ccagattcag
    25081 gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa agacggcaaa ctgaacgacc
    25141 ttaaaatcaa aggcgaaccg gtcgatccgg cgaaaactta ccgtatggcg acattaaact
    25201 tcaatgccac cggcggtgat ggatatccgc gccttgataa caaaccgggc tatgtgaata
    25261 ccggctttat tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg ccgctggatg
    25321 tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt gccggatgtt
    25381 tgcatccggc acaatgctta atcgcggcgg gcgatatcag caaatttggc atcgaggatc
    25441 tttgccagat cgcctgccgc cagttcgata tccagtccgc gcttgccgcc ggaaacataa
    25501 atagtggcaa attcttgtgc gggggcgtcg ataatcgttg gcagacgttt tttctgcccc
    25561 agtgggctaa tccccccaac caggtatccc gtcgaacgct gcgcgaccat cggatcggcc
    25621 atctcaactt tcttggcacc cagcgctttt gctacttttt taagatccag ttgacctgcg
    25681 accggcgtaa cggccacggc aaggtgtttc atatcaccgt tcactgccac cagcagcgtt
    25741 ttgtagacct gatccggatt caaacctaat tttttgacga cttcatcgcc aaaattggtt
    25801 tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt ttcgagtaat
    25861 ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag caagcatacg
    25921 cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag gatggtgaga
    25981 ttgagcgaca atcgagttac accgtcgctt aaagtgacgg cataataata aaaaaatgaa
    26041 attcctcttt gacgggccaa tagcgatatt ggccattttt ttagcgcaac atttgcggca
    26101 aattcccttc tccatacagg tgtagtgcac cgaccgcgac cacatatcgc cccggcggca
    26161 tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg catcagcaca tcgtacagcg
    26221 actgactgaa cgtattgggc agcgttatat cattattttg cggaggtgca ttcagccacc
    26281 agctcatcat ttgttgcagc aaccgtgcgt tggtatgcca gtgggtcagc gtatcgtcca
    26341 gcagcgccag tcctttgtca gggagctgga gcaacatggt aatctggttt tcagcccctt
    26401 ccagttcaat cacgggttta tgttgttgct tcgccgcctg caataactgg taatcgatac
    26461 cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt
    26521 gccacagcgg ttgagtagaa aagagcgacg gagaaatgcc catctcctgg ctaatgtgct
    26581 gcagattttg gagttgctcc tcgttaatgc gctcttccag cgcctcgcaa gtaggcaaat
    26641 tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt
    26701 tgagcttttt gagcaaacgg gtgggcaggg gagccatatc gtggctaccc atatgaatac
    26761 tgccaatcag atgaaaatgg cgattgccgg gaagggtgat atcgatggca ggccaggtgt
    26821 aatgattacc gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga tccatacgac
    26881 ctccctttgt gaaatatcat gctagcgcgc ggtgagggat ggcgcaaatg gagttgggga
    26941 cttgtttaat gtgtttgtat gattcagtat gttcttgcat cgctattcac agggaagcaa
    27001 cagttaaaaa ccatgaaaca ggcaacaaga aaaccgacga cacccggaga tattcttctc
    27061 tatgaatatc tggaaccgct cgatttgaaa atcaatgagt tagcagagtt gctgcatgtt
    27121 catcgtaata gcgtcagtgc actgatcaat aacaatcgta aactcaccac tgagatggca
    27181 ttccgtctgg cgaaagtttt tgataccaca gtcgatttta ggctaaacct ccaggcggca
    27241 gttgatcttt gggaagttga aaacaacatg cgcacccagg aagaattggg acggattgaa
    27301 acagtggctg aatatttggc acgccgtgaa gagcgtgcaa aaaaggtcgc gtaaggccaa
    27361 aacatcgctt catctcgtag gccggataag gcgtttacgc cgcatccggc agtcatgcgt
    27421 cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta ttccttcggt
    27481 ttaaaccgca gcaaccggtt ggcgttactc actacggtaa tcgacgagag cgccattgcc
    27541 gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg gccacaaaat accggcggcg
    27601 accggaatac cgatgctgtt gtagataaag gcaccgagca ggttctgctt catgttgcgc
    27661 agcgttgcgc gggaaatcgc gagcgcatcc gcaacgccca tcaggctatg gcgcatcagg
    27721 gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccatcgcaat gccgacatcc
    27781 gcctgggcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac ctgacgtcct
    27841 tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcactcc ggcaatcact
    27901 tcatcaattc cagcttcttt ggcgatcgca ttggcggtgg ttgggttatc cccggtcaac
    27961 atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct atcactacgc
    28021 aacggatcgc gtacagccag cagggctact gctttcccgt caatcgccag cagcacaggc
    28081 gttgctcctt gcgatgcctg agcagtaatc tccgcttcga tagctttggt accaacctgt
    28141 tgctcattta acagcgcctg attgcccagc aataacgcat gaccttcagc ttcaccgctc
    28201 acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gcagctgcat atcacctgct
    28261 ttatcgagga tcgcccgtgc cagcggatgg ctggaacctt gctccagtgc cgccgccaga
    28321 cgcaatgcct gcgcttcatc aacatcagca aacgttttca ctgcgacaac ctgcggcttc
    28381 ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact ggcgcgttgc
    28441 agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac gccggaaata
    28501 atcgacatcg gcgtcgccag ccccagcgca cacggacagg cgataatcag taccgtggtg
    28561 gcaatcacca gggtataaac aatttgcggt gccggaccaa agaagtacca gattgccgca
    28621 ctgaccagcg caatcaccac caccacaggt acaaatacgg ctgagatttt atccgccagc
    28681 tgaccgattt ctggcttgct gctctgggcc tggcgcacca tgcgaatgat ccgtgacagc
    28741 gtggtatggc tgccaaccgc actggcacga aacagcacgc taccgtcctg taccactgtc
    28801 ccggcatgga cgctttcgcc ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc
    28861 gcttcatcca gccatgcttc gccctgggta atctcgccat ctaccggcac gcgatcgccg
    28921 gtcgtcaggc gcagcaacat acctggctgc acttccgcca gaggcacgct tttttcacct
    28981 tcgtcagtaa ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc cagcgcctta
    29041 gaagaacgct gacgtgcgcg tgcttccagc atatggccga gattgatcag accgataatc
    29101 atcgcgctgg cttcgtaata aagatgtcgc gcttccatcg ggaaccactg cggccacagg
    29161 ttgacgctca tcgaatagag ccacgccacg ccagtaccca gcgccaccag cgtatccatc
    29221 gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc gccggcgaaa
    29281 accatcaccg ccagggttat caggccgata accaaccaca ggctgcggtt gtcagcggtg
    29341 accatcatgt tatcgccaat catcccccag accatcaccg ggatacccac cgccagtgcg
    29401 acaattgcct gccagcggaa gcgcttcatc gtagcaacgg cggtttcctg ctggcgctca
    29461 cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc
    29521 actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc caggtttacc
    29581 cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt gtacgcgggt gacacagctg
    29641 gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc
    29701 ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc cgccagcggt
    29761 ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt ttcgatcagc
    29821 tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga tagacacatc cgcttgttcc
    29881 acatccgggc gctgttcgag actttctttc acgcgtttaa cgcagtgacc gcaggacagg
    29941 ccgtccaggg tcaggtcgat aatttgtgac ataaaacact cctttaagac agttttgact
    30001 ggctgtgata aaggttaaac cttccagcaa ggggaaggtc aagaaattaa taaaccaggc
    30061 gagtaaaagt ccgtaaagat taaaaaattg gctcaatttg catcaggatt agacatttat
    30121 ctctttgttt tcctgtagtt aagttgcggg tgctaagtta aatcaggatg cctcaaaatc
    30181 ggcaccgggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa aagatgttag
    30241 atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac tcacttaacg
    30301 gcggtcaaaa tgccgactac attccctttc tggcgaatgt gcctggtcaa ctggcggcag
    30361 tggctatcgt gacctgcgat ggcaaggtct atagtgcggg tgacagtgat taccgctttg
    30421 cgctggaatc catctcgaaa gtctgtacgt tagcccttgc gttagaggat gtcggcccgc
    30481 aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac tcagttatcg
    30541 ccttagagtt gcatggcggc aaaccgcttt cgccactggt aaatgctggc gccattgcca
    30601 ccaccagcct gattaacgct gaaaatgttg aacaacgctg gcagcgaatt ttacatatcc
    30661 aacagcaact ggctggtgag caggtagcgc tctctgacga agtcaaccag tcggaacaaa
    30721 caaccaactt ccataaccgg gccattgcct ggctgctgta ctccgccgga tatctctatt
    30781 gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctccacgctc ctcaatactg
    30841 ttgaactggc aacacttggc gcgacgctgg cggcgggggg agtgaatccg ctgacgcata
    30901 aacgcgttct tcaggccgat aacgtgccgt acattctggc cgaaatgatg atggaagggc
    30961 tgtatggtcg ctccggtgag tgggcatatc gcgttggttt accgggcaaa agcggtgtag
    31021 gtggcggtat tctggcggtc gtccctggtg tgatgggaat tgccgcgttc tcaccaccgc
    31081 tggacgaaga aggcaacagt gttcgcggtc aaaaaatggt ggcatcggtc gctaagcaac
    31141 tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata acggtaacaa
    31201 acccctcggt ctatggaatg tcgtttccat cggtattggg gcaatggtgg gggcggggat
    31261 cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc tcgacctggg tcgcctttgc
    31321 ttttggcggt attgtggcga tgttttccgg ttatgcctat gcgcgtctgg gggcgagcta
    31381 tcccagcaat ggcggcatta tcgacttctt tcgtcgcgga ttaggcaacg gcgtcttttc
    31441 gctggcgctc tcgttactgt acctgttgac gctggcggtg agcatcgcca tggtcgcccg
    31501 tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg agcaccttat
    31561 tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct taagcaacca
    31621 tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc tgttattgct
    31681 gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg cgccccccag
    31741 ctccggtgcg ttcttctcct gtatcgggat aaccttcctt gcctatgcgg gctttggcat
    31801 gatggcgaac gcggcggata aagtgaaaga tccgcaggtc attatgccgc gggcgtttct
    31861 ggtggcgatt ggcgttacca cgttgcttta tatctcgctg gcactggttt tgcttagcga
    31921 tgtatcggca ttagagttag aaaaatatgc cgataccgcc gtagcgcagg ctgcttctcc
    31981 gctgctcgga catgtgggtt atgtgatcgt cgtcatcggc gctttactgg cgacggcttc
    32041 agccattaac gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg gcagcgaacg
    32101 cgaactgccg aagctaatga ataaatccct gtggcggcag agtacctggg gcaacatcat
    32161 tgtcgtggtg ttgattatgc tgatgacggc ggcgctaaat ttaggctcac tcgccagtgt
    32221 tgccagcgcc acctttttga tttgctacct ggcggtgttt gtggtggcga tccgcctgcg
    32281 tcatgatatt cacgcctcgt tgccgattct tatcgttggt acgttggtga tgttgttggt
    32341 gatcgttggc tttatctaca gtctgtggtc ccagggtagc cgtgcgttga tatggattat
    32401 tggctcactc ttactcagcc ttattgtggc aatggtcatg aagcgcaata aaaccgtata
    32461 acatctctct gtgcgcagta cttcctgtat tattgtggtg gcggtcgata ttcgcactgg
    32521 caaaaaaacg tgcttgaata tctgttgaaa ccctttaaca aagcacagga ggcgttgcgc
    32581 gaacgatgaa catcagcgat gtagcaaaaa ttaccggcct gaccagcaaa gccattcgct
    32641 tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt tatcgcacct
    32701 acacgcagca gcatctcaac gaactgacct tactgcgcca ggcacggcag gtgggcttta
    32761 acctggaaga gagcggcgag ctggtgaatc tgtttaacga cccgcagcgg cacagcgccg
    32821 acgtcaaacg gcgcacgctg gagaaggtgg cggagatcga acgacacatt gaggagctgc
    32881 aatccatgcg cgaccagctg ctggcactgg cgaatgcctg ccctggcgat gacagcgccg
    32941 actgcccgat tatcgaaaat ctctccggct gctgtcatca tcgggcaggg tgattaagac
    33001 gagacggctc ggatatgtag ggttatccct tctatcgcaa tgacttcaac atgcgtacct
    33061 gcaccgagat cctcgctggc gctgacaggc catgaactgt caccgacgcg catatgaccg
    33121 cgcccgttga ccagcggaga ttccagcaca aaacgtcggc caatcagctg ctgcccgcgc
    33181 tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc gccgcgacaa ccatttccac
    33241 cacagccagg cggcgagcag cgtcaggatg gcaaacatta ccccttgcca ctcccaaccc
    33301 agcggcacca gccagaccac caggccggta atcaccgctg ccacgccgct ccacaacaaa
    33361 taaccatttc cgcccagcat ctcggctgcc agcagcaaac cgccgagact gagccagaaa
    33421 atatgtggat gaacgaccat taactccatc atggctgagt ccgtttgttg gcgctgtctt
    33481 tcaccagctc ggcaatcccg gcaatcgacc ccatcaggct gctggcctct aatggcatca
    33541 tcactacttt gctgttactg gaggaaccga tctgctgtaa cgcttcggtg tatttctgcg
    33601 ctacgaagta gttcaccgcc tgaatatcac cggaggcgat ggcttcagac accattttgg
    33661 tggcgcgggc ttctgcttca gcggaacgtt cacgcgcttc agcctgtaaa aacgccgact
    33721 gacgttcgcc ttccgctttc aggatttgcg actgtttttc accttcggct ttgaggattt
    33781 ccgcctgacg gatcccttcc gcttcaagaa tgtaagcgcg tttggtacgt tccgctttca
    33841 tctgcgcgtt cattgaagag ataagctctg ccggtgggcg cacgtcgcga atttcaatac
    33901 gggtgacttt aatcccccac gggttggtgg cttcatcgac aatacgcagc aggcgtgagt
    33961 tgatgctgtc gcgctgagag agcatttcgt caagttccat tgaacccaac acggtacgga
    34021 tgttagtcat ggtcaggttg atgatcgcca gctccagatt gctgacttca taagccgcgc
    34081 gcggcgcgtc aatcacctga ataaaacaga ctgcgtcgat ggtaacgttg gcgttatctt
    34141 tcgagataac ttcctgggaa gggatatcga gcacttgctc catcatattg atcttgcgac
    34201 caatgcgatc cataaacggc accaccagac tgagccccgg ctgtaacgtt ttggtatagc
    34261 gaccaaaacg ttctaccgtc cattgatagc cctgcggcac gattttgacg cccgcgccga
    34321 caatgaccag cgcgacaaaa atgagaatcg ggataaagat aagcatcgga aaaacctcct
    34381 gttgtaccgt ccataatcag caaaattgct gcttgattaa acaaattata cctgattact
    34441 gaaagagagt tcccccttat tcctgcgaag gataaactgt ttttagtaaa aatcagaaaa
    34501 agggaacagt gatgcaggaa aatagtcctt tgcttcagct acaaaacgta ggatatctgg
    34561 cgggtgatgc gaagattctt aataacatca atttttcgct gcgtgctggc gaatttaagt
    34621 taattaccgg tccttccggt tgtggcaaaa gtacgctgct aaaaatagtt gcttcattga
    34681 tcagcccaac cagcggaacg ttactgtttg aaggtgagga tgtcagcaca ctaaagccag
    34741 aaatctaccg ccagcaagtc tcttactgcg cccagacgcc gacgctgttt ggcgatacgg
    34801 tatacgataa tctgatcttt ccctggcaga tccgtaacca gcagcctgac ccggccattt
    34861 ttctcgattt tctcgaacgc ttcgctttgc cggacagcat tttgacgaaa aatatcgccg
    34921 agctatctgg tggtgaaaaa caacgcatct cactgattcg taacctgcaa tttatgccga
    34981 aggttttatt gctggatgaa atcaccagtg cgctggatga aagtaataaa cataacgtta
    35041 atgagatgat ccatcgttat gtgcgcgagc aaaatattgc cgtgctgtgg gtgacacacg
    35101 ataaagacga aattaatcat gcggataaag tgattacact gcaaccgcat gccggagaaa
    35161 tgcaggaagc acgctatgaa ctcgcataat attactaacg aatcattggc actggcatta
    35221 atgctggtgg tggtggcaat cttaattagc cataaagaaa aactggcgct ggagaaagat
    35281 attttctgga gcgtcgggcg agcgataatt cagctgatta ttgtcggcta tgtgctgaag
    35341 tatattttca gcgtggatga tgccagcctg acattattga tggtgttatt tatctgcttt
    35401 aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg ctaaagcttt tatctcatcg
    35461 tttattgcca ttacggtcgg ggcgggaatt accctggcgg tgctgattct ctctgggtcg
    35521 attgaattta tcccgatgca ggtgatccct atcgccggga tgattgccgg taacgccatg
    35581 gtagcggtgg ggttgtgtta caacaattta gggcaacggg tcattagcga acagcaacag
    35641 attcaggaga aactgagtct tggtgcgacg ccgaagcagg cttcagcgat attgattcgc
    35701 gacagtattc gcgcggcttt aattccgacg gtcgattcag caaaaacggt tggcttagtg
    35761 agtttaccag gaatgatgtc cgggctgata tttgccggga ttgatccggt gaaggcgatt
    35821 aaatatcaga ttatggtgac ctttatgctg ctctcaaccg ccagcttgtc gaccattatt
    35881 gcctgctatt taacctatcg taagttttat aattcgcgcc accagttggt ggtgacgcaa
    35941 ttgaagaaga aatgatgcga tgccggatgc gcagcatcgc atccggcgtt gtggttcatg
    36001 tgccgatcaa cggaatatca atacaacaat gcatacagct ggcggcgata cttcgacgcc
    36061 agtgcatcac ccgtacccag cgcagcgagg atctcctgga acgttttacg cgtctgaccg
    36121 tctgcggcgg tgagatcttt acgcagatgc ccgaacagca actccagcgc ctcttcattg
    36181 cgcccaacct ggtgcagttg cagagccagt tgcgtcgcca gtgcggcatc ttctggattc
    36241 tccgccacct gctgttgcaa ctgttgaatt tccggcgtat cagccgcctg cttcagcagt
    36301 tcgatttgcg ccaccagccc ctggtagcgg gtgtcctgat cctgcaacgg aatggttttc
    36361 agcaccgctt ccgcatcttc agaacggttc agcgcaatca gcgtttctgc cagcagcagg
    36421 ccgatctccc cgttctgatt cgacaactgc caggcgtctt tcagcaatgg cagggcatcg
    36481 gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg ctttcagctc ttcttcgcgc
    36541 ggcagcactt tatccagcag ggcgcggatc gcctcttccg gttgcggccc ctggaagcca
    36601 tctaccggtt gcccgttctg gaacagatac acggtcggaa tcgcgcgcag accaaactgg
    36661 gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca gaataaattg cccgttgtac
    36721 tgcgccgcaa ggctttccag aattggggtt aactgcaaac agtgctggct acgttcagac
    36781 caaaaataga acagcaccgg agtggtcatc gactgttcaa gaacctgttg caggttagat
    36841 tcgttaatgt tgacaatatt ttctacggac atggagtcgc tctctgttgt cgatattttc
    36901 tttgacatgg gggcttaagc gcgcgcttca actcacccct gcaatatttt gtccatcacg
    36961 cgccccggca gcaggcgctt aagcaccatt actgcccagg tcaccagcgt taccggatag
    37021 cgcatcttcg gcttctcgct aataaaagca tggcgcactt tgtccaccac cgcttccggt
    37081 cccaacgtaa agcgggcggc gatgccggga ttttcgactg gtttatcact ttgcgtctgg
    37141 ttgacgttgt cggtgaagcg ggtacgaata ggaccgggtt cgatcaggct aactttaatt
    37201 ccgctgtggc gcagctccat gcgcagtgcg tctgaccacg cctccagcgc atatttactg
    37261 gccgcgtaag cgccgcgacc cggcgtggag attaatccca tcaccgatga tgtcatcaca
    37321 atacgccctt caccgtgcgg taacatcgcg ggtaacaggc gtatggtgag ctggtgtgcg
    37381 ccgaaaaagt tggcggaaaa ctgctgttcc atctgcgcgc ggctgatggt ggaaaggggg
    37441 ccatacatgc cgaatccggc attgttaaag atcccataca gacaattatc ggtcagggcg
    37501 atcacttcgt cggctgcgcg atcaacactt tctggcgaat ccagatcgat caacacgccg
    37561 gtaaatccca tgctgttcat gcgctcaaca tcatccggtt tccggcaacc tgccagcaca
    37621 tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc caattccact ggaacatccg
    37681 gtaattaaga ccgatttttg cataacttta cctgtcagga tctccgttgc tttatgagtc
    37741 atgatttact aaaggctgca actgcttcgc catccagtcg gcaataaacg gctgggcgtc
    37801 gcggttggga tgaataccgt catcctgcat ccattgtggc ttgaggtaga cctcttccat
    37861 aaaaaagggc agcagcggaa catcaaactc tttggcgagt ttggggtaaa tggcgctaaa
    37921 ggcttcatta taacggcgac catagtttgc aggcagacgt atttgcatta acaatggttc
    37981 agcgttggcg gctttgacat cctgcaaaat ctggcgcagc gtttgctcgg tttgctgtgg
    38041 ctgaaaacca cgcaaaccgt cattgccgcc cagttcaacc agcacccaac gcggctgatg
    38101 ctgtttcagc agagccggaa ggcgcgccag tccttgttgc gaggtgtcgc cgctgatgct
    38161 ggcattaact accgacgttt tactctgcca cttatcattc aacaaggcag gccaggccgc
    38221 gctggcagac attcgatacc cggcgctcag gctatcaccc agaatcaata acgtgtccgc
    38281 tgcggcggca cggaaggtta acaggaccag gaacaggaag ggcaaatgcc agcggaaaac
    38341 attgttgaag ttcatcatct taagaagtcc gtcggtcagg gggagcatga actctccatc
    38401 ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca ttgcactggt gggcgagtcg
    38461 ggatcgggta agtcaacctt gctggcgatc ctcgccgggc tggatgacgg cagcagtggc
    38521 gaagtgagtc tggtgggaca accgctacat aatatggacg aagaagcgcg ggcaaagttg
    38581 cgcgcgaagc acgtaggctt tgtttttcag tcatttatgt taattcctac ccttaacgcc
    38641 ctggaaaacg tcgagcttcc ggcgctgctg cgcggtgaga gtagcgcgga aagtcgtaac
    38701 ggggcgaaag cgttgctcga acagttaggg ctgggtaaac gtcttgatca tcttccgaca
    38761 cagctttccg gcggcgaaca gcaacgagtg gcgctggcgc gagcctttaa tggtcgacct
    38821 gatgtgctgt ttgccgacga acccaccggc aaccttgacc gccagacggg cgataaaatt
    38881 gccgacttgc tgttttccct caaccgtgag catggcacca cgttgattat ggtgacccac
    38941 gacctgcaac tggcggcacg ctgcgaccgc tgcttacggc tggtgaacgg gcagttgcag
    39001 gaggaagcat gattgcacgt tggttctggc gcgaatggcg ctcgccgtcg ctattaattg
    39061 tctggctggc gctaagcctg gcggtggcct gcgtgctggc gctgggcaat atcagcgatc
    39121 gcatggagaa gggcttaagc cagcaaagcc gggagtttat ggcgggcgat cgggcgttgc
    39181 gcagttcgcg cgaagtgccg caagcgtggc tggaggaagc gcaaaagcgc ggcctgaaag
    39241 tcggcaagca gctgactttc gccaccatga catttgcagg cgacacaccg cagctggcga
    39301 acgtcaaagc ggtggatgat atctatccga tgtatggcga tctgcaaacc aatccccctg
    39361 gcctgaaacc gcaggcgggc agcgtattgc tggccccacg cctgatggca ctacttaacc
    39421 tgaaaacggg cgacaccatt gacgtgggcg atgccacctt gcggattgcc ggagaagtga
    39481 ttcaggaacc ggattccggt tttaacccct tccagatggc tccgcgtctg atgatgaatc
    39541 tggcggatgt cgataaaacc ggagccgtgc agccggggag tcgggtcacc tggcgttata
    39601 aattcggcgg caacgagaac cagctcgacg gctatgagaa atggttgtta ccccagctta
    39661 aacccgaaca acgctggtac ggtctggaac aggacgaagg cgcgctgggg cgatcgatgg
    39721 aacgctcgca acagttcctg ctgctttcgg cgcttctgac cttgctgctg gcagtggcag
    39781 cggtggcggt agcgatgaat cattactgtc gcagtcgcta cgatctggtg gcgatcctca
    39841 aaacgctggg ggcagggcga gcgcaactgc gtaagctaat cgtcggtcag tggttgatgg
    39901 tgctgacgct ttcagccgtt accggtggga ccataggcct gttgttcgaa aacgtgttga
    39961 tggtgctgct caagcccgtt ctgcctgctg cactaccgcc agccagcctc tggccgtggc
    40021 tgtgggcgct tggcaccatg acggtcatct cgctgctggt ggggctacga ccataccgct
    40081 tgttgctggc aacgcagcct ttacgcgtat tacgtaatga tgtggtagcg aacgtctggc
    40141 cgctgaagtt ttatctgccg attgtcagtg tggtggttgt gctgctgctc gccggattaa
    40201 tgggtggcag catgctgctt tgggcggtgc tggcgggcgc ggtagtactg gctttgctgt
    40261 gcggtgtgct gggctggatg cagctgaatg tacttcgccg catgacgctg aaatcgctac
    40321 ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg gtcaacgtta agccagcttt
    40381 cggcattttc gctctccttt atgctgctgg cgctgttgct ggtattgcgt ggcgatctgc
    40441 tcgaccgctg gcagcagcag ctaccgccag aaagcccgaa ctacttttta attaacatcg
    40501 ccacagaaca ggtagcaccg ctaaaagcgt tcctcgcgga acatcatata gtcccggaat
    40561 cattttatcc ggtagtgcgg gcgcggctga cggcgattaa cgataagccg acagaaggta
    40621 atgaagatga ggcgcttaat cgcgaactca atcttacctg gcaaaatacg cggcccgatc
    40681 ataatccgat tgtcgccggt aactggccgc caaaagccga tgaagtgtcg atggaagagg
    40741 ggctggcgaa acgcttaaac gttgccctcg gtgataccgt gacttttatg ggcgataccc
    40801 aggagttccg cgctaaagtg accagcctgc gcaaagtgga ctgggaaagt ctgcggccta
    40861 atttctattt tattttccct gaaggggcat tagacgggca accgcagagc tggcttacca
    40921 gtttccgctg ggagaatggc aacggcatgt tgacacaact caaccgccag ttcccgacca
    40981 ttagcctgtt agatattggc gcgattttaa aacaggtcgg tcaggtgctg gagcaggtaa
    41041 gtcgggcgct ggaggtgatg gtggtgctgg tcaccgcctg cggtatgttg ctgttgctgg
    41101 cacaggtgca ggtgggaatg cgccagcgtc atcaggagct ggtggtatgg cgcacactcg
    41161 gtgcggggaa aaaactgctg cgcacgacat tgtggtgtga gttcgccatg cttgggtttg
    41221 tttccggcct ggtggccgca attggtgcgg aaacggcact ggcagtgttg caggcgaaag
    41281 tgtttgattt cccgtgggag ccagactggc gattgtggat tgtgctgccg tgcagcggtg
    41341 cgctgctgct gtcgcttttc ggcggctggc tgggcgcgag actggttaag ggtaaggcgc
    41401 tgttcaggca gtttgcgggg tgatgacagg cgttagattg cctgatgcgc tacgcttatc
    41461 tggtctacga agatcgtgca atatattgat tttgcaagat tgtgtaggcc ggataaggcg
    41521 tttacgccgc atccggcatg aacagtgtgc attttggcaa taaatatgaa accaactgtc
    41581 atgtcggttt ttttaaaggc cgaaggtggt aaaccttcgg ccttagctac gtttcctcag
    41641 ttattcaata aactgacgga tcagttccag cagcgcgatt agcgccttaa gaacgatgag
    41701 aactttatcg agaaaagttt tcatccgttg ctcccttaag ggagaaagac aatcacagct
    41761 tacccttacg ttgcctgtaa ctatgatttt cttcatgtgc tagatgagga ctgcctctct
    41821 ccaaattaga gcaccagaat cagcaccacc tgtatcctga ccaggactta aaagtacttt
    41881 tccgcactgc ggtaatccct tgaggcagta tcactactag cgtcgggatt gtatcgccaa
    41941 catagctgtt tgaatatgta ggcatcctga cttataattc gcttgctaga tgaggactgt
    42001 ttcatttctg cccggaacca ccaagtcttg ggcggaaaaa ataaaaaaac cgactgtcat
    42061 gtcggttttt ttatatctgt attctggtgc aaccattctg ttctcacgac aatcgccgtt
    42121 tcagaatacc gcccatcact gcgccttaac ccattccgcc acttccgccc actcaccgcg
    42181 aaagacaact ttttccgctt ttttctcaag ctgatagcga tacattgggt cgtaatattc
    42241 tttaagtaac ggcaccagcc aggccagatg accgtcggtg ctgccggtgg tgagttgcgt
    42301 tgtcagcgcc gtatccagct gggcagcgag ttcgttatag cgctgtaacc ccagccgacg
    42361 cttaatcgcc gaaagtccgt gatgcaggta ttcgcaatat tcctgccagc cctgttcgtc
    42421 gccataagcg tgggtaaaat catgatgcat acgcaagaaa tactcttcgt tcaggcgttc
    42481 aagacggatc tcaaacggat cttctaccac cgcaatcgcc gcctgagtca ttcgctcgcg
    42541 cagacattcc ggcaggtgat tcgaaccgat catccggctt tcgtcttcca gcacccacag
    42601 gcgcaaatcc tgacgggcgt cggttttcag catttcggca gccagcaggt tttcaaaact
    42661 cgcctggcta agttgtggtt gtaacgtgcg accaaacgcc gaaccgcgat ggcgcgccag
    42721 cccttccaga tcaacaccgt tcggctgttg ctgcactaac agcgttttac cgctgccagt
    42781 acaaccgcca atcagcacta tcggtttttg tgccagttca atagtcgcct gaatcgcggt
    42841 ctggcgcagt gccttataac cgccttccac cagcggataa tcaatccccg ctgcatacaa
    42901 ccagctttgc acaatatgtg agcgctgacc gccacgggcg cagcagagaa taccttgcgg
    42961 attttgcagg cacgctgccc gccaggcgtc catgcgctgc tgacgaattt cacccgccac
    43021 cagtttatgc cccagcgcca gcgctgcgtc tgagccttgc tgtttatagc aggtgccaac
    43081 ggcggcgcgt tcatcgttat tcattaacgg cagattgata gcggcgggca ttgcgccttg
    43141 ctcaaactcg ataggggcgc gaacatcaat aatgggcgta tcagcaatca gcagggcacg
    43201 atagtcctgt tccgtgtgtc tctcttgcat agttaaaagt gaacctcaaa tcagcttgcg
    43261 cgctatttta cgcgccaacg cgcaaggaaa cttgattttt aactgcgtgg gttgccgaaa
    43321 atttctaaaa atccgctgat ttccggcctg cgctgggtaa acaaggtcac aatatcttct
    43381 accgctttgc cgctgccaaa tttgcgccat gccagactca atggcgaagg agggcgcatc
    43441 gttgggatta cccggctaac cagttgttga ttatcgatca ttgactggca aagcgatttt
    43501 ggcaaaaaac caatgccaac gcccgcaaga tgggcggcga ttttcgtttc catatcagga
    43561 acaataatct ctttttgccc tggcaatcgc caggcgacgc gtttggttaa ggtgcgggcg
    43621 ctgtcttcaa tattgaccgc cggaaagcgc cgcaactgcg cttctgttag cggctcttca
    43681 acgttcgcca gcggatgatc cgccgccatg acaaagcgcc attgcaccga tcctaaggga
    43741 tcaagactaa aggtatttgc cagcgcctcg gttcccgtga cgccgatagc cagtgaaaaa
    43801 ccttcgtaca atagcgaatc ccagacgccc atatagattt gtcgggagat gtgaaactgg
    43861 gtaaaggggt aacgttcatt cagccacgcc agcaactggg cgacggcctg ggggttgtag
    43921 agcaggttgt tgatgacaat attcacctgg cgttccacgc catcattcac ctgttgcagc
    43981 tcgcttggca tactttccag ccagctcagc cagtctctgg cctgggaaag tagatgctcg
    44041 ccagccgctg tcaacgtcac gctacgagtc gtacggaaaa acagcgctac tccggtattc
    44101 tcttccagaa gtttaatgcg ataactgatc gtcgccgtgg ttttacataa tcgttctgcc
    44161 gcttttgaaa aacttcctgt ttcagcaacc gcaatgaaag tccgcaaggt ttctggatcg
    44221 aacatcttca ggtatcccct tttaaatccg caagttgcgt gattttctta tcctctgatt
    44281 tatcagtatt tttacatgat aaccctgttc aatttgtgga ctaaatctag ttttggaaaa
    44341 atattccaac ttttgtattg atgttgttct cttaaggttt tagattgcct gttattgaaa
    44401 ccaagctgac cggtcggcga tggttgaacg gaattatgtt acaaggacaa aaagatgaaa
    44461 cttcaggtat taccgttaag tcaggaagcc tttagtgctt atggcgacgt aatcgaaact
    44521 cagcaacggg attttttcca tattaacaat ggcctggtgg agcgttacca cgatttggcg
    44581 ctggttgaga ttcttgagca agaccgtacg cttatcagca ttaaccgcgc gcaaccggcg
    44641 aatctgccgc tgaccattca cgaactcgaa cgtcatccgc tgggcactca ggcctttatc
    44701 ccgatgaaag gtgaagtgtt tgtggtggtc gtggcgttag gcgacgacaa accagacctg
    44761 tcaacgctgc gggcgtttat caccaacggc gaacagggag tgaattacca tcgtaacgtc
    44821 tggcatcacc cacttttcgc ctggcagcgc gtcaccgatt ttctgaccat cgatcgcggc
    44881 ggcagtgaca actgtgatgt tgaaagtatt cctgaacagg aactctgttt tgcgtgacgc
    44941 ctgcaaccga cttgcataag ataaactaat tgttcattgt ttatgctcac ttgtaggtcg
    45001 gagttaacgt aggtatgacg gaagttagac ggcgcggcag gccaggacag gcggagcctg
    45061 tggcacagaa gggcgcacag gcgttagagc ggggaattgc gattctgcaa tatctggaaa
    45121 aaagtggggg aagttcgtcg gttagcgata tttctctcaa tctggatttg ccgctctcca
    45181 cgacctttcg cttgctgaag gttttacaag cagcggattt tgtctatcag gacagtcaat
    45241 taggctggtg gcatatagga ttaggtgtct ttaacgtcgg tgcagcgtac atccataacc
    45301 gcgatgtcct ctccgtcgcc gggccgttta tgcgccgcct gatgttactt tccggcgaaa
    45361 cggtcaatgt cgcgatccgt aacggcaatg aagcagtatt aattggtcag ttagagtgta
    45421 aatcgatggt caggatgtgt gcgccactgg gcagtcgtct gccgctgcat gcttccggtg
    45481 cgggcaaagc gctgctttat ccgctggcgg aagaggagtt gatgagcatc attctgcaaa
    45541 ccggtttgca gcagtttacg ccaactacgc ttgtggatat gcccaccttg ctgaaggacc
    45601 tggaacaagc gcgtgaactg ggctataccg tagataaaga agagcatgtt gtaggtctga
    45661 attgcatagc ttcagcaatt tacgatgatg tcggtagtgt tgttgccgct atctccatct
    45721 ccgggccttc atcaagactg acagaagatc gttttgtcag tcagggtgag ctggtcagag
    45781 acaccgcccg cgatatcagc acggcgttgg gactgaaagc acatccataa tgtctgtcgc
    45841 atcccgctct gcggagcggg tttttttgac aaaatttgaa agttggaaaa attttccaat
    45901 aaatagaggt aggaataaaa tggcaaaaat gagagccgtt gacgcggcaa tgtatgtgct
    45961 ggagaaagaa ggtatcacca ccgccttcgg tgttccggga gctgcaatca atccgttcta
    46021 ctcagcgatg cgtaagcacg gcggtattcg tcacattctg gcgcgtcatg tggaaggtgc
    46081 ttcgcacatg gcggaaggtt atacccgcgc aacggcagga aatatcggcg tatgtctggg
    46141 gacttccggt cctgcgggca cggacatgat caccgcgctc tattccgctt ctgctgattc
    46201 cattcctatt ctgtgcatta ccggccaggc accgcgcgcc cgtctgcata aagaagattt
    46261 tcaggccgta gatattgaag caattgctaa accggtcagc aaaatggcgg ttacagttcg
    46321 tgaagcggcg ctggtgcctc gcgtgctgca acaggcattt cacctgatgc gttctggtcg
    46381 tccgggtccg gtactggtgg atttaccgtt cgacgttcag gttgcggaaa tcgagtttga
    46441 tcctgacatg tacgaaccgc tgccggtcta caaacctgct gccagccgta tgcagatcga
    46501 aaaagctgta gaaatgttaa tccaggccga acgtccggtg attgttgccg ggggcggggt
    46561 aattaatgct gacgcagctg cactgttaca acagtttgct gaactgacca gcgttccggt
    46621 gatcccaacg ctgatgggct ggggctgtat cccggacgat catgaactga tggccgggat
    46681 ggttggtctg caaaccgcgc atcgttacgg taacgcaacg ttgctggcgt ccgacatggt
    46741 gtttggtatc ggtaaccgtt ttgctaaccg tcataccggt tcggtagaga aatacaccga
    46801 agggcgcaaa atcgttcata tcgatattga gccgacgcaa attggtcgcg tgctgtgtcc
    46861 ggatctcggc attgtctctg atgctaaagc ggcgctgaca ctgctggttg aagtggcgca
    46921 ggagatgcaa aaagcgggtc gtctgccgtg tcgtaaagaa tgggtcgccg actgccagca
    46981 gcgcaaacgc actttgctgc gcaaaaccca tttcgacaac gtgccggtga aaccgcagcg
    47041 cgtgtatgaa gagatgaaca aagcctttgg tcgcgatgtt tgctatgtca ccaccattgg
    47101 tctgtcacaa atcgctgcgg cacaaatgct gcatgtcttt aaagaccgcc actggatcaa
    47161 ctgtggtcag gctggtccgt taggctggac gattccggct gcgctagggg tttgtgccgc
    47221 tgatccgaaa cgcaatgtgg tggcgatttc tggcgacttt gacttccagt tcctgattga
    47281 agagttagct gttggcgcac agttcaacat tccgtacatc catgtactgg tcaataacgc
    47341 ttatctgggg ctgattcgcc agtcgcagcg cgcgtttgat atggactact gcgtgcaact
    47401 cgctttcgag aatatcaact ccagtgaagt gaatggctac ggtgttgacc acgtaaaagt
    47461 agcggaaggt ttaggttgta aagcgattcg cgtcttcaaa ccggaagata ttgcgccatc
    47521 ctttgaacag gcgaaagcct taatggcgca atatcgggta ccggtagtcg tggaagttat
    47581 tctcgagcgt gtgaccaata tttcgatggg cagcgaactg gataacgtca tggaatttga
    47641 agatatcgcc gataacgcag cggacgcacc gactgaaacc tgcttcatgc actatgaata
    47701 agggagataa ataatgttac gtttctctgc taatttatcg atgttatttg gagaatatga
    47761 ttttctcgcc cgttttgaga aagctgcgca gtgtggtttt cgcggcgttg aatttatgtt
    47821 tccttatgac tacgacattg aagaattaaa acaggtgctg gcgagtaata aactcgaaca
    47881 tacgctgcac aatttaccgg cgggtgactg ggcggcgggg gagcgcggta ttgcctgtat
    47941 tcctggccgt gaagaagagt ttcgggatgg cgtagcagca gcgattcgtt atgcccgtgc
    48001 gctgggtaat aaaaaaatta actgtctggt cggtaaaacg ccggctggtt tcagcagtga
    48061 acagattcac gcaacgcttg tagaaaacct gcgttatgcc gcgaatatgc tgatgaaaga
    48121 agatatttta ttactgattg aacctattaa ccattttgat attcctggtt tccatctcac
    48181 cggaactcgg caggcgctga aattgattga tgatgttggt tgctgcaatt tgaaaattca
    48241 gtatgacatt tatcacatgc agcggatgga aggtgaatta accaacacca tgactcagtg
    48301 ggccgataaa attggccacc tgcaaattgc tgataatccg catcgcggcg aaccggggac
    48361 cggagaaatt aattatgatt atctctttaa ggtaatcgaa aattctgact acaacggttg
    48421 ggttgggtgt gaatataaac cccaaaccac cacggaagcc ggtttacgct ggatggatcc
    48481 gtaccgttaa aacgtaacgc tgttcaggca atgctttttt aggccgctaa gttggcaggg
    48541 gatcgtgttg tctgaattca ggaaaagcga aatttaaaag aggttaatta tgaaactggg
    48601 atttattggc ttaggcatta tgggtacacc gatggccatt aatctggcgc gtgccggtca
    48661 tcaactgcat gtcacaacca ttggaccggt tgctgatgaa ttactgtcac tgggtgccgt
    48721 cagtgttgaa actgcgcgcc aggtaacgga agcatcggac atcattttta ttatggtgcc
    48781 agatacacca caggttgaag aagttctgtt cggtgaaaat ggttgtacca aagcctcgct
    48841 gaagggcaaa accattgttg atatgagctc catttccccg attgaaacta agcgtttcgc
    48901 tcgtcaggtg aatgaactgg gcggcgatta tctcgatgcg ccagtctccg gcggtgaaat
    48961 cggtgcgcgt gaagggacgt tgtcgattat ggttggcggt gatgaagcgg tatttgaacg
    49021 tgttaaaccg ctgtttgaac tgctcggtaa aaatatcacc ctcgtgggcg gtaacggcga
    49081 cggccaaacc tgcaaagtgg caaatcagat tattgtggcg ctcaatattg aagcggtttc
    49141 tgaagccctg ctatttgctt caaaagccgg tgcggacccg gtacgtgtgc gccaggcgct
    49201 gatgggcggc tttgcttcct cacgtattct ggaagttcat ggcgagcgta tgattaaacg
    49261 cacctttaat ccgggcttca aaatcgctct gcaccagaaa gatctcaacc tggcactgca
    49321 aagtgcgaaa gcacttgcgc tgaacctgcc aaacactgcg acctgccagg agttatttaa
    49381 tacctgtgcg gcaaacggtg gcagccagtt ggatcactct gcgttagtgc aggcgctgga
    49441 attaatggct aaccataaac tggcctgata cccgcaataa aaatggccga tatcagaaaa
    49501 tgaatcggcc agcaatatta aaaaagaaag cagccaaaga tgttgcttca gtattaaaaa
    49561 taatattttt attttatttg ttcctcatag ctagattaaa acaacgttat tcgatacgtg
    49621 aaattaagag ggatttatgg aacatcagag aaaactattc cagcaacgcg gctatagcga
    49681 agatctattg ccgaaaacgc aaagccagcg gacctggaaa acatttaact attttacctt
    49741 atggatgggt tcggttcata acgttcccaa ttatgtgatg gtcggcggct tttttattct
    49801 cggcttgtct acctttagta ttatgctggc aattatcctc agcgcctttt tcattgccgc
    49861 ggtaatggta ttaaacggcg ctgcgggcag taaatacggc gtaccgtttg ccatgatcct
    49921 gcgtgcttct tacggcgtac gtggcgcact gtttcccgga ttattaagag gcggtattgc
    49981 tgccattatg tggtttggcc tgcaatgtta cgcggggtca ctggcctgct tgattctgat
    50041 tggcaaaatc tggccgggat ttttaactct cggtggtgat ttcacgctgt tagggctttc
    50101 tctaccgggc ttaattactt tcttactctt ctggctggtc aacgttggta taggttttgg
    50161 cggtggcaaa gttttaaata aattcactgc cattcttaac ccgtgcatct atatcgtttt
    50221 cggcggtatg gcgatttggg cgatttcgct ggtcgggctc ggtccaatct ttgactacat
    50281 tccgagcggt attcagaaag cagaaaacag tggattcttg ttcctggtgg tgattaacgc
    50341 ggtagttgcg gtctgggcgg caccggcggt gagcgcatcc gactttacgc aaaacgccca
    50401 ctcgtttcgt gagcaagcgc tggggcaaac gctgggttta gttgtggcct atattctttt
    50461 tgcggtcgct ggggtatgta ttattgccgg agccagtatt cactacggcg ctgacacctg
    50521 gaacgtgctg gatattgttc agcgttggga cagcctgttt gcctcgttct ttgcggtact
    50581 ggttattctg atgacgacta tttccactaa cgccaccggt aatattattc cggccggtta
    50641 tcagattgct gccattgcac cgacaaaact gacctataaa aacggcgtac tgattgccag
    50701 tattatcagc ctgctgatct gcccgtggaa attaatggaa aatcaggaca gtatttatct
    50761 gttcctcgat atcatcggcg gaatgctggg tccggtaatt ggtgtcatga tggcacatta
    50821 ttttgtggtg atgcgtgggc aaattaatct tgatgaactg tacaccgcac ctggcgatta
    50881 taaatattac gataacggtt ttaacctcac cgcgttttca gtaactctgg tggccgttat
    50941 tttatctctt ggcggtaagt ttattccctt tatggaaccg ttatcgcgtg tttcatggtt
    51001 tgtcggcgtc atcgtcgcct ttgcggccta cgccttatta aagaaacgta caacagcaga
    51061 aaaaacagga gagcaaaaaa ccataggtta attaatcccg atattgaaca ttgagttaaa
    51121 aaccaatctg tattttacaa ggagtttgtt atgtcttttg atttaatcat taaaaacggc
    51181 accgttattt tagaaaacga agctcgcgtt gtagatatcg ccgttaaagg cgggaaaatt
    51241 gctgctatcg gtcaggatct gggcgatgca aaagaagtta tggatgcgtc tggtctggtg
    51301 gtttcgccgg gcatggttga tgcgcacacc catatttctg aaccgggtcg tagccactgg
    51361 gaaggttatg aaaccggtac tcgcgcagcg gcaaaaggtg gtatcaccac catgatcgaa
    51421 atgccgctca accagctgcc tgcaacggtt gaccgcgctt caattgaact gaagttcgat
    51481 gccgctaaag gcaagctgac tatcgatgcg gcacaactcg gtggcctagt gtcttacaac
    51541 atcgaccgtc tgcatgagct ggatgaagtg ggcgttgtcg gcttcaaatg cttcgttgcg
    51601 acctgtggcg atcgcggtat cgacaacgac ttccgtgatg taaacgactg gcagttcttc
    51661 aaaggtgcgc agaagctggg cgaactgggg cagccggtgc tggtgcactg cgaaaacgcg
    51721 ctgatctgtg acgcactggg cgaagaagcg aaacgtgaag gtcgcgtaac tgcccatgac
    51781 tatgtggctt cgcgtccggt atttaccgaa gtggaagcga ttcgccgcgt gctgtatctg
    51841 gcgaaagttg ccggttgccg tctgcacgtt tgccatatca gcagcccgga aggcgttgaa
    51901 gaagtgactc gtgcacgtca ggaaggtcag gacgttactt gtgaatcctg cccgcattac
    51961 tttgtactgg ataccgatca gttcgaagaa atcggcactc tggcgaagtg ttcaccgccg
    52021 atccgcgatc tggaaaacca gaaaggcatg tgggaaaaac tgtttaacgg tgaaatcgac
    52081 tgcctggttt ccgaccactc accatgccct ccggaaatga aagccggcaa catcatggaa
    52141 gcatggggcg gtattgccgg tctgcaaaac tgtatggacg tgatgttcga tgaagcggta
    52201 cagaaacgcg gaatgtctct gccaatgttc ggcaaattaa tggcgactaa cgcagcagat
    52261 attttcggtc tgcagcaaaa aggccgtatc gccccaggaa aagatgccga cttcgtcttc
    52321 attcagccga atagcagcta tgttcttacc aatgacgatc tggaatatcg ccacaaagtc
    52381 agcccgtatg ttggccgtac cattggcgcg cgtatcacga aaaccatctt acgtggtgat
    52441 gtgatttacg acattgaaca gggcttccct gttgcgccga aaggtcaatt tatccttaaa
    52501 catcagcagt aatctggccc ctgcaatgcc cgtccttgcg gcgggcattc tccggttaag
    52561 gtgtgtttat gttcaatttt gcagtgagcc gcgaaagcct gttatcagga tttcagtggt
    52621 ttttctttat tttttgcaac acggttgtgg ttcctcctac gctactttct gcttttcagt
    52681 tgccgcaaag tagcctgctt acgctcacgc aatatgcttt tcttgctacc gcactggcct
    52741 gcttcgctca ggcgttctgc ggtcatcgtc gcgctattat ggaagggccg ggtggcctgt
    52801 ggtggggaac catccttact atcacccttg gcgaagcatc gcgcgggaca ccgatcaacg
    52861 atatcgctac cagcctggcg gtggggattg cgctctccgg cgtgctgacg atgttgattg
    52921 gttttagcgg attaggccat cgcctggcac ggttatttac gccgtcggtg atggtcttgt
    52981 ttatgttgat gctgggcgcg cagctgacca ctatcttttt caaaggtatg ctcgggctgc
    53041 cgtttggcat agccgaccca aattttaaaa ttcagttacc gccgttcgcg ctctcggtgg
    53101 cggtgatgtg cctggtactg gggatgatta tcttcctgcc gcaacgtttt gcccgttatg
    53161 gcctgctggt cggcaccata accggctggt tgttgtggta cttttgcttt ccttcttcgc
    53221 actcgctctc cggtgagttg cactggcagt ggttcccgct cggcagtgga ggtgctttgt
    53281 ctccgggaat tattctgacg gcggtgatta caggtctggt gaatatcagc aatacctacg
    53341 gtgcgattcg gggcacggat gttttttatc cgcagcaggg cgcggggaat acgcgttatc
    53401 gtcgcagctt tgtggcgacc ggatttatga cgctgataac cgtaccgctg gcggtaattc
    53461 cattttcacc gtttgtttca tccattggtt tattaaccca gacaggcgat tacacgcggc
    53521 gttcgtttat ttatggcagc gttatttgcc tgctggtggc gctggttccg gcactcacac
    53581 gactgttttg cagtatccct ttacccgtga gtagcgcggt catgctggtt tcttatctgc
    53641 ctttactctt ttccgcgctg gtgtttagcc agcaaataac ctttaccgct cgcaatattt
    53701 atcgactcgc attgccgtta tttgtcggca tatttttaat ggcattaccg cctgtgtatc
    53761 tgcaagacct tccattaacg ctgcgtcctc tgctcagtaa cggcttactg gtcggaattt
    53821 tactggctgt tcttatggat aatcttattc cgtgggaacg catcgaataa tttgttgaaa
    53881 aaggattgat aatgaagatt gtcattgcgc cagactcttt taaagagagc ttaagtgcag
    53941 aaaaatgttg tcaggcaatt aaagccgggt tttcgaccct ctttcccgat gcgaactata
    54001 tctgtttgcc gatagcggat ggcggcgaag ggacggtgga tgcgatggtc gccgcgacgg
    54061 gcggcaacat cgtgacgctt gaagtctgcg ggccgatggg cgaaacagtg aatgcttttt
    54121 atggccttac cggcgacggg aaaacggcgg tgattgagat ggcggcagca agtggcctga
    54181 tgctggtcgc gcctgaaaag cgtaatccgt tgctggcctc cagttttggt acgggggagt
    54241 taattcgtca tgcgctggat aacggcattc gccatattat tctcggcatt ggcggcagtg
    54301 cgacggtcga cggcggtatg ggcatggctc aggcgcttgg tgtgcgtttc cttgatgccg
    54361 acggccaggt gctggcggct aacggcggta atttagcgcg cgtggcaagc attgagatgg
    54421 atgaatgcga tccgcgtctg gcgaattgcc atattgaagt agcatgtgac gttgataacc
    54481 cgctggtagg ggcacgcggc gcggcggcgg tgtttggccc gcaaaaaggg gcaacgccgg
    54541 agatggtcga agaacttgaa caggggctgc aaaattacgc ccgtgtttta caacagcaaa
    54601 ctgaaattaa tgtctgccag atggcgggcg gcggcgctgc gggcggtatg ggtattgcgg
    54661 cggcggtatt cctcaatgcg gatattaaac cgggtattga gattgtactg cgggcggtca
    54721 acctggagca ggcggtgcag ggcgcagcac tggtgatcac cggggaaggg cgcattgact
    54781 cgcaaacggc aggcggtaaa gcgccgctgg gcgttgcgtc ggtggcgaag cagtttaatg
    54841 tcccggtgat tgggattgct ggcgtattgg gtgatggcgt ggaagtggtg caccagtacg
    54901 gcattgacgc ggtattcagc attttgcctc gtctggcacc tttagccgaa gtgctcgcca
    54961 gcggtgaaac caatctcttc aacagcgcgc gaaatattgc ctgcgccatt aaaataggtc
    55021 agggaattaa aaactgaccc ttacctttaa agcggatgcg atttatttcg catctttttt
    55081 tacctgaatt tactacaaaa aaagccacgg tataaaccgt ggcaaaatcc aacatagcta
    55141 aaaataatca ggcgagtggt atgacttaaa tctctacgtc gcggttgcaa tctttcgagt
    55201 aaatatagct gaacgcttca ccacgcccta cgccataacc agcctgtaaa gaataagcgc
    55261 ccataaagat gtaatcgcct tttttcaccg ggatccagtt attgtcgagg ttataaaccc
    55321 cctgaccgga aagaatatag gcaccgtgtt cctgaacatg tgtttcgata taaccgtggc
    55381 tggcacctgg tgcaaaagag aggatatgca tgttcatatc aaaacctaac tctttgggca
    55441 gaaaatccag caggatcaca tcatccatac cttcatagtg aatgcgttcc agttcgctgg
    55501 cattgccaga aaccagccac ggtgcatggc cttctaccgg aacatagcga cgcttatata
    55561 aaaagatttg gctgtcttcg gcctgggcgt taacaaacgt cattaaggag cctggtgggc
    55621 aataaagata gccaccttcg cttaaggcaa atgttttgcc ttcggctttg gcagtgatat
    55681 ttccagagat cacatacagg aacgtttcaa tgccttcgcc accgaagccc tgttggttgc
    55741 caccgttttg atgcagtgtg accagataat caacaaaaga ggcacccagc tttggcgtgg
    55801 agaggattgt cgcgtcacaa ttttcaaagc ccggaataat attttttacc agaccatccg
    55861 gggttaacag tgcgaaatta ccgtgtttaa caatcgcacg gttagccagt aaatcatcgc
    55921 ggtaaccggt gacgttattt aaatatccca tttatgactc cttatttctg ccaggcaagt
    55981 tgataaagca tgagtgccaa cgttttgacc ccttcggcaa ggtcggtaat attggtgcgt
    56041 tccgccgggt tatggctgat cccgttgatg ctgggaatga aaatcatgca ggttggtacg
    56101 cgaggcgcga aaatttgcgc gtcgtgcccg gcaccactgt gcatcacccg gtaattcagt
    56161 ttttctcttt cacacaattc tgtcagggtg gcgaccagct ccttattcat cggcacgggt
    56221 tcttcgtcca tccataaatc gatatcaata ccaatgtcca tttcatcgca aatcgcccgc
    56281 atgtcgtttt ctaactgttg ggtgaaatcg cgcagcacgg cagcgtcggt atgacgacaa
    56341 tcaatggtga acgtggtttt acccggcacc acatttaccg tattcgggcg cggctctact
    56401 ttgccaaagg tcagaaccag cggatcgccc atccttttcg ctttttcgac cgactgatgg
    56461 caaatgcgac tgaaagcgta aactgtatca cgacgataac ccatcggcgt ggtgcctgca
    56521 tggtttgatt cgccgttcag cgttaccgta taacgacgct gcccgacaat tgcattcacc
    56581 acgccaattg attgcccatt actttccagc acacagccct gttcaatatg cagttcaaca
    56641 aaggctttaa tatcctgacg cggagttagt ggggcgttcg gaagagtaaa tccgcaagcc
    56701 ttcatcgcat cgacaaaact atttcctttg gcatcacaga tattccgcac gtcgtcagga
    56761 ttcgccagcc caaagatatt tttactgccc cagaagacat acgggaagcg gctgccttct
    56821 tcttctgcca tcgccaccac ttcgaccgta cgtagcggcg cgccgtattg cgttttcagc
    56881 cagtcaattg ccagccacgc cgccagcgcg ccgaattgcc cgtcgaggtt accgccgtta
    56941 accacggtat cgatatgcga accgctcaga accacttctt gtggatattc ggtgccactc
    57001 aggcgaccat ataaattccc cacttcatcg aaacgtgttt ccagcccgct tgctgccatt
    57061 ctttttttaa attgctgctg agtttccagc cattccggcg aataaagtaa acgggtcata
    57121 ccacccgttg ggtcagcgcc aaaagaggaa agccagggca gcgtttcttc tatggcttga
    57181 cgaaaatgtg taatcataag aaagtcctgt ctcaataatt attgcgcaaa gggatttttc
    57241 gtttcgtatg acgtgttata aagcgcgtcg gaaattaaat actggtaaat atcatcaaca
    57301 atttcgatgc cttcgacggc ggctttgcgt tgtttaatat cctgatcctg tccgggataa
    57361 taaacctgat taaaaccggg cgcgggggta atggcattta attcgcgcat ggtctggcta
    57421 agatgttgac gaaataattt gctggagaag aaaaagttcg ggttaataac gatatgtaat
    57481 tgccccaaat tacgcccggc gtgtaaatcg tcatacatcg aactaacctg tcgcccgaac
    57541 ggtaagccga gtaagacgcc tgagaggacg tcaatcatca tcatcaggcc ataccctttt
    57601 ggcccggcgg cggggagcag agcatgtacc gcgaacggat cggttgttgg tgcaccgttt
    57661 ttatcgaccg cccaggtatc cgggatagac atattacgcg agcgggcgtc gagcactttt
    57721 ccccatgcct gtacggtagt cgccatatca aaggtaagga tctcgtcgcc ttctcccggc
    57781 gcggcaaagg ccagtgggtt agtaccgtag taaatttccg cgccgccaaa cggcaccacc
    57841 attggatcgg actggcacat cgaaatacca attaatccgg cgcgggctgc ctgctgcaca
    57901 aaataagaga ttgcgccgct gtgacccatc cggctgatac cgaccaccgc aacgccattt
    57961 tgctgggcgg ttttgatggc atgttccata cccattttcg ccgcgacctg tccggcggca
    58021 ttgtcggcat gtaaaattgc cgagcacggc ccggtttcct caagacgaaa ctccggttcg
    58081 cggttggtgc cgccttttga aatgcgttcc gcgtagtatt ccacgcgcac cgcgccatga
    58141 gagtggatcc ctctggcatc ggcgtaaacc aatacttcag ccacggttgc agcgtgctca
    58201 cgttttaacc cagcctggca gagtttattc tcaattagct ggtggagtgt ttcccgactg
    58261 attttcatct gtcttccttt ttaacgacgg tgtgaagcat gactgcaatt aacatacagg
    58321 gaaaatatct ggattatgtg atccagacag gcaaaaaaat atagttagaa tttatttgat
    58381 aatccgctc
//
LOCUS       DAFAVF010000012        57236 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFAVF010000012 DAFAVF010000000
VERSION     DAFAVF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.012"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..483
                     /locus_tag="LGK42_000703"
     CDS             <1..483
                     /locus_tag="LGK42_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2656983.1"
                     /translation="TFKNTNRIYLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGY
                     NFTGSGWWFKPFFAKRYTDQTYYTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDR
                     DATYAAGNGGKEGLNGAVALWWNATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF
                     "
     gene            complement(522..1448)
                     /locus_tag="LGK42_000704"
     CDS             complement(522..1448)
                     /locus_tag="LGK42_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2656984.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1538..2536
                     /locus_tag="LGK42_000705"
     CDS             1538..2536
                     /locus_tag="LGK42_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBK2656985.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2533..2751)
                     /locus_tag="LGK42_000706"
     CDS             complement(2533..2751)
                     /locus_tag="LGK42_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBK2656986.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2753..4768)
                     /gene="ligA"
                     /locus_tag="LGK42_000707"
     CDS             complement(2753..4768)
                     /gene="ligA"
                     /locus_tag="LGK42_000707"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK2656987.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4839..5825)
                     /gene="zipA"
                     /locus_tag="LGK42_000708"
     CDS             complement(4839..5825)
                     /gene="zipA"
                     /locus_tag="LGK42_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBK2656988.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6055..6816
                     /gene="cysZ"
                     /locus_tag="LGK42_000709"
     CDS             6055..6816
                     /gene="cysZ"
                     /locus_tag="LGK42_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBK2656989.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7001..7972
                     /gene="cysK"
                     /locus_tag="LGK42_000710"
     CDS             7001..7972
                     /gene="cysK"
                     /locus_tag="LGK42_000710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK2656990.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8356..8613
                     /gene="ptsH"
                     /locus_tag="LGK42_000711"
     CDS             8356..8613
                     /gene="ptsH"
                     /locus_tag="LGK42_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBK2656991.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8658..10385
                     /gene="ptsI"
                     /locus_tag="LGK42_000712"
     CDS             8658..10385
                     /gene="ptsI"
                     /locus_tag="LGK42_000712"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBK2656992.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10426..10935
                     /gene="crr"
                     /locus_tag="LGK42_000713"
     CDS             10426..10935
                     /gene="crr"
                     /locus_tag="LGK42_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK2656993.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10977..11828)
                     /gene="pdxK"
                     /locus_tag="LGK42_000714"
                     /gene_synonym="thiJ"
     CDS             complement(10977..11828)
                     /gene="pdxK"
                     /locus_tag="LGK42_000714"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBK2656994.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11933..12301
                     /locus_tag="LGK42_000715"
     CDS             11933..12301
                     /locus_tag="LGK42_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBK2656995.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12304..13215)
                     /gene="cysM"
                     /locus_tag="LGK42_000716"
     CDS             complement(12304..13215)
                     /gene="cysM"
                     /locus_tag="LGK42_000716"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBK2656996.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13349..14446)
                     /gene="cysA"
                     /locus_tag="LGK42_000717"
     CDS             complement(13349..14446)
                     /gene="cysA"
                     /locus_tag="LGK42_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBK2656997.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14436..15311)
                     /gene="cysW"
                     /locus_tag="LGK42_000718"
     CDS             complement(14436..15311)
                     /gene="cysW"
                     /locus_tag="LGK42_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBK2656998.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15311..16144)
                     /gene="cysT"
                     /locus_tag="LGK42_000719"
     CDS             complement(15311..16144)
                     /gene="cysT"
                     /locus_tag="LGK42_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBK2656999.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16144..17160)
                     /gene="cysP"
                     /locus_tag="LGK42_000720"
     CDS             complement(16144..17160)
                     /gene="cysP"
                     /locus_tag="LGK42_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBK2657000.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17331..18122)
                     /gene="ucpA"
                     /locus_tag="LGK42_000721"
     CDS             complement(17331..18122)
                     /gene="ucpA"
                     /locus_tag="LGK42_000721"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBK2657001.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18251..19108)
                     /gene="murR"
                     /locus_tag="LGK42_000722"
     CDS             complement(18251..19108)
                     /gene="murR"
                     /locus_tag="LGK42_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBK2657002.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19272..20168
                     /gene="murQ"
                     /locus_tag="LGK42_000723"
     CDS             19272..20168
                     /gene="murQ"
                     /locus_tag="LGK42_000723"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK2657003.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            20172..21596
                     /gene="murP"
                     /locus_tag="LGK42_000724"
     CDS             20172..21596
                     /gene="murP"
                     /locus_tag="LGK42_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBK2657004.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21601..22905
                     /gene="pbp4b"
                     /locus_tag="LGK42_000725"
     CDS             21601..22905
                     /gene="pbp4b"
                     /locus_tag="LGK42_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBK2657005.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(22963..23862)
                     /gene="yfeX"
                     /locus_tag="LGK42_000726"
     CDS             complement(22963..23862)
                     /gene="yfeX"
                     /locus_tag="LGK42_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBK2657006.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23958..24533)
                     /locus_tag="LGK42_000727"
     CDS             complement(23958..24533)
                     /locus_tag="LGK42_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBK2657007.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24594..25043)
                     /locus_tag="LGK42_000728"
     CDS             complement(24594..25043)
                     /locus_tag="LGK42_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBK2657008.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25030..25455)
                     /locus_tag="LGK42_000729"
     CDS             complement(25030..25455)
                     /locus_tag="LGK42_000729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBK2657009.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25669..26538
                     /gene="amiA"
                     /locus_tag="LGK42_000730"
     CDS             25669..26538
                     /gene="amiA"
                     /locus_tag="LGK42_000730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBK2657010.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26542..27441
                     /gene="hemF"
                     /locus_tag="LGK42_000731"
     CDS             26542..27441
                     /gene="hemF"
                     /locus_tag="LGK42_000731"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBK2657011.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27447..28499)
                     /gene="eutR"
                     /locus_tag="LGK42_000732"
     CDS             complement(27447..28499)
                     /gene="eutR"
                     /locus_tag="LGK42_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBK2657012.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(28545..29045)
                     /gene="eutK"
                     /locus_tag="LGK42_000733"
     CDS             complement(28545..29045)
                     /gene="eutK"
                     /locus_tag="LGK42_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBK2657013.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29058..29717)
                     /gene="eutL"
                     /locus_tag="LGK42_000734"
     CDS             complement(29058..29717)
                     /gene="eutL"
                     /locus_tag="LGK42_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBK2657014.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29727..30614)
                     /gene="eutC"
                     /locus_tag="LGK42_000735"
     CDS             complement(29727..30614)
                     /gene="eutC"
                     /locus_tag="LGK42_000735"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBK2657015.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRLRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30635..31996)
                     /gene="eutB"
                     /locus_tag="LGK42_000736"
     CDS             complement(30635..31996)
                     /gene="eutB"
                     /locus_tag="LGK42_000736"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBK2657016.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(32008..33411)
                     /gene="eutA"
                     /locus_tag="LGK42_000737"
     CDS             complement(32008..33411)
                     /gene="eutA"
                     /locus_tag="LGK42_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBK2657017.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(33408..34634)
                     /gene="eutH"
                     /locus_tag="LGK42_000738"
     CDS             complement(33408..34634)
                     /gene="eutH"
                     /locus_tag="LGK42_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBK2657018.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(34751..35938)
                     /gene="eutG"
                     /locus_tag="LGK42_000739"
     CDS             complement(34751..35938)
                     /gene="eutG"
                     /locus_tag="LGK42_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBK2657019.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(35928..36764)
                     /gene="eutJ"
                     /locus_tag="LGK42_000740"
     CDS             complement(35928..36764)
                     /gene="eutJ"
                     /locus_tag="LGK42_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBK2657020.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(36775..38178)
                     /locus_tag="LGK42_000741"
     CDS             complement(36775..38178)
                     /locus_tag="LGK42_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBK2657021.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(38190..38477)
                     /gene="eutN"
                     /locus_tag="LGK42_000742"
     CDS             complement(38190..38477)
                     /gene="eutN"
                     /locus_tag="LGK42_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBK2657022.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(38584..38877)
                     /gene="eutM"
                     /locus_tag="LGK42_000743"
     CDS             complement(38584..38877)
                     /gene="eutM"
                     /locus_tag="LGK42_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBK2657023.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(38916..39932)
                     /gene="pta"
                     /locus_tag="LGK42_000744"
     CDS             complement(38916..39932)
                     /gene="pta"
                     /locus_tag="LGK42_000744"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK2657024.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATSIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(39929..40732)
                     /gene="eutT"
                     /locus_tag="LGK42_000745"
     CDS             complement(39929..40732)
                     /gene="eutT"
                     /locus_tag="LGK42_000745"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBK2657025.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(40729..41430)
                     /gene="eutQ"
                     /locus_tag="LGK42_000746"
     CDS             complement(40729..41430)
                     /gene="eutQ"
                     /locus_tag="LGK42_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBK2657026.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(41405..41884)
                     /gene="eutP"
                     /locus_tag="LGK42_000747"
     CDS             complement(41405..41884)
                     /gene="eutP"
                     /locus_tag="LGK42_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBK2657027.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(41897..42232)
                     /gene="eutS"
                     /locus_tag="LGK42_000748"
     CDS             complement(41897..42232)
                     /gene="eutS"
                     /locus_tag="LGK42_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBK2657028.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(42525..44804)
                     /gene="maeB"
                     /locus_tag="LGK42_000749"
     CDS             complement(42525..44804)
                     /gene="maeB"
                     /locus_tag="LGK42_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBK2657029.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            45093..46043
                     /gene="tal"
                     /locus_tag="LGK42_000750"
     CDS             45093..46043
                     /gene="tal"
                     /locus_tag="LGK42_000750"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBK2657030.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            46063..48066
                     /gene="tkt"
                     /locus_tag="LGK42_000751"
     CDS             46063..48066
                     /gene="tkt"
                     /locus_tag="LGK42_000751"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK2657031.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTAGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(48162..49205)
                     /locus_tag="LGK42_000752"
     CDS             complement(48162..49205)
                     /locus_tag="LGK42_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBK2657032.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNVLLDYGNWRMNGLRCSL
                     DPLRREANVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(49331..49906)
                     /gene="nudK"
                     /locus_tag="LGK42_000753"
     CDS             complement(49331..49906)
                     /gene="nudK"
                     /locus_tag="LGK42_000753"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBK2657033.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(49974..51953)
                     /gene="aegA"
                     /locus_tag="LGK42_000754"
     CDS             complement(49974..51953)
                     /gene="aegA"
                     /locus_tag="LGK42_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBK2657034.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            52159..53859
                     /gene="narQ"
                     /locus_tag="LGK42_000755"
     CDS             52159..53859
                     /gene="narQ"
                     /locus_tag="LGK42_000755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBK2657035.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            54023..57136
                     /gene="acrD"
                     /locus_tag="LGK42_000756"
     CDS             54023..57136
                     /gene="acrD"
                     /locus_tag="LGK42_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBK2657036.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
BASE COUNT        13183 a        15864 c        14781 g        13408 t
ORIGIN      
        1 acctttaaaa ataccaaccg tatttacctg ggtgacaccg gatttaatct ctacttgcat
       61 gcgtatggca cctacggttc tgcgaatcgc gtgaatttcc acgacgatat gttcctgtac
      121 ggtatcggct acaatttcac cggcagcggt tggtggttca aaccgttctt tgctaagcgt
      181 tatacagatc aaacctatta cactggcgac aacggctatg tcgccggctg ggttgcaggt
      241 tacaacttta tgctgggcag cgagaaattc actcttacca actggaacga gtacgagttt
      301 gaccgtgacg ctacctatgc agcgggtaat ggcggtaaag aagggctgaa tggcgcagtt
      361 gcactgtggt ggaatgcaac atcacacatt actacgggga ttcagtatcg atatgcggat
      421 gacaaactgg gcgaagattt ttaccaggat gcgataatct actccatcaa atttaacttc
      481 tgattgatta ctgccggggc tcccggcagt atgaattcac tctatatctg atacaacgga
      541 tccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
      601 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca
      661 accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag cgccagtgcg
      721 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
      781 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
      841 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
      901 acataatctt cgacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
      961 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     1021 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac
     1081 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     1141 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     1201 tcgcgcaacg tgctgttcag ttcatccagc agtcgctcaa gacgcaaagc cagctgctgc
     1261 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     1321 ttttccagtt ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct
     1381 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     1441 taattcatct gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga
     1501 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     1561 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     1621 cgatttcgtc cccttctttg aaaatctgac caccgcagcg attgccctgc tgttctttat
     1681 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
     1741 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
     1801 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     1861 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
     1921 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt
     1981 tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat
     2041 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
     2101 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
     2161 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
     2221 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
     2281 ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg
     2341 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
     2401 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
     2461 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcacagc aggaaagcag
     2521 cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
     2581 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga attcatcttt
     2641 gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttatattt
     2701 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
     2761 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgctttcg
     2821 ccagtttaga acctgccgct tctccggcaa tcaccagatc ggttttcttc gacacgctgc
     2881 ccgcgacttt cgcgcccagt tcaaccagtc gagctttagc gtcatcacgc gacatctggc
     2941 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
     3001 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
     3061 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
     3121 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
     3181 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
     3241 gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgattttg
     3301 gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag
     3361 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
     3421 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgct ccgcaaatca
     3481 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
     3541 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
     3601 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
     3661 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
     3721 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
     3781 gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgctaccg
     3841 cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct
     3901 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
     3961 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc
     4021 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
     4081 gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
     4141 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
     4201 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
     4261 cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatagcg cgaatagtac
     4321 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
     4381 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
     4441 aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
     4501 tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga
     4561 aagccgccag cggcgcagcg cctacacgtt gggtaggcga atcaggcgta atcagttctg
     4621 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
     4681 tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt
     4741 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
     4801 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
     4861 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
     4921 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
     4981 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatggtgac acccggagta
     5041 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
     5101 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
     5161 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
     5221 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
     5281 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
     5341 tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa
     5401 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc
     5461 gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga
     5521 cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg acgagcagcc
     5581 tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg
     5641 ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt
     5701 tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt
     5761 accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc
     5821 atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag
     5881 agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca caaagataga
     5941 tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc
     6001 cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt
     6061 tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc
     6121 gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg
     6181 atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc gactctcatg
     6241 agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc
     6301 tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg
     6361 tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac accgccagat
     6421 accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa
     6481 tttgcctggt atctgccgcg cgcaattgta ttgctaattc tttacttaat ccctggtatt
     6541 gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag
     6601 tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg
     6661 cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc
     6721 ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc
     6781 gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac
     6841 cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt
     6901 tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc
     6961 tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga
     7021 taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg
     7081 cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc
     7141 caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga
     7201 accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa
     7261 actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt
     7321 aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc
     7381 agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc
     7441 ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg
     7501 tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg
     7561 ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga
     7621 ttctccagtt atcgcccagg cactggctgg tgaagagatt aaacctggcc cgcataaaat
     7681 tcagggtatt ggcgctggtt ttatcccagc taacctcgat ctcaagctgg tcgataaagt
     7741 cattggcatc accaatgaag aagcgatttc taccgcgcgt cgtctgatgg aagaagaagg
     7801 tattcttgca ggtatctctt ctggagcagc tgttgctgcg gcgttgaaac tacaagaaga
     7861 tgaaagcttt accaacaaga atattgtggt tattctaccg tcatcgggtg agcgttattt
     7921 aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ctgcaacagt aatgccagct
     7981 tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt
     8041 tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt
     8101 tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt
     8161 tcaattcttc ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc
     8221 cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa
     8281 caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata
     8341 agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac
     8401 acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg
     8461 acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg
     8521 actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa
     8581 catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc
     8641 acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta
     8701 aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc
     8761 aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg
     8821 aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag
     8881 ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag
     8941 ataagcacat gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc
     9001 tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta
     9061 agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg
     9121 aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga
     9181 aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc
     9241 gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg
     9301 acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag
     9361 ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta
     9421 aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagtg tgcgctaaca
     9481 ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt
     9541 atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg
     9601 ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg
     9661 acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc
     9721 tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc
     9781 gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct
     9841 ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg
     9901 acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg
     9961 ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg
    10021 atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc
    10081 agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg
    10141 aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt
    10201 tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga
    10261 agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc
    10321 aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct
    10381 gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat
    10441 aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga gatcattgct
    10501 ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa
    10561 atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac
    10621 ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc
    10681 gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag
    10741 cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg
    10801 ccgctactgg aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac
    10861 gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc
    10921 cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat
    10981 gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc
    11041 acttccagta cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc
    11101 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc
    11161 cgtgaatggg aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga
    11221 ttttcttcat taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga
    11281 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc
    11341 agctcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc
    11401 tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc
    11461 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    11521 atttgcgatg ccgttcccat atagcccgtg gttacagcgc gcagctggcg cagcgcatca
    11581 cgctcctgaa gcgcacgcaa ataaccgcta aaccattcat ccggaatcgc accaccgtag
    11641 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa aacattcaga
    11701 ccgttctgtt tgatagcagg cacggcaatg ctgttgccca cgctgccgta aaccacctgc
    11761 gactgcacgg cgacgatatc cgcctgcagc gccctgctct tatcgttaaa caacaacaaa
    11821 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta
    11881 cctgattctg gcagtcaaat cggctatcac aaaacaagga taaggtaatt caatgaagaa
    11941 aatcatttgt ctggtcatta cactactaat gacactcccc gcttacgcga agttaactgc
    12001 ccatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    12061 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttctcatc tgcgtctgaa
    12121 gctgggcaat acccgtaacc gcattgacac tgccgagcag tttattgata aggttgcttc
    12181 gtcgtcatcg attaccggga agccgtatat tgtgaagatc cccggtaaga gcgatgagaa
    12241 cgcacagcct tttttacatg cgctaattgc gcagacggat aaaacggtgc ctgcgcaata
    12301 atcttaaatc cccgccccct ggctaaaatg ctcttcccca aacaccccgg tggaaaggta
    12361 gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag cttttgccac
    12421 ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca cagaatattc cttcccgcac
    12481 tgccagttcg cgcatggtgt tttctgcatc gcgctgatga atatccagca cctcatccac
    12541 cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa tgccgggaat
    12601 actgctgcct tcttccggct gcaggccaac aatcgttact ggtttggatt gttcgcgcat
    12661 aaagcgtgaa acgccggtga tagtgccggt tgttcccatg ctggagacaa aatgggtaat
    12721 gcgcccgccg gtttgctgcc agatttccgg cccggtagtg gtgtaatggg cgtaagggtt
    12781 atcgggatta ttaaactgat cgagcagctt tccttcgcca cgattcgcca tctccagcgc
    12841 cagatcgcgc gcaccttcca tgccctgctc tttggtgaca agaatcagtt ccgcaccata
    12901 agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg
    12961 atagcctttc agcgcggcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc
    13021 gattaagaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga tcatcgaaag
    13081 tgccgcacga tctttcaccg aacctgccgg gttattgcct tccagtttta accacacttc
    13141 actgccgtta tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg
    13201 ttctaatgta ctcacgatct ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt
    13261 cttatcaggt ctacaggtta caaaccttgc cattaaaaag cccggatcgc gggaagcgcc
    13321 tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca agttcctcat
    13381 ctcgggtttc gatacgctcg tcgccgttat acagccgcgc atgttgcaga ccaacgaata
    13441 aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt
    13501 accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg gcttccagta
    13561 cctgtaccgg cagcggcgaa tcgagactgg tacggcggct gatatccact tcccacgggc
    13621 gcaggaagag atccaccgga ccctgatacg caggtgtgta gcccagcggc cagcgatgcg
    13681 cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact tcgcccataa
    13741 attcgagtac aaaacgggtc gctggttcgc gccatacctg attcggcgca tcagcctgtt
    13801 caatattccc ctgactcatc accactacac gatcggctac ttcggtcgcc tcttcctggt
    13861 cgtgggtcac gaacacgctg gtgaatttca gctcttcatg cagttgacgc agccagcgac
    13921 gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc agcagaattt
    13981 gcggttctac agccagcgcg cgcgccagcg ccacgcgctg tttctggccg ccggaaagct
    14041 gtgccggata acgatctgcc agatgagcaa gctgcaccat ctccagcaat tttgtcactt
    14101 tcgctttgat ggctgcggca ttcgggcgct cgcgacgtgg cagcaccgtc aggccaaaag
    14161 cgatattgtc gaacaccgtc atatggcgga acagcgcgta atgctggaac acgaaaccga
    14221 ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc gtggaagcga atatgcccgc
    14281 tggtttgatg ctccagcccg gcgataatgc gcagcagcgt ggttttcccg gaaccggacg
    14341 gccccagcaa cgcgaccatc tgacctgaag gaatatccag tgagatatcg ttcagcacct
    14401 gggtgcgacc aaacgacttc ttaatattgg caatctcaat gctcatgatg ttcctcctgc
    14461 tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactttttaa aaacagggtg
    14521 ataatcgcca tcagcgttaa cagcgccgca gcggtaaagg agccgacggt gttgtagtcc
    14581 tgctccagca attcaatctg taacggcagc gacagggttt cgccgcgaat cgagccggaa
    14641 accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt tggtcaacac cacgccataa
    14701 agcagcgccc agcggatgtt cggtaatgtg acgcgacgga acatctgcca gccggacgcg
    14761 ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc ttaacatcac cggcaccagt
    14821 tcgcgcacca caaacggaca cgtcacgaag atggtgacca gcaccattcc cggccaggag
    14881 aacataattt gcaggttatg ctcgtcgagc caaccgccga gcgggccgtt agagccgtag
    14941 aacagcaaat acaccagacc ggcaaccacc ggcgacacgg caaacggaat gtccagcagc
    15001 gtcagcagca actggcgtcc ggggaagtta aagcgcgtca ccagccacgc cagtagaata
    15061 ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag ccagatggcg
    15121 tgcagcatat ccggatcggc cagattctgt aaaacaggca tcagcccttt gctgaacgcc
    15181 tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgataccag catcccgatg
    15241 ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt caattgtgtc
    15301 acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga gtgttaattg
    15361 agaacagcag tatcagggat gccgcgagga tcaccgaagc aatcgcgctc gccgccgggt
    15421 aatcaaactc ctgcaagcgc acaaaaatca tcagcgacgt cacttccgtc ttccacgcga
    15481 tatttccggc gataaaaatc accgcgccaa attcaccaag actacgggta aacgacagcg
    15541 ccacgcccgc caccagcgcc ggagaaagct ccggcagcac cactttgcag aaactctgcc
    15601 agcgcgttgc accaagcgtt tccgccgctt cttcatattc cgggcctaac tcttccagca
    15661 ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc accgcaatcc
    15721 ccagccatgt ataggtgact ttgatatcaa acttcgccag ccattcaccg taaaaaccgt
    15781 ttacggaaaa gagcgaggcc agcgttaaac cggcgacagc cgttggcagc gcaaagggta
    15841 aatccatcag cgcatcaagc agcgtgcggc ctgggaagcg atagcgggtt aggatccacg
    15901 ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac agcagcgtta
    15961 ctttgtaggc cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc gcccagctca
    16021 tctgggccag ttgcatcacc agcgcggaga gcggcagcag caaaatcagg cacacaaaca
    16081 gcagactggt gccgaggctt aaggtaaagc ccggcagcac gcgtctggag gagacagcaa
    16141 acatcagtta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt
    16201 tttcatcact tccggccagg agccaaattt gtcttccacg cggaacagct cggtctgcgg
    16261 gaatttgtct ttcagtttgt ccatcacctc cgggttattc acgcggtagt aatagtcggt
    16321 gatgatggtt tgcgcctgcg ggctatagag ccagttcaga taggctttgg cggctttttc
    16381 cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc gggaattccg ccagaatgtt
    16441 ggttttcgga atcaccactt caaagccctg cgcttcatac tgtttacgga tgttgttcac
    16501 ttccgattcg aagctaatca gcacatcgcc caggccacgc tcggcaaaag tggtggttgc
    16561 gccacgaccg ccggtatcga acacttcaac gtttttcagg aactgggtca taaactgttc
    16621 ggttttggct ttatcgccac cgtcagcttt atccgctgcg ccccatgccg ccagataggt
    16681 ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac
    16741 caggtcgttc caatcgtgga tattcttcgg gttaccctta cgcaccagga agcccatggt
    16801 ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtcggccg ggatcagctt
    16861 gcctttatcg tgcaggattt gtacgtcggt cacctggtta taagtgacaa cgtcggcttt
    16921 taagccctgt aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt gttttatcgt
    16981 cagtttgtcg ccgccgttat cttttgccca ttgttgctca aacggcggat tcagggcggc
    17041 aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct gtacatggcc
    17101 cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt taacggccat
    17161 tgcgcaccct tataaattta atgactttct aatagccatc atatttataa cggacgtgaa
    17221 aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga ataagaaccg
    17281 ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa cagaggtgaa tcagataccg
    17341 acgctaaccg tctccggcag tgtgctgccg ccatcaatca cattctgtgt accggttaaa
    17401 tagctggatt catccgatgc gaggaaggcc gccagttcgc cgacttccag cggatcggcg
    17461 aggcgacgca tcgggattgc tttcgccatt tcagtcagca ccgactctgg atcttccggg
    17521 ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca cgtatcccgg gcaaatggcg
    17581 ttaacgcgaa taccagactg cgcgtactcc accgccagcg attttgtcag gccaacaatc
    17641 gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg ccaccatatc gccagtgacc
    17701 gaagacatca tcacaatgcg accatctttg cgggcaatca tctccggcag caccgccttc
    17761 gtgacgttcc atacgccttt aatattgatg tcaatatgga aatcgcgatc gtcatcgctc
    17821 atatcgagga aactgcccag acgacaaacg cctgcgttat tcaccaggat atcaatgcgc
    17881 ccttcttttt ccttcgcgcg tttgatagct gcggctaccg acgccgggtc acgcacatcg
    17941 gcgacaaccg ccgtacagcg atgaccacga ccacacagtt cgtccgccag cttttcgatc
    18001 tcaggggaga tatccagcaa gattaggttt gcgccatgac gcgcaaaagt tctggcaatt
    18061 ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc
    18121 attttaatgc tctccttgtt aatacagagc gttacactcc attccctctg aaaatacagg
    18181 ataaacaaaa tattgctgcg gatcagctca ctaattcagg aaaatctcaa tccagtcgct
    18241 gctttatgca tcatttcagg cgttgtgtta gctcactgct gcgctgaatc attttcaatg
    18301 actccacatc attgagttgc acaagaccaa caaacagtaa atcggtaaca gaattttgcg
    18361 ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg gtatcgagcg
    18421 tgaaatgcgc cagccgccgc agcggagaat cagccagaga agtaatggca atcaccgttg
    18481 ccccctgctt tcgtgccgct tctgcgcata agacaatctc ttttttactg ccgctgtaag
    18541 agatagcgat ttgtacatcg ccttttttca acgcctggga aacagtggct tgtacgtgcg
    18601 tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac agatcgcgcc
    18661 ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta ctgatgacct
    18721 caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc agcgccagct
    18781 ctttttcgcg attcaatttg cgggcaatca cttccagcga atcatcactg gtaatcgaac
    18841 tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat tcgcctatta
    18901 acgccatacg caattcagta aatccttgcg cgccgagttt ttgggcaaat ttcactatgc
    18961 tcgactggct aatgccgagc tgtttcgcca tctgccgtga agaaacggat tgcagctcac
    19021 tcacgttggc ctgcagaaag tcagcgattt tttgctcgtt ttcggtgaat tcactccccg
    19081 cattgcttat tttcgtcagg tacaacatct tccactctcc tgggctggca gcataaattc
    19141 cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg attcctttaa
    19201 gtctgtcaat tagggatata tgattccata catctgcaat tatttgatcg taaatagtaa
    19261 ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc gcctcggctg
    19321 aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa gataaaaccg
    19381 taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc ggcgatcgat gttatccacg
    19441 cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg
    19501 ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt ctggtggttg
    19561 gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggcgct gaagatagcc
    19621 gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat gtggtggttg
    19681 gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac gcacgccagc
    19741 tcggctgccg cacagtggga atttcctgta atccggggag tgccgtttcc accaccgccg
    19801 agtttgccat tacaccgatt gtaggtgccg aagtggtcac tggttcttcg cgaatgaaag
    19861 caggcacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg attaaatctg
    19921 gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc
    19981 gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg gaagcggcgt
    20041 taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg
    20101 ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag gttttagaca
    20161 aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt cttacccgtg
    20221 tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg
    20281 tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg aagggcgtca
    20341 ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa
    20401 aagcgatgag cgagctgctg ggcgaggcac cggtacagga tgccgcagaa atcgccgccc
    20461 agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat
    20521 tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg ctgctgggga
    20581 tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg
    20641 acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt cactttcctg gtgattctgg
    20701 tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca attattgccg
    20761 cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc ggttttcacg
    20821 atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg attgccgcgt
    20881 gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat ctcgacatgc
    20941 tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg atcattatgc
    21001 cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg aacagtaatc
    21061 cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc
    21121 agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac agcttatttc
    21181 ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc tactggcggg
    21241 cgcaaccgca cagtgcatta cgcagtcagg tacgcggggc gattattccc ggtctgctgg
    21301 gcgttggtga accgttgatt tacggcgtca ccctgccccg catgaaaccg tttattaccg
    21361 cctgtttagg cggcgcagcg ggcggtttgt ttatcggcct gatagcctgg tggggtctgc
    21421 cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg ctgatgactt
    21481 ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg gtggcatggg
    21541 tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg gactgatatc
    21601 atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt cagcgccgca
    21661 aaataccctg ttctgacaga aagttcgcca gagaaagcag ggttcaacgt cgaacggctt
    21721 aaccagatgg atcgctggat tagccagcaa gtggacgccg gttatcccgg cgtaaacctg
    21781 ctgatcatta aagataatca gattgtgtat cgcaaagcct ggggagcggc gaaaaagtac
    21841 gatggcagcg tgttgatgga acagcctgtc aaagccacca ccgggacgct gtatgacctg
    21901 gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc aaaagctgat gagcgaaggc
    21961 aaactgcatc ctgacgatcg gattgcgaaa tatattccgg ggtttgccga tagtccaaat
    22021 gacaccatca aaggcaaaaa cacgctgcgg atttctgacc tgctgcatca cagcggcggt
    22081 ttcccggcgg acccacaata tccgaataaa gcggtcgcgg gcgcgttata ttcccaggat
    22141 aaaggccaga cgctggagat gatcaagcgc acgccgctgg agtatcagcc cggcagcaag
    22201 catatttaca gtgatgtcga ttatatgctg cttggattta tcgtcgagtc ggtcaccggt
    22261 cagccgcttg accgctatgt tgaagagtcg atttatcgcc cgctcggcct gacacatacg
    22321 gtatttaacc cgctgctgaa aggctttaaa ccgcagcaaa ttgccgccac ggaattgaac
    22381 ggcaataccc gcgatggcgt gatccatttc ccgaatatcc gcacctccac tctctggggt
    22441 caggtgcacg atgaaaaagc cttttattcg atgggcggcg tttccgggca cgcaggtttg
    22501 ttttccaata ccggcgatat tgcggtgtta atgcaaacga tgctgaacgg cgggggctat
    22561 ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa
    22621 gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt
    22681 ggcacgctgg caagcccgca aacctacggt cacactggct ggaccggaac ggtgaccgtt
    22741 atcgatccgg tgaatcatat ggcgattgtg atgttaagca acaagccaca ttcgccggtt
    22801 gccgatccgc aaaagaatcc caatatgttc gaaagcggtc agctgccgat tgcaacgtat
    22861 ggttgggtag ttgatcaggt gtatgcggcg ttaaagcaaa agtaataaaa aggccagtca
    22921 gtaatgttac tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt
    22981 gtccagcgac ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc
    23041 atcacgctta ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag
    23101 acgcgcgcag taggcgcaga agtacagacc gtgagtgcca ctggcagtgc cgtacggcag
    23161 gctctggcga acaatcttca gccctttgcc atcttctttc agatcaacgc gggtgaggtg
    23221 agaggtttcc ggacgttcgt cgccgtcgat ctcttcgttg gcctctttgg tgcgcccgat
    23281 catcatctcc tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gttcccaacg
    23341 ctgcacaaac acatagctgc cgcccgcatc cacgccgtct ttgataaccg ccacttcgcg
    23401 acgcgtctct tcacccgccg ggttttccgt accgtcaaca aagccgctca ggtcacgctc
    23461 ttcaacccaa cggaagccgt ggatctcttc tttcacttca atgcagtcac cgaaggcttc
    23521 catcgccgcc tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa
    23581 cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc
    23641 ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg ttaccaaagg caaccaccgc
    23701 accaagatgc gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt
    23761 actggccgca cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc
    23821 gcggcaatgt tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa
    23881 taatgccacg caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcggcggcg
    23941 gtttgttcag acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg
    24001 gtatcgtccg aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta
    24061 atatgttgac tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt
    24121 tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg
    24181 gtgtcagcag gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg
    24241 ttaatcacca tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg
    24301 ttcgcggttt tcattccgct gcgcaggcga taatcgccat caagcgcatc ggcaatggct
    24361 tgttcttgca gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg
    24421 gacgacccaa accagttcca cgggttagcg gccgaccagt taactgacga catcgtggaa
    24481 cagccggtca gcatcaacgg catagcgcat aacattaaac gcagcgattt catgtcactt
    24541 cctttggtta ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct
    24601 ttttcttcat taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca
    24661 atatccgcca ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc
    24721 cacggttgcc agcaaagtaa aaccacctgc gccaacagca acagaaaata gagtacacgc
    24781 caggtgcgag gaaacgtagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg
    24841 ccaggaatca gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca
    24901 gtaccctgct cgcgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc
    24961 aatagcacaa gccagaagag caagggtaaa cgcaggcgac cgtgcgcatc ataatggaca
    25021 ggatgaaact cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc
    25081 gtgttcatat cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt
    25141 gatctgaatt ttcgggcagc cacgagcaat cagctttttc tccagccgat taagcaacgc
    25201 attggcaatc ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg
    25261 atgcccgtca taaccgccca tcaccgttcc gaccacttca ccgtttacct cagcgaccaa
    25321 aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg
    25381 acgcagcaag tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa
    25441 tacgcgtatc tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg
    25501 aacggtcgaa ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt
    25561 tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat caaaccgtcg taacggatta
    25621 cgcgatacga tataacatct ggaactttat tattacaact caggccgtat gagcactttt
    25681 aaaccactaa aaacactcac ttcgcgccgc caggtgctga aagccggttt ggctgccctg
    25741 acgttgtcag gaatgtcgca agccatcgcc aaagacgaac ttttaaaaac cagcaacgga
    25801 cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggt
    25861 cacggcggaa ttgataccgg agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg
    25921 ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta
    25981 acgcgttctg gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat
    26041 ggcgcagatc tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt
    26101 gcttcggtat ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct
    26161 gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta
    26221 ttgcaacaag tgctgtttga tctggtgcaa acagatacca ttaaaaatag tctgacgctc
    26281 ggctcgcata ttctgaagaa gattaagccg gtgcataaac tgcacagccg caacaccgaa
    26341 caagcggcat ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg
    26401 tttatcacca acccggaaga agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc
    26461 gccacagcga ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca
    26521 cattcgaaaa agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc
    26581 aaccttcagg atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa
    26641 gatagttggc agcgcgaagc tggcggcggc gggcgtagtc gggtgttgcg taatggtggt
    26701 gttttcgaac aggcaggcgt caacttttct cacgtccacg gcgaggcgat gcctgcttcc
    26761 gccaccgctc atcgcccgga gcttgccggg cgcagtttcg aggcgatggg cgtttcactg
    26821 gtagtgcatc cgcataaccc gtatgttccc accagccacg cgaatgtgcg tttttttatt
    26881 gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg gcggcttcga tttaacccct
    26941 ttctatggtt ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgcctg
    27001 ccatttggtg aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc
    27061 aaacatcgca acgaacagcg cggtattggc gggctgttct ttgatgatct gaacacgcca
    27121 gatttcgacc actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat
    27181 ttaccaattg tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag
    27241 ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cactctgttt
    27301 ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc
    27361 tgggagtatg attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt
    27421 aaggtcaggg attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc
    27481 aacgacggct tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag
    27541 aatccccact gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt
    27601 tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa
    27661 atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat gcagttgatt acacaagtcc
    27721 agcaccgtca ccggctcaga catattttcc agcacatatt cacgggcgcg ggaaagtaat
    27781 ctacggtaac tctgatgact gatgctttcc gccgtcacca ttggttgcgc gtcttccagc
    27841 atcgccccca tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg
    27901 agattttccg ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg
    27961 gctttatgct gctctttcac ttccagcgcc gactggctac gcagcatatg taatacccga
    28021 tccgggttat gcaaaaagtt agcctgccgg gtgatgacat cttcagaaag caccacgccc
    28081 aggatcgtgt aatcatccgg cgtgctcagt tcaaattcag tgccaccagg gcgggtggcg
    28141 atttccgcgc ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga
    28201 atgccaaacc agaacgagtt cggccagacc aggcacgact gacgcagcgc cagaccggtg
    28261 tattcacgaa aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg
    28321 cccggatgca gctgatcgta aatctgctgc caggcggtaa tcgttaaagc atgttcatag
    28381 acatccgttg tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg
    28441 ttttcgggta agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg
    28501 atgttaatgc cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga
    28561 cgataacgac tactgcgttt acgtaacgtc ccggcagaaa agagctgttc gagcgcattt
    28621 ctggcttttt ccagcggcca gccaaagtgg gcagccactt ctcctgccgt cattccctga
    28681 cgtactgatg ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg
    28741 tcgggttccc ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg
    28801 tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac gcccggtacg catcgcggca
    28861 gcacaacctg cgtccagcgc cgcacgacac gccgccagat cgccttccac caccagcgtt
    28921 aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg
    28981 gcatccgctg catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg
    29041 atcatcggca acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca
    29101 tcggtaaagg cgttacaggc tgctttacac gcggcctggc taccggttaa aaatgctgcc
    29161 gagtagttgg tttcagacgg tggcgggaca taggtcacca gctgtacgtc ggcagatttc
    29221 aacgctgcat caatgccata ggtcgcttcc agcggcggtg ccaccagata tgccatcgga
    29281 tcgccaagcg tgatcccggc ggttgatgag agataagaac cggtacgcga aaccacatgc
    29341 gccaggaagg cggtgttttc tgcgtcattg gcccactgga atgctgcacc attttcaata
    29401 tgtgcaacca tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata
    29461 atcagcactt caccggcagt cggtgacggg ccgtgtgcag cgcccgcata cagcgagcga
    29521 ccatatacca cttccaccat cgcctgcttg gtcgcttcat cagcggcaat gtaggtgacg
    29581 tcatcggaat cagcagaaat cagcccgagg ctacgaatat gcggcggcaa ttttagttca
    29641 cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc
    29701 aaatctaaag ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc
    29761 tccagcatac gtttcgccaa atccacaatg acggctgcgg cttcaaccgg cggcgtgccg
    29821 ccctggtgaa tgttagagat acaggtgcga tcggcctcaa cggtggtcgc catacgcggc
    29881 gagtaaacgg cgtagcagga gaggctttct gactgcccta accccggacg ttcccccacc
    29941 agcaggatca ccactttcgc gccgaggatc tcaccgatct gatcttcaat cttcacgcga
    30001 ccataacgaa caaagaatgg cgtgccgact ttcagcccgg cctgtttcag gcccgccatc
    30061 agcggcggca ggatctcttc gtagttcacg gtgatggcat cagttgacag gccatcagaa
    30121 ataacgacct gtacgtccgg attagcaaca cactgtgctt tcagcgcttc aacagcttct
    30181 gcacacaggc ggcggcccat atccgggcga gtcaggtaga gatttttgtc gctaatttca
    30241 gagcgtactt ccagcaagcc ctgcgctttc acccactctt ccggcacttc tttcagtacg
    30301 gtatctttcg aacgagagtg atcggccagg aaacgcagca gcgcctgggt acgtagacgc
    30361 ggaccggcac gaccggtaca aacgcgggcc acggtgctgc ggcgcagttc tgttaatacg
    30421 tctgcgcgat gcgggttttc aacaccaatc cacgcttttg cttcagcgga acctaaatcc
    30481 agcgcacagc tttccgaggt caccggtgcc gcacagttgg tggtggcgca ctttgcttct
    30541 gacggcgcgg gggccgtttg tcccattgac gccatcacgc tgcgtacaat ttcttcaatc
    30601 tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg
    30661 cccgtttggt caggcgaccg tttgccataa tgcccatgct ttccagccag cgttcaaact
    30721 ctggtgacgg gcgcaggttg agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg
    30781 tctggtagtt gagcatgatg tcatcaccca gcggcatccc catgatgtag ttgcagcctg
    30841 cggtggcgag caggatcatc aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg
    30901 tgtagcagca gtcacagccc atagagatgc cgctcagctt gcccataaag tgatcttcta
    30961 agccagcacg gataatctgg cggtcgttgt agagatactc cggcccaata aagccgacca
    31021 cggtgttgac gataaacgga tcgtaatgac gcgccagccc atagttacgt gcttccatcg
    31081 tcacctggtc agcgccgaag ttagcgccag cggatagcgc agagccttgt ccggtttcga
    31141 agtagaggca gttttccccg gcgatacggt tgaactctgc acccactgcg cgcgcttcgt
    31201 cgagcatcgc cagttccacg ccaaactctt tcagcccttt ttcgctgcca cagatactct
    31261 ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg
    31321 ccagtacgca gccctgagtt gggatgttga atttgtcgat cacgccgtaa atggtgtcca
    31381 acacgcggct taagttttcc acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc
    31441 ccaccccgaa ggaaagccct tcgtagattt gcgcggcgat actttgcacg tcgtcacggg
    31501 tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat accaatggtg gtattggcct
    31561 ttttgattac cggcattttc ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg
    31621 ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc
    31681 tggtttcatc gctcagcaca tactcacgca gttcgctgat gctccagttt ttaatctggt
    31741 tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg
    31801 caatcaccgg attattgcgg atgtccgcta cggtcatttc cgacaacacc tgctttgccg
    31861 ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag cacatccccc gaacgcagtt
    31921 cgttggcttt agccagcacc tcttttacat ccttaaactg atatacattg ccgaacaatg
    31981 tggtctttag tttcataagt cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac
    32041 cggcacaacc gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac
    32101 aatcacttcg tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acaacatgcc
    32161 cagagcttta ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg
    32221 cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag
    32281 cgaagcggga agcgccagca cgtacgcgtc agttttggga tcaagatcca gctgaagcag
    32341 cgcctgttgc caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt
    32401 gcgcagcggc agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg
    32461 tgcacccgcg ccaatcaccg tggcgcgtac ggtttgcgcc ggaaactgca cattcatctc
    32521 acgcaggcgc ggatggtcat gcagcgccgt tgccagaagt gggccaatat cggcaaaaca
    32581 gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg cctccagaaa gcgtaatgat
    32641 ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc aatgcctgcg cgagcggcga
    32701 aagcgttccg tcaatcactt cgacaatcag cgccgccatc cgccgggtaa cctgcaccag
    32761 ctgcgcgccg gtcagcgaac gggcgtcagt gcctgcaccg aagcactcat ccacaatcat
    32821 ctgccccggt ttatgagcgt aaaccacgcg cccctggctg tcggtttcca gcaggcgacc
    32881 accgacgttg agacaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc
    32941 ggtgccaccg ccgatgtcga tattcagtac ccgacacagc cgttgttcag aaagggtttg
    33001 cgccccagct ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcaacgac
    33061 aaaatccccc agcgattgag agagcgccat caccgccggg cgagcattgc gggttttcgc
    33121 gctttcaccg gtgatgatga tggcaccaga atcaacgctt tccggcgcaa tacccgcagc
    33181 ctgatattgc tcgagtatta aggttttcag ttccgcttct tttaaaccgc cctgtttatc
    33241 gacaggggta aagaacaccg gactttgcca gctaatttcg cgtttaatga attcgtagcg
    33301 cggcacctgc gacaccgccg cacggttaac cagctccagg tgggagaaaa tcacctgggt
    33361 ggtggtggtg ccgatatcga taccgacgct caatagctgg cgagtgttca cgattgtgcc
    33421 tccgcttcgg ttttagtcgc ggtcgcgtct tcttttggca ccagcatcat cgccacgcca
    33481 atcgccgtta cgccgccgat caacttgccg acaatcatcg ggaagatcat ggcgttcatg
    33541 ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg
    33601 cagttgatga ctttgccgcg ggtatccatc tgcttcatca tgccgaacat cgggatgttg
    33661 ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt tcatattcag tactttaccg
    33721 acgctcatca gcggtttttc aaaccagcga gtcagcagca gcaccattgg atacgcccct
    33781 aacagcacgc aggagataga accgataact tcaatggcgc gcatcacttc accgggttta
    33841 tcgccagggg ccataaagat aggatccaga ccggggatca gttcccagcc aagcaggaat
    33901 ttcactaccg cagcggcaag accgagggtg atcaatgcaa cgaggaattt ggcgaagatc
    33961 tggaagccgt tgatcatttt ttccgggatg aatttcagcc ccagcgccac cagaatcgcg
    34021 acaatgatca ccgggatcat gttcatcagg atcagggcga aagtgaattc caccggctga
    34081 ccgttgatct gcacaccgga gtacatagca accagaccac cagcaataca gccaatcgga
    34141 atggtcacaa tgcccgccag cacgccgagc gccagataac gacggtcaga aggttcgata
    34201 atgccgagcg ccaccggaat ggaaaacaca atcgttggcc ccatcatcga cccaagaatt
    34261 aacccagagt atagccacgc tgctacgtcg ccgcccgcca gctctttggc gaggaagaaa
    34321 ccgcccatat cgcacgccag cagcgttccg gcgaacattg acgggtttgc gccgagcatt
    34381 tcgtaaaccg ggataattac cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc
    34441 ataccgacca tcgccagacc cagagcgccc attgccataa agccttcttc gaactgaccg
    34501 cctgatcctt cgatactttt accgaactta ccgaggaaac gagcagaacc gccgaactgc
    34561 gacaggatcc tgtctacggc agctatcagc ataaagaaca tcatgatgta catgatgatt
    34621 tcgttaattc ccatcgcctt tactccctgt tagttgttat ttattggcgg atgcggcgta
    34681 aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg atcagacgcg gcaagcgtcg
    34741 catccggcat ttattgcgcc gctgcgtaca ggccgacaat ctgctccagg ctggcggtac
    34801 gcgggttgct gcgcagacaa atatcttcct gcgcggcctg cgcccatgca ccgtaatgcg
    34861 cagatgtcgc gccaacatcg cccagtcgtt taccaatccc aacttccgca atcagctcac
    34921 ttaccgcgtt aatagcgtca cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct
    34981 gactaaaccg ttcgcgacaa accatccggt taaattccat caccgttggc agcaacatgg
    35041 cgttcgccag accgtgtgga atatgcagcg ccgcccctgg ctgatgcgcc atcgcgtggc
    35101 acaacccaag ccccgcactg gaaaacgcca ttcccgccat acatgaagcc agtaacatgc
    35161 tctcgcgcgc ggcaaggtca tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca
    35221 tcgcaatggc accaatcgcc agactgtcgg taaacggtgt ggcgttcagc gcgctgtacg
    35281 cttcaatggc atgggttaac gcatcaatgc cagtcatcgc cgtgacatgc gacggcacac
    35341 cttcggtcag cgcggcgtcg aggatcgcca catccggcat cagcgaggca tgggctaaca
    35401 cctgcttgcg cccgctcacc gtgtcgataa tcaccgttac gttggtggtt tcagagccgg
    35461 ttccggcggt agttggaatg gcaatcaacg gcaggcgcgg ttgcagaacg ctggtttctg
    35521 acatttccgc cagcgtgcta tccgggttcg tcaccagcaa cgccacggct ttcgccgcat
    35581 ccagcaccga gccgccgcca aatgcgatca ccccatcaca gcctgactca cgcaactgcg
    35641 ccacggctgc acacacgtcg gtgatgcacg gttcgcccac cggacatggc cagagcgtca
    35701 tggcgatgcc tttaacagcc aggctgcgcg tcagcccggc ggtcatccct gcctgatgca
    35761 aaaagctgtc tgccatcacg aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc
    35821 aactgctcac cgagcccgga ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc
    35881 gttgcagatt cagggtatcg aacgcctgaa agagcgcggt ctgcaattca ttttgcatat
    35941 agcccctccg ctttctctcg cccactactg gcgatcgcca gcggggtcat aaacaaactg
    36001 tgctgcggta aatgcacttg taacgccggg aattgtttgc gaaacagttc cgccacgccc
    36061 ggttgcatac aggagccgcc cgccagccat aaatcagtaa tgccctgccc ttcaatatgc
    36121 cgggcgacaa tgtccgccat tttttcgtac accggtttca ccgcaggcca aatctcttca
    36181 ccgtgaccgc gcttgtactg ctccgcctct tccagcgaaa tacggcgatt tccggcgaga
    36241 gtcagagaga tgtgatgtcc gccggtggct tcatccgccg agtacgtcac tttgcccttt
    36301 ttcacgatgg caatgccggt agtgccgccg ccgatatcca ctacaccggc gttgtccagt
    36361 tgcagcagat ccgcgactgc ggttggctca tccagcacat ggctcacttc cagcccggca
    36421 gattccagca cgttgatgga aatacgcggg tccgtacctg gaggaaatga agtcgccgca
    36481 tggctaaaac ggcgaccgaa ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg
    36541 acagcgccga agaaatccca gacgatgccg tcgcggacca catcggccca gtcgaggcat
    36601 accgccaccg gctgaccgtc gcggtcgaca accatcgaca ccacatcgca ggtgcccaga
    36661 tccaccccca gccacagcgg agattccgtc gcggcaggcg tctggttaca cagcgttgcc
    36721 gccgtttgca gacgtggggt gagccattgt tcgtcgtgcg ccatcggtta ctccttaaac
    36781 aatgcgaaac gcatcgacta atacacagcg acgcagacgg acaaacgtcc gcgcgctggt
    36841 taccccttca ccggttggcg tggtgatggt catggtggtc cagccttccc cgcccagccc
    36901 cagcccggca atgcacggtc cgttcttaac gaaaatgctg gtatcaatag cattcgccat
    36961 ctggttcatg ttttcgatgt tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc
    37021 cagtttcacc gccagcgcaa tggcatccgc cacgttggcg acgcgcacga cgggcagcac
    37081 cggcatcatc agttcggtca cggcaaacgg atgttctgca gtggtttcca caaacagcaa
    37141 acgcgtttct tgcggcacgt tcaggccgat tgccgccgcg attttcgccg catcgcgacc
    37201 aacccagtca cggctgacgg tgccttttcc gcgctcgtcg atatttttca gcaacaccgg
    37261 ttgcagctgc tgcgcctgtt ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg
    37321 catcagttca tcggctacgc tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat
    37381 gttgttatcg aaagaagcgc ctttgacgat ggactgagcg gcacgggcga ggtcggcggt
    37441 ttcatccacc actaccggcg ggttgccagc gcctgcggca atcagacgtt tattggtgtg
    37501 tttacgcgcc gcttctacta ccgcttcgcc gccggtcacc accagcaggc cgatacccgg
    37561 aaacttgaac aagcgttgcg cggtttcgat atccggattt gccacagtaa ccagtaagtt
    37621 ttccggccca cctgcggcaa caatcgcctg gttgagcagc gtaatcgccc gctgggagac
    37681 ttttttcgcc gccggatgcg gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat
    37741 ggcgttatta attacggttg ccgccgggtt agtggacgga gttaccgaag ccaccacgcc
    37801 ccagggcgcg ttttcaatta aagtcaggcc gttgtcgccg gtcagcacct gcggagagag
    37861 acactcaacg cctggcgtgc cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac
    37921 gcggcccatg ccggtttcac tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc
    37981 tgcctcacga atggcagcaa tggctaactg gcgcattgcc acgcttttta acccttgctg
    38041 ggcgactttg gctgccgcaa cggcgtcatc cagggacgcg aaaacgccca tctcatgaac
    38101 ggcggcggac ggcgtgtcac tgctttgcat tttcagcagt accgctttca ccacctgttc
    38161 aatatcctgt tgattcatga tgttctgcct tatttgtgga aaattacctg accgccagac
    38221 accacctcat cgacaatgcc aatcacgcac agatcgaccg gtgacgtttc gcttttatgc
    38281 gcctggcggg cggaactgcc actcaccagt aacacccact ccccggttcc cgcgccaata
    38341 ttgtcgatgg cgacggcgca ttgcccgtca ggattacctt gtggatcaat catttccacc
    38401 atcagcaatt tgtcatgcgc cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg
    38461 acgactgcca gtttcatacc cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc
    38521 tccgcagaag gtaaaatgaa aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg
    38581 tgattacagg ttgctgctat cgcctttcag gccgatcggg aagacttctt ccaggtcgcc
    38641 gtgcggacgc gggataacgt gtacagatac cagctcgccg atacgctgtg ccgctgcggc
    38701 acctgcatcg gtcgcggctt tacaggctgc aacatcacca cgaaccatgg cagtacacag
    38761 gccgccgcca atctgcttaa caccaaccag cttgacgcgc gcagctttca ccatcgcgtc
    38821 agaagcctca atcagtgcaa ccaggccccg ggtttcgatc attcctaatg cttccattgt
    38881 gctttcctct ttatcagggt ccagaacggg accgttcatt caaccagtgt ttgtaaactg
    38941 ctttcgcggt tcacttttgt ctgacgcggc acagctgcca ccagcgccag ctcgataatt
    39001 tcctgcacgc tacaaccacg agagagatcg tgcatcggcg cggcaagtcc ttgtatcagt
    39061 ggcccgacgg cacgatatcc gccgagtcgt tgtgcgattt tgtaaccaat atttccggct
    39121 tccagcgacg gaaaaaccat cacattggcc ttgccctgta gcgggctggc aggcgctttt
    39181 tgcgccgcca cttccggcac gaaggcggcg tcaaactgta actcgccatc aaccaccagc
    39241 tttggtgcgc gctcacggac gatttctgtc gcctgctgga cgttggcaac gcaggggtga
    39301 cgggcgctac cgttactgga aaacgacagc atcgccacgc gcggctcttc tccggtgatg
    39361 gcgcgccagg tttcggcact ggcaagcgcg atatccgcca gctgcgccgc cgtcggctgt
    39421 ggcaccacgc tgcaatcggc aaagcccaac gccgggccgc tgtactgtgg caacatcagg
    39481 aaaatggaag agagcgtttt acagcctggc tgcaagccga taatgcgtaa tccggcacgc
    39541 agcacatttg ccgtggaaga gaggttgccc gcgatacaga catccgcttt accggcgctg
    39601 accattgctg cggcgaacat cagagggtcg gtaagttttt ccagcgcatc cggcggcgtt
    39661 ttttcgcccg cgcgggccag ccagcgatga gcaaattctt cccgcattgc gaggttgcca
    39721 tgcggatcaa tcacctgtag cccgtccatc gccacgccgt gactgagcgc aaactgacga
    39781 agttcaaacg gattggcgac cagaatggac gttgccagac cttgttgatg taaatattgc
    39841 gcggctttca gcacacgttg gtctaacgca tccggaaaaa ccactctggc gggcgctcgc
    39901 aacgccagtt cacgacaacg ttcaataatc attgcgtttc ccccagtcgt tgttggatct
    39961 gcttcaccgt tagcggctgc ttcgttacgc tcaggatcat catcacgtag accgtgctcg
    40021 aaaggcggtt cagcgcctgc aaaatgtccg ggcgtaatac ttcaaagctg cgggtgataa
    40081 acacctgcgc cgctacggtt tcggtttccc gcactttggt acgtagcaga ttgagcaacg
    40141 ccgcatcgcg cccgtggctg gcttccggca ccagatgatc gtggtcgaga tagcgcagcg
    40201 gctgatgaga aagccgatgc agatcttcgt cagaaagccc gacaatcgcc tggcagccca
    40261 gcggttcccc cagcgcatcg gcgcgcataa tgttgccaag acgcgaacgg atatccgcta
    40321 gccacggctg ccacggttcc gccagttcaa tttgcagcca cactgccagc gcaatggtgc
    40381 tgtcgagaac cgcgcgaaaa cccagacgcg gatcgctttt ggcgaccatt ttttccgccg
    40441 acaggtgagt cagcgtatcc ggcttttttg ccaccggctg gcgacacagt tcgcagcacg
    40501 cctgcggatg ttcatcgcta ctggtcagcc catgaacggg ctgcggctgc tgctgttcat
    40561 cgtcaacaaa caggcggccc tgctcgtcaa taaacttgat gcgcagatgg cggctttcca
    40621 gcaactcccg ggcagagggc gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt
    40681 cgctgagcgt atggttcgct cttagccatg cttcggtgat gaaatctttc atagggattg
    40741 ccagttagcc ggccaggcga catacaggaa tttcacgctg gatgtcgtac caaattcgat
    40801 gctggagcct ttcgggataa acatcacgtc gcctgctttg gcgatcatgg tttggccttc
    40861 gtggcgaaca tgcagctccc cttccagcac catgtcgatt tcgtcgtagt tcagggtcca
    40921 cgggaagaat gcgttctccc actgcataaa accggcggcc atgctgctgc cgtcgtctcc
    40981 ggtcacaaga tcagtcaagc ccacgcaatg cggctctgcg ccgtcgaaac gaccaaactt
    41041 gacgctactg ccatcaatca ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg
    41101 ctgcattgcg ccctgctcca gcgactgttt ttccttcatc actttttcca tcagctgtgc
    41161 gaccaggctt tcggtaaact ggccttccgg cagctgggca atgatggttt cgcggatgcg
    41221 ctggctttcg gttttgtcgg ctggcacgct ggcgggcacg gacgccgtta ccgggatcga
    41281 ttcatcacat tcggtgatgg taaaacccag tagatccgcc acttcacggg cttccggggt
    41341 aataatgctg gcgcgcagaa ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg
    41401 aatatcattc gctgtgatga gttttttcac ctgcttcctc ctgtttggtg agagatgcca
    41461 gataatccac cagttgctgc acgctttgcg ggtcgtggct gtttagctcg aaaatcggct
    41521 cttcgaaacc cgtctcaagc aataactttc gcgttgcggc gacgtcagca tctggcatgt
    41581 ccgttttact gatgacggcg atttgccgtt tgctgacgcc aatatccagc aacccggcag
    41641 gcaaccgact ttccgggtca tttgcgccat ggacatagat cagcatgtct acatcctgta
    41701 gcgtggtaat taaggcgtga taccagcggg gatggctaaa atattcaccc ggcgtatcaa
    41761 tatcgccctt atcattaaat tccacggcct gtgtttttct ggcgagggta taattcccct
    41821 gcaacgcatt aaataacgtt gtttttcctg ccccgacgct acccacaaac gcaattcgtt
    41881 tcatgaatgc ctcgtattaa cttttggtca tttcacaaag cgtataattt aataagcgac
    41941 ctaaaccgct gacggtttgt gataacgctt cttctactgc gcccaccgag ccataaatca
    42001 ccaatgcacc gctgaagcgg tcgagaaaac cgatatgcac atcagcggct ttcagcgcta
    42061 aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt cataatgcca attgcgcccg
    42121 catccggaac gccgattttc ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca
    42181 gtgtgacctg tttgccgggc acaaattcct gaatgatgcg ttctttatcc atgagtcgcc
    42241 gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc agagtaaata acaaaattcg
    42301 gcaagcgcgt ttaaaaggtg aggtagatca ctaagaatgg agagatattc gctgtggtgc
    42361 ataaactttc atatgcaacg catgaatata aataaaataa tatcgagata ttatatagac
    42421 tgcttttttt attattcgca aactcagtaa taagctttac taatatattg cagccaaacc
    42481 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa agaattacag cggttgggtt
    42541 tgcgcttcaa ccacggccag cgccaccatg ttgacgatac gacgcaccga tgcgatcggc
    42601 gttaacacgt gaaccggttt cgccacgccc atcagcaccg gaccgacagt cacaccttcc
    42661 gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc
    42721 agaatattgg cagaaccttt caaagggctg tccggcatac ggtcgttgcg aatcgcttcc
    42781 accagcgctg catcgccgtg catttcacca tcaatcatca gatctggcgc acgatccctg
    42841 accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg
    42901 gagtgcgaca acagagcaac gcgcggctca ataccaaaac gacggacagt ttctgccgcc
    42961 atcaaggtga tctccgccag ctcttctgcg tctggttcgt cattgacgta ggtatcggca
    43021 ataaaggtgt taccactcgg cagcagcagc gcgttcattg cacctgcggt gtgaacgcca
    43081 tcgcgataac caaagacatt tttcaccacg ctaaaatgtt catgataatc gcccaccgta
    43141 ccgcagatca ttgcatcggc ttccccacgc tgaaccatga tcgcgccgat cactgtcggg
    43201 ttactgatca gcgcccgctg cgcctgttcc tgagtgacac cgcgacgctt catgatctgg
    43261 aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa
    43321 tcaacgcccg ctttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga
    43381 cgaccgataa ggatcggttt cgccagtccc agcgttacca gttcctgagt ggcatgcaga
    43441 acgcgcgcct cttccccttc cggcagaact acgcgcttcg gcgctttgcg agcctgggag
    43501 aaaatcggct tcataaacag gttggttttg taaacgaact cagtcagctt gtcgatgtag
    43561 acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact ccatcgccgc tttcgccacc
    43621 gcaggagcga tcttaacgat caagcgcgga tcaaacggtt ttggaatgat gtattccgga
    43681 ccaaagctca gatcctgatc gccatacgct gaagccacca cttcgctctg ttcggcgtgg
    43741 gccagttctg caatcgcgcg taccgccgcc agtttcatct cttcgttgat ggcggttgcg
    43801 ccaacgtcca gcgcgccacg gaagatgaac gggaagcaaa ggacgttgtt gacctggttt
    43861 ggatagtcag aacgaccggt gcagatgatg gcatccggac gcacttcttt cgccagcggc
    43921 ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgcacg agccattttc
    43981 ttcaccattt cctgggtcag cactttcggg ccggaacatc cgaggaaaat atccgcgcct
    44041 tcaatcacat catcgagggt acgtttgccg tcatccacca ccgcatatgc ggctttggtt
    44101 tccgccatgt ttggctcacg gccctgatag ataacgcctt ttgaatcgca aaccacgatg
    44161 ttatgttttt gcagacccag cgctaccagc aggttcatac aggcgattgc tgcggcaccc
    44221 gcgccggaaa ccaccatccg cacgtcggag atgtttttct ccaccacgcg caagccgttg
    44281 aggatggcgg cagtgctgat gattgccgtg ccgtgctgat cgtcgtggaa taccggaata
    44341 ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg
    44401 aggttgattc cgccgaaggt cggttcgagc gcagcgacaa cttcaataaa tttgtccggg
    44461 tcgagttcat caacttcaat gtcaaataca tcaatcccgg cgaatttctt aaacagaacg
    44521 cccttgcctt ccatcaccgg tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc
    44581 gccgtaccgt tagagatcac cgccaccagg ttaccgcgcg cggtatattt gtaggctttt
    44641 aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc
    44701 agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga
    44761 aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca
    44821 cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt
    44881 attaccctgc cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg
    44941 ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt
    45001 ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca
    45061 tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt
    45121 tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg
    45181 atgccaccac caatccttcg ctgttactca aggctgccgg attatcacaa tatgagcatt
    45241 taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg
    45301 tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg
    45361 gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga
    45421 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattttga
    45481 tcaaactggc ttcgacctgg gaaggaattc gcgcggccga agagctggaa aaagaaggta
    45541 ttaactgcaa cctgacgctg ctgttttctt ttgcccaggc gcgggcctgt gcagaagcgg
    45601 gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc
    45661 cgatggaccc gtatgtggtg gaagaagatc cgggcgttaa atcggtgcgc aatatctacg
    45721 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
    45781 aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg
    45841 agctgcagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct cagacgttcc
    45901 cacgcccagc tcccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg
    45961 cggtagaaaa actgtctgaa ggcattcgtc tgttcgctgt tgatcaacgc aaactggaag
    46021 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
    46081 gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc
    46141 ggcgcgccga tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat
    46201 aaccctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
    46261 tcgatgctgc tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg
    46321 aagaacttcc gtcagttgca ttcgaaaacc ccaggccacc cggagattgg ctatacggca
    46381 ggcgttgaaa ccaccaccgg cccgcttgga caaggtttgg cgaacgccgt cgggctggcg
    46441 atagcggaac gtacattggc ggcgcagttt aaccagccag accatgagat cgtcgatcac
    46501 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt
    46561 tcgctggcag gcacgctggg actgggcaag ctgattggtt tttacgatca caacggtatt
    46621 tccatcgacg gtgaaacaga aggctggttt accgacgata cggcaaaacg ttttgaagcc
    46681 tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg
    46741 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
    46801 ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc actgggggaa
    46861 gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
    46921 aaaaagattt atcacgcctg ggatgcccgt gaaaaaggcg aaaaagcgca gcagagctgg
    46981 aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttacccga
    47041 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag
    47101 ttacaggcaa atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct
    47161 tacgggccga tgctgcctga gttgctcggc ggttcggcgg atctggctcc cagcaacctg
    47221 accatctgga aaggttctgt ttcgctgaag gaagatccag cgggcaacta cattcactac
    47281 ggggtgcgtg aatttggcat gaccgctatc gccaacggca tcgcgcacca cggcggcttt
    47341 gtgccgtata ccgcgacgtt cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg
    47401 gcggcactga tgaaagcgcg gcagattatg gtttataccc acgactcaat tggcctgggc
    47461 gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat
    47521 ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt
    47581 gagcgccaca acggaccgac ggcactgatc ctctcaaggc agaatctggc ccaggtggaa
    47641 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc
    47701 ggtaagccag atattatttt gattgccacc ggttcagaga tggaaattac cctgcaagcg
    47761 gcagagaaat tagcaggaga aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat
    47821 attttcgacg cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct
    47881 cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg
    47941 gcaattgtcg ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc
    48001 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt
    48061 gcctgatggt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt cttatccggc
    48121 ctacagcgtc ttatccggcc tacatgtccc cgcaattttg tttaacgggt gatccacaac
    48181 gtgggccagg cgtctggccc atgccagtta tcgcaggtgg gttctgcggc ataacgcacc
    48241 aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg
    48301 cgccctttcg ctaaggtcac cagttcacgc gatttctcat caaatgttgc gttcatcagt
    48361 tccagttcat tcgtctcctg accgttgttg aacggcaaac gcaaccgaac cgggcgcgag
    48421 gctagtggct ttttacgcga cacaatccat gccaaatcaa tggtgttata ggcccctgcc
    48481 tcacagctaa tcatcatcag cgctttatca tcagtcagcg cagtgacatt cgcctcacga
    48541 cgtaacggat caagcgagca gcgcagacca ttcatccgcc agtttccata atccagcaaa
    48601 acgttgcgtt cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc gacctctttc
    48661 agcgcaggcg cgggcggtac gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt
    48721 tcactgccaa cgcgcttttg ctgagcatca ataaacaaca acgccgcttt taagccactc
    48781 agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc
    48841 aaaaacgcgg tgatggttgc cgtatcatcg gttactaata accatggtga aatccgccac
    48901 ttgtcaccac ttaacgccaa cggctcacca tctaacagca gccgtggcgc tatctctcct
    48961 tcagacgcat ccggcgactt caacccgccg cgctcaatac gtaaaacggc atcggtatgc
    49021 gccccggcgc tgcggctcag ggtcatcacc agtccattat gatcgcccgt attgcgcgcc
    49081 acgcagaagt tttggttatt acaggtgacc tgccagtcgg aaaacgcccg ttgcgctggt
    49141 gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag
    49201 cgcatggacg gtacgacccc agaagttcaa acaaacagca caagtcgtat cttcgttgcg
    49261 caggtagcgc ggctcaatca gatttgtcgg attgagccaa taataaacaa cgaaatatta
    49321 ttgttatttt tcagtccatt aaatgtgacg tttgcaaata gttaagcaat aacaccgtct
    49381 taccgtcacg tatctcgccg gttttgatca tctccagcgc ctggctgaac ggcagctcga
    49441 gcacttcaat atcttcatct tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt
    49501 attcggcgat aaaaaagtgg attagctcag tcacaccgcc aggcgacata tacagttcaa
    49561 ataatttgcg cacttcgccc acttcatagc ccgtctcttc aatcgcttct ttacgaatgc
    49621 acacttccgg ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt
    49681 cattgccatt aacccaggta gcgacacgga actgacgaat cagaaccacg gtctttttct
    49741 tcgcgttgta caggaggatc gtcgcaccat taccgcgatc gtaaacttca cgtttatggc
    49801 ggataacttc gccatctttg cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt
    49861 tatcggagag aattttgtct ttaatgaggg tgatttgttg cgtcataccg actccacagc
    49921 gcgaaatgaa caattatatt acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga
    49981 gatttgactg attttacccc cagccagtca ataatcccct gtgccgcatg acgtccttct
    50041 gccattgcgg taaccaccag atccgcacca cgcacggcgt cgccaccggc gaagattttc
    50101 ggattggtgg tctggtaacg gtactggctt tccacatccg cgatgatgcg gccccatttg
    50161 tctaccgtta caccgtgcga ctccagccac ggcatcccgt gtggattgaa gccaaacgcc
    50221 ataatcaccg catcggctgg catgacaaat tcactgccct caatcggtac aggtcgccga
    50281 cgcccctggg catccggctc gccaagacgc gtgcgcagga aacgaatccc gcagacgtga
    50341 ccttgttcat tcagctcaag cgccaccggc tggacgttaa attcgaagtt ggccccctct
    50401 tcgcgggcgt tcctcacctc tttcttcgag cctggcatgt tagcttcatc acgacgataa
    50461 gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac gcacacagtc catcgcggtg
    50521 tcgccgccgc ccagtaccac gacgttaagt ccggcggtat tgataaacgg ctcttccggt
    50581 agctcttcga gccccatcac ctgtttagtg ttggcaatga ggaacggcag cgcgtcataa
    50641 acgcccggcg catcttcatt gggtaaaccc gctttcatgg aacggtaagt gcctacgcca
    50701 acgaagaccg cgtcgtattg ttccaaaagc gaatccaaag agacatcttt acccacttca
    50761 caattgagtt cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg tgccagcagg
    50821 gatttatcca gtttgaaaga aggaatgccg aaagtgagca agccaccgat ttctggatgg
    50881 cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa catccgcaca ggccagccct
    50941 gccggacctg ccccgataat cgccacccgc ttgtccactt tggtgacatg acttaagtca
    51001 ggacgccaac ctttcgccaa cgcctgatct gaaatatagc gttcaatgtt gccgatagtt
    51061 accgcgccgt gctcatcgcg aatagtacag gcaccttcac acaaacggtc ttgcggacaa
    51121 acgcgtccgg taatttccgg cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg
    51181 atgtttccgg ctttcaccag ttcaatccac tgcggtatat ggttatgcag cgggcaggtc
    51241 cattcacaaa cgctgtgctc gccgcactta aggcagcgcg aggcttcccg ttgtgcctgg
    51301 tcggcgcgaa atggcagata aatttcatca aaaccggttt tgcgcgcttc aatcgccagt
    51361 ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt cgactttact cattaccggc
    51421 atttcttgcg ccgcggtact ggcatgccac ggttgatgtt cctgacgcgc ggtgcgcaag
    51481 cggcgggatt tcgccatgcc ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc
    51541 gggcaattct caacacaggc aggaccgttt tcgcgccccg cacaaaggtc gcatttatgc
    51601 gccgtggctt ttacttttcc tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaaa
    51661 ggacaggcca ccacgcagga tttacagcca atacactttt gctgattgac ctgaatgctg
    51721 tcatcaacgt ggctgattgc gccattaggg cagctacggg cgcagggcgc atcttcacaa
    51781 tggtgacagg tcactgcact acgttgctgt tgatgtttga taaccgtaat tcggggatga
    51841 aaatggtgtt ggctcaggac atgttgctca tcattgtgag ccatgacaca ggcgatttca
    51901 caagcatgac aacccagaca ttgctgactg ttggccataa taaaacgatt cataacgacc
    51961 ttcttttttg gttgtaaaaa ccttattctt tatatgagtg ttgttattac ccgacttaca
    52021 ggggaatcgg caatgttcat gtgcccagaa taagtaacta tttcgctata aactgtggca
    52081 gatcaaataa tcccatcact gactaaattg tgtttcagcg aactggaaca ttaatgattt
    52141 tttgtggaga agacgcgtgt gattgttaaa cgacccgtct cggccagtct ggcccgggcc
    52201 tttttttaca ttgtgctgct gtcgattctt tccacgggta tcgctctgct aactctggcg
    52261 agcagtttgc gcgacgctga ggctatcaat attgccggat cgctgcgtat gcagagttac
    52321 cgcctgggct acgacttgca aagtggcagt ccacaactca atgcacatcg ccagttgttt
    52381 cagcaggcac tgcattcacc ggtattaacc aatctcaacg tctggtatgt gccagaagca
    52441 gtaaaaactc gctatgcgca tctgaatgcc aactggctgg agatgaataa tcggctcagc
    52501 aagggcgatt tgccgtggta tcaggccaat attaataatt atgttaatca gatagacctg
    52561 ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc
    52621 ctggctggcg gtatcggtat tttcacgctg gtctttttta ctttgcgccg catacgccat
    52681 caggtggttg ccccgctgaa tcagctggtt accgccagtc agcgtattga acacggacag
    52741 ttcgactcgc cgccgctgga taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc
    52801 tttaaccaga tgtcgagcga gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa
    52861 gaaaagaccc gcgatctcca cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcc
    52921 caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt
    52981 cgcgacaatg aagcggctga atatctggag ttaaatgtcg gtgaaaactg gcggattagc
    53041 gaagggcaac caaacccgga attgccgatg cagattttac cggtgacaat gcaagagacg
    53101 gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac
    53161 agcgtttcgt cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag
    53221 cagttattgt tgatggaaga acgtgcaacc atcgcccgcg aattgcacga ctcgctggct
    53281 caggtacttt cttacttacg tatccagttg acgttactga agcgttcgat accggaagat
    53341 aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga tgcttatcgg
    53401 cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga tctcccctcc
    53461 gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgccaaact gaccctcgac
    53521 tgccgtctgc caaccctggc gctggatgcg caaatgcagg tgcatttgtt gcaaattatt
    53581 cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc
    53641 gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat tggcatcggt
    53701 gaaccgaaag agcccgaagg tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg
    53761 ctgggtggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc
    53821 tttcgctccg cggagggtga ggaaagtcag ctgatgtaat gcctcctact gaccaaagaa
    53881 tacttgcact taaggttcag tataaaaggg catgataatt tacattaact cctttttttc
    53941 tccacgattg gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacgatacgc
    54001 agaaacacga ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg
    54061 ggtgctggca atcctgttgt gtctgacagg taccctggcg attttctcat tgcccgttga
    54121 acaatacccc gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc
    54181 ggcccagacg ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga
    54241 taatctcatg tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag
    54301 ttttaaagca ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc
    54361 agccatgcga aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg
    54421 cgataccaat attctgacca ttgctttcgt ctctaccgat ggttcgatgg ataaacagga
    54481 tattgccgat tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg
    54541 ggatatcgat gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact
    54601 caacagtttc cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca
    54661 gattgcggtt gggcaacttg gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac
    54721 cattaacgcc cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt
    54781 gaatcaagac ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga
    54841 gaaatacgat tatcttagcc gcttcaatgg taagccagcc tccgggctgg gggtaaaact
    54901 ggcctccggc gcaaacgaaa tggcgacagc ggagctggta ctcaatcgtc tcgacgagct
    54961 ggcgcagtac ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt
    55021 taaagcctcc attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct
    55081 cgttatgtat ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc
    55141 ggtggtgttg atgggaacct tctccgtact ttacgccttt ggttacagcg tcaacacctt
    55201 aaccatgttc gcgatggtgc tggcgatcgg tctgctggtg gatgacgcca tcgtggtggt
    55261 ggaaaacgtc gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa
    55321 atcgatgggg cagatccagg gggcactggt cgggattgcg atggttcttt cggcggtatt
    55381 tgtaccaatg gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac
    55441 cattgttgcg gcgatggtgc tgtcagtact ggtagcgatg atcctcactc cggctctgtg
    55501 tgccacacta cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc
    55561 ctggtttaac cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat
    55621 tctccaccgt agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt
    55681 cctgttcctg cgtttgccga cgtcgttctt accgctggaa gaccgtggca tgtttactac
    55741 ctcggtacaa ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat
    55801 cgagaaatat tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccactgttgg
    55861 ttctggcccc gggggtaacg ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg
    55921 gagcgaacgc gacagtaaga ccggcacctc gtttgccatt atcgagcgtg caacaaaggc
    55981 gtttaacaag attaaagaag ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct
    56041 tggtagttct gcgggttttg atatggagtt gcaggaccac gctggagcag gtcacgatgc
    56101 gctgatggca gcacgtaacc agttgctggc gctggcggcg gaaaacccgg agctaacccg
    56161 tgtgcgccat aacggcctcg acgacagtcc gcagttgcag attgatatcg accagcgtaa
    56221 agctcaggcg ctgggcgttg atatcgacga tattaacgac acactgcaaa ccgcctgggg
    56281 ttcgagctat gttaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc
    56341 agctgcgccg tatcgcatgc tgccagatga cattaatctc tggtatgtcc gaaataaaga
    56401 tggcggcatg gtgcccttct ctgctttcgc gacctcacgc tgggagacag gctcgccgcg
    56461 tctggaacgc tataacggtt attctgcggt tgagattgtt ggggaagccg caccgggggt
    56521 cagtaccggt acggcgatgg atattatgga atcgttagtg aagcagttgc caaacggctt
    56581 tggtctggag tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc
    56641 gctgtacgcc atttccttgc tggtagtatt cctgtgtctg gcggcattgt atgaaagctg
    56701 gtcggtaccg ttctcggtaa tgctggtcgt gccgctgggg gttatcggcg cgctgctggc
    56761 aacctggatg cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat
    56821 tggtttatcg gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa
    56881 aggccacgac ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct
    56941 gatgacctcg ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg
    57001 ttccggtggt cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagctactat
    57061 tctggctatt tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa
    57121 gccgcgcccg gaataagcaa taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt
    57181 tccgattaag aacctgctca gcgggttctt gctgtttata ctttgtctcg ggaatt
//
LOCUS       DAFAVF010000013        56318 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFAVF010000013 DAFAVF010000000
VERSION     DAFAVF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.013"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(197..283)
                     /locus_tag="LGK42_000757"
     tRNA            complement(197..283)
                     /locus_tag="LGK42_000757"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Leu,seq:gag)
     gene            complement(298..630)
                     /gene="secG"
                     /locus_tag="LGK42_000758"
     CDS             complement(298..630)
                     /gene="secG"
                     /locus_tag="LGK42_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK2657037.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(858..2195)
                     /gene="glmM"
                     /locus_tag="LGK42_000759"
     CDS             complement(858..2195)
                     /gene="glmM"
                     /locus_tag="LGK42_000759"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK2657038.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2188..3036)
                     /gene="folP"
                     /locus_tag="LGK42_000760"
     CDS             complement(2188..3036)
                     /gene="folP"
                     /locus_tag="LGK42_000760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBK2657039.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3126..5060)
                     /gene="ftsH"
                     /locus_tag="LGK42_000761"
     CDS             complement(3126..5060)
                     /gene="ftsH"
                     /locus_tag="LGK42_000761"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK2657040.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5160..5789)
                     /gene="rlmE"
                     /locus_tag="LGK42_000762"
     CDS             complement(5160..5789)
                     /gene="rlmE"
                     /locus_tag="LGK42_000762"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBK2657041.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            5915..6208
                     /gene="yhbY"
                     /locus_tag="LGK42_000763"
     CDS             5915..6208
                     /gene="yhbY"
                     /locus_tag="LGK42_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK2657042.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6364..6840)
                     /gene="greA"
                     /locus_tag="LGK42_000764"
     CDS             complement(6364..6840)
                     /gene="greA"
                     /locus_tag="LGK42_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK2657043.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7088..8521
                     /gene="dacB"
                     /locus_tag="LGK42_000765"
     CDS             7088..8521
                     /gene="dacB"
                     /locus_tag="LGK42_000765"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBK2657044.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8561..9733)
                     /gene="cgtA"
                     /locus_tag="LGK42_000766"
     CDS             complement(8561..9733)
                     /gene="cgtA"
                     /locus_tag="LGK42_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBK2657045.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9749..10714)
                     /locus_tag="LGK42_000767"
     CDS             complement(9749..10714)
                     /locus_tag="LGK42_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2657046.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(10841..11098)
                     /gene="rpmA"
                     /locus_tag="LGK42_000768"
     CDS             complement(10841..11098)
                     /gene="rpmA"
                     /locus_tag="LGK42_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK2657047.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11119..11430)
                     /gene="rplU"
                     /locus_tag="LGK42_000769"
     CDS             complement(11119..11430)
                     /gene="rplU"
                     /locus_tag="LGK42_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK2657048.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11689..12660
                     /gene="ispB"
                     /locus_tag="LGK42_000770"
     CDS             11689..12660
                     /gene="ispB"
                     /locus_tag="LGK42_000770"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBK2657049.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            12888..13166
                     /gene="sfsB"
                     /locus_tag="LGK42_000771"
     CDS             12888..13166
                     /gene="sfsB"
                     /locus_tag="LGK42_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBK2657050.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13214..14473)
                     /gene="murA"
                     /locus_tag="LGK42_000772"
     CDS             complement(13214..14473)
                     /gene="murA"
                     /locus_tag="LGK42_000772"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK2657051.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14528..14782)
                     /gene="ibaG"
                     /locus_tag="LGK42_000773"
     CDS             complement(14528..14782)
                     /gene="ibaG"
                     /locus_tag="LGK42_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBK2657052.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(14942..15235)
                     /gene="mlaB"
                     /locus_tag="LGK42_000774"
     CDS             complement(14942..15235)
                     /gene="mlaB"
                     /locus_tag="LGK42_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBK2657053.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15235..15870)
                     /gene="mlaC"
                     /locus_tag="LGK42_000775"
     CDS             complement(15235..15870)
                     /gene="mlaC"
                     /locus_tag="LGK42_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBK2657054.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(15889..16440)
                     /gene="mlaD"
                     /locus_tag="LGK42_000776"
     CDS             complement(15889..16440)
                     /gene="mlaD"
                     /locus_tag="LGK42_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBK2657055.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16445..17227)
                     /gene="mlaE"
                     /locus_tag="LGK42_000777"
     CDS             complement(16445..17227)
                     /gene="mlaE"
                     /locus_tag="LGK42_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBK2657056.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17235..18044)
                     /gene="mlaF"
                     /locus_tag="LGK42_000778"
     CDS             complement(17235..18044)
                     /gene="mlaF"
                     /locus_tag="LGK42_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBK2657057.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18254..19231
                     /locus_tag="LGK42_000779"
     CDS             18254..19231
                     /locus_tag="LGK42_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBK2657058.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19245..20231
                     /gene="kdsD"
                     /locus_tag="LGK42_000780"
     CDS             19245..20231
                     /gene="kdsD"
                     /locus_tag="LGK42_000780"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBK2657059.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20252..20818
                     /gene="kdsC"
                     /locus_tag="LGK42_000781"
     CDS             20252..20818
                     /gene="kdsC"
                     /locus_tag="LGK42_000781"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBK2657060.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20815..21390
                     /gene="lptC"
                     /locus_tag="LGK42_000782"
     CDS             20815..21390
                     /gene="lptC"
                     /locus_tag="LGK42_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBK2657061.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21359..21916
                     /gene="lptA"
                     /locus_tag="LGK42_000783"
     CDS             21359..21916
                     /gene="lptA"
                     /locus_tag="LGK42_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBK2657062.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            21923..22648
                     /gene="lptB"
                     /locus_tag="LGK42_000784"
     CDS             21923..22648
                     /gene="lptB"
                     /locus_tag="LGK42_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBK2657063.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22696..24129
                     /gene="rpoN"
                     /locus_tag="LGK42_000785"
     CDS             22696..24129
                     /gene="rpoN"
                     /locus_tag="LGK42_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK2657064.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24152..24439
                     /gene="hpf"
                     /locus_tag="LGK42_000786"
     CDS             24152..24439
                     /gene="hpf"
                     /locus_tag="LGK42_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBK2657065.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24557..25048
                     /gene="ptsN"
                     /locus_tag="LGK42_000787"
     CDS             24557..25048
                     /gene="ptsN"
                     /locus_tag="LGK42_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBK2657066.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25094..25948
                     /gene="rapZ"
                     /locus_tag="LGK42_000788"
     CDS             25094..25948
                     /gene="rapZ"
                     /locus_tag="LGK42_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK2657067.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            25945..26217
                     /gene="npr"
                     /locus_tag="LGK42_000789"
     CDS             25945..26217
                     /gene="npr"
                     /locus_tag="LGK42_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBK2657068.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26430..27062
                     /gene="yrbL"
                     /locus_tag="LGK42_000790"
     CDS             26430..27062
                     /gene="yrbL"
                     /locus_tag="LGK42_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBK2657069.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27059..27787)
                     /gene="mtgA"
                     /locus_tag="LGK42_000791"
     CDS             complement(27059..27787)
                     /gene="mtgA"
                     /locus_tag="LGK42_000791"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBK2657070.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27784..28437)
                     /gene="elbB"
                     /locus_tag="LGK42_000792"
     CDS             complement(27784..28437)
                     /gene="elbB"
                     /locus_tag="LGK42_000792"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBK2657071.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28667..31003)
                     /gene="arcB"
                     /locus_tag="LGK42_000793"
     CDS             complement(28667..31003)
                     /gene="arcB"
                     /locus_tag="LGK42_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBK2657072.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31099..32028)
                     /locus_tag="LGK42_000794"
     CDS             complement(31099..32028)
                     /locus_tag="LGK42_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK2657073.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            32703..37163
                     /gene="gltB"
                     /locus_tag="LGK42_000795"
     CDS             32703..37163
                     /gene="gltB"
                     /locus_tag="LGK42_000795"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBK2657074.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37176..38594
                     /gene="gltD"
                     /locus_tag="LGK42_000796"
     CDS             37176..38594
                     /gene="gltD"
                     /locus_tag="LGK42_000796"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBK2657075.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38778..39905
                     /locus_tag="LGK42_000797"
     CDS             38778..39905
                     /locus_tag="LGK42_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBK2657076.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERILLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(39965..40429)
                     /locus_tag="LGK42_000798"
     CDS             complement(39965..40429)
                     /locus_tag="LGK42_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBK2657077.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40426..41301)
                     /gene="nanK"
                     /locus_tag="LGK42_000799"
     CDS             complement(40426..41301)
                     /gene="nanK"
                     /locus_tag="LGK42_000799"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBK2657078.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41298..41987)
                     /locus_tag="LGK42_000800"
     CDS             complement(41298..41987)
                     /locus_tag="LGK42_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK2657079.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42035..43525)
                     /gene="nanT"
                     /locus_tag="LGK42_000801"
     CDS             complement(42035..43525)
                     /gene="nanT"
                     /locus_tag="LGK42_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBK2657080.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSPKNDLVKTKS"
     gene            complement(43634..44527)
                     /gene="nanA"
                     /locus_tag="LGK42_000802"
     CDS             complement(43634..44527)
                     /gene="nanA"
                     /locus_tag="LGK42_000802"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBK2657081.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44649..45440)
                     /gene="nanR"
                     /locus_tag="LGK42_000803"
     CDS             complement(44649..45440)
                     /gene="nanR"
                     /locus_tag="LGK42_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBK2657082.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            45820..47187
                     /gene="dcuC"
                     /locus_tag="LGK42_000804"
     CDS             45820..47187
                     /gene="dcuC"
                     /locus_tag="LGK42_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBK2657083.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVLLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(47230..47727)
                     /gene="sspB"
                     /locus_tag="LGK42_000805"
     CDS             complement(47230..47727)
                     /gene="sspB"
                     /locus_tag="LGK42_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBK2657084.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47733..48371)
                     /gene="sspA"
                     /locus_tag="LGK42_000806"
     CDS             complement(47733..48371)
                     /gene="sspA"
                     /locus_tag="LGK42_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBK2657085.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(48766..49158)
                     /gene="rpsI"
                     /locus_tag="LGK42_000807"
     CDS             complement(48766..49158)
                     /gene="rpsI"
                     /locus_tag="LGK42_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK2657086.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(49174..49602)
                     /gene="rplM"
                     /locus_tag="LGK42_000808"
     CDS             complement(49174..49602)
                     /gene="rplM"
                     /locus_tag="LGK42_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK2657087.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(49821..50948)
                     /gene="zapE"
                     /locus_tag="LGK42_000809"
     CDS             complement(49821..50948)
                     /gene="zapE"
                     /locus_tag="LGK42_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBK2657088.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            51142..51540
                     /locus_tag="LGK42_000810"
     CDS             51142..51540
                     /locus_tag="LGK42_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBK2657089.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51694..53061
                     /gene="degQ"
                     /locus_tag="LGK42_000811"
     CDS             51694..53061
                     /gene="degQ"
                     /locus_tag="LGK42_000811"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBK2657090.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            53151..54218
                     /gene="degS"
                     /locus_tag="LGK42_000812"
     CDS             53151..54218
                     /gene="degS"
                     /locus_tag="LGK42_000812"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBK2657091.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(54280..55218)
                     /gene="mdh"
                     /locus_tag="LGK42_000813"
     CDS             complement(54280..55218)
                     /gene="mdh"
                     /locus_tag="LGK42_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBK2657092.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            55653..56123
                     /gene="argR"
                     /locus_tag="LGK42_000814"
     CDS             55653..56123
                     /gene="argR"
                     /locus_tag="LGK42_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBK2657093.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        13848 a        15129 c        14024 g        13317 t
ORIGIN      
        1 aactggagtt gacacaataa agtgccaatt atgtcagcag aagggaaaag tttataacta
       61 aggcgtaccg gcaccatcgt ttcaaggtac cagctacgag taaagcaact ggacgagata
      121 cagatacctg acaaccattc ctcagaccag gaccaaaacg aaaaaagacg cttttcagcg
      181 tctcttttct ggaatttggt accgaggacg ggacttgaac ccgtaagccc tattgggcac
      241 taccacctca aggtagcgtg tctaccaatt ccaccacctc ggcacggata ctacttttta
      301 gttcgggata tcgctggtcg gcttagccgg agcagctggt tgagtttgtt cggttttcac
      361 cggtgcactc agattttccc attcgctacc tttattggtt ttgttgctat tgatgttacc
      421 cagcaccaga ctgatgatga agaataacgt tgccagcagc gccgtcatgc gggtcatgaa
      481 gttaccagaa ccacttgaac caaacagcgt agcggaagcg cctgctccga aggaggctcc
      541 catatcagcg cctttacctt gctgcagcat gatcagacca acaaggccaa ttgccacaat
      601 aaggaaaact actaaaagag cttcgtacat aatcaacctg ttccttgcgg agttgccgcg
      661 taccaatgct tcaaccaata aagcgggagt ttttaatctt tcccactgaa gcgggtgtga
      721 atactaacca aagcgaatga ccttcgcaag ggcaatttta tcgcattgta tcaactgcgg
      781 aaaaaaacag caaaaccgat gtgttcgctg agaaaaaagg cgacaatcgc cgccttttta
      841 gccagttatc taacgcttta aacggctttt actgcatcgg cgatgcggtg tgcaaattca
      901 gtcacctgcg cttcgtcttc gccttccacc atcacgcgaa ttaacggttc ggtgccggat
      961 ttacgcagca acacgcgtcc acggttgccc agcgcagctt caacctctgc ggtcacggct
     1021 ttaactgact catgctcaag tggatcgccg ctacctgcgg tgtaacgtac gttaaccaga
     1081 atctgcggga acattttcat gccgctgcaa aggtcgtgca ggctcatatg gttacgtgcc
     1141 atcgccgcca gcacctgcaa gccagcaacg atgccgtcac cggtagtagt tttatccagt
     1201 aggatcacat gaccggaatt ctctgcaccg atacgccagc ctttctcctg cattttttcc
     1261 agtacgtagc ggtcacccac tttcgcgcgc gcaaatggaa ttcctaactg tttcagcgcc
     1321 agttcaagcc ccatgttgct catcaatgta cccacagcgc caccacgcag ctggccctga
     1381 cgaagacctt cacgcgcgat gatatacatg atctgatcgc catcgacttt attgccttca
     1441 tggtcgacca taatcacgcg atcgccatcg ccgtcgaagg caataccgag atccgctttt
     1501 tcagccagca cacgagcctg gagcgcgcga acgtcggtag cccccacttc ggcattgatg
     1561 tttacaccgt ttggctcaca accgatagcg ataacgttcg cccccagttc gcgcagcacg
     1621 ttcggcgcga tgtgataagt cgcaccgttt gcacaatcca ccacaatctt cagttcactg
     1681 aggctaagtt cgttcgggaa cgtggctttg caaaactcga tatagcgacc cgcggcatca
     1741 acgatacggc tggctttacc cagttctgcc gaatcaacgc agctgatctc cttttccatt
     1801 tccgcttcga tggcctcttc taccgcatcc ggcagtttgg tgccgtcgat agagaagaat
     1861 ttaatcccat tatcgtagaa cgggttatgc gatgcagata tcacaattcc ggcctctgcg
     1921 cggaaggtac gcgtcagata agccacggcc ggtgttggca tcgggccagt gaagagtgcg
     1981 gaaaggcccg ctgccgccag acccgcttcc agtgctgact ccagcatata gccagaaata
     2041 cgcgtgtctt taccaataat aatcttacgg gagccgtggc gcgccagcac tttacccgcg
     2101 gcccaaccca gcttaagcac aaaatcaggt gtgatcggcg catcccctac acgaccacga
     2161 atcccatcgg taccgaaata tttacgatta ctcatagcgt ttgttttcct ttgcagacag
     2221 agtggcttcc accacccgca tcgcttctac ggtttctttg acgtcatgaa cacgaatgat
     2281 gtgcgcgcct tgcattgcgg caatgaccgc acaggccaga ctaccgctca ggcgctcgga
     2341 cggccccacg ttcagcagct gcccaatcat cgattttcgt gacataccca ccaacagcgg
     2401 caggttgaaa tggtgaaatt cagccaggcg cgccagtaat gaatagttat gggagagatt
     2461 tttaccgaaa ccgaatccgg ggtcgagcaa caatttctct tttgcgatac ccgcctgctc
     2521 gcaacgtgct atttgctcaa taaagtagcg attcacttct gcaaagacat cgtcatactt
     2581 cggagcttcc tgcatggttt ttggatttcc ctgcatatgc atcagacaaa ccggtaaacc
     2641 ggtttctgca gccgcctcca gagcgccagg ttcggaaagg gagcggatat cattaataat
     2701 gtgagcgcca actttcgctg actcacggat gacttctggt ttggatgtat cgaccgagat
     2761 ccagacttcg aagcgttgag caattgcctc aaccacagga ataacacgtt gcaactcttc
     2821 ttcaacgcta acttccgccg cccctgggcg cgtggactcg ccaccaacgt caatgatcgt
     2881 cgcgccagca ttgatcatca gattcgcatg tttcaccgca tctatcagcg agttatgcgt
     2941 gccaccatcc gaaaaggaat caggcgtgac gttgaggatc cccattacgt gaggatggct
     3001 aaggtccagt gaagtaccct gggcaaagag tttcatgatg ttatccctgg tatgaattga
     3061 taagaaaaaa ccccggagca cgccccgggg ttttcggtac aaatacagtc atctgatgcg
     3121 ggaacttact tgtcgcctaa ctgctctgac atggtgttac ccgggttcgg cgtacgcggt
     3181 tcatcaaccg gacgaggagc ctttggacta ccattgtcgc cagaattgtt agaagcgcct
     3241 ggttcttccc agcccgctgg cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc
     3301 ggtgcgtcga tagtctcata tttcatgaga gcatctttca tcgcatgcag aatatccata
     3361 ttgtcggtca gaagctgacg cgcacgatta tagttacgct caatcagtgc tttcacttcc
     3421 tggtcgatga tacgtgcagt ttcatcggac atatgtttcg ctttcgctac gctacggccg
     3481 aggaatactt caccttcttc ttccgcgtac agcagtggac ccaatttctc agagaagccc
     3541 cactgggtca ccatgttacg tgccagattg gtcgcaactt taatatcgtt ggacgcaccg
     3601 gtagaaacat gttccggccc atagatgatc tcttctgcca gacgaccacc gtacagcgta
     3661 gaaatctggc tttccagttt ctgacggctg gcgctgattg cgtcgccctc aggcaagaag
     3721 aaagtcacac ccagcgcacg accgcgtggg ataatcgtca ctttgtgcac cggatcgtgt
     3781 tccggcacca ggcgaccgat aatcgcatga cccgcttcgt ggtaagccgt tgattctttc
     3841 tgcgcttccg tcatcaccat ggagcgacgt tccgcaccca tcatgatttt gtctttcgct
     3901 ttctcgaact caaccatcga cacaacgcgt ttgttgccac gagcagcgaa cagtgccgct
     3961 tcgttcacca ggttcgccag gtcagcaccg gagaaaccag gagtaccacg ggcaatgatt
     4021 gccgcgtcga tatcgggggc caatggtacg cgacgcatgt gaactttcag gatctgctca
     4081 cgaccgcgaa catctggcaa gccgaccaca acctgacggt cgaaacggcc aggacgcagc
     4141 agggccgggt cgagaacgtc cggacggtta gtcgcggcga taacgatgat accttcgtta
     4201 ccttcgaagc catccatctc aaccagcatc tggttcagag tctgttcacg ttcatcgtga
     4261 ccaccgccca gaccagcgcc acgctggcgg cctacggcgt cgatttcatc gataaagatg
     4321 atgcacggtg ccgctttctt cgcctgttcg aacatgtcac gaacacggga tgcacccaca
     4381 ccgacgaaca tttctacgaa gtcagaaccg gagatagtaa agaacggaac tttcgcttcg
     4441 cctgcaatcg ctttcgccag caacgtttta ccggtacccg gaggaccgac catcaggacg
     4501 cctttcggga tcttaccgcc gagtttctgg aagcggctcg gctcgcgcag atactctacc
     4561 agttcagcaa cttcttcttt tgcttcgtcg cagcccgcaa cgtcagcaaa ggtcgttttg
     4621 atctgatctt ccgtcagcat gcgcgctttg ctcttaccaa acgacatggc acctttgcca
     4681 ccgccgccct gcatttgacg catgaagaag atccagacac caatcagcaa cagcatcggg
     4741 aaccaggaga tgaagataga agccagcagg cttggttctt caggcggttc accgacaacc
     4801 ttgacgttct tggtcaacag gttatccagt aatttcggat cctgaaccgg aatgtaagtg
     4861 gtataacggt tactatcttt cttggtaacg ttgatttcac gtccgttgat acgcgcttca
     4921 cgaacctggt cgttattgac ctcttgtagg aaggtagagt aatccacctt acggccatta
     4981 gactcgctgg gcccaaagct ctggaatact gacatcagca caacggcaat gaccagccag
     5041 agtattaggt ttttcgccat gtcactcaag ggattaacct cttattacaa ctgtgttaaa
     5101 aacagcgtca ggatactcta tatccagcat ctttcaaact ttcgtctgaa atctcccggt
     5161 tagggtttac gcccggtcgc tacaatatac acttcccgcg aacgtgcacg agaagagtcc
     5221 ggcttacgaa ctttgacctt cgtaaacagg gagcgaattt cccttagata ctcatcgaaa
     5281 ccttcgccct ggaacacctt cactacaaaa ctgccacctg gcgctaatac atcacgacac
     5341 atttctagcg ccagttccac cagatacatg gcacggggga tatccaccgc cggtgttccg
     5401 ctcatgtttg gtgccatatc ggacatgaca acctggactt tgctgtcgcc aacgcgctcc
     5461 agcagtgctt tcatcaccag ttcatcacga aaatcgccct gaagaaagtc cacaccaacg
     5521 ataggatcca taggtaaaag atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg
     5581 accacatatt gtgaccaacc acccggagca gcaccaaggt cgacaaccgt cattcccggt
     5641 ttaaagagtt tgtcactttg ctgtatttca tcaagtttaa accaggcacg ggaacgtaac
     5701 ccctttttct gtgcctgttg aacatattta tcgctaaagt gttcctgaag ccagcggctg
     5761 gagctggcag aacgcttctt acctgtcatt taactttccc atggggataa ctcatcgtaa
     5821 ccaattgcgt aaatttttac gcgcctattt ggtgatatat gggagatggc ggtagaatga
     5881 cccgttttca atcccaacgt aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca
     5941 gcacctgaaa ggtctggcac atccgctcaa gccagttgtt ctgcttggca gtaatggttt
     6001 gaccgaaggg gtgctggccg agattgaaca agcgttagag caccatgaac tcatcaaggt
     6061 gaaaatcgcc accgaagatc gcgaaactaa aaccttgatc gtggaagcta tcgtgcgcga
     6121 aaccggcgcc tgtaatgtac aggtcatcgg taaaacgctg gtgctttatc gcccaactaa
     6181 agaacgtaaa atctcgctgc cacgctaaga ttatcctaaa gttacacaca tttgctgtgt
     6241 aaaacgaggg gttttccgca ggcaggagag caaaatgcca cgctctgttc gttgataaaa
     6301 ggccgcatag cggccttttt cctttcttta caatacatca acatcttgag tattgggtaa
     6361 ttcttacagg tattccacct taattacttc aaattctact tcgccgcccg gcgttttgat
     6421 gaccacaaca tcatcttctt ctttgccgat caggccacga gcaataggcg agtttacaga
     6481 aatcaggttt tgtttaaagt cagcttcgtc atcgccaacg atgcgataag tctgttcttc
     6541 gtcagaatcc agattcagca ccgttacggt agcaccaaaa ataacgcgcc cattgttggg
     6601 cattttggtg acatcaatca cctgcgcgtt cgacagcttg gcttcgatgt ctttaatacg
     6661 gccttcgcag aaaccctgct gttcacgagc tgcgtggtat tcggcgtttt ctttcaggtc
     6721 gccatgctca cgcgcttccg cgatagcagc aatgatttca ggacggcgca cagatttcag
     6781 aaaatccagc tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa tagcttgcat
     6841 ttgttatacc tcttgaatat tcctgatggg gcaagtcttc acccaccccg gctgttcggc
     6901 ctgcccggca taactcctca ccgggaccag aagcaaaaaa ataccgaccc gggtacaagt
     6961 cccaggtcag ctacaattca cattttgata gtcattttac cctgaagttc ccgaagggtc
     7021 atcgtttact ttatagggcg ttgcgccgta gtatgacggc tcgattccag gttgttagcg
     7081 cgagattatg cgattttcca gatttatcat cggattgacc agctgtatag cgttcagtgt
     7141 tcaggccgcg aatgttgatg agtacgttac tcaactcccc gctggtgcca accttgccct
     7201 gatggtgcaa aaagtcggcg cgtcggcccc cgctattgat taccacagtc agcagatggc
     7261 gctgcccgcc agtacgcaga aagtgattac tgcgctggcg gcgttgattc aactcggccc
     7321 cgattttcgt ttcaccacga cgcttgaaac caaaggcaat gtggaaaacg gcgtacttaa
     7381 gggtgactta gtggcgcgat ttggtgccga tccgacgtta aaacgtcagg atattcgcaa
     7441 tatggtcgcg actttgaaaa aatctggcgt caaccaaatc gatggcaatg tgttgataga
     7501 tacctccatt ttcgccagcc acgataaagc ccccggctgg ccatggaatg acatgacaca
     7561 atgctttagc gctccgcctg ccgctgccat agttgaccgc aactgtttct ccgtctcgct
     7621 ctacagtgcc ccaaaacctg gtgatatggc ttttatacgc gtggcatctt attaccccgt
     7681 tacgatgttc agccaggtac gcaccctccc ccgaggttct gccgaagcgc aatactgcga
     7741 actggatgtc gtaccaggcg acctgaaccg ctttacgctg acgggatgcc tgccacaacg
     7801 ttctgagccg ctcccgttgg cttttgccgt gcaggatgga gccagctatg ccggggcaat
     7861 tctgaaagat gagttaaaac aggcaggtat cacctggagc ggaacactgc tgcgccagac
     7921 tcaggttaac gaacctggaa cggtagttgc cagtaaacag tcggccccgt tgcacgatct
     7981 gcttaagatt atgctgaaaa agtcggacaa catgatcgcc gatacggttt tccgcatgat
     8041 aggccatgcg cgcttcaatg tgcctggaac atggcgggcc gggtcggacg ccgtgcgtca
     8101 gatcctgcgc cagcaagccg gtgtcgatat tggaaacacc attattgccg atggttcagg
     8161 gctttcgcgg cataacctga ttgcccccgc caccatgatg caggtgctgc aatacattgc
     8221 ccaacacgac aatgaactta actttatctc catgctgccg cttgcgggct atgacggctc
     8281 tttgcagtac cgtgcaggtc tgcatcaggc gggcgtggat ggaaaagtct cagcgaaaac
     8341 cggttcgttg cagggggtat ataacctggc gggattcatt accacagcga gcgggcaacg
     8401 aatggcgttt gtacaatatc tttctggcta tgcagtagaa ccggcggatc agcgtaatcg
     8461 ccgtattccg ttagtgcgtt ttgaaagccg tttgtataaa gatatttatc agaacaatta
     8521 gtcaaaaaga aaccccggca catggccggg gttttgctga ttaacgcttg taaatgaact
     8581 caacgccttc ttcgtcgtct tcgtcccagt catcatccca gtcctcatca tcctcttcag
     8641 caatctcttc aagctgctgg cgatgataat catcccacat gaattcgact ttctctggct
     8701 gtttcgcttc ttcagcctgc acgaccgggt tttcaatgat aaaggtcatc acatcccagc
     8761 agagatcttt cacgcccagt ccactcgccg cagagatcag ataatattta tcttcccagc
     8821 ccagcgcttc agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga
     8881 tcttattgaa cactaaccaa cgtggtttcg ccgccagatc ctggctgtat ttttccagct
     8941 cgctgataat aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga
     9001 tgaggtgcaa caggacgcgg caacgttcca ggtgcttcag gaagcgaatg cccagacctg
     9061 cgccttccgc agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt
     9121 tgtccattcg taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt
     9181 tcggtttagc cgccgatacc gcacgaataa aggtcgattt acccgcgttt ggcatcccca
     9241 acataccgac gtcagccagc agcatcagct ccagcagcag ctcacgctta tcgcccggcg
     9301 taccgttagt tttctgccgt ggtgtacggt taactgacga tttgaaacgg gtattgccca
     9361 gaccgtgcca gccgccctta gcaaccagca gacgctgacc gtgtttggtc atatcgccca
     9421 tggtttcacc agtaccctgg tcgattacac gcgtacctac cggcacttta attgtgacgt
     9481 ctttaccgcg cttaccggta cagtcgcggc ttgcgccatt ctgaccgcgc tctgcacgga
     9541 aagatttttc aaaacgataa tcgataagcg tgttcaggtt ctcgtcggct tccatccata
     9601 cgtcaccacc atcaccgccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc
     9661 ggcggaagct cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat
     9721 caacaaactt cattttattc tccgtaaatc attcgcctgc gcggggttgc gataccaccg
     9781 ttgtatgctt acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg
     9841 caaccacgac aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat
     9901 cgggccaggc cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga
     9961 tgatcgcgct cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc
    10021 ctaccaaggt attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg
    10081 ctatcactcc aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa
    10141 acaggatctg cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca
    10201 cccaaaccgt cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg
    10261 taaatatctc gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg
    10321 cccctacaac ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca
    10381 tgccaactgg cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg
    10441 atgagctgaa caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca
    10501 accagcgtgg cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg
    10561 caccaaggcc aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca
    10621 cctccagcac ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg
    10681 ccaaaagaat gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg
    10741 cggattaacg tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac
    10801 gtgttgcggg gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga
    10861 taaatttacg gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa
    10921 acagagtgtg gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt
    10981 gacgaacgat gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc
    11041 gtttagcttc tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt
    11101 gaaatctctc ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg
    11161 acgatggccc tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt
    11221 ctcgccacga ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac
    11281 gccgattttg acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc
    11341 gccagttgcg atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg
    11401 tttaccacca ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc
    11461 aatgattcag agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac
    11521 gcctttgaca agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg
    11581 tttatctgtg ccattttttc agtacaatca ctctatattc ctaaccataa accctaagtt
    11641 gcctttgttc acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa
    11701 aaaatcaatg agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag
    11761 cttaattccg acgtccagct gatcaatcag ttaggctatt acatcgtcag cggcggcggt
    11821 aaacgtattc gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat
    11881 gcgcatgtca ccattgctgc cctgatcgag tttatccaca cggcgactct gctgcacgac
    11941 gacgttgtgg atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc tgcatttggc
    12001 aatgccgcca gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc
    12061 agcctcggtt cgctcaaagt gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa
    12121 ggtgaagttc tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg
    12181 cgcgttatct atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt
    12241 ctggctggct gtacgccgga agaggagaaa ggcctgcagg attatgggcg ctatctcggc
    12301 actgctttcc agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt
    12361 aaaaatgtcg gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg
    12421 catcatggca caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt
    12481 cgccatcttc tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg
    12541 cgtcagcgtg ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac
    12601 accccttggc gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa
    12661 tcccctccct tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact
    12721 aattcagcgc tataacacct gaaaaaaagg gcgatgaatc tttatatatt ctgaatattc
    12781 acactcttta caggaacttt ttagagcaat aggccatcag gagtatagtg atgcccgaca
    12841 gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg aggaaagatg gaaagtaatt
    12901 tcattgactg gcatcccgct gacatcattg cgggtttgcg caagaaggga acgtcaatgg
    12961 cggcggagtc tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca ttatcgcgcc
    13021 catggccgaa aggagagatg attattgcga aagccctggg aactgacccc tgggttatct
    13081 ggccatcacg ctaccatgat ccgcagaccc atgagtttat cgacagaacg cagttgatgc
    13141 gtagctacac taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa cggggctgcc
    13201 agctctcaga cgattactcg cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt
    13261 tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat acgatcaacc accgtcgtcc
    13321 cttccgcaat acagccagcc agcaccaggc ttgctgatgc acgcagatcg gttgccataa
    13381 cctgtgcgcc agaaagtttt tcaacaccgt gacaaataac ggtattgctt tcgatttcgg
    13441 catgcgcgcc catacggcta agctccggca catgcataaa gcggttttca aagaccgttt
    13501 cggtgatgaa tccggtccct tctgccacca ggttcaacag cgtgaactgg gcctgcatat
    13561 cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac agccttcgga cgtttgccat
    13621 gcatatccag gctaatccag tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt
    13681 tcgccagcac ggcgtcgaga gtatctggct gcgcgttacg gcagataatt ttgccgcgag
    13741 aaatcgccgc cgccaccagg aaagtaccgg tttcgatacg atccggtagc acgcgataga
    13801 caccgccgcc taaacgttcc acaccttcga tgacgatacg atcggtgccc tgaccgctaa
    13861 ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac gatttccggt tcacgcgctg
    13921 cgttttcaat aatcgtggta ccttccgcca gggttgcagc acacatgatg gtcaccgttg
    13981 cgccaacgct gactttatcc atcacgatat gtgcgccttt cagacgacca tcgacggaag
    14041 ctttaacgta accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt
    14101 gtagatcaac cggacgcgca ccgatcgtac aaccgccagg cagtgaaact tgcccctgac
    14161 caaagcgcgc taccagcggc cccagtgccc agatagaagc acgcatggtt ttaaccagat
    14221 cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt
    14281 ctactttcgc acccagctgg cttagcagct tcattgatgt atcgacgtct ttcagttttg
    14341 ggacgttctg gatctctacc ggttcttccg ccagtagagc ggcaaaaagg ataggcagag
    14401 cagcattttt agcgccggaa attgtgactt cgccctggag cttcgttggc ccctgaacac
    14461 gaaatttatc catttagttt gttctcagtt aacaattcat atccgctacc ggcgaatcgc
    14521 ccatagctca aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt
    14581 tgatcgacac agcatgaatg cggttatccg caatatattc catcagcggg ccatagaccg
    14641 tctgctgttt tttaacccga ctcatgccgt caaacaactc acccacggca ataacctgaa
    14701 agtggctgcc atcgccggaa acgtggactt cctggaggga gagagcgttc atcaacacgc
    14761 tctgaatttc attattttcc atgggatctt caatcatcag ttaataaacc agcgaaacat
    14821 cttagagcaa agttgcgctg gcataaataa gcaaaaagcc tcgctgataa atcagacaag
    14881 gctcgacttg caggcaggtt tgccggacag gcggttaacg ccatatccgg cctgaaaaaa
    14941 tttaacgagg cagaacatca gcaggcaaat tatacaattt cgccagggta tacactttgt
    15001 cgtttacccc ctgaagcgtc acattgttgc cctgcttttt cgccagatcg ataagatgga
    15061 gcagcagtgc cagtcccccc gtatccacgc gggacacacg gctaagatcg atgcaggtaa
    15121 tccccttcac cgcttcctca cgcatttccc aaagcggtag caaaacgtcc tgatccagct
    15181 ctccggataa cgccagcgtg tcacccgtct gcatccagct cagtgactcg ctcattattt
    15241 tttctcttcc agagtgattt tctgttgaga aatcgatttc agttgcgcag tcaggccgtc
    15301 gatacctttg gtacgcagca gcgttcccca ctcgttttgt ttggtggtga tcatactgac
    15361 gccttcagca atcatgtcgt aagcctgcca gttgcccgtc tgggagtttt tacgccactg
    15421 gaagtccaga cgcaccggcg ggcggccatt cggatcaata atggtaacgc gaatcggcac
    15481 aatggtttta tcgcccagcg gctgttctgg cgcaatctga taggtttgac cgtgatacat
    15541 cgccagcgcc tgaccgtaag cctgctttag gtactcacgg aaagcggcaa agtaggcttc
    15601 acgttgagca ggggtcgcac tcttgtaata ctggcccagc accagcgcac cggcgtattt
    15661 cacctgtacg tatggcagca gttcctgatc aacaatggta cgcagataat ccgggttggc
    15721 ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat
    15781 cagcttataa ggattggtct ggtctgccgc ggttgccgca ctcagaggtg caatcaccag
    15841 caaagcgacc atcattaaac gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt
    15901 acccacaggt tcagtggttt cattattacc tggcgcagca gctggcgcat cgccactatt
    15961 cttattgtca tcgcctttac taccgtaaag gaactgacca atgagatctt ccagcaccat
    16021 cgcagactta gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc ccagttccgg
    16081 gtcttcaaaa ccgacgttta atgccagata ttgttccccc agcaggccgg aagtacgaat
    16141 gctcagcgaa ctggtatctg gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg
    16201 cggcagatag gttttcgggt ccagcgtaat atccgccacc cgacccacaa caacgccacc
    16261 aatactgacc ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt
    16321 gtaggtcggt tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc
    16381 cgccagcagt gctgctaata aaaagatacc cacccaaatt tcattttttt tcgtttgcat
    16441 gaactcaatt cccaaacatc aatgcggtca gcacaaaatc cagccccaga acagccagag
    16501 acgagtggac aacggtgcga gtggttgccc ggctaatccc ggcagacgtc gggatggcgt
    16561 cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc gaacaccacg ctcttaatca
    16621 gacagttgac cagatccata cgccagtcga cggcattttg cattgccgac cagaagaacc
    16681 cgctatcaat gcctttccaa ctgacgccga ccagagatcc gccccagatc cccacggcga
    16741 cgaaaataac cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa
    16801 cccgacgcag cggatccacc gccatcatct ccatactgga gagttgctct gtagcgcgca
    16861 tcaggccgat ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa
    16921 ccaccggccc cagttcacgc agtagcgata acgccaccag catacccaga ctggtttccg
    16981 cactataagt ggtcagaacc agataacctt gcagccccaa caccattccg atgaacacgc
    17041 cagaaaccac aataatcagc atcgacagga cgccgacatt atagagctgg cgcaccagca
    17101 gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc attgaataac attaacccgg
    17161 cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc agcgcattta
    17221 acagcatgag tggcttaact ccctggtaaa agatcagcgt gataatcgcc ggcaggatag
    17281 cggaacggaa caggcccgtc agctatcccg tccagaaact gacgtacgcg cggatcagga
    17341 ttcgcctgca acgcctgggc actgccatga gcgacaattt ttttgtccgc caggatccag
    17401 gcgtgatccg caatacttaa cacttccggc acatcgtgag aaaccaccac acaagtcacg
    17461 cccagcgcgc tgttcagctc agaaatcagc ttcaccagta cgcccatggt gatgggatct
    17521 tgcccaacaa aaggttcatc aaacatgatg agatccggct ccagcgcaat cgcacgcgcc
    17581 agcgctgcac gccgcgccat cccaccggaa agttcagaag gcattagttt agccgctcca
    17641 cgcagcccca cggcctccag cttcatcatc accgtactat gcaacaatgg cgcgggaagt
    17701 tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc agtgaacaac
    17761 gccccggact gaaataacat gctcatccgt ttgcgcactg tatacaggcg cgaacgagac
    17821 atcgccggaa tattctcacc atcgaaaagg atctcaccat gatctggtgc gatttgcccg
    17881 ccaatcagac ggagtagcgt cgttttaccg atgcccgatg gccccatgat cgccgtgatc
    17941 ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa
    18001 ctgacatcgc gcatatcgac taaattcgcc acagactgct ccataattca cccttcgtct
    18061 tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga acccaacata tttacagaat
    18121 attacccgcc gtggttagcg aaagctggca tttgttttac tttttagccg cataaagtca
    18181 aaattaagaa tccgttacgg ctttctgaaa atcttcagcg gaccggcgag tatacctgaa
    18241 gaaaggacgt tagatgcttt tagctacggc actgttaatt gttggtttac ttttggtcgt
    18301 ttacagtgcc gaccgcctgg tttttgccgc gtctattctt tgccgaacct ttggcatccc
    18361 gccgctgatc atcggcatga cggtggtcag tattggtaca tcgttaccag aaatcatcgt
    18421 ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc gttggtacag ccctcggctc
    18481 aaacattatc aatatattgc tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt
    18541 ccattctgat gttctacgcc gtgaattacc cttaatgttg ttggtcagcg tggtggccgg
    18601 ttccgtactc tatgacggac aacttagtcg cagcgatggt atctttctcc tctttctggc
    18661 tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc aggggactga
    18721 cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt
    18781 tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa
    18841 cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat
    18901 tgctatcgga accagcctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga
    18961 aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt
    19021 gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca gtcgtgacta
    19081 cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca
    19141 accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt
    19201 gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt
    19261 tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat
    19321 gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcttgt gaaaagatgt
    19381 tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca
    19441 aaatggcggc aacgtttgcc agcaccggta caccttcatt tttcgtccat cctggtgaag
    19501 ccgcgcatgg tgatttaggc atggttaccc cacaggatgt ggtgattgct atctctaact
    19561 caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt
    19621 taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt
    19681 gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg
    19741 ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg
    19801 ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg
    19861 taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc
    19921 gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca
    19981 atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg
    20041 tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc
    20101 ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga
    20161 tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta ctgcgtgcag
    20221 gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac
    20281 ctgttacggc cctgtcagcg ccgacgttat agcaaaagca gagaacattc gtctgctgat
    20341 cctcgatgtc gatggcgtac tgtcagatgg cctgatttat atgggcaata atggcgaaga
    20401 gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat
    20461 tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt
    20521 ggggatcact cacttgtatc aggggcagtc aaataaactg atcgccttta gcgatctgct
    20581 ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg
    20641 gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat
    20701 cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg
    20761 cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt
    20821 aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt
    20881 aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat
    20941 aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg
    21001 attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta
    21061 cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag
    21121 ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc actcgtgccg
    21181 gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat
    21241 gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa
    21301 atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat
    21361 gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc
    21421 cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga
    21481 ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac
    21541 ccagggcacc atcaaaatta atgccgacaa agtggttgtt acccgtccgg gcggcgaaca
    21601 aggtaaagaa gtgattgacg gctacggtaa accggcaacg ttctaccaga tgcaggacaa
    21661 cggtaaaccg gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt
    21721 cgttctgacg ggtaatgctt atctgcagca ggttgatagc aacattaagg gcgataagat
    21781 cacttacctg gtaaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac
    21841 caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca
    21901 gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta
    21961 taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg
    22021 tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt
    22081 gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca
    22141 tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct
    22201 cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca
    22261 acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag
    22321 catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc
    22381 tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt
    22441 tatcgacatt aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac
    22501 tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg
    22561 gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt
    22621 ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag
    22681 agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg
    22741 acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag
    22801 gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa
    22861 atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa
    22921 aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt
    22981 acaccatccg gcaccagcgg tgactacatt gacgatgagc tgccggtcta tcagggcgaa
    23041 acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac
    23101 actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgacaccgg ttatctgact
    23161 gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt
    23221 gaagccgtcc ttaagcggat ccaacggttt gatccggtcg gtgtagcggc aaaagatctg
    23281 cgtgactgcc tgctaatcca actctcccaa ttcgataaaa ccacgccgtg gctggaagag
    23341 gccagactga tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta
    23401 atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg
    23461 ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat
    23521 gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg
    23581 cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac
    23641 agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc
    23701 cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt
    23761 gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa
    23821 atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc
    23881 atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg cggcgaagct
    23941 tcctccacgg cgattcgtgc gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa
    24001 ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc
    24061 cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag
    24121 ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt
    24181 cgagatcacc gaggcactgc gcgaatttgt tacagccaaa tttgccaaac ttgagcaata
    24241 ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc
    24301 agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat
    24361 gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca aacataaaga
    24421 taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg
    24481 gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag
    24541 gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa
    24601 cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat
    24661 cagcgagctg gcggcgaaac aacttagtct gccgcctcag gtggtttttg aagctatcct
    24721 gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa
    24781 actggaagaa gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc
    24841 tttcgatgcc atcgacaacc aaccggtgga tttacttttt gccctgctgg tgccggcaga
    24901 ccaaactaaa acgcacctgc atactctgtc actggtggcg aaacgtctgg cggacaaaac
    24961 catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga
    25021 taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga
    25081 ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt
    25141 cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt
    25201 gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg tcagcattga
    25261 tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga
    25321 cgctttctca ccgcaactcc tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta
    25381 cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat
    25441 cgacaaagaa agcgatttgc tggaacctct gcgttcgcga gcggatctga ttgtcgatac
    25501 ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg
    25561 tgaacgcgaa ctgacaatgg tctttgagtc tttcggcttc aaacacggta tccctatcga
    25621 tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg
    25681 tccaatgaca ggtcttgata agcccgtcgc cgcgttcctc gaccgccaca cagaagtaca
    25741 caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa
    25801 caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta
    25861 tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca
    25921 tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag
    25981 ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct
    26041 gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaatagcgt tattgcactg
    26101 ctgatgctgg attctgccaa aggacggcag attgaagttg aagccaccgg tccacaggaa
    26161 gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga agattaatct
    26221 tcatcacttt gacattcaaa tcccttcaag ctcccccgac aataagaaaa gcgcgtactg
    26281 aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc
    26341 ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag
    26401 atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa aatcccctgg
    26461 gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga
    26521 ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg
    26581 cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg
    26641 agacggattg cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaaccct
    26701 ccattacgct gaccgaattt gctgagcaat gccgttacga agaagacatt gcacaactgc
    26761 gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc
    26821 tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct
    26881 gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc
    26941 tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc
    27001 tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt
    27061 aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa
    27121 tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat
    27181 taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt
    27241 ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat
    27301 tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc
    27361 cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg
    27421 tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    27481 gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    27541 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    27601 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg
    27661 ctgagaaggg aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga
    27721 ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    27781 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    27841 tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac
    27901 cacgatatca tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc
    27961 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat
    28021 cgctggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    28081 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt
    28141 cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    28201 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt
    28261 ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    28321 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    28381 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    28441 gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc
    28501 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    28561 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat
    28621 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    28681 cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt
    28741 caatccattc accgacgtta tcttcccagg caggaaggtc cggagactga atttgctgac
    28801 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt
    28861 cctctacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    28921 ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    28981 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    29041 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    29101 gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    29161 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    29221 aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca
    29281 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    29341 cgacatcaac gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt
    29401 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    29461 catcatcgac ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    29521 tgccctgttc gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa
    29581 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    29641 aatacatggc gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca
    29701 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    29761 gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt
    29821 cggtaatgac ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt
    29881 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    29941 gttgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa
    30001 agatattccc cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg
    30061 cggtgagttc ggtatccagc agaatacggc tcaggccgac gataccgttc agcggtgtac
    30121 gcaattcgtg actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg
    30181 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    30241 tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg
    30301 ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg
    30361 tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca
    30421 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    30481 tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg
    30541 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    30601 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta
    30661 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    30721 ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    30781 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    30841 catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg
    30901 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    30961 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    31021 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    31081 ggaattcccc tcaacgacct actccgttgg aggtatccac ggacgttcca gcgccgatcc
    31141 ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc
    31201 gcaccacaga ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    31261 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc
    31321 aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg
    31381 cagcggatgc agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa
    31441 acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc
    31501 gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat
    31561 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc
    31621 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    31681 accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag
    31741 cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc
    31801 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    31861 ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc
    31921 acggttaggg cagctaaaac cgccgtgtag cgtcagctta tgcacctttt gcccataacg
    31981 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    32041 cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat
    32101 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    32161 cataatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat
    32221 tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt
    32281 tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt caggaattga
    32341 ctgtttctct aacgacttcc cttttagcct taaagataaa atccatttta atttcagtaa
    32401 tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    32461 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    32521 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    32581 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt
    32641 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    32701 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    32761 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    32821 agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac
    32881 aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa
    32941 actacgccgt cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc
    33001 gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc
    33061 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    33121 tttttgtgaa cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg
    33181 cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    33241 cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc
    33301 tggatcttgc ggacctgcgt ctggaatcgg ccatctgcct gttccaccag cgcttctcca
    33361 ctaacaccgt accgcgctgg ccgctggcgc agccgttccg ctatctggcg cataacggtg
    33421 aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga
    33481 cgccgcttat ccctgatctg cacgacgcag cgccattcgt caacgaaacc ggttctgact
    33541 ccagctcgat ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg
    33601 ctatgcgtct gttagtgccg cccgcctggc agaacaaccc ggatatggac ccggagctgc
    33661 gtgccttctt cgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg
    33721 tgatgtccga cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc
    33781 gctacgtcat caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    33841 atcagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg
    33901 acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    33961 atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    34021 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    34081 agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc
    34141 aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc
    34201 gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc
    34261 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct
    34321 tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact
    34381 ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata
    34441 tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag
    34501 ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta
    34561 aagatcgcct gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg
    34621 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc
    34681 accacttcgc cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg
    34741 aaacgctggg ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc
    34801 tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    34861 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg
    34921 tagtgggcct gtgcttccag ggggcggtca gccgcattgg tggggcgagc tttgaagact
    34981 tccagcagga tctgctgaac ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc
    35041 agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcttacaac ccggacgtgg
    35101 tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga
    35161 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    35221 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata
    35281 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    35341 tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg
    35401 gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc gttactccgg
    35461 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg
    35521 aaggcggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg cgctattcgg
    35581 tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact
    35641 tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc
    35701 tggtttccga accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt
    35761 taatcaccat cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat
    35821 acgcaggttg cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg
    35881 gtctgcgtca taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca
    35941 tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggcccg atggtggcgc
    36001 tcggctgtaa atatctacgt atttgccatc tgaacaactg cgcaacgggt gtagcaactc
    36061 aggatgacaa actgcgtaag aaccactatc acggcctgcc gttcaaggtt accaattact
    36121 ttgaatttat tgcccgtgaa acccgcgagc tgatggcaca gctgggcgta acgcgtctgg
    36181 tggatctgat tggtcgcacc gacctgctga aagaactgga cggtttcacc gccaaacagc
    36241 aaaaactggc gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct
    36301 actgcaccga aaacaatcca ccgtttgata acggcctgct gaacgcgcag ttgctgcaac
    36361 aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat attcgcaaca
    36421 ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccaaacgcac ggcgatcagg
    36481 ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg
    36541 tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta
    36601 aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc
    36661 atgaagcaag tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    36721 ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag
    36781 aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta
    36841 aaaccggcgt taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa
    36901 gcggtgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc
    36961 tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct
    37021 ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg
    37081 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag
    37141 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    37201 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg
    37261 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt
    37321 cgtgcggcaa cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc
    37381 tgaagctcgc caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca
    37441 ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    37501 ctctgaacga tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata
    37561 aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag
    37621 tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg
    37681 gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta
    37741 ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca
    37801 tgggtattga attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc
    37861 tgagtgatta cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc
    37921 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca
    37981 aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac
    38041 gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc
    38101 agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    38161 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac
    38221 tggggattga agtgaacggt aacggtaaag tcagcggcgt aaaaatggta cgtaccgaaa
    38281 tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata
    38341 tcgttccggc agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc
    38401 tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg
    38461 acaacgcctt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt
    38521 ccgatctggt ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact
    38581 ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat
    38641 tctcctttct ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca
    38701 tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc
    38761 atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa
    38821 tccacgacgg tattattcat ctggtcgata gcgcccggac ggaaacggta cgtagcgtta
    38881 acgcgctgat gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag
    38941 gtggcgaggc cagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa
    39001 gtctaaggta taagcgtggg ttctctgcaa aaaacttacg ccaaatgagg cttttttacc
    39061 tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa
    39121 ttccgcagac accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca
    39181 aatctttccc cctgccctgg tccacctacg tccgtttact ttccgttaaa aacgctgacg
    39241 cccgcagctt ttatgaaaaa gagacgctcc gctgtggctg gtctgttcgc cagctagaac
    39301 ggcaaattgc gacccaattt tatgagcgaa tactactgtc acatgacaaa tcagccatgc
    39361 tgcaacaaca cgctcctacc gagacgcata ttcttccgca acaggcgata cgcgatccct
    39421 ttgtgctcga atttctggaa ttgaaagatg aatattcaga atctgatttc gaggaggcgc
    39481 tgatcaacca cctgatggat ttcatgctgg aacttgggga tgattttgcc tttgttggtc
    39541 ggcagcgaag attacgcatt gatgacaact ggtttcgtgt cgacctgctg tttttccacc
    39601 gccgtttacg ctgcctacta atcgtcgatc taaaagtggg caaattcagc tatagcgatg
    39661 ccggacagat gaatatgtat ctcaactacg ccaaagagca ctggacgcta ccggatgaaa
    39721 atccgcccat cggtctggtt ctctgtgcag agaaaggagc cggagaagca cattatgctc
    39781 tggcaggttt gcctaacacc gttctggcaa gcgaatataa gatgcaacta cctgatgaga
    39841 aacgactcgc agatgaactc gttcgaacac aggcggtgct agaggaaggc tatagacgcc
    39901 gttaatttca gcggtttcga attctgcaga cagtgtctgc agaatcttta aaacgaacta
    39961 agttttaagc cattaaatca gccttaacct tcaccaccac ctttttaatc tcacccggct
    40021 cgccaacaac gcatcccggc ttatgcggct ctcctggcat aaacacggcg aacattcccg
    40081 gttttaagat gatggtttgc tcgttttcaa tggcgctgca aagctgataa tcatcctcat
    40141 ggtggaactc ttcacactga cgcgcggtgc ctgccatgcc aaacagaatc cgttcctcac
    40201 cgtttaataa cagctggata tcaatgtatt gctcgtgcaa ttccgctttt ttctcgacgg
    40261 gcgattgagt gttaaacgtc atgacattca taaagatatt gtcgccctgt aattcgtaac
    40321 gacccggcgc tttttcttgc ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg
    40381 caggatgtaa cccagcagac ggtaatgact gtacttcacc catcatcata atttttctcc
    40441 ctgggccaac agcgcagccc caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa
    40501 atcaacatga aatgccgctg gctcctgcgc cagatacgtt tccaccagcg ccagataccc
    40561 ttctgccagc ccaacgctgc caccgaccac cacgcactgg caatcagttg tggctttaat
    40621 atcagcgatc agccttgcca gcacatgtgc ggagcggtga atcagttgct gcgcctgctc
    40681 gtcgccctgc ccggcgcgtg tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg
    40741 cgctgccgcc gcaatgccgc gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca
    40801 gccgcagact gggccgtgtg gatcggcaag cgtatgcccg atatgccccg ccagaccgcc
    40861 agggccggta agcagtttac cgccgctcac tacaccgccg ccgacgccag tggaaacggt
    40921 gataaacacc atctcggtta catcgccttc cagcgcctga tactctgccc atgctgcggc
    40981 ctgcgcgtcg ttaatggcaa tggtcggcaa attggtaagt tgttccagcg ttttgactaa
    41041 cggaaagtgt agcaatccac cgagattatg cggattcagc gccagcaaac tgccgtcgcg
    41101 aataattccg gttgaagcga tggcaacccg ctgcgcatga gcttgcaacg gagagactaa
    41161 tgcggataag gcatcacgca aggcttctgg tgtctggctg gctggcgtag gaagttcacg
    41221 acgatcgcgg atctgcccgt cagcgccaat cagcgcggcg gcaagtttag taccgccgat
    41281 atcaatcgcc agtgtggtca tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa
    41341 atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc
    41401 gcatcagccg cctgagcagg cgtgttgtaa cgcccttcgg caatcacccg acatccggcg
    41461 tcgctcaacg ttttcaccag cgccagatcc ggctcttctg gcgtttcagg cgtggtatag
    41521 ccagaaagcg tagtgccaat aatttcggct cccagctttt ggcatgccag gccgtcttcc
    41581 ggcgttgagc agtcggtcat cgccagtaaa ccgtgatggt gaatacgtgc cagcagcgtt
    41641 tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc
    41701 tgcgccagcg catcgacatc ttcaatatag gccgtgatgc gtaccggaga atcctccaga
    41761 tcgcgtttca caatcccgat aattggcacg ctcaccaccg cacgcgtggc ttgcagattt
    41821 gccacacctt caatgcgaat ggcaaccgcg cccgcctgct ctgccgctaa tgccatggcg
    41881 gcgacgattt cgggtttatc gagcgggctg tccggaaccg gctggcagga gacaatcagg
    41941 ccaccgttag cagcgatttt ttgatccagt tgtgcaagta acgacataca tcttccctta
    42001 gcgaaaggcc cggtacatag accgggcaac aggattaact tttggttttg actaaatcgt
    42061 ttttggggct gccaaatggc acggcaccgc taaatggttt gccgtcgata gcgtcatgag
    42121 tacgcaatgc ttccgggcgc agccaacgct gaacgcggga aggcatatcc agaccaatca
    42181 gcaggatcac cacaaatgtc agactgaacg agagcgatgc cagcgcagta cccagatcca
    42241 gacgttgagc gatcaacgcg ccgatgattg gggccagtgc gccgcccaat gcgccaacgt
    42301 tgtaggtaaa gcccaggccc gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt
    42361 tcggtaagat cccggcgatc ccttgtccaa gcatttgctg gaagaacagt aacagaccga
    42421 gcacccagac gtttgcgccg ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca
    42481 gcaggctaca aacgtacgct ttgcgggttc ccagccagtc accgaggaag ccacctacgc
    42541 agcatcccac ggccgcgcca aagccactaa agaacagcac attggcgaca gtatgcgggt
    42601 tataagccag atcggttttc agatacgttg gcagcagcgc ctgaatcggc catgagtaga
    42661 ggaaagcaaa caacacgacc accatcagca ttacgcccgt tggccagcgt ttacccgtac
    42721 tttgcaccat aaagctgata aagattgcgg cgcataacag cccgagaaca gcgacgattg
    42781 cagcattttg caggttaccg gcgaagcaga accacagcgc agtagccgcc gccagtgtca
    42841 ttacgatatt ggcaatgcga tgttcaccac ggtagagaat atccaccatt gtgcgtactg
    42901 gtgctttacc tgcgtgtttc tctttccagt cttccgcttc cgggatgttt ttacgcagcc
    42961 agagagcaaa gatgattggc aaaatgccga taaagaacag cgcacgccaa ccccagaccg
    43021 gaaccaccag gctatagacc tgagcggcaa cgacggcccc cacagagaag cctgaaatca
    43081 aaaaaccact ggctttgtta cgcagatgtt ttggccagct ttcaatgaca taggtggcgc
    43141 tggaaccgta ttcacccgcc atccccatgc cgatgaccag acgagcgata aacatggtga
    43201 tgtagcctgg cgcaaagccg caggccagcg tcccggccga gaagagaacg atgctggtga
    43261 ccattgccag acgacgcccg tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc
    43321 agcgagagat aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc agcccgaatt
    43381 caccctgtac ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg tcaagcagat
    43441 atcccaacca ggcagcggaa aatgcccgcc attgtgcacg attgagatgg cgataccacg
    43501 ggatattctg ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg cccacacgct
    43561 ttggtatgaa aattgtaggg tacagatgcg tttatttccc ctcacccggt aggggcgagc
    43621 gaggggaaac aactcacccg cgctcttgca tcaactgctg ggccagcgcc ttcagttctg
    43681 gcagatattt ttcatctacc ggaccaaacg gtttgcggca cagcggcaca gaaacgacat
    43741 ccatataatg caggacagtt ttcaggccgc ggaatacgcc cgttttgatc agtaaatcaa
    43801 tgactttatt acattcggtt tgcagcttct gcgcggtctg gatatcgcct tctttcagcg
    43861 ccttcacaat cccctgataa cgccagccca tgatgttgta ggtactaccg ataccaccat
    43921 cagcgcccgc cagaagacca gaggcgaaga tttcgtcgta accgttatag agcaccagat
    43981 caggatgttc acgacggatc tgctccatct gatagagatc gccagaggtc tgtttcagcg
    44041 cacctacgcc aggcaacgta acaagtgtgt tgatctgatc cagggtcagt tttaccccac
    44101 tcagggctgg aatgttgtac accaccatcg gcaaaccatc cgccgaatca ataattgccc
    44161 gatagtgatc gcagtgttct tcaaagctga aaggatagta gaacggcgtg acggcggaga
    44221 cggcatcgaa gccataacgt ttagccgatg ccgcaagttg ttggctttcg gcggtgctga
    44281 cgcaaccgac gtgggcgatg agtttaatct gacctttcgc ctcttcggcg acgatttcca
    44341 gtacctgttc acgctcggaa aggctttgta caaaggcctc gccggtcgaa ccacccacgt
    44401 ataaaccgtc gatgccctgc tgaatattga actgaaccag gcgacgcaga ctcgctttat
    44461 ccagtgcttg ttgttggtca aaaggagtca ggagtgcagc cattacgcca cgtaaattcg
    44521 ttgccataaa tacctctgaa gtgatgcttg tctgataaac gatatacctt tatacctgtt
    44581 ataccagatc aattaagcaa caccccatac agaaagctta taatgcgatc tgcttcacta
    44641 aagtggcatt atttcttttt gttggtggtc tgaccgaaag cgtgccaggt agcagagacg
    44701 ctgttgagat gcgattgcaa cgcacgatcg gcttcgtcag gatcatgacg gcggatcgca
    44761 tcaacgatcg caatatgctg ttgataacta acgttgttat gttcgtgcag tgcctgatcg
    44821 gtaaccgttg ggcgtgcggc aataagccag tcgagcaggg caacgtggat cgccatgaag
    44881 attgggttac cggggatctc cgccagcacg cggtggaaat caacgtctga acgaatgaat
    44941 gccgcgttgt tatccagcga ctgactgttg atttccagtg cttttgccag caaatcgatt
    45001 tgctcatcgg tggcatgttc agccgcatag cgcaccaggc tggattcaaa gaacagacgt
    45061 aattgttcga aatgggcaat cccaccagga tgagaaagga aatctttcgc catgccggaa
    45121 agctcgccga tgatggtgtc cgcagaagga cgtgagacgc gggcacgttc gccgttgttg
    45181 atttgcacca gacctttgcg ttttaacgct gccagcgctt cgcgcacaga aggacgcccg
    45241 acgttaaaga acgccatcag ttcgcgttca gacggtaatt gttcaccttc gccaaattca
    45301 cgacggcgga tcatctgttc cagctcttct tccaccattt cggagagttt tttacgcgcc
    45361 agcgggcggc tacgcaagtt gcgaccaatt gcaggtgaag aatcttcggt ttgcgaatca
    45421 aatgcgttca taaggcccat tctgtaaggt cagtgtgatt aacatcatca gtgacatcct
    45481 atcacaggat tgaaagtagg ggaaaatggc aggtttttct ctttgtgcct catcattacc
    45541 ataattaacg gaataattaa ctattgcgaa aaattaatgt aacgcagata aaaacatccc
    45601 gtttgaatta tttataagac tattcacgag cattatgaat attatgaatg tgttcttaca
    45661 aaataatcat aagcgcatat tttttaatga aaaatcacct cacctacaat taaaaacacg
    45721 acatccgcac cataaataga cttgcaaaaa atataacatc attgttttca atctgccgtt
    45781 tatgggattg accgttttct tttgacacgg agttcaacaa tgttcggcat aattatatct
    45841 gtcatcgtat taattacgat gggctatttg atcctgaaaa actacaaacc tcaggtgctg
    45901 ctggctgccg caggtatctt cctgatgatg tgcggtgtct ggttagggtt cggtggtgta
    45961 ctcgatcccg ccaaaagcag cggctacttg atcgtcgata tttataatga aatcctgcgc
    46021 atgctgtcca accgcattgc cggattgggg ctgtcgatta tggcggtggg cggttatgcc
    46081 cgctacatgg agcgcacagg agccagtcgc gcgatggtga gcttgctaag ccgcccgtta
    46141 aaactcattc gctcgccgta tattattctg tcggcaactt acgtcatcgg ccaaatcatg
    46201 gcgcagttta ttaccagcgc ctccggcctg ggtatgttgc tgatggtcac cttatttccg
    46261 acgctggtga gtctgggagt aagtcgcctc tctgcggtgg cggttatcgc aaccacgatg
    46321 tccattgagt gggggattct ggaaacgaac tccatttttg cagcccaggt cgcgggaatg
    46381 aaaattgcca cttacttctt ccactaccag cttccggtcg cctcttgcgt cattatctcg
    46441 gtggcgatct cccacttttt cgtgcaacgc gcttttgaca aaaaagataa aaatatcaat
    46501 cacgaacagg cagagctaaa agctctcgat aatgtcccgc cgctctatta cgccatttta
    46561 cctgtgatgc cgttaatctt gatgctcggc tcgctgttcc tcgcccacat cgggctgatg
    46621 cagtcagaac tgcatctggt ggtggtgatg ttactgagtt tgacggtgac gatgtttgtt
    46681 gagttcttcc gcaagcataa cttgcgcgaa acaatggacg atgtgcaagc gttttttgac
    46741 ggcatgggta cgcagtttgc caacgtggta acgctggtgg tcgcgggtga aatatttgcg
    46801 aaaggcttaa cgacgattgg cactgttgat gcggttatca ggggtgcgga gcattctggt
    46861 ctgggcggta ttggcgtgat gattattatg gcgctagtca ttgccatttg tgccattgtg
    46921 atgggctctg gcaatgcgcc gtttatgtca tttgccagtc ttattccgaa tatcgcagcc
    46981 ggattacatg taccagcggt tgtaatgatt atgccgatgc attttgccac gacgctagcg
    47041 cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt caggaattgc aggcgtttcg
    47101 ccttttgcgg tggtgaagcg aacagcgatc cccatggcag tcggtttcgt ggtgaatatg
    47161 attgccacaa tcacgctatt ttattaagcc attaaaagac aaaacaggcc accagggcct
    47221 gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggagg
    47281 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
    47341 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta
    47401 ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac
    47461 ggcagccagc ggcacagaaa cctgacgcgg aatgccgcca aagcgcgcgt taaagcgtac
    47521 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
    47581 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccaa
    47641 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
    47701 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
    47761 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
    47821 ttcagctctt tcgcacccgg gccgctgaac tcgatgccca gttgcggcag acgccacagc
    47881 agcggagcaa gatagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
    47941 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
    48001 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
    48061 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
    48121 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
    48181 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
    48241 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
    48301 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
    48361 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
    48421 ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga aaatcaaaca
    48481 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
    48541 gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
    48601 agttgactta gtcccttatt ggcgatgtgg tttttgtttt gcctgtctgt cagacggtag
    48661 caaaaagcaa ctttccaatt tttacgctga ttcagaattt aaccataaaa aaacccgccg
    48721 aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
    48781 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
    48841 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
    48901 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
    48961 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
    49021 catgcgagca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
    49081 accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta
    49141 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
    49201 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
    49261 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
    49321 agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata
    49381 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
    49441 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg
    49501 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
    49561 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
    49621 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
    49681 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
    49741 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
    49801 tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
    49861 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg atcgccctga
    49921 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
    49981 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
    50041 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
    50101 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
    50161 gtcgccaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cgcccccgcc
    50221 agcgccaacc atagtttatc catctgcgcc cgggtttcat cgttgagtgg cgaaagccac
    50281 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
    50341 tcgcaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caggccattt
    50401 cgataaagtt catccggcgg aatatttgac gtcgctacca gggtaatgcc gcgggcgaac
    50461 agggctttca tcagaccgcc aagtagcatg gcgtcggtaa tatcagaaac aaaaaattcg
    50521 tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg
    50581 gtctgcccct gtaaggcagt tagctcttcg tgcacccgca gcataaaacg gtgaaagtgc
    50641 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
    50701 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
    50761 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
    50821 ctgctattga tgagttcttg ataaataatt tccaggcggc tgacggcctc tttttgaacg
    50881 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
    50941 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
    51001 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
    51061 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
    51121 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
    51181 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
    51241 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
    51301 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
    51361 tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac
    51421 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
    51481 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
    51541 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
    51601 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
    51661 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt
    51721 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
    51781 ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg
    51841 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
    51901 gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa
    51961 ggtttaggtt ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
    52021 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
    52081 aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca aaacccgagc
    52141 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgta
    52201 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
    52261 ggccgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
    52321 aaccgcggta actccggcgg tgcactatta aaccttaacg gtgagttaat tggcatcaac
    52381 actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat ccccagtaat
    52441 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
    52501 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
    52561 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcaaaagc gggcgtcaaa
    52621 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
    52681 tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc
    52741 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
    52801 atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
    52861 aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
    52921 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
    52981 aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
    53041 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg
    53101 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
    53161 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
    53221 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
    53281 ctgccagtta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
    53341 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccttggga tccggtgtaa
    53401 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
    53461 tcatcgtcgc cttacaggat ggtcgtgtat ttgaagcatt gctggtggga tctgactctc
    53521 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccgatca
    53581 atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac ccgtacaacc
    53641 tcgggcagac cattacccag gggattatta gtgccacggg tcgaatcggt ctgaacccga
    53701 ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac tctggcggcg
    53761 cgctggtgaa ctcgctggga gaactgatgg gcattaacac gctgtcgttt gataagagta
    53821 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
    53881 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt atcggcggac
    53941 gcgagatcgc accactgcac gcgcagagcg gtggtataga tcaactgcaa gggatcgtgg
    54001 ttaatgaagt gtcacctgac ggcccagcgg cgaatgcggg tattcaggtc aacgatctga
    54061 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg
    54121 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
    54181 tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaac
    54241 cggagtctgt gctccggttt tttattattc gctaatcaat tacttattaa cgaactcttc
    54301 gcccagggcg atatctttct tcagcgtatc cagcatacct tccagcgcgt tctgttcaaa
    54361 tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca gcagcagcgg
    54421 ttgagagaag aaacgggcgt actgaccgtc gccttcaacg taggcacatt cgacaacgcc
    54481 ttgttcgccc tgcagtgcac gaaccagaga cagaccaaaa cgtgcagctg cctggcccat
    54541 agacagggtt gcagacccgc caccggcttt cgcttcaacc acctcagtac ccgcgttctg
    54601 gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga
    54661 cagcagcggc agaatggtaa caccagagtg accgccaata accggcactt caacttcgcc
    54721 tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat ccagcgtggt
    54781 aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt cagcagcaat
    54841 tgcaacagtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg ggcaggtttt
    54901 cgcaacttgc tgtaccaggt ttttcacgat gccggcgtta acgttaaaca ggtcggaacg
    54961 atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg cgccttccag
    55021 cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag ggatatggct
    55081 cagatcgacg gccacaccgg gagtcactgg agcgatatca tacagagaga gttctgaacc
    55141 tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc
    55201 gaggactgcg actttcatcc taaactcctt attatattga taaactaaga tatgttgctc
    55261 cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct caagaatgtg
    55321 tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc taacttacgt
    55381 aattaacagt cgcttatgca ttagcgcaac attccaacag gtggtgacaa tataccctac
    55441 cgttcagcca aaacaacatc aatttgataa caattaattt acttttaagc agaatttgca
    55501 tgccgtgacg caggcatgtt tctcaataac gaaatttgat aaaatcccgc tctttcataa
    55561 cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg tatgcacaat
    55621 aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag caagaagaac
    55681 tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag ggcgaaatcg
    55741 tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc tcgcggatgt
    55801 tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt tactgcctgc
    55861 cagctgaact gggtgtacca actacctcca gtccattgaa gaatctggtg ctggatatcg
    55921 actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag ttaattgctc
    55981 gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct ggcgatgaca
    56041 ccatctttac cacccctgct aacggtttca ccgtcaaaga cctgtacgaa gcaattttag
    56101 agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt
    56161 gcttatccct ctcattcccc ttctttctcc tgcgatacat ttaacgaata gtgcgtttta
    56221 ccgcgacaca tcattcacaa aatgaataca tacggtaaaa aaagcacatt atgcaaaatt
    56281 cattatctaa ttgaaaatac tataattaat aataaata
//
LOCUS       DAFAVF010000014        54106 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFAVF010000014 DAFAVF010000000
VERSION     DAFAVF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.014"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(273..1297)
                     /locus_tag="LGK42_000815"
                     /pseudo
     CDS             complement(273..1297)
                     /locus_tag="LGK42_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1420..1872)
                     /gene="tabA"
                     /locus_tag="LGK42_000816"
     CDS             complement(1420..1872)
                     /gene="tabA"
                     /locus_tag="LGK42_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBK2657094.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2017..2610)
                     /locus_tag="LGK42_000817"
     CDS             complement(2017..2610)
                     /locus_tag="LGK42_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK2657095.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCTTAALAGEAIKTILKE"
     gene            2681..3394
                     /gene="bdcA"
                     /locus_tag="LGK42_000818"
     CDS             2681..3394
                     /gene="bdcA"
                     /locus_tag="LGK42_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2657096.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3525..3920
                     /locus_tag="LGK42_000819"
     CDS             3525..3920
                     /locus_tag="LGK42_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBK2657097.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMMVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            4201..4335
                     /gene="pyrL"
                     /locus_tag="LGK42_000820"
     CDS             4201..4335
                     /gene="pyrL"
                     /locus_tag="LGK42_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBK2657098.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4339..5274
                     /gene="pyrB"
                     /locus_tag="LGK42_000821"
     CDS             4339..5274
                     /gene="pyrB"
                     /locus_tag="LGK42_000821"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBK2657099.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5287..5748
                     /gene="pyrI"
                     /locus_tag="LGK42_000822"
     CDS             5287..5748
                     /gene="pyrI"
                     /locus_tag="LGK42_000822"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBK2657100.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5821..6207
                     /gene="ridA"
                     /locus_tag="LGK42_000823"
     CDS             5821..6207
                     /gene="ridA"
                     /locus_tag="LGK42_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBK2657101.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6413..9109)
                     /gene="mgtA"
                     /locus_tag="LGK42_000824"
     CDS             complement(6413..9109)
                     /gene="mgtA"
                     /locus_tag="LGK42_000824"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK2657102.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9250..9303)
                     /gene="mgtL"
                     /locus_tag="LGK42_000825"
     CDS             complement(9250..9303)
                     /gene="mgtL"
                     /locus_tag="LGK42_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBK2657103.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9488..10435
                     /gene="treR"
                     /locus_tag="LGK42_000826"
     CDS             9488..10435
                     /gene="treR"
                     /locus_tag="LGK42_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBK2657104.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10554..11975
                     /gene="treB"
                     /locus_tag="LGK42_000827"
     CDS             10554..11975
                     /gene="treB"
                     /locus_tag="LGK42_000827"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBK2657105.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12024..13679
                     /gene="treC"
                     /locus_tag="LGK42_000828"
     CDS             12024..13679
                     /gene="treC"
                     /locus_tag="LGK42_000828"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK2657106.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            14073..16211
                     /gene="nrdD"
                     /locus_tag="LGK42_000829"
     CDS             14073..16211
                     /gene="nrdD"
                     /locus_tag="LGK42_000829"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK2657107.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16369..16833
                     /gene="nrdG"
                     /locus_tag="LGK42_000830"
     CDS             16369..16833
                     /gene="nrdG"
                     /locus_tag="LGK42_000830"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBK2657108.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16878..17264)
                     /gene="cybC"
                     /locus_tag="LGK42_000831"
     CDS             complement(16878..17264)
                     /gene="cybC"
                     /locus_tag="LGK42_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBK2657109.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17447..18799)
                     /gene="pmbA"
                     /locus_tag="LGK42_000832"
     CDS             complement(17447..18799)
                     /gene="pmbA"
                     /locus_tag="LGK42_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBK2657110.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18893..19444
                     /locus_tag="LGK42_000833"
     CDS             18893..19444
                     /locus_tag="LGK42_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBK2657111.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19600..20973)
                     /gene="mpl"
                     /locus_tag="LGK42_000834"
     CDS             complement(19600..20973)
                     /gene="mpl"
                     /locus_tag="LGK42_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBK2657112.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21149..22147
                     /gene="fbp"
                     /locus_tag="LGK42_000835"
     CDS             21149..22147
                     /gene="fbp"
                     /locus_tag="LGK42_000835"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBK2657113.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22180..23175)
                     /gene="yjfF"
                     /locus_tag="LGK42_000836"
     CDS             complement(22180..23175)
                     /gene="yjfF"
                     /locus_tag="LGK42_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBK2657114.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23162..24184)
                     /locus_tag="LGK42_000837"
     CDS             complement(23162..24184)
                     /locus_tag="LGK42_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2657115.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24198..25700)
                     /locus_tag="LGK42_000838"
     CDS             complement(24198..25700)
                     /locus_tag="LGK42_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2657116.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25840..26796)
                     /gene="ytfQ"
                     /locus_tag="LGK42_000839"
     CDS             complement(25840..26796)
                     /gene="ytfQ"
                     /locus_tag="LGK42_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBK2657117.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27106..27636
                     /gene="ppa"
                     /locus_tag="LGK42_000840"
     CDS             27106..27636
                     /gene="ppa"
                     /locus_tag="LGK42_000840"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBK2657118.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28016..28357)
                     /locus_tag="LGK42_000841"
     CDS             complement(28016..28357)
                     /locus_tag="LGK42_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBK2657119.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28360..32139)
                     /gene="tamB"
                     /locus_tag="LGK42_000842"
     CDS             complement(28360..32139)
                     /gene="tamB"
                     /locus_tag="LGK42_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBK2657120.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32136..33869)
                     /gene="tamA"
                     /locus_tag="LGK42_000843"
     CDS             complement(32136..33869)
                     /gene="tamA"
                     /locus_tag="LGK42_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBK2657121.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34075..34713
                     /gene="msrA"
                     /locus_tag="LGK42_000844"
     CDS             34075..34713
                     /gene="msrA"
                     /locus_tag="LGK42_000844"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK2657122.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35036..36379
                     /locus_tag="LGK42_000845"
     CDS             35036..36379
                     /locus_tag="LGK42_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK2657123.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36441..36647)
                     /locus_tag="LGK42_000846"
     CDS             complement(36441..36647)
                     /locus_tag="LGK42_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBK2657124.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36972..37529
                     /locus_tag="LGK42_000847"
     CDS             36972..37529
                     /locus_tag="LGK42_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBK2657125.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37519..38259)
                     /gene="cysQ"
                     /locus_tag="LGK42_000848"
     CDS             complement(37519..38259)
                     /gene="cysQ"
                     /locus_tag="LGK42_000848"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBK2657126.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNIMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38449..40392
                     /gene="cpdB"
                     /locus_tag="LGK42_000849"
     CDS             38449..40392
                     /gene="cpdB"
                     /locus_tag="LGK42_000849"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBK2657127.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYISAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNNSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPDNTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40515..40895)
                     /locus_tag="LGK42_000850"
     CDS             complement(40515..40895)
                     /locus_tag="LGK42_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK2657128.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40984..41844
                     /gene="qorB"
                     /locus_tag="LGK42_000851"
     CDS             40984..41844
                     /gene="qorB"
                     /locus_tag="LGK42_000851"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBK2657129.1"
                     /translation="MIAITGATGQLGHYVIKSLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            41953..42918
                     /locus_tag="LGK42_000852"
     CDS             41953..42918
                     /locus_tag="LGK42_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2657130.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43026..43688
                     /gene="ytfE"
                     /locus_tag="LGK42_000853"
     CDS             43026..43688
                     /gene="ytfE"
                     /locus_tag="LGK42_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBK2657131.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43733..45145)
                     /gene="cycA"
                     /locus_tag="LGK42_000854"
                     /gene_synonym="dagA"
     CDS             complement(43733..45145)
                     /gene="cycA"
                     /locus_tag="LGK42_000854"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBK2657132.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45454..46074)
                     /gene="fklB"
                     /locus_tag="LGK42_000855"
     CDS             complement(45454..46074)
                     /gene="fklB"
                     /locus_tag="LGK42_000855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2657133.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46293..46931
                     /gene="ytfB"
                     /locus_tag="LGK42_000856"
     CDS             46293..46931
                     /gene="ytfB"
                     /locus_tag="LGK42_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBK2657134.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46915..47562)
                     /locus_tag="LGK42_000857"
     CDS             complement(46915..47562)
                     /locus_tag="LGK42_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK2657135.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYITNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47775..48566
                     /locus_tag="LGK42_000858"
     CDS             47775..48566
                     /locus_tag="LGK42_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBK2657136.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            48576..49418
                     /locus_tag="LGK42_000859"
     CDS             48576..49418
                     /locus_tag="LGK42_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK2657137.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPQGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPELASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            49428..50204
                     /locus_tag="LGK42_000860"
     CDS             49428..50204
                     /locus_tag="LGK42_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBK2657138.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            50214..51755
                     /locus_tag="LGK42_000861"
     CDS             50214..51755
                     /locus_tag="LGK42_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBK2657139.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            51752..53824
                     /locus_tag="LGK42_000862"
                     /pseudo
     CDS             51752..53824
                     /locus_tag="LGK42_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            53917..>54106
                     /locus_tag="LGK42_000863"
     CDS             53917..>54106
                     /locus_tag="LGK42_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657140.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQP"
BASE COUNT        13338 a        14228 c        13542 g        12998 t
ORIGIN      
        1 aattgataga aataccactt agcaaaatca ggttgcttca tgaaaaaatc cttcaaaaaa
       61 atatcattca ctaaaatact gagaggcact ttatcaaaaa ccaatttaac atcatcaaat
      121 agccttagta ttaagcgcga tttttcttga ttacttaaat taaaatcatt gacatataca
      181 accaacttct cccaaatcaa ttcctgtaac tcagggctgt ttttataggg ttctttattg
      241 aggttgaaaa ataatgtctc agctatttta gactaatcgc agttaaaatc aatatgatcg
      301 atcacggagc gggcatgctt tatctttgta gtatcatcag aatattgcat actttgatta
      361 aaacaccact ccagaatttt tacatcatta atatatggca attttaaaaa agtcttcaac
      421 atcgaatcga tgaccttttc gagattaact tttgcatcat ctaaggaatc aataagaata
      481 ttagacaggt taattttgat gttattaatt tcatcattac cttctgtacc actagcaatt
      541 ctggtatcat gtaacgtttt cagatcattt atttcttcaa gtataccacc ttcgcctagt
      601 aagtatggaa tcgtgtcatc acatacatga ggcattaata gcagatgttc tttctttatt
      661 tttctaatgt actggctaat ggcttttcta ataactcgtt ataatcaatt gcgtccttcc
      721 agttcaactg atgattattt aatagaagcg ccatattctt gttagcttca cgttggtaac
      781 acgccagaac cttgttagta atcaggtgta atgaactatc atcttttctt tcaaaagact
      841 taccttcata acgaaaactc aaaaaatcag attgtggaaa tataatggct gtatgtcctt
      901 gacgagtccc catagtagtg gtagtagaaa gtgaaatttc tataatcacc ttagatgaat
      961 ccgcacgctc accttcagga ttattcataa caaaattcat tatttcactg ccatcattat
     1021 ttttgatata agatgaggac tcaatatgag ccatcatatt tctacccgct tcgttggagc
     1081 gtttgctaaa atccacataa cccagggtaa atatttttgc caaaaactcc aaaaatgctc
     1141 caggagatag aatattcctt ctgctatctt gcccctcggc attacgagca aaatcattca
     1201 ttcccacaca ccaggtattt aagacttcat tttttggttt aacattgtcg gctgatgcca
     1261 tgtgttgata acaattcaaa tctgatattt ttgccatgac gaactccttg agttaataag
     1321 aacgtctaag cattacaaat tttactcaca aaaaaatatc aaaaatcgct cttttcacaa
     1381 actcgccgta aacctggagt tatttacggc gaaaagtcat cacgccatca gcatcttcac
     1441 tactgctttg cgaacctggg ctggtgcacc cactgcgcac agcggtttat gcacttcccc
     1501 cggataaaac acaacaaaat caccttcatt caggataact gttttctcat caacgacttc
     1561 cggcaaaaat gcgatgtctt tatctactaa ccagtcggtc tccggcgtac ctgcaggttg
     1621 tgtgctgaag gtcatgcctt cctgaccttt taacacaatc tgaatgtcga gataacgggc
     1681 atggtactcc gcacggcgag ctttgtacgg ctcggtcata tcttccgaga taagataaaa
     1741 cagacgattg ccttcgatat cgtgcttacc ctttggcgtt tctgccgtaa cgtgtgcttt
     1801 gatatgctca atcgcctggc gtaactcctg gggtagccat ggctgaagat tatgaatatt
     1861 tccgatgatc atcttttcca ctccttaatt aaaacaatgt ttcatttcca ccgttatacg
     1921 aaaagatgat caattccgcc agccttaacg cgtgaattat tgtcttatca caacttatcg
     1981 gcaggcgcgt tcccgccttt cggatcaagg tcagcatcac tccttgagaa tagtttttat
     2041 cgcttccccc gccagtgcag ctgttgtaca gagttgttcg agggagtgcc cctctcgtgc
     2101 cttcgcagaa agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg
     2161 tggatgccgc ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaac
     2221 ggcaatatca cgcgcttgtg gatcatgact atgaatgcct tcaagaacca tacagccagc
     2281 gcagccgccg ttttggctat atctgcgcgc cgcttctttt aatacctcag ccaggcactc
     2341 ccctaccgga cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacata
     2401 ttcattgagt acacggctaa ataacccagc cttactgcca aaagccgcgt agagactcgg
     2461 ggggttaata ccaagataat cagtaacttc agcaacactg acagcatcga aacctttttg
     2521 gtgaaaaagc acttttgccg cagagactgc ctgctcagga gcgaaacgtc tgggacgacc
     2581 tggaacgcga gattgttttt tagtgaccat tacaaaactt gttgacagaa agttaaaaca
     2641 gttttgtaat gcatgttaca taataaatca aggagtcctt atgggcgctt ttacaggtaa
     2701 gacagttctc atcctcggtg gtagccgtgg aataggtgcc gctatcgtac gtcgtttcgt
     2761 caccgatgga gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctgaacgcct
     2821 ggcacatgag actggagcga cagcagtatt cacagatagt gctgacagag acgctgttat
     2881 tgatgtcgtt cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt
     2941 ctttggcgat gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat
     3001 tcatgcccct taccatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat
     3061 cttaatcatc ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc
     3121 cgccagtaaa tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg
     3181 tgggatcacc atcaacgtcg tccagccagg gccaatagat accgacgcta atcccgccaa
     3241 cgggccaatg cgcgatatgt tgcatagttt tatggctatc aaaagacatg ggcaaccgga
     3301 agaggtcgct ggaatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc
     3361 gatgcatacc attgatggcg cgtttggggc ataaccgact acgctcaatt aagcccagcc
     3421 attatccatg atgtctgggt tttgtttact cacgtcgttc cctaaaagcg gctcccggta
     3481 aatataaatc ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta gaaagaaccg
     3541 ctgttttccc tgctggccga cattcactgt atgctgaaca tcgttattcg gcggctattc
     3601 gttccggcga tttgctgttt gtttccgggc aagtaggaag tcgagaggac ggaacaccag
     3661 aacccgattt tcagcaacaa gtcagactgg catttgataa tttgcatgcg accctggcag
     3721 ctgcgggatg cacttttgac gatatcattg atgttacgag ctttcatacc gatccggaaa
     3781 aacaatttga agacatcatg atggtcaaaa atgaaatatt tagcgcccct ccttatccaa
     3841 actggacggc ggtgggcgtt acctggctgg caggctttga ttttgaaatt aaagtgatag
     3901 cgcgcatccc tgagctgtaa gcaatagtgt tagccgttcg ttttcacact ccgccgtata
     3961 agtcggatga atggaataaa atgcatatct gattgcgtga aagtgaaaaa gaaaaaagca
     4021 ggaaatgtct gcaattattg ataccgaacg acagttctct tccagaatca aagcaaataa
     4081 aaatgcatac attttgactt ttaattcaaa taaaccgttt gcgctgacaa aatattgcat
     4141 caaatgcttg caccgcttct ggctatgagt ataatgccgg acaatttgcc gggaggatgt
     4201 atggttcagt gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc tggcctgccg
     4261 tttttcttcc cgttgatcac ccattcccag cccctcaatc gaggggcttt tttttgccca
     4321 ggcgtcagga gataaaagat ggctaatccg ctatatcaga aacatatcat ttccataaac
     4381 gaccttagtc gcgatgacct taatctggtg ctggcgacag cggcgaaact gaaagcaaac
     4441 ccgcaaccag agctgttgaa gcacaaagtc attgccagct gtttcttcga agcctctacc
     4501 cgtacccgcc tctctttcga aacatctatg caccgcctgg gtgccagcgt ggtgggcttc
     4561 tccgacagcg ccaatacatc actgggtaaa aagggcgaaa cgctggccga taccatttcg
     4621 gttatcagca cttacgtcga tgcgatagtg atgcgtcatc cgcaggaagg tgcggcgcgc
     4681 ctggccaccg agttttccgg caatgtaccg gtactgaatg ccggtgatgg ctccaaccaa
     4741 catccgacgc aaaccttgct ggacttattc actattcagg aaacccaggg gcgtctggac
     4801 aatctccacg tcgcaatggt tggtgacctg aaatatggcc gcaccgttca ctccctgact
     4861 caggcgttag cgaagttcga cggcaaccgt ttttacttca tcgcgccgga cgcgctggca
     4921 atgccgcaat acattctgga tatgctcgat gaaaaaggga tcgcatggag tctgcacagc
     4981 tctattgaag aagtgatggc ggaagtagac atcctgtaca tgacccgcgt gcaaaaagag
     5041 cgtctggacc cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg cgccagcgat
     5101 ctccacaacg ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt tgatgagatt
     5161 gcgacggatg ttgataaaac gccacacgcc tggtacttcc agcaggcagg caacgggatt
     5221 ttcgctcgcc aggcgttact ggcactggtt ctgaatcgcg atctggtact gtaaggggaa
     5281 atagagatga cacacgataa taaattgcag gttgaagcta ttaaacgcgg cacggtaatt
     5341 gaccatatcc ccgcccagat cggttttaag ctgttgagtc tgttcaagct gaccgaaacg
     5401 gatcagcgca tcaccatcgg tctgaacttg ccttctggcg agatgggccg caaagatctg
     5461 atcaaaatcg aaaatacctt tttgagtgaa gatcaggtag atcaactggc attgtatgcg
     5521 ccgcaagcca cggttaaccg tatcgacaac tatgaagtgg tgggtaaatc gcgccccagt
     5581 ctgccagagc gcatcgacaa cgtgctggtc tgcccgaaca gcaactgtat cagccatgcc
     5641 gaaccggttt cttccagctt tgccgtgcga aaacgcgcca atgacatcgc gctcaaatgc
     5701 aaatactgtg aaaaagagtt ttcccataat gtggtgctgg ccaattaatt gcggttggta
     5761 ataaaagtct ggctccctat aatgagccag actttttacc gctgtaataa aggagaaatc
     5821 atgagcaaaa ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta cgtacagggc
     5881 gttgatctgg gcaatatgat catcacctcc ggtcagatcc cggtaaatcc gaaaacgggt
     5941 gaagtaccgg cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt aaaagcgatt
     6001 gtcgaagccg ctggcctgaa agtgggcgat atcgttaaaa ccaccgtgtt tgtgaaagat
     6061 ctgaacgact tcgctaccgt taatgctacc tacgaagcct tcttcaccga acacaacgcc
     6121 accttcccgg cacgttcttg cgttgaagtc gcccgtctgc cgaaagacgt gaagattgag
     6181 atcgaagcga tcgctgttcg tcgctaatct tgatggaaat ccgggctatc atgcccggat
     6241 taagtctgat gacaaacgca aaatcgcctg atgcgctacg cttatcaggc ctacgtgatt
     6301 cctgcaattt attgaatttg ttggccggat aaggcattta cgccgcatcc ggcatgaaca
     6361 aagcttactt tgtctgcaat ctgaatcggg gctatcgtgc ccagtttatt ctttattgcc
     6421 agccgtaacg acggctatag aaccctttca ccaactgggt taatgtcata taccctgcca
     6481 gaatcgcaac cagccacggg aaatagctta acggcagcgc ctgtaattgc agataactgg
     6541 ccagcggtga aaacggcaat gcgatcccga caatcatcac gatcacggtc atgatcatta
     6601 acggccacga tgcacagctc tgaataaacg gcacacggcg ggtgcggatc atatgcacaa
     6661 tcagcgtttg cgacagtaag cccaccacaa accatcccga ctggaacagc gtttgcgttt
     6721 ccggcgtgtt ggcatggaat acccaccaca tcaggcaaaa cgtcagaata tcgaagatcg
     6781 agctgatcgg tccgaagaag atcataaagc gccccagatc cgccggattc caacgctgcg
     6841 gcttttgaat ttgctcgtcg tcgacgttat caaacgggat cgccacctgt gacacatcat
     6901 acagcaggtt ctgaataagc aagtgtaacg gcaacatcgg caggaagggc aagaaagcac
     6961 tcgccaccag cacgctgaac acattaccga agttggagct cgccgtcatt ttgatgtatt
     7021 tcagcatgtt ggcgaaagtg cgacgtcctt caataacccc ctcttccagc accatcaggc
     7081 ttttttccag caggatgata tcagccgctt cacgggcaat atctaccgcg ccgtccacag
     7141 aaatgccgat atccgcagcg cgtaaggcgg gcgcatcatt aataccatcg cccataaagc
     7201 caaccacatg cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc atcggcgtca
     7261 gacgagcaaa cagcgtggtg cgctgtgcga gatttgccag ttcgtcgtca gatagcgttt
     7321 caatatcact accaatgacc acctctcccg cgtccagccc cacttcatgg cacactttcg
     7381 ctgcaactaa ctcactgtcg ccagtaagga tttttacggt aattccactc gcttttaatg
     7441 ccttcagtgc cggagcagtt gtctcttttg gcggatcaag gaaagcaata tatccttcga
     7501 ggatcaggtc ggattcatcc gcccgctggt aatctccttc acgcgctggc aggtatttcg
     7561 tcgccaccgc aaccacgcgc agcccctgac gattcagcgt atcagtaacc cgcttaatct
     7621 tacgcagcat gatgtcatcg agcggcacaa tctcgccatt gtgacgcacc tgcgaacaca
     7681 cattgaggat ttcctgcaat gcacctttgc aaaccagctg atggtgctcg gtattttctg
     7741 ccactaccac cgacatccga cgacgctcga aatcaaacgg aatctcatca attttttgcc
     7801 aacgactggc cagcgagcgc gctgactctt catccgtacc ttcgagcacc gctgtatcga
     7861 gcaggttttt aagtccggtc tgataatgac tgttcaacca cgcgctatgc agcacgcgtt
     7921 cgctggtttt accggagata tcggtatgat tctccagcac aattttgtcc tgtgtcaggg
     7981 tgccagtttt atcagtgcac agaatatcca ttgcgccaaa gttctgaata gcatccagat
     8041 gtttgacgat cactttctgt ttcgacagct ttactgcgcc gcgcgccagc gtcgaggtaa
     8101 caatcatcgg caacatttcc ggcgttaagc ctaccgcgac cgaaagcgca aacagtgccg
     8161 cttcccacca gtcgcctttg gtgtaaccgt tgataagcaa caccaccgga gccatcacca
     8221 gcataaagcg aatcagcagc atactgacgc ggctgatccc ctgctgaaag gcattcggct
     8281 cgctttcctg ctcactaaca cgccccgcca gttgaccaaa ccaggtattg gctcctgtag
     8341 caatcaccat tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa cacagggtgt
     8401 cacactccag cggattgctg tgctccggct ggcgagtggt agcggctttt tccacgggca
     8461 gagactcacc ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc aagatacgta
     8521 aatctgccgg gatcatatcc cccgccgcca gttttataat atcgccgggc accagctggt
     8581 cgatcgggat ctccagccag ccattttcgc ctttgtcgtt aattacgcgc agcaccgtcg
     8641 cggtattgct gaccatcgct ttcagggcat ctgccgcttt agtggaacgt gcttcctgaa
     8701 taaagttcag caacgtagaa atagcgacca ttagcgcgat aacgcccgcg gcaaataaat
     8761 cttccgtggc gtaagaaata gcgccgagaa tggtgagtaa aatattaaat gggttgcgat
     8821 agcagaccca taaatgtacc caccacggcg acggttgttg tgcgggtaat ttattttcac
     8881 catgttgttc gcgggcagat tccacttccg cctgatttaa cccttccgga tgggtgtcga
     8941 acgttttcca caattcttct tcgggcatca ccgccatttt caggcaatgc gcacttaagg
     9001 acggcgggac caccgtattc actgtctgct gcgcgccagg caatggatca cgatgaacca
     9061 gacgggaagg taaatggcga atgagccggg taaagatttc tttaaacata aggagtccct
     9121 ccgcactgtc tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt acctgaaggt
     9181 aataaagctg agcagggacg ttaaaacgtc acggccttac gcatccggta acgccgtgaa
     9241 aagacgggct taccggaaaa gttttaatct ccgtcgaggg agaggcgtgg gatcaggttc
     9301 catataacct ccggtaagtg aataaagcgc cgcaaataat aaggagaatc caataatatt
     9361 cgtagcatct atggcgatat aatacctgct ggcaactaaa ccaacgataa accagacttt
     9421 acccattgct gaatgcacgg gtaaagttag gctcaaataa ttaaacaaca cgttacagga
     9481 caacaggatg caaaatcggc tgaccatcaa agacatcgca cgcttaagcg gcgtggggaa
     9541 atctacagtt tcccgggtgc tgaataacga aagcggcgtg agccagcgca cccgcgagcg
     9601 tgttgaagca gtgatgaatc agcatggatt ttccccttcc cgctctgcgc gcgctatgcg
     9661 tgggcaaagc gataaagtgg tcgccatcat tgttacccgc ctggattcgt tgtcagaaaa
     9721 tctcgccgtt caaaccatgc tgccagcgtt ctatgaacaa ggttacgacc caatcatgat
     9781 ggaaagtcag ttttccccgc aattagttgc cgaacatttg ggggtgctga aacggcgtaa
     9841 tatcgacggc gtagtgctgt tcggttttac tggcataaca gaagaaatgt tagcccactg
     9901 gcagtcatcg ctggttctgc tggcgcgtga cgcaaaaggt tttgcttcgg tctgttatga
     9961 cgacgaaggg gcaatcaaga tcctgatgca acggctgtat gaccaggggc atcgtaatat
    10021 cagttatctc ggcgtgccgc atagtgacgt gacgaccggg aagcgacgtc acgaagccta
    10081 cctggcgttc tgcaaagcgc ataaactgca tcccgttgcc gctctgccag gccttgctat
    10141 gaagcaaggc tatgagaacg tagcaaaagt gattacgcct gaaactaccg ccttactgtg
    10201 cgcaaccgac acgctggcac ttggcgcaag taaatacctg caagagcaac gcatcgacac
    10261 cttgcaactg gcgagcgtcg gtaatacgcc attaatgaaa ttccttcatc cggagatcgt
    10321 caccgtagat cctggttacg ccgaagctgg acgccaggcg gcctgccaat tgatcgcaca
    10381 ggtaaccggg cgcagcgaac cgcaacaaat catcatcccc gccaccctgt cctgatcgtt
    10441 tcctgaacga taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt tttaaatttt
    10501 tccgcgcaat atattctgca gccaaccaaa aatgtcatct gccatggggc tttatgatga
    10561 gcaaaataaa ccaaacggat atcgatcggt tgattgaact ggtcggcggg cgcggcaata
    10621 ttgcgacggt gagccactgt attactcgcc tgcgctttgt cctcaaccaa ccggccaatg
    10681 ccagaccgaa agaaattgag caactcccca tggtgaaagg ctgtttcacc aatgccgggc
    10741 aatttcaggt ggtgattggc accaacgtgg gtgattacta tcaagcactg atagcgtcaa
    10801 ccggacaggc gcaggttgat aaagagcagg taaaaaaagc cgcccggcag aatatgaaat
    10861 ggcatgagca gttgatctct catttcgcgg agatcttctt cccgttgctg cccgcgttga
    10921 ttagcggtgg tttgatcctc ggttttcgca atgtgatcgg cgatttgccc atgagcaacg
    10981 gtcaaacgct ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt ctgtggttga
    11041 tcggtgaagc gatcttcttc tacctgccgg tcggtatttg ctggtcagcg gtgaaaaaaa
    11101 tgggcggcac gccgatcctt ggtatcgtgc ttggcgtgac actggtttct ccacagctga
    11161 tgaacgctta tctgctcggg cagcagctgc cggaagtgtg ggactttggc atgttcagca
    11221 tcgccaaagt aggctatcag gcgcaggtga tcccggcact gttagccgga ctggcgctgg
    11281 gcgttattga aactcgcctt aaacgcatcg tgccggatta cctctatctg gtggtggtac
    11341 ccgtctgttc gctgatcctc gcggtgttcc tcgcccatgc gctgattggt ccgtttggtc
    11401 gcatgattgg cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc agctttgctc
    11461 caattggcgc ggcattgttt ggcttcctgt acgccccgct ggtgatcacc ggggtacacc
    11521 agaccacgct tgctattgat ttgcagatga ttcaaagcat gggtggtacg ccagtgtggc
    11581 cgctgattgc gctgtcgaat atcgctcagg gctccgccgt gataggcatt atcatttcca
    11641 gccgcaagca caatgaacgc gagatctccg tgcctgcggc gatctccgcc tggcttgggg
    11701 tcactgagcc tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg ctgtgcgcga
    11761 tgattggttc tggtctggca ggattgctat gcggcctgaa cggcgttatg gcgaatggca
    11821 tcggcgtagg cggcctaccg ggaattctct cgattcaacc gagctactgg caggtgtttg
    11881 cgctggcaat ggctatcgcc atcatcatcc cgattgtact cacctcgttt atctatcagc
    11941 ggaaataccg cctgggcacg ctggacattg tttaattttc ttcggggcgc aattgcgccc
    12001 ctcgcatttg caggaataac gtaatgacta atcttcccca ctggtggcaa aacggcgtta
    12061 tctaccagat ttatccaaag agttttcagg acaccacggg tagcggcacc ggcgatttac
    12121 gtggcgttat acaacgcctg gactatctgc ataaactggg cgttgatgcc atctggctaa
    12181 ccccctttta tgtctctccc caggtcgata acggttacga cgtagcgaac tatacggcga
    12241 ttgatcccac ctacggcacg ctggacgatt ttgacgaact ggtgacgcag gcaaaatcgc
    12301 gcgggattcg tatcattctc gatatggtgt ttaaccatac ctctacccaa catgcctggt
    12361 ttcgcgaggc gctgaacaaa gaaagccctt accgccagtt ttatatctgg cgcgatggcg
    12421 aaccagaaac accaccgaac aactggcgtt caaaatttgg cggtagtgcg tggcgctggc
    12481 atgcggaaag cgaacagtac tatttgcatc tctttgcacc agaacaggcg gatctcaact
    12541 gggagaatcc agcggtacgc gcagagctga aaaaagtctg tgagttctgg gccgatcgtg
    12601 gggttgacgg gttgcgcctg gatgtggtga atctgatctc caaagacccg cgtttccctg
    12661 aagacctgga tggcgacggg cgtcgcttct acaccgatgg accacgagca cacgagtttt
    12721 tgcacgagat gaaccgcgat gtgtttacgc cacgcgggtt aatgaccgta ggtgaaatgt
    12781 cctccaccag ccttgagcat tgccagcgat acgcggcact gacaggcagt gaattatcga
    12841 tgacctttaa ttttcatcac ctgaaggtcg attatcccgg tggtgaaaaa tggacgctgg
    12901 ctaaacctga ctttgtggcg ttgaaaacat tgttccgcca ctggcaacaa ggaatgcaca
    12961 acgtggcatg gaatgccttg ttctggtgta accacgatca gccgcgcatt gtttctcgct
    13021 ttggtgatga aggtgaatac cgcgtgcctg cggcaaaaat gctggcgatg gtgctgcatg
    13081 gcatgcaggg aacgccgtat atctaccagg gcgaagagat tggcatgacc aacccgcatt
    13141 tcacgcgcat tactgactat cgcgacgtgg agagcctcaa tatgtttgcc gagctgcgca
    13201 acgatggtcg tgatgccgac gagttattgg caatcctcgc cagtaaatcc cgtgacaaca
    13261 gccgcacgcc catgcaatgg agcaacggcg ataatgccgg gtttacggct ggcgaaccgt
    13321 ggattggcct gggcgataac tatcaacaaa tcaacgtaga agccgcgctg gccgatgact
    13381 cctcggtgtt ttacacctac caaaagttaa tcgcactgcg taagcaagaa gccatcctga
    13441 catggggcaa ttaccaggat ctgctgccaa acagccctgt attgtggtgc tatcgccgtg
    13501 aatggaaggg gcaaaccttg ctggtcattg ccaaccttag ccgtgagatc caaccctggc
    13561 agccagggca aatgcgcggc aactggcagc ttgtgatgca taactacgaa gaagcctcac
    13621 cacaaccctg tgccatgact ttacggcctt ttgaggctgt ctggtggtta cagaagtaag
    13681 tatccgctaa gccccgggaa tgcctgtcat tccggggttt tgtctgtcag catgttgttt
    13741 ttgttgattt ttcaaccagc aaattcatta aaaaatttac ataacgctgt agcgcccgtc
    13801 atccgtacgc tctgcttttt actttgagct acatcaaaaa aagttcaaac atccttgatg
    13861 caaagcacta tatatagact ttaaaatgcg tcccaaccca atatgttgta ttaatcgact
    13921 ataattgcta ctacagctcc ccacgaaaaa ggtgcggcgt tgtggataag cggatggcga
    13981 ttgcggaaag caccggaaaa cgaaacgaaa aaaccggaaa acgcctttcc caatttctgt
    14041 ggataacctg ttcttaaaaa tatggagcga tcatgacacc gcatgtgatg aaacgagacg
    14101 gctgcaaagt gccgtttaaa tcagagcgca tcaaagaagc gattctgcgt gcagctaaag
    14161 cagcggaagt cgatgatgcc gattattgcg ccactgttgc cgcggttgtc agcgagcaga
    14221 tgcagggccg caaccaggtg gatatcaacg agatccagac cgcagttgaa aaccagctga
    14281 tgtcgggtcc atacaaacaa ctggctcgtg cttacatcga gtaccgtcac gatcgcgaca
    14341 ttgaacgtga aaaacgcggt cgcctgaacc aggagatccg tggtctggtc gagcagacca
    14401 acgcctcgtt actcaacgaa aacgccaaca aagacagcaa ggtgattcca acccagcgcg
    14461 acctgctggc cgggatcgtg gctaaacact atgcacgcca gcacctgctg ccgcgtgacg
    14521 tggtgcaggc acatgagcgt ggcgatattc actatcacga tctcgattac tcgccgttct
    14581 tcccgatgtt caactgcatg ttgatcgacc tgaaaggcat gctgacccag ggctttaaaa
    14641 tggggaacgc cgagattgaa ccgccgaagt cgatctctac ggcaaccgcg gtaactgcgc
    14701 agattattgc tcaggtcgcc agccatattt atggcggcac caccattaac cgtatcgatg
    14761 aagtgctggc accgtttgtc accgccagct acaacaaaca tcgcaagacc gcagaagagt
    14821 ggagcatccc ggacgcagaa ggctacgcta actctcgtac cattaaagag tgctacgatg
    14881 ccttccagtc gctggagtac gaagtaaaca ccctgcatac cgccaacggt caaacgccgt
    14941 ttgtaacttt tggttttggt ctgggcacca gctgggaatc gcgcctgatt caggaatcta
    15001 tcctgcgtaa ccgtatcgca ggtctcggta aaaaccgtaa aactgcggtg ttcccgaaac
    15061 tggtgtttgc gattcgcgat ggtctgaacc ataaaaaagg cgatccgaac tacgacatca
    15121 aacagctggc gctggagtgc gcaagcaagc gcatgtatcc ggatatcctg aactacgatc
    15181 aggtagtgaa agtcaccggt tcgtttaaaa ccccgatggg ctgccgcagc ttcctcggcg
    15241 tgtgggaaaa tgaaaacggc gagcagatcc acgatggtcg taacaacctc ggcgtgatca
    15301 gcctgaacct gccgcgtatt gctctggaag caaaaggcga tgaagccacc ttctggaagc
    15361 tgctggatga acgtctggtg ctggcacgta aggcgctgat gacccgtatc gctcgtctcg
    15421 aaggcgtgaa agcgcgtgtg gccccgatcc tctatatgga aggcgcttgt ggcgtgcgtc
    15481 tgaatgctga cgatgatgtt tctgaaatct tcaaaaacgg tcgtgcatct atttcgctgg
    15541 gttacatcgg catccacgaa accattaacg cgctgttcgg cggcgaacat gtttacgaca
    15601 acgagcagct ccgcgcgaaa ggtatcgcga ttgttgaacg tctgcgtcag gcagtggatc
    15661 agtggaaaga agaaacgggt tatggtttca gtctctacag cacgccgagt gaaaacctgt
    15721 gcgaccgctt ctgccgtctc gatactgctg agtttggcgt ggtgccgggc gtaacggaca
    15781 aaggttacta caccaacagt ttccacctcg atgtggagaa gaaggtgaac ccgtacgaca
    15841 agatcgactt tgaagcgcct tacccgccgc tggcgaacgg tggtttcatt tgctacggcg
    15901 agtatccaaa cattcagcac aacctgaagg cgctggaaga tgtctgggat tacagctatc
    15961 agcatgtacc gtattacggc accaatacac cgattgatga gtgctacgag tgtggcttta
    16021 ccggtgagtt cgagtgcacc agcaaaggct tcacttgccc gaaatgtggt aaccatgacg
    16081 cctcccgtgt gtcggtaact cgccgcgtgt gcggatattt aggtagcccg gatgcacgtc
    16141 catttaacgc cggtaagcag gaagaagtta agcgccgcgt taaacatttg ggaaatgggc
    16201 agataggtta atcttcactc tacgttaagc aaatgccgga tgcggcgtaa acgccttatc
    16261 cggcctacat ttggcaactg ctccaggcct gataagacgc gacagcgtcg catcaggcat
    16321 tgattaccgg atgcagccta cacgcggact gataagccag gcgaaataat gaattatcat
    16381 cagtactatc ctgtcgacat cgtcaacggc cccggcactc gctgcaccct gtttgtctcc
    16441 gggtgtgttc atgaatgccc cggttgctat aacaaaagca catggcgggt aaattctggt
    16501 cagccattta ccaaagcaat ggaagaccag atcattaacg atctgaatga cactcgtatc
    16561 aaacgccagg ggatttccct ctccggcggc gatccgctgc atccgcaaaa cgtgccggat
    16621 attctgaaac tggtaaaacg catccgcgcc gagtgtccgg ataaagacat ctgggtgtgg
    16681 acaggctata aactcgacga tctcaacgct gcgcaaatgc aggttgttga tttgattaac
    16741 gtactggtcg acggcaaatt tgtgcaggat ttaaaagacc cttccctgat ctggcgcggc
    16801 agcagcaatc aggtggtgca tcatttgcga tgataagttc cctgttacct gagtgcaggc
    16861 aacagggaaa tgaggaatta acgatacttc tggtgatagg cgttgcgggt cgttttcagt
    16921 tgctctgcgg cagcctgcgc ttcttttact ttaccttcat ttgccagctt cagcgcgtcg
    16981 tcaatctgac caaccagaat atcgaaaccg tgacggaagt ctttcatttc cgggctgtcc
    17041 ggtgatttat cttcgagctt cggcggcgtt gctttttgcg catccagcgc tgcggcgcgc
    17101 atcttcgtta acgcgtcttt gacttgcgcc gcgttatcgg ctttttcgac cacttttaaa
    17161 gtgtcgttga gggtttccat attgtcttca agatcagcgg caaacgacgc cgaactgaat
    17221 accaacgagg agactgcaag aatagctaac aggcttttac gcattgctca cttccttttt
    17281 tatttacact tcatccttca cactgcctca tcgacggcta cgctcattcg cccgggtcac
    17341 gtactgcgag tacgctcccc ggccctcatt cactttccgt ctcgatgcag tgcgaatgat
    17401 tttgtgtaat tttttagttt atagcgcggc aggtcgcgcc agttttttac tgcccggcga
    17461 ttttcatctc cggcaacagc acagaaccac actgtatatt actgcgtgtt tcaatatcgt
    17521 taccgacggt gacaatattg cgccacatat cttttaaatt acctgcgatg gtgatttcgc
    17581 taaccggata ctgaatttcg ccattctcta cccagaagcc cgctgcacca cgggaataat
    17641 caccggtaat ggcactcacg ccctggccca tcagctccgt caccaccagc ccggtaccca
    17701 tctctttgag catttgttcg aagcttagcc cttgtccggc aatccgccag ttgtgaatac
    17761 cgcccgcatg tccggtgctt ttcagcccca gtttccgcgc cgagtagctg gtcagcagcc
    17821 actgagtcag gatgccatct ttaataatat cgcgacgctc ggtgcgcaca ccttcgctgt
    17881 caaatggcgt cgatgccagc cctttcagca gatgcggatg ctcttcaatg gtcagccagt
    17941 ccggcagaat ttgtttaccc agcgaatcca gcaggaaggt ggatttacgg taaaccgatc
    18001 cacccgctat cgcccccacc agatggccaa aaagcccggt tgccacttca ttggcaaaaa
    18061 tgaccggcgc tttcatggtg gagagtttac gcggtgacag gcgcgataaa gtacggcgag
    18121 cacagtcggc cccaacccac tctggcgttt gcagatcgct catcgcacga ccaatggtgt
    18181 aggcgtaatc acgctccata tcgccatttt cttcggcaat tacacagctg gagagcgaat
    18241 gacgcgttga gcagtaaccc tgcaacatgc cgtggctgtt gccaaaaact ttgacaccgt
    18301 agtggctgtt aaagctaccg ccttcggtat tggtgatgcg tttgtccgcc tgcaatgctg
    18361 cctgttctgc gcgggccgcc agttcaatgg cttcatccgg ggaaacttcc gcagggtgga
    18421 acaaatcgag atccggtgcg tcaaaggcca gcagctcttt gtctgccacg ccggcacagg
    18481 gatctggcga ggtataacgg gcaatatcca gcgccgcctg tacggtgcgg gcaatggcct
    18541 gcgggcttaa atcggtggat gatgcgctac ctttgcggtt ctggtgataa acagtgatcc
    18601 ccagcgcgcc atcgctattg aattcaacat tctccacttc accataacgc gtgcttacgc
    18661 taatgccggt ggtcttgctg acggcaactt ccgcaccgtc cgatttgcct gaggccaact
    18721 ccagcgcagt ggaaactgct tcttccagaa tcttgcgctg cgcttcaact tgagagatta
    18781 ctttcattgc aagtgccata atgtagagag tttctctgaa gtctaacaga gaaccgtttt
    18841 tcagtgcgca tcttaactgg taacattagc ctcttttttt aaggagcctg agatgactaa
    18901 gcagcccgaa gactggctcg acgacgttcc cggtgatgac atcgaagacg aagacgatga
    18961 aattatctgg gtcagtaaaa gtgaaattaa acgtgatgcc gaggagctaa aacgccttgg
    19021 cgcggaaatt gttgatctgg ggaaaaacgc gctggataag atcccgttag atgcggatct
    19081 gcgcgcggct attgagctcg cccagcgtat taagatggaa ggtcgccgcc gccagttgca
    19141 gctcatcggt aagatgctgc gccagcgcga cgtagagcct attcgtcagg cgctggataa
    19201 gctgaaaaac cgtcacaacc agcaggtggt gctgttccat aaactggaaa acctgcgcga
    19261 tcgtctgatc gatcaaggtg atgacgccat cgctgaagta ttgaatctgt ggcccgatgc
    19321 cgatcgtcag cagctgcgta ctctgatccg caacgcgaag aaagagaaag aagggaataa
    19381 gccgccgaaa tccgcacgcc agattttcca gtatctgcgc gaactggcgg agaatgaagg
    19441 gtaattttcg ttgttgatgt gagtatggtt tgccggatgc ggtgcacctg taggcctgat
    19501 aagacgcatc aagcgtcgca tcaggcacta tgctcaaatg ccggatgcgg cgtgaacgcc
    19561 ttatccggcc tacgcacact atatcaggct aaggccgaat tactgcgcgg cttccgcctt
    19621 cttcgccaga ccatccagca gtttctgatg gatcccacca aaaccgccgt tgctcatcac
    19681 cagaatatgg tcgccaggct gagcggtttt caccaccata tctgccagcg tatccacatc
    19741 gccactccag tgtgcaggct gaacgcaggc ttctgccact tctgccacct gccacggaat
    19801 atgcgccggt tgcagcagga agacttcatc ggcacgacct aatgaaggtg ccagatcgtc
    19861 tttgcagatc cccattttca tggtattcga gcgcggttcc agcacggcaa taatgcgcgc
    19921 cgtaccgcca actttgccac gcagcgccgc aagcgttgcc agaatcgccg ttgggtgatg
    19981 ggcaaaatcg tcatataccg tgacgccatt cgcttcacca cgcaactcca gacggcggcg
    20041 agcgttaata aacgaaccca gcgcgttagc ggcatctgcc ggcgctacac caacatggcg
    20101 agccgccgca atcgccatca ggccattgtg catattatgt tcgcctacca gcgaccattt
    20161 cacttcgccc actttttcgc catccagcaa gacttcccat tcggaagcat cggtggtcag
    20221 ctttttcgcc tgccagtgac cctgctcacc caccagctcc tgctcgctcc agcagcccat
    20281 cgccatggtc tgtttcaggt tgatgtcgtt ttccggccag ataatacggc cctgccccgg
    20341 aacgatacgc accagatggt ggaactgttt ctggatcgct ttcaggtcgt caaagatatc
    20401 ggcgtgatcg aactcaaggt tgttgaggat cagcgtacgc gggcagtaat ggacaaattt
    20461 agagcgtttg tcgaagaagg cgcagtcata ctcatccgct tcgataacaa agaagtcgct
    20521 ttcgcccaga cgcgccgaaa cctcaaagtt ccccggcaca ccgccgatca caaatcccgg
    20581 tttgtaaccg cactgttcca gaatccaggt cgccattccc gcggtggtag ttttgccgtg
    20641 tgtaccggca acggccagca cccagcggtc gcgcagcaca aaatcgtgca gccactgcgg
    20701 acctgacata taagggatgt ttttttccag taccgcttcc acacacggat ttccacgggt
    20761 catggcgttg ccaataatca ccagatccgg ctgcggatcg agctggctgg catcgtaacc
    20821 ctgaatcagt tcaatgcctt gcttctcaag taaggtgctc atcggcggat acacattggc
    20881 gtccgaaccc gttacttcat ggcctaactg gcgcgccagc atcgccagac cgcccataaa
    20941 cgtgccacaa attcctaaaa tatgaatgcg catacgtcac tatccttttt taatctgccg
    21001 ctcattttac gcatatgtcg ggcaagtgag aaacgcattt caggataatc cgcaatttgc
    21061 tggcgcgatt cacctgagcg caacattccg attatttgtt aagattgtta cggtcgcttt
    21121 actccataaa cattgcaggg aaagttttat gaaaacgtta ggtgaattta ttgtcgaaaa
    21181 gcagcacgag ttttctcatg ctaccggtga gctcactgct ttgctgtcgg caataaaact
    21241 gggcgccaag attatccatc gcgatatcaa caaagcagga ctggttgata tcctgggtgc
    21301 cagcggtgct gagaacgtgc agggcgaggt tcagcaaaaa ctcgacttgt tcgctaatga
    21361 aaaactgaaa gccgcactga aagcacgcga tatcgttgcg ggcattgcct ctgaagaaga
    21421 agatgagatt gtcgtctttg aaggctgtga acatgcaaaa tacgtggtgc tgatggaccc
    21481 actggatggc tcgtccaaca tcgatgttaa cgtctctgtc ggtaccattt tctccatcta
    21541 ccgccgcgtt acgcccgtcg gcacgccggt aacggaagaa gatttcctcc agcctggtaa
    21601 caaacaggtt gcggcaggtt acgtggtata cggctcctct accatgctgg tttacaccac
    21661 cggatgcggt gttcacgcct ttacttacga tccttcgctc ggcgttttct gcctgtgcca
    21721 ggaacggatg cgcttcccgg agaaaggcaa aacctactcc atcaacgaag gaaactacat
    21781 taagtttccg aacggggtga agaagtacat taaattctgc caggaagaag ataaatccac
    21841 caaccgcccg tatacctcac gttatatcgg ttcactggtc gcggatttcc accgtaacct
    21901 gctgaaaggc ggtatttatc tctacccaag caccgccagc cacccggacg gcaaactgcg
    21961 tttgctttat gagtgcaacc cgatggcatt cctggcggaa caagcgggcg gtaaagcgag
    22021 cgatggcaaa gagcgtattc tggatatcat cccggaaact ctgcaccagc gccgttcgtt
    22081 ctttgtcggc aacgaccata tggttgaaga tgtcgaacgc tttatccgtg agttcccgga
    22141 cgcgtaatct cctgcgcctc tccacacgga gaggcgtttt tatcgctgcg caatgttgac
    22201 tcttgtcact ggcgaactct gacgattctc ccacagcacc gttaatccac gctgtaatgc
    22261 tataaaaata aacaacaaaa taccgatggc gattttcgtc caccatgagc tcagcgtgcc
    22321 atcaaagttt atgtaagtct gaatcagtcc ctgaatcgcc acgccaaaaa gcgtccctaa
    22381 taccgttcca acgccaccgc tcagaagcgt accgccaatt accactgagg cgatagcatc
    22441 cagttccaca cctacgcccg ccagcgcata tccggcctgg gtataaatcg agaagacaat
    22501 ccccgccagc gttgccagtc cggtggagag catataaatg cgaatagtgg tgctgcgagt
    22561 ggaaatcccc atcaggttcg ccgacgttgc gttgccgcca atggcgtata cctgattacc
    22621 aaaacgggta cgatgcgcga ggaaaatacc gataaccacc accgccaaca tcagcagtcc
    22681 catcgcactt aagcgaccgc cgccagggat tttccacgca aggcttgaga gcgtgtcata
    22741 aatgggatgg tttatcggaa tcgactcttc cgaaacgaga tagctgacac cgcgcaaaaa
    22801 gaacatcccc gccagggtaa tgataaatgc cgggatcttc agggcgtcga tcaaaagccc
    22861 cataaatgcg ccgaaggcac agcccatcac cagcactagc ggaaacgcca gtagcggcga
    22921 gaggccgaaa tcgccaatca cttttgccag aaacacgcca gtaaaggcga tcaccgaacc
    22981 gacggagaga tcgatcccac cggagaggat cacaaaggtc atgccaacgg caatgatccc
    23041 aagaaaggcg ttatcggtca ggatattgca gatcactctt gtggaagcaa aaccgggaaa
    23101 ctgggtcagg cagtaaagat aacccaacac aaagacgccg atggtgatca tcagtggcaa
    23161 attacgtttt atcacggcta cgtactcctt taatcagact gataaagcgt tgcgactgga
    23221 caatcagcac gcaaagaacc accaccgctt ttacaacctg gttcatctct ggcggaaagc
    23281 ccgaaagcaa aattccggtg ttcatcccct gaataatcag cgcccccacc accgaaagca
    23341 gtaggttaaa acgtccgccc atcagcgatc cgccgccaat aaccaccgcg agaatggcgt
    23401 ccagctccag ccataacccg gcgttattgg catcggcacc gcgaatatcc gccgccacga
    23461 taatgcccgc aatcgccgca cacagcccgc tcaacacgta ggtaagcatg acgatgatcc
    23521 gcgtgtttac cccggcattt tttgccgccc gaatgttgat accaacggct tcgataaaca
    23581 tccccagcgc cgttttgcgg gtcaacagcc agaacaggat aagcgtcagc accgcgataa
    23641 tgaccggcgt tggcaggaac aacagcgatc cactgccaaa ccatgagaga tccggcgagt
    23701 taaacgtgac gatctgtccg gaggtgatca gttgcgccac gccgcgcccg gcgaccatca
    23761 ggatcagggt ggcaacaaac ggctgaattt tgaggatcgc taccagtatg ccgttccaca
    23821 atcccgccag gatgccagtg cccagggcgc ttaacaaaac aatcggcagg ctgaatcccg
    23881 cgaccgtcat cgcagccgtt gtggctccgg cgatagccat caccgccccc acggagagat
    23941 caatcccacc tgttgcgatc accagcgtca ttccgatagc caacagcgca acgggggccg
    24001 cacggttaag aatgtctatg gggctaccga ataaacgccc atcctggagc accacctgcc
    24061 agaaatgcgg ggccaccagg ctatcgacca gcagcaccag caacagtgcc gccagctgcg
    24121 gcattcccgt tggccagcga aagcgccttt tcggcggcgt agtgtccggg agagattgag
    24181 gcatcacact gttctcctta cgccgcaatg gcgttcatga tcgccggaac ggaaagctct
    24241 gccagcggga tctccgccac ctgtttgcga tcgcgcatga tgatcacccg gtcggcatag
    24301 cccaccagtt cttccagttc ggaggagatc accagcagcg ccagaccatc ggcgcatagc
    24361 gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat caatgccgcg agttggctca
    24421 tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg aaagcaacac tttttgctga
    24481 ttgccgccag agagaaattc aatcggttgt tcagttgaag gtgtgcgaat gccaagctgg
    24541 cggataaagc gttcggcaat ctcttgctgt tctttgcggg aaatgggacg tagccagccg
    24601 cgctgggcct ggagagcgag gatgatattt tcccgcaccg aggcggcagc gatgatgcca
    24661 tcggttttcc tgtcttccgg gcagaagcca atgcccagta ccgaagcctg atgtggcgat
    24721 cgcaggtttt gcggtttgcc tttgatcaac gccgtgccgc tgtcagcagg tttgataccg
    24781 aagatcactt cggcggtttc ggtacgtcct gatcccagca atccagccag accgacgatc
    24841 tcgccggggc gtacttcgag atcaaacggt gcgatcgttc cttttttgcc gtaatttttg
    24901 aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg cacgctgtag cgcgtgggta
    24961 tccagctcgc gccccagcat catttttacc agttcgatct gcggtagctc gcacgtttcc
    25021 cgacagccta cgaaactgcc gttgcgtaag acggtgatcc gatcgctgac ctgatagacc
    25081 tgatcgagaa agtgagtgac aaaaatcagg ctgacgccgc gatcgcgcaa ctgacgcatc
    25141 aggtcaaaca gtaactccac ttcctgggtg tcgagactgg cggtgggttc atcgaggatc
    25201 agcactttgg cagagagatc gatagcccgg caaatagcga cgatttgctg catcgcgact
    25261 gaaaagcggt tgagcggttc gcgcacgtcg agggagaaac cgtaagatgc catcagttcg
    25321 gtggcgcgct tttccatctc tttgcggcgt agaaggccga agcgtttggg ttcgcggcct
    25381 ataaatagat tatcagcgac cgacatattg ggtagcaggt ttacttcctg atagacggtg
    25441 ccgataccga gttgttgtgc atgggcggta tttttcggtg agatagcctg gccttccagc
    25501 cagatggtgc cgcgatcggc gtggtataca ccagttaatg ctttgattag cgttgatttt
    25561 cctgccccgt tttcaccgag cagcgccatg atttcgccgc ggcgcaggct gaaatcaacg
    25621 ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc cttcggtgcg gaggatctcc
    25681 tggtgttggt tagtcgtcat ggtttccccc ggtgtaaagc gtgctcccag taggcatgat
    25741 aagacgcggt aagcgtcgca tcaggcttca gcgcaccacc gccggatgcg gcgcgagcgc
    25801 cttatccggc ctacgaatcg gcatcaggca tagcaaccct caatacccca tatttttctt
    25861 cttctctaac tcttcttttg cggtatcagg cagataaagg gtggatttgg tcagcgtcag
    25921 cttttcaggc atggtgccgt cttttttgta tttctccagc gcgtcgaagg cgggacctgc
    25981 catattcggc gtcagttcaa cactggcgtt cgcttcgcca tcaatcatcg ctttgtagat
    26041 atccggtacg ccgtcgatag aacctgtcag aatatctttg cctggtttca ggcccgcttc
    26101 tttaattgcc tgaattgcac caatcaccat gtcgtcgtta tgggcgtaaa ccatgcagat
    26161 gtttttgccg ttgttttccg ctttgataaa gctctccatg acttctttgc ctttactgcg
    26221 ggtgaagtca cctgactgcg agcggataat tttgatattt ggcgcattct taatggcttc
    26281 ggcaaagcct ttcttacggt caatggcgac gctggcccca acagtgccct gcagctccac
    26341 cacgttgcat ggtttgccat tcacttcttt taccagccag tcaccaatca acttgccttc
    26401 gagaatgttg tcggcagtga cggtggtcat atagagagat ttgtctttca catcaataga
    26461 acgatcgagc aagaagaccg ggatttcggc atctttcgcc tcttttaata ccggttccca
    26521 accagtcgca accaccggag cgataaagat cgcatccacc ccttgcgcga cgaaggaacg
    26581 taccgcttta atctggtttt cctgcttttg ctgaccatcg gcaattttca gcgtaattcc
    26641 gcgtttttcg gcctcacttt tcgccacatt ggtttctgcg gcgcgccagc cagattccga
    26701 tccgacctgc gaaaatccta cggttaatgg agcggccaac gccatagacg acatggctgc
    26761 cgagactgca gagactacaa gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt
    26821 attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc
    26881 gttacatcac acttcaaaga agtaaataaa acgttaatca caagtttgta atcgctttca
    26941 tctcactatg aaaaatgcgg ctacggttat ggattttcct gctctgtata ccgtcttaaa
    27001 attggcgaaa aaggaaaatg aagacgaaaa caagcgaaga cattcggcgc gagttggcta
    27061 taatactcgg cacttgtttg ccacatattt ttaaaggaaa cagacatgag cttactcaac
    27121 gtccctgcgg gtaaagatct gccggaagac atctacgttg ttattgagat cccggctaac
    27181 gcagatccga tcaaatacga aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc
    27241 atgtccaccg cgatgttcta tccgtgcaac tacggttaca tcaaccacac cctgtctctg
    27301 gacggtgacc cggttgacgt actggtcccg actccgtacc cgctgcagcc gggttctgtg
    27361 atccgttgcc gtccggttgg cgttctgaaa atgaccgacg aagccggtga agatgcgaaa
    27421 ctgattgctg ttccgcacac caagctgagc aaagaatacg atcacattaa agacgttaac
    27481 gatctgccag agctgctgaa agcgcagatc gctcacttct tcgagcacta caaagacctc
    27541 gaaaaaggca agtgggtgaa agttgaaggt tgggaaaacg cagaagccgc taaagctgaa
    27601 atcgttgctt ccttcgagcg cgcaaagaat aaataagttc ttctagcgca ataaccctga
    27661 acgccgggct tcggttagta agggtttttt tatgcccgcg ataaataaac tctctattcc
    27721 accatcatta ttctcagcgg ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac
    27781 cattttgctg ttcatagcca cttgctggaa gttagccgac ctcactcata ctcaccgttc
    27841 aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga
    27901 ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa
    27961 atcgcgagat acatcgcata aaacaacgcc acccgaaggt ggcgtgcttt tattattact
    28021 tatccctgtc taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat
    28081 aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc
    28141 ccctggtgcg caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac
    28201 cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt
    28261 agttatcgat actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt
    28321 gtttgtggcg taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag
    28381 caaatccagt gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg
    28441 ataacgtaac gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag
    28501 acctggcaat acatagccgc tgaccactac ctgggaggag tcgcctactc cctgggtgtc
    28561 gagcgctaaa ttgcttacgc caaacgtctc gccgatttta cccacaatct ggccactttg
    28621 cgcaaccccc agaccaatca gcatcgaggt cattgccgca ctgtcgctct gatcgctctc
    28681 cagcccttgt ccacgtagca aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga
    28741 gaagatctcc gctttcggtt cgtccgccag accagtgacg cgaactccgg cgattacgtc
    28801 gtcttctgta gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg
    28861 accagagaac agtaactcac ctttacgcac aatcagatcc tgaccatagg catggaagcg
    28921 cccttcaggg atgttgatct gcccgttcag gcccagccct tgtttgtcct gaacgacgtt
    28981 aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac
    29041 gtggacaatc aggttactgt taatcggaat cgacgccgtt ttcggctctt ccggttgcag
    29101 gttatcgtta agcatcacca catcgctgga gacgcctact gcgctttccg gcagatcgtg
    29161 caccacgatg cgcgcccacg ggacatccac gcgaccatcg agggtaaaca ggtttggtgt
    29221 agcctcgaag acaacatctg gcgatacatc cattcgtacc atcggcggca cggtgatccg
    29281 cactttactg cctttcgccg ttacccgcgc ccgccagttt tcaatttggc tccagtcggc
    29341 atcaccgttc aggtagattt caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg
    29401 cataccatta aagttgaccg caagctggct cggttgcata tcaaacggca taaagttgcc
    29461 gtcgatatcc acacccgtaa cctgaagctg accaaacaac tgcgggcttt gcacatcacc
    29521 acccagacgc aagttggcac tcaccatccc cgctgctttt tccccacggg taaagatggg
    29581 gtttatcatc gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc
    29641 ttgcggatcg gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt
    29701 ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc
    29761 cacgggcagc gccgcatcgt tgacggtttg cgttacctgc acgttacgcc ccgaaagggt
    29821 gatactgccc tgcggcagcc cctctttggt ggtgtcccag gcaacgtccg ctttacccgt
    29881 gaagataccg ctggcctgag tggtttctgg cataaatggt ttcagcatgg ccaggtcgaa
    29941 gcggttgaga ttcaccaccg cacgcccttc ggcacccgca tcgatagttt gcggcacgca
    30001 cagttccgca ttcgggttaa gccagcagtg tggcccgatg ctgatttttt gctccttatt
    30061 gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg
    30121 ggtattgcta agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt
    30181 aagctgcccg gagacaggct cgccctgaat ccgcaactgt aactcgtgct gtttttcgct
    30241 gcctttggca ttcagggtga cgaggttgat atttacatcc ggctgcgaaa tttgctcaac
    30301 gcgtacgtcg agtttcccgg cgatctgatc ggtggattta atgtcgcctt ccacgcgaac
    30361 ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg
    30421 tggcgcttcc accgtgccgc gtactttcac cagccctttc gctgtaccgc caagccccgg
    30481 cagcgcgtta tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc
    30541 cagctcgcct ttcacttcgg cactgtttgg ccccagttcc agatgaagcc ctgggatcat
    30601 ccactgcata taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac
    30661 gttaccggtc agcttcagtt ctggcacctc catctgccag gtgccgccgt acaggctacc
    30721 gcgggtttta atcaagccat tgagtttcga cggccactct gggatctctt tggcggtgtt
    30781 aatgccgtta agcgttagct caccgcgcca actaatggcc tgctgccagt cgagcaacgc
    30841 cttgagttca gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg
    30901 ttcattacct ttggcatcaa gggtaatggt ggctggcggg atctccagtc ccttcactgc
    30961 cgtacgcata gagagcgtgt aatcggtcat tttgccggta agtttcagtt tcagatcatc
    31021 cgcctgatac tgcttctcac cagtgaacgg ccagtaaatc tgtttgctgt tcacttccac
    31081 gttgagcggc aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg
    31141 accggaaaga ttaacgccaa tctccagctg ttcgcgcagc gcgccgccca ctttcagctt
    31201 caccttttca cctttcaacg gctccacgtt cagtgtacta ttgagagtga tgtccaccgg
    31261 ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg atcccttgac tggaatcgat
    31321 atccagggcg tccagtttag tattgccgtc aatgctgctc actttcagca gcatggtgct
    31381 cacggtgatg tccgtgtcgc ccgtcacgcg caactgctcg cccttaaact cttcaatgtt
    31441 caggttaagc ggcaaatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc
    31501 tttcagcgtt tcgccgagcg gcttttcctc cggctgcgga ttttcaattt tcggttcgac
    31561 cacttcttcc tgcgccactt ccgccacttt cggcagagca atcagcaggc ctttcagcga
    31621 cgtcggtttc agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac
    31681 cgataccgtg gtgtcatcaa tcttgatgtt gacgttgtcc agtgccaccc gtgttagggt
    31741 gatgggatac ggcgtggaga gatccagcgg accgctatct tcctcttctt caacctgttc
    31801 agaagggggc atttttttgc tgtcgatatt gacctggatg tctttcagcg ccagatcatt
    31861 aatacaaaca ctgctgttcc acaggcactc aagcccgacc gccagatgca gattgcctgc
    31921 ttttaccgcc acgcctggct gctcataacg aacgtcagac aaggtgagat cgcgccagcc
    31981 gccggtgacc ttgccaacat ccagtcctgg cacccagcga tccgccgctt taaataccag
    32041 atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac
    32101 gataaccacg ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg
    32161 taaaactgta acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt
    32221 ggcccgaccg gcgattccca gcgtacgccg accccggtac cggttttaaa gtcgctgcgg
    32281 cgaatatcgc ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc
    32341 acgttgtact ggtactccag cgatccggtt atcaacttcg aggccccttt caggtcaccg
    32401 ttggcgtatt tcggagcgat agatttgtat ttgtagccac gaatactgcg gtcgcccccg
    32461 gcgaaaaaac gcagatccgg cggtactttg tcgaaatcac cggtttcaat ccagcccagc
    32521 gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc ggatccagac gttttgtgcc
    32581 tggaaaacgg agaaatcgac atctgagccc caggcagtgt tggagtagtc gatagagtag
    32641 cgttgcgagt cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc
    32701 accccaggat aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa gtggtcgaga
    32761 ctccagcgca ggttaatggc acgctgccag ccgctggaga gatcccagta gcgagaagcc
    32821 accagcgtag tggagtcaga ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc
    32881 accaaataat attgttccag tggattcttc agcagcggca ttttatagct gaagtcgagg
    32941 atctgttccg gcgcggaaat actggtactg gtggtcagac tgtgaccgta tgagttcatc
    33001 cacggttttt tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg
    33061 gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt
    33121 tcgcgcgctt tatcaaattg tggagccacc accaccgagt taaaccagcc ggtagcagaa
    33181 agtcggcggt tcagttccgc cagatctttc gattcgtact catcgccctc tttaaacggc
    33241 accagatttt gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag
    33301 cggtaacgtt cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca
    33361 atgcccagct gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatgctg
    33421 gttaaggact ttttgaaatt ttcataatcg ccctggttca gcaccgtgcc aatagccggg
    33481 cgagtatcga gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg caataccaca
    33541 tcggtgccgc caattaacac cggcacgcct ggcgtgactt tggcgatcaa tacctgccgc
    33601 cctttctttg gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct
    33661 ttcagacctt cgcggatggc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact
    33721 tcatcacttt caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac
    33781 ccctcgacct gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt
    33841 acacagcata actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctgccc
    33901 ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa
    33961 accccaaata tttcttatgt tttactttag acctattcac ggtggttatt gtgtgcaaat
    34021 acgcctcttg ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt
    34081 ttatttgata aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg
    34141 atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga
    34201 atggagattg ccatttttgc gatgggttgt ttctggggtg tggagcgtct gttctggcag
    34261 ttacccggcg tttacagcac cgccgcaggc tataccggag gctatacgcc aaatccgact
    34321 tatcgggaag tgtgctccgg tgatacgggt catgccgaag cggtacgcat tgtttacgat
    34381 ccttccgtca tcagctatga gcagttgcta caggtatttt gggagaatca cgatcccgcc
    34441 cagggaatgc gtcagggcaa tgaccacggc acgcagtatc gttcagcgat ttatccgctg
    34501 accccagaac aggatgccgc agctcgcgcc agtctggaac gttttcaggc ggcgatgctt
    34561 gccgccgatg atgatcgtca catcaccacg gaaatcgcta acgccacacc gttttattat
    34621 gccgaagatg accaccagca atatctgcat aaaaacccgt atggttactg tggaattggc
    34681 ggaattggcg tctgtctgcc accggaagca tagcgttacg ggtacaaatg tagattgttg
    34741 ataaagtgcg ctttatttat gccagatgcg gcgtaaacgc cttaccaggc ctacaaaatc
    34801 gtccaaattc aatatattgc aaggactgcg taggcctgat tggcatagcg catcaggcag
    34861 tattgcattt atcagcagtc tgaattttaa ccctctggcg actttacagt accttacgct
    34921 atactagcca ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat gtgtatttca
    34981 cacaaattaa tcaacttccc cttccgagga tctggcctga aaggtcggat aagatatgtt
    35041 aaacagtatt ttagtcatac tctgcttgat cgctgtaagt gcgttcttct cgatgtccga
    35101 gatctcactt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat
    35161 aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta ccgtggtcca
    35221 aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc
    35281 agcttttcac agcctgttct cccgctatat gtcggcagag ctctctgagc aactgagctt
    35341 tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa
    35401 acgcatcggt atgattgcgc cagaagctgt cgctttgcgt atcatcaacc cgatgcgctt
    35461 ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac ggactggcga acataatctt
    35521 ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc
    35581 ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg agctgattga
    35641 aaacgtcttt gagctggaat cccgtaccgt tccgtcgtca atgacaccgc gtgaaaacgt
    35701 gatttggttt gatctccacg aagatgagca aagcctgaag aataaggtgg cggaacatcc
    35761 gcactctaag ttcctcgtct gtaatgaaga tattgaccac atcatcggct atgtcgattc
    35821 taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca
    35881 aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaggcgt tggaaagttt
    35941 taaaaccgca ggtgaagact tcgcggtgat catgaacgag tacgcgctgg tggtggggat
    36001 catcaccctc aatgacgtga tgaccacgct gatgggcgat ctagtcggtc aggggctgga
    36061 agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca ccccaattga
    36121 cgacgtcatg cgcgtgctgg atattgacga gttcccgcag tcgggcaact acgaaaccat
    36181 cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt
    36241 cgccggctac aaatttgaag tggtggatat cgataactac cgcatcgacc agctgctggt
    36301 gacccggatc gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa
    36361 agaagaaagc gtcgcgtaac ccccagaaac atcaacggct cctgaatcag gagccgtctt
    36421 attactgcat agcactttgg ttaagccatc tctgtttgca gacgcataac ctgacggtta
    36481 acttcggaca tcactgacaa atgcagctta tccttcactt ttgggataag aatcttaccc
    36541 ttatcaaatt caaaagcgcc aacgtccttg atgtataacc gtccacggaa caggatcttt
    36601 acgtacttcg ccacctgaag tgggttgtac cgttggaaaa ttttcattgt tatctcctgc
    36661 tgagtattac gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg caaattttaa
    36721 tgcttagtga ctatagacta tccgggcaat gtttccaccg cgtataactt tttttaccta
    36781 aaggttacaa ttattcagaa ttatcttttt accgaagcgc ggtcttcagt ataagcattc
    36841 atttttcata tgatttgtgc gcttgaccgc aaactggcat cacacttgcg ggaaattcga
    36901 taaatagcac atatgattaa aactcagacc caagtggtcg gatcacctgc atatcataag
    36961 aaggaaacac catgacccta cgcaagattc tggcactcac ctgcctgctg ttgccgatga
    37021 tggcttccgc acatcagttc gaaaccggtc agcgagtgcc gccgattggc attaccgatc
    37081 gaggcgagtt ggtgcttgat aaagatcagt ttagctacaa aacctggaac agcgcacagt
    37141 tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg cacctctgca aaagagaaaa
    37201 acgcgacgct gattgaagcg attaaatcag cgaagttacc gcacgatcgc taccagacca
    37261 ccaccattgt taacaccgac gacgcgattc cgggttcagg gatgtttgtg cgcagcagtc
    37321 tggagagtaa taaaaagctt tatccctggt cgcagtttat tgtcgatagc aatggcgtcg
    37381 cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt agtggtgctg gataaggatg
    37441 gtcgcgtgca atgggccaaa gacggggcgc tcacacagga agaggtgcag caagtgatgg
    37501 acctgctgca taaattaatt aataaataga cactctgaat cccggattca ggaacgactc
    37561 gcgcggagtg taatccagcg gtttaccctg ccagtcatga acgtgcgctc cggcagctgc
    37621 agccacagca tgaccagcgg cggtatccca aatattcgtt ggtccgaagc gcgggtacag
    37681 ctgcgcctgc ccttccgcca ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg
    37741 atgttcgcca agctgttgca gatactcttt cagctccgca tctgcatggg aacggctgat
    37801 aaccaccagc ggcgggcgcg catcgcggac ctgaatctgc ttgcgcacac cgcactcttc
    37861 tttccaggcc ttgccttctg ccgcgctgta cattatgttc attaccggcg catacaccac
    37921 acctaaaatc ggtttgccat ggtcaatgag cgcaatgtta acggtgaatt cgccattacg
    37981 tttaataaac tctttagtac catccagcgg gtctaccagc cagtaacgct gccagtgctg
    38041 acggacttcc caaccgggag gatcttcttc agaaaggacc ggaatatccg gtgtcagcgt
    38101 acgtaaaccg tccatgataa cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt
    38161 gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg tagacctgca taatggcatc
    38221 gcctgcattc cgtgcaagct ggcatacttg atctaacatt tctccacctc gtctctgtga
    38281 gcggtgttaa cttattgttt tacttatacc ctatcgttaa tgaatgcgcc aactgtgata
    38341 gtgtcatcat tttcaaagcg taaaattgtg gcattcttca ctgttctata agtaaggcgt
    38401 ttattctccc ttttctttcg tattcccgat gataaaagga tgtccctgat gattaagttt
    38461 agcgcaacgc tcctggccac gctgattgcc gccagtgtga atgcagcgac ggtcgatcta
    38521 cgtatcatgg aaaccactga tctgcatagc aacatgatgg atttcgatta ttacaaagac
    38581 accgccacgg aaaaattcgg actggtacgt acggcaagcc tgattaacga tgcccgcaat
    38641 gaagtgaaaa acagcgtact ggtcgataac ggcgatttga ttcaggggag tccgctggcc
    38701 gattacatat cggcgaaagg attaaaagca ggtgatgttc atccggttta taaggcgctg
    38761 aatacgctgg attatacggt cggtacactc ggcaatcatg aatttaacta cggtctggat
    38821 tacctgaaaa atgcgttggc gggagcgaaa ttcccttatg taaatgccaa cgtcattgac
    38881 gccagaacca aacagccaat gtttacaccg tatttaatta aagataccga agtggtcgat
    38941 aaagacggaa aaaaacagac gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc
    39001 atgggctggg ataaagctaa tttatccgga aaagtgacgg tgaatgatat taccgaaacc
    39061 gtgcgcaaat acgtgcctga aatgcgcgag aaaggtgccg atgtcgttgt cgttctggcg
    39121 cattccgggc tatctgccga tccgtataaa gtgatggcgg aaaactcagt ttattacctc
    39181 agtgaaattc ctggcgttaa cgccattatg tttggccatg ctcacgccgt tttcccaggt
    39241 aaagattttg ctgatatcga aggggctgat atcgcgaaag gcacgctgaa tggtgttccg
    39301 gcggtaatgc cgggcatgtg gggcgatcat cttggggtgg tcgacttaca actcagtaat
    39361 aacagcggta aatggcaggt gacgcaggcg aaagcggaag cacggccgat ttacgacatc
    39421 gccaataaaa aatccctcgc ggcggaagac agcaagctgg tagaaacact caaagccgat
    39481 cacgatgcca cacgccagtt cgtcagcaag ccaatcggta aatccgccga caatatgtat
    39541 agctatctgg cgctggtgca ggacgatccg accgtgcagg tggtgaacaa cgcgcaaaaa
    39601 gcgtatgtcg agcattacat tcagggcgat ccggatctgg caaaactgcc ggtgctttca
    39661 gctgctgcac cgtttaaagt tggtggtcgc aaaaatgatc cggcaagcta tgtggaggtg
    39721 gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt atctctaccc caatacgctg
    39781 attgtggtga aagccagcgg taaagaggtg aaagagtggc tggagtgctc cgccggacag
    39841 tttaaccaga ttgatcctga caacacgaaa ccacagtcac tcatcaactg ggatggtttc
    39901 cgcacctata actttgatgt gattgatggt gtgaattatc agattgatgt tacccagcct
    39961 gcccgttatg acggcgagtg ccagatggtt aatgccaatg cggaaaggat taagaacctg
    40021 acctttaatg gcaagccgat tgatccgaac gccatgttcc tggttgccac caataactat
    40081 cgcgcttacg gcggcaaatt tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg
    40141 gatgagaacc gctcggtgct ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa
    40201 attcacccgg cggcagataa caactggcgt ttagcaccga tagctggcga taagaaactg
    40261 gatatccgtt tcgaaacctc tccatcagat aaagccgcgg cgtttattaa agagaaaggg
    40321 caatatccga tgaataaagt cgcgaccgat gatatcgggt ttgcgattta tcaggtggat
    40381 ttgagtaagt aatacacttc tttttcggcc tataaatcat caaccgcatc cggcactttt
    40441 gtcggatgcg atgctggcgc atcttatccg ccctacaagt catgaaccgt aggccggata
    40501 agcgcagccg gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc
    40561 aactcaatcc agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg
    40621 aggctatatt ccacatgcgg cggcaccacc ggatacgcga tacggttaag aaaaccatcc
    40681 tgttctaacg cctgtaacga ctgcgcaagc atcttttcac tcaccccacc gattttgcgc
    40741 cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa caccccccaa
    40801 cggctggtga cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattaccc
    40861 tctttcagtt gttgtgacag gctaacctga ctcatttcat acttaccttt ttgtacgtac
    40921 ttactaaaag taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt
    40981 cccatgatcg ctattactgg tgccactggc caacttggtc actatgttat taaatccttg
    41041 atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag
    41101 gcactgacag cacaaggcat taccgtgcgt caggctgact acggcgatga agccgcactg
    41161 acatctgcac ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa
    41221 cgtgccccgc agcatcgtaa tgttattaat gccgcaaaga cggctggcgt gaaatttatc
    41281 gcttatacca gtctgcttca cgcggataaa tctccgctcg gcctcgccga tgagcacatc
    41341 gagacggaga aaatgttggc tgattctggc atcgtttaca ccctgctgcg caacggctgg
    41401 tacaccgaaa actatctcgc cagcgccccg gcagcactgg agcacggcgt atttatcggt
    41461 gccgcgggcg atggcaaaat tgcctcggca acgcgggcag attatgcggc agctgcggca
    41521 cgcgtgatta gcgaagccgg tcacgaaggc aaggtttacg agctggcggg cgatagtgcc
    41581 tggacgttga cacagttagc ggcagagctg accaaacaga gcggcaaaca ggttacctat
    41641 caaaatctga gcgaaaccga tttcgctgcg gcgctgaaaa gcgttggcct gcctgacgga
    41701 ctggcggata tgctggcgga ttctgacgtt ggcgcatcga aaggcggtct gtttgatgac
    41761 agcaaaacgc tgagcaaatt gattggccgc ccaacgacaa cgttagccga aagcgtaagc
    41821 catcttttta atgttaataa atagttaatt aaagtggcat cctcccgcat cctctctgat
    41881 aatgacggga tgccgggagc aatcatgtct gcttcctgaa cgtttcttct gacagaccaa
    41941 tggatgccag taatgattag cggcgtgctg tacgccctgt tagcagggtt gatgtggggg
    42001 cttatttttg tcgggccgtt gatcgtgccg gaatacccgg cgatgttgca gtcgatgggg
    42061 cgttatctgg cgttagggtt aattgcgcta cccattgcct ggctgggacg cgtgcgtctg
    42121 cgtcagttgg cgcgtcgcga ctggcttacc gccttgatgc tcactatgat gggcaacctc
    42181 atctattact tctgccttgc cagtgccatt caacgtactg gcgcgcctgt ttctacgatg
    42241 attattggca ccctgccggt ggtcattcct gtctttgcca atctgcttta tagccagcgc
    42301 gacggcaaac tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg catcggcctg
    42361 gcgtgtgtga atattgctga gttaaaccac ggactccccg attttgactg ggcacgttat
    42421 acctctggca tcgtgctagc gttagtttcc gtggtctgct gggcatggta tgccctgcgc
    42481 aacgcccgct ggctgcggga aaatcccgac aaacatccga tgatgtgggc gacggcgcag
    42541 gcgctggtca cgctgccggt ttctctcatc ggctatctcg tcgcctgtta ctggctgaat
    42601 atacaaacgc cggacttctc cttacctttt ggcccccgtc cgctggtgtt tattagtctg
    42661 atggttgcga tagccgtgct ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc
    42721 cagcgattac cgacagtgat tctcgggccg ctgatcgttt tcgaaacact ggcaggtttg
    42781 ctgtacacct ttttgatacg ccagcaaatg ccgccgctga tgacgctgag cggtatcgcg
    42841 ctgttagtgg ttggcgtggt cattgcagtc agagcaaaac cggaaaagcc tttaactgaa
    42901 tctgtctcag aaagttgaca tactggcagt gagttaaata agcctctgct acgtaagggt
    42961 tatagcattt gccttaaaga tgcatttaaa atacatctta tcttattaag aatgaggtat
    43021 cagctatggc ttatcgcgac caacctttag gtgaactggc gctctctatc cctcgcgctt
    43081 cggctctgtt tcgtaaatat gatatggatt actgctgcgg cggtaagcaa acgctggcgc
    43141 gtgcggcggc acgtaaagaa ctggatgttg aggtcattga agctgagctg gcaaagcttg
    43201 ctgaacaacc gattgagaaa gactggcgta gcgccccgct ggcagaaatc atcgaccata
    43261 tcatcgtgcg ctaccacgat cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga
    43321 ctaaagtcga gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc
    43381 tgaccatgct gcatgaagag ctttccagcc acatgatgaa agaagagcag atcctcttcc
    43441 cgatgatcaa acaaggcatg ggcagccagg caatggggcc aatcagcgta atggaaagcg
    43501 agcacgatga agcgggcgaa ctgctggaag tgatcaaaca cactaccaat aacgtcacac
    43561 cgccgccaga agcctgcacc acctggaaag cgatgtataa cggcattaat gaactgattg
    43621 atgacctgat ggatcacatc agtctggaaa acaatgtact gttcccacgc gcgctggcgg
    43681 gtgagtaatc taaaagctgg atggcattgc gccatccagc atgataatgc ggttatttcc
    43741 gcagttcagc agcccgcttc ttaccaataa acaaccagcc cagccccagt gcgataaacc
    43801 acagcggggt gaccaacagc gcctggcgag tgtcatcttc cagtgtcagc aacacgacca
    43861 cgaacacaaa gaacgccata catacccagc acatcagctt gccgagcggc atcttgtaga
    43921 tcgacttctc atgcagatga ggacgctgtt tgcggtaaac caggtacgag caaaggataa
    43981 tcgtccagac gaacataaac agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa
    44041 tcacgctagg attcacatac agcatcacca cgccaccgag caggcagatg caggagaagg
    44101 tcagcccttt cgctggtact gcgcgcttag aaagtttagc gaacgctttc ggtgccacac
    44161 cttcctgcgc cagaccaaac agcatacggc tggtagagaa gacgccgctg ttagcggaag
    44221 acgccgcaga ggtcagcacc acaaagttga tcacgcttgc ggcagcaggc agccctacca
    44281 acacgaacag ttcaacaaac gggcttttct ccgggactac cgaactccac ggcgtcacgg
    44341 acataatcac aatcagcgcg aagacgtaga acataatgat acggatcgga atggagttaa
    44401 tcgcgcgtgg cagtgatttc tccggatctt tggtttccgc agctgttgta cctaccagct
    44461 caatccccac gaaagcgaaa accgctatct ggaatccggc aaagaagcca cttaaacctt
    44521 tcgggaacca gccgccgtca ttccacaaat gcgcgaatga cgcttccaca ccggtcggtg
    44581 actgaaagtg catcgccacc atgaccaggc cgacgacaat cagcgacacg atggcgacga
    44641 ttttgatcat cgcaaaccag aactccatct caccgaacat tttcacggtg gcaaggttga
    44701 gcgtcaacag cagcactatc accgccagcg aggcgaccca gtcggagaga tcggggaacc
    44761 agaactgagc ataagcagtg atcgccacca cgtctgccat accggttaca acccagcaga
    44821 accagtaagt ccagccggtg aaatatcctg cccacggccc gagtaaatcg gaagcgaagt
    44881 cactaaaaga tttgtattcc agattcgaaa gcagcaattc ccccattgcc cgcatcacga
    44941 aaaagagcat aaaaccgatg atcatataaa cgaaaatgat cgacggcccg gcaaggctaa
    45001 tcgttttgcc agaccccata aacaaccccg taccaatggc accgccaatg gcaataagct
    45061 gaatatgtcg gtttgtgaga ttgcgccgta gcgactgttc agccggagcc tgatcatcgg
    45121 caacgacttt tacctgatct accatgtttt tttcttcctg tacctgtctg tgttgttcag
    45181 gctctacggc ctttagtcag tctatgatac gacgaatctg tatcgggact catcgatatt
    45241 aggtaacaat acgcgggatg aatactaaga tttagatcta atgttaattt tatgtttaaa
    45301 gtgagtgtca tatcacggtg ataacgcgaa acagctcaca aaaatacacg cttacggggt
    45361 ctttgcaaca caaaatagcg ccgtttggca aactgaaggg tttattgctg aatgcctgct
    45421 cccctctggt tcgaggggaa cagaatgcgc tgcttagagg atttccagca gttcgacttc
    45481 aaacaccagg gtgctgaacg gagggatgga tgcgcctgcg ccgcgctcgc catatgccag
    45541 ttcctgcggg atagtcagtt cccatttaga acctaccggc atcagagtca gtgcttcaat
    45601 ccagccaggg atcacgccat taaccgggaa ttcagcgggt tcaccacgag caacggagct
    45661 gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga acacgaacgc ggtcagtacg
    45721 tgccggaatt gcgccttcac cctggttgat cacgcggaat tgcaggccag attcggtgct
    45781 gttcacacct tcttttttgg cattttcttc aagatatttc acaccttcgg cagccatcgc
    45841 ctggaaacgc tgacgacgaa cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg
    45901 caccacatca accggaacag ccggatgttt gccttccagc gcatctgcaa taccagccac
    45961 cagcgcctcc ggcagcagcc cttgcaggcc agattcactc agttgttggc ctacctgtaa
    46021 accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt
    46081 tcctttctta gggtataaaa gtagcgggca gcatatcagc cacacttctt tgggtaaaac
    46141 tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa caatagcaat atcccgtatg
    46201 aatataataa aggcgggttt accacggaca tcatagcgtt agcttattca taggctatga
    46261 ttgaggaaca agacgcggag caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa
    46321 ccctggagaa agtctggcac gcgccggaca attttcgctt catggacccg ctgccgccga
    46381 tgcatcgtcg ggggatcatt attgccgcca ttatattggt ggtcggattt ctgcttccat
    46441 ctgatgatac gcccaacgcg ccggtggtga cgcgtgaagc gcagttagat attcagtcgc
    46501 aatcacaacc gccgacagaa gaacagctgc gggcgcaatt agtcacacca caaaatgatc
    46561 ctgatcaggt ggctccggtc gctccagaac ctatacagga aggccagcca gaagaacagc
    46621 cgcaaactac ccaaacacaa ccattccagc cagatagcgg aattgataat caatggcgct
    46681 cttaccgtgt cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg
    46741 ctaccgatgt ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc
    46801 aaaacgggca aatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca
    46861 ttgatacggg gaataatcaa caggtcctgt tcacccgcca gccggatggc agttttattc
    46921 gtgcgcggta gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt
    46981 acgtctggct tccagtgcct ggggggaaac catactgata tgataaacca ggccaagcgt
    47041 atgctggttg atcaaataat atccgccaag cgcagcaata ttaatattca cctgcaatgg
    47101 gtcgatatct ggcttaaaaa tattgtgttt cttcccttca tccaataagg attccattaa
    47161 ctgcaagtgc gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt
    47221 actttgattt tcactatgca caatttttaa aaaccaggga ttagtaatat aataatccca
    47281 tgtaaaatca ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc
    47341 cttttctttt tgtcgaatat cgttccaggt atattccagt acttcaataa ataaattttc
    47401 tttattacca aagtgatgat aaaccgtctg tttactacac cccgcagcct taacaatatt
    47461 atcgaccctg gccccttcat aaccatattc ggcaaattca ttaaccgcgc ataacagcag
    47521 cttttccttc aggctctgta cgttgctggt atcaagatac ataacgcttc cccacgacct
    47581 cttcaagata actatcttaa cccgattaat tagaagctcc agtaaaaagg ataaaaactt
    47641 gtactttcat caagttatca aatgtcctgt gatcactatc gcagatcaac caaccagatg
    47701 gttgatcttt cttgcggcac gtcgcaagct aattgtcact tagaaaacaa tattcaaaaa
    47761 aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt gattaccgga gccgctcgtg
    47821 gtattggaaa gggctgtgcg ctggagcttg ctcgtggcgg atttaacctg ctgattaacg
    47881 atcttcccga tgccgacagc gtagaaaaac tgcatatcac acaacaagaa tgtatagctg
    47941 aaggcgtgga agtgatttgc ttccctgccg atgtcggcga tctctccttg catgaagaga
    48001 tgctcgacgc ggcgcaaaac ctgtggggac gtctggactg cctgctcaac aacgcaggca
    48061 tttcagtgaa aaaacgcggt gacctcctcg acctcgaacc agacagcttc gatcaaaaca
    48121 ttgccattaa cacccgcgcg ccgttcttcc ttgctcaggc attcagtaag cgcttactgg
    48181 cacagccgaa accggaagcg gagctgccgc accgctcgat tattttcgtc agttccatca
    48241 acgccatcat gctggcgatg aatcgcggcg aatacaccat tgccaaaacc gccgtttctg
    48301 ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca aattggcgtt tatgaagtgc
    48361 gccctggtct catcaaaacc gatatgacta ttccagcaac ggcttattac gacgaactga
    48421 ttgccaaagg tctggtgccg tggggacgtt ggggttatcc ggctgatatt gcttccaccg
    48481 tccgcgcgat ggcggaaggt aaactgattt acacctgtgg tcaagcggtc gccatcgacg
    48541 gcggcttaag tatgccgcgc ttctgaggct gaatcatgag agatctgtta caacgcccgg
    48601 atttattcag tatcaatacc gcgacactgg gctataaaac gccgctaccc gccattattg
    48661 atgcctgcgc cgcacgcggt attggcgcaa ttgccccctg gcgcagggag ttgcagggtg
    48721 aagatctcca gcaaatcgcc cgccagcttg ccgccagtaa catgagcgtt tccggtttgt
    48781 gccgtagcac ttactacacc gcgccgacac tggcggaacg caaactggcg attgatgata
    48841 accgccgggc gctggacgac gcagcggtcc ttaacgccgc ctgttatatg caggtggttg
    48901 gcgggttacc gcagggaaca aaagatttgt acgaagcgcg ggaacaagta aaacaaggga
    48961 ttcgccagtt gctgccacat tcgaaagatg tcggcgtgcc aattgcgctg gagccgctgc
    49021 acccgatgac tgctgccgac cgctcttgcc tgtgcacgtt acgtcaggcg ctggactggt
    49081 gtgatgaact cgatcccgat ggtgagtttg gcctcggcgt ggcggtggat gtttatcacg
    49141 tctggtggga tccggagctc gccagtcaga tcctgcgtgc cggaaaacgc atcctcgcgt
    49201 ttcatgtttc cgactggtta gtaccgacca ccgatctggt caatgaccgg ggaatgccgg
    49261 gagatggcgt catcaatatt ccgtcaattc gtcggctggt tgaaaacgcc ggatttaatg
    49321 gcgctataga actggaaata ttctcgcctt actggtggca gaaagatatt aacagcacgc
    49381 tggatattag cgtcgatcgc atcgcgcatt attgttaagg aatgatcatg agtgaccaac
    49441 ccgttctgtt cagcagagcg gcggcttctt gccgcctgac attgaaccgt gaagataaat
    49501 gtcatgctat taatgaagaa atgatcgaat ctctcgacca ttatttaaat gaaattgaaa
    49561 acgacaccac attacgatta gtcgaattaa cggcaaccgg cgataaattc ttttgtgcgg
    49621 gtggcgatat taaatcgtgg tcggcctatt cgccattaga tatgggcaga aaatggatta
    49681 aacgcggtaa tgacgttttt aatcgcctgc gaaacttacc ccaattaacc gttgcgaatc
    49741 tcaacggtca taccatcggc ggtggtattg aactggcctt gtgctgcgat attcgtattg
    49801 cccgccccgg cgcgaaattc tctaacccgg aagtgatgct cggcatggtg cctggctgga
    49861 tgggcattga acgtgtgctt aatcaggttg gtccggtggt cggtcgccag atgctgatgc
    49921 tgggtaaacg cctgacggcg caggaagccc aggcggcgaa tttaattgat gaagtggtag
    49981 aaaaagaaca ggtggaaagc tggatggcga accagttagc gcagctggaa aaatgcggtc
    50041 cggtggcact ggcgcatatt aagcaactga ttctggcact ggaaaataaa catgccgatt
    50101 acccgcacca gctactggcc ggattaatgt ctgccactca ggattgccag caggcgacgc
    50161 gcgcctttgc cgaaaaaagt agcgtgagct tccataatca ataaggaaat tgtatgcgta
    50221 agataacaac cgccgaggca ctggcggcgc aaattcagga cggtgcgact attgctatta
    50281 gcggtaacgg cggcggtatg gtagaagccg accatattct ggctgctatt gaagcgcgtt
    50341 tcctgcaaac cggacaccct cgcgacctga cgctaatcca ctcgctgggt attggcgatc
    50401 gcgactgcaa aggcactaat cgttttgcgc acgcagagat gctcaaacgc attattgccg
    50461 ggcactttac ctggtcgccc aaaatgcagg cgctggtaaa aaataacacg attgaagcct
    50521 actgctttcc tggtggcgtg attcaggcac tgctacggga gatcggcgcc ggacgtccgg
    50581 ggctttttac ccacgttggg ctgggatcgt ttgttgatcc acgcaatggc ggcggtaagt
    50641 cgaatgaatg cactaccgac gagctggtag aactgattga aatcgacggt gaaaccaaac
    50701 ttcgttatcg ccctttcaaa gtggattacg cgattttgcg tggcacttat gccgatcctc
    50761 gaggcaacgt cagccttgaa gaagaagcga ttgatatgga tagctattcc atggcgctgg
    50821 cagcacacaa cagcggcggc aaagtgttcg tacaggtacg cgatgtgctg gaagctggcg
    50881 cgattgaacc acgccgggtt aaattaccgg gcattctggt tgatggcatc gttgaacacc
    50941 gcgaacagcc acaaacctat cttggcggtt acgacctgac catcagcggc caacatcgcc
    51001 gtctgagttc taacgacgct attgaactgg ttagtcatcc ggtacgtcgc ctgattgccc
    51061 gtcgggcagc acgcgaactg gtagctggcg cttcaaccaa ctttggcttt ggtattcccg
    51121 gcggcattcc aggagtagcg ctgcgcgaag gcgttcctta ccaaagttta tggctgagcg
    51181 tcgagcaagg cgtacataac ggcatgatgc tggatgatgc gtttttcggc tgcgcccgca
    51241 acgccgatgc cattattcca tcactcgatc aatttgagtt ctacagtggc ggagggatcg
    51301 atatcacctt cctcggcatg ggagagatgg atcagtacgg taacgtcaac gtctcccatc
    51361 tcaatggcaa tctgattggc cccggcggat ttctcgaaat tgcgcaaaat gcccgtaaag
    51421 tggtgttctg cggcacgttc gacgctaaag gcagcaagat cgatataaca ccagatggct
    51481 tgcatatcgc ccagtcaggt caaatcccta aactggttac caaggtggaa aaaatcactt
    51541 ttagcgccgc ctacgcacag caaagtggtc aggaagtgtt gtatatcact gaacgcgcag
    51601 tattccagtt aacggcagaa ggcgttgaat taattgaaat cgcgccgggt gtggagattg
    51661 agcgcgacat tctgccgtat atggctttcc gtccaattat caagcaccca cgcctgatgg
    51721 aaagtagcct gtttacgccg atggaggatg catgagtcag cttgatgaca ccatccttga
    51781 tgcgctgacg cacgttactt tcccaaaggg ttttgcacag gcagagcccg catgggttgt
    51841 cacggtagac ggtgttgatt acccactctg gcaaacagat gccctggtag tcggcagtgg
    51901 tgcagccggg ctgcgtgcag ctgtggaact taaacgccgc cagcaaaatg tgctgatcgc
    51961 caccgccggg ttatatatgg ggacgtcggc atgttctggt tccgataaac agacactgtt
    52021 taccgccgct accgcgggca acggcgacaa cttcaccaaa ctggcagagg cactggcgag
    52081 cggtggggcg atggatcacg acaccgctta tgtcgaagcg gtaggttctc tgcacactct
    52141 tggcgggttg caatatcttg gtctggaatt accggaagat cgctatggcg cgattcttcg
    52201 ttatcaaacc gaccatgacg aagccgggcg tgcaacctcg tgtgggccgc gtacctcaag
    52261 gttaatggtg aaagtgctgt tggaagaagt acagcgcctc gccattccag tgttgaccag
    52321 tgcaacagtg attaaactgc tgcatcagcg tgacgaaaac ggcgaagacc gtgttgcggg
    52381 ggcaatcctc gcgaccggtc atcgcgccca taacccttgg gggctggcaa ttgtgactgc
    52441 gcccaatgtg gtactggcaa caggagggcc tggcgagctt tatcgcgaca gtgtgtaccc
    52501 acacaaatgt tttggctcgc tggggctggc gctggaggaa ggcctgacgc taaccaatct
    52561 gaccgaaagc cagtttggta taggcacgcc gcgtagcacg tttccgtgga atttatccgg
    52621 cacctatgta caggtgatcc cgtatatcta ttccgtggat gctgagggta acgagtataa
    52681 cttcctcgcg gattactatc gcaccaccca ggagctggct tcaaacattt tccgtaaagg
    52741 ctatcagtgg ccgttccacg ccactcgggt gatggatttt ggctccagct tgttagatat
    52801 ggcagtagcg caagagcagc aatcagggcg tcaggtattt atggatttca atcgcaatcc
    52861 tgaacctgtg ccgggtgacc tgccattctc attagagcga ctggacgacg acgttcgcgc
    52921 gtatctggaa aataacgacg ctctggcacc atcgcccatc gaacgactgc aacgaatgaa
    52981 tccgctgtct atctcgctgt ataagatgca cggttacgat ctcaccacgc agccattgca
    53041 gtttgccatg aataatcagc acatgaatgg cggcatttaa gtggatatct ggggacaaac
    53101 atccctgccc ggttgttttg ccgtggggga agttgctggc acacacggcg tcactcgccc
    53161 tggtggtgcg gcattgaatg ccgggcaggt ttttgctgtt cgtctggcac gttttattgg
    53221 ttgcacgcaa aaacgtaata ttgatggaga tatagcacag ctggtagctc aggcactggc
    53281 ttctataaga gagatcatta ctcaggcgca cgataacggg accgggatgc cgttgtcggt
    53341 tgtgagagaa aaaattcagg cacgaatgtc tgaccatgcg ggatttattt gccatgccga
    53401 taaagtccga cgcgccactc gtgatgccct gctattgaac gaatttgtcc aacggcatgg
    53461 attggctatc aaacatgtgg gcgaagttgc cgagctgttt atgtggcggc atatggcgct
    53521 gacctctgcc gccgtcttaa ctcaactgac acattatatt gatgctggtg gtggcagtcg
    53581 tggggcacgg atagttattg atccacaagg caaatgccta ccacaaactc gtcgcggcgc
    53641 aaaagaagaa tggcgttttc gctctgaacg tgctgaagac aaaaatcaca gattaacgat
    53701 tcaatattcg caaggttctt ttattaccga agtgaagtct ttacgtatgc aaccgtgtat
    53761 taacggtatt tactttgaaa aaaactggcc agacttttta aatggagaaa tttacacaca
    53821 ataattttca atatttcctc tgtacctgat ttttattacc tattacacgg aaacaccaac
    53881 aaccagggtt tattaatata attaaaaggt ttaaacatga ataaaataaa gaattaccgg
    53941 tggcatatga ttgccctcgt atgctttatc actgtaatca attatctgga cagaacggca
    54001 ttaggtattg cggctccaac gattatggag acaaccggaa taactaaaga gcaatattca
    54061 tggattgtca gtgcattcca gttggcctat acattagggc aaccgg
//
LOCUS       DAFAVF010000015        51994 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFAVF010000015 DAFAVF010000000
VERSION     DAFAVF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.015"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..632
                     /locus_tag="LGK42_000864"
     CDS             <1..632
                     /locus_tag="LGK42_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2657141.1"
                     /translation="RRSREIVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGV
                     IDNLFDRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQEL
                     PWLQMRFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAW
                     RLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(629..2107)
                     /gene="scpC"
                     /locus_tag="LGK42_000865"
     CDS             complement(629..2107)
                     /gene="scpC"
                     /locus_tag="LGK42_000865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBK2657142.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2131..2916)
                     /gene="scpB"
                     /locus_tag="LGK42_000866"
     CDS             complement(2131..2916)
                     /gene="scpB"
                     /locus_tag="LGK42_000866"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBK2657143.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2927..3922)
                     /gene="meaB"
                     /locus_tag="LGK42_000867"
     CDS             complement(2927..3922)
                     /gene="meaB"
                     /locus_tag="LGK42_000867"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBK2657144.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPSHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3915..6059)
                     /gene="scpA"
                     /locus_tag="LGK42_000868"
     CDS             complement(3915..6059)
                     /gene="scpA"
                     /locus_tag="LGK42_000868"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBK2657145.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLESVRDVLNLISQHHD"
     gene            complement(6263..7156)
                     /gene="argP"
                     /locus_tag="LGK42_000869"
     CDS             complement(6263..7156)
                     /gene="argP"
                     /locus_tag="LGK42_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBK2657146.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7298..7483
                     /locus_tag="LGK42_000870"
     CDS             7298..7483
                     /locus_tag="LGK42_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657147.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            7539..8198
                     /gene="rpiA"
                     /locus_tag="LGK42_000871"
     CDS             7539..8198
                     /gene="rpiA"
                     /locus_tag="LGK42_000871"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK2657148.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8454..9686
                     /gene="serA"
                     /locus_tag="LGK42_000872"
     CDS             8454..9686
                     /gene="serA"
                     /locus_tag="LGK42_000872"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK2657149.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9915..9974
                     /gene="ibsC"
                     /locus_tag="LGK42_000873"
     CDS             9915..9974
                     /gene="ibsC"
                     /locus_tag="LGK42_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBK2657150.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10307..10366
                     /locus_tag="LGK42_000874"
     CDS             10307..10366
                     /locus_tag="LGK42_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2657151.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10467..11069)
                     /locus_tag="LGK42_000875"
     CDS             complement(10467..11069)
                     /locus_tag="LGK42_000875"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK2657152.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11090..11273)
                     /gene="ssrS"
                     /locus_tag="LGK42_000876"
     ncRNA           complement(11090..11273)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LGK42_000876"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11315..11644)
                     /gene="zapA"
                     /locus_tag="LGK42_000877"
     CDS             complement(11315..11644)
                     /gene="zapA"
                     /locus_tag="LGK42_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK2657153.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11812..12390
                     /gene="ygfB"
                     /locus_tag="LGK42_000878"
     CDS             11812..12390
                     /gene="ygfB"
                     /locus_tag="LGK42_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBK2657154.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSGTSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12416..13741
                     /gene="pepP"
                     /locus_tag="LGK42_000879"
     CDS             12416..13741
                     /gene="pepP"
                     /locus_tag="LGK42_000879"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBK2657155.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13738..14916
                     /gene="ubiH"
                     /locus_tag="LGK42_000880"
                     /gene_synonym="visB"
     CDS             13738..14916
                     /gene="ubiH"
                     /locus_tag="LGK42_000880"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBK2657156.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREELLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14939..16141
                     /gene="ubiI"
                     /locus_tag="LGK42_000881"
                     /gene_synonym="visC"
     CDS             14939..16141
                     /gene="ubiI"
                     /locus_tag="LGK42_000881"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBK2657157.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWSKAQQASDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSSALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16215..16298)
                     /locus_tag="LGK42_000882"
     CDS             complement(16215..16298)
                     /locus_tag="LGK42_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657158.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            16588..17682
                     /gene="gcvT"
                     /locus_tag="LGK42_000883"
     CDS             16588..17682
                     /gene="gcvT"
                     /locus_tag="LGK42_000883"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBK2657159.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17706..18095
                     /gene="gcvH"
                     /locus_tag="LGK42_000884"
     CDS             17706..18095
                     /gene="gcvH"
                     /locus_tag="LGK42_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBK2657160.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18214..21087
                     /gene="gcvP"
                     /locus_tag="LGK42_000885"
     CDS             18214..21087
                     /gene="gcvP"
                     /locus_tag="LGK42_000885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBK2657161.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21348..22091
                     /locus_tag="LGK42_000886"
     CDS             21348..22091
                     /locus_tag="LGK42_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2657162.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22148..23581)
                     /gene="bglA"
                     /locus_tag="LGK42_000887"
     CDS             complement(22148..23581)
                     /gene="bglA"
                     /locus_tag="LGK42_000887"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBK2657163.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23626..23937
                     /locus_tag="LGK42_000888"
     CDS             23626..23937
                     /locus_tag="LGK42_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK2657164.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTITLETLTEKHAEQENMTLPELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24101..24760
                     /locus_tag="LGK42_000889"
     CDS             24101..24760
                     /locus_tag="LGK42_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK2657165.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24837..25817)
                     /gene="ygfZ"
                     /locus_tag="LGK42_000890"
     CDS             complement(24837..25817)
                     /gene="ygfZ"
                     /locus_tag="LGK42_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBK2657166.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26060..26326
                     /gene="sdhE"
                     /locus_tag="LGK42_000891"
     CDS             26060..26326
                     /gene="sdhE"
                     /locus_tag="LGK42_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBK2657167.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26307..26714
                     /locus_tag="LGK42_000892"
     CDS             26307..26714
                     /locus_tag="LGK42_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBK2657168.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26754..27275)
                     /gene="fldB"
                     /locus_tag="LGK42_000893"
     CDS             complement(26754..27275)
                     /gene="fldB"
                     /locus_tag="LGK42_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBK2657169.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27387..28283
                     /gene="xerD"
                     /locus_tag="LGK42_000894"
     CDS             27387..28283
                     /gene="xerD"
                     /locus_tag="LGK42_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK2657170.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28308..29018
                     /gene="dsbC"
                     /locus_tag="LGK42_000895"
     CDS             28308..29018
                     /gene="dsbC"
                     /locus_tag="LGK42_000895"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBK2657171.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            29024..30757
                     /gene="recJ"
                     /locus_tag="LGK42_000896"
     CDS             29024..30757
                     /gene="recJ"
                     /locus_tag="LGK42_000896"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK2657172.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            30848..31946
                     /gene="prfB"
                     /locus_tag="LGK42_000897"
     CDS             join(30848..30922,30924..31946)
                     /gene="prfB"
                     /locus_tag="LGK42_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK2657173.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            31956..33473
                     /gene="lysS"
                     /locus_tag="LGK42_000898"
     CDS             31956..33473
                     /gene="lysS"
                     /locus_tag="LGK42_000898"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK2657174.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33516..34064)
                     /gene="idi"
                     /locus_tag="LGK42_000899"
     CDS             complement(33516..34064)
                     /gene="idi"
                     /locus_tag="LGK42_000899"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK2657175.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            34119..34190
                     /locus_tag="LGK42_000900"
     CDS             34119..34190
                     /locus_tag="LGK42_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657176.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34187..34312)
                     /locus_tag="LGK42_000901"
     CDS             complement(34187..34312)
                     /locus_tag="LGK42_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657177.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34314..35762)
                     /gene="uacT"
                     /locus_tag="LGK42_000902"
     CDS             complement(34314..35762)
                     /gene="uacT"
                     /locus_tag="LGK42_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBK2657178.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            36198..38117
                     /gene="ygfT"
                     /locus_tag="LGK42_000903"
     CDS             36198..38117
                     /gene="ygfT"
                     /locus_tag="LGK42_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBK2657179.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNACEEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            38117..38605
                     /locus_tag="LGK42_000904"
     CDS             38117..38605
                     /locus_tag="LGK42_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBK2657180.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(38641..40008)
                     /gene="ghxQ"
                     /locus_tag="LGK42_000905"
                     /gene_synonym="ygfQ"
     CDS             complement(38641..40008)
                     /gene="ghxQ"
                     /locus_tag="LGK42_000905"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HBK2657181.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(40044..41360)
                     /gene="guaD"
                     /locus_tag="LGK42_000906"
     CDS             complement(40044..41360)
                     /gene="guaD"
                     /locus_tag="LGK42_000906"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBK2657182.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(41378..42778)
                     /gene="xanQ"
                     /locus_tag="LGK42_000907"
     CDS             complement(41378..42778)
                     /gene="xanQ"
                     /locus_tag="LGK42_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBK2657183.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(42943..45813)
                     /locus_tag="LGK42_000908"
     CDS             complement(42943..45813)
                     /locus_tag="LGK42_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBK2657184.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(45810..46589)
                     /locus_tag="LGK42_000909"
     CDS             complement(45810..46589)
                     /locus_tag="LGK42_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBK2657185.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(46640..47968)
                     /gene="ssnA"
                     /locus_tag="LGK42_000910"
     CDS             complement(46640..47968)
                     /gene="ssnA"
                     /locus_tag="LGK42_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBK2657186.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(47971..51069)
                     /gene="ygfK"
                     /locus_tag="LGK42_000911"
     CDS             complement(47971..51069)
                     /gene="ygfK"
                     /locus_tag="LGK42_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBK2657187.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPVGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(51391..51969)
                     /gene="mocA"
                     /locus_tag="LGK42_000912"
     CDS             complement(51391..51969)
                     /gene="mocA"
                     /locus_tag="LGK42_000912"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBK2657188.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
BASE COUNT        12565 a        13138 c        13797 g        12494 t
ORIGIN      
        1 ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca attattttcg
       61 atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca gttgagttca
      121 gcgaaaacgg tgttatcgat aatctttttg accgtcagtt agattttatt atctccccac
      181 agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa ttaccaccgc
      241 tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa gagctgcttc
      301 aggagctgcc ctggctacaa atgcgctttc aaaaccgggc taacttcgaa gcaatgattg
      361 atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc tacagtttta
      421 tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg cattttgcct
      481 ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg ccgatgtata
      541 tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg cttcagcata
      601 ttgctgaaga tcgtgacggg acaaaccatt aacccagcat cgagccggtt gcaattaaat
      661 tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgccg ggcgcatttt
      721 ccagatagcg atgcagataa tcccgataca taggatgtgc acaattatca atgatagtgc
      781 gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg gtgatgatca
      841 ctttgacgct gtgttcgctg tgatcaacat ggctgcacat tggcacgacg gttgagatct
      901 tgccttcttt agcaatcgac ggagccataa agatcgacag atacgcgttg cgttcaaaat
      961 caccgctgcc gccgatgccg ttcatcagat cgaccccggc tacgtgcgtt gagttggcat
     1021 gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga cgacggatga
     1081 tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca aagtaatcca
     1141 tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag gcgctggccc
     1201 cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact tccgaataca
     1261 tcatgaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca ttattgatat
     1321 tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc gccatttcct
     1381 gcaataagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt tgcttatcca
     1441 gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt ttcggatcga
     1501 tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg ctattgcgcc
     1561 gcggtggcgc gccaggaatc acaatatctg ccagttctgc aacgcgcgga tcgtgatagt
     1621 gattaagttc aatgatcact ttcttcgctc gcagcagcca ggtcggcgca ttaccgatcc
     1681 cgctggttaa ccagactcga ccatccggtg ccagtgccga tgcttcaatg acggcaacat
     1741 caatgtcgcc gaagaaaccg taattgacca tttgcgccac ttcgctcaaa tgcaggtcaa
     1801 cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt tgatatggcg
     1861 cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc agcggcggcg ctgattgacg
     1921 cccccgtcag caggcgaatt tgatacggct ttttggcctc atgctgttcg ttagctctgc
     1981 gggcaatcgc ggtgggtagg gctttcggcg aacccgccgg ggtaaagccg ctaaatgcca
     2041 ccatgtcgtt atgctggata atttctgccg cttcatcggc ggtcatcctt gtccactgag
     2101 tttccatttt tactccttcg ttcgcaggga ttaatgacca acgaaattag gtttacgttt
     2161 ttcgaggaaa gcgttcattc cttcctggta atcttcgctg tcataaaccg cgcgtcgcat
     2221 cccctgaata cgttcaaatt catcggagtt catggtgtgc gcttcgccca gtacacgcag
     2281 ctcttctttg ataacagcaa tggctaacgg cgctttctca gagatgtggt gcgccatttg
     2341 taaggtgaaa tcttccagtt cttccacttc cacaacatgg ttgaggatgc cgacagccaa
     2401 cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagt tctttgacaa tgtggaagcc
     2461 cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga ggtttacagg
     2521 cgtcattgag aaggttgagg tactggcggc gataatcaga tcggaactca tgatcatttc
     2581 aaatgcgcca ccccaaacac taccttccac catcgaaatg atcggtttcg ggaatttttg
     2641 gatcatgcgg gtgatttgac gcaatggatc atcataggag agcggatcgc gaccgccaga
     2701 cggcagttcg tgaatatcgt ggcctgcgga gaagactttg gatccactcg gcgcgcgcaa
     2761 aatgatacag cgaatttccg gccggttgag atcgcttaat gcctgcataa gatcatcaat
     2821 aaagacttta cttaaggcat taagtttgcg gccatagtta aactcaatga ccgccacttt
     2881 gttgatagtg acaacgttaa catactgata agacataaaa attcctttaa tcaaaatatt
     2941 gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc gtattgtttt
     3001 tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc ttcattcgcg aacaggtgat
     3061 gcagtacttc ttcttcggtc tgcttacgca gccattccac cgattgttgt tgccgcactt
     3121 gttgtaaacg accactggca gttagcgcgg ttttgaagtc gatgatggcg tgccagatct
     3181 catcgattcc acgtttttcc agtgcgctac aagtcagaac ccgtggctgc cattcgtcgt
     3241 atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg gcgacattgg
     3301 tatggttatc gccatcgtct ttgttgataa cgagcagatc agccacttcc atcagccctt
     3361 ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata aaacagtcca
     3421 ccatgcgggc gacttccgtt tccgactgcc cgacgccaac cgtttcgaca atcactacgt
     3481 cataacccgc cgcttcgcat aacagcatta attcccgcgc tcgctgactg gcaccgccca
     3541 gatgaccgga ggatggtacc gggcgaataa acgccgcttc ggcacgcgcc aggtcattca
     3601 tgcgggtttt atccccgaga atgctaccgc cagtgaccgg gctgctggga tcgaccgcaa
     3661 taaccgcgac ctttaatccc tcacgaatca acaacatgcc aaaggcttca agaaaggtac
     3721 ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag ggcgttaccg cagtacggca
     3781 taatggcatc aagcagttgc gtacttagcg cctgatgact cgggtgacgg ctttccacca
     3841 gcgtcatggc ctgggcgagt gtggcacgct caccctgacg taagcggcga atactttctg
     3901 ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc gcgcacactc
     3961 tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg caggaaggcg
     4021 taatcctgcg gtggaatgac gccaccggcg actacgcaga tatcttcgcg tccccatttt
     4081 ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag tgaggatgcg
     4141 cccactacgt gaacgtcgtt ttctacggcc aggcgggcga tctcttcagg tgtagagaac
     4201 atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat cactttcgcg
     4261 ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa tgcgcgggcg acgaccattg
     4321 tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc cgatttctca
     4381 gactgatgat agctttgcgc aatcacgccg gtaacacact ggcttggcac cagataacgg
     4441 tcgaaagcga cttccagcgc atcggaaatt tcacccaggg tggcgcgaac gcgagcggca
     4501 ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag ggcgttcaac
     4561 gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga agcaatttgc
     4621 tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc gtgatccagt
     4681 ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga ctgttcgcgc
     4741 gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt cgccatgcca
     4801 ccggcttcgt cgatctgttg gataatagct ctggcttgtt tgacgatttg atcggtcagc
     4861 gactcaatgt aataggatcc ggccagtgga tcgacggtgc ggcagagttc tgattcttcc
     4921 tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg caaaccaagc
     4981 gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc agccagcgct
     5041 tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct ccagcctgag
     5101 gtctggcagt gggtacgcag cgccagtgat ttcggatcct gtgcgccaaa tccactgacc
     5161 gcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa cagatccatg
     5221 ccgataccga agaagaacga caggcgagga gcgaagtcat caattttcag tccggcagag
     5281 attgctgctt tgatgtactc aatcccatca gcgagcgtaa atgctacctg ctgcacgcag
     5341 ttggcacccg cttcacccat gtggtaaccg ctgatactga tggtattaaa tcgcggcatg
     5401 ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt tggcgggtaa
     5461 atataggtgt tgcggcagag gtactctttg aggatatcgt tctgaatggt gccggtcagt
     5521 ttatcaggtg taacaccttg ctcttctgcg gcgacgatat aaaacgccag tactggtagc
     5581 actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg gtcgaacagg
     5641 actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc acccgccacg
     5701 cgtgggttat cggagtcgta gccacggtgg gtggcaaggt caaacgcaac ggaaagacct
     5761 ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc tgttgaaaaa
     5821 ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg cgggccacga
     5881 acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag atcggcttcg
     5941 gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga gtcgacagtt
     6001 ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgctc gttagacatt
     6061 atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cttacggtga gctttttacg
     6121 tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc ctgagatttg
     6181 tctattttgt gatgtgattc aaggaataca aaaagaagtg aggataacgc ctgatatgtg
     6241 ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca taatcgagca
     6301 acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc cagtagagca
     6361 tccggcgttg aaatagcccc ggcgttaagt caatcagttc accgctggcc agttctttct
     6421 cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt tgtacgaacg
     6481 cttctgaaga attaacgata tggcagggca cgctgcctgg aggcagatcg aagttttgct
     6541 gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc gctttcagta
     6601 atgccgaacg cgttacgccg ttagggaaat atttttcggc aaagggtttt gagctgacga
     6661 acagatagtc gagcgcacca agtttatcga caagacagct cggcagcgcc tgatgttgaa
     6721 tactcaccgc gccgaccact tcgccgcggc gcagacgttc ctgagtgcgg gtttcatctt
     6781 ctacctgcaa gttgaggcgg ataggcgaat cagccaacac aggagccagt gcaggaagca
     6841 accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag cagcagcgga gtcgaaccgg
     6901 tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc agtgccagca
     6961 gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc ggctgcccga
     7021 acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg cacagctttt
     7081 gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt aatgttctgt
     7141 agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct gcactatgac
     7201 acaattttgt gtcaggttgc atataccgaa gccgtcggcg ggtaacattt gtattcgtgg
     7261 ttaaacgcgc agcttaaaag atcagggatt tgcgctaatg catttcgctc agcgagttcg
     7321 cgccctggtt gtgttgaatg gcgtggcgtt attgcctctg ttcgcaccgt ggagcggcat
     7381 acccagaccg ttgtatgctt tatttgcggg gcgaaagggg atgcccgcca ttgcgcgata
     7441 ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt tttttgtata
     7501 taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat gaattgaaaa
     7561 aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt ggtgtaggta
     7621 caggttccac cgccgcacac tttattgacg cgctcggtac aatgaaaggc cagattgaag
     7681 gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc attcacgttt
     7741 ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat gaaatcaacg
     7801 gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt
     7861 cggttgcaga aaaatttatc tgtattgcag acgcttccaa gcaggttgat attctgggta
     7921 aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg cgtcagctgg
     7981 tgaaactggg cggtcgtccg gaataccgtc agggcgtggt gaccgataat ggcaacgtga
     8041 tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac gccataaatg
     8101 cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac gttgcgctga
     8161 ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga acctcccccg
     8221 ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgcttttacc aggcaattgt
     8281 cgattgctct aaataaatcc tctaaaccag catattcatc caagaattac ctttgcgtga
     8341 tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt tttgatatgt
     8401 tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga caggattggg taaatggcaa
     8461 aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg
     8521 cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa ggcgcgctgg
     8581 atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta
     8641 cccatctgac tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt ggctgtttct
     8701 gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta
     8761 acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc
     8821 tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg aacaaactgg
     8881 cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg
     8941 gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg
     9001 aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata
     9061 tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat atgatgggcg
     9121 cgaaagagat ttcgctaatg aagcccggct cgctgctgat taatgcctcg cgcggtactg
     9181 tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatctggcg ggggcggcaa
     9241 tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct ccactgtgtg
     9301 aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga
     9361 atatcggcct ggaagttgcg ggtaaattga tcaagtattc tgacaatggc tcaacgctct
     9421 ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg tggtcgtcgt ctgatgcaca
     9481 tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatcttcgcc gagcagggcg
     9541 tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat gggttatgta gttattgata
     9601 ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta
     9661 ccattcgcgc ccgtctgctg tactaattcc ccttctctga aaattaacgg gcaggtcact
     9721 gacttgcccg tttttttatc ccttctccac accgttgccg cgttgcgcat tctactttgc
     9781 gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga
     9841 tttacgatgg cgggacagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
     9901 aaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta ctgataagtt
     9961 tcggagctta ttaacagtca gtctcagggg aggagtcatc ctcccttacc cttactcact
    10021 aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg cggggtgcat cgccctgaac
    10081 cgaacttttt gatagccact tcatcgccca cgtttactgc cgtaatagat cctttttccc
    10141 aaaaattatc tctgttttgc atggggtttt tcgagcacgc tcgaagaaaa gatttaaagg
    10201 tagatcaatg cgttgtggtt atttaaagag agcttacgat agctgggcag catggggctg
    10261 taacggtaaa gccctcaccg aagcgagggc ttgaaggaga agggttatga tgcgacttgc
    10321 catcatactg attgtactgt tactcataag tttcccagct tattaacagt cagtcacagg
    10381 ggaggagcaa tcctccctta cccttactca ctaaattagg ttaaagaatc aacgatgtca
    10441 atcagggcga tgcaggtgta tcgcccttac cactcccaga ctttcgacgg tgtaaccacc
    10501 gcaggaagag ggatatccca ctcttcaacg gggagttttt ccaccaactg acaatcatgc
    10561 gcataaccca ccggttgcgt tttatagtgc tgccagtttt gtaaggtccg atcataaaaa
    10621 ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg tgtgatcagc
    10681 acgtctaatc gggaaagggg tagcacgtca cgcacatcca gttttggctc atggatcttc
    10741 aacctgttca tcaccagttc gctttgcgga tggtaattca ggaacagcaa attaccggca
    10801 ctaaagggat gcaaaactgg aagatatacg cgcttaccgg cgcgccagag ttgttctatt
    10861 agcggctggg tgtcgagttc accatcaaaa gagaggaata cagcgaccgt atgcgccatc
    10921 accaccgggg gataagtcat catccgggta gcggcttgtt gacccatttc ctgctgttgt
    10981 tccggcgtta acgcacgacg acgttgccga atcattttgc ggatttcttg tcgggataat
    11041 gtcagtggga gttctggtag ttgcgtcatg gctggtgcca gataagaagg gaatctccga
    11101 gatgccgccg caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg tcgtcgcagt
    11161 tttaaggctt ctcggacgga ccgagcatgc tcaccaaccg cggagcgcca cattcttgtg
    11221 gtatgaaata tcggctcagg ggactggccc gcttgcgaac atctcagaga aattttgtct
    11281 tcacggttac tctaccacag taaaccgaaa agtgtcattc aaagttttgg ttagtttttt
    11341 cggtgatgcg accttgttca agtaacgctt gttctatggt ctgctgcagc atccgaatac
    11401 gctgttccat acttgccgcg tagtcacgag tcttcgcttt ttcttgcgct aactcatagc
    11461 tgatattcaa tgcggcaatg aagaccaact gttcagtatt tgtgactcta gtgcgttctt
    11521 tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg attcaacgca tccctttggt
    11581 caggcgggca gttcacacgc agtgaacggc caaaaatttg gatatcgacg ggttgtgcag
    11641 acatgccacc ttcctgctga ttgactgcgc tgccttcgtc tttagaccca tggtctgcga
    11701 aggggcgaca ctatagctac cctgatgaga agagacaagc ccttttctgg tccaccaggg
    11761 cccaaagtgg tagcatatca tgaatattcc tccctttgac gacgaatgct tatgtctata
    11821 cagaacgaaa tgcctggtta caacgaaatg aaccagtatc tgaaccaaca agggacgggt
    11881 ctgacccctg ctgagatgca tggtttaatc agtgggatga tatgtggcgg taacgatgac
    11941 agctcatggc taccgctact tcacgacctg acgaacgaag gcatggcttt cggtcatgag
    12001 ctggcacagg cactgcgtaa aatgcactct ggcaccagcg atgccctgca ggatgacggc
    12061 ttcctttttc agctttatct gcctgatggc gatgatgtca gcgttttcga tcgggctgat
    12121 gcattggcag gttgggtcaa tcacttcctg cttggtcttg gcgttacgca accgaagctg
    12181 gataaagtga ccggcgaaac cggtgaagct atcgacgatc tgcgtaacat tgcgcaactg
    12241 ggttacgacg aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga gatcatcgaa
    12301 tacgttcgtg ttgccgcgct gttatgccac gacaccttta ctcatccgca accgaccgcg
    12361 ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga gtgttatgag
    12421 tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc aaatgcaacc
    12481 cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg acagcgaata
    12541 cccctatcgt cagaacagtg acttctggta cttcaccggc tttaacgaac cggaagcggt
    12601 gctggtgctg attaaaagcg atgacactca taaccacagc gttctgttta accgcgttcg
    12661 cgacctgacg gcggagatct ggtttggccg tcgcttaggc caggatgccg cgccagagaa
    12721 actgggcgtt gaccgcgcac tggcattcag cgaaatcaat cagcaacttt atcaactact
    12781 taacggcctg gatgtggttt accatgccca gggcgaatat gcatatgctg atgaaatcgt
    12841 gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa aatctcaccg caccggcaac
    12901 gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg ttcaaatcgc cagaagagat
    12961 tgccgtactc cgccgcgcgg gagaaatcac cgccctggca catacccggg cgatggaaaa
    13021 atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa attcaccacg aatttaaccg
    13081 ccacggtgcg cgctatcctt cctataacac cattgtcggc agcggtgaaa acggctgcat
    13141 tttgcactac accgaaaacg agtgtgaact gcgtgacggt gacctggtgt tgattgacgc
    13201 gggttgtgaa tacaaaggtt acgctggcga tattacccgc accttcccgg tcaacggcaa
    13261 attcactcag gcccagcgtg aaatctacga cattgtgctg gagtctctcg aaaccagctt
    13321 gcgcctgtat cgtccgggaa cttccatgca ggaagtcacc ggtgaagtgg tgcgcatcat
    13381 ggttagcggc ctggtaaaac tcggcattct gaaaggtgat gttgatgaac tgatcgctca
    13441 gaacgcccat cgtcctttct ttatgcatgg ccttagccac tggttagggc tagatgtcca
    13501 tgacgttggc gtttatggtc aggatcgctc gcgcattctg gaaccgggga tggtactgac
    13561 cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg ccagaacaat atcgcggtat
    13621 cggcattcgt attgaagacg acattgtgat taccgaaacc ggcaacgaaa acctcaccgc
    13681 cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg gcagctgcga gaaagcaatg
    13741 agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc tatttcccgg
    13801 ttaagtcacg gggcgttgcc ggtacatttg attgaagcga ctgcgccaga gtcacatgct
    13861 catccgggct ttgatggacg agcgatagcg ctggcggcgg gtacctgtca gcaactggcg
    13921 cgcatcggcg tttggcaatc tctggcggat tgcgcaactg ccatcaccac cgtgcatgtc
    13981 agcgatcgtg gtcacgctgg atttgtcacc ctcgccgcag aagattacca actggcggcg
    14041 ctgggacagg ttgtcgaatt gcacaatgtc gggcaacggc tgtttgcatt gctgcgtaaa
    14101 gcacctggcg taacgctgca ttgccctgat cgcgtggcta acgttgcccg tactcagagt
    14161 cacgttgaag tgacgctgga gagtggcgag acgctgacgg gccgcgtgct ggtagccgct
    14221 gatggcaccc attcagcgtt agccaccgcg tgcggcgttg actggcagca ggagccttac
    14281 gaacaactgg cagtgattgc caacgttgct acttctgttg cgcacgaagg ccgcgctttt
    14341 gaacgcttca cgcaacatgg cccgctggcg atgttgccga tgtctgacgg acgctgttcg
    14401 ctggtctggt gtcatccact ggaacggcgc gaagagctgc tgtcgtggag tgacgagaag
    14461 ttttgccgtg aactccagtc ggcctttggc tggcgacttg gaaaaattac ccacgccggt
    14521 aaacgcagtg cttatccgct ggcgttaacc cgcgccgcca gagctattac ccatcgtacc
    14581 gtgctggtgg gcaatgcggc gcaaactctg cacccgattg ccgggcaagg gtttaacctc
    14641 ggtatgcgag atgtcatgag ccttgcggaa accctgactc aggcgcatga gcgcggagaa
    14701 gacatcggtg attacggcat attgtgccgc tatcagcagc gtcgacagag cgatcgcgaa
    14761 gcaaccattg gcgtcacgga cagccttgta catctttttg ccaaccgttg ggcaccgctg
    14821 gttgtcgggc gcaacatcgg actgatgacg atggaattat tcaccccggc acgcgatgtg
    14881 ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc ggttttaagg aataaacaat
    14941 gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg gggctggcgg ttgcctgcgg
    15001 cttacagggg agcggcttac gcgttgccgt actggagcag cgcgtaccgg aacctctggc
    15061 ggcggatgca ccaccacaac tgcgcgtttc ggctatcaat gccgccagcg aaaaattact
    15121 cacccgtctt ggcgtctggc aggacattct ctctcgtagg gctagttgtt atcacggtat
    15181 ggaagtgtgg gacaaagaca gttttggtca catttcgttt gacgatcaaa gcatgggcta
    15241 tagccatctt gggcatatcg ttgaaaactc agtgattcac tacgcgctgt ggagcaaagc
    15301 gcagcaggcg tcagatatca ccctgttggc ccccgcagaa ttacagcagg tcgcctgggg
    15361 agaaaatgaa actttcctga cgctgaaaga tggcagcatg ttaacggcgc gtctggtgat
    15421 tggcgcggac ggcgctaatt cctggttgcg caacaaagca gatattccgc tgactttctg
    15481 ggattatcag catcacgcgc tggtagcgac cattcgcacg gaagaaccgc atgatgcggt
    15541 ggcgcggcag gttttccatg gcgaaggcat tctggccttt ttaccgctta gcgatccgca
    15601 tctttgctcg attgtctggt cactgtcgcc agaggaagcg cagcggatgc agcaggcaag
    15661 tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat aatcgcctgg gcttatgcaa
    15721 ggttgagagc gcgcgtcagg tgttcccact gacggggcgt tatgcgcgcc agtttgccgc
    15781 gcaccgtctg gcgctggtgg gcgacgccgc gcataccatt cacccgctgg cggggcaggg
    15841 ggtaaatctt ggctttatgg atgctgcaga gctgattgct gaactgaaac ggttgcatcg
    15901 tcagggtaaa gacatcgggc agtacattta tctgcgtcgc tatgagcgta gccgcaagca
    15961 cagttcggcg ctgatgctgg ctggtatgca gggattccgc gatctgtttt ccggtaccaa
    16021 tccggcgaaa aaactgctgc gtgatattgg tttgaaactg gccgacacgc ttcctggcgt
    16081 taaaccacaa ctcatccgcc aggcaatggg attaaacgat ttgcctgaat ggctgcgtta
    16141 aaaatttctc ttctgttgtt tatttgatac ccatcacact ttcatctccc ggttttttcg
    16201 ccgggagatt ttcctcattt gaaataaact aatttcacct ccgttttcgc attatatttt
    16261 ctaatgccat gattttttga tttagtgttt tttgacattt ttttagttct taatattgcc
    16321 ttattcaaat taactttcta atggcatcat ctttatatag aaactagtgt gttttttggt
    16381 tttttattct gtcgcgattt tggcgttttt taaccataag ctaatgtgat gatcaatttt
    16441 accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg
    16501 gggaccggga gtggctccga tgctgggttt cgtggtgata atttcaccat gaaaagttgt
    16561 cagccccgct tattcaatga ggacaagatg gcacaacaga ctcctttgta cgaacaacac
    16621 acgctttgcg gcgctcgcat ggtggatttc cacggctgga tgatgccgct gcattacggt
    16681 tcgcaaatcg acgaacatca tgcggtacgt accgatgccg gaatgtttga tgtgtcacat
    16741 atgaccatcg tcgatctccg cggcagccgc acccgggagt ttctgcgtta tctgctggcg
    16801 aacgatgtgg cgaagctcac caaaagcggc aaagcccttt actcggggat gttgaatgcc
    16861 tctggcggtg tgatagatga cctcatcgtc tactacttta ctgaagattt cttccgcctc
    16921 gttgttaact ccgccacccg cgaaaaagac ctctcctgga ttacccaaca cgctgaacct
    16981 ttcggcatcg aaattaccgt tcgtgatgac ctttccatga ttgccgtgca agggccgaat
    17041 gcgcaggcaa aagctgccac actgtttaat gacgcccagc gtcaggcggt ggaagggatg
    17101 aaaccgttct ttggcgtgca ggcgggcgat ctgtttattg ccaccactgg ttataccggt
    17161 gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg ccgatttctg gcgtgcgctg
    17221 gtggaagcgg gtgttaagcc atgtggcttg ggcgcgcgtg acacgctgcg tctggaagcg
    17281 ggcatgaatc tttatggtca ggagatggac gaaactattt ctcctttagc cgccaacatg
    17341 ggctggacta tcgcctggga accggcagat cgtgacttta tcggtcgtga agccctggaa
    17401 gtgcagcgtg agcatggtac agaaaaactg gttggtctgg tgatgaccga aaaaggcgtg
    17461 ctgcgtaatg aactgccggt acgctttacc gatgcgcagg gcaaccagca tgaaggcatt
    17521 atcaccagcg gtactttctc cccgacgctg ggttacagca ttgcgctggc gcgcgtgccg
    17581 gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc gtgaaatgcc ggttaaagtg
    17641 acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt gatttacttt tttggagatt
    17701 gattgatgag caacgtacca gcagaactga aatacagcaa agaacacgaa tggctgcgta
    17761 aagaagccga cggcacttac accgttggta ttaccgaaca tgctcaggag ctgttaggcg
    17821 atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt tagcgcgggc gatgactgcg
    17881 cggttgccga atcagtaaaa gcggcgtcag acatttatgc gccagtaagc ggtgaaatcg
    17941 tggcggtaaa cgacgcactg agcgattccc cggaactggt gaacagcgaa ccgtatgcag
    18001 gtggctggat ctttaaaatc aaagccagcg atgaaagcga actggaatca ctgctggatg
    18061 cgaccgcata cgaagcattg ttagaagacg agtaacggct ttattcctct tctgcgggag
    18121 aggatcaggg tgaggaaaat ttatgcctca ccctcactct cttcgtaagg agagaggttc
    18181 acaattcact gcacgtttca ggaaccatcg ctcatgacac agacgttaag ccagcttgaa
    18241 aacagcggcg cttttattga acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg
    18301 ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg gccagattgt gccgaaagat
    18361 attcagcttg cgaccccgcc gcaggttggc gcaccggcga ccgaatacgc cgcactggca
    18421 gaactcaagg ctattgccag tcgcaataaa cgcttcacgt cttacatcgg catgggttac
    18481 accgccgtgc agctaccgcc ggttatcctg cgtaacatgc tggaaaatcc gggctggtat
    18541 accgcgtaca ctccgtatca acctgaagtc tcccagggcc gccttgaagc actgctcaac
    18601 ttccagcagg taacgctgga tttgactgga ctggatatgg cctctgcttc tcttctggac
    18661 gaggccaccg ctgccgccga agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat
    18721 gccaaccgct tcttcgtggc ttccgatgtg catccgcaaa cgctggatgt ggtccgtact
    18781 cgtgccgaaa cctttggttt tgaagtgatt gtcgatgacg cgcaaaaagt gctcgaccat
    18841 caggacgtct tcggcgtgct gttacagcag gtaggcacta ccggtgaaat tcacgactac
    18901 actgcgctta ttagcgaact gaaatcacgc aaaattgtgg tcagcgttgc cgccgatatt
    18961 atggcgctgg tgctgttaac tgcgccgggt aaacagggcg cggatattgt ttttggttcg
    19021 gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac acgcggcatt ctttgcggcg
    19081 aaagatgaat acaaacgctc aatgccgggc cgtattatcg gtgtatcgaa agatgcagct
    19141 ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc aacatatccg ccgtgagaaa
    19201 gcgaactcca acatttgtac ttcccaggta ctgctggcaa acatcgccag cctgtatgcc
    19261 gtttatcacg gcccggttgg cctgaaacgt atcgctaacc gcattcaccg tttgaccgat
    19321 atcctggcgg caggcctgca acaaaaaggt ctgaagctgc gccatgcgca ctatttcgac
    19381 actctgtgtg tggaagtggc cgacaaggcg ggcgtactgg cgcgtgccga agcggctgaa
    19441 atcaacctgc gtagcgatat cctgaacgcg gttgggatca cccttgatga aaccaccacg
    19501 cgtgaaaacg taatgcagct tttcagcgtg ctgctgggcg ataaccacgg gctggagatc
    19561 gacacgctgg acaaagacgt ggctcacgac agccgctcta ttcagcctgc gatgctgcgc
    19621 gacgacgaaa tcctcactca tccggtgttt aatcgctacc acagcgaaac cgaaatgatg
    19681 cgctatatgc actcgctgga gcgtaaagat ctggcgctga atcaggcgat gatcccgctg
    19741 ggttcctgca ccatgaaact gaacgccgcc gccgagatga tcccaatcac ctggccggaa
    19801 tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg aaggttatca gcagatgatt
    19861 gcgcagctgg ctgactggct ggtgaaactg accggttacg acgccgtttg tatgcagccg
    19921 aactccggcg cacagggcga atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc
    19981 cgcaacgaag ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg aactaacccc
    20041 gcttctgcac atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa aaacggcaac
    20101 atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct ctcctgtatc
    20161 atggtgactt atccttctac ccacggcgtg tatgaagaaa cgatccgtga agtgtgtgaa
    20221 gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt
    20281 ggcatcacct cgccgggctt tattggcgcg gacgtttcgc acctcaacct gcataaaact
    20341 ttctgcattc cgcacggcgg tggtggtccg ggtatgggac cgatcggtgt gaaagcgcat
    20401 ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag
    20461 ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc tgccaatcag ctggatgtac
    20521 atccgcatga tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat cctcaacgcc
    20581 aactatattg ccagccgcct gcaggatgcc ttcccggtgc tgtataccgg tcgcgacggt
    20641 cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga aagaagaaac cggcatcagc
    20701 gagctggata ttgccaagcg cctgatcgac tacggtttcc acgcgccaac gatgtcgttc
    20761 ccggtggcgg gtacgctgat ggttgaaccg actgaatctg aaagcaaagt ggaactggat
    20821 cgctttatcg acgcgatgct ggctatccgc gcagaaattg accaggtgaa agccggtgtc
    20881 tggccgctgg aagataaccc gctggtgaac gcgccgcaca ttcagagcga actggtcgcc
    20941 gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc cggcaggcgt ggcagacaaa
    21001 tactggccga cggtgaaacg tctggatgat gtttacggcg accgtaacct gttctgctcc
    21061 tgcgtaccga ttagcgaata ccagtaattc actgattcga ctattttcta aaggcgcttc
    21121 ggcgcctttt tagtcagatg acaaagtaca aaagtgctca gacagtcccc tcgccccttt
    21181 ggggagaggg tgaggggaac aggccggcac tggcgcgaat atttaccctc accccggccc
    21241 tctccctgaa agggcgaggg ggaaaagcgt gccaacattg aagattgagc cagtttgtta
    21301 gcaatctcaa agatacgtca acgaatgaat ttttctcgga aaaacaaatg gctatagcac
    21361 ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgcaagaag
    21421 ggtatacggt ggcggttaat tatcaacaaa acctccacgc ggcgcaggaa gtgatgaact
    21481 taataacgca agccggtggc aaagcattcg tgctccaggc ggatatcagc gacgaaaacc
    21541 aggtcgttgc gatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca
    21601 ataacgccgg gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca
    21661 accgagtact ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac
    21721 gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct
    21781 cacggttggg ttcgccaggg gaatatgttg attacgcggc atcgaaaggg gcgattgata
    21841 cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg gatccgggtt aactgcgtgc
    21901 ggccagggtt tatttatacc gaaatgcacg ccagcggcgg cgagcctgga cgcgtcgatc
    21961 gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca
    22021 ttgtctggct actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg
    22081 gcgggaaata aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt
    22141 catcgactta aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct
    22201 tcttacgtga acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc
    22261 cgtagcgttt gctgtactgc ccggtggtga acgatacgca gtcgatgcag ccccacggtg
    22321 tgtagcccat cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg
    22381 cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt
    22441 aagcgccaaa accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc
    22501 aaagggcata gcgcagacct actggatcaa tctgccagcc ccagtcagac gctttaacat
    22561 acgggttcgg tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct
    22621 tcactgcgtt ggtcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta
    22681 catcgagatc gccgtcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca
    22741 cataggacgg gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg
    22801 actcctgagc gaacatcaca tcgtccgggt tacagcagta gggatagagc ggcaccatcg
    22861 ccagcataca gccgactttc atctccgggt taatgcgacg cgcagctttc accgccaggg
    22921 cgctggcgac aaactggtga tgcagcacct gatacatcgt ctcttccggg ttctcatgct
    22981 cggtataaac cacgccggag cagcagtaac cgaacagcgg tgcacgccag ttacgctggt
    23041 tgttaatttc gttgaaggtc atccagtatt tgactttatg cttatagcgt tcaaacacca
    23101 cttccgcgaa acggacaaag aaatcaacca ctttacggtt ggtccagcta ccgtattgct
    23161 gcaccagatg cagcggcatt tcaaagtggg agagggtgat caccggttcg atgttgtatt
    23221 tcagcagttc atcgaacata tcatcgtaga acttcagccc ttcttcgttt ggctgagctt
    23281 catcaccttt tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca
    23341 tttcggcaaa tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt
    23401 ttggatagta tttgcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc
    23461 caccggtcag aacgtcacaa atgctcggcc ctttgccgcc tttgttccag ccgccttcga
    23521 cctgatgagc ggcaactgcg ccgccccata agaaatcttt cggtaaggtg agttttttca
    23581 ctatcatagg ctccagatga atcaattctt acaggaaata gtaccatgca gccaaacgac
    23641 atcacttttt ttcaacgttt ccaggatgac attctggctg gacgtaaaac catcaccatc
    23701 cgcgacgaat ctgaatcgca ctttaaaacg ggcgatgtgc tgcgcgttgg gcgctttgaa
    23761 gatgatggct atttttgcac catcgaagtc accgcaacct caaccataac gctggagacg
    23821 ctgacggaaa aacatgcaga gcaggaaaat atgaccctgc ctgaactgaa aaaggtcatt
    23881 gccgacatct atcccggcca gacacagttt tatgtgattg aatttaaatg cctttaaatt
    23941 gaactcacgc ctgttagttg aaatttcgca tttaatttca tgatttttcg gagattagtc
    24001 caaaatcaga atttatttta gctaacaggt gttcactgga actattctca gttacgctgg
    24061 agaggtatac atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat
    24121 taagcaggga tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggct
    24181 ggtgtttggt atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag
    24241 cgccacggcg ataaccagtt atagcctata tggcggcagt atgatcctgc tgttcctcgc
    24301 ttcgacgctc tatcacgcca ttccccatca acgggcaaaa atgtggctga agaaatttga
    24361 ccattgcgcc atttacctgt tgattgccgg aacctataca ccgtttttgc tggtggggtt
    24421 agattctccg ttagcgcgcg gattgatgat tgttatctgg agcctggcat tgctggggat
    24481 tctgttcaaa ctgaccatcg cgcaccgatt taaaatttta tctctggtga cttatctggc
    24541 gatgggctgg ttgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcaggcag
    24601 cgttacctta ctggcggtag gcggcgtggt ttattcgctc ggggtgattt tctatgtctg
    24661 caaacgcatt ccgtacaatc atgccatctg gcacggcttt gtactcggcg gtagtgtgtg
    24721 ccactttctg gcgatctatt tgtatattgg gcaggcgtaa ttgctgtgcc tttgtctgca
    24781 tgaaaagaca catcagtgtc gcttcaggca atagatgccc gaagcgatat agaaatttac
    24841 tcttcgagcg aatacggcag cggctcgata tgcagtgtat ttgcatcgtc gcgcacgcgg
    24901 aagatgctgt ccggttccat atcgttattc atcaccacct gcaccacgac ctgaccatct
    24961 tccagtttga ccgcagccag caccgtaccg gtacgacgcc agttttcgcc cattttcagc
    25021 tctaagtctt cacctgcttc tggcagtcgg ctggcgctac ctgccagtaa ccagagcgca
    25081 cgtttgttgg caccgcggaa tttggcccgc gccaccatct cttgtccggt ataacagcct
    25141 ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg
    25201 ctgttggcgg catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg
    25261 ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca
    25321 tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct
    25381 tctttgacta cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg
    25441 cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact
    25501 ttagagaata ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt
    25561 tcaatccatg caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct
    25621 ttggcgtcgc aatgggcggc gagcaggtgc tgatcttctg ccatctggct gacatctgct
    25681 gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag
    25741 tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acggggagga
    25801 aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg
    25861 taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc
    25921 gacttccaaa aaaatgaagc cattgaattg ttgtgaatta atttcgcgat cgcgatggtg
    25981 tttactgata ttcccgtaaa agaaggcgaa aaacacgtta caatagccgg gtactggatt
    26041 ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg
    26101 ccgtggtatg cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag
    26161 cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct
    26221 gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg
    26281 actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct
    26341 ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca
    26401 tgccctggcc actcagttac accccgttat ggatggtgtt actttcgctg gtggtgtttg
    26461 attgcgttcg cagccagcgg cgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg
    26521 acgggcgttt gcgttggcaa gggcaggagt ggtgcatcgt caaagcaccg tggatgatta
    26581 agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg
    26641 cagccgacag catggacgaa gccgaatggc gggatttacg gcggattttg ttgcaacaag
    26701 agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg
    26761 taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta
    26821 aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc
    26881 accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg
    26941 cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat
    27001 cccagttgat cgccaagccc atacagcgca acaattttac cttcaaggtt caggtcgtcg
    27061 agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata
    27121 cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt
    27181 aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa
    27241 caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt
    27301 ggcttttgcg gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat
    27361 caaatgagtg accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc
    27421 ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg
    27481 atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa
    27541 gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct
    27601 cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc
    27661 gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag
    27721 atttaagcga agcgcaggtc gaacgtttat tacaggcacc attaattgat cagccactgg
    27781 agctacgcga taaagccatg cttgaagtgt tgtatgctac cggactgcgt gtctctgaac
    27841 tggtcggact gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta
    27901 aaggcaacaa agagcgcctg gtaccgttag gtgaagaggc ggtttactgg ctggaaacct
    27961 atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca
    28021 gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgctg
    28081 tgctggcggg tatcgacagc gaaaagctgt caccgcatgt attgcgtcac gcttttgcca
    28141 cccatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg
    28201 acctctccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc
    28261 aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt
    28321 ttatgttgtt tactttgtta gcggcgtttt caggttttgc tcaggctgat gacgcggcaa
    28381 ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag
    28441 ctggcatgaa gacagttctg actaacagcg gcgtgttgta catcaccgat gatggtaaac
    28501 atatcattca ggggccaatg tatgacgtta gcggcacggc tccggtcaat gtcaccaata
    28561 agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc
    28621 aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc
    28681 atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc
    28741 gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata
    28801 aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg
    28861 tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag
    28921 ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat
    28981 tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca
    29041 acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct
    29101 gcgccgttta tacgccagcc ggggcgtgcg cagtgcgcaa gaactggaac gcagtgttaa
    29161 aggtatgttg ccctggcagc aactgagcgg cgtcgaaaag gccgttgaga tcctttacaa
    29221 cgcttttcgc gaaggaacgc ggattattgt ggtcggcgat tttgacgccg acggcgcgac
    29281 cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct
    29341 ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca
    29401 tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggggt
    29461 tgaacacgct cgctcgttgg gcattccggt tattgttacc gatcaccatt tgccaggcga
    29521 aacattaccc gcggcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc
    29581 gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt
    29641 gcgcgatcag ggctggtttg atgagcgtgg catcgcaatt cctaacctgg cagaactgct
    29701 ggatctggta gcgctgggga cagtagcgga cgtcgtgccg ctggacgcta ataatcgcat
    29761 tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccgg ggattaaagc
    29821 gctgctggaa gtggcaaatc gcgatgcgca aaaactcgcc gccagcgatt taggttttgc
    29881 gctggggcca cgtctcaatg ctgccgggcg actggacgat atgtccgtcg gtgtggcgct
    29941 gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa
    30001 ccagacgcga aaagagatcg aacaaggaat gcaagttgaa gccctgaccc tgtgcgagaa
    30061 actggagcga agtcgcgaca cgctacccgg cgggctggca atgtaccacc ccgaatggca
    30121 tcagggcgtt gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat
    30181 cgcctttgcg ccagcaggtg atggtacgct gaaaggttca ggtcgctcca ttcaggggct
    30241 gcatatgcgt gatgcactgg agcgattaga cacactctac cctggcatga tgctgaagtt
    30301 tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca
    30361 acaacggttt ggcgaactgg ttacagagtg gctggaccct tcgctattgc aaggcgaagt
    30421 ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agttgctgcg
    30481 cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct
    30541 gctgcaacag cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg
    30601 cggtccactg ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg
    30661 cgtgcgtgaa gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag
    30721 cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga
    30781 gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa
    30841 tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc
    30901 cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa
    30961 acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta
    31021 aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg
    31081 aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg
    31141 aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta
    31201 tgttctctgg cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg
    31261 gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggctgaat
    31321 cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat
    31381 ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg
    31441 ttcaccgcct ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acatcgttca
    31501 gctctgcgtt tgtttacccg gaagttgatg atgatattga tatcgaaatc aacccggcgg
    31561 atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg
    31621 aatctgcggt gcgtattacc cacatcccaa ccgggatcgt gacccaatgc cagaacgacc
    31681 gttcccagca caagaacaaa gaccaggcca tgaagcagat gaaagcgaag ctttatgaac
    31741 tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa tccgacatcg
    31801 gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgta
    31861 ccggggtaga aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg
    31921 aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg
    31981 ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc
    32041 tgcgcgagca ggggattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat
    32101 tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg
    32161 ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc gttaccctgc
    32221 aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg gaaggcgttt
    32281 acaacgagca gttcaaaaaa tgggacctcg gtgacatcct cggcgcgaaa ggtaaactgt
    32341 tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg ctgaccaaag
    32401 cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc
    32461 agcgttatct ggatctcatc tctaacgatg aatcccgtaa cacctttaaa gtgcgctcgc
    32521 agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc
    32581 cgatgatgca ggtgatccct ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg
    32641 cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg
    32701 tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatctccg
    32761 tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag
    32821 atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga
    32881 cggaagtgac ctatggcgac gtgacgctgg atttcggtaa accgttcgaa aaactgacca
    32941 tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gataacttcg
    33001 actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc tggggtctgg
    33061 gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct
    33121 tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg
    33181 aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg
    33241 agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg
    33301 caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg gaacatggct
    33361 taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg ttcaccaaca
    33421 gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa taagcattac
    33481 gttatgctca caaccccggc aaatgtcggg gttttttatt taagctgggt aaatgcagat
    33541 aatcgtattc tggcttcgcg atttgtcgcc tgcatcacca tccacggact aaacgcccac
    33601 ggcgtggcat caataccgtg taatacatca gctaaatcac accattgata atccatcact
    33661 tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca
    33721 ttttccacaa tgccactcgg atcggtggcg cggtagcgaa agtcaggata gatagattca
    33781 ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcaccgc gtcttcgctg
    33841 ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccacgctttt
    33901 ttgctcagtg cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag
    33961 aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtaccagtg
    34021 ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa
    34081 ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa gtgagcgttt
    34141 atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca
    34201 tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag
    34261 agagagtaaa aagcaacagc agactgaata tagcgcgcat taaaaaattc atattattct
    34321 ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgactttt
    34381 aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctggta gccattaaag
    34441 aagacattca gcacaacggc gctaagggtt gcgagcataa tgccgctatg cagcaacggt
    34501 tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg
    34561 ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac
    34621 agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ccagcccagc accgcccagc
    34681 acaaattgcg gaatggaggc gaccagcacc gccatttttg gcaccatgcc aaacaggatt
    34741 aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcgtcac gctaaccagg
    34801 ccaacgtttt gtgaaaatga cgtgtggggg aagctgttaa acgttccgcc tatcattgtc
    34861 cctacgccat cgacacgcag cccgcgaata atatcgtgtg aagagagttt acgaccgact
    34921 atttcaccca gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca
    34981 gtcatggtca gaatggaaac gggatcgaaa atcggcatac caaatgacat cggcgtgaca
    35041 atcgcaaacc atgaagcatc atgtagcccg gataaattga cttcattcat catccacgaa
    35101 agtaaaaagc caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca
    35161 tagcgagtaa tgagcaagat aaaaattaag acagcaaagg agatacctaa ataaacggga
    35221 ttaccatatt gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg
    35281 ctaagcccaa tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag
    35341 cgaccgataa gtggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat
    35401 atccccagca ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca
    35461 aaggtcaccg acataatcac cggcaggcgg atccccataa agcggccgat accgatacat
    35521 tgcaataatg tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagct
    35581 tcttttgaga ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac
    35641 atgacaagga cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca
    35701 acctcatcag ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc
    35761 atcagtgttt ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg
    35821 tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat
    35881 tgcggaggca atcacataat cgtggttaaa ccacgaaaat gagaagagaa cctgacaatg
    35941 aaattatctg taattttctc atattgatat ttcattaatc attctggcat gtcgcactct
    36001 cgcatttaat cgtttttatc tggatagcgc tcttttggtc ggcaatgttg aaataatgaa
    36061 agcggcatag attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt
    36121 catgctacct aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt
    36181 aaatgaaagg gatgcaaatg aataagttta tcgctgctga agctgcggaa tgtataggct
    36241 gtcatgcttg tgaaattgcc tgtgcggtgg cacacaatca agaaaactgg ccgctgagtc
    36301 acagtgactt tagaccgcgt atccacgttg tagggaaagg ccaggctgcg aatccggtgg
    36361 cctgccatca ctgcaacaat gccccttgcg ttacggcttg tccggttaat gctctgactt
    36421 tccagtccga tagcgtacaa ctggacgagc aaaaatgtat tggttgtaaa agatgcgcaa
    36481 tcgcttgccc ctttggcgtc gttgagatgg tcgatacgat tgcgcagaaa tgcgaccttt
    36541 gtaaccagcg cagttctggc acgcaagcct gtattgaagt ctgcccaaca caggcgttac
    36601 gactgatgga cgataaaggg ttacagcaga taaaggtggc ccgccagcgc aaaacggcag
    36661 caggaaaagc gtcatcagtc gctcagccat ctcgcagtgc agcgttgctc cccgttaact
    36721 cgcgtaaagg cgcagataaa atttcagcga gtgaacggaa aacccacttt ggcgaaatct
    36781 attgcgggct ggatccactg caagcgactt atgagagtga ccgctgtgtt tattgtgccg
    36841 aaaaagctaa ctgcaactgg cattgcccgc tgcataacgc tattccggat tacatccgtc
    36901 tggtacagga aggaaagatt attgaagcgg cagaactttg ccaccagacc agttccttac
    36961 cagaaatctg cggcagggta tgcccacagg accgtctttg tgaaggcgca tgtactttga
    37021 aagatcactc tggcgcagtc tctatcggta atctggaacg ctacatcacc gataccgcgc
    37081 tggcgatggg ctggcgtccc gatgtcagca aagttgttcc ccgtagcgaa aaagtggcgg
    37141 tgattggcgc tgggccagca gggttagggt gtgctgatat tctggcgcgc gcgggagttc
    37201 aggttgatgt ctttgatcgc catccagaaa ttggcggtat gctgactttt ggcattcctc
    37261 ctttcaaact cgataaaacg gtattaagcc agcggcgaga gatattcacc gcaatgggaa
    37321 tcgactttca tcttaactgt gaaattggcc gcgatattac ctttagcgat ttaacttctg
    37381 aatatgatgc agttttcatc ggcgtgggga cttacgggat gatgcgagca gatctgccgc
    37441 atgaagatgc acccggtgtc attcaggctc taccgttcct gactgcccat acccgccagc
    37501 tcatgggatt gccggagtct gaagagtatc cgctgacgga cgtggaaggt aagcgagtcg
    37561 tggtattggg cggtggcgat acgacaatgg attgtttgcg gacttccatt cgcctcaatg
    37621 ctgccagcgt gacctgcgcg tatcgtcgtg atgaagtcag tatgccgggc tcgcgcaaag
    37681 aggtggttaa tgcgtgcgag gaaggtgtcg agtttcagtt caatgttcaa ccgcaatata
    37741 tcgcttgtga cgaagatggg cgtttaactg cggtgggcct gattcgtacc gccatgggtg
    37801 agccggggcc ggatggtcgc cgtcgtcctc gtccggtagc gggttcagag tttgaattgc
    37861 ccgccgatgt tctgattatg gcctttggtt tccaggcgca tgccatgccg tggttgcagg
    37921 gcagcggaat taaactcgat aaatggggcc tgattcaaac cggtgacgtc gggtatttac
    37981 ctacccagac gcatctgaaa aaagtctttg ctggtgggga tgcagttcat ggcgcggatc
    38041 tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg cgatatgtta actctgtttg
    38101 atacgaaggc atcgtgatga aatcgttaat tatcgttaat ccggctgact gtattggctg
    38161 ccgtacttgt gaagtagcct gtgtggtcgc tcacccttca gaacaggagt tgaatgccga
    38221 tgtcttttta ccccggctga aggtacagcg actggacagc atcagtgcgc cggtgatgtg
    38281 ccatcagtgt gaaaacgccc cttgtgttgg cgcttgcccc gtgggggcgc tgacgatggg
    38341 cgagcaggtg gtgcagacca attctgcccg ttgtattggc tgtcagagtt gcgtcagtgc
    38401 gtgcccgttt ggaatgatca ccattcagtc gttgccgggg gatacccgac aacaaattgt
    38461 gaaatgcgat ctctgtgaac agcgggaaga ggggcctgcc tgcgttgaat cttgcccaac
    38521 acaggcgctg cagttgctta ctgaaagaga actcaggcga gtccgccagc agcgtattgt
    38581 tgccagcggt gagaatcctc tgtgaaagaa atctccgcgc tgaaaggcgc ggagaatcta
    38641 ttagattgcc caaccacccg cgtaaaatgc gaccagtgcg gcagtaatga tcaccgtacc
    38701 aatattcagc ttttgccatt cgcgtgcaaa gacgcggcct acgaccagtg tcacaaagcc
    38761 cagcataata ccggtaacga tattacaagt cagaacgatg aacacggcac acaccaggcc
    38821 agccatagcg tcaataaaat cattgaaatc cagcttcgag acgttactta acatcagcaa
    38881 acctacgtac atcagagcgg gtgcagtggc gtaaccaggg atcaaaaatg acagcggtga
    38941 taagaacaga atcagcagga ataacgcccc cactacggtt gcggttaacc ctgttttacc
    39001 cccggcggcg gttcctgccg ctgattcgat ataaaccgct gcgggcgctg cgcccaccag
    39061 gccggagaat attgaactta ctgagtcact ggtcagggct ttgccgccgt tgatgatctg
    39121 gttgtcttta tccaacaaat tcgcctgacc ggcgacggca cggatggtgc cagtagcgtc
    39181 gaacactgcg gtcatcacca atgccagtac actcggaagt acagttggct ggagtgcgcc
    39241 cataatatcg aggctgaaaa tcagagactt accatcttcg ccagtcaggc ttggcatcgc
    39301 caccagaccg tggtatttca ccgctgggtc aaagattaag ccgatgatcg aaattgcaat
    39361 aatcaccaac aagatcccgc cgggtacgcg acacttctcc agaccgaaga tgaccgccag
    39421 ccccagcaag ctcatcatca ccgggaagga ggtaaacgca ccgagcgcca ctggcaagcc
    39481 ttcaatcggg tttttgataa ccatacccac accgttagca gcaatcagca gcagaaacag
    39541 cccgataccg atacctgtac cgtgagcgat acccatcggt aaattacgta agatccaggt
    39601 acgcacaccg gttacggaaa tagcggtgaa gatgacgccc atcagaaaga ccgcgcccag
    39661 tgcgacagga acgctaattt gttgcccgag taccagactg aatgcggtaa atgccgtcaa
    39721 ggaaatcgca caaccaatcg ccattggcaa attggcccac aaccccatca gcaacgagcc
    39781 gaagcccgcg accagacagg tggcaacaaa cacagctgcg ggaggaaaac ctgctttgcc
    39841 cagcattccc ggaacgacga taacggaata aaccatggcc agaaaggtcg ttaagccagc
    39901 cagtacttcc tgacgaacgg tactgccacg agcggtaatt ttaaaataat tatccagcgc
    39961 gccctgtggc tttggtgcgt ccggtgtttg taggatgtct ccagacataa tgatgtcctc
    40021 acgaggatgt taaagtttta ttattagttg cgttcgtaca ccagacgacc atcaacgtag
    40081 gtgcggtaga tcgaacggtc atcgcccaac gtcatcatca cgaacaattt gtcgactaaa
    40141 gaaacagagt tgtcatagcg cagctgctgt agcggagtgg cggtgggttc catcaccacg
    40201 aaatcagcct ctttgccagg taaaaagttg ccaatcaaat cgtcaaggcc cagagatttc
    40261 gctccgccga gcgtggccag gtaaaacgct tcatatgccg agaggcgata gccttgtaat
    40321 tgcaatactt tgtaggcttc gttcagcgtt tgcagcatgt tgaaagtggt tccggcaccg
    40381 atatccgttc ccatgcccac tttaactttc ttctgccatg cttttttcaa gttgaataag
    40441 ccgctgccga ggtaaaggtt ggaggtcgga cagaaagcaa tgctggattt ggtttcgctg
    40501 agacgatccc actctttttc ttcgagatgg acgcagtgag caaagacaca gtttttaccg
    40561 gtcaggccgt actgatggta aacatccaga taaccatcat ggtcaggata aagcgatttc
    40621 acccaggcaa tttcatcttt gttttcacag agatgggtat gtacccacgt atccggatat
    40681 tcttctttca ggcgttgcgc catcgccatc tgttcaggag atgaggtcgg ggcgaagcgt
    40741 ggcgtaatcg catatagcag acgaccattt ttgtgccagc gttcaatcag ttctttgctt
    40801 tggtgatagc tgctttcggc agtgtcgagc agataatccg gtgcgttgcg gtccatcatc
    40861 accttaccgg caatcatacg catattgata tgactggcgg cttcaaacag cgcatcaaca
    40921 gattgcggat gaacagtgcc aaacaccagc gccgtggtgg ttccgttacg taaaagctgc
    40981 ttgatgaaga atgccgacat ttcgcgggcg tactctaaat cctcataacg acgttcagta
    41041 gggaaggtgt gtttattcaa ccactccagc aattgttcac cataggcccc caccatttca
    41101 ctttgcggat aatggatatg tgtatcgaca aagcccggta ctatcagttt gccgcgatag
    41161 tcgcgcacgc gaatagtgtc aggaatttga tgctttccgt cttcccattc gccaaaccat
    41221 tccacttttc cctgtttaat gagtaataaa ccatcctcaa taaaccgcag cgcagaggca
    41281 atctcttccg ggttatcgac cgtacgggtg acatcaataa aactgcctcg taccgctttc
    41341 aacgtgtgtt ctcctgacat cattgactcc tttactgtta atccatctct tccgctgagg
    41401 taataccagg cagaacgttt tcctgtcggt agccaccagg gaggataata tttaataaaa
    41461 tcgcagttaa cccgccagca caaatagggt tttcaactaa tacataaata gaggctggca
    41521 atattttaaa aatttcggga tcgtaggaga cgccaagccc taaacctaaa gaagtggcga
    41581 caataagtgt ttcacggcgc tttaaaccgt tggtgatgat gatgcgaatc cctgcgatgg
    41641 caatcatgga aaacatcaac gtcattgcgc ctcccagaac tgccgaggga atggtcgtga
    41701 agaagccgcc aatcatcgga aataagccga ggataaccag cattaccgcg atggttcgcc
    41761 cgacataacg tgaagcgacg ccagtcatct gaataacccc attattttgc gcaaacgtgg
    41821 ttaatggtaa tgaaccgaca gcggaggcga taacagaaac cagaccatct gccagcacgc
    41881 cgcctttcag tcgggactga tactcttccc cctgaatggg gcggcgggaa accattgccg
    41941 tggcggtgat atcgccgact gcttccagca cgctaagcag ataaatcgtg ccaaccacca
    42001 ggaactgatg gaagctaaaa ctaaagccgt atttgaacgg atgcgggatg gtgattaacg
    42061 gcaaattgcg catactgctg aaatccacca tgcccaggca taacgatgcg atatagccga
    42121 cacatagccc aatggcgatc cctcccatgc gtagcaacgg actgcgacag cagttaaagc
    42181 cgatcaccac aattaaaacc aataaaccaa cgccgagatg ttcgtaattg ccgaatgtac
    42241 cgctgctttt ggctgcaaat cctccaccaa aatcgataat gccgacttta atcaggctta
    42301 agccgatcat cagtaccaca ataccgctga cggtaggcgt aataacccga cgtaaatagg
    42361 gcaagataaa tgaagatccg acaaccagaa atgcgccaac gaaggagacg ccgagaagcg
    42421 acgacatgat taactcttcg tgaaaaccgt cgcttttcat gctgctgccc agcgcaatca
    42481 tgaccgtaac aaatgaaaaa ttgactgact gaattgagag taggccagaa ccgacgatgc
    42541 cgtagcggtt tacttgtaac caggtaccaa taccagaggc gatcatcgcc atagaaacaa
    42601 gataggcagt tgtttcagcg gaaagctgta aggccgcccc cacgattaac gcgggggtta
    42661 ccatcggaac gaaaattgcc aacagatggg taatggcacc aacgagagcc tgatgaaagg
    42721 gagggcgatc ctccagttca aatataaggt cagaacctgc attgtttata tcagacatcc
    42781 cttcactcct tttaaagtaa atttgatcat ctgaattagt aaaataaatt cccacgaatt
    42841 tggggcgcag gacgccttga gaacggctta tctcattttc gcagtcacta tttagtcatc
    42901 gtccaaacag agaaacaggc cgccgaagcg gccatttatt atttatattt tttccagcgc
    42961 agtgagtatt ttctccggtg tgaaatgcca ttcgcgtaac cagatgccgc atgcatcgtg
    43021 aattgcggta gcaatcgccg gagctgcgcc atttacaccg atttccgaga tcgatttcgc
    43081 cccgaacggg ccgactttat cgtcgctcgg caccagcaca gcgcggaaat cacgcggaat
    43141 gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc gttaacgggt gaccttcggc
    43201 gtcataaatg atctcttcgc tcatactgtg gccgatagct cgcagggtag caccgtagat
    43261 ttgtcccaat gctaattccg ggttgaccgg tgtaccgcag tccagcaagg cgtagaattt
    43321 atccaggcgg atttcacccg tacgcgtgtt gacggcaact tcagcgaagt ttgcgccata
    43381 cgggaaggcg aaatcaggcg tgatataact gccagttccc accagtgaac caaagccggt
    43441 gccggtttcg cctttatgag caatatcccc gaagctgact tcgcctttct tgccgcgcac
    43501 gacgcccggc gttgccagtt gaacatctgc cactgcctca cccaacattt gcgcgccgtg
    43561 gaacagaatt ttctcccgca gattttccgc tgccaaacgc gccgcgttac ccgagaagca
    43621 agtaccggac gaggcatatg cgcctttatc aaacaacgca tgatcggtat caccggagat
    43681 aacatgcacg tcctgcggtg ggcagtgcag cacttctgct gccagtttcg tcactacagt
    43741 atccagacca gtaccaatat ccgcaccgcc agaatgaacg ataaaggtgc cgtccgattc
    43801 cagtttgatc atgcagttag cctgatcgat atccgggata cccgatttct gcatgataat
    43861 ggcgacgccg cgaccgatgt gccagtcact attttgcggt ttcggtgaag accattggat
    43921 catctcgcga ccctgacgca ggatctcttc cagtgcgcag ctggcggcgg aaggaacgga
    43981 ggtcggcgct ttaccttcac cgattgcacc gagaattttc agctcttgcc cttcgtgtac
    44041 ccggttacgt tcgataattt ccagttggtc gatctgtaac tgttcagcca gttccgctaa
    44101 tgccatggtg atagcgaagt tacctttcgg tgcgccataa ccctgataag caccatttgg
    44161 gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg ttatcgcacg gatataacgg
    44221 cagcgacagc gccggtccgt tacacggtac ggtgagtgag tggttgccgt aagggccagt
    44281 gttggcgcgg aaatccatct tcactgccgt caggcgacca tcttttttcg ctcccagttt
    44341 gacggtgact ttcgcgacgt gacgagaggt gttagcaata aactcttctt cacgggtgta
    44401 gcggaacagt accggacgcc cggtcacgca ggttgcccag gcgcacactt cttccagcag
    44461 gatgtcctgt ttggaaccaa aaccgccgcc aactcgctct ttaatgacat gaactttatg
    44521 ctgtttcatg cccacgaggc gcgcgacctg gcggcgtaag tgccatggta cctgggtgga
    44581 ggcgtggata accagacgat cgccgtccat acgagtaaag cagatatgtg tttcagtcgg
    44641 gcactgctgc gcttgcgttg agttataggt tcgctcaatg atcacatcgg catcggcaaa
    44701 gcctttgtcc atatcgccaa tatgaccatg aatactggcg gcgatatttt tgcgagggcg
    44761 agaaccgatc gggaagttga tgatcatatg ctcgccgcgc tgggctgcat ggctgttatc
    44821 gtcttccaga gtatctggcg caccagcaac atacaccacc ggttcatcgt gcacgacagg
    44881 cgcatcttcc gccattgctt cgtcgatcga cattaccggc ttaagcactt catattcaac
    44941 gtcgatgagc ttcagtgctt cgagcgcaat ttcttcactt tctgcgacga ccgcagcaac
    45001 acgatcgccg acgtgacgca ttttcttgcc gaacatacgg cggtcaagcg gtgacggttc
    45061 cggtgcgctc tgaccacccg gggtatagta gatatccggg caattcaggt gagtaataac
    45121 gtgaacgacg cccggtaagg cttcagcttt gctgacatcc agatgagtaa tcagtgcgtg
    45181 agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca gccgttacgc ggtcttcaac
    45241 atagcagggt ttcgcctgca ccattttcgc ggcatcagtt ttaggataat gcttaccaat
    45301 gacttctagg tcgtcacgga aagtcggagc gatatcaatg gtggcctgcg gattattttt
    45361 acgtgcaacc gccagttcaa tgacctgata gtattgctgc cagccagcat cgcggctgaa
    45421 caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt gcggcgatgc gatcgagaag
    45481 atcggtgata atcagagctg cagctggatc gttataacca gactgcacca cgccaacatc
    45541 aaccattgcc tgttgaacca gacttaactc gttccatttg cccagagatt ctgcggtacg
    45601 aatatctgcc ttctctaact gtgcggcaat aagcaaggac gcgttaacga tattaccgtt
    45661 aaagattatt gcgtcagaac cggcaaaccc gaaaccatca tcactgttgc gtacagagtg
    45721 cattcccatg ttaaacaaca gcttttgcac gttttcgcct ggattaacgg ttagctcctg
    45781 aggcgcgcca tttaaagtaa agtggatgat catacggctt cctccccagc ctgttggcag
    45841 tcggcataca ggtcggctac cagaactccc gtgatatagc gtttataggc cacgctgccc
    45901 cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct gttcaagtgc attgccttcc
    45961 agattttgtt tttcgacatc atgcagacgc agtgctttac tggcgacgcc atccagcgca
    46021 attcgcatac cgtcgtggtc tgtcattgca acggctgccg tcacgacggt taaacctgcc
    46081 tgagaacggc taattttgcg ggtcgcacag gtgcgatacg gatctttaat gataatttcg
    46141 gttagcaggc gatcgcatgg gcaggccagg tagtcctcga ttgacagcgt ttcgccgttg
    46201 ccaaaaacca gttcagcatc cagtgccaac aggacgggaa gcagcaccga ctcttcctgg
    46261 cgggcggcga tttcaccacc aatggtcgac tgattacgaa catggcgtga gtaaacaaaa
    46321 ccgagggctt cacgcagcgc tgcaggaata aatcgcgcat cacgcagtgg ctgcaagcga
    46381 gacattgcgc caatccgcag tgcaccgtta tcccagtcaa cccagtccag ttccagatcc
    46441 tgtaaggaaa tggcaatctt tttatcggta cgggttggtg tagcattgag tttgctgccc
    46501 ccggcgaacc agacggcttc atcctggtag cggcgcttca gttccagcgc ctgttcgaca
    46561 gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt atttaaccct cttccctgcg
    46621 ggaagagggc atctgtcatt tatgccagcg catccatccg acgccacata ctggcagcgg
    46681 cttttctggc ttgtgcataa atggaatcgc aatcgaagtt aaactgacgg tcttcataga
    46741 ccatcacacc attgaccatt acgctgtgaa cgctgcctga gcccataccg aaagcgatat
    46801 gcccggcgat attgtctgcc agcagcggcg tcggcgagtt gtaatcgcaa atggttaaat
    46861 cagctttgta accggcttcc agaagcccaa atttcgcgcc aaagttgcgg ctcatcagtt
    46921 cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca cagcggacca cccgcatcgc
    46981 gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc cgaaccaatg ccgtccgttc
    47041 ccaacgccag attgcggatg tcgctaaggt gatggttgta gccgacatgg ttgttcatgt
    47101 ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt gagtagggtg atgtcatctt
    47161 tcgacaagta cagcccatga gcgaccagcg ttttgctgtc gatgagatcg aattgcgcca
    47221 gtcgtgccag caggtctttg ccgtaccagt ggtgactgta ggaaacgtcg taaaggtctt
    47281 ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac ggcttcacgc agcatctcca
    47341 gaccggcatc cggcacggta aacggcgcgt gagcaccgat atgtgcttcc accagatacg
    47401 gctcggttgc tgctttcttc gcctcatcaa tctgacgggc aaagcggatg ttttcttcta
    47461 caccttcctg caactctttg atgccgttgt tacggtcagt agtttcaaaa caggtcattg
    47521 cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga gagcgacccg ccgatatacg
    47581 ccggagaggc atggtgatcg ataaccgatg tacatccgct cttaatcgct tccagggaac
    47641 aaatcagtcc gctgtaatag agcgactctt catcaagggc gcgatcgagc cgccaccaga
    47701 gatttttcag cgttgagatg aaatccgggc agggggcgat gtttgccata attccgcggg
    47761 aaagccccga gtaaaaatgg ttgtgcgagc agacaattcc cggcatcaca atccgaccat
    47821 gcatctcttt gtagctggca tcggggtagc gttgcgtcag ggcatcgccg atagcgacaa
    47881 tcacgtcatt ttcgatagcg atatcaacgc cttcctgcac tttcgccggg tgtaactgca
    47941 ctgcggtgac attcttcaga atcaacatga ttacacctcc acgcggccca gcagataatg
    48001 atgatgctgg tggacatggc tgatgatgcg gcacatatcg ttcagctccg gtggtacgtt
    48061 gttaaactga ccttcgctgt cgatgtttaa cacccagctt tggttattca gacgtactcg
    48121 tacccggcaa tcttccacaa ggaagcctgg gttgctgctg ttatcaaagt cttgcgccag
    48181 gctgaagacg gtgattttgt ctttgtatgg tttaccgttc cacggacaga actgagcgca
    48241 gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc tggaatctat tctggaagcc
    48301 agggactgca atggagacgt tggcgcggtt cgggcagaca tccacgcact tgctgcaaac
    48361 gtagttacat tcaaggcagc gagcagcttc ctgcgcgaca aacgcatcac gatcgtcact
    48421 gttcaccagc gtgatagaga tatcgccttt acgttgatag atttccgccg gattgacgtt
    48481 gttccagtat ttattgttct ggtgggaacg gatattttcc cgactaagga tggcatcggt
    48541 cgcccgacgc gcggttccga cagcagcgac aatggaggat ggtccgcgct gtacgtcgcc
    48601 gatcataaag acgtcagtca gacgagtttc gccattatgg tcgacgtctg gccagccgtt
    48661 tttgtccagc ggtacgccca tcgcattcag ggcttcagta tcctgctgtt caccgatggc
    48721 ggtgatcaga ctgtctacat gcagtgttac tgtttcgttg gtttcaaccg gacggcgacg
    48781 acctttctca tcgggttcgc caagcgacat aacgcgcaag gttaaggtgc catcagcatc
    48841 gaaacattcc ggattattca ggaaacggaa ctccacgccg tcgtgcaacg cttcttcata
    48901 ctcttcgcgc catgcgggca tctcttgcag tgaacgacgg taaacgatcg ttgctttttc
    48961 tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt gcggtgttac ccgccccgac
    49021 aacgaccaca tgtttgccca gcttgagcgc tgtacccttg ttgtattcac gcaggaaggg
    49081 gagtgatttc cagacatttt ggttgtcgcc cgccagtttc acaccgctat ttttatcagt
    49141 gccggtggca atcagaacat agtggaagcc ctgatttttt aactgttcaa cggtcaaatc
    49201 gggtgagcag ccatactcaa atttcacgcc gtgagcggca acaaaatcga tatcgtgctg
    49261 aattaactct gcaggaatac ggaactgagg aatgatattt ttcaccacgc cgccagcatt
    49321 ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg gcaaggaagt aacctgctgc
    49381 cagacccgcc ggacctgcac caatcacggc aaccggatgg cgtgaaccag aaccgacggg
    49441 tttgtgccag cgttgcttat attcatccca acctttttcc agcgcgactt ttttcagttc
    49501 gcggatattc agcgcactgt cgtaatccag gcgggtacag ttgtattggc actggtgatc
    49561 gcaaatatga ccggtaatgg cgggcagagc gttgcgttgg tagatgagtt ccagcgcgtc
    49621 ggcatagcgg tgttcgccaa gcagacggat gtattccgga atatcttgct tgatagcgca
    49681 ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc aggtcttctg ccacttcaat
    49741 acgctcttct ggcttccagt gtttctgggt gtattccatg gttaacgcat ctgctgccag
    49801 tctgttcagt cgttcgacgt caacatggtc aagtccccag gcgtcggagc cttccagctc
    49861 gcgcatgcag gcacttaagc gcaaatagcc gccaggtttc agcaggtcgg ttgccatagt
    49921 aatagggcga atacctgtat caaaaatatc gcggatagtc agctgactgg caccaccgga
    49981 ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga actgctgcaa cattgatgga
    50041 gagcgggaac agcgcacggc ctgacatata catctcttca ccaggcagtg cgcctttatt
    50101 gttgatggtg ccgagagtgt tagtcagttt tacgccaaag ccgagtgatt tttcttttgc
    50161 cagtgccatc aggcgttcca gcatttccag tgcttgcgtc agcttgaggt cgtgatcaaa
    50221 tgactcttct tttaagccta tgtaaccgaa accgcagaca tcgaggatct cacgaacacg
    50281 cgcgtatccc agtaaggtcg ggttaagttt cacaaaggtg ttgagccctt tttcttccag
    50341 catgtagcgg caaatggctt cgatttcatg cggaggacag ccgtgcatgg tggagagggt
    50401 gacgccctgc accatactgg tggggatgcg agcgggtaag gcttgcaagc tttcgcgttt
    50461 ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg agtaatttat tcagcgtatc
    50521 gcgatattgc gcgaatttcg gatggtcaga tgcgtccatc atattgtcga tgaactgttg
    50581 cattggcggt tgcttaatac cttcgaggtt gtaaccgacg ctcatattaa agatgaacga
    50641 tttaccggaa tcagaaggct ggaacatcgc ttcgagaagg tgcagggcaa accaggcttt
    50701 gaggtattca tcccaggctt taagcagggt aaattcggta gaccattcgg tgttaaagca
    50761 ctcgtcttcg gcatcgatac agggcttttc cagctccagg cggtcaagaa tttggacggt
    50821 ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg acaatatttt gcgcgagctg
    50881 cgtgtgcggg ccagcggcag ggccgacggg agtggcacag gtttcaccga agacgctaac
    50941 agttttacct tttacaggtg agtaaaactg ttgctcggga ataccaaaga ttgagcgttg
    51001 ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca aacggaatgg gacgcataat
    51061 atcccccata accctctcct tagcttatac agatatcatt gagtgaccgg tgttgagaaa
    51121 tccggtggtt tagggggata gagcaacaat cgcaccactc ttgccaggtt tggcgggaaa
    51181 tataaaaaaa tgtgaggcgg ctcgaaactt aaaggctttt tgtgaatgaa ctcacaggtg
    51241 ccaaatgaga attaaaatca ctcctgaaaa ttattattgt cagcccattt atcacttttt
    51301 ctcttttctc aaaatcagtg attccttatc attatgataa agagtgctgg agagattaaa
    51361 tatttgaacg caatcgttta actacatttt ctaaatttta gtataccttt tttgcgctgt
    51421 aataaagtcg tccggagtat caatatctaa aattatttct gcattttcta tctcgacgga
    51481 ataatgagct cccataagga gtgcctgacg catattggtg acattgggtc gttggattgc
    51541 ctgcatcaag catggttttg acactaaaat cggatggccg gggatgccat tatggagtgg
    51601 cagtattgcg ccatcatttc gtaacgacca gatttttcta aaaatatcga tggtgagggt
    51661 tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc actgcgggta ctgcggcctt
    51721 tactgatgtt agtaaaccct gcgcataatc tgggttgtgg ataatagtga tattgctctg
    51781 gttcgcataa cgttcgtgca gttcattacc acgatagccg gtgactaaaa taattcggct
    51841 acaaaactgc aacgcatttt tgatacttgt atcaagaatt gtcccctgtt gccagggtaa
    51901 catcattttc cattgcccca ttcttgatga taatccggca gcggtaatta tacagtcgat
    51961 ggctgacata gtggttccat tatttatagg ggct
//
LOCUS       DAFAVF010000016        51543 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFAVF010000016 DAFAVF010000000
VERSION     DAFAVF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.016"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..995)
                     /locus_tag="LGK42_000913"
     CDS             complement(<1..995)
                     /locus_tag="LGK42_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBK2657189.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CRTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVGDRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCC"
     gene            complement(1007..2221)
                     /gene="rspA"
                     /locus_tag="LGK42_000914"
     CDS             complement(1007..2221)
                     /gene="rspA"
                     /locus_tag="LGK42_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBK2657190.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2427..2753)
                     /locus_tag="LGK42_000915"
     CDS             complement(2427..2753)
                     /locus_tag="LGK42_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBK2657191.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2888..3229
                     /locus_tag="LGK42_000916"
     CDS             2888..3229
                     /locus_tag="LGK42_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBK2657192.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3264..3824
                     /gene="speG"
                     /locus_tag="LGK42_000917"
     CDS             3264..3824
                     /gene="speG"
                     /locus_tag="LGK42_000917"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBK2657193.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3827..4537)
                     /locus_tag="LGK42_000918"
     CDS             complement(3827..4537)
                     /locus_tag="LGK42_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBK2657194.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4645..4950
                     /locus_tag="LGK42_000919"
     CDS             4645..4950
                     /locus_tag="LGK42_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBK2657195.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5149..7575
                     /locus_tag="LGK42_000920"
     CDS             5149..7575
                     /locus_tag="LGK42_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBK2657196.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7636..10059
                     /locus_tag="LGK42_000921"
     CDS             7636..10059
                     /locus_tag="LGK42_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBK2657197.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10070..10687
                     /gene="dmsB"
                     /locus_tag="LGK42_000922"
     CDS             10070..10687
                     /gene="dmsB"
                     /locus_tag="LGK42_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBK2657198.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10689..11543
                     /locus_tag="LGK42_000923"
     CDS             10689..11543
                     /locus_tag="LGK42_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBK2657199.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11586..12200
                     /gene="dmsD"
                     /locus_tag="LGK42_000924"
     CDS             11586..12200
                     /gene="dmsD"
                     /locus_tag="LGK42_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBK2657200.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12359..13651
                     /gene="clcB"
                     /locus_tag="LGK42_000925"
     CDS             12359..13651
                     /gene="clcB"
                     /locus_tag="LGK42_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBK2657201.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13604..14299)
                     /gene="bioD"
                     /locus_tag="LGK42_000926"
     CDS             complement(13604..14299)
                     /gene="bioD"
                     /locus_tag="LGK42_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBK2657202.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14424..15644)
                     /gene="mlc"
                     /locus_tag="LGK42_000927"
                     /gene_synonym="dgsA"
     CDS             complement(14424..15644)
                     /gene="mlc"
                     /locus_tag="LGK42_000927"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBK2657203.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15779..16672)
                     /locus_tag="LGK42_000928"
     CDS             complement(15779..16672)
                     /locus_tag="LGK42_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2657204.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16779..18032
                     /locus_tag="LGK42_000929"
     CDS             16779..18032
                     /locus_tag="LGK42_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657205.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            18429..18764
                     /locus_tag="LGK42_000930"
     CDS             18429..18764
                     /locus_tag="LGK42_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBK2657206.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            18857..18940
                     /locus_tag="LGK42_000931"
     CDS             18857..18940
                     /locus_tag="LGK42_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657207.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            19040..19861
                     /locus_tag="LGK42_000932"
     CDS             19040..19861
                     /locus_tag="LGK42_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBK2657208.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(19900..20229)
                     /gene="mdtI"
                     /locus_tag="LGK42_000933"
     CDS             complement(19900..20229)
                     /gene="mdtI"
                     /locus_tag="LGK42_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBK2657209.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20216..20581)
                     /gene="mdtJ"
                     /locus_tag="LGK42_000934"
     CDS             complement(20216..20581)
                     /gene="mdtJ"
                     /locus_tag="LGK42_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBK2657210.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            20993..22027
                     /gene="tqsA"
                     /locus_tag="LGK42_000935"
     CDS             20993..22027
                     /gene="tqsA"
                     /locus_tag="LGK42_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBK2657211.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     DVLLSDLNKE"
     gene            complement(22052..23440)
                     /gene="pntB"
                     /locus_tag="LGK42_000936"
     CDS             complement(22052..23440)
                     /gene="pntB"
                     /locus_tag="LGK42_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBK2657212.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23451..24983)
                     /gene="pntA"
                     /locus_tag="LGK42_000937"
     CDS             complement(23451..24983)
                     /gene="pntA"
                     /locus_tag="LGK42_000937"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBK2657213.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            25507..26451
                     /locus_tag="LGK42_000938"
     CDS             25507..26451
                     /locus_tag="LGK42_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2657214.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            26637..28019
                     /locus_tag="LGK42_000939"
     CDS             26637..28019
                     /locus_tag="LGK42_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2657215.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            28056..28778
                     /gene="folM"
                     /locus_tag="LGK42_000940"
     CDS             28056..28778
                     /gene="folM"
                     /locus_tag="LGK42_000940"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBK2657216.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(28775..29110)
                     /locus_tag="LGK42_000941"
     CDS             complement(28775..29110)
                     /locus_tag="LGK42_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBK2657217.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            29239..29958
                     /gene="rstA"
                     /locus_tag="LGK42_000942"
     CDS             29239..29958
                     /gene="rstA"
                     /locus_tag="LGK42_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBK2657218.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALNFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            29962..31263
                     /gene="rstB"
                     /locus_tag="LGK42_000943"
     CDS             29962..31263
                     /gene="rstB"
                     /locus_tag="LGK42_000943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBK2657219.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            31339..32268
                     /gene="tus"
                     /locus_tag="LGK42_000944"
     CDS             31339..32268
                     /gene="tus"
                     /locus_tag="LGK42_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBK2657220.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(32265..33668)
                     /gene="fumC"
                     /locus_tag="LGK42_000945"
     CDS             complement(32265..33668)
                     /gene="fumC"
                     /locus_tag="LGK42_000945"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBK2657221.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(33811..35457)
                     /gene="fumB"
                     /locus_tag="LGK42_000946"
     CDS             complement(33811..35457)
                     /gene="fumB"
                     /locus_tag="LGK42_000946"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBK2657222.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            35656..36831
                     /gene="manA"
                     /locus_tag="LGK42_000947"
     CDS             35656..36831
                     /gene="manA"
                     /locus_tag="LGK42_000947"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBK2657223.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            36932..38440
                     /locus_tag="LGK42_000948"
     CDS             36932..38440
                     /locus_tag="LGK42_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBK2657224.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPALY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(38666..39931)
                     /gene="uidC"
                     /locus_tag="LGK42_000949"
     CDS             complement(38666..39931)
                     /gene="uidC"
                     /locus_tag="LGK42_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBK2657225.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGGSVQKDVSNQIQSILEKGIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNDTA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(39970..41343)
                     /gene="uidB"
                     /locus_tag="LGK42_000950"
     CDS             complement(39970..41343)
                     /gene="uidB"
                     /locus_tag="LGK42_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBK2657226.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(41340..43151)
                     /gene="uidA"
                     /locus_tag="LGK42_000951"
     CDS             complement(41340..43151)
                     /gene="uidA"
                     /locus_tag="LGK42_000951"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBK2657227.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(43540..44130)
                     /gene="uidR"
                     /locus_tag="LGK42_000952"
     CDS             complement(43540..44130)
                     /gene="uidR"
                     /locus_tag="LGK42_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBK2657228.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(44359..45126)
                     /gene="hdhA"
                     /locus_tag="LGK42_000953"
     CDS             complement(44359..45126)
                     /gene="hdhA"
                     /locus_tag="LGK42_000953"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBK2657229.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(45238..46266)
                     /gene="malI"
                     /locus_tag="LGK42_000954"
     CDS             complement(45238..46266)
                     /gene="malI"
                     /locus_tag="LGK42_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBK2657230.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            46441..48033
                     /gene="malX"
                     /locus_tag="LGK42_000955"
     CDS             46441..48033
                     /gene="malX"
                     /locus_tag="LGK42_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBK2657231.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGIMPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            48043..49215
                     /gene="malY"
                     /locus_tag="LGK42_000956"
     CDS             48043..49215
                     /gene="malY"
                     /locus_tag="LGK42_000956"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBK2657232.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            49319..50320
                     /gene="add"
                     /locus_tag="LGK42_000957"
     CDS             49319..50320
                     /gene="add"
                     /locus_tag="LGK42_000957"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK2657233.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(50355..51395)
                     /locus_tag="LGK42_000958"
     CDS             complement(50355..51395)
                     /locus_tag="LGK42_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2657234.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
BASE COUNT        12761 a        13331 c        12531 g        12920 t
ORIGIN      
        1 ttgcagcaat gtttttgatc ctgttcaaat aaactaatgg catcagcaac atgctggaaa
       61 tcaaacgtat gggtaattaa tttttctggt ttaattaacc ctttacttaa ccagtcgata
      121 acaaccggaa atttatttgc atttaagcgt gaagagaaaa tagagagttc ttttccggta
      181 attccttgct gaatcacttc agacggttca ctggagaagc ccatcaatac aatacgtgcc
      241 gctggagaag ccagcgttac ggcctctttc aggatagaag gatgacaagc cgcatcgata
      301 attaatgtcg gcttgatgcc tttttcagcg aaaatctcgc caagcggtgt ctggctgtta
      361 ttaatcgccc tgtctgcccc gctctctttc gctttttcca gtcgttcatc aatgcgatcg
      421 ccaacaatca catttttaac gttatagacg ccttttaata cctgaacgat cgtcaggccg
      481 attggaccgg caccataaac cagaacggta tcattttcag tcggttgacc atgtccggtt
      541 acgttagccg caatggtaaa aggttcgatc atcaccgcat attgatcggc cactgcttca
      601 ggaattttcc acgcattttt tgccggaacc acggcatatt cactgaaacc accgtcagcg
      661 tgcacaccta atacagcaag tgtcctacaa acgttcggtt tacctataga gcacggatag
      721 caatgcccac agctgaccac cggatcgaca gcaacacgtt caccgactct ggcgctttcc
      781 acgccgtcac ccaccgcatc aatgacgcca aagaattcat gtccaatgac gcgcggatat
      841 ttcgcaaaag gattatgccc acgataaata tggctatcag aaccacaaat tccggcaagt
      901 ttcactttta ctcgtacttc acccgctgac ggggtgggta tttcacgttc gacaatcgcc
      961 agttgattcg gtttttcaat taatatgctt ttcattatct tactccttac cagttccaca
     1021 gcgtgccatc ttccagacgt gcgactggta gataagcagg ttcataggga tatttcgccg
     1081 ccagcttttc atcgaattcg atgccaagac ccggtttgtc tcccggatgc atatagccgt
     1141 tatcgaaagt ccagttgtgc gggaagactt cgagcatttg ttcggaataa cccatgtatt
     1201 cctggacacc gaaattgggg acccacagat caaagtgcag cgctgcagcc atgcagactg
     1261 gtgacaaatc ggaaggaccg tgtgagccag tacgtacctg atacagcgaa gcaaaatcgg
     1321 caatccggcg cataccggta attccgcctg catgggtcag cgtggtgcgg atataatcga
     1381 tgagttgctc ttcaatcagt tgtttgcagt cccagatgct gttgaagact tcacccactg
     1441 cgatgggtgt gacggtatgt tggcgaatga gacggaagca ttcctggttt tctgcaggcg
     1501 tcgggtcttc catccagaac atgcgataat cttcaatgct tttaccaaag cgcgccgctt
     1561 caataggcgt taagcgatgg tgcatgtcat gcagcaaatg ttcattaaaa ccaaacttgt
     1621 tacgtaccgc gtcaaacaat ttcggcatga aatcgaggta tttctccgtc gaccacagct
     1681 gctcttccgg ccactgtcct ttggttgcgg gttcataagc cagaccttta cctttcgaca
     1741 tgccgtaggt ggttttcata ccagggattc cgcactgcac gcggatggct ttgaatccca
     1801 gctcctgatg acgggcataa tcatccagag cttcatcaat actgtgaccg gtggtatggc
     1861 aataaaccat caccccttca cgagacgcgc cgccgagtaa ctggtaaagc ggcatgttgg
     1921 cagctttggc tttaatatcc cacagcgcca tatcaaccgc tgaaatggcc gacatcgtaa
     1981 ccggaccgcg acgccagtaa gcacctttat agaaaaactg ccagatatct tcgatacggt
     2041 gcgcatcgcg accaataagc tgcggacaaa ggtgatcctg caaataagag gccacggaaa
     2101 gctcacgtcc attgagggtg gcatccccaa ggcccgtaat accgtcctca gtggtgattt
     2161 ttaatgtgac gaaattacgc cccggacagg taacaaaaac ttcagccttt acgatcttca
     2221 tgttcgattc cttgcatcgc ttgtcgtgat gcatgaaatc tacgcaactg agctactacc
     2281 atacaagtat gaagatcgaa aaaagccgga gtgatcacaa aaaaaggcgt atatttgcgc
     2341 tgtgaatggt tgacaaaaga tgaaatagaa taccttttgt cagctgacac ttcctcttat
     2401 cttattgata aaatggattt atgttcctac gtgcgccccc agcccgcaac aatgatcaac
     2461 atgccgcaaa gcgcaatcaa cgcacccgtc cagtcataaa gagtcagttt cacgccatcc
     2521 acaacgcgca gccacatcaa cgccgtgcag acataaacgc caccataagc cgcgtaaaca
     2581 cgcccactcg ccgctggatg caacgttaac aaccagacaa acagcgccag tgaaatcccc
     2641 gccggaagca acagccagat actggcgttt cgttttaacc acaaccaggg cagaaagcat
     2701 ccaataattt cacacagcgc agtagcaaaa aatagtaacg ttgttttaat catctttgtc
     2761 acttattgac atcatgtata gttatagggc gacataatat catcaatata aacaccctcc
     2821 tggtacgttt tgcgttcgca gtggatggtg tagaatctcc tttaatcatt catacaggga
     2881 atgaattatg aaaatcactc tcagcaaacg aatcggcctg ctcgcttttc tgctgccttg
     2941 cgcactggca ctgagcacaa ctgttcatgc cgaaactaac aaactggtga ttgagtctgg
     3001 cgacagtgca caaagccgcc agcacgccgc tatggaaaaa gagcaatgga atgacacgcg
     3061 caatctgcgt cagaaagtga ataaacgcac tgaaaaagag tgggataaag ccgacgccgc
     3121 ttttgataac cgcgataaat gtgagcaaag cgccaacatc aatgcctact gggagccaaa
     3181 tactttgcgc tgcctggacc gtcgaactgg ccgcgttatt accccctaac ctgttattga
     3241 tttaaggaat gtaaggacac gttatgccaa gcgcccacag tgttaagcta cgcccgctgg
     3301 agcgtgaaga tttacgctat gtacatcaac tcgacaataa cgccagtgtg atgcgttact
     3361 ggtttgagga accctacgaa gcctttgttg aactctctga tctgtatgat aagcatattc
     3421 acgatcagag cgaacgacgc tttgtggtgg aatgtgacgg cgaaaaagcc ggtctggtgg
     3481 agctggtgga aattaaccat gtacatcgcc gggcagaatt tcagataatt atctccccgg
     3541 agtatcaggg gaaaggtctg gcaacccgtg ccgccaaatt agcaatggac tatggcttca
     3601 ccgttctcaa tctctataag ctgtatctga tcgtcgataa agagaatgaa aaagcgattc
     3661 acatttaccg caagcttggc tttacggttg aaggtgaatt gatgcacgag ttctttatta
     3721 atggtcaata tcgtaatgcc attcgcatgt gtatattcca gcatcagtat ctggcagagc
     3781 acaaaacacc gggtcagact ctcctgaagc cgaccgcaca atagcattaa taataatcga
     3841 tcgtattttt gatggtgtaa acccgttcga cggcgggttt tactccttca tcaacaatga
     3901 tcagctgaca gtccaccgga ttggcgtgac tgtcatattc acagctctgt tttacattac
     3961 caactcgttg attattcagt aaagtaaccg ctgtgtaatc taattttttg atcggatccg
     4021 ttgatggcgt ggcgctgaca gataatgttt tgtcgttact tttcgtggtt ttacccagcg
     4081 gataaccctg atcatcatag cgatattcca tctgcatttg tttgctgctg gctttaatca
     4141 cgaagccatt atcatcggtt tcccaactca ccccggcaga aggtaattct gctagctggc
     4201 attttccctg taaacgtact ctcttctcca gcgtctcggc atcacggtaa taattggcgt
     4261 ccagtaccag agcgaccacg gtattatttt ccagatccag taattcgagt gaatcaaaac
     4321 agccttcttc cgacaaagtc ccagaaacac gtttcgtcac ttcaccttgc tcatccatta
     4381 atgtctgagt gaaatctttt accggaccac gcagcggatc aaaatcgaat tcattagaga
     4441 aactggccat ctcaggggta aatgaaagtg ttacctctgt gcggtcacat cctgtgagga
     4501 atatcgcgag taagcatggt agtaatttgt atttcacaac agtcaccaga gagtagagat
     4561 gattctcaat catagtagca aatacagtac tttacacgtt aaatgctatg cttaaagaag
     4621 ttatcttcgc gtaaggagct tatgatgaaa ctctcaacct gctgtgccgc gcttctgctc
     4681 gccctcgcct cacccgcggt actcgccgcg ccgggatcct gtgagcgcat acagagcgat
     4741 atatcacagc gcattatcaa taatggcgta cccgaaagca gcttcacgtt aagtattgta
     4801 cccaatgacc aggttgatca gcctgattcc caggtcgtcg ggcattgtgc taatgatacg
     4861 cataaaattc tctacacccg caccaccagc ggtaacgtct ctgctcccgc gcagtctacc
     4921 caggatggcg cgcctgccga accgcagtaa tacattatcg actgaacgcc ggatacgaca
     4981 catccggcga tttgaacata caacataatc ccaccttatt actcataccc ttctattgat
     5041 atggattaat aattcttaac ccaaaatggg tagactccct ctattgttag cgcgctaaat
     5101 attcaatata taaacattta tataacgata aagaacaggg agtgagttat gtccaaaaat
     5161 gaccgaatgg tgggcatcag ccgcagaaca ctcgttaaat caaccgcgat aggttctctg
     5221 gcgctggctg caggcggttt ttctttaccg ttcaccctgc gcagtgcagc ggcagcggta
     5281 caacaggcca gcgaaaaagt gatctggggt gcctgttccg tcaactgtgg tagccgctgt
     5341 gcacttcgtc tacatgttaa agataatgaa gtgacctggg tggaaactga caataccggc
     5401 agcgatgagt acggcaacca tcaggtacgc gcctgtttgc gcggtcgctc catccgccgg
     5461 cgtattaatc atcccgatcg cttgaattac ccaatgaaac gcgtgggtac acgcggtgaa
     5521 ggcaaattcg aacggattag ctgggatgaa gccctggata ctatcgccag tagcctgaaa
     5581 aaaaccgtcg aacaatatgg caatgaggct gtatatattc agtactcttc ggggattgtt
     5641 ggcggcaata tgacccgctc ttcgccatca gcctcggcgg tcaaacgcct gatgaactgc
     5701 tacggcggtt cactcaatca gtatggctcc tacagcaccg cgcaaatttc ctgtgccatg
     5761 ccctacacct acggcagtaa tgatggcaac agcaccacgg atattgaaaa cagcaagctg
     5821 gtcgtgatgt ttggtaacaa cccggcagaa acccgcatga gcggcggtgg catcacttat
     5881 cttcttgaaa aagcgcgcga gaaatcgaac gccaaaatga ttgttatcga tccgcgatat
     5941 accgatacgg ctgctggtcg tgaagatgaa tggctcccta ttcgcccggg caccgatgcc
     6001 gcgctggttg cgggtattgc ctgggtattg attaacgaaa atctcgttga tcaacctttt
     6061 ctcgataaat actgcgtcgg ctatgacgaa aaaaccttac ccgcagatgc acccaaaaat
     6121 ggtcactata aagcctatat tcttggtgaa ggtgatgata acacagcgaa aacgccgcag
     6181 tgggcttcgc aaattaccgg tatcccggta gaccgtatca tcaaactggc gcgtgaaatt
     6241 ggcacagcaa aacccgctta tatctgccag ggctgggggc cgcaacgcca ggcaaacggc
     6301 gaactgactg cacgcgctat tgctatgtta cctattttga cgggcaatgt cggcatcagc
     6361 ggcggaaata gtggcgcgcg tgaatcgacc tataccatta ccatagaacg cctgccggtg
     6421 ctggataatc cggtcaaaac gtcaatctcc tgcttcagct ggacagatgc tatcgatcat
     6481 ggtccgcaaa tgacggcaat ccgcgacggc gtccgcggca aagataaact ggatgtgccc
     6541 attaagttca tctggaacta cgcgggaaat accctcgtta atcagcattc tgacatcaac
     6601 aaaacgcatg aaattctgca agacgaatcg aaatgcgaaa tgattgtggt catcgaaaac
     6661 tttatgacct catcggcaaa atatgccgac attctgctgc cagacctgat gaccgttgag
     6721 caggaagata ttattcctaa cgactacgcc ggtaacatgg gatatctcat tttcctccag
     6781 ccagtcacca gcgaaaaatt cgaacgtaaa ccgatttact ggatcctgag tgaagtcgcg
     6841 aaacgtcttg gacctgacgt ctatcaaaag ttcactgaag gtcgcacgca ggaacaatgg
     6901 ttacaacatc tgtacgccaa aatgcttgcc aaagatccgg cgttaccgtc ttacgacgaa
     6961 ctgaaaaaaa tgggtatcta taagcgtaaa gatcccaatg gtcattttgt cgcctacaaa
     7021 gcatttcgtg acgaccccga ggcaaatcca cttaaaacac cttccggtaa gattgaaatt
     7081 tattccagca agctggcgga aattgcccgt acctgggaac tggaaaaaga tgaagtgata
     7141 agcccactgc cagtttatgc ctctaccttt gaaggctggg actcccctga acgtagaacc
     7201 ttcccacttc aactgtttgg tttccattac aaatcccgta ctcactcgac ctacggcaat
     7261 attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat agatgcgcag
     7321 aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg cgaagttcgg
     7381 ctaccagcca aagtgacacc acgcattctc cctggggtta gcgctatggg ccagggagcc
     7441 tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt gaatacgcta
     7501 accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa tctggtcgag
     7561 atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg tttgaaaagc
     7621 aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc tgaaatcagc
     7681 cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag cagtgctttc
     7741 actctgccat tttctcaaat ggtccgggcg gcagaggctc cggtagaaga gaaagcggtc
     7801 tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca tgtgaaagat
     7861 gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg taatcatcag
     7921 gttcgcgcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc tgataggttg
     7981 aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggta aatttgaacg gataagttgg
     8041 gacgaagccc tggataccat cagtgataat cttcggcgga tcctaaaaga ttacggcaat
     8101 gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac caactcaaac
     8161 gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg cagctacagt
     8221 accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg caacagcccg
     8281 gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc agaaacgcgg
     8341 atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc aaacgcacgc
     8401 atgatcgtca tcgatccacg ttataacgac acagctgccg ggcgtgaaga tgaatggctg
     8461 cccattcgcc caggcaccga tggcgcactg gcctgtgcga ttgcctgggt actgattact
     8521 gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga tgaaaaaacg
     8581 ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg cgaagggcct
     8641 gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc ggcagaaaaa
     8701 attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg tcagggttgg
     8761 gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat gctttccgtt
     8821 ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg tagctgggat
     8881 ctgggggtag aatggttccc gatgcttgag aatcctgtta aaacgcagat ttccgtcttt
     8941 acatggacag atgccatcga ccatggtacg gaaatgaccg cgacccgtga tggtgttcgt
     9001 ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag taacacattg
     9061 atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga cagcaagtgc
     9121 gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg cgatatcctg
     9181 ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc tgcagggaat
     9241 atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg caaacccatc
     9301 tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca aacctttact
     9361 gaaggtcgca gtcagcatga atggatcaaa tatctccatg cgaaaacgaa ggaacgtaac
     9421 cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa aaaatgcccg
     9481 gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cacaggcaaa cccgttgaaa
     9541 acaccttcgg ggaaaatcga aatttattcg gaacgactgg cgaagattgc agatacctgg
     9601 gaattgaaaa aagatgaaat tattcatccc cttcctgcgt ataccccagg ttttgatggc
     9661 tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca ctacaaagcg
     9721 cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc acaagaggtg
     9781 tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac cgtgcgggta
     9841 tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat tctgcctggc
     9901 gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga ccgggtcgat
     9961 cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc aaagggaaac
    10021 ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca tgaccacaca
    10081 atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg aactggcgtg
    10141 caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg aatacgctgg
    10201 cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt attatctctc
    10261 catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca gcggcgcaat
    10321 gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg gctgccgcta
    10381 ctgccacatg gcttgtccgt acggcgcgcc acagtacaat gctgaaaaag ggcacatgac
    10441 gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat gtgtcgaatc
    10501 ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga agcacggcac
    10561 tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata tcgttatcaa
    10621 acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta atccggagga
    10681 agtgtaaaat gggaaatgga tggcatgagt ggccactggt tatctttacc gtattgggtc
    10741 aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc aaaaatgacg
    10801 cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg ggcatcgggt
    10861 ttattgcctc tgtcatgcac ctgggatcgc cactgcgagc gtttaactcg ctgaatcgaa
    10921 tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt gccgttggcg
    10981 gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg aaactctggt
    11041 tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc gtgtatcaaa
    11101 tcgacaccgt gccaacctgg cataacggtt acactacgct ggctttcttc ctgacggtat
    11161 tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agctcgcgtt acatttaata
    11221 ccacaccatt tgccatcatt agcgttttgg cgctgatcgc ttgtgccggg gtgattgtgc
    11281 tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc gcactggtac
    11341 cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt cttggatgct
    11401 ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt ctggggctaa
    11461 tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg cacatgacag
    11521 taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat ttcaggatgt
    11581 taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc gtgttgggcg
    11641 cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg gtactcacca
    11701 gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg ctggtgacgg
    11761 cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg tttgtcggcc
    11821 cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa tctgtgctgt
    11881 ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt cagtttgaaa
    11941 tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct gcgtggttgg
    12001 cggaaaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt tttccgtggt
    12061 caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac cgtgcactgg
    12121 gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt cctgtcgcgg
    12181 ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga ttacgcgcaa
    12241 tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg cactgctttt
    12301 gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat ttctgctcat
    12361 gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc ttatcgcaac
    12421 agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga tgctgctact
    12481 ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga caaacctttc
    12541 cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt tgttgctgat
    12601 gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca tggaagcgtt
    12661 gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg cctcgctgct
    12721 ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt tagctgccct
    12781 tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat tatggatcgc
    12841 ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg gcagtttatt
    12901 tatcgccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg tgattatttc
    12961 cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt tactctacaa
    13021 cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca gtacaggtgt
    13081 gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgcc atcgtggatt
    13141 tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcgggttga tcgtgggtct
    13201 gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac aatccttttt
    13261 aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac tgtgtgccgt
    13321 gctggcgagt agcggttccg gcgcacccgg tggggtcttt acaccgacgc tatttatcgg
    13381 tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg atggcgaaga
    13441 aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa ccacgcacgc
    13501 gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc tactccccgg
    13561 tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc gtgactctat
    13621 ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt tctcgctgtt
    13681 cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt ttacccagca
    13741 catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc acccagccaa
    13801 tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg ttaatgcaac
    13861 cttcctgaat accgacaacc atcaacaccg gcagttgttc ctgcaccacc cattcagaga
    13921 gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc acgacatgat
    13981 cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg attgggcaac
    14041 tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct tcataaggca
    14101 gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc ccttcgggtg
    14161 tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc tgggaggcta
    14221 acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct gtaccggtaa
    14281 taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat aaaccaggaa
    14341 aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga tagcgcaaat
    14401 tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa agaaccgtta
    14461 tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga aaactgagta
    14521 ctctcaacgc tgatgtgctg actatacgca ggaagggcct gctgacggat gctgtctgag
    14581 atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag tattttttgt
    14641 gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc caccccggta
    14701 atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga gtccacggtt
    14761 aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg tgccagctca
    14821 agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca ataacagcgt
    14881 ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct gcctgcgtgt
    14941 agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac cacctgaatc
    15001 acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca tgcgctgata
    15061 tcatgctgaa tataaaccgg aacgccggta tgctgctcca gagcctcgcc gagcggcatc
    15121 tcttttacat cctcgtagaa cggcatgcga tgtacaatgc cattttccgt atcaataatt
    15181 cccggcaggg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg gcggataaaa
    15241 aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt taacgccagt
    15301 tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag gaaaatctcc
    15361 ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac cagccccacc
    15421 gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag gtgtgcttcg
    15481 agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag acgggaaaga
    15541 tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc cgcgttggtc
    15601 tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata ctccctatat
    15661 tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt gtcgtcaaat
    15721 ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga aatttccctc
    15781 acctgagagc attcagcaga tgaatacgaa agtttcgcgc agctggactt tgttcatgat
    15841 gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttctgcaatc ggcacccagc
    15901 gcatttcgtt gagctgaaca cgtttaaatg acgcaggcaa aattgaaaca cccagaccgg
    15961 cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg acgggcgtca
    16021 aatggtaacg tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg acgtgcggat
    16081 caaaaaagac gaagggttct ttcgccagtt cagccagcgt tacattcggg ttatttgcca
    16141 ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact gcgtgttcaa
    16201 gcgtctccgg taacgctgtg ttacgcagca atcccatatc cagcgttcct tcaatgagcg
    16261 gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg ggataatcac
    16321 ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac gaagtaaaac
    16381 caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg gcagcgtcat
    16441 ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct gccgtcagca
    16501 atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc agtgcctgaa
    16561 tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc ccgaaatgca
    16621 gctcttccgc aacagcaaca aagtaacgca gatgacgaag ttcaatattc atatttaaaa
    16681 catcttattt gagattatta atatattaga cagaacaatt cgattttcct aacctatgta
    16741 taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact acaactgttg
    16801 atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat caatttatta
    16861 aacgcggtac gccgcaattt atgcgcgtca ccctggcgct gttctctgcc ggactggcaa
    16921 catttgcact tctctattgt gtgcagccta tccttccggt gctttcgcag gagtttggct
    16981 taacgcccgc gaacagtagt atttcactgt ccatttccac ggcgatgttg gctattggtt
    17041 tgctgtttac tggcccccta tccgatgcca ttggtcgcaa accagtgatg gtcacggcgc
    17101 tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg cacggcattt
    17161 tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt ggcatgactt
    17221 atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg tatatcagcg
    17281 gcaactcaat tggcggcatg agcgggcgct taattagtgg tgtcttcacg gactttttca
    17341 actggcgaat tgctctggcg gcaatcggtt gtttcgcgct agcctcggcg ttgatgttct
    17401 ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgacct aagacgttgt
    17461 ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc gcagaaggct
    17521 ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggctatcgg ttgatgcttt
    17581 ccccctggca tgtcagtcag gccgtggttg gcttattatc gctggcttat ttgaccggta
    17641 catggagctc acccaaagcc ggaaccatga ccacccgcta cgggcgcggt ccggtgatgt
    17701 tgttttcgac gggggttatg ctgtttggtt tactgatgac cttattcagc tcgctgtggc
    17761 tgatctttgc cggaatgtta ctcttctcag caggattctt cgcagcccac tcagttgcca
    17821 gcagctggat tggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg ctgtatctgt
    17881 tcagttacta tctggggtcg agtattgccg ggacgctggg tggtgttttc tggcataact
    17941 atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct ctgctggtcg
    18001 ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag cccggactgc ggtaactaca
    18061 cgtccggact tattgcttgc tcaaaccaac gttaatagcc atcctaaaat agacgaagcg
    18121 ccagccaatt cccgcagcgc gtctagcgtc atcaggatta taagtaccca aataaacgga
    18181 ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct ttgtaagtag
    18241 taaaatagtt aacccgatca agactactat tattggtagc taaatttccc ttaagtcaca
    18301 atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta cacgctgaaa
    18361 ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg gccccgcagt
    18421 ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta gctctggttg
    18481 ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg accacacctg
    18541 ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat aaaaaacagc
    18601 ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa aatacgaaag
    18661 ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc gggaaacaca
    18721 gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc aacagtaatg
    18781 ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt tagcaccgcc
    18841 tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta tcatccttat
    18901 tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat ctgattttac
    18961 tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta ataatcataa
    19021 ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg caattagttt
    19081 gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa acgatgaggt
    19141 cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga cccaatcacc
    19201 gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg cgacgctgat
    19261 tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa agggtaaagc
    19321 cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct atgagatcca
    19381 cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag atggggatgg
    19441 ttggattgtt cctcccgcag ctgcaccatg ggacttcggc ttgattgtgc tgcgtaatcc
    19501 cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc ttactgccgc
    19561 attaaaatcg gctggtcgta aagtgactca ggcgggatat cctgaagatc atctcgatac
    19621 gttatacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg tgatgtcaca
    19681 tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata ccgatgacgg
    19741 ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct ggcgcgccga
    19801 taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt cgcaaaaata
    19861 atgttcaaac gctgcccgac agcgcgggca gcgtcttcat caggcaagtt tcaccatgat
    19921 cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc gttgaccaaa
    19981 caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca atgcataagc
    20041 gacagacaag tcgatccctt ttacggcctg agaaagcgca ctaaaggcag ccagcaccgc
    20101 cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt tcaaaaagac
    20161 gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa cccattcaaa
    20221 ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct gattttatca
    20281 acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat aaactttcgt
    20341 cgaataacaa aacgctgaac aaggtaataa ataaaatacc gataccttcc cacaacgcat
    20401 aagctacgcc taaggcgatt tttttaacgg cgaaagagag aaatatatac gacagagaaa
    20461 tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc catttcattg
    20521 acagcgtacc ggtaatttct gtagcaatag ccagacctaa taaaatccaa taaatataca
    20581 ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga aaccaaagta
    20641 aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta aagcgcgttg
    20701 cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg ggtaaatgta
    20761 gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt gttgcttctc
    20821 gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta tcatagctta
    20881 agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc gttatgatga
    20941 cggagcgaaa aagacattat tattagcaaa ggaagaaaaa ccggggacaa gcatggcaaa
    21001 gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc tggtcattat
    21061 tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg cattatttat
    21121 tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc cgcgtgtact
    21181 ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat tagcttatct
    21241 gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact ctattatgac
    21301 gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct cagttgacca
    21361 gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact tattgacgca
    21421 gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt ttatgctgct
    21481 cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg ttgaagggat
    21541 ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga aaacagccat
    21601 cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg ttcgcttcgc
    21661 ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg gttcagttct
    21721 cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg aagcgttgct
    21781 ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg agccgcgcat
    21841 catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga ttttttgggg
    21901 atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta ttgtcaaaat
    21961 tgcgcttgaa caaacagcgg gaggtcaaag catcgacgtt ctgttaagcg atctcaataa
    22021 agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt gcatccacgc
    22081 tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc gggttttgca
    22141 caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc gctttccaca
    22201 cttccagaac aggcatacca gcaataggac tcttcggatc atcctgcgcc gccgggttaa
    22261 ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca tcgttgatct
    22321 cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat acgttcatat
    22381 gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata ccacgagcgc
    22441 gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc atgccgtacc
    22501 ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct tctgcggtga
    22561 tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg gtgccgaaac
    22621 cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg taagaaagga
    22681 tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg ctgagcataa
    22741 agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc accactggca
    22801 tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca atggcggtca
    22861 ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat acaatcagca
    22921 gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc agcatcaacg
    22981 gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc gtgaacgtta
    23041 ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc agaatcggtg
    23101 ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc gccagaccca
    23161 cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact ttcttcgcca
    23221 gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca acattacccg
    23281 tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata ccgaagttgt
    23341 taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata aacaggatcg
    23401 cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc ttaatttttg
    23461 cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt aatactggct
    23521 ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat ctgcaacagt
    23581 gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc
    23641 gcatgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc gaaaacggtg
    23701 aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag gatgattgcc
    23761 agcgccatca atgcgtattt acgccacggt gagcaggcac atttttcctc agttttcact
    23821 tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg cggtgccggc
    23881 caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc atcaaaatca
    23941 acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac gaggttagta
    24001 ccgtaaagct gtgaggattg cgtcggcaga cggcccggaa gatcggtata accaatcact
    24061 ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca gttgccaccg
    24121 ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac catttcacgg
    24181 gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat gatatcgacc
    24241 tcttttgcct gggcggcaaa gagttccatt tccgctttga tgaacgcgtc cgacatcact
    24301 ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag gaattccgcg
    24361 cccatacttt gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg cacaatcgcg
    24421 ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc aatcaccatc
    24481 acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa gcgcccaaat
    24541 tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga gcttagtgcg
    24601 tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac ggtcacgtta
    24661 cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa actcaccagc
    24721 gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac cttcagaatg
    24781 atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg cacaaacgct
    24841 ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt aaaacccagt
    24901 ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt ggttaaccgt
    24961 tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga tgatggtttg
    25021 cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc cgttaaaata
    25081 aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa
    25141 tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag tgggataaac
    25201 accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa aacgagacat
    25261 cgccccggca cgaatcacta cttaacatta aattaactta tacaattcag ttgcttcagt
    25321 agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc aggattaaaa
    25381 ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt aaatgcaata
    25441 atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag gcgaaggatt
    25501 atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc tacggcattc
    25561 tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa accttttgac
    25621 cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc cgtttctcgt
    25681 cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga ttttggtaac
    25741 agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa agcagaagaa
    25801 acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc tgttctgatt
    25861 gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga agtcaatgat
    25921 gccatcacca aagcagcaaa agcgaaaggt gcctactctt tctacattgt tcgtcaaatc
    25981 gatgccaacc agggcggcaa ccagcgtatt actgcattca tctataaaaa agatgctaag
    26041 aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg acgtgcagct
    26101 ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc caggtgttgc gactaccgca
    26161 tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg cggacgttac
    26221 actgtcacgc ttcctgacgg cactaaagtc gaagaactga acaaagcgac agcagcgatg
    26281 atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac cgaagtctcc
    26341 tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat cacccgccag
    26401 tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata actattcagt
    26461 tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat ttccccgcga
    26521 ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa actccatttt
    26581 tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg atatcgatgg
    26641 aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg ctgggcgcgg
    26701 gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca ctgctcatcg
    26761 gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg atcctcacgc
    26821 gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg tttggcgagc
    26881 taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc aacgtctcct
    26941 atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa ttgcgcctgt
    27001 tcggcgatgg taatacctgg caatcgatag tcggtgcatc ggctttattg tggattgttc
    27061 actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg gcgacactgg
    27121 caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc aaactggata
    27181 ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtgtgggaa caggtgaaaa
    27241 acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg gttgtttctg
    27301 cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt ctctccgctc
    27361 tgggcgttta cttgctggta acgctgcttt cgctgggcgt ggtggcacgt cctgaactgg
    27421 ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga ccatggggcg
    27481 aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta agctggacca
    27541 tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca cgcatttttg
    27601 cgcgccagaa cgcacaagct gcgccatccg cctcgctgtg gttgaccaac atctgcgtgc
    27661 aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg ctgaccatcg
    27721 cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg aaaatcgcaa
    27781 cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta tggttattgt
    27841 atgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg gggctgctgg
    27901 ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt caggaaatgg
    27961 tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg gtgggataac
    28021 gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag cccttgccaa
    28081 tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcatggcat ttcattaatc
    28141 aaaagcaacc ggtgattgtc agctatcgga cacactatcc agccattgat ggactgatta
    28201 atgcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg atggcgtttg
    28261 ccgatgaagt actaaaaagc acccatggtc tgcgtgctat tttgcataac gccagtgcgt
    28321 ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg atgcagatcc
    28381 acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt gggcacggac
    28441 acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt agcgacaaac
    28501 atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg tttgcccgca
    28561 agctggcacc ggaagtgaaa gtgaattcta ttgcgccatc gctgatcctg tttaatgaac
    28621 atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa accgctcctg
    28681 gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgtttt gtcaccggac
    28741 gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc cagcatataa
    28801 tcaacaccca tgcgcttatc ccccaacacg caacagaacc aacaaaagcg gcgggcaagc
    28861 gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga ataatcgacc
    28921 acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg gcaacaatat
    28981 aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata taataatttt
    29041 tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg gctttgatta
    29101 ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa tacctgatga
    29161 aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg tgacgttttt
    29221 atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc ggaagtcggt
    29281 tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga gccgcgcggc
    29341 gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact cgacatcatg
    29401 ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg gtctggaccg
    29461 attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact ggaaatgggt
    29521 gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg tttacgtttg
    29581 catttgcgtc agaatgagca agccacactg agcaaaggtc ttcaggaaac gtctctgact
    29641 ccctacaaag ccctgaattt cggcacgttg accatcgatc ccatcaaccg cgtagtcacc
    29701 ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg ggaattagct
    29761 acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg cggcgtcagt
    29821 tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa aaaactgctc
    29881 gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta tctttttgcg
    29941 cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct gttattgttt
    30001 gtctgcttcc ttgtgatgtc tctgctggtt gggttggtgt acaaatttac cgccgaacgc
    30061 gcgggcaaac agtcgctgga tgatttgatg aacagttcgc tgtatctgat gcgcagcgaa
    30121 ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga tttaaatctc
    30181 tctttcgatc tgcgtgtcga gccattgagt aaataccatc ttgatgatat ttccatgcac
    30241 cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt gcagcgtatc
    30301 ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta tctccatcag
    30361 atgcgattgc tggatatcgc cctgatcgct tttattgcta tttccctcgc ctttccggtg
    30421 tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc ggcgcaacga
    30481 tttggcgatg ggcatctcaa tgagcgtatc cactttgatg agggttcgag ctttgaacga
    30541 cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc cagcaaaaaa
    30601 cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga
    30661 ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg tgatatcagt
    30721 caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg cccacaaaac
    30781 gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct ggcagacatt
    30841 caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg ccattatgcg
    30901 gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa cgccctgcgg
    30961 tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc gacattaatt
    31021 gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt tgaacctttt
    31081 gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg gctggcaatt
    31141 gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag cgaactgggt
    31201 ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt tacctctgcc
    31261 tgacactacg cgcacgatgg tcaagtcacc acgactgtgc tataaaataa gtatgttgta
    31321 actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa cactaccttt
    31381 cgccagatgg aacaagagct ggctacattt gccgctcatc ttgagcaaca caagctattg
    31441 gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa tccgcttaat
    31501 cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt gcgtcatttc
    31561 cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc tgtccgtctg
    31621 cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt cagtcatatt
    31681 cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga atcagaactc
    31741 cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat cacccttaat
    31801 gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg ttgggctaat
    31861 aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg
    31921 aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa actggagcga
    31981 gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa acgtccggtg
    32041 aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt ccaacacgcc
    32101 tgccctacac cactgattgc actgattaat cgggataatg gcgcgggcgt gccggacgtt
    32161 ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc acaggcgcag
    32221 ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cagattaacg cccggctttc
    32281 atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc gctaagatac
    32341 cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt tttggcgatc
    32401 tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat cagcgattca
    32461 ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca gtgtttgtta
    32521 aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg gatcaccatt
    32581 ggacggaaga cgttcagttc aaagttaccg gaagcgcccc ccatgttgat cgccacgtcg
    32641 ttccccatca cctgacagca gagcatggtt aatgcctcac actgtgttgg attcactttc
    32701 cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc aattccgcag
    32761 cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga cacagccaac
    32821 cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgccagcgc ttcaaatttg
    32881 ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc tacgcgacgc
    32941 gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccaag agccagttcc
    33001 gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat cgctacccag
    33061 ccggaaatct cctgccctag tgttagcggc gtggcgtcct gcaagtgggt tcgaccgatt
    33121 ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag ggttttaagc
    33181 tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc cgtcggaaag
    33241 acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt acgttccatc
    33301 ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt catgttcatg
    33361 ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc gtcatgctgt
    33421 cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc agacaacaag
    33481 cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc atgaatcagt
    33541 gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg
    33601 ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc gctgcgtact
    33661 gtattcatgg cctgctcctc acctgtttaa ttttttcttt ctgttttgct ttcgttaagc
    33721 aacttttagc tcacttatta tttactattt gataacaaat gtttggtttt tcgtgccatg
    33781 taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc actgtgtgag
    33841 ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg ccgggaaatc
    33901 ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa cacattccag
    33961 gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc caaggtagaa
    34021 gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt tcgccagcat
    34081 gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc gtccggcggt
    34141 cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac ccgcgtagta
    34201 aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc gctctttcag
    34261 tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata agcgtgtaga
    34321 aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg
    34381 caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac ctggattatg
    34441 ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat tacggtcagc
    34501 agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca ggcgaatcac
    34561 gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga gattttgcgc
    34621 ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt gctcattccc
    34681 ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt taaggttagt
    34741 ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg ccgccgtacc
    34801 cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta gtaacgcttt
    34861 ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga tacagaggaa
    34921 tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat tggtgccggt
    34981 attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat tatcttcgat
    35041 ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg tccatacacg
    35101 ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg gcagaacacc
    35161 tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat cattttcgct
    35221 ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac gcagcataaa
    35281 tgacgcatca tgaaatgcct gacgcgccaa cagagttaac gcttcgggtg cgactttcaa
    35341 aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca ggtaatactc
    35401 agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt ttgacattgt
    35461 tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg ccctgttaaa
    35521 agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta cgactttttg
    35581 cggctccagg ttacttcccg taggattctt gctttaatag cgggattaat ttccacatta
    35641 aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc ctggggcagc
    35701 aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc gatggccgag
    35761 ctgtggatgg gcgcacatcc gaaaagcagt tcacgggtgc agaatgccgc cggagatatc
    35821 gtttcactgc gtgatgtgat tgagagtaat aaatcgactc tgctcggaga ggccgttgcc
    35881 aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca gccgctctcc
    35941 attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga aaatgccgca
    36001 ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa gccggagctg
    36061 gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc cgagattgtc
    36121 tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt acaacagcct
    36181 aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg tgaagaaaaa
    36241 tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga accgtggcaa
    36301 acgattcgtt taatttctga attttacccg gaagacagcg gcctgttctc cccgctgtta
    36361 ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac
    36421 gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt gctgcgtgcg
    36481 ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa attcgaagcc
    36541 aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact ggacttcccg
    36601 attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga aaccaccatt
    36661 agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt gtggaaaggt
    36721 tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa cgaatcaccg
    36781 gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta agagcttact
    36841 gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc aggcggccag
    36901 tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag cggtaggcgt
    36961 cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca agaagattga
    37021 aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga cagctcctga
    37081 atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca gccagttgca
    37141 actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg gtcagagcgt
    37201 catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta aaaaactgaa
    37261 cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag taagcaaacc
    37321 actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca ttggttacag
    37381 cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa aggatgaaaa
    37441 agtcgccttt agcggcggcg agttccagtt aaatgcggac agagacggca aagctatctc
    37501 cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca accagaaagt
    37561 gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa gttttggtga
    37621 gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcggtgg aaggcaaaga
    37681 actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca atgatggtaa
    37741 aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga atcaggatct
    37801 gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct ggcatcagtt
    37861 tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa ttgccaacaa
    37921 tcccgcactt tatcaggaga aagtgacgga agccttcttt agcgccctgc cgctgatgtt
    37981 gaaaggcgat ccggtgatta ctatcgcacc gctaagctgg aaaaacagtc agggtgaaag
    38041 tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag cgccgcaaac
    38101 gctggcgcag gaagtggacc gctcggttaa atctctggat gcgaaactga ccattccggt
    38161 ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc aggaagatca
    38221 agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc agatgttccg
    38281 tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatgcta acggtcagat
    38341 aacgttaaat gggcagaaaa tgtcgttaga agattttgtt ggcatgtttg cgatgccagc
    38401 tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacgacca aaacgccgga
    38461 gaattttcac tccggcgttt tcatctgaaa acaaaccaga gattgcctga tgcgctacgc
    38521 ttatcaggcc tacctttacc tgcaacactt tgaatttatg agtttttgta ggctggataa
    38581 ggcgtttacg ccgcatccgg cataaaaaac gcgcactttg tcaacaatct gaaacgccgg
    38641 aggtttttct ctccggcgtt atttcttact tcagcataaa gtcatactga atactcattt
    38701 tcacatccca gtaattcagg cgatcgccca gcgcactgcc atcgcgcttt tcccgccctt
    38761 tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca
    38821 taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat tcatagccgc
    38881 gagtcacaat cggtgcaaat gtcagtgtaa attgcggcgt taaacgatag ttgacgccca
    38941 attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa
    39001 tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata
    39061 cgcttcccca cggcgcattc caggtcactt gtccactaac caacgccgta tcattgggct
    39121 ggctggtatt acgacttaaa ccttcgagtt gcgcgtaaaa gccgagcaac tcccctttca
    39181 acgtataatt cgggtcgagc ggtatgcctt tctcgagaat actttgtatc tgattggaaa
    39241 catctttctg taccgatccc cccagagaat agcgactggt atcatcatgc caactggcgg
    39301 ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt
    39361 catcacgaat accggttgtt gaatcggtcc ataaaaatac tcgtgaacgc cacattcccc
    39421 acagatcaat atttggcgtt aacgcccaat aacctaatgc tccttcagaa actgtcggta
    39481 atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta ccgtaactat
    39541 aatccgtccc gaagcgacct aatttaagca ggctattttc actgggtttt atttttactg
    39601 cccctagggc aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag
    39661 cacgagcacc taatttataa acataatacg ccccgccttc aacgccaata atattggaat
    39721 aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg
    39781 cgccagcact gggcttatca gccctgcgca attcgttttt cagtctgatg cgtagtcccg
    39841 cttcatcgtc agccagttgc gccgcataag cagaggtaag gccagaggca gccgtcaggc
    39901 aaataacggc catggccact atttttctca tagttgcacc tttgatgttc tgattatttt
    39961 tattgaatat taattagtga tatcgctgat taattgctgc tgcacttttt tacgattatc
    40021 aatttcaacc acgatttctt tgaatttttt atccgtgagc ggataaaacc agataataac
    40081 gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat
    40141 aacttccggc gtttgcacct gattggcgat atatccgctt aatcccaaaa taaaggcagg
    40201 aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag
    40261 cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag cttccagtgc
    40321 ccacatcaca gtcatagtaa cgccctgacc aattgaagcg atggccaacg caaccaacgc
    40381 caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg ttcccagcaa
    40441 agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg gcaccagcgg
    40501 tgccgatgcc acagtaccga ccaggttttg caccagtacc agcacagtaa acagcccggt
    40561 atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctaaccgcga aggtcgaaat
    40621 cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt tcagggtttg
    40681 cagactgatc ttcaatgacg gctgcgccac gatacgtacc acattctcac gcgtcgattt
    40741 gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc agaaatggta
    40801 taccgacacc atctcttccg ggctggagtt cttaatgctc ggtcctatca gaaatgccag
    40861 gcagacaaag gttaatgaag cggcaatccc gcgagccgcg cccagacggg cgcgggattg
    40921 tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca ggctgtagca
    40981 aagcccgagg cccatgtagg tcaaatatgc atacaccact ttgctaccat ggctccagtc
    41041 ggtcggcacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa
    41101 aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc cggcaaagac
    41161 gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg cagcggcacc
    41221 gacgccagcg acgtcggtgt agtaactcaa taggaagagc gcccccattg cgaaggcgaa
    41281 gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga gttgttgatt
    41341 cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc
    41401 agcagaaaag ccgccgactt cggtttgcgg tcgcgagtga agatcccttt cttgttaccg
    41461 ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg
    41521 acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac
    41581 tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat
    41641 tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt
    41701 accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg
    41761 cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg
    41821 acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa
    41881 tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg
    41941 cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga
    42001 gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa
    42061 ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca
    42121 tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag
    42181 ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct
    42241 ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg
    42301 ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag
    42361 atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc
    42421 ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca
    42481 accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc caccacctgc
    42541 cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc
    42601 acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc atagttaaag
    42661 aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc
    42721 gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg
    42781 gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc
    42841 acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg
    42901 atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc
    42961 gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt
    43021 gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga
    43081 tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca
    43141 ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt taatctgatt
    43201 agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa gagaaatgga
    43261 aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca cagcgtgata
    43321 ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc aaccgaacgt
    43381 tcgttcgagt atattgggaa cgataaactc tcatgacata tggctacggt gaatattttt
    43441 gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt aaagtatgaa
    43501 acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg ttaagatacc
    43561 gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat cttccgcttc
    43621 gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca ttgacgtgtt
    43681 gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg caacaaattc
    43741 agtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac gcatcccttc
    43801 cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga gagagacaat
    43861 agactcgacc atatagtcaa caaaatgaat cccttcaatc ggttcccgga aacgggccag
    43921 cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg agatgaaatg
    43981 gtgatagagc gtcccgggac taatggcgca ggatttacag atggctttca tcgaggcact
    44041 gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg tccgtgtcgg
    44101 ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt gaataactag
    44161 tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa ggcacactct
    44221 attcgtatgc ttttcatcca tctattggtt aaccaatagc cgtgatttgg ctgccagttg
    44281 tttagcagag acgttgatga attgcacacg ctatcccggt aaacaacaca tacggctgtt
    44341 taccggttcg ttagtgtatt aattgagctc ctgtacccca ccaccggaga cggtgagaat
    44401 ttgtccgctt acccagctgg ctgcaggcga gcaaaggaac agcgccgcgt tagcaatatc
    44461 ttgcggttgg cccagacgtc tgatcggcgt gtgctgtaac attttttgtt caatttctgg
    44521 tgtaataacg gatttcaggg catcggttaa tattgccccc ggcgcaatgc cattcacccg
    44581 aatatttttt tcacccaggt caaatgccat atttctgacc agatgactgg ccgcagcttt
    44641 agatgatgca taggaagtca tgtttatatt tttattttct gccgccatag aagtgatggt
    44701 cagaataacg ccaccgccat ttttttccat ttctggcgca acaagttgtg acagatggaa
    44761 aaaagaaaac acattcagtt cataagcacg gcgaaaatct gccattggca tatcaaacgg
    44821 tttaggacca ccgccaccgg cgttgttaac caggatatca actttaccca gcttactgac
    44881 ggcaaagtct gccagtgcag agagttcctg ttcggaagta atatcacaac ggcaggcaaa
    44941 tgcctgacca cccagttgtt gaatttcgtc tacaacatgg ttagctgcgt cggcgttaat
    45001 atcactgacc accacagatg cgccagctgt cgcgaatgta atggcgattt ctttaccaat
    45061 acctgcaccc gcacctgtga tgatggcgca ttttccgtcg agtctcaggt tgtcagaatt
    45121 aaacacataa acctcctgtg aacttcagta aaaataaaag gcaccgcctg tgtgccacat
    45181 tttttatcat taaccttgag gaaaggacac taccaggtat agcagataaa gagaggatta
    45241 tttcgccgca atgagccggg cgggaataat aagattgcgt gaatgcgtct cttcatgggt
    45301 gattttttgc atcatgcgat ccgcaagtgt ggtaccaagt tcccgcgcag gcgtgctggc
    45361 ccaggtaacg ggtatatcat caagtgtggt tggtgtcgca tcggtaaatg ccgccagcga
    45421 aacttgttgc tcaaagtaac gatcgactcc gctttccccg ctttgccgcc ctgctttgag
    45481 caaaccaaac catgccccca tcgcaatagt ttcgttatag caaaccacag cactgatggt
    45541 cgggttatga cgtaaaagcg ccgtgatagc ttccgcggct tgcttctggc tggatgtgca
    45601 ctccagcacc caatcgctgt gaaacggcag gccaaatttt agtagagttg cacaatagcc
    45661 ccccacccgt tctgcacggg ttaatgagga actttgccct cccagccagg cgatccgctg
    45721 atgcccattg cgaatgagat gctccgtcaa caactgtgca gcctgcatgt tgtccgggcg
    45781 aaccgtatca acatcatcaa gataactggc acgggaagcg aaaatcaccg ggatagcttt
    45841 ttcttctgcc atccgtcgca ggtcatcgct gcttcctgca gccccggcaa ttaccacacc
    45901 atccacaccc tgattcagta acagtgaaaa ccgctgtgcc agctgctcgc cgtctttacc
    45961 gccgtgaagc aaaaaaacca tccgtccctg cgcttccaga gcttccgtca atccggccgt
    46021 caattcggcg taaaacggcg cagataaatc acggacgatc aaaccaatga cgccgctttg
    46081 cccgccgcgc agcgccgacg cctggcgatt gcgcacaaat cccagctctt caatggcggc
    46141 gttaacgcgt tctcctgtgg cggtagagat tcgccctttg ccactaagca ccagcgaaac
    46201 ggtacttacc gacacgcccg cagccagcgc aacatcatga atggttattt ttttggcggt
    46261 agccatgcag atgacctact ccctgattat gtgacagata aaacgtttta ccttttattt
    46321 atcttatacc cgctattatc gttgcgtaat gtgatttatg cctcactaaa atttgataaa
    46381 acgttttatc ttctcgcgca atttactgaa tccagattgt tctctacgag gagtcgtttt
    46441 atgacggcga aaacagcacc gaaagtcacg ctgtgggagt tcttccagca gttaggcaaa
    46501 accttcatgt tacccgtggc attattgtcg ttctgcggca ttatgctcgg cattggtagt
    46561 tctcttagca gccatgatgt cataaccctg atcccggtcc tgggcaaccc cgtgttgcag
    46621 gctatcttta cctggatgag taagattggc tcgtttgctt ttagtttcct gcctgtcatg
    46681 ttctgtatcg ccatcccgct gggcctggca cgcgaaaata aaggcgtagc ggcattcgct
    46741 ggcttcgtcg gttatgcggt aatgaacctc gcggtaaact tctggttgac caataaaggc
    46801 attatgccaa ccacggatgc cgcggttctg aaagccaata acatccagag cattcttggg
    46861 atccagtcga tcgataccgg gatcctcggt gcggtgatcg ccggtattat cgtctggatg
    46921 ctgcatgagc gtttccataa tatccgcctg ccggatgcgc tggcattctt cggcggtacg
    46981 cgcttcgtac caattatctc ctcgctggtg atgggcctta tcggcctggt gattccatta
    47041 gtctggccga ttttcgccat gggtattagc ggcttgggcc atatgataaa cagcgcgggt
    47101 gatttcggac cgatgctgtt tggtaccggt gaacgtctgc tgttgccgtt tggtctgcat
    47161 cacattctgg tggcattaat tcgctttacc gacgcaggcg gcacgcagga agtctgcggt
    47221 caaaccgtca gcggcgcact gaccatcttc caggcgcaat tgagttgccc gaccactcac
    47281 ggtttttctg aaagcgccac gcgtttcctt tcgcaaggta aaatgcctgc gtttctcggc
    47341 ggtctgccag gtgcagcgtt agctatgtat cactgcgcgc gcccggaaaa tcgccataaa
    47401 attaaaggtc tgctgatttc tggcctgatc gcctgtgtcg ttggcggcac gaccgaaccg
    47461 ctggaattcc tgttcctgtt cgtggcgcca gttctgtatg tcatccacgc gctgttaacc
    47521 ggcctcggct tcactgtcat gtctgtgctc ggcgtcacca tcggtaatac cgacggcaat
    47581 atcatcgact tcgtggtgtt cggtattttg catggtctgt caaccaagtg gtacatggtg
    47641 ccagtggtgg cggcaatctg gtttgtcgtt tactacgtca tcttccgttt cgctatcacc
    47701 cgcttcaatc tgaaaacccc ggggcgcgat agcgaagttg ccagctcaat cgaaaaagcc
    47761 gttgccggtg cgccgggtaa atcaggttac aacgttcctg caatcctcga agcattaggc
    47821 ggtgccgaca atattgtcag cctcgataac tgcattaccc gtctgcgttt gtctgtgaaa
    47881 gatatgtcgc ttgttaatgt gcaggcactg aaggacaatc gggcaattgg cgtagtacaa
    47941 cttaatcaac ataacctgca ggttgttatc gggccacaag ttcagtcagt aaaagatgaa
    48001 atggccggtc tgatgcatac tgtccaggca taaggataag atatgttcga tttttcaaag
    48061 gtcgtggatc gtcatggcac atggtgtaca cagtgggatt atgtcgctga ccgtttcggc
    48121 actgctgacc tgttaccgtt cacgatttca gacatggatt ttgccactgc cccctgcatt
    48181 atcgaggcgc tgaatcagcg cctgatgcac ggcgtatttg gctacagccg ctggaaaaac
    48241 gatgagtttc tcgcggctat tgcccactgg ttttccaccc agcattacac cgcaattgat
    48301 acgcagacgg tggtgtatgg cccttctgtc atctatatgg tttcagaact gattcgtcag
    48361 tggtctgaaa caggtgaagg cgtggtgatc cacacgcccg cctatgacgc attttacaag
    48421 gccattgaag gtaaccagcg cacagtaatg cccgttgctt tagagaagca ggctgacgac
    48481 tggttttgcg atatgggcaa gctggaagcc gtgttggcga aaccagaatg taaaattatg
    48541 ctcctgtgta gcccacagaa tcctaccggg aaagtgtgga cgtgcgatga gctggagatc
    48601 atggctgacc tgtgcgagcg tcatggtgtg cgggttattt ccgatgaaat ccatatggat
    48661 atggtctggg gcgagcagcc gcatattccc tggagtaatg tggcgcgggg agactgggcg
    48721 ttgctgacgt cgggctcgaa aagtttcaat attcccgcgc tgaccggtgc ttacgggatt
    48781 atagaaaata gcagcagccg tgatgcctat ttatcggcac tgaaaggacg tgatgggctt
    48841 tcttcccctt cggtactggc gttaactgcc catatcgccg cctatcagca aggtgcaccg
    48901 tggctggatg ccttacgagt ctatctgaaa gataacctga cgtatatctc agataaaatg
    48961 aacgccgcgt ttcctgaact caactggcag atcccgcaat ccacttatct ggcctggctt
    49021 gatctacgtc cgttgaatat tgacgacaac gcgttgcaaa aagcgcttat cgagcaagaa
    49081 aaagtcgcga tcatgccggg gtatacctat ggtgaagaag gtcgtggttt tgtccgtctc
    49141 aatgccggct gcccacgttc gaaactggaa aaaggtgtgg ctggattaat aaacgccatc
    49201 cgcgctgttc gctaacccca attgcgcaac gtaaaaaatc gttgcgcaat cgtggatttt
    49261 taccctgctt tgtttttata atggtgcgca cttttatatt cagaaaaaga gtgcgaccat
    49321 gattgatacc accctgccat taactgatat ccatcgccac cttgatggca acattcgtcc
    49381 ccagaccatt cttgaacttg gccgccagta taatatctcg cttcctgcac aatccctgga
    49441 aacactgatt ccccacgttc aggtcattgc caacgaaccc gatctggtga gctttctgac
    49501 caaacttgac tggggcgtta aagttctcgc ctctcttgat gcctgtcgcc gcgtggcatt
    49561 tgaaaacatt gaagatgcag cccgtcacgg cctgcactat gtcgagctgc gtttttcacc
    49621 aggctacatg gcaatggcac atcagctgcc tgtagcgggt gttgtcgaag cggtgatcga
    49681 tggcgtacgt gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg gcattatgag
    49741 ccggaccttc ggcgaagccg cctgtcagca agagctggag gcctttttag cccaccgtga
    49801 ccagattacc gcacttgatt tagccggtga tgaacttggt ttcccgggaa gtctgttcct
    49861 ttctcacttc aaccgcgcgc gtgatgcggg ctggcatatt accgtccatg caggcgaagc
    49921 tgccgggccg gaaagcatct ggcaggcgat tcgtgaactg ggtgcggagc gtattggaca
    49981 tggcgtaaaa gccattgaag atcgggcgct gatggatttt ctcgccgagc aacaaattgg
    50041 tattgaatcc tgtctgacct ccaatattca gaccagcacc gtagcagagc tggctgcaca
    50101 tccgctgaaa acgttccttg agcatggcat tcgtgccagc attaacactg acgatcccgg
    50161 cgtacaggga gtggatatca ttcacgaata taccgttgcc gcgccagctg ctgggttatc
    50221 ccgcgagcaa atccgccagg cacagattaa tggtctggaa atggctttcc tcagcgctga
    50281 ggaaaaacgc gcactgcgag aaaaagtcgc cgcgaagtaa caaaatggat ggtgcaaatt
    50341 gcaccatcca tttttcatgc aaggcacaaa gtcgcgcgat gtttggcgga ttcgatgccc
    50401 agctcaatca actccattac ctggattgcc tggcttgccg gaaccggatt ttcaccatcg
    50461 ccatttaacg catcacgaat agccgcatag taagccggat aattcccagg caccgtcaac
    50521 agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa gtacgccatc acgcatatcg
    50581 tagccccagt cttcctgcgg tagacgctcg ccatttttca gacgttcttc ctgtggatcg
    50641 aggccatatt tcacataact gcctcgggat ccatgcacga tataccgtgc tgactcagcg
    50701 gctgccagca tggtaccgtg taaaatgact cgccgctgtg gataggacaa gatggcgtgg
    50761 aaataatcgg tcgactgcgc tccgggccgt aactgtgcca aatctaccgt catgctgacc
    50821 ggtaaaccaa aaagcgtaat ggcctgatca agaagatgtg gtgctaaatc gtaccagata
    50881 ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca cctgcggacg gaagcggtca
    50941 aaatgagact caaagtaagc aacttcaccc agcacgcctt ccgcgagtaa accttttagc
    51001 gtcaagaaat cgctatccca gcgacggtta tggaatacag acagcacacg ccccaggctt
    51061 tttgccagcg catccagctc tcgcgcttgt gacagtgtca cggtaaaggg tttatcaacg
    51121 accacatgtt tacccgcctc aagcgccgct ttggctaacg ggaaatgggt atcgttgggt
    51181 gtaggaatga caatcaggtc tatgttggga tcgttaaaca gatgcttcgg ctcagagaca
    51241 accgtaaccg ttggccagtc ggcttttact tttgtttcat cactgctgga gattaccgcc
    51301 agttccagcc cgggcgtgcc cgcaatcagg ggcgcatgga aggttttgct cgcataacca
    51361 tacccaatca acccaacacg gatgttgtcg ctcatgtcat tgcctctcat tgaagtatga
    51421 tggctatttg acactatcct ttacccacgc tcaacagttt aataacctgc cagcaataag
    51481 ggatgttgtt taacttaagt caaaaaaata gcgaattttc caacgacaaa agctaaatat
    51541 cgc
//
LOCUS       DAFAVF010000017        48468 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFAVF010000017 DAFAVF010000000
VERSION     DAFAVF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.017"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            153..1370
                     /gene="alaA"
                     /locus_tag="LGK42_000959"
     CDS             153..1370
                     /gene="alaA"
                     /locus_tag="LGK42_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBK2657235.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1454..2053
                     /gene="yfbR"
                     /locus_tag="LGK42_000960"
     CDS             1454..2053
                     /gene="yfbR"
                     /locus_tag="LGK42_000960"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBK2657236.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLESRRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2112..3944)
                     /locus_tag="LGK42_000961"
     CDS             complement(2112..3944)
                     /locus_tag="LGK42_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBK2657237.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4031..4681)
                     /gene="hxpA"
                     /locus_tag="LGK42_000962"
     CDS             complement(4031..4681)
                     /gene="hxpA"
                     /locus_tag="LGK42_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBK2657238.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEYIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4692..5186)
                     /locus_tag="LGK42_000963"
     CDS             complement(4692..5186)
                     /locus_tag="LGK42_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK2657239.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5269..5724)
                     /locus_tag="LGK42_000964"
     CDS             complement(5269..5724)
                     /locus_tag="LGK42_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBK2657240.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6062..7264
                     /gene="ackA"
                     /locus_tag="LGK42_000965"
     CDS             6062..7264
                     /gene="ackA"
                     /locus_tag="LGK42_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK2657241.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7339..9483
                     /gene="pta"
                     /locus_tag="LGK42_000966"
     CDS             7339..9483
                     /gene="pta"
                     /locus_tag="LGK42_000966"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK2657242.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9673..11193
                     /gene="yfcC"
                     /locus_tag="LGK42_000967"
     CDS             9673..11193
                     /gene="yfcC"
                     /locus_tag="LGK42_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK2657243.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGLFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11226..11768)
                     /gene="yfcD"
                     /locus_tag="LGK42_000968"
     CDS             complement(11226..11768)
                     /gene="yfcD"
                     /locus_tag="LGK42_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBK2657244.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11826..12377)
                     /gene="yfcE"
                     /locus_tag="LGK42_000969"
     CDS             complement(11826..12377)
                     /gene="yfcE"
                     /locus_tag="LGK42_000969"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBK2657245.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12433..13077)
                     /gene="yfcF"
                     /locus_tag="LGK42_000970"
     CDS             complement(12433..13077)
                     /gene="yfcF"
                     /locus_tag="LGK42_000970"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBK2657246.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13213..13860
                     /gene="yfcG"
                     /locus_tag="LGK42_000971"
     CDS             13213..13860
                     /gene="yfcG"
                     /locus_tag="LGK42_000971"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBK2657247.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13917..14279
                     /gene="folX"
                     /locus_tag="LGK42_000972"
     CDS             13917..14279
                     /gene="folX"
                     /locus_tag="LGK42_000972"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBK2657248.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14300..15193
                     /locus_tag="LGK42_000973"
     CDS             14300..15193
                     /locus_tag="LGK42_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBK2657249.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIQIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15241..16143)
                     /gene="rpnB"
                     /locus_tag="LGK42_000974"
     CDS             complement(15241..16143)
                     /gene="rpnB"
                     /locus_tag="LGK42_000974"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBK2657250.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="LGK42_000975"
     CDS             complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="LGK42_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBK2657251.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17121..17837)
                     /locus_tag="LGK42_000976"
     CDS             complement(17121..17837)
                     /locus_tag="LGK42_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2657252.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="LGK42_000977"
     CDS             complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="LGK42_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBK2657253.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="LGK42_000978"
     CDS             complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="LGK42_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBK2657254.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19613..20395)
                     /gene="argT"
                     /locus_tag="LGK42_000979"
     CDS             complement(19613..20395)
                     /gene="argT"
                     /locus_tag="LGK42_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBK2657255.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="LGK42_000980"
     CDS             complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="LGK42_000980"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBK2657256.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21325..22842)
                     /gene="purF"
                     /locus_tag="LGK42_000981"
     CDS             complement(21325..22842)
                     /gene="purF"
                     /locus_tag="LGK42_000981"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK2657257.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="LGK42_000982"
     CDS             complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="LGK42_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBK2657258.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="LGK42_000983"
     CDS             complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="LGK42_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBK2657259.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24278..25546)
                     /gene="folC"
                     /locus_tag="LGK42_000984"
     CDS             complement(24278..25546)
                     /gene="folC"
                     /locus_tag="LGK42_000984"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK2657260.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25616..26530)
                     /gene="accD"
                     /locus_tag="LGK42_000985"
     CDS             complement(25616..26530)
                     /gene="accD"
                     /locus_tag="LGK42_000985"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBK2657261.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26686..27345)
                     /locus_tag="LGK42_000986"
     CDS             complement(26686..27345)
                     /locus_tag="LGK42_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2657262.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27428..28240)
                     /gene="truA"
                     /locus_tag="LGK42_000987"
     CDS             complement(27428..28240)
                     /gene="truA"
                     /locus_tag="LGK42_000987"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK2657263.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28240..29253)
                     /locus_tag="LGK42_000988"
     CDS             complement(28240..29253)
                     /locus_tag="LGK42_000988"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK2657264.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="LGK42_000989"
     CDS             complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="LGK42_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBK2657265.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAQLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30554..31549
                     /gene="flk"
                     /locus_tag="LGK42_000990"
     CDS             30554..31549
                     /gene="flk"
                     /locus_tag="LGK42_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBK2657266.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31546..32724)
                     /locus_tag="LGK42_000991"
     CDS             complement(31546..32724)
                     /locus_tag="LGK42_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657267.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIVVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33017..34237)
                     /gene="fabB"
                     /locus_tag="LGK42_000992"
     CDS             complement(33017..34237)
                     /gene="fabB"
                     /locus_tag="LGK42_000992"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBK2657268.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34396..36402
                     /gene="mnmC"
                     /locus_tag="LGK42_000993"
     CDS             34396..36402
                     /gene="mnmC"
                     /locus_tag="LGK42_000993"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBK2657269.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36523..36801)
                     /locus_tag="LGK42_000994"
     CDS             complement(36523..36801)
                     /locus_tag="LGK42_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBK2657270.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36835..37383)
                     /gene="epmC"
                     /locus_tag="LGK42_000995"
     CDS             complement(36835..37383)
                     /gene="epmC"
                     /locus_tag="LGK42_000995"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBK2657271.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37383..38192)
                     /locus_tag="LGK42_000996"
     CDS             complement(37383..38192)
                     /locus_tag="LGK42_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK2657272.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38192..39016)
                     /gene="mepA"
                     /locus_tag="LGK42_000997"
     CDS             complement(38192..39016)
                     /gene="mepA"
                     /locus_tag="LGK42_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBK2657273.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39020..40105)
                     /gene="aroC"
                     /locus_tag="LGK42_000998"
     CDS             complement(39020..40105)
                     /gene="aroC"
                     /locus_tag="LGK42_000998"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK2657274.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40140..41072)
                     /gene="prmB"
                     /locus_tag="LGK42_000999"
     CDS             complement(40140..41072)
                     /gene="prmB"
                     /locus_tag="LGK42_000999"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK2657275.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41238..41789
                     /gene="smrB"
                     /locus_tag="LGK42_001000"
     CDS             41238..41789
                     /gene="smrB"
                     /locus_tag="LGK42_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBK2657276.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41862..42713)
                     /locus_tag="LGK42_001001"
     CDS             complement(41862..42713)
                     /locus_tag="LGK42_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBK2657277.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42715..43254)
                     /locus_tag="LGK42_001002"
     CDS             complement(42715..43254)
                     /locus_tag="LGK42_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657278.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43251..43739)
                     /locus_tag="LGK42_001003"
     CDS             complement(43251..43739)
                     /locus_tag="LGK42_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657279.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43736..44245)
                     /locus_tag="LGK42_001004"
     CDS             complement(43736..44245)
                     /locus_tag="LGK42_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657280.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44261..45013)
                     /locus_tag="LGK42_001005"
     CDS             complement(44261..45013)
                     /locus_tag="LGK42_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2657281.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45033..47675)
                     /gene="yfcU"
                     /locus_tag="LGK42_001006"
     CDS             complement(45033..47675)
                     /gene="yfcU"
                     /locus_tag="LGK42_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBK2657282.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47757..48320)
                     /locus_tag="LGK42_001007"
     CDS             complement(47757..48320)
                     /locus_tag="LGK42_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2657283.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11813 a        13146 c        12140 g        11369 t
ORIGIN      
        1 tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat cttattctgc
       61 agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg
      121 agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat
      181 tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag
      241 aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc ggttttgacg
      301 cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg
      361 attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca
      421 tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc
      481 aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca ccagattacc
      541 cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg
      601 aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc
      661 gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt
      721 taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc gatgaaattt
      781 atgacaaaat tctttacgac gacgctgagc atcactcaat tgcgccgctg gcacctgacc
      841 tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg
      901 ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa
      961 tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc
     1021 tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc
     1081 gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg
     1141 gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac gacgatcaga
     1201 aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca
     1261 actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc
     1321 tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc
     1381 actgccggag attgcatccg gcagcgttat cccgccacaa tgacctgatg atgtcatcat
     1441 acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc tgaaactcat
     1501 taaccgctgg ccgctaatgc gcaacgtgct gacggaaaat gtgtccgaac acagtttgca
     1561 ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cgaaaatttg gcggtaatgt
     1621 caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag tgctcaccgg
     1681 cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt acaaggctat
     1741 tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc gggatatctt
     1801 tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc
     1861 agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt
     1921 cttgctggca aaaacgcgac tggaagcgac gcttgaatcg cgtcgcagcc aggagatgga
     1981 ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga
     2041 ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca
     2101 tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcaca
     2161 ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt tcccggacct
     2221 aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga tgctgccatc
     2281 gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc agccagcgca
     2341 atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat aaccgccgac
     2401 aagacaaaca aacaacccag catcatatgt ggcccgtaac caccgccaat gtccatcagc
     2461 cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc
     2521 ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc agcatctata
     2581 cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca
     2641 tctgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg
     2701 tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc
     2761 tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat atcgcccagc
     2821 agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt tagccccacc
     2881 acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aatcagttct
     2941 gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac
     3001 tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca
     3061 tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta
     3121 acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcgcca
     3181 taacgctcac gtaatttgag atcatccagc cgttgaccaa tcattggcga tccggggcga
     3241 atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt
     3301 cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc
     3361 actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag
     3421 aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag gttcggcggc
     3481 gtcgccacca gcgtcatcat gccgcttatc agcccggcaa aactcaacgg catcatcaga
     3541 cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat aaagatagcg
     3601 acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat caacaaaacc
     3661 aacattttga tttcactgtt gcccgcaact ttgaccagcc atgttcccat tacggtggca
     3721 acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag gacaacgtta
     3781 ggatcagaaa agccggaaaa tacttcgggg actgtcagcg ttccgcttaa tgcaaacgcg
     3841 acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacaatg
     3901 gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa
     3961 ttgagggaga tgtcttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg
     4021 atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct
     4081 ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa
     4141 ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga gcatcttcca
     4201 ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc gcctaacaga tacgcatcag
     4261 gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc
     4321 cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg
     4381 gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc gcggtaatac
     4441 cttcggtttc cgtggcctcg atgtactcca gacgcgtaaa ttcggcggca atatcagcct
     4501 cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag
     4561 ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta
     4621 ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca
     4681 cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga
     4741 cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg
     4801 gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac attaaccata
     4861 aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca
     4921 cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga
     4981 tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg
     5041 cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg
     5101 taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata
     5161 agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctact acggtgaaaa
     5221 aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc
     5281 aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta
     5341 atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac
     5401 ttcataaaac cagttgagga tagcaggggg taatggcgtt acagaacgct tgcccagcca
     5461 ccataatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag
     5521 ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg
     5581 gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt
     5641 ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac
     5701 agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat
     5761 ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact
     5821 atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat
     5881 tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc
     5941 ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca
     6001 tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc
     6061 catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat
     6121 catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc
     6181 cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg
     6241 cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga
     6301 actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac
     6361 cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc
     6421 accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca
     6481 gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga
     6541 gtcttacctc tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg
     6601 cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc
     6661 ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat
     6721 ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat
     6781 gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg
     6841 catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac
     6901 cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa
     6961 gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct
     7021 gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat
     7081 ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg
     7141 caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc
     7201 ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc
     7261 ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt
     7321 aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc
     7381 ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg
     7441 agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg
     7501 actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac
     7561 gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac
     7621 taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt
     7681 aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc
     7741 gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg
     7801 acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa
     7861 ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac
     7921 gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg
     7981 ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat
     8041 atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt
     8101 aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt
     8161 tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc
     8221 atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc
     8281 atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc
     8341 aacacctggc agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat
     8401 cacgaacgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc
     8461 gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat
     8521 cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa
     8581 ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg
     8641 ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca
     8701 gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa
     8761 ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg
     8821 gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt
     8881 cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc
     8941 tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac
     9001 tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct
     9061 gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt
     9121 acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa
     9181 aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac
     9241 gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc
     9301 ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc
     9361 tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca
     9421 ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag
     9481 taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat
     9541 ttccggtttt ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg
     9601 ctgcatcaat gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg
     9661 aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc
     9721 gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt
     9781 ccggtggggt tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca
     9841 cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag
     9901 tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg
     9961 tttgaaggat taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg
    10021 ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc
    10081 ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt
    10141 ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt
    10201 atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc
    10261 tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt
    10321 gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg
    10381 gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag
    10441 aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat
    10501 gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta
    10561 atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc
    10621 cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc
    10681 atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct
    10741 gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcaggt
    10801 gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac
    10861 aacgctgtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt ctttgtgaca
    10921 tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc
    10981 ggtgttaacc gtcaggttac cgtgctggca ttccagtttg gtgatggctt cagccacatc
    11041 atttacccaa cctcagcttc gttaatggcg acgctcggtg tttgccgggt ggacttccgt
    11101 aactggctga aggtgggcgc gacactgctt ggactgctgt ttattatgtc cagcgtcgtg
    11161 gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc
    11221 ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattacg cttcatccac
    11281 aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc
    11341 ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag
    11401 acgcagctga acaatgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg
    11461 tgctcagcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat
    11521 tccagcagtt gctcatcggc ctggaccaca ccgcctgcgg tcgcatctaa catgccgggt
    11581 aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg
    11641 acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt
    11701 gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc
    11761 tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg
    11821 gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca
    11881 ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt cggaatactc
    11941 accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta
    12001 tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga
    12061 tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt
    12121 atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata
    12181 accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt
    12241 aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg
    12301 ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat
    12361 gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt
    12421 ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc
    12481 tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag
    12541 taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa
    12601 attcggctga cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc
    12661 ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg
    12721 ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc
    12781 acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc
    12841 cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg
    12901 cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag
    12961 gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa
    13021 cacataaggg gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat
    13081 gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt
    13141 gacgaatact cacgtaagtc gccgcgcttt ttatacttgt ggtgttcaga tgattaaaca
    13201 accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg
    13261 ctgtttctcg aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc
    13321 ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt
    13381 gatcattctc ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg tgccattttg
    13441 ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc
    13501 acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat
    13561 cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa
    13621 actcagcgtc tttaccatgt actgaacaag cggctggaaa actcgccctg gctgggaggc
    13681 gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag
    13741 cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc
    13801 cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa
    13861 cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg
    13921 cacaacctgc cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta
    13981 aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg
    14041 ccgataaagc gcgtaccagt gaagatatta atgacgcgct gaactatcgc accgtcacca
    14101 aaaacatcat tcaacatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg
    14161 atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata
    14221 aactacacgc gctgcgctac gccgattcgg tatccatgac cttgagctgg cagcgttaac
    14281 cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg
    14341 ccatttgatt ccgcgtttgc tggagttggg ccatcaaatc acggtagtga cgcgtaaccc
    14401 gcagaaagcc agttccgttc tcggccctcg ggtgacacta tggcaagggc tagccgatca
    14461 aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa
    14521 acgctggact cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa
    14581 actggtcgat ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc
    14641 aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa
    14701 cgaatttact cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga
    14761 caaaacgcga gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct
    14821 cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca
    14881 gtatcttgcc tggattcaaa tcgatgatat ggtcaacggc attctctggc tgctggataa
    14941 cgagctgcgc gggccattta atatggtttc gccctacccg atacgcaatg aacaatttgc
    15001 ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg cgcgtccccg caaccgccat
    15061 tcggttgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa
    15121 acggctggaa gaagcgggtt ttgcgtttcg ttggtacgat ttagaagagg cgctggcgga
    15181 tgtcgttcgc tgatgtggtt tactgcaaac atccgccagt taactcccgg tgttacaggg
    15241 ttagtggctt tgcgctataa gatcgtctgg cgatagtcgg gtcaccatca taactaactc
    15301 tctgtctaaa cctctatcca gcatctcctg agcaatacgc agggcttctt cgcgtttgcc
    15361 cagaattaat ccatcattcc gcccttcttc acgtaatctg tcagcaatgg tcatcagttt
    15421 ctccttttct tgtggtgcgc gttctgctat ctcaccaata aatgcacgga aacgctgggc
    15481 atctcccgtt tgtaatacat aattaaacag ggcttttagc tgtctgtcat tagtgttccc
    15541 tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct gacgaatatg
    15601 tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt catccggcac
    15661 cacggtaata tccaccaacg gaaaagccga tgaatatatt ttacgggcaa tcgcaggctc
    15721 agcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac catgataaaa
    15781 cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt tttgcattgc
    15841 cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac tttggtgctc
    15901 tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca agaggtcgga
    15961 ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa gcgtcgttaa
    16021 atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc
    16081 tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg tcgatattgt
    16141 catggcgttc cgtcacccat caaatttaag atgacgcgac aataaccacg attaacaccc
    16201 aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt agcggactgc
    16261 aacttttaca tttcagcgga agctgactcg caaattcccc atgaaaatac tccggcagcg
    16321 ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc agacgagggc
    16381 tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttc ccctgatgga
    16441 ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc actaccacca
    16501 tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct accagttcag
    16561 gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc gccagcgccc
    16621 gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat ttcccctgcg
    16681 cacgttcgtc tatcccgact tttgccagat acttcaccgc ccgctcgcgc gcttcctgct
    16741 tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc gtcatatggc
    16801 tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat
    16861 ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc gtctggccat
    16921 tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac
    16981 ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc gcttgcagtg
    17041 agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata acgtttaatt
    17101 tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt
    17161 ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata gagcaccgcg
    17221 gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg ggctattttc
    17281 agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat caggatcact
    17341 tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat gcaacgatac
    17401 attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg cggaaccgaa
    17461 cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc
    17521 acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc cttaacaatc
    17581 tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg cgtaccacga
    17641 aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga gacacgacca
    17701 atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag cagccacaga
    17761 gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt ccagtattca
    17821 tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg cgctcaagga
    17881 acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca cagacgatgg
    17941 cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg accacatctt
    18001 ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag ttgttgccaa
    18061 tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac acttgcccac
    18121 gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc ataaacgcgc
    18181 cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata ataccagcga
    18241 ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa
    18301 tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct cgaatcagcg
    18361 tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta ccgccagcgc
    18421 caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc agcgtgacga
    18481 gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg
    18541 tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc tttctttcac
    18601 cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta atttctcgta
    18661 agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc
    18721 ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt taacagacgg
    18781 gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc tggcagcgac
    18841 ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg
    18901 cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac cgaacgtctc
    18961 ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga ctgtcggctg
    19021 aatgtcggaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa
    19081 ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt
    19141 cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg tacattgcgt
    19201 attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca gttcgccttg
    19261 tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg
    19321 aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga gcgatagcac
    19381 cagttttttc ataatgtcct caactgacag tctttttata gggaatattt acaggtttgt
    19441 tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc gttagacgta
    19501 ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag tgcaacaaac
    19561 ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg tatcagtcac
    19621 cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg tcctgacgca
    19681 gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta cgtagcccta
    19741 caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca aaggcgaaat
    19801 ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct tgtaacgcag
    19861 catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca taggccacca
    19921 catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt gatccctgca
    19981 gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg
    20041 cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac
    20101 gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg
    20161 ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc cgtttgcaca
    20221 tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct ttcgacgaga
    20281 acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc gcatagctgg
    20341 aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc ttcatacata
    20401 accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt
    20461 tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa atatgttaat
    20521 aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg aatcgcagcg
    20581 tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca aaattgccct
    20641 gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc ttcaggaagg
    20701 gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc atcaagggat
    20761 tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat ttctgccgcc
    20821 tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca gagcaccaac
    20881 gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc agtatagcta
    20941 tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg
    21001 aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat
    21061 gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg cgccgcctgg
    21121 ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa taagcgcacg
    21181 ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat aaatagttgc
    21241 cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag tcagggtgcc
    21301 agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat tttccacttc
    21361 gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga aatcgaggta
    21421 gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt caaactgctg
    21481 gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa
    21541 cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc
    21601 cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg gtgccgcaga
    21661 agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct gctcagaagt
    21721 ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga actcggcgcg
    21781 gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg gcatgataaa
    21841 ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc ccagaatacg
    21901 agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca cgtcgatatc
    21961 cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat tcacacgcgc
    22021 gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata catactcaaa
    22081 caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact gcccttcttc
    22141 agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc
    22201 gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt
    22261 tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat
    22321 aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag caatggcagc
    22381 gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc tagcgaagat
    22441 attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt cttcaaacag
    22501 ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg taatgccata
    22561 cggggagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag ccgtagggta
    22621 acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag cttcaaatac
    22681 atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat ctatggtgat
    22741 gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg
    22801 gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt ttcctcgtta
    22861 agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt gcagataatc
    22921 aaaaaaccat ctgatgataa aactgaactg cgggatcagc tgtgatttgc tccagtcttc
    22981 gcttttcgac accccggtaa aggagtcgag aaagaataga atggcggcaa caatcaacac
    23041 accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca accccgtttt
    23101 ctccaccagc tggcctatca cgaagttcac gatagcacca acgatcaggg tagcgataaa
    23161 tagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg taaaccagac
    23221 tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg tcaccagcga
    23281 taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag caatcaccgc
    23341 gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc tgtcatcctg
    23401 ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat
    23461 caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc tttgcggcat
    23521 cgtgcaatgc gtgtcggatg cggcgtaaac gcctgatccg acctacggtt ctaaccctgc
    23581 ccagtcctga taagacgcgc cagcgtcgca tcaggcaagg ccgtattaat tcggcgtata
    23641 gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg aacctttcag
    23701 cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct gcactggcgt
    23761 ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa tctcatttac
    23821 tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg ttggcgctgc
    23881 tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt caaccttcgg
    23941 tttaggcggc tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa
    24001 ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac ggtgctgcgg catcacctgc
    24061 ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg
    24121 catcatatca ggctcatcac gatcgcccgc tttcggcacc agcgggatag ccgcgaactc
    24181 atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc
    24241 caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg
    24301 cgtcaatcac ttccatgaca tgtgcgaccg tgtggaaaga accacacacc agcacggtgt
    24361 cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta tcaaatgatt
    24421 tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg
    24481 gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt
    24541 tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc gctttcatac
    24601 gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg
    24661 actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg
    24721 cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgctgtt gcggcattcg
    24781 gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc
    24841 agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacctt
    24901 tttcctgcgc cacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt
    24961 ttgcgctgcg aaagatgcct gctttctcgc gaccaatact ttcgcgatct ggtcccagcc
    25021 agtcggtatg atccagcgca atgctggtta ctacagccac atcggcatcg acaatattgg
    25081 ttgcgtccag acgaccgccc agccctactt ccagaatcac tacgtcaagt tgtgcctgtt
    25141 tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc
    25201 gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat tcctgcccct
    25261 gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg cccactttgt
    25321 accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg ccattcgtac
    25381 ccgcaacggt aaacacaaat ggcgctggtt tcaggacgcc aagacgcgcc gcgacctggc
    25441 tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc
    25501 acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt
    25561 cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg
    25621 cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc gcttcaggat
    25681 tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg
    25741 gacgacggac gatcatgtcg atggcgcctt tctcgatcag gaattcactg cgctggaatc
    25801 ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca aagccaatta
    25861 acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc
    25921 ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca
    25981 gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc
    26041 caccagaggc ggagaagcag atcagcgggc agttatcttc cagcgcctgc tcaacggcac
    26101 gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg aactcgaatg
    26161 ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc gcatcttttt
    26221 cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact
    26281 tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca
    26341 ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct
    26401 caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc
    26461 acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa
    26521 tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg
    26581 acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag
    26641 cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg
    26701 tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg aaacaaaaat
    26761 aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc cgaagaaata
    26821 acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat aagcggcgaa
    26881 atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg
    26941 tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt
    27001 atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt tgtcaccaaa
    27061 taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca acataagcac
    27121 caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac cagcaacaaa
    27181 cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc agaacagaat
    27241 caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg ccagatgcac
    27301 gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg cctatgcctt
    27361 gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt
    27421 attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcataccggt
    27481 cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgccagcg
    27541 ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga
    27601 cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga
    27661 tatctaccac cacataagga ccgtgacgcg tgacattaat atgcataacg ttgcgccacg
    27721 gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc
    27781 attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt
    27841 tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa cggcgagccg
    27901 tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt
    27961 caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc tgcgcggtcg
    28021 tttcgaaatg cactacctgc ccggtaccgt gtacaccagc gtcagtacgc ccggcgcaga
    28081 agacggtaat gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac
    28141 tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac tcaatgccca
    28201 gcgcaatttt ataaactggc ggttgttgct ggtcggacat tagtacagat actcctgcac
    28261 cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac
    28321 cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc caacagaaag
    28381 atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt cagagagcac
    28441 aatatcttcg ccctgagcaa acgcatcacg cgcttcttct gccgccagcg gacgcagtgc
    28501 ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga
    28561 aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc
    28621 acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca gttgacgacc
    28681 gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct gccccgctaa
    28741 cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cactgatacg
    28801 cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc tggtcagact
    28861 gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc
    28921 cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt cgatcaccag
    28981 gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa
    29041 aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg tgattgtctt
    29101 accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc
    29161 aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc
    29221 gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc
    29281 tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg
    29341 gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac
    29401 agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt
    29461 atcccggcga ctttacgcag ctgtgcgtca tcgcggcgca catcatacac caaatgcacc
    29521 agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc aaactctggc
    29581 gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt gctataggct
    29641 tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc gatatgcggc
    29701 gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc ttcccagaca
    29761 tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc agtattatcg
    29821 acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct acggatcagt
    29881 ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa
    29941 gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc ctcatcccca
    30001 cggtcagcgc gaggcggatc gcaaagcaag gttttaatcc ctaacgcttc cagtcgcgct
    30061 tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc gtgcagtgaa
    30121 aatccatcgc gttcggcaag catcagcagg gaggagaaaa catattccac caccgcaatc
    30181 gcattacagc caggtgccgc ggaaaaacca attcccgcct gctttaacca tgcttcatcg
    30241 acatggtcgg tccctgctgt ggcagtacca acaaatttaa tgggttttcc tgccagcaaa
    30301 gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg
    30361 acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa taagtcgcgg
    30421 gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag
    30481 agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt
    30541 ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac caccaggtca
    30601 gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc
    30661 gctggaaagc ttaatgacga aagtgacctc actgacgcaa cagcaaagag cagaactgtg
    30721 ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt
    30781 ccctgccgct gaacataatc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc
    30841 ccgccagctt ttagcgcaat taggcgagta tttacgtctg gggaataatc gtcaggcggt
    30901 cacggattat atccgtcata actttggtca gacgccgctg aatcagctct caccggagca
    30961 attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc aaccgcagca
    31021 gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctca aacagctggt
    31081 gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat cgatgctgga
    31141 actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac
    31201 ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg aatcactaca
    31261 gatggcgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg catatatcca
    31321 gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct
    31381 taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct
    31441 taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg
    31501 gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc cccgacgaaa
    31561 tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga
    31621 agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc cgagggcgat
    31681 atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt
    31741 aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc
    31801 gcccgctaat gcgacccacg cacctggggc ttgccagagc aacaacaagc ccaccgtttc
    31861 tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa
    31921 catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc
    31981 ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc
    32041 ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc acagcgatgg
    32101 acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc acgccagtaa
    32161 gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat gaatcaacag
    32221 gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc atgacatcac
    32281 tttgccagag tgttttgccc ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa
    32341 ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa atttgaacgg
    32401 tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac cacaagctaa
    32461 catcccctga agcgccgaac gttttgcgcc atattgatcg gccagtcgcc cggcatagcc
    32521 acgcgtcagc accgtagcca gaaactgaat cccgacgaca atcccgacca tggtattgcc
    32581 atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc ctacggtcat
    32641 gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt
    32701 ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat
    32761 ccccgtctca tctctctggt ttcagggtta cagtgcgttg gcaggattta acgcgtacgt
    32821 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    32881 gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc cgcattagcg
    32941 cgtaacgtag gatgcgacgc tggcgcgtct tatccgacct actccgacta ctccgactac
    33001 tccggcctac tgcgaattaa tctttcagct tgcgcattac cagcgtggcg ttggtgccgc
    33061 cgaagccgaa gctgttagac ataacggtgg tcagttcgcg atcggtcgtt tcggtcacga
    33121 tgttcagacc tgcagcctgc tcgtccagct cttcaatgtt gatgctcggg gcgataaagc
    33181 cgtgttccag catcagcaga gagtagatag cttcctgtac gccagcagcg cccagagagt
    33241 gaccggtcat ggctttggtt gcagaaatcg ccgggctctt atcgccgaac acttcacgga
    33301 tagctgccag ttctttcacg tcgccaaccg gagtcgaagt accatgagag ttcaggtaat
    33361 cgattggggt atcaacgccg tgcatcgcca tcttcatgca gcgtactgcg ccttcgccag
    33421 acggagcaac catgtctgca ccatcagagg ttgcgccgta gccaacgatt tcagcataga
    33481 tgtgagcacc acgcgccagc gcgtgttcca gctcttcaac cactaccata ccgccgccgc
    33541 cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt
    33601 cgttgtattt agtagacagc gcacccatcg cgtcgaattc gcaggccatt tcccagcaca
    33661 gctcttcgcc gccgccagca aacacgatgt cctgtttgcc cagttggatc tgctctactg
    33721 cgttaccgat acagtgtgcg gaagtcgcac acgcggagct gatggagtag ttaacgccat
    33781 gaattttaaa cggcgtggcg aggcaggcag aaacgccgga tgccatcgct ttggtgacca
    33841 catacgggcc aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg ccgaacacct
    33901 ggaaacgcgg ggaaccgccg ccagaacctg caatcaggcc aacgcgcggg ttattctggt
    33961 aagcttccgg agaaaggccc gcatctgcga ttgcctgctc catagaaagg aatgcataaa
    34021 tggatgcgtc gctcataaag cgcacaactt tgcggtcaat gaggccagtg gtatccagtt
    34081 ttacgttgcc ccagacgtgg ctacgcatgc cggaatcctt cagctcctga gagaaagtga
    34141 tccctgaacg tccttcacgc agagatgcca ggacttcctg ctggttatta ccgatgctgg
    34201 aaacaatgcc caggccagta atcactgcac gtttcattca atacctctgt aagtcgcaca
    34261 tagagtaagt ttcgaatgca caatagcgta cacttgtacg ccgaacaagt ccgatcagcc
    34321 atttaataga gaaatttgcg cagccttaca cacatcgcta agatcgagcc accgcctgta
    34381 agacgagtaa cttacgtgaa acactactcc atacaacctg ccaacctcga atttaatgct
    34441 gagggtacac ctgtttcccg agattttgac gatgtctatt tttccaacga taacgggctg
    34501 gaagagacgc gttatgtttt tctgggaggc aaccaattag aggtacgctt tcctgagcat
    34561 ccacatcctc tgtttgtggt agcagagagc ggcttcggca ccggattaaa cttcctgacg
    34621 ctatggcagg catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta
    34681 catttcatta gttttgagaa atttcccctc acccgtgcgg atttagcctt agcgcatcaa
    34741 cactggccgg aactggctcc gtgggcagaa caacttcagg cgcagtggcc aatgcccttg
    34801 cccggttgcc atcgtttatt gctcgatgaa ggccgcgtga cgctggattt atggtttggc
    34861 gatattaacg aactgaccag ccaactggac gattcgctaa atcaaaaagt agatgcctgg
    34921 tttctggacg gctttgcgcc agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac
    34981 gccatggcaa ggttggcgcg tccgggcggc acgctggcga catttacgtc tgccggtttt
    35041 gtccgccgcg gtttgcagga cgccggattc acgatgcaaa aacgtaaggg ctttgggcgc
    35101 aaacgggaaa tgctttgcgg ggtgatggaa cagacattac cgctcccctg ctccgcgccg
    35161 tggtttaacc gcacgggcag cagcaaacgg gaagcggcga ttatcggcgg tggtattgcc
    35221 agcgcgttgt tgtcgctggc gctattacgg cgcggctggc aggtaacgct ttattgcgcg
    35281 gatgaggccc ccgcactggg tgcttccggc aatcgccagg gggcgctgta tccgttatta
    35341 agcaaacacg atgaggcgct aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg
    35401 ttttacgacc aattacccgt taaatttgat catgactggt gcggcgtcac gcagttaggc
    35461 tgggatgaga aaagccagca taaaatcgca cagatgttgt caatggattt acccgcagaa
    35521 ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc
    35581 agcggcatta cttatccgca aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg
    35641 ctggaactgg cgcaacagca gggtttgcag atttattatc aatatcagtt acagaattta
    35701 tcccgtaagg atgactgttg gttgttgaat tttgcaggag atcagcaagc aacacacagc
    35761 gtagtggtac tggcgaacgg gcatcaaatc agccgattca gccaaacgtc gactctcccg
    35821 gtgtattcgg ttgccgggca ggtcagccat attccgacaa cgccggaact ggcagagctg
    35881 aagcaggtgc tgtgctatga cggttatctc acgccacaaa atccggcgaa tcaacatcat
    35941 tgtattggtg ccagttatca tcgcggcagc gaagatacgg cgtacagtga ggacgatcag
    36001 cagcagaatc gccagcggtt gattgattgt ttcccgcagg cacagtgggc aaaagaggtt
    36061 gatgtcagtg ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca
    36121 atggtaggca atgttcccga ttatgaggca acactcgtgg aatatgcgtc gttggcggag
    36181 cagaaagatg aggcggtaag cgcgccggtt tttgacgatc tctttatgtt tgcggcttta
    36241 ggttctcgcg gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc gcagatgagc
    36301 gacgaaccga ttccgatgga tgccagtacg ctggcggcgt taaacccgaa tcggttatgg
    36361 gtgcggaaat tgttgaaggg taaagcggtt aaggcggggt aatctgctct cgcagtgttt
    36421 gccggatgcg gcgtgaacgc cttatccgac ctacgtgtga cgtgtaggcc tgataagacg
    36481 cgataagcgt cgcatcaggc atggtgctcc agacgccgca acttactgct gtgacgcctg
    36541 ctgaaacagg ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg acaattcacc
    36601 ggcaccaatg gctttttcca gactctggct aacggtcgta tgcaccgcct gagcggagtg
    36661 gtcatcacca ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact
    36721 ggcaaacaac tcatcatcac tggcatggtc aaccataccg tcgattaatg ccagaatgcg
    36781 tgattcaaac tccgcgatca tcttctttcc tcattgtaaa acgtggcgct ggctttagtt
    36841 gagcgcttcc ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg
    36901 tagtgcttta atgaagcgtg ccgggcgttc aggaatgccg tttgctaggt aatccatcac
    36961 ctgcgcatgt acccgccgtt gaaacaccac acgatccggt tcgaagtcgc cttccagatt
    37021 gtcgcaactg acgttaaacg gatatcccgc cgccacacag aacaaccagt cgagtgcctg
    37081 cggcttcact tcaacatctt caaactggct ttgcgtttgg gcatcacgcc catccgggca
    37141 ataccagtag ccgaaatcaa ccagctcacg gcgcgctttc ccggcaatac accagtgcga
    37201 aatctcatga attgcgctgg cataaaagcc atgagcaaag acgattcggt tatacggtac
    37261 ttccgcatca gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa
    37321 ttcatcggca aagcagctat taaaaatttc aatcaactgc tcatagtggt gtgtactgtt
    37381 cattagttca tccccaacca gtggaggatc tcctgtccgt ggctatcaaa aagtaatttg
    37441 gcactcatca ccgctgagac aataacaatc atcgggcgga tcagcttttg tcctttgctc
    37501 aacaccagtc gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc
    37561 gtcgcccaaa tcactttgcc accgagaata aacagcagta aaccgccgat gtttgacgtt
    37621 gcgttgagta atttggcgtg cgccgtggct ttggcgaggt tgaatccgca cagcgtaaca
    37681 aaggccagtg cgtaaaacga tccggctgcc ggaccaaaga atccgtcata aaaaccgacg
    37741 cagccaccgg caattaacgc gaagggtaaa ccgtacatcc gacgctggcg atcttcttca
    37801 cccagctttg gcatcagcaa gaaatagagg ccgatacaaa ttaccagaat gggcaaaatc
    37861 tgccgtaaga catcagcctg aacgtattgc accagcagtg cgccgctcat tgagccgaca
    37921 aaggtcatgg cgatattgag tttctgatcg cttaaactaa ccactttgcg gcgaataaag
    37981 tagatagtgg cggaaataga gccgccgcag gcttgcagtt tattggttgc cagcgcgtta
    38041 gcgggagaca ttcccgcagc catcaatgcc ggaatggtga gtaacccacc gccaccggca
    38101 atcgagtcga taaatcccgc cagcatggcg acaaaaaaga gaactcccag caacagcggg
    38161 gaaaccataa acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct
    38221 ggcaggaagg cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cccggttttg
    38281 gaggttcaaa ccagctttgc agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt
    38341 gatcttcaca ctccagacta tcggcaggac aacgtaatcg tacatgcata tgcgcgcgat
    38401 gctggaacca gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa
    38461 gttgttgttt aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga
    38521 gtttgatcaa gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc
    38581 gggaaactaa gtccagtgct tgcgggcgca agagctgcgc ggaggtccag cgagttttcg
    38641 gcagttgcag aaagatatcg acatccagtc cggtctggtg gctggcatga ccgccgttga
    38701 aacgcccacc agcaggcatc cccatatcgc caatcagcac cgtacccatg cccagattgc
    38761 tcacctggct actcagacgc tggataaaca tcaccaggtc cgggtgaccg aaatagcgac
    38821 gctgatcggt acgcatgacc tgataatgtt cggactgtat cggcagcgtg tcagcgccga
    38881 cgatacagcc attagaaaaa ctgcctatcg attgtgcgct acccggcaca ggttgggtta
    38941 ttttttgcca cggcgtcgct gccaggctag cgctactggc aagcagagcc agcagcgcaa
    39001 tcgcggtttt gttcattttt taccagcgtg gaatatcagt cttcacatcg gcattttgcg
    39061 cccgctgccg tagcaggtga tccattaaaa cgatcgccag catcgcttca gcgatcggca
    39121 ccgcgcggat cccgacacag ggatcgtgac ggcctttggt gatcatctca acttcttcgc
    39181 caaagcggtt aatagtgcgc cccggcacgg taatgctgga ggtcggtttc agcgccatat
    39241 gggcaatgat ttgctgcccg ctgctgatac cgccgagaat gccgcccgca tggttgctct
    39301 ggaaaccgtc tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa
    39361 aaccgtcgcc aatttccacg cctttcaccg cgttgatgct catcagcgca tgggcgatgt
    39421 cggcatccag gcggtcaaag accggctcgc caagtccggc ggggacacca ctggcaacaa
    39481 cggtgacttt cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc agttcatcta
    39541 acgcgtcgat tttgtccggg tccgggcaga agaacggatt ttgttcaacc agcgaccagt
    39601 ctttgatttc cagcggaatg tctcccatct gggtcaggca gccgcggatt tcaataccaa
    39661 atttctcggc gagatatttt ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc
    39721 gggcggaaga acgtccaccg ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt
    39781 aatcggcatg gcctggacgg aaaacgtcct taatcgcact ataatcctga gaacgctggt
    39841 cggtattttc gatcaacaag ccaatgctgg tgccggtagt aacgccttca aaaacaccgg
    39901 agagaatttt gacctgatcc ggctcgcggc gctgggtggt atagcgcgat gtcccagggc
    39961 gacgacggtc gaggtcatgt tgcaggtccg cttccgtcag cggaatgcct ggcggaacac
    40021 catcgacgat gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt acgcgaaaga
    40081 gttgtccaat tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact
    40141 taatctttat aaatcgcgaa atattcacgg gccgccagca gctgctcttt ggtgagcata
    40201 aacacaccat cgccgccgtt atcaaactcc agccaggtga acggaacatc cggatattgt
    40261 tccataagat gtaccatgct gttgccgact tcacaaatca gcacaccatc atcagcaagg
    40321 taatctgccg cgttaccgag aatgcgacgc gtcagtttca ggccgtcggt gccagatgcc
    40381 aggcccagtt ccggctcgtg gcggtattca tttggcaggt cggacatatc ttccgcatcg
    40441 acatacggcg ggttagtgac aatcagatcg tactgcactt tcggcaagtc gcggaacaga
    40501 tcggaacgaa tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcggca
    40561 accgccagcg cgtcgggaga gatatctacc gcgtcgactt ccgcttccgg gaaggcataa
    40621 gcacaggcaa tggcgatgca gccactaccg gtacacatat ctaaaatatg ctgcggttgc
    40681 ttgctgataa gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc
    40741 agcacgcgtt catcgacgta aaattcatgg ccacagaacc aggctttgtt ggtcaggtag
    40801 gccaccggaa tgcgctcgtt gacgcggcga atcacgcgct caacaatacg gtgtttttcg
    40861 ctggaggtca gacgcgcggt gcgcatgtct tccgggatat ccaacggcag gtagagcgaa
    40921 ggcaacacca gttgcacggc ttcatcccac gggttgtcag tgccgtggcc gtaccagata
    40981 ttcgccgcgc tgaagcggct caccgaccag cgcaacatgt cctgaatggt ttgcagctca
    41041 tttactgctt catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa
    41101 tttcggcggc tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga
    41161 ggaaaaatac gtttaaaacg atcgattgcg ctacgagtcg ggtaaactgt aggaaaatta
    41221 gaaatagaga cagataaatg aaaaagaaaa caacacttag cgaggaggac caggctctgt
    41281 ttcgccagtt gatggcgggg actcgcaaga ttaagcagga cacgattgtc caccgaccgc
    41341 agcgtaaaaa agtcagcgaa gtgccggtga agcggttgat tcaggagcag gctgatgcca
    41401 gccattattt ctccgatgag tttcagccgt tactgaatac cgaaggtccg gtcaaatatg
    41461 ttcgcccgga tgtcagccat tttgaggcga agaaactgcg ccgtggcgat tattcgccgg
    41521 agttgttttt ggatttgcac ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt
    41581 tgattgccgc ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga
    41641 agcatatttt gaagcaacaa acaccgctgt ggctggcaca acatccgcat gtgatggcat
    41701 ttcaccaggc accgaaagag tacggcggtg atgcggcttt gttggtgttg attgaagtgg
    41761 aagagtggtt gccgccggag ttgccctgag gagttgagcg tgttgtgcca ggagcgcgga
    41821 atggcagggg cgaatgaccc tgccaggtga gaaaaggtgc gctaatacat attcagtatg
    41881 taagtgaatc ggcaggtaga atctatgggc tgtcgagatg tcgtgtccac gcgtagcttc
    41941 atatcatgca gttgcctgga cgtgatcggt tgcggctctg gcgtcaccgt cactttggca
    42001 ttatcacagc cggaagtcga gaaggtcatc tgctgtgcga caaaacggca gtaggctcca
    42061 cgctcgtcaa aattactgcc atcacacagt ttgccgtttt ccatcaggaa agtgggcagt
    42121 gtttgctcaa taacaccacc tgttgactgc aacctgacga ctttgttatg tacaacataa
    42181 ttttcattcc aggaaacatc atacggtgcc agggactctg tcgggcagtc agagctaact
    42241 tttgttggtc ctataaaaat tccggccccg aaacgctgat ccatagtact ggcgctttca
    42301 acataagacg acacaatcca gggacaaccg tgtaaatctc catcgtaata aacgccaagt
    42361 actaattcat tacatgacca ggccgttact ggagagagag catcatactc ttctaatccg
    42421 gggcaggtag aatccggtaa ggttgtatta ttgcgctcca tcctgagttc agggccggga
    42481 ggactcccgg tccaactaac taaaggcaaa cttgctgccg cagttgaatt agattcatac
    42541 gtgccatatt caactgattg aggggtcagg actgaaagat gaaaacgata ctttgcaaca
    42601 acgctggtcg tccccgcggg tacatagagt gtggcgatct ttgtcgcagc ctgtatccca
    42661 gaagcgcaca gagccagcac caataaacat ataacatgac gtattttatg cattttatat
    42721 atactcaaac gtagctgtca ggctggcggt gaaatcaccg atggttacat cacgaccgct
    42781 tttcgcctgg atccaggccc ggaaattaat atcattgttg ccgttggtaa gcgcaaaagt
    42841 cgccccggtg gtgttgttaa tatccacccg cttgccgtcg agcgtttcca tccctatccc
    42901 cacgccctgc gcggtggagg tgtcatcgac tgccagaaaa cccggctgac cgctgtcttc
    42961 cgttccggtg agggtgactt tgacgttgta tcccgccggc cctttgcagt ctttcagctt
    43021 aaacacaacc ggcagatgcg cggactgccc ggcgaccatc aaatcccggt tgctgacggt
    43081 gggaaaatga atttccgcca gattggcacc atccaccacc agcgtgcagg atttactcag
    43141 caggttgccg taaaaatgca ggttgttatc gactgcccgt gcccccgtgc tggcggtcag
    43201 cagcaggcct gctaacagcg cataaccggg tgaataaatg tgtgctgttt tcattggtaa
    43261 tccacccgta aggttgcgta agcagaaaaa ttcgtgtccg tcagcaccgc atcagccgct
    43321 ttgaccggta ccgcctccag ctttggcttc tggctaaaat cggtggcgtt aatggaaagc
    43381 ggctcgttga gcttaaaacg ctgaccgtca tgctgaagct caattccgaa gcccggtaca
    43441 tccgtctcaa tagcagagtc gttgtaggag gtggcagtcc ccgtccagga gagtgtcata
    43501 ttccactcgg gatcgcggac atccgagtcg cagctcagtt cgtagtccac tttctgacga
    43561 ccataattgc catcaatgct gtcgataata agatcgcgaa attgcacttc gatggtttgc
    43621 ccgccgctga tgctacaggc gggcggcgac atcagcgtac cgtggaatgt gatgtcatct
    43681 tccgccgcca gcacagcccc gcaaccagcg cacagtaaaa ggagcaggaa ttttttcatt
    43741 ggtaatccac caccatggtc atcgtggcgt taaactccga cggcgtcagt tgcaccccgc
    43801 tctgttttac cggcaccgct ttcagctgag gctgagtgtt gacattaaaa ggcgtccagt
    43861 tattttcacc aactgcaaac agggagttgt cggcggcgtt ttctatccgc acccccaggc
    43921 cggcgatatc cgtgctgaga accgtttctc cgttgatcac ggcggtattg cctttgagct
    43981 gcatccgtaa gtcatcgctg actttattac cgcagttgag ggtgtatttc gcatcctgtt
    44041 ggtaattggt accatcgatc ctgttgatta tcacattgcc aaatttcaca gcgcttccct
    44101 tcacggagca aggcggcggt ccatcgacca ggattcgcag cgtcagatcc acctgtttga
    44161 tatcactgcc agcaacagcc aaaccgctga tgccccatcc ggccagcacc agcaaccctg
    44221 cgcccgctac aaacttgtga ctcataagtc cctcccggca tcagcctttg cggctctgct
    44281 cttcatccac ctggcagctg ttgccggtgc atatgaagta cagcggcatc cgcgcgccgt
    44341 agtcgttgat gtaagtcagc accggcacgc tgtccatctt cgcattcagc ggtgcggagg
    44401 ttttcggctc catcatcagc ggtgtgaatc ccgccggcgg gttgccgcct tttttagcgc
    44461 tggcgttgct gatgatcacg tagtacggcg tcgggttatt gacggtgaag tcctggccgt
    44521 tacggatgag cgtcacctta tgctgccacg gacttttcat gctggtgttc tccagcgcct
    44581 tcggtcgcca gaacagtttg atacgcgtct gcagcgcaat ctgcagtgtg ttcggtttat
    44641 ttgatttcgg cggaatttcg cgcacgttga aatagaacac gctctcgcgg tcggccggca
    44701 gtttgttgat atccggcagc ccctgcacct tcacctggcc gttcatcatc gcatcgatgc
    44761 gctgcactgg cggaagcacc gcaagcggcg agctgatttt gttgcccttt gcatcctcta
    44821 tccagctctg cgccagataa ggcagtgtgg gatcgttgtt gcgcagcgtg acgctgatgg
    44881 acttgtcgct ttcgttaaac accagccgtg tcctgtcggg cgtcaccgaa gcgtggctgg
    44941 agaaactgac cgctgtcgcc agcagcgcca gtgccgggac aacgattttt gcagtgttag
    45001 tcagattaaa catggatcat tccttaatca cgttactggt ttactgaaat tgaagtcgga
    45061 ggttccgttg gcgtcacccg ctgtgtctgt tcctgaatcg ccggtttgac cgccggcgaa
    45121 gtgataggtg ctacattgcc tttatgctgg catggcagta acaagccgtt aaacagatcg
    45181 gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa caccactcca gaacacactc
    45241 atgtgttcgc caggtttcac cccagccaga taaacattgc cgtcatcatc gacaaggccc
    45301 actgtctgct cgttatcatt ttttacttct gcgccaaacg gaggatggct gccgtcgctc
    45361 aggcgcagca ccgccatcgc tttttgtccg ctaatgacag caaatttacg gtagccaatg
    45421 gcaccttcag ttagcgtggc ttgcaccacc gactgggttg cttcagcgtt ttcaggcaat
    45481 ctgttgaggt cgatatacgc ctgattgcgg taatagttat tgacgtcaga aacgacggct
    45541 ttaccaaaca tattggtata aacagcagcc ccgttacctt ccaccggaac atcggcaacg
    45601 ccatcggcat caatcaacaa gcgtgtcccg cccatattct gggtacggtg aagtgcgcca
    45661 ccgtgggcag taagcgttgc gccgccctgt aacgacaggc ccgcagaggt gtattgccct
    45721 tcatggtagt tcgcactgag gtcaacctgc gccagcgaac catcatggct gtaatagccg
    45781 tcaacgctgg tgtgtttgtc actggtgccg acgttcaact gatagtgagt cgcgtcatcg
    45841 acacggctga aataaccgac ctgactgctg tccgtcccac tgccatagtt accgttatag
    45901 ctaacggtac tgttgtcgcc ccacggcata ctgagcgaaa tgtacatgcc tttgtcggcc
    45961 tggttgtcat actcgtaacg gtagccagtc atcgagatgc tgacattacg aatactgccc
    46021 atattgaaat agtgcgagag catgatgttg tagtttgtct gctcctcgcg atcccagtag
    46081 gtatggcggg tatagttgag ataaaccgaa acacccgcat cgcggaagtt ctggttataa
    46141 gtggcggtgt acatctcttt gtcgttgccc gtgcgtacca ttccgctgtc actggcatcc
    46201 agatactcgc tcatggtcat aaagttctct tccgagaagc gatatccagc aaaagtaacg
    46261 cggctgttga gctggtcaaa gtctttggaa taactcacac ggaaggagtt accgtccagc
    46321 gaacctttgc cataagcggt gtctttatcc agtttggtgt gcgagtgagt aacatcaaac
    46381 gcgaccgcgc cgaatgtaga caaatcgcga ccgacgccaa gcgccgcaga ctgatagttt
    46441 tcatctccca gtgcgccgcc atataacgac cagccgttag cgatccccca ggaagcttca
    46501 gcaccagaaa aaaattcacc ctcgacatgg tgtccccact cttgcggacg gcccatcatg
    46561 atcttatagc gaacctgacc tgggcgagtg aggtatggca tcgaggcggt gctgatgtca
    46621 tattcctgca cctggccgtt ctgttcttca atgcgaatat gcaacgtacc ggagacagaa
    46681 tcaccaagat cctgaatacg aaacggtcca gccggaacct gcgtttcgta aatcacacgc
    46741 cccatctggc tgacggtcac ttttgctgtg gtgtgtgcca cgccggaaat gtctggcgcg
    46801 tagccgcgca gattgggagg caacatttga tcgtcagtac tgacactgcc accaacatag
    46861 ttaaaaccat caaaaatatc ggatctgagg taatcctcgc ccagcgccag tttggctttt
    46921 aatgatggta acgcccgcca ggcatagtag cgactccact cccatttttt ttgagtttca
    46981 tcgccgctga attcatcgtc atcattactg cgagtatgtt gatagttagt ctgccagtca
    47041 gcacgcatac gccacggccc caggttaacc ccgaccgtcc cgttgccgct gatctcgtta
    47101 ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt tgatgctgta gtccgcgacg
    47161 atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc agtcggggta agtatattcg
    47221 aggtaagcct gtggcagtga aatgactaat gcggactggc ttaaatcagc cttaatttcc
    47281 atgccttcca gttgaccgga tttcaggcat ttagcatcgt ggctccattg cagatttttc
    47341 gccacgtctt cttttaaacc aaactgcgct accagttccg gtgtcagaca agcatagctt
    47401 ttgctcgcgt catcttcacc agcataccag taaatatcgt actcttccgc caatggctgt
    47461 ttatttagtt gaacctgtaa attgtatttt ccgggctcaa catatccctg gctggaaaaa
    47521 cgcttcagat caatttttgt gtcgcctttt aattccagaa aacgggaatc aaactgaatg
    47581 tcgtcttcag cccagacact gctataactc cctgacattg ccagcgcaat gcaccacgga
    47641 agaatccgta atcgaaaaag agaatggtca ggcatacacg tttctatccg tttttattct
    47701 tgttcggtca aaatccattt aaatccccca tagcagtgtc gctatggggg tatgccttac
    47761 aggtaagtaa tctggaaggt ggtatttgct tcgaaattac ccagatctgg cgcatcagct
    47821 gcgccgacca gccaggcttt aaagttcagt gtctgcttcg ctttgccttt attggtagaa
    47881 gtgtctttca ctattttctg gtcaataccc atgccgcttt tgtaagaggt acccagagag
    47941 tcaccgatcg ccaggctaac attgttaaat gccgcaccgg tatcggtatt gaaaatggtg
    48001 taataattgc cgctatttgc agaagaaacg gtaccagtaa aggtagtggt cattgtttcc
    48061 tgagtatcaa atacgcaatc ttgcagacga atctggaaac ttttcggcgt ggtggtgccg
    48121 ttatttttca gtttttcggc accgatatca cccatatcaa cttccagttt gtgatcgtcc
    48181 ggaacgatag aacacactga gtcttcaatg gtgccgtaaa aacgtgcagt accgttgtta
    48241 cccgcagcga ttgttgccgt agaggtgaac acgcccatca ccattgccgc agcaatagct
    48301 gttttaacaa acttactcat tatttgtcct taaactataa ataattaaaa atataaacat
    48361 tgcaatacat tgattcagtc aatagccaat gtttataccc agccattgaa tacggcatgc
    48421 gatccaaaaa gaacaggtcg tagaaaattg tattcgttgg tgtggggc
//
LOCUS       DAFAVF010000018        47798 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFAVF010000018 DAFAVF010000000
VERSION     DAFAVF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.018"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            96..728
                     /gene="comR"
                     /locus_tag="LGK42_001008"
     CDS             96..728
                     /gene="comR"
                     /locus_tag="LGK42_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBK2657284.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(790..1329)
                     /locus_tag="LGK42_001009"
     CDS             complement(790..1329)
                     /locus_tag="LGK42_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBK2657285.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1556..2860)
                     /gene="ndh"
                     /locus_tag="LGK42_001010"
     CDS             complement(1556..2860)
                     /gene="ndh"
                     /locus_tag="LGK42_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBK2657286.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3268..3810)
                     /locus_tag="LGK42_001011"
     CDS             complement(3268..3810)
                     /locus_tag="LGK42_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2657287.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3833..4858)
                     /gene="nagZ"
                     /locus_tag="LGK42_001012"
     CDS             complement(3833..4858)
                     /gene="nagZ"
                     /locus_tag="LGK42_001012"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBK2657288.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4869..5693)
                     /gene="thiK"
                     /locus_tag="LGK42_001013"
     CDS             complement(4869..5693)
                     /gene="thiK"
                     /locus_tag="LGK42_001013"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBK2657289.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5674..6315)
                     /gene="lpoB"
                     /locus_tag="LGK42_001014"
     CDS             complement(5674..6315)
                     /gene="lpoB"
                     /locus_tag="LGK42_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBK2657290.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6329..6706)
                     /locus_tag="LGK42_001015"
     CDS             complement(6329..6706)
                     /locus_tag="LGK42_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBK2657291.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6709..7068)
                     /gene="hinT"
                     /locus_tag="LGK42_001016"
     CDS             complement(6709..7068)
                     /gene="hinT"
                     /locus_tag="LGK42_001016"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBK2657292.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7402..9591
                     /gene="fhuE"
                     /locus_tag="LGK42_001017"
     CDS             7402..9591
                     /gene="fhuE"
                     /locus_tag="LGK42_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBK2657293.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTQGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9651..11084)
                     /gene="ptsG"
                     /locus_tag="LGK42_001018"
     CDS             complement(9651..11084)
                     /gene="ptsG"
                     /locus_tag="LGK42_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBK2657294.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11379..12176)
                     /locus_tag="LGK42_001019"
     CDS             complement(11379..12176)
                     /locus_tag="LGK42_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2657295.1"
                     /translation="MFLADSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12187..13191)
                     /gene="holB"
                     /locus_tag="LGK42_001020"
     CDS             complement(12187..13191)
                     /gene="holB"
                     /locus_tag="LGK42_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK2657296.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13188..13829)
                     /locus_tag="LGK42_001021"
     CDS             complement(13188..13829)
                     /locus_tag="LGK42_001021"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK2657297.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13819..14841)
                     /gene="yceG"
                     /locus_tag="LGK42_001022"
     CDS             complement(13819..14841)
                     /gene="yceG"
                     /locus_tag="LGK42_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBK2657298.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14844..15653)
                     /gene="pabC"
                     /locus_tag="LGK42_001023"
     CDS             complement(14844..15653)
                     /gene="pabC"
                     /locus_tag="LGK42_001023"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBK2657299.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15773..17014)
                     /gene="fabF"
                     /locus_tag="LGK42_001024"
     CDS             complement(15773..17014)
                     /gene="fabF"
                     /locus_tag="LGK42_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK2657300.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17102..17338)
                     /gene="acpP"
                     /locus_tag="LGK42_001025"
     CDS             complement(17102..17338)
                     /gene="acpP"
                     /locus_tag="LGK42_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK2657301.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17549..18283)
                     /gene="fabG"
                     /locus_tag="LGK42_001026"
     CDS             complement(17549..18283)
                     /gene="fabG"
                     /locus_tag="LGK42_001026"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBK2657302.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18296..19225)
                     /gene="fabD"
                     /locus_tag="LGK42_001027"
     CDS             complement(18296..19225)
                     /gene="fabD"
                     /locus_tag="LGK42_001027"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK2657303.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19241..20194)
                     /gene="fabH"
                     /locus_tag="LGK42_001028"
     CDS             complement(19241..20194)
                     /gene="fabH"
                     /locus_tag="LGK42_001028"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBK2657304.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20262..21332)
                     /gene="plsX"
                     /locus_tag="LGK42_001029"
     CDS             complement(20262..21332)
                     /gene="plsX"
                     /locus_tag="LGK42_001029"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK2657305.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21413..21586)
                     /gene="rpmF"
                     /locus_tag="LGK42_001030"
     CDS             complement(21413..21586)
                     /gene="rpmF"
                     /locus_tag="LGK42_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK2657306.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21638..22159)
                     /gene="yceD"
                     /locus_tag="LGK42_001031"
     CDS             complement(21638..22159)
                     /gene="yceD"
                     /locus_tag="LGK42_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBK2657307.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22358..22942
                     /gene="yceF"
                     /locus_tag="LGK42_001032"
     CDS             22358..22942
                     /gene="yceF"
                     /locus_tag="LGK42_001032"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBK2657308.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23054..24013)
                     /gene="rluC"
                     /locus_tag="LGK42_001033"
     CDS             complement(23054..24013)
                     /gene="rluC"
                     /locus_tag="LGK42_001033"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBK2657309.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24586..27771
                     /gene="rne"
                     /locus_tag="LGK42_001034"
     CDS             24586..27771
                     /gene="rne"
                     /locus_tag="LGK42_001034"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBK2657310.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAIEPVVSAPVIEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVTQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27968..28921)
                     /gene="flgL"
                     /locus_tag="LGK42_001035"
     CDS             complement(27968..28921)
                     /gene="flgL"
                     /locus_tag="LGK42_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBK2657311.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28933..30576)
                     /gene="flgK"
                     /locus_tag="LGK42_001036"
     CDS             complement(28933..30576)
                     /gene="flgK"
                     /locus_tag="LGK42_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBK2657312.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30642..31583)
                     /gene="flgJ"
                     /locus_tag="LGK42_001037"
     CDS             complement(30642..31583)
                     /gene="flgJ"
                     /locus_tag="LGK42_001037"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBK2657313.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31583..32680)
                     /gene="flgI"
                     /locus_tag="LGK42_001038"
     CDS             complement(31583..32680)
                     /gene="flgI"
                     /locus_tag="LGK42_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBK2657314.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32692..33390)
                     /gene="flgH"
                     /locus_tag="LGK42_001039"
     CDS             complement(32692..33390)
                     /gene="flgH"
                     /locus_tag="LGK42_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBK2657315.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33443..34225)
                     /gene="flgG"
                     /locus_tag="LGK42_001040"
     CDS             complement(33443..34225)
                     /gene="flgG"
                     /locus_tag="LGK42_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBK2657316.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34363..35118)
                     /gene="flgF"
                     /locus_tag="LGK42_001041"
     CDS             complement(34363..35118)
                     /gene="flgF"
                     /locus_tag="LGK42_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBK2657317.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35138..36343)
                     /gene="flgE"
                     /locus_tag="LGK42_001042"
     CDS             complement(35138..36343)
                     /gene="flgE"
                     /locus_tag="LGK42_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBK2657318.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36368..37063)
                     /gene="flgD"
                     /locus_tag="LGK42_001043"
     CDS             complement(36368..37063)
                     /gene="flgD"
                     /locus_tag="LGK42_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBK2657319.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVMRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37075..37479)
                     /gene="flgC"
                     /locus_tag="LGK42_001044"
     CDS             complement(37075..37479)
                     /gene="flgC"
                     /locus_tag="LGK42_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBK2657320.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37483..37899)
                     /gene="flgB"
                     /locus_tag="LGK42_001045"
     CDS             complement(37483..37899)
                     /gene="flgB"
                     /locus_tag="LGK42_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBK2657321.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38054..38713
                     /gene="flgA"
                     /locus_tag="LGK42_001046"
     CDS             38054..38713
                     /gene="flgA"
                     /locus_tag="LGK42_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBK2657322.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38789..39082
                     /gene="flgM"
                     /locus_tag="LGK42_001047"
     CDS             38789..39082
                     /gene="flgM"
                     /locus_tag="LGK42_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBK2657323.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39087..39503
                     /gene="flgN"
                     /locus_tag="LGK42_001048"
     CDS             39087..39503
                     /gene="flgN"
                     /locus_tag="LGK42_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBK2657324.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39543..41078)
                     /gene="murJ"
                     /locus_tag="LGK42_001049"
     CDS             complement(39543..41078)
                     /gene="murJ"
                     /locus_tag="LGK42_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBK2657325.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41188..42111)
                     /locus_tag="LGK42_001050"
     CDS             complement(41188..42111)
                     /locus_tag="LGK42_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2657326.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42113..42760)
                     /locus_tag="LGK42_001051"
     CDS             complement(42113..42760)
                     /locus_tag="LGK42_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBK2657327.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42771..43355)
                     /gene="rimJ"
                     /locus_tag="LGK42_001052"
     CDS             complement(42771..43355)
                     /gene="rimJ"
                     /locus_tag="LGK42_001052"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBK2657328.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43591..44799
                     /gene="mdtH"
                     /locus_tag="LGK42_001053"
     CDS             43591..44799
                     /gene="mdtH"
                     /locus_tag="LGK42_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBK2657329.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44863..45510
                     /gene="grxB"
                     /locus_tag="LGK42_001054"
     CDS             44863..45510
                     /gene="grxB"
                     /locus_tag="LGK42_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBK2657330.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45644..46204
                     /locus_tag="LGK42_001055"
     CDS             45644..46204
                     /locus_tag="LGK42_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2657331.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46310..47356
                     /gene="pyrC"
                     /locus_tag="LGK42_001056"
     CDS             46310..47356
                     /gene="pyrC"
                     /locus_tag="LGK42_001056"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK2657332.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47430..47675
                     /gene="dinI"
                     /locus_tag="LGK42_001057"
     CDS             47430..47675
                     /gene="dinI"
                     /locus_tag="LGK42_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBK2657333.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11560 a        12905 c        11894 g        11439 t
ORIGIN      
        1 agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata
       61 acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca acacaatgtg
      121 taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca
      181 tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga
      241 ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg
      301 ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg
      361 aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta
      421 ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag
      481 attcaggaga tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc
      541 agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac
      601 atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg
      661 catcgctgga aaaactaatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg
      721 tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt
      781 acagccttgt tatactttgt tattcaaaat cagttgccca ttactatcta gcgggatctg
      841 cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac
      901 atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt
      961 cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc
     1021 cagcgccccc acaacagtgg cgacatcttt accacgacca ccaccaaact gatgccctat
     1081 cacgccgcca gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg
     1141 tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt
     1201 gattggggtt gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca cgttcagact
     1261 ggccactgcc gctacgccca gcgcagctgc gacaccaatc ccgatacccg ccaacattga
     1321 tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa
     1381 agcagctatc agacagaaga tgaaaagcgc gggaatggct ggaaatactt aaggattcgg
     1441 aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt
     1501 accatttgtc cagggcggat gaggcgttta tgccacatcc gccagtgtac gtcgattaat
     1561 gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg
     1621 ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc
     1681 gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc aggctaccga
     1741 cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga taatttttca
     1801 gcggcttacc gttcatctgc gccagtatgt tgttcattgc gcaagtcgcc atctggtgtg
     1861 cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac gcgcagtcgc
     1921 caatagcgta aatgtctgga tcgcgggtgg tttgcagcgt cggttccacc accagctggt
     1981 tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg
     2041 ctgcccacac catcagatca gcctcaatat attcgccatc tttagtgtgc aggccgcctt
     2101 catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt
     2161 tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct cccgcttcta
     2221 ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg tgcagttgct
     2281 tgaccgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg
     2341 caatgttcac tttgccattt gcgcccaggt tggcggagta tttcaggaag agattcagca
     2401 tctcctggtg aaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga
     2461 cacctggcgt attgaaatcg ttagaggtgc tacccagcgc cattaccagg gtgtcatagg
     2521 cgattttacg ttccggaacc agcagttcac ctttctcatc gcgcagttct gcaatagtga
     2581 ttgttttcgc ttcacgatca atatcaatga cggaacccag ctggaactgg aaaccatgat
     2641 tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca
     2701 cttcgtgcag cagcggtttc cacaggtggc tgtggttacg atcgaccagc gtaattttgg
     2761 cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac
     2821 cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc ttaaatttat
     2881 taaccaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag gttacagcaa
     2941 tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata ccaaaattga
     3001 tgttaatcaa gttttgttgc tgaaaagata ggcgacttta ccgggtgaga tcaaaaaata
     3061 gagtttattg tttattattt gagcaaattc ggaagagtaa tatagggtga agcgtatatc
     3121 gagggcgttt ggtcgtcctg ataagatgcg cagcatcgca tcaggcattg atgctcgact
     3181 gtcggatgcg gcgtggacgc cttatccgac ttacagaatt cggagcgcat caggtaccgg
     3241 taaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
     3301 taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
     3361 ataatgatgc aactcttcag atgtccgctg gctgttaagt gcttcatcat tacgcgacaa
     3421 aataaccaga caacgatcgc gattcttctc gcggaagttg gtcacacact tagtggcaat
     3481 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
     3541 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
     3601 gcccaggcca acgccgcaaa ttagcggacg ctcgtcaacg ttcagttgca acattttgtc
     3661 cacttcttta agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
     3721 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
     3781 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
     3841 ctgctttctc ttcctgccag cgttcgtgta actgattcag acgggtgctg atcgctttcc
     3901 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
     3961 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
     4021 tgcagaccag gatcatatcg caacccgcat ccagtgatgc ctgcccgcgt tcggcataac
     4081 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
     4141 ccagttcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc ggacgcggat
     4201 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
     4261 aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg
     4321 gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg
     4381 ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt
     4441 gtggatcggc atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acatccagca
     4501 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
     4561 cctcttgcgc cagtttgccg ccctcttcca ttcctgacag cgcagcgaat gattgcgccg
     4621 ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg
     4681 ccaccaccag atgattgcgc gaagctgcgc ggatctggcg caccagttca cgtaactggg
     4741 caggatcatg atagttacgc gtaaagagaa tcagccctcc caccagcgga tgcgcaagta
     4801 tttcacgctc ttccgcgtcc agttcgtaac ctttgacatc caacattact ggacccacac
     4861 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
     4921 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
     4981 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
     5041 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac cgccgccagt
     5101 tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg
     5161 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
     5221 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
     5281 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
     5341 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
     5401 gtttttaccg cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
     5461 ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
     5521 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
     5581 agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
     5641 aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc tgcgaaacgg cacctttacc
     5701 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
     5761 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
     5821 tatggcttta ctacgggtac ctaaactgtc ctgcggcgac aaacctaact gttgcttagc
     5881 catcgacagc tgctgggcgg aaaccagtgt aaatttcccg ttattagcca gcgcatttcg
     5941 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
     6001 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
     6061 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg ccgtttgatc
     6121 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
     6181 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
     6241 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
     6301 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
     6361 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atgctaacgc
     6421 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     6481 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
     6541 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     6601 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     6661 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     6721 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     6781 ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc
     6841 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     6901 agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
     6961 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     7021 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     7081 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     7141 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
     7201 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
     7261 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttacaaacaa
     7321 aattattcgc acactaaata taatcgctca tcaccctcct ccggcatgag cctgacgaca
     7381 acataaacca agagatttca aatgctttca acacaattta acagggataa tcaatatcaa
     7441 gccatcacca aaccgtcact acttgccggt tgcatagcac tggcactatt accttctgcc
     7501 gcttttgctg caccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca
     7561 gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg
     7621 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
     7681 cagttacaaa cgctgggcga agtgatggaa aacacgcagg ggatcagcaa aagtcaggcg
     7741 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
     7801 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
     7861 ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat
     7921 ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat
     7981 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
     8041 ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca
     8101 tggctggacc gctacaacag tgaaaagacc ttcttctcgg gcattgtcga tgctgattta
     8161 ggcgatctta cgacgctttc agccggttac gaatatcagc gcattgatgt taatagccct
     8221 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgtgca
     8281 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
     8341 ctgaagcagc ggtttgctga tacctggcaa gcgacactga atgccaccca ctctgaagtc
     8401 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
     8461 gttgggccat acagtaatta tggacctggc tttgattatg tcggcggcac cggttggaac
     8521 agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtagtta tgaattgttt
     8581 ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa tcgttacttc
     8641 agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat
     8701 ttcccacaaa ccgactggtc accacagagc ctggcgcagg acgataccac acatatgaaa
     8761 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
     8821 cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
     8881 ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc
     8941 tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc
     9001 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
     9061 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
     9121 ggcagcaacg gcgaaaccgc ctataaagca gtggatggga cagtcagtaa aggggtggaa
     9181 tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
     9241 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
     9301 atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac
     9361 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
     9421 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
     9481 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga aggttctatc
     9541 gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg aataacacct
     9601 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacaga ttagtggtta
     9661 cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa aatcgcctga
     9721 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc ggcctgatcc
     9781 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
     9841 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtc
     9901 gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
     9961 agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat cgggaacagc
    10021 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
    10081 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
    10141 tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata
    10201 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt
    10261 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
    10321 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
    10381 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
    10441 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
    10501 aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca
    10561 atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg
    10621 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
    10681 aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag tacgccagta
    10741 tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
    10801 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
    10861 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
    10921 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
    10981 gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc
    11041 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
    11101 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta
    11161 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt
    11221 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
    11281 tatgtcacaa tatttcaaac gtggttattc accgcccaat tttcggcggt gaactttact
    11341 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
    11401 gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
    11461 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
    11521 ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat
    11581 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
    11641 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
    11701 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
    11761 agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca
    11821 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa
    11881 taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa
    11941 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
    12001 ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact
    12061 gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
    12121 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcggctaa aaacatgatg
    12181 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
    12241 cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtaacagaca ttaactgttc
    12301 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
    12361 cagttcggcg accaggcccg gcacatcaac attagtcacc tgcgcagcac catgatggcg
    12421 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg
    12481 attaagggcc gctagcagcg aataccagtc gcccgatggc acgctatatg ccaacgcctg
    12541 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg
    12601 cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg
    12661 tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg
    12721 actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc
    12781 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc
    12841 agcatcggtt acccagacaa ctttcgcacc acctaagcgt gcgtgctcat tcagcttttc
    12901 ggtgacctca cgtaccgcat caatgcccag catatttttc cctttttcgg gagccagggt
    12961 gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    13021 tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    13081 atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata
    13141 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    13201 tccttaaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    13261 gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg
    13321 gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc
    13381 gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa
    13441 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg
    13501 ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    13561 agagctggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact
    13621 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    13681 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    13741 agttgctcaa gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc
    13801 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    13861 cacagacttg ttatggctgg caagattggt attaaacgtg tgaccacctt taccatcggc
    13921 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    13981 cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg
    14041 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    14101 ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac
    14161 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag
    14221 ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    14281 tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat tggcggtata
    14341 catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    14401 ctgcgctacg gtggcgtact tatcgtcgct cagcgtatgc ttgatatacg gggcctcacg
    14461 caattgcttg aggtaatcgc tcagacgcat cccttctacc agtcgcagag ggaactgtgc
    14521 ttctttaccg ctttccagca acttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    14581 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac
    14641 ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt
    14701 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    14761 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    14821 tatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    14881 tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt
    14941 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga agcttgcact
    15001 tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg
    15061 ttaacacctg cctgatccag tcgcggcgta taaactacgt tgcccttccg ccagaacaaa
    15121 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    15181 tttgtctgct caagatgaga gcgaatcaat acttgctcaa gacgattgag atgtttaata
    15241 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccctcg
    15301 ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg cgttgccggt
    15361 ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagt
    15421 acaccatttt gctgttctgc tgcaagcgtt ttcatctctt gttcaagctg tggccagaag
    15481 tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac
    15541 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    15601 gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct
    15661 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    15721 ctaagaaaaa aggcccgcaa gcggaccttt tataagggtg gaaaatgaca acttagatct
    15781 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt
    15841 ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg
    15901 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga
    15961 tagattctac tgcacccgcc gcacctaaca gatgaccggt catagatttc gtggagctta
    16021 ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    16081 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    16141 ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg
    16201 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    16261 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    16321 gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    16381 gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    16441 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatgac
    16501 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    16561 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    16621 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    16681 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    16741 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    16801 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    16861 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    16921 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    16981 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat
    17041 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    17101 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    17161 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    17221 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    17281 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    17341 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    17401 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    17461 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    17521 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    17581 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    17641 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    17701 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    17761 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    17821 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    17881 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    17941 aaacagatga aaggttggtt tcgataatat cgttccactc ttcatctttc attcgcatta
    18001 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    18061 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    18121 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    18181 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    18241 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    18301 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    18361 caatgcgttt cgtcaggcca gtaagcactt tgcccgggcc gacttcatag agatgttcta
    18421 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    18481 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    18541 caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    18601 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agagcgcgtt
    18661 ttgcgcccgc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    18721 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    18781 cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcgcccg
    18841 tgccttccgg tacggcttct tgcataaact tgccgcgcat ctcaaccaga cgcaccgcat
    18901 cagcgaaatc aatcacacca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    18961 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagagg
    19021 cagtcaacag cgcaggctga gtttgccagg ttttattcag ttcttcagct ggcccctgct
    19081 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    19141 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    19201 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg
    19261 agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    19321 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    19381 ggcgatccag cgtcaccacg acattgtcca tagacatacc gagttttttc gccgttgcac
    19441 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    19501 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    19561 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    19621 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    19681 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    19741 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    19801 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc
    19861 aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc cagcatgctt tgaatctgac
    19921 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    19981 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    20041 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    20101 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    20161 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    20221 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    20281 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    20341 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    20401 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    20461 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    20521 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    20581 gaaaggaaca tcctgacaac accttccatc gtctttaatg tgacatttcc tgtaaagccg
    20641 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    20701 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc
    20761 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    20821 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    20881 acgttggccc ctaagtcaag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    20941 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    21001 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    21061 agggccacgc gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    21121 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    21181 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    21241 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    21301 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    21361 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    21421 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    21481 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    21541 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    21601 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    21661 atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag accatgtccg
    21721 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    21781 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    21841 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    21901 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    21961 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    22021 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    22081 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    22141 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    22201 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    22261 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    22321 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    22381 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    22441 accagaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    22501 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    22561 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    22621 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    22681 gtttaattcg gcgaatgggc atctgcaaac agaagtggag ccttttgacg tccatttccg
    22741 tcacctgagc gaggcggaga ttgataatta cgtgcgtaaa gagcatcccc tgcactgcgc
    22801 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag aggggcgtga
    22861 tcctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    22921 gaaaaacccg ctgatgggat agttttttgc gccggatgcg gttacttcgc atccggcaat
    22981 cggtgctggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    23041 ttatctgctt gtattagcgc gcgttacgca gcttttgcaa acaacgtttc aaaccttcat
    23101 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    23161 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    23221 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    23281 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    23341 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    23401 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    23461 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    23521 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    23581 ggtcgagacg atgaaccagt tcaagaaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    23641 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    23701 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    23761 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    23821 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcgc
    23881 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    23941 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    24001 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    24061 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    24121 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    24181 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    24241 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    24301 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    24361 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    24421 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    24481 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    24541 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    24601 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    24661 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    24721 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    24781 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    24841 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    24901 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    24961 tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg tatcgaaggc
    25021 gacgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    25081 cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc
    25141 ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggccccgttc
    25201 ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac
    25261 atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct
    25321 gcattaggtc gcccggattt cagcagcaaa atcaaactat acaccggcga gatcccgctg
    25381 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    25441 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacggccat cgacatcaac
    25501 tccgcacgcg cgacccgcgg cggcgatatc gaagaaaccg cgtttaacac caacctcgaa
    25561 gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    25621 gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    25681 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    25741 gaaatgtccc gtcagcgcct tagcccatca ctgggtgaat ccagtcatca cgtttgtccg
    25801 cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    25861 ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    25921 ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt
    25981 caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc gcactaccac
    26041 gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg
    26101 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa
    26161 caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac gccagctgaa
    26221 cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc agcaactcct
    26281 gcacaaccag ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt
    26341 gaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa
    26401 caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc
    26461 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    26521 gcacagcagc agactgccga gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa
    26581 gcgcgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaatgat
    26641 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    26701 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    26761 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    26821 gaagaaactg ccgctgccga accaattgtt caggaagcgc cagctccacg cacagaactg
    26881 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    26941 gctgataacc gtgacaacgg tggtatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    27001 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    27061 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    27121 tatccaattg tacgtccgca agatgtacag gttgaagagc agcgcgaaca ggaagaagta
    27181 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctatcgaacc ggttgttagc
    27241 gcgccagtta ttgaagaagt ggctgaagtc gtagaagccc ccgttcaggt tgccgaaccg
    27301 caaccggaag tggttgaaac gacgcatcct gaagtaattg ccgccgcggt aactgaacag
    27361 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagttgcaga acacgcagaa
    27421 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    27481 gttgtggttg ctgaacctga agttgttact caacctgccg cgccagtcgt cgctgaagtc
    27541 gcagcagaag ttgaaacggt agccgcggtt gaaccagaga tcaccgttga gcataaccac
    27601 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    27661 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    27721 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    27781 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    27841 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaaatgc
    27901 ccgccccggc gatcccagag cgggcagaca aaacataccc agtttcatga tatgttttaa
    27961 agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat
    28021 acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca
    28081 catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaaggaat
    28141 ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacagtc agcacattgt
    28201 tcagcgagtt tttcagtccg cggttggttt tatctaacgc cgcagcggcg gtttctttat
    28261 ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcactatcc agcatggcaa
    28321 aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttgctg gtaatactgt
    28381 cgaaaatttt gtcacccgtg tgccctatca ccatcgaacg cgaagcatcg acctgttgtt
    28441 taatactttc tgctccaccg acgtatttcc ctttctcttc gctaaacggc gcagtctctg
    28501 ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca
    28561 actggtcacg aagcccctga atatccgtag ccagcgaggc ccggtcatcg tcactcaagg
    28621 tgccattgct ggcgtagaca attttttcct gagcattctg gatagcagtg gtgacctggc
    28681 taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgtgcc agcgtgtact
    28741 ggctgttttg cgcctgtgcc tgggagagaa ctacggcttg tgatgctgca atgggatcgt
    28801 cagaagggtt aacgactcgc ttacccgtcg acatctgctc gccgtacttc atccattctg
    28861 cctgagaatt ggtgatgccg cgcatgtttt gctggtacat catctgggta ctgaaacgca
    28921 ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag
    28981 aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt actcctcatc
    29041 gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca cattaccttg
    29101 cgtggtgcta ctggttttca acgtcgcggt tttattaccg atatcactca ctaacgaggc
    29161 ataagcgtcg ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt gcagatccag
    29221 cagggcctga ccgttgcggt tgtcgctatc acccgcatct tcttcgctcg ccatcgcgat
    29281 tttcgcttcg tcggtgatta atacatccat gttgacgatg gcgtcactta ctggtttcag
    29341 cgtgaagctg tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc
    29401 cactttaccg ttggcatccg gcgtcaccgt aaaagtggta ttgctggcaa ggcgggtgac
    29461 ctgccactga ttattatcga acgagatttt gtaatctgtc gccagtaccg cggaggcatc
    29521 agttaccgtg gcaccgatcg caacgtcacc tttgtttttc gtgttttgca gaaccgcggg
    29581 cttaccgata gcaaagaaat cttcaccagc atcgccgttg gcatcaaatc cggctttgtg
    29641 ttggctgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat tacgcgtctg
    29701 gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt
    29761 ctccgggatc tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg tacgagaagg
    29821 gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa ccagtgagta
    29881 accattggcc atcgtgatgt tataagtacc gccatcctga acgctgactt ctacgccaac
    29941 aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc
    30001 ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc
    30061 gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc
    30121 gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag
    30181 cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa
    30241 atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga
    30301 catctgctca tagcgggcgg tcaggccgct actttgcgtc tgcgcagcac gtaactggtt
    30361 ggtaataaac gcatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca
    30421 gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc
    30481 ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt
    30541 cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat
    30601 tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg
    30661 ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg gatcatgttg
    30721 gtgagtttac gggcatagtg aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc
    30781 ccctgttccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg cgttaacagc
    30841 ccaacgtaat ccgacaaggc ttccagatac gagctgtaga cgcgaaactt cgcttttact
    30901 ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtaactgg ccctttccag
    30961 ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc gcggcggatt
    31021 tgccgttgcc cccaaccaga ttccagtgcc gcctgagcga ggatcaaatg atgtggcaca
    31081 ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctttactg
    31141 tcacccggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag ctgcgaaagc
    31201 gcctgatttt gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg
    31261 gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc
    31321 agacctttgc ccgccgtcat ctgttgggca atctgctggt catacatact ggtatacaga
    31381 cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac
    31441 atcatctgca cgaacatccc ttccacctgg cgggccaccg gacggatatt tgccgccgga
    31501 tcttcgcccg ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc
    31561 agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac
    31621 tttgcattga ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg
    31681 cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct
    31741 ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca aacggtgtat
    31801 ctggctggct gacattggcc tgacgattga ctgttactga gagatttccc tgcgctaccg
    31861 cgcagctgtc gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca
    31921 ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatct gcaaggaaac
    31981 ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca tctaacgcgg
    32041 tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga
    32101 aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt
    32161 cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggttc acctgaacac
    32221 tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat
    32281 acacctgact gtcaacgccc ttaagcggcg tcatcaacaa cgtacctcca cgcaagcttt
    32341 tggcatttcc catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag
    32401 gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg
    32461 gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct
    32521 gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca atcagtgagt
    32581 tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg
    32641 taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg
    32701 cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc
    32761 attgcctacg tattcaatgc gcgcatccgc cacctgagta gacggtacgg tattgctgcc
    32821 gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat
    32881 ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt
    32941 caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc cgttgaacgt
    33001 gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg
    33061 cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct
    33121 tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg
    33181 tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat
    33241 agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac
    33301 cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat
    33361 ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg
    33421 tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg
    33481 tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt
    33541 cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt
    33601 tcaggcccgg cgtgctttcg ttcggtgcac cagaggattg cgtttcggtg tagaggtttt
    33661 cgccaatgct ctccagcccg gtgtcattca taaaggtggt gagattgagc tgcccaacct
    33721 gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga
    33781 tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca
    33841 ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct gatgaaccat
    33901 ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttgttgg
    33961 tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc
    34021 cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa
    34081 tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga
    34141 cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt
    34201 tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattagtga caagcagagc
    34261 agaagcacgc gacagtgtga atttcccctc accctaaccc tctccccaaa ggggcgaggg
    34321 aatagtccgc gcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag
    34381 ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa
    34441 acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc
    34501 cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtaccggcc cacgcgtggc
    34561 ctgggtttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt
    34621 ggctttcacc agtttcagac gccccactgg cgcaaccgta tttgccggat cgcccggatt
    34681 gagcgccgag attgtgccat cggcagcaat agtgatttcc gccccttccg gcacagcaat
    34741 tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac
    34801 ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca
    34861 gccatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt
    34921 catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtaggcagtg aaagcccttc
    34981 caccggcact gcacgcaagg cgtttaactg tgcgcgaaaa ccgggcgttg aggcattggc
    35041 cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcggt
    35101 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    35161 tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    35221 cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccaac gcaccgttgg
    35281 tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    35341 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    35401 gtacaatctg ccccagcagt tgtgtttgtt cgttggaata gttgccgaca accgtaccgt
    35461 catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca
    35521 caatattgtt agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg
    35581 cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt gaggtcagaa
    35641 cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt ggatcactgc
    35701 tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtac acgctcatgt
    35761 catgagcatt accctgacta tcgaaaacag tcaccgaacc ttttttgtta tagctatccg
    35821 cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct
    35881 gcatcgatgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg
    35941 tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct
    36001 gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc
    36061 tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa
    36121 cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    36181 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    36241 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    36301 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    36361 tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    36421 ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    36481 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg
    36541 gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtgaact ccggcggtca
    36601 gttcaccaat atcaatggtg cgcataaccg caccattttt atcggtgatg gtggccgtaa
    36661 ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagctcctt
    36721 cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccgatca
    36781 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagtgtgg
    36841 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    36901 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa
    36961 aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    37021 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    37081 accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt caacgttggc
    37141 ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg
    37201 catttttacg tagccctttg catctgccag cggattaccc ggttcataaa ccagtttgtc
    37261 cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc
    37321 agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcag gaccggtcac
    37381 gctatcagca tttgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa
    37441 cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa
    37501 aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag
    37561 gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga
    37621 aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg
    37681 gatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc gtccacgttg
    37741 catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc
    37801 ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc
    37861 ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa
    37921 attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg
    37981 ggtcgctatt tatgccgttg atggtcattg cggacaggta caattcacgt tgtagaaatg
    38041 gctgggggcg aaaatgctga caataaaacg tagcgtggcg atcatcgcga tactgttcag
    38101 tccgttaagt acggcgagca atctcacatc gcaattgcac aactttttta gcgcccagct
    38161 cgcgggggta agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc
    38221 gtgcgagcag ccattgcttt cgatgagcaa taattcccgc ctgtggggca atgtgaatgt
    38281 gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtacagg ccacaggaaa
    38341 ttatgtggtt gccgcgatgc ccattgcgcg gggaggaaag ctggaagctg gcaatgtcaa
    38401 actgaaacgc ggacggctgg ataccctgcc accgcgtacg gtgctggata tcaatcaact
    38461 tgttgatgcc attagcctgc gcgatctatc acccgatcaa ccaatccagt taacccagtt
    38521 tcgccaggca tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg
    38581 gtttagcgcc aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg
    38641 ggtgcgcatg gtatcgggac aggtagtcag cggcgttgtt gatgcagatg ggaatattct
    38701 tataaacctg taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga
    38761 taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc
    38821 tgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca cccgggcggc
    38881 aaaaacaaca gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat
    38941 gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg
    39001 taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca
    39061 gcaagacttg cagagtaact gatcgtatga cacgtcttgc agagatcctc gaccagatgt
    39121 ccgctgtgct taacgatctc aaaacggtaa tggatcaaga acagcaacat ctctctatgg
    39181 ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcga
    39241 cgctggatta cctcgaacag ttacgcaaga aagaacccaa tacagcaaat agcgttgata
    39301 ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa atgaatcaac
    39361 ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga
    39421 aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg
    39481 gcggtaaaaa gatttcgatc tgaatattat ctccggcctg cactgcaggc cggaatgcat
    39541 tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg
    39601 aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagtaagcg ccagggcatg
    39661 gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc
    39721 agtaccgcca ccaccagacg caacagaaac gccatccagc cgggttgcgg ggtaaagatt
    39781 ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata
    39841 gaaagtgaca gcccggcatg tttcaacgga ccaataaacg ccaggttcat caattgcgtt
    39901 aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag
    39961 ccaggagcca aaactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac
    40021 gcccgctggg tcatcagcgc atcaaaggcg gtaaatttac cgtactggaa cagcgaaacg
    40081 gtcaacggac ctgaaagaat gcccaacgca accgcactcg gcagcgccaa caggaaacaa
    40141 agacgcagcc cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactc
    40201 ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc agacggaaac
    40261 tccattaagc ggtcggcgta atacatccaa gacaccgaac cggaagcaag aaacgaggca
    40321 aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat cgccggtccc
    40381 atctgtttca ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg caggaccagc
    40441 atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggatgcc gccgaccgta
    40501 acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa
    40561 ccaatcatgc tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa gcggttccac
    40621 gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta
    40681 atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg
    40741 gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca
    40801 agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta
    40861 cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc ggcaaagata
    40921 cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg
    40981 ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc
    41041 atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac
    41101 gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg
    41161 aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc
    41221 aacgatacgt tgcgccagca cggcctgttc gccggcggtt tgcggaactg tctgattttg
    41281 cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgttcaa gagtgctctg
    41341 ccaaccagga atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat
    41401 atccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc
    41461 ggcacggcga tgcatacagg tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc
    41521 aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc
    41581 gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata
    41641 aagatcgtgt ggcccgacac tattgctacg atgtttatcc attcttagcg aagctgcggt
    41701 agcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat
    41761 caacgtcagt tttttacgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc
    41821 tgccagcggt ttatcgacac agacatgtac acccgcattg agtaacgtac tgaccacgtc
    41881 aaagtggctg gcggtgctgg aatgcacaaa aaccgcatcg caactggcgg caaggctgga
    41941 taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt
    42001 aggcgaccag gctccttgta acatccagtc agacgctgcc gccagcaccg gtaaccacgc
    42061 tttttgcgca atgccgccta atcccacaac gccgatacgt aattttttca ctttaatctc
    42121 ccagatgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg
    42181 cttcgacgcg ggcctgtaaa tcaccatcaa ccgcgttcga catatccgtt accgccggct
    42241 gatcctctac ctcaccactg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc
    42301 gggccagacg caccacaaaa ggaccatctt cgcgatttgc cagttgttcc agcgtcgact
    42361 ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacctg
    42421 gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttccgct gcgctcagtt
    42481 tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgacc cgattaccaa
    42541 aaccgctcac tgtgcgtaga taatgacgtt tgaccagatt atccagctgt tcctgcactt
    42601 cggattcact cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta
    42661 caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac
    42721 caatcacgcg agcttcaagg gcagtaagtt gatatttcat gagattctcc ttagcggccg
    42781 ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc
    42841 aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg
    42901 ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc
    42961 atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc
    43021 ccaatcgaat aaccgagata gcaggcatga aaagagccac gaacaacatt ggaaaaattg
    43081 gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct
    43141 tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg ataacagtgg
    43201 ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc
    43261 gcaagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag
    43321 cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg
    43381 tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa
    43441 atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg
    43501 ataaaaaaat attgtcccct aagtgatggg aaaagtctta gcggcaatgc agaatagaaa
    43561 gtgctcatct tttatttccc gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg
    43621 ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc
    43681 ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt
    43741 gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc
    43801 gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc
    43861 gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga
    43921 ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca
    43981 caacaacgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt
    44041 ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg
    44101 gccgttttgt ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc atggaaactc
    44161 tccaccgtac gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt
    44221 gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg
    44281 ctgccaatta tggtcaacga cgtggctggc gcaccctctg ccgttaaatg gatgtatgcc
    44341 attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat
    44401 tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg
    44461 gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg
    44521 tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct
    44581 cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat
    44641 atcggtggtg gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg
    44701 atgatgctgg gaattattgg catcttcact ttccttgcgc tgggttggca gtttagtcag
    44761 aaacgtgccg cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc
    44821 ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac
    44881 gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc
    44941 gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa
    45001 caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt
    45061 cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt
    45121 gaagagtggc tgcgcaaggt caatggctac gcaaacaaac tgctgttgcc gcgttttgcc
    45181 aaatcggcat tcgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa
    45241 gaggccagcg cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat
    45301 atcagcgatg atttacgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc
    45361 gaactttcgg aagatgatat tcagctattt ccgctgctgc gtaatctgac gctggtagcc
    45421 ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa
    45481 atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc
    45541 gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc
    45601 gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt
    45661 tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac
    45721 catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat
    45781 cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgacca gccaaattgg
    45841 tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct
    45901 gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa
    45961 agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga
    46021 aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta
    46081 ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc
    46141 gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga
    46201 ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt
    46261 atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc
    46321 atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga
    46381 catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta ttgtaatgcc
    46441 caatctggct ccgcccgtga ccactgttga ggctgccgtg gcgtatcgcc agcgtattct
    46501 tgatgccgta cctgccgggc acgatttcac cccattgatg acctgttatt taacagattc
    46561 gctggatcct aatgagctgg agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact
    46621 ttacccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat
    46681 gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac
    46741 acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc
    46801 tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc
    46861 tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct
    46921 gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc
    46981 catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa
    47041 tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg
    47101 cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga
    47161 agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta
    47221 tgggttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga
    47281 aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg
    47341 gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt aactgtataa
    47401 ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt
    47461 ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt
    47521 atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat
    47581 cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt
    47641 gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg
    47701 ggtcgccccg gcttttttat tttacttaca aattttgagt acaaaatttc tacacttcct
    47761 caaaataatt gcacaatatg ctaaaaaaat atgaatga
//
LOCUS       DAFAVF010000019        42999 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFAVF010000019 DAFAVF010000000
VERSION     DAFAVF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.019"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..318
                     /locus_tag="LGK42_001058"
     CDS             <1..318
                     /locus_tag="LGK42_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBK2657334.1"
                     /translation="NRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGET
                     GTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTM
                     LQL"
     gene            684..1751
                     /locus_tag="LGK42_001059"
     CDS             684..1751
                     /locus_tag="LGK42_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2657335.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1748..4483
                     /gene="rbbA"
                     /locus_tag="LGK42_001060"
     CDS             1748..4483
                     /gene="rbbA"
                     /locus_tag="LGK42_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBK2657336.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4483..5607
                     /locus_tag="LGK42_001061"
     CDS             4483..5607
                     /locus_tag="LGK42_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2657337.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5666..5941
                     /locus_tag="LGK42_001062"
                     /pseudo
     CDS             5666..5941
                     /locus_tag="LGK42_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            5938..6297
                     /locus_tag="LGK42_001063"
     CDS             5938..6297
                     /locus_tag="LGK42_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK2657338.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6417..6818)
                     /gene="nikR"
                     /locus_tag="LGK42_001064"
     CDS             complement(6417..6818)
                     /gene="nikR"
                     /locus_tag="LGK42_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBK2657339.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6824..7630)
                     /gene="nikE"
                     /locus_tag="LGK42_001065"
     CDS             complement(6824..7630)
                     /gene="nikE"
                     /locus_tag="LGK42_001065"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBK2657340.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(7627..8391)
                     /gene="nikD"
                     /locus_tag="LGK42_001066"
     CDS             complement(7627..8391)
                     /gene="nikD"
                     /locus_tag="LGK42_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBK2657341.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8391..9224)
                     /gene="nikC"
                     /locus_tag="LGK42_001067"
     CDS             complement(8391..9224)
                     /gene="nikC"
                     /locus_tag="LGK42_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBK2657342.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9221..10165)
                     /gene="nikB"
                     /locus_tag="LGK42_001068"
     CDS             complement(9221..10165)
                     /gene="nikB"
                     /locus_tag="LGK42_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBK2657343.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10165..11739)
                     /gene="nikA"
                     /locus_tag="LGK42_001069"
     CDS             complement(10165..11739)
                     /gene="nikA"
                     /locus_tag="LGK42_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBK2657344.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11850..12437)
                     /gene="acpT"
                     /locus_tag="LGK42_001070"
     CDS             complement(11850..12437)
                     /gene="acpT"
                     /locus_tag="LGK42_001070"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBK2657345.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12492..13541)
                     /locus_tag="LGK42_001071"
     CDS             complement(12492..13541)
                     /locus_tag="LGK42_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK2657346.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13673..14890
                     /locus_tag="LGK42_001072"
     CDS             13673..14890
                     /locus_tag="LGK42_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657347.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCQGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(14894..15451)
                     /gene="dcrB"
                     /locus_tag="LGK42_001073"
     CDS             complement(14894..15451)
                     /gene="dcrB"
                     /locus_tag="LGK42_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBK2657348.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15524..16189)
                     /locus_tag="LGK42_001074"
     CDS             complement(15524..16189)
                     /locus_tag="LGK42_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBK2657349.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16410..16655
                     /gene="tusA"
                     /locus_tag="LGK42_001075"
     CDS             16410..16655
                     /gene="tusA"
                     /locus_tag="LGK42_001075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBK2657350.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16757..18955)
                     /gene="zntA"
                     /locus_tag="LGK42_001076"
     CDS             complement(16757..18955)
                     /gene="zntA"
                     /locus_tag="LGK42_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBK2657351.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTAVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19029..19655)
                     /locus_tag="LGK42_001077"
     CDS             complement(19029..19655)
                     /locus_tag="LGK42_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBK2657352.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            19796..20155
                     /locus_tag="LGK42_001078"
     CDS             19796..20155
                     /locus_tag="LGK42_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBK2657353.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20158..20427)
                     /locus_tag="LGK42_001079"
     CDS             complement(20158..20427)
                     /locus_tag="LGK42_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBK2657354.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20417..21013)
                     /gene="rsmD"
                     /locus_tag="LGK42_001080"
     CDS             complement(20417..21013)
                     /gene="rsmD"
                     /locus_tag="LGK42_001080"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBK2657355.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21163..22659
                     /gene="ftsY"
                     /locus_tag="LGK42_001081"
     CDS             21163..22659
                     /gene="ftsY"
                     /locus_tag="LGK42_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK2657356.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22662..23330
                     /gene="ftsE"
                     /locus_tag="LGK42_001082"
     CDS             22662..23330
                     /gene="ftsE"
                     /locus_tag="LGK42_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK2657357.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23323..24381
                     /gene="ftsX"
                     /locus_tag="LGK42_001083"
     CDS             23323..24381
                     /gene="ftsX"
                     /locus_tag="LGK42_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBK2657358.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24626..25480
                     /gene="rpoH"
                     /locus_tag="LGK42_001084"
     CDS             24626..25480
                     /gene="rpoH"
                     /locus_tag="LGK42_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBK2657359.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25751..26854
                     /gene="livJ"
                     /locus_tag="LGK42_001085"
     CDS             25751..26854
                     /gene="livJ"
                     /locus_tag="LGK42_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBK2657360.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            27004..27270
                     /locus_tag="LGK42_001086"
     CDS             27004..27270
                     /locus_tag="LGK42_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK2657361.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            27277..27804
                     /locus_tag="LGK42_001087"
     CDS             27277..27804
                     /locus_tag="LGK42_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2657362.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(27801..28184)
                     /gene="panM"
                     /locus_tag="LGK42_001088"
     CDS             complement(27801..28184)
                     /gene="panM"
                     /locus_tag="LGK42_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBK2657363.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            28608..29717
                     /gene="livK"
                     /locus_tag="LGK42_001089"
     CDS             28608..29717
                     /gene="livK"
                     /locus_tag="LGK42_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBK2657364.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29765..30691
                     /gene="livH"
                     /locus_tag="LGK42_001090"
     CDS             29765..30691
                     /gene="livH"
                     /locus_tag="LGK42_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBK2657365.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30688..31965
                     /gene="livM"
                     /locus_tag="LGK42_001091"
     CDS             30688..31965
                     /gene="livM"
                     /locus_tag="LGK42_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBK2657366.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            31962..32729
                     /gene="livG"
                     /locus_tag="LGK42_001092"
     CDS             31962..32729
                     /gene="livG"
                     /locus_tag="LGK42_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBK2657367.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32731..33444
                     /gene="livF"
                     /locus_tag="LGK42_001093"
     CDS             32731..33444
                     /gene="livF"
                     /locus_tag="LGK42_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBK2657368.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGTNGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33840..35156
                     /gene="ugpB"
                     /locus_tag="LGK42_001094"
     CDS             33840..35156
                     /gene="ugpB"
                     /locus_tag="LGK42_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBK2657369.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            35254..36141
                     /gene="ugpA"
                     /locus_tag="LGK42_001095"
     CDS             35254..36141
                     /gene="ugpA"
                     /locus_tag="LGK42_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBK2657370.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            36138..36983
                     /gene="ugpE"
                     /locus_tag="LGK42_001096"
     CDS             36138..36983
                     /gene="ugpE"
                     /locus_tag="LGK42_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBK2657371.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTYLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36985..38055
                     /gene="ugpC"
                     /locus_tag="LGK42_001097"
     CDS             36985..38055
                     /gene="ugpC"
                     /locus_tag="LGK42_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBK2657372.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            38052..38795
                     /gene="ugpQ"
                     /locus_tag="LGK42_001098"
     CDS             38052..38795
                     /gene="ugpQ"
                     /locus_tag="LGK42_001098"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK2657373.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38782..39222)
                     /locus_tag="LGK42_001099"
     CDS             complement(38782..39222)
                     /locus_tag="LGK42_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBK2657374.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            39342..41084
                     /gene="ggt"
                     /locus_tag="LGK42_001100"
     CDS             39342..41084
                     /gene="ggt"
                     /locus_tag="LGK42_001100"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBK2657375.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(41122..41406)
                     /locus_tag="LGK42_001101"
     CDS             complement(41122..41406)
                     /locus_tag="LGK42_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HBK2657376.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(41606..41761)
                     /locus_tag="LGK42_001102"
     CDS             complement(41606..41761)
                     /locus_tag="LGK42_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657377.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(41856..42350)
                     /locus_tag="LGK42_001103"
     CDS             complement(41856..42350)
                     /locus_tag="LGK42_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657378.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            complement(42347..>42999)
                     /locus_tag="LGK42_001104"
     CDS             complement(42347..>42999)
                     /locus_tag="LGK42_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2657379.1"
                     /translation="KAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTA
                     GIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLANL
                     RKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTND
                     FENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAILSGSIYPPMKNIMDHTK"
BASE COUNT        10090 a        10954 c        11880 g        10075 t
ORIGIN      
        1 aatcgttatt tttctgtttc gacagattac agacctaaac ttgaagcact attagcatgc
       61 tcacaaatgt taggtataaa tcaagttgtt gcacataacg gtaatggtgg aagagaatgt
      121 ataggtgaaa cggggacagt tttaggtctt aacgcccgtg acagtaaaca tgctggaaga
      181 atgttcagca tgcataattg ccaaattaac cctggtgcag ggcctgagat aaccactccc
      241 tggaagtctt accagcatga aaaaaacaga aatggactta tgcctttgat tagaaggcgc
      301 acaatgttac agctctgaac catagacaac atgaatatta atgttactat ctggttgaga
      361 ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc gtgaaacgcg
      421 atccaacagg atggataccg ttttgtcagc cagttttata gatcacactt atgagtatgc
      481 gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt tttcactaag
      541 ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta tttgtcgttt ccttcttatc
      601 ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg tcgtttgctt
      661 acagggatta tggcgggccg attatggata agagtaagcg ccatctggcg tggtgggttg
      721 tcggggcact ggcggtggct gccgtcgtgg cgtggtggct gttgcgcccg gcgggtgtgc
      781 cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat atcgccagca
      841 aaattgccgg gcgtatcgac accattctgg tgaaagaagg ccagtttgtt cgcgaaggtg
      901 aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa gccatcgcgc
      961 aaatcaaaga ggcacaaagc gccgttgccg ccgcgcaggc tttgctggag caacgacaaa
     1021 gcgaaactcg cgccgcgcag tcgctggtta atcaacgcca ggcagaactg gactccgtag
     1081 caaaacgtca tatgcgttcc cgttcactgg cccaacgagg ggctatttct gcgcaacagc
     1141 tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg gcaaaagctc
     1201 aggtttctgc ttctaaagcc gctatagagg cggcacgtac caatatcatt caggcgcaaa
     1261 cccgcgtcga agcggcacaa gccactgaac ggcgcattgc cgcagatatc gatgacagcg
     1321 aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca ggggaagtgc
     1381 tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat atgaccttct
     1441 tcctgccaac cgaacaggcg ggcacgctga aactgggtgg tgaagcccgg ctgatcctcg
     1501 atgccgcgcc agatctgcgt attccggcga ccatcagttt tgtcgccagt gtcgcccagt
     1561 tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa actgatgttc cgcgtcaaag
     1621 cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc ggtttaccgg
     1681 gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc gtggtgaggt
     1741 tgccgcaatg acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac tggcgggcgt
     1801 gagccagcat tatggaaaaa ccgttgcgct gaacaatatt actctcgata ttccggcccg
     1861 ctgtatggtc gggctgattg gtccggatgg cgtcgggaag tcgagcttat tgtcgttgat
     1921 ttccggtgcc cgcgtcattg agcagggcaa tgtgatggtg ctgggcggcg atatgcgcga
     1981 cccgaggcat cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg ggctgggcaa
     2041 aaacctctac cacaccttgt cggtgtatga aaacgtcgat tttttcgctc gcctgttcgg
     2101 tcacgacaaa gcggagcggg aagtgcgaat taatgagctg ctgaccagca cggggttagc
     2161 accgtttcgc gatcgtccgg cagggaaact ctccggcggg atgaagcaaa aacttgggct
     2221 gtgctgcgcg ttaatccacg acccggaact gttgatcctt gatgagccaa caacgggggt
     2281 tgacccgctc tcccgcgccc agttctggga tctgatcgac agtattcgtc agcggcagag
     2341 caatatgagc gtgctggtcg ccaccgccta tatggaagag gccgaacgct tcgactggct
     2401 ggtagcgatg aatgccggag aagtgctggc aactggcagc gccgaagagc tacggcagca
     2461 aacgcaaagc gctacgctgg aagaagcatt tataaatctg ttaccgcaag cgcaacgcca
     2521 ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aacgcagaga ttgccatcga
     2581 agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg ttaatttccg
     2641 cattccacgc ggggagattt ttggattcct tggttcgaac ggctgcggta aatccaccac
     2701 catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc tgttcgggca
     2761 accggttgat ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt cgcaggcgtt
     2821 ttcgctctat aacgaactca ccgtgcggca aaaccttgag ttacatgccc gtttgtttca
     2881 catcccggaa gcggaaattc ccgcaagagt ggctgaaatg agcgagcgtt ttaagctcaa
     2941 cgacgttgaa gatattctgc cggagtcatt gccgctcggc attcgccagc ggctttcgct
     3001 ggcggtggcg gtgattcatc gcccggagat gttaatcctc gatgagccta cttctggtgt
     3061 cgatccggtg gcgagggata tgttctggca gttgatggtc gatctctcgc gccaggacaa
     3121 agtgactatc ttcatctcca cccacttcat gaacgaagcg gaacgttgcg accgcatctc
     3181 actgatgcac gccggaaaag tgcttgccag cggtacaccg caggaactgg ttgagaaacg
     3241 cggagccgcc agtctggaag aggcatttat cgcctatttg caggaagcgg caggacagag
     3301 caacgaagcc gaagcgccgc ccgtgataca cgacaccacc cacgcgccgc gtcagggatt
     3361 tagcctgcgc cgtctgttta gctacagccg ccgcgaagca ctggaactgc gacgcgatcc
     3421 ggtacgttcg acgctggcgc tgatgggaac agtgatcctg atgctgataa tgggttacgg
     3481 catcagtatg gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc agaccgtcag
     3541 tagccaggcg tggacgctca atctctccgg ttcccgttac tttatcgaac agccgccgct
     3601 caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatataacgg tggcaattga
     3661 gatccctccc aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac tcggcgtctg
     3721 gatcgacgga gcgatgccga gccgtgccga aacggtaaaa ggttacgtgc aggcaatgca
     3781 ccagagctgg ctacaggatg tggcgagccg acaatccaca cccgccagcc aaagcgggct
     3841 gatgaatatt gagacgcgct atcgctataa cccggacgta aaaagcctgc cagcgattgt
     3901 tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg cccttagcgt
     3961 ggtgcgggaa aaagagcttg ggtcgattat caacctttac gtgaccccca ccacgcgtag
     4021 tgaatttttg cttggtaaac agttgccata catcgcgctg gggatgctga actttttcct
     4081 gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt tcctgacgct
     4141 caccctggcg gcgctgctgt atatcatcat tgccaccgga atggggctgc tgatctccac
     4201 ctttatgaaa agccagattg ccgccatttt cggaacggcg attatcacgt tgatcccggc
     4261 gacacagttt tccgggatga tcgatccggt agcttcgctg gaagggcctg gacgttggat
     4321 cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcggaacgt tctcgaaagc
     4381 gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca tcccgctggt
     4441 gatgggctta agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca tttacgcaat
     4501 atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc gatgctgacg
     4561 ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac gccaggatcg
     4621 ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc gaaccggatc
     4681 gttaacagct tctatcgccc gtggtttttg ccaccggaga tgatcaccgc cgatgagatg
     4741 gatgccggac tggacgccgg acgttatacc tttgcgataa atattccgcc taattttcag
     4801 cgtgatgtcc tcgccggacg ccagccggat attcaggtga acgtcgatgc cacgcgcatg
     4861 agccaggcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga agtgaacagc
     4921 tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac ccggatgcgc
     4981 tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcaatcat caacaacatt
     5041 accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga acacggcacg
     5101 gtggaacact tgttggtgat gccgataacg ccgtttgaga tcatgatggc aaaggtctgg
     5161 tcgatggggc tggtggtgct ggtggtgtcg ggattatcac tggtgctgat ggtgaaaggc
     5221 gtgctgggtg taccgattga aggctcgatc ccgctgttta tgctgggcgt ggcgctcagt
     5281 ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc aatgccgcaa
     5341 ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg tggatccacg
     5401 ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc gacgacacac
     5461 tttgttagcc tcgcgcaggc cattctctac cggggtgccg gattcgaaat cgtctggccg
     5521 cagttcctga cgctgatggc aattggcggc gcgtttttca ccatcgcgct gctgcgattc
     5581 aggaagacga ttgggacaat ggcgtaagga agggaaactt gacttcatac tgtgatgtca
     5641 taaagtgaag tcataaggga atgtcatgta aacacaggta caggctttac gaaagaaaca
     5701 aaaaaacaca ctggatcagc tatttaaatc acctttacct caaggaatca aatggactga
     5761 tatagaatct ctggttaaag cgttaggcgg agaaattaag gaaggtaggg ggtcgcgctg
     5821 taagttcata ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt cgccagatac
     5881 cgataaaggc gctgtagaaa gcgtgcgtga ctggttacta agtatagggg taaaaccatg
     5941 atcaaactga agacgccaaa ctctatggag attgccgggc aacctgctgt tatcacttac
     6001 gtgccagaac tgaatgcatt tcgtggcaaa ttcctgggct tgtcaggtta ttgtgatttt
     6061 gtctctgaca gcattcaggg gctgcaaaaa gaaggggaac tttccctgcg agagtatctt
     6121 gaggattgta aagctgcggg tattgagccg tatgcgcgta cagaaaagat taagaccttt
     6181 acgttacgct atccagaatc gctgagtgaa cgccttaata atgcggctgc gcagcaacaa
     6241 gtctctgtta atacctatat tattgaaaca ctgaatgagc gtttgaatca tttataaatt
     6301 ttagcggcgt acattacgca gaatatctgc ctttacagga tctatctatg caactgaact
     6361 taattttata aattaagttc agttaatatc ttttctttcc tcgtttcaga aataactcaa
     6421 tcttccttcg gcaagcactg caaatgcccg tgccgcacgc cgcgctgggc gataacgtca
     6481 tcggcaaaat gctgcacgtc gcccatatca cctttcagca cggcgatttc cagacagtcg
     6541 tcgtgattga tatgcacatg cagcgtggcg acggagagat cgtggtgatg gtgctgggtg
     6601 gagacaatgc ggctggctaa gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg
     6661 aaaccttgcg tgccgtgctg ctgagtggcc tcctgggcca gggcgctacg cagaatatcg
     6721 cggatagctt cggaacggtt gttataacca cgacgctggc tcaggctgtc cagcgtctcc
     6781 agtaaatcgt catcaagcgt gatggtgact cgttgcattt gcgttaaacc ttttctgtgg
     6841 tgcgacggcg cacggggaat gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag
     6901 aggaaaaggt taatttatct cccaccacct gggtttcgac gatttgtccg ttgtccatca
     6961 ccatcacccg ctgacagaag cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg
     7021 cggtgccaaa ctgttgttgt agctttttca gcaggcgaat gacgcccgcc tgtaacacga
     7081 gatcaaggtt agaaacggct tcatccagaa tcagtagttt cggttcgacc gccagcgcgc
     7141 gagccaggca gacgcgctgg agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa
     7201 cgctgtcatc gagatcgacc gccttcagca tttcgctggc gcgcgccagt tgttcggatt
     7261 ttttcagtga tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg
     7321 gattcacggc gctgatggag tcctgaaata ccatctgaat atcgcggcgg aacgctttac
     7381 gctgggcgcg attgagtttc gccagcggtt cgccgcgcca gctaatattc ccttgtgagg
     7441 gagactctaa gcctaccagc agccgcgcga gggtgctttt tccgcagccg ctgcgtccca
     7501 gcagggcgac ggtttcgccg cttttcaggg tcagggaaac gttattcagc accgcctgat
     7561 gttgatgttt tccgctaaat ccaccgtggg catagtgatg ggaaaggccg cagacgttaa
     7621 gtaaggtcat gatgccagct ccataccgta gagggcgaga tgagcggaaa ccaggctgcg
     7681 cgtcaccgta tgtttggggg cgttaaacag cgtttctaca tcgccctgtt cgacaatttt
     7741 accttgtgac atcaccgcca cgtcatccgc cagacgcgcc acaacgccca tatcatgggt
     7801 gaccagcagc attcccggcg cttgtttttg cataatgctt tccagcagat cgaggatgcg
     7861 cgcctgtgct accacgtcga ggtcggtggt cggttcatcg gcgatgataa acggtgattc
     7921 acacagcacc gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg
     7981 gtacagcttc agcacgcgcg cggcgttttc cagccccacc gcttctatgg cagcggtaag
     8041 cgtagcgtca tcggcgggtt tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat
     8101 ggtgtgcagt ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc
     8161 gcgcagggcg caaggcgaaa ccggtttgcc atcggctaaa atttcccccg ccgtctggcg
     8221 aacgccagcg ggcaaaatgc ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct
     8281 accgccgact aacgccagca cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag
     8341 cggctgcgcg gcctgtagcg cgatattacg tagttcaatc tgttgcggca ttagtgtgcg
     8401 tgctccgtca ccagatgagg atccagatga tcgcgcagtg cgtcacccac caggttaaag
     8461 gccatcacgc tgataaacag cgccagcccc ggccagaaca tttgcagcgg ctgggtccag
     8521 atatactggc gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg
     8581 aggccaagga aagacatccc cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc
     8641 agcaccagca gcgaagggat caccgcgcct gccagatgat cgacaaacac ccgcacatgg
     8701 cccgcaccgg aaagccgtga cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg
     8761 ctgcgcacca tgcgtgcata ccacgcccag tgcgacaggg cgatggcgat aattacgttg
     8821 gtcagcccgg tgccgagcac gccaaccata aagaacgaca gaatcgaggt cgggaaggtc
     8881 ataaacatat cggcgacgcg catggtggcc tgatcaacgc gtccgccaat cagtccggcg
     8941 ctgccgccaa tcaccagccc taatgtcagc accagcagca ggcaggccat taccgaaccg
     9001 agcgacacgc gggtcgccgc catcagccgc gagaaaatat cacgccctaa gtgatcggtg
     9061 cccagccagt gctgcgcatc cggcgaaagc aggcgcgacg gcagatcaat agcctgaggg
     9121 tcatacggca gccaccactg gctggtaagc gcaatcagcg ccagcagggc gataatgatc
     9181 agcgccaggc gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat
     9241 gacgacgaat gcgcgggtcc agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca
     9301 caaaaaccac caccatcatc agcgtaaagc actggatcac cggatagtca cggttaaaaa
     9361 tcgccgacac cgcatagcgc ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg
     9421 tcccgccgat cagttcgccg atgtgcatcc ccacggcggt gatcatcggc agcgaggcat
     9481 tgcgcaggat gtggcgacgt tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg
     9541 tgacgtgacg ctgaccggcg acgtccagca tactggcgcg cagtaaacgc gcgttaatcg
     9601 ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac
     9661 ccatcgcggg tagccattgc agatacaccg aaaacgccat caccagtaaa aacgccagcc
     9721 agaagttagg catcgacacg ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac
     9781 ggtcgcggtg gcgcgccgcc cagataccga gcggtacgga agtgagcaga attaatacca
     9841 gcgccgcacc tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg
     9901 ggcgttggct ggcgaatgag ataccaaagt caagatgcag cgccttccac aaccaggtgc
     9961 cgtactggac gtacagcggc tgatccagtc ccagcatggt gcgggtagag gccagcatct
    10021 ccggcgtcgg cggcaggtta gacagacgca aataatcgag cgccgggtcg ccggtaccga
    10081 ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac catcgggatc agcagcagaa
    10141 agcggcgtaa tacgtaacgc aacattaagg tttcaccggt ttaatctgtt cgaacggaat
    10201 ttcggtggcg atcggcgcgt aggggatgtt acccagctcc ggttttgata ccaccatcat
    10261 cgagatgtag ctgataggca gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg
    10321 atacagcgcc tgacgttgcg tttcgtcatg ggtcgccagc acttcgccga tctctttatc
    10381 aatcagcggc ttgtcggcta atccttgctg tgcctggaag tcagcgtgcg acggtacgcg
    10441 cattgaactc aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat
    10501 gccaaaacga ccgtcgcgct gacgagcata gatactgctc tcttcttcgc caatcagcga
    10561 gacatctgcg ccaatctggc gcatatcagc ctgaatgatt tccgccatcg atttgcttaa
    10621 cgcatcggtg ccgatgaacg acagttcaat gcgcagcggc tgaccatttt tctcgcggat
    10681 gtctttgccc gcaggcagcg tccaaccggc tttttccagc aactctttcg ctttctgcgg
    10741 atcgtactgg cgcggtttca ggccgagatt agcgtaaggc acagaagggg caaacagggt
    10801 gtcggcgacc tgctgggtgc catataacgc gttatcaatc agcgattttt tgtttaccgc
    10861 gtaattaaga gcttcacgta ctgccagctc gttggtgggg gctttggcgg tattgagcgc
    10921 cagcatcacg gtttcgatcg gctgtgacag ttgggtgtgg taagccggat tctggctaaa
    10981 gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc
    11041 agtttcaaac gccaccgcgc gggtagtcgg gtccgggatg acgttaaagg tgatcttttt
    11101 aatcgctggc ttttcgcccc agtagttttc gttacggacg aagacatcgt actgattcag
    11161 tttcgattcc tgcaaaatcc acggtccggt gccaatcggc gctttaattc cgttcatggt
    11221 ttcatggttt ttaaactgcg agggagcgat aaaacggaaa ggacggggca gggccagttc
    11281 ttgcaggaaa ggatagtagg cgcttttcag ggtaatttgc agctctgttt tactgagtgc
    11341 tttaacatca acaatctggt ttgccagctc cagccaggcg tgacgttgac ggttatcgag
    11401 cactgcgcgg aagttttctg ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt
    11461 cacgtcatca cgcagggtga aggtccaggt tttaccatct tctgaatgag tccagctttt
    11521 tgccagccac gggatcaccg aaccgtctgc ctgatatttc accaatggtt cataaaccat
    11581 gctctgggcg aacatctggt taggcgtgta aaggtgcggg tttagtggcc cgacattcac
    11641 cggccaggcg gtggtgattt catctggtgc agcggcatgg acgataaaag acgcacaagc
    11701 cagcagcgca aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa
    11761 taggtggcgg aaataagtat gacgatttta agtattcgtc atactgatta cctgttctgg
    11821 atcaatagat gggcagtcgg gtgggcggat cagttaactg aatcgatcca ttgcacactg
    11881 tcggcggtga gcgtaaaggg ggtgggggtg cagatcgcca ggcttaaatt ttcaagctga
    11941 caatggctga ctgacagcga ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg
    12001 ctgccgccac gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc
    12061 agttgctgat caggatgcac ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg
    12121 ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg
    12181 ccttcatcac tcaacagcag agcgatatcg tcaccgctat ggcttaagtt gaaccatagc
    12241 ggcgtttccg gcgcgaatgc aggtttgcct tgttcgccat agatgatctc cggtagcggg
    12301 gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc
    12361 ggtgcttgct cgcgtaaacc cggtggcagt ggagctgcgc ttaaggtcga aactttcccc
    12421 agaactatcc gatacatatc agggccaacg tttaatggaa aatgaaagtg cgtatcgtat
    12481 cacttgtcgc ctcatcccgg taaccgactt ttcggtctgc ccggccccag taaaatcgcc
    12541 agtttgctgc cacctttcgt cgtttccatc cagattttac acacgctggt taatggcacc
    12601 gaaagtagca tccctaccgg gccgagcagc catccccaga ttaacaatga aagaaatacc
    12661 accatggtgg acatccccag gcgatggccc atcatccgtg gctctaaaat attgccgatg
    12721 accatatgga caactaaaaa caatgcgccg accagaatac attcgtaaat accattaaac
    12781 agcagcacct gaatcattgg cggtacggcg gaaattaccg cgccgatatt gggcacgtag
    12841 ttaagcaaaa acgccagtac tgcccacatc agcgcaaact gtacccccat cagctccagc
    12901 cccagccaga cgatgacacc tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc
    12961 gaaacgcctt taagtgcgcg gtgtaatccc gcgatgtgaa tctgtggatt attcagcgca
    13021 aaacgcattt tgtaagggac gtggcgcact tcaaacagca taaaaactac ggtcatcacc
    13081 agcaaaagca cgctcgccat tgccccggaa agcccggtca ttagcgctgt ggtgaaggta
    13141 accacttttt ccgagtccat ccgctgcagc attcgctccg gcgacatatg caaattaaga
    13201 aaaggcaaca tctcctgcaa tttaaaaagt ttgcgcgtca gctccttatt aaacttcggc
    13261 agcatagaga taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc ggttagtgtg
    13321 atcagcatca ccaccaccac aatcgtaatg gcaacggggc gttttactcc ccgtcgaata
    13381 aaccaggtaa cgagcgggtt gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca
    13441 atgatatctg ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc cagcttgagc
    13501 agaatgtgca tgcccgtttt atcgggttga ggggtttcca ttggggcttc cttgtgactt
    13561 tttgtattaa gtgtagcggg agtcacgcca gcaatattct gattcttact gctgattttc
    13621 acgtcagcac atggtaaaaa tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga
    13681 acccgtagcc gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg tctccattgt
    13741 catgtttaac ttcgccagct acctcaccat cgggttgccg ctcgctgtat taccgggcta
    13801 tgtccatgat gtgatgggct ttagcgcttt ttgggcagga ttggttatca gcctgcaata
    13861 cttcgccacc ttgctgagcc gccctcatgc cggacgttac gccgatttgc tgggacccaa
    13921 aaagattgtc gtcttcggtt tatgcggctg ctttttgagc ggtctgggat atctgacggc
    13981 aggattaacc gccagtctgc ccgtcatcag cctgttatta ctttgccagg gacgcgtgat
    14041 ccttgggatt gggcaaagtt ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt
    14101 tggctcgctg catatcgggc gggtgatttc gtggaacggc attgtcactt atggggcgat
    14161 ggcgatgggt gcgccgttag gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc
    14221 gttaatcatt atgggcgtgg cgctggtggc cattttgttg gcgatcccgc gtccgacggt
    14281 aaaagccagt aaaggcaaac cgctgccgtt tcgcgcggtg cttgggagcg tctggctgta
    14341 cggtatggcg ctggcactgg cttccgccgg atttggcgtc atcgccacct ttatcacgct
    14401 gttttatgac gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc
    14461 gtttgtcggt acgcgtttgt tattccctaa cggcattaac cgtatcggtg gcttaaacgt
    14521 agcgatgatt tgctttagcg ttgagataat cggcctgcta ctggttggcg tggcgactat
    14581 gccgtggatg gcgaaaatcg gcgtcttact ggcgggggcc gggttttcgc tggtgttccc
    14641 ggcattgggt gtagtggcgg taaaagcggt tccgcagcaa aatcaggggg cggcgctggc
    14701 aacttacacc gtatttatgg atttatcgct tggcgtgact ggaccactgg ctgggctggt
    14761 gatgagctgg gcgggcgtac cggtgattta tctggcggcg gcgggactgg tcgcaatcgc
    14821 gttattactg acgtggcgat taaaaaaacg gcctccggaa cacgtccctg aggccgcctc
    14881 atcatcttaa aacttactga ataaccagcg tattaatgat gttttctgcg gtggtctgcg
    14941 ctttttgctg atcgtcagcg ggcagcgtaa tttgcatggt cagcagttga ttacccacgt
    15001 tacccagaat aacggaagag tacgccgtct ggcctttcgc ggagataata ctgtctaact
    15061 gctgcatttt gtgacctttc agctcaatgg ctttattggt taccacttgc agctgcggat
    15121 cgcggctacg ttgctgatct tccagacgct tcgccagcac cgccagatct tctttcggat
    15181 catcgcccat gatgacgatg actgctttct gcccggtggc gtcggaccag acatgcatgt
    15241 tattggcctg cgttcccagc ttaccgctct ggtcggtcat atccgctggc agcgagaaac
    15301 ttaacttgcc atcaagcagg ttgacgggat tcccggtagc gttactttcc gcgaccgaac
    15361 cctgcgccgt agcgttagtg tctttatcat cacaggccgc aagccccata accagcaggc
    15421 caattccgac atatttaacc agattgcgca ttgacttctt cctttcgata aacggccata
    15481 acggctcatt catccatctt atcacaactc tgataacgaa cctttaactc gcctgcaaag
    15541 cgttgatttc ggatttatct gccagtcttt tcaacagcat attgagtaat acgccataca
    15601 ttggcaggaa gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt
    15661 ccatccagtg ttcagccata aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg
    15721 ccagcgtgtc actgacgtta ccgaacagtg tggacgctgt cggtgccagc caccagcggc
    15781 gactttgacg caggcggtta aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca
    15841 tgaaactggc ggtggcgata cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc
    15901 cctgccatga acccatatag aacagcgacg agatgacata ggagattaat aacgcaggga
    15961 tcattaccgc gaagataatg cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt
    16021 cggtcgcgag aaagataaac ggaaaactaa acgcgcccca ggtggtatga aaaccaaaaa
    16081 tggagaccgg aagctggacc agatagttac tggaggtgat caccagcaga tgaaataacg
    16141 ataaccagaa caacgcctta tagcgttgag tttgcgagaa aacgttcata ttgtaccttt
    16201 ttgattaacc attggggtga gggaacccaa tacgtacgac acgtctgtta tcttaacata
    16261 ataacgtgct tacccttttt tcgagccgcc gcatgatact gctttacgtt gacaatgcaa
    16321 tggttaattt tcacgcaatc gttaacctgg tttgcttacg gactcacagg gcgtaaacta
    16381 gcgccgtttt tttaagtgat gagaagaaaa tgaccgatct cttttccagc cctgaccaca
    16441 cactcgacgc gcttggcctg cgctgcccgg aaccggtgat gatggtgcgc aaaaccgtgc
    16501 gcaatatgca gcctggcgaa actttactga ttatcgccga cgatccggcg acgacccgcg
    16561 atattcctgg gttttgtacc tttatggaac acgaactggt tgcgaaagag acggatggac
    16621 tgccttatcg ttatttgatt cgtaaaggcg gttgatgggg aaagattggc ttcgatgccg
    16681 ccttttcccc tcaccctaac cctctcccca gaggagcgag gggaccgatc gcgctcaatg
    16741 ttgcgatcgg tttgccttat ctcctgcgca acaatcttaa cgcattcgct gttaccagca
    16801 cggtcgcacc ggtatctgcc agcaccgcca gccatagtcc ggtcatccct agcagcgtgg
    16861 tgacgaggaa gatccctttc agccccagcg caatagtgat gttctggcgg atattggcgt
    16921 gagtggcgcg tgccagttca atcatttgca ccaggccgcg caggtggtta tgggttaatg
    16981 ctgcgtcggc ggtttccagc gccacgtctg tgccactgcc cattgcaatc ccaatggcgg
    17041 cagctttcat cgctggcgcg tcgttaatac catcaccgac catcgccagg ggcgcatgtt
    17101 gattcagctt ggtcaccgct ttgactttat cttccggcaa taggcccgct ttaaactcca
    17161 gccccagctc cccggcaatt gccgccgctg cgcgtggatt atcgccggtg aggatcactc
    17221 ctttaacccc cagcgcgttc agttcactga tggcagttgc tgcgtcggcg cgcagggtat
    17281 cctgcaatgc aatgataccc agcacgtcat cattacgtac taccagcaca accgtttgcc
    17341 cggcgctttc cagttcatta atcaaaccag caaatgcatc agcgggatgt ttcccggcag
    17401 cgcaaatcaa tacgcgctca ccgttaacct gcgcttcaat gccagacccg accagcgccc
    17461 gctgtgattc ggcggtggga atggcgagtt cagcaacctg tgcttcgcgt acgatggcct
    17521 gcgccagtgg atgcgtcgcg ccttgctcga ccgccgccgc cagtgtcagc agttcagatt
    17581 cactaatacc cgttgccgga tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac
    17641 cggttttatc aaacgccacc tgagtaacac gacccagctg ttccagcgcc gctccgcctt
    17701 taatcaacgc cccacgacgt gccgctgctg ccagcccgga ggtaatcgcc gcgggcgtgg
    17761 agataactaa cgcacacggg cagccaatca gcagcagcgt caggccttta taaatccact
    17821 cttgccagct ggcggcaaac agcagcggcg gcaccagcgt taccagcagg gcgacggcca
    17881 taattgctgg cgtataaatg cggctgaaac ggtcgataaa ccgctcaatg ggggcgcggc
    17941 gctcttcggc ttcttcgatc agtttcagaa tccggtcaat ggcgctggca cccggttctg
    18001 acagcacttc cagcgttacc agacggtcta cgctggtggc tccagcaggg actttatcgc
    18061 ccgtcgcgcg ctccaccgga atggattctc cggtcagggc gctttcatca aaactggcaa
    18121 acggtgagag caatttaccg tcggcaggca aacgcccacc tgcggcgact tcaatcacat
    18181 cgccagggcg caggctgtta atcgccacct cttcccgctc accgttacgc aggcgcgtgg
    18241 cggtttctgg tttcagcgcc attaacgcgc tgaccccttg acgtgcgcgg ctggcggccc
    18301 agccttccag tcgttcgcca atcaaaaaca gcagcaacac catcgcagct tcagccgttg
    18361 cgccaataaa cagcgcacca atagcggcta cgctcattaa cgtttcaatg gcgaagtagc
    18421 tgccggattt gatcaaccgt aatgcctgac gagcaatcgg gtacagccca accagcgtgg
    18481 tcgcgataaa cgccagttgc ccgaacggat gattgaactg ctccagaccc cagctgattg
    18541 ccatcatcac gattagcgta atcagcgaca gattctcttt caggcgtgat gcttgcggtt
    18601 cttcggcggc ctgttcatcg cgcagggaat agcctgcttt ttgcaccgca gattcaactt
    18661 gtgcacgaat gtcattgtcg gcatcgacca ccagtttttc ggtggcgaac aacacctgca
    18721 cctgattcac gcctgcaagc tggcgcacag cattttctac cttgcgcgca caggcggcgc
    18781 agtccatgcc gctgactttc cagctatagc gggtgccgga gacgttttca gagagcgttg
    18841 gcgagctgga acatgcgccg tcgcagcaac agtcgttggc gttctgtacc gcagttagcg
    18901 gtttgaacgc agcaaattga ggggctttct tgccgtgatt gtcaggagtc gacatggcat
    18961 cctccggtta agttttttct cattaaccga aggatacact ctggagtcga ctccagagtc
    19021 aagttttatc agagatacag cgagcggacg atgaggaagt gaccggcaaa gtagcaggcc
    19081 gcagcaatcg cgttatccgc acggaagcgt cggcgatagt gactacccag ccagacaaag
    19141 ttactgataa acagcaacga agcgccgaca aacgcagaga gcgccggagc ggtcggacgg
    19201 aagaaccaca gttcacctgc cagccacacc atcaccagcg tcatgccgat aaacgtgcag
    19261 ataggccaac gatactcttc cagccgcgtc cagataatcg ccagtaacag cgcgcccaga
    19321 accagcagca ccagtggcag aggccagaag aaagagagcg tcatctgact ggcgaaatag
    19381 atggtgtaca gcaggtgcga gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt
    19441 ggcaacaggg ttagcgcatc gcccagcagt gaggcgcaca gccccgccag caccagatag
    19501 ctaatggcgt cgaacatcgg cgcttgccag gccagtaaca gcaggagaag aagggttaat
    19561 ggtttaaata cccagcgttg ccaggttggc ccacgatacg atgcatccac agatagccat
    19621 gcggaaagac agacagcgat aaacgaccaa agcatcttag ttccttgaat tcgttatttt
    19681 ttcgtaagcg gaatgcctac ggtcttgctt ccagtttagt ggcgagggtg gacggatgac
    19741 aacaccataa ctggcagggt atgcttaatt ccgcattttc cgatgaggga taaagatgag
    19801 caaaccacct cttttcttta ttgttatcat tggcttaatc atcgtcgccg catcgtttcg
    19861 ttttatgcaa caacgacggg aaaaagctga taatgatatg gcaccactcc agcaaaagct
    19921 ggtgttggtg agcaacaagc gggaaaaacc gattaacgat cgccgttcgc gccagcagga
    19981 agtgactccg gcaggcacca gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg
    20041 aatggagcag acgtttcgcc tcgacgccca gcagtaccac gccctgacag tgggcgataa
    20101 aggtacgctg agctataaag gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta
    20161 ttttttaact ttaaatttct tctgccagac cagcagttca aacacgccga aaaggaaaat
    20221 ccgtaccttt tcggcggtgg tcatctgcgg gccatctttc ggtaaagtgg atttcaatag
    20281 cgccagctgc ataccatgca tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt
    20341 cagcggacgt gggaagggat gcaccaggtt gaggattaaa aatccccaaa cacagagcat
    20401 taacaaacga ccaatattaa tcagcatcac tttctccttg tgcttcgcgt tgatacagcc
    20461 gataagccac ctgacccgcc actttttccc gatgtaatga ccagtttgcc ggaacagtgg
    20521 gcagaccgtt ttcgacttcg ctttcgacat aaatcagggc ttcgtcagcc agccagccgt
    20581 tatcttccag taaatttatc gtctcttcta acaagccacg gcggaacggt ggatcgacaa
    20641 acacgatatt atgcggtgtg cctttttgcg ccaggaatga cattgcgttg ctgttcacca
    20701 cgcgtgcatt gcctgctttt agtgtcgcca gattcttaat taactgctga gaaaccgcgc
    20761 gatccatctc aatcaacgtg gcccccgcag catagcgcga caacgcttcc agccccagcg
    20821 cgccgctccc ggcgaagcaa tccagacatt gggcgtcaac aatgaccgga gccagccagt
    20881 taaacaacgt ttcgcgtacg cggtcggtgg tggggcgcag acctgggcta tcaggaaccg
    20941 ggagtttacg gcctcgccac tgcccgccaa taatgcggat ttggccgctg ccggaatgat
    21001 tcggtttttt catgataaat tgctcaatcc gccagataac acatacttgc aggcggtgat
    21061 gtgaattaag ttaagtgata gactatttca tcattttttt agctgctatg tacatagcgt
    21121 taacgctgtg ccatgaagca acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa
    21181 cgtggctttt tttcctggct gggctttggt caaaaagagc agaccccgga aaaagagaca
    21241 gaagttcaga atgaacaaac ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag
    21301 gcttctgaac aagccgtcga agagcagccg caggcgcata ctgaggccga ggcggaaact
    21361 tttgctgccg acgttgtgga agtcactgaa caggttgctg aaagtgaaaa agcgcagcct
    21421 gaagcggaag tcgttgcaca accggaaccg gtcgtagaag aaacgccgga gccagtggct
    21481 atcgaacgtg aagagctgcc gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa
    21541 gagtggcagg ctgaagcgga aaccgtagag attgtcgaag cggcggaaga agaagcggct
    21601 aaagaagaaa tcaccgacga agagctggaa gcacaggcgc tggctgccga agcggcagaa
    21661 gaagcggtga tagtggttcc tccggcagaa gaagagcagc cggttgaaga aattgcccag
    21721 gagcaggaaa aaccgaccaa agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa
    21781 accaaagaaa atctcggttc cggatttatc agcctgttcc gcggtaaaaa aatcgacgat
    21841 gatctgtttg aagagctgga agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca
    21901 cgtaaaatca tcaccaatct gacggaaggc gcatcccgca agcagcttcg tgatgccgag
    21961 gccctctatg gcctgctgaa agaagagatg ggcgagattc tggcgaaagt cgatgagccg
    22021 ctgaatgttg aaggtaaaac gccgttcgtg atcctgatgg taggcgtcaa cggtgtgggt
    22081 aaaaccacga cgattggtaa gctggcgcgt cagtttgagc agcagggtaa atcggtgatg
    22141 ctggcggcgg gcgatacttt ccgtgcagca gcggttgaac agcttcaggt ctgggggcag
    22201 cgcaacaata ttccggtgat tgcccagcat accggtgcgg attccgcctc tgttatcttc
    22261 gacgccattc aggccgctaa agcgcgtaat atcgacgtcc tgattgccga taccgccgga
    22321 cgcctgcaga acaaatcgca cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag
    22381 aaactcgacg ttgaagcgcc gcatgaagtt atgctgacta tcgatgccag caccgggcag
    22441 aacgcggtaa gccaggccaa actgttccat gaagctgttg gcttaaccgg catcacgcta
    22501 acgaaactgg acggcacggc gaaaggcggg gtaattttct cggtggctga ccagtttggt
    22561 atccctatcc gctacattgg tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg
    22621 gacgacttta tagaggcact ttttgcccga gaggattaac aatgattcgc tttgaacatg
    22681 tcagcaaggc ttatctcggt gggagacagg cgctgcaggg cgttacgttc catatgcagc
    22741 cgggtgagat ggcgtttctg accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc
    22801 tgatctgtgg gattgagcgg cccagcgccg ggaaaatctg gtttagcggc catgacatca
    22861 cgcgtctgaa aaaccgtgaa gttccgtttc tgcgccgcca gattggcatg attttccagg
    22921 atcaccatct actgatggac cgtactgtct acgataacgt ggcaatcccg ctgattatcg
    22981 ccggtgccag cggtgacgat attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc
    23041 tactggacaa agcgaagaac ttccctattc agctttctgg cggtgaacaa cagcgtgttg
    23101 gcattgcccg cgcggtggtg aacaagcccg cggtactgct ggcggacgaa ccgactggta
    23161 acctggacga cgcgttgtcg gaagggattc tacgtctgtt tgaagagttt aaccgcgttg
    23221 gggtaaccgt attgatggca acgcacgaca tcaacctgat ctcgcggcgt tcctatcgca
    23281 tgctcaccct gagcgatggt cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca
    23341 atcaatcata ttcggcagtt tggcgggcgt ctggatcgct tccgtaaatc ggtcggcggc
    23401 tctggcgacg gcggtcgtaa cgcgccaaaa cgcgcgaaat cctcgccaaa accggtaaat
    23461 cgcaaaacca acgttttcaa cgaacaggtg cgctatgcct tccacggcgc attgcaggat
    23521 ctgaaaagca aaccgttcgc cacgttttta acggtgatgg ttatcgccat ctccctgacg
    23581 ctgcccagcg tctgttatat ggtgtacaaa aacgttaacc aggcggcgac gcaatattat
    23641 ccgtcaccgc aaatcactgt ttacttgcaa aaaacgctgg acgatgacgc cgcagcaggc
    23701 gtggtagcac agctacaggc cgagcagggc gtggagaaag tgaactatct ttctcgtgaa
    23761 gacgcactgg gcgagttccg taactggtct ggttttggtg gtgcgctgga tatgctggaa
    23821 gaaaacccgc ttccggcagt ggcggtggtg atcccgaaac tcgattttca ggggacggaa
    23881 tcactgaata cgctgcgtga tcgtatcacc cagattaacg gcattgacga agtgcggatg
    23941 gatgacagct ggtttgcccg tctggcggcg ttgaccgggc tggtcgggcg cgtttccgcg
    24001 atgatcggcg tgttgatggt ggcagcagtg ttcctcgtca tcggcaacag tgtgcgtctg
    24061 agcatctttg ctcgccgtga ctccattaac gtgcagaaac tgattggtgc gacggatggt
    24121 ttcatcctgc gcccgttcct gtacggaggc gcattgctgg gattttctgg cgcattgctg
    24181 tcattaattt tgtcagaaat tctggtactg cgtttgtcat cggcggttgc agaagtggca
    24241 caggttttcg gaacgaagtt tgatatcaat ggcttatcgt tcgatgaatg cctgctgttg
    24301 ctgctggttt gctctatgat tggctgggtg gcagcgtggc ttgccacggt acaacattta
    24361 cgccacttta cgcctgaata ataaaagcgt gttatactct ttccctgcaa tgggttccgt
    24421 agcagggaaa gagaccccgt tgtctcttcc cggtatttca tctctatgtc acattttgtg
    24481 cgtaatttat tcacaagctt gcattgaact tgtggataaa atcacggtct gataaaacag
    24541 tgaatgataa cctcgttgct cttaagctct ggcacagttg ttgctaccac tgaagcgcca
    24601 gaagatatcg attgagagga tttgaatgac tgacaaaatg caaagtttag ctttagcccc
    24661 agttggcaac ctggattcct acatccgggc agctaacgcg tggccgatgt tgtcggctga
    24721 cgaggagcgg gcgctggctg aaaagctgca ttaccatggc gatctggaag cagctaaaac
    24781 gctgatcctg tctcacctgc ggtttgttgt tcatattgct cgtaattatg cgggctatgg
    24841 cctgccacag gcggatttga ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg
    24901 tttcaacccg gaagtgggtg tgcgcctggt ctccttcgcc gtccactgga tcaaagcaga
    24961 gatccacgaa tacgttctgc gtaactggcg tatcgtcaaa gttgcgacta ccaaagcgca
    25021 gcgcaaactg ttcttcaacc tgcgtaaaac taagcagcgt ctgggctggt ttaaccagga
    25081 tgaagtcgaa atggtggccc gtgaactggg cgtaaccagc aaagacgttc gtgagatgga
    25141 atcacgtatg gcggcacagg acatgacctt tgacctgtct tccgacgacg attccgacag
    25201 ccagccgatg gcaccggtgc tctatctgca ggataaatca tctaactttg ccgacggcat
    25261 cgaagatgat aactgggaag agcaggcggc aaaccgtctg accgacgcga tgcagggtct
    25321 ggacgaacgc agccaggaca tcatccgtgc gcgctggctg gacgaagaca acaagtccac
    25381 gttgcaggaa ctggctgacc gttacggtgt ttccgccgag cgtgtgcgcc agctggaaaa
    25441 gaacgcgatg aaaaaacttc gcgctgccat tgaagcgtaa tttctactac taagcagaga
    25501 accctggatg agaatccggg gtttttgttt tttgggcctc tacaataatc aattccccct
    25561 ccggcaaaac gtcaatcccc acgcagattg ttaataaact gtcaaaatag ctattccaat
    25621 atcataaaaa tggggtatgt tttagcagag tatgctgcta aagcacgggt agtcatgcat
    25681 aaaacgaaat aaagtgctga atcacaacac cacaacacac gtaataacca gaagaatggg
    25741 gattctcagg atgaacataa agggtaaagc gttactggca ggatgtatcg cgctggcatt
    25801 cagcaatatg gctctggcag aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc
    25861 ggttgcgcag tacggtgacc aggagtttac cggcgcagag caggcggttg cggatatcaa
    25921 cgctaaaggc ggcattaaag gcaacaaact gcaaatcgta aaatatgacg atgcctgtga
    25981 cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac gacggcatta aatatgtgat
    26041 tggtcacctc tgttcttcat caacgcagcc tgcgtctgac atctacgaag acgaaggcat
    26101 tttaatgatc accccagcgg caaccgcgcc ggagctgacc gcccgtggct atcagctgat
    26161 cctgcgcacc accggcctgg actccgacca ggggccgacg gcggcgaaat atattcttga
    26221 gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct
    26281 ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg
    26341 tatcaccgcc ggggaaaaag atttctcaac gctggtggcg cgtctgaaaa aagagaatat
    26401 cgacttcgtt tactacggcg gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg
    26461 cgcggcaggg ctgaaaactc agtttatggg gccggaaggt gtggctaacg tttcgctgtc
    26521 taacattgcg ggcgaatcag cggaagggct gctggtgacc aagccgaaga actacgatca
    26581 ggttccggcg aacaaaccca ttgttgacgc catcaaagcg aaaaaacagg atccaagtgg
    26641 cgcgttcgtc tggaccacct acgccgcgct gcaatctttg caggcgggcc tgaatcagtc
    26701 tgacgatccg gctgaaatcg ccaaatacct gaaagcgaac tcggttgata ccgtgatggg
    26761 gccgctgacg tgggatgaga aaggcgatct gaaaggcttt gagttcggcg tatttgactg
    26821 gcacgccaac ggcacggcaa ccgatgcgaa gtaacagata gataaatgaa aatgcccgat
    26881 gcgctgaact gccatcgggt ttttttgtgt ttgtcgaaat ggttgtagaa agattaaaca
    26941 atccaggata tgcttaatgt acggtgacat accgtacata ttatacggag caggagagtt
    27001 cacatgtctg ctgttaaaaa gcagcgtatc gatctgcgtt taactgacga tgacaaaagt
    27061 atgattgagg aagcggcagc gatatctaat cagtccgtca gccaatttat gttgaacagc
    27121 gcttcgcagc gggctgcgga agtgattgaa cagcatcggc gggtgatcct caatgaggaa
    27181 tcctggacgc gggtgatgga tgcgctgagt aatccaccgt cacctggtga aaagctaaaa
    27241 cgtgcggcaa aacgtcttca gggaatgtaa taatttatgg atgatctgac gatagagatt
    27301 ctgaccgatg atgcagatta tgatctacag cgattcgact gcggcgagga agcgttaaat
    27361 ctctttctga cgacacatct cgttcgtcaa catcgcaaca aaattctgcg agcgtatatc
    27421 ctttgtcgca acactccaga acgtcaggtg ctgggatatt acacattatg cggcagttgt
    27481 tttgaacgag ccgcattgcc ctcgaaatcg aaacagaaaa aaattcccta caaaaatatt
    27541 cccagcgtta ctcttgggcg tctggcaatt gatcgttcat tacaggggca gggatgggga
    27601 gcaacactgg ttgctcatgc catgaaagtc gtctggtcag cctctttagc ggtaggtatt
    27661 cacggtcttt ttgtcgaggc gctgaataaa aaagcccata cgttttatca atcgctgggc
    27721 tttatcccgt tggtcggaga aaacgaaaat gcgttatttt tcccaaccaa atccatcgaa
    27781 ctgcttttca cacagagcga ttaacgcttc tcccagccgc cctgttgcgc cgtaaatccc
    27841 agtgcctgca taaacgccgt catcacaccg cgatcttcca cgcctgcatc cgccatccac
    27901 cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg ccccacaccg
    27961 cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt gccgcttaag
    28021 gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat acggtggtta
    28081 tcgtcaacct gtaaagagga aggggaatac tccggccaga ttttttgcag gtcaatccgg
    28141 tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa cccgtgtaaa
    28201 tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc ttttttattt
    28261 cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac attagagaat
    28321 gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt tgtgccgtta
    28381 ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag aatattatct
    28441 gttcttaatc gactgaaaaa tagggatttt aatcactatt atcacaaaat actgcgctaa
    28501 acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct gatttgttaa
    28561 cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg aaacggaatg
    28621 cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct atggctgacg
    28681 atattaaagt tgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg
    28741 aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga attaaaggcg
    28801 ataagctggt gggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg
    28861 ccaacaaaat cgttaacgac ggcattaaat acgttattgg tcatctgtgt tcttcttcta
    28921 cccaacctgc gtcagatatc tacgaagacg aaggtattct gatgatctcg ccgggagcga
    28981 ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc gggctggact
    29041 cttcccaggg gcctacggcg gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg
    29101 ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg caggacgggc
    29161 taaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg gagaaagatt
    29221 tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt
    29281 actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg aaaactcagt
    29341 ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgccggt gatgctgccg
    29401 aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac cagggcatcg
    29461 ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg atcacctacg
    29521 cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag ccgctggcgc
    29581 tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg aactgggatg
    29641 aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc gacggttcat
    29701 ccacggcagc caagtgatca tcccaccgcc cgtaaaatgc gggcgggttt agaaaggtta
    29761 ccttatgtct gagcagtttt tgtatttctt gcagcagatg tttaacggcg tcacgctggg
    29821 cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta tcggcatgat
    29881 caacttcgcc cacggcgagg tttatatgat tggcagctac gtctcattta tgatcatcgc
    29941 cgcgctgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg
    30001 cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct atcgcccggt
    30061 gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca tcttcctgca
    30121 aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc tgtttaacgg
    30181 gcagtgggtg gtgggccata gcgaaaactt ctctgcctct attactacca tgcaggcggt
    30241 gatttggatt gttaccttcc tcgccatgct ggcgctgacg attttcattc gctattcccg
    30301 tatggggcgc gcctgtcgtg cctgcgcgga agatctgaaa atggcgagcc tgcttggcat
    30361 caacaccgac cgggtgattg cgctgacctt tgtgattggc gcggcgatgg cggcggtggc
    30421 gggtgtgctg ctcggtcagt tctacggtgt gattaacccc tacatcggct ttatggccgg
    30481 gatgaaagcc tttaccgcgg cggtgctcgg cgggattggc agcattccgg gggcgatgat
    30541 tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga gtacggaata
    30601 taaagatgtg gtctcatttg ccctgctgat cctggtactg ctggtgatgc cgaccggtat
    30661 tctggggcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat ggcgctgctc
    30721 tctgccgcga tgttctttat cctcgctggc gtctttatgg gcgtacagct ggagctggac
    30781 ggcaccaaac tggtggtcga cacggcttcg gatgtccgtt ggcagtgggt gtttatcggc
    30841 acggcggtgg tctttttctt ccagattttg cgacctgctt tccagaaagg gttgaaaagc
    30901 gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa gcagaaactg
    30961 ttcctcgtgg cgctgttggt gcttgcggtg gcgtggccgt ttatggtttc acgcgggacg
    31021 gtggatatcg ccaccctgac catgatctac attatcctcg gcctcgggct gaacgtggtg
    31081 gtagggcttt ccggcctgct ggtgctgggg tacggcggtt tttacgccat cggcgcttac
    31141 acttttgcgc tgctcaatca ctattacggc ttgggcttct ggacctgtct gccgattgcc
    31201 gggttaatgg cagcggcggc gggcttcctg ctcggctttc cggtgctgcg tctgcgtggt
    31261 gattatctgg cgatcgttac cctcggcttc ggcgaaattg tgcgcatatt gctgctcaat
    31321 aacaccgaaa ttaccggcgg cccgaacgga atcagtcaga tcccgaaacc gactttcttc
    31381 ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag taatttcttt
    31441 ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc gttgctgctg
    31501 gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg
    31561 gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc
    31621 aagctgaccg cttttaccat cagtgccgcg tttgccggtt ttgccggaac gctgtttgct
    31681 gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc gtttgtactg
    31741 gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc ggcaattttg
    31801 ctggtggtgt cgcgcgagtt gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt
    31861 ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat gacgcgcccg
    31921 caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag ccattattat
    31981 ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac gtcaatcttg
    32041 aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg
    32101 tttttaactg cctgaccggt ttctacaaac ccaccggcgg caccatttta ctgcgcgatc
    32161 agcatcttga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg cgcaccttcc
    32221 agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg gcgcagcatc
    32281 agcaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc
    32341 agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg ctggaacacg
    32401 ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag attgcccgct
    32461 gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt cttaacccaa
    32521 aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac aacaccacta
    32581 tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga atttatgtgg
    32641 tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat aacccggacg
    32701 tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc ctttgacaaa
    32761 gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca tatcaatcag
    32821 ggcgagattg tcaccctcat tggcacgaac ggggcgggga aaaccacctt gctcggcacg
    32881 ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa agacattacc
    32941 gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc
    33001 gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt tgccgaacgc
    33061 gaccagttcc aggagcgcat aaagtgggtg tatgaactgt ttccacgtct gcatgagcgc
    33121 cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc gattggtcgc
    33181 gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgctcgg tcttgcgccg
    33241 attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg gatgactatc
    33301 ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg
    33361 gaaaacggcc atgtggtgct ctccgatacc ggcgacgcgc tgctggcgaa cgaagcagtg
    33421 agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa aagactcctt
    33481 tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc ctactgggtg
    33541 agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt acgccgcatc
    33601 cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt tccgcttatc
    33661 cgtgccccat ctcccatttt ccctcaccca cgccgtcacc gccttgtcat ctttctgaca
    33721 ccttactatc ttacaaatgt aacaaaaaag ttatttttct gtaattcgag catgtcatgt
    33781 taccccgcga gcataaaacg cgtgaattcg cgcattcggt acaacaagag agataaacga
    33841 tgaaaccgtt acattataca gcttcagcac tggcgctcgg actggcgtta atggggaatg
    33901 cacaggcagt gacgaccatt ccgttctggc attctatgga aggggaactg ggtaaagagg
    33961 tggattctct ggcccaacgt tttaacgccg aaaatccgga ttacaaaatt gtaccgacct
    34021 ataaaggcaa ctacgaacag aatttaagcg cggggattgc cgcatttcgt accggcaacg
    34081 ctccggctat tttgcaggtt tatgaagttg gcaccgccac catgatggca tcgaaagcca
    34141 ttaaaccggt atatgacgtg tttaaagagg cggggattca attcgatgag tcgcagtttg
    34201 tgccgacggt ttccggttac tactccgaca gcaaaacggg ccacttactc tcccagccat
    34261 tcaacagctc gacccccgtt ctctattaca acaaagacgc cttcaagaaa gcaggattag
    34321 acccggaaca accgccgaaa acctggcagg atctggcgga ctatgccgcg aaactgaaag
    34381 cctccggtat gaagtgcggc tacgccagcg gctggcaggg ctggatccaa ctggaaaact
    34441 ttagcgcctg gaacggtctg ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg
    34501 cggtgctgga gttcaacaag ccggagcagg tgaaacacat cgccatgctc gaggagatga
    34561 acaagaaggg cgacttcagc tacgtcggtc gtaaggatga atccaccgag aagttctata
    34621 acggtgattg cgcgatgacc accgcctctt ccggttctct cgctaatatt cgtgagtacg
    34681 ccaaatttaa ctatggcgta ggcatgatgc cttacgacgc cgatgcgaaa gatgcaccgc
    34741 aaaacgccat tatcggcggg gccagcctgt gggtgatgca gggcaaaaat aaagaaacgt
    34801 ataccggcgt ggcgaagttc ctcgacttcc tcgcgaagcc agaaaacgct gccgagtggc
    34861 atcagaaaac cggttatctg ccaatcacca aagcagcgta tgacctgacc cgtgagcagg
    34921 gcttttacga gaaaaaccca ggggcggata ccgcgacgcg tcagatgctg aataagccgc
    34981 cgttgccgtt caccaaaggg ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg
    35041 atgaagagct ggagagcgtg tggaccggta agaagacacc acagcaggca ctggataccg
    35101 ccgttgagcg tggaaatcag ttgctgcgcc gctttgagaa atcgacgaag tcttaatcag
    35161 tgtaatgtcg gatgcgtttc gcttatctga cctggcatcg cgtgtaggcc ggataagcga
    35221 agcgcatccg gcacagttca ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc
    35281 tcgcgctggc tgccttatct gctggtcgcg ccgcagctca tcatcaccgt tatctttttt
    35341 atctggcctg cgggcgaagc gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc
    35401 tccagccagt ttgtcggcct ggataacttc gtcgcgctgt ttcatgacag ctactatctc
    35461 gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg tttgctggtg
    35521 tcgctgttct tcgcggcgtt ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc
    35581 ttgatgctgc tgccgtatgc cgtggctccc gccgttgccg ccgtattgtg gatcttcctg
    35641 tttaaccccg gtcgtgggct gatcacccat tttctcgctg agttcggtta tgactggaac
    35701 catgcgcaga acagcggtca ggcaatgttt ctggtggtgt ttgcctcggt gtggaagcaa
    35761 atcagctaca actttctgtt cttctatgcc gcgctgcact ccattccccg ttcattgatt
    35821 gaagctgcag ccatcgacgg tgcaggccca attcgccgct tctttaagat tgcgctgccg
    35881 cttatcgctc cggtgagttt cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac
    35941 accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg
    36001 atttataaga tctaccgcga aggttttacc ggactggatc tggcttcgtc tgccgcacag
    36061 tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa
    36121 agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacgatattc agccatacca
    36181 tgctgatcct cgggatcgcg gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga
    36241 cgctggataa acaggccgtc tatgccgcgc cgatgacgct catccccggc acatatctgc
    36301 tggaaaatat ccacaacatc tgggttaacg gggtaggtac gaatagcgcg ccgttctggc
    36361 ggatgttgct taacagcttt gtgatggcgt tcagcattac gctcggcaaa attaccgtct
    36421 cgatgctctc ggcatttgcc attgtctggt ttcgttttcc gctacgtaac ctcttcttct
    36481 ggatgatttt tatcaccctg atgctgccgg ttgaagtacg tatcttcccg acggtggaag
    36541 tcatcgccaa cctgaagatg ctcgacagct acgccggttt aacgctgccg ctgatggcct
    36601 cggcgaccgc tactttcctg ttccgccagt tctttatgac gctgccggat gagctggtgg
    36661 aagccgcgcg gatcgacggc gcatcgccaa tgcgcttctt ttgcgacatc gtttttccgc
    36721 tctccaaaac taatctggcg gcgctgtttg tgatcacctt tatctacggc tggaatcagt
    36781 atttgtggcc gttgttgatt attaccgacg ttgatctcgg caccaccgtg gcagggatca
    36841 aagggatgat cgccactggc gaaggcacca cggaatggaa ctcagtgatg gcggcgatgt
    36901 tgttaaccct gatcccacca gtagtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg
    36961 gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt aaccaaaagc
    37021 tgggatggca aaacccaggt cattaaaccg ctgacccttg atgtggcaga tggcgaattt
    37081 atcgtgatgg tcgggccgtc tggctgcggg aaatcgacgc tgctgcgcat ggttgccggg
    37141 ctggagcggg tgaccgaagg cgatatctgg atcaacgacc agcgcgtgac cgaaatggag
    37201 ccgaaagatc gcgggattgc gatggtgttc cagaactacg cgctttatcc gcatatgagc
    37261 gtcgaagaaa acatggcgtg ggggctgaaa attcgcggca tgggcaagca gcaaatagcc
    37321 gagcgcgtta aagaagcggc gcgcattctg gagctggatg gcctgcttaa gcgccgcccg
    37381 cgcgagctat ccggcggtca gcgtcagcgt gtggcgatgg gtcgcgcgat tgtgcgcgat
    37441 ccggcggtgt tcctgtttga tgagccgctc tctaacctcg atgccaagct gcgcgtgcag
    37501 atgcgccttg agctgcaaca gctgcaccgt cgcctgaaaa cgacttcact ctacgttact
    37561 cacgatcagg ttgaagcgat gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt
    37621 gccgaacaga ttggcacacc agttgaagtc tacgaaaagc ccgccagcct gtttgtagcg
    37681 agttttatcg gcagtccggc gatgaacctg ctgacaggcc gcgtgaataa cgaaggcacg
    37741 catttcgaac tggacggcgg tattgagctg ccgctaaacg gtggctaccg tcagtatgcc
    37801 gggcgtaaaa tgactctcgg cattcgcccg gaacatattg cgctaagctc gcaggcagaa
    37861 ggcggcgtac cgctggtgat ggacacgctg gagatcctcg gcgcagataa cctggcgcac
    37921 ggacgctggg gcgagcagaa gctggtggtg cgactggcgc atcaggagcg cccgacggca
    37981 ggcagcacgc tgtggctgca tctggcggaa aatcagctgc atctttttga tggtgaaaca
    38041 ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgctcatcgt ggcggcggta
    38101 agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg ggcaaaatac ggtcataaga
    38161 tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat cttcctgctc catgacgaca
    38221 atctcgaacg caccagcaac ggctggggcg tcgcgggtga actgaactgg caggatttac
    38281 tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa aggtgagtcg ctgccgttgc
    38341 tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac
    38401 ccaccactgg cactggacca ttaacgggca aaatggtggc gctggcggca cgccaactgt
    38461 gggccggtat gacgccgccg ctgctgtcat cgtttgagat tgatgcttta gaagctgcac
    38521 agctggcggc accggaactg ccgcgtggtt tattgcttga tgagtggcgc gacgactggc
    38581 gcgaactgac cgcgcggctg ggctgcgtct ctattcatct caatcataag ttactcgata
    38641 aagtgcgagt gatgcagttg aaagacgccg gactgcggat tctggtttat accgtcaata
    38701 aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga
    38761 ttgacgtgat tggtccgaac tttacggccc aatagttttc aacggaatgt caggctgcgg
    38821 cgtgctgggc tgattcagca tgtcgccgtt tctcctctcc ggcagcatat gctgctgatt
    38881 caacgaacta tccggattac ggttgctgtt taacatcccg ccgttggtgt tgggcagcat
    38941 ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct gagaattatt
    39001 gtttatctgg ttttctaaat gctgctgttg caactgagtt tgcgttttca gttgctgatt
    39061 cagcatccct ttttgctgga tttgctgagt ctgcatctgg gtttgcatcc gctgctggct
    39121 gggtatctga taccccggct ggttagggtt gttcagagta ttaatgggct gtgcaaagcc
    39181 gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc ctcctctgaa
    39241 gatatccttt aagtttactc gcttcccgac aaaacgatga ttaattcaga gttatatacc
    39301 aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa ccgacgtttt
    39361 tacgccgggt ggccattgct gctctgctct caggaagttg ttttagcgcc gccgccgcgc
    39421 ctcctgcgcc gcccgtctcg tatggtgtgg aggaagatgt cttccacccg gtacgcgcga
    39481 aacagggaat ggtagcgtct gtggacgcca ctgccactca ggtgggggtg gatattctca
    39541 aggagggcgg gaatgccgtt gatgccgccg tggcggtggg ctacgcgctg gcggtaacgc
    39601 atccgcaggc agggaatctg ggcggtggtg gttttatgct gatccgctcg aaaaatggca
    39661 ataccaccgc tatcgatttc cgcgaaatgg cacccgctaa agcgacccgc gatatgttcc
    39721 ttgatgacca gggcaacccg gacagcaaaa aatcactcac ttcgcatctg gcttccggca
    39781 caccaggtac ggtcgctggt ttctcgctgg cgttggataa gtacggcact atgccgttga
    39841 acaaagtggt gcagcccgcg ttcaaactgg cacgcgatgg ttttatcgtt aacgatgctt
    39901 tggcagacga tctcaaaacc tacggtagcg aagtgttgcc gaatcacgaa aacagcaaag
    39961 ctatcttctg gaaagagggc gaaccgctga aaaagggcga cacgctggtg caggcgaacc
    40021 tggcaaagag cctggagatg attgctgaaa acggcccgga cgaattctat aaaggcacga
    40081 ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg cttgatcact aaagaagatt
    40141 tagcggccta taaagcggtc gaacgcactc cgataagcgg cgattatcgc gggtatcagg
    40201 tttactccat gccaccgcca tcctccggcg ggatccatat cgtacaaatc ctcaatattc
    40261 tggaaaactt cgatatgaag aaatacggct ttggcagcgc cgatgcgatg caaatcatgg
    40321 cagaagcgga gaaatacgcc tacgccgacc gctcggaata tcttggcgac ccggattttg
    40381 tcaaagtccc gtggcaggca ctgaccaata aagcctatgc caaatccatt gccgatcaaa
    40441 ttgatatcaa caaagcgaag ccgtccagtg agattcgccc tggcaagctt gcgccttatg
    40501 agagtaatca aactacccat tactctgtag tggataaaga cggtaacgcg gtggcggtga
    40561 cctatacgct gaacaccacc ttcggtacgg gtattgtcgc gggcgagagc ggtattctgc
    40621 ttaataacca gatggatgat ttctccgcca aaccgggcgt accgaacgtt tacgggctgg
    40681 tgggcggtga tgccaacgcc gtcgggccga acaaacgccc gctgtcgtcg atgtcgccga
    40741 ccattgtggt gaaagacggt aaaacctggc tggttaccgg tagcccaggc ggtagccgga
    40801 tcatcactac agtgctgcaa atggtggtga atagcatcga ttatggcatg aacgtcgccg
    40861 aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa
    40921 aagggtttag cccggatacg ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga
    40981 aagaggcgat gggcagtaca caaagcatta tggttgggcc ggacggtgag ttgtacggcg
    41041 catccgaccc gcgctcggtg gatgatttaa cggcggggta ctaaggttag cggccctctt
    41101 cgtgggaaga gggctatttt gtcagggcaa gccgaaggta gcctttttta tttcgtaatc
    41161 ctgtagatat tcttccagcg gctttgccgt tcccagagaa tgaatttcac cactgtcttt
    41221 atcaataata aaaggtgcgt taccagctaa gcgcgcggcc tcatctccag tttcgagaaa
    41281 ttctcgtgct tcgaaacaga aataccagcc ctggctaaag cgtccatgta gagtaatgac
    41341 gaccgggaga tctgcatcat caaggtaatg gttcgctttc gcgaatgcgt cgtgataagt
    41401 aatcataatt ataataaata ttattgttga gtgttatatt attattcctg tgtgatacat
    41461 tgagcaaaga cgcgttcatc ttcgtcaaag atattattaa atgctggttt tgaaaaaagt
    41521 tcatcggata agatatagtc acgaaataag tatctgctaa tattttatga tttctttttc
    41581 agaacgtcga acgggatttt cttatttaag atatcagaat cttatgattc gggtaaaatt
    41641 ctaaataaat caacatgtca ttaaaatcat catagccatt agctatttcg gctaaaatag
    41701 agactacatg tcttcggtcc atctcactta aggagtgtag ttccgttgta agtttttcca
    41761 tagcttgcac tgctaaattt cgaacaagga attttctgct ggtaatctct aaaaagatgg
    41821 catggtttac aatgattttt gtttcctttt gattattatg aacaactgtc catgatttcg
    41881 tttaagaatg aagagaaatc actaaacgaa ctgaatatat tttctgtgcc aatattatct
    41941 ctaatttcaa aaaagttact tttaatgtca taagtaaata ttgaaatgct atcatccccg
    42001 atcactaacc gttcttgtag atcagggttt atgaaatcat cattatttct ataaaattca
    42061 tttacggcaa aaaggttttt aactgaaggt tctggaatcg agaggctaaa taatcgtaat
    42121 ccattaactt cgaatccatc cattgctctt aaaaaagtaa gataatcagg ttgattctta
    42181 aaaagaatac taacttcatt acataacttc tccaggcaat tagttgaatt agaattaaat
    42241 tctatttgga ttatactttc aataaatgat tccttgagcg gtgcttcaaa tggatagtct
    42301 aaatcattca tcatttttat taatttattt atttgggttt tagtcatcat tttgtgtggt
    42361 ccataatatt tttcatggga ggataaattg agccagatag aatggcccag ggagtgattt
    42421 tgctttcacc tactaatatt cccttagcta ttcgtgattg catgttagta aaaactttat
    42481 gatatggttc gttttcgatt aatactaaat tttcgaaatc attagtacca ccatcatcta
    42541 gagatagttt atggtgaaca ttataattta gaggaacatt gccctttctc attcttaaaa
    42601 ggtcttcggc gttgaatctt ccagatgctt cggaggtttt tgcaagtttg ataagaaaat
    42661 tttttcttac agttttatta aactcctttc gtaagttagc taagtctttt gaaggtctct
    42721 taatgtatat tatttctatt atgtctatac catcaagaag tttttgttga cctctaagtt
    42781 tgcctaagta actatttatt tctgtatatg tatcaacatt agaacgcttt gttaaaagaa
    42841 tagatgctgt tgctatcgct atgcccgctg ttttttcatt tagtatttga tcatagcctt
    42901 ttattgcatc tccacccagt ttttccgcag tatctctgaa tccttcaatg ttcccattgt
    42961 atacgccacc agcagcaagt agcctgccaa tagctttgc
//
LOCUS       DAFAVF010000020        42873 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFAVF010000020 DAFAVF010000000
VERSION     DAFAVF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.020"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(180..855)
                     /locus_tag="LGK42_001105"
                     /pseudo
     CDS             complement(180..855)
                     /locus_tag="LGK42_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1000..1608)
                     /locus_tag="LGK42_001106"
     CDS             complement(1000..1608)
                     /locus_tag="LGK42_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBK2657380.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1792..2109)
                     /gene="metJ"
                     /locus_tag="LGK42_001107"
     CDS             complement(1792..2109)
                     /gene="metJ"
                     /locus_tag="LGK42_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBK2657381.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2386..3546
                     /gene="metB"
                     /locus_tag="LGK42_001108"
     CDS             2386..3546
                     /gene="metB"
                     /locus_tag="LGK42_001108"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK2657382.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3549..5981
                     /gene="metL"
                     /locus_tag="LGK42_001109"
     CDS             3549..5981
                     /gene="metL"
                     /locus_tag="LGK42_001109"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBK2657383.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            6330..7220
                     /gene="metF"
                     /locus_tag="LGK42_001110"
     CDS             6330..7220
                     /gene="metF"
                     /locus_tag="LGK42_001110"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBK2657384.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7549..9729
                     /gene="katG"
                     /locus_tag="LGK42_001111"
     CDS             7549..9729
                     /gene="katG"
                     /locus_tag="LGK42_001111"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBK2657385.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9823..10728
                     /gene="yijE"
                     /locus_tag="LGK42_001112"
     CDS             9823..10728
                     /gene="yijE"
                     /locus_tag="LGK42_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBK2657386.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10755..11372)
                     /locus_tag="LGK42_001113"
     CDS             complement(10755..11372)
                     /locus_tag="LGK42_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBK2657387.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11647..12750)
                     /gene="gldA"
                     /locus_tag="LGK42_001114"
     CDS             complement(11647..12750)
                     /gene="gldA"
                     /locus_tag="LGK42_001114"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBK2657388.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12761..13423)
                     /gene="fsa"
                     /locus_tag="LGK42_001115"
     CDS             complement(12761..13423)
                     /gene="fsa"
                     /locus_tag="LGK42_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK2657389.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13435..15936)
                     /gene="ptsP"
                     /locus_tag="LGK42_001116"
     CDS             complement(13435..15936)
                     /gene="ptsP"
                     /locus_tag="LGK42_001116"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2657390.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16245..17324
                     /locus_tag="LGK42_001117"
     CDS             16245..17324
                     /locus_tag="LGK42_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBK2657391.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17339..17659
                     /locus_tag="LGK42_001118"
     CDS             17339..17659
                     /locus_tag="LGK42_001118"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBK2657392.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17710..20007
                     /locus_tag="LGK42_001119"
     CDS             17710..20007
                     /locus_tag="LGK42_001119"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK2657393.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAELTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19973..20851
                     /locus_tag="LGK42_001120"
     CDS             19973..20851
                     /locus_tag="LGK42_001120"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBK2657394.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20853..21194
                     /locus_tag="LGK42_001121"
     CDS             20853..21194
                     /locus_tag="LGK42_001121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBK2657395.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21181..22032)
                     /locus_tag="LGK42_001122"
     CDS             complement(21181..22032)
                     /locus_tag="LGK42_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2657396.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22182..23915)
                     /locus_tag="LGK42_001123"
     CDS             complement(22182..23915)
                     /locus_tag="LGK42_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBK2657397.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24098..26749)
                     /gene="ppc"
                     /locus_tag="LGK42_001124"
     CDS             complement(24098..26749)
                     /gene="ppc"
                     /locus_tag="LGK42_001124"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBK2657398.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27101..28252)
                     /gene="argE"
                     /locus_tag="LGK42_001125"
     CDS             complement(27101..28252)
                     /gene="argE"
                     /locus_tag="LGK42_001125"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBK2657399.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28406..29410
                     /gene="argC"
                     /locus_tag="LGK42_001126"
     CDS             28406..29410
                     /gene="argC"
                     /locus_tag="LGK42_001126"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBK2657400.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29418..30194
                     /gene="argB"
                     /locus_tag="LGK42_001127"
     CDS             29418..30194
                     /gene="argB"
                     /locus_tag="LGK42_001127"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBK2657401.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30255..31628
                     /gene="argH"
                     /locus_tag="LGK42_001128"
     CDS             30255..31628
                     /gene="argH"
                     /locus_tag="LGK42_001128"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBK2657402.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32126..33340
                     /locus_tag="LGK42_001129"
     CDS             32126..33340
                     /locus_tag="LGK42_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBK2657403.1"
                     /translation="MKIIAADVFVTCPGRNFVTLQITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33407..34690
                     /locus_tag="LGK42_001130"
     CDS             33407..34690
                     /locus_tag="LGK42_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657404.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34942..35859
                     /gene="oxyR"
                     /locus_tag="LGK42_001131"
     CDS             34942..35859
                     /gene="oxyR"
                     /locus_tag="LGK42_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBK2657405.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35842..37242)
                     /gene="sthA"
                     /locus_tag="LGK42_001132"
     CDS             complement(35842..37242)
                     /gene="sthA"
                     /locus_tag="LGK42_001132"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBK2657406.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37573..38223
                     /gene="fabR"
                     /locus_tag="LGK42_001133"
     CDS             37573..38223
                     /gene="fabR"
                     /locus_tag="LGK42_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBK2657407.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38223..38582
                     /locus_tag="LGK42_001134"
     CDS             38223..38582
                     /locus_tag="LGK42_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBK2657408.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38622..39722)
                     /gene="trmA"
                     /locus_tag="LGK42_001135"
     CDS             complement(38622..39722)
                     /gene="trmA"
                     /locus_tag="LGK42_001135"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBK2657409.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39836..40026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40091..41935
                     /gene="btuB"
                     /locus_tag="LGK42_001136"
     CDS             40091..41935
                     /gene="btuB"
                     /locus_tag="LGK42_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBK2657410.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41880..42737
                     /gene="murI"
                     /locus_tag="LGK42_001137"
     CDS             41880..42737
                     /gene="murI"
                     /locus_tag="LGK42_001137"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK2657411.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9755 a        10672 c        11806 g        10640 t
ORIGIN      
        1 gacataatgg ggggcggggg aatgtcatat gcctatgaat atgatgtaaa atataatata
       61 tgtcatttga gaaaaaagag cagaatgagg taaaaatctt tggctgtgta ttcagtctgg
      121 taatcatcca aggaaagctt atcagaaggg tgatgactcc aattagtaga acatatattt
      181 tcgataaaag caccgatgac tttttcatga agttctacag aacgcgagaa acagatggtt
      241 ctgcgggcca gacgtttgat tcgggttctc agggtcagat tgttacgctc aatgtgctga
      301 gtaaaaatct taccagtcag atgcttctcc ttcggtaact ctctagcgta actaccccat
      361 tcgtcagtgg tcaacatgcc gatggtaaaa gggtaagcag cgcccgcaat tcacgacagg
      421 tttcgtcagt ccgggggcca aaagtgtagg ccagaacacc accagttttc gtgttgtaca
      481 cgtaccagag ccagtgctgt cgcgctttac tgccgacaaa gctccattgc tgactaagtt
      541 tgcagatgag tgccacatca gcatgagcga ccggggatga cgttattctt cgtggcgaga
      601 gttttttaaa gttcggatga ctgtattaat gccgattttc agcgtacggg cagtatcgcg
      661 aaccccggca ccgttgaaag tcatttcagc gatttggtct ttaactcccg gtttacgggg
      721 ctcgtaacag taactgagct gaaatacccg gtgacagtca cggccgcgga atcgatcatg
      781 tctttttggg ttctgcccgt gacggtaaac ctgaacggac tggcaacggg ggcaatgaag
      841 gttaactgtg accatgaaac aaccttaaaa gcgagtatta tatccttaat tcaactaatt
      901 ggaggcatca ccgtttaccg gagcgacagc ttgcggaaag gtcgtgcaac tacaaagcaa
      961 caaacagact acggcgggaa ttattttttt catcatccat taccacgaaa acagcggcca
     1021 ctctttggca accgtggtga gttgtggcgc gtcgaaaacg gcttgtggtg aaactaccgt
     1081 ttcctcaagc ggaatatttt taccgtagcg ttctttgagc ttcgcctgga ccagcgggtt
     1141 gctgagatca aactctttca acttctgctg ttcgcccacg cgcattccca gcgcgttctg
     1201 gtaaaccttc cacaccactt ccgaacagta ctggcgatcg tcgctccatg agaagctgaa
     1261 atcatacggt ttaccgagat aacgttttgc cgtttgcgcc agtttttgct gttgttcaac
     1321 actcagtccg ccttcaacgc ggcgaacgac gtatttgccc ttttcaccat gggcaatcca
     1381 ctgcttgagc ggggtgtatt tcaccgggcc gaccgcttca aaaacgtagg gctttttgtt
     1441 acgcatcacc agcataccgg tgtggctgta atcggaatgg gttgccagtt ggatcgcttt
     1501 actttgcgat gagcgagaga tttgaaagat gatgtcgcca gtttgcggct gccaggcaaa
     1561 ggcaggtaca gaaaccagca ggctgaggat cagcagcctg tttttcatag ttaaacgtcc
     1621 atgtataaaa agcggtgggt cgcagacaac gtgctcgttg tttatgccgg atgcggcgtg
     1681 aacgccttat ccggcctaca agttcgtgca aattcaataa attgcaatat gacgtaggcc
     1741 tgataagcgt agcgcatcag gcggttccac tccgcgccgc tcttttccgc tttagtattc
     1801 ccacgtctcc gggttaatcc ccatctcacg catgatctct tttgccgctt ccgggatttc
     1861 gtcgctgcgc tctttacgca gatcggcatc atccggcaaa ggttgcccgg taaaggcatg
     1921 cagaaacgct tcgcacagca gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg
     1981 acgcgtgcgt tcatcggtga ggatttttaa caccttaaga ggaatggaaa ccgtaatctt
     2041 tttgacttgt tcactcttct tgccgtgctc agcgtatggg ctgatatatt cgccgctcca
     2101 ttcagccatg agatacttaa tcctcttcgt caataaattg agaccagacc acacagttaa
     2161 tgtgggcact ggccgtaaac ccgcatagtt taccgtacag gcgctaccgt tacatcgggt
     2221 aatccacctg ttggcgtgat aatgcatata attttaacgg ctatttggga tttgctcaat
     2281 ctatacgcaa agaagtttag atgtccagat gtattgacgt ccattaccac aatgtttact
     2341 ctggtgcctg acatttcacc aataaagccc agggaacatc atcacatgac gcgtaaacag
     2401 gccaccatcg cagtgcgtag cgggttaaat gacgacgaac agtatggttg cgttgtccca
     2461 cccatccatc tttccagtac ctataacttt accggattta atgaaccgcg cgcgcatgat
     2521 tactcgcgtc gtggcaaccc aacgcgcgat gtggttcagc gagcactggc agaactggaa
     2581 ggcggtgctg gtgcagtact tactaatacc ggcatgtccg cgattcacct ggtaacgacc
     2641 gtctttttga aacctggcga tctgctggta gcgccgcacg actgctacgg cggtagctat
     2701 cgcctgttcg acagcctggc gaaacgtggt tgctatcgcg tgttgtttgt tgatcaaggc
     2761 gatgaacagg cattacgggc agcgctggca gaaaaaccca aactggtact ggtagaaagc
     2821 ccaagtaatc cattgttacg cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa
     2881 gtcggggcgg tgagcgtggt ggataacacc ttcttaagcc cggcattaca aaatccgctg
     2941 gcattaggtg ccgatctggt gttgcattca tgcacgaaat atctgaacgg tcactcagac
     3001 gtggtggccg gcgtggtgat tgctaaagac ccggacgttg tcactgaact tgcctggtgg
     3061 gcaaacaata ttggcgtgac gggcggcgcg tttgacagct atctgctgct acgcgggttg
     3121 cgaacgctgg tgccgcgcat ggagttggcc cagcgtaacg cgcaggcgat tgtgaaatat
     3181 ctgcaaaccc agccgttggt gaaaaaactg tatcacccgt cgctgccgga aaatcagggg
     3241 catgaaattg ccgcgcgtca gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat
     3301 ggcgatgagc agacgctgcg tcgtttcctg ggcgggctgt cgttgtttac gctggcggaa
     3361 tcattagggg gagtggaaag tttaatctct cacgccgcaa ccatgacaca tgcaggcatg
     3421 gcaccagaag cgcgtgctgc cgccgggatt tccgagacgc tgctgcgtat ctccaccggt
     3481 attgaagatg gcgaagattt aattgccgac ctggaaaatg gcttccgggc tgcaaacaag
     3541 gggtaaaaat gagtgtgatt gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg
     3601 gtggcagtag tctggctgat gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt
     3661 actctcagcc tgacgatatg atggtggttt ccgccgccgg tagcaccact aaccagttga
     3721 ttaactggtt gaaactaagc cagaccgatc gtctctctgc gcatcaggtt caacaaacgc
     3781 tgcgtcgcta tcagtgcgat ctgattagcg gtctgctacc cgctgaagaa gccgatagcc
     3841 tcattagcgc ttttgtcagc gaccttgagc gcctggcggc gctgctcgac agcggtatta
     3901 acgacgcagt gtatgcggaa gtggtggggc acggtgaagt gtggtcggca cgcctgatgt
     3961 ctgcggtact taatcaacaa gggctgccag cggcctggct tgatgcccgc gagtttttac
     4021 gcgctgaacg cgccgcacaa ccgcaggttg atgaagggct ttcttacccg ttgctgcaac
     4081 agctgctggt gcaacatccg ggcaaacgtc tggtggtgac cggatttatc agccgcaaca
     4141 acgccggtga aacggtgctg ctggggcgta acggttccga ctattccgcg acacaaatcg
     4201 gtgcgctggc gggtgtttct cgcgtaacca tctggagcga cgtcgccggg gtatacagtg
     4261 ccgacccgcg taaagtgaaa gatgcctgcc tgctgccgtt gctgcgtctg gatgaggcca
     4321 gcgaactggc gcgcctggcg gctcccgttc ttcacgcccg tactttacag ccggtttctg
     4381 gcagcgaaat cgacctgcaa ctgcgctgta gctacacgcc ggatcaaggt tccacgcgca
     4441 ttgaacgcgt gctggcctcc ggtactggtg cgcgtattgt caccagccac gatgatgtct
     4501 gtttgattga gtttcaggtg cccgccagtc aggatttcaa actggcgcat aaagagatcg
     4561 accaaatcct gaaacgcgcg caggtacgcc cgctggcggt tggcgtacat aacgatcgcc
     4621 agttgctgca attttgctac acctcagaag tggccgacag tgcgctgaaa atcctcgacg
     4681 aagcgggatt acctggcgaa ctgcgcctgc gtcaggggct ggcgctggtg gcgatggtcg
     4741 gtgcaggcgt cacccgtaac ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc
     4801 agccggtcga atttacctgg cagtccgatg acggcatcag cctggtggca gtactgcgca
     4861 ccggcccgac cgaaagcctg attcaggggc tgcatcagtc cgtcttccgc gcagaaaaac
     4921 gcatcggcct ggtattgttc ggtaagggca atatcggttc ccgttggctg gaactgttcg
     4981 cccgtgagca gagcacgctt tcggcacgta ccggctttga gtttgtgctg gcaggtgtgg
     5041 tggacagccg ccgcagcctg ttgagctatg acgggctgga cgccagccgc gcgttagcct
     5101 tcttcaacga tgaagcggtt gagcaggatg aagagtcgtt gttcctgtgg atgcgcgccc
     5161 atccgtatga tgatttagtg gtgctggacg ttaccgccag ccagcagctt gctgatcagt
     5221 atcttgattt cgccagccac ggtttccacg ttatcagcgc caacaaactg gcgggagcca
     5281 gcgacagcaa taaatatcgc cagatccacg acgccttcga aaaaaccggg cgtcactggc
     5341 tgtacaatgc caccgtcggt gcgggcttgc cgatcaacca caccgtgcgc gatctgatcg
     5401 acagcggcga tactattttg tcgatcagcg ggatcttctc cggcacgctc tcctggctat
     5461 tcctgcaatt cgacggtagc gtgccgttta ccgagctggt ggatcaggcg tggcagcagg
     5521 gcttaaccga gcctgatccg cgtgacgacc tctccggcaa agacgtaatg cgcaagctgg
     5581 taattctggc gcgtgaagcg ggttacaaca ttgaaccgga ccaggtacgt gtggaatcgc
     5641 tggtgcctgc tcattgcgaa ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac
     5701 ttaacgagca gatggtgcaa cggctggaag cggcccgcga aatggggctg gtgctgcgct
     5761 acgtggcgcg tttcgatgcc aacggcaaag cgcgtgtggg cgtggaggcg gtgcgtgaag
     5821 accatccgct ggcatcactg ctgccgtgcg ataacgtctt tgccatcgaa agccgctggt
     5881 atcgcgacaa cccgctggtg atccgtgggc ctggcgcggg gcgcgacgtc accgccgggg
     5941 cgattcagtc ggatatcaac cggctggcac agttgttgta atttcagaaa tttaataatg
     6001 cccggtactc atgttttcgg gtttatggtt tctaatgaaa tatattgaat tatcatagga
     6061 ttaggccgga ttaagcgttt acgacgaatc cggcaagaag caataagtac atggttagtt
     6121 tatatttgca gtccggtttg ctttgcatac cggattttct ttttcttacc atcctgaagt
     6181 tttttcatct tccctgattt ttcctcacca tcattggtca tttttcggtt gacgcccttc
     6241 ggcttttcct tcatctttac atctggacgt ctaaacggat agatgtgcac aacacaacat
     6301 ataactacaa gcgattgatg aggtaaggta tgagcttttt tcacgccagc cagcgggatg
     6361 ccctgaatca gagcctggca gaagtccagg ggcaaattaa cgtttcgttc gagtttttcc
     6421 cgccgcgtac cagtgaaatg gagcagaccc tgtggaactc catcgatcgc cttagcagcc
     6481 tgaaaccgaa gtttgtatcg gtgacctatg gcgcgaactc cggcgagcgc gaccgtacgc
     6541 acagcattat taaaggcatt aaagatcgca ctggtctgga agcggcaccg catcttactt
     6601 gcattgatgc gacgcccgac gagctgcgca ccattgcacg cgactactgg aataacggta
     6661 ttcgtcatat cgtggcgctg cgtggcgatc tgccgccggg aagtggtaag ccggaaatgt
     6721 atgcttctga cctggtgacg ctgttaaaag aagtggcaga tttcgatatc tccgtggcgg
     6781 cgtatccgga ggttcacccg gaagcaaaaa gcgctcaggc ggatttgctc aacctgaaac
     6841 gcaaagtcga tgccggagcc aaccgcgcga ttactcagtt cttctttgat gtcgaaagct
     6901 acctgcgttt tcgtgaccgc tgcgtatcgg cgggtattga tgtggaaatt attccgggaa
     6961 ttttgccggt ctcgaacttt aaacaagcga agaaatttgc cgatatgacc aacgtgcgta
     7021 ttccggcgtg gatggcacaa atgtttgacg ggctggacga cgatgccgaa acccgcaaac
     7081 tggtgggagc gaatattgcc atggatatgg tgaagatttt aagccgtgaa ggagtgaaag
     7141 atttccactt ctatacgctt aaccgcgctg aaatgagtta cgcgatttgc catacgctgg
     7201 gtgttcgacc tggtttataa atagtgtggc ttttgtgaaa atcacacagt gatcacaaat
     7261 tttaaacaga gcacaaaatg ctgcctcgaa atgagggcgg gaaaataagg ttatcagcct
     7321 tgttttctcc ctcattactt gaaggatatg aacctaaaac ccttttttat aaagcatttg
     7381 tccgaattcg gacataatca aaaaagctta attacgatca atttgatcta catctcttta
     7441 accaacaata tgtaagatct caactatcgc atccgtggat taattcaatt ataacttctc
     7501 tctaacgctg tgtatcgtaa cggtaacact gtagagggga gcacattgat gagcacgtca
     7561 gacgatattc ataacaccac agccactggc aaatgcccgt tccatcaggg cggtcacgac
     7621 cagagcgcgg gggcgggcac aaccactcgc gactggtggc caaatcaact ccgtgttgac
     7681 ctgttaaacc aacattctaa tcgttctaac ccactgggtg aggactttga ctaccgcaaa
     7741 gaattcagca aattagatta ctacggcctg aaaaaagatc tgaaagccct gctgacagaa
     7801 tctcagccgt ggtggcctgc cgactggggc agttacgccg gtctgtttat tcgtatggcc
     7861 tggcacggcg cgggtactta ccgttcaatc gatggacgcg gcggcgcggg tcgtggtcag
     7921 caacgttttg caccgctgaa ctcttggccg gataacgtaa gcctcgataa agcgcgtcgc
     7981 ctgttgtggc caatcaaaca gaaatatggt cagaaaatct cctgggccga cctgtttatc
     8041 ctcgcaggta acgtggcgct ggaaaactcc ggcttccgta ccttcggttt tggtgccggt
     8101 cgtgaagacg tctgggaacc ggatctggat gttaactggg gtgatgaaaa agcctggctg
     8161 actcaccgtc atccggaagc gctggcgaaa gcaccgctgg gtgcaaccga gatgggcctg
     8221 atttacgtta acccggaagg cccggaccac agcggcgaac cgctttctgc ggcagcagct
     8281 atccgcgcga ccttcggcaa catgggcatg aacgacgaag aaaccgtggc gctgattgcg
     8341 ggtggtcata cgctgggtaa aacccacggt gccggtccga catcaaatgt aggtcctgat
     8401 ccagaagctg caccgattga agaacaaggt ttaggctggg cgagcactta cggcagcggc
     8461 gttggcgcag atgccattac ctctggtctg gaagttgtct ggacccagac gccgacccag
     8521 tggagcaact atttcttcga gaacctgttc aaatatgagt gggtacagac ccgcagtccg
     8581 gctggcgcaa tccagttcga agcggtagac gcaccggaaa ttatcccgga tccgttcgat
     8641 ccgtcgaaga aacgtaaacc gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct
     8701 gagttcgaga agatctctcg tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt
     8761 gcccgtgcct ggttcaaact gacgcacagg gatatggggc cgaaatctcg ttacatcgga
     8821 ccggaagtac cgaaagaaga tctgatctgg caagatccgc tgccgcagcc gatctacaac
     8881 ccgaccgagc aggacattat cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt
     8941 agtgagctgg tatcggtggc ctgggcatct gcttctacct tccgtggtgg cgacaaacgc
     9001 ggtggtgcca acggtgcgcg tctggcatta atgccgcagc gcgactggga tgtgaacgcc
     9061 gcagccgttc gtgctctgcc tgttctggag aaaatccaga aagagtctgg taaagcctcg
     9121 ctggcggata tcatcgtgct ggctggtgtg gttggtgttg agaaagccgc aagcgccgca
     9181 ggtttgagca ttcatgtacc gtttgcgccg ggtcgcgttg atgcgcgtca ggatcagact
     9241 gacattgaga tgtttgagct gctggagcca attgctgacg gtttccgtaa ctatcgcgct
     9301 cgtctggacg tttccaccac cgagtcactg ctgatcgaca aagcacagca actgacgctg
     9361 accgcgccgg aaatgactgc gctggtgggc gggatgcgtg tactgggtgc caacttcgat
     9421 ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat tgagcaatga cttcttcgtg
     9481 aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg aatcgaaaga gctgtttgaa
     9541 ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca gccgtgcgga tctggtattt
     9601 ggttctaact ctgtcctgcg tgcggtggct gaagtttacg ccagcagcga tgctcacgag
     9661 aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac
     9721 ctgctgtaat ctgaccttct tcagcgactg cctttcaggc agtcgctgaa gtttctttac
     9781 cggcgtatag tgtccacagg aaaactacac actggatctc tcatgtctgt cgcaggaaag
     9841 agcaacccac tggcaatcag tggcctggtt gtgctcacac ttatctggag ttatagctgg
     9901 attttcatga agcaagtcac cagctacatc ggtgccttcg actttaccgc cttacgctgc
     9961 attttcggcg ctctcgtttt attcatcgtc cttttattac gtggtcgcgg aatgcgcccg
    10021 acaccgttta aatacacctt agccattgcc ctgttacaaa cctgcgggat ggttggcctg
    10081 gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg cgatcctaag ctataccatg
    10141 ccgttctggg tggtgatttt cgccgcgttg tttctcggtg aacgcctgcg acgtgggcaa
    10201 tatttcgcga tactgattgc cgctttcggc ttatttttgg tgttgcagcc gtggcaactc
    10261 gatttctctt cgatgaaaag tgccatgctg gcaatcctct ccggcgtcag ttggggggcg
    10321 agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc gcgtggattt attgtcgtta
    10381 acatcctggc agatgctcta tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg
    10441 caacgtgaaa ttgactggca gcccaccgtg ttctgggcgc tggcctacag tgcgattctg
    10501 gcgacggcgc tggcgtggag cttatggttg tttgtattga aaaacttgcc tgccagtatt
    10561 gccagcttaa gcacgctggc cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc
    10621 ggtgagaatc cgggggccgt tgaaggtagc ggtattgtgc tgattgtgct ggcactggcg
    10681 ctggtgagcc gtaagaaaaa agaagctgtc agtgtaaaaa gcatctgaat tttttcttca
    10741 tgtggggcga tcgcttattt aacaaaataa cgataatgcc ccaccatccg ccagctaaac
    10801 agcacatctt cttcctgcgc gcctgcgcca atgttatgta tcaccagcgg cgtaccgtcg
    10861 cgggcgaagc catctgacac caccccaata tgtgccagtc cgttatccag tcgccaggag
    10921 acaatatcgc ccgcttgata gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg
    10981 cgggtaaacc aggtttccag attaggcacc cgacggtgat cgatgttgct gtccgggcgc
    11041 tttaactgcc atttttgcgg gtactcggca aaattcttcg ccatatcttc gtgcaccagt
    11101 ttctgcaaat cgaccttctg gctgcgcaat gcacggatca ccacatcgga acataccccg
    11161 cgttcttgcg ggacatcacc gccaggatag gtaagctgca tatacgccgg atcgtaaaat
    11221 agcgtgctgc cgatttgctg tctggcaccg tctgcgatgg caaggttggt attggcctgg
    11281 atttgtacca cggttggtgg aacggcggga gattttaagg agtggctggt aaatgccgtt
    11341 agcaggctga gcagcgccag tgaagctttc atctggttcg tccgtgaaaa taagagtcac
    11401 agattcgacc ttccgggcta acgattgttc tgtggagaaa atgtccagaa gcggataaaa
    11461 aactcctgaa caacgtggga gtttcagatt actggcacag agtgccggat gcggagtgaa
    11521 cgccttatcc ggcctacaaa agcacgcata ttcaatatat tgcagagatt tcgtaggctt
    11581 gataaacgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc gggagtttgg
    11641 agtaggttat tcccactctt gcaggaaacg ctgaccgtac tggtcggcta ccagcagagc
    11701 ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tgaatggttt caccttctgc
    11761 acatgccgct tctgccacaa ttcgcatttt cgccgggaca tcttctttaa tatccagttg
    11821 agcgagagtt attggcaaac ctaccgcatg gctaagtgca gctacggttt cgatttcctc
    11881 aaccggtgcg ttttccagca ccagctgcgt cagcgtacca aatgccactt tttcaccgtg
    11941 ataatagtga tgcgcgtccg ggatagcggt caggccgtta tgcaccgcgt gagccgccgc
    12001 cagcccgccg ctttcaaaac caacgccgct caaataggtg ttcgcttcaa tcacgcgctc
    12061 cagcgccggg gttactacat gctgttcggc ggcaagcatc gctttttcgc cttcttccag
    12121 cagggtgttg tagcacagtt cagccagtgc cagcgccgcc tgggtgcact tgccgcccgc
    12181 catggtggtc gcgccgctac gagagcaagc acgcgcttca aaccaggttg ccagcgcatc
    12241 gccgataccc gctgctaaca gacgtgcagg tgcgcccgcg acgattttgg tgtcgacaat
    12301 gaccatattc gggttatttg gcaacagcag atagcggtca aactcaccct catcggtgta
    12361 gataacagac aatgcgctgc acggtgcatc ggtagaggcg atagtcggtg cgatcgctac
    12421 cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg agggtttttc cgccaccgat
    12481 accgagaatt gcgccacact gcgcagtctc cgcgatgcca cgcagacggt cgatctcatt
    12541 ttgcgaacat tcaccgccaa acggcgcaat ttctactacc agtccagcat ctttaaagct
    12601 tttctcgaca gtggattgag caaaacctaa aacaaatttg tcacccacca ctaaccagcg
    12661 ttctgccagc ggcttcaggt attcgcccag acgattaatc acatcagcgc cctggatgta
    12721 tttacccggt gattgaataa tgcggtccat aattgctcct ttagagatga gtagtgccaa
    12781 atgcggcatt ccagtcgtgt tcgaacttct ctatagctga ctctaccgca ggggtgttga
    12841 gcatttgttg cgctacatct aagggcaggg tgatggattc acatcctgcc agtaaacagt
    12901 ccagcgcctg acgcggcgtt ttaaagctgg ccgccagcac catgctttct ggtgcgtgca
    12961 tttctaacag cgcttgcagc tcctgaaccg tacgaatgcc gtccccgccc tgggcatcta
    13021 cgcggttaac atacggagca acgtattttg cccctgccag cgcggccagt aacccttgtg
    13081 cagcactgta cactgcggtg ccaagcgtag taataccctc ttttttcagc atcttaatgg
    13141 cggccagacc ttcggaagtc accgggattt tcaccacaat gcccggaatg gcgtcgcgca
    13201 ggcgcttcgc ttcttccacc ataccctgcg cgtcacgact catagtctga gcaaacagaa
    13261 tgccctcatc accaatcgct ttttgcagac gcggcagaac ttcccatatg gactccttgc
    13321 tggcagcgat aatgctcggg ttagttgtca ccccggcaat ggggaatatg cgtgccagac
    13381 gttcgacttc tgcgacgtta gcggtgtcca gatacagttc catgatgttt ccttttacag
    13441 ttccagttca tgttgcagca ggctggcgat agcgtcggca gaggcggcgt taaccagcgc
    13501 gttacggaat tcttcgtgca tgatgcggcg agcgaggcgc gagaaaatgc gcatgtgctg
    13561 atcgcccgca gcgtgtttgt tcagggttaa cataatgatg aattgcgctt catcatcgcc
    13621 ccagcgcacc ggcgcttgca gacgcgccac gctgatggtg gattgctcaa tgtgttctga
    13681 tttgctgtgt ggaatggcaa aactaaagcc cagaccggta gagaaaacgg cctcgcgcgc
    13741 ccataagtcg gcttccagtt tacgcggata gcggcagcgg cccgccagca gcaggttatc
    13801 ggtcatgcct ttgagcactt cttctttgct gcgccagtca ctttccagcg tgatgcactc
    13861 ggcggtgacc agcggtgcgt cctgttgggt catgcggaat tgcgccagca ggtgttccac
    13921 ttccagcgaa gtacggcagg ccattgcctg gttgagcaac ttgcggcact cacggctatc
    13981 aagttgcgcc atccgcgctt tcgccgccgg aattgatggt gcgctcatgc tgagttcatc
    14041 cagccctaag ccgaccagca acggcagcac ggaacctttc gctcccagct caccgcacag
    14101 accaatccat ttgccctggc gatgcaccgc ctgcacggcg taatcgagcg cccgcaagaa
    14161 tgccggattc aggctgttgt agtgacgagt aaccttagcg ttatcgcgat ccactgccag
    14221 cagatattgc gtcaggtcat tactaccaat actaaagaaa tcaatctctt cgcagcattg
    14281 atcgatgatg aacatcaccg acggtacttc cagcataatg ccaagctgga tcttctcatc
    14341 aaacggaatg tgttcgttac gcagttgctg tttggcttcc gccagttttt ctttcaccca
    14401 taagatctct tccattgagg agatcatcgg gatcatgatt ttcaggctgc cgtgagcgga
    14461 ggcgcgcagg atcgaccgta gttgtgtggt gaacagcgag gcgtattctt cataaatacg
    14521 cacagcgcga taaccgagga acgggttagc ttctgccgga atgttcagat aatcaacggg
    14581 tttgtcgccg ccaatgtcca tagtgcgcac aataatgctg cgtccgttgg cagactccag
    14641 cgcctgacaa aaaatgttgt acagctcgct ttcgcccggt gcactggtgc gatccatata
    14701 gagcatttca gtgcggaaca aaccaacgcc ttccgcacca ttgccgaatg cggcctgcgc
    14761 ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg ataccgtcag cggtacgggc
    14821 ttgttgggtc agccagacac gctgttgctc acgcagggcg tcctgtacgc gggcttcttg
    14881 ctgataataa cgagctacgg cttctcctgg ctcaaccaca atcgccccag cgttgccgtc
    14941 gatataaatc gtttgatgct gccacggagt aagggcatca atatctacac caaccagtgt
    15001 cggaatgttg aacgaacggg caagaattac ggtatgtgag gtggtgccgc cacttttcag
    15061 taacagacct ttgaggtgat ttttatccag ttcgaggaac tggctgggag tcagttcatc
    15121 agccatacaa atggcgggct gcgtcagttt gcccggtgcc gggaagcgtt gctcaccgta
    15181 gatttgctgg agtaactgga agcagacgtc gcgtacgtcc agggcacgtt cttgcaggta
    15241 gctgctgctg gaacgggcga actcttcgca aaagtgattc gcactggtga caatcgcttc
    15301 ggcgcagctt aatccggcgc tgacgcctgc cagtaaatgt tcgcgcaggg aagtatcgcc
    15361 agccagggat cggtgcgctt ccagaatcgc gctggtggca ccgtcgctat ccagcagacg
    15421 aaactcaatg tttttcagta ccagcgtcag gccgttttcc agtgcggatt gctcggcgtc
    15481 aacgcctttg gctgcgggaa gattacccag cgcattgaga tctaaagaag agatcggcgt
    15541 cagaatgccg cctgcactac cgctgcacac ggtgcgggcg cggataattt gcggattcag
    15601 attggtcagt gaaaccggca gtggttccag ttcgtcagat ttaacttccg ccagcggcgc
    15661 atcgcagtgg gggaattcat cgcgcagcca ttggcttaaa cgctggtgcg cttcctgttc
    15721 gtcggccccg gaaatcagta actggcagtt atcgcccgcc agcgtatcgg tgccaatcag
    15781 cgcaagggcg cttttggcgt tgcccttgcg gtcagtgcgc aggttatgcc actcaatttg
    15841 tgatgaaaaa gtattacaca gcgtttcaac gtggcttgcc ggacgtgcat gtacgccgtt
    15901 aggtagctca caaataaatt ccacaatcag ggccattgcc tctctcccgg tacggttttc
    15961 tgtttacagg ataagagcgc acggcaacgg cctgccatgt gacaaatctg ccaaaagctg
    16021 gacaaatgta atgtaaccgt caatttgcga cgcgtctcac aagacgctgt tttgcggcat
    16081 gcttccggtt tatcgcaagt tatgaggcgg atcgcatttt tgtactgata ttacaaaagt
    16141 ccagtaaatg gcctttttat ccactgtttg ctccgcctgc gattgcctat tgtttcctcc
    16201 atcaaaaata tggacatggg ctttcgcaag cccgggagca gcttatgaat gagttggtgc
    16261 agatcctgaa aaatacccgt cagcatttaa tgacgggcgt ttcacacatg attcccttcg
    16321 tggtatcggg cggtattttg ctggcggttt ccgtcatgtt gtatggcaaa ggcgcagtgc
    16381 cggatgccgt agccgatccg aatctgaaaa aactgtttga tatcggcgtt gcaggtttga
    16441 cgctgatggt gcctttcctc gccgcttaca tcggttactc cattgcagag cgttctgcgc
    16501 tggctccgtg cgctatcggt gcctgggttg gtaacagctt tggtgcgggc ttctttggtg
    16561 cactgatcgc cgggattatc ggcggcatcg tggtgcatta cctgaagaaa attccggtgc
    16621 ataaagttct gcgctcggtg atgcctatct tcattattcc tatcgtcggc acactgatta
    16681 ccgcaggcat catgatgtgg ggcttgggcg agcctgtagg ggcgttgacc aacagcctga
    16741 ctcagtggct tcaggggatg cagcagggca gcattgttat gctggcggtg atcatgggtc
    16801 tgatgctggc gttcgatatg ggcggtccgg ttaacaaagt ggcctatgcc ttcatgctga
    16861 tttgcgttgc tcagggtgtt tataccgtgg tggccatcgc tgccgtgggt atttgtatcc
    16921 cgccgctggg gatggggctg gcgacgctga ttggtcgtaa aaatttctcc gcagaagagc
    16981 gcgaaaccgg caaagcggca ctggtgatgg gctgtgttgg cgttactgaa ggggcgattc
    17041 ctttcgccgc tgccgatccg ctgcgtgtca ttccttccat catggtcggt tcagtttgtg
    17101 gtgcagtaac tgcggcgctg gtcggtgcgc agtgctatgc aggctggggt ggtctgattg
    17161 tgctgccggt ggttgaaggc aagctgggtt atatcgcagc agtggctgtc ggagcagtgg
    17221 tgacggctgt ttgtgtgaac gtgctgaaaa gtctggcgcg taaaaatggg tcttcgactg
    17281 atgaaaaaga agacgacctg gatttggatt ttgaaattaa ttaattgagg aacgagccat
    17341 gacgaaaatt attgcagtaa ccgcatgtcc ttcaggtgtt gcccatacct atatggcagc
    17401 agaggcgctg gaaagtgccg cgaaagcgaa aggctgggaa gtgaaagtag aaacccaggg
    17461 gtcaattggt ctggaaaatg aactgactgc ggaagatgtg gcgagcgctg atatggtgat
    17521 tctgaccaaa gatatcggca tcaagtttga agagcgtttt gctggcaaga ccatcgtgcg
    17581 cgtcaacatc agcgatgcag taaaacgtgc tgacgccatt atgagcaaaa ttgaagctca
    17641 cctggcgcaa accgcttaag ttcttctccc gctcgcaagg gcgggttcgc tttcccacag
    17701 gagttcctca tgacgaatcg tatctctcgc ctcaaaactg cactgtttgc caatacccgt
    17761 gaaatctcgc tggagcgggc gctgctttat accgccagcc atcggcaaac cgaaggcgaa
    17821 ccggtgatat tgcgccgggc gaaagcaaca gcgtatatcc ttgaacatgt tgaaatttcg
    17881 attcgtgatg aagaactgat tgccggtaac cgcaccgtaa aaccgcgcgc tgggattatg
    17941 tcgccggaaa tggaccctta ctggctgctg aaagagctgg atcaattccc gacgcgtccg
    18001 caggaccgct ttgctatcag tgaagaagat aaacgtatct accgtgaaga gttgttcccg
    18061 tactgggaaa aacgttcgat gaaagatttc atcaacgggc agatgacaga tgaagtaaaa
    18121 gccgcgacca gcacgcagat tttcagcatc aaccagacag ataaaggcca ggggcacatt
    18181 attattgatt acccacgcct gctgaatcac gggctggggg agctggtggc gcagatgcag
    18241 caacattgtc agcaacagcc ggagaatcac ttttatcagg ccgcgctgtt actgctggaa
    18301 gcctcgcaga aacacattct gcgttacgct gaactggcgg aaacaatggc ggcaaactgc
    18361 acagatgccc agcgtcgcga agaactgctg actattgcgg agatctcccg ccataacgcc
    18421 gaacataagc cgcagacgtt ctggcaggcg tgccagttat tctggtacat gaacatcatt
    18481 ctgcaatacg aatccaacgc cagttcgcta tcgttggggc gcttcgacca gtatatgttg
    18541 ccgttctatc aggcatcttt aacccagggc gaagatccgg cgttcctgaa agaactactc
    18601 gaatctttat gggtgaaatg caacgacatc gtgctgttgc gctccaccag tagcgcgcgt
    18661 tatttcgccg gtttcccgac cggctatacc gcactgctcg gcgggttaac cgagaacgga
    18721 cgtagcgcgg tgaacgtgct ttcgttcctt tgccttgacg cctatcaaag cgtgcaatta
    18781 ccgcaaccga acctcggcgt gcgcactaac gccttgatcg acacgccgtt cctgatgaaa
    18841 accgccgaaa ccattcgcct cggcaccggt attccgcaaa tctttaacga tgaagtggtg
    18901 gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg cgcgcgacta ttccgtagtg
    18961 ggctgtgtgg aattatctat tcccggcaga acctacggct tgcatgacat cgcgatgttt
    19021 aatctgctga aagtgatgga aatctgcctg tatgaaaatg aaggcaatgc tgagctgact
    19081 tatgaaggtt tactggagca gatccgcgcc aagatcagcc actacatcac cctgatggtt
    19141 gagggcagca atatttgtga tatcggccat cgcgactggg cacctgtacc gctgctctcg
    19201 tcttttatca gcgattgtct ggaaaaaggc cgcgatatta ccgatggcgg cgcgcgttat
    19261 aacttctccg gcgtacaggg gatcggtatc gccaacctga gcgattctct ccatgcgttg
    19321 aaagggatgg tttttgagca acagcgttta agttttgacg aattgctgtc ggtattaaaa
    19381 gccaactttg caacgccaga aggcgaaaaa gtccgtgctc gcttaattaa ccgcttcgag
    19441 aaatacggta acgatatcga cgaggtggat aacatcagcg ccgaactgtt acgccactac
    19501 tgcaaagaag tggaaaaata ccagaacccg cgcggcggtt acttcacgcc gggatcgtat
    19561 accgtttctg ctcacgtccc gttgggatcg gtggttggcg cgacgccaga cggtcgtttt
    19621 gccggggaac aactggcaga cggcggcttg tcacctatgc tgggccagga cgcacaaggg
    19681 ccaacagcgg tactgaagtc agtcagtaag ctcgataaca cgctgctgtc taacggtacg
    19741 ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg aagcgggatt acgcaaactg
    19801 gccgacttct tacgggcgtt tacccagctt aagttgcagc atattcagtt taacgtggtg
    19861 aacgccgaca cgttgcggga agcgcaacag cgcccacaag attatgccgg gctggtggtg
    19921 cgcgttgccg gatacagcgc cttctttgtc gaactgtcga aggagatcca ggatgacatc
    19981 atccgccgga cagcgcatca gctgtaacgt tgtggaaacg cgccgcgatg atgtggcgcg
    20041 cattttcaac attcagcgtt attcactgaa tgacggcgag ggcattcgta cggtggtctt
    20101 ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat ccggagtcga tctccggcaa
    20161 aatccagacg gtacgcagag aggcgaaatg tctgcactgt gcgaaatgtt tgcgtgatgc
    20221 ggatgaatgc ccctccgggg cgtttgaacg gattggccgc gatatcagcc ttgacgcgct
    20281 ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg tccggcggcg gcgtcacgct
    20341 ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc cgttttttac agcggctgcg
    20401 gttgtggggc gtgtcatgcg ccattgaaac tgccggagac gcgccagcca gcaagctatt
    20461 accactggcg aaattgtgcg atgaagtgct gttcgattta aaaattatgg acgcgactca
    20521 ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg gagaatctgc gtttgctggt
    20581 gagtgagggt gtcaacgtga tcccgcgttt accgttgatc cctggtttca cgctcagccg
    20641 ggagaatatg cagcaggcgc tggatgtact gatcccgctg aaaatcaggc agatccatct
    20701 gttaccgttt catcagtacg gcgaaccgaa ataccgcctg ctggggaaaa catggtcgat
    20761 gaaagaggtg cctgcgccgt cgtcagccga tgtgacaacg atgcgcgaaa tggcagaacg
    20821 ggccggattt caggttaccg tgggaggtta aaatggcata cctggtggca gtaaccgcct
    20881 gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg gctggaaaag ttgtgccagt
    20941 tagagaagtg gggcgtcagc attgaaactc agggcgcgct gggaacggag aatcgtttag
    21001 cggacgagga tatccgtcgg gcggatgttg ctctgttgat tacggatatc gagcttgccg
    21061 gtgccgagcg atttgaacat tgccgctatg tgcaatgcag catctacgca ttcctgcgtg
    21121 agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc tgcgccgcag caaacccatc
    21181 ttattctgga gtagtcggtt tttctgtcag ttggctgtgg tactgccggc gatattccga
    21241 cggcgagcgt tcggtgtttt tacgaaacag acggcagaag tagttgctgt cgacaaagcc
    21301 gcaggcgtgc gccacttctt ttaccttcag gtcatagcct ttcaataacg tcttagcgtg
    21361 ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg gcccccgttt tttgaaacag
    21421 gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg acagattcgc gggtaagcgc
    21481 ggaggcatag cgttcgtcga tataatcgcg aatagcttca aatagtgcct ggctgcgtga
    21541 ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg ctaagcaggc tggctacgat
    21601 aagccttgcc gtttgctgct cctgcggctg catttgcatt tcattgagcg tttgtagcag
    21661 aaaagaacct atacgtgggc cgcgtcgggc gacgtgttgc ttcgccagat tttgatattg
    21721 tttgccatcc cattgcacga cgctgaagcc gagttgctgt ttgccaaaca gcacgctaaa
    21781 ggtagtagcg ggggcttgcc attgtggaaa attccagcca ccagcgggaa cgtacagcac
    21841 atcgccggga actaacgttg ctccagcgcc ggtatcaaca aactcacctt ccagcactat
    21901 ttccagccgt ggaaaatcga cctgatacgc aagatcgggc gccggaccgt tactgctggc
    21961 aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg cgggagagca gatagctgac
    22021 gtcgtgatac atgttattcc cttaggtatg tagacgggaa aacagtaact ggcattatta
    22081 aaaaaggcca cactgaaaag tgtggcctga ggggagttcg atgtaaacat tgcggcagta
    22141 tcgcatccgg caaatagcgc cagacgcgat acaaaggtca tttactgatt acctacctgg
    22201 tcgccatacg gcagagtgtc gtaatcgatc agcgcgtttt tcttgtacgg gttaccaatc
    22261 cagcgggtag tttctttgaa ctgcggattc accactgaac gggttggatc gtaaccgtcg
    22321 taagataagc ccgccaaatc tgaccaggtg tggatcagtt ccgccaggct gtatttacga
    22381 tcgacatcct gtgagaaatc acggggatga gtcgcttgcc atttttccga cgtccacagc
    22441 aggaacggaa tggtgtacat gtggcgcgtc gggttgtctt cgttacgccc ctgagttttg
    22501 tgcggcggcg tgtcgtaaac ctcttcaccg tggtcagaga agtaaaccag gaatccgttc
    22561 gggtctgccg ctttaaagtc tttaatcagg ctggcaacca catgatcgtt atacaggtta
    22621 gcgttgtcat aatcgttata tgattccagc tcttctgcgc ttaatcctgg cggaacatga
    22681 tcggtattgc catcaaactt gccctgattt tccgggtagc ggtatttgta tttgatatgc
    22741 gtacccagca gatgaacgat gatcagtttc ttcggcgcag ggtcattcag cacatcctgg
    22801 aacggcttca gcacgttggt gtcgtattca cgcgcactct gcgtacgttg ctggttcatg
    22861 tagtactgct tgtcggtctg gcgcgaaaat accgtcagca tggtattgcg ggcggtcatc
    22921 gtctgctggt tggtgatcca gaaggtttta taacccgcct gtttcatcat gttcatcagc
    22981 gacggctgcg tcagatacag atccgggttc ttttcattgg caaaggtcag cgcctgttgc
    23041 aggatttcaa tggtgtacgg acgagacgta actacgttat taaacacggt cagattcgga
    23101 tcggttttat gcagcgcatc cagctccggc gtggtttcac gcggataacc gtacagactc
    23161 atgcgtccgc gctgggtcga ctcgccaatc accagcacta aagtgcgcgg ttcgttaccc
    23221 gattcatctt tgaaattagc cagtggcggc aaggcattat tttcattcag taactttgtt
    23281 agcgagttta gttgctgacg atactgataa tagccggtca ggaattgcca cggtgcggca
    23341 ggctccatac gcgaggccag gttatccaac gttttctcaa acggcttgtt tttgataaac
    23401 gtattcatgg cgatcggatg cagaatcaag ccataaagca gggcaaaaga gacaatataa
    23461 cgccacggct ttggaatata gaccgggcgc agacgtgtcc acagcagaac tgccattgcc
    23521 gtataggcca gcgcgataag cacaattttc aggctgaaat actggcttaa atactcgctg
    23581 gcttcgttgg tgttggtttc gaacatcaca aacagaacgc tctgcgagaa ctcctgaccg
    23641 tagatgacgt agtagcacag cgccgccaga gaggccgccc atagcaccac gccgattact
    23701 gcggcaataa ttttaatccg cttcggaaag aggaataccg ggatcaacca cagcgaactg
    23761 aataacagcg agtcgcgaat gccgttagtg ccactataac cactgatgta aataatggcc
    23821 tgtagcagag tagagaaaaa ccaaaagtag agcagtgccc aacccagggc tttccagcta
    23881 aaaaggggtt tagcctggac ttctgtggaa tgcatagtaa gaacctgtct tgaaaaaaat
    23941 atcgccgaat gtaacgacaa ttccttaagg atatctgaag gtatattcag aatttgaata
    24001 aaatgcagac agaaatatat tgaaaacgag ggtgttagaa cagaagtatt tcagaaaacc
    24061 ctcgcgcaaa agcacgaggg tttgcagaag aggaagatta gccggtatta cgcatacctg
    24121 ccgcaatccc ggcaatagtg accattaacg cttgttcgac gcgaggatcc ggttcctggc
    24181 cttctttttc tgcctggcgg gagcggtgca gtaactcggc ctgcaatacg ttcagcgggt
    24241 cggtgtaaat attccgtagc tgaatagact ctgcaatcca cggcagatcg gccattagat
    24301 gggaatcgtt ggcaatcgcc agcaccactt tgatgtcttc ttcttgcagg ttgcgtaact
    24361 ctttacctaa cggccacagt gctttgtcta ccaggcgttg gtcatagtat tccgccagcc
    24421 acaggtctgc tttggcgaag accatctcca gcatgccgag acgcgtcgag aagaatggcc
    24481 aatcgcggca catggcttcc agctcgctct gtttgccatc ttcgaccact ttttgcagcg
    24541 ccgtacctgc acccagccag gcggggagca tcaggcggtt ttgcgtccag gcaaaaatcc
    24601 acggtatggc gcgcagcgac tcgacgccgc cggttgggcg acgtttcgcc ggacgtgaac
    24661 ccaacggcag tttgcccaat tcttgttccg gcgtagcgga gcggaagtaa ggcacaaaat
    24721 ctttgttttc acgtacgtag ccacggtaga gatcgcagga gatgactgac agttcatcca
    24781 taatgcgacg ccagctctct ttcggctcag gtggtggcag caggttggct tccagaatcg
    24841 ccccggtgta cagcgacagg ctgctgacgg tgatttctgg cagaccatat ttaaagcgga
    24901 tcatctcgcc ctgttcagtc acgcgcaggc cacctttcag gcttcctggc ggttgtgaca
    24961 gcagcgccgc atgagcaggt gcgccgccgc gaccaatgga accgccgcga ccgtggaaca
    25021 acgtcagctc aatacccgct ttttcgcagg ttttgattaa tgcatcctgt gcctgatatt
    25081 gcgcccagga agctgccatc actcccgcat cttttgctga gtcggaatag ccaatcatca
    25141 ccatctgttt gccctgaatc aggccacgat accagtcaat attgagcagc tgggtcatga
    25201 catcgttggc gttgttcaga tcatcgaggg tttcaaacag cggagcaacc ggcatcgcaa
    25261 acccgatacc cgcttctttc agcagcaggt ggacagccag tacgtcggac ggcgttttcg
    25321 ccatcgagat cacgtaggcg gcaatggagc cttgcggcgc ttcggcaatc acctggcagg
    25381 tatcgagcac ttcgcgcgtt tcggcgcttg gttgccagtt gcgcggcaga agcggacgtt
    25441 tggagttcag ttcgcggatc aggaacgcct gtttgtcggc ctctgaccag ctttcgtagt
    25501 cgccgatacc gaggtagcgg gtcagctcgc ccagcgcttc ggtatggcgg gtgctctcct
    25561 gacggatatc aatacggacc agcggtacgc cgaaacattt cacgcggcgc agggtgtcga
    25621 gcagatcgcc gttggcgata atacccatgc cacacgcctg aagtgactgg tagcaagcgt
    25681 agagcggttc ccacagttct tcgttttgtg tcagcagccc ttctggtttt ggcagttctt
    25741 cgcctttcag gcgcgcttcc agccatgcct gtgtcgccat caggcgagaa cgcaggtttt
    25801 tcatcagata gcgatacggt tctgcggcac cttcttcgcc aaccagcgcc agcagttcag
    25861 gggtcgcttc aaccatcgac agttcagaaa ccagcacctg aatatctttc aggaacagat
    25921 cggtggcttt ccaacggctg agcagcagga cgtggcgggt gatatcggca gtgacgtttg
    25981 ggttgccgtc gcggtcaccg cccatccacg aggtaaaacg taccggaaca aattcgacgg
    26041 gcagtttgta gccgaggttc tcttccagtt gttcgttcag ttcgcgcagg taattcggta
    26101 cgccttgcca caggctgttt tctactacgg caaagcccca tttggcttca tctaccgggc
    26161 ttggacgcag cttacggatt tcatcggtat gccatgactg ggcgattaac tggcgcaggc
    26221 gacgcatcag ctggttgtgt tcgtagtcag cgatatcttt gttatcgagc tgttttaagc
    26281 aggcgttcac ttccaccatt ttgtggatca gtgtacgacg ggtaatttcg gtagggtgag
    26341 ccgtgaggac cagttccagc gacagcgatt ccactgcttt tttgatggtg tcttcgctca
    26401 gttccggctg gtttttcagt ttacgcaggg tgcgggcgat cacttccggg ttgctggcag
    26461 cttcgccttt cggcgaaatg ctgtggtatt gctcggcggt gttggccagg ttcaggaact
    26521 gactaaacgc acgcgcaacg ggcagcagct cgtcgttcga caaattttgt aaggtggtga
    26581 gtaactcctg gcggttagca tcattgccag cgcgtgaaga tttggacaac ttacggatag
    26641 tttctacgcg ttcaagaatg tgttctccca acgcatcctt gatggtttct cccagcactt
    26701 tgccgagcat actgacatta ctacgcaatg cggaatattg ttcgttcata ttaccccaga
    26761 caccccatct tatcgtttga tagccctgta tccttcacgt cgcattggcg cgaatatgct
    26821 cgggctttgc ttttcgtcgt cttttataaa gccacgtaaa agcggtgacg tcaaatgctg
    26881 cgaaatcgct tcagcaaacg aataaatagc aggaatttac gtcattaaat tcacgacgct
    26941 ttaaataagc gtaacttatg gaaatgttaa aaaatcgccc caagtaacac caaaggtgta
    27001 ggtcggataa gatgcgcaag tatcgcatcc gacattattg cggcactgga gtttgacaac
    27061 agtgccggat gcggcgcgag cgccttatcc ggcctacgtt ttaatgccag caaaaatggt
    27121 gaattacctg ggttatcagt tcgcgggtgg gcttgataaa ccgtgtttcc agatattcat
    27181 caggttgatg agcctgatta attgagccag gccccaacac cagcgtcggg cataacgttt
    27241 gaataaacgg cgcttcggta cagtagttca ccacttcggt ctttgctccg agcaatttct
    27301 caactacttc aaccagttga tgattcggtg ggcattcata gccagggatc ggcggatgca
    27361 gctcgtcgac cgtcagacga cccggccagc gttcgctcac cggagccaat gcatcgttga
    27421 gcaaaccatt aagttcattg agtgtcatgc caggcagcgg acgaatatcc atatgcaact
    27481 cacagcaagc gcaaatacgg ttagaagcgt cgccaccgtg aatatgcccg aggttgagcg
    27541 tagggtatgg cacggtaaac gcttcgtagt gataacgttc tttcaggtta tcgcgcaatt
    27601 gcaaaatatg cccgatggcg tcgtgcatta gttcgatagc gttaactccg cgtgctggat
    27661 cgctggagtg ccccgactgg ccctgaatac ggatggcgtt agagatatga cctttatgtg
    27721 cgcgtaccgg ttgtagtgac gtcggttcgc caatgatggc gcaatccggg cgcagggcgg
    27781 tagtttcggc aaaataacgc gctccggcca tactggtttc ttcatcagca gtcgccagaa
    27841 tgtagagcgg ttttttcagt ttcgtgacgt cgacatcgcg tagcgcatca aggataaacg
    27901 caaaaaagcc tttcatgtcg gcggtgccta agccgtaaag cttgccgtca tgctccgtca
    27961 gtgtaaacgg atcgcgcgtc cagcgaccgt catcaaatgg caccgtatcg gtatgccccg
    28021 ccagcaacaa gccgccagcc ccctgtccga tacttgccag catattaaat ttgttgcgag
    28081 ttcctggaac aggctgcact tccacattga agcccaaatc tttaaaccag tccgccagca
    28141 gagtgattaa atctgcattg ctttgatcga gtgcctcttc cgtggcgctt attgaaggtg
    28201 tggcaatcag agcgcggtaa atctcgataa atggcggtaa tttgtttttc attgttgaca
    28261 cacctctggt catgatagta tcaatattca tgcagtattt atgaataaaa atacactaac
    28321 gttgagcgta ataaaaccca ccagccgtaa ggtgaatgtt ttacgtttaa cctggcaacc
    28381 agacataaga aggtgaatag ccccgatgtt gaatacgctg attgtgggtg ccagcggcta
    28441 cgctggcgca gagctagtga cctatgtaaa tcgccatccg catatgaaca taaccgcttt
    28501 gactgtttca gcgcaaagca atgatgcggg aaagttaatc tccgatttgc atccgcagct
    28561 aaaaggcatc gttgatctgc cgttgcagcc gatgtcggat atcagcgagt ttagccctgg
    28621 ggtggacgta gtgtttctcg ccaccgccca tgaagttagc cacgatttag cgccgcagtt
    28681 tcttgaagcg ggctgcgtgg tgttcgacct ttccggcgcg tttcgtgtta acgacgccac
    28741 cttctatgaa aaatattacg gctttaccca tcaatacccg gaactgttgg aacaggcagc
    28801 ctacggtctg gcggagtggt gcggtaataa attaaaagaa gcgaatttga ttgcggtgcc
    28861 gggctgttat ccgacggcgg cacagctggc gctgaaaccg ttgattgatg ccgatcttct
    28921 tgacctcaat cagtggccgg tgatcaacgc caccagcggc gtgagcggtg cagggcgtaa
    28981 agcggccatt tcaaacagct tttgtgaagt tagcctgcaa ccgtatggcg tctttactca
    29041 tcgccatcaa ccagagatcg ccacacacct cggtgctgac gttatcttca ccccacatct
    29101 gggcaatttc ccgcgcggca ttctcgaaac cattacctgc cgcctgaaat cgggtgtgac
    29161 ccaggcgcaa gtcgcgcaag tgttacagca ggcgtatgcc cataaaccgc tggtgcggct
    29221 gtatgacaaa ggcgttccgg cgctgaaaaa tgtcgttggg ctgccatttt gcgatatcgg
    29281 gtttgccgtt cagggcgagc atctgattat tgtggcgacc gaagacaact tactgaaagg
    29341 cgcggcggca caagcggtac agtgcgccaa tattcgtttc ggctatgcgg aaacgcagtc
    29401 tcttatttaa gggtgcaatg atgaatccat taattatcaa actgggcggc gtactgctgg
    29461 atagtgaaga ggcgctggaa cgtctgttta gcgcactggt gaattatcgt gagtcacatc
    29521 agcgtccgct ggtgattgtg cacggcggcg gttgcgtggt ggatgagctg atgaaagggc
    29581 tgaatctgcc ggtgaaaaag aaaaacggcc tgcgggtgac gcctgctgat cagatagaca
    29641 ttatcaccgg agcactggcg ggaacggcaa ataaaaccct gttggcatgg gcgaagaaac
    29701 atcagattgc ggccgtaggt ttgtttctcg gtgacggcga cagcgtcaaa gtgacccagc
    29761 ttgatgaaga gttaggtcat gttggactgg cgcagccagg ttcgcctaag cttatcaact
    29821 ccttgctgga gaacggttat ctgccggtgg tcagctccat tggcgtaaca gacgaagggc
    29881 aactgatgaa cgtcaatgcc gaccaggcgg caacggcgct ggcggcaacg ctgggcgcgg
    29941 atctgatttt gctctccgac gtcagcggca ttctcgacgg caaagggcaa cgcattgccg
    30001 aaatgaccgc cgcgaaagca gaacaactga ttgagcaggg cattattact gacggcatga
    30061 tagtgaaagt gaacgcggcg ctggatgcgg cccgcacgct gggccgtccg gtagatatcg
    30121 cctcctggcg tcatgcggag cagcttccgg cactgtttaa cggtatgccg atgggtacgc
    30181 ggattttagc ttaagttttg ttggccggag gcgcagcttt ccggcattga atttcaaaat
    30241 aaggaaacag agttatggca ctttggggcg ggcgttttac ccaggcagca gatcaacggt
    30301 tcaaacaatt caacgactca ctgcgctttg attaccgtct ggcggagcag gatattgttg
    30361 gctctgtggc ctggtccaaa gccctggtaa cggtcggcgt gttaaccgca gaagagcagg
    30421 cgcaactgga agaggcgctg aacgtgttgc tggaagatgt tcgcgccagg ccacaacaaa
    30481 tccttgaaag cgacgccgaa gatatccata gctgggtgga aggcaaactg atcgacaaag
    30541 tgggccagtt aggcaaaaag ctgcataccg ggcgtagccg taatgatcag gtagcgacag
    30601 acctgaaact gtggtgcaaa gataccgttg gcgagttgct gacggctaac cggcagctgc
    30661 aatcggcgct ggtggaaacc gcacaaaaca atcaggacgc ggtgatgcca ggttacactc
    30721 acctgcaacg cgcccagccg gtgacgttcg cgcactggtg cctggcctat gttgagatgc
    30781 tggcgcgtga tgaaagccgc ttgcaggatg cgcttaagcg tctggatgtc agcccattag
    30841 gctgtggcgc gctggcggga acggcctatg aaatcgaccg tgaacagtta gcaggctggc
    30901 tgggctttgc ttcggcgacc cgtaacagtc tggacagcgt ttctgaccgt gaccatgtgt
    30961 tggaactgct gtcggctgcc gctatcggca tggtgcatct gtcgcgtttt gctgaagatc
    31021 tgattttctt taacaccggc gaagcggggt ttgtggagct ttctgaccgc gtgacttccg
    31081 gttcatcatt aatgccgcag aagaaaaacc cggatgcgct ggagctgatt cgcggtaaat
    31141 gcggtcgggt gcagggggcg ttaaccggca tgatgatgac gctgaaaggt ttgccgctgg
    31201 cttacaacaa agatatgcag gaagacaaag aaggtctgtt cgacgcgctc gatacctggc
    31261 tggactgcct gcatatggcg gcgctggtgc tggacggcat tcaggtgaaa cgtccacgtt
    31321 gccaggaagc ggctcagcag ggttacgcca acgccaccga actggcggat tatctggtgg
    31381 cgaaaggcgt accgttccgc gaggcgcacc atattgttgg tgaagcggtg gtggaagcca
    31441 ttcgtcaggg caaaccgctg gaagatctgc cgctcgacga gttgcagaaa ttcagcccgg
    31501 tgattgacga agatgtctat ccgattctgt cgctgcaatc gtgcctcgac aagcgtgcgg
    31561 caaaaggcgg cgtctcaccg cagcaggtgg cgcaggcaat tgcttttgcg caggcgcggt
    31621 tagagtaatt tgtttatatt gctcccctga aaatggggag cttaccacgc ctacaaagtg
    31681 cgacacttcg taggcggata aggcgtttac gccacatcag gcagaaattt gtcagcattc
    31741 tggtgagctt aaatttaacg cccaaccaga accattattt gtcacattcc tttttatcat
    31801 tgtgtcttct ttaccttcac actccccata aaaatcatat tttcaataca tcaatcaata
    31861 catattttaa ttaattgaat ataattcatt tctattgttg tgcttatttt taatgattaa
    31921 tggagtttgt tatgtgatta tttaacgtgt tgttttaacg atggtagtta attattcagg
    31981 tggtttttga gggtattttt tctcctcggc ggaaattttt atttttaatg tgatcacggt
    32041 tgaataatcg ctctttctct cttgtatggt agtaggcgta aaattaagtt tgaagcgtct
    32101 atttacaaca caggagattg caataatgaa aattattgct gcagatgttt ttgttacttg
    32161 cccagggcgt aatttcgtta cgcttcaaat aaccactgaa agcggacttt gtggattagg
    32221 agatgccacg ttaaatggtc gcgaattgtc ggtcgcttct tatctgaaag atcatctttg
    32281 tccacaacta attggccgcg atgccagccg tatcgaagat atctggcaat tcttttataa
    32341 aggtgcttac tggcgacgtg gccccgtcac gatgtcagcc atttctgcca ttgatatggc
    32401 gttgtgggat atcaaagcta aagcggcaaa tatgccattg tatcaactgc ttggtggtgc
    32461 ttctcgtgaa ggcgtgatgg tttattgcca caccaccggg cggacgattg atgaagtcct
    32521 tgaagactat gcaaagcacc agcagatggg ctttaaggcg attcgtgtgc agtgcggtgt
    32581 accgggtatg caaactactt atggcctggc gaagggcaaa ggcctggcgt atgaaccggc
    32641 aaccaaaggc ctgtggccag aagagcagtt gtggtcttca gaaaagtatc tcgattttac
    32701 gccgaagtta tttgaagcgg tgcgtaataa gttcggtttt aacgaacatc tgttgcatga
    32761 catgcaccat cgcctgaccc ccatcgaagc ggcgcgcttt gggaagagca ttgaggatta
    32821 ccgcctgttc tggatggaag atccgacgcc tgcagagaac caggaatgtt ttcgtctgat
    32881 tcgccagcat accgtgacgc cgattgcggt cggtgaagtg tttaacagta tctgggactg
    32941 caagcagtta atcgaagagc agctgattga ctatattcgc acgacgatca cccatgcagg
    33001 cggcattacc gggatgcggc ggattgccga ttttgcatcg ctttatcagg tacgtaccgg
    33061 ttcgcatggc ccttcagatt tatcgcctgt ttgtcatgcg gcggcgctgc attttgacct
    33121 atgggtacca aattttggcg ttcaggagta catgggctat tccgaacaaa tgcttgaagt
    33181 tttcccgcac agctggcgct tcgaggaagg ttatatgcac ccaggcgatg aaccggggct
    33241 gggtatttct ttcgatgaga aactggcggc gaaatatccg tatgacccgg cgtatctccc
    33301 cgtcgcacga ctggaagatg gcacattgtg gaactggtaa taacggttcg cttaattatt
    33361 ttctgaatag tcatcaaatc gattaattcc gctcaaggag ctaaaaatgg acgttgatgt
    33421 gtcaaccagt gttgcgggta ataaaccgca acggattcgt cgtattcaga cagtgaccct
    33481 ggttttatta tttatggcgg ggatcgttaa ttttctcgac cgttcgtcat tgagcgtggc
    33541 aggtgaagcg attcgtggcg agttaggatt atcggcgaca gagtttggtg ttttgctttc
    33601 tgcattttct ctgtcttatg gtttttcaca actaccttcc ggtattttgc tagaccgctt
    33661 tggcccacga attgttttag gcgcaggctt aatattctgg tcattaatgc aggcattaac
    33721 aggaatggtt aattctttta gccactttat tttaatgcgt atcggtctgg gtattggcga
    33781 agcgccattt atgcctgcgg gagtaaagtc gatcaccgac tggtatgcac aaaaagaacg
    33841 cggcacggcg ctggggattt ttaactcatc taccgttatc ggtcaggcta tcgcgcctcc
    33901 tgctcttgta ttgatgcagc tggcatgggg ctggcggacg atgttcgtta tcatcggcgt
    33961 ggcagggatt ctggttggga tctgttggta cgcgtggtat cgcaaccggg cgcagtttgt
    34021 cctgactgac gaagaacgga cgtatctctc cgcgccggtt aaaccgcgtc cacagctgca
    34081 atttagcgag tggctggcgc tgtttaagca tcggacaacc tgggggatga ttttaggttt
    34141 ctctggcgtc aattataccg gttggctcta catcgcgtgg ttacccggtt atttgcaagc
    34201 cgagcaaggt ttcagtctgg cgaaaaccgg ttgggtggcg gcgattcctt tcctcgcggc
    34261 ggcagtcggg atgtgggtta acggtattgt tgtcgatcga ctggcgaaaa aaggctacga
    34321 cctggcgaag acgcgtaaaa cggctattgt ctgcggtttg atgatgtcgg cattaggcac
    34381 gttgctggtc gtgcaatctt cctcgccagc ccaggcggta gcgtttatct caatggcgct
    34441 attctgcgtg catttcgctg gaacgtctgc atgggggctg gtgcaggtga tggtgtcaga
    34501 aacaaaagtg gcttccatcg ccgggattca aaactttggc agttttgttt ttgcttcctt
    34561 tgctccgatc gtaaccggtt gggtagtgga taccacacac tcgtttaatc tggcgttggt
    34621 tatcgcggcc tgtgtgacgt tcaccggggc gctgtgttac ttctttattg tcaaagatcg
    34681 cattgagtaa tcgttactat caggctctct tgctgtgggc ctgtagaata aaaaaaagcg
    34741 gatcctggag atccgcaaaa gttcacgttg gctttagtta ttcgagttga gaaactctcg
    34801 aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc
    34861 agtcagaatg cttgataggg ataatcgttc attgctattc tacctatcgc catgaactat
    34921 cgtggcaatg gaggatggat aatgaatatt cgtgatcttg agtacctggt ggcattggcc
    34981 gaacaccgcc attttcggcg tgcggcagat tcctgccacg ttagccagcc gacgcttagc
    35041 gggcaaattc gtaagctgga agatgagttg ggcgtgatgt tgctggagcg gaccagccgt
    35101 aaagtgttgt tcacccaggc gggaatgctg ctggtagatc aggcgcgtac cgtactgcgt
    35161 gaggtgaaag tccttaaaga gatggcaagc cagcagggcg agacgatgtc cgggccgctg
    35221 cacattggtt tgattcccac agttggaccg tacctgctac cgcatattat ccctatgctg
    35281 caccagacct ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg
    35341 gcgcaactgg acagcggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa
    35401 gcattcattg aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagatcac
    35461 ccgtgggcga accgcgaatg cgtaccgatg gccgatctgg caggggaaaa actgctgatg
    35521 ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt tctgttttga agccggggcg
    35581 gatgaagata cacacttccg cgcgaccagc ctggagacgc tgcgcaacat ggtggcggca
    35641 ggtagcggga tcactttact gcctgcgctg gctgtgccgc cggagcgcaa acgcgatggg
    35701 gttgtttatc tgccgtgcat taagccggaa ccacgccgca ctattggcct ggtttatcgt
    35761 cctggctcac cgctgcgcag ccgctatgag cagctggcag aggccatccg cgcacgaatg
    35821 gatggccatt tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg cagctacccg
    35881 ataggcttcc gccatggtcg ggtagttaaa ggtggtgttg acgaagtact caatagtgtt
    35941 gccgccacct ttctgttcca taatcgcctg accgatatga ataatttcgg cagcgcgctc
    36001 gccaaagcag tgaataccca gaatctcttt tgtttcccga tggaacaaaa ttttcagcgt
    36061 gcccacgttc atgccgacga tttgtgcgcg tgccagatgt ttaaactggg cgcggcccac
    36121 ttcatatggc actttcatcg cggtcagctg ctgttcggtt ttgcccacag agctgatttc
    36181 cgggatggtg taaatgccgg tagggatatc ttcaatcaga tgcgcggtgg cttcgccttt
    36241 taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca ggctcggata
    36301 accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca tgctgttgac
    36361 cttcagctgt ccgcgactgt cagtttccag cccaatgttc tgtaacgcca gcgaatcggt
    36421 attaccggta cgaccgttgg catagagcag gcagtcagct ttcagttttt tacccgactt
    36481 cagatgcatg atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt cgttgtgacg
    36541 aatcactacg ccactgttcc agaagtgata ggagagagaa tctgacatct cttgatcgag
    36601 aaatgccagc aggcgatcgc gggtgttgat cagatccact tttacatcca taccgcggaa
    36661 gatcgacgca tattcacagc cgatcactcc agcaccatag ataagtacat ggcgcggttc
    36721 gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga aatcaacatc
    36781 tgttggatga tatggacgag agccgcaggc aataacaaat ttttcagcgg ttagtgtttc
    36841 aacgctgccg tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc gagcgtttcc
    36901 ctgcaatatt tcacagtgat tacgttcgta aaatccctga cgcatgcgcg tttgttgatt
    36961 aatcacgtta tcggcatggt taaggatatc ggcaaaagaa gagcggagca gtcgggaatg
    37021 gtcgctgtaa agtgggtttt gattgaattc tataatgcgg ctgacggcgt gacggagagc
    37081 tttcgacggg atggtgcccc agtgggtgca accgccgcca acattttgat aacgctcgat
    37141 aactgcgacg cgcgcacctt gcttaaccag gcccattgca gcgccttcgc cgccggggcc
    37201 ggaacctatt actatggcat cgtaatcgta ggaatgtggc atggtagggc ttacctgttc
    37261 ttatacataa aagcaacaga atggtaacat tttatcgcgg gtaagccaat tgatccccgt
    37321 catttatctg gctatatcct gagcggcctt tgctttgtct gtttcttact tttgccctga
    37381 cgttttattg gatttttatc gacgatactc tccgtttaag cggcaggttt ccgctgtacg
    37441 taaaagaacc ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca
    37501 atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
    37561 gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg
    37621 gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct gagtttgcgt
    37681 gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt ccgcgacgta
    37741 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgc
    37801 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
    37861 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
    37921 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
    37981 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
    38041 gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa
    38101 cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa aggggcttat
    38161 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
    38221 taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt gctggcttat
    38281 cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct gtattcccga
    38341 ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat cgcaccatgc
    38401 cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg ctgtacagca
    38461 cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca gtactctccg
    38521 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt
    38581 aatcggtatt atgccggatg ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat
    38641 acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag acgttcgacc
    38701 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccggatt gcaggagata
    38761 tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
    38821 ggtgggtcga caaaaatggt ttcgcactga taactcttca agtctatccc ttgcaggcga
    38881 ttaaactcgc gtacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
    38941 tgtacgttat caatatggtt agctgcgatg ttgtattgcg cagcagcaac cgacggctta
    39001 gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gtgctaatga aaagttaccg
    39061 ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat
    39121 tccagcatct gaatattcat cgccgcgttc ggctgggtaa aactgttttc tacctgacgg
    39181 tagatcatct ctttccctgc gaccggcaga cgttcatcga tgtaatcttg atccagctcg
    39241 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca
    39301 tcgcgcaggg cttccgcttc ctgacgccac tcatcatcca gctttttatg gtacaacagg
    39361 gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc
    39421 agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gatgagttca
    39481 ctggcagcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc gaaaatgatg
    39541 tgatacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg gtaatgactg
    39601 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
    39661 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
    39721 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
    39781 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
    39841 gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
    39901 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
    39961 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc
    40021 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
    40081 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttctgtca cagccttttc
    40141 cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca
    40201 gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata tcgaccgctg
    40261 gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa
    40321 cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt
    40381 ggtgttaatt gatggcgtac gcctgaatct ggcgggggtg agtggttctg ccgaccttag
    40441 ccagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt
    40501 ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg atgaacccgg
    40561 aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacgca
    40621 gcaacaactg ggggataaga cacgggtaac gctgttgggc gattatgccc atactcatgg
    40681 ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg cagacagata acgatggttt
    40741 tttaagtaaa acgctttatg gcgcgctgga gcataacttt actgatgcct ggagcggctt
    40801 tgtgcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt ctcccggttc
    40861 accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc tgcgctataa
    40921 cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag attacaacta
    40981 cgatccccat tttggtcgtt atgattcgtc ggcgacgctc gatgagatga agcaatacac
    41041 cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg
    41101 gcagaaacag actacgacgc cgggtacagg ttatgttgag aatggatatg atcaacgtaa
    41161 taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg aaggcgcagc
    41221 acgcagtgac gataactcac agtttggtcg tcatggaacc tggcaaacca gcgccggttg
    41281 ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata aggcaccaaa
    41341 tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga aaagcaaaca
    41401 gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt ccggatatcg
    41461 taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt acaacgaagg
    41521 gaaagcgcgg attaagggcg tcgaggcgac cgccaatttt gataccggac cactgacgca
    41581 tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc cgttgttacg
    41641 ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
    41701 tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt atccttatca
    41761 aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc
    41821 tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg agacagtcta
    41881 tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac
    41941 gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc
    42001 ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct tttccgtatg
    42061 gcgaaaaaag cgaagagttt attgttgagc gagtggtggc aattgtcacc gcagtgcaag
    42121 aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc gtttcacttc
    42181 ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg
    42241 ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga actgttaaac
    42301 gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg
    42361 gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat gtttctctgg
    42421 atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg
    42481 tattgggttg cacccatttc cctctactac aagaagaact cttacaggtg ctgccagagg
    42541 gaacccggct ggtggattct ggcgcagcga ttgctcgcag aacggcctgg ctgttagaac
    42601 atgaagctcc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc
    42661 cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac
    42721 tggcagtttt aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc
    42781 tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa
    42841 cacgccgccg ggtcagcggg gttctcctga gaa
//
LOCUS       DAFAVF010000021        42476 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFAVF010000021 DAFAVF010000000
VERSION     DAFAVF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.021"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            124..1344
                     /locus_tag="LGK42_001138"
     CDS             124..1344
                     /locus_tag="LGK42_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBK2657412.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1317..1946
                     /locus_tag="LGK42_001139"
     CDS             1317..1946
                     /locus_tag="LGK42_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBK2657413.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRITATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1947..3107)
                     /gene="ybdL"
                     /locus_tag="LGK42_001140"
     CDS             complement(1947..3107)
                     /gene="ybdL"
                     /locus_tag="LGK42_001140"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBK2657414.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGASQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3216..4304
                     /gene="hcxA"
                     /locus_tag="LGK42_001141"
     CDS             3216..4304
                     /gene="hcxA"
                     /locus_tag="LGK42_001141"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBK2657415.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4314..4511)
                     /locus_tag="LGK42_001142"
     CDS             complement(4314..4511)
                     /locus_tag="LGK42_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBK2657416.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4693..6798)
                     /gene="cstA"
                     /locus_tag="LGK42_001143"
     CDS             complement(4693..6798)
                     /gene="cstA"
                     /locus_tag="LGK42_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBK2657417.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6979..7392)
                     /gene="entH"
                     /locus_tag="LGK42_001144"
     CDS             complement(6979..7392)
                     /gene="entH"
                     /locus_tag="LGK42_001144"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBK2657418.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7395..8141)
                     /gene="entA"
                     /locus_tag="LGK42_001145"
     CDS             complement(7395..8141)
                     /gene="entA"
                     /locus_tag="LGK42_001145"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBK2657419.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8141..8998)
                     /gene="entB"
                     /locus_tag="LGK42_001146"
     CDS             complement(8141..8998)
                     /gene="entB"
                     /locus_tag="LGK42_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBK2657420.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9012..10622)
                     /gene="entE"
                     /locus_tag="LGK42_001147"
     CDS             complement(9012..10622)
                     /gene="entE"
                     /locus_tag="LGK42_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBK2657421.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10632..11807)
                     /gene="entC"
                     /locus_tag="LGK42_001148"
     CDS             complement(10632..11807)
                     /gene="entC"
                     /locus_tag="LGK42_001148"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBK2657422.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12182..13138
                     /gene="fepB"
                     /locus_tag="LGK42_001149"
     CDS             12182..13138
                     /gene="fepB"
                     /locus_tag="LGK42_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK2657423.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13142..14392)
                     /gene="entS"
                     /locus_tag="LGK42_001150"
     CDS             complement(13142..14392)
                     /gene="entS"
                     /locus_tag="LGK42_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBK2657424.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14503..15507
                     /gene="fepD"
                     /locus_tag="LGK42_001151"
     CDS             14503..15507
                     /gene="fepD"
                     /locus_tag="LGK42_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBK2657425.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15504..16496
                     /gene="fepG"
                     /locus_tag="LGK42_001152"
     CDS             15504..16496
                     /gene="fepG"
                     /locus_tag="LGK42_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBK2657426.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16493..17308
                     /gene="fepC"
                     /locus_tag="LGK42_001153"
     CDS             16493..17308
                     /gene="fepC"
                     /locus_tag="LGK42_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2657427.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17305..18438)
                     /gene="fepE"
                     /locus_tag="LGK42_001154"
     CDS             complement(17305..18438)
                     /gene="fepE"
                     /locus_tag="LGK42_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBK2657428.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18654..22535)
                     /gene="entF"
                     /locus_tag="LGK42_001155"
     CDS             complement(18654..22535)
                     /gene="entF"
                     /locus_tag="LGK42_001155"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBK2657429.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQVLML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22532..22750)
                     /gene="ybdZ"
                     /locus_tag="LGK42_001156"
     CDS             complement(22532..22750)
                     /gene="ybdZ"
                     /locus_tag="LGK42_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBK2657430.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22753..23955)
                     /gene="fes"
                     /locus_tag="LGK42_001157"
     CDS             complement(22753..23955)
                     /gene="fes"
                     /locus_tag="LGK42_001157"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBK2657431.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24198..26438
                     /gene="fepA"
                     /locus_tag="LGK42_001158"
     CDS             24198..26438
                     /gene="fepA"
                     /locus_tag="LGK42_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBK2657432.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26613..27233
                     /gene="entD"
                     /locus_tag="LGK42_001159"
     CDS             26613..27233
                     /gene="entD"
                     /locus_tag="LGK42_001159"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBK2657433.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27355..27507)
                     /gene="hokE"
                     /locus_tag="LGK42_001160"
     CDS             complement(27355..27507)
                     /gene="hokE"
                     /locus_tag="LGK42_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBK2657434.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27858..28010)
                     /gene="hokE"
                     /locus_tag="LGK42_001161"
     CDS             complement(27858..28010)
                     /gene="hokE"
                     /locus_tag="LGK42_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBK2657435.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            28452..29570
                     /locus_tag="LGK42_001162"
     CDS             28452..29570
                     /locus_tag="LGK42_001162"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBK2657436.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29636..29884
                     /locus_tag="LGK42_001163"
     CDS             29636..29884
                     /locus_tag="LGK42_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBK2657437.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29949..30317
                     /locus_tag="LGK42_001164"
     CDS             29949..30317
                     /locus_tag="LGK42_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK2657438.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30411..31064
                     /gene="nfsB"
                     /locus_tag="LGK42_001165"
     CDS             30411..31064
                     /gene="nfsB"
                     /locus_tag="LGK42_001165"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBK2657439.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31172..32419
                     /gene="ybdG"
                     /locus_tag="LGK42_001166"
     CDS             31172..32419
                     /gene="ybdG"
                     /locus_tag="LGK42_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBK2657440.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32487..33863)
                     /gene="pheP"
                     /locus_tag="LGK42_001167"
     CDS             complement(32487..33863)
                     /gene="pheP"
                     /locus_tag="LGK42_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBK2657441.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33965..37108)
                     /gene="cusA"
                     /locus_tag="LGK42_001168"
     CDS             complement(33965..37108)
                     /gene="cusA"
                     /locus_tag="LGK42_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBK2657442.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVTALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37120..38343)
                     /gene="cusB"
                     /locus_tag="LGK42_001169"
     CDS             complement(37120..38343)
                     /gene="cusB"
                     /locus_tag="LGK42_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBK2657443.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38359..38691)
                     /gene="cusF"
                     /locus_tag="LGK42_001170"
     CDS             complement(38359..38691)
                     /gene="cusF"
                     /locus_tag="LGK42_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBK2657444.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38715..40097)
                     /gene="cusC"
                     /locus_tag="LGK42_001171"
     CDS             complement(38715..40097)
                     /gene="cusC"
                     /locus_tag="LGK42_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBK2657445.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40254..40937
                     /gene="cusR"
                     /locus_tag="LGK42_001172"
     CDS             40254..40937
                     /gene="cusR"
                     /locus_tag="LGK42_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBK2657446.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40927..42375
                     /gene="cusS"
                     /locus_tag="LGK42_001173"
     CDS             40927..42375
                     /gene="cusS"
                     /locus_tag="LGK42_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBK2657447.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10118 a        11786 c        10877 g         9695 t
ORIGIN      
        1 taactttcat ctatttattc acagcgcgaa tgaaaaataa aacactctgc tttgcagtgt
       61 ttactttata attaacagac ttatctcact gatcaccctg taacgttcag agagcgtctt
      121 cagatgtcta tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt
      181 attacctgga cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaagac
      241 tctggtttaa tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc
      301 gttttgttta tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg
      361 cgcgagttgt acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg
      421 caaaattccc tttcacaatt ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg
      481 gtgcgtcagc ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcct
      541 ggcatgacct tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg
      601 gcggcgatga tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc
      661 agtttaaata aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt
      721 catagctggt acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat
      781 gcaaatcatc aaagtctctg caacccactg tataacctaa tgtatcaggc aggcgttcct
      841 ctgcgtcata tgcgaatttg cgaacctttt ggcccggagc aacgacaagg attatggctc
      901 tatcacgtta tcgaaccgga tcgctgggca gctatgtgcg cacgagtcag cggggtaaaa
      961 agtggcggca tttacgccgg acatgacaat catttctatg ggcatcggaa aatcctcaag
     1021 ccagaacatt tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa
     1081 acagctgagc attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc
     1141 atcgaggttc cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg
     1201 cggatatgca aagttttact caataacgat tactggtgtc gagcattatc atttagtcct
     1261 acgaaagcga agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg
     1321 gggatactat gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg
     1381 aagatgatcg catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct
     1441 ttcgtgaaga gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg
     1501 attacaaccc aaacaacgtg gcaccacccg aaaaaaaact gctgaaaaaa tccattgaaa
     1561 tagatggttt tacccaaccc attgtggtca cgcatacgga taaaaatgcc ctggaaatcg
     1621 tggatggttt tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa
     1681 aaggttattt gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc accgcgacta
     1741 ttcgtcataa tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg
     1801 aacttagcca gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg
     1861 acgaagtgct gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat
     1921 attcccgcgc ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc
     1981 aacgtcgatt ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg
     2041 caaaacaccg acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac
     2101 tcaacatcat ccagggtaga aacctcgctg taatccacca gcaaaaagta tgtaccttca
     2161 cacggtaaaa tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg
     2221 cgataaaagt ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc
     2281 gccagctgtg ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg
     2341 gcgctgatgg gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc
     2401 ttgccaaatg aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg
     2461 gcatggccct gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc
     2521 tcgtgcccgg cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gatagttgca
     2581 ctggggttat gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca
     2641 aattcctgcc agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc
     2701 ccagaaagcg cgatggcggg ggagtaactg tcatagctgg gatcaaaaca aatcacttca
     2761 tcgccattgc gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt
     2821 acggtgatat cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca
     2881 atcgcctcgc gcaaggcctg cacgccggtc atgggcgcgt attggcttgc cccctgggca
     2941 acgtggtacg ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc
     3001 gacaggttaa tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg
     3061 ccaagttgtg gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct
     3121 gacgaagttg cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt
     3181 tagatgtcta aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg
     3241 tcgtcggccc ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt
     3301 tcactgatga acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg
     3361 cgcaaaccaa acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg
     3421 gtcattgcag cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg
     3481 tggtgattgg cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc
     3541 tcggtctgcc atttgttgcc gctccgacga tcgccgccac ctgcgcagcc tggacaccgc
     3601 tctccgtctg gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca
     3661 attttatggt gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg
     3721 cggggatcgg tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag
     3781 aaacgttgcc gctaaccgtg cggctgggga tcaataatgc gcaggccatt cgcgacgtct
     3841 tgttaaacag tagcgaacag gcacttgccg atcagcaaaa tcagcagtta acgcaatcat
     3901 tttgcgatgt ggtggatgcg attatcgccg ggggtggaat ggtcggtggt ctgggcgatc
     3961 gttttacgcg tgtggctgcg gcccatgccg tgcataacgg tctgactgtc ctgccgcaaa
     4021 ccgagaaatt tctccacggc accaaagtcg cttacggaat tctggtgcaa agcgccttgc
     4081 tgggtcagga tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga
     4141 ctacgctggc ggagctggaa gtggatatca ataatcaggc ggagatcgac aaagtgattg
     4201 cccacacctt gcgcccggtg gagtccattc attacctgcc agtcacgctg acaccagata
     4261 cgttgcgtgc agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag
     4321 cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg
     4381 taggtcatcg gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa
     4441 tcaggcatac caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt
     4501 gaatcaaaca tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga
     4561 acgctttatc cggcctacaa atgctatcaa cgcactcggt cagtcccctc gccccatcgg
     4621 ggagagggtt agggtgaggg ggaaccctcg gcactatgcc aacattcgcc aacatccccc
     4681 ctcactctga ctttagtgcg cacctttcgc ctgcgctacg atctcctcaa cattttccgg
     4741 cattggctca tacggcgttt ctttcgccgt tggcttcggc tctttcaatg ccgcaagtgc
     4801 cgtcttaatc gagaacagtg ccagaaccac cacgaccacc ataaagaaga tggttaaccc
     4861 ggcatccaga cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg
     4921 aatattgccg ctgtcgatca ttgcctggaa cttgttagca atggccagga agccgacttt
     4981 cgcatccggg ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gtagccaggc
     5041 cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc
     5101 acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag
     5161 agtgttaata ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag
     5221 tacgcacagc gccgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg
     5281 agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac
     5341 cgccgtcaga ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc
     5401 catcatacca cccagcgcac cgtgcaggat gtaagccatc cccaccgcca acgtcggcgc
     5461 accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt
     5521 atctggtgta atgctaaagc cccagctact caccacctgc gcggcagaag cgaccacatc
     5581 cgccgtccct gccggagcca gcaccgccat tgggctgttc atggcaaagt aaacgcccgg
     5641 atcgatgata caggcggaaa ccagcgccat aatcgccacg aaggattcca tcaacattcc
     5701 accgtagccg ataaaacaag cctgcccttc gttcgccagc atcttcggcg tggtgccgga
     5761 agagatcagc gcatggaagc cagacaccgc gccacaggcg atggtgataa acaggaacgg
     5821 gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat
     5881 ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga ccgacgatcg tcccgatttt
     5941 caggaaggta gagaggtagt cacgcggggc cagcagcaac cacaccggca gcaccgccgc
     6001 cacaaaaccg taacccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg
     6061 tgcccaggtc ggactttctg ccacccagcc gccagagata atggcgaaaa tcaggaatac
     6121 cagaccgatg accgacactt caccaatacg ccccggacgc agatagcgca ggtagatccc
     6181 cataaacagc gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt
     6241 cagggctttc accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca
     6301 ggccaccagc gctatcaccc cggcggttgg ccccatctct tctttgacca gctcacccag
     6361 cgatcgaccg tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc
     6421 gagaaccacc ccggcgagca gccagatcat ccctggcagg taacccattt gcgccgccag
     6481 taccggccca accaacggac ctgctccggc aatggccgca aaatggtgac caaacagcac
     6541 tttcttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg
     6601 cgtcgggtca accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat
     6661 cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt
     6721 taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta
     6781 tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagatatg ttacattttg
     6841 tttctataac atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac
     6901 caatatttaa cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct
     6961 catttaattc tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg
     7021 ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc caagatgcag
     7081 cggctggcag acgccgcgta ccttgccttc agacaccggg cgatgatggg tggcgttaag
     7141 ttccgtaccc accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag
     7201 cgtttcagcc agcgccgctg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg
     7261 ggtatcaacc ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc
     7321 cagatgcgcc accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg
     7381 tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg
     7441 gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga
     7501 cgggcgattt tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc
     7561 tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca
     7621 ggcgaaacca cattacagcg cacgccgcta cccgccagtt ccagcccgac gctcaacgcc
     7681 aggcttttca gcgccgcttt cgatgcgcca taagcactca tgccaatacg cggcgtgtgc
     7741 gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg
     7801 gtttgctgga acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc
     7861 tctttactga gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg
     7921 tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg
     7981 gcaacatcca tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa
     8041 ccggtaactt tcgctcccgc ctcaacaaac gccagcgccg tggcgtagcc gataccttta
     8101 cctgcgccgg ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga
     8161 gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcac
     8221 catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt
     8281 cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct
     8341 cacgcagcgc cgctttgctg gcggggacag gtgctggcag taattcttca gtcatcacca
     8401 cccggccaga ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat
     8461 cggccagcgc atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg
     8521 tggtcataca gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct
     8581 ctttgagcat ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca
     8641 ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg
     8701 tcaggcccgg cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg
     8761 gctgggcggt gtaataaacc ggaatattgt gctgtttgca gtagtcgcgc agcgcagcaa
     8821 tattcgcgat cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt
     8881 cctgcatatc atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat
     8941 tcttagggat atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg
     9001 tgttctctcc ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg
     9061 actttcccga ccgccgtcag cggaagtgaa tctacacact ccacgcgatc cggtaattta
     9121 aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct
     9181 tttaccacca gataagcgca gcttttttcg cccatcagct catcttccat gctcaccagt
     9241 gcggcgtaga tcaccgccgg gtggcgcagc agcaggtttt cgatctcttc ggcagcgatc
     9301 ttctcaccgc cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtacccctct
     9361 ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca
     9421 ttgtgctgcg gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt
     9481 ccaacttccc cttgcggcag tggatttcct tcggcatcgg caacccacac ttcatcgtcc
     9541 ggacacattg ggtaaccctg ggtatggata attttctccg cgctatcatc aagacgggtg
     9601 tagttcacca gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg
     9661 ggaatacgcg ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc
     9721 gaggcaagct gcgcccggct ttcaccttcg gtcagcgcct gcaaccacag gctgactgcc
     9781 ggtggcacca gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa gcaaagcgtg
     9841 gcgctgggat cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatccc
     9901 ggcgaactca tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt
     9961 gtgaactgac aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc
    10021 gggatcagtt tcggtgtgcc ggtggtgccg ccggaaagct ggaaataggc cacttcatca
    10081 gcaggtgatg gcgtggcagt aaaatcctca gccggatggt taatcgcatc ctgcaagtta
    10141 tgctcaccgc tgtcgttgtg cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa
    10201 gtattgagga aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagcaatgcg
    10261 ggttcaatct ggctggcata ggcgttcagt tcactacgct gatggctgaa cagcgccagc
    10321 accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa tatacaattc agcgacgtta
    10381 cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgtaa actacacgcg
    10441 aggttatccg ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc
    10501 gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag
    10561 tagccttttt cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc
    10621 atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt
    10681 tcgcgccact cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc
    10741 acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg
    10801 ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaatggctc cagttcagca
    10861 ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg cgggggttgg atgcagcaga
    10921 caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga
    10981 tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg
    11041 cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt
    11101 tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc
    11161 gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc
    11221 gggctggccc ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc
    11281 gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg
    11341 atatcaatca atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg
    11401 gcgcgggcaa ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg
    11461 ttcagcgact ggctgcgggt aaaacggcgg gctgaggtct gtttttcctg gcgggagaac
    11521 gactgccagg attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc
    11581 ccgaccatca ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt
    11641 ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg
    11701 aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca
    11761 agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca
    11821 aaatgataaa ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca
    11881 aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt
    11941 gtggttacaa tgaaaatgag aagcattatt gatggattcg cataagcgca atgtgatgtc
    12001 ctgcgccgtt ctgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg
    12061 caccagtgct ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat
    12121 tgtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgatt
    12181 tgtgagactc gccccgctct accgcaacgc ccttctatta acaggacttt tactttcagg
    12241 aatagccgca gttcaggccg ctgactggcc gcgtcagatt actgacagcc gtggcacaca
    12301 tacactggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggctcact
    12361 gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc
    12421 ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg
    12481 gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt
    12541 aattagcgca accggcgggg attcggcgct ggcactgtat gatcagcttt ccaccatcgc
    12601 cccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg
    12661 cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact
    12721 ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac
    12781 cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga
    12841 acaactcggc tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg
    12901 caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaatggcga
    12961 gtcactattc ctgttcgccg gtgatcagaa agatgccgat gcgatttatg ccaatccact
    13021 gctcgcgcac cttcccgcag tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt
    13081 ccgtctggat tactacagcg ccacgcaagt gctggaacga cttaaggcgc tgttttaagc
    13141 attaactgtc agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca
    13201 gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgcttgcg cttgcggaag
    13261 caaccggtgt catcatcgcg cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg
    13321 tcacgttctg cgccgtccac aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg
    13381 tttgcagcat tgtatattgc agcaacgaac taaccgcgct cagccagccg aacagcgcca
    13441 gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg gcgaggaacg
    13501 atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc agcttcccgc
    13561 tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg
    13621 ccgctgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc gcgctcgcca
    13681 tcgtcaataa accacccagc agcgcaatcc cgcccaccag cgggctggca agcagaaaac
    13741 gaaatcctgc cagtaatgat ttcaacggat gctcacgcgg ctgcggtggc ggtggcaacg
    13801 ccggaaggct taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc agcccgtagt
    13861 tccaggctac gccaccggtc gctagcaata aaccgccaat catgggcgaa atcaccgacc
    13921 ccagacgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca cgccctacca
    13981 gtgctggtgt cgccgccaat agcgccgtaa cgccaagcga tgcgaaaaaa ccatcccata
    14041 aaccaagtaa atagattgcc agcaatgacg gctccggcag cagtgcatta aggcacagtc
    14101 caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgctcatagc
    14161 gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg gtcagcgtca
    14221 ccgaaagccc cacctgccag gtagaatgcg tcatcatctg gatctgcacc ggcaccgcga
    14281 cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact gcgcgaaacg
    14341 ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc
    14401 cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata
    14461 agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc
    14521 gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta
    14581 agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc cctctccggc
    14641 acctgccaga gcgccgactg caccatcgtg ctcgacgcgc gactgccgcg cacccttgcc
    14701 ggtttacttg caggcggcgc gcttggcctt gccggggcgt tgatgcaaac cctcacccga
    14761 aatccacttg ccgaccccgg cttgcttggc gtgaatgccg gagccagctt tgccattgtg
    14821 ctgggtgcgg cgctgtttgg ttactcatcc gcgcaggaac aactggcgat ggccttcgcc
    14881 ggggcgctgg tggcctcatt gattgttgcc tttaccggca gtcagggcgg cgggcagtta
    14941 agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga aggactgacc
    15001 agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt
    15061 tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt tctgatcgcc
    15121 ggagcaactg cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg cagtgacacc
    15181 gcgacggcgc tgggcagtcg cgtggcgcgc acacagttaa ttggtctgct ggcgattacc
    15241 gtgctttgtg gtagtgcgac agcggtagtt ggcccgattg cctttattgg cctgatgatg
    15301 ccgcatatgg cgcgttggct ggtgggtgcc gatcatcgct ggtcgctgcc cgtcacgcta
    15361 ctcgccaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc
    15421 gagttgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat cttcctcgtg
    15481 cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt
    15541 tgctgcttat ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca
    15601 cgctggaaac ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc agtatgacga
    15661 tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcggcac
    15721 tgggcgtcag cggtgcgatt tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg
    15781 taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc
    15841 aggacctgac ggctatcgcg ctgtcagcaa tggtgggcgg cattgtcact tcgcttctgg
    15901 tctggctgct cgcctggcgt aacggcatcg acacctttcg gttgattatt atcggtatcg
    15961 gtgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg tctttagaaa
    16021 cggcgctaac agcgggtttg tggaatgccg gatcgctcaa cggcctgacg tgggcaaaaa
    16081 cctcgccttc cgcacccatc attatattga tgctcattgc cgccaccttg ctggtgcggc
    16141 gaatgcgctt gctggagatg ggcgatgata ccgcctgcgc gctgggcgtc agcgtcgaac
    16201 gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca acggcgcttg
    16261 ccgggccgat ttcctttatt gctttagtcg cgccgcacat tgcccgacgc attagcggca
    16321 ccgctcgctg ggggctgacc caggcggcgc tgtgcggagc gctgttactg ctggcggccg
    16381 atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca
    16441 gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa aaatgaccga
    16501 atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat ataccgttgc
    16561 ggaaaatctg actgtagaaa ttcctgatgg tcacttcacg gcaattatcg gaccaaatgg
    16621 ctgtggtaaa tccacgttac tgcgtacctt aagccgcctg atgacgcctg ctcacgggca
    16681 tgtctggctg gatggcgagc acattcaaca ttacgccagt aaagaagttg cacgccggat
    16741 tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg agctggtggc
    16801 gcgtggacgt tatccgcatc aaccgctgtt tacccgctgg cgcaaagagg atgaagaagc
    16861 ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg gcagatcaaa gcgtggatac
    16921 cctttctggc ggacaacgcc agcgcgcgtg gatcgcgatg gtgctggccc aggaaacggc
    16981 aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatctact
    17041 ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg tgctgcacga
    17101 tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag ggaaaattgt
    17161 tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct
    17221 gcgatgcatg atcattgacg atccggtagc cggaacgccg cttgtggtgc cgctcggacg
    17281 aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt
    17341 tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag
    17401 cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg
    17461 gcttaactga tatttaaacg gcgtaaaatt cacatcgttg atatttgctt ttgttaactg
    17521 ctcaacaaga tactgacgat tacgtaattc accgttcagt tccgcaacgt cagtgaccgc
    17581 tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg
    17641 gtcatcttta acggcctgac cattactgta tacgggcttt ttaattcccg ccgcgttggc
    17701 aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt tcgttttaat
    17761 gcgatcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt tattacggac
    17821 gttttctatc gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac
    17881 ggtctgcgcc tctttactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga
    17941 cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc
    18001 aacaatagcg cgatgcaaat ccagttcatc gattttcgcc tcttttaatt ggtccatcac
    18061 ataaggtgat gaacgcaggt actcttccag caagctaacc gactgaaact tcttgataaa
    18121 caggttaaat gcttctgtac gatcaatttt gatatccaga tccagcacac gaagcttagt
    18181 gaatgttttc tccaactctt gccactgaac aggttctgga ggcgtgacaa ccgccgcgct
    18241 ggtccatttt tgcggcagga tgaaagagat cagcaagcct gcgcaggcaa acgcaaaaac
    18301 gaccgccatg accgtttttt tggcccgcca taaaactgag attagattaa gtaaatcaat
    18361 ttcattatta ctgggcgacg ccagaggata atcgggaaaa tgagcgtcac ttccctgttt
    18421 aatattcagt gatgacatgg ggtataacct gaaaatgagt ccaatagggg gaaatctcca
    18481 cggcgctcac tttaccagtt ggtgagaatt atccgaagct gaagtttgta aattccttcc
    18541 aatgaatgca acattcactc tatttaagag aaataaccag caaacttata aattaaacaa
    18601 atgattaacc cataattatt tcctgtaatt atgggttaat agataatgtt aatttacctg
    18661 tttagcgttg cgcgaataat cggcccaatt ttttcaaacg cccctggaga gataatatcc
    18721 acatgcgcac aatcctgacg atagatatcc agctccgcta tccacggcga ccaggcgcgt
    18781 tcgggactca taccttcctg aagtgtgcgt tcagcaacaa acagcgtcgc tttaccgtca
    18841 aacggtacgc tatgagcagt cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg
    18901 gtggtaaaca actccgttga agtacttccc tgctgtgctg ccaggaaggc ctcgcgttcg
    18961 cggttaatct ccgccagcac ttccgggtcc agaccattag cttctttttc ctgccagttt
    19021 tgcgtttctg gcggccaggt atccagcaag ccaagaaatg ccacctgttc gccacgggca
    19081 cgcagtcgcg ccgcaatacc ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa
    19141 taagggccgt gcggttgttg ctcaagtaac gttgccagat gcgcttcgca gacttcatcc
    19201 aggtttgtcg ctgtctgcat gggtccatga gggcgcggcg actgaatgcc aataatcgac
    19261 cattgtggat cgagataacg cgagagcacg ctaaactgcc aggcaaaacc ggacgccgga
    19321 tggaaacaaa acagcgtcgg gccattacct tcacgcaacg gcagaatggt ttcgaatccc
    19381 atgcgccggg agctgtcctc ttcaccatca ataatcgttg ccagtttggc gacggttgac
    19441 gcgaccatca cctgccccgg cgtcacctga cgagcaaact gtcgacttaa ctgcgctgct
    19501 agtttcattg ccagtagcga atgaccgccc agcgcaaaga aatcagcatc ggcatcctgc
    19561 acgtcacaac ccagcaacga cgcgaatgcc gcggcgataa tcgtttcact gcccgctttc
    19621 ggtgcacgtc ccgacgcttg tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc
    19681 ttgccgttgg cgctaagcgg caactgcgga agttgcagca gaacgaccgg caccatatgc
    19741 ggcggcaatg tttcgcgaag ctgtgcctgt aatgcgctgg tatccaacgg cagtcccgat
    19801 tgcgacacca gatagcccac caactgacgc gcatcaccac cggtggctgc cgcctggtta
    19861 atcacacagg cgtgggtaac tgcctgttcg acatccggca gcgcctgcat cacgcgatcg
    19921 atttcgccca gttcgatacg ctgcccgcga atttttagct gatcgtcact gcgcccgagg
    19981 tactccaccg cgccgttatc cagccagcgg gcgacgtctc cggtacgata catccgttca
    20041 cctggggcaa aaggatcggc aataaaacgg ctggcggtca gatcgggtcg tccaagatac
    20101 ccctgcgcca gttggatgcc ggtgagatag agatcacccg ccacacccgg cggcaccgga
    20161 tgcatcatcg catcaagaat acgcagaccc gtattccata ccggataacc aatcggcaca
    20221 ctgctgccgc gcacctgtgc cagttcctcg ccaaaagccg gataccagct gacatctacc
    20281 gccgcttccg tcgggccata tagattatgc aacggcgcgc cagttaactg ttgccattcg
    20341 cggcataaat cagccggtaa ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa
    20401 ctctggcgag cggtttgcgg cgtcagcgag gcaacaaatg ccgccagcat cgacggcaca
    20461 aagtgcgtgg tcgttacgcc atattcggca aagaattgct gcatagcgag cgggtcgcgg
    20521 tgcgcttccg gttcagccat caccagtttc gccccggcaa taaacggcca gaaaaactcc
    20581 cacaccgaga catcaaaact gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc
    20641 gggtaatggt tttgcatcca aagcaagcgg ttaacgatag ccgtctgccc gaccattacc
    20701 cctttcggcc tgccggtgga gccagaggta aagatgatat aagccgtgtg gtgcggttgt
    20761 gaaagttgca gcggcgcact gccctggggg gtaagcgggg cgttatagca aaggcttgtt
    20821 aaattgggaa catcgctaaa gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga
    20881 cgcgcatctt ccagcatcat tttcaggcga tcgtccggat agccggtatc cagcggtagc
    20941 caggccgcgc cagcttcaac tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt
    21001 agtgccaccg ccacgctgtc ccctggttta acgccgcgct cacgcagcag attcgccagc
    21061 gccaccacct gctcgcgcat ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc
    21121 ggagcatccg gtgtttttgc cgcttgttct gccaccagcg cgctcagcgt ggtttctgga
    21181 atctcaacct gagtggcgtt gatctgcgcc agctgcgcat actcacctgg cagcataata
    21241 tcgacatcgc cgcacaacag cgccggatcc gcggcgaact gggcaatcag cattttcagg
    21301 cgttcagcat gctggattaa cgttggctca tcgtaacgct gtttattggc gaggatctca
    21361 atactcaaat caccgtgttc atccgggaac agggccagtt caaggtcatt aaccggaccg
    21421 gttgccaggg tatgggtttg cgtctgaaca ccaggaatat ccagttggta atcaaatacc
    21481 ttgatattga gtaccggacc gaacagcggt tcctctcctg ccgctcgccc gctgtcacgg
    21541 acaatttgtt cggcatcgta acgttgatga cgacgcattt ttttcagttg tgctgccagt
    21601 cgggttgcca gttccggcag cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg
    21661 ttgagcacgg gtccgctagc cgtcagcgcc gccgagccca gtcgacgcat aaagataaat
    21721 ccggcggcat agtccatgcg attgcacaat cgccccagcc acaaggctgc cagcgcaagg
    21781 gctaaatcgg tacgctgcac acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg
    21841 gtaaattcca gtttcaggcg cagaatatct gccgaagcgc tgcgccccgc taaaggtgcc
    21901 ggagaaagtg acgcgggcgg cggcagttga cgacgctgtt ctgcccagaa tgccgcatca
    21961 cgctgccagg cttcgctttc gcggtattgc tggtactctt ccactacatc agcgaaaggc
    22021 gtaaacggcg aagcaggcgt tggttcgcca cgcagccatg tgcagtaaat attggcgatc
    22081 tggcgagtaa tggccgggaa actgaagcca tcgaccagca aatggtgata acgctgatac
    22141 cagtaccagc ggttatccgc aacatgtatc agctgatgaa agaccagtgg tttaccgcta
    22201 tcgacgcgca aatcttgttg caaatccgcc agcattaata cctgcgcagt accgtgcgga
    22261 tcaatattgg ttcgcaggtc gataatttct ggcagttcga atatcagcgc atcatcgacc
    22321 cactgccaga cttcgccgtt atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct
    22381 agtccgacaa ccaccgcgcg ggccagtaat ggcgcatcaa catctccagt taactcaacg
    22441 taatgcgcca cgctccaggc ggagggtaaa tccgacagtt tttctgccat ccagatgccg
    22501 ggctgtgcgg cgaccaaagg taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat
    22561 tggccggtgt cagagtacgc cagtggactt ccagccactg ctggcaggac gcctgtgact
    22621 gcggctgaca cacaatgtcc cagcctgccg gtaaggcgca ttgttgcggc cacagactga
    22681 attgcccctg cgcattgcgc aaaatgtaaa acgctccctg tggatcatcg aagggattac
    22741 tgaatgccat attcaactcc tgtcatggaa aagtggttgc cagaggtcga ttagcccctg
    22801 catcaagcca ccgcgccaac aaagcgcatc atgtccgccg tcaacctgac gccagaaaat
    22861 ggattctttt atggggtgta attgcgcata cagcgcctga ttggcccgca taatcatcgg
    22921 ctcgcgaata cccgcttcca gcacaatgcg cagaccttcg gcgctaactt caccagcttt
    22981 aagcttttca agtaacacgc cctcttgctg cccgccccga tgcggccacc agtacgatcc
    23041 tgactggctt aatacacagc caaagcgttc aggccagtgc agtccggcat acagcgcgga
    23101 cagcccacca aaactctgcc cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc
    23161 aatagctttc accaggggta ataactcttg ctgtactgcc agccagaaat ccgcattaca
    23221 cggcagttcg tgggcgcggt gcgtggtgtc gatagcgtcg atcaacacat acacggcggg
    23281 aggaagttgc tgacgatggg tcagcgaagt cagcacaggc cagacgggca tactttgcgc
    23341 ccaaaattcg ccatcgagca aaactgccag cggacgttct tctgctgttg catcgccggt
    23401 ggtaaaaatc catacacgac gtgaattttt caaccgttca cttttccaga taatttcttt
    23461 ggctgctatt tctggcgctt gcggacaatc ccatcccggt tgcagaggcg cttgcggcat
    23521 ttcgagtgca gaaacagcgt gccctcgccc gcctttccag ctttgtggat tcagcggatc
    23581 ggctattgcc tggggtaata gttttcgcca gccttcacgt aattcgaggc gatcggggga
    23641 tggtgcagaa aaaatgtcat cgcgttcggt gggaataaag cagtagctgc cgcgccagtt
    23701 ggcattgagt tgtgtcgtcc actgccagac atcagtacct gcaattcgct gcatcgactg
    23761 gggctggctg ttctgatgat gatcggtcac accagtgatg tagatccata cgcgctttat
    23821 cgtcgagtat tcttcagaac cttggggatc acgccaccag aaagtgacct caaacatttc
    23881 gtcattcaga cgctgccatt ccgggccatg tttcgactgc caccagctct cacttcctac
    23941 ttttaacgcc gtcacaccat aaccccatgt ttactgtgca atttttcatt gattgcagaa
    24001 atatattgat aatattattg ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg
    24061 ggaagcgcgg acatttatta accaactgca ctgcgtgtct ttcaggatca aaggttttcg
    24121 cggtagcggg atgcgtcgtg ttgaggacga ccatgcccga cagttgcaat tcgtggcaaa
    24181 aatgcaggaa taaaacaatg aacaagaaga ttcattccct ggccttgttg gtcaatctgg
    24241 ggatttatgg ggtagcgcag gcacaagagc cgaccgatac tcctgtttca catgacgata
    24301 ccattgtcgt taccgccgcc gagcagaact tacaggcgcc tggcgtttcg accatcaccg
    24361 cagatgaaat ccgcaaaaac cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc
    24421 caggcgttaa cctgaccggt aactccacca gtggtcagcg tgggaataac cgacagattg
    24481 atattcgcgg tatgggtccg gaaaacacgc tgattttgat tgacggcaag ccggtaagca
    24541 gccgtaactc ggtgcgtcag ggctggcgtg gcgagcgcga tacccgtggt gatacctcct
    24601 gggtgccacc tgaaatgatt gaacgtattg aagttctgcg tggtccggca gctgcgcgtt
    24661 atggcaacgg cgcggcgggc ggcgtggtta acatcattac caaaaaaggc agcggtgagt
    24721 ggcacggctc ctgggacgct tatttcaatg caccagaaca taaagaggaa ggtgccacta
    24781 aacgcaccaa cttcagcctg accggtccgc tgggcgacga attcagcttc cgcttgtatg
    24841 gcaacctcga caaaacccag gctgacgcgt gggatatcaa ccagggccat cagtccgcgc
    24901 gtgccggaac gtatgccacg acgttaccag ccgggcgcga aggggtgatc aataaagata
    24961 ttaacggcgt ggtgcgctgg gacttcgcac cattgcaatc gctggaactg gaagcgggtt
    25021 acagccgcca gggtaacctg tatgcgggcg atacccagaa caccaactct gacgcttaca
    25081 ctcgctcgaa atatggcgat gaaaccaacc gcctgtatcg ccagaactac gcgctgacct
    25141 ggaacggtgg ctgggataac ggcgtgacca ccagcaactg ggtgcagtat gaacacaccc
    25201 gtaactcgcg tattccggaa ggtctggcgg gcggtaccga agggaaattt aacgaaaaag
    25261 cgacacagga tttcgtcgat atcgatctcg atgacgtgat gctgcacagc gaagttaacc
    25321 tgccgattga tttcctcgtt aaccagacgc tgacgctggg tacggagtgg aatcagcaac
    25381 ggatgaagga cttgagttcc aatacccagg cgctgaccgg ggcgaacacc ggcggcgcta
    25441 ttgatggtgt gagtgccacc gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt
    25501 ttgccgaaaa caacatggag ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg
    25561 atcatcacag tattgtcggc aataactgga gcccggcgct gaacatatcg caaggtttag
    25621 gcgatgactt cacgctcaaa atgggcattg cccgtgctta taaagcaccg agcctgtacc
    25681 agactaaccc gaactacatt ctctacagta aaggtcaggg ctgctatgcc agcgcgggcg
    25741 gctgctatct gcaaggtaac gatgacctga aagcagaaac cagcatcaac aaagagattg
    25801 gtctggagtt caaacgcgac ggctggctgg cgggcgtcac ctggttccgt aacgattatc
    25861 gcaataagat tgaagcgggc tatgtggctg tagggcaaaa cgcagtcggc accgatctct
    25921 atcagtggga taacgtgccg aaagcagtgg ttgaaggtct ggaaggatcg ttaaacgtac
    25981 cagttagcga aacggtgatg tggaccaata acatcactta tatgctgaag agtgaaaaca
    26041 aaaccacggg cgaccgtttg tcgatcatcc cggagtacac gttgaactca acgctgagct
    26101 ggcaggcgcg ggaagatttg tcgatgcaaa cgaccttcac ctggtacggt aagcagcagc
    26161 cgaaaaaata caactacaaa ggtcagccag cggttggccc ggaaaccaaa gaaatcagtc
    26221 cttacagcat tgtgggtctg agcgcgacct gggatgtgac gaagaatgtc agtctgaccg
    26281 gcggcgtgga caacctgttc gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg
    26341 gcgatctggc aggggccaac tatatcgcgg gtgccggggc gtatacctat aacgagccgg
    26401 gacgtacgtg gtacatgagc gtaaacactc acttctgatg ctaacgtcag attgttgaca
    26461 aagtgcgcgt cgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaaatctt
    26521 gccaattcaa tatattgcag gaccatgtag gcctgataag cgtagcgcat caggcagttt
    26581 agcgtttgtc atcagtctct aatatggtcg acatgaaaac tacgcatacc tccctcccct
    26641 ttgccggaca tacgctgcat tttgttgagt tcgatccggc gaatttttgt gagcaggatt
    26701 tactctggct gccgcattac gcgcaactgc aacacgctgg acggaagcgc aaagccgaac
    26761 atttagccgg gcggatcgct gcaatttatg cgctgcggga atatggctat aaatgtgtgc
    26821 ccgcaatcgg cgagctacgc caaccggtgt ggcctgcgga ggtatacggc agtattagcc
    26881 actgcggggc tacggcattg gccgtggttt cccgtcaacc gattggcgtt gatatcgaag
    26941 aaattttttc tgcgcaaaca gcgaccgaat tgacagacaa cattattaca ccagcagaac
    27001 acgagcgact cgcagactgc ggtttagcct tttctctggc gctgacactg gcattttccg
    27061 ccaaagagag cgcatttaag gcaagtgaga tccaaactga tgcaggtttt ctggactatc
    27121 agataattag ctggaataaa cagcaggtca tcattcatcg tgagaatgag atgtttgctg
    27181 tgcactggca gataaaagaa aaaatagtca taactctgtg ccaacacgat taattgacaa
    27241 catctggtac gattcgcccg cagccatcac tgaccgcggg cgaaagtgta aagcaggtgc
    27301 cttaccactc tgacctgaca accggatatg cggggattgc tccccgcaca acggctactt
    27361 cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttta cggtgaactc
    27421 acacagcgag tctccgacca gaagcgtaaa tcccagtacc gtcaaacaca gcactatgac
    27481 tgccacaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac
    27541 catccgagtt gttgaggctc ggagtggcag ccccaggatt gatagaaata tctcctgggg
    27601 cttcatcttt ccagacctca gagttaacct gaaaccggaa agcttcaggc acccgccgtt
    27661 atcttaccct tcctcaacaa aatagctgtt tatttataca gtgaatcctg tttcggactt
    27721 ctgtcggcaa aactcatcat tgctcttaaa acgaaatcgc ccgcagccat cactgaccgc
    27781 gggcgaaagt gtaaagcagg tgccttacca tcctgacctg acaaccggat atgcggggat
    27841 tgctccccgc acaacggcta cttcttcggt tcgtaagcga gaacaacctt aaactcaata
    27901 ttacgttcct ttacggtgaa ctcacacagc gaatccccga ccagaagcgt aaatcccagc
    27961 accgttaaac acagcactat gactgccgca agggcatatt tcgtcagcat gtttatattg
    28021 cctccatgcg aagaagggac taccatccaa attgttcagg tttgggtagg tagccccagg
    28081 gttgatagaa atatcgtcct ggggcttcat ctttccagac ctcagagtaa acctgaaacc
    28141 ggaaagcttc aggcacccgc agttatctta cccttgctta gcaaaatagc tgtttattta
    28201 tacagtaagt cctgtttcgg acttctcact ccccatttga tcaactgaag ctgaacccgt
    28261 ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc agccagccgt gccataactt
    28321 gtcacattgc aaaagtagcc aaattgcttc agtcaccatc accaaaaaaa gaaacattcc
    28381 agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa acgccaactt
    28441 aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc ctcggtattg
    28501 aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac tcttcaatgc
    28561 tgattgacgc ggttaaaaat gagatcacgg ccggagaggt aaagcacgat atcaccgaaa
    28621 gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc gggcagtttt
    28681 cagcgatgca gaaagtcgta ttgcaggcag ccgcagacca tcatctggaa atttgcggcg
    28741 gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgaaaac gaacgctatc
    28801 aacgaacgct ggaaaacttt ggctatctca tccagcaggc gaccgttttt ggtcagcatg
    28861 tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc ctgtcacggt
    28921 ttgtgccgca cttcatcgcc ctttccgccg cgtcgccgta tatgcaggga acggatacgc
    28981 gttttgcctc ctcacgaccg aatatttttt ccgcctttcc cgataatggc ccgatgccgt
    29041 gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg cctgagttac accacgatga
    29101 tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt ggcacggtgg
    29161 aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg gcgggattaa
    29221 ttcaggccac cgcccactgg ttactgacgg aacgcccgtt taaacatcag gagaaagatt
    29281 acctgctgta taaattcaac cgtttccagg cctgccgcta tgggctggaa ggcgtcatta
    29341 ccgatccgca cactggcgat cgtcgaccgc taacggaaga taccttgcga ttgctggaaa
    29401 aaatcgcccc ttccgcacat aaaatgggtg catcgagcgc gattgaggcc ctgcatcgcc
    29461 aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc gatggtggct
    29521 cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa gtagggaatt
    29581 gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca atgttatgaa
    29641 acacccttta gaaaccttga ctaccgcagc aggcattttg ctgatggctt tcctctcttg
    29701 cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg tgaccatgtt
    29761 ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt ttttagtgct
    29821 gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct cgctggcgga
    29881 ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct tcgcctaaag
    29941 gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc cttgagttac
    30001 cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa attggcggca
    30061 aaatttttat gctgtcgtcg gagctgcgcg gcgtcccctt tatcaatctg aagtccgatc
    30121 cacaaaaatc cctgttaaat cagcagatat acccgagcat taagccaagg tatcacatga
    30181 ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg ttacttcgcg
    30241 atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc gatcaaaaaa
    30301 gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac catttctcgt
    30361 tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt atggatatca
    30421 tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa aaacttaccc
    30481 cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc accaactccc
    30541 agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt gccaaatccg
    30601 ctgccggtaa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac gtcgtggtgt
    30661 tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac caggaagatg
    30721 ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt cgcaagttct
    30781 tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca aaacaggttt
    30841 atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac gcggtaccca
    30901 tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag aaaggctaca
    30961 ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac gctacgctgc
    31021 cgaaatctcg tctgccgcaa aacataacct taaccgaagt gtaattctct cttgccgggc
    31081 atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt tcagccgtca
    31141 tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc aggttgaaga
    31201 tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg gtattatttt
    31261 tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga ccttcgaaaa
    31321 acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata aactcttcca
    31381 ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat tctggctgca
    31441 aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga tcatgatgta
    31501 tgcgctgctt tcagtcttct cgttgctgga tgtgattttg aatctggcgc agaaattccc
    31561 ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga tcggcgcgat
    31621 tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga ttctgatcag
    31681 cggtcttggt gcaatggctg ccgtgctgat gttggttttt aaagatccga ttcttggtct
    31741 ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact ggctggagat
    31801 gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg tcaaagtgcg
    31861 taactgggac aataccatta ccactattcc cacctggtct ctggtttctg actcctttaa
    31921 aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta tcagtattga
    31981 tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt
    32041 gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc gccagcaagg
    32101 ttctacggag tcgatattaa acctgcgccg aatgaccaat attggaacct ttcgtgccta
    32161 tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgactt taatggtacg
    32221 ccaactggct ccaggtgata acggtttacc gctcgagatc tatgcgttta ccaacaccgt
    32281 ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat ttgcgattgt
    32341 cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc gctctctggc
    32401 gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag agcggacatc
    32461 gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa tgccacaaac
    32521 aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc catcgtgcac
    32581 atcagcagca aaatcatgcc gaggaaggca atgcaaagat agttgccgaa cggatagagc
    32641 agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ctgcacgaaa acgcagatgc
    32701 gccagacaga tcataatcca gttcaacagc agcgttgcta ccaccagcgc catcagcaga
    32761 ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt gatcgctccg
    32821 gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa aaacttcggc
    32881 gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac cccgctgtta
    32941 tacactgaca gcgatgctac cagaatgacg aagttcagcg cagaagctac cacgttgctg
    33001 tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc cacccacgga
    33061 tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca gcaggatgcg atacaccacc
    33121 tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc agtaatccca
    33181 atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat cagcccattc
    33241 cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc tttctcgccg
    33301 ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat cgccagcact
    33361 ttaatcaacg caaaccagaa ctcggtttcg ccatataagc gcacgttcac caggttaacg
    33421 gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc cgggaaccag
    33481 tactgcatat agatgcccgc agcggtcagc tctgccatcc ccaccagcac gaacatcacc
    33541 cagtagttcc agccagagag gaagcccgcg aacggtcccc agtatttata ggcaaagtgg
    33601 gcaaacgaac cggataccgg ctcttcgacc accatctcgc cgagctggcg cataatcagg
    33661 aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc catctgaatc
    33721 gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc aatcagttga
    33781 atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc agtatcttcc
    33841 gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt gaggggcacc
    33901 ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac ggggtatcct
    33961 gcttttattt ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa
    34021 taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg gcaatccggc
    34081 tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg gcgataatca
    34141 ccgccaccgt catcgctttc gggcgcacgc gcagtaccgc gccgtgatac aacgcctcat
    34201 ccagcttctg ctcactgaat gtttgcggat tattcaacga cggctcggcc tctatggcgt
    34261 gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa
    34321 agccagtacc cgtcgccaca gaaagatgaa agcccatcca ccacagcaac cagatgccgc
    34381 ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg
    34441 ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat
    34501 ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta
    34561 actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat
    34621 cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg
    34681 gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg
    34741 tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca
    34801 gattaatggg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc ccgcccaccg
    34861 cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgcgcg gctttttcac
    34921 ggttaatctc aacgttgata tagcgcccac cttccagacg ctcggcaagc gcagaagcca
    34981 cgcctggtac cgttcgtgcc acctcttcaa tttgctccgc catcgcgtcg atatccgcca
    35041 gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccagtcgag agcatatcga
    35101 tacggttacg aattggcggc acccacagat tcgccagccc cggcagccgc acggtgttat
    35161 ccagttcctc aatgattttg tccatcgtca tgccaggccg ccactgatcc tgcggcttaa
    35221 gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc
    35281 cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg tcggtttttt
    35341 gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc atatacaaca
    35401 agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa
    35461 ccgtcagcac cgaaagcgct gtcaccagca gcgtggtttt cggccagtgc agtactttca
    35521 gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg ctttccggcg
    35581 gaattttgcc acggatccag taccccatca ggatagggat cactacgatc gccagcagcg
    35641 ccgcgcccgc catcgcatag gttttagtga acgccaacgg gccaaacaga cgcccttcct
    35701 gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaata
    35761 gcgccggacc cacttcaaca gatgcatcgg tgatcacctg ccagcgcgtt ttattatcca
    35821 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgtgca ttttcgatca
    35881 tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccgccc aacgacataa
    35941 tattggcatt cagtccctgg aagtgcatga caataaaagc aatacacaac cccagcggca
    36001 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca cagactaccg
    36061 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcg cggtcaatga
    36121 gctggctgcg atcgtatgtt gtaactatct ccacgccttc cggcagacta cttttcagcg
    36181 tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg gatcgcagga
    36241 tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc cggcgcatct
    36301 ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca ttttcactgg
    36361 cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg gcgcgcacca
    36421 tatattccgc ttccgccagt tcgatcgacg aaccgcccgc ttcctggttt gaagcatcca
    36481 gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctgg ggatcgataa
    36541 ccacctgata ctctttcacc acaccgccca ccgacgccac ttccgcaacg tcagggatgg
    36601 ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggcc agatcgtgct
    36661 taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg gcatctggtc
    36721 ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg tactccagca
    36781 cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac acataagagt
    36841 cgccaaactg tgagaaaccg cgcacagtct tcgcgccagg caccgacaac atggtggtgg
    36901 ttagcggata agtcacctga ttttcaacga tttgtggtgc ctgaccggga tagctggttt
    36961 taataataac ctgcacatcg gagagatccg gcagcgcatc cactggcgta ttaatgatgg
    37021 tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa cggttcgcca
    37081 ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat gggtagcact
    37141 ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa tcaggaacag
    37201 gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg tgacgccttg
    37261 cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat caacggtaat
    37321 cacccgctgt tcgctgccgg tatcaatcag cgcttgtgac ggaatgagca gcatcggttc
    37381 gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct cgtcggcgtt
    37441 gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag gtagcagcgt
    37501 ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg taaactgcga
    37561 ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc acaccgggtc
    37621 cataccctga attttcgcga ccacgttatc tttggcgata ttcattcccg cgcgcagatc
    37681 aaacgcggtg atcacgccat caatgggcgc tttgagcgta aagcgagtct ggattttttg
    37741 cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca gccgctcaag
    37801 aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat actcagtctg
    37861 cgcttccacc cagtcaggaa tggtcaggtc gagaagcggt gtgccctttt gtactttatc
    37921 gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct gcacaatggc
    37981 atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag tcagcggtcc
    38041 gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt caatgcgcac
    38101 accagacgca gaactctctt catcggcata tttcggcacc agatccatat ccataaacgg
    38161 cgatttacct ggtttatcga accgcgtatt gggatacatc gggtcgtacc agaataagat
    38221 tttacgttct gcggtcgacg ttttttctgc aggcggttcc gcctttgcaa cccaggtaaa
    38281 acctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg cgattttttt
    38341 catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag aaaggttgcc
    38401 ctgctggaca aaattaaacg ccactttgtc gccagtttta atttcactca ttttcgtctg
    38461 cggagtgatg gtaaagcgca tggtcatctc cggccagttc acggcagcaa tcggatcgtg
    38521 atggatggtg atttttttgc tttccagatc aatacccttt accacgccag tggcgctaat
    38581 aacctgcggt tgtacttcgc tcatggtttc atgatgatgt tcgttagcct gggcattaaa
    38641 gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca tttattctct
    38701 cctggagtta aaagttattg ctgccaaccg ccaccaagtg cggtatataa agaaatttcg
    38761 ttaacctgac gggcataatt caggtcaagt aaagtttgtc gggttgcaaa taacgaacgt
    38821 tcggcatcca gcacttccag ataacttact gcgccgtgct gatataatgc ccgcgcccgt
    38881 tgcaaagtaa tttgcagcga tgccagataa cgctgctggg cgctaatttg atcgttcagg
    38941 ctttgacgta atgccagcgc atctgccact tctttaaagg cgttctggat tttctgttca
    39001 taattcacta ccgactgctg ctggcgaatt tcggcgatat ccagattggc ctggttgcgt
    39061 ccggcattaa aaatggggat ctcaatttta ggaataaaat tccacatccc gctgctggcg
    39121 ttaaataatg acgacagatc gctgctggcg gttgatattc cgctggtcag gcttatagat
    39181 gggaaaaatg ccgcgcgtgc ggccccaata ttggcattag ccgccattaa cgcgtgttca
    39241 gcttccataa tatccgggcg ctgcaataag atttgcgacg acaagcccgc cggtaattta
    39301 acgctttgca ggctgtcgct gtttactgtc tgcgcttgcg gcagcttgcc gtagcttccc
    39361 agcaacagtt gcaatgcatt attcgcctgc gccagttccc cctgacgttt agcgatgtcg
    39421 ctgcgggtac tttctatcac cccgcgagcc tgttccagcg ccagaacatt actgctacct
    39481 gtcaacagtt gtttttcgac aaacgcatat gactgctgat aattacgcag cgtttcttca
    39541 gctatttgca attgcgcata cgccagttgc tgattgaaat agctttgcgc gacattagaa
    39601 accagcagaa tatgcaccgc gcgctgagct tcctcagtag ctaaataatt ttggcgctcg
    39661 gcttcgctca tgttctttaa gcgaccgaaa aaatcgaggt caaagctggc gttaaggccg
    39721 gtcgaaaact cccgtgtcgt ggcggtattc cctttaagat taccgctcca gctgccgctg
    39781 ccctcgccat tgagctgtgg gtagcggtcg gcatcggtca gacgatattg cgcccgtgcc
    39841 tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat tcaccagcgc ctcgctaatc
    39901 agcgtcttca cctgattatc aacaaaaaag gtgcgccagc ccgcgttctg atagttatct
    39961 gctgcgttaa cgaggccgtt ctggctgagt gagaactgct gcggtacggg cattgccggg
    40021 cgctgataat ccggtgccag tgaacaaccg gttagcgcaa gggccacaca aaatggcaga
    40081 agtttacaag gagacatagg ctcataattt ctgatgattt tatgccgcca actttactcg
    40141 ccaggctctg attttccggt gacaggaaaa tgacaaaatt gtcattttgc caataagcga
    40201 ttgccatctg atcccgctac tctagaattg cccgggcaac atgcggagga aatatgaaac
    40261 tgttgattgt cgaagatgaa aagaaaaccg gagaatactt gaccaaaggg ttaaccgaag
    40321 ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg ctaccatctg gcgatgaccg
    40381 gtgattatga tctgataatc ctcgatatta tgctgccgga cgtgaacggc tgggatatcg
    40441 tgcgcatgtt acgctccgcc aataaaggga tgccgattct gttgcttacc gcgcttggca
    40501 ccattgaaca tcgcgtcaag gggctggagt tgggggcaga tgactacctg atgaaaccat
    40561 tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct gcggcgcggg gcggcggtaa
    40621 ttatcgaaag tcagtttcag gttgccgacc tgatggtcga tctcgtcagc cgcaaagtca
    40681 cccgcagcgg cacgcgcatc actttgacca gtaaagagtt tactctgctg gagttttttc
    40741 tccgccatca gggcgaagtg ctgccccgct cgcttatcgc ctcgcaggtg tgggacatga
    40801 atttcgacag cgacactaac gccattgatg tggcggtgaa gcggctacgc ggcaaaatcg
    40861 acaacgactt tgagccgaag ctgattcaga ccgtgcgcgg cgtgggctat atgcttgagg
    40921 tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc tggcaacccg tctgaccttt
    40981 tttatcagcc tggccaccat cgcggcgttt ttcgcctttg catggatcat gatccactca
    41041 gtgaaagtgc attttgccga gcaggatatt aatgatttaa aagaaattag cgccaccctt
    41101 gaacgggtac ttaatcatcc tgacgagacg caagcccgac gcttaatgac gctggaagat
    41161 atcgtcagtg gttattccaa cgtgttgatt tccctggcag atagccacgg caaaacggtg
    41221 tatcactccc ccggtgcgcc ggatatccgc gagtttacgc gtgacgccat acccgataaa
    41281 gacgcgcggg gcggtgaggt gtatctcctt tccggcccga cgatgatgat gccaggccac
    41341 ggtcacgggc atatggaaca cagcaactgg cggatgatta acttgccggt tggcccgttg
    41401 gtggacggca aaccgattta tacgctctac atcgcgcttt cgatcgattt tcatcttcat
    41461 tacataaatg atttgatgaa taaacttatt atgaccgcat ctgtcatcag catcctgatc
    41521 gtctttatcg tgctattggc ggtacataaa ggtcacgcgc cgatccgcag cgtcagccgt
    41581 caaatccaga atatcacctc gaaagatctc gacgttcgcc ttgacccgca gaccgtgccc
    41641 attgagctgg aacagctggt actgtcgttc aaccatatga tcgagcgtat tgaggatgtc
    41701 tttacccgcc agtccaattt ctcagcggat atcgctcacg aaattcgcac gccgattacc
    41761 aatctcatca cgcaaacgga aatcgccctc agccagtctc gcagccagaa ggagctggaa
    41821 gatgtgctct actctaatct cgaagagctg acgcgaatgg cgaaaatggt cagcgatatg
    41881 ctgtttctcg ctcaggccga taacaaccag ctaatccccg aaaagaaaat gctcaacctg
    41941 gcggatgaag tcggcaaagt gttcgatttt ttcgaggcgt tagcggaaga tcgcggcgtg
    42001 gagttgcggt ttgttggcga caaatgtcag gttgcgggcg atccgctgat gctgcgtcgg
    42061 gcgttaagca acctgctttc taatgccctg cgttatacgc cacccagtga ggctattgta
    42121 gtgcgctgcc agacggtcaa tcatcaggtg caagtttccg tcgaaaaccc cggtacgccc
    42181 attgcgcccg agcacttacc gcgattgttt gaccgtttct atcgcgttga cccttcccgc
    42241 cagcgaaaag gtgaaggtag cggtattggg ctggcgatag tgaaatcgat tgttgtcgcg
    42301 cataaaggca cggttgcggt aacgtcagat gcgcggggga caaggtttgt gattatgctg
    42361 ccggagagag agtgattatt aagttcaaat tcagggcgtg gtgcatcacc tcctggtatt
    42421 gttgatcaac aaattagaga aaaacttatt aaacatatga aatatcgaag gctttt
//
LOCUS       DAFAVF010000022        40503 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFAVF010000022 DAFAVF010000000
VERSION     DAFAVF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.022"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..902
                     /locus_tag="LGK42_001174"
     CDS             <1..902
                     /locus_tag="LGK42_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBK2657448.1"
                     /translation="LYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPV
                     TGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGT
                     SCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKA
                     MGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVD
                     KGKIYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVER
                     LKMLADERLYKSKKNGRAQISWQ"
     gene            complement(949..2274)
                     /gene="rimO"
                     /locus_tag="LGK42_001175"
     CDS             complement(949..2274)
                     /gene="rimO"
                     /locus_tag="LGK42_001175"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBK2657449.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            2487..2870
                     /gene="bssR"
                     /locus_tag="LGK42_001176"
     CDS             2487..2870
                     /gene="bssR"
                     /locus_tag="LGK42_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBK2657450.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2981..4096
                     /gene="yliI"
                     /locus_tag="LGK42_001177"
     CDS             2981..4096
                     /gene="yliI"
                     /locus_tag="LGK42_001177"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBK2657451.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(4093..4719)
                     /gene="gstB"
                     /locus_tag="LGK42_001178"
     CDS             complement(4093..4719)
                     /gene="gstB"
                     /locus_tag="LGK42_001178"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBK2657452.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            4965..6167
                     /gene="dacC"
                     /locus_tag="LGK42_001179"
     CDS             4965..6167
                     /gene="dacC"
                     /locus_tag="LGK42_001179"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBK2657453.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(6214..6972)
                     /gene="deoR"
                     /locus_tag="LGK42_001180"
     CDS             complement(6214..6972)
                     /gene="deoR"
                     /locus_tag="LGK42_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBK2657454.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(7030..7626)
                     /gene="ybjG"
                     /locus_tag="LGK42_001181"
     CDS             complement(7030..7626)
                     /gene="ybjG"
                     /locus_tag="LGK42_001181"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK2657455.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7911..9143
                     /gene="mdfA"
                     /locus_tag="LGK42_001182"
     CDS             7911..9143
                     /gene="mdfA"
                     /locus_tag="LGK42_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBK2657456.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(9184..9468)
                     /locus_tag="LGK42_001183"
     CDS             complement(9184..9468)
                     /locus_tag="LGK42_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657457.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(9554..10369)
                     /gene="ybjI"
                     /locus_tag="LGK42_001184"
     CDS             complement(9554..10369)
                     /gene="ybjI"
                     /locus_tag="LGK42_001184"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBK2657458.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(10369..11577)
                     /locus_tag="LGK42_001185"
     CDS             complement(10369..11577)
                     /locus_tag="LGK42_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657459.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            11661..12197
                     /gene="rcdA"
                     /locus_tag="LGK42_001186"
     CDS             11661..12197
                     /gene="rcdA"
                     /locus_tag="LGK42_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBK2657460.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(12372..14057)
                     /locus_tag="LGK42_001187"
     CDS             complement(12372..14057)
                     /locus_tag="LGK42_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBK2657461.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHFGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            14327..14704
                     /locus_tag="LGK42_001188"
     CDS             14327..14704
                     /locus_tag="LGK42_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBK2657462.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(14734..14991)
                     /gene="grxA"
                     /locus_tag="LGK42_001189"
     CDS             complement(14734..14991)
                     /gene="grxA"
                     /locus_tag="LGK42_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBK2657463.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            15151..15438
                     /locus_tag="LGK42_001190"
     CDS             15151..15438
                     /locus_tag="LGK42_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBK2657464.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            15422..16144
                     /gene="nfsA"
                     /locus_tag="LGK42_001191"
     CDS             15422..16144
                     /gene="nfsA"
                     /locus_tag="LGK42_001191"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBK2657465.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            16205..17107
                     /gene="rimK"
                     /locus_tag="LGK42_001192"
     CDS             16205..17107
                     /gene="rimK"
                     /locus_tag="LGK42_001192"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBK2657466.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            17195..17671
                     /locus_tag="LGK42_001193"
     CDS             17195..17671
                     /locus_tag="LGK42_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBK2657467.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            18023..19135
                     /gene="potF"
                     /locus_tag="LGK42_001194"
     CDS             18023..19135
                     /gene="potF"
                     /locus_tag="LGK42_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBK2657468.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            19230..20363
                     /gene="potG"
                     /locus_tag="LGK42_001195"
     CDS             19230..20363
                     /gene="potG"
                     /locus_tag="LGK42_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBK2657469.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIVSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            20373..21326
                     /gene="potH"
                     /locus_tag="LGK42_001196"
     CDS             20373..21326
                     /gene="potH"
                     /locus_tag="LGK42_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBK2657470.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            21323..22168
                     /gene="potI"
                     /locus_tag="LGK42_001197"
     CDS             21323..22168
                     /gene="potI"
                     /locus_tag="LGK42_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBK2657471.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            22228..22716
                     /locus_tag="LGK42_001198"
     CDS             22228..22716
                     /locus_tag="LGK42_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBK2657472.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            22757..23884
                     /gene="rlmC"
                     /locus_tag="LGK42_001199"
     CDS             22757..23884
                     /gene="rlmC"
                     /locus_tag="LGK42_001199"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBK2657473.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(24059..24790)
                     /gene="artJ"
                     /locus_tag="LGK42_001200"
     CDS             complement(24059..24790)
                     /gene="artJ"
                     /locus_tag="LGK42_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBK2657474.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(25082..25750)
                     /gene="artM"
                     /locus_tag="LGK42_001201"
     CDS             complement(25082..25750)
                     /gene="artM"
                     /locus_tag="LGK42_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBK2657475.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(25750..26466)
                     /gene="artQ"
                     /locus_tag="LGK42_001202"
     CDS             complement(25750..26466)
                     /gene="artQ"
                     /locus_tag="LGK42_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBK2657476.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(26473..27204)
                     /gene="artJ"
                     /locus_tag="LGK42_001203"
     CDS             complement(26473..27204)
                     /gene="artJ"
                     /locus_tag="LGK42_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2657477.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(27222..27950)
                     /gene="artP"
                     /locus_tag="LGK42_001204"
     CDS             complement(27222..27950)
                     /gene="artP"
                     /locus_tag="LGK42_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBK2657478.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(28168..28683)
                     /locus_tag="LGK42_001205"
     CDS             complement(28168..28683)
                     /locus_tag="LGK42_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2657479.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            28809..29132
                     /locus_tag="LGK42_001206"
     CDS             28809..29132
                     /locus_tag="LGK42_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK2657480.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            29129..29959
                     /gene="amiD"
                     /locus_tag="LGK42_001207"
     CDS             29129..29959
                     /gene="amiD"
                     /locus_tag="LGK42_001207"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBK2657481.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(29956..30969)
                     /locus_tag="LGK42_001208"
     CDS             complement(29956..30969)
                     /locus_tag="LGK42_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK2657482.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(31068..32498)
                     /locus_tag="LGK42_001209"
     CDS             complement(31068..32498)
                     /locus_tag="LGK42_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBK2657483.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(32509..33510)
                     /gene="ltaE"
                     /locus_tag="LGK42_001210"
     CDS             complement(32509..33510)
                     /gene="ltaE"
                     /locus_tag="LGK42_001210"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBK2657484.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(33547..35265)
                     /gene="poxB"
                     /locus_tag="LGK42_001211"
     CDS             complement(33547..35265)
                     /gene="poxB"
                     /locus_tag="LGK42_001211"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBK2657485.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPIVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(35398..36366)
                     /gene="hcr"
                     /locus_tag="LGK42_001212"
     CDS             complement(35398..36366)
                     /gene="hcr"
                     /locus_tag="LGK42_001212"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBK2657486.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(36378..38030)
                     /gene="hcp"
                     /locus_tag="LGK42_001213"
                     /gene_synonym="priS"
     CDS             complement(36378..38030)
                     /gene="hcp"
                     /locus_tag="LGK42_001213"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBK2657487.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(38174..39073)
                     /gene="lysO"
                     /locus_tag="LGK42_001214"
     CDS             complement(38174..39073)
                     /gene="lysO"
                     /locus_tag="LGK42_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBK2657488.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(39568..40263)
                     /gene="aqpZ"
                     /locus_tag="LGK42_001215"
     CDS             complement(39568..40263)
                     /gene="aqpZ"
                     /locus_tag="LGK42_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBK2657489.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9927 a        10253 c        10561 g         9762 t
ORIGIN      
        1 acctctatcc ggtaactcac aatcgtagtc tgatattttt gcttggtctg gagcgttttt
       61 ctttactgtc aaaatcgctg gcgatggaca gcgagaacct gatgttctct ctatttaaga
      121 acggtaaacc ggtgaccggt gatgaatata atgctaaaaa cgccatcttc accgtttcgg
      181 aagcgatgga gcacttcgcc tatttgccga ccggattgta cgtatttgcg tataaaaaag
      241 atgtttattt gcgggtctgt acattgatta ttttctttgc cgcattggtg gcagtgatat
      301 cgggtaccag ttgcctctat ctggtacgca gagtgattaa tcgtggtatt gtggagaaag
      361 aagccatcat taataaccat tttgaacgcg tactggatgg cgggcttttc ttttcggctg
      421 ccgatgtcaa aaaactctac agtatgtata actcggcgtt cctggacgac ctgaccaaag
      481 caatgggcag aaaatccttt gacgaagatt taaaagcgct gccggaaaaa ggcggttatt
      541 tgtgcctgtt tgacgtcgat aaattcaaaa acattaacga caccttcggt catttgctgg
      601 gcgatgaagt gttgatgaaa gtggtgaaaa tccttaaatc acagatcccg gtagataaag
      661 gtaaaatcta ccgcttcggc ggtgacgaat ttgcggtgat ttatacgggt ggaacgctgg
      721 aagagttgct atcgattcta aaagaaatcg ttcatttcca ggtgggaagc attaatttaa
      781 gtaccagtat cggtgtagcg cattcaaatg aatgtaccac cgtcgaacgc ttgaaaatgc
      841 tggcggatga gcggctgtat aagagtaaga aaaacggcag ggctcagata agctggcagt
      901 aatcattatt cgcaggccgg acaaatgatt ttgcccggcc tgaattaatt aaacccggct
      961 accccacaaa tcgtactcat cggcgtgctc gactttcaca cgcaggatat cacccggctt
     1021 aacgttggtt tcaccattga gataaaccgc gccgtcgatt tccggtgcat ctgccatgct
     1081 gcgaccaatc gcgccttctt cgtccacttc gtcgataatc accagaattt cacggcccac
     1141 tttctcttgc aggcgctcgg cggaaatctg ctgctgcagc tgcatgaaac ggttccagcg
     1201 ttcttcttta acttcttccg gaacctggtc aggcagggca ttggcgtctg caccttcaac
     1261 cgggctgtat ttaaagcagc caacgcgatc cagacgcgct tctttcagga agtcgagtag
     1321 catctggaaa tcttcttctg tctcgccagg gaagccgaca ataaaggttg agcgtagggt
     1381 cagttccggg cagatttcgc gccactgttt gatgcgcgcc agttggcgat ctacagaacc
     1441 cggacgcttc atcagtttga gaatgcgcgg gctggcgtgc tgcaacggaa tgtccagata
     1501 cggcaggatt ttgccttctg ccatcagtgg gatgacgtcg tccacatgcg gataagggta
     1561 aacgtagtgc agacgtgtcc agatccccag tttcgataac tgttcgcaca ggctgaccat
     1621 gctggttttt accggctcgc cgttgtggaa gccagtacga tgtttaacat caacgccata
     1681 ggcggaagta tcctgcgaga tcaccagaat ctctttaacg cccgcatcta ccagacgttt
     1741 cgcttcactt aacacttcgc caatcggacg gctcaccagg tcgccgcgca tagacggaat
     1801 aatgcagaag gtgcagcggt gattacagcc ttccgaaatt ttcagatagg cataatgacg
     1861 cggcgtcagt ttcacacctt gttctggcac caggctcagg aatgggttgt gtttcggttt
     1921 tggcacgtag tgatgaacgt gctccagaac ctgctcatag ctatgaggcc cggtgatttc
     1981 cagcactttc gggtggactt cgcggatctg atcttctttt gcccccagac aaccggtcac
     2041 aattaccttg ccgttttcat tcaacgcttc accaatggct tccagtgatt cttgtaccgc
     2101 gctgtcaata aagccgcagg tgttgacgat caccatgtcc gcatcgtcat agctcggtac
     2161 cacgtcataa ccttcagtgc ggagttcggt gagaatacgc tctgaatcaa caaggttttt
     2221 tggacagcca agggaaacaa agccgatttt cggctgggga gttactttgc tcatagctta
     2281 aaaaatattc agttacagga aaggtcaggg cagggattct acagagttct gcataaaatt
     2341 tgtatcgcaa tctcattcgc tggcggaggc gaaggaaatg taaattttgt taattcggcg
     2401 tgaagaattg atcctggaca gcattttgct caaaaaatag ccatactatt taattgcaac
     2461 aaggctggaa agaggaggat cgaagtatgt tcgttgacag acagcgaatc gatctgctga
     2521 accggttgat cgacgcacgc gttgacctcg ccgcatacgt gcaactgagg aaggcaaaag
     2581 gatacatgtc cgtcagcgaa agcaatcatc tacgagataa cttttttaaa ctgaatcgcg
     2641 aactgcacga taaatcgctg cggttgaatc ttcatctgga tcaggaagag tggagtgctc
     2701 ttcatcatgc tgaagaagca ttagcgacag ccgcagtatg tttgatgagt gggcaccatg
     2761 attgcccgac tgttattacc gtcaacgccg ataagcttga aaattgtctg atgagcttaa
     2821 cgctgagtat ccagagcctg cagaagcacg ccatgcttga gaaggcctga aaactaaggg
     2881 ggagaaagcg tctccccctt catgtttaag tttttgtaaa aatgaatttg ttatctcctc
     2941 cactgactac gctttaagcc agagtcaatc cggaggcgtt atgcatcgac aatccttttt
     3001 ccttgtgccc cttatttgtc tttcttccgc tctctgggcg gctcctgcaa cggtaaatgt
     3061 cgaagtactg caagacaaac tcgaccatcc ctgggcactg gcctttttac ccgataatca
     3121 cggtatgtta atcactctgc gcggcggcga gttgcgtcac tggcaagcag gaaaaggatt
     3181 atctgcgccg ctttccggag ttccggacgt ttgggcgcac gggcagggcg gcctgctgga
     3241 cgtggtttta gcgcctgatt ttgctcagtc tcgccgcatc tggttaagtt attccgaagt
     3301 tggcgatgat ggcaaagccg gaactgctgt gggttatggc cgcttaagtg atgatctctc
     3361 aaaagtgacc gacttccgca ccgtcttccg ccagatgcca aaactgtcta ccggcaacca
     3421 ttttggcggg cggctggtat tcgacggtaa aggttatctt tttattgctc tgggcgaaaa
     3481 caatcagcgc ccgacggcgc aggatctgga taaattacag ggcaaactgg tgcgtctgac
     3541 cgaccagggc gaaatcccgg atgataatcc ttttataaag gaatccggtg tgcgcgccga
     3601 gatctggtct tatggcattc gtaatccgca aggaatggcg atgaatccgt ggagtaatgc
     3661 actgtggctg aatgaacatg gcccgcgcgg tggtgatgaa attaatatcc cgcaaaaagg
     3721 caaaaactac ggctggccgc tggcaacctg gggaatcaac tattcaggct ttaagatacc
     3781 ggaagcgaaa ggggagatcg tcgccgggac cgagcaacct gttttttact ggaaagattc
     3841 gcccgctgtg agcggcatgg ccttctataa cagcgataaa ttcccccagt ggcagcaaaa
     3901 attatttatt ggcgcgctga aagataaaga tgtcattgtg atgagcgtca acggcgacaa
     3961 agtgacagaa gatggccgta ttttaacgga cagagggcag cgaattcgtg atgttcgcac
     4021 tggacccgac ggttatttat acgttctcac cgacgagtcc agtggggaat tacttaaagt
     4081 tagcccacgc aattagctaa cgggaatcat caccactttg cgcactgcgg ggcgttcagt
     4141 gagttgctga taccagcgtt gcagatttgg acgcggtgtc caggtcaggc caacgttaaa
     4201 caaattgtag atgaacggtg cgatagcgat atcgcccaca ccaaactcgt cgccagagaa
     4261 ccattttact tttgccagtt ccgcatcgag cagggcaaac agggcgtcgc actctttgca
     4321 actggcatca atggcggcct gatcgcgctc ttccggtggt gttctgacta atcccatcag
     4381 gatcccgcga tgagcattac tgagcgtctg gtttgcccag tccatccatt tttccgcttc
     4441 cgcacgacgt gccggtgagt cgatccacag acgtttttgc ccgtactgtg ccgccagata
     4501 gcggacaatg gcgtttgatt cccaaagaat gagatcactt tcgtcgtcac gcaacaacgg
     4561 caccagcccg ttagggttca tcgccagaaa atcagcatcg tgatttatcc caaactcacg
     4621 gcccgcgaga atttgctcat aaggtagttc cagttcttcg agcgtcagca atactttttt
     4681 tacgttagtt gaattattcc gaccccacag cgtaatcata ctcacccctt ttccgacagg
     4741 cagccacagg ctgaacagga ctccgatggt gaagtaaagt taacttttaa gcaacagctg
     4801 gcaaaaaaat gacaccagaa gcagcgaagc ggcaggatat tgcataaact ttaaaaactt
     4861 taccaactta cggtttcttt aagtttgtgt gtgcgttatt aatcaccgaa cttatcatac
     4921 ggcgatataa cgtatttttt tgaatggata ctcgggtggc atttatgacg caatactcct
     4981 ctctccttcg tggtcttgca gcgggttctg catttttatt cctttttgcc ccaacggcat
     5041 tcgcggcgga acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg attttaatgg
     5101 attacgccag cggtaaagtg ctggcagaag gcaacgcgga tgagaaactg gatcccgcga
     5161 gcctgactaa aatcatgacc agctatgtgg ttgggcaggc gcttaaggcc gataagatta
     5221 aactcaccga tatggtgacg gtcggtaaag atgcctgggc gacgggaaat ccggcactgc
     5281 gtggttcatc ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca gacttgaaca
     5341 aaggtgtgat tatccagtcc ggtaatgacg cctgtattgc gctggccgat tacgttgccg
     5401 ggagccagga gtcatttatt ggtttgatga atggttatgc caaaaaactg ggtctgacca
     5461 acactacctt ccagacggtg cacggtctgg atgcgccggg gcagttcagt accgcgcgcg
     5521 atatggcatt gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac gccattcata
     5581 aagagaaaga gttcaccttc aacaaaattc gtcagcctaa ccgtaaccgt ctgctgtgga
     5641 gcagcaatct gaatgttgat ggcatgaaga caggaaccac ggcaggcgcg ggatataatc
     5701 tggttgcttc ggctacccag ggcgatatgc gtttaatctc cgtagtgctg ggggcgaaaa
     5761 ctgaccgtat ccgttttaat gagtctgaga aattattgac ctggggtttc cgcttctttg
     5821 aaaccgtgac gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc tggtttggtg
     5881 ataagagcga agtgaatctt ggggcaggtg aagcgggctc agtgaccata ccgcgtgggc
     5941 agctgaaaaa cctgaaggcg agctacacgt taacggaacc gcagcttacc gcaccgctga
     6001 aaaaaggtca ggttgtcggg accattgatt tccagcttaa cggtaaatcc attgagcagc
     6061 gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt
     6121 tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc
     6181 ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa tacggtgcgt
     6241 ctgcgcgtac ttcacatact catcttccgg gcaacaatca ctcaccacaa tatcaaagcg
     6301 tttcaggtca cccatgcgcg ccggacgcac cttgccaaat ttactgtggt cgacaaccag
     6361 cacatgcttt tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa
     6421 acaggtagcg cctttactga catgcacgcc cgccgcggaa taaaaagcga tatccgggca
     6481 aaaattattc aacgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa
     6541 ttcaccaccg cacagaaatg cgcggcaatg gggtttctct ttcagcgcca gaaaggtatt
     6601 tagcgaataa caaacggcgg taaaagggat ttcattatca atcgcttcaa taatccacgg
     6661 cgtggtggtg ccacagtcaa aaaagagagt ctgatcgggt tctaccagtg tcgcagccat
     6721 ttttgccgcc cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg
     6781 gctggcactg cgcggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg
     6841 gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt
     6901 aagatgtaac ttatcgctgc gttttaattc ttgcagcagc tgcccgatac gctcttcgcg
     6961 acgtgtttcc ataatccctc tgaatagtta ttgaagcgag ccgctcaata ctacactttt
     7021 tagcagagat cagtcacgca cccagccttt gcggatcggt aatgcaaaac agaagcgata
     7081 ccacgattgc agacgttgat agagtttatg ccccatcgct tgccagataa tctgagcgct
     7141 aaggcagcca atcatacctg ccagcaatcc accgagcata tccagcggcc agtgcacgcc
     7201 aagataaacg cgcgaccagg caatgacgac ggccagcacc attaaaagtg agccggacca
     7261 caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac cgtggtcgct
     7321 tgggaatgag tcatccgccg catgatgcag gaagttatag ccgatatttt cgacaaaggg
     7381 tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca aacaggctga ccgccagcgc
     7441 gatagcgatt tttatcacca gttgccgttg tgctgtaagc ccccacaacc aaagcaccgc
     7501 ggccagcagc ggtaccacgg taatcaaatc tttagcaata aaaatcgcca acgagatcat
     7561 ccacggagcc gagtctggcg tcgcgttaat aagagagaat agagagagat tcaaattttc
     7621 cagcataact tcccgacgca aagtgattaa aagggagcca atacaggcaa gtcgttgaga
     7681 ataaagtgca ggttaaactg ggtaaagcgg catcgtctta tttccctcaa gcggcctgtt
     7741 tacggtgggt aattgtaacg ggcataggtt aaataaaact taaagaaagc gtagctatac
     7801 tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg ctttctcgtt
     7861 agctgtgctt ttattaaact ctgcgcgatt attattggcg aagaaattgc atgcaaaata
     7921 aattagcttc cggtgccagg cttggacgtc aggcgttact tttccctctc tgtctggtgc
     7981 tttacgaatt ttcaacctat atcggcaacg atatgattca acccggtatg ttggccgtgg
     8041 tggaacaata tcaggcgggc attgattggg ttcctacttc aatgaccgca tatctggcgg
     8101 gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga tcgtattggt cgccgtccgg
     8161 tgatgctggc gggggtggtg tggtttatca tcacctgtct ggcaatatta ctggcgcaaa
     8221 acattgaaca attcactctg ttgcgcttct tgcagggcat aagcctctgt ttcattggcg
     8281 ctgtgggata cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc aagatcaccg
     8341 cgctgatggc gaacgtggcg ctgattgctc cgctacttgg tccgctggtg ggcgcggcgt
     8401 ggatccatgt gctgccctgg gaagggatgt ttgtcttgtt tgccgcattg gcagcgatct
     8461 cctttttcgg tctgcaacaa gccatgcctg aaaccgccac gcgtataggc gagaaactgt
     8521 cactgaaaga actcggtcgt gactataagc tggtgctgaa gaacggccgc tttgtggcgg
     8581 gggcgctggc gctgggattc gttagcctgc cattgctggc gtggatcgcc cagtcgccga
     8641 ttatcatcat taccggcgag cagttgagca gctatgaata tggcttgctg caagtgccta
     8701 ttttcggggc gttaattgcg ggtaacttgc tgttagcgcg tctgacctcg cgccgcaccg
     8761 tacgttcgct gattattatg ggcggctggc cgattattat tggtctgttg gtcgctgctg
     8821 cggcaacggt tatctcatcg cacgcgtatt tatggatgac cgccgggtta agtatttatg
     8881 ctttcggcat tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata
     8941 tgagtaaagg tacggtttct gcggcgatgg gaatgctgca aatgctgatc tttaccgtcg
     9001 gtattgaaat cagcaaacat gcctggctga acgggggcaa cggactgttt aatctcttca
     9061 accttgtcaa cggaattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga
     9121 tgggaaattc tcacgaaggg taaaaaaatg cctgattgct ttgtgcgatc aggcattctc
     9181 gaattaatgg tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc
     9241 aagatggcga acataaggat gcgggcggta agccggagct ggcgcaggag ccacatagac
     9301 ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac
     9361 aaccactttg tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt
     9421 aaagcccatt aaaagcgcgc ccaacagtag acaatttttc ataaacattt cgcctgaaat
     9481 aatgcagcgg gttaatttcc gcgaattatg cagccttcat tcaggtattg tgttgccggg
     9541 cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt aagcacttta tcgatcacat
     9601 ccagtacgcc ttcacggtta ttggagcctg cccggtattt tgccgctgcg acgaccgcat
     9661 tgccggcatt ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac
     9721 cgccatcgcc aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc
     9781 gaaggccatt ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt
     9841 ggaccggcac cataatatcg ccgatggcct catgtaatgc tttttgtact tgtggaatca
     9901 gttcatcgga aagattcagg ccaaacttaa agaagatatc ctctaagttg tcaaagttat
     9961 cgacgtattc cagacggtga taatacattt ccgccaccgt tttcatggca tcgtcatatt
    10021 ttttgagcgt ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca
    10081 gcaaatgttc cacgacagta gtaaacgcat ccttcgacag ctcgccatta aaaacatctt
    10141 tgccttcgct cactacccag ccgccgtttt cagccacaaa ggcaatttca ttagcaattt
    10201 cagggaagaa agagatcaac tgataatatt gattcccgct ggcgactaca aagcgaattc
    10261 cttgtgcttt catttgctga tactgagcca taaaccgctc acggttatag gttttttgat
    10321 cgcttaagaa agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc
    10381 catcgccgtc tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag
    10441 aataaccagc gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata
    10501 gcccagcagc ggcggcccga cgaggaaagc cagataaccg gtcgttgcta ccacactgac
    10561 gcgggttggt gcatcggggc cggtatcgct ggcggcagaa atggtcagcg ggaagcccag
    10621 cgaggcaccc agtccccaga gtacaacaga caccccagcg acccaggcgc tatcgacaaa
    10681 aataatcagc ccaataccca acgcccccat tagtgcactg gcccgaacca cggcaacgcg
    10741 actgtaacgg tcgatgaacc aaccgccggt gaagcgtcca acggtcatcc ccagggtaaa
    10801 accggcataa atcagcgagc cggaagtagg gctaaaaccg tgaccgtcaa ccattaataa
    10861 gggtaaccag tcgttggcag aaccttcggc aaaggccatc gccagcacca caacacctat
    10921 cagcagcaac tggatatcgc gataaaaagg aatgcctttt tcgccatgct gggtgccatc
    10981 ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg gcgatataaa taggcgcgat
    11041 acctaccagc gccgccaata aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc
    11101 catcccgaca ccagcgcctg ccagcgtgcc caggctataa aaaccgtgca tcattggcaa
    11161 aaccgtttta ttcatttctc gctcaacggc ggcaccttca acgtttatcg ccacttccgc
    11221 agaaccaaaa cttgccccaa agacgccgag accaacggca aagagcaggg gcgatgtcag
    11281 ccagagtgcc agacttaata tcatcatccc gatcaatgcg caggacatcg tgaccaggat
    11341 gacattacgc gtgccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga
    11401 accgatcgac agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc
    11461 gcggatagca ggcgtacggg ttgcccagga cgccattaac aggcctggca aaaagaagaa
    11521 cataaacagc gcccaggttc ggcgtttcaa tgcattacgt gaagaattta cggtcataga
    11581 tcacgtcaaa ataagaagag ggaagacaac attagcaagg ttgtgtacat ttgtacacaa
    11641 ttgcagagag aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta
    11701 tccaggccac gctggaggcg gtgaaacttt acggaataca tgctgttacg caccgcaaaa
    11761 ttgctaccct tgccggggta ccgttgggat cgatgaccta ctatttttca ggaattgatg
    11821 agttgttgct ggaggcgttc agcagtttta ctgagattat gtcccggcaa tatcaggcat
    11881 tttttagcga tgttagtgat gctcaaggcg catgccaggc tatcaccgat atgatctaca
    11941 gctcacaggt tgcaacgccg gataatatgg agctgatgta ccagctctac gcgctggcta
    12001 gccgaaaacc gctattaaaa acggtaatgc aaaactggat gcagcgcagt cagcaaacgc
    12061 tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga
    12121 cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga ggagattttg aggatggttg
    12181 agagggttgc agggtagtag ataaatttta gataacaaaa aacccatcaa ccttgaaccg
    12241 aaatggcggg gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa
    12301 ttaagactga tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg
    12361 caacttcagt tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc
    12421 agcaggacgt tggcgattgc gtaggtgccc gcatagccca gcgcagggat gttactgcga
    12481 gctgtatcac tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg
    12541 aacaacagtg cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccacg
    12601 ggcacaagac tgacaattaa tccggcaatc aacatctgac cgccaatcgc gcccaggccg
    12661 ttattaatac cgctaccggc gctcagacca acgcctgcca taaacaccat caagccgaac
    12721 tctttcacca tgcttaatgc accttgcgga atgtaaccga aggtcgggtg gttagcacgc
    12781 ataaagccca gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg
    12841 aatgtgctga actggaaggt gatcatcccg atcatcagcc caataacaaa gaaggcgcaa
    12901 aatgccagca ggtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg
    12961 gtttttacgc gacgggcatc accgctgact tgtaaaacgt cacctttgtt aagcacgacg
    13021 ttgtcatcta tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga
    13081 tcggtcaact tcagttgtgc gagacgttta cctacagcgt tatggttttt aacgaccact
    13141 tcttcagtga cgatacgcat gtcgagaagg tcacgatcga aaacttcttt accgttacgg
    13201 aagctgggat cgagtcgggc atgggcgtcg ggatagccta ccaacgctat ttcatcgccc
    13261 atttgtagca cggcatcacc gtctggattt gccagaatcc cgttacgtcg aatacgttca
    13321 atgtagcagc cggtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag
    13381 gccaccagct ccgggccgac gcgataggcg cggatcaccg gtaaataaac cttacggttg
    13441 gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga
    13501 tgctgcaatt tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag
    13561 gttaaggcat acccgaggct cagattatcc agtgccagtg agagctgcct gctttccatg
    13621 ccgaaatgac gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcct
    13681 gctaacatac cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg
    13741 atcaccagcg cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga
    13801 aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata
    13861 aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat
    13921 acgactaaaa cgccaatgga attacccagt tggatcgaac caagtcgtaa ctttccgaga
    13981 catagcccaa gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg
    14041 gcgacgttta tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg
    14101 gtaatttacg ataatgtttt ttaccagaat tcagggcgca gattcattca gcgcacctaa
    14161 acgatagtaa agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata
    14221 tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca
    14281 cactggcgaa aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca
    14341 acgttgggcg ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc tttttctggc
    14401 gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct gaaatcggct tgctattttt
    14461 cattcttcct ggagcagttg ccagtttctt ttcacagcgt agagaagtcc tgaaacctct
    14521 atttggcgca atgctggcgg caccctgttc aatgctcatt atgcggctgt ttttttcacc
    14581 gacgcgctca ttctggcaag agctggcatg gttactaagc gcggtgttct ggtgtgcgct
    14641 gggggcactg tgtttcttat ttatcagtag tttgtttaaa ccacagcaca gaaaaaatca
    14701 gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg tccagatttt ctttcaccca
    14761 tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt
    14821 ttctacgggt ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat
    14881 atctacatac tgatactgaa aatcatcgcg ttcattgctc aatttctcag ccagatcttt
    14941 tgcacgcaca cagtaagggc aacccgaacg accaaaaata acggtttgca ttatttctct
    15001 cctcatagat ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa
    15061 cctgttgcat taattgctaa aagctataac tgttaaacac aatacagtga aaagttttag
    15121 actgaaggct cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaggc
    15181 gtgttgatac tggaatttat cggaatgatg ctactggcgg tggcgctgct gtcggtaagc
    15241 gactccctgt cgctgcctga gccattttct cggccagaag tgcagattct gatgattttt
    15301 ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt
    15361 cttgccccac agctgatgaa ccgtccaccg caatattcac gttcagaaag agaaaaagat
    15421 aatgacgcca accattgaac ttatttgtgg ccatcgctcc attcgccatt tcactgatga
    15481 acccatttcc gaagcgcagc gtgaggcgat tattaacagc gcccgtgcga cgtccagttc
    15541 cagttttttg cagtgcagta gcattattcg cattaccgac aaagcgttac gtgaagaact
    15601 ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg gcggagttct gggtgttctg
    15661 tgccgacttt aaccgccatt tacagatctg tccggatgct cagctcggcc tggcggaaca
    15721 actgttgctc ggtgtcgttg atacggcaat gatggcgcag aatgcattaa tcgcagcgga
    15781 atcgctggga ttgggcgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac
    15841 gaaactgctt aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc
    15901 tgcggataat ccggatctta agccgcgttt accggcctcc attttggtgc atgaaaacag
    15961 ctatcaaccg ctggataaag gcgcactggc gcagtatgac gagcaactgg cggaatatta
    16021 cctcacccgt ggcagcaata atcgccggga tacctggagc gatcatatcc gccgaacaat
    16081 cattaaagaa agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg
    16141 ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg
    16201 cagggtgaaa attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg
    16261 tgaagccgct atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat
    16321 gaacataaat cctgcggcgt cttctattca ctacaaaggc cgcaagttac cccattttga
    16381 cgcagtgatc ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca
    16441 gttcgagatg ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga
    16501 caaattgcgt tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat
    16561 tgcgcattcg ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt
    16621 ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg ctggcggaga cgcgtcaggc
    16681 ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata
    16741 tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt gttggcgatg aagtggtcgc
    16801 tgcgattgaa cggcgggcga aagagggcga ttttcgttcc aatttgcatc gtggcggcgc
    16861 ggcaagcgtc gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac
    16921 gatggcgctg gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat
    16981 ggaggtgaat gcgtcgccgg ggctggaagg aatagaaaaa accaccggta tcgacatcgc
    17041 gggtaaaatg atccgctgga tcgaacgcca cgctacgaca gaatattgcc tgaaaacggg
    17101 tggttagtcg caatcacatt actgatcatg gttttgcctg cgctttttgc gtaagctgtg
    17161 ccggtctttt tatcgaaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct
    17221 ggatacgctg cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg
    17281 tcaggctctg catctgcccc atatgcagaa tttcgacggt gtctttgatg ccaaaatcga
    17341 tctgatcgat aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt
    17401 gccgctggcg gcggatttat ctgccatcaa tgccagttcg ctgaccgtga aagcatttct
    17461 tgatatgcag gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca
    17521 gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat
    17581 ggtcattctt gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa
    17641 caacgttacc gaaaactatt ttctccactg ataattcctt tcgagcacgc agtcgctgcc
    17701 gcagtgactg cgcgctgcaa aattatctgc tgtttttaac cttttcttaa agattatttc
    17761 acttatcttg tgtcgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa
    17821 ataccgtttt ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc
    17881 ttttatatag cgagcagtgc tggccgggag agagttctct tttcttacac cgcgccgata
    17941 aaaaaatatg cacgtttatt gcatatcttt cagtgtgaca acttttgttc gtttgttaac
    18001 gaactttcag aaggaaagag atatgaccgc cttaaataaa aaatggctat cgggtctggt
    18061 tgcgggtgct ctgatggccg tctctgtcgg cacgctcgcg gctgaacaaa aaacactcca
    18121 catttataac tggtctgatt atatcgcccc cgacacggtg gctaactttg aaaaagaaac
    18181 cggtattaaa gtcgtctacg atgttttcga ctctaacgaa gtactggaag gcaaattaat
    18241 ggcgggtagc accggctttg acctggtggt gccgtcagca agctttcttg aacgccagtt
    18301 gactgcggga gttttccagc cgctggacaa aagcaaattg ccggagtgga agaatctcga
    18361 cccggagctg ctgaagctgg tcgccaaaca cgatcccgac aataaatttg ctatgcccta
    18421 tatgtgggcg acgaccggga ttggctataa cgtcgataaa gtcaaagcgg tgctgggcga
    18481 aaacgcgccc gtcgatagct gggacttgat cctcaagcct gaaaatctgg aaaaactgaa
    18541 aagctgtggc gtctctttcc tggatgcgcc tgaagaagtt tttgctaccg tgctgaatta
    18601 tctcggcaaa gatcccaaca gcactaaagc agatgattac accggaccgg caaccgatct
    18661 gctgttaaag ctgcgcccga acattcgtta tttccattca tcgcaataca ttaacgacct
    18721 ggcaaacggc gatatttgcg tcgctatcgg ctgggcaggc gatgtctggc aggcgtcaaa
    18781 ccgcgcgaag gaagcgaaga atggcgtgaa tgtctctttc tcgattccga aagaaggggc
    18841 gatggcgttc tttgatgtct tcgccatgcc tgcggatgcc aaaaacaaag acgaagccta
    18901 tcagttcctg aattacctgc tgcgcccgga tgtagtagcg catatttccg accatgtgtt
    18961 ctatgccaac gccaataaag cagccacgcc gctggtgagt gcggaagtcc gtgagaaccc
    19021 gggtatttat ccgcctgcgg atgttcgtgc gaagctgttc actctgaaag tgcaggaccc
    19081 gaaaatcgac cgtgtgcgca cccgcgcgtg gaccaaagtg aagagcggaa aataatccgc
    19141 agccttagat gccgggcggg cgcaccacac ccgccggcaa ttcgcaccat catggtgcgc
    19201 ttgcacacat tcaatgccgg agagctgccg tgaatgacgc tatccctcgc ccccaggcga
    19261 aaacccgtaa ggcgctgacg ccgctattag aaatccgcaa cctgaccaaa tcctacgatg
    19321 gccaacatgc ggtggatgat gtcagcctga ccatctacaa aggtgaaatc ttcgcgctac
    19381 tgggcgcatc cggctgtggc aagtccacgc tgctgcgtat gctggcaggt tttgaacaac
    19441 cttctgccgg acagataatg cttgatggcg tcgatttgtc acaggttccg ccttacctgc
    19501 gccccatcaa tatgatgttt cagtcttacg cgctgtttcc gcatatgacc gtggaacaga
    19561 acatcgcttt tggcctgaaa caggacaaac taccgaaagc ggaaattgtc agccgggtca
    19621 atgagatgct cggcctggtg catatgcagg agttcgccaa acgcaaaccg catcagcttt
    19681 ccggtggtca gcgacagcgt gtggccctgg cccgaagcct tgccaagcgc ccgaaactat
    19741 tactgctcga tgagccgatg ggcgcgctgg ataaaaagct gcgtgaccgg atgcagcttg
    19801 aagtggtgga tattctggag cgcgtcggtg tgacttgtgt gatggtcacc cacgatcagg
    19861 aagaggcgat gaccatggcg gggcgcatcg ccattatgaa tcgtgggaaa tttgtccaga
    19921 ttggcgaacc agaagagatc tacgaacatc cgaccacccg ctacagtgct gaatttatcg
    19981 gttcggttaa tgtctttgaa ggtgtactca aagagcgtca tgaagatggc ctggtgcttt
    20041 attcgccggg gctggtgcat ccactgaaag tcgatgtaga tgcttcggtg gtcgataatg
    20101 tgccggtgca tgtggcgctg cgcccggaaa aaatcatgct ttgcgaggaa cctcccgctg
    20161 acggttttaa ctttgcggta ggtgaggtga tgcacattgc ctatctcggc gatctttcgg
    20221 tgtatcacgt tcgtctcaag agcggacaga tgatcagcgc tcagctacaa aacgctcatc
    20281 gatatcgcaa agggttacca acctggggcg acgaagtgcg tttgtgctgg gaagtggaca
    20341 gctgtgtggt gctgacggtt taaggagcaa agatgagtac acttgaacct gctgcccagt
    20401 cgaaaccgcc gggcggattt aagctgtggt tgtcgcagct gcaaatgaag catgggcgca
    20461 aactggtcat tgcgttgcca tatatctggt tgatcttgct gtttctgctg ccatttctga
    20521 ttgtctttaa aataagcctg gcggagatgg cgcgcgctat tccaccttat accgaactga
    20581 tggagtgggc tgacgggcaa ctgtccatca ctcttaatct cggtaatttt ctgcaactga
    20641 ccgacgatcc gctctatttc gatgcttatc tccagtcgct acaggtggct gcgatttcga
    20701 cattttgctg tttactgatc ggctatccgc tggcgtgggc ggtggcgcac agtaagcctt
    20761 cgacccgtaa tattttatta ctactggtga tcctgccgtc gtggacctcg tttctgatcc
    20821 gcgtttatgc ctggatggga atattaaaaa acaacggtgt gctgaataat tttctgctgt
    20881 ggctgggggt tatagaccag ccgctgacca ttctgcatac caatctggcc gtttatatcg
    20941 gcattgttta cgcttacgtg ccgtttatgg tactgccgat ttataccgcg ttgattcgta
    21001 ttgattattc gctggtggaa gcagcgctgg atctcggtgc acgaccgctg aaaacgttct
    21061 ttactgtgat cgtgccgctg actaaaggtg ggattattgc cggatcgatg ctggtgttta
    21121 tcccggctgt gggcgagttt gtgatcccgg aactgctcgg tggcccggac agcatcatga
    21181 tcgggcgcgt gctatggcaa gagtttttca ataaccgcga ctggccggtg gcctcggcgg
    21241 tagcgatcat catgttgctg ctgctaattg tgccgataat gtggttccac aaacaccagc
    21301 aaaaaagcgt gggagaacac ggatgaataa tttaccggta gttcgttcac cctggcggat
    21361 tgtgattttg ctgctgggct ttacctttct ctacgcgcca atgctgatgc tggttatcta
    21421 ttcgtttaac agctcgaagc tggtgacggt gtgggccggc tggtcaacgc gctggtatgg
    21481 tgagttattg cgcgatgacg cgatgatgag tgcggttggt ttaagcctga caattgctgc
    21541 ctgtgcggca acggcggcgg cgatcctcgg gactattgcg gcggtggtgc tggtgcgctt
    21601 tggcaggttt cgcggatcaa atggctttgc ctttatgatc accgcgccgc tggtgatgcc
    21661 agatgtcatc acgggcttgt cgctgttgtt attattcgtc gcgcttgctc atgccattgg
    21721 ctggcctgcg gaccgcggta tgctcaccat ctggctggcg catgtcactt tttgtacggc
    21781 ttatgtggcg gtcgttattt cttcaagatt gcgggaactg gatcgctcga tagaagaagc
    21841 agcgatggat ctcggtgcga cgccgctgaa agtgtttttc gtcattacgc taccgatgat
    21901 catgcccgcg atcatttctg gctggttact ggcttttact ttgtcgcttg atgatctggt
    21961 gatcgccagc tttgtttccg ggccgggagc caccacgtta ccgatgctgg tcttttccag
    22021 cgtgcggatg ggggtgaatc cggaaatcaa cgcccttgca acgttaattc tcggtgcggt
    22081 cggaattgtc ggatttatcg cctggtatct gatggctcgc gcagaaaaac agcggatacg
    22141 cgatatccag cgtgcaagac atggctgaag acactaaaat ttgccaacct ggctacataa
    22201 tgccgcgcat gccgcggcat tgttttcatg gaagacgaaa cgttgggatt ttttaagaaa
    22261 acatcttcat ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtggc ggcgctcgcc
    22321 attattatga tccgtggcct cgacgtgctg atgattttca atacgctggg cgtgcgcggt
    22381 attggcgagt tcattcatcg cagcgtacaa acctggagtt taacgctggt ctttttaagc
    22441 agtctggtgc tggttttcat tgagatctgg tgtgcgtttt cactggtgaa agggcgtcgc
    22501 tgggcgcgct ggctatatct gctgacacaa atcaccgccg caagttactt gtgggcggct
    22561 tcgctggggt acggttatcc ggagctgttc agcattcccg gtgaatcaaa acgtgaaatc
    22621 ttccatagcc tgatgctgca gaagctgccg gatatgctca tcctgatgct gctgttcgtt
    22681 ccctcgacca gtcggcggtt cttccagttg caataatgtg tataatcgtc gcccctgatg
    22741 atgtgaaggt caatgtatgc agtgcgcact ttacgacgcg ggtcgctgtc gttcctgtca
    22801 gtggataacg cagccgattc cagagcaact ctccgctaaa accgccgatc ttaaaaatct
    22861 gctcgccgat tttccggttg aggaatggtg cgcgccggtg tcaggcccgg aacaagggtt
    22921 tcgtaataaa gccaaaatgg tggtgagtgg tagcgttgaa aaaccactgc tcggtatgct
    22981 gcatcgcgac ggcacaccag aagacctttg tgactgcccg ctttatcctg cctcatttgc
    23041 gcccgttttt gcggcgctaa aaccgtttat cgcccgtgcg gggctgacac cctacaacgt
    23101 ggcgcgtaag cgtggcgaac tgaaatacat tctgctgact gaaagccaga gcgatggcgg
    23161 catgatgctg cgctttgtac tgcgttctga aaccaaactg gcacaactgc gtaaggcgct
    23221 gccgtggtta caggaacaac taccgcaact gaaagttatt accgtcaata ttcagccggt
    23281 acatatggcg attatggaag gggagacgga gatctacctt actgaacaac aggcgctggc
    23341 agagcgtttt aatgacgtac cgctgtggat ccgtccgcaa agtttcttcc agactaatcc
    23401 ggcggtcgcc agccagctgt acgctaccgc gcgcgactgg gtacggcagc tgccagttaa
    23461 acatatgtgg gatctgttct gcggtgtggg tggctttggt ttacactgcg cgacgcctga
    23521 catgcagtta accgggatcg aaattgcatc agaggccatt gcctgtgcaa agcagtcagc
    23581 cgctgaactg ggcttaacgc gtttgcaatt tcaggcgctg gactccactc agtttgccac
    23641 cgctcagggg gaggtgccgg agctggtgct ggttaatccg ccgcgccgcg gcattggtaa
    23701 accgctatgt gattatctct caacgatggc accgcgtttt attatctact ccagctgtaa
    23761 cgcccaaacc atggcgaaag atatccgcga actgccaggt taccgcatcg aacgggtaca
    23821 gctttttgat atgttcccgc acaccgcgca ctatgaagtg atgacgctgc tggtgaagca
    23881 ataaaaaagc cgcaggtgcg gcttcagatt gctgatcagg tgcgctttgt tcatgccgga
    23941 tgcggcgtgt acgccttatc cggcctacaa aatcatgcta aatcaataca ttgcagagtt
    24001 gatgtgggcc agataagcgc agcgcatcag gcaatcttac ttttgtcatc agtctcaatt
    24061 actgcgggaa ccactggtca ttgattttct gatatgtacc gtcagcttta attgctgcca
    24121 gcgcgttatt cagtttttcc agcagagctt tgttatccgg acgtacagcg atacccaggc
    24181 cggtgccgaa gtattgcgga tcggtcactt tttcagtcgc aacgcccagt tgcggattgg
    24241 ttttcagcca ttcgtttacc accgctgtgt caccaaatac cccatcaata cgaccatttt
    24301 tcagatcgat aaaggcattc tgataactgt cataggagac agttttcact tcggggtgct
    24361 gatcctgaat atatttctgg tgcgtggtgc cgttttccat cccaatacgt ttgcctttca
    24421 gatcggcaaa cgttttgtag gtatcttttt tggcaatcac gacggctgag ttttcatagt
    24481 agggcgtggt aaacgatacc tgcttgctac gctcgggcgt gatatccata ccggagatta
    24541 cggcgtcata ttttctgaat ttcagtgacg ggatcaggct gtcgaacgcg tgattagtaa
    24601 aagtacattc tgcctgcatt tgtttgcaca aggcttttgc cagatcgata tcaaagccga
    24661 caatctcatt attagcacct atagattcaa agggtggata ggtggctgaa acgccaaaat
    24721 tgattttctc tgcggcagaa gcaccgaaag taaaggaagc aagtaaagcg gcaagaacta
    24781 actttttcat gatggaactc ccgtctgtca atcttatgat ttttggccgt gtctgcggca
    24841 tgggataaca atgccatcaa gtgaatttat atgcaataaa tatgattaaa taatttaaat
    24901 gaaataaaaa aagacggata acttagtgag ttgtccgtct tcattataag aatttatgca
    24961 ctatgtaggc cggataaggc gtccacgccg catccggcac aggcaccgtg ctgatgtctg
    25021 atgcgacgct ggcgcgtctt atcagaccta caaacccccc cggcgaatgt acgcagctac
    25081 attaattccg ccgttcgaat gccagtgctt tgcgctcgat cagacgcatc atcagcgtca
    25141 gcaggccgtt gacgaccagg taaataatcc ctgccgcacc gaacaccatt acatcgtagg
    25201 tgcgtccgta caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca
    25261 gagaggtact tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa
    25321 aggcatacgg cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg
    25381 actgccactg accttctggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg
    25441 ccgcactgtt cagcgataac gcaatcagcg cacataacca cggttctgac aacaaatgcc
    25501 acagtgccgg atactcctgc aaagtcggaa actggcccgg cccgtaataa atcaggaaga
    25561 tctgcaccag cagcggcgta ccggtaaaca gcgtgatata accccgcacc agccacacca
    25621 gcaccggcgt tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa
    25681 tcagcgaggc aacggttagc gtcaggctgg tgtgtagccc tttcatcagt tcgggtaaat
    25741 actcaaacat tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag
    25801 aatgtactga ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tgtaccaggt
    25861 aaatggttcc tgggtacgag tagcgatgct ttttgtttgc agcattaaat cattcacact
    25921 aatcaaactg accagcgcgg tatctttcag cagcaccagc cactggttac cgaggccagg
    25981 cagcgcatga cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag
    26041 ccccagcgcc tgaccagact cccactgacc caccggcacc gctttcaacg cgccccgaag
    26101 cgtttgcgag gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg
    26161 gctaacgtcg aagttctcaa tgtccatctg cactgggatc tgcacgaacc caagattgat
    26221 agtgaagcca tccgaaagcg tcagcagcag ctgcgaggag ccaaaataga taaacagcac
    26281 caccagaatt tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac
    26341 aggacgccat tttgccgact cccataccgc aaagaacatc gccagcgcca gcccgacaat
    26401 caatgcacaa acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc
    26461 attcatcagg aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt
    26521 cttcactttt tccagcgcag tgttgagttt ctgctgcagc tcagtgttgc cctgacgtac
    26581 cgcgatgccg aggccagtgc cgaagtaatc tttatcggtc actttgtcgc ccaccgccgc
    26641 cagtttcggg ttatctttca gccactcagt gaccactgcg gtgtcaccga agacgccgtc
    26701 gatacgcccg ttttgcagat ccagttttgc gttctggtag ctgtcatacg gaacggtagt
    26761 gatttccggg tgcttatcca taatgaattt ctggtgtgtc gtcccgttct gaacgccgac
    26821 ttttttgcct ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc
    26881 agagttgtca tagtacgggg tggtaaacag tacctgcttt tcacgctccg gagtgatatc
    26941 catgcccgcc atcactgctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa
    27001 cgcctggtta gagaaagtac aggttgcatc aatctcttta cacagtgcct gtgccaggtc
    27061 gacgtcaaaa ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggaggc
    27121 ttcggtagca aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa
    27181 cgcggcaatc agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt
    27241 ttaaatgctt cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga
    27301 ccattttcca tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg
    27361 acgatcacct gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc
    27421 gtaatttccg ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc
    27481 atcaacgcac gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa
    27541 cgatcgctat aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc
    27601 tgatctttac tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc
    27661 agatgcggcc acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga
    27721 atcgctttgt cagagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta
    27781 ccggagcgcg gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg
    27841 ctggggccaa gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc
    27901 gcctgatgcg cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt
    27961 atactggcag tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc
    28021 aatatttctg ctttaaaagt taaaagcaaa gcgcattatt caataaacat agcacaaaat
    28081 aacgggggcg gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt
    28141 tacggggttt attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc
    28201 cgccgtggca tgaacgctgc cgcctaaata gcgtacatcg tcgatcaccc aacactggcc
    28261 ttcctgaatc attaacactt catcctgcca gctctgatca ccctgtttta gatccacacg
    28321 cagaggaata tttcgcgcat cacgattcgg gattgttgag gcactggcaa cacgggcgct
    28381 ctctggcaag gttgtgcgac tggagaatgg atcgttggtc agtagttccc gatggttatt
    28441 atcccgggag gcatcgctaa gcagtgtcgc cagtttgtcg ctcagataag ggcgcaaggc
    28501 ggtgatgtcg ttgctgcggt gcaaaatgcg gtagtcataa aattgctggg ccacgttatc
    28561 cgggcctcct tcaacgcaag gaccactgcg tgtgccgtta tctttataag ctggagtgac
    28621 tgtggtgcag gcactgagga gcagtgcgca ggggataagc attgtcaatt tgctgtagcg
    28681 cataatgatt tccttataag cgatcgctct gaaagcgttc tacgataata atgatatcct
    28741 ttcaataata gcgtatcagt ctgataatgc ttttgagatc gaaggcttag caaacaagga
    28801 gatcgatcat gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt
    28861 gcggtgtggt gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgctg
    28921 gtatccgcga tatcgttggc ggacgctccg gtgcctacga aaaagaatta cgtaaagcgc
    28981 gggagatcgc ctttgaggag ctcggctccc aggcgcgggc gctgggggcc gatgccgtag
    29041 tcggtattga tatcgactac gaaacggttg ggcaaaacgg cagtatgctg atggttagcg
    29101 tcagcggtac ggcggtgaaa acgcgtcgat gagaagagtc ttctggctga ttgccgccgc
    29161 tctgttactg gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct
    29221 tgatacccga cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta
    29281 caccgcagat gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca
    29341 ttatctggtc cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt
    29401 gccggaacaa gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct
    29461 taacgacacc tctattggca ttgagctgga aaaccgtggc tggcaaaaat cggccggagt
    29521 gaaatatttt gccccgtttg aaccggcaca gattcaggcg cttattccac tggcgaaaga
    29581 tattattgcc cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcacc
    29641 gcagcgcaaa gacgatccgg ggccattatt tccctggcag caactggcgc agcaggggat
    29701 tggtgcctgg ccggatgcgc agcgggttaa cttttacctt gccgggcgcg cgccgcacac
    29761 accggtagat accgcgtcat tgctggagct tttggcgcgc tacggttatg acgttaaacc
    29821 tgatatgaca ccgcgcgagc agcggcgcgt gattatggca ttccagatgc atttccgccc
    29881 gacgttatat aacggcgaag cggatgcaga aactcaggcg attgccgaag cattgctgga
    29941 aaaatacggg caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt
    30001 ctcgatacct tcatccaggg tgatgaccgg ctgataacct aactcttcct gcgcacgcgt
    30061 aatatccagc gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc
    30121 ttttgctgac ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta
    30181 ggggacggaa cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct
    30241 gcgcagtgtg cgatgctcgc cgttggtgat gttgtacaca cgcccggaag gtagcttatc
    30301 gcaggcttcc tggcttgcca gccacatggc gtgcacggca ttttcatagt aggtcatatc
    30361 gaccagcgca ctgccgccat gcggtaacag aatactgccg tagtggtgca tcatatgcgc
    30421 cagacgggga ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt
    30481 aaagcgcgtt tgtggattcg cctgcgaaag catattgatc acttcttcgc tggctgcttt
    30541 gctgcgggca aactcgttgg cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg
    30601 gtggtgataa tcgaagtaca gggaggggga agagatatga ataaagttac gcacacccca
    30661 ggcgacagcc cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg
    30721 ttgtgtcccc cagggtgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag
    30781 catcacttta gcttgtgatg aaaccagctc ggtcagatcc gccggaacaa actctgcgcc
    30841 cattttttcc agcaatttgc ccattgcctc gttgcgaccg gtcgctcgca cgctgatgcc
    30901 tttctggcat aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag
    30961 taccttcata tcaatccact gttgttgaga aaataacgtg cgcattcttc cgtgatttcc
    31021 cccatgatgc aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt
    31081 tgttctgcca gtctggcgat ttgttttgcc attccgcgaa aaataaacag atgcgcgggg
    31141 atcatcaata accagtaaaa cagccccggc ataccgtgcg gatgccagaa agcgcggaca
    31201 tcgatagtac gatagtcgcc tttatcttcc aggctaaaac acagtcgtcc cagccccggc
    31261 gctttcatgc caaataacaa cgtaagttgt ttttccggtt caacgacaat cactttccag
    31321 ctatctaccg catcgccagt ctgtaaatat tcgcgctccg ggcggccttt cgccagctta
    31381 tgaccgatcg cgcggtccat caacgcccgt gtctgccaca aaatattgcc aaagaaataa
    31441 cgctctttac cgccgatttg gttcactacc tgccataaag cagcaaggct ggcggacgtt
    31501 ttaacggtaa accccgcctg tttggcaaaa taaccgtact ccggtcgcca gcgggcaaag
    31561 gcctgagcgt cgtagcccca gtcgctggag ttgaccagtt tttcctcctc tttcaacgtg
    31621 ctacgtaccg cgtcatcgaa agcgatcagc cgttgtggga tgagtgcacg tagcgcggta
    31681 tcatccgcca gcagatcgtg tttcagcccc tgaatcaacg ccctggcggt ggtgggcggt
    31741 acggaagtaa tcacattgag aaaccacacc gaaatccagc gggtggggag ggggatgggg
    31801 atcaaccagc ggcgcttacc gctcaccgcc ataaaatgtt caaactgttg ctgataactg
    31861 agcacctctg gtccggcggc ttcgaagatg cggtgttcgc tggccggatg atctaacaac
    31921 gccaccagat agtgcagcaa gttttccagc gcgatgggcg tggtgcgtga acgtacccag
    31981 cgtggcggcg ttaacactgg caggttgtag accatatcgc gcatgacttc gaacgccgct
    32041 gaacctgcgc caacgataat tccggcccga agttcggtca caggtacatt cgcttcacga
    32101 agaatgtccg ccgtagcctg acgagcacgc agatgatccg actgctcatg tggcggggcc
    32161 tgcaacgaac tgagaaagat taattgctta actggtactt cacgtagcgc atcgcggacg
    32221 ttgagagcca cctggcgctc ctgagcgata aaatcgccgc cttcgcccat gctgtgcacc
    32281 agaaaataga cggtatcgat atcctgcaac agggccggaa ggttatccgg ccagctgaga
    32341 tcgactttat ggcaactgac gtttgccagt tgcagctttg caagcctgtc gacatgacgt
    32401 gccgccgcca ggatctgatg cccttgctgg ctgagtgtgc gcaccagatg ctgaccaatg
    32461 tagccactgg caccgagaac taaaatgcgt tgcggcacgt ctctctcctt aacgcgccag
    32521 gaatgcgcgc cagtgggcgg cgacttccgc cagttgttcg cgcgagacgt caagatgcgt
    32581 caccaggcgg acaatcggcg aggcgttaat cagcacgttt ctcgctttca tgtattcgcc
    32641 taacgcggca gcattttctt ccccgacgcg aacaaacagc atattagtgt cctgacgcat
    32701 cacatccgcg cctgcttcac gcagttgctc cgccatccag gcggcgttgt cgtgatcttc
    32761 ctgcaaccgt gcgacgttat ttttcagggc atacatcccg gcggcagcca gaatcccgga
    32821 ctgacgcatc ccgccaccgg ccattttccg ccagcggatg gcacgtttaa tgtaatcacg
    32881 attaccgacg agtaatgaac cgactggcgt cccaagacct ttcgacaggc aaatggtgaa
    32941 cgaatcacaa tattgcgtga tctctttcag ttcgcagccg taagccacca cggcattaaa
    33001 gatgcgcgca ccgtcaacat gcagcgccag attgcgctcg cgggtaaatt cccatgcttc
    33061 tttcaggtat tcacgcggca gcactttgcc gttgtgggtg ttttccagac tgagtaattt
    33121 ggtgcgggcg aaatggatat cgtcgggttt gattttcatc gccactttat ccagcggtag
    33181 cgtgccgtcg gcagccgcgt cgatgggttg cggctgaata ctgcccagca ccgctgcgcc
    33241 accggcttca aacagatagt tatgcgcggc ctgaccgaca atatactctt cgccacgttc
    33301 gcagtgactg agcagagcga ccaggttggc ctgagtgccg gtaggcagaa aaatggcggc
    33361 ttctttaccg gaaagctctg ctgcatagtc ctgcagagca ttaacggtag ggtcgtctcc
    33421 gtaaacgtcg tccccaaccg gggcggccat catcgcttcg agcatggcgc ggctcggtcg
    33481 ggtaacggta tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca
    33541 ccctttttac cttagccagt tcgttttcgc cagttcgatc acttcatcgc cgcgtccgct
    33601 gataattgcg cgcaacatat acaggctaaa acctttggcc tgttcgagtt tgatctgcgg
    33661 tggaatggct aactcttctt tggcgaccac cacatccacc aacaccggac cgtcgataga
    33721 gaaggcgcgt tgcagggctt catcaacttc agacgctttt tctacacgga tacccgtaat
    33781 gccgcacgct tcggcaatgc gggcaaagtt tgtgtcgtgt agttcggtgc cgtcagtcaa
    33841 atagccacca gctttcatct ccatcgccac aaagcccagc acgctgttgt taaagacgac
    33901 aattttcact ggcagtttca tctgcactac tgagaggaaa tcgcccatca acatgctaaa
    33961 accgccatcg ccgcacatgg cgaccacctg acgttctggc tctgtcgcct gcgcacccag
    34021 cgcctgcggc atggcgttag ccatcgaacc gtggttaaac gaacctaaca ggcgacgctt
    34081 gccgttcatt ttcagataac gtgccgccca taccgttggc gtgccgacgt cacaggtaaa
    34141 gatggcgtca tcggcggcaa aatgactaat ttgctgcgcc agatattgcg ggtgaatggc
    34201 tttctcgctc ggtttagcta aatcgtccag ccctttgcgg gcatcgcggt aatcttccag
    34261 cgctttatcc agaaacttgc gatcggtttt ttcttccacc agtggcagca atgcgcgcag
    34321 ggttgatttg atatcgccga ccagcgccat atctaccttg ctatgcgcgc cgatgctggc
    34381 tggattgata tcaatctgaa taattttggc atcggtcggg tagaacgcac ggtaggggaa
    34441 ctgagtaccg agtagcacta acgtgtcggc gttcatcatg gtatggaaac ctgacgagaa
    34501 gccgattaac ccggtcattc caacatcata cggattatcg tattcgacat gttctttacc
    34561 gcgcagggca tgaacaatag gcgctttaat tttcccggca aactcaacta actctttatg
    34621 cgcccccgcg cagccgctgc cacacatcag ggcgatattg ctggaataac gcagcagttg
    34681 cgccagtttg cgtaactctt cttcttccgg cgtcacgatt ggttgtggcg catgatacca
    34741 gtgcgtggtt gccccttctg gcgcaggttt taacgccacg tcgcctggta acacgacaac
    34801 cgaaacgcca cggttaagca ccgctttgcg catggcaatc gccagtactt gtggaatctg
    34861 ctccgggctg gaaaccagct cgcaatagtg actacattcg cggaatagct cttgtgggtg
    34921 ggtttcctgg aaatagccgc tgccaatttc gctggaggga atatgagcgg caatcgccag
    34981 taccggaacg tgattgcggt ggcaatcgaa caggccgttg attaagtgca ggttgccggg
    35041 gccgcacgat ccggcacaga ccgccagttc tccgctaagt tgtgcttcag cgccagcggc
    35101 aaaggccgcc acttcttcgt ggcgggtgga catccactcg atggtgccca tgcgattaag
    35161 actgtcacta agaccgttca gagagtcgcc tgtgactccc cagatgcgtt tcacccctgc
    35221 cgattcgagt gttttggcga tataagctgc aaccgtttgt ttcatggttc tccatctcct
    35281 gaatgtgata acggtaacaa gtttagttca tctgacggag ggggaaggga tgggagagaa
    35341 aggaggcact aacggttaaa tagcccgatg aaaggaatat catcgggcat aaggcgatta
    35401 tgcgagaacc aaatccccct gcggatggca ggagcaggcc agtacgtaac cttcagcgat
    35461 ttcggcgtcg gtcagcgtca ttgtactgct caccgtatat tcaccggaaa ccacttttgt
    35521 cttacagcag ccgcaaacac ccgcacggca ggcagcgaca accggaacgt tattgctttc
    35581 cagcgcctcc agtagcgtgg tgccaaccgg ggcgtaaaat tctcgtgccg gttgcagttt
    35641 ggtgaatttc agaccgctgg tcgccgcttc tgctactggg gtgaagaatt tctctttaaa
    35701 gaaacgcgtc acgccgagcg ctttcacttc ctgctctacc caatccatat acggagccgg
    35761 gccgcaggtc atcacggtac gtgaagctaa gtcaggtaca cctgccagca gttcgcgagt
    35821 gagacgacca gcgataaagc cttcggtaac gttattttct gccaccagcg ttaccggata
    35881 gttacgccac tcatcggcga aaataacatc ctgcggcgta cgcacgttgt agatcacccg
    35941 cacatcggcc tgtggacggt tcttcgcaag ccagcgacgc atcgacataa tcggcgtgac
    36001 gccgcagcct gccgccagca acaggaattt atcttctgct ttatcgtcgc aggtaaattc
    36061 ccccatcgcg tccgaaagcc agagataatc accgcgtttt acatcgcgcg tcagccactg
    36121 ggagccgaca ccgtcatcaa tccgccgcac ggtcagggtg atatattcac tcacgcctgg
    36181 cgtggaggaa atggtgtaag cacgcagcgt ttccgctgag tttcgcacgc tgaccagtgc
    36241 atattgcccg gcgcgatatg ggtagtaatc gtggcaaatc agggaaatcg tccacacatc
    36301 cggcgtttct tgcgtaatgt gatgaacctg catccgccac gggcattgat tcgttggcat
    36361 cgtcatcgac aaactcctta cgcgctcaac agttgcttca tgtcttcttc aacagtggtg
    36421 atagaacgca ggccaaattt ctcgttcagc accgccagca ggtctggtgt caggaaacca
    36481 ggtgcagtcg ggccggtgac gatatttttc acgcccagag aaagcagcgt cagcagaatg
    36541 acgatcgctt tctgttcaaa ccaggagagc accagcgaca gcggcagatc gttgacaccg
    36601 cagcccagtt tctctgccag agtgacagcc agaataatcg ctgagtaagc atcgttacat
    36661 tgacctgcat ctaccagacg cggcagacct tcgatatcgc caaactcaag tttgttaaag
    36721 cgatatttac cacaggcgag ggtcaggatc aggcagtcat ccggcacgct ggtggcgaaa
    36781 tcggtgaagt agtggcgctc gccgcgtgcg ccgtcacagc caccaagcag gaagatatga
    36841 cgcagttttt cacggctcac cagatcaatc agcgtatccg ctgcgccaag cagcgtctgg
    36901 cggccaaagc ccacggtgat caggtgcgga atttcgctgt acgggaagcc cgccatttgt
    36961 tgcgcctggg cgatgaccgc agaaaaatct tcgccatcca gatgacgcac acccggccag
    37021 ccaacgatgc tgcgggtcca gatacgatcg tcataagcgc ctacggttgg gtcgatgatg
    37081 cagttcgagg tcatcacgat ggggccaggg aaacgagcga actccacttg ctgattctgc
    37141 cagccgctgc cgtagttacc gaccagatgc ttgaatttac gcagctccgg gtagccatgc
    37201 gcaggcagca tttcgccgtg ggtgtaaaca ttaacgcccg tgccttcggt ctgttccagc
    37261 aggttgtaga gatctttgag atcgtgaccg gaaatcagga tgcatttacc cgccgtcgct
    37321 ttgacgttga cctgggttgg cgtcgggtga ccgtatttac cggtttcgcc tgcatccaga
    37381 atgctcatca ctttgaagtt catctggccg atttccattg aacactcaag aagcgcgttc
    37441 atatcggcag gccaggtccc cagccacgcc atgattttat gatactgggc ataaatatcg
    37501 ttgtcgtatt gaccgagaac atgcgcgtgt tccatatagg ccgctgcacc tttcaggcca
    37561 tacaggcaca gcagacgcag gccgagaatg ttttcgccaa tcgccgcttt atctttgtta
    37621 ggggtaaatt ctgctgcctg acgttgcagc tcgccgagat cgtcgctcac cagttgcagg
    37681 tcagccatcg ggttatcgac gcgcgcgttg gcatctacag ccaggcattg cgctttcagc
    37741 gcctcgcgca gggcaatcgc ttcacgagcg tagccgacaa tacgcggaga atcgaagtta
    37801 acgttggtca gggttgagaa aaaggcacgt ggcgcgaagc tgtcaacatc gtggttgatg
    37861 atgccgtatt cacgcgcttt taccgcccag gcagaaagcc cttgcagcgc cgcgatgagt
    37921 aaatcctgaa ggtcagaagt ttccgccgtt ttaccacaca tcccctgcgc gtatgagcag
    37981 ccgtttcctg ccggagtacg gatagtttgt tcacattgca cacaaaacat gatcacacct
    38041 tttaaagtta tatttaatat acatgtttaa ggttaagacg cttaacgcgg ggataaaagg
    38101 gatttttcat gcaactttaa gggagattga tttagcgcaa ttttggcggc agggatctac
    38161 cgccagagag gtattacgca gagaaaaagg cgatgaggat cggcactaac aggctaagaa
    38221 taaaaccgtg aacaattgcc gccgggacca tatccagccc gccagtacgt tgaagaacgg
    38281 gcagggtgaa atccattgat gtggcaccgc ataagcccag tgcagtagag cggctgcggc
    38341 gaatcagccc agggatcaac ataatagcaa tcagttcacg ggccagatca ttaaaaaacg
    38401 ccgcgctccc gattaccgga ccaaaagatt cggtcaataa aataccggaa agagaatacc
    38461 agccgaaacc ggaggccatt gccagcgcgg tattgatggg gagatcaaga ataaaggcgt
    38521 taattaaacc accaattaat gaactgacaa ccaccaccac ggcgacaatc attccccggc
    38581 gattaaggac aatctgcttt aaggtcatgc cattattgcg caactgaata ccaacgagga
    38641 aaagtagcaa aattaacgtg tattcactgg cttcggtcgc gtgttgtaag aaagccagtc
    38701 cactcagacc aatggcaaaa ccaatcacta ctacgccgca cagttttagc gactccagcg
    38761 ccatcgcaat acgcgacggg agtttttctt gctgatggtg gttgcgccac ggcaggcctc
    38821 gctccagcca catcagggcg gcaatattac acagtaaaat aacggtaata ctgacggcag
    38881 aataatgcag aatcgccaac aggttactgg cgaggttatc gagaaacgcc agactgatac
    38941 ccataaaaaa gagaataagg taaaccatcc agcttaatag ctgattaata acttttaacg
    39001 cagcttgttg gcgaagcgga atgaggtaac ccacaatcag gggaaccaga atgattaaca
    39061 gcccagaaaa catgaaaacc cagtccttgc aaagatgaag tcgaaatgcg cgatgacaca
    39121 ctactgaaag cggaaggacg agtaaagttg caattaaaag gaaatgttat gcataaggag
    39181 cagtagagta ttcgttttca tttaaagata ttcttgcgct ttaattacaa actgcaccga
    39241 tgttggtggc gtcaaaatcg ccgaggcgtt ccctgaaggc cggggcagcc cacatggatg
    39301 tgggctgagg gcgcgtttta cagggatgtt acctcgcgcc cgacccggta gccgtaaggg
    39361 ataagtcgag ggcaccgcgc agcggcgatt ttgttcgcca gagcccgggg gtgcaggggg
    39421 cggcggcgat tggccgcccc ctgcgcgctc cttgcgccag tggcaatatg tggcttagct
    39481 catgaaagga gcgcaacaag atgatgaatc aacatataac aacatcttaa aaaaaggcct
    39541 gacattacgc caggccttct gcgttaatta atcacgcttt tccagcaggg tccggtaaat
    39601 cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca gttgttctaa
    39661 tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa cagaagtgtt
    39721 agtcaccgga atactaatta agtgaatcag ggttaaggcc agaccaatag cgatcggcgc
    39781 aaaacctgcc ggcgcgaatt tgtcggttgt gccgtggatc accaacagga aacctgcact
    39841 caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggtg aatgctcgcc
    39901 ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac cactggcaat
    39961 taaatacagc agcgccgctg caacaatacc gccgacaacc tgggcaatta cgtagccaac
    40021 gacttctttt gccggaaaac gtccgccagc ccataaacca atagtgaccg ccgggttaaa
    40081 atgaccacca gaaatatgac caacagcaaa ggccatcgtc agaacggtca gaccgaacgc
    40141 caacgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca gtacagcact
    40201 accacagcca ccaaaaacaa gccagaaagt accaaaacat tcagctgcta attttctgaa
    40261 catatccacc tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc
    40321 gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa aaacgaaggc gagtcattca
    40381 ccagataaat aaatccagta aatttgattt agggcaacag cgggttgccc catatagtca
    40441 tttgtctgat tgacagtgta gtgcaagcaa aagatttaat cctttaggcg taataaaaaa
    40501 taa
//
LOCUS       DAFAVF010000023        40219 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFAVF010000023 DAFAVF010000000
VERSION     DAFAVF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.023"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            144..1325
                     /gene="tsgA"
                     /locus_tag="LGK42_001216"
     CDS             144..1325
                     /gene="tsgA"
                     /locus_tag="LGK42_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBK2657490.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            1587..4130
                     /gene="nirB"
                     /locus_tag="LGK42_001217"
     CDS             1587..4130
                     /gene="nirB"
                     /locus_tag="LGK42_001217"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBK2657491.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4127..4453
                     /gene="nirD"
                     /locus_tag="LGK42_001218"
     CDS             4127..4453
                     /gene="nirD"
                     /locus_tag="LGK42_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBK2657492.1"
                     /translation="MNQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            4580..5386
                     /gene="nirC"
                     /locus_tag="LGK42_001219"
     CDS             4580..5386
                     /gene="nirC"
                     /locus_tag="LGK42_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBK2657493.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            5405..6778
                     /gene="cobA"
                     /locus_tag="LGK42_001220"
     CDS             5405..6778
                     /gene="cobA"
                     /locus_tag="LGK42_001220"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBK2657494.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            7033..7200
                     /locus_tag="LGK42_001221"
     CDS             7033..7200
                     /locus_tag="LGK42_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBK2657495.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            7444..8832
                     /gene="frlA"
                     /locus_tag="LGK42_001222"
     CDS             7444..8832
                     /gene="frlA"
                     /locus_tag="LGK42_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBK2657496.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            8853..9875
                     /gene="frlB"
                     /locus_tag="LGK42_001223"
     CDS             8853..9875
                     /gene="frlB"
                     /locus_tag="LGK42_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBK2657497.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            9925..10755
                     /gene="frlC"
                     /locus_tag="LGK42_001224"
     CDS             9925..10755
                     /gene="frlC"
                     /locus_tag="LGK42_001224"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBK2657498.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            10752..11537
                     /gene="frlD"
                     /locus_tag="LGK42_001225"
     CDS             10752..11537
                     /gene="frlD"
                     /locus_tag="LGK42_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK2657499.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            11637..12368
                     /gene="phoB"
                     /locus_tag="LGK42_001226"
     CDS             11637..12368
                     /gene="phoB"
                     /locus_tag="LGK42_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBK2657500.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(12520..13605)
                     /locus_tag="LGK42_001227"
     CDS             complement(12520..13605)
                     /locus_tag="LGK42_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK2657501.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(13617..14921)
                     /locus_tag="LGK42_001228"
     CDS             complement(13617..14921)
                     /locus_tag="LGK42_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBK2657502.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(14933..15286)
                     /locus_tag="LGK42_001229"
     CDS             complement(14933..15286)
                     /locus_tag="LGK42_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBK2657503.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(15297..16175)
                     /locus_tag="LGK42_001230"
     CDS             complement(15297..16175)
                     /locus_tag="LGK42_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBK2657504.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(16172..17398)
                     /locus_tag="LGK42_001231"
     CDS             complement(16172..17398)
                     /locus_tag="LGK42_001231"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK2657505.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(17398..18561)
                     /locus_tag="LGK42_001232"
     CDS             complement(17398..18561)
                     /locus_tag="LGK42_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBK2657506.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(18645..19007)
                     /locus_tag="LGK42_001233"
     CDS             complement(18645..19007)
                     /locus_tag="LGK42_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK2657507.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(19024..19929)
                     /locus_tag="LGK42_001234"
     CDS             complement(19024..19929)
                     /locus_tag="LGK42_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657508.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(20198..21202)
                     /gene="trpS"
                     /locus_tag="LGK42_001235"
     CDS             complement(20198..21202)
                     /gene="trpS"
                     /locus_tag="LGK42_001235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK2657509.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(21195..21953)
                     /gene="gph"
                     /locus_tag="LGK42_001236"
     CDS             complement(21195..21953)
                     /gene="gph"
                     /locus_tag="LGK42_001236"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBK2657510.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHAADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(21946..22623)
                     /gene="rpe"
                     /locus_tag="LGK42_001237"
     CDS             complement(21946..22623)
                     /gene="rpe"
                     /locus_tag="LGK42_001237"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK2657511.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(22641..23477)
                     /gene="dam"
                     /locus_tag="LGK42_001238"
     CDS             complement(22641..23477)
                     /gene="dam"
                     /locus_tag="LGK42_001238"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBK2657512.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(23584..24870)
                     /gene="damX"
                     /locus_tag="LGK42_001239"
     CDS             complement(23584..24870)
                     /gene="damX"
                     /locus_tag="LGK42_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBK2657513.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(24962..26050)
                     /gene="aroB"
                     /locus_tag="LGK42_001240"
     CDS             complement(24962..26050)
                     /gene="aroB"
                     /locus_tag="LGK42_001240"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK2657514.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(26107..26628)
                     /gene="aroK"
                     /locus_tag="LGK42_001241"
     CDS             complement(26107..26628)
                     /gene="aroK"
                     /locus_tag="LGK42_001241"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBK2657515.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(27029..28267)
                     /gene="hofQ"
                     /locus_tag="LGK42_001242"
     CDS             complement(27029..28267)
                     /gene="hofQ"
                     /locus_tag="LGK42_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBK2657516.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(28179..28583)
                     /gene="hofP"
                     /locus_tag="LGK42_001243"
     CDS             complement(28179..28583)
                     /gene="hofP"
                     /locus_tag="LGK42_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBK2657517.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(28573..29013)
                     /gene="hofO"
                     /locus_tag="LGK42_001244"
     CDS             complement(28573..29013)
                     /gene="hofO"
                     /locus_tag="LGK42_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBK2657518.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(28997..29536)
                     /gene="hofN"
                     /locus_tag="LGK42_001245"
     CDS             complement(28997..29536)
                     /gene="hofN"
                     /locus_tag="LGK42_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBK2657519.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(29536..30315)
                     /gene="hofM"
                     /locus_tag="LGK42_001246"
     CDS             complement(29536..30315)
                     /gene="hofM"
                     /locus_tag="LGK42_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBK2657520.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            30435..32987
                     /gene="mrcA"
                     /locus_tag="LGK42_001247"
     CDS             30435..32987
                     /gene="mrcA"
                     /locus_tag="LGK42_001247"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBK2657521.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVKIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(33155..33715)
                     /gene="nudE"
                     /locus_tag="LGK42_001248"
     CDS             complement(33155..33715)
                     /gene="nudE"
                     /locus_tag="LGK42_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBK2657522.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            34035..36170
                     /gene="igaA"
                     /locus_tag="LGK42_001249"
     CDS             34035..36170
                     /gene="igaA"
                     /locus_tag="LGK42_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBK2657523.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPFTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            36235..36903
                     /gene="yrfG"
                     /locus_tag="LGK42_001250"
     CDS             36235..36903
                     /gene="yrfG"
                     /locus_tag="LGK42_001250"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBK2657524.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            36914..37315
                     /gene="hslR"
                     /locus_tag="LGK42_001251"
     CDS             36914..37315
                     /gene="hslR"
                     /locus_tag="LGK42_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBK2657525.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            37340..38218
                     /gene="hslO"
                     /locus_tag="LGK42_001252"
     CDS             37340..38218
                     /gene="hslO"
                     /locus_tag="LGK42_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK2657526.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(38353..40077)
                     /locus_tag="LGK42_001253"
     CDS             complement(38353..40077)
                     /locus_tag="LGK42_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBK2657527.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMVIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
BASE COUNT         9544 a        10942 c        10201 g         9532 t
ORIGIN      
        1 tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat
       61 ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt
      121 ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta
      181 gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa
      241 atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca
      301 acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga
      361 aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca
      421 gccacagcct ggcgctgttc tcggcggcga tgttcattct cggggtggtc agcggcatca
      481 ccatgtcgat tggtacattc ctggtaacac aaatgtatga agggcgtcag cgcggttccc
      541 gcctgttatt taccgactcc ttcttcagta tggctgggat gattttccca atgatcgccg
      601 cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt
      661 atgtcgctat ttttattctg accttcggct gtgagttccc ggcgctgggc aaacatgcgc
      721 caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg
      781 ttgcggcgct gtgctacatc cttggtcagt taggttttat ctcctgggtg cctgagtatg
      841 ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt
      901 catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgta
      961 ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac
     1021 cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca
     1081 ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg
     1141 tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc ccgattgttg
     1201 aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg
     1261 tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc
     1321 attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct
     1381 acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg
     1441 tcagacaatt ccgtgactta agaaaattta tacaaatcag caatataccc cttaaggagt
     1501 atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg
     1561 gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg
     1621 gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg
     1681 atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcgtctt
     1741 acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac
     1801 acggcatcaa agttctggtc ggcgaacgtg ctatcaccat caaccgtcag gagaaggtga
     1861 ttcactccag cgccgggcgc accgtttttt atgacaagct gatcatggcg accggttcct
     1921 acccgtggat cccgccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca
     1981 ttgaagacct caacgccatt gaatcctgtg cccgtcgcag caaacgcggt gccgttgttg
     2041 gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc
     2101 acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc
     2161 agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacacccttg
     2221 agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac
     2281 tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc
     2341 agtgtggtct ggacgtagct ccgcgtgggg gtattgtcat taatgattcc tgccagactt
     2401 ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc
     2461 tggtagcacc aggctacaaa atggcgcagg tcgccgttga ccatattctc ggtagcgaaa
     2521 acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggcg
     2581 gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac ctcgacgaaa
     2641 gcaaagagat ctacaaacgc cttattgtca gcgaagacaa caaaaccctg ctcggtgcgg
     2701 tactggtggg cgataccagc gactacggca acctgctgca actggtgctg aatgctatcg
     2761 agctgccgga aaacccggat tctctgattc tgccggcgca ctcgggtagc ggtaagccgt
     2821 ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacca
     2881 aaggtgatct gattgctgcc atcaacaaag gctgccacac cgttgcggcg ctgaaagccg
     2941 aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg
     3001 cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt
     3061 cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc
     3121 tggcgaaaca cggcaaaggt tacggttgtg aagtttgtaa accgaccgtc ggttcgctgc
     3181 tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg caggattcaa
     3241 acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc
     3301 cgggcggtga aatcaccccg gaagggctga tggcagtagg tcgtatcgcg cgtgaattta
     3361 atctctacac caaaatcacc ggctctcagc gcctggcgat gtttggcgca cagaaagacg
     3421 atctgccgga gatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg
     3481 cgaaagcact acgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggcgttg
     3541 gcgacagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc
     3601 acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag
     3661 acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca
     3721 tgaaaccgcg tcatgcggat ctgctggcgg cggatatcga tcgcgaaacg ctgatcaaat
     3781 atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcac
     3841 cgtggttaga aaacctcgaa ggcggcattg attacctgaa agcggtgatc attgacgaca
     3901 aactggggct gaacgcacat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt
     3961 gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa cacttcatca
     4021 acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg
     4081 caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga accagtggaa
     4141 agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc tgttaggtga
     4201 cgagcaggta gcaattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat
     4261 tgatccgttc ttcgagtcca gcgtgctgtc acgcgggctg attgcggaac accagggcga
     4321 gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat
     4381 ggaagacgaa cagttttccg tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca
     4441 gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta
     4501 atgaccttcg gtatattgca ggacattttt taaacttttt gttttatttt tttgttttta
     4561 ttttttaaag gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaacgctgc
     4621 gcgcattgca cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc
     4681 gggcgcgtat gtgggtcttg ggatcatctt gattttcacg ctcggtaatt tgctcgaccc
     4741 gtccgtacgt ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat
     4801 cgccggttct gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg
     4861 cagcatcagc cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt
     4921 cggttccgtc ttcgttgcca tgctctatag ctggggtggc ggtagcctgc tgccggtaga
     4981 taccagcatc gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt
     5041 cttcaaaggt gcattgtgta actggctggt ttgtctggca atctggatgg cgctgcgcac
     5101 tgaaggggcg gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg
     5161 ctacgagcac tccatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag
     5221 cgaagcctac acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac
     5281 tttatcaggt gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg
     5341 tccggttgcg gacaaattta atcaaactga aacggctgcc ggttaattac taaggggttt
     5401 ttacgtggat catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg
     5461 cggtggtgat gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac
     5521 ggtgaatgca ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac
     5581 cctcgtcgaa gggccatttg atgaaagcct tctcgacacc tgctggctgg cgattgcagc
     5641 gacggatgat gacgcgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt
     5701 ctgtaacgtg gtcgatgcgc cgaaagctgc cagcttcatt atgccgtcga ttattgaccg
     5761 ctcaccgctg atggtggcgg tttcctctgg cggtacttct ccggttctgg cgcgcctgtt
     5821 gcgcgaaaaa ctcgaatcac tgctgccgct gcatctgggc caggtagcga aatacgccgg
     5881 gcagttacgc ggtcgggtaa aacaacagtt cgccacgatg ggtgaacgcc gccgcttctg
     5941 ggaaaaactg ttcgttaacg accgtctggc gcagtcgctg gcgaacaacg atcagaaagc
     6001 cattactgaa accaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt
     6061 gctggttggt gcaggtccgg gcgatgccgg gctgctgacg ctgaaaggac tgcaacaaat
     6121 tcagcaggca gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt
     6181 acgccgcgat gctgatcgcg ttttcgtcgg caaacgcgcg ggataccact gtgtaccgca
     6241 ggaagagatt aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct
     6301 gaaaggtggc gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa
     6361 cgcgggtatt ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta
     6421 ttcgggtatt ccgctcacgc atcgcgatta tgcccagagc gtgcgcttaa ttaccggaca
     6481 cttaaaaacc ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt
     6541 gttctatatg gggctgaatc aggctgcgac tattcagcaa aagctgattg aacacggtat
     6601 gcctggcgaa atgccggtag caattgtcga aaacggaaca gcagtcacgc agcgcgtgat
     6661 tgacggtacg ctcacacaac tgggcgaact ggcgcagcaa atgaacagtc catcgctaat
     6721 tattattggt cgggttgttg gcctgcgcga taaactgaac tggttctcca accattaatt
     6781 taatcagggc cagagaattc tggccttttt cgtttacaac tctctttata tattcagcag
     6841 ataaaaatat aactcattaa caaaaatcat ttgtttttgc cccgttatat tcaccgttac
     6901 agcgacttac aaaattaagt cattcactca ctgacgccga ttaaatattc ctgtatcgtt
     6961 cttaatgagc agtctactta tattcatata atcaatgaat attaatcaat atataataca
     7021 tagggaatgt aaatgaacaa atttattaaa gttgcactgg taggtgcggt actggctacg
     7081 ttaactgcat gtactggtca tattgaaaac cgtgataaga actgctctta cgactacctg
     7141 ctgcacccgg caatttctat ttctaaaatc attggcggtt gcggtcctac tgcacagtaa
     7201 gtctctgaca gaaatcggct aacaccggtt taattgcccg acgccctctg gattttccga
     7261 gggcgtattt gtttctatgt ccttcttgcc cccgcccgta aaaatatttt cactttaaat
     7321 tcaatttgat aaatacatcc attagccaca gcgcgatcct gtgcgaaatt ttgtgatctt
     7381 cctccacatt acataacatc atatgttgtt atattcatca tgcattgtca tgttaccttt
     7441 taaatgactg caaactctcc cctacaacgt attggacaag aaaaaggtat cgctatggga
     7501 agccaggaac tccaacgcaa gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca
     7561 accgtcggct ccggtatttt tgtatctgtg ggtgaagtgg caaaagcagc gggcacgccg
     7621 tggcttacgg tgctcgcgtt tgtcattggc gggttaattg tgatcccgca aatgtgcgtc
     7681 tatgcggaac tatccaccgc ttatccggaa aatggcgcag attatgttta tctgaaaaat
     7741 gccggaagcc gaccgctggc tttcctctcc ggctgggcca gcttctgggc caacgatgcg
     7801 ccgtcattgt cgattatggc gctggcgatt gtcagcaatc ttggcttttt aacgcctatc
     7861 gatccgttgc tcggtaaatt tatcgccgcc ggattaatta tcgcctttat gttgctacac
     7921 ctgcgctccg ttgaaggcgg cgcagcgttt cagacgctaa ttaccatcgc caaaattatc
     7981 ccgttcacta tcgtcattgg ccttgggatc ttctggttta aagcggagaa ttttgccgcc
     8041 cctaccacca ctgcgattgg cgcaacgggc agctttatgg cgctgctggc ggggatctct
     8101 gccaccagtt ggtcgtatac cggcatggcc tctatctgtt atatgaccgg cgaaattaaa
     8161 aaccccggaa aaaccatgcc acgagcgctg attggttcct gtctgctggt tctggtgctc
     8221 tacaccctgc tggcgctggt gatttccggc ctgatgccct tcgacaaact cgccaattct
     8281 gaaacgccga tttccgacgc cctgacctgg atccccgcac tcggcagcac cgctgggatc
     8341 tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg cgtgatgtac
     8401 cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tctttaaatg cttcggccat
     8461 gtgcatccga aatacaacac gccggatgtc tccatcatcc tgcaaggggc gctggggatc
     8521 ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct ggtgatgtgt
     8581 ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga cgattacaaa
     8641 ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc gtcaagcctc
     8701 attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg cgccgtcatc
     8761 gttattgcta ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg ccagctcaac
     8821 gctttgtcgt aatgttatct ggagaaaata aaatgttgga tattgataaa agcaccgtgg
     8881 actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc catgacgttc
     8941 cgctggtgca caccatcgtg gaagagatgg tgaagcgcga cattgatcgt atttatttcg
     9001 ttgcctgcgg atcgccactc aacgcggcgc aaacggcgaa acatctggcg gatcgctttt
     9061 ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc ccgtatcgcc
     9121 tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa gaggtaatca
     9181 aagcgctgga gctgggccgg gcctgcggcg cactcactgc ggcgttcacc aaacgcgcgg
     9241 acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac tgtatctggg
     9301 aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc ctcgcgccga
     9361 acgcggaaat cggcaagatc aaaaacgatc tcaagcaatt gccgaatgcg ctcggtcatc
     9421 tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc cagtggccga
     9481 tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa ggcattgtaa
     9541 cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag ttccgccatg
     9601 gcccgctgga gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc aatgatgaaa
     9661 gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac aacgtgatcg
     9721 tcatcgatta cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt
     9781 tcgtgccaat ggagtggctc tgctactacc tgtctattta caaagatcac aacccggatg
     9841 aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc ggcaacgggc
     9901 cgaatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg ccaccagcgg
     9961 ctgcctattg aacacgcatt tcgtgatgca agcgagctgg gttacgacgg catcgaaatt
    10021 tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat caaacaaatc
    10081 aaggcgctgg cgcagacgta tcagatgccg attatcggct atacgccaga aaccaacggc
    10141 tatccgtata acatgatgct gggcgatgaa catatgcgtc gcgaaagcct cgacatgatc
    10201 aagctggcga tggatatggc aaaagagatg aacgcgggtt atacgctgat ttccgcggcc
    10261 cacgcgggct atctcacgcc acctaatgtt atctggggac ggctggcaga gaacctaagc
    10321 gaactgtgtg agtacgcgga aaacatcggc atggatctaa tcctcgaacc gttaacgccg
    10381 tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc gctggtgcct
    10441 tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg
    10501 atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt cgacagcgac
    10561 ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgag ggaactgatg
    10621 cgcgatatta tcgaccgggg ctatgagggt tactgtacgg tggagctggt gacgatgtat
    10681 atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc gctgctgccg
    10741 gaggatgaga gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat atctacccgc
    10801 aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac tgcactcgct
    10861 acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc acaaagctga
    10921 agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa cacggcgtta
    10981 ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac tacaccgaag
    11041 gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg cagtatgaca
    11101 ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt cccacagctg cacgctgcgg
    11161 gcaaacttac cgctttcgac ttctccgaca agtgggacag ctcgctctgg cagacactgg
    11221 tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaagcg ctgcgtctga
    11281 agatgaaagc gattgttgcc cgtggcgcag gcgtggtgat tgtcacgctg ggtgaaaacg
    11341 gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg gtgacggtta
    11401 tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc tggtctgcgg
    11461 ggatgacatt accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa accattcagt
    11521 accacggtgc ctggtaggta taacgttggc gtgagcatct tcacgccaac gtgctgttac
    11581 ttgccggaaa acgaccctat aatccgagta attcattctt tatttcaggg tcgattatgt
    11641 cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag cggttgctgg
    11701 atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa aacgagcttt
    11761 gtacgcaata taacgtcagc cgcattacta ttcgcaaagc tatcagcgac ttagtggcag
    11821 acggcgtact gatccgctgg cagggcaaag gcacctttgt acaaagccag aaggtcgaaa
    11881 acgccctgct gaccgtcagt ggttttaccg attttggcgt ctcacaaggc aaggcgacga
    11941 aagagaaagt gatcgagcag gaacgagtca gcgccgcgcc gttttgcgaa aagctgaaca
    12001 tccctggcaa cagcgaagtg ttccatcttt gccgggtgat gtatctcgat aaagagccgc
    12061 tgtttattga tagttcctgg atcccactgt cgcgttatcc tgactttgat gagatttacg
    12121 ttgaaggaag ctccacttat cagttatttc aggagcgttt cgacacgcga gtggtcagcg
    12181 acaaaaagac catcgatatc tttgccgcca cccgcccgca ggcaaaatgg ctgaaatgcg
    12241 aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat gacaaaccgg
    12301 tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaacgatt gataataaaa
    12361 gacattaacc gtaggccgga taagatgcgc cagcatcgca tccggcgatg ctggcgcgtt
    12421 gaattttaca tcccgtacgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
    12481 cgttcagtac ggaatttagc ttgacggtgc tatccagcat taaatactgg caatactctc
    12541 acgcaaaatc cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca
    12601 atgttctgat tgtggattcg cctggcgaaa cgttccggaa aggcgataaa agagcggcgg
    12661 gatttcatat ttcgactctg cacccaccgg gtaaggcaag gcaccgcgct tttgcgcctc
    12721 ttccagcact ctggcggcaa tcggctgatg aaactcgaca atcaacacct tcgactgcgc
    12781 attagcaatc accgcgcttt tcacttccgg caccgcacca ccgttaagca aagccagcaa
    12841 ccgttcagat acccctgcct gcaccgcgtg catcactggc gcaaacacca gaccacgcaa
    12901 tacttccagc gcctgtgcgc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat
    12961 acggttgata acatcagcat cgccgaccac tgcaccaaca ccctctggcc caaatagctt
    13021 gaagcaggaa aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ccaccttcat
    13081 caccgcatag ttgtcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac
    13141 atctgccagc acgtagctgt cctgcggctg ctggcgcgta tgctgcacca gcgccgcatc
    13201 cggttgttgc tcgtcgacga cctgcttcag tgccgacagg tcattgaaat caacagtaat
    13261 aagcgtcagc cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg
    13321 caccagaaga cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt
    13381 ccccgcgccc tgcaccagcg ccgcagcctg tgcgtgaaat gcatcagcca gcacctgttc
    13441 cacacgctgg gtaacgcgcg gttgattcag tcctggcgtt aagcctaaat caccgccagt
    13501 aagaaactcg ctgccgggga aatggcgaca aatgctatcc accagcgcaa actgcttttg
    13561 ctgcgcctca ataatcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa
    13621 gccggaacga acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact
    13681 gccacgaccg gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa
    13741 atggcaaccg cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa
    13801 ccgaccagca gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca
    13861 ttacgcaccg acggatagcg ccccagccat ttgccgatcg aacgaagcaa taaaacttct
    13921 gccgaaataa ccactgcacc taataccgct gcaaccatcg gattcggcga gagatagccc
    13981 accgcataaa caaaggtaaa gcccgcaact gcatacacac ccgttgctaa cgcggtggtg
    14041 gcaatcaacg gcacaaaccc cagtccgcgc ataaattctg ccagagccgc ctgattaatc
    14101 agcgtttgcg attgttccgg cgttacgcct gcggaatatg ctttctccag tgtgaagatc
    14161 gacacttcac tgccagcaaa aatcttcatg ctggcaacgg cggcaatcaa tgctcccacg
    14221 atggcgatat agggtaagtt tttgataatc cgtgacgtgc gttcttcaaa caccgaaagc
    14281 ccgctggcat caatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc
    14341 agcagcatca ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag
    14401 cgcacgacta ccacacgggt catcagtacc accacggcgg cgatcagact ttgcttccag
    14461 ccaaactggt agaaaatcgc caccagcggg aacagtgcaa aagctgaaac caccggcgag
    14521 cttaattccc ccaggctacc taatacatcc accggcagcg cggtcagcag ctggtttact
    14581 ggcaacaggc aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg
    14641 ctgtttatcg ccaggacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc
    14701 agcccggttt tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca
    14761 aaagcgatac tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc
    14821 gggcggatgc cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc
    14881 gtcaggcacg ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca
    14941 gatggttaat cagcatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca
    15001 ctttcccttc ggcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg
    15061 caatggtgca acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg
    15121 caccggtatt acacgcaccg atgtagtaat ccagttgccc ggatttcact ttcattgccg
    15181 cttccatgtc gttgtgaatg aaaacttcaa aactgccagg agccgtcgct tcaatggttt
    15241 ttttaatctg ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat
    15301 tggaaaaatt gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct
    15361 gactggcgca attgcggaat aaaggtggtc aataaatagt catagccata accaccattg
    15421 gcttttaaat gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca
    15481 cggtcacgta gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta
    15541 ccaatggtgt cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgaga
    15601 ttgtctttca gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc
    15661 aacagagcca gttgttccag ccccatggtg ctgaacgaag tatgcgtgga gatcgggcgt
    15721 ccggtctggt tatgcgccag cgcagcggca ataaacacct tctcttccag cggcgtaatc
    15781 ttcccttcgc tagagccgat ctccgcgata atcccggctt tcaggtcggt gccatcgata
    15841 ccctgttcaa tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcgccaca
    15901 tgctccggga aaaaagcgtc ctggtaataa ccggtgcagg ccaccacgtt gatccccgtc
    15961 tcgcgcatta catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca
    16021 atcacattac gcacgccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac
    16081 tgatcaaggc ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca
    16141 tgggccaggg tgtaacccgt cggatcaata ctcatagggt gtctcctgca aaccggaggg
    16201 aggaaagaaa agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag
    16261 cccccacatc agaaagcgtg gtgcgtacac cgagttgcgt gtggaccagc ccttgctgat
    16321 aaaccagcac tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac
    16381 cgtgatccgc catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa
    16441 ggttacggtc aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag
    16501 cgaggtcggt ttcctggata ttgatgcaaa taaacgccgt cggatgcgtg ttaaattcgt
    16561 cgagggtgat atccataatc cgctggctat ccaccagatt ttgccagctc acgccataag
    16621 gattgttgac gatatctgcc accttaccca ccagcacggt tggcacgcct gcttcataca
    16681 gtttttgtgg cacctgcact ttttcatcga cgccatagcc catatgcacg acctggaaac
    16741 cgttgtcata agcgccagaa cgcggcgcat tgataccaat aaagcgccct tctttgcttt
    16801 ctgcggcatc gagaatgcgt tgactgtcgg ttaacaggcc accaaatgta atgacccgac
    16861 cgacctgtac ctgctcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca
    16921 cagagagatt ggcggtaatg ttatagacct ggcctaaatc cgcctcgaga ttatcgccaa
    16981 tcgcaaccgc ctgattgacc cacagaaatt gcagttcatc gccacggcgc tccacctgcc
    17041 aaccggcgga tactaatgcc tgctcaacac ggtcaatcac atcgcaaaaa ggcatccgca
    17101 gcggcggtaa cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt
    17161 catgttgcag ctctgccacg ccccaggttg cggaatctga cggctgcata tcgcctggcg
    17221 cataacccaa tgcgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca
    17281 actggctcag gatgtgacca caggtattcg ctcccgcatc ttgcggacgc accagcgtga
    17341 catctttcat tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccatta
    17401 cgcccccaga gagtttccaa gactgtcata acgaccaacg attttcggtt cgccacggtg
    17461 aataccggac accagcacca catcgctacg ggtgacaaaa atctgcgtgc ggaaacagag
    17521 caccactgca ctgctcaccg gatactcgcc tgccagcggc agggtgtagt cgatactgct
    17581 gtcatccaca gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag
    17641 cgcatgttgc gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg
    17701 gaaatgatgg gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg
    17761 ctggtttgcc ggaatagtgc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta
    17821 ttgcgccagc aatggcagcg aagtgcagct ggtcgctgaa ggcgcgttca gttgctcaat
    17881 cgcaatacca gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt
    17941 cggcaaaact ttcccggcag cctcatccca aagcaggcaa gggaaatggg ttagtcccgc
    18001 caaatgcagc cccggcagtt tcttaatttc agcaaccacc tcgtgcagcg aatgctgtac
    18061 aaaaccgctc tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac
    18121 agactggatt cgcccggcct tcaccgccgc cgcagaaact tcccgcgctt tgtcgagagt
    18181 aaacacggtg atgacgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat
    18241 ttgtaccaga tgcccctgat gcgccaccgg caaaccagcg cggcgcatga ctcgcgcctc
    18301 tttgtaatcc accgccacaa tgccgctgta gcccaacgcc agcagttttt ccgccagcca
    18361 tgggttacga ccgaattgtt tggtcatcag atacagttca atgccgtaaa gccgcgccgt
    18421 ctcaatcagc cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg
    18481 ggcgattttg ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg
    18541 tttcaatgct tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat
    18601 ttttttgaat atttaggttg tgcgacgtgg atttgtcagg agcttcaaac ctcttcgtta
    18661 gcagccatcc ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact
    18721 tccagtgcga attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg
    18781 gattgcgcca gctctgccag caattcgtta tccagcggct ctatttcttc accacggcga
    18841 ctgcgcatca gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc
    18901 ggcagatggc actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc
    18961 ttatcaataa cgcctgcctc gcaaagcagg ttgagtctgg tttccatatc agatgccttt
    19021 cgtttaataa ctcggttcct gttccccact cactacccgt tgttgatggg cgagcagggc
    19081 gtgtttatct acaacggcac gcagcagttg ttgcattggg taatcatcga ctcgcgtcag
    19141 gaccacggct tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcctctaa
    19201 tcccagcatg gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc
    19261 tttaacaatg cgttgtaaac tctcgtgata agagagatcg actcgttcca catcactacc
    19321 gccaaaaaaa acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg
    19381 cttcacgttt gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg
    19441 cggccccaac tccagcgcga ggcataaccc tttttgcgtg agataacttt ccgccgccag
    19501 tcgggaaacc accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc
    19561 gccgcgcata tgcgcatagt aaaaaggaat gccatcaaac tgagctttca gcccgctcgc
    19621 taaaccttcg tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc
    19681 cacatgactc agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg
    19741 gcgttcaatc cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc
    19801 aaccgatgaa cggcattcag ttgccagctc atctatggtt ttcaacctat taccgcattt
    19861 ttcacccaac aaataacggg ccagcgtcgt gataacgacg ccttcttttt tgataaacgt
    19921 tcgacgcata atatattttc aataaaatga atgtttatat cttcattaaa atgaagataa
    19981 gtggcaactg tgagatgtgg aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa
    20041 tgaggataaa aaaaccgggt ttccccggtt tcagagtgat gacgaagtga tttattcgac
    20101 aatgcgagca aagggtaatt cccactggcg cttgcctgct cccctcaccc taaccctctc
    20161 cccaaagggg cgaggggacc gtccgagcgt tttgatatta cggcttcgcc acaaaaccaa
    20221 tcgcttcgta caccgctttc agcgtacggg aagcgtgtac actggctttt tctgcgccgt
    20281 ctttcatcac ctgttgcagg aaggcttcat cgttgcggaa acgatgatag cgttcctgca
    20341 attcagtcag cataccggaa acggcatcag ccacttcacc tttcagatga ccatacatct
    20401 tgccttcgaa ctgtttttcc agttctggga tgctctggcc cgttaccgca gaaaggatat
    20461 ccagcaggtt ggaaacgccc gctttgttct gcacatcgta gcgaactacc ggcggctcgt
    20521 cggagtcagt gaccgcgcgt ttgattttct tcactaccga tttcggatct tccagcaggc
    20581 cgataacgtt attgcggtta tcgtcagact tggacatctt cttggtcggc tccagcagcg
    20641 acattacgcg cgcgccagat ttcggaataa acggctccgg caccttaaag atctcgccat
    20701 acagcgcgtt gaaacgctgg gcgatatcgc gactcagttc caggtgctgt ttctggtctt
    20761 cacccaccgg taccagatta gtttgataca gcaggatgtc cgcagccatc agcaccggat
    20821 agtcaaacag accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactggg
    20881 tcatgcggct cagttcgccg aagtaggtat agcagttcag tgcccagcct aactgcgcat
    20941 gttctggtac gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacaagcca
    21001 gatacaaggc cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg
    21061 tgatcgcgtg ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc
    21121 actgacgcag cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac
    21181 taaaaacgat gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat
    21241 gcgggcagaa ggtcatttat agactgataa attacatcag gctggctgag atcgatagcc
    21301 tcgccgtagt tatatccgta ggttaagcca acggatgggc aacctgccgc ttttgccgcc
    21361 tgaatatcat tgcgtgagtc gccgacaaac agcatctgtt gtggggcaat tcccatccgc
    21421 tcagccacca gtaacagcgg gtccggatga ggttttttgt tttgcacatc atcgccgccg
    21481 ataaccacgc taaagtattt ggcaatatct aaggcttcga gcagcggcgc gacgaacggc
    21541 gtcggtttgt tggtgaccag gcctagcggc aggcctttag cctgcaacgc gcccaacgta
    21601 tcggcagcgt gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata gtagcgatcg
    21661 aacagtttac gcagaatacg tacctgttct tctgccggaa tgtcgtcatc aacgggcggt
    21721 ttacccattg ttttacgctg agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc
    21781 atcagaacat ctgcgccgtt accaatccag gtaataacgc gttcttcacc tgcgacgggc
    21841 aactccagcg catacagcgc catatctacc gcagcagcaa gaccgggagc actgtcgacc
    21901 agcgtaccat caagatcaaa agcgacgccg cgaatatctt caaacttatt catgacttac
    21961 ctttgccagt tcactgcgca tttcatcaat gacttttttg tagtctggct ggtcgaagat
    22021 tgccgaaccg gcgacgaaca tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac
    22081 cttcacgcca ccgtctactt ccagacgaat gtcaaagcca gactcgtcga tacgacgacg
    22141 tacttcgcgc agtttatcca gtgtttgagg aatgaacgac tgaccgccga aaccaggatt
    22201 gacggacatc agcaggatca catccagctt atccatcacg taatccagat agctcagcgg
    22261 tgtcgccggg ttaaatacca gtcccgcttt acagccattt tctttaatca gttgcagcgt
    22321 gcggtcaaca tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc
    22381 gaaatcaggc acaatgcgat cgacaggttt caccatcagg tgtacgtcga taggggcggt
    22441 aatgccgtag ttacgcaagg atttcagcac cattggccca atcgtcagat tgggaacata
    22501 gtggttatcc atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt
    22561 atcttcaccc aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt
    22621 catccgcttc tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa
    22681 gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact
    22741 ttgacgacat gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg
    22801 gagatcagca ctggaatatg gcgctcaacc agaccttcgg cgatctccgc cagatgcgct
    22861 tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc
    22921 ggtgcataag gcggatcgca atagacgacg gatgcatcat ctgcgcgcgc catgctatcg
    22981 gcgtaagact cacaatagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct
    23041 gcttccggga aatagggttt tttgtagcgg ccaaacggca cgttaaactc accgcgcaga
    23101 ttgtaacgac acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga
    23161 cggaacggat cctggctttt gttgaactct tcgcggaact gatagtagac ctcggcgcaa
    23221 tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc
    23281 acaatgttat agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag
    23341 tcggtgttga gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg
    23401 ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa
    23461 gcgcgatttt tcttcatgct gactaactaa ttacaccttc tccggctgtg gagaaagctc
    23521 cgacagcatc cagcgcttca ggcaactccc gcaacgataa cccattgcgg gagataacct
    23581 tgattacttc agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg
    23641 aacatctgct ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc
    23701 agaaaccagg acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt
    23761 cagattctct ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg
    23821 cagagtgtaa tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc
    23881 tccagcggct ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg gagccgtagt
    23941 cgccgtctct tttggtgctg gcgcagaagt cgcagccggt gccttcgtgc tcgccacagg
    24001 agcagctggt tcagtacgct tcggcgtctg tgctaccggc ttcggttccg ttttcacggt
    24061 tgcttgcggt tttttcggtt caatcaccgc ctgctgacga gctggacgcg tagtggccgg
    24121 acgttcagca gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gtactggcgc
    24181 gacagttgct ggttcagtcg gcaatgtaga attgaccgcc acattgttca actgttgctg
    24241 attttgtggc tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc
    24301 atccgttgcc gccggggttt gcccttgagt cggcgtagaa gagatcggtg gcagagaaac
    24361 atcctgctga gtattttccg cagacgtggt tcccggcgct ggctgcaccc cattcgcctg
    24421 atcggtcgca ttgcctgcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga
    24481 agaggtcgag ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag
    24541 aatgccgacg cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg
    24601 cttacgcgga cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat
    24661 ttcttcttcg atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc
    24721 gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg
    24781 ctcagaacgt tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt
    24841 cagctcgtct tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac
    24901 cacgttgcaa ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt
    24961 gttacgctga ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc
    25021 gaacttcact cttaccaatt gccaacggaa gaattaagcg tatctctccc gcaaggactt
    25081 tcttgtcacg cagcatatgc ggtaaatacg cctgcgcgga catttcgcgc ggcccattga
    25141 ccggtaaccc agcccgcttg agcagggtta taatacgctg cgtttcggca gaactaaact
    25201 gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat
    25261 gtaaccaatt gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat
    25321 tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt
    25381 cacaacaacg gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca
    25441 gattctcttc cagccagtta aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt
    25501 ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca
    25561 ccactgaagc aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg
    25621 cagttttgcc gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga
    25681 aacgaacacc gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc
    25741 cgccaagcgc caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga
    25801 cggtatcgag tacagccagg cttttatact gctcgccgtc agggaggata acgctatcga
    25861 cgttaacacc cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg
    25921 tttcgttggt gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt
    25981 cattaaacaa accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa
    26041 tcctctccat aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag
    26101 ccagaattag ttgctttcca gcatgtgaat aatctggttt gcaaccactt tagcgctttg
    26161 atcatcagta cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc
    26221 caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc
    26281 acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg
    26341 agcggaaaga cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac
    26401 aataccctgt ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc
    26461 ttcgccttct aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg
    26521 atcggaatcg taaaattcca tattgagttg ttgagctaac tggcgcccaa tagtgctttt
    26581 tccggcaccc ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact
    26641 actaagacta ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac
    26701 tgaaacctca taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt
    26761 ttggcaactg aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcgctgcct
    26821 tgctaccacc gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa
    26881 tgccgcctga atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca
    26941 aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc
    27001 ataaaaacat taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa
    27061 acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc
    27121 agggaatgtc gccaagcaac ggtacgctat cctgacccga tttattttta cgggtaaaaa
    27181 tgccgcccag cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg atctcctgct
    27241 tatcaatcgc cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc
    27301 tgatgtgtaa tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc
    27361 ccaggacggc ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaaacctgat
    27421 atggaatttc gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg
    27481 gactggcgat aatatccagc tgctgttttt gttcgagcgc ggaaagctca agatccagca
    27541 agcgtccgtt gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga
    27601 ggtcgctacc aagcgtggtg acttgcccaa caccaccagc gtgttgcgca tcggccagcg
    27661 tccatttcac gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca
    27721 gctcaacctg cccgaccgga agatccattt gcgctaccca ctgttcaagc gcgcttaacg
    27781 ccgttttgtt atctcgtagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt
    27841 tggcactcag tagcttctcc cccgctttcg ccagttctcc cgcgtcggcg tattgcaggg
    27901 ttatactgcg attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctggc
    27961 gggcgatatt gtcattctgc caggcaatag aatgcactga gagaatgttg ccctcctggc
    28021 gcgttatcag cccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg
    28081 ttagatgtaa cgacaccgta ccgctgacgt ctggcgacac gaccaggttc aacttctcct
    28141 gttcagccag cgcctgcaac acctgagcca ccggaacgtc atccaccatc agcgtcactt
    28201 tttgcggctt tgccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt
    28261 gcttcatttg tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg
    28321 gtacccagcg ttagtacgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca
    28381 tcgttttgct gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgcgc
    28441 tcgcctcgcc ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcataga
    28501 tcttccggcg gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc
    28561 aacaaccagc gtttaaccct catgcggcgt ctccagttgt agcgtgaaca aaagatcatc
    28621 accttccacg cttaacgaaa aacggctcac gctgacgttg cgctctgcca gccgtgtaaa
    28681 tgccgacggc actgcttccc acagcgtttt caacgccaac tcgcctccct gcgcggatgg
    28741 atgccaggaa accagttgcg cgccggataa ctgaaaatcc agtggcgaaa agggcagcgt
    28801 tttttcctcg ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg
    28861 atgacttgcc cgtagtcgaa ttaatgcgtc gcgctcttca tggtgtgtcg atgatagaaa
    28921 aatgagcgta actaacatca gcaaccagaa tgcccagcaa agctggcgga ggcggggtga
    28981 tgtggcgaac caccagtcaa agaacatgtt catcgctaac cttccttgtt aactgatact
    29041 caaattgcca gcgtccctgc gcatcctgct gcgtggctcc ccgctgattg agatgaaaag
    29101 acgcatcctg gcggagtgac gtttctagtg cgtttaacgc ggtaatgctt gttgtcagcc
    29161 ccttgatctc cagcgttccc tgctgccagc ttatcgttgt cagccaggcg tgctcgggta
    29221 aaagcgccgc cagtgcttcc agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct
    29281 gccgttgcga acgctgttcg cgtaactgct gttgctccag caaacgtggc ttcgttatct
    29341 gtaagctgcg ggcgagttgt tgttccgcct gaagcaaaac tgcgtctatg cgcgcttcgg
    29401 cgctgcctgt cagacgcagt attagcgtta tcccgacggc cagcagcaga ggcgcaacga
    29461 acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat
    29521 taattggcgg gttcatcagt acgccttccc aagcgccagc cccagcgcga tggcaaagtc
    29581 tccacctggc ggcggtagcg gcggctgacg aacagaaacg gcctcccagg gatcaaatcc
    29641 cccttcgcca catatcgcga cgctttcggg atcaacggat aacgctgccg ccagctcctt
    29701 cgcgctagtc atccccaccg ccagtttgcg cccccagcga cagcgtgtcg cccacagcca
    29761 ctgttcgtta tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaattg
    29821 ctgtaatgca ctggcatccg gggtgatcgc gctcacatga acacgcaacc tttctgccag
    29881 agtaagtagc gttgccagct ctttgctttg cgcggcagtc acgttatagg cggggctgag
    29941 tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgggccat
    30001 cgtccctgac agccaggccg tttgctcccg ctcaccaagg gacatcgacg ggcgcggaaa
    30061 tgaccgctgt aatgtgcgac tggcgggaaa cgccaacata atgtgatgac gctgcggcag
    30121 ttcgcgactc cacggtaaca acgttttagc cagctgctgc gcatcaacaa tccgcccatc
    30181 tttgataatg tcgttctcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgc
    30241 tccccgcacg atcgcaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca
    30301 gatcttaaat gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat
    30361 gcgtctggct tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa
    30421 atgggaaatt tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc
    30481 tgctgggagc aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg
    30541 tggcgacatt aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg
    30601 agctgattgc tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccac
    30661 cggagatggt gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg
    30721 ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac
    30781 aaggggcgag taccattacc cagcagctgg cgagaaattt cttcctcagt ccagaacgca
    30841 cgctgatgcg taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga
    30901 aagacgagat cctcgagctt tatctgaaca agatttacct cggttaccgc gcctatggtg
    30961 tcggtgctgc ggcacaagtc tatttcggta aaacggtcga ccaactgacg ctgaacgaaa
    31021 tggctgtgat cgccgggctg ccgaaagcgc cttccacctt taatccgctc tactcaatgg
    31081 atcgtgccgt cgcgcggcgt aacgtcgtac tgtcgcggat gctggatgaa gggtatatca
    31141 cccaacaaca gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg
    31201 agattgcttt ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt
    31261 atggcgaaag tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc
    31321 agcaggccgc acagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct
    31381 atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga
    31441 ttaccgatac gctgaaggcg ctgccaacct atggtccgct gctgcctgcc gcagtcacca
    31501 gcgccaatcc tcagcaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta
    31561 tggaaggcgt tcgctgggcg cgtccttacc gttcggatac tcagcaagga ccgacgccgc
    31621 gtaaagtgac cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg
    31681 catggtggct ggcacaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa
    31741 acggtgccgt tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg
    31801 ccacccaggc actgcgtcag gtgggttcca acatcaaacc gttcctctac accgcggcga
    31861 tggataaagg tctgacgctg gcaagtatgt tgaacgatgt gccaatttct cgctgggatg
    31921 caggtgccgg ttctgactgg cagccgaaga actcaccacc gcagtatgct ggtccaattc
    31981 gcttacgtca ggggctgggt cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga
    32041 tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg
    32101 tccacaccga atcgctggcg ctgggttcag cgtccttcac cccaatgcag gtggcgcgcg
    32161 gctacgcggt catggcgaac ggtggcttcc tggtggaccc gtggtttatc agcaaaattg
    32221 aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg
    32281 atattccagt gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg
    32341 aagatgtcgc tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc
    32401 aggcaaatca ggcgttagtg gcgaagactg gcgcgcagga gtacgcaccg cacgtcatca
    32461 acactccgct ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgagccag
    32521 gctggcaggg taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga
    32581 aaaccggtac cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggcgttg
    32641 tgacctcggt ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt
    32701 ccggagcgat taaagatcag atatcaggtt acgaaggcgg tgccaagagc gcccagcctg
    32761 catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc
    32821 cgccaggtat tgtgacggtg aaaatcgatc gcagcaccgg gcagttagct aatggtggca
    32881 acagccgcga agagtatttc atcgaaggta cgcagccgac acaacaagcg gtgcacgagg
    32941 tgggtacgac cattatcgat aatggcgaag cacaggaatt gttctgatta aaaaggcgct
    33001 tcggcgcctt tttcagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa
    33061 cactttaccc gtctagcaac atctagaaaa atcaatatat tgcagaaact tcgtaagcct
    33121 gataagcatt atgcatcagg cgaactttac gcatttacac tcgcccctgc cctttcaacc
    33181 attcacgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    33241 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    33301 cttccagcga ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaaat
    33361 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatccaacct
    33421 cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca attaaacctt
    33481 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    33541 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    33601 aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta aacagtcggg
    33661 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg
    33721 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    33781 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    33841 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    33901 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    33961 agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga cagactaagt
    34021 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    34081 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    34141 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    34201 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct agcgcggcac
    34261 cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca
    34321 cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    34381 aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc gttgaactga
    34441 tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca
    34501 tgcaggaaac gcgcggctat gccttgcaac ctgttccgtt cacgcaggcg tcgattcgcg
    34561 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat gaagaatatg
    34621 cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt
    34681 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcattac
    34741 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcgag
    34801 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    34861 aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca cactggcagc
    34921 catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc gaccgccacg
    34981 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    35041 actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt atgctgttat
    35101 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    35161 ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat
    35221 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa
    35281 ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac gcccgctcat
    35341 taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa gcagttaatc
    35401 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga
    35461 ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg aagacagcgg
    35521 atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    35581 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc gatggcgtga
    35641 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    35701 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    35761 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    35821 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    35881 tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc actgacgcca
    35941 atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    36001 tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat ggcgtacagg
    36061 cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc tattatgaaa
    36121 gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    36181 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    36241 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacattgctc
    36301 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    36361 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    36421 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    36481 gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat tccgtttctt
    36541 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca tccgcacaac
    36601 ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt actttccacc
    36661 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    36721 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    36781 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg gattgccgag
    36841 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    36901 tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg
    36961 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt
    37021 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    37081 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    37141 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    37201 aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    37261 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    37321 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    37381 ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    37441 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    37501 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag gcgacggtcc
    37561 gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg tggcgcgcgt
    37621 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    37681 gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc tggaaggtga
    37741 taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct
    37801 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    37861 aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    37921 catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    37981 cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    38041 tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata gcatcctggc
    38101 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    38161 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    38221 agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg atgtgctgcg
    38281 cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc gtcgtatccg
    38341 ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc
    38401 gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga gtaatttttc
    38461 tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt ttctgtctga
    38521 gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg
    38581 accatcacta ttcagatctt gctcgaccag gacgcaggcg tctttttcaa tacaggtcat
    38641 cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taacagaacc
    38701 aggggcaatt aacacatttt cggctaatac tttttccgat acctgttcct gaagatgttg
    38761 ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca taaacccggc
    38821 atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    38881 gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac
    38941 gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca ccaacaccaa
    39001 taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc cattacgcca
    39061 gacgatactg acaaaatacc ccagcgacca caccagtaac accagcaccg ccagcgcgcc
    39121 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg cccaggcggc
    39181 gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac gcaacggccc
    39241 cgtccaggga agtgacgctt tttgcggatc gcggacaata accattaaga tcaattgcaa
    39301 aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc
    39361 cgtaaacggc agagtgatga taaacatcag ggttagcaat gatactaacg gcagcaaccc
    39421 cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgtgcgcgc
    39481 gagaatcacc gccagtgcgg taaccagacc taatgtaaga tacataaacc agtcggttgc
    39541 aaaaaagagc gtattgaaaa acttgatgcc aacaagttta aacaactcac cccacagtaa
    39601 aagcaccagc cacgtcaagc cattggcgag aaaaatcacc agtaatataa gtacgttatg
    39661 ccatactgac tggtaaaaat agcgatagcg gctactgtca ttgcggatgc gaaggctttg
    39721 ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa aatcccaaat
    39781 cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcaacc agccgctcat
    39841 ccccagcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct gtttaaaaga
    39901 gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc gcaggcacgc cataaacaat
    39961 ccaactgtga ttttttccgg caagccaggc gatcaacagg tagcaaacca gcccctgaag
    40021 taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt
    40081 ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt
    40141 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccga ttgaatttgc
    40201 atcaatttca ttaaggaat
//
LOCUS       DAFAVF010000024        40084 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFAVF010000024 DAFAVF010000000
VERSION     DAFAVF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.024"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            179..2377
                     /gene="speF"
                     /locus_tag="LGK42_001254"
     CDS             179..2377
                     /gene="speF"
                     /locus_tag="LGK42_001254"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBK2657528.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2374..3693
                     /gene="potE"
                     /locus_tag="LGK42_001255"
     CDS             2374..3693
                     /gene="potE"
                     /locus_tag="LGK42_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBK2657529.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3749..5389)
                     /gene="pgm"
                     /locus_tag="LGK42_001256"
     CDS             complement(3749..5389)
                     /gene="pgm"
                     /locus_tag="LGK42_001256"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBK2657530.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5415..5960)
                     /gene="seqA"
                     /locus_tag="LGK42_001257"
     CDS             complement(5415..5960)
                     /gene="seqA"
                     /locus_tag="LGK42_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBK2657531.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6145..6909
                     /gene="ybfF"
                     /locus_tag="LGK42_001258"
     CDS             6145..6909
                     /gene="ybfF"
                     /locus_tag="LGK42_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2657532.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7049..7342
                     /gene="ybfE"
                     /locus_tag="LGK42_001259"
     CDS             7049..7342
                     /gene="ybfE"
                     /locus_tag="LGK42_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBK2657533.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7482..8012
                     /gene="fldA"
                     /locus_tag="LGK42_001260"
     CDS             7482..8012
                     /gene="fldA"
                     /locus_tag="LGK42_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBK2657534.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8222..8308
                     /gene="uof"
                     /locus_tag="LGK42_001261"
     CDS             8222..8308
                     /gene="uof"
                     /locus_tag="LGK42_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBK2657535.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8301..8747
                     /gene="fur"
                     /locus_tag="LGK42_001262"
     CDS             8301..8747
                     /gene="fur"
                     /locus_tag="LGK42_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBK2657536.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8831..9157)
                     /locus_tag="LGK42_001263"
     CDS             complement(8831..9157)
                     /locus_tag="LGK42_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657537.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9207..10613)
                     /gene="chiP"
                     /locus_tag="LGK42_001264"
     CDS             complement(9207..10613)
                     /gene="chiP"
                     /locus_tag="LGK42_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBK2657538.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11059..12723)
                     /gene="glnS"
                     /locus_tag="LGK42_001265"
     CDS             complement(11059..12723)
                     /gene="glnS"
                     /locus_tag="LGK42_001265"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBK2657539.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12926..14872)
                     /gene="nagE"
                     /locus_tag="LGK42_001266"
     CDS             complement(12926..14872)
                     /gene="nagE"
                     /locus_tag="LGK42_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBK2657540.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAILGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15205..16005
                     /gene="nagB"
                     /locus_tag="LGK42_001267"
     CDS             15205..16005
                     /gene="nagB"
                     /locus_tag="LGK42_001267"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK2657541.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16065..17213
                     /gene="nagA"
                     /locus_tag="LGK42_001268"
     CDS             16065..17213
                     /gene="nagA"
                     /locus_tag="LGK42_001268"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK2657542.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17222..18442
                     /gene="nagC"
                     /locus_tag="LGK42_001269"
                     /gene_synonym="nagR"
     CDS             17222..18442
                     /gene="nagC"
                     /locus_tag="LGK42_001269"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBK2657543.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18490..19242
                     /gene="nagD"
                     /locus_tag="LGK42_001270"
     CDS             18490..19242
                     /gene="nagD"
                     /locus_tag="LGK42_001270"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBK2657544.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19499..21163
                     /gene="asnB"
                     /locus_tag="LGK42_001271"
     CDS             19499..21163
                     /gene="asnB"
                     /locus_tag="LGK42_001271"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBK2657545.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21544..21620
                     /locus_tag="LGK42_001272"
     tRNA            21544..21620
                     /locus_tag="LGK42_001272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21578..21580,aa:Met,seq:cat)
     gene            21629..21713
                     /locus_tag="LGK42_001273"
     tRNA            21629..21713
                     /locus_tag="LGK42_001273"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21663..21665,aa:Leu,seq:tag)
     gene            21737..21811
                     /locus_tag="LGK42_001274"
     tRNA            21737..21811
                     /locus_tag="LGK42_001274"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21769..21771,aa:Gln,seq:ttg)
     gene            21846..21920
                     /locus_tag="LGK42_001275"
     tRNA            21846..21920
                     /locus_tag="LGK42_001275"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21878..21880,aa:Gln,seq:ttg)
     gene            21936..22012
                     /locus_tag="LGK42_001276"
     tRNA            21936..22012
                     /locus_tag="LGK42_001276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21970..21972,aa:Met,seq:cat)
     gene            22061..22135
                     /locus_tag="LGK42_001277"
     tRNA            22061..22135
                     /locus_tag="LGK42_001277"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22093..22095,aa:Gln,seq:ctg)
     gene            22173..22247
                     /locus_tag="LGK42_001278"
     tRNA            22173..22247
                     /locus_tag="LGK42_001278"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22205..22207,aa:Gln,seq:ctg)
     gene            complement(22401..23576)
                     /gene="ubiF"
                     /locus_tag="LGK42_001279"
     CDS             complement(22401..23576)
                     /gene="ubiF"
                     /locus_tag="LGK42_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBK2657546.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23722..25146
                     /gene="miaB"
                     /locus_tag="LGK42_001280"
     CDS             23722..25146
                     /gene="miaB"
                     /locus_tag="LGK42_001280"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBK2657547.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25299..26339
                     /locus_tag="LGK42_001281"
     CDS             25299..26339
                     /locus_tag="LGK42_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK2657548.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26336..26803
                     /gene="ybeY"
                     /locus_tag="LGK42_001282"
     CDS             26336..26803
                     /gene="ybeY"
                     /locus_tag="LGK42_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK2657549.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26893..27771
                     /gene="corC"
                     /locus_tag="LGK42_001283"
     CDS             26893..27771
                     /gene="corC"
                     /locus_tag="LGK42_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBK2657550.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27796..29334
                     /gene="lnt"
                     /locus_tag="LGK42_001284"
     CDS             27796..29334
                     /gene="lnt"
                     /locus_tag="LGK42_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBK2657551.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29412..31289)
                     /locus_tag="LGK42_001285"
     CDS             complement(29412..31289)
                     /locus_tag="LGK42_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK2657552.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLFSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAALATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGAMGWQRARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQIDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            31744..32652
                     /gene="gltI"
                     /locus_tag="LGK42_001286"
     CDS             31744..32652
                     /gene="gltI"
                     /locus_tag="LGK42_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBK2657553.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            32822..33562
                     /gene="gltJ"
                     /locus_tag="LGK42_001287"
     CDS             32822..33562
                     /gene="gltJ"
                     /locus_tag="LGK42_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBK2657554.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            33562..34236
                     /gene="gltK"
                     /locus_tag="LGK42_001288"
     CDS             33562..34236
                     /gene="gltK"
                     /locus_tag="LGK42_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBK2657555.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34236..34961
                     /gene="gltL"
                     /locus_tag="LGK42_001289"
     CDS             34236..34961
                     /gene="gltL"
                     /locus_tag="LGK42_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBK2657556.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35079..36014
                     /gene="rihA"
                     /locus_tag="LGK42_001290"
     CDS             35079..36014
                     /gene="rihA"
                     /locus_tag="LGK42_001290"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBK2657557.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36098..37768
                     /gene="hscC"
                     /locus_tag="LGK42_001291"
     CDS             36098..37768
                     /gene="hscC"
                     /locus_tag="LGK42_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBK2657558.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(37828..39279)
                     /gene="djlC"
                     /locus_tag="LGK42_001292"
     CDS             complement(37828..39279)
                     /gene="djlC"
                     /locus_tag="LGK42_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBK2657559.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39276..39983)
                     /locus_tag="LGK42_001293"
     CDS             complement(39276..39983)
                     /locus_tag="LGK42_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBK2657560.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFMSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9835 a        10251 c        10086 g         9912 t
ORIGIN      
        1 caacttgagg tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca
       61 aaaagcaaac tactgattta tttatcagcg gtggagcttt gctttttttc cggcgtgatc
      121 gatttctcct ttgagaaatt gaggacctgc tattacctga aataaagaga tgaacaaaat
      181 gtcagaatta aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc
      241 atgcgtgaat atagacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcag
      301 tgatgttgaa cgtggaaaac tcgatgaaat agacgctact ggctatgaca ttcctgtttt
      361 tattgcaacg gaaaatgaag aacgtgtacc cgcagaatat ctgccacgta tttctggtgt
      421 cttcgaacat tgcgaatcac gtaaagagtt ttatggacgt cagttagaaa ccgctgccag
      481 ccattatgaa acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgttaatca
      541 gggcaatagt gcttttgact gccctggtca tcaggggggt gagtttttcc gtcgccatcc
      601 ggcaggcaat cagttcgtgg aatactttgg cgaaatgctc ttccgctctg acttgtgtaa
      661 tgctgacgta gcaatgggcg atctgctgat tcatgaaggt gcgccatgta ttgcacaaca
      721 gcatgcggct aaagtcttta atgccgataa aacctatttc gttttaaacg gtacttcatc
      781 ttctaataaa gtggtcttaa acgcattgct gacgccgggc gatctggtgt tgtttgaccg
      841 caataaccat aaatctaacc accacggtgc gttgctacag gctggcgcga caccggttta
      901 tctggaaacc gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga
      961 agaaagttac ctgcgtgagc tgattacaga agttgcaccg cagcgggcaa aagaggctcg
     1021 tcctttccgc ctcgccgtta ttcagctagg cacttacgac ggtacgattt ataacgcccg
     1081 tcaggtggtg gataaaattg gtcatctgtg tgactacatc ctctttgact ccgcatgggt
     1141 cggctatgaa cagtttattc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa
     1201 tgagaacgat ccgggtattc tggttacgca atccgtacat aaacagcagg ctggtttttc
     1261 tcaaacttca caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc
     1321 gcacaaacgc atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct
     1381 gtttgccgca ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg
     1441 gatggattgt gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca
     1501 tattcgtccg ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc
     1561 gcaaattgcg gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt
     1621 tgaaggctat gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc
     1681 aggtattgat gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc
     1741 taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt
     1801 cctgctgact ccggcagaag atatggccaa gcttcagcaa cttgttgccc tgctggtacg
     1861 cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa
     1921 acagcatgaa gagcgctacg ccggttatac cctgcgtcag ttgtgtcaag aaatgcatga
     1981 tttgtatgcc cgtcacaacg tgaaacaact gcaaaaagag atgttccgta aggaacactt
     2041 cccacgcgta agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga
     2101 gctggttcgt ctgccggatg cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc
     2161 tccgggtgtg ctgtgcgttg ttccgggtga aatctggggt ggtgctgttc tgcgttactt
     2221 cagcgctctg gaagaaggga tcaacctgtt gccaggtttt gcaccggagc ttcagggtgt
     2281 ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga
     2341 tgcgcaaagc accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg
     2401 ggcgtcgttc agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg
     2461 ctgccgacaa agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc
     2521 gtaggctcaa tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca
     2581 ggcggtatgg gcggttatgc ggagtatgct ttcggtaaat ccggtaactt tatggcgaac
     2641 tatacctacg gcgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt
     2701 tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc
     2761 ggggtgctgt ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc
     2821 agtagcatta ccgtgtgggg ggtcattatt ccggtggtag gcctgtgcat tatcggctgg
     2881 ttctggttta gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc
     2941 agtgcagtag gttcttccat cgctatgacg ctgtgggctt ttctcggtct ggagtctgcg
     3001 tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc
     3061 ggtacgttag gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg
     3121 cctaatatgg agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttc
     3181 acaccagaag tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg
     3241 cttggctggc agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc
     3301 cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt
     3361 gtgattatcc agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc
     3421 aacgtgctgg ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg
     3481 gcattagtca ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac
     3541 tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa
     3601 gccatgctgt acggttccat cgtgaccttc cttggttgga cactgtatgg tctggtatca
     3661 ccacgctttg aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt
     3721 gcgaccgccc tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct
     3781 caaccgcttc tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga
     3841 tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca
     3901 ctttcagacc gccaatagaa gcaccgttgc ccggagcagc agtcaggcgc gcggtgatcg
     3961 ggtcacctgc cagggtgctc gcgctcacca tttccggaga cagcttagac agcgccgctt
     4021 tttgtgcgga agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg
     4081 ccagttcgtt gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca
     4141 gcagacacat aatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga
     4201 aggaagcccc tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa
     4261 caaaccattt gaaacctacc ggtacttcta ccagcttacg gcccaagtca ttgaccacac
     4321 ggtcgatcat cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg
     4381 gacgatgctg gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac
     4441 ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga
     4501 acttatcacg cagtgccagc agacccgcca tcgcacactc ggaggagcag tccatacgga
     4561 tcgcaccgtc tttatcgagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga
     4621 tggtcaggtt gaggttgtaa tactcgccaa tgcgcttcca gtattcgata ccggaaccgc
     4681 ccaacggatc aacgcccagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat
     4741 cggccagccc ttccacgaac ggctgcacca gatcctgctc tttcacatga ccggatgcca
     4801 tcgcttcgtc gagggagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg
     4861 ccctgtcttc caccactttg gtgacgttag tatcagccgg gccaccattt ggcggattgt
     4921 acttgatacc accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca
     4981 gcgggccacc ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggagtga
     5041 agccattgtt ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg
     5101 aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga
     5161 tgccgttttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt
     5221 taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct
     5281 ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa
     5341 tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct
     5401 ttgtctgcaa cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc
     5461 atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc
     5521 acccaatacg gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc
     5581 gtttgttcat ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg
     5641 gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc
     5701 gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg
     5761 tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct
     5821 ttcgtcaccg gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg
     5881 tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca
     5941 tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca
     6001 ggagtatatg aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg
     6061 gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga
     6121 caacgcgaaa aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac
     6181 cagcacaata attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt
     6241 ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac
     6301 cacggtcttt caccgagaga gccggtaatg aattacccgg cgatggcgca ggatcttgtt
     6361 gataccctgg atgcactgca gatcgacaaa gcaacattta tcggtcactc catgggcggt
     6421 aaagcggtaa tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc
     6481 gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg agattttcgc agctatcaac
     6541 gcggtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat
     6601 cttaatgaag aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc
     6661 tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg
     6721 gcatgggatc accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag
     6781 taccgtgatg atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg
     6841 ggtcactggg tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc
     6901 aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt
     6961 ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct
     7021 gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt
     7081 agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga
     7141 tgaacagctg cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa
     7201 gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc
     7261 gcgcaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct
     7321 gcgtagtcag ggcatcgttt aaatttccag tttcatgtag cacagtgtgc agtcctgctc
     7381 gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt
     7441 tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc actggcatct
     7501 ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg
     7561 gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt
     7621 atgacattct gctgctgggc atcccaacct ggtattatgg cgaagcgcag tgtgactggg
     7681 atgacttctt cccgactctc gaagagattg atttcaatgg caaactggtt gcgctgtttg
     7741 gttgtggtga ccaggaagat tacgccgaat atttctgcga tgcattgggc accatccgcg
     7801 acatcattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt
     7861 tcgaagcatc aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag
     7921 accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag
     7981 agttgcatct cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg
     8041 ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg
     8101 tacctgtaca atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac
     8161 gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat
     8221 aatgatacgc attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt
     8281 agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga
     8341 aagtaacgct tccacgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg
     8401 tcagtgcgga agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta
     8461 cggtatatcg cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg
     8521 aaggcggtaa atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc
     8581 tcgactgcgg caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa
     8641 ttgccgcaaa acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg
     8701 ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg
     8761 agaaaagcca acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta
     8821 cagctctcac ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt
     8881 tcaccgcctg atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc
     8941 gtacactttc ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca
     9001 cgctctggca ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata caggcgctgt
     9061 atgcctcttt cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc
     9121 ccgatgccat tatggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc
     9181 aggcacaaaa cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat
     9241 ttcacgtcac gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtca
     9301 gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc
     9361 tgaatggtgt agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg
     9421 tagtacgcat ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag
     9481 gaagcgccga tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag
     9541 aagaccgctt tttcgccgtt ggcgttgaag tcggaacggt tatcccacca gatatccagg
     9601 cggccgttgg aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg
     9661 tcagccttaa cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca
     9721 aaggtcaacg cttgcagcca ggcggtgccg tcataaaggt cgttgacgct gcgatcgtca
     9781 actttatcgc gcgtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca
     9841 aatttgtagc tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc
     9901 gcttccagta cgaagttatt tttgaagtcg tatttcgccc caagggagtg cagataatca
     9961 actttggtgg ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg
    10021 tattcgttgg tccacatgta ggagaaactc aacgcaccag catcgccgta atcaaaattc
    10081 gccccggctt ccgccccctg ataagtacct ggcataaagc tccagtgcgg cgctaacagc
    10141 gtttgaccag ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg
    10201 gccgcagctt tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct
    10261 ttattacttt ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt
    10321 tcaatcgccg taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga
    10381 aaatcgaggt tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg
    10441 gtaacatctt tacggtcgcg ttcacgctgc cagtaatata taccgccggt taaggttgaa
    10501 tcgtcgatga atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta
    10561 acaccggcga tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa
    10621 tcctctttga cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata aaaattaaaa
    10681 aagtagcgtt gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat
    10741 tatcaatctc tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga
    10801 tttttgttac aaggcttaag tttgcggttg aaaggcttgc agaagaataa aagcgtgaat
    10861 ttccagcaag atgaaagcgg ttacatttta tttaattatg gtttgtgatt ttaaatttat
    10921 tttatggata aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa aacgccgcag
    10981 agtacggcgt ttgggattgt ttgctatttc ttgccggatg cgacgtaaat gcctcacccg
    11041 gcctggtaga acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt
    11101 gcggttaaat accggttttt ccgctgtgga atggcggctg tcgaggcaga agtaaccttc
    11161 acgctcaaac tggaatgctt tacccgcaac cgcatctttc agcgacggtt cagcaaagcc
    11221 ctgtttgatc accagcgatt ccgggttaat caccgacagg aaatcatccg cagcaccagg
    11281 gttcggcacg ctgaacagac ggtcgtacag acggatttca accggcagcg catgtgccgc
    11341 gctcacccag tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt
    11401 gtcggcgtca taagtacaga agatggtggt gatattgcct tcggcatctt tctccacgcg
    11461 ttcagcctta atgacgtaag cattacgcag acgcacttct ttacccagca ccagacgttt
    11521 gtactgcttg ttagcttctt cgcggaaatc ggcgcgatcg atccaaatct caccgctaaa
    11581 cggcacctga cgactcccca tttccggttt gttcggatgg ttcggcatgg tgaccatttc
    11641 gccttcgccc tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg
    11701 cggcgcattt tcgttgagat cttcacggat gcaggattcc agcgatgcca tctcaatggt
    11761 gttgtcctgc ttggtcacgc cgatgcgttt gcagaactca cgaatagaag cagcagtgta
    11821 accacgacga cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg
    11881 cttgtcggtc accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag
    11941 gcgcgagaat tcgtactggc gcgggtgaac aggaatggtg atgttgtcca gcacccagtc
    12001 gtacaggcga cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccag
    12061 ggcatcgctg atgcagtggg tgaagtcgta catcgggtag atgcaccact tgttgccagt
    12121 ctggtggtgt tcagcaaatt taatacggta cagcaccgga tcgcgcatca cgataaacgg
    12181 tgaagccatg tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg
    12241 cattttttcg aacagcgcca ggttctcttc aacgctgcgg tcgcggtacg ggctgttttt
    12301 acccggttgc gtcagggtgc cgcggtattc gcggatctgt tccggcgtca gttcatcaac
    12361 gtacgccagg cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc
    12421 ggaggagtaa cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat
    12481 cgactcaaca tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca
    12541 ctggccttta tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat
    12601 atgcagatag ccattcggct ccggcgggaa acgggtgtgt actgtggtgt gcttaccact
    12661 ggccagatct tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact
    12721 catcgtggat tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac
    12781 aggctgacaa ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg
    12841 atgaaaaaaa aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctca
    12901 agcgtcgcat caggcataaa gcagattact ttttgatttc atacagcggt gtttgacccg
    12961 ccacaacatg gccctgagct ttaatgatca agccactgaa atcgtcgata ttgctgcaaa
    13021 ccaccgggct aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa
    13081 tcggttgccc tgcgcttacc tgcgcaccct cttccaccag acgtttaaag cctttacctt
    13141 ccagcgctac ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca
    13201 ggcagaacgc gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga
    13261 cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt
    13321 cgtcaggaac ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga
    13381 tagataccgc gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt gccggagttg
    13441 cttcagctga cgcagcggct accggaccac gggcaacgac tttcttcatc gcatcgccga
    13501 tggattctgc tttcgcgcca acaatcacct gaatagtctg tttgttcagt ttcactacac
    13561 cagaagcacc cagacgttta cacatcgtat cgttgacgcg ggcagagtca gccacggtca
    13621 ggcgcagacg ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg ccaaccgcag
    13681 caatatagtt ggtcgccagt tgattgaggc cttcttcagt gttgctgttg gcttcttcag
    13741 taacgatctc gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa
    13801 ccaaactgaa caccacgaag tagatagcga agaagacaac acccataacc agcagcatcc
    13861 agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata gcccccgcag
    13921 agaaagagaa gccagcatgg atacccagca gtgttgccac aaacaggctg ataccggtca
    13981 gcagtgcgtg caggaggtac agcagcggtg caaggaacat gaacaggaat tccagcggct
    14041 cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg
    14101 gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca
    14161 tcataatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcaccg gcatagaagc
    14221 ggttgatatc accgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc
    14281 aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac
    14341 caaagatacc ggaacccagc gcgcccgcag aaacgatcca ctcgcctcct gcatggatag
    14401 cgtgctgtac cggcggccag acgtaaccaa gaatggccgc cagcaccagg cagaagaagc
    14461 cggtggcaat cggcacaaag cgtttgccgc cgaagaaact caggaagtcc ggcagtttaa
    14521 tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta
    14581 cacccatgtt aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta
    14641 ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga
    14701 tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa
    14761 cgttaagtaa atctggctga ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga
    14821 tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc
    14881 ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct gcaaattgta
    14941 ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa
    15001 ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat
    15061 cactcccttt tactggctaa accagaaaac ttattttatc attcaaaaaa tcaggtcgga
    15121 ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat gcctaatccg ccaacggctt
    15181 acattttact tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc
    15241 ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc gactgccgat
    15301 cgtccgtttg ttctgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta
    15361 gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac
    15421 gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat
    15481 ttcttcgatc acgttgatat tccagcagaa aacatcaacc ttctcaacgg caacgccccg
    15541 gatatcgacg ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat
    15601 ctgtttatgg gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct
    15661 ctggcttctc gtactcgtat caaaaccttg actcatgaca ctcgcgtcgc gaactctcgt
    15721 ttctttgata acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca
    15781 ttgctggatg ccgaagaagt gatgattctg gtgctgggta gccagaaagc actggcactg
    15841 caggctgcag ttgaaggttg cgtgaaccac atgtggacca tcagctgtct gcaactgcat
    15901 ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta
    15961 agatatttca atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc
    16021 tggctccttg ctcagggcaa tatttttaaa aatcgggggt cagaatgtat gcattaaccc
    16081 agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg
    16141 gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac
    16201 tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg
    16261 tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag
    16321 ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc
    16381 tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg aatcaggcgt
    16441 taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga
    16501 attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca
    16561 ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa
    16621 atgccgggat tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg
    16681 gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg
    16741 gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta
    16801 tcgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg
    16861 acaaactgtg tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca
    16921 tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt
    16981 taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta
    17041 tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg
    17101 agaaacgtct cggcacactc gccgcaggta aagtagccaa cctgaccgca ttcacacctg
    17161 attttaaaat caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag
    17221 tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag
    17281 cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga
    17341 gcaaagccag cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg
    17401 gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt
    17461 caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg acgccaccat
    17521 caccctgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg
    17581 tacccagcaa acgctggaac atgccctgtt gaatgccatt gctcagttta ttgatagcta
    17641 ccagcgcaaa ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc ttgttgaccc
    17701 ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga
    17761 agcactggaa gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc
    17821 gctggcggag cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt
    17881 ccatcgcgga accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg
    17941 caacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg
    18001 caactttggc tgcctggaaa ctatcgctgc caacgccgct attgaacaac gggtgttgaa
    18061 tctgttaaag cagggctacc agagccgcgt gccgctggac gactgcacca tcaaaactat
    18121 ctgcaaagcc gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg
    18181 tcatctgggc aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat
    18241 tgccggtgaa atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa
    18301 tacccaggcg ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg agctggatca
    18361 ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct
    18421 ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct
    18481 gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat
    18541 aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg
    18601 ctggtgttgc tgaccaacta tccttcgcag accgggcaag atctggcgaa ccgctttgcc
    18661 actgccggtg tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc gactgccgat
    18721 ttcctgcgtc gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat
    18781 gaactgtaca aagccggttt cactattacc gatgtgaacc ctgattttgt gattgttggc
    18841 gaaacgcgtt cctacaactg ggacatgatg cataaagcgg cctatttcgt cgctaacggt
    18901 gcacgcttta tcgccaccaa cccggacacc cacgggcgcg gtttttatcc cgcttgtggc
    18961 gcgttgtgtg cagggattga gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc
    19021 agcccgtgga tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga agaaacggtg
    19081 attgtcggcg ataacttgcg taccgatatt ctggcgggct tccaggcggg tctggagacg
    19141 attctggtgc tttctggtgt ttcgtcgctc gacgatatcg acagtatgcc gttccgccca
    19201 agttggattt acccatcggt cgctgaaatc gacgttatct gaatagcaac cacgtctgcg
    19261 gacgtggttt cttattttta cccatcatta aaatcatccc attcaataaa acccgtcaaa
    19321 aattatcgat ccatcaataa atacgccacc acaccctatc ctttttcaca aatcagcaat
    19381 caccattacg ttttttattt tttcaccgcc aaatcgcttg cgcacagtgc tgctttgcgg
    19441 cattttttta aacaagcaaa cacaacaagc aacaaatacc aagttaacgg agaaggttat
    19501 gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc gtaagaaagc
    19561 actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta tttatgccag
    19621 cgataacgcc attctcgctc acgaacgtct gtcaattgtt gacgttaacg cgggggcgca
    19681 acctctctac aaccaacaaa aaacccacgt actggcggta aacggtgaaa tctacaacca
    19741 ccaggcattg cgcgctgaat atggcgatcg ttatcagttc cagacagggt ctgactgtga
    19801 agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact tgcagggcat
    19861 gtttgccttt gccctgtacg acagcgaaaa agatgcctac ctgattggtc gcgaccatct
    19921 ggggatcatc ccactgtata tgggctatga cgaacacggt cagctgtatg tggcctcaga
    19981 aatgaaatcg ctggtgccag tttgccgcac gattaaagag ttcccggcgg ggagctattt
    20041 gtggagccag gacggcgaaa tccgttctta ttatcatcgc gactggttcg actttgatgc
    20101 agtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gctctggaag attccgttaa
    20161 aagccatctg atgtctgatg tgccttacgg tgtgctgctt tctggtggtc tggattcctc
    20221 aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc aggaacgctc
    20281 tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt caccggatct
    20341 taaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa ttcacttcac
    20401 tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa cttatgatgt
    20461 gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca aggcgatggg
    20521 cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt atctttactt
    20581 ccataaagcg cccaacgcta aagaactgca tgaagagacg gtgcgtaaac tgctggccct
    20641 gcatatgtat gactgcgcgc gcgccaacaa agcgatgtcc gcctggggcg tggaagcgcg
    20701 cgttccgttc ctcgacaaaa aattccttga tgtggcgatg cgtattaacc cacaggataa
    20761 aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg agtcatacct
    20821 gcctgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg gttacagttg
    20881 gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcaacagc tggaaactgc
    20941 ccgcttccgc ttcccgtaca acacgccgac gtccaaagaa gcgtatctgt accgggagat
    21001 ctttgaagaa ctgttcccac ttccgagcgc cgctgagtgc gtaccgggcg gtccttccgt
    21061 cgcgtgttct tccgctaaag cgattgagtg ggatgaggcg ttcaagaaaa tggacgatcc
    21121 gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc gaaacagccc
    21181 cggtgaaatc cggggctgtt tcagttattg aggtgcttgt taattctgat atgcagtaag
    21241 gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg ccagtatttc
    21301 atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac gacaaaagaa
    21361 tggctgtagg gtggggaaaa aacacaaagt aaacaataat tgacgaatat agcgccacgc
    21421 tgttcgcaac ctaaccaaac agtcactttc gagcaatttt ccttgaaaaa gaggttgacg
    21481 ctgcaaggct ctatacgcat aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa
    21541 gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    21601 gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga
    21661 tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca
    21721 gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    21781 ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt tcaccgcccg
    21841 gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc
    21901 gaatccaggt accccagcca tcgaagaaac aatctggcta cgtagctcag ttggttagag
    21961 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga
    22021 atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta
    22081 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccaattta
    22141 ttcaagacgc ttacatcgta agtgcgccca gttggggtat cgccaagcgg taaggcaccg
    22201 gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct
    22261 cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga tgttgacacg
    22321 tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac ggttggcacg
    22381 catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac
    22441 acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag cggcggcaga
    22501 ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa gttatccgcc
    22561 atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc accgtagctg
    22621 cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag attcaccccc
    22681 tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag ccccggctgc
    22741 acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg
    22801 taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc catattcata
    22861 ttctgcaact ggcgaatacg taccggagag tcataccaca ccagcgatgc ccagttatca
    22921 aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt gctgtcgccg
    22981 ggatcgttct cacactgtac gctaatcaac atacacgact gcgcatactg ccaggcatga
    23041 acgccaattc ccgccatctg ccgcacctgc gaatttgcgc cgtcagcacc aatcaccagc
    23101 ttcgcgcgaa tcacttcacc gcccttcagt tccagctcct gaagatcatt atctcgatgt
    23161 aatgcaatca gcgagcctgg cacacgtaac gttactttcg gatgagcttc cagcgcctgc
    23221 cacaatgcct gttgcaggac ggtgttttcc accatatagc caagcagcgg cagcttaagt
    23281 tcagcggcgt caaacatcac atgcgccgtt tcccactccc acgtttccag tctgcggtaa
    23341 ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc
    23401 gatgcggcgc tgatcgccga gatccgcacg tccggttggc tatcagcgac aaacggtgca
    23461 ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc cagcgccagt
    23521 gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc
    23581 aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa
    23641 cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca ttcctggcta
    23701 ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag
    23761 atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca cggctatcaa
    23821 ctgaccgacg tggcggaaga agcggatgtg ctgctgctga acacctgctc aatccgcgag
    23881 aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat
    23941 ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga gcacattcgc
    24001 cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag
    24061 atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc
    24121 gagaagtttg accgtctacc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc
    24181 atggaaggct gcaataaata ttgtacctac tgcgtggtgc cttacacccg tggtgaagag
    24241 gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgctgc tcagggcgtg
    24301 cgtgaagtca acctgctcgg tcagaacgtg aacgcctggc gtggtgaaaa ctacgacggc
    24361 accaccggat cgtttgccga tctgctgcgt ctggttgctg cgatcgacgg gatcgatcgt
    24421 attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc
    24481 gacacgcctg agctggtgag cttcctgcat ctgccggtac aaagcggttc cgatcgcatt
    24541 ctgaacctga tgggccgtac tcacacggcg ctggagtaca aagcgatcat ccgtaaactg
    24601 cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt ccctggcgaa
    24661 accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc
    24721 tacagcttta tcttctctgc acgtccgggg acgcccgccg ccgatatggt ggatgatgtt
    24781 ccggaagaag agaagaagca gcgtctgtat attctgcaag agcgcattaa tcagcaagcg
    24841 atggcgtgga gccgccggat gctcggcacc acccagcgta ttctggtaga agggacttcg
    24901 cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt caacttcgag
    24961 ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac
    25021 tctctgcgcg gtaaagtggt gcgtactgaa gatgaaatgg gtctgcgcgt ggcagaaaca
    25081 ccggaatcgg tgattgcccg tacccgcaaa gaaaacgacc ttggcgtggg ttattatcag
    25141 ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt atccccatct
    25201 ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa
    25261 tgttcagctt ccggcgacat acagataaga ggaacggttt gaacatagac actcgcgaaa
    25321 tcaccctgga gccagcagac aacgcgcgtc tgttgagcct gtgcggcccg tttgatgaca
    25381 acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgac aaccacttta
    25441 aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg
    25501 tcgatacagc cccgatgcgc ggtcagattc aggatatcga accggaacag atccaccttg
    25561 cgattaaaga agcgcgagta ctggagcaaa gtgcggagag cgtgccggag tacggcaaag
    25621 cggtcaatat caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt
    25681 acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg ggtacgggta
    25741 aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggag attcgccgta
    25801 ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg cctggcgatt
    25861 taagccagaa agtagacccg tatctgcgcc cgctgtacga cgcgctgttt gaaatgctgg
    25921 gctttgagaa agttgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct
    25981 atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc cagaacacca
    26041 ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca
    26101 ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta cgtcacgcca
    26161 tcgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg
    26221 ttcgtcaccc tgtggtggcg cgtatcgtta acgcctatga agcctgggaa gaagccgaac
    26281 aaaaacgaaa agcggcgctg gcggcagaac gcaagcgcga agaacaggaa caaaaatgag
    26341 tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac cggaagagag
    26401 ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagaat cggaagtgac
    26461 tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc gcggtaagga
    26521 taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg agatgtcgct
    26581 actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa
    26641 accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta
    26701 cgatcacatc gaagatgacg aagcagaaga aatggaagcc ctcgaaacag agattatgct
    26761 tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca ggcatttgac
    26821 tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac
    26881 ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag
    26941 ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa
    27001 ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat
    27061 atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat gatcccccgc
    27121 tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatc
    27181 gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt
    27241 ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac
    27301 aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa
    27361 gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg tggggtttcc
    27421 ggtctggtga ccattgaaga catcctggaa ctaattgttg gtgagattga agacgagtat
    27481 gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca
    27541 ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaggtt
    27601 gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcgcg tggcgaaact
    27661 atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg tattattcag
    27721 gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata aaaccgaaac
    27781 tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat tcgcctgctg
    27841 ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga cgtctggcct
    27901 gcggcgatta tttcgttgat ggggcttcag gcgttgacct ttaaccgccg tccactccag
    27961 tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat taactgggtc
    28021 tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt cctggtggtg
    28081 ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg
    28141 tggccgaaaa ccacctggct gcgcgtggcg attgccgccc cttctctctg gcaagtgacc
    28201 gagtttctgc gcggttgggt actgacaggc ttcccgtggt tacagttcgg ctatagccag
    28261 attgatggcc cgttaaaagg gctggcaccg ataatgggcg tggaagccat taacttcctg
    28321 ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg gcgtccgctg
    28381 gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca atggtttacc
    28441 ccacaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcactg
    28501 aaatgggatg gagatcagct tcttaatacg ctgaagattt actacaacgc aacggctccg
    28561 ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt
    28621 aatcagcaac cgttcctcaa agcactggac ggtgagttgc gcgataaagg tagctcgctc
    28681 gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac ctacaacacc
    28741 atcatcacac tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa
    28801 aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg tccgttagca
    28861 ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca gccgccgctg
    28921 tcggtaaatg gtattgagct tactgcggcc atttgctacg agatcattct tggcgagcaa
    28981 gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa cgatgcgtgg
    29041 tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg tgcgctggag
    29101 ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat tggcccgcag
    29161 ggtgagattc aggcgatgat cccgcagttc acccgcgagg tgttaaccac taatgtgacg
    29221 ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg ggtgctgacg
    29281 gcactgtttg gttttgccgc tgtgctgatg agtctgcgag cacgtaaacg ttgatgtgcc
    29341 tgatgtatcc tgcatgagca aaacgtactt tctcattgag ctttggggca tggttatcat
    29401 gccccttttt cttacggttc agggatacaa gccacattgc catattgggg atgacgaata
    29461 attgcctgcg gatcaagcga gcgcgtcctg tccagttgga tcaacccgtt cttcgccata
    29521 acaaatattt ttctaccatc cgctgaaaaa cgaaggtgat taacgtagct ggcaggataa
    29581 acgccaataa tctgcccggc attaatattg agcaggatga cataaccttg caggccatat
    29641 gacaccaacg ctaaatactg accgtccggg ctaataccct caacggaata aacgctcctg
    29701 ctacaccatt gcgtcataat gctggcttgt tgatccccta cgcttatacg tgaaatcccc
    29761 tggctgtcgg caaaaattac atccccgttt tcaggtcgaa aacgcatcgc cgatatcgtt
    29821 cctcgcgcat cactagccgg atagctttct ttctgatgcg tgatggtatt gatgcgtaca
    29881 atgtcacctc tgaccatacc gggtatcaac aatatttttt catccgggga aagagccata
    29941 acccaggcac caggtttatt actcgtcccc gtcccgtcgt atttctccag cgggatatcc
    30001 gccacatgct gataagtcac cagatcaatc gctgacacac tattactggt gcgattcatc
    30061 acccaggctt ttgtaccatc acgcgagagg ataagactgt caggatgtgc accggtggta
    30121 atcgactgaa taatctcccc tgtcgccacg ttaatgaccg ataacgcatt cgcttcaaat
    30181 gaagtcgcat aaatcaactt ttcatccggg cttaatgcca gcgatctgac gccctgatct
    30241 ttacagccac cacaatgttt gactgacttt tctttcgcta tgggcgctgt aaaggtatgc
    30301 aggattttct tttgtccgag gtcgaaaaca acaatgctgt tggtgtcttc aattgcggca
    30361 tacaacgtat taccatcccg actgatcgcc atatcatgga tatcggcaat ctcagcgagc
    30421 aaatcaccac tggcctgttc acgacttacc ggagcggata atgtttccct gagggcgttg
    30481 cgttcctccg ctaactgttg ttttttttgt cgtcgctctt gttcaatctc ctgcggataa
    30541 aactcttcac gcaggctttg cctgacttgt aaaactgact catcaatctg ctgctgcgca
    30601 agccagatag ccccggtcag aagaagacta cccgcaacaa taatcccacc ttccgtaaca
    30661 agccggtttt gcccagataa acgcgctctc tgccaaccca ttgcgccacc cgcaatcagg
    30721 ccgccaatgt gacaagcatt atctattcct gcttgccggg caccatacaa cagtgtcagc
    30781 gccaccatac ccaacagaga aaatactcgc cgctggtttt tatggtatgt gcccgcacct
    30841 gaagcaagct gtgttgctaa cgcagcgcca gctattccca taattgcccc ggacgcccca
    30901 acgctgatga ctatctgatc actatcgcgt aacgcataac aggcgctgat gagcccacca
    30961 aagacgccgg agaataacca aataatcaga agtcgccatt ttcccagtat ccgttccgcc
    31021 actgccccca ccaccaggag tgcaaacata ttcatcagca aatgggaaaa actactgtgc
    31081 agaaagacac tgctaaataa gcgccaggat tcacccgaca acgtgagaga agcaacgtta
    31141 gcaccgagat ggatgagtgc ggattgatcg acttcttcat aactcatcca ttgtgcgagg
    31201 taacaaaata tcccaacaca gagcgcaaaa aggataaggg ttattgcggg taattgaaca
    31261 tttaacggct taaccgaaga tgcagacata aggtgtttct tccttgaaaa gacaggtaaa
    31321 aagcattttt cccgtgtgca gttatcatcc cggcaaacgt cccatgccga aaagataact
    31381 ctgtccgaaa tatgactttt tcagcctgaa attacaatcg taattaattc acccataagt
    31441 attttttggc atgtaagttg ctttatttac tgtgtaagca atgaaatcag ccgcgacgca
    31501 accgggttgc accaccacaa atcagcggta gcaccaaaag agtgcatttt aagaattttg
    31561 cttcggaatg gtgcggtgca cctcgcaaaa acaaacaata aaacatcaat atctttacat
    31621 ttagccaaac taaatgttac ctaacaagca caacactgca caataaagtt gcagacgata
    31681 acaacacaaa cactcacaac gggtatccat gcgttcttaa cgcagaagat aaaggagttg
    31741 gatatgcaat tacgtaaacc tgccacagca atcctcgccc tggcgctttc cgcaggactg
    31801 gcacaggcag atgacgccgc cccggtagcg ggcagtacgc tggacaaaat cgccaaaaac
    31861 ggcgtgattg tcgtcggtca ccgtgaatct tcagtacctt tctcttatta cgacaatcag
    31921 caaaaagtgg tgggttactc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag
    31981 aaactcaaca aaccggactt gcaggtaaaa ctgattccga ttacctcaca aaaccgtatt
    32041 ccactgctgc aaaacggcac tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa
    32101 cgccaaaaac aggcggcttt ctctgatact attttcgtgg tcggtacgcg cctgctgacc
    32161 aaaaagggcg gcgatatcaa agattttgcc gacctgaaag gcaaagccgt agtcgtcact
    32221 tccggcacta cttctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg
    32281 cgcatcatta gcgccaaaga tcacggtgac tctttccgta ccctggaaag cggtcgtgcc
    32341 gttgccttta tgatggatga cgctctgctg gccggtgaac gtgcgaaagc gaagaaacca
    32401 gacaactggg atatcgtcgg caagccgcag tctcaggaag cctacggttg catgttgcgt
    32461 aaagatgatc cgcagttcaa aaagctgatg gatgacacta tcgctcaggt gcagacttcc
    32521 ggtgaagcgg aaaaatggtt tgataaatgg ttcaaaaatc caattccgcc gaaaaacctg
    32581 aacatgaatt tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc gaatgacaaa
    32641 gcactgaact aattacaaga accaggggcg gaaattccag ccctctcgat tgttacgtag
    32701 cacggacaga ctatacgcct gatggtcgtt ccccatcggg cctgaaaacc gcaatacgct
    32761 gggtaacaat cttcgagggt agcagttaac gctgctaccc tcttttttct ggagtagatt
    32821 tatgtctata gactggaact ggggtatttt tttacaacaa gccccgttcg gcaacaccac
    32881 ctatcttggt tggatctgga gtggttttca ggtgacgatc gctttgtcga tctgcgcctg
    32941 gattatcgct ttcctcgtcg gttcattttt cggcatttta cgtaccgttc caaaccgttt
    33001 tctctccggt ctgggtacgt tgtatgtcga actgttccgc aacgtgccgt tgattgtgca
    33061 attctttacc tggtatctgg tgatcccgga gctgttgccg gagaaaatcg gcatgtggtt
    33121 taaggccgag ctggatccca atattcagtt tttcctttca tccatgctct gcctggggct
    33181 gtttaccgcc gcccgtgttt gcgaacaggt acgcgccgcc attcagtcgt tgccgcgtgg
    33241 gcaaaaaaat gccgcgctgg cgatgggcct gacgctaccg caggcttatc gatacgtgct
    33301 gttgcctaac gcgtatcgcg ttatcgtccc gccgatgact tccgagatga tgaacctggt
    33361 gaaaaactcc gccatcgcct cgactattgg tctggtggat atggcggcgc aggcgggtaa
    33421 attgcttgat tactcagccc acgcctggga gtcgtttacc gctattacgc tggcgtatgt
    33481 gttgattaac gcggttatca tgctggtaat gacgctggtt gaacgtaaag ttcgcctgcc
    33541 tggcaacatg gggggcaaat aatgtacgag tttgactgga gttccattgt cccttccctg
    33601 ccatatctgc tcgacgggct ggtgatcacg ctgaaaatca ccgtcacggc agtcgtgatt
    33661 ggtattttgt ggggcaccat gctggcggtg atgcgcttgt ccagctttgc gcccgtagcg
    33721 tggtttgcca aagcctacgt taacgtattc cgctcgattc ctttagttat ggttttgctg
    33781 tggttttacc tgatcgtgcc aggttttctg caaaacgtgc tgggattatc accaaaaaat
    33841 gatatccgtt taatttcggc gatggtggcg ttttcgatgt ttgaagcggc ctactattca
    33901 gagattatcc gtgctggtat tcaaagtatt tctcgtggtc agtcgagcgc cgcgctggcg
    33961 ttgggaatga ctcactggca gtcgatgaaa ctgattattc tgccgcaggc gttccgcgcg
    34021 atggtgccgc ttctgctcac tcagggcatc gtactgttcc aggatacctc actggtgtat
    34081 gtgttatctc tggccgattt cttccgtacc gcctcaacca ttggtgagcg tgatggtacg
    34141 caggttgaga tgatcctgtt tgccggattt gtttatttcg ttattagtct tagcgcgtcg
    34201 ttgttggtca gctacttgaa aagaaggaca gcataatgat taccctgaaa aatgtttcaa
    34261 aatggtatgg tcactttcag gtgctgaccg actgctcaac cgaagtgaaa aaaggcgaag
    34321 tggtggtggt ttgcggcccg tctggttccg gcaaatcaac gctgattaaa accgtcaacg
    34381 gcctcgaacc agtgcagcaa ggtgaaatca ccgtcgatgg tatcgtggtt aatgacaaga
    34441 aaaccgatct ggcaaagctg cgttcccgcg tcgggatggt attccagcat ttcgagctgt
    34501 tccctcatct gtcgattatc gaaaacctga ccctggcgca ggtgaaagtg cttaaacgcg
    34561 ataaagcgcc cgctcgtgaa aaagcattga aattgctgga gcgcgtggga ctttccgcac
    34621 atgccaataa atttccggcg cagctttccg gcggtcagca gcagcgtgtg gcaatcgctc
    34681 gcgcgttgtg tatggatcct attgcgatgc tgttcgacga accgacatcg gcgctggatc
    34741 cggagatgat caacgaagtg ctggacgtga tggtggaact ggcaaacgaa ggaatgacca
    34801 tgatggtggt gacccacgaa atgggttttg cccgtaaagt ggcgaatcgg gtgatcttta
    34861 tggacgaggg taaaattgtc gaagactcgc cgaaagacgc tttcttcgat gatccgaaat
    34921 cggaccgcgc gaaagacttc ctcgcgaaaa tcctgcatta atcactctgg cgcgcatacc
    34981 cgagtgcgcg ccatctcacg ctttatcgtt tcgcccctcg tatctgttac atcctggcgt
    35041 tacccttgtc gcaaagaagc acacaacaag gagcaacaat ggcactgcca attctgttag
    35101 attgcgaccc aggtcatgac gacgctatcg caatagttct cgccctcgcc tcaccagagc
    35161 ttgatgtcaa agcaattacg tcttccgccg gaaaccagac accagaaaaa accttacgca
    35221 atgttcttcg tatgctgacc ttgcttaatc gcaccgatat tccggtagca ggcggcgcgg
    35281 taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt gcacggcgaa agcggtctgg
    35341 atggcccggc attaccggaa ccgacgttcg caccgcaaaa ctgtacggcg gtagagctga
    35401 tggcgaaaac gctgcgcgaa agtgcggaac ctgtcacgat tgtgtctacc ggaccgcaaa
    35461 ctaacgttgc cttgctgctc aatagccatc cggaactgca tagcaaaatt gcccgtatcg
    35521 tgattatggg cggcgcaatg gggcttggta actggacgcc tgcggctgaa tttaatattt
    35581 acgttgaccc ggaagcggca gaaattgtct tccagtcagg gatcccagtg gtgatggccg
    35641 gtctggatgt tactcacaaa gcacaaatcc acgttgaaga caccgagcgt ttccgcgcga
    35701 ttggcaaccc tgtttcaacc attgttgccg aactgctgga tttcttcctc gaatatcata
    35761 aagacgaaaa atggggcttt gtcggtgcac cgctgcatga cccatgcacc atcgcctggc
    35821 tgttgaaacc cgagttgttt acctctgttg agcgctgggt tggcgtggaa acacagggga
    35881 aatatagcca gggtatgacg gttgttgatt attattatct gactggcaat aaaccgaatg
    35941 ccactgtaat ggtcgatgtt gatcgtcagg gctttgttga tttactggct gatcgtctga
    36001 aattttacgc ttaacacacg tcattgatta agatcaccac gcgttacctg ggtggtctta
    36061 atctgtttat ctctttttct ttcaagatat aaatgcaatg gataatgcag aactcgccat
    36121 tggtatcgat ctcggcacta ccaatagttt aattgccgtc tggaaagacg gtgccgcgca
    36181 attaattcca aataagttcg gtgaatattt aacaccatcc ataattagca tggatgaaaa
    36241 taatcatatt ttagtcggaa aaccggctgt atcacggcgt acttcgcatc cggataaaac
    36301 ggcagcgtta tttaaacgtg caatgggcag taataccaac tggcggttag gcagcgacac
    36361 atttaacgcg ccagaactgt cctctttggt attacgctca ttaaaagaag atgccgaaga
    36421 atttctgcaa cgtccgatta aagatgtggt gatctccgtt ccggcttatt tcagcgatga
    36481 acaacgcaag catacccgtt tagcagcgga gttagccggg ttaaatgcgg tacgcttaat
    36541 taatgaaccc acagcagctg cgatggcgta tggcctgcat acccaacaaa atacccgttc
    36601 gctggtgttt gatctcggtg gcggcacgtt tgacgttacg gtgcttgagt acgccacgcc
    36661 ggtgattgaa gttcacgcct ccgctggcga caactttctt ggtggcgaag attttaccca
    36721 tatgctggtc gatgaggttt taaaacgcgc ggatgtcgcc aggaccacgc ttaacgagag
    36781 tgaactggga gccttgtacg cctgtgtgga agcggcaaaa tgtagcaatc aatcgccatt
    36841 gcacattcgc tggcagtacc aggatgaaac gcgggagtgc gaattttacg agaacgaact
    36901 ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg ccgattgaac aggcgttgcg
    36961 cgatgcacgt ctgaagccga gtcaaatcga cagtctggtg ctggttggcg gcgcgtcaca
    37021 aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc aaattaccgt atcaaagtta
    37081 cgatccgagc accattgtcg cgctgggcgc agcaatccag gccgcctgcc gcttacgcag
    37141 tgaagatatt gaagaggtaa tcctcactga tatttgccct tactcgttgg gcgttgaagt
    37201 taaccgccag ggcgtttccg gcattttctc gccgattatt gaacgaaaca ccactgtgcc
    37261 cgtgtcgcgt gtagaaactt attcaaccat gcacccggaa caggattcaa ttacggttaa
    37321 cgtctatcag ggagaaaacc acaaagttaa aaacaacatt ctggtggaat ccttcgatgt
    37381 gccgttgaag aaaaccgggg cttatcagtc gattgatatt cgctttagtt atgatatcaa
    37441 cgggttgctt gaagttgacg tgcttctgga agacggcagc gttaagtcca gagtgattaa
    37501 ccacagcccg gtaacattga gcgcgcagca gattgaagag agtcggacgc ggttatccgc
    37561 attgaaaatt tatccgcgcg atatgctcat caatcgcacc tttaaagcca aactggaaga
    37621 gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt ggccgggtga tcaccgattt
    37681 tgatgcggcg ctgcagtcaa acgatatggc ccgcgtcgat gaggtcaggc ggcgggcgag
    37741 cgtttattta gccattgaga cctcataatc acggctgccg gatgcggcgt aaatgctgca
    37801 tccggcatcg gcgcaaaaaa cacatcgtca actaaacaac atcgcgattt tgccgagagc
    37861 ataacctata accagcaccg ttaaactgat tttgataatg ctggtaatgc tctgtggttt
    37921 ttcaccctca tcattttttt cttcatggtt ttcaggtgtt ggctcatcga catcttcatc
    37981 ctccggctcg agttttactt tcgtcaacag caaccgggtg ataatggcct ctaatggatg
    38041 agcatctccg ccttccagcc ttgtattaag acgggccagc atctcttgtt tcggaaacgc
    38101 ttcaagtact acctgttccg ctacccgata caaatccagt aacggtgaat cttcttctgt
    38161 ctcgctaccg actctcgatt tccaccacgc ccatgctttg cgtttgaaga ataacgactc
    38221 aataaacgat tgacctaacg gcgataattt tccgctatgt aaaatttccg cccataacag
    38281 ccgcgtccgg gcgctctcac caggaacata tttcagcggt tgaccttgcg catctaccat
    38341 gctccgcgct tcgataacca tcactaataa cgcgaaataa tccgggcaat gacgcttgct
    38401 ccactgcaac aacaaattat cagcttgtgt agatgggtaa ctttcccggt acgcgcagag
    38461 atcggcaagc aggctttccc cctcgccgca gtacaacctt tgagcacaca agtaatatcg
    38521 ggcatcttcg ctttctggtt cttccatttg ccactgtttg gctacaggaa tgagttccgc
    38581 catacctgtt tgcaccgagc tgtaccagcg caacagtttg cgatgtaact ttgcatcatc
    38641 aggaatgaac cacggacctt ccatcgccaa aaacgcagtg acgtagtcag gatggtaacg
    38701 gcagcaacgt tccagcgcga agtaatactc aacagtctgg ttctgcaacg caacgggata
    38761 ttccagaagg ctaaggaaat cgaaacaatc accagcctga atggcctcaa gaaaagcctc
    38821 cctttcttcc tcatccattc cttcgctgtc attaaacgaa tgccagttca aacgctctgc
    38881 cagcaaagaa gcacaattta atgaaagata tcgcgcttct atagcgattg cacacagcgg
    38941 ccagcgtaat tgatcgacat cctccatgtt ccaggtattt aattgctgaa taaatttctg
    39001 ccaggcggag ggctgaaaac gatcactttc tgaatccagt aatgtcctga atgcacgcag
    39061 tatttcatgt tcagcggcgg catctttttc ttcatcatct gcttcctcga caggatttac
    39121 cgccagtcgc agcgcctctt cataggcctg gcgtaatgct ttaaacccct gcggatcggt
    39181 ttcgggatga cataacggta agcgagcaag ataagcctgg cggataatgt ctatttgcgt
    39241 cgtgctttca atttcgagga tttgccaaca tgttttcata ggtcgctcac atccttaagc
    39301 gttaccgggc attcaggctg gacagttaat ggctgccagg gaagcgtggc atagaaacga
    39361 tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg cgctatcgta tttgtacagg
    39421 gttagcgcct cgcgtagcga agtgttgtct tcgcactgag aagattgcca gtagagacgt
    39481 cctaacgaat aggcggaaaa atagtgcatc cagctgtgat agtaatggtg agctcgtaca
    39541 tagacgcacg attgcagcca taaactctct tcttccgtca gccagttgtt gagcacaccg
    39601 acccgtgaca taaaccccat tcgaacataa tcccaggctt tgattccccc ttctccacag
    39661 cacatagcgg tttgggcaac gaattgggta taaatcaacc ctctctccga gccaccagca
    39721 attaatgctt tccactcttc aggggaagag cgatgccatg tcagatataa gccagctaaa
    39781 tcattggcat gaccatcatc agtcatctga taaatagtct gaattaaatc atcacgatta
    39841 tttattcccc aggatgatga gagatcgaat tcttttatcc cggtatacat ttttggcgag
    39901 tcatgcgcgg gtttgcaacc ctgattaagt atttccatgg gcgcagaaag cgcaaaaaga
    39961 agatattgtt cttccttatt catttgttaa taatccctta agtgcattaa ctgactactt
    40021 tttattattg atgagtataa acttgacgct ttcaaaataa aaagaaaatc gaagcattca
    40081 cata
//
LOCUS       DAFAVF010000025        38510 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFAVF010000025 DAFAVF010000000
VERSION     DAFAVF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.025"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            138..2381
                     /gene="pdeF"
                     /locus_tag="LGK42_001294"
     CDS             138..2381
                     /gene="pdeF"
                     /locus_tag="LGK42_001294"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBK2657561.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2420..3961)
                     /gene="ppx"
                     /locus_tag="LGK42_001295"
     CDS             complement(2420..3961)
                     /gene="ppx"
                     /locus_tag="LGK42_001295"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBK2657562.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3966..6032)
                     /gene="ppk1"
                     /locus_tag="LGK42_001296"
     CDS             complement(3966..6032)
                     /gene="ppk1"
                     /locus_tag="LGK42_001296"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBK2657563.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6203..6841)
                     /gene="purN"
                     /locus_tag="LGK42_001297"
     CDS             complement(6203..6841)
                     /gene="purN"
                     /locus_tag="LGK42_001297"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK2657564.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6841..7878)
                     /gene="purM"
                     /locus_tag="LGK42_001298"
     CDS             complement(6841..7878)
                     /gene="purM"
                     /locus_tag="LGK42_001298"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK2657565.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8203..8829
                     /gene="upp"
                     /locus_tag="LGK42_001299"
     CDS             8203..8829
                     /gene="upp"
                     /locus_tag="LGK42_001299"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK2657566.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8915..10204
                     /gene="uraA"
                     /locus_tag="LGK42_001300"
     CDS             8915..10204
                     /gene="uraA"
                     /locus_tag="LGK42_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK2657567.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10254..11000
                     /gene="hda"
                     /locus_tag="LGK42_001301"
     CDS             10254..11000
                     /gene="hda"
                     /locus_tag="LGK42_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBK2657568.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11138..11497)
                     /gene="arsC"
                     /locus_tag="LGK42_001302"
     CDS             complement(11138..11497)
                     /gene="arsC"
                     /locus_tag="LGK42_001302"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBK2657569.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11518..12981)
                     /gene="bepA"
                     /locus_tag="LGK42_001303"
     CDS             complement(11518..12981)
                     /gene="bepA"
                     /locus_tag="LGK42_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBK2657570.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13194..14255
                     /locus_tag="LGK42_001304"
     CDS             13194..14255
                     /locus_tag="LGK42_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK2657571.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14293..15140)
                     /locus_tag="LGK42_001305"
                     /pseudo
     CDS             complement(14293..15140)
                     /locus_tag="LGK42_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(15162..17174)
                     /gene="hyfR"
                     /locus_tag="LGK42_001306"
                     /pseudo
     CDS             complement(15162..17174)
                     /gene="hyfR"
                     /locus_tag="LGK42_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(17204..17617)
                     /gene="hyfJ"
                     /locus_tag="LGK42_001307"
     CDS             complement(17204..17617)
                     /gene="hyfJ"
                     /locus_tag="LGK42_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBK2657572.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17610..18367)
                     /gene="hyfI"
                     /locus_tag="LGK42_001308"
                     /pseudo
     CDS             complement(17610..18367)
                     /gene="hyfI"
                     /locus_tag="LGK42_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18364..18909)
                     /gene="hyfH"
                     /locus_tag="LGK42_001309"
     CDS             complement(18364..18909)
                     /gene="hyfH"
                     /locus_tag="LGK42_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBK2657573.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18919..20586)
                     /gene="hyfG"
                     /locus_tag="LGK42_001310"
     CDS             complement(18919..20586)
                     /gene="hyfG"
                     /locus_tag="LGK42_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBK2657574.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDHVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20576..22156)
                     /gene="hyfF"
                     /locus_tag="LGK42_001311"
     CDS             complement(20576..22156)
                     /gene="hyfF"
                     /locus_tag="LGK42_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBK2657575.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22161..22811)
                     /gene="hyfE"
                     /locus_tag="LGK42_001312"
     CDS             complement(22161..22811)
                     /gene="hyfE"
                     /locus_tag="LGK42_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBK2657576.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22823..24054)
                     /locus_tag="LGK42_001313"
                     /pseudo
     CDS             complement(22823..24054)
                     /locus_tag="LGK42_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24071..25018)
                     /gene="hyfC"
                     /locus_tag="LGK42_001314"
     CDS             complement(24071..25018)
                     /gene="hyfC"
                     /locus_tag="LGK42_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBK2657577.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25029..27047)
                     /gene="hyfB"
                     /locus_tag="LGK42_001315"
     CDS             complement(25029..27047)
                     /gene="hyfB"
                     /locus_tag="LGK42_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBK2657578.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVAHFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSRFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27047..27664)
                     /gene="hyfA"
                     /locus_tag="LGK42_001316"
     CDS             complement(27047..27664)
                     /gene="hyfA"
                     /locus_tag="LGK42_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBK2657579.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27917..28387)
                     /gene="bcp"
                     /locus_tag="LGK42_001317"
     CDS             complement(27917..28387)
                     /gene="bcp"
                     /locus_tag="LGK42_001317"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBK2657580.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28387..28959)
                     /locus_tag="LGK42_001318"
     CDS             complement(28387..28959)
                     /locus_tag="LGK42_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBK2657581.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29105..29983
                     /gene="dapA"
                     /locus_tag="LGK42_001319"
     CDS             29105..29983
                     /gene="dapA"
                     /locus_tag="LGK42_001319"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK2657582.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30000..31034
                     /gene="bamC"
                     /locus_tag="LGK42_001320"
     CDS             30000..31034
                     /gene="bamC"
                     /locus_tag="LGK42_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBK2657583.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31247..31960
                     /gene="purC"
                     /locus_tag="LGK42_001321"
     CDS             31247..31960
                     /gene="purC"
                     /locus_tag="LGK42_001321"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK2657584.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32114..32341
                     /gene="vapB"
                     /locus_tag="LGK42_001322"
     CDS             32114..32341
                     /gene="vapB"
                     /locus_tag="LGK42_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBK2657585.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32341..32739
                     /gene="vapC"
                     /locus_tag="LGK42_001323"
     CDS             32341..32739
                     /gene="vapC"
                     /locus_tag="LGK42_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBK2657586.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32886..33749
                     /locus_tag="LGK42_001324"
     CDS             32886..33749
                     /locus_tag="LGK42_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBK2657587.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33764..35779
                     /gene="tmcA"
                     /locus_tag="LGK42_001325"
     CDS             33764..35779
                     /gene="tmcA"
                     /locus_tag="LGK42_001325"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBK2657588.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALCALNDVRTERLRDRITQWQFFH"
     gene            35853..36551
                     /gene="ypfH"
                     /locus_tag="LGK42_001326"
     CDS             35853..36551
                     /gene="ypfH"
                     /locus_tag="LGK42_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2657589.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36661..36861)
                     /locus_tag="LGK42_001327"
     CDS             complement(36661..36861)
                     /locus_tag="LGK42_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBK2657590.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36889..38016)
                     /gene="dapE"
                     /locus_tag="LGK42_001328"
     CDS             complement(36889..38016)
                     /gene="dapE"
                     /locus_tag="LGK42_001328"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBK2657591.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38020..38376)
                     /locus_tag="LGK42_001329"
     CDS             complement(38020..38376)
                     /locus_tag="LGK42_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBK2657592.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9385 a         9913 c        10111 g         9101 t
ORIGIN      
        1 tatatatcaa ttggaacttt catatgacac ttagaatcaa tgctctctat agtgatgaat
       61 aatcatcatt cgaagtcagg tgggatgcct gtctgaatac acctccttca ggatgtgggg
      121 gattctgctg agcatctatg aaactgaatg caacttatat aaaaatacgt gataaatggt
      181 gggggcttcc gctgttcctg ccttctttaa tcttgcccat tttcgcccac attaatactt
      241 tcgcgcatat ttcttccggt gaggtttttc tcttttatct gcctttggca ctgatgatca
      301 gcatgatgat gtttttcagc tgggcggcat tgccagggat caccttaggg atttttgtcc
      361 gcaaatatgc agagctgggt ttttacgaaa cgctctcatt aacggctaat tttattatca
      421 ttatcattct ctgttggggc ggttacaggg tctttactcc ccggcgtaac aacgtttcac
      481 atggtgatac ccgtttaatt tcccagcgta tattctggca gatagtgttt cctgcaacgc
      541 tgtttctgat acttttccag tttgctgcat ttgtaggatt gctggcgagc agagaaaatc
      601 tggtcggtgt catgcctttt aacctcggga ctttaatcaa ttatcaggcc ttactggtgg
      661 gtaatctgat cggtgtcccg ctgtgctact tcatcattcg ggtagtgcga aatccatttt
      721 atttacgtag ctattattcg caattaaaac agcaggttga tgccaaagtc accaaaaaag
      781 agttcgcgct ctggctactg gcattaggtg ctttattgtt gctgttatgc atgccgttaa
      841 atgaaaaaag cacaattttt agcaccaatt ataccttgtc attattgctg cccctgatga
      901 tgtggggagc gatgcgctat ggttataagc tgatttcgct gctctgggcg gtcgtgttga
      961 tgatcagcat ccacagctat caaaattaca ttcccattta tcctggctat accacgcagc
     1021 tgaccataac ctcctccagt tatctggtat tctcttttat tgtcaattat atggctgtac
     1081 tggcaactcg tcagcgagcg gtagtcagac gcattcagcg gcttgcgtat gtggacccgg
     1141 tggttcatct gccaaatgtt cgcgccctga atcgcgcgtt gcgtgatgcc ccctggtctg
     1201 cgctttgtta tttacgcatc cctggcatgg aaatgctggt taagaactat ggcatcatgc
     1261 tgcggattca atacaagcaa aaactttctc actggctgtc acccttgctg gaaccgggtg
     1321 aagatgttta tcagctttcg ggtaacgatc tcgcgctgcg gctgaataca gaatcgcacc
     1381 aggagcgcat taccgcactg gatagccatc tcaagcaatt tcgtttcttt tgggatggca
     1441 tgccgatgca accgcagatt ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata
     1501 tctacctgct gctgggagag ctaaatacgg tcgccgaact ttccatcgtg accaacgccc
     1561 cggaaaatat gcagcgtcgc ggggcaatgt atttgcaacg cgaattgaaa gataaagtcg
     1621 cgatgatgaa tcgactacag caggcgctgg aacacaacca ttttttcctg atggcccagc
     1681 cgattaccgg tatgcgtggt gatgtttacc atgaaattct tctgcgcatg aaaggtgaga
     1741 atgatgaact gatcggcccc gatagcttct tgccggtcgc gcacgaattt ggtttatcgt
     1801 cgagtatcga catgtgggtc attgagcata cgctgcaatt tatggctgaa aacagagcga
     1861 agatgcccgc tcaccgtttt gctattaatc tgtctccaac ctcggtatgt caggctcgtt
     1921 ttcctgttga agtcagtcag ttgctggata aatatcagat tgaagcgtgg caacttattt
     1981 ttgaagtcac cgaaagtaat gctctgacca atgttaagca ggcgcaaatc accttgcagc
     2041 atcttcagga attaggctgc cagattgcga ttgatgattt cggcaccggc tacgccagct
     2101 atgcgcggct taaaaatgtg aatgccgatc tgcttaaaat tgacggcagt tttatccgca
     2161 atattgtgtc aaatagtctg gattatcaga tagtggcatc gatttgccac ctggcgcgaa
     2221 tgaagaaaat gcgggtagtg gcagagtacg ttgaaaacga agagatccgc gaggcggtgc
     2281 tctctttggg gatcgattat atgcagggtt atcttattgg taagccgcaa ccgttaattg
     2341 atacgctgaa tgaaatcgaa cccattcgcg aaagtgcctg aataatgcgg gccgacattt
     2401 ctcgtcggcc cgcaaagcat taagcggcga tttcaggtgt actttcttct tcaattttca
     2461 accgccagcc agccacgcct tcccagtatt cttgctcctt ttccagatca agcagtacca
     2521 gcgcattctg actaaaccag tcatgcggga aacgcagtgt ccagtgactg tcatcagtaa
     2581 tcagtgtcaa tgttggcggt gtggtggttg cctgacgttg attgtttagt aatacgccaa
     2641 ggcgcaatag ctgtatcagt ggcaggaact gtttcttctt aaacaaggta aagcgcggca
     2701 gatcgtcgag cttaatcgct ttacggtgat agcgcaccag tgtcgccatc atcagctgct
     2761 gttcctgatt aaaacccggc aagtcactgt tttgcagaat ataagcggag tggcgatgca
     2821 aaccgctgtg gttgatattc aacccgacct catgcagcat ggcggcccat cgcagtagcg
     2881 cctccagttg cggatgcgcc agcttcggtt gctgttcccg ccactgttcg tacatttgca
     2941 tagtggtatc cagcactcgt cgggcctgtt cgctgtcgat gtgatactgg ttggcgaggc
     3001 tgctggcggt acgactacgc acatcctgat gacggaaacg tccttccatt tcatacagta
     3061 cgccttcacg taaagccccg tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca
     3121 caccgcataa aatcgccagt ccaggaacga agactgtttt ccgctcttcg gaaagacccg
     3181 gtaaactcag cgatgcgaaa ttacggtgac gtaaaacttc ttttaccagt ttttccagac
     3241 gttccggggt aattatcccg tctttctcgc ccatttccat taatacttca tgggcggctt
     3301 ttatggtacc ggaagcgccc attgccacgt tccagccctg aatacggaat tgccaggtta
     3361 aagtttccag tttttgtgct gccgccatgc gagcgcgctg gaaattctct ttattgatga
     3421 ccccgccagg gaaataaagc tgggcaaagc tgacacaacc catccggcgg ctttcaacga
     3481 gaataggttc gaaattttca ccaatcacca gttccgtaga tccgccgcca atatcaataa
     3541 ccagtttgcg acctttttcc ggttgggtat gttccactcc cataaaaatc agacgggctt
     3601 cttcattacc ggaaataatt tcaatcgggt agggaatgac cttttccgcg cgtttcagaa
     3661 agtcagtggc gttcagcgcc tgacgcagcg tatgggtacc aactatacag acgctggcag
     3721 gagaaaaccc ttgtagccgt tcggcaaaaa gcgacagaca gttcaaaccg cgcgtcattg
     3781 cctcttcact caacatatta tctggcccca ggccgtccgc cagatgcacc cgctgtttca
     3841 ggcggccaat aatctgcatg gcaccatcta ccacacgggc tatgaccatg tgaaaactgt
     3901 ttgaaccaag atcgaccgca gcaaactcct gcggacgagg ggatttatcg tgtattggca
     3961 tagggttatt caggttgttc gagtgatttg atgtagtcat aaatcgccaa ctgcgcccgt
     4021 actttgcggc gattgccgcg gggaacgtag cgattactga gttctttatc gatataacga
     4081 gctttgaccg tatcgctgaa caatatgtcg atgatgtcca gcacccgctg cttcaggcgc
     4141 ggatcgagca gcggcgtcgc cacttcaata cgataatcaa tattgcgcgt catccagtcg
     4201 gcggaagaaa ggtagacctt tttatcgccg ccattttcaa aaatataaac ccggtcatgt
     4261 tcaaggtaac ggtcaacaat actgatggca cgaatgttgt cgctaatgcc ttccagattg
     4321 gggatcagcg aacacattcc gcgaaccagc agattaaccg gtacgccgga gctggaggcc
     4381 gcatacagac gatcaaccag gcctttatcg acaaggttat ttagcttcag ggtgataccg
     4441 ctgggcagcc cttgctgcgc gttggcgatt tcgcggtcta ccatttcata taacagacgg
     4501 cgagagtttt gcggtgacac cattaaataa tcaaatgtca ccgggcggta tgggttttca
     4561 ataaagttaa atacccgccg tacttcgttg gtgatacgcg catcggcggt cagcaatgaa
     4621 tagtcagtat aaagacgcgc ggttttttcg ttaaagttcc cggtcccgat gtgtgcgtaa
     4681 cgcaccactt caccgttttc ttttcgtgaa atcaggaaca gtttggcgtg aattttcagc
     4741 cccggcgcag agaagataac gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg
     4801 ttagcttctt cgtcgaaacg cgcctgtaac tcaaccacca cggtgacttt cttaccgtta
     4861 tgtgcggcgt ggatcatcga gtcgatgatg cgtgaatctt tcgccacgcg gtaaatgtta
     4921 attttaatcg ccagtacgct cgggtcgaac gaagcctgac gcagcagttc cagcacatgc
     4981 tcaaaggtgt gataaggata atagagcaac acatcgcgtt cgcgaatggc atcaaaacca
     5041 ttgcggaact gggctttatc aaaccaaata tggcgtaaac gcggcagtgg tttgttcacc
     5101 agattggctt tgccgacatt ggggaaatta ataaagtctt taaaattatg ataacgaccg
     5161 ccggggacga tggagtcgta gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc
     5221 gcattgggca tatcgcgctg ataaacaaaa cgcaccggct cagcagttaa acgctgcttg
     5281 agactggaag acatcaactc catcaggctg gcttccatct catgcactaa atcgtattcg
     5341 gcatcgcggg tcatcttcat tgaataggca ttcagcgcgt cataatcaaa gaagcctttg
     5401 aaaatatcat caaggcagta acgcagaatg ttatccagaa gaatcatcgg cttgcgtcga
     5461 cgcggcgctt ctggcggtaa attcacaaag cgcggcactt tatctgatgg gatctccagc
     5521 agcgcgtaac ggatggtatc gccacggata atttccaccg ccagataggt gtaatcatct
     5581 ttcaggaact gcactaagtc agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc
     5641 agatactgct taaaataatg acgcagccag ttttgttgat tgacggagag ctggcgttca
     5701 ttaatcagga agatctggtt acgcgccatc tccagcagca gctcgttgta gaggccgtcg
     5761 aattcctgat cggctttcag cacccgggac tgaattttgc ccagtaaatg gcgggaatga
     5821 gagttggagc cttgttcttc gctaataatg atgcgtcgct tcagttcagc gaagcggact
     5881 ttatagaact catcaaggtt attggaatag atccccagga aacgcatcct ttcaatcagc
     5941 gggttagatt tgtccgccgc ttcctgaagc acgcgttcat tgaacgataa ccaactgagc
     6001 tctttttcga tgtatagctt ttcctgaccc attaccactt ttactccgtt ttattcacgg
     6061 gacactgcct ggatattatg gcgagcaata ttggcagatg tccaactatg ccagaaaagg
     6121 gggaaaaaaa ctgctggagc ttgcgattta caggtgaatt gaaaaacgcc agcggcagag
     6181 ctggcgcttt aactacgggg cattactcgt cggcagcgta gccctgcggc ggcagacgtt
     6241 gaccatccag ccacgcggcg ttttcgtgca ttttcagacg accatcggca aaccagctaa
     6301 tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg ggcggtgatg tcatcttccg
     6361 tatcaccagc aaataccggg actttcgcct gtaaaataac cgggccaccg tccagttcat
     6421 cggtgacgaa atgcaccgat gtaccgtgct cttcatcacc attttccagc gcctgacgat
     6481 gggtgtgtaa tccgggatat ttcggcagca gagaagggtg aatgttcagc aaacgcccgg
     6541 catagtggga gacaaacgcc gggctgagaa tgcgcataaa accagccagc acgaccacat
     6601 cgggtgcgta catgtcgatt tcatgaatca actcccggtc ataggcctca cgactgtcaa
     6661 acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg gcgggcgcgt tcaaggccga
     6721 acgcgtcggc cttattgctg aaaactgccc gtacggtgcc tttaattttg ttggttttac
     6781 aggcgtcaat aattgcctgt aaattacttc cgttgccgga aataagcacc acaatattca
     6841 ttattcgata accacgcgtt gttcggaatc agaggctttg atgataccga ttttccacgc
     6901 gttttcaccg ttggcattga gtagggcgag ggctttgtcc acttccggag caggcagggc
     6961 aataatcatc ccgacgccgc agttgaaggt gcgatacatt tcatggcgct caacgttacc
     7021 tgccgtttgc agccagttga acacttccgg ccactgccag gaagattcat caatcactgc
     7081 ctgggtatta tctggcaata cgcgcggaat gttttcccag aagccgccgc cggtcaggtg
     7141 cgcaatggca tgcacatcga ccttttcaat caactccagc actgacttca cgtaaatgcg
     7201 ggtcggtgcc agcagatgat cggctaatgg cttaccatca agttcggtgg tttgcggatc
     7261 acaaccgctg acttcaagaa ttttgcgcac cagcgaatag ccgttcgagt gcggaccgct
     7321 ggaaccgagt gcaatcagca catcgccgtc gctgacttta gagccgtcga tgatttctga
     7381 tttttctacc acgccaacgc agaaacccgc gacatcgtaa tcttcaccgt gatacatccc
     7441 cggcatttct gccgtttcgc cacccaccag tgaacagccc gattgcagac atccttccgc
     7501 aatgccgctg atcaccgctg aagcggtatc aacatccagt tttccggttg cgtaatagtc
     7561 gaggaaaaac agcggttctg caccttgcac caccaggtca ttaacgcaca tggcgaccag
     7621 atcaatacca atggtgtcgt gacgttttaa gtccattgcc agacgcagct tggtacctac
     7681 gccgtcagtg ccggaaacca gcacgggttc acgatatttt tgcggcaatg cacacagcgc
     7741 accgaagccg cccagaccgc ccatcacttc cggacgacgc gttttcttca ctacgccttt
     7801 gattcttcca accagagcat tacccgcgtc aatatcaaca ccggcatctt tgtagctaag
     7861 agaggtttta tcggtcactg cttgggtccc cacgcgttac ttgcggtaga aaaataaaat
     7921 tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac tgctttacac aacctttttg
     7981 cacgtctttt ccccagacgc gcggcgaaag aagacttgtg ccagggcaaa ggttagtttt
     8041 cggatggaat aatcttcttt cataaccatt tgaatataaa ataactttat ctcaaaccgt
     8101 tatcattttg actaaagtca acgaaaagaa tattgccgcc ttgaagaaag gaggtataat
     8161 ccgtcgattt tttttgtggc tgcccctcaa aggagaaaga gtatgaagat cgtggaagtc
     8221 aaacacccac tcgtcaaaca caagctggga ctgatgcgtg agcaagatat cagcaccaag
     8281 cgctttcgcg aactcgcttc cgaagtgggt agcctgctga cttacgaagc gaccgccgac
     8341 ctcgaaacgg aaaaagtaac tatcgaaggc tggaacggcc cggtagaaat cgaccagatc
     8401 aaaggtaaga aaattaccgt tgtgccaatt ctgcgtgccg gtctggggat gatggacggt
     8461 gtgctggaaa atgttccgag cgcgcgcatc agcgttgtcg gtatgtaccg taatgaagaa
     8521 acgctggagc cggtaccgta cttccagaaa ctggtttcta acatcgatga gcgtatggcg
     8581 ctgatcgttg acccaatgct ggcaactggt ggttccgtta tcgcgaccat cgacctgctg
     8641 aaaaaagcgg gctgcagcag catcaaagtt ctggtgctgg tagctgcgcc agaaggtatc
     8701 gctgcgctgg aaaaagcgca cccggacgtc gaactgtata ccgcatcgat tgaccaggga
     8761 ctgaacgagc acggatacat tattccgggc ctcggcgatg ccggtgacaa aatctttggt
     8821 acgaaataaa gaataaaaat aattaaagcc gactttaaga gtcggctttt ttttgagtaa
     8881 agcgcctata acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag
     8941 tgaaagaccg ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt
     9001 tggtgcaacc gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt
     9061 taacggtatt ggaacgctgc tgtatctctt catctgtaaa gggaaaattc cggcttatct
     9121 tggttccagc tttgccttta tttcaccggt attgttactg ttgccgttag ggtatgaagt
     9181 cgcgctgggc ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa
     9241 gaaagcgggg accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt
     9301 tgccgtcatc ggtctggagc tggcgggcgt agctgccggt atggcgggtt tactcccggc
     9361 tgaagggcaa acgccagact ccaaaaccat catcatctct attaccaccc tggcggtcac
     9421 ggttttaggt tccgtgctgt ttcgtggttt cctggcaatt atcccgattt taattggcgt
     9481 gctggtgggc tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa
     9541 tgctcactgg tttgcgctgc caaccctcta tacgccgcgc ttcgagtggt ttgccattct
     9601 gactattctg ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtagtaac
     9661 ggctaatatc gtcaaaaaag atctgctgcg cgatccaggt ctgcaccgtt cgatgtttgc
     9721 taacggcctg tcgaccgtga tctccggctt ctttggctct acgccaaata ccacttacgg
     9781 agaaaacatt ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggtggggc
     9841 ggcgattttc gctatcctgc tttcctgtgt cggtaaactg gctgccgcta tccagatgat
     9901 cccattgccg gtgatgggcg gcgtttcgct gctgctttat ggtgtcatcg gtgcttccgg
     9961 tattcgtgtt ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac
    10021 ttccgtgatt ttgatcatcg gcgtcagtgg cgcgaaggta aacatcggcg cggcggagtt
    10081 aaaaggtatg gcgctggcga ccatcgtcgg tatcggctta agtttgatct tcaaactgat
    10141 tagtgtgctg cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa
    10201 ataatgtacc gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa
    10261 acttctcgcg attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct
    10321 ctttgccact ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact
    10381 cctctttact ggctgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc
    10441 tctgggcacg cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt
    10501 cgcagcgtgg cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg
    10561 aagtgctcga cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg
    10621 caggcgatga gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg
    10681 gcaaaacacg attgttgatc accggcgatc gtccaccgcg gcagttgaat ctgggattac
    10741 cggatctcgc gtcgcgactc gactgggggc agatctacaa attgcagcca ctttctgatg
    10801 aagataagtt gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag
    10861 atgtggggcg tttcttgctg aagcggctcg acagagaaat gcgcacgcta tttatgacgt
    10921 tggatcagtt ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga
    10981 aagaaattct gaagttgtag ataaggtgtt tattgtcgga tgcggcgcga acgccttatc
    11041 cgaactacgc gaagttaatc catagtattc gtaggccgga taagacgtgg caagcgtcgc
    11101 atccggcaat gtgcttcaac tagcgatgga gcacatctta acccacgatt tccagtacct
    11161 gctccggtgg acgaccaatc cgcgctttgc cattcgcgac cacaatcggg cgctccatta
    11221 gcttcggatt atccaccatt gcctgaatca gcgcctcttc gctaagcgaa ctgtcagcca
    11281 ggttcagttc tttataaaga tcctctttct ggcgcatcaa ttcacgggcg ctattcatcc
    11341 ccagcatttt cagcaaatcg cgcagcgttg ccgcatcggc gggtgtctca aggtagagca
    11401 ccacttctgg ctccacgccg ttttctttca gcaggttcag cgtttcccgg ctctttgaac
    11461 agcgtgggtt atggtagatt tttacctgtt tggtcattgt tcttccttta atgcgaatta
    11521 catcttggta taaggcttaa agcgttcctg cagctggcgc aactggtcga tgcgcgcatc
    11581 gtaacgcgct tgttgcaggc tgcctaattt cacctgcgaa ctggcgctac tcaacagcga
    11641 aatggcctga tcgagtcgtc cggcgagcgc ataaccttct gctcgcgcag ccagctcctg
    11701 atcacggtta tttagcgcgg cttccgcctg tgccagcaaa tcccagccgt tgctgtcatc
    11761 tttattatta aaggtgtagc gattcagaat attggccgct tcttgtggtt gaccaccttg
    11821 cagataagcg ttcgccaggt tgagctgcaa caccggattg gtgcgtaaat cgcgggcatt
    11881 tttcaggcga ttgatcgcct cattggcttt gttttgccca agatcgatat cggtagccag
    11941 atcgagatac catgcgttgc caggttctgc cgccagtaac ggttgcagcg ttttacgcgc
    12001 ctcgtcgtat ttattggctt ccatcgcctg taaagcacga ccatattgtg ctgctcgttg
    12061 ctgacgaacg tttcctttcg cccattcatc cagcaaatca ctggtgagct ggttacgtcc
    12121 ggaattatac atccccagtg tgcgcgcttt cgccagatag aaatcttccg acgactgcac
    12181 caccatcggg cgcatctgat tagcacggtt gcgggcatct gccagacgac tttccggcaa
    12241 cgggtgagtc aataaaattt ccggcgggcg cgaggagtaa cgcgcctgat cgagtaattt
    12301 ttcgaggaag gtaggcatcg cctgcggatc gaatcccgag cgttgcagca cctgaatacc
    12361 aatgcggtcc gcttcctgtt cattttgctg ggtgaaactg atcatcccct gacgcgttcc
    12421 cgccagtgta ccggtcagcg ccgccatccc cgcctgcgga ctggccatcg ccagtaaaat
    12481 agaacctaac gcgccgaccc aggtcagcgg cgcgctgcgc tgctgatctt ccatcgctcg
    12541 cgccaggtga cgttgggtga cgtgggagat ttcgtgcgcc ataactgaag ccagttgact
    12601 ttcgttatcg gaataacgga acagggcaga gtgcagcacc acgttgccgc caaagaaagc
    12661 aaaggcgtta atttcgtcgt tgttgatcag aaagaaatga aacggtgtct taaccgaatt
    12721 ggcatgcgaa accagacgca tccccagcga attaatatat tgcgttaaca gcgggtcatt
    12781 aattaacggc gcgctgccgc gtagctggcg gacataatag tcgcccatct gcatttcctg
    12841 accaatggaa agcgtgcttc ctgcggaggt tcccatatcc ggcaaggtgt ctgcgctgtc
    12901 ggcaaacgcc ggggctacct gaccaatggt catagcagca atgagggttg caaccaggtt
    12961 ttttttcaac tgcctgaaca taacctctat cctgtatttc tgaagatagt catttgaccg
    13021 atactgtgaa atatcgttcc caccgcgccg cctttgagtg taacggcgaa gattagccca
    13081 ctcaaatgac ttttcagttt cctgaatccg tcataaaaag cgaagtcttt tttgtgacat
    13141 ttcgatacaa ttgcggatcg caatcccgct cttgaggttc agggaaggtc tttatgctcg
    13201 aaatgttgat gcaatggtat cgccgccgtt ttagcgaccc ggaagcgatt gccttgctgg
    13261 ttattttagt tgccggattt ggcattatct ttttctttag tggcctgctt gctccgttgc
    13321 tggtggctat tgtgctggcc tatttgctgg aatggccaac cgtgcgcctg caatctattg
    13381 gctgctcccg ccgctgggcg acgtcgattg tattggtggt ttttgtcggt atattgctac
    13441 tgatggcgtt cgtggtactg cctatcgcct ggcaacaggg catctactta atccgcgata
    13501 tgccggggat gctcaataag ctttctgact ttgccgccac gttgccgcgc cgctatccgg
    13561 cgttaatgga tgcgggcatt attgatgcaa tggccgaaaa tatgcgcagt cggatgctga
    13621 ccatgggcga ttcggtggtg aaaatttccc tcgcctcgct ggtcggtttg ctgaccatag
    13681 ccgtctatct ggtgctggtg ccattgatgg tcttcttcct gctgaaagac aaagagcaga
    13741 tgctgaacgc cgttcgtcgg gtgctgccgc gcaaccgtgg actggcagga caggtgtgga
    13801 aggagatgaa tcaacaaatc accaactata tccgcggcaa agtgctggag atgatcgtgg
    13861 tggggatcgc cacctggctg gggttcttgc tctttgggct gaactattcg ctgctgctgg
    13921 cggtgctggt cggcttctcg gtacttattc cgtacattgg cgcatttgtg gtgaccattc
    13981 cggtggttgg cgtggcgcta ttccagtttg gtgcaggcac ggaattctgg agctgcttcg
    14041 cggtgtatct gattattcag gcgctggacg gcaacctgtt agtaccggtg ttgttctccg
    14101 aagcggttaa cctgcatccg ctggtgatta ttttatcggt ggtgatcttc ggtggtttgt
    14161 ggggattctg gggcgtattc ttcgccattc cattggcgac gctgatcaaa gccgtgatcc
    14221 acgcctggcc cgatgggcaa atagcgcaag aataatcttc agcccgacga actgtttcgc
    14281 cgggctgatt tttcaggatt cctgacgtaa ataaatagcc cgataacaca tactcaccag
    14341 cactgcaccg ccgataatat tacccagcat caccgggagc agattggcgg taataaaatg
    14401 gctgaccgtc agtgcaggaa aattgtctgc gctactgtgc gccagttgcc gaaggggcgt
    14461 ggggcaaaat ggcgaatggc aatggcgaac ggaatcacaa acaaattggc gatacagtgc
    14521 tcaaagccac tggcgacaaa cagggtgatg ggcaaaatca tagcgacgat tttgtcgcat
    14581 aacgaacgcc cgcaataact catccacagc gccaggcaaa ccatcagatt gcacataatg
    14641 ccgaggctga cagattcagt aaatgtatga tgcattttgc cctcggcgca gtgtaaaacc
    14701 gcgactcccc acatcgcgtt ttcactcatc accagcccgg aaaaccagat taacaaactg
    14761 aaacaggcaa tacctgccag attaccgcag gccaccagaa gtgcgttaat cagccaagtt
    14821 cgccaactaa taacgccccg actttttgcc attaccgtca ttaccgacga ggtgaacagg
    14881 ttggtgccgc aaaccgccag caaaataaag ccaagtgtaa agcaaaggcc gcccaccaga
    14941 tgggttaatg cctgtgaaga cggggcatcg gcgataactg aaaggtaaaa agtaaatccg
    15001 atcgccataa acacgccagc ggacatcgcc attaaaaaga ccgacacttt actgcgggca
    15061 attttatggg aggcaatccg ttcagcgatt gccgcttttc tggcgctcaa ctgcaagtcg
    15121 aaagagagtt tgtttctcat gcatactcct gcaaaagcag attacaacac ctcgcgaacc
    15181 gagattccca gacgctgcat tcgtgacagc agcgtggtgc gcttcatccc taatcgtgtc
    15241 gccgcgccac ggggcccggc aacaatgcca ttggtttctc gcaatacctg aacaatgcgc
    15301 tgacgttctt cttcgtcatt ctctggcgtc gtcgggtgca gcaactgcgc cattgaactg
    15361 cgaagcgctg aatcttcatt tagcgtcggc agtaaacggc tttgtcggac attaagatgt
    15421 aaattcagac tgttaccacg agtcagcagt accgctcgct caatcacgtt ttccagctcg
    15481 cgcacgttgc ccggccagtc ccacgacatc aactggcgta gcgcctcggt cgggatggcg
    15541 tcaattgaac gattcatatg gcgcgccatt ttttgcgtga aatgctttgc taaaagaggg
    15601 atatcttccg gacggtcgcg cagcggcggc aattccagtg ggaagacatt caggcgataa
    15661 aagagatcgc tgcgaaactg gcgatcttca accatttgcc acaaatcacg gttggtggcg
    15721 gcaatgactc tgacatttac cgggatcgtt ctactcccgc cgagacgctc aatctcccgt
    15781 tcctgcaata cgcgcagcag tttaggctga agttctaacg gcagatcgcc aatttcatcg
    15841 agaaacaacg tgccgccatc ggcaatttca aaacgaccac gatgggtatt aatcgcacca
    15901 gtaaacgccc ctttgtcatg accgaataac tcactttcca gaagactggc ggggatggca
    15961 gcgcagttga ttttgaccag cggcttgtcg cgtcgcgggc taagttgatg gatcgctctg
    16021 gcgatcacct ctttgccggt tccggtttca ccgcaaatca acaccgtact gtcgctcttc
    16081 gccacaatat ctacctgctg gagtaggtct tccattgcct ggctttgata gataatgtca
    16141 ccgacgccca gattcgaaag gagctgctcg ctaagctggt ggttttcttg ctgcaaactt
    16201 tcctgcaaat cggtcatgct acgctaggca tcggcattgc caacggcaat agcgatgcga
    16261 tcggctatgt gttgtagtag ctggcagttt tcctcactaa agagagtgga agaggtatgc
    16321 gccagcaaca atgcacccgg tgtatggttg ccaaaggtaa gcggtataag gagcgcagat
    16381 tcgcagccgt tagatgcaag taagagtaat aacgggtcgc gttgccagag aaacagatca
    16441 tctgcacggt gcgtcaaggt cggttgtcgg gtttgtagcg tttgtgtcag caatacactg
    16501 tcgccaggca tattgcgtgg cagacacgca caatgtgagg cagaaagatc gctgcaccac
    16561 aggctgaact tctcgttctt tcgatgatcg cccagtacca tactgactga agccagaccg
    16621 aaaaaatgat ggatctcacg agcgacgtca gcgatcagat cgtcgagatc aagatgtgac
    16681 agcactgcat tagtgatatc caccagtacc cgatgatgat cgcgatcttt ctccagcaaa
    16741 cgcagcgcca cctgctgttc ttcatcattg agccatgatt gcagaaccgg ggtgataatg
    16801 cgtgccagcc agttaaacag tgtgcaatcc tggggagtaa aacggcagcc gtgggtgcga
    16861 ataaagcgta gctccgccat tccactactg ccgagaagga cggcgtgacg cgaataatcg
    16921 atcatctcgc cctgatactc tgctgttgcc gtcacatcgc tgccatcatc atggtagcgg
    16981 taatagacgt tgcttttgag aacatgcagt tcaataagtt gtaccgggct gaacggcggc
    17041 ggcagcggga tgaaggcgcg taataaagac gccttaccgt gtttctgtgc taaccgctcc
    17101 gcgagcattc cgtttaccgc ttcaattgtt attccttgcg gcggggcaaa catcgcctcg
    17161 tctgacatag ccatcaacag tccttattaa tgagaaatcc ttctcacgca atttttctgg
    17221 ccatcagata gatggccggt tcctgcacaa tttcatccaa cagatgaatg aattgaatgc
    17281 tacgcgcctg ccacacagac gttgcgtcat ctgccagtgg cgcgaatgcc cgggtcaaca
    17341 gggcggtgtg gctggcgtca ataacaatct caccaaaagt catcaccccc agcatcttac
    17401 gtcgggcttg ctcctcttcg acctgggcaa gccaatcttc gtattccgtc agtgggcagc
    17461 ggaaggcgac attcagacaa tcaatcacgc caacgtgatg gccgatagcc agcgagtaat
    17521 acattacctg agagctgtgt tccggcatct cgtcgctact ggcgacaaac ttttttcgca
    17581 gcgtccagaa aacaatttcc ccgcactctt cagtcatgta acccccggag tacgcgaaag
    17641 agttgctgaa cgatactatt gagacgtgga tcgtcggcat cgcgcaacca ggtgttaacc
    17701 cgatttcctg taggatcgtc gcttaaatgg cgcaggagcc gatcgcaaat ttctcgcccc
    17761 tgacgatagc ccgccagccg ccgcgcttct cgctcaatgg cgatacgctg tgatggcggg
    17821 atctgcggcc acaacggttg catagtcacc ccggtgggat cgcgataatc caccgcgtga
    17881 atcttctgtt gcagcaaacc gagcgccacg gcgaaaccgt gaatggtggc ggccggtgtt
    17941 ggcgggcagc cggggatcca aacatcaatg gggacaatgg tgtcgctgcc gccccagacg
    18001 ctgtagagat cgtggaaaat ccgccgccgg caccgcacgc gccgtaggaa acacagattt
    18061 tatgatcggg ggcagactca tacgcccgaa gtgcaggcat acgcatcgcc cgggtgactg
    18121 cgccagtaaa taacaaaata tcggcgtgac gcggtgatga aacaacctta atgccaaaac
    18181 gttctgcgtc gaatactggt gtaatggcgg caaaaatttc gatttcacag gcgttgcagc
    18241 caccgcaatc gacgcgataa acgtaggctg agcgacggat atcctgtagc aaatgccgct
    18301 tcatcttttg cgtttgctcg tccagcgtga tgggctggct gacatgttgt gtaagcactg
    18361 gactcatagc tgctccttag ccaccagcgg tacatctgta tcgtcgttga gcagcgtcgc
    18421 gcgttgtttg cactccgggc agacgctcgc ttgcgcccat aacatttcgc ggttttgtgg
    18481 cgcattttgt tgctgtgcca acaattcagc agccagtgcg acggtttttt gctgggcaaa
    18541 cgggcgttcg caacggctgc aacgttgtag atggaacgtc gcgcgggtat agagatcggc
    18601 tttattggtg acggtcagtt caaagttatt ggtaagctgg atggctctgg tcgggcacac
    18661 ttcttcacaa cgtccgcagt aaatacaacg ccccagatag agctgccagg tgcgcgaatt
    18721 ttgctggtcg tcggtctgga tagtcagcgc atttgccgga caagcacagg cgcaggcacc
    18781 gcaggcaata cattggctgg gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc
    18841 cagtggcgcg aagggatatt tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa
    18901 cttcagcatc ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc
    18961 gatctcttta tacggcacgg tttttgactg gcgcttgcgc acatctacca gcgtcacatg
    19021 gtcggtacag gagtagcagg gatcaaggct accgataatc agcggtgcgt cagaaacggt
    19081 attgccgcgc agcatgtaac gcaacaccgg ccagttggcg taggtggcgg cacggcagcg
    19141 ccagcggaac aatttttggt tatcaccgag catgctccag tgcacgtctt caccgcgtgg
    19201 cgcttcaaca aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag
    19261 tggggtatcc ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat caaacgtctc
    19321 tttgacacgg accatcacgc gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt
    19381 tggaatattg ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg cgatttgtcg
    19441 gtcgagaatg ccaatgccct gagtgcgttg ttccatattc ggcgtagcaa gcagcatctc
    19501 taccagctcc gacacgtcgg cgcgcatctc gcgcaccagt ttcagcgttt gcagacgttg
    19561 ctctttgaga atatcgcggc gaacaccacc aatcagattc agaccgtagg ttttacgcga
    19621 cccgatcagc aattccgcca tcgtcatcga cttttcccgc actcggaaaa attgcataaa
    19681 gccggtatca aaaccaacga aatggcagga gaggccaagg ttaagcaaat gactgtgtag
    19741 ccgttcgact tccagcagaa tcgagcgaat agtatgtgct cgttgcggca cctcaatccc
    19801 cagtgcattt tcaaccgaat tggtataggc cacactgtgg gcaaaaccgc aaatcccaca
    19861 cacgcggtcc gataagaagg tcacttcgtt gtagcccatc cgcgtttctg ccagtttctc
    19921 catgccgcga tggacataaa acaggcggta atcagcatcg acaatttgct cgccatccac
    19981 aaacaagcgg aagtgacccg gttcatcgga ggtgatatgc agcgggccga cagggatcac
    20041 ccgcgcatcg ctgttgccct cattgataaa cgcatacgtt tcggaatctg tcgtcggttc
    20101 agggcgcagt cgataatcca tcgcatcttt gcgcagcgga tgcatatctt ccggccagtc
    20161 atcgggcaac accaggcgac gctgatccgg caggccaacc ggaatcagcc cgtacatatc
    20221 gcgaatttct cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg acggaaactc
    20281 ccgactgtcg gcatcgacca gtgccttcac cacgatccag catttttcgg ccccttccat
    20341 tgaaagggca taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt tgccaaacag
    20401 gactggaagc cagccatcat gttgataata aagataatgt acaacgtcag gcagcagatt
    20461 gattttcacc gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca gcaccgcgcc
    20521 ggggaactgc gttttcaggg cggcgagaat cgcttcgcca cgatttgacg atgaattaac
    20581 gttcactgtg tttctccgaa acagatgcgg tgcctgaagg caaaaaatca tgccaggtgc
    20641 tacgttgtgc aggcaggtcg tgcgtccctg agaggactat agtggaagcg cccgccagga
    20701 tcctgatgac aggttgtgga atatgcgttc ccatcgccag catcatgacc agcagaatca
    20761 ccattggcga ggtggtcaac cagccgagat caccccggtt aacggcctgc ggcggtttcg
    20821 ccattaacac ccgcgcagcc atccgtacca ggcccgccag caccagcgtt aacagcaata
    20881 acagcaggac gataatcagc aggtgattac gtgccagtcc tgcggtaacg gtcataaatt
    20941 cgctaagaaa aatgttgaag ggcggcatcc ctgccagcgc cagcgcaccg ccgccaaaca
    21001 gcacggcggt aaatggcatg attttgagca tcccacagac gacgttgaga tcgcgcgtgc
    21061 cgtacttgag cagtacattg ccggaaccgc agaacagcag cgtttttgcc agactgtggt
    21121 ttaaggtgtg cagcagcgcg gcaaaaattc ccagcggccc gccaatgcct agcgccaccg
    21181 cgaccagccc catgttctcc acgctggagt acgccagcag acgcttaatg tcccgctgta
    21241 ccagaatgaa aaatgccgcc acggcaaccg acaacatgcc gaagatgagc aacaaccggt
    21301 tggggaaatc gctgccgatg gcttggcaaa taatgatgta atagcgaatc agcaccaaca
    21361 gcgcgcagtt cagcaatacg gcggagagca gggcgctgac cggactcggc gcttcactgt
    21421 gagcatccgg cagccaggcg tgcatgggaa atagcccggt tttggtacca aagccaatta
    21481 gcacaaacac aaaggccaac agcattaatg ttgggtcaag caaggacgat tgcttaagaa
    21541 cctcgctcca gaatatcgcc atttctgcct gcggcataac gctggcggcg ttggcgtata
    21601 ccagcacggt accgaacaga ccaaaagcga caccaacagt acaaataatg atgtacttcc
    21661 atgctgattc cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc gagcttaagg
    21721 tggtggcttc gatcgccgcc cacatcacaa tcaggttatt gctggtaaca accagcagca
    21781 tggtgaacaa aaacagatgg aagaaaccgt agtaatcgca cagtgtaacg ggtgaaagct
    21841 cgccgtgtgc cacttcatga cgcatgtagc caatcgagta aacaccggtg agaaagccaa
    21901 tcacaccaag aatggcgagg aacaaaccgc ccagaccatc aatatgaagc cacagtcccg
    21961 cagcgaatat ttctcctgca tcagcggcag tttggaccac ccagagtgcc agaatcagca
    22021 gcagtgtgat ccctaagcta tgtaatacag tcaccgtaca agtcgcagag agcccccgtt
    22081 tccggcaggc aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt aaagcgaaca
    22141 tcacagaata actcatctca ttatcccttc agcgcggtca agttgttcac gtccagcgtg
    22201 ccgtgggtac gccatatttt tcttgccagt aacaccatca caatgacggc gaagatggcg
    22261 tcggtagcga tacctatttc caccagttcc ggtgctcgcc aggcaagaag cgccagcacc
    22321 agatgggagc cgttttccat caggcagtaa ccaaaaattt gccgcaggat attgcgctgg
    22381 ctgacaatgc acagcaggcc aagcagaaaa tgacctaacg ctaccgccag cgccggtttc
    22441 agcccggtag ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag caggacaatt
    22501 aacgccgcga gcagtgccat cattgccgga ccgaataacg ctttttccgg gatgttctgg
    22561 ggaatatttc gtgcagcgta agtcatgatt aacggtacca gcagcacttt ggtgataaag
    22621 gcgctggcgg accagatcag cagttgctct gcggcgaaca ggcacgagag agtggcgaaa
    22681 atagacacca gcaccagcga ctggcaggcg taaaatccgc atgacaggcg atagcttttg
    22741 acgctaatca caaacagcga tgtcagcatc atcagtcccg ccagattatt tacgatcata
    22801 gaaccggtca ttcatcatct ccttactgca accaggtcgc ggcgattacg ctggaaatca
    22861 gcgacatcac aatcagtaca atcaacacca ggcgcattga ccctggcagc ggtgcggcat
    22921 cggcgacggc ctcgctcggt ttgccaggca caacgcgacc aaaccagcga ataaaccagg
    22981 cgaaactggc gaccgattca atcatcagca gaatcatggc gggcagcagg atccagtact
    23041 ccactgacaa cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag ccgttgaacg
    23101 gcggcacacc ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc aacggcaggg
    23161 tgtgcaatac gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac
    23221 ctgctacaag gaaaaacagg cttttagcga acgcgtggtt gacgatgtag gcgatgctgc
    23281 cctccagtgc cagccgccca ctttcacctt cgatgcggcg tggagatagg cactgatcgg
    23341 tgttggcgct tccattgcat ccggtagcca cgcttgcatc ggtagctggg ccgatttccc
    23401 ccacgcggca aacaggatgc cgccataaac cagataacgt gcgtcgccat gtaactcgct
    23461 catcgcgcta agcacacacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa
    23521 ccccaacgaa ccgatatggg tgataagcaa cgcttttagt gctgaacgct gcgctttatc
    23581 gctctggtaa taattgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa
    23641 ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa
    23701 aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata
    23761 acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt
    23821 actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa
    23881 cgtgacggca actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt
    23941 ggtcagcgcg gcgaacaaaa ccccccattc ggcggcccga cgttgtggcg aaaacgaaac
    24001 gaccagtgcg ccgataaaag gcagcaataa cgtcgtcaga gcaagatttt ccatattccg
    24061 tcagtgctcc ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc
    24121 caagccaggt cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga
    24181 ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca
    24241 ggcaggcgag agaaagttct tgcgcgcgcc caaaggacag aaacagggcc acaaacagtg
    24301 atgccatcac gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg
    24361 aatattcggt cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa
    24421 tttttcccat ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta
    24481 gcggcgagtt ccagcccatc gccagcgtat tgctgatcat ctcgatatgc gtggaacctg
    24541 ctatcagcgc caataccagc agtgagagaa taagcattgg ttcgaccaga atgccgagcg
    24601 tcaactcgcg actggcaccg actcccgcaa acggacttcc ggtatccagc ccggaaagag
    24661 caaagaaaaa acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg
    24721 caaaagggga aacggtaata aacagtggta aggccatcgc cagcaccagc atgctgctga
    24781 ttaataccca cggcatcagg cggaacatca gacctgaaga tgtcggcgca acttcctggc
    24841 gtttaaacag tttgtggata tcgcgataat cctgccagat ccccggcccg cggcgggagt
    24901 gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag tggggttagc atcagcagaa
    24961 tcacggcctg tgctaacgca aaaataacca gatatccgtc gcaaagagtt tgtctcatgg
    25021 tgatttcctt agacggcaat agcgattagc agcacaacca gtgcggcgac cacatacagg
    25081 caatagagac gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg
    25141 gcgctcacga tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg
    25201 gcggtgacgt gcgcaaaacc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga
    25261 cgaaataacg cagagaagac cacacgcagc ggctgcatca cgccattgcc tgacggggcc
    25321 atcgcatttt cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg
    25381 cggcgcgaac gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac
    25441 agcaacagta atgagggggt gacctgtgta tgaaacgtgc cgggtacaag tgctattccg
    25501 ctggcgacag tgacgggagg ggtattggta aacgcatggg caatatgcat tatcttcggt
    25561 gccagccaac tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg
    25621 acgatcatcg tatttggcac ttcctgtgcc tcttcagcgt gtgtactgcg cggcgcacca
    25681 cagaaagtaa taccgtacat ttttacgaag cacattactg ccagcccacc agtgactgcc
    25741 agcattacca tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt
    25801 gagaacagcg actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct
    25861 gatatcgcga ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt
    25921 gcccccattt tttccatgtc gtgggtatgc aaacgcgaaa taatcgcgcc cgcgccgaga
    25981 aatagcagcc ctttgaacag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca
    26041 accacggtga gcagcgggtc gtgcagtgac agaccgacca tcgccacacc gactgccagc
    26101 aaaataatgc cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc
    26161 gccagcgcat acagcacgcc caggagcgcg gagattgcgc cgatcgccat caccagaatg
    26221 ccccaccaca gcggcaaacc cgtttgcgcc agcaggtcca tcgctacttt caggatgccg
    26281 aaaataccga ttttgaccat tacgccagac atcaacgccg aagcgtgcga tggtgcggca
    26341 gggtgagcgc gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg
    26401 aaaaaggcca gcaggaacac cgccgacgcc agccccggag aaagtgaaag cgtgcggaaa
    26461 ctggcaaaat cgaggctgcc gctttcgcgc cccatcagca agaaggcgat cataatcagc
    26521 accgatccgg cgtgggcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc
    26581 tgcctggcaa tgaccagaaa cctggaagac agcgacatca tttcaaacag cacgatgaac
    26641 caaaaagcgt tgtccatcac cagcagggca accatcgatg cgatgaaaag attcataaag
    26701 aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagagc
    26761 gaacaaacca ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt
    26821 cgcagggtta aatcggcaaa gtcaaaaggg gtggcaaaat gggcgactaa agttacacca
    26881 gcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg
    26941 ccggaaagct taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca
    27001 aacagataga ggatcagcga ccaggttaat aattgcaggg catccattag tgttgctcct
    27061 gagtgaggga aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt
    27121 tttctttcat ctgacgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc
    27181 aagcgcgaac gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga
    27241 cggtctggac acccggttcc cagcgcagca acgcatgttg gggcagcacg ttttcttcgc
    27301 cgtctttgag tgagccttca gcctgaaaaa catattgcgc atgggcattc accggacggc
    27361 ttcctgaagc gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac
    27421 agaggctttc gttgagttgg atcgcatcat ccctctgaga gatggcattg accgggcaga
    27481 cctgcaggca aggggcttcc tcacagtgat gacacacgac aggggcagtg attgttgacg
    27541 tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac
    27601 aggcagcgag acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt
    27661 tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgcc
    27721 attcttatta actcaatata ttcaataagt tatgtattga ctggattctg aacggtacaa
    27781 aaatgatggt cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag
    27841 cgagaatgtg atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg
    27901 gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat
    27961 tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc
    28021 tgatgcgatg aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga
    28081 attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt
    28141 ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg
    28201 cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg
    28261 tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa
    28321 cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg
    28381 gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact
    28441 gccttgtgca ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc
    28501 cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc
    28561 agcaggttgc gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc
    28621 tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc
    28681 tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc
    28741 ggcacctttc aacggtaacg ttgattcaat cagagtaatg gcattccatg aaccggaaag
    28801 cagcataata aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca
    28861 gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa
    28921 cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg
    28981 tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt
    29041 ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg
    29101 gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt
    29161 ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat
    29221 cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt
    29281 ggtgatgatg acgctggagc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc
    29341 taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg
    29401 ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt
    29461 caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac
    29521 tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg
    29581 aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga
    29641 tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg
    29701 tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa
    29761 actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt
    29821 acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact
    29881 gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg
    29941 tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga
    30001 tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat
    30061 tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct
    30121 acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga
    30181 cctccggtga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg
    30241 acattcgtcc accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg
    30301 gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg
    30361 ttagtgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac
    30421 tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt
    30481 atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc
    30541 tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga
    30601 tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc
    30661 aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccga
    30721 tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa
    30781 aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg taacatggcc gtaacttata
    30841 agccgctgtc tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg
    30901 gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc
    30961 cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg
    31021 cgtttagcaa gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg
    31081 caaacgtgtg cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg
    31141 tcggacgtag ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga
    31201 cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc
    31261 tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt
    31321 gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg
    31381 caaaggtatg gtgaacaaca agttcaacta cttcatcatg agcaaactgg ctgaagcggg
    31441 tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga
    31501 tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct
    31561 tggaatcgaa gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga
    31621 cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa
    31681 agagaacctg gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact
    31741 gttcgatgat gctggtctga ttctggtcga cttcaagctg gagtttggcc tgtacaaagg
    31801 tgaagtggta ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac
    31861 actggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta
    31921 tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgtttc
    31981 tttagccgga gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt
    32041 atctcccttt gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca
    32101 aaaagggggg gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt
    32161 accgaaagcg gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg
    32221 tacaagaatc atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt
    32281 gagtgcggat tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg
    32341 atgctgaaat tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta
    32401 catgtcagag agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg
    32461 atggagctta tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt
    32521 gaaggtttcg tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc
    32581 gggcaaatca gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg
    32641 atagcgggac atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt
    32701 gagcgagtgg cgggaatcag actagaagac tggagctaac attctctttt acttctaaca
    32761 ttcgtaaatt atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc
    32821 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
    32881 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
    32941 ccggtggtcc gtctatgggc ggtcctggtt ttcgcctgcc aagcggtaaa ggcgggctga
    33001 ttttactgtt agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
    33061 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
    33121 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
    33181 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
    33241 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
    33301 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
    33361 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
    33421 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
    33481 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc
    33541 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
    33601 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg
    33661 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
    33721 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
    33781 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
    33841 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
    33901 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
    33961 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
    34021 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
    34081 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg
    34141 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
    34201 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
    34261 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
    34321 gctactgacc atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
    34381 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
    34441 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc
    34501 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
    34561 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
    34621 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
    34681 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
    34741 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
    34801 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
    34861 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
    34921 ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc
    34981 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
    35041 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
    35101 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
    35161 gcgtcagcgg gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga
    35221 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg
    35281 ctgcggtttt gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac
    35341 ggcgatggcg ctgttaccga tgagtgatgc gggtaaacag ctggctgaac gtgagcatta
    35401 ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacac ttcctgttga
    35461 tccactaaac gatatcgtgc tttctgacga cgactggctt gaactggccg gttttgcttt
    35521 tgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac aaaccagtga
    35581 actggcatta ccggcgctgc gtgggcgttt acagaaaaat gccagtgatg cgcagttatg
    35641 taccacactt aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc
    35701 gcaggcgctg tgcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
    35761 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg
    35821 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
    35881 gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg
    35941 tggcaatggg ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga
    36001 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg
    36061 gtattacgga agataatcgc caggcgcgtg tggatgcgat catgccgacg tttattgaaa
    36121 cggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacggca ctcatcggtt
    36181 tctctcaagg cgcgattatg gtgctggaga gcattaaagc cgaaccgggc cttgcttcac
    36241 gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct acagccacga
    36301 caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg
    36361 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
    36421 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
    36481 cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg
    36541 aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg catcaggcat
    36601 cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc
    36661 ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa
    36721 atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt
    36781 gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca
    36841 atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag
    36901 ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca
    36961 ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat
    37021 gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc
    37081 attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa
    37141 tggctgcccg gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc
    37201 aagcacctgt gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg
    37261 cacaaacagt tcacccggaa taacattgtt actgcccgtt cccgcctgaa tattagcaat
    37321 ctgcatactg gtcgccggga aaaattcatt gccctgatcc cactcaatag ccactaattc
    37381 attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgcgggt aggccacatg
    37441 cccctgaacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac catttttcac
    37501 cacatcacca accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc
    37561 attacgtgcc atcaacgctt cgacgacttt taccgtaccg ttgtgggcac tggcttcttc
    37621 atcagaggtg atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg
    37681 ttctgccgct accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgtccgaa
    37741 taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc
    37801 aggcggcacc acgtcggtat gcccggcaaa ggccaacgtt tcaccttgcc cacgccatgc
    37861 ccaaaaattc tgcgtatcgg caaaatccat gcgttcaacg gtaaaaccga tcgcctgcaa
    37921 acgttcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat
    37981 aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg
    38041 ctgataactg gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg
    38101 acgtttgata attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat
    38161 tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc
    38221 ttcccagcct aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg
    38281 gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat
    38341 ggtgtcacaa tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatttatga
    38401 gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc aggaaatata
    38461 acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
//
LOCUS       DAFAVF010000026        37990 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFAVF010000026 DAFAVF010000000
VERSION     DAFAVF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.026"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            165..836
                     /locus_tag="LGK42_001330"
     CDS             165..836
                     /locus_tag="LGK42_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBK2657593.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            846..2042
                     /locus_tag="LGK42_001331"
     CDS             846..2042
                     /locus_tag="LGK42_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK2657594.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2020..2601
                     /locus_tag="LGK42_001332"
     CDS             2020..2601
                     /locus_tag="LGK42_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK2657595.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2603..3376
                     /locus_tag="LGK42_001333"
     CDS             2603..3376
                     /locus_tag="LGK42_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBK2657596.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3561..3836
                     /gene="frmR"
                     /locus_tag="LGK42_001334"
     CDS             3561..3836
                     /gene="frmR"
                     /locus_tag="LGK42_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBK2657597.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3871..4980
                     /gene="frmA"
                     /locus_tag="LGK42_001335"
     CDS             3871..4980
                     /gene="frmA"
                     /locus_tag="LGK42_001335"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBK2657598.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5073..5906
                     /gene="fghA"
                     /locus_tag="LGK42_001336"
     CDS             5073..5906
                     /gene="fghA"
                     /locus_tag="LGK42_001336"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBK2657599.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6031..6570)
                     /locus_tag="LGK42_001337"
     CDS             complement(6031..6570)
                     /locus_tag="LGK42_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBK2657600.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6672..7883)
                     /gene="mhpT"
                     /locus_tag="LGK42_001338"
     CDS             complement(6672..7883)
                     /gene="mhpT"
                     /locus_tag="LGK42_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBK2657601.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8059..9072)
                     /gene="dmpG"
                     /locus_tag="LGK42_001339"
     CDS             complement(8059..9072)
                     /gene="dmpG"
                     /locus_tag="LGK42_001339"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBK2657602.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9069..10019)
                     /gene="mhpF"
                     /locus_tag="LGK42_001340"
     CDS             complement(9069..10019)
                     /gene="mhpF"
                     /locus_tag="LGK42_001340"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBK2657603.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10016..10825)
                     /gene="mhpD"
                     /locus_tag="LGK42_001341"
     CDS             complement(10016..10825)
                     /gene="mhpD"
                     /locus_tag="LGK42_001341"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBK2657604.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10835..11701)
                     /gene="mhpC"
                     /locus_tag="LGK42_001342"
     CDS             complement(10835..11701)
                     /gene="mhpC"
                     /locus_tag="LGK42_001342"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBK2657605.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11719..12663)
                     /gene="mhpB"
                     /locus_tag="LGK42_001343"
     CDS             complement(11719..12663)
                     /gene="mhpB"
                     /locus_tag="LGK42_001343"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBK2657606.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12665..14329)
                     /locus_tag="LGK42_001344"
     CDS             complement(12665..14329)
                     /locus_tag="LGK42_001344"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBK2657607.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14407..15354
                     /gene="mhpR"
                     /locus_tag="LGK42_001345"
     CDS             14407..15354
                     /gene="mhpR"
                     /locus_tag="LGK42_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBK2657608.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15431..16513
                     /gene="lacI"
                     /locus_tag="LGK42_001346"
     CDS             15431..16513
                     /gene="lacI"
                     /locus_tag="LGK42_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBK2657609.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16636..19710
                     /gene="lacZ"
                     /locus_tag="LGK42_001347"
     CDS             16636..19710
                     /gene="lacZ"
                     /locus_tag="LGK42_001347"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBK2657610.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKVAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19762..21015
                     /gene="lacY"
                     /locus_tag="LGK42_001348"
     CDS             19762..21015
                     /gene="lacY"
                     /locus_tag="LGK42_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBK2657611.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21081..21692
                     /gene="lacA"
                     /locus_tag="LGK42_001349"
     CDS             21081..21692
                     /gene="lacA"
                     /locus_tag="LGK42_001349"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBK2657612.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(21795..22976)
                     /gene="cynX"
                     /locus_tag="LGK42_001350"
     CDS             complement(21795..22976)
                     /gene="cynX"
                     /locus_tag="LGK42_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HBK2657613.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPQVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22982..23452)
                     /gene="cynS"
                     /locus_tag="LGK42_001351"
     CDS             complement(22982..23452)
                     /gene="cynS"
                     /locus_tag="LGK42_001351"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HBK2657614.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23483..24142)
                     /gene="cynT"
                     /locus_tag="LGK42_001352"
     CDS             complement(23483..24142)
                     /gene="cynT"
                     /locus_tag="LGK42_001352"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HBK2657615.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24252..25151
                     /gene="cynR"
                     /locus_tag="LGK42_001353"
     CDS             24252..25151
                     /gene="cynR"
                     /locus_tag="LGK42_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HBK2657616.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25191..26474)
                     /gene="codA"
                     /locus_tag="LGK42_001354"
     CDS             complement(25191..26474)
                     /gene="codA"
                     /locus_tag="LGK42_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HBK2657617.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26464..27723)
                     /gene="codB"
                     /locus_tag="LGK42_001355"
     CDS             complement(26464..27723)
                     /gene="codB"
                     /locus_tag="LGK42_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBK2657618.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27960..29846)
                     /gene="prpE"
                     /locus_tag="LGK42_001356"
     CDS             complement(27960..29846)
                     /gene="prpE"
                     /locus_tag="LGK42_001356"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBK2657619.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29886..31337)
                     /gene="prpD"
                     /locus_tag="LGK42_001357"
     CDS             complement(29886..31337)
                     /gene="prpD"
                     /locus_tag="LGK42_001357"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBK2657620.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31371..32540)
                     /gene="prpC"
                     /locus_tag="LGK42_001358"
     CDS             complement(31371..32540)
                     /gene="prpC"
                     /locus_tag="LGK42_001358"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBK2657621.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32585..33475)
                     /gene="prpB"
                     /locus_tag="LGK42_001359"
     CDS             complement(32585..33475)
                     /gene="prpB"
                     /locus_tag="LGK42_001359"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBK2657622.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33714..35300
                     /gene="prpR"
                     /locus_tag="LGK42_001360"
     CDS             33714..35300
                     /gene="prpR"
                     /locus_tag="LGK42_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBK2657623.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35401..35676)
                     /locus_tag="LGK42_001361"
     CDS             complement(35401..35676)
                     /locus_tag="LGK42_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2657624.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35823..36494
                     /locus_tag="LGK42_001362"
     CDS             35823..36494
                     /locus_tag="LGK42_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBK2657625.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36511..36756)
                     /locus_tag="LGK42_001363"
     CDS             complement(36511..36756)
                     /locus_tag="LGK42_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657626.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37169..37984)
                     /locus_tag="LGK42_001364"
     CDS             complement(37169..37984)
                     /locus_tag="LGK42_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657627.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT         8974 a        10238 c         9843 g         8935 t
ORIGIN      
        1 gcataaaaac attacccagt cgaacctccg ggtctgaaag cccttgcttt tcagttatta
       61 gcaacattta acatttataa attaacatct ggctgcgcca gagattacgt ctctgagtgc
      121 atcgttgtgc ttgtagtata ataaataaaa tggagtgtca ttaagtggat aaggttttag
      181 attcagccct cctttcttcg gcaaataaaa gaaagggtat ccttgctatt ggcgcacatc
      241 ctgacgatat agaattaggc tgtggcgcat cgcttgctcg tcttgcgcaa aaaggaattt
      301 atatcgcagc agtggtgatg actaccggca actctggtac agatggaata atcgatcgcc
      361 atgaagaatc gcgcaacgcc ctaaagatat tagggtgcca ccaaactatt catcttaatt
      421 ttgctgacac ccgcgctcat ttacagctca atgatatgat ttccgccctg gaagacatca
      481 ttaaaaatca aattccttct gatgttgaaa tcatgcgggt atataccatg catgatgccg
      541 accgccatca ggatcatctc gctgtttatc aagcttcaat ggttgcctgc cgcactattc
      601 cacaaattct cggctacgaa accccaagta cctggctttc atttatgcct caggtttttg
      661 aatccgttaa agaagaatat ttcacagtca aacttgctgc attaaaaaaa cataaaagcc
      721 aggaacgacg tgattatatg cgtcatgatc gcctgcgtgc agttgcacaa tttcgcgggc
      781 aacaggtcaa tagcgatctg ggtgaaggct ttgtaattca taaaatgatt ctttgagcaa
      841 attgcatgaa aacctggata tttatctgta tgtccatagc aatgttgcta tggtttttaa
      901 gtacgctaag acgtaaaccc agtcaaaaga aaggctgtat tgacgccatt atacctgcgt
      961 ataacgaagg cccgtgtctg gcgcagtcac tggataatct actgcgtaac ccttattttt
     1021 gccgggtaat ttgcgttaac gacggctcca cggacaatac cgaagcggtc atggcggaag
     1081 tcaaacgcaa atggggcgac cgctttgttg ccgtcacgca aaaaaatact ggtaaaggtg
     1141 gtgcgctgat gaatggcctc aattatgcca cctgcgacca ggttttttta agtgatgccg
     1201 acacctatgt tccgcccgat caagacggaa tgggctatat gctggcagaa attgagcgcg
     1261 gtgctgatgc cgtaggcggc attccctcta ctgcgttgaa aggcgcgggg ctgttaccgc
     1321 acatccgcgc gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta cagcagctcc
     1381 tgggcggcgc accgtttatt atcagcggtg cctgcgggat gttccgtact gatgtattgc
     1441 gtaagttcgg tttctcggat cgtactaaag tcgaagacct tgatctcacc tggacattag
     1501 tggcaaacgg ctaccgtatt cggcaggcga atcgctgcat cgtataccca caggaatgca
     1561 acagtccgcg tgaggagtgg cgtcgctggc ggcgttggat tgtgggatac gcggtctgta
     1621 tgcgcctgca taaaagactt ttatttagcc gcttcggtat cttcagtata tttcctatgc
     1681 tgttggttgt gatttatggc gttgggattt atctcactac ctggtttaat gaattcatca
     1741 ccaccgggcc gcatggtgtg gtgttggcaa tgtttccgct tatctggatc ggcgtagttt
     1801 gtgttattgg tgcttttagc gcctggtttc atcgttgctg gttgttggtg cctttagcgc
     1861 cgctttccgt tgtgtatgta ttattagctt atgccatctg gattatttat ggacttattg
     1921 ccttttttac tggacgcgaa cctcagcgcg acaaacccac ccgctattcc gcactggtgg
     1981 aagcgtcaac cgcttattcc caaccttctg tcacaggaac tgaaaaacta tctgaagctt
     2041 aatgttaaag agaaaaatat tcagattgca gaccaggtaa ttattgatga aactgcaggt
     2101 gaagttgtta tcggcgcgaa tacccgtatt tgtcatggtg ccgttattca gggtccggta
     2161 gtgattggcg caaactgcct gataggtaat tatgcgttta ttcgtcctgg cacaataatc
     2221 agcaatggtg taaaaattgg ttttgccacc gaaattaaaa atgcggttat tgaagcggaa
     2281 gcaacgattg gtccgcaatg ttttattgcc gactcggtag ttgcaaacca ggcatatttg
     2341 ggcgcacaag tacgtaccag taatcatcgt ctggatgaac agcctgtgtc tgttcgaact
     2401 ccagagggaa ttatcgctac cggatgcgat aaattaggtt gttatatcgg gcagcgttca
     2461 cgccttggcg tacaagttat aattttgcct gggcgaatta tttccccgaa cacgcaactt
     2521 ggcccgcgcg tgattgtaga acgtaattta cctgccggaa cttattcact ccgacaagaa
     2581 cttatccgta caggagatta aaatgataaa acggacgtta ttagcggcgg ccatttttag
     2641 cgcattgccc gcttatgccg ggttaacttc cattaccgcg ggctacgatt ttaccgatta
     2701 ttctggcgat catggcaacc gtaatttagc gtatgctgaa ctggtggcga aagttgaaaa
     2761 cgcaacgctg ctttttaatc tttcccaggg gcgtcgtgat tatgaaactg aaaatttcaa
     2821 tgccactcgc ggtcagggtg cggtctggta taaatggaat aactggctga caacccgaac
     2881 gggtattgcc tttgcggata atacgccggt ctttgcccgc caggattttc gtcaggatat
     2941 taacctggcc ctgttgccaa aaacgctttt cacgaccggt tatcgctaca ctaaatatta
     3001 cgatgatgtc gaagtcgatg cctggcaagg cggcgtatca ctctatactg gcccggtgat
     3061 caccagctac cgctataccc attatgactc cagcgatgca ggtggtagtt atagcaatat
     3121 gatttccgtg cgtctgaatg acccgcgcgg cactggttat acgcaactat ggctaagccg
     3181 cggaacaggc gcttacacct atgactggac gccagaaaca cgctacggca gcatgaagag
     3241 cgtcagtttg caacgtattc aaccgctgac tgagcaactt aatctcgggc tgacggcagg
     3301 taaagtgtgg tacgacaccc caaccgatga ttataacggt ctgcaacttg cagcccatct
     3361 gacctggaaa ttctgattcc ttctgccgcc cgctatccgg ggcggccttc cctgccgatt
     3421 agccccccct ttcctctttg ttttccgacc acattcaccg gataaatttt attctccagt
     3481 gttatatact ataggggggt atgcattgac atatagcata cccccctata gtatattgcg
     3541 tgcagatgat gaggtgcgaa atgcccagta ctccggaaga gaagaaaaag gtccttactc
     3601 gagttcgtcg tattcgaggg cagattgatg ctctggaacg gtcgctggag ggtgatgccg
     3661 aatgccgtgc catactccaa cagatcgctg ccgttcgggg cgcggctaac gggctgatgg
     3721 cagaagtgct tgaaagccat atccgggaaa cgtttgaccg aaatgactgc tacagccgcg
     3781 aagtcagcca atccgttgac gacactattg aactggttcg agcctatctt aaatagctga
     3841 atctattacc atattgagga agagcgagag atgaaatcac gtgctgccgt tgcatttgct
     3901 cccggtaaac cgctggaaat cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg
     3961 ctgattaaag tcacccatac cggcgtttgc cataccgacg catttaccct ctccggggat
     4021 gacccggaag gtgtattccc ggtggttctc ggtcacgaag gggccggcgt tgtggttgaa
     4081 gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg tgatcccgct ttacaccgcg
     4141 gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta acctctgtgt tgcggttcgc
     4201 gaaacccagg gtaaaggctt gatgccagac ggcaccaccc gtttttctta caacgggcag
     4261 ccgctttatc actacatggg gtgctctaca ttcagtgaat acaccgtggt cgcggaagtg
     4321 tctctggcca aaattaatcc agaagcaaac catgaacacg tctgtctgct gggctgtggc
     4381 gtgaccaccg gtattggcgc ggtgcacaac acagctaaag tccagccagg tgattctgtt
     4441 gccgtgtttg gtcttggcgc gattggtctg gcagtggttc agggcgcacg tcaggcgaaa
     4501 gcgggacgga ttatcgctat cgataccaac ccgaagaaat tcgatctggc gcgtcgcttc
     4561 ggtgctaccg actgcattaa cccgaatgac tacgacaaac cgattaaaga tgtcctgctg
     4621 gatatcaaca aatggggtat cgaccatacc tttgaatgca tcggtaacgt caacgtgatg
     4681 cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt cggtgatcat cggggtggca
     4741 ggttctggtc aggaaatctc cacccgtcca ttccagttgg tcactggtcg cgtatggaaa
     4801 ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg
     4861 atgaaaggtg atatcgatct ggaaccgttt gtcacgcata ccatgagcct tgatgaaatt
     4921 aatgacgcct tcgacctgat gcatgaaggc aaatccattc gaaccgtaat tcgttactga
     4981 tttcccgcag gtataccccg tccacttcag acggggttct taatactctc cccgggcagc
     5041 cgtccggggg attaaccgtg agataatgac tgatggaact cattgaaaaa catgccagct
     5101 ttggcggctg gcaaaatgtg tatcggcatt attcccaatc actgaaatgt gaaatgaatg
     5161 tcggcgtcta tctcccacca aaagccgcga atgaaaaatt gccggtgctg tactggcttt
     5221 cgggcctgac ctgcaacgag cagaatttca ttactaaatc ggggatgcag cgttacgcgg
     5281 ctgagcacaa cattattgtt gttgcgccgg acaccagtcc gcgaggcagt catgtcgcag
     5341 atgccgaccg ttacgatctc gggcaaggtg ccgggtttta cctgaacgca acgcaagcgc
     5401 cgtggaatga acattacaaa atgtatgact atatccgcaa cgagctgccg gatttagtga
     5461 tgcaacattt tccggcaacg accagaaagt ctatctctgg tcattctatg ggcgggctgg
     5521 gcgcgctggt gctggcgtta cgtaacccag atgaatatgt cagcgtctcg gcgttttcgc
     5581 ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc ctttgccgca tatcttggtg
     5641 aaaataaaga tgcctggttg gattacgatc cggtgagtct tatttcacaa ggtcaacgcg
     5701 ttgcggaaat catggttgat caggggttga gtgatgattt ttacgcggaa cagctgcgga
     5761 ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa gacgttaatc cgttatcacg
     5821 agggttacga ccacagctat tattttgtct ccagttttat tggcgagcat attgcctacc
     5881 acgccaataa actgaatatg cgttgataat agtgcacgac tgccggatgc ggcgtgaacg
     5941 ccttatccgc ctacatttcg cccgcaggcc gtaggcatga taagacgcgc atagcgtcgc
     6001 atcaggcatc tgcgcacgac ttatctatgt ttaccacatc aaatcatcag gcactttgaa
     6061 gtcggcatac ggatcgtctt catcctgctc ttcggcactg agcgcgctgt gcaacactat
     6121 actgctggca tcacgctgag ctattttatc ggcaacgctg gcgggaataa tggcgtattc
     6181 accttcgcta ttgttatcga ccaacaagcg agcaatcgcc agacgaccat taatcaactg
     6241 cgcttgtgtg agcttatcga caaaaatctt cttaatcaga ttgccgtcag tgaagttaaa
     6301 accaatatcg ccattggcaa tggtgattcg gttcatttca atgagctgct tcacctgagc
     6361 tttatattct ttcgccaacg ccgcttgttt ttgctgttcg ctaagctgtt tatcacgctc
     6421 aagctgtgcc tttttatttt cttctaccgc cgcccgagct tcacgcgcct gaacgcgcga
     6481 ttttttcgcc gttctctcga ccttcgccgc ttttttgctg gtgactaatc ctgctttgag
     6541 caactgctct tgtaaggtaa gttttgccat ctttgtttcc aaacccgctg aataattttt
     6601 agcgagtata cctgtaaacg ccgtgggtgt attgggggca agtccctttc gcccctgctt
     6661 aataaggcgc gtcaggcatc ggcacacggc tgcattcttg atctccgact catcaaaata
     6721 aacaccgcca acccagcaac aagaataccc ggtgcagaag cggccattac gccgaccgtg
     6781 ccagtgccta atgccagcat tttcccggcc agtaacggac cgctcatagc ccccagacgc
     6841 cctaccgcca cggctgttcc cacacctgtt gcgcggatct gcgaactgta aaacaacggt
     6901 gccagggcat acaaaacgct ttgtccacct gtcgcaaaca accccgcgac aaatcccgcc
     6961 agcaacatac cgttaaatga cgacaccgtt ccaagcgcca gcagcgaagc taacatgccg
     7021 ctataaatca gtagcgacat ggttactgga cgcagcttat ccatcaatgc gcccaacatt
     7081 aacgtcccgc ttgccgcccc catttgcagg gcaaacatca cccctgccgc ctgcgatggc
     7141 tggaatcctt gctccaccaa aagtagcggt agccagttga tcaacatata aaccaccagc
     7201 agagtgaaga aataacacaa ccacagcagc agcgtcgcgg ttgccgtttc tggcgcaaat
     7261 aaggcacgca gtggtggcgc agcctgtttt tcgccagcga aaaccgctga ctccggcagc
     7321 cagcgcatta atagcggcac cagaatcaac ggcaccacac cacctaccca aaacaccgtt
     7381 tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg ccagcgccgc gccaatggga
     7441 acaccgcaat acatcaggct cactgccgtc ccacgaaaac gtggacccgc ggcttcagac
     7501 gtcagggcga taagattcgg caacgccgcc cccagcccga caccggtcat cagccgcgca
     7561 aagaccagtg aggggaaatc ccaggcaatc gccgttgcca gtgagaacaa accaaacagc
     7621 gcaactgagc caatcaaaat acgcttacga ccataacggt ctgccagcat cccgccaacc
     7681 aacgcgccgg gtagcaaacc gagtattccg gcgctaaata tccagcccat ttgcatttta
     7741 tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc cagccgcctg aagatccagc
     7801 ccttccatca gagcgaccaa aaaacaaagc ccgatggtca gcatcaggcg ggatgaagat
     7861 gatgaagggg tacgagtcga cataagatga acctcattgc gagtgcaggt gaatcgcgac
     7921 tgatacattg ccagccgcgt aatcagaact atttaatgac tgcctacggg cgaaatgtag
     7981 gccggataag gcgttcacgc cgcatccggc agtcgtgctg ggatgggcat acaaaatgca
     8041 cgttctcggc agtcatcatt atttgttgtt gcgcagatcc agcgccacgt caacgatcat
     8101 atcctcctgg ccgccaacca tccggcgttt gcccagctca acgagaatat ccaccgcact
     8161 taagccataa cgcgccgccg ccgtttcaca gtgacgcagg aagctcgagt aaacaccagc
     8221 gtatcccagc gccagcgttt cgcgatcgac tcgtaccggt cgatcctgca acggacgcac
     8281 caggtcgtcg gcggcatcca ttaacgcata gagatcggtc ccatgctgcc agcccagttt
     8341 atccgcggcg gcaataaaca cttccagcgg tgcgttacct gcgcccgcgc ccattcccgc
     8401 gaggctggcg tcgattcggt cgcagccctc ttccaccgcc gcgatagagt tcgccacgcc
     8461 aagactcagg ttatggtgag cgtgcatgcc agtttgcgtt tctggtttca gctctgcttt
     8521 cagggcgcgg aaacggtcac ggatatcgct catgttcatc gcaccgccag aatccaccac
     8581 ataaatacag gtcgcaccgt agccttccat cagctttgcc tgctttgcga gattctccgg
     8641 cgtggtcata tggctcatca tcagaaaacc aacggtgtcc attccgagct cgcgggcata
     8701 ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc gttgccacac gaaccacccg
     8761 cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg ccgattcctg gcagcaacaa
     8821 cgtcgcgatt ttggcgtgct tcaccacatc cgccgccgct tcaatccatt caaggtcgct
     8881 atgtgcgccg aaaccatagt taaagctgga accttgcaaa ccgtcgccgt gggccacttc
     8941 aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc tggcgaacgt tttccagcga
     9001 atactgatga cgaatggcgt gcataccgtc acgcaatgtg acgtccgaga tataaagttt
     9061 tttaccgttc atgccgcttc tcctgccttg cgcgccagtg actgggccat tttttccgct
     9121 gtcgccagcg cactggaagt cataatgtcg aggttgcccg cataggcagg cagataatgc
     9181 gctgcgcctt cgacttccag ccagaccgcc gttttcagcc cggagaactg acccacgccc
     9241 ggcaaattaa ccggtttatc ctgcgggata acttcaaact gcacgcgctg tttcaggcga
     9301 taacccggta cgtaagcctg caccgcctca gccatttcat tgattgaggc ttcgatatca
     9361 tcttgtgaag cttcgtcgct caatacatac accgtgtcac gcatcatcaa cggtggctct
     9421 gctgggttaa gcacaataat cgccttccct tttgccgcgc cgcccaccac ttcaatggct
     9481 cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg tgccaggtcc ggcagattta
     9541 ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg ccacgcgtga aactgccgcc
     9601 accattggaa tggtggcctg gccgccgcag gtgaccatgt tgacgttcag ttgatcgacg
     9661 ttcgcctcga ggttaaccac cggcacgcag taagggccga tggcagcagg cgtcaggtca
     9721 attaagcgaa tatccggttt cgcttcgcgt aaagcggcat cgtttttcac atgcgcgccc
     9781 gcgctggtcg catcaaatac aatgtcgata tcagcaaatt caggcatgtt catcagcccg
     9841 atcacccctt catgggtggt ggcgacgccc atacgtctgg cacgcgccag accgtcggac
     9901 tgaggatcaa tgccgaccat caccgccatc tccagatgct gaccgtgacg caaaatttta
     9961 atcatcagat cggtgccaat gttgccagaa ccgataatgg cgactttacg cttactcatg
    10021 acagacttcc ttttggggct gcgcttgaaa atgtcgcagc aactgaacct atgccttcaa
    10081 tatgggcttc aaaacgatcg cccgcattca ctgccaccat cggacctaat gccccggtaa
    10141 gaatgatatc tccggcgcgc agcggttcac ccaggctggc cattttgcgt gccagccaga
    10201 cggcggcatt aagcggatgt cccaggcatt cgctgccgcg cccgctggaa acctcttcgt
    10261 tattacgcgt catcttcatg gcgcagtttt tcaggtctaa ccccgccgga cgttgcgccg
    10321 gaccgccgat gacatacacc ccgcaggaag cgttatctgc cacggtatcg acaaactgaa
    10381 tcgaccagtc gcgaatgcgg ctacccacca cctccagcgc cggaagtacc cattcaatgg
    10441 cgttatacaa ttcgtcgaag gtgatatcgg ttgcgggcaa atcgcggttc aacaccagtg
    10501 cgatctccgc ttcaatgcgg ggttgcagaa cacgggaaaa aggaatgatt tcgttatcgc
    10561 cataacacat gtcggcaaat aatgtcccga aatccggttg atcaacgccc agttgttgct
    10621 gcactttcgg atgcgtcagg cccactttac gccctaccac gcgacgcccc tgcgcaacgt
    10681 catattgcac atttatgtgc tgaatggcgt aggccgcttc agcgttatcg ataccaatca
    10741 gatcgcgcag cggcgcaatc gcttcgccct gctctgcggc gcggcgtaaa tccgccgcca
    10801 gttgctcaag agtatgcttc gtcatgacca ttccttaagg gcgtgcgagg aaattcagca
    10861 ccaattgatt gaaagcgtcg gcatgttccc actgcgccca gtgaccacag tcgcggaaga
    10921 tatgcagttc agaaccggca atgccggaca gcagacgcag acccgcatcc atcggcacaa
    10981 agcggtcgtt gcgcccccag acaatcaggg tttgcgcttt gatttccgcc agacgtgggc
    11041 caaaatccgg gaactgtttc ggattagctt ccaggctctt aacgaagttt tccaggtgat
    11101 cgcggcgcga cagcatatta ttcaggcgcg cttcaaacag ggcgtcggtc aaatcgctgg
    11161 tatcgaaaac gaagatatcc atcatcagct tcaggttttc gatagtcggc tgacgataaa
    11221 gctgattcag tcgcttaata ccttcggttg gcatcggcgt aaacaaactc atgccgcccg
    11281 taccgccgcc catcagcacc agtttgccga cgcgctccgg ccagttaagg gtgaacgcca
    11341 cagaactatg gccgcccatc gagttgccca gcaggtggat tttggcgata tccagttgat
    11401 ccaccacgct tttcaggatt cgtgcattaa gatccgatcg tgaaccacta ttaacgatcg
    11461 aatcgctttt gccccaaccc ggacaatcca gcaggatcac ccgatagccc gcctctacca
    11521 gcggatcgat attgcggctg aagttcgccc agccagtagc acccgggccg gaaccatgca
    11581 gcaggacaac ggtttcgtcg ccttgtccgc agtcattaaa atggatgcgc agcgttttac
    11641 ccgcttcttc tacattcaga aaacggctgg tggcggcttc ggtttgtggc tgataactca
    11701 tcatcttctc ctgcatattc agttctctgt tctggcgctt aacgagccaa atccggcaat
    11761 ccactccggg attgggcggt aataacgccc ttcgctacgc cagttgccaa acgcagaaat
    11821 agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc gactttccgg caatagcgga
    11881 aagctcttcg ttactgacgg catccagttc ctgtatgcgt ccctgctcca gcaaagtcat
    11941 gaactggtta tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct caacaaactt
    12001 ctcagcggcg ctaatcaccc gttgctgacg caattcgcgc tcactggcgg gtaaatcttt
    12061 cccgctcccc aacagacggt cgcgcatatg ggcatcggct ttcgccagtt cgggcaccgg
    12121 cggctgatgg gaaagcccac cggaacccag gaacagcacg cgtttattga gagtgctggt
    12181 gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg aaaccgggca gcggcgtggc
    12241 gacaccgttg atgaacacag gcagaactgg caccttatcc agcccaccga gcaggaactc
    12301 cagcggctgg gcgaacccgt ggtccacctg catacagtaa gaaacggcaa gatcgatccc
    12361 gctcttcatg acagcatgcg cacaggcctc cgccagctcc acaggcacgg gcagctctcc
    12421 tgccgcactg ccgaaatcac caattgccgt cgctccaacg cctaaacaga acggtggcat
    12481 cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca gttcagggga
    12541 gaaagccgca atacgctcgc gggcgctggc aatcacgcca ttgacctcat cgagtacctc
    12601 ttgcgccggg tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa gataagcgtg
    12661 catatcaggc taccttttcg acagaaacgt cggcatcagg gcgggtcagc gtcatcaccg
    12721 acgccagttt attcagcgta ttgcccaggg tttgcggaat ggcggtggcg gcaacaaagc
    12781 gatccgggcg catcaccacc agcgaagcat tgtgttgcgc gaaccagcta cgcagacgac
    12841 cttgcgtatc gcccacgcgt agcacgccgt cgtggttatc ctgtgcggta tgaatttgca
    12901 cttccggcac cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg atttgctcgt
    12961 cgctcatccc ccacagtgga ttgcatcccc agccaattac cgcgaagttc gcgccaatcg
    13021 cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga ataaacatct
    13081 tgccgaccgg agagtgcttc gcttcgccct cacgtaccag cgcaccgccg taatattgcg
    13141 gcatcggctt gaagcgcatt tcgaggaagt agcgttttac tggcggcaga taattcagta
    13201 gccaggaaac gccgtcacgt aacgtaccct gccagcgttt cggcggagcc agcacgttgc
    13261 ccgccgtcac ggacaggtca atcatcgctt tggcgtgatc gcgacgttct tgttgatagg
    13321 tatcgagcag cgcatcgcgg gctttcccct ggataaccaa cgccagtttc caggcgaggt
    13381 taaaggcgtc gcgcatacca ctgttatagc cctgcccctg ccataccggc atgatgtgcg
    13441 cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt cgcgcgttgt
    13501 gggtgtagac acgctggcga atcaattcaa cattgtccgg attaggcagc actttgctta
    13561 acagcttgcg catattttgc ggctcacgca gctgttcttc ggtttctccc ggcatcacca
    13621 taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga aacatacggg cgcaccggat
    13681 cgcaacataa atagatatgc ggcgtactta acggatcgtt ggcgatatcc accacaatcc
    13741 actgatttgg cgcagtttta ccttcaaacg gcacattcag ggtgcgacga acaaaacttg
    13801 ccccaccatc acaggccacc agccactggg ctttgactat ttcccgctgc ccttctgccg
    13861 ttttcaggtg caaggtcact tcgtcatttt gctggctgaa ggcctccagc tcgcgggcaa
    13921 acaagcagcg cacattcgga aaacgcgaca acccttccag catcaccgca tcgacctgtg
    13981 gctgaataaa ggcgttacgg cgcggccagc caaattcatc ggtcattggc tgaatatcag
    14041 caaaacagcg gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta gtgtgcggca
    14101 gaacattctc gaccaggccg accgactgca tggtgcgcag cgcctcgtca tcaataccaa
    14161 tcgcacgcgg gtagtcgatc aacttatcga gtttctccac caccagcacg tcaatgccca
    14221 tctgaccgag atagttcgcc atcatcagcc caaccggacc ggcaccagcg atcgccacct
    14281 gaacgctatg gttaacagca ggctggatgt cagggtgttg tattgccatt tcagtacctc
    14341 acgactcgga caaaatgccg ttgcgcgcac agtacagcgc aacttatttt gttaaaaaac
    14401 atgtaaatga ttttttattg tgcgctcagt ataggaaggg tattttcggc tacaatcaaa
    14461 acatgcccga atgtgcacca ggtgcacaac gttgttttaa ctatagaaat gtcaattaat
    14521 atgcagaaca atgagcagac ggaatacaaa accgtgcgcg gcttaacccg cggtctaatg
    14581 ttattaaata tgttaaataa acttgatggc ggtgccagcg tcgggctgct ggcggaactc
    14641 agcggcctgc atcgcaccac tgtgcggcga ctgctggaga cgctgcagga agagggatat
    14701 gtccgccgta gcccctccga tgatagtttt cgactgacca tcaaagtgcg gcaattaagc
    14761 gaaggatttc gtgacgaaca gtggatttct gcactggcgg ccccgctgct gggcgatctg
    14821 ttgcgcgaag tggtatggcc aacagatgtg tccaccctgg atgttgatgc aatggtggta
    14881 cgcgaaacca cccaccgttt cagccgctta tcctttcacc gggcaatggt cgggcgacgt
    14941 ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg ccttttgccc ggaacaagaa
    15001 cgcaaggaat taatcgaaat gttagccgcc cgccccggtg atgactatca actggcacga
    15061 gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca aagagggtta cggacagaac
    15121 taccgcggct gggatcagga ggagaagatt gcctctatcg ccgtaccgct gcgcagtgaa
    15181 caacgggtga ttggctgtct gaatctggtg tatatggcga gcgcaatgac cattgaacag
    15241 gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa aacagatcga agaaggggtt
    15301 gaatcgcagg ctattctggt ggccggacgg cgaagcggcg tgcatttacg ttgacaccat
    15361 cgaatggcgc aaaacctttc gtggtatggc atgatagcgc ccggaagaga gtcaattcag
    15421 ggtggtgaat gtgaaaccag taacgctata cgatgtcgca gagtatgccg gtgtctctta
    15481 tcagaccgtt tctcgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgagaaaa
    15541 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
    15601 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
    15661 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
    15721 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
    15781 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
    15841 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
    15901 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
    15961 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
    16021 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
    16081 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
    16141 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
    16201 taccgagtcc gggctgcgcg ttggtgcgga tatctcagta gtgggatacg acgataccga
    16261 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
    16321 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
    16381 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
    16441 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    16501 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    16561 ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat tgtgagcgga taacaatttc
    16621 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
    16681 gactgggaaa accctggcgt tacacaactt aatcgccttg cggcacatcc ccctttcgcc
    16741 agctggcgta atagcgaaga agcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    16801 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
    16861 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
    16921 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
    16981 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
    17041 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
    17101 aacggtcgct gggtcggtta cggtcaggac agtcgtttgc cgtctgaatt tgacctgagc
    17161 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc
    17221 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg
    17281 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcactt taatgatgat
    17341 ttcagccgcg cggtactgga ggcagaagtt cagatgtacg gcgagctgcg cgatgagctg
    17401 cgggtgacgg tttctttgtg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
    17461 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacgct acgtctgaac
    17521 gtcgaaaacc cggcgctgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
    17581 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
    17641 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt
    17701 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
    17761 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac
    17821 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat
    17881 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg
    17941 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
    18001 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg
    18061 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
    18121 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
    18181 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagaactgcg cccactgatc
    18241 ctttgcgaat atgcccacgc aatgggtaac agtcttggcg gtttcgctaa atactggcag
    18301 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
    18361 ctgattaaat atgatgaaaa cggcaacccg tggttggctt acggcggtga ttttggcgat
    18421 acgccgaacg atcgccagtt ctgcatgaac ggtctggtct ttgccgaccg cacgccgcat
    18481 ccggcgctga cggaagcaaa ataccagcag cagtttttcc agttccgttt atccgggcaa
    18541 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg
    18601 atggtggcgc tggatggcaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca
    18661 caaggtaaac agttgattga actgcctgga ctaccgcagc cggagagcgc cggacaactc
    18721 tggcttacgg tacacgtagt gcaaccgaac gcgaccgcat ggtcagaagc cggacacatc
    18781 agcgcctggc agcagtggcg tctggcggaa aacctcagcg tgacactccc cgccgcgtcc
    18841 cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag
    18901 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa
    18961 caactgctga cgccgctgcg cgatcagttc acccgcgcgc cgctggataa cgacattggc
    19021 gtaagtgaag cgacccgcat tgacccgaac gcctgggtcg aacgctggaa ggtggcgggc
    19081 cattaccagg ccgaagcggc gttgttgcag tgctcggcag atacacttgc cgacgcggtg
    19141 ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa
    19201 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtagcgagc
    19261 gatacaccgc atccggctcg gattggcctg acctgccagc tggcacaggt agcagagcgg
    19321 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt
    19381 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
    19441 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
    19501 ttccagttca atatcagtcg ctacagccaa caacaactga tggaaaccag ccatcgccat
    19561 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
    19621 ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac
    19681 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
    19741 tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
    19801 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
    19861 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
    19921 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cgggctgcgc
    19981 aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt ctttattttt
    20041 atcttcgggc cactgttaca atacaacatt ttagtaggat cgattgttgg tggtatttat
    20101 ctaggctttt gttttaacgc cggtgcgcca gcagtagagg catttattga gaaagtcagc
    20161 cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg ctgggcgctg
    20221 tgtgcctcga ttgtcggcat catgttcacc atcaataatc agtttgtttt ctggctgggt
    20281 tctggctgtg cactcatcct cgccgtttta ctctttttcg ccaaaacgga tgcgccctct
    20341 tccgccacgg ttgccaatgc ggtaggtgcc aaccattcgg catttagcct taagctggcg
    20401 ctggaactgt tcagacagcc aaaactgtgg tttttgtcac tgtatgttat tggcgtttcc
    20461 tgcacctacg atgtttttga ccaacagttt gctaatttct ttacttcgtt ctttgctacc
    20521 ggtgaacagg gtacgcgggt atttggctac gtaacgacaa tgggcgaatt acttaacgcc
    20581 tcgattatgt tctttgcgcc actgatcatt aatcgcatcg gtgggaaaaa cgccctgctg
    20641 ctggctggca ctattatgtc tgtacgtatt attggctcat cgttcgccac ctcagcgctg
    20701 gaagtggtta ttctgaaaac gctgcatatg tttgaagtac cgttcctgct ggtgggctgc
    20761 tttaaatata ttaccagcca gtttgaagtg cgtttttcag cgacgattta tctggtctgt
    20821 ttctgcttct ttaagcaact ggcgatgatt tttatgtctg tactggcggg taatatgtat
    20881 gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc tggtggcgct gggcttcacc
    20941 ttaatttccg tgttcacgct tagcggcccc ggcccgcttt ctctactgcg tcgtcaggtg
    21001 aatgaagtcg cttaagcaat caatgtcgga tgcggcgcga gcgccttatc cgaccaacat
    21061 atcaggacgg aatgatcgca atgaacatgt cgatgacaga aagaataaaa gcaggcaagc
    21121 tatttaccga tatgtgcgaa ggcttaccgg aaaaaagact tcgtgggaaa acgttaatgt
    21181 atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag agaaagcctg attaaagaaa
    21241 tgtttgccac ggtaggggaa aacgcctgga tagaaccacc tgtctatttc tcttacggtt
    21301 ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt gtcgacgact
    21361 acacggtaac gatcggtgat aacgtattga ttgcacccaa tgttactctt tccgttacgg
    21421 gacaccctgt acaccatgaa ttgagaaaaa acggtgagat gtactctttt ccgataacga
    21481 ttggcaataa cgtctggatc ggaagtcatg tggttattaa tccaggcgtc accatcgggg
    21541 ataattctgt tattggcgcg ggtagtgtcg tcataaaaga cattccacca aacgtcgtgg
    21601 cggctggcgt tccttgtcgg gttattcgcg aaataaacga ccgggataag caatattatt
    21661 tcaaagatta taaagttgaa tcgtcagttt aaattataaa aattgcctga tacgctgcgc
    21721 ttatcaggcc tacaagttca gcgatctaca ttagccgtat ccggcatgaa caaagcgcag
    21781 gaacaagcgt cgcatcatgc ctctttgacc cacagctgcg gaaaacgtgc tggtgcaaaa
    21841 cgcagggtta taatcatcag cccaacgacg cacagcgcat gaaatgccca gtccatcagg
    21901 taattgccgc tgatactacg cagcacgcca gaaaaccacg gggcaagccc ggcgatgata
    21961 aaaccgattc cctgcataaa cgccaccagc ttgccagcaa tagccggttg cacagagtga
    22021 tcgagcgcca gcagcaaaca gagcggaaac gcgccgccca gacctaaccc acacaccatc
    22081 gcccacaaga ccggcaattg cagcggcaac cagataaagc cgcagaaccc caccagttgt
    22141 aacaccagcg ccagcattaa cagtttgcgc cgatcctgat ggcgagccat agcaggcatc
    22201 agcaaagctc ctgcggcttg cccaagcgtc atcaatgcca gtaaggaacc gctgtactgc
    22261 gcgctggcac caatctcaat atagaaagcg ggtaaccagg caatcaggct ggcgtaaccg
    22321 ccgttaatca gaccgaagta aacacccagc gtccacgcgc ggggagtgaa taccacgcga
    22381 accggagtgg ttgttgtctt gtgggaagag gcgacctcgc gggcgctttg ccaccaccag
    22441 gcaaagagcg caacaacggc aggcagcgcc caccaggcga gtgtttgata ccaggattcg
    22501 ctatgttgaa ctaaccaggg cgttatggcg gcaccaagtc caccgccgcc catcagagcc
    22561 gcagaccaca gccccatcac ctgtggcgtg cgttgctgaa accgccgttt aatcactgaa
    22621 ggcatcaccg cctgaatgat accgatcccc accccaccaa gcagtgcgct gctaagcagc
    22681 agcacacttt gggggtaaag ctcacgcatc aatgcaccga cggcaatcag caacagactg
    22741 atggcgacgc tgcgacgttc gctgacatgc tgatgaagcc agcttccggc cagcgccagc
    22801 ccgcccatgg tgaccaccgg cagagcggtc aacagggcag ccacgctaaa gctcattccg
    22861 ctcgcctggc gcaattgcgg tagcagtgga ccgacggagg tgagcagtgg tcgcatatta
    22921 agaccaatca gcaccagaac cagcagcatc gtgaacgtct tttgatgatt gcgcatggct
    22981 gtcagaacgg cttggtcggc agatatttac catctaaggt aatgaccgca cgttcgccac
    23041 cttccgggtc cgccactttc ttaacgtcga gtttgaagtt aatcgcgctg ataatgccat
    23101 cgccaaactt ctcatgaacc aacgctttca gggttgtgcc gtacacctgc aacatttcat
    23161 agaaacgata catcgttggg tcggttggaa tacggtcatc aatgcagcca cgcagtggaa
    23221 tcatctgcaa cagtagaatg gcgtcttcgt cgagatccag cttcgccccg accaggcggg
    23281 cggcgtcggc aggaagcgct tgctgcccca gcaaagccgc ggttacgaag gcttctgcca
    23341 gaccggtacc gtcggcaatc tcggcaaatg agagatcttt tttagctttg ctgagcaaaa
    23401 tggcatcggc aagatcaaga cgaatattgc ggttaatttg tgactgaatc atggtggatc
    23461 tcctgatggt ttaaaaataa ggttacgctg cggtcggttg gcgtagcggt atggcacaaa
    23521 cgcgaggatt agcggccagt ggcacaaact ggcgggttgc gccgtcaaaa gctgcgatgc
    23581 tgccgctttc aatgtcgtag acccagccgt gcagggcgat ccgcccctct tcgagcgcca
    23641 ggcgcaccga tggatgagtt tgcaaattag ccaactgagc aatgacgttt tcacgtacca
    23701 tcgccgcagc ttttgacggt aaatcggaat gcgggcgcgc ctcattaacg acgcgggctg
    23761 aatcggcata acgcagccag tgggagacgg caggcatatg gtccatgcac tgacagctgg
    23821 caatggcggt catcgcgcca cagttggaat gaccacaaat cacaatgtca gatacccgaa
    23881 gcgcagcgac ggcatactcc accgaagcag aaacgccacc gggttccggc ccgtaggaag
    23941 ggacgatatt gcccgcgttg cgaataacga acagatcgcc aggctcacgt tgcgtcacca
    24001 gctcagggac cagacggctg tcggagcagg agataaaaag tgtgcgcggg ctttgctgtg
    24061 tcgccagctg tttaaacaag gcttcccgct tcggaaatgc ctcgcgctgg aatttaagga
    24121 atccatcaat aatctctttc accgttaacc tctgtctgtc tctggaatga gaggcagact
    24181 actcgccgat tgtcataagg taaaagtctc atttatgatg agttctattg gatttactta
    24241 taggttggcg aatgctctct cgacatatca actattttct tgccgtggct gaacatggca
    24301 gctttacccg tgccgccagt gcgttgcacg tctcccagcc tgcgctttcc cagcagattc
    24361 gccagttaga ggagagttta ggcgtgccgc tgtttgaccg tagcgggcga acgattcgtc
    24421 tcactgatgc aggagaagtc tggcgacagt acgccagccg ggcgttacag gaactggggg
    24481 cgggtaaacg ggcgattcat gatgttgccg atctgacgcg aggatcgctg cgtatcgccg
    24541 tcactcccac ctttacaagc tactttatcg gccctttaat ggcggatttc tatgcgcgct
    24601 accccggcat cacgctccag ctacaggaaa tgtcgcagga gaaaatcgaa gatctgcttt
    24661 gtcgcgacga gctggacgtt gggattgcct tcgcgcctgt gcattcgccg gagctggagg
    24721 caattccttt actgacagaa agtttagcgt tggtggtggc gaaacatcat ccgctagctg
    24781 cctgtgaaca ggtggcgttg agtcgcttgc atgatgaaaa actggtcctg ctcagcgcgg
    24841 aatttgccac cagagagcaa attgaccact actgcgagaa agcggggcta catccacagg
    24901 tggtcattga ggcgaactca attagcgcgg ttctggagct gattcgccgc acttcccttt
    24961 ccacattgtt accagcagcg attgccacac aacatgacgg gcttaaagcg atttctcttg
    25021 ccccgccact actggagaga acggcggttt tgttgcggcg gaaaaatagc tggcagacag
    25081 ccgccgcgaa ggcatttttg cacatggcgt tggaggaatg tgcggatgtt ggcgagaatg
    25141 aatcacggta gccactgaca caggtcagtg gctcttcaac acagaggaaa ttaccgtttg
    25201 taatcgatgg cttctggctg ctccagatat acggtggttt gtgccggttg tgtactggca
    25261 atcaccttgc cgccacgtac cgaataacgt accggaacct gacggcgcag cgcatcaaat
    25321 ccattttcag ccggcaggat aatcaggttt gcgctgtttc cggcggcaat gccgtaatcc
    25381 tgcaaattca atgttctggc actgtggtgg gtgattaaat tcaggccatc attaatctgc
    25441 ccgtagccca tcaactggca aacatgcagc cccatatgca gcacttgcag catattcgcc
    25501 gttcccagcg gataccacgg atcgaagaca tcatcgtgac caaagcagac gttaatgccg
    25561 gactccagca tctctttaac gcgcgtgatg ccgcgacgtt ttggatacgt atcgaaacgt
    25621 ccttgcagat gaatattgac cagcgggttg gcgacaaagt taataccgga cattttcagc
    25681 aagcggaaca ggcgtgaggt atacgccccg ttataggagt gcattgccgt ggtgtggctg
    25741 gcggtgactc gcgcgcccat gccttcacgg tgcgccaggg cagcaacggt ttcgacaaag
    25801 cgcgactgct cgtcatcgat ctcatcacag tgaacgtcga tgagacggtc gtatttttgc
    25861 gccagggcga aggttttatg cagcgactcc acgccgtatt cacgggtaaa ttcaaaatgc
    25921 ggaatcgccc ccactacatc tgcccctaag cgtaacgcct cttccagcaa cgcttcaccg
    25981 ttgggatacg acaaaatccc ttcctgaggg aaggcgacaa tttgcagatc aatccacggc
    26041 gcgacttcct gcttcacttc cagcattgct ttcagcgcag ttagcgttgc atccgaaaca
    26101 tcgacatggg tacgcacatg ctgaatgccg ttggcaatct gccatttcag cgtttgccag
    26161 gcgcgttgtt tcacatcgtc atgggttaat aacgctttgc gctcggccca gcgttcaatg
    26221 ccttcaaaca gcgtgccgga ctgattccag ttcggttgtc cggcggtttg cgtggtgtcc
    26281 aggtgaatat gcggctccac aaacggcggt ataactaaac cttgttcggc atccaggctg
    26341 ttttcagtta tgggcatcac gccggattgc gcatcaatgg cgctgatttt tccgtcctgc
    26401 agatgaatct gccacagccc ctctttgcct ggtaaccggg cgttaataat tgtttgtaaa
    26461 gcgttattcg acactgttaa cctccacatg cgtcattgct gctgtcgttt tacgattcaa
    26521 aatcgggtta aggatcagat agctcagcgc gccacctaat accgcgttga ccggaacaat
    26581 tcccggtaac cagtggcctg cggcaatccc cagcgcgacc gccagaatcg ccacccaatt
    26641 gacactcatc atacgcgtgg tcgcaaagtg ctcatagcgg cgacggttca tcagatagtc
    26701 ggcgatgatc acgcccccta ccggaggaat agctgccgaa aggaaggtca accagccgac
    26761 aaaattgtta tacagccata atgcgcagac cgtaccgata ataccgttga ttaccgaaag
    26821 ggttttgctc gacataccgg taatgttggc gaaacctaaa cccgacgcat agagtgcgtt
    26881 atcgttggtg gtccagatat tcagccccag caccacaatc gcaggcagca gcaggccctg
    26941 agcaatcatc acatccgaga tatccgccat gcccagtgcc gcagcgcccg ctgcaccgaa
    27001 aataaacatc aacgagttgc cgaggaaaaa ggccaccatc gccaccagca ccgccagttt
    27061 ggcattgcga ccaaaccgga caaagtcagc ggtgagcgta cccgcactga taaatgaccc
    27121 cacaaccagc gccagcgcga cattgaaatc taacggttgt gcgggaacga ccgcttttaa
    27181 tgcgtccagg ccgcccatgc cgttaacagc cagccacacg gaataaccgc ccaggcaggc
    27241 gatagccgga accgcaatca ccgaaagaac cgtcagcgcc gaaatgccaa aaaagacggt
    27301 gacggtcatc agtaaaccgg aaacggcaat cagcaaatta atatccagcc cggttgcctt
    27361 acccaccgga atggcaaaca tcgccacacc gacgccaaac cagccaacct gagttccgcc
    27421 cagtagcagt gaaggcagcc atgagccttt aacaccaaac gagaagcgag caagaagatg
    27481 agtggtcagg ccggtttttg cgccaatgta accgagaaat gaagtgtaaa taccgaggag
    27541 aagattaccg atgagaactg cgaggaagaa atcatgatag ctaagaccgg ttccgagagt
    27601 gccgccggtc cacatactgg cggaaaagaa ggttaatccc agcatgacga acgtcaatgc
    27661 caatacccct ttccgcgccg actgcgggac tggcccctgg ctaaagttgt tatcttgcga
    27721 cacgaaattc cccctttttg ctgtttgaaa cccaaaaaat ccgccgcatt ctattcatct
    27781 gaagataaaa agcaatcgtt ttcgtgggga aatatatttt ttatatgaat gagtgcaaaa
    27841 aggtggggga aaaattatcg gtgtaaaaac agtgggtagg cataataaga cgcggtaagc
    27901 gtcgcatcag gcatcggcgc atggtgtcgg atgcgccacg cgacgcatcc gacaatacct
    27961 tactcttcca tcgcctggcg gatctgatcc aacgacgcag gatcatcaat ggtcgtcaga
    28021 tccccaggat cgcgtccttc gcaaatcgcc tggatcgtgc ggcgcagcat ttttccggat
    28081 cgcgtttttg gcaattgcga gacaaaccag acgtgcgccg ggcggccaaa gttgccaatc
    28141 tggctgtcca ccagcgccat aatcgccttc tcttgcgagt gcgccacatc acgatcttcc
    28201 agactgtcgc tctctttcgg aatgacaaac gccaccgcca cctgcccttt cagcgcatct
    28261 ttcaccccaa ccaccgccac ttcggcaacg cccggatggc tggagatact ctcttcaatc
    28321 tcgcgcgtcc ccagccgatg cccggcaacg ttaattacat cgtcagtgcg cccgagaata
    28381 aagtgataac cgtcagcgtc acggatgccc cagtcaaagg tggcgtacac cgggcgggaa
    28441 aacagcgacc agtaagtctt cacaaagcgg ccgtcgtcgc cccagatggt ctgaatacac
    28501 cccggcggca gcggcccttc caccaccagc atccctttct cgttgacgcc acacggttcg
    28561 ccggtgactt cattaagcaa ctgcacgtta tagccataca tcggcacacc ggggcttccc
    28621 agacgcgtcg gcctgtcgtc cagaccgcga gcaatcgcca taatcggcca gccggattcg
    28681 gtctgccagt agttgtcgat gaccggcaca tccagcgtat tgctcaccca actggcggtc
    28741 ggctcgtcca gcggttctcc agccagatag agcacttcca gcgacgagag atcgtgtttg
    28801 cgaatttcag cggtagggaa ttttttcagc acgcgaatgg cggtcggcgc tgagaacatc
    28861 cggctaacct gatatttctc gacgattttc caccacacgc cgcagtccgg ccaggtcggt
    28921 aatccttcgt aaacgatagt cgccatcccc gccagcagcg gcgcgtagac gatatacgaa
    28981 tgccccacca cccagccgat atccgaagca caaaagaaca caccgcccgc tttgccgcca
    29041 aaaatggtgt ccatcgaggt cgccagcgcc accgcatatc cgccgacgtc acgctgcacg
    29101 cctttaggtt tgccggtcgt gccggaggtg tagagaatgc aggaggtttc gttggattcc
    29161 agccacgcca ccggtacccg cgcgccgatg tgttgatggc gcaacgacgc gaaatcgaca
    29221 tcccgcccgc tgacgcgtgc cattttcgcc agcccgcgat ccaccagcaa aacatggcgt
    29281 ggctggtgct gcgcctgact tatcgcatcg tcgagcaatt ttttataggg aatgattttg
    29341 ccaccgcgcg ctccggcatc agccgagaca atcagcaccg gtttagcatc atcaattcgc
    29401 gccgccacgc tgtgcgaggc aaatccccca aacaccaccg aatgaatagc gccaatgcgc
    29461 gcgcaggcca gcagagtaat atgcgcttcg gcaatcatcg gcatatacac cagcacccga
    29521 tcgccacgct gcacgcccag cgagcgcagc attgacgcca ccgcgtttac ttcgtcatga
    29581 agctgacgaa aggtaaaggt gcgctcttct tctgtttccg aagagacggc aatcagcgcc
    29641 agcgcctctg gttgtttctc cagccagcgg tcgatagcgt tgtggcacaa gttggttcgg
    29701 ccttcacaaa accaacgggc aaacggcgga ttgctgtgat cgagcgtttg cgtaaagggc
    29761 gtctgccagt caatacgccg ggcctgctcg gcccagaact gctccggttc gttaatcgaa
    29821 cgctgataaa attcgctaaa agacataatg ctctcctgtt gaacttacgc cttaccgctg
    29881 tttgcttaaa tgacgtacag gtcgagatac tcattgaccg gcatctgttc caggcgagtt
    29941 ctgtcgagag aaacctccag aatgcgctgc tgttggcgag tcgggaactg gcgcgcgaga
    30001 ttgattttga atttatcgac cagtttcgga ataccatcct gacggcggcg agcatgacca
    30061 atggggtact ccaccaccac ttcttcaaat cgtgtgccgt cggtgaactc aagggtaatg
    30121 gcattggcga tggcgcgttt ttccgggtcg tggtagtcag cggtaaatgc cggatcttca
    30181 aagcaattga tcttctcgcg cagggcgtcg atacgtttat cttgcgccac gttgtcctcg
    30241 taatctgccg ccgttaagcg cccgaacagc agcgggatcg ccaccatgta ctgaatgcag
    30301 tgatcgcggt ctgccgggtt attgagcggt ccttttttgt cgatgatgcg aatacaggct
    30361 tcgtgggtgc gaatggtcac tttttcgata tccgccgccg ttttgcctgc tgcctgcatc
    30421 tgttcataga gcgtcatcgc tgcttcaact gccgtctggg agtggaactc cgccgggaag
    30481 gagattttga acagcacatt ttccataacg taggaaccgt acgggcgctg gaagcggaac
    30541 gattcacctt taaaggagac gtcgtagaag ccccacaccg gcgcagtcag ggctgacggg
    30601 taacccattt cgcccgtttt cgccatcagt gccagacgta ccgcgcggga agtggcatcg
    30661 cccgccgccc aggatttacg cgtgccggtg ttcggcgcat ggcgataggt gcgcagcgac
    30721 tgaccgtcca cccacgccag cgaaacggcg ttgagaattt cctcgcgggt caggccgagc
    30781 atttcggcga ccacggcggt ggaagccact ttcactaaca gaacgtggtc gaggccgacg
    30841 cggttaaagg agttttccag cgcgatgcag ccctgaattt catgggcttt gatcattgcg
    30901 gtcagcacct gtttcatggt caacggcgct ttgccgctgg cgaccgcgtt gcgcgaaagc
    30961 cagtccgccg ttgccagaat gccgccgagg ttgtcggaag gatggcccca ctccgccgcc
    31021 agccaggtat cgttgaaatc gagccagcgg atcatcgcgc cgatgttaaa tgccgcctgg
    31081 acggggtcga gctggaactg agttccgggg acgcgcacgc cgttgggtac gacagtgccg
    31141 gggacaattg gccccagcag ttttttacag gccgggtatt cgagagcttc aagaccgcag
    31201 ccgagcgtgt cgagcaggca gtaatgggcg gtgtcgtagg ctactttgga gctgatttcg
    31261 tagttcatca cgtaatcgac gatatcaacg atttcacgat caaattccgg gcggatgttg
    31321 ttgatttgag ctgacattgg gtacgtttcc ttattgttat tcgtagaggt ttactggcgc
    31381 ttatccagcg cgacaaactg gcggtcttcc ggtccaacat aattggcgga aggacggata
    31441 attttgttgt cctgacgttg ttcgataatg tgcgccgccc agccggtgac gcgggcgata
    31501 acaaacagtg gtgtgaacat ctcggtggga acgcccatca tgttgtagga aacagcggag
    31561 aaccagtcga gattggggaa catctttttg ctctcccaca tcactgtttc caggcgatcg
    31621 gcgatgttat acatcttcag cgaaccgcct tcctgcgaga gctgcttcgc cacacgttta
    31681 atcacctggt ggcgcgggtc agcgatggtg taaaccggat gaccaaaacc aatgaccact
    31741 tctttgtttt ccacgcgctt gcggatatct gcttcggctt cgtccggcgt ttcgtagcgt
    31801 tgctggatct ccagcgacac ttcattcgcc ccgccgtgtt ttggcccgcg cagtgcgcca
    31861 atcgcgccaa taatcgcgga atacatatca gagccggtgc ccgcaatcac ccgactggta
    31921 aaggtggagg cgttaaactc gtgttcggcg tacagcacca gcgagatatg catcgccttt
    31981 tcccagcttt gcgatggctt ttcgccgtgc agcagatgca ggaagtgacc gccgatggag
    32041 tcgtcatcgg tttccggctg gatgcgttcg ccgttgtgac tgtagtgata ccaataaagg
    32101 agaatcgagc taagtgatgc cagcagtttg tcggcaatat cccgcgcgcc agagacagtg
    32161 tgcccctctt tttctggcag tgtgcagccg agcgcggaaa cgccggtgcg catgacatcc
    32221 atcgggtgcg acgccgccgg taaggcttcc agcacggtac gcacattagc tggtaaacca
    32281 cgcagggctt tcagtttcgt tttgtaagcg gcgagttcgt cacgggtcgg cagtttgccg
    32341 tggatcagca ggtgcgccac ttcttcaaat tcgcaatgtt ccgccagatc aagaatatcg
    32401 tagccacggt aatgcaggtc attgccactt ttacccacgg tgcagagcgc cgtattgccc
    32461 gccggaacgc cagaaagtgc taccgatttt ttcggtttaa tgacatgggt actgttttgc
    32521 aggatcgttg tgtcgctcat attgtcctcg tcatttgtag ggtcgagata tttttcgttg
    32581 tgttttattt cacctggcta cgggcaaaca ggtcgtcgag cttctcttcg tactggtagt
    32641 agttgatgct ttcgtacagc tcgttgcggg tttgcatggt atcgatgacg cttttctgcg
    32701 tgccttcctg acgcaggacg ttgtagacat gttcagcggc gcggttcatg gcgcggaacg
    32761 ctgaaagcgg gtacagcgcc attgcgacat gggcgctgcg taattcgtcg gtggtaaaca
    32821 gcggtgttgc gccaaattcg gtaatgttgg cgaggattgg cacctgcacc gcatcggcaa
    32881 actggcggta catggcgagt tcggtaatcg cctccgggaa caacatctcg gcacccgctt
    32941 caacataggc ctgcgcacgc tcgatcgccg catccagccc ctctaccgcc agagcatcgg
    33001 tgcgcgccat gatcacaaaa tcaggatcgg ttttcgcatc caccgccgcg cggatccgat
    33061 ccaccatctc ttctttcgag acgatcgctt tattcggacg atgaccgcag cgtttcgcac
    33121 caacctgatc ttcaatatgc aatcctgccg caccggcttt aatcatcgat ttcacggtgc
    33181 gcgccacgtt aaaggccgaa gaaccaaaac cgatatccgc atccaccagc agcggcagcg
    33241 aacaaacgtc ggtgatacgg cgaatgtcgg tcagcacatc atcaagggta gaaataccga
    33301 gatcgggcag ccccagcgaa cctgccgcca cgccaccgcc agaaagataa attgcctgat
    33361 atccggcacg ctgcgccaac agcgcatgat tagcgttgat ggtgccaaca atctgcaatg
    33421 gattttcttt agtcagtgca gcgcgaaacg ctttacctgg agagtgtaga gacatagccc
    33481 atcctttgtt atcaacttgt tatttgcgtt gataaagaca aagcaagggg tgtgccacaa
    33541 ttgccttaat aaaattaaat gtttaatttc aatttgttag agataaagtc atgaactaat
    33601 ccgcaaatat gcgtttcagt taacgtttca ggcaatgttt caccgcgttt cattgcaaca
    33661 attatgaaac aagactaaac ccaatattcg gtttcttaac tttgcggtgc gctatggcac
    33721 atccaccacg gcttaatgac gacaaaccgg ttatctggac ggtatctgta acgcgcctgt
    33781 tcgagctgtt tcgcgatatc agcctcgagt ttgatcacct ggcgaacatt acccctattc
    33841 agcttggctt tgaaaaagca gtgacctaca tccgcaagaa actggcaaac gaacgctgtg
    33901 acgccatcat cgcggctggc tctaacggcg cgtacctgaa aagccgcctg tcagtgccag
    33961 ttattttgat taaaccgagc ggctacgatg tgttacaggc actggcaaaa gccggaaaac
    34021 tcacctcttc tatcggcgtt gtcacctatc aggaaaccat tccagcgctg gtggcgtttc
    34081 aaaaaacctt taatttgcgc ctcgatcaac gtagctacat taccgaagaa gacgcacgcg
    34141 ggcagattaa cgagctaaaa gctaacggca ccgaagcggt ggtcggcgcg gggctgatta
    34201 ccgatctggc agaagaagcc ggaatgaccg gaatttttat ctattccgcc gctaccgtgc
    34261 gccaggcgtt cagcgatgcg ctggatatga cgcgcatgtc gttacgccat aacactcacg
    34321 atgccacccg caacgccctg agaactcgtt acgtgctggg cgatatgctc ggtcaatcac
    34381 cacagatgga acaagtacgg cagactattt tgctgtatgc ccgctccagt gcagcggtgt
    34441 tgattgaggg ggaaacgggg acgggcaaag agctggcggc ccaggcgatt catcgggaat
    34501 attttgcccg ccacgatgcg cgacagggca aaaagtcgca tccgtttgtt gcagtcaact
    34561 gcggggcgat tgccgaatcg ctgctggaag cagaactgtt tggctatgag gaaggggcgt
    34621 ttaccggctc gcgacgcggc ggtcgcgccg ggctgtttga aatcgcccac ggaggtacgc
    34681 tgtttctcga tgagattggc gaaatgccgc tgccgttgca gacccggctg ctgcgggtgc
    34741 tggaagaaaa agaggtcacc cgcgtcggcg ggcatcagcc tgttccggtg gatgtgcggg
    34801 tcattagcgc cactcactgc aatctggaag aagatatgcg gcaagggcag tttcgccgtg
    34861 acctgtttta tcggctgagt attttgcgtc tgcaattgcc accactgcgc gagcgggtgg
    34921 cggatattct gccactggcg gaaagctttt tgaaagtgtc tctggcggcg ctctccgccc
    34981 cgttttctgc cgcattacgc cagggattac aggcaagcga aaccgtgctg gtgcactacg
    35041 actggccggg caatattcgt gaactgcgca atatgatgga gcgactggcg ctatttttaa
    35101 gtgtggaacc gacgccggat ttaacgccgc aatttttgca gctgctactg ccggaactgg
    35161 cgcgcgagtc ggcgaaaact cccgctccac gcttactgac accacaacag gcactggaga
    35221 aatttaatgg cgataaaaca gcagcggcga attatttagg catcagccgg acgacgttct
    35281 ggcggcggct gaaaagctga actgccgaat gcagcgcgaa cgctttatcc ggcataaatt
    35341 tcaatagatg atgatcaccg ataaatttac gcatcgggga tgtttacata gggttcagaa
    35401 ttacttcttc ttataaatat ttgccgtgcc gtggatctta ttgtcagttt gaccagaggt
    35461 cagtaccaac acatcagccc ctttttcatc cgcttttttg atcaaatcct ctttcgcatc
    35521 tgcggtcgac atttcattac tggttgaaat atcacctatt ttttcatact gcgattcaac
    35581 tttttcaaat tccgctttgg tcatcaattc agcggcatag acattggtaa cggcaaacgc
    35641 taacgcaccg actaacattt tagagattat tttcatatta cggctccaga gttgttatca
    35701 cactatcacc cacccacatt attgggttga ttaataatta gttcagtcag ggcataaaag
    35761 ccagtaaccg gaaataagac actttgtgat ctctttcgca cccgattatt tttggtagaa
    35821 atatgatgaa gttacttcac ttatttatgg atgaaatcac tatggatcct ttgcacgccg
    35881 tttacctgac cgtaggactg ttcgtgatta ctttttttaa tccgggagcc aatctctttg
    35941 tggtagtaca aaccagcctg gcttccggtc gacgcgcagg tgtgctgacc gggctggggg
    36001 tggcgctggg cgatgcattt tattccgggt tgggtttgtt tggtcttgca acactaatta
    36061 cgcagtgtga ggagattttt tcgcttatca gaatcgtcgg cggcgcttat ctcttatggt
    36121 ttgcgtggtg cagcatgcgc cgccagtcaa caccgcaaat gagcacacta caacaaccga
    36181 ttagcgcccc ctggtatgtc ttttttcgcc gcggattaat taccgatctc tctaacccgc
    36241 aaaccgtttt attttttatt agtattttct cagtaacatt aaatgccgaa acaccaacat
    36301 gggcacgttt aatggcctgg gcggggattg tgctcgcatc aattatctgg cgagtttttc
    36361 ttagtcaggc gttttctttg cccgctgtgc gtcgtgctta tgggcgtatg caacgcgttg
    36421 ccagtcgggt tattggtgca attattggtg tattcgcgct acgcctgatt tacgaagggg
    36481 tgacgcagcg gtgagcagat aatttactct ctacgtaatc aacctgattt gccgaatccc
    36541 ggccatcggc gatgatagcc cccgtaatgc gtggtcctta tttccgataa gtttacttga
    36601 gtctttagct tcccatttca gcccactctc ccctgtgtgg gcttttttca ttggtctgcc
    36661 ccgtcctgaa tagccaaaca atggcaatgt tccactttgc agggatgata ataaggggat
    36721 ctgattcaga agagttttga aaagttggac cgccatcgag gcctcgaacc ccgtaccata
    36781 caacaatcct ggttgtcgtc tgctcttcct gatgagctaa tggcggtttt tgatatgagc
    36841 cttacagcat attttctgtc aggcttgagt gcgatgcgta aggtacagaa aagaccccac
    36901 acaaacatga tggaattatg atttttttca tatgaatgtc aacccacaca ttcacaaaac
    36961 atctgcatga gatcattttt tatacaccgc atagctgcga aaccactgct ggtgcttttt
    37021 taagcgaagc gtcgccgatc acaacaaaat atcggcgaaa aaggcggtgg aatgccattt
    37081 ggcatacgga tatacagatg aaatgattac ctgctttgcg attcactctc tggtggaata
    37141 acaacttgca ggtggaaccc tttctgaact aaagagtgta gcggatttta acgtgcttat
    37201 ttacaggata atatctgtca cctaattcct gcactctatt cgatatccat tcatttaatc
    37261 gtgcgtcatc tctgtatgtt tttacgtcca gaacagagag caataacatc accagtgatt
    37321 caggaaaaac agggtggata ttttttaagt gtaataataa tccatgcgca agcgcctgct
    37381 taatgggcgc gtctgtctcc ggaatagacg acatttcgcg aagccatttt tccacacgca
    37441 tacactcttt ccctttaggg aaaattctca gcaaaggcat atggtgttca agaagaatac
    37501 atttgcaatc tgttcgcact gaatcgataa actgcactaa tttcttaagc ccggtaaagc
    37561 gtccagcctg cacatttgct ttaatatcaa gaaacgcatc atcctggtca tatacccaaa
    37621 ctgtcccttt ttgattttca agtatgcgaa aaacaaactt aaccgggtga tcagggtaat
    37681 ccatccagtc ataaaatcca gggaaatcat taaactgcac atttatggtg agtgcgttat
    37741 cgtctaacca ctcacagtct cgtacggcag ccatgagatg aatgagtttg tcgtcatcga
    37801 ctaaatcatc acgacaatca taaggataat cataatattg tttaatcaaa accttgaata
    37861 tatcgacaca ggaaaccagg gagcttttga tatcctctgg taactgattg tttttaaccg
    37921 tattaagtat gctttgttgc gtatatggca tcaaattatt gtgcggagct tttaatgata
    37981 tcatcgcgat
//
LOCUS       DAFAVF010000027        37777 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFAVF010000027 DAFAVF010000000
VERSION     DAFAVF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.027"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..188)
                     /locus_tag="LGK42_001365"
     CDS             complement(<1..188)
                     /locus_tag="LGK42_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBK2657628.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRR"
     gene            complement(263..406)
                     /gene="mgrB"
                     /locus_tag="LGK42_001366"
     CDS             complement(263..406)
                     /gene="mgrB"
                     /locus_tag="LGK42_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBK2657629.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            565..804
                     /locus_tag="LGK42_001367"
     CDS             565..804
                     /locus_tag="LGK42_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657630.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(947..1738)
                     /gene="kdgR"
                     /locus_tag="LGK42_001368"
     CDS             complement(947..1738)
                     /gene="kdgR"
                     /locus_tag="LGK42_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBK2657631.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1915..3288
                     /locus_tag="LGK42_001369"
     CDS             1915..3288
                     /locus_tag="LGK42_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657632.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILTVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3334..4215)
                     /gene="htpX"
                     /locus_tag="LGK42_001370"
     CDS             complement(3334..4215)
                     /gene="htpX"
                     /locus_tag="LGK42_001370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBK2657633.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4407..6455)
                     /gene="prc"
                     /locus_tag="LGK42_001371"
     CDS             complement(4407..6455)
                     /gene="prc"
                     /locus_tag="LGK42_001371"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBK2657634.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6475..7173)
                     /gene="proQ"
                     /locus_tag="LGK42_001372"
     CDS             complement(6475..7173)
                     /gene="proQ"
                     /locus_tag="LGK42_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBK2657635.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7270..7767)
                     /gene="msrC"
                     /locus_tag="LGK42_001373"
     CDS             complement(7270..7767)
                     /gene="msrC"
                     /locus_tag="LGK42_001373"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBK2657636.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7897..9180
                     /gene="yebS"
                     /locus_tag="LGK42_001374"
     CDS             7897..9180
                     /gene="yebS"
                     /locus_tag="LGK42_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBK2657637.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9149..11782
                     /gene="yebT"
                     /locus_tag="LGK42_001375"
     CDS             9149..11782
                     /gene="yebT"
                     /locus_tag="LGK42_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBK2657638.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            11862..13301
                     /gene="rsmF"
                     /locus_tag="LGK42_001376"
     CDS             11862..13301
                     /gene="rsmF"
                     /locus_tag="LGK42_001376"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBK2657639.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13419..13655
                     /locus_tag="LGK42_001377"
     CDS             13419..13655
                     /locus_tag="LGK42_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBK2657640.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13760..13951
                     /locus_tag="LGK42_001378"
     CDS             13760..13951
                     /locus_tag="LGK42_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBK2657641.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(13952..14608)
                     /gene="pphA"
                     /locus_tag="LGK42_001379"
     CDS             complement(13952..14608)
                     /gene="pphA"
                     /locus_tag="LGK42_001379"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBK2657642.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15004..15345)
                     /locus_tag="LGK42_001380"
     CDS             complement(15004..15345)
                     /locus_tag="LGK42_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBK2657643.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15358..16230)
                     /gene="copD"
                     /locus_tag="LGK42_001381"
     CDS             complement(15358..16230)
                     /gene="copD"
                     /locus_tag="LGK42_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBK2657644.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16234..16608)
                     /gene="yobA"
                     /locus_tag="LGK42_001382"
     CDS             complement(16234..16608)
                     /gene="yobA"
                     /locus_tag="LGK42_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBK2657645.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16747..16977
                     /gene="holE"
                     /locus_tag="LGK42_001383"
     CDS             16747..16977
                     /gene="holE"
                     /locus_tag="LGK42_001383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBK2657646.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17079..17735
                     /locus_tag="LGK42_001384"
     CDS             17079..17735
                     /locus_tag="LGK42_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBK2657647.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17759..18421
                     /gene="exoX"
                     /locus_tag="LGK42_001385"
     CDS             17759..18421
                     /gene="exoX"
                     /locus_tag="LGK42_001385"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBK2657648.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18418..20478)
                     /gene="ptrB"
                     /locus_tag="LGK42_001386"
     CDS             complement(18418..20478)
                     /gene="ptrB"
                     /locus_tag="LGK42_001386"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBK2657649.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20687..21346)
                     /locus_tag="LGK42_001387"
     CDS             complement(20687..21346)
                     /locus_tag="LGK42_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBK2657650.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21673..22029)
                     /locus_tag="LGK42_001388"
     CDS             complement(21673..22029)
                     /locus_tag="LGK42_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBK2657651.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22096..22386)
                     /gene="yebG"
                     /locus_tag="LGK42_001389"
     CDS             complement(22096..22386)
                     /gene="yebG"
                     /locus_tag="LGK42_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBK2657652.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22520..23698
                     /gene="purT"
                     /locus_tag="LGK42_001390"
     CDS             22520..23698
                     /gene="purT"
                     /locus_tag="LGK42_001390"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBK2657653.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23754..24395)
                     /gene="kdgA"
                     /locus_tag="LGK42_001391"
                     /gene_synonym="eda"
     CDS             complement(23754..24395)
                     /gene="kdgA"
                     /locus_tag="LGK42_001391"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK2657654.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24432..26243)
                     /gene="edd"
                     /locus_tag="LGK42_001392"
     CDS             complement(24432..26243)
                     /gene="edd"
                     /locus_tag="LGK42_001392"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBK2657655.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26478..27953)
                     /gene="zwf"
                     /locus_tag="LGK42_001393"
     CDS             complement(26478..27953)
                     /gene="zwf"
                     /locus_tag="LGK42_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK2657656.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28291..29160
                     /gene="hexR"
                     /locus_tag="LGK42_001394"
                     /gene_synonym="yebK"
     CDS             28291..29160
                     /gene="hexR"
                     /locus_tag="LGK42_001394"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBK2657657.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29288..30730
                     /gene="pyk"
                     /locus_tag="LGK42_001395"
     CDS             29288..30730
                     /gene="pyk"
                     /locus_tag="LGK42_001395"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK2657658.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(30861..31832)
                     /gene="lpxM"
                     /locus_tag="LGK42_001396"
                     /gene_synonym="msbB"
     CDS             complement(30861..31832)
                     /gene="lpxM"
                     /locus_tag="LGK42_001396"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBK2657659.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(31952..33274)
                     /gene="mepM"
                     /locus_tag="LGK42_001397"
     CDS             complement(31952..33274)
                     /gene="mepM"
                     /locus_tag="LGK42_001397"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBK2657660.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33290..34222)
                     /gene="znuA"
                     /locus_tag="LGK42_001398"
     CDS             complement(33290..34222)
                     /gene="znuA"
                     /locus_tag="LGK42_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBK2657661.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34301..35056
                     /gene="znuC"
                     /locus_tag="LGK42_001399"
     CDS             34301..35056
                     /gene="znuC"
                     /locus_tag="LGK42_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBK2657662.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35053..35838
                     /gene="znuB"
                     /locus_tag="LGK42_001400"
     CDS             35053..35838
                     /gene="znuB"
                     /locus_tag="LGK42_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBK2657663.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(35985..36995)
                     /gene="ruvB"
                     /locus_tag="LGK42_001401"
     CDS             complement(35985..36995)
                     /gene="ruvB"
                     /locus_tag="LGK42_001401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK2657664.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37004..37615)
                     /gene="ruvA"
                     /locus_tag="LGK42_001402"
     CDS             complement(37004..37615)
                     /gene="ruvA"
                     /locus_tag="LGK42_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK2657665.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9060 a         9746 c         9318 g         9653 t
ORIGIN      
        1 aaacgacgta acagtgcatt ttgacgtttt tgctgatcca gcaacgcctc aagcagttca
       61 atctgttcgt tagtccgaga actggcacga ttaataaaaa accacaagat gagcccgata
      121 agaagaacca ccaccgatac aaccaaagac gcaatgttca tcacgcctga atttacaact
      181 tcgttcattt caccacctct gagtagaggc gctattctac cactgctgga gaggaagaaa
      241 atctagtgct gaaaaaatga tatcaccacg ggataaactg gttaatggca caaattccgc
      301 tgaaaaattg tacatcctga tcgcacatca tgttgaatac ctgcgcccaa agcagcaagc
      361 aagccaacac cacgacaacc agaacgaccc atcgaaactt tttcactcca atctccgttt
      421 cacctacctt atgtcattaa actagcatgc gttagttaaa cagcgcctaa ctatgtcgat
      481 attcgtgctt tttcggaatg agtcgcttgc ttcagcgcat attgccgcta cgattaagcg
      541 aacataaaaa gagaagaggt tgtaatgcga ttcatcattc gcacagttat gctgatagca
      601 ctggtatgga taggattatt actcagcgga tatggcgttt tgattggaag caaagagaat
      661 gccgcaggat taggtttgca atgtacctat ctgactgcca gaggcaccag cacggtacaa
      721 tacttgcaca ctaaaagtgg tttcttgggg ataacagatt gcccactgtt acgtaaaagc
      781 aatatcgtgg tcgataatgg ttaagggtaa taaataacca gattctcact catgagtaaa
      841 atattcttat agcaagcgcg tttaatataa cgcaccttca actttatttt tctcatgcta
      901 aataaagaac ctgtctgttt gatattccga caggttctta ctctcatcag aacggatagt
      961 cgtgataacc catttgggca gaaattttgc gcgctgcggt atgcaacatt gcgacatatt
     1021 cctgtaaacg ctcttcagag aaacgcaacg tcgggaagga gatgctcaaa ccggcaatga
     1081 ccacgccaaa gcgatcaaat accggtaccg caatgcatcg cagcccttct tcctgctctt
     1141 cgttatcttc gccatacccc tgctcgcgca cctggtccag aacgggtaat aacgcttctg
     1201 tactggtgat ggtccgctcg gtactgcgtt tatactccac gccctcaaga atttgcttca
     1261 cttcatcgag atcgcgccat gccagcagta ccttaccaat cgcggtgctg tacagcggat
     1321 tacgacgccc aatccgtgaa tacatgcgca aattgtacat agagtcaatt ttgtgaatgt
     1381 aaacaatact gtcttcgtcc agtgcgccga ggtggatagt ttctttggtc aggcgggaga
     1441 tctcacgcat ctggatatct gcgctacgaa ttaaatcgac gttttgtaac gcgcgagcgc
     1501 ccagttcaaa caatttcagg gtcagggaat atttctccga ctccccttcc tgcgccacat
     1561 aacctaaggt tttcatggtc tgtaaaaagc gataaacggt gctttttgac atcatgacgc
     1621 gctgcgacag ctcggttatc cctatttcgc gctcttcacc cagcgcctgc aaaatgccaa
     1681 aaacttttag cacggaagat acagaatcag gctgtttatc cagatctgcg ttagccattt
     1741 atcacctcat tgcgagtgtt ttataaaaat cagaactgtt ttttattata atttcgcacc
     1801 agggtggtcg caatccatct tttgccggtt agttacaatt ctgcgacatc caccgtgaat
     1861 atcagtgcta gaatcatacc cctgttgatt attcaccaaa gatataaaat tcctatgcca
     1921 aaagttcagg ccgacggcct gccattgccc cagcgatacg gtgcgatatt aaccattgtg
     1981 attggtattt cgatggctgt ccttgacggc gcaatcgcca acgtcgccct gccaacaatc
     2041 gccacggacc ttcatgccac gccagccagt tccatctggg tagtgaacgc ctatcaaatc
     2101 gccattgtca tctccctgct ctcgttttcg tttctgggcg atatgtttgg ctatcgacgt
     2161 atttataaat gcggtctggt cgtttttctg ttgtcttcac tgttctgcgc cctttctgat
     2221 tcgctgcaaa tgctcaccct tgcgcgtgtc atacaaggtt tcggcggtgc agcgttgatg
     2281 agcgttaata ccgcacttat ccgcctgatc tatccacaac gttttctggg gagagggatg
     2341 ggcataaact cgtttattgt tgccgtttct tctgctgccg ggccgacaat tgctgcagca
     2401 atcctctcca tcgcatcctg gaaatggtta tttttaatca acgtaccgtt aggtattatc
     2461 gccctgcttc tggcgatgcg ttttctgcca cccaatggtt ctcgcgccag taaaccccgt
     2521 ttcgacctgc ccagcgccgt gatgaacgcg ttaaccttcg gcctgcttat cactgcgttg
     2581 agtggtttcg ctcaggggca atcgctgacg ttaattgctg cggaactggt ggtaatggtt
     2641 gttgttggta ttttctttat tcgccgccag ctttctcttc ccgtaccgct gctaccggtg
     2701 gatttactgc gtatcccgct gttttcactt tctatttgca catctgtttg ctctttctgc
     2761 gcacaaatgc tggcaatggt ttccctgccc ttttacctgc aaaccgtgct cgggcgtagt
     2821 gaagtcgaaa caggtttact tctgacaccg tggccgttag caacgatggt gatggctccg
     2881 ttggcaggct atttgattga acgcgtacat gcaggattgc tgggtgctct agggttgttc
     2941 atcatggctg cggggctttt ttccctggtt ctgctgcccg cgtcacctgc ggatatcaat
     3001 attatctggc cgatgatctt atgtggcgct ggatttggct tgttccagtc acccaataac
     3061 cacaccatta ttacctccgc tcctcgcgaa cgtagcggtg gagccagtgg catgttagga
     3121 acggctcgtc tactgggtca gagtagcggc gcggcactgg tggcgctgat gctaaatcag
     3181 ttcggtgata atggtacgca cgtttcgctg atggctgcgg ctattctgac agtgattgct
     3241 gcctgtgtca gtggtttacg tatcactcag ccacgatcca gggcataaaa aaagcgcgtc
     3301 gatcaggacg cgctttttag tatttacttc atattacttc aggtattcac ccgtacgcag
     3361 agcttcaatt cgtttatcca gcggcgggtg agtcatgaac aactcactga gcgatttcga
     3421 cttaccgtta atgcagaaag ccatcatgct ggttgcttct tgcggttcat agctggtttt
     3481 caggcgctgc agcgcggcaa tcattttctc gcgaccaacc agttttgccg aaccggcatc
     3541 agcatggaat tcacgatgac gcgagaacca catggtgata atgctcgcca gaataccaaa
     3601 caccagttcc agaaccgttg caaccgcaaa gtagatcagc gggttgccgt tgctctcttc
     3661 accttcatca cgatttccgc ccataaaacc cgcggcaagc tgcgccagaa tacgggaaat
     3721 aaagataacg aaggtgttca ccacgccctg aatcagcgtc atggtgacca tatcaccatt
     3781 ggcgatgtgg ctgatttcgt gagcaattac cgcctcggct tcatccgggc tcatgttctg
     3841 cagcaaaccg gtgctgacag caaccagaga ggcatcacgg cgcgcaccgg ttgcaaaagc
     3901 gttgatgtcc ggcgcatggt agatagccac ttgcggcata gcgatccctg cctgacgagc
     3961 ctgggttgct acagtattaa ccagccaacg ttccctttcg ttacgcggtt gctcgatcac
     4021 ttccccgcca acagatcgta atgccatcca tttggacatc agaagcgaaa cgaaggaacc
     4081 accaaaaccg aacagcaagg ccatgatcat cagcccctga acgctgctcg actgtatccc
     4141 tgtcaggctc agtaccagcc cgaaaacgac cattacggcc aggttcgtta gcaggaagag
     4201 cgcgattcgc atcataattt tctttttacc tcagtttaac aaaacgcaat atgcgatacc
     4261 cacatcgtat gggttacgcg actattttca agtctggata gtgcgtaagt caccagaaag
     4321 acacaacttt acattttgta gcatctgatt tacggcatct tgccgctgtt aaaaaatcag
     4381 gcacaatttc ttgtgcctga ttgatattac ttgacgggag cgggttgttc cgcgggtctg
     4441 gctttttcaa gcttcgccag atcgagtgcg atattcaccg tctcatccag ataaggatcc
     4501 ggctcctggt aatctttcgg tagatcatcc agcttcttca actccggttt accttcgcgt
     4561 ttaaagcgtt cgttcaaacg cgccagacgc gtcgcatcat cttcattatt ctctttctca
     4621 cgcacagcgt agttcagaga aacgatattg cgcttgtcct tcatagcgtt gaagcgcgcg
     4681 atatccttca tgatgttctg gaactcagga tctttcgcga tacgcgcatt atgttccttc
     4741 agcagctccg gcccaaaggc cgttaaatcg cctgatttca cataagtcgc ggcatcaatg
     4801 ctatcccacg gcagcgcgtt atcttcgaat ttctcacccg tttccgtttc ttcattaccc
     4861 gtcggcatga tgatgtctgg cgttacgcct ttacgttgcg tactgccgcc gttaacgcga
     4921 tagaatttct ggatcgtgta ctgcacagaa cccagcgctg gccattcagg acgtaacatc
     4981 tgatcgtaaa tacggttcaa tgaacggtat tgctgaacgg tgcctttacc aaacgtcggt
     5041 tcacccacaa ccagcgcacg accgtaatcc tgcattgccg cggcaaagat ttctgaagcc
     5101 gaagcactga aacggtcaac cagtaccacc agcgggcctt tatagaacac ctgcccgtcg
     5161 gtatcgctgt cttcacgaac cttgccgttg ttatcgcgga cctgaacaat gggacccgca
     5221 ggaataaaca gacctgagag cgataccgct tcggtcaacg ccccaccgcc attgctacgc
     5281 aggtcgatga tcacactgct gacattctgt ttttccagtt tctgcagttg aactttgaca
     5341 tcgtctgtca aacccacata gaagcccgga atatccagca cgccgacttt ctctttaccg
     5401 acggtcttca ccgacatttt aaccgcgcgg tcttcgagac gaatacgttc acgggtcaat
     5461 gttacagtgc gggtcttggt ccctttacca gcaggtaaaa tttccagacg aactttactg
     5521 cccttcggcc ctttaattaa ggcaaccaca tcatcaagac gccagccaat cacgtcaacc
     5581 atcggcttgc ctgtttgacc aacaccgaca attttgtcac caacgctgat agctttactc
     5641 ttcgctgccg gaccacctgc caccatcgaa ttgataacgg tgtagtcatc atccatttgc
     5701 agcactgcgc caataccttc cagcgacaaa ctcatttcag tgttgaactg ttcggtatta
     5761 cgcggggaaa gatagttggt atgcgggtcg atttcacgcg caaacgccgt cattgccagc
     5821 gagaaaacat cttcgctgtt ggtttgcgcc agacgacgaa tggcaaattt gtagcggcgt
     5881 gtcagggttt cacgaatttc tttatccgtt tttcctgcca gcttcaggct taactcgtcg
     5941 aatttgactt tactgtccca cagcgcgttc aactcagcct cgtttttcgg ccagggcgct
     6001 ttgctgcggt caaggttata agtgtcgttg ccggtgaaat ccatcggctt ttccagtacc
     6061 gacaaagcgt actggtaacg ctcaaaacgg cgcttttgcg ccagattgta gagatcgtag
     6121 aaaacgtcga gtttgcctga acgcagttca tcgcctaact cggttttctt tttcgcgaac
     6181 tgttcaacat cgcttgccag cagcacgttg tggctgtaat cgagcagatt caggtagcgg
     6241 tcaaagattt tggccgaaaa tgcctgatcg aggtcgaact ggcgataatg agaacgggtg
     6301 aagcgcgacg ttacgcgctc acttaccgtc gcatgctgcg tctcttcctt taataccgga
     6361 atttgatcag cacgcgtgat atcttctaca gcgaaggtct ggcctgctat tgcaagcagg
     6421 ccagctaacg cggtaagcct aaaaaacatg ttcatgcctg gcccggcctc cgtttcagaa
     6481 caccaggtgt tctgcgcgca caatcaaaga catacccgaa ttcagctgga cgcggacgcc
     6541 gtctttggtg atttctaata cggtggcatc catcgcgttt tgacccgctt tcaccttcag
     6601 ggcttgtccg acagtcagag ctgaaatgtc agaaaccggg gtgtgctgtt cttcgcgagg
     6661 tgcttttact gtttttggcg ctttctctac cggcttttgc gcacgaggtt tacgttcagc
     6721 gccttctttg cggcgtggcg tggtcggacg tggcttgcgt tcgcggcgcg gtgcgtcttc
     6781 tttctcacca gcagctgcgg cagcttcgcg ttttttcgct tgctgttcag cacgctgtgc
     6841 ctgaacacgc gctttcgctt cttcaagctg cttgcgagca tgctctacat gttgctcgtc
     6901 cagctcaccg catgggttgc cgtcaagatc gacacgcgtt gcgccgggtt taacaccgta
     6961 aagataacgc cagctcgaag tgtagagacg taaagcggat cgtaattgcg ttttgctcag
     7021 gttcatttcc ccggcaacac gatcgaccaa atcctgaaaa ataccgattt tcagcggacg
     7081 cgcttcacct tccgcactga aacagtgggg aaaacgttcg gccagaaacg cgattacttc
     7141 tttactgcta ttcaacttag gttgattttc catgaaattt cctgattaca acggacgtag
     7201 ccaacaagcg caggcatgaa caggcgtcat tataatgacg ctaccagtaa atgctacgtt
     7261 atccgttgat tatcctgcga cgctcgcaaa gaattttttg taatccgtcg ttgcaagcac
     7321 tttttcaagc tgtgccacaa gctgacgtaa gccttgctcg tcctcgtctg taaagcgacc
     7381 gaagacggta ctatcgatgt caagaacacc aataatctga tttttcacca ccagcggcag
     7441 aacaatttca gaattactcg ccgcatcaca ggcaatatgc ccgtcaaacg cgtgaacatc
     7501 ctcgatacgc tgcacttgat tgcgggcaac cgcagtgccg cacaccccgc gcccgacggg
     7561 tatccggaca caggcaattt tgccctgaaa tggtccgagt accagtgtat cgtcctcaag
     7621 caaataaaaa cctgcccagt ttacgtcagt gagacgctcg tataacaacg cactggtgtt
     7681 cgcaagcgtt gccagaaaac tggtttctcc cgccatcagc gcgttaaagt cgcgatttaa
     7741 atccgcgtaa aattctgttt tgttcattat ataatcactt gtttgtctta cctggaactg
     7801 ccagcctatt aaaataagca ttaaatgcgt taatgctcaa gatcattccc atcatgggtt
     7861 aagattaatg ttaattctta ttacatttgg cacgtcatgg ctcttaacac accacaaatt
     7921 acgccgacaa aaaagataac agtgagggca atcggcgagg aactgccgcg tggtgattac
     7981 caacgttgcc cgcaatgtga catgctgttt agcctgcccg agataaattc tcatcagagt
     8041 gcctattgtc cgcgctgtca ggcaaaaatt cgcgacgggc gcgactggtc gctaacgcgc
     8101 ctggcggcaa tggctttcac catgctgttg ctgatgccgt ttgcctgggg cgaaccgctg
     8161 ttgcatatct ggctgttagg tattcgtatc gacgccaacg tcatgcaagg catctggcaa
     8221 atgaccaaac agggcgatac gataacgggg gcgatggtct ttttctgcgt tattggtgcc
     8281 cccctcattc tggtgtcctc catagcatat ttatggtttg gtaaccgact gggaatgaat
     8341 ttacgtccgg tactgctgat gcttgagcga cttaaagagt gggtaatgct ggatatctac
     8401 ctggtcggca ttggcgttgc ttctataaag gtacaggatt atgcccatat ccaggcgggt
     8461 gtcggcttgt tctcttttgt ggcgttggtg attttaacga cggtgacgtt gtcacatctt
     8521 aatgtcgagg agctgtggga gcgattttat ccgcagcgcc ccgctacgcg tagggacgag
     8581 aaacttcgtg tctgtcttgg gtgccatttt accggctatc ctgatcagcg tggtcgctgc
     8641 ccgcgttgcc atatcccgct acgcctgcgt cgccgtcata gtttgcaaaa atgctgggcg
     8701 gcgctgttag cgtcaatcgt tttgttgtta cctgccaacc tgttgcctat ttctatcatt
     8761 tatctgaacg gaggacggca ggaagataca attctttccg gaattatgtc gctggcaagt
     8821 agcaacattg cggttgcggg aatcgtgttt atcgccagta ttctggtacc gtttactaaa
     8881 gtgatcgtca tgttcacttt actgttgagc attcatttta aatgccagca aggtttacgc
     8941 acacgcattc tgttactgcg gatggtgacc tggattggtc gctggtcgat gctcgacctg
     9001 tttgtcatat ctttaaccat gtcgctgatt aatcgcgatc agatcctcgc ttttactatg
     9061 ggaccggctg cgttttattt tggcgcagcc gtaattttga ctattcttgc tgtggaatgg
     9121 ctggacagcc gcttactttg ggatgcacat gagtcaggaa acgcccgctt cgacgactga
     9181 agcgcagatt aaaaataaac gccgtatctc acctttctgg ctgctgcctt tcatcgcgct
     9241 aatgattgcc ggttggctga tttgggacag ttatcaggac cggggtaata ccgtcaccat
     9301 cgactttatg tcggcggatg gtattgtccc gggccgtacg cctgttcgtt atcagggcgt
     9361 tgaagtcgga acagtgcagg atatcagcct cagcgacgat cttcgtaaga ttgaagtcaa
     9421 ggtcagcatc aagtccgata tgaaagatgc gctgcgcgaa gagactcaat tctggctggt
     9481 gacgccaaaa gcatcgttgg caggtgtctc tgggctggac gccctcgtcg gtgggaacta
     9541 tatcggcatg atgccgggta aaggtaaaga gcaggatcac tttgtcgcac tcgataccca
     9601 accgaaatat cggctggaca atggcgatct gatgatccac ctgcaagccc ccgatctcgg
     9661 ttcgctgaac agcggttcat tggtctattt ccgcaagatc ccggtgggaa aagtctacga
     9721 ctatgccatc aatcccaaca aacaaggcgt ggtgattgat gtcctgatcg agcggcgttt
     9781 taccgatctg gtgaaaaaag gtagccgttt ctggaacgtt tccggcgttg atgccaatgt
     9841 cagtatcagt ggcgcgaagg tgaaactgga aagcctggcg gcactggtta acggtgcgat
     9901 tgccttcgat tcaccagaag agtcgaaacc tgccgaggcg gaagatacct ttggtctgta
     9961 tgaagacctc gcccatagcc agcgtggcgt aataataaaa ctggaactgc cgagtggggc
    10021 cggattaacc gccgactcga cgccattaat gtatcaggga ctggaagtcg gacagctaac
    10081 taaactggat ttaaatcctg gtggtaaagt cactggggaa atgacagttg atcccagcgt
    10141 cgttaccctg cttcgtgaaa atacccgcat cgaattacgt aatccgaaat tatcccttag
    10201 cgatgccaat ctcagcgccc tgctgactgg caaaaccttc gagttggtac ccggcgatgg
    10261 cgagccacgc aaagagttcg ttgttgtgcc aggcgaaaaa gcactgctgc atgaacctga
    10321 tgttctgacg ctgaccctga ccgcgccgga aagttacggt attgatgctg gtcagccgct
    10381 cattcttcac ggcgtgcagg taggccaggt tatcgatcgt aaactcacca gcaaaggcgt
    10441 cacctttacc gtcgccatcg agcctcagca tcgagaactg gtaaaaggcg atagcaaatt
    10501 tgtcgtcaac agccgtgtcg acgtgaaggt ggggctggat ggcgttgagt ttctcggtgc
    10561 cagcgcctca gaatggatta acggcgggat acgtattctg ccgggcgata aaggcgagat
    10621 gaaagccagc tatccactgt atgccaatct ggaaaaagcg ctggagaaca gccttagcga
    10681 tttacccacc acaaccgtga gtttgagtgc agagacgctg ccggatgtgc aggcaggatc
    10741 ggtagtgctg taccgtaaat ttgaagttgg tgaagttatt accgtgcgtc cgcgagctaa
    10801 cgcgtttgat atcgatctgc atattaagcc ggagtatcgc aaccttctga ccagcaatag
    10861 cgtgttctgg gcagaaggcg gggcgaaagt tcagctgaat ggtagtggcc tgaccgtaca
    10921 ggcatccccg ctctccagag cattaaaagg ggctattagc ttcgataacc tcagtggtgc
    10981 cagcgccagt cagcgtaaag gcgacaaacg tattctgtat gcttccgaaa cagcggcccg
    11041 tgcggttggt gggcagatta cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat
    11101 gccaattcgc tatctcggta ttgatatcgg gcaaatccag acgctggatc tgattaccgc
    11161 gcgcaatgaa gtacaggcaa aggcggtgct ctatccggaa tatgtccaga cctttgctcg
    11221 cggtggtacg cgcttctcag tggtcacacc gcaaatttcg gcagctggcg ttgagcatct
    11281 tgatactatc ctccagccgt atatcaacgt cgaaccaggc cggggcaatc ctcgccgcga
    11341 ctttgaatta caagaggcca ccattactga ttcgcgttac ctggatggct taagcattat
    11401 tgttgaagcg ccggaagccg gttcgttagg tattggtacg cctgtgctgt tccgtggtct
    11461 ggaagtcggt acggttacag gaatgacgct ggggacattg tcagatcgcg tgatgattgc
    11521 gatgcgcatc agtaaacgct atcaacacct ggtgcgtaac aattccgtct tctggttggc
    11581 atcgggttac agtctggact ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa
    11641 ccagtttatc cgtggcggca tcgccttcgc cacgcctccg ggtacgccac tggcaccgaa
    11701 agcccaggaa ggcaaacact tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg
    11761 gggaactgcg cttcccaaat aatgcccact gctccggcgt gcctgcgccg gagcgtttat
    11821 gctaaactgc gcgcctgttt ttttgccagt ggtacatgct cgtggcccaa cacaccgttt
    11881 atttcccgga cgcctttctg acacaaatgc gcgaagcgat gccttcgacg ctctcatttg
    11941 atgattttct tgccgcctgt cagcgcccgt tgcgccgcag cattcgcgtt aatacgctga
    12001 aaatctccgt tgctgatttc ctgcaattaa ccgctcctta tggctggacg cttacgccaa
    12061 ttccgtggtg tgaagaaggt ttctggattg aacgcgacaa tgaagatgca ttgccattgg
    12121 gtagtaccgc cgagcattta agcggcctgt tttatattca ggaagccagt tcaatgttgc
    12181 ccgttgccgc cttgtttgct gacgataatg caccacagcg ggtgatggat gtcgcagctg
    12241 cgccaggctc caaaacgacg caaattgccg cgcggatgaa taacgaaggg gcaatccttg
    12301 ccaatgagtt ttccgccagt cgggtaaaag tgttacatgc caatatcagc cgctgtggca
    12361 tcagtaatgt tgcgctcaca cattttgatg gccgcgtgtt tggtgcggca gtgccagaaa
    12421 tgttcgatgc cattttgctg gacgctccct gctctggcga aggcgtggtg cgtaaagatc
    12481 ccgatgcgct aaaaaactgg tcaccagaaa gcaatcagga aatcgcagct acacaacggg
    12541 agcttatcga cagcgccttt catgcattac gtcctggtgg tacgctggtt tactcgacct
    12601 gtaccttaaa ccaggaagaa aacgaagccg tttgcctgtg gctgaaagag acttaccccg
    12661 acgcagtaga gtttttacca cttggcgatc tcttccctgg tgcaaacaaa gcgctgaccg
    12721 aagaaggctt tttgcatgtt ttcccacaaa tttacgactg cgaaggcttc ttcgttgctc
    12781 gtctgcgtaa aactcaggcg attcccgcct tacccgcccc caaatacaaa gtgggcaatt
    12841 tcccgttcag cccggtgaaa gatcgcgaag ctggacaaat tcgtcaggcg gctgcaggtg
    12901 ttggcttaaa ctgggatgaa aacctgcgcc tctggcagcg tgacaaagaa ctgtggttgt
    12961 tcccggtggg cattgaagcc ctgatcggta aagtccgatt ttctcggttg gggattaaac
    13021 ttgccgagac gcacaacaaa ggttatcgct ggcagcatga agcggttatt gctcttgcct
    13081 cacccgataa tatgaatgca ttcgagctga caccgcagga agcggaggag tggtatcgcg
    13141 ggcgcgatgt ttacccgcaa gccgcgccag tggcggatga tgtattggtt actttccagc
    13201 atcaaccgat tggtttagcc aaacggattg gttcgcgatt gaaaaacagc tatccgcgtg
    13261 aactggtgcg cgatgggaaa ctttttaccg gtaacgcctg acagcgcaca aaaaaagcgc
    13321 actttttgac tggcacattc ggctgcctca actaggctga aaaatggtgc gatcggactg
    13381 gtcgtaccac aatcggcagc taaatggaga gcacgaagat gaaaaccagt gtgcgcatag
    13441 gcgcttttga aatcgacgac ggcgaattac acggtgaatc gccaggtgat cgaacgttaa
    13501 ccattccttg taaatctgac cccgatttat gtatgcaact ggatgcctgg gatgctgaaa
    13561 ccagtatccc tgccctgcta aatggcgaac actctgtcct ttaccgtacc cgttacgatc
    13621 aacaatctga tgcctggatt atgcgtcttg cctgatccaa aaagaacccg tcggcatggc
    13681 gggttatttg tcctggttat tcccccgttg taaaatctct cctaaactta acggtacggc
    13741 accacacttc ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc tggatggcgg
    13801 ttgtgaagat attgttgtgg atgtctacaa cacggaacag cagtgtcttt attctatgag
    13861 cgatcaacgg atccgccatg gcggttgttt tccgattgag gattttatag atggtttctg
    13921 gcgaccagca caggagtatg gtgattttta attattgcaa ttgcacaaga gtcagttcgc
    13981 ccccaaagac agcaccggta tcaatataat gcaggttgcc aatatccacg cgatgtcgca
    14041 acggtgtatg accaaaccag aaatgatcag cacctgtaat tccctgccct ttttggcgtt
    14101 cacctaatcg cgagcggctc cacaagacct gatgcaaatc aacgtccttt tgccattcat
    14161 aaacatcatc tggataatcg gcatgagcaa taacatgttt gccggtacga ctgtgtactt
    14221 caagaataaa gggcaaatgc tgacattttt ccagcgccgt tttcgcttgt ttctgttgat
    14281 tatctgccag cgcaataaac cagtcgccgc cattcatcaa ccacaaagac atctgctggg
    14341 atgccagcgc atccatcgcc atctgttcat gattgcctct taccgcacaa acccaatgtt
    14401 gttccagtaa ctgcagacaa cgtaaacttt gcggcccacg atcgataacg tctcctactg
    14461 agataagtaa atctcgccac ggatcaaaac gacaatgcca taatttgcgg cgcaactgct
    14521 caagacaacc gtgtatatcg ccagaaagcc agatatgtcg ccattgatga cccgcaattc
    14581 tctgataaac gggcgcaggc tgtttcatca atattttcct cccgcgctaa agatcacata
    14641 atcttaacaa gaatgttaaa aaacgctgga ctcagacagt agagtgtgtg ttatggttga
    14701 ctataaagtc agcgaaggaa atgcttctgg cttttaacag ataaaaagag accgaacacg
    14761 attcctgtat tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc
    14821 attaatgcag gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag
    14881 tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc agctgaaatg ttaaaaaccg
    14941 cccgttctgg tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg
    15001 taattaacag agtttttcag ctcgttctat aaacggtgcc agactcattt tttcgccggg
    15061 attgttagga tcatcaatct gaatcaccga aatgggttgg gctttagtct tcccactggc
    15121 aacttccttt tgtgcgatat cgtttaaagg atactgcacg agggtacttg ggttgatgac
    15181 atacaaagca ttacccggtc ggcaagtcag catcacctct tcgcgattaa acgcccattt
    15241 gtctttaccc acttcaaaac ggctgacggt aatcacctgc ggtgcagcca gcgccgctgc
    15301 agaactggtg agtaacagaa acgccagaat actttttttc atcattttcg taatccatca
    15361 aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca
    15421 ttcgagttta gtcatccaca caaaccaggg tgccgcacga tcttcatcct gcctcatgcg
    15481 cggtacaaga acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc
    15541 tttgagcaat aagagttggc cccagtaagt cgttagcgtg gggggaaatc cggtaatcaa
    15601 caatgcatta agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga
    15661 aaaacgcatc agcgcctgaa tagcctggtg tcgccaacgg cctttgatga gttgcataca
    15721 ccagagcacc ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg
    15781 attagtctga tggattttcg ccgttacccc ttcattcagc gtcgcatgcc cgattcccgc
    15841 cagcagaata aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg
    15901 cataagggcg acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc
    15961 aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa aatacatctg cccatcccgt
    16021 tcccatcaac ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac tccagacggc
    16081 agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag cgacgaatcg ttaagggggc
    16141 cagccaggca ccgtacattg cgaagccaaa gaccagcatc agcgaggtga aatggataaa
    16201 tcgcagcgcg atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc
    16261 tttcgtttta tgcccgtcca ccgaaacaac atgccagtca acgatgtagg ttccgggttt
    16321 cagcgaatcg gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg
    16381 taatgtttta atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc
    16441 aacaccttcc gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc
    16501 cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt
    16561 ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt gaagtcatat tatctattcc
    16621 tttttgtaat aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc
    16681 aattccggct tgccatcctg gctcactctt agtaactttt gcccgcgaat gatgaggaga
    16741 ttaagaatgc tgaagaatct ggctaaactg gatcaaacag aaatggataa agtgaatgtc
    16801 gatttggcgg cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa
    16861 gcggttgaac gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc
    16921 caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac
    16981 ttatacagag ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg
    17041 cacccataat ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag
    17101 cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat catctggagt
    17161 ttgtgagggc cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac
    17221 tggggtgtga ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc
    17281 cgctttgtta tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat
    17341 ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga
    17401 tctaccatca gagtcatggt gcatgtctgg gtcgtcgttc cagaaccatc acggtggtcg
    17461 atgaacaacc ggaaggtatg gatatggacc cgacctgttc actgtttact acagggcaat
    17521 gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt
    17581 actcgattgc cgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg
    17641 gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac
    17701 agggttggca aggcgatatc attccattac gctaggcttt ttcggcctgg agcatgccat
    17761 gttgcgcatt atcgatacag aaacctgcgg tttgcaggga gggatcgttg agattgcctc
    17821 tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg
    17881 tcctattagt ccacaagcga tggcgattca tcgcatcacc gaagccatgg tcgccgataa
    17941 accgtggatt gaagatgtga tcccacacta ttacggtagt gaatggtatg tcgcgcataa
    18001 cgccagcttt gaccgccgcg tactgcctga gatgcccggt gagtggattt gcactatgaa
    18061 actggcccgt cgtttgtggc ctgggatcaa gtacagcaat atggcgttat ataaaacacg
    18121 caagctcaat gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt atgactgtta
    18181 tatcaccgcc gcgttgctta tcgatattat gaacacctcc ggctggacgg cagaacagat
    18241 ggccgatatc accggacgtc cgtcgttgat gacgaccttc acctttggca aataccgtgg
    18301 caaagcggtt tccgacgttg ccgaacgcga tccgggctat ctgcgctggt tatttaataa
    18361 cctggacagc atgagcccgg agctgcgttt aacactgaaa cattatctgg aaaatactta
    18421 gtccgcaggc gtagcgggta atgttccctg cgccagcgcg accagaaaag catattccat
    18481 cgctacgcct tcgtacgatt taaagcgacc agatttaccg ccatggcctg agtccatgtc
    18541 ggtacagagc aataaaagat ggtcatcggt tttcagctcg cgcaatttag cgacccattt
    18601 tgccggttcc caatattgca cctgagaatc gtgcaaaccg gtcgttacca gtaaatgcgg
    18661 ataagcctgt gcggtgacgt tgtcatatgg gctgtagctt ttcatgtact cgtaatattg
    18721 cggatcctgc ggattccccc actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc
    18781 aagcatcgtt gttacaacat caacaaacgg tacctgggcg ataacgccgt ggaataattc
    18841 cgggcgttga ttaattgcaa cgcccatcaa catgcccccc gcactcccgc ccatcgcata
    18901 acaaagcgaa ggagagccat agcccagttt taacaatgca tcgcaggcat caagataatc
    18961 attaaacgta tttttctttt tcagaaattt tccgtcttcg taccattgtt gccccagctc
    19021 accaccgccg cgaacatgga caatggcgta gacaaagcca cgatctaaca aactcaagcg
    19081 gctaaaactg aaatcggcat caatacttgc gccgtaagaa ccatagccat acaccagcaa
    19141 cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc aacgaaaccg gaacttcgac
    19201 gccatcacgg gcgactatcc acaggtgttc actgcggtaa ttcgccgcat caaaaccagg
    19261 aacttccgtt tgttttaata cacgacgctc accggtatcc atatccagtt caaacaaagt
    19321 atctggtgtg gtcatggaag aataaccata acgcaatcgc gcggtttcag gttctggatt
    19381 gtaggcaatc caggtcacat aggccggatc atcaaaggca ataccaatga cttcccgggt
    19441 cttgcggtta atttggcgca aactggttaa cccgcgctga cgctcttcaa ccaccagcca
    19501 gtcggtaaac agcgtaaacc cttccagcat aatgttatcg cgtggcggaa ttaactcttc
    19561 ccactgttgc tcatcacgca ggcgggtacg gtataagcca aagtttttgc catggcggtt
    19621 ggaacgcaga taaaaccgat gctggtagtg atcaaggctg tattcgtgat ctttgcggcg
    19681 cggcagaaaa acaaacggct cggcatcggc catttccgcg tccagcaggc gaacttcact
    19741 ggtggtggcg ctggccaaat gaatgactac atagtgcttc gaggtcgttt tatgcaggct
    19801 gacgtaatag gtatcgtctt tttcttcgta gatcagttta tcttgcgatg ctggcgtacc
    19861 gatggcgtga cgccagacct gataaggcag cagcgtcacc ggatgcttgc gaacatagta
    19921 gaaagtccag gagtcatttg cccagacaaa gctgggttca acgttatcca gcagttccgg
    19981 gtaccagtta ccagtttcca gattacgaaa acgaatgccg tactggcgtc gggaaagaaa
    20041 atcttctgcc agcgccataa tggtgttatc gggcgtaatc gccattccgc ccatcgaata
    20101 aaactcacta tgagctgcgc gcttattggc atcgagcaat gtttcccact catcccactc
    20161 ttcactgaat gccgattgac gctggtagat agcatattca cagcctggtt cataaatatg
    20221 ccgatagcgg tagccatttt tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc
    20281 gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt gaggccatca cccgatgacc
    20341 gtaactattt tcttgttgca ggtagtccag gacttctggc tgagaacgcg tatcgtcccg
    20401 cagccagtag taattatcga tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg
    20461 ggcggctttt ggtagcatgt tattgttctt tctggttgaa acatcttata agggtggcaa
    20521 aactcaccgg ggatgcaagc gaaacagggg agtcattgct tagatgatga caggtaatgg
    20581 cgcggatatc gaatgttatg cagacagaga aaatcagcct gttcaaatgg ctgtgcgatt
    20641 ctggatagcc cgaaatagtc aacttcaggc tatccagaga gcggaattat tccgccaaag
    20701 tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg aacatcctgg gggattttca
    20761 gcgcgtcacc cagtgcattc aggtaactgc gttccataaa atggtcaata tcaatagccg
    20821 cgcaactcag gaaatagatt tccagcgcct cttcttcatt gcggaccccg gtagccaggc
    20881 gttgtggatc cagcggttgt tcgattgcct gctcaatgag tacacgcccc tgctcttcca
    20941 cgccggcttc acgcaattgc tggtcgattg ccgcacgttc tttggcatca atatgaccat
    21001 cacttttagc ggcaaagacc agcgcaagga tcaaacgttc tgtacgctca tccagcggcg
    21061 tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt
    21121 tattccacag caccgtaccc gctaccgctc cgccgccaac cagtaacgcg ttggtgccat
    21181 atttcgtaag aagtttacgt gctgatttat ttgcgaccag cagcccagcc agtccgccta
    21241 atgcgcctgg cactaacagt ttgcccaatc cctgatccgc agacgaggag gtagaagaac
    21301 tgctttgccc aagcagggat tgcagttgat ttaaccagtt agccatattt gctcctcaat
    21361 aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta tatcgagaca
    21421 aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc gccaggtaag
    21481 gtatttactg tatatcaggt gataagggta ttttaggtga aagtagtttg tcgttttgag
    21541 tggcattgtt tgatgctgat tgcccgatgc gacgctgttg cgtcttctca ggcctacgat
    21601 tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat ccgccaacgg tcaatcgaat
    21661 tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt ccacgctgac
    21721 ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc atttatcatc
    21781 tttaccctga atgtcctgca aactgaccca agccacggga tcggcctgac cgacaatttt
    21841 ttgatcatct gcccagcgcg ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc
    21901 aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc tggtttcatt
    21961 attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa acgcccctct
    22021 ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta caaattgtca
    22081 ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac
    22141 gacctgtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc
    22201 tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaatttgt
    22261 cagccaggtg tcaagcagat ccgccgtatc aagcatttta tcataagcat cggcttcctt
    22321 tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac
    22381 cgccatttgc agcctctcat aataactgtg attttataca gtatatttct tttcggttga
    22441 gaaatcaaca tcagcaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat
    22501 taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg
    22561 cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct
    22621 cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca
    22681 tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga
    22741 aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact
    22801 tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg
    22861 cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg cccacttcca cttatcgttt
    22921 tgccgatagc gaaagccttt tccgcgaggc ggttgccgcc attggctatc cctgcattgt
    22981 aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgttctg cagagcaact
    23041 tgctcaggca tgggaatacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt
    23101 tgaaggcgtc gttaagtttg acttcgaaat taccctgcta accgtcagcg cggtggatgg
    23161 cgtccatttc tgtgcaccag taggtcatcg ccaggaagat ggcgactacc gtgaatcctg
    23221 gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt
    23281 ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga
    23341 ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc
    23401 tcaagatctc tcagagtttg ccctgcatgt acgtgccttc ctcggacttc ctgttggcgg
    23461 gatccgtcag tatggtcctg cagcttctgc cgttattctg ccacaactga ccagtcagaa
    23521 tgtcacgttt gataatgtgc agaatgccgt aggcgcagat ttgcagattc gtttatttgg
    23581 taagccggaa attgatggca gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt
    23641 tgttgacgcc attgaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac
    23701 ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg ggcgttttga cttttacagc
    23761 ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc
    23821 agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg
    23881 tagtcacggt agttagccgg agaaatacca cccgtcgggc agaaacggac ctgggagaac
    23941 ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg
    24001 aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga
    24061 attagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta
    24121 attgcgaact gtgcacccgc ttcagtgact tctgccagct gctgtggatt cagcaccgta
    24181 ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca
    24241 cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc
    24301 atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc
    24361 aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca
    24421 aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca
    24481 cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg
    24541 tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt
    24601 ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct
    24661 tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag tcgtccatcg
    24721 gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta
    24781 tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg
    24841 cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc
    24901 gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg
    24961 cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa
    25021 gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtccag ttcaccatta
    25081 ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct
    25141 tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc
    25201 tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca
    25261 tcagaaaggt cggagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc
    25321 accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagagcaac aataccgttc
    25381 accaccactt tctcatcgat catcttaccg atcggcatcc attcattgcc attaccggtc
    25441 atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga
    25501 tgaacaaaag aagagcctgg caactgcatc cccataaatt ccaccaccat ctggttggtg
    25561 ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc
    25621 agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct
    25681 ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca
    25741 aacgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga
    25801 gcaccatcaa acatgttatg ggacagcccg accgccgcag acatcgctat cacttcgcgg
    25861 cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga
    25921 acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct
    25981 ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg
    26041 atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa
    26101 ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgaagt tttcgcttgt
    26161 tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt
    26221 acgcgtaaca attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat
    26281 tcacaacgcg tttcattcag aggatttatg actgaaacgc ctgtaaccgg agctcatagg
    26341 gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct
    26401 gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata
    26461 aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc
    26521 gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc
    26581 atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac ttcgtcgcga
    26641 cgaacaaaca gtgcctgaat accacgcatg gtttccagca gcaaacgttc ataggcatcc
    26701 gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg
    26761 ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca
    26821 ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgattc tttaaacaga
    26881 ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta
    26941 ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc
    27001 gctacgaagg tttctgtatt gctgctcttg ttcgcgccct cttcttccag atatcccggc
    27061 acttttttgc cctgggcgaa gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg
    27121 ttggagcggt cgatgcggcg cagagacttc agtactttca ctttttcatc gcggatgctg
    27181 tctgcgctca ggtcagacgg cggagacatc gcaatcatgc agagaatttg cagcaggtgg
    27241 ttttggatca tatcgcgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc
    27301 cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc
    27361 acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga
    27421 tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt
    27481 tcctgcgagg tcgccagcga cgtccccagc ggtttctcca taactacgcg tgccggttta
    27541 gcattcagtt ttgcctcacc aagccctttg caaattgcgc caaaagtgct gggcggcatg
    27601 gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag acggctgaat
    27661 gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat
    27721 aaaccttcat caatagtttc tttcatgaaa gtttcgagcg cctcacggac aactttggta
    27781 taagccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc
    27841 tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct
    27901 ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc catgtcattc
    27961 tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc
    28021 actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg
    28081 ggaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc attcggtaaa
    28141 aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt
    28201 aacacggcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt cagtgcggaa
    28261 atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat ccagtctcag
    28321 ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat
    28381 aacgcgatcc attcgagtat tgctgctatg gcactggaag ccaatgttag cgaaccgacg
    28441 gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg
    28501 gcacagagtc tggcgaatgg cactccctat gttaatcgca acgtcaatga agatgacagc
    28561 gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgctcga tcatgtccgt
    28621 cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa
    28681 atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc
    28741 tttcgtttta atgttccggt ggtgtactcc gatgatattg tgctgcaacg catgagttgt
    28801 atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat
    28861 ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct
    28921 gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat
    28981 actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg
    29041 gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag
    29101 gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa
    29161 aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc
    29221 gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc agtcaacgga
    29281 gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac
    29341 agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa
    29401 cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc
    29461 cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt
    29521 atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc
    29581 caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc
    29641 tgctgacgtc gtgcctggtg acatcctgct gctggacgat ggtcgcgtcc agttaaaagt
    29701 actggaagtt cagggcatga aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa
    29761 caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga
    29821 caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc
    29881 acgctgtggc gaagatctga actatgcccg tcggctggca cgcgatgcag gatgtgatgc
    29941 gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga
    30001 catcatcctc gcctctgacg tggtaatggt tgcacgtggc gacctcggtg tggaaattgg
    30061 cgacccggaa ctggtcggca ttcagaaagc gttaatccgt cgtgcgcgtc agctaaaccg
    30121 agcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg
    30181 tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc
    30241 tgcagaaact gccgctgggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg
    30301 cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt
    30361 cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg
    30421 cgttacggcg atcattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat
    30481 cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc
    30541 tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc
    30601 cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg acctggtgat
    30661 tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac
    30721 ggtagagtaa gtacgttgcc ggatgcggcg aaaacgccac atccggccta cagttcaatg
    30781 atagttcaac agatttcgaa tattctgaag caaacttgaa cttatcatca ggcgaaggcc
    30841 tctcctcgcg agaggctttt ttatttgatg ggataaagat ctttgcgctt atacggctgg
    30901 atttcgcccg gtttgcgagt tttcagcaat tttagtatcc aggtgtattg ttctggtcgc
    30961 ggaccaacaa aaatctcgac ttcttcattc atccgccgcg caatcgtatg atcatccgcc
    31021 tctaacagat catccatcgg tgggcgcacc tgaatcgtca gacggtgcgt cttgccatca
    31081 taaatcggaa acagcggaac aacgcgcgca cggcacactt tcatcaaacg accaatcgcg
    31141 ggcaacgtcg ctttataggt ggcaaagaaa tcaacaaatt cgctgtgctc agggccatga
    31201 tcctgatcgg gtaaataata tccccagtag ccctgacgta ccgactggat gaatggttta
    31261 ataccatcat ttctcgcatg cagacgaccg ccaaaacgac gacgcaccgt gttccagaca
    31321 taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg ccattttctg cccttgcgag
    31381 gccatcagca tggcaggaat atcgacggcc caaccgtgcg gcaccagaaa gataactttc
    31441 tcgttattac gccgcatctc ttcaatgatc tccagccctt gccagtcaac gcgcggctga
    31501 attttctccg gcccgcgtat tgccaactca gccatcattg ccatcgcttg cggcgcggtg
    31561 gcaaacatct catcaacaat cgcttcgcgt tcagcttcac tacgttctgg aaagcagagc
    31621 gacagattga ttaacgcacg acggcgtgag ctttttccca gtcgtccggc aaaacgtccc
    31681 agccgtgcca gaatgggatc acggaacttt ggcggcgtta aagcgatacc cgccatcgct
    31741 gctacgccca gccatgctcc ccagtagcgc gggtggcgaa aggatttatc aaactcagga
    31801 atgtattcgc tattattttt tttcgtttcc atgcttttcc agtttcggat aaggcaaaaa
    31861 tcaatctggt gatagtgtag cggcgcaact tgccccgcac caaataaaaa aagccggtac
    31921 tgactgcgta ccggctgcga atggatgtta attaatcaaa ccgtagctgc ggcacaatct
    31981 ctttggcctg cgccaggaat tcgcgacgat cggagccggt cagcccttcg gtacgcggca
    32041 gttttgccgt cagcgggttt acggcctgct ggtttatcca tacttcatag tgcagatgtg
    32101 gcccggttga acgtccggta ttaccggaaa gcgcgatacg gtcgccacgt ttcaccttct
    32161 gtcccggttt caccagaatc ttgcgcaagt gcatataacg cgtggtgtag ctgcgaccat
    32221 gacgaatagc cacataataa cctgctgcgc cactgcgttt ggcaaccacc acttcaccgt
    32281 cacccactga aagcactggc gtaccctgcg gcatggcgaa atcaacacct ctgtgtggtg
    32341 caacgcgacc ggtcacagga ttagtacgac gcgggttaaa gttagacgag atacggaact
    32401 gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc agtaccgtta cgatcgtaga
    32461 atttgccatc ttcagcgcgg atagcgtaat aatctttacc ttctgaacgc aaacgtacgc
    32521 ccagcagctg gctttgctca cgtttaccat caagcatttc tcgagacatt aataccgcaa
    32581 attcatcgcc ttttttcagt ttgcggaaat ccatttgcca ctgcatggct ttaatcactg
    32641 cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct ggcaacaaag ctccccccga
    32701 cggtaccttt cagcagattg ttaacccact ctccttgctg catttcgctg gtcattttaa
    32761 aaccgttagc ggcagtacgg tcataggttc gggtttcacg acgagacact tcccaggtga
    32821 ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag ttgttgaccg attttcaggt
    32881 tacgcaattc tttgtcggca gcagccagtt gggtgatatc acccatatca ataccatact
    32941 gattgagaat gctgcttagc gtatcgccag tggaaacaac atattcatgc acgcccgctt
    33001 caccggcgat tttgtcatcc agttcgtcct ggggaatggc ttcatcttct tgtgcagctt
    33061 gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat ttcgttctgt tccagctcaa
    33121 tggttttgac aattggcgtg gcatcgcggt gataaacata gggccgccag acagcgacgg
    33181 ccagagtaag aacggtgagc gaccccaaca taacgcggtg tggtcgcggt aaattattaa
    33241 acgccagggc gacagagcgg gctatctgtt gcacgtaatc acttcctcat taatctcctt
    33301 tcaggcagct cgcatactgg ttggctaatt gactcaggaa ttctgaatag cttgttttac
    33361 ccagtttgat attcgtcccc aggggatcca acgttcccat acgaacggat gtccctcgtg
    33421 cgacgctctc aacgaccgct ggcctgaact gtggctcagc aaaaacgcag gttgcttttt
    33481 gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc gccaggttga atctcagggt
    33541 taacggtaaa atgaccaagc ggtgtcagtc caaactgttt ttcgaaatag ccgtaagcat
    33601 cgtgaaaaac gaaataacct ttccccttga gcggcgcgag ctcgttacca acctgcgttt
    33661 cggttgaggc taattgtgcc tcaaaatcct tcaggttggc gtcaagtttg gctcgacttt
    33721 gcggcataag ttccactaat tttccatgga ttgcaaccgc tgtagcccgc gctatctctg
    33781 gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg atcttcgtca cttttttccg
    33841 cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat cagtagcggt ttcacatctt
    33901 caagctgcgc aatcgttacc tgcttcgctt ctggtaattt gcttaccggt ttttgcataa
    33961 acgcttccat ctccgggcca acccaaacga ctaagtccgc gttctgtaag cgttttacat
    34021 ccgatggacg cagtgaataa tcatgttctg aagcgccgtc aggcagtaaa acctccgttt
    34081 ctgttacacc atcagcaatg gcagaagcga tgaacccaac gggtttaagc gaagcgacaa
    34141 cggcggcatc tgcggcctgt gttgcacctc cccagagagc ggcggataat gctgcgaaaa
    34201 gaagcgtttt tttatgtaac ataatgcgac caatcatcgt aatgaatatg agaagtgtga
    34261 tattataaca tttcatgact actgcaagac taaaattaac atgacaagtc tggtttccct
    34321 ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc tctgatgtgt cgctggaact
    34381 taaacctgga aaaattttga ctttacttgg gccaaatggc gcaggtaagt cgacactggt
    34441 acgggtagtg ctcgggctgg taacacccga tgaaggggtt atcaagcgca acggaaaact
    34501 gcgcatcggc tatgtaccgc agaagctgta tctcgacacc acgttgccac tgaccgtaaa
    34561 ccgtttttta cgcttacgcc ctggtacaca taaagaagat attttgcctg cactgaaacg
    34621 tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag ctctctggtg gcgaaacgca
    34681 gcgtgtacta ttagcgcgag cattgttaaa tcgcccgcaa ttattagtgc tggatgaacc
    34741 cactcaaggc gtggatgtaa atggccaggt ggcgttatat gaccttattg accaactgcg
    34801 tcgcgaactg gattgtggcg ttttaatggt ctctcacgat ctgcatctgg taatggcaaa
    34861 aaccgatgaa gtgctgtgtc tgaatcacca catttgttgt tccggcacac cggaagttgt
    34921 ttccctacat ccggagttta tttctatgtt tggtcctcgt ggtgctgaac aactgggtat
    34981 ctatcgccat catcataatc atcgtcacga tttacaggga cgaattgttt tgcgtcgggg
    35041 aaatgatcgc tcatgattga attattattt cccggttggt tagccgggat catgctcgcc
    35101 tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc gtatgtctta tttcggtgat
    35161 acgctggctc atgcctcatt acttggcgtc gcgtttggtt tgttgctgga cgtgaatcca
    35221 ttctatgcgg taattgccgt tacgctgctc ctggcgggcg gtctggtatg gctggagaag
    35281 cgtccacagc tggcgattga cacgttatta gggattatgg cgcacagtgc cctgtcgctg
    35341 ggcctggtgg tcgttagtct gatgtctaac attcgtgttg atttgatggc ttacctgttc
    35401 ggtgatttgc tggcagtgac gccagaagat ctcatcgcta ttgcgattgg cgtggtcatc
    35461 gtggtggcta ttttgttctg gcaatggcgc aatttgctgt cgatgacgat tagcccggat
    35521 ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat tgttgttgat gctggtgacg
    35581 gcattgacga ttggtgtagc gatgaaattt gttggcgcgt tgattattac ttcactgctg
    35641 attattcctg cagctactgc gcgtcgcttt gcccgtacgc cggaacagat ggctggtgtc
    35701 gctgttttgg tggggatggt ggcagtaact ggcggtttaa ccttttccgc gttttacgat
    35761 acgccggcgg gtccgtcggt ggtgctatgt gcggcactgt tatttattct cagtatgatg
    35821 aaaaagcagg ccagctaatc tatcgctgaa cacatttgtc ggatgcggcg cgagcgcctt
    35881 atccgaccta cggttcgcta tctctggctg gcctgataag acgcgaacag tgtcgcatca
    35941 gacacactgc cagtgtcgga tgcggcgcga gcgaccaatc cgacttacgg catttctggc
    36001 ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca tacgcccacg cggtgtacgc
    36061 tgcaaaaagc cttgctgaat caaataaggt tccagcacat cctcaatggt ttcacgttct
    36121 tcgccaatgg ctgccgcaag gttatccaga cctaccggcc caccaaagaa cttatcgatt
    36181 accgccagca ataacttgcg gtccatataa tcgaaacctt cagcatcgac attcaacata
    36241 tccaacgcct gagcagcgat atctgccgag atggtgccat cgtgcttcac ttcggcgaaa
    36301 tcacgcactc gacgcagcag acggttggca atgcgcggcg taccgcgagc tcgacgagca
    36361 acttccagcg cgccgtcatc actcatctca agccccataa agcgtgcgct gcgactgacg
    36421 atatattgca gatccggcac ctgataaaac tccagacgct gcacaatacc aaaacgatcg
    36481 cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac caatcagggt aaacggcggc
    36541 aaatcaattt taatggagcg tgccgccgga ccttcaccaa tcatgatatc cagttggtag
    36601 tcttccattg ccggatacaa cacctcttcc accactggcg aaagacggtg gatctcatca
    36661 ataaacaaca cgtcatgcgg ttcaaggtta gtgagcatcg ctgccagatc gcccgccttt
    36721 tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca tttcattggc gacgatgttg
    36781 gcaagcgtag ttttacccaa ccccggagga ccgaaaatca acagatgatc gagggcatcg
    36841 ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg aacgaacctg cggctgacca
    36901 acatactctt ccagtaattt agggcgaatg gcgcgatctg ccacatcttc cggcaaagtg
    36961 gtaccggcag aaatcagacg gtctgcttca atcatccttt acctcataac gcggcgcgta
    37021 gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg agcgattttg ctcaccatgc
    37081 ggcttgcctc ttgtggttta tagcccagcg ccaccagcgc ggcaactgct tcttgttcag
    37141 catcgtcggt cgccgggctg gcaggtgatg tgagtaccag gtcggcggct ggcgtaaaga
    37201 gatcgccatg caaaccttta aatcggtctt tcatttcaac aatcaagcgt tcggcggttt
    37261 ttttgccaat acccggcagt ttcaccagcg cccccacttc ttcacgctca acggcattaa
    37321 cgaactgctg cgctgacatt ccggagagga tcgccagcgc caacttcggg ccgacgccgt
    37381 tggttttgat caactctttg aacaatgtgc gctcttgttt attgttaaaa ccgtacagca
    37441 gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat cgcttcctga cccgcttcag
    37501 ggagttcata aaaacaggtc atcggcatat gcacttcata gcctacgccg cccacttcaa
    37561 ttaacaccag cgggggttgt ttttcaatga tgatgcctct gagtctgcct atcacatgac
    37621 gctcctgcgt aatgaatcaa agataatgct gtatgataaa aaaatgctgg atagatatcc
    37681 agcgaaggat gaagaaaact tgcgaggtgt cccgatgatc tgaaaaatgg cagagtataa
    37741 tttattcaac aggttatatt ggaagcaaat atttgaa
//
LOCUS       DAFAVF010000028        37657 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFAVF010000028 DAFAVF010000000
VERSION     DAFAVF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.028"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(239..997)
                     /locus_tag="LGK42_001403"
     CDS             complement(239..997)
                     /locus_tag="LGK42_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK2657666.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1005..2108)
                     /locus_tag="LGK42_001404"
     CDS             complement(1005..2108)
                     /locus_tag="LGK42_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK2657667.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALDYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2118..3299)
                     /locus_tag="LGK42_001405"
     CDS             complement(2118..3299)
                     /locus_tag="LGK42_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK2657668.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3367..4392)
                     /locus_tag="LGK42_001406"
     CDS             complement(3367..4392)
                     /locus_tag="LGK42_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2657669.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPAVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4823..5044)
                     /locus_tag="LGK42_001407"
     CDS             complement(4823..5044)
                     /locus_tag="LGK42_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657670.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5297..8401)
                     /gene="acrF"
                     /locus_tag="LGK42_001408"
     CDS             complement(5297..8401)
                     /gene="acrF"
                     /locus_tag="LGK42_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBK2657671.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8413..9570)
                     /gene="acrE"
                     /locus_tag="LGK42_001409"
     CDS             complement(8413..9570)
                     /gene="acrE"
                     /locus_tag="LGK42_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBK2657672.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9969..10631
                     /gene="acrS"
                     /locus_tag="LGK42_001410"
     CDS             9969..10631
                     /gene="acrS"
                     /locus_tag="LGK42_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBK2657673.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10634..10813)
                     /locus_tag="LGK42_001411"
     CDS             complement(10634..10813)
                     /locus_tag="LGK42_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBK2657674.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(10897..11781)
                     /gene="yhdJ"
                     /locus_tag="LGK42_001412"
     CDS             complement(10897..11781)
                     /gene="yhdJ"
                     /locus_tag="LGK42_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBK2657675.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(11867..12163)
                     /gene="fis"
                     /locus_tag="LGK42_001413"
     CDS             complement(11867..12163)
                     /gene="fis"
                     /locus_tag="LGK42_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBK2657676.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12189..13154)
                     /gene="dusB"
                     /locus_tag="LGK42_001414"
     CDS             complement(12189..13154)
                     /gene="dusB"
                     /locus_tag="LGK42_001414"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK2657677.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13483..14364)
                     /gene="prmA"
                     /locus_tag="LGK42_001415"
     CDS             complement(13483..14364)
                     /gene="prmA"
                     /locus_tag="LGK42_001415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK2657678.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14376..15827)
                     /gene="panF"
                     /locus_tag="LGK42_001416"
     CDS             complement(14376..15827)
                     /gene="panF"
                     /locus_tag="LGK42_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBK2657679.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15817..16059)
                     /locus_tag="LGK42_001417"
     CDS             complement(15817..16059)
                     /locus_tag="LGK42_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBK2657680.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16168..17517)
                     /gene="accC"
                     /locus_tag="LGK42_001418"
     CDS             complement(16168..17517)
                     /gene="accC"
                     /locus_tag="LGK42_001418"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK2657681.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17528..17998)
                     /gene="accB"
                     /locus_tag="LGK42_001419"
                     /gene_synonym="fabE"
     CDS             complement(17528..17998)
                     /gene="accB"
                     /locus_tag="LGK42_001419"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK2657682.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(18976..19950)
                     /locus_tag="LGK42_001420"
     CDS             complement(18976..19950)
                     /locus_tag="LGK42_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2657683.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20102..22042
                     /gene="csrD"
                     /locus_tag="LGK42_001421"
     CDS             20102..22042
                     /gene="csrD"
                     /locus_tag="LGK42_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBK2657684.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22347..23390
                     /gene="mreB"
                     /locus_tag="LGK42_001422"
     CDS             22347..23390
                     /gene="mreB"
                     /locus_tag="LGK42_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBK2657685.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23456..24559
                     /gene="mreC"
                     /locus_tag="LGK42_001423"
     CDS             23456..24559
                     /gene="mreC"
                     /locus_tag="LGK42_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK2657686.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24559..25047
                     /gene="mreD"
                     /locus_tag="LGK42_001424"
     CDS             24559..25047
                     /gene="mreD"
                     /locus_tag="LGK42_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK2657687.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25056..25649
                     /gene="yhdE"
                     /locus_tag="LGK42_001425"
     CDS             25056..25649
                     /gene="yhdE"
                     /locus_tag="LGK42_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBK2657688.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25639..27108
                     /gene="rng"
                     /locus_tag="LGK42_001426"
     CDS             25639..27108
                     /gene="rng"
                     /locus_tag="LGK42_001426"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBK2657689.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27176..30976
                     /gene="yhdP"
                     /locus_tag="LGK42_001427"
     CDS             27176..30976
                     /gene="yhdP"
                     /locus_tag="LGK42_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBK2657690.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWVPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31132..32577
                     /gene="tldD"
                     /locus_tag="LGK42_001428"
     CDS             31132..32577
                     /gene="tldD"
                     /locus_tag="LGK42_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBK2657691.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(32705..33634)
                     /gene="aaeR"
                     /locus_tag="LGK42_001429"
     CDS             complement(32705..33634)
                     /gene="aaeR"
                     /locus_tag="LGK42_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBK2657692.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            33817..34020
                     /locus_tag="LGK42_001430"
     CDS             33817..34020
                     /locus_tag="LGK42_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBK2657693.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34028..34960
                     /gene="aaeA"
                     /locus_tag="LGK42_001431"
     CDS             34028..34960
                     /gene="aaeA"
                     /locus_tag="LGK42_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBK2657694.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            34966..36933
                     /gene="aaeB"
                     /locus_tag="LGK42_001432"
     CDS             34966..36933
                     /gene="aaeB"
                     /locus_tag="LGK42_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBK2657695.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            37025..37297
                     /locus_tag="LGK42_001433"
     CDS             37025..37297
                     /locus_tag="LGK42_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBK2657696.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(37353..37616)
                     /gene="yhcN"
                     /locus_tag="LGK42_001434"
     CDS             complement(37353..37616)
                     /gene="yhcN"
                     /locus_tag="LGK42_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBK2657697.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9292 a         9488 c         9747 g         9130 t
ORIGIN      
        1 tgccagacat caaataaaac aaaaggctca gtcggaagac tgggcctttt gttttatctg
       61 ttgtttgtcg gtgaacactc tcccaaccag gacaaatccg ccgggagcgg atttgaacgt
      121 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccagacatca
      181 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      241 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      301 catcaggtgc agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      361 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      421 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      481 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      541 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      601 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      661 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      721 ccattcccac ttcctgccgg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      781 catctaccac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      841 tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      901 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
      961 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1021 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1081 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggtgggatca
     1141 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1201 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1261 tgtggcagaa taaccagccc ctgagttttc cagtaatcca acgccagcga ctctgccgct
     1321 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1381 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1441 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1501 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1561 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1621 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1681 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1741 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1801 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1861 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1921 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     1981 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2041 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2101 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2161 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2221 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2281 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2341 ggaataatca ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2401 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2461 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2521 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2581 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2641 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2701 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2761 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2821 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2881 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga tagttttcgt
     2941 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcctaaaac agaagcgaac
     3001 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3061 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3121 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3181 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggcg
     3241 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3301 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgccc gttacctcac
     3361 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3421 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3481 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3541 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3601 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3661 ccagcgtaca atcgagaacc attcatcatc gccacgacga actgccggcc caagcggttc
     3721 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3781 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3841 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3901 atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg tagcgccatc
     3961 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4021 ataagtgacg ccggtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4081 attacgggag agcaaatcca cttcccctga ctgtaaagcg gtgaagcgtt cttttgctgt
     4141 gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4201 aacatcaaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4261 tccatcactg atcccgcatt gcacaaaacc ttttttctga acggcatcaa gcgtcgcgcc
     4321 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4381 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4441 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccagc ttttgcgccc
     4501 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4561 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4621 gcagaataat gattgttagc gtgagtatat ttctggtggg aagtgtgaaa gttaaattga
     4681 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaatcag cagagaatta
     4741 gccagcagaa tatagcaata ttttatctta tgtaaagata ctgtatcgtt aaacagagcg
     4801 cgaaactccg cgccctgttc agttatttcg acagagcgat gatccccatc gtcaggccag
     4861 caacagccgc tgccccgcca gcaatagccc cagccgtttc ccagttatcc tgagtcgtgc
     4921 ctttcataca ggtattggtg tgaaccagac gaccctgatc gtcataaacc ggaacgcagg
     4981 gcgaatcgtg agcacagccc gccagcaatg cggtgagcaa tgccacagga attaatcttt
     5041 tcatggtgtt acctcagtaa taaacgctaa cgcttaataa gttaactctc ttattaacga
     5101 gctgaatgcc attttatcac cggaggttat aaatcttgcg gaatgaataa tgacaaatta
     5161 tgtgaattgt aaaaatagtg aagggagcgg gaactataga aagcttttaa agagtgagca
     5221 gaagaatatc ttattaattt ttatgggttt cactggaaat aataaaggca cccgaaagcg
     5281 cctttatgtt tctgatttat cctttaaagc aacggcggat caccacaaag aacaccggta
     5341 cgaagaagat tgccagcaac gttgcagaga ccattcctcc cattaccccg atacccacag
     5401 cgttctgcgc gccactgccg gcaccgttac tgatagctag cggtaatacg ccgagaataa
     5461 aggcgagaga ggtcatcagg ataggacgca gacgcatacg tactgccatc agtgtcgctt
     5521 caacaacacc tttaccctct ttctccatga gatctttagc gaactcaaca atcaaaatag
     5581 cgtttttggc cgacaagcca attgtcgtta gcaagcccac cataaagtag acgtcatttt
     5641 tttgattaaa gagtgttgcc gccagtagca cgccgacaat ccctaacggc actaccaaca
     5701 taaccgagac aggaattgac cagctttcat agagtgcagc aaggcacagg aaaacaacca
     5761 caaaggaaat tgctaccaga gcgggagcct ggtttcccga taagcgttcc tgatacgaca
     5821 tacccgtcca gtcataacca atgcccgcag gtaattttga cgcaaggttt tccatcaacg
     5881 ccatggcatc gccggaactg gttcctggcg cggcttcccc ctgaatctcc attgacggca
     5941 gaccgttgta gcgttccagt cgcggagagc catacaccca atgtgaagtg gtaaaggccg
     6001 agaatggcac catttcgccg ttggcgctgc ggacataaag tttatcgaca tcttctggca
     6061 gcatacggaa tttggcatcc gcctgaacat acaccttttt cacgcgacca cggtcgatga
     6121 agtcgttaac gtaagtccca cccaacgccg ttgaaatggt ctgattgatg tcagaaagtg
     6181 aaacacctaa tgcctgcgcc ttttcctggt caacttccag tttaaactgc gcggtgtctt
     6241 ccaggccatt aggacgcacg ctgactaagc tggcaggatg ttgcgccgcc atgccaagca
     6301 actggttacg ggcctgggtc agggcatcgt gacccagccc agcctgatca attaactcaa
     6361 agtcgaaacc cgttgccgtg cccagttcaa caatggctgg catattgaat ggaatgacaa
     6421 aaccgtcgcg gatcttgccc aattccattt tggcacgatg gattaccgct tccgcactgt
     6481 tttcatcact attacgctct tcccacggtt tcagactgac gaaggccata ccggcgtttt
     6541 gtgcctggcc gctgaagcta aagccgttaa ccgtaaagac actttcaacg ttcgctttct
     6601 cgttcttcag atagtaatcc gtcacttgat ccaacacttt ttgcgtccgc tcttgcgtcg
     6661 cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca ggtaagaagg
     6721 aagacggaag acgtaaaaac aacaccacca ttcctgcaac aatcagcgca tagatcagta
     6781 aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt ggtgtagtgg ttaacgctat
     6841 gatcgaaggt ggtattaaac cagccgaaga aaccaccctt attttcgtga tgctcagctg
     6901 aggtgggttt gagcagtgtt gcacataacg caggggtaag aatcaatgcc accagaacag
     6961 aaagcgccat tgccgaaacg atggtgatag agaactggcg ataaattgcc ccagtagaac
     7021 cgccgaagaa tgccatcgga ataaataccg ctgacagcac catcgcgata cccaccagtg
     7081 cgccctgaat ttgcgacatc gatttttccg tcgcttcttt tggcgggagc ttatcctcca
     7141 tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc atcgacgagc agcccgatgg
     7201 caagcaccat cccgaacatc gttagtgtgt tgatggagta accaaaagcg gcgaggatgg
     7261 caaacgtccc taataacacc acgggtaccg caatggtggg gatcagcgtt gctcgcatat
     7321 tctgcaagaa cagatacatc accaggaaca ccagcataat ggcttcgaac agcgttttta
     7381 ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc ataagggtag agaaccttca
     7441 ttccctgcgg gaagaatggc tgtaattccg ccagttttgc cttaatggct ttcgcggtat
     7501 cgagagcatt cgcgccggtt gccagcttaa tccccaggcc cgccgccggt tttccgttga
     7561 tacgagcgat aacgttatag ttttcaccgc caagttcaac tcgtgcgaca tctttcaggc
     7621 gtaccaccga gccgtcactg tttacgcgca gggttacttt gccgaattct tccggatttt
     7681 ttaaccgcgt ctgagcaata attgaggcgt tcaactgttg ccctggtaac gctggcgttc
     7741 cgcccaactg tccggcagcg atctgatcgt tctgtacctt caactggtta atcacatcaa
     7801 ccggtgtcag tttatatttg tttagcagat cggcgtccag ccagatacgc atcgcatact
     7861 gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg gctaagcgta tctttaacgt
     7921 tagaggccac atagtccgag atatcgtcct gtgtggtgtc tgggttatca gagacaaagc
     7981 ccgccaccat caaatagctg ctactggact tttcaacact gatcccctgc tgctgaacct
     8041 cctgcggcag caacggcgtg gcgagctgga gtttgttctg cacctgcact tgcgcgatat
     8101 caggatcggt cccggactgg aaggtaaggg taattgtcac gctaccggcg gaatcgctgg
     8161 tggaggacat atacatcagg ttatcgatac cgttcatatt ctgttcgata acctgcgtca
     8221 ccgtatcctg cacggtctgc gcatcagcgc ccggatagtt tgctgaaaca gaaaccgcag
     8281 gcggtgcaat ggttggatac tgagcgacgg gtaattgtag gatcgccagt gcgcccgcca
     8341 tcatcagaat aatggccagc acccatgcaa atatcggtcg tcgaataaaa aagtttgcca
     8401 tgtcagatta ccttacttcg atgcagtatc tgcgggggta tcggtagtgg ctttaacctg
     8461 ctctcccgga cgcgcttttt gcaggccgct gacaatgact tgatcgccag atttcagacc
     8521 ttcgctaatc aaccatttat cgccaatcgc ctgactggca acgacagggc gcgcttccac
     8581 ctgactttta tcgttaacaa tcagcacggt tgcatcacca cgcggtgtgc ggctaacgcc
     8641 ttgttgcggg ataagaatgg cgtcaggttg gacgccttca tcaatccgtg cgcgcacaaa
     8701 catacccggc aaaagcgtat gttgcgggtt agggaagacg gcacgtaggg ttatggagcc
     8761 ggtgctttca tcaacggtca catcagagaa ttgcagcgta cctttcaggg gataggtttg
     8821 accgttttcc atgaccaact ctacgttgct ggtggcgttt tccttatgca aatttccttg
     8881 ctctacggat tgcttcagcc tcataaaatc gttgctggat tgggtcacat caacgtagat
     8941 aggatcgagc tgctggacag tcgccagttc agtcgtttgc ccattagtga caagagcgcc
     9001 ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc gcagtcactt tggtataagc
     9061 gagattgatg cgagcgcttt cgactgtggc ttttgcggca atcacggcgg catcggcctg
     9121 gcgagcatca gcaatggcct ggtcgtactc ctgctggctg atgtatttcg tacccacgag
     9181 cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg gcggcttcac ttttcgccag
     9241 ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg ggatcgatct ggtacaggga
     9301 ctggcctgct tgtacatcgc tgccttcagt gaaattacga ttcagtacga tcccgctaac
     9361 ctgtgggcga acttcggcta tacggtaagc attggtgcgg cctggtaatt cagtcttaac
     9421 ttctaacggg gccgttttca caatatgaac ggtaacctgc ggttcaccga cgtgagcttt
     9481 ctcttctccc ttatcgttac aaccggcgat taaagccgcg gagatcagaa taaaggaggg
     9541 caggaggaaa aacctggcat gtttcgtcat tactattcct caaaaaacca aaagcgcgtt
     9601 atttacccag aaaggcaacg cgttaactcg cagaaagaaa aatacagttt gctatcctac
     9661 aaattatcat tcgtcgatgt aaggaatagt tatgaatact ggcgtctcaa ggcacataaa
     9721 cacaaaaaaa gattaatatt ctactgtttt attttgacgc gggttgaaag aggcagaatt
     9781 aaaacctcgt aaattgaaat atatattcat gtggcgaatg tattttaggt aaataatata
     9841 tattatttat ttgacgattt atagcaactc aatattagcc tctcgtataa atacacatta
     9901 ggtgatagat taaccttcgc tattttctca ctctgtgtcg aatatattta tttcctgaat
     9961 aattaatcat ggcaaaaaga accaaagccg aagctctgaa gacccggcaa gaactgattg
    10021 aaactgccat cgcccagttt gcgcagcatg gcgtaagcaa gacgacactc aacgacattg
    10081 ccgacgctgc taacgttacg cgtggcgcta tctactggca cttcgaaaac aagactcaac
    10141 tgtttaatga gatgtggttg caacagcctt cattgcggga gttaatccag gaacacttga
    10201 cggctggatt agagcatgac ccgtttcaac aattgcgtga aaaattgatt gtcggcttgc
    10261 aatatattgc caaaattccc cgccagcagg cgttgctgaa aatcttatat cacaaatgtg
    10321 aatttaatga tgagatgctg gccgagggag tgatacgcga aaagatgggc ttcaatccgc
    10381 agactctccg cgaagtattg caggcgtgtc agcaacaagg ttgtgtagca aataacctcg
    10441 atttagatgt tgtgatgatt attattaatg gtgccttcag cggaattgtt caaaactggt
    10501 taatgaatat ggcgggttat gatctttata aacaagcccc cgctctggtc gataacgtat
    10561 taagaatgtt catgccagat gaaaacataa cgaaattaat tcatcaaacg aatgaattaa
    10621 gtgtcatgta aattcacatt ctgtcctgaa aattcagttc gtcacagtta accagtttta
    10681 gtggattaac tgagttcata ttgcggcatt tgtctgtttc tgttgccagc agttcaatcc
    10741 actgcatcag taaagtatca aacagaaaaa cgaagacagc gaaaacgacg agccaccaat
    10801 acttgcgaat cattacctga gtctgtgtat gtttaaccaa gtctggcagg aaatatacac
    10861 gaaatgaata atctgaaaac caggcttaca cttcctttac tttgcaatga gatcggggtc
    10921 aacttcgctt aaccggcttc gttttgacag gttgccggtc tttctttttt tcactttcgc
    10981 cagttcttcc gcagagtaat gcgacgcgac atccagccgt cgaagcccca ttttgatgta
    11041 ctcgctgttg atctcaatac caatgaattt tcgcccgctg gagacggcta cggcaccggt
    11101 agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct gggttggaag aggcgagaat
    11161 aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg ttctcatatt catccatcaa
    11221 ataacgcacg cgcggaaaat cccagacgtt acccggtact ttttgatgat tgtatggctg
    11281 tggaggattt ttgcgataat cgatcaacgc gcgctgcgat ccggttttgg cttctaccag
    11341 aatagcatca ccgttgaatg tgtagttctt tgcgtctttc accatcatca ggatgggttc
    11401 gtacatggag ccgtagtgtt ttttcgcctg tactccagaa ctgtcatatg accagacgat
    11461 gcgacttttg atggtaaaaa gcttgcggca ctggagatcg ataaagggca tgttttccgt
    11521 gctgttcatg atgtacatgc tgccctgctt tttcagaacg cggtggcact ctgcaatcac
    11581 ttcaaacagc cagtcgataa acagatcttc tttccaggct tcgatcagac catcaaaatt
    11641 tttaccgatg ttatatggtg ggtcggcaaa gatcagatcg acactttcgg cggggatctt
    11701 tttaagttcg gcaagcgcat caccgtgaat aatggtctta gcttcattac caaaccgggt
    11761 cggttcacat cctgttctca tggtcactcc ctttgtgaca cctataaaaa aggcgcttcc
    11821 ccatgccgag tagcgccttt ttaatcaagc atttagctaa cctgaattag ttcatgccgt
    11881 attttttcaa ttttttacgc agcgtaccac ggttgatgcc catcatcagc gcagcacggg
    11941 tctggttacc acgggtgtat tgcatcacca tgtccaacag gggctgttct acttcagcca
    12001 gtaccagctc atagaggtca ttcacatcct gaccattcag ttgagcaaaa tagttcttca
    12061 gtgcctgttt aaccgagtca cgcaggggtt tttgggttac ctgatcctga gagttaacgg
    12121 tagaaacggt cagtacgtca gaatttacgc gttgttcgaa catagttctg tcagctcttt
    12181 atttctgttt acgcaaaatt ttcgaagtat gcctccaacg cctccagctg ttcgctggca
    12241 tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg gagcgtgttc ctggagatac
    12301 caggaaacgt gtttacgtgc aattcggtac ccttttgccg gaccataaaa gtcatgcagt
    12361 tcccgaacgt gcgcgcaaag caagcgctta acctctgcca aaggcagcgg gggcagcaac
    12421 tccccagtgt ccagataatg ctggatttcc cgaaagatcc agggtcttcc ctgagctgcg
    12481 cggcctatca tcagggcatc cgcccctgta tagtcgagca cagctctggc tttaagcggg
    12541 tcagtaatgt cgccattcgc gataaccgga atggaaactt tctgcttaac tgcccgaata
    12601 ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg tacggccatg aatggtcaga
    12661 gcctgaatgc cacagtcttc agccagttgg gcaatctctt cgcagttacg gtgttccggt
    12721 gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca ctgcattgac gacctcggta
    12781 aggatcgatt taacgacatc cgggtactgc aagagggctg aacctgcgag cttgcgattc
    12841 acttttttag ccgggcaacc catattgata tcaataatct gggcaccgct ttccacgtta
    12901 atacgtgctg catctgccat ttctttcgga tcgctaccag caatttgcac ggtgcgaata
    12961 ccgggttcat caatgtgcac catccgtaaa cgagatttgt cgctttccca aacctgtggg
    13021 ttagaagaca tcatctcgga tactgtcaat ccggctccca tctcgtagca caacgtccga
    13081 aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca ggcgatttct gagctgatat
    13141 tgtccgatgc gcatgagtta agaaatgacc atactgtgac tgcaaggcgg cgtatattac
    13201 gcattttttg cacgagatga aaggccaaac tttgaccaat cctctgagat ggatcaaaga
    13261 attgcattta aaatgagcgt ggtgcgataa ttactcataa aaatcatcat attagaaaat
    13321 agtgactaaa atttacactc aaagaaattt gagtaagttc tcaatttttc tttatgaatg
    13381 aaaattttgg cacgcaaatt tgcgtaaata accggcaaat ttacgtgcct tttgtgagct
    13441 tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa ggttaattct tacgaccggt
    13501 aatacggcac cactcttctt tttccacgac cgggtccagt gcgaagctat cggcataagc
    13561 ttcacaaacg ctctctgcct ggcttgccag aataccggaa aggcccagca aaccgcctga
    13621 aaccggcagg acgctgatta acggtgccag ttcacgtaat gggcctgcaa ggatgttagc
    13681 gaccaccacg tcggctttca tttcttctgg ctgatctttc ggtaagtaga gttccagacg
    13741 gtcagaaacg ccattacgtt cggcgttgtc gcggctggcc tgaatcgcct gcggatcgat
    13801 atcaatacca atggcttttg ctgcacccag tttcagcgcc gcgatcgcca gaatgccgga
    13861 accacagcca aagtcgatga ctgttttacc ggttaaatcg aggctgtcga gccattgcag
    13921 gcacagagag gtggttggat gggtacccgt accaaacgcc agccctggat ctaacatcac
    13981 gttgacggcg ttttcgtccg gcacatcacg ccagctaggg cagatccaca gtcgttcacc
    14041 aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc cagtctttat cttctagttg
    14101 ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg ttttccagaa tcgccaccac
    14161 gtcgttcata tcggtttcag cgtcgaacag accaatcaca tcggtgtcgc cccacaggcg
    14221 cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg gtatcctgaa aagtgataga
    14281 aacggcaccc gcttccatca gcgcatcgct aagatcttcc gcgttcgcgc cggtggtgtt
    14341 cagtttcagt tggatccaag gcatggcaaa actctttatt tatcagtagt caaaacggta
    14401 gcttgcggga cggatgtacc gaaacggttt ccgaccagga aagccagcaa acttagtagt
    14461 aacgagggca caatagggtg gaagcccagg tactgaatat tcagcgtcgc gagtacggca
    14521 tacagcacgc cgccaacgat catcgcactt agcgcgcctt tggcgttggc gcgttcccag
    14581 taaagaccca gcaccagcgg ccacaggaaa acggcttcca gcccaccgaa ggccagcaaa
    14641 ttcagccaga tgatcatttc tggcggcttc caggcggcaa gcagcagcaa cgcgccgaga
    14701 actaacgtaa ttaccgccga catccgcttc agacgcgtct cgttttgcat ttgatccgga
    14761 cggatattca gatagagatc tttaatgatc gtagcggaac tttgcagcaa ctgggcattg
    14821 atcgtcgaca tgatcgcagc cataggtgca gccaggaaga ttccggcagc aaatggtggc
    14881 agcactttta ccattaacgt cgggatcacc agatccggta cggtgagatc ggggatcact
    14941 gcacgaccta acgctccggc caggtgcata ccgaacatca ggattgcgac tacaatcgta
    15001 ccgatgatga tcccccggtg tactgctttg ctgtctttat aagagataca gcgcaccgca
    15061 gtatgcggca ggccaatcac gccaaagcac accagtaccc agaacgacgt cataaaggca
    15121 ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt gcggatcgat ggtttgcaag
    15181 gtctgtactg cgttgcttaa gccgccagcg gcatgtacca cgccaataag cagcacaacg
    15241 gtgccaatca gcatcacaag cccttgcatg gtgtcgttca gcacgctggc gcgaaagcca
    15301 ccaaaggcgg tatataacgc aatgctgata ccaaaaatca gcagcccggt ttcataagga
    15361 atacccgccg cggtttccag caggcgcgca ccgccgataa actgcacggt catcgcacca
    15421 acgaacgcaa ccagcaaact caaactcgcc agccacacca gaagacgact ctggtagcgg
    15481 gcaaacagca tatcgttcag cgtcactgca ttgtagcggc gcgcaagaat cgcaaacttc
    15541 ttgccgagaa taccgagtga aagccagact gcaggaagct gaatcatcgc cagcaatacc
    15601 cagcccagcc cgtatttata agcagctcct ggcccgccga taaacgaact ggcactgata
    15661 taggtcgcgg tgagcgtcat cgccagcaca ataccgccca tagagcggct gccgaggaaa
    15721 tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg cataaacaga gataccgaac
    15781 accaccacca gataggcgac cagcggtaga attacttcaa gctgcatcgt catcctccag
    15841 tgggatatcg cgatagataa atttcaccat cgcccagcac agtccaataa acagtagcgg
    15901 cgtcaggatg caggccatct caaaccagcg cggaaagccg gtaaaaccgg gggcaacgcc
    15961 aggtaagtaa gcggctacta accaaactgc cagatacaaa agggtcagcc ccagcgccca
    16021 gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt ttttgtccct gatgggtgaa
    16081 gaaagcgagg attgtacctt atgggggttg tcgatcccca gtaataaaaa aggccggaaa
    16141 atccggcctt ttgacgcttt tgcagtctta tttttcctga agaccgagtt ttttctccag
    16201 atagtggatg ttagtgccac catgctggaa gttctcgtca ttcatgatgc ggatctgcag
    16261 atcaacgttg gttttgatac cgtcgatgat cagctcctgc agcgcattct tcatgcgagc
    16321 aatcgccacg tcacggtttt caccgtagca aatcagctta ccgatcattg agtcatagta
    16381 cggcggtacg gtgtagcccg cgtagatatg agactcccaa cgcactccaa aaccgccagg
    16441 tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag gtgttcggat cttcggcgtt
    16501 gatacgacat tccaccgcat ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg
    16561 ttgaccggca gcgatacgca gctgttcttt gatcaggtca acgccggtga tcatttctgt
    16621 aaccgggtgt tctacctgaa tacgggtgtt catttcgatg aaatagaact cgccgttttc
    16681 gaacaggaac tcgaaagtac ctgcaccgcg atagccgata tcaacacacg ctttagcaca
    16741 acgttcgccg atgtagcgac gcagttccgg tgtaattccc ggtgctggtg cttcttcgac
    16801 cactttctgg tgacgacgct gcatagagca gtcacgttcc gccagataga tagcgttgcc
    16861 ctgaccgtca gccagtacct gaatctcgac gtggcgagga ttttccaggt atttctccat
    16921 gtaaaccata tcgttattga atgcggcttt cgcttccgca cgggtcatgg agatggattg
    16981 tgccagttca gcgtcgccgc gcactacacg cataccacga ccgccgccgc cgccggaggc
    17041 tttgataatc accggataac ctatgcgttt agcaatggca cggtttttat ccatatcgtc
    17101 gcccagcggg ccgtcagaac ctggtacgca aggaacgccc gcttttttca tcgccgcgat
    17161 tgccgacact ttgtcaccca tcaggcgaat agtgtctgct ttcgggccaa tgaagataaa
    17221 gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg gagaggaagc cgtaacccgg
    17281 atggattgct actgcgccgg tgatttcagc ggcgcttatg attgccggga tgttcagata
    17341 actttttact gacggagcag ggccaataca gaccgtttca tctgccagta atacgtgttt
    17401 tagatcgcga tccgcgctgg agtgcacagc gacagtcttg atgcccagtt ctttacaggc
    17461 acgaagaata cgcaatgcaa tctcgccgcg gttggcaata acaattttat ccagcatgtt
    17521 cgcctcgtta ctcgatgacg accagcggct cgtcaaattc taccggttgt ccactttcga
    17581 ccagaattgc tttcacggta ccggatttgt ccgcttcgat ctggttcatc attttcatgg
    17641 cttcaacgat gcacagggta tcgcccacgt tgactttctg acccacttcg atgaacgctt
    17701 ttgcgtccgg gcttggggtg cggtagaaag taccaaccat cggggagcgt acgatgtgac
    17761 cactgatttc cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt
    17821 tagattgagc tggctgctgc atcattggtg cagcgtaagc ttgttgcatc acagggaaac
    17881 ttgcggcagg agctgcacgg ctgatgcgta ctgactcttc gccttcggag atttccagtt
    17941 cggagatgcc tgattcttca accagctcga tcagtttttt aatcttacga atatccatga
    18001 gtgggttccg tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa
    18061 gcgtatgccc cgacagctgt atgcatagcg ataaattcca gcaggccggg acgccggtct
    18121 attttgcccc agagtgtcaa tgttagactt gacggacatt gtgcagcgat ctgctgctat
    18181 cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa
    18241 aaactgtgtc agggaggacg aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta
    18301 aatactggtc ttatcaggga agataatcaa cagctaacat gtaaataacc ttcaacaccg
    18361 tgtaatttgc aacaagccgc acaattcacg aaattagcgc catcatcgac ggaacttctt
    18421 ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag atgaggggct gcaatgagat
    18481 aatcttcaca gaccacaaat aatgtaggga tgccaggatc gcgacaagat agacgacatt
    18541 acgcagcggt tgctggggat tacccaattt tcactgcatc gtctggatag atgtgaatgc
    18601 taaagcacgc aaaatcaacc gactgacaat tctcagcatt aaataggttc gagtggttaa
    18661 tttgtagtca aataaaatca gattattcac cccatgggac cagaaatcag taataggtta
    18721 agcgtagtgt cgcccaggca aagcaccata aacctaacag gcgacggagg catatcaata
    18781 atggctgttt agaggagtat caatgcgcat caatttgtgg tcagtcgaaa tcatgacttt
    18841 ccagtttgga tgatgttgta caacttcctg accaaggtgt tcgacgaaag ctgtccggct
    18901 aaccgctttt cccgttaacc gattgaataa tgatgtcaaa atgagttccc tctcttatta
    18961 ttccttgcta aatggttagt taaccttcac cagcgtgcga ccctggatct ggttattaat
    19021 gatggcctcg gcaaagttcg gtgcctctga cagagatatc tcttttgccg cctgggtata
    19081 gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct tgtgcgcggc gttctggtgg
    19141 cgtcattact gaatccaccc cttgcaaacg gacattacgc agaataaatg gcatgaccgt
    19201 ggttggcaga gtaaaaccac ccgccagacc acaggccgcc acgcagccgc cgtaattcat
    19261 ttgcgccagc acttttgcca gcactttgtc gccaacagtg tcaattgccc cagcccagac
    19321 ttgtttttcc agaggacggg attcggcaaa ctcatcacga gggagaatac gactggcacc
    19381 taaacttttc aggtattcat gggtactttc gcgaccggaa acggcaacga cctgataacc
    19441 caacttatgc agcagcgcca cggcggtact gccgacgcca ccactggcac ccgtcacgac
    19501 aatctccccg tcctgcgggc gaacaccggc atcttccagc gccatcacac acagcatggc
    19561 ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg tccagccctt gcggcatggc
    19621 aaccagccag tcacctttca ctcgcgcctg ctccgccagc ccaccccagt ggttttcacc
    19681 aacgccccag ccagtgagta acacctcctg accggcatga aaacgcggat cttcgctggt
    19741 gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga aaattacgga tgatttttcc
    19801 cttaccggta atcgccagcg catctttata gttcaggctc gaccagtgaa catcaaccgt
    19861 gacatcgccc tccggcaggc gactttcgtc aagtgtctgt accgatgcga gagttttgcc
    19921 gtcctgctgt tctaaaagta acgcctgcat aagtggtcct catgtgcatg atggattgga
    19981 aaataatttc tgaagactat actcgctaat ggaaaagcaa agccgatgaa gcgcaataaa
    20041 ttgcgagata aatctgattt gctagtatgc ccgcttcctc actatcggag ttaacacaag
    20101 gatgagatta acgacgaaat tttcggcctt tgttacgctg ctcaccgggt taacaatttt
    20161 tgtgactttg ctgggctgtt cgctaagttt ctacaacgca attcagtata agtttagtca
    20221 tcgcgttcag gcggtggcga cggcgattga tacccacctt gtgtcgaatg acttcagcac
    20281 attaaggcca caaattaccg aattaatgat gtcggcagat atcgttcgtg tagacctgct
    20341 ccatggtgat aaacaggttt ataccctggc cagaaatggt agttatcgtc cggttggctc
    20401 cagcgatctg tttcgcgaac tgagtgttcc gttgataaag catccgggga tgtcgctgcg
    20461 tctggtttat caggatccga tgggcaacta tttccattct ttaatgacta ccgcgccgct
    20521 cacgggggcg attggcttta tcattgttat gctcttcctg gcggtacgct ggttacaacg
    20581 gcaacttgcc gggcaagaat tgctggaaac ccgggctact cgtatcttaa acggtgagcg
    20641 tggctctaat gtgttgggaa ccatctatga atggccgccc agaaccagca gtgcgctaga
    20701 tacgctgctt cgtgaaattc agaacgcacg cgaacaacac agccgtcttg atacgctgat
    20761 ccgctcttat gccgcccagg acgtgaaaac cggcctcaat aaccgactct ttttcgataa
    20821 tcagttagca acgttactgg aagatcagga gaaagtaggt acccacggga tcgtgatgat
    20881 gattcgtctg ccggatttca atatgttgag cgatacctgg gggcacagcc aggttgaaga
    20941 acagttcttc actctgacga atctgctgtc gacatttatg atgcgctacc ctggcgcact
    21001 gctggcgcgt taccaccgca gtgattttgc tgcgctgtta ccgcaccgga cgttaaaaga
    21061 ggcagagagc atcgccggtc agttaatcaa agccgtcgat accttgccga acaataaaat
    21121 gctcgatcgc gacgatatga tccacattgg tatctgcgcc tggcgtagtg gtcaggatac
    21181 cgagcaggta atggaacatg cagagtctgc cacgcgtaat gcgggattgc agggcggcaa
    21241 tagctgggct atttacgatg actcgttgcc tgaaaaagga cgcggtaatg ttcgctggcg
    21301 tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc ctttatcaaa aaccggcggt
    21361 tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc cgcatcttcg atggtaatga
    21421 agaggttagc tcggcggagt atatgccgat ggtcttgcag tttggcttat cggaagagta
    21481 tgaccgtctg caaatcagcc gtcttattcc actattgcgt tactggccag aggaaaatct
    21541 ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt tttcagcgtt ggctgcgcga
    21601 tacgttaatg caatgtgaaa aatcacaacg aaaacgcata attattgaac ttgcagaggc
    21661 cgatgtaggt caacatatca gtcgtttaca acctgttatt cgtttagtga atgctttagg
    21721 ggtacgggta gccgtcaacc aggctggttt gacgctggta agcaccagtt ggatcaaaga
    21781 acttaatgtt gagttactca agctccatcc ggggctggtc agaaacattg agaagcgaac
    21841 ggagaaccag ctgctggttc aaagcctggt ggaagcctgc tccgggacca gcacccaagt
    21901 ttacgccacc ggtgtgcgtt cgcgaagcga gtggcagacc ctgattcagc gcggtgttac
    21961 aggcgggcaa ggggattttt tcgcgtcctc acagccactt gatactaacg tgaaaaaata
    22021 ttcacaaaga tactcggttt aacctgccgt ttaatctgtt ttcacgtaga ataacgcgcg
    22081 ctgcgtctca tgggagtgtg cttgtctgct cgccagattg ttgcagcaca tatgcagatg
    22141 aatgacctta cgcggttgca aacaggcgag gaatgctgct gatgcattaa gcctttctgg
    22201 actcaggcag agatttgtaa caaaggaaac gaactgcact aattttcacc gtagcagatg
    22261 atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt aagcggattt tcttttccgc
    22321 cccagctttc aggattatcc cttagtatgt tgaaaaaatt tcgtggcatg ttttccaatg
    22381 acttgtccat tgacctgggt actgcgaata ccctcattta tgtaaaagga caaggcatcg
    22441 tattgaatga gccttccgtg gtggccattc gtcaggatcg tgccggttca ccgaaaagcg
    22501 tagctgcagt aggtcatgac gcgaagcaga tgctgggccg tacgccgggc aatattgctg
    22561 ccattcgccc aatgaaagac ggcgttatcg ccgacttctt cgtgactgaa aaaatgctcc
    22621 agcacttcat caaacaagtg cacagcaaca gctttatgcg tccaagcccg cgcgttctgg
    22681 tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc aattcgtgaa tccgcgcagg
    22741 gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat ggctgccgca attggtgctg
    22801 gcctgccggt ttctgaagcg accggttcta tggtggttga tatcggtggt ggtaccactg
    22861 aagttgctgt tatctccttg aacggcgtgg tttactcctc ttccgtgcgc attggtggtg
    22921 atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa ttacggttct ctgatcggtg
    22981 aagccaccgc agaacgtatc aagcacgaaa tcggttcggc ttacccgggc gatgaagtcc
    23041 gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt tccacgcggt tttaccctga
    23101 actccaacga aatcctcgaa gcactgcagg aaccgctgac cggtattgtg agcgcggtaa
    23161 tggttgcact ggaacagtgc ccgccggaac tggcttccga catctccgag cgcggcatgg
    23221 tgctcaccgg tggtggcgca ctgctgcgta accttgaccg tttgttaatg gaagaaaccg
    23281 gcattccagt cgttgttgct gaagacccgc tgacctgtgt ggcgcgcggt ggcggcaaag
    23341 cgctggaaat gatcgacatg cacggcggcg acctgttcag cgaagagtaa tcggatgcag
    23401 gcaggggaaa tgtctgttta ccctgcctgg tctgatacga gaatacgcat aacttatgaa
    23461 gccaattttt agccgtggcc cgtcgctaca gattcgcctt attctggcgg tgctggtggc
    23521 gctcggcatt attattgccg acagccgcct ggggacgttc agtcaaatcc gtacttatat
    23581 ggataccgcc gtcagtcctt tctactttgt ttccaatgct cctcgtgaat tgctggatgg
    23641 cgtatcgcag acgctggcct cgcgtgacca actagaactt gaaaaccggg cgttacgtca
    23701 ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga caatacaaac aggagaacgc
    23761 gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat gagcagaaaa tggtgactca
    23821 ggttatctcc acggttaacg atccttatag cgatcaagtt gttatcgata aaggtagcgt
    23881 taatggcgtt tatgaaggcc agccggtcat cagcgacaaa ggtgttgttg gtcaggtggt
    23941 ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt gatgcgaccc acgcgctgcc
    24001 aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc ggtaacggtt gtacggatga
    24061 tttgcagctt gagcatctgc cagcgaatac ggatattcgt gttggtgatg tgctggtgac
    24121 ctccggtctg ggtggtcgtt tcccggaagg ctatccggtc gcggttgtct cttccgtaaa
    24181 actcgatacc cagcgcgctt atactgtgat tcaggcgcgt ccgactgcag gactgcaacg
    24241 tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac ggcgctaacc cgatgacgcc
    24301 ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag atgatgccgc aggtattgcc
    24361 ttcgccagac gcgatggggc caaagttacc tgaaccggca acggggatcg ctcagccgac
    24421 tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct gctgcgccca cacagcctgc
    24481 tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt ggtgctcaac cgcctgcgcg
    24541 tgcgccggga gggcaatagt ggcgagctat cgtagccagg gacgttgggt aatctggctc
    24601 tctttcctca ttgcgctgtt gctgcaaatc atgccctggc cggataacct gattgttttc
    24661 cggccaaact gggtgttact catcttgttg tattggatcc tggccttgcc tcatcgcgta
    24721 aatgtgggca caggttttgt gatgggtgcc atactggatc tgatcagcgg ctcgacgctt
    24781 ggcgtacgcg tattggcgat gagcatcatt gcttacctgg tggcgctgaa ataccagctt
    24841 ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca tgttgctttc gctggtggtg
    24901 gatattattg ttttctgggc agagttttta gtgattaacg tctctttcag accggaagtg
    24961 ttctggagta gtgtagtcaa tggggtgctc tggccgtgga ttttcttgct gatgcgtaaa
    25021 gtccgtcagc agtttgcagt gcaataaagg tttctatgac ttctctgtat ttagcttccg
    25081 gttctccgcg tcgtcaggag ttacttgcgc aacttggcgt gacctttgaa cgtattgtta
    25141 caggcattga ggagcagcgt cagccgcagg agagcgcgca gcagtatgtt gtgcgtctgg
    25201 cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca ggatctcccg gtgctgggtg
    25261 cggatactat cgttatcctg aatggagaag tgctggagaa accgcgcgac gcagagcatg
    25321 cggcgcagat gttgcgcaaa ttatcgggtc agacccatca ggtgatgacg gcagtggcgt
    25381 tggccgacag ccagcacatt ctcgattgcc tggtggtcac cgatgtgact ttcagaacgt
    25441 taacagacga agacatcgcg ggctatgtcg ccagcggtga accgttagat aaagcaggtg
    25501 catacggtat tcaggggctg ggtggctgtt ttgtcaggaa gataaatggc agctatcacg
    25561 ccgtagtcgg cttaccgctg gttgaaacgt atgaattatt aagtaatttt aacgcactgc
    25621 gtgagaaaag ggataaacat gacggctgaa ttgttagtaa acgtaacgcc ttcggaaacg
    25681 cgagtggcgt atattgatgg cggtattctg caggaaattc atattgaacg tgaggcgcga
    25741 cgcggaatag taggcaatat ctacaagggt cgtgtaagtc gtgtacttcc gggtatgcag
    25801 gcggcttttg tagatattgg gctggataaa gccgcgtttc ttcatgcatc cgatatcatg
    25861 ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat tcacggtgcg cgacatctcg
    25921 gaactggttc gtcaggggca agatctgatg gtgcaggtgg tgaaagatcc gcttggcact
    25981 aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc gctatctggt gtttatgcca
    26041 ggggcttctc acgttggggt ttcccaacgt attgaaagcg aatcagaacg tgaacgcctg
    26101 aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt ttatcatccg taccgcagcg
    26161 gaaggggttg gcgaggctga actggcctcc gatgccgctt atctgaaacg cgtctggacc
    26221 aaagttatgg agcgtaaaaa acgcccgcag acccgttatc agctgtacgg cgaactggcg
    26281 ctggcgcagc gtgttctgcg tgatttcgcc gacgccgaac tggaccgcat tcgcgttgac
    26341 tcacgcctga cttacgaagc attgctggag tttacctcgg agtacattcc cgagatgacc
    26401 agcaagctgg agcattacac cggacgccag ccgattttcg atctctttga tgtcgaaaac
    26461 gaaatccagc gagcgctgga acgcaaagta gaactgaaat ccggtggtta tctgattatc
    26521 gaccagaccg aagcgatgac caccgtggac atcaataccg gggcgtttgt cggtcatcgc
    26581 aatctggacg acaccatttt caataccaat attgaagcga cgcaggctat cgctcgccag
    26641 ttacggttgc gcaatctggg cgggattatc attattgatt tcatcgatat gaataatgaa
    26701 gatcaccgcc gacgcgtgct gcactcgctg gagcaggcat tgagcaaaga ccgggtgaaa
    26761 actagcgtta atggtttttc ggcgctgggg ctggtggaga tgacgcgtaa acgcacccgc
    26821 gaaagcattg agcacgtact gtgtaacgaa tgcccaacct gccacggtcg cggaacggtg
    26881 aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga ttgttcgtgt ccaccatgct
    26941 tacgactccg accgtttcct ggtctatgct tctccggcag tagctgaagc cttgaaaggc
    27001 gaagagtcac actcgctggc ggaagtggaa attttcgttg gtaaacaggt taaagtacaa
    27061 attgaaccgc tctataacca ggagcagttt gacgtcgtaa tgatgtaaac agatgctggg
    27121 ccgccatccg gcaaagggtt tttgagtcac atttttagca gacaaggagt gacgggtgag
    27181 gcgattgccg gggattttac tgcttactgg agccgcgctc gttgtgatcg ctgccctgct
    27241 ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc tggcgtccgg aaatcctcaa
    27301 caaaatagaa tccgcgactg gcatgccggt agaagccagt cagctctcag ccagctggca
    27361 gaattttggc ccgacgcttg aagcacacga catccgtgca gaactaaaag atggcggcga
    27421 attttcggtt aaacgcgtta ctctggcgct ggatgtctgg cagagcttgt tacatatgcg
    27481 ctggcagttt cgcgacctca ctttctggca gctgcgcttt cgcaccaaca ctcctatcac
    27541 cagcggtggt ggtaatgata gcctggaagc cagtcacatc agcgatctgt ttcttcgtca
    27601 atttgaccat ttcgatctcc gcgacagtga agtcagtttt ctgacgccat ccggtcagcg
    27661 cgccgagctg gcgatcccac aactcacctg gctgaacgat ccacgtcgac accgtgcgga
    27721 aggcctggta agcctctcca gccttaccgg acagcacggc gtgatgcagg tgcgcatgga
    27781 tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc tggctacagg cggatgacat
    27841 cgacctgaag ccgtggctcg gtaaatggat gcaggacaat attgctctgg aaacggcaca
    27901 gttctccctt gaaggctgga tgacgatcga caaaggcgat gtaaccggcg gtgacgtctg
    27961 gctgaaacag ggcggtgcca gctggttggg cgagaagcaa acgcatacgc tgtcggtgga
    28021 taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg cagttctcta ttcccgatac
    28081 acggatcacg atggacggca aaccctggcc gagcggagca ttgacgctgg cctgggtacc
    28141 ggaacaggac gttggcggca aagacaataa acgcagtgac gaactccgga ttcgcgccag
    28201 taatctggag ctggcaggcc tggagggcat acgcccgctg gccgcgaaac tttcacctgc
    28261 actgggtgat gtttggcgct ccacacaacc gagcggcaag attaacactc tggcgctgga
    28321 tatcccgctt caggcggcag acaagacccg ttttcaggca tcgtggagcg atctggcctg
    28381 gaagcaatgg aaattattac cgggtgcgga acacttctcc gggacgcttt ccggcagcgt
    28441 tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag atgccttacg aaacggtatt
    28501 ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata agctggctga acaatgacaa
    28561 aggtttccag ctggatgggc gtaatattga cgttaaagcc aaagccgtcc atgcgcgcgg
    28621 cggttttcgt tacctgcaac ctgctaacga tgaaccctgg ctgggtattc tggctggcat
    28681 cagtaccgat gatggttcac aagcctggcg ctatttcccg gaaaacttga tgggtaaaga
    28741 cctggttgat tacttaagtg gcgcgattca gggcggtgaa gcggataacg cgacgctggt
    28801 ttatggtggc aatccgcaac tcttccccta taaacacaac gaaggtcagt ttgaagtgct
    28861 ggtgccgctg cgcaacgcga agtttgcctt ccagccggac tggcctgcat taactaacct
    28921 tgatattgaa ctggacttta ttaacgacgg tttatggatg aaaaccgatg gcgttaatct
    28981 gggcggcgtg cgcgcgagta atctcaccgc agtgatccct gactactcga aagaaaaact
    29041 gctgattgac gctgacatta aaggtccggg taaagccgtt ggcccttact ttgatgagac
    29101 accgctgaaa gattctctgg gggcgaccct gcaagaactc cagctcgacg gcgatgtgaa
    29161 tgctcgctta catcttgata tcccgctgaa cggcgaactg gtaaccgcga aaggtgaagt
    29221 gacgctgcgt aataacagtc tgtttatcaa accactcgac agcaccctga aaaatttgag
    29281 cggtaaattc agctttatca atagcgatct gcaaagtgaa ccactgacag caagctggtt
    29341 taatcagccg ttgaacgtgg atttttccac caaagaaggg gcaaaagcct accaggtagc
    29401 ggtaaacctc aacggtaact ggcaaccggc gaaaaccggc gttctgcctg aagcggtgaa
    29461 cgaagcattg agtggcagcg tggcgtggga tggtaaagtg ggcattgatc tgccttatca
    29521 tgctggtgcg acctataaca tagagctgaa cggcgatcta aagaatgtga gcagtcactt
    29581 accttcaccg ttagccaaac ctgcgggtga accactagcg gtaaacgtta aggttgatgg
    29641 caatctcaac agctttgaat taaccggaca ggctggtgcg gataatcatt tcaatagccg
    29701 ctggttgctc ggtcaaaagc tgacgctcga tcgtgctatt tgggcggcag acagtaaaac
    29761 gctcccgccg ttgccggaac aaagtggcgt tgaactcaat atgccgccga tgaatggtgc
    29821 cgagtggctg gccctgttcc agaaaggcgc tgcggagagt gtcggtggtg cagcgagttt
    29881 cccacaacac ataacgttac gtacgcctat gttgtcactg ggaaatcagc aatggaataa
    29941 cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg gttgaagcgc aagggcgtga
    30001 aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg ctggcgaata tcaaatatct
    30061 ttattacaac ccgagcgtgg cgaaaactcg tggtgattca acgccgtcat cacctttccc
    30121 gacaacggag cgcattaact tccgtggctg gccggacgcc caaatacgat gcacagagtg
    30181 ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat atcaccattt ctggcgatac
    30241 gttaacgctg accaatggac tgattgatac tggtttctcg cggcttactg ccgatggtga
    30301 atgggttaat aatccgggga atgaacgtac ctcgctgaaa ggaaaactgc gcgggcagaa
    30361 aattgatgcc gccgcagaat tttttggtgt cacgacgccc atacgccagt cgtcatttaa
    30421 tgtggattac gatttacact ggcgcaaagc accctggcag ccagatgaag cgacgttgaa
    30481 tggcatcatt catactcaac tgggtaaagg cgaaattacc gaaatcaata ccggacatgc
    30541 tgggcaattg ctgcgcttat tgagcgtaga tgccctgatg cgtaagctgc gttttgattt
    30601 cagagacact tttggcgaag ggttctattt tgactccatt cgcagcaccg cgtggattaa
    30661 agacggcgtt atgcacaccg acgacacgct ggtggatggc ctggaggcgg atatcgccat
    30721 gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg gaagcggttg tcgcaccaga
    30781 gatttctgcg acggtgggcg tggctgcggc ttttgcggtt aaccccattg ttggcgcggc
    30841 agtgtttgcc gccagtaaag tgctggggcc gctgtggagc aaagtctcca ttttgcgcta
    30901 tcacatttcg ggtccgctgg acgatccgca aatcaacgaa gtgttgcgcc aaccgcgtaa
    30961 agaaaaagcg caatgatttg acgagggcgc gtaattgccc caatctcata tgataatcgt
    31021 tgccaaaggc caacgagccg gaacataact gtaggccgga tagggcgttc acgccgcatc
    31081 cggcagccgt aaaaaatcct ctactgcagt aactaacgag tagcaaaaac gatgagtctg
    31141 aacctggtaa gtgaacaatt gctagcggcg aacggcctga aacatcagga cttgttcgcg
    31201 atcctcggtc aactggccga acgtcgcctt gattatggcg atctctattt tcagtcgagc
    31261 tatcacgaat cctgggtttt agaagaccgc attattaaag atggttctta caacatcgat
    31321 cagggcgttg gtgtgcgtgc aatcagtggt gaaaaaaccg gatttgctta cgcagaccaa
    31381 atcagcctgc tggcgctgga acagagtgcg caagcggcgc gcaccatcgt ccgtgatagt
    31441 ggtgatggca aagtacagac gctgggcgcg gtagagcata gcccgttgta tacctcggta
    31501 gatccgctgc aaagcatgag ccgtgaagag aagctggata tcctgcgtcg cgtcgataag
    31561 gttgcccgcg aagcggacaa gcgcgtacaa gaagtgactg ccagcctcag cggcgtttat
    31621 gaattaattc tggttgcggc caccgacggc acgctggcgg cggatgttcg tccgctggtg
    31681 cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac gcgaacgcgg tgccagtggc
    31741 ggcggcggtc gttttggtta tgagttcttc cttgccgatc ttgacggcga agtccgtgcg
    31801 gatgcatggg caaaagaagc agtgcgtatg gcgctggtca atctttctgc cgttgctgca
    31861 ccagcgggca ccatgccggt agtacttggc gcaggttggc cgggcgtgct gttgcatgaa
    31921 gcggtaggtc acggtctgga aggcgacttc aaccgccgcg gcacttcagt atttagtggg
    31981 caggtcgggg agctggtggc ttcagaactg tgtaccgtgg ttgatgacgg cacgatggtc
    32041 gatcgccgtg gttcggtggc gattgatgac gaaggtacgc caggccagta caacgtgctg
    32101 attgagaacg gcattctgaa aggctacatg caggataaac tcaacgcgcg tttgatgggg
    32161 atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat gccgcgtatg
    32221 accaacacct atatgctgcc gggtaaatcg accccgcagg aaattattga atccgttgag
    32281 tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg atattacatc cggcaaattc
    32341 gttttctcca cttcagaagc gtatctgatt gaaaacggta aagtaacgaa gccggtgaaa
    32401 ggcgcgacgt tgattggttc cggtatcgaa accatgcagc agatttcgat ggttggcaac
    32461 gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag tttgccggtt
    32521 ggcgtgggcc agccaacgct gaaagttgat aacctgactg ttggcggtac tgcgtaataa
    32581 tttcattatt ttcagaagga taattaatct ttctacgtgc acgaacggtc ccctcgctcc
    32641 tctggggtta gggtgagggg aacccgttgg cacaggtttg tagaacgtaa cagtacaata
    32701 tgaattactt ctctttcccg cgcccgtgca tctcctgaaa caatttaccg acctcaacaa
    32761 aataatccgt cagcgagttg atcacgacct gtaccttcag cggcagctta tctttttcgg
    32821 tatataacgc ataaaccggg cgtggatctg actgataacg cggcagcagg atctccagct
    32881 caccgcgatt gatctcgttg atcacccaca tcagcggcac gtaggcgatc ccggcacccg
    32941 ccgttagcca gcgcaccagc gtcatcggat cattagtcac aaatctccct tgcgggatca
    33001 ggcgagtcga gatcccttcc ggtgcaatca gttcaaattc attgtcgggc cgcacgctgt
    33061 attcaagcca tgaatggcta cttaaatcgg cgggtttttc cggtatgccg tattgtgtga
    33121 gatagctttt cgcggcgcac accaccattg gcatcgcgcc aagacggcgg gaaaacaggc
    33181 tggaatcctg caacgcgccg acgcggatca ccacatccag accgtcggca atcaggtcgg
    33241 gggctggaat tccggtaacc agattgacgc tcaaacctgg gtattctttc agcattttgg
    33301 ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga acagccaatg cgtagcgtcc
    33361 cgatgggggt gttattgaag gcatacagtt gctcatgaac atcctgcact tcatgaagca
    33421 tacgacggca gccctggtag taaattctac cggcttcggt caggccaatg ctgcgtgtgc
    33481 tacggtttaa cagctttacc tgcaactcat cttccagttt tgacaccgtc tgactgatgg
    33541 acgaaacgct catctgtagc tgtctggcgg cggcggtaaa agagccaaat tcaactactt
    33601 tggcaaacac cgacatgcgt tttagtcgtt ccattattca ctctgactta aaagtgattt
    33661 agatcacata atatagataa cagcataaca gttacgctaa tatattaaat atcaatctac
    33721 agcaatgttg ctctcgcccg gctttccatg cccttctctg cggcgacaga tgctgaaaat
    33781 aataacgcct gctctctctt tacaaccaag gtcaacatga gtctgtttcc cgttatcgtg
    33841 gtgtttgggc tgtccttccc accgatattt tttgaattgc ttttatcact ggcgattttc
    33901 tggctggtgc gccgggtact tgtgccaaca ggtatctacg actttgtctg gcatccggcg
    33961 ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga tatcgcgact gttcgtttga
    34021 ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg gccatcacgg tcgtattagt
    34081 cattctggcc ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat ccccctggac
    34141 gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt ctggactcat
    34201 tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa ggacagatac tgttcaccat
    34261 cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa gccgatgttg cttattatca
    34321 ggtactggca caggagaaac gccaggaggc cggacgtcgt aaccgtctcg gtgtgcaggc
    34381 gatgtctcgc gaagagatcg accaggctaa caacgtacta caaacggttc tgcatcagtt
    34441 agcgaaagcg caggcgaccc gcgatctggc aaaactggat cttgaacgca cggtgatccg
    34501 cgcgccagca gatggctggg tgaccaacct caacgtctat accggtgagt ttattactcg
    34561 aggatcaacg gcggttgcgc tggtgaaaca gaactccttc tatgtactgg cctatatgga
    34621 agaaactaag ctggaagggg tgcgtccggg gtatcgtgca gagatcacgc cgcttggcag
    34681 taacaaagtg ctgaaaggga ctgttgatag tgttgccgca ggggtcacca acgccagcag
    34741 cacgcgtgac gacaaaggga tggcgactat agactctaac cttgaatggg tgcgtcttgc
    34801 gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa gagaacatct ggcctgcggg
    34861 caccactgct acagtggtgg tcactggcaa acaagatcgc gacgaaagcc aggattcgtt
    34921 cttccgtaaa atggcccatc gcctgcgtga gtttggttaa tcacgatggg tattttctcc
    34981 attgctaacc aacatattcg ctttgcggta aaactggcga ccgccattgt actggcgctg
    35041 tttgttggct ttcacttcca gctggaaacg ccacgctggg cggtactgac agcggcgatt
    35101 gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt attctggcgc tattcgttat
    35161 cgtggctttt tgcgcatcat cggcacattt attggctgta ttgccggact ggtgatcatc
    35221 attgcgatga tccgcgcacc attattgatg attctggtgt gctgtatctg ggccggtttt
    35281 tgtacctgga tatcctcgct ggtacgaata gaaaactcgt acgcgtgggg gctggccggt
    35341 tataccgcgc tgatcattgt gatcaccatt cagccggaac cattgcttac gccgcagttt
    35401 gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt gtgcgattat ggcggatttg
    35461 ctcttttctc cgcgatcgat caaacaagaa gtggatcgag agctggaaag tttgctggtc
    35521 gcgcaatatc aattaatgca actctgtatc aagcatggcg atggtgaagt tgtcgataaa
    35581 gcctggggcg acctggtgcg acgcaccacg gcgctacaag gcatgcgcag caacctgaat
    35641 atggaatctt cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa tacgctatcg
    35701 ctgacgctga ttacccaatc ctgcgaaact tatcttattc agaatacgcg cccggaattg
    35761 atcactgata ctttccgcga attttttgac acgccggtag aaaccgcgca ggacgtccac
    35821 aagcagctca aacgcctgcg gagagttatc gcctggaccg gggaacggga aacgcctgtc
    35881 accatttata gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg cggcgttatc
    35941 agtaacacaa aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc ggaagtcaaa
    36001 gtagagtcag ccgaacgtca tcatgcgatg gttaacttct ggcgaaccac actttcctgc
    36061 attctgggca cgcttttctg gctgtggacg ggctggactt ccggcagtgg tgcaatggtg
    36121 atgattgcgg tagtgacgtc actggcaatg cgtttgccga atccacgcat ggtggcgatc
    36181 gactttatct acgggacgct ggccgcgctg ccgttagggc tgctctactt tttggtgatt
    36241 atccctaata cccaacagag catgttgctg ctgtgtatta gcctggcagt gctgggattt
    36301 ttcctcggta tagaagtaca gaaacggcga ctgggctcga tgggggcgct ggccagcacc
    36361 ataaatatta tcgtgctgga taacccgatg actttccatt tcagtcagtt tctcgacagc
    36421 gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct tgtgcgggat
    36481 aaatcgcgcg acaggaccgg acgtgtactg cttaatcagt ttgtttctgc cgctgtttcc
    36541 gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc tcccggcact ttatcagcag
    36601 ctgtttttgc tgatgaataa gttcccaggg gatttgccga aatttcgcct ggcgctgacg
    36661 atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga ggatttatcg
    36721 gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga tatctgcccg tagcgatgat
    36781 aaacgtcgtc ggtactttgg ccagttgctg gaagaactgg aaatctacca ggaaaagcta
    36841 cgcatctggc aagcgccacc gcaggtgacg gaaccggttc atcggctggc ggggatgctc
    36901 cataagtatc aacatgcgtt gaccgatagt taagtcaaaa ccgacgccaa aagcgtcggt
    36961 tttttcatgg ctatacttag cgatgcacgg cagaactcgc cgcgaaacgt gacggtggca
    37021 acagatgaat atttatacct ttgattttga tgagattgag agtcaggagg atttttatcg
    37081 tgactttagc caaacctttg gtctggcgaa agataaggta cgcgatctcg actcactatg
    37141 ggatgtgtta atgaacgatg tcctgccgct accacttgag attgaatttg ttcatctggg
    37201 agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg cagaggaaga
    37261 gctggaaggg catttgcgtt ttaatgttcg tcattagcgc gaaaaaaagc ccccgaaccg
    37321 ggggcaatat cgtcggacaa gacgatgagg gtttatttgt acagttcagc cgtagcgtgc
    37381 caggtgtcac cgctacgagc ttcagtaatc tggtaggccg ttgcgccttt ctcttccgct
    37441 tttttgttca gcatttcacg catatccatt ggcgaagacg ccacaccact tacggatacg
    37501 gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca
    37561 ccgaaagaga gaacagaaag tacgcttaat gcagctacag tggttttgat tttcatgatt
    37621 tttacctcga cataatcttt tagctgggtc tttgttt
//
LOCUS       DAFAVF010000029        36867 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFAVF010000029 DAFAVF010000000
VERSION     DAFAVF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.029"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..712)
                     /locus_tag="LGK42_001435"
     CDS             complement(<1..712)
                     /locus_tag="LGK42_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK2657698.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDDCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRA"
     gene            complement(890..2539)
                     /gene="actP"
                     /locus_tag="LGK42_001436"
     CDS             complement(890..2539)
                     /gene="actP"
                     /locus_tag="LGK42_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBK2657699.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2536..2850)
                     /locus_tag="LGK42_001437"
     CDS             complement(2536..2850)
                     /locus_tag="LGK42_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBK2657700.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(3050..5008)
                     /gene="acs"
                     /locus_tag="LGK42_001438"
     CDS             complement(3050..5008)
                     /gene="acs"
                     /locus_tag="LGK42_001438"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBK2657701.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            5400..6836
                     /gene="nrfA"
                     /locus_tag="LGK42_001439"
     CDS             5400..6836
                     /gene="nrfA"
                     /locus_tag="LGK42_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBK2657702.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6881..7447
                     /gene="nrfB"
                     /locus_tag="LGK42_001440"
     CDS             6881..7447
                     /gene="nrfB"
                     /locus_tag="LGK42_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBK2657703.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            7444..8115
                     /gene="nrfC"
                     /locus_tag="LGK42_001441"
     CDS             7444..8115
                     /gene="nrfC"
                     /locus_tag="LGK42_001441"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBK2657704.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            8112..9068
                     /gene="nrfD"
                     /locus_tag="LGK42_001442"
     CDS             8112..9068
                     /gene="nrfD"
                     /locus_tag="LGK42_001442"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBK2657705.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            9088..10806
                     /locus_tag="LGK42_001443"
     CDS             9088..10806
                     /locus_tag="LGK42_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBK2657706.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10799..11182
                     /gene="nrfF"
                     /locus_tag="LGK42_001444"
     CDS             10799..11182
                     /gene="nrfF"
                     /locus_tag="LGK42_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBK2657707.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            11179..11775
                     /gene="nrfG"
                     /locus_tag="LGK42_001445"
     CDS             11179..11775
                     /gene="nrfG"
                     /locus_tag="LGK42_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBK2657708.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            12117..13430
                     /gene="gltP"
                     /locus_tag="LGK42_001446"
     CDS             12117..13430
                     /gene="gltP"
                     /locus_tag="LGK42_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBK2657709.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13508..14197)
                     /locus_tag="LGK42_001447"
     CDS             complement(13508..14197)
                     /locus_tag="LGK42_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2657710.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14291..16438)
                     /gene="fdhF"
                     /locus_tag="LGK42_001448"
     CDS             complement(14291..16438)
                     /gene="fdhF"
                     /locus_tag="LGK42_001448"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(16019..16021),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBK2657711.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16636..18102)
                     /gene="mdtP"
                     /locus_tag="LGK42_001449"
     CDS             complement(16636..18102)
                     /gene="mdtP"
                     /locus_tag="LGK42_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBK2657712.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(18099..20150)
                     /gene="mdtO"
                     /locus_tag="LGK42_001450"
     CDS             complement(18099..20150)
                     /gene="mdtO"
                     /locus_tag="LGK42_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBK2657713.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(20150..21181)
                     /gene="mdtN"
                     /locus_tag="LGK42_001451"
     CDS             complement(20150..21181)
                     /gene="mdtN"
                     /locus_tag="LGK42_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBK2657714.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASTMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(21200..21475)
                     /locus_tag="LGK42_001452"
     CDS             complement(21200..21475)
                     /locus_tag="LGK42_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657715.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(21684..23669)
                     /gene="yjcS"
                     /locus_tag="LGK42_001453"
     CDS             complement(21684..23669)
                     /gene="yjcS"
                     /locus_tag="LGK42_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBK2657716.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23922..24239
                     /locus_tag="LGK42_001454"
     CDS             23922..24239
                     /locus_tag="LGK42_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBK2657717.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            24308..24637
                     /gene="yjdP"
                     /locus_tag="LGK42_001455"
     CDS             24308..24637
                     /gene="yjdP"
                     /locus_tag="LGK42_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBK2657718.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDLERRQLEDEERR
                     MEDEYGR"
     gene            complement(24784..25542)
                     /gene="phnP"
                     /locus_tag="LGK42_001456"
     CDS             complement(24784..25542)
                     /gene="phnP"
                     /locus_tag="LGK42_001456"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBK2657719.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(25544..25978)
                     /gene="phnO"
                     /locus_tag="LGK42_001457"
     CDS             complement(25544..25978)
                     /gene="phnO"
                     /locus_tag="LGK42_001457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBK2657720.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25965..26522)
                     /gene="phnN"
                     /locus_tag="LGK42_001458"
     CDS             complement(25965..26522)
                     /gene="phnN"
                     /locus_tag="LGK42_001458"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBK2657721.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(26522..27658)
                     /gene="phnM"
                     /locus_tag="LGK42_001459"
     CDS             complement(26522..27658)
                     /gene="phnM"
                     /locus_tag="LGK42_001459"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBK2657722.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(27655..28335)
                     /gene="phnL"
                     /locus_tag="LGK42_001460"
     CDS             complement(27655..28335)
                     /gene="phnL"
                     /locus_tag="LGK42_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBK2657723.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            complement(28446..29204)
                     /gene="phnK"
                     /locus_tag="LGK42_001461"
     CDS             complement(28446..29204)
                     /gene="phnK"
                     /locus_tag="LGK42_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBK2657724.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(29201..30046)
                     /gene="phnJ"
                     /locus_tag="LGK42_001462"
     CDS             complement(29201..30046)
                     /gene="phnJ"
                     /locus_tag="LGK42_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBK2657725.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(30039..31103)
                     /gene="phnI"
                     /locus_tag="LGK42_001463"
     CDS             complement(30039..31103)
                     /gene="phnI"
                     /locus_tag="LGK42_001463"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBK2657726.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            complement(31103..31687)
                     /gene="phnH"
                     /locus_tag="LGK42_001464"
     CDS             complement(31103..31687)
                     /gene="phnH"
                     /locus_tag="LGK42_001464"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBK2657727.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(31684..32136)
                     /gene="phnG"
                     /locus_tag="LGK42_001465"
     CDS             complement(31684..32136)
                     /gene="phnG"
                     /locus_tag="LGK42_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBK2657728.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(32137..32862)
                     /gene="phnF"
                     /locus_tag="LGK42_001466"
     CDS             complement(32137..32862)
                     /gene="phnF"
                     /locus_tag="LGK42_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBK2657729.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDNGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32883..33662)
                     /gene="phnE"
                     /locus_tag="LGK42_001467"
     CDS             complement(32883..33662)
                     /gene="phnE"
                     /locus_tag="LGK42_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBK2657730.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(33787..34803)
                     /gene="phnD"
                     /locus_tag="LGK42_001468"
     CDS             complement(33787..34803)
                     /gene="phnD"
                     /locus_tag="LGK42_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2657731.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(34828..35616)
                     /gene="phnC"
                     /locus_tag="LGK42_001469"
     CDS             complement(34828..35616)
                     /gene="phnC"
                     /locus_tag="LGK42_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBK2657732.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(35749..36192)
                     /gene="yjdN"
                     /locus_tag="LGK42_001470"
     CDS             complement(35749..36192)
                     /gene="yjdN"
                     /locus_tag="LGK42_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBK2657733.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(36352..36687)
                     /locus_tag="LGK42_001471"
     CDS             complement(36352..36687)
                     /locus_tag="LGK42_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBK2657734.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         8021 a         9845 c        10337 g         8664 t
ORIGIN      
        1 tggctctatt cattgctgca ttgtagaatt gaacatgcat agcggagcat gcatttagag
       61 ttccgtcggc catttttatc tgagcgaaat ttgagtcatc gagaatacaa tcatcaaaaa
      121 gtattttttc cattatggcg taataaaaat tcgttttata catattacag tcctggaaaa
      181 tagcgttttg tagtctgcaa cctgcaaaat tcgaattgga taaatcttca tctttaatca
      241 cgataccttt caggctaagg tgtgaaaaat cgtaaccacc atttactgtc tgtggaattt
      301 cgatagtatt attatggacg gcgacattat ttataaaata taccacctta atggatactt
      361 ccttttcttt actaaggtaa actcttctcg taattctatc gtcacattta ttatttgcat
      421 ataaagctat atctataaaa tgtttatgta ttttcctgtc cgtttccttc gtgatttttc
      481 ctgatggtaa actatcataa attcttttca ttttctgaat caaacctatc actttttcat
      541 gatcatccat cttgaaaaga gagagggctt tattgtcaat taacgtgttt ttatgatgtt
      601 tgctgtaatt atatgatttt gttttctttg tatgtgatga acttgtgaaa gagtggttat
      661 cgttaatctg gcaaagagag aaaaacatat tatttaaacc attatagcgc ataaaatatc
      721 cttataatta acaagaaaga aaaggcatat tctctgcatt atcattttct acgactgtca
      781 aaaatcgctc attttttaat gagtttattt gtttaatatt atgggaaaag gtgatgcatg
      841 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
      901 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgtgcacgg aacagttcac
      961 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
     1021 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccaa
     1081 ggatctgtac ccaaatcgtc gggccgagga tcatcagtac cactgcggta atcagcccca
     1141 gccagccacc catcatcgcg ccacgagtgg tcagtttcga ccagtacata gaaagcagaa
     1201 tgatcgggaa gttacagctc gctgcgatgg caaacgccag gcctaccatg aaggcgatgt
     1261 tctgattctc aaacagcacg ccgaggataa tggcaatcac acctaaaatc agcacggtga
     1321 ttttcgatac ccgcagttct tcacgttcgg tcgcgccttt tttgaagacg ttagcgtaca
     1381 ggtcatgcga aaccgctgat gcgcccgcca gcgtcagacc agcaaccacc gcaagaatgg
     1441 tggcaaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
     1501 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
     1561 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
     1621 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
     1681 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
     1741 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
     1801 tcgggtgtac cgccatcgct tcactgaaca gattgttgaa gctaaagccg acgtgtttca
     1861 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggctttg ataatctgta
     1921 cccaagtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1981 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccca
     2041 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     2101 gaatcggccc ttgtttcaga cggtaagagg ccacatcggc aaaggtgtag cgccccaggt
     2161 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     2221 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aagaatgagg
     2281 cggcggacat atagtccccg gcaatcgcca gcccgttctg gaagccagtg atattgccgc
     2341 ctgcggtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     2401 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     2461 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     2521 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     2581 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     2641 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     2701 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     2761 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     2821 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2881 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2941 aggccggata aggcgtttac gccgcatccg gcaatcaatg cctgatgcga cgctgtcgcg
     3001 tcttatcagg cctacaaacc gttaccgact cgcatccggc aattgtgggt tacgatggca
     3061 tcgcgatagc ctgcttctct tcaagcagct tctcgactac gccaggatcg gcaagcgtcg
     3121 aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt gcgcagaata cggcgcataa
     3181 ttttgccgga gcgggtttta ggcagggagt cggtccagtg cagcacgtct ggcgtcgcca
     3241 gcgggccaat ctctttacgc acccagttgc ggacttctgc gtacagttct ggtgacggtt
     3301 cctccccgtg attaagcgtg acgtaggcgt agatcgcctg acctttaata ttgtgcggaa
     3361 tacctactac ggcggcttcg gcaatcttcg gatgcgccac cagcgccgac tcaatctctg
     3421 ccgtccccag acggtgaccg gagacgttca gcacgtcgtc cacacgcccg gtgatccagt
     3481 aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa atacatattt ttgaaggtgg
     3541 agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag cgtacgcgcc tgacccggcc
     3601 aggagtcggt gatcaccagg ctgccttcgg tagcgccttc cagcgggtta ccttcgttat
     3661 cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc cgaaccggct ttcagctcgg
     3721 tagcgccagg cagcggcgtg atcatgaaac cgccagtttc ggtctgccac caggtatcga
     3781 ccaccggaca tttctcgttg ccgatttttt tccagtacca ctcccacgct tccgggttaa
     3841 ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg gtcggtaccc tcgatcgctt
     3901 tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc ggtatagaga atattgacct
     3961 gatgcttgtc caccacctgc gccatacggg caggcgtcgg ccagttgggt acgccttcaa
     4021 acatcagcgt ggtcgcaccg caggccagcg ggccgtacag caagtaactg tgtccggtca
     4081 cccagcccac atcggcggtg caccagtaga tatcacccgg atgataatca aagacatatt
     4141 taaaggtcag cgccgcgtac accagataac cgccggtagt atgcagcaca ccttttggct
     4201 taccggtaga accggaggtg tagagaataa acagcggatc ttcggcgttc atctcttccg
     4261 cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca ccacaggtcg cgcccttcct
     4321 gccagtcaat tttcccgcca gtacgcttca gtaccaccac atgctctacg ctggtgacgt
     4381 tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg aatactgcgc ccggcacgca
     4441 caccttcgtc ggaagtgatc accagtcgtg agttggaatc aataatgcgc ccggcaacgg
     4501 cttccggcga gaagccgccg aaaatcaccg aatgcaccgc gccaatgcgg gcgcaggcca
     4561 gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat cgccaccaca tcaccttttt
     4621 taatgcccag ctcgagcagg gtattggcga agcggcagac gtcgcggtgc agctctttat
     4681 agctgatatg tttgctctgg ctggcgtcgt cgccttccca gatgatggcg gtgcgatcgc
     4741 cgttttcttg cagatggcgg tcaaggcagt ttgccgccag attcagcgtg ccgtcctcgt
     4801 accatttaat ggacacatta ccgggggcaa aggaggtatt tttcaccttc tggtaaggtt
     4861 tgatccagtc gagaattttt ccctgttcgc cccagaaggt atcaggtacg ttaatagatt
     4921 gttgatacat cgcctcgtac tgctgagggt ttatcaggca acggtctgcg atgttggcag
     4981 gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc cttgtaggat gttaataata
     5041 tgtggcataa gcgttaaatg taggggtatt ggcagttttg tttagtattt gggcgacaga
     5101 tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa tttttgaagg tcagcaacaa
     5161 aagttgatta attctttgag gaacatgcag ttatgcatgc tgttgaaaaa gaggaagata
     5221 ctgactaact ctaaagtggt attttacatg cacttacaat tgattaaaga caacattcac
     5281 agtgtggtta tttgttacac ataggggcga gcaatgtcat gacagtgtag gtgcggttac
     5341 tgtcgtgaaa acaataaaaa ccgccattgc aacaatggcg caattcggat gaagccccta
     5401 tgacaaggat aaaaataaac gcacgccgta tcttcagctt attgattcct tttttctttt
     5461 tcacttctgt tcacgctgaa caaacggcag ctcccgcaaa acctgtaact gtggaagcga
     5521 agaatgaaac ctttgccccg cagcatcccg atcaatatct ctcctggaaa gccacctcgg
     5581 aacagtcaga gcgtgttgac gccctggcgg aagatccacg gctggtgatc ctgtgggcgg
     5641 ggtatccctt ctcgcgcgat tacaacaagc cgcgtggaca tgcctttgct gtgaccgatg
     5701 tgcgtgaaac cctgcgtacc ggtgcgccga aaaacgctga agatggtccg ctaccgatgg
     5761 cgtgctggag ttgtaaaagc ccggatgtgg cgcgtctgat ccagaaagac ggcgaagatg
     5821 gctacttcca cggtaagtgg gcgcgcggcg gcccggaaat cgtcaacaac ttaggttgtg
     5881 ccgactgcca taacaccgcc tcaccagagt tcgccaaagg caaaccggag ttaacccttt
     5941 cccgtccgta tgcggctcgc gcgatggaag ccattggtaa accttttgag aaagccggac
     6001 gtttcgacca gcaatcgatg gtttgcggtc agtgccatgt ggagtattac ttcgacggca
     6061 aaaacaaagc ggttaaattc ccgtgggatg acggcatgaa agtcgaaaat atggagcagt
     6121 attacgacaa aattgccttc tctgactgga ctaactccct gtcgaaaacg ccaatgctga
     6181 aagcgcagca cccggaatat gaaacctgga cagcgggcat tcacggtaaa aacaacgtga
     6241 cctgtatcga ctgccatatg ccaaaagtgc agaacgccga aggcaaactc tacaccgacc
     6301 ataaaattgg taatccgttt gataacttcg cccagacttg tgcgaactgc catacccagg
     6361 acaaagctgc cttgcaaaaa gtggtcgcgg aacgtaagca gtcgattaac gacctgaaaa
     6421 tcaaggttga agatcaactg gttcacgctc acttcgaagc gaaagcagcg ctggatgcag
     6481 gcgcgacgga agccgaaatg aagccaattc aggacgatat ccgtcatgcc cagtggcgct
     6541 gggatctggc gatcgcttcc cacggcattc atatgcacgc accggaagaa ggtttacgga
     6601 tgctcggtac ggcgatggat aaagcggcgg atgcacgcac caaactggcg cgcctgctgg
     6661 cgaccaaagg catcacccat gaaatccaga tcccggatat ctcaaccaaa gagaaagccc
     6721 agcaggctat tggcctgaac atggaacaaa tcaaggccga gaagcaggac ttcatcaaaa
     6781 cggtgatccc gcagtgggaa gaacaggcac gtaaaaacgg tctgttaagc caataacccc
     6841 gttccgcctc gcaaggggcg gaaaacacaa tggagtgaat atgagcgtat tacgttcgtt
     6901 attaactgcc ggggtgctgg cgtcgggcct gttgtggagc ctgaacggaa ttaccgctac
     6961 ccctgcggcg caggcatctg atgatcgcta cgaagttacc cagcagcgta acccggatgc
     7021 cgcctgtctg gactgtcata agccagatac cgaaggtatg catggaaaac atgcttccgt
     7081 tatcaacccg aataacaaac tgccggtcac ctgcaccaac tgtcacggcc agccatcacc
     7141 gcaacaccgc gaaggggtga aagatgtgat gcgctttaac gagccgatgt acaaggttgg
     7201 agagcagaac agcgtctgta tgtcctgtca tctgccggaa cagttgcaaa aagcgttctg
     7261 gccgcacgat gtccacgtaa ccaaagtggc gtgcgccagc tgccattccc tgcatccaca
     7321 gcaagatacg atgcagacgt taagtgacaa aggacggatt aagatttgcg tcgattgcca
     7381 cagcgatcag cgcaccaatc cgaactttaa cccggcgtct gttccgttgc ttaaggagca
     7441 gccatgacct ggtctcgtcg ccagtttctc accggcgtcg gcgtgctggc agccgtcagc
     7501 ggcaccgcag ggcgcgtggt ggcgaaaacg ttgaatatca atggggtgcg ttacggcatg
     7561 gtgcatgacg agtcgttatg catcggctgt accgcctgta tggatgcttg tcgggaagtg
     7621 aacaaggtgc cggaaggcgt ctcgcgcctg acgattattc gtagcgagcc gcagggcgaa
     7681 tttcctgatg tgaaatatcg cttcttccgt aagtcttgcc agcactgcga tcatgcgccg
     7741 tgcgttgacg tctgcccgac cggtgcgtct tttcgtgatg ccgctagcgg gattgtcgat
     7801 gttaatcctg atctctgcgt cggttgtcag tactgcatcg ccgcctgtcc gtaccgcgtg
     7861 cgctttatcc atccggtcac gaagacggcg gacaaatgcg atttctgccg taaaaccaat
     7921 ttgcaggccg gtaagctgcc cgcgtgcgtt gaggcttgcc cgacgaaggc gctgacgttt
     7981 ggcaatctgg acgatcctaa cagtgagatt tcgcaactgc tgcgccagaa gcctacttac
     8041 cgctacaagc tggcgctggg aaccaaaccg aagctgtacc gcgtaccgtt taaatacggg
     8101 gaggtgagcc aatgacgcag acttccgcat ttcattttga atcgctggtg tgggactggc
     8161 cgattgccat ctacctgttt ttgattggta tttctgccgg tctggtgacg ctggccgtgc
     8221 tgttacgtcg cttctacccg caggcgggcg gtgcagacag tacgttgctg cgcaccacgc
     8281 tgattgtcgg gccgggcgcg gtgatcctcg gtctgttgat cctcgtcttc cacctgacaa
     8341 gaccgtggac cttctggaag ctgatgttcc actacagttt tacctcggtg atgtcgatgg
     8401 gggtgatgct gtttcagctc tacatggtgg tgctggtgct gtggctggcg aaaatctttg
     8461 aacatgattt gcttgccctg caacaacgct ggttgccgaa gctggggatc gtgcaaaagg
     8521 ttctgagcct gctgacgccc gttcatcgcg gactggaaac attgatgctg gtgttggcgg
     8581 tgttgttggg ggcttatacc ggctttctgc tgtcggcgct gaaatcgtat ccgttcctca
     8641 ataacccgat cctgccggtg ctgttcctct tctccggcat ctcgtccggt gcggcggtgg
     8701 cgctgatcgc catggcgata cgccaacgca gtaacccgca ttccacggaa gcgcagtttg
     8761 tacaccgtat ggaaatcccc gtggtatggg gtgaaatctt cctgctggtg gcgttttttg
     8821 tcggtctggc gctgggcgat gacggtaaag tgcgtgcgct ggtggcggca ttaggtggcg
     8881 gtttctggac gtggtggttc tggcttggtg tcgccgggct ggggctgatt gtgccaatgt
     8941 tgctcaaacc gtgggtcaat cgcagttccg gcattcctgc cgtgctggcg gcgtgtgggg
     9001 ccagtctggt cggcgtgttg atgctgcgct ttttcattct ctacgccggg cagttaacgg
     9061 tggcgtaagc cagaaaagag gtggtttctg gacgtattcc ttcctgaagt cggttttctg
     9121 gcgttgttgt taagtctcgg ggtcaacgtg ttgaccccgt tgacggcctt tgcgggagtg
     9181 cggttgcgct ggcctgccat gatgcgactc acttgcatcg gcattctggc gcagttcgcg
     9241 ctcctgttgc tcgcctttgg cgtactgacg tattgttttc tcatcagcga tttctcggtc
     9301 atttatgtcg cccaacatag ctacagcctg ctgtcgtggg aactcaagct ggcagcggtg
     9361 tggggcggcc atgaaggttc gctgctgctt tgggtgctgc tgctttccgc ctggagcgcg
     9421 ctgtttgcct ggcattatcg gcagcaaacc gatccgctat ttccgctgac gctagccgtt
     9481 ttatctctca tgctcgccgc actgctactg tttgtagtgc tgtggtccga tcccttcgtg
     9541 cggatatttc caccagcaat cgaaggccgc gatctcaatc cgatgctgca acatcccggt
     9601 cttatctttc atccaccgct gctttacctt ggctatggcg gtttgatggt ggcggcgagc
     9661 gtggcgctgg cgagtttact gcgcggcgag tttgatggtg cctgcgccag aatttgttgg
     9721 cgctgggcac tacctggctg gagcgcatta acggcgggga tcatcctcgg ttcctggtgg
     9781 gcctattgcg aactgggctg gggcggctgg tggttctggg atccggtgga aaacgcctct
     9841 ttattaccct ggctttctgc cactgcgctg ctgcacagtt tgtccctgac acgccagcgg
     9901 gagattttcc gccactggtc gctgttgctg gcgatagtaa ctctgatgct gtcgctactg
     9961 ggtaccttaa ttgtccgttc tggcattctg gtttcggttc atgcgttcgc actggataac
    10021 gtccgcgccg tgccgttgtt cagcctgttt gcactgatta gccttgcgtc tctggctctg
    10081 tatggctggc gagcgcggga cggtggcccg gtggtgcgtt tttcggggtt atcgcgggaa
    10141 atgttaatcc tcgctacgct gttgctgttt tgcgcagtgc tactgatcgt gctggtggga
    10201 acgctttatc cgatgattta cggtctgctg ggctggggac gcctctccgt tggcgcgccg
    10261 tattttaacc gcgcgacgtt accgtttggc ctgttgatgc tggtggtgat tgtgctggcg
    10321 acgtttgtct ctggcaaacg cgtgcagctt ccggcgctgg tagctcatgc gggcgtgctg
    10381 ttatttgccg ctgggatcgt ggtttccagc gtcagccgtc aggagatcag cctgaattta
    10441 cagccgggtc agcaggtgac gctggcagga tacaccttcc gttttgagcg cctcgatctg
    10501 caagccaaag gcaattacac cagcgaaaaa gcgatagtgg cactgtttga ccatcagcaa
    10561 cgcattggtg aactgatgcc ggagcggcgt ttttacgaag cacgtcgtca gcaaatgatg
    10621 gaaccgtcaa ttcgctggaa cggcatccat gactggtatg cggtcatggg tgaaaaaacc
    10681 ggagcggatc gttacgcttt tcgcttgtat gtacaaagcg gtgtgcgctg gatctggggg
    10741 ggaggattgt tgatgattgc gggcgcattg ttaagcggat ggcgggggag gaagcgcgat
    10801 gaataaagga cttctcacgc tactgctgtt atttacctgt tttgcgcgcg ctcaggtcgt
    10861 agacacctgg caattcgcca atccgcaaca acagcaacag gcgttaaata ttgccagcca
    10921 gttacgttgt ccgcagtgcc agaatcaaaa cttactggaa tccaacgcgc cggtggcagt
    10981 cagtatgcgc catcaggttt acagcatggt ggcggagggg aagaacgaag tcgaaatcat
    11041 tggctggatg accgaacgct acggagattt tgttcgctat aacccaccgt taacgggtca
    11101 gacgctggtg ttgtgggcgc tgccagtggt gttgttactg ctgatggcac tgatcctctg
    11161 gcgagtgagg gcgaagcgat gaaagagccc aaaataccgg tgaaaatgct gacaacactc
    11221 acgattttga tggtatttct ctgtgtcggc agttatctgt taagtccaaa atggcaggcg
    11281 gtacatgcgg aatatcagcg tcagcgcgat ccgctacatc agtttgccag ccagcaaacc
    11341 ccggaagcgc agcttcaggc attgcaggat aaaatccgcg ctaatccaca aaacagcgaa
    11401 caatgggcgt tactgggcga gtactatctg tggcaaaacg attacagcaa ttcgctgctg
    11461 gcgtaccgtc aggcgttgca actacgtggt gagaacgctg aactgtatgc ggcgctggcg
    11521 acggtgcttt attaccaggc cagccagcat atgaccgccc agactcgcgc aatgatcgac
    11581 aaagccctcg cgctggactc taacgaaatc accgctctga tgctgctggc ttccgatgcg
    11641 tttatgcagg cgaattacgc gcaagccatc gaactatggc aaaaagtgat ggatctcaac
    11701 tcaccccgga tcaaccggac acagctggtc gagtcgatta atatggcgaa attgttgcag
    11761 cggagatcag attaatcatc tggtgatgaa gattatgtta ttgagttgta taaaaaataa
    11821 tcgaatgaac atatgccaaa aataatcacc aatcagtatt attgcagatt aaacaaataa
    11881 aaatctttcc ataacaaatg gttattcatt aatcctgcca taaactcgcg ttattatgca
    11941 ttaatgcagc gaaaagctct gttgttaaag ggttgcgcaa cataccgcgc aaatgatact
    12001 gatcataagc gttaaaaaaa tctacaaacc aacgcaacac aattcatgcc ctggcagtat
    12061 gtcacgttct cgcgtttctg aacggggaac ggcgctccat tgaggaagtt attctgatga
    12121 aaagtataaa attcagcctg gcctggcaga ttctgtttgc tatggtgctg ggcattctcc
    12181 tgggaagcta cctgcactac catagcgaca gccgcgactg gctggtcgtc aatttgctct
    12241 ctccggcggg tgatatcttc atccatctga tcaaaatgat tgttgtgccg attgtgatct
    12301 ccacgctggt ggtgggtatc gcgggtgttg gtgatgccaa acagctcggg cgtattggcg
    12361 cgaaaaccat tatttacttc gaggtgatca ccaccgtcgc catcattttg gggatcactc
    12421 tggcgaacgt cttccagccc ggtgccgggg tggatatgtc gcagctggcg accgtcgata
    12481 tctcgaaata tcagagcact acggaagcgg tacaaagcag ttcccacggc attatgggca
    12541 cgattttgtc gctggtgccg acgaacattg tggcgtcgat ggcgaaaggc gaaatgctgc
    12601 cgatcatctt cttctcggtg ctgtttggtc tggggctttc ctccctgccc gcgacgcatc
    12661 gtgaaccgct ggtgaccgtg ttccgctcca tctctgaaac catgtttaaa gtgactcaca
    12721 tggtgatgcg ttatgcaccg gtgggtgtgt ttgcgctgat tgcggtgacg gtggctaact
    12781 ttggtttctc ttctctgtgg ccactggcga aactggtgct gctggtgcat ttcgccattc
    12841 tgttcttcgc gctggtagtg ctgggaattg tggcgcgcct gtgcgggtta agcgtctgga
    12901 tcctgattcg tattctgaaa gatgagctga ttctggcgta ctccactgcc agctctgaaa
    12961 gcgtactgcc gcgaattatt gagaagatgg aagcctacgg agcaccggcg tcgatcacca
    13021 gcttcgtggt gccgaccggt tactctttta accttgatgg ttcgacgctg tatcaaagta
    13081 ttgccgctat tttcatcgct cagctgtatg gcattgacct gtccatctgg caggaaatca
    13141 ttctggtgct gacgctgatg gtgacctcga aagggattgc tggcgtgccg ggcgtgtcgt
    13201 ttgtggtgct gctggcaacg ctgggtagcg taggtattcc gctggaaggc ctggcgttta
    13261 ttgctggtgt tgaccgtatc ctcgacatgg cgcgtactgc gctgaacgtg gtgggtaatg
    13321 cgctggcggt gctggtgatt gccaagtggg aacacaaatt tgaccgcaaa aaggcgctgg
    13381 cttatgagcg tgaagtgctg ggcaaatttg ataaaactgc ggatcaataa ttgaagattg
    13441 ccggggttat ccacccggca atgtgtggat gcctgatgcg acgctggtgc gtcttatcag
    13501 gcctacctta accgttggtt aatttttcaa actcttcaca cccggtatca aacccttcca
    13561 tacagctcag gttcaaccag tgcagcgcct tttgtttgtt cttctcgata aaaccttcct
    13621 caccgtttaa gaacatcatt cccgcccagt actcggaata accggttcgg gaaattgcag
    13681 agctgcgttt gaaataccag gttgccttgt catcgtcggc cttaatgccc acgccgttgg
    13741 cgtaaatcaa accaagcagc atttgggcat cgacggcgga gtcattctcc agatcttccg
    13801 aggcgttttc taacagcgaa atcgcctttg gataatccgg tttacccgct tgagtattta
    13861 ccagaatatg cgccagcgtt acttcacctt ctttactgcc cgcttgcgcc gctttctctg
    13921 caagaacttt tgcttgtgga tagtccagac tgaccggatt ggtgattttg atctgcgcca
    13981 gcagcgcgca ggcatcagca tcaccgctgt cggcggcttt ctgtgcccaa tattcggctt
    14041 tgctcaaatc gccggagcta aaccagctgt cggcaagaaa atattgtgcg cgtcggtccc
    14101 cggcctctgc tgcctttaaa tactggctgc caggctcgga gtcgttcgcg tgggcaaaga
    14161 atgtcaaaaa caacatcaat gcgataattt ttttcatttt gataatacga ttgggtaggg
    14221 agtaaccagt ataaagagtt cgaatggata aaaaaacagc ctccgaaagg aggctgtaga
    14281 aaggactgta ttacgccagt gccgcttcgc gcaggcgagt tttcaacttg ttgtactcgt
    14341 caatcacgta ctgctcggcg gcgcgctgat cggcgatcgg ctcgacgcgc acggcgcagt
    14401 atttgtattc cggcgttttc gtaatcgggc ttaagttttc ggtaaccagc tcgttacagg
    14461 caccaatcca ccactggtag gtcatgtaaa tcgccccttt gttcggacga tcgctgacct
    14521 gcgcacgggt gataattttg cctttacgcg agtgcaccca aaccaatgcc tcatcttcaa
    14581 tacccagacg tttggcgtct tcggtattga tttgtgcgta gccaggttca tcagccagcg
    14641 ccgccagtgc cgcacagtta ccggtcatcg aacggcaaga gtagtgacca acttcacgca
    14701 ccgttgacag taccatcggg tactcgtcgg tgagtttgtc gattggcgct acccagtcgc
    14761 aggtgaagaa ctgtgccaga ccgttcgggg tatcaaactt ctctttaaac agataagaag
    14821 tcccctgatc ggcatctgaa gtatcgcggc aaggccactg aatgaagccc agttcgccca
    14881 ttttctcgta agtcgcaccg tagaaatccg ggcacagatg acgcaactca tcccagatct
    14941 cctgggtgtt gttgtagtgc atcggataac ccatacgggt ggcgatttca ctgatgattt
    15001 gccagtccgt tttcagatcc catttcggtt caactgcttt gaagaaacgc tggaagccac
    15061 ggtcagccgc agtaaacacg ccttcatgct cgccccacga cgttgacggc aaaatgacat
    15121 ccgccgccga cgcggttttg gtcataaaga tgtcctgaac gataaccagt tccagatctt
    15181 caaaggcttt acgtactgcc gacagctccg cgtcagtttg cagcggatct tcgcccataa
    15241 tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg cagctcgctg atgcgataac
    15301 cggtatgagc aggcaggctt tccacgcccc aggctttggc gaatttctcg cggttagccg
    15361 gatctttcac gtactggtat cccggatacg tatccggcag cgcgcccata tcgcaggcac
    15421 cctgcacgtt gttctgacca cgaaccgggt ttacacccgc atgcggctta ccgaggttac
    15481 cggtcagcat cgcgaggctg gtcagagaac gcacggtttc cacgccctgg tagaactggg
    15541 ttacacccat gccccacagg atggcggcgc ttttcgcctg ggcatacatc cgtgccgcct
    15601 gacgaatctc actggcgctg acgccggtga tatcttcaac cgactccggc gtgtagcctt
    15661 caacgatttt acgatactct tcaaagcctt ctgtacggga agcgacgaac gctttgtcgt
    15721 acagattttc ttcaataatg acatggccca tcgcattcaa cagcgcgatg ttcgagccgt
    15781 ttttcagtgc aatgtgcatg tcagcaatgc gcgcggtttc aattttgcgc ggatcgcaga
    15841 cgataatttt cgccccgtta cgtttagcgt taattacgtg attcgccacg attgggtggg
    15901 aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt attatcaatt tcgttaatag
    15961 cattgctcat tgcgccatta ccgaccgatt ggtgcagacc tgcaaccgat gggccgtgtc
    16021 agacacgagc gcagcagtca acgttattgg taccaataac ggcgcgcgca aatttttgca
    16081 ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc ggtcgtctgg atggcatccg
    16141 gaccgtactt ctctttgatg gcgctcaggc gctcggcaac gtaattcagt gcctcatccc
    16201 aggaaacagg ttcgagtttg ccgccacgct ggcgacggat catgggggtt ttcaggcgcg
    16261 gggtcaggat ctgggtatcg ttaatgaaat cccagccata ataacccttc agacacaggg
    16321 taccctggtt ggttttcccc tgcgctgcct ccgcccggac gattttgccg ttatcgacga
    16381 ccaggttgat tttgcaacct gatgcgcaat aggggcaaac cgtgacgact tttttcatcg
    16441 gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat gcatctttta
    16501 tgccacattt tatttggggt cattccctga tattacgggc actatttatt caaaactctg
    16561 acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg tgacagccat
    16621 cacgcggcag acattttatt ttttctcgac gacgggagcc gcctgatacc cgccgcccag
    16681 cgatttcatc aactgaatgc tttggatcac ccggcggctg tccagcatca gtaatgacat
    16741 ctcttcggca agtactggca accgggcttc agtggcctgt aagcggctgg ttaagccgcg
    16801 ctgataggcg gcctcggcag cgcgctgggt gaagcgcgtt gcttccacgc gttcagcctg
    16861 catttctcgc tcgtcgttga gcgtttgcag acgcgtgccg ttgacggcaa cgtcacgcac
    16921 cgcgttcagt actgactggt tgtaacgttc aatcatcatg ttgctggcgg cgcgcgtgcc
    16981 ttcgagattg gcattcaacc gtccaccgtc aaacagcggc aatttcagac ccgggatgaa
    17041 gttgaactgg cgactggttt ttttgaataa ggtatccagg tggatggcat caaggccgaa
    17101 aaacgctttg atatcaaagc tcgggtagaa cagcgcccgc gcggaatcca cctgatctaa
    17161 tgacgcctga acataccagc gcatggcttg cagatccggg cgtctggcga gcaactcata
    17221 agagagtgtc gccggaatgc cggtctggac tcgtggtaat gccaccggtt tgatctccgg
    17281 catatcgctg gcccccgcgc caatcaatgc acgcagagat tctcgcgttt cggtgatttg
    17341 ccctttgacg gcagcaattt gtttatcgac cgccagaatc tgtgcccgcg cgccgtggaa
    17401 aggcacttgt gcttccagac cgtgcgccac tttactctgg tgcgctttca ccgcgtaatc
    17461 aatcacatcg cgagtttgtt ctaacagatc gagcatctga tagctggcct gcatactgta
    17521 ataaagctgc gctacgcccg tggtcagcga tagctctact gctgcggttt ctgccagcgc
    17581 ggcattatgc gcgccaatgg cggcggcaac cgctgagcga tgcacacccc acaaatcaag
    17641 atccagtccg gcaaacaaac ctactgtggc ttccgtatag tacggcccgt ccatacccag
    17701 tgcgggcgca tccatcgcat aagggcttaa aaagccgttc gccgagacgc gttggcggtt
    17761 gagcatccct aacgctgcga cctgtaattg tgaaccggca tctaacaaat cggcctggga
    17821 ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg tgtgaaccac ttagcgtccg
    17881 ttggatcagc gcatccagct gcgggtcatt gagttgtttc caccactgcg cctgcggcca
    17941 gccggagctg gcaagatgaa tatcgtcggc cagtttgatt tgttccggtt tgagctgttg
    18001 atgaggtgca gaatccttac gtaccagggc acagccggaa atcagcgtcg tgctgccgag
    18061 aatgctgcac aacagcagac gtgaaagttg acgattgatc attgcgtggc tccttgtgcc
    18121 tgttccgtgg cgggcgttaa tgccggggct gtccagtctg gcaagcgggc gaaaagctgg
    18181 cagacgtgtt gctcctgttt taataaggtg ggcaggatgg cctgcgaggc gggtgtctct
    18241 tcaagcgtta tttgaggaga acggctgagt gcggttgcca gaccggcagc gtatttctcc
    18301 agggcgtcgg caaactgagc tgctctgtcc ggctgtagtg cgttatccag cgcctgcgcc
    18361 gagttccagg tggcatccca ggcgtgaaga atatcgcggc cctgacgaat aaccgtttgc
    18421 gaacgttcaa tcagtaaggc gcgttcttcg ctgtccagtt gacgctccag cgccacgcgt
    18481 tggcacatct cttcacaggc attaaacgcc gcatgcagac cgatacgaat ttgcagataa
    18541 gtgcgcagag ccgtgacttc ctgctgccgt gggatccgca ttactttact taacataccc
    18601 agcgcgccag ccagtttttg cggcagcgtg cgcgcttcac tttcaggcca gacaaaggtg
    18661 taaatcaccg cggacaccac ggtaccaatg atgataccca gggcgcgatc gcgaatttcc
    18721 accaggtcgt acactgggcc aaaaacgttt tcgagcgtgg cgagcgcgaa ggtgaccacc
    18781 atctgtgtgc cgatataaga agagcgttca gagctggtgg caatccatgc gcccaacagg
    18841 aaaatcgggg ccagcacaaa cagcaattcg acaatattgt ccagccaggg catgaccagt
    18901 agcgtgaata acagcgccag aatcgcgccg caaaaggccc cgccaaaacg cagcaccatc
    18961 ttctggtacg acgaaccgac atttgggtta gcgacgatca cgcatgtcag catacaggtg
    19021 tgaatgcctt cccagtccac gccgctgtaa aaggtgtaac agatcaaaca ggcgagcagc
    19081 gtttttaccg cgtagcgcat atagtctgga ttggtaaaag catcggcggc cattgatggc
    19141 ggtttggctg cgggcgttgg cggcgtattc gggtccatct gacccagttg taacaacgtc
    19201 tggcagatat tctccaggtt acattcccgt gccgccatcg cttcactttc actgatccgc
    19261 cagtcgcttt gccagcactg accttcagca acggcatgct gcagcttgtt gatttctgaa
    19321 gctaattttt gtcggaattc aataattgcc tgagaatcag caaaagaggt gggatcgtag
    19381 cgattcagcg tcgagtaaat gtaggttacc gttgccacgc agctttgcca ccatgcgctt
    19441 tgagttcgcc agttggcatc gtccgcgaga caaaagacat tcagtttttg cagcgccagc
    19501 gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc tgtccgtcag gtggctaatg
    19561 gcatcatcaa gccgatcatt aagcgcctga tgcatttgcg aaatggcacg actgggaaac
    19621 cacagcacgc cgattaacgt catcagcaag gttggataga ggccaacaac gatacaccac
    19681 agcgttaagc gcacgaccac ttccggatag tcgagcatgg cggggaaggt ttgcccgtaa
    19741 atggcgacaa tggcgactgc gaaaaagacc agtcccagcc gatgggtgcg catcaaaaac
    19801 atacagccca tcaggatcgg cccggcgatg atcaatcgga tcaacggttc gccgtatgac
    19861 catttgtaga tcaaaaacag gctgccgatc tccagcatcg tggcgaccac aaataagatc
    19921 gcgacaaatt tggtgtaaaa cgcgttcgac tgaataccgt aaaacagcac tgccagcgat
    19981 aacgccacga aagggatctc aaaggtcatc gagatcagaa tcaccagcag gcagcctacc
    20041 cagagttgca cggtctgcgg cacgcggcct ggtcgccgct cgcttaactc ttcatgaaag
    20101 aacgccagca gcctgaccac cggtaatggc agggagttga gcgcgctcat tattgcggct
    20161 caagattagc gactgccgaa gcgccgatgc ggaacatttc cgggtcaggt ttatcgacca
    20221 tgattttgac cggaaaacgc tgggcaacgc ggacccagtt aatagagcga gaaactttcg
    20281 gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac gccgtagcca atcgaatcca
    20341 ctttaccctc gaaggttttg ccgctgtcac tcatcaggcg aatcgttgcg ggtgtacctg
    20401 agcgaatatt tttcagatcg gtttcgcgga agttggcgat cacgtaccag tgacgagtgt
    20461 cgattagggt aaaaataggg cgcatcgtag atgcaaactg cccgacggag gttttgaggg
    20521 aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat ttccagatgc agtttggtca
    20581 gggcaatatc cgcttcgacc gccgcacgct gggcaactaa tgcatccacg ccgctgacgg
    20641 cgctggcggc tgactgcgcc tgcaacaata cagcattaag atcggcttct gcagcgcgct
    20701 gcgcggtcct cgcacggtca acgtcttccg ctgagacaaa accttctttc agtaatggct
    20761 cggtgcggcg taatgtatct gtggcctgtt tcgcggcggc acgggctttt tctaccgtgg
    20821 cgttaaccga gtcggcacca aactgttgcg cgtcaacgct acgctgggtg agcataattt
    20881 gcttatccag cgccgcgagg gaggcttcag ctttcgccag attggcttcg tacgggcgcg
    20941 ggtcaatgcg gaacagcaaa tcgccctgtt tgactgcctg gttgtcggtg accgccagtt
    21001 ctacaatgcg accgctgact tccggcacca catcaatggt atctgctgac gcgtaagcgt
    21061 cattagttga tggcgcactg tctacgcgcc agataacgaa aacaagggca accagcgcca
    21121 acgcaaccac taacagagca gggaatttac tgcgaggagc ttttttcggc gtactttcca
    21181 tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa cagcatggcg tagagagcaa
    21241 aaagggcggt ataaataatt ccgacaaatg ccagattgat atttgcccgc tgaataactc
    21301 gcctcgtgat gagcgtcaaa ataaggctgg caatggcgca gaaaaaccag ctgggataat
    21361 aagcgccgat caccgggatt gccggagata atgagcatcc cgaaaccatg aagacgggaa
    21421 ttatccaggc tgtttttttg agttgcatcg ccatgcgaga gagaactgtt ggcatattaa
    21481 atacatcctg tctgctgcga acaatatctg tagtgaatat caacatccat tgaaggcgtc
    21541 agtatggcta ttttcttgat gaataaaaat agctttattc agccaccgca ttcataactt
    21601 atatatttta tttattaatt ggctgtatat atttttaaaa taaatgcttt tgggggcttc
    21661 agcgccccca aaagcgagtg acattaattt ggggttacga tattcaccca caaatcgaaa
    21721 ttatccagac aggcgataat ttcttccagt ttcgcgccat tgccaataat tttggctttc
    21781 ttcgctttta ccagatccgc cattttggct tgtccggtca gcacggcgtg cagatcttca
    21841 cggctgatgt agaaagaggc gtcagcttgc ggttgcagcg ttttacggta gttaagcacg
    21901 ctatcgttca gcgtcaggtt gaggttatcg ccattgctca tattgaagtt caggctgata
    21961 tttttacccg cggctttcgc gctatcgagg cgaacggaca taaagtcgaa cagcatttcg
    22021 accgacatcc cgcgaatggt gtccggggaa ccggtggtgc cgtggctgaa cttatgcacc
    22081 ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc gccaggtggc ggactcggcc
    22141 tgatagccca gctgttcaaa gttattcgct tgcagattct tcgcgacctg gtcacccggg
    22201 ttggcggcaa tcacctgttt cagcagttct gccgaccagc ggtaatcacc ttgcttgttc
    22261 gcttcttgcg ccaggttaat aacgcgtgcg gaaccgccca gcgcctgcac gtaacgttta
    22321 cccatctcca cctgaccata cggatgcagg ttagccgggt taccgtcgta atagccaaga
    22381 tagaagttat acaccgcgcg ggcgttgtgg ctgacagaac cgtaatagcc acggctggcc
    22441 cagttattgg caagtgcagg cggcagttta atcatgtcgc cgatttcatt catggtgtag
    22501 ccctggttcg ccaggtgcag ggtctggtcg tgaatgtact tgatagtatc gcgatattta
    22561 ccaatataat cattgatatg cttattgccc cagaccggcc aggtgtgcgg cataaacagg
    22621 acttccgcgt cgttacccca catatccagc gtttcgttca gatactcggt ccacttgctg
    22681 gtgtcgcggg ttttcgcgcc gcgcagagtg tagaagttgt gcagggtatg cgtggcgttc
    22741 tcggcggtac acagcgcttt cagggccgga atatagaagt gcatttcggc tggtgcttcg
    22801 ctacctgggg tcatcaggaa gtcaaactcc aggccgtcga taatcatctt ctcaccggtt
    22861 ttggtgatgg ttttgttcgg tgcgatgatg ctcgggtagc ctgatgccag cgttacgcca
    22921 agaccattgc cgatgttacc ctgagggtta tgcgccagca gcaggccgta ggagtaaagt
    22981 gcacggcggc tcataatatt acccgccagt acgttttcgc tgatggcttc gtccataaag
    23041 ccagcagggg cgataacctg cactttaccg gatttcacat cggcttcaga gataatgcct
    23101 ttcacgccgc catagtggtc ggtatggctg tgggtataaa taaccgcaac aatcggtttt
    23161 tgcgggcgat tttggaagta aaggtcaagg gcggcttttg cgctcggtgg cgtcaccagc
    23221 gggtcgatga caataatccc ggtatcacct tcgataaagg tgatgttgga gatatcctga
    23281 ccgcgaacct ggtacattct gtcggtgact ttaaacaggc cggaaatacc gtttaactgc
    23341 gactgacgcc acaggctagg gttaacggtt tcgggcgcgg gcgcattgat atcaaattta
    23401 taatcttccc cgcgataata tggtttgcca ttagcatcgc gcagaatacc ttcatccagc
    23461 agcggagcga taaatccacg ctgagcgtcg tcaaaatcct gacgatcgct aaacggtaat
    23521 gatttggcgt aattctgatt aacctgttgg gtatattgag tggcggcttt cgcttcatct
    23581 gttgcgtaag cggtatttac catcatgccg ctggcagcag ttaacgcaat aacaatcctg
    23641 ctcaaacgga ataaccgaga gttattcata taaatctcca tttatcattt atgtcgtaaa
    23701 tatgtaatcc atgcaaaata cgtcatttaa agctggggtt atcataattg ggaattttcg
    23761 actttaataa gtggaagtgt gagcggaacg atccatttta ttaggcattt gtgctgaagg
    23821 gggaaaatta cgcgaattta ttatctctgt aaataatatg aggtgtttta tatatttatt
    23881 atatatattg tacttcggca aatactgagc acaatttatc aatgcaaccg gtgtttcttt
    23941 ttaaaaaacg taccggagca atgggcggtc atgtcgctgg caccgcagga ttctgcgttg
    24001 ctgtgccagc cttttattca tggtcaatga gtgaggtgat tgataaagac accaggtcgt
    24061 cagagcgcac tgattatccg cattatgcca gtgaagttgc actggctttt ggttcacgag
    24121 tggttggcct cgaactggca aaaatgattg tggatgcgtg gctgggcgca cagtacgaag
    24181 gcggtcgtca tcaacaacgc gtggaggcga ttacggcaat agagcagcgg agaaattgag
    24241 attcatccac tacttgcatg gatgagtaat gactaatgtg gatagagttt ctttttgagg
    24301 ttggctaatg aaacgctttc cgctatttct tttattcacc ctgctcacgc tgtccaccgt
    24361 tccggcccag gctgatatta tcgacgatac tattggtaat attcagcagg cgattaatga
    24421 cgcttataac cctgaccgtg ggcgagacta tgaagattcg cgcgacgacg gctggcagcg
    24481 tgaagtgagc gacgatcggc gcagacaata tgacgaccgc cgccgccagt ttgaagatcg
    24541 ccgcaggcag ctggacgatc gccagcgcca gcttgatctg gagcgtcggc aactggagga
    24601 tgaagagcgg agaatggaag atgagtatgg gcgatgaggt tgggtatgga gcaggcatcg
    24661 cccctcaccc caatggggag agggagaaaa cgagcgcaat attcaatatt gagcacaatc
    24721 ggtcccctcg cccctctggg gagagggtta gggtgagggg gccagcgcgc acattccctc
    24781 tcatcacgcc accccaatct ccatcccatc aaaccccacc tcaaaccctg acggtagtgc
    24841 gttttccatc agccacgcat caaactggtg gctgatatgg gtcagaatca cctgtggcga
    24901 gcggataacc tgattcagcg caagcacggt atttaaatca cagtgattac gcggtgcatc
    24961 cgcgcgcggc gggtgactgc aatcaatcac catcacctgc ggatgattat tgagtaaaaa
    25021 tttcagcgtt ttttccggca ggccagcggt gtcagacagc cacgccaccc ggctatgtgc
    25081 cgtttccagc agataaccga aggtcagttt tgagtggtta agcggcagcg gcgtgacctg
    25141 caacccctgc aaatcaaaca ccacaaacgg ttccactgtg tggctgaaat caagcaggcc
    25201 cggatgttta aacagatcgt cgcagccctg ttcatccggc gggccgtaga ccgggatcac
    25261 atcgcccacg ccccagcgta gcggaaatag cccttggacg tgatccatat ggtagtgcgt
    25321 cagcaaaaac tgctggaacg atccaggcga ccagcgatcg gtgagatcgt gccgcccggc
    25381 gtcgatcagg gtgatcgcgt cgttaaactt cactacgccg ctgcatggct ggcggcgata
    25441 ctgcggcgag cgccgcgctc tggcgcaggc cgcgcactcg cagccccatg ccggaacgcc
    25501 ctgtgcgccg ccagtgccgg tgagcgtgag ggtcaggctc atgttacagc gccttggtga
    25561 agcggaagtg gctctgctcg tagccttcac gcaggtaaaa acggtgtgcg tcgtggcgct
    25621 tcacgttggt ggaaagctcg gtcatttcgg ccccggcctg gcgggcttct tcttctgccc
    25681 atgccagtaa tttactgccg acgttcagac cgcgcgcctg cggcatcacc accagttcct
    25741 gaatttcgcc gatccagttg acatgatgca gatgaaactg caaatgcagg ccgatcatgc
    25801 cgacaacttc gccatccagc agcgccagat ggtagcgcat gtttgggtcg cgcaggttgg
    25861 cgttaaagcc cacgcgaaac gcatggtggt caaactccgc ctgttttagc tcacaaatca
    25921 gcgcgtaaac cgcgtcggta tcgtactgcg tggccgggcg aagctcacaa gcaggcatgg
    25981 tgtttctcct tctgatggat cagcgtcagc agcgtgtcga ccgactggcg caggctgccg
    26041 tcattgttga gcgtatggca atcctgtgga gtatagcggg cggcgcgcgc caggcgggcg
    26101 ttaatttcac tggcgttttc acggccacgg ttttccaggc gctggcgcag gatctccggc
    26161 gaaacctgta aacagacggg cagcagcgcc gattgatagc gcgcccgtgc ctgcggcaaa
    26221 tgcgcgcgtg aaccgttgac gactacgtcg aatccagcgt gcagccagag gtcaatctcg
    26281 atgccgacgc cataatacag accgttagcg tgccagctta aggccagcag gttttgcccc
    26341 gcgcgggtaa aaaactcccg ctcactcagg gcgatatggt tttcacttcc ggcgctggcg
    26401 tcgcgcgtga tgtagcgatg cgccaccagt aactgcgttt gttcccgcag gcggagttcc
    26461 gccagcaggc tgtctttccc ggagccggac ggccccatta accaaatcag ttttcccatc
    26521 atcagaacac ccttttaccc tgacgccaga cgtggtcgat atgaatgtga ttgccctggc
    26581 gatgcgccag caccaggtca gcgcgtttac cctcgccaat caccccgcga tcctgaagat
    26641 tcagcgcctg cgccggattt ttggtcacca gcctcaccgc ctgcggtagc gtaaagcggt
    26701 tgctctcgtc atcggcgacg cggaacgccg catccagcag gctggctggg tagtagtcgg
    26761 aagagaggat atccagcaga ccaagctgcg ccagttcact ggccgccaca ttgccggagt
    26821 gcgagccgcc acgcacgata tttggcgcac ccatcagcac gttcatgcca tgcttacgcg
    26881 aggcttccgc cgcttcgaac gtggtgggaa attcggcgat cacgctgcca agttggtggg
    26941 attcggcaac gtgggcgtgc gtggcgtcat cgtggctggc gagcgcaatt tgtcgcgcgc
    27001 ggcacagggc ggcgatggat tcgcgattcg gctgcgacca gcgtgcggcg agcgccaatt
    27061 gctcttcttc gtactgctgc atctgcgcat cggtgaggga gtatttgccc tgataatatt
    27121 cgcgatactt ctcgcggtta gcgaactggc gctggcccgg tgagtggtcc atcagcgaca
    27181 ccagcgtcac cggttcgcgc tgaaccagtt tttcaaacag cggcagtgtg gtgtgatgcg
    27241 gcagctcgca gcgcaggtgc agacggtgct cggcgcggtt gacgccgcgt ttctgcgtct
    27301 cttcgatggt gttgatcatc ttctccagat tctccagccg atcgccgccg tcgcgcacgt
    27361 cgccaatcgc caccgcgtcc agcacggtgg tgatgccgct tgccaccatc agcgcgtcgt
    27421 ggctgctcat cgccgaatgg gcgggccagt cgactttcgg gcgcggggtg aagaatttat
    27481 cgaggttgtc ggtatgcagc tcaatcaggc cgggtagtag ccagccacct tcgccgtcca
    27541 tcgcttctgg caggcggctc tggctttcgg caaaggcgcg gatttcgcca tcctgcacct
    27601 ccagcgaacc gtgtaccact tcgttttcca gcaccagttt gacgttattg ataatcatgc
    27661 agaggctccc attgggtgca agcggtcggc gacgtcatta cgtacggctt cgtcatggaa
    27721 gatgccgacg atggccgcgc cacgggtttt ggcttcgcga atcagctcga ctacggcggc
    27781 gctgtttttg gcgtccagcg aggcggtagg ttcgtcgagc agcagaatgg ggtagtcgac
    27841 gataaagccg cgggcgatgt tgacgcgctg ctgttcgcca ccggaaaagg ttgatggcgc
    27901 caggtgccac aggcgttccg gcacgttcag gcgggtgagc agacgcgcgg ctttagcggc
    27961 gcaggcttca cgcggaacgc cggtatcgag cagcggctgc atcaccactt ccagtgctga
    28021 aatacgcggg atgacgcgca gaaactggct cacccagccg acggtggttt tgcggatctc
    28081 caccactttg cgggctggcg cggtgaccag gtctacccac tcgtcaccgt gtttgatctg
    28141 gatttgacct tcatcgggca gatagttggc gtacagcgag cgcagcaggg ttgatttgcc
    28201 gctgccggag tgaccgtgga gcaccacgca ttcgcccgcg ttgacggtga gcgaggcgcg
    28261 gttgaggacg ggcaggcgca cgccgttttg ctggtgcagg atgaaggttt tactgacgtt
    28321 ttgtacgtta atcatttttc gcctcgtggt tcgggtgtcg gatgcgacgc tgacgcgtct
    28381 tatccgacct acggggagcg catttgtagg ccggataagg cgtttacgcc gcatccggca
    28441 ccggctcaat tctgcaaaac cgatgacacc agcagctgtg tatacggatg atgcgggtcg
    28501 tcgagcacgc ggtcggttaa cccactctcc accacttgcc cctgcttcat caccagcaaa
    28561 cggtccgcca gcaggcgggc aacgcctaaa tcatgggtga caatcaccac cgccaggttc
    28621 agctccacca ccaggccgcg cagcaggtcg agcaggcggg cctgcaccga cacatccagc
    28681 ccgccggtcg gttcatccat aaacaccagc ttcggatgcg tcaccaggtt gcgggcaatc
    28741 tgcaaacgtt gctgcatgcc gccggaaaag gtggtcggca ggtcgtcgat ccggttggcg
    28801 ggaatttcca cctcttccag ccacttctgc gcggtggcac gaatatcgcc gtaatgacgt
    28861 gccccggtcg ccatcagccg ctcgccgata ttgccgcctg ccgacacctg gcggcgcagg
    28921 ccgtcgagcg gatgctgatg cactacgccc cattcggtgc gcagcaggcg acggcggtcg
    28981 gcctcgctca ttgcgtacag cgaacggctc tcatagtgaa tctccccctg ctgcggcgtc
    29041 aggcgcgcgg agattgattt cagcagcgtg gtcttcccgg agccggactc cccgacaatg
    29101 cccagcactt cccccggcca taaatcaaaa gagacatcgc taaagccttt gcccggcgcg
    29161 taaaggtggg tcaggttatt gaccgaaagt aacggttgat tcattggctt ttcgcctcgc
    29221 tctgttggcg gcaataatcg gtatcggagc agacaaacat gcggtttccg gcgtcatcca
    29281 gcaccacttc atcaagatag ctgtgggtcg atccgcagat ggcgcatggc tcatcccact
    29341 gctgaacggt gaacgggtga tcgtcgaaat cgagactttc cacgcgggta aacggcggca
    29401 ccgcatagat gcgcttctcg cgccccgcgc cgaacagttg cagggcgggc atcatgtcca
    29461 tttttgggtt atcgaatttc gggatcggcg acgggtccat tacgtagcgc ccgttcacct
    29521 tcaccggata ggcgtaggta gtggcgatat gaccgaagcg ggcgatatct tcatacagtt
    29581 tcacctgcat cacgccgtac tcttccagcg cgtgcatggt gcgggtttcc gtttcgcgcg
    29641 gctcgataaa gcgcagcggt tccgggattg gcacctggaa gataatgatc tgatcttcgg
    29701 tcagcggcgt ttcggggatg cggtgacgcg tctggataag cgtcgcatcg tccgtacgtt
    29761 cagtggtgtt taccccggtc acgcgcttaa agaagttgcg aatcgacacg gcgttggtgg
    29821 tgtcatccgc accctggtca atcaccttca gcacgtcgct ttcgccaatc acgctggcgg
    29881 tgagctgtat gccgccggtt ccccagccgt atggcatcgg catctcgcgc ccgccaaacg
    29941 gcacctgata accggggatc gccaccgctt ttaagatagc gcggcggatc atgcgtttgg
    30001 tctgctcgtc gaggtaggca aagttgtagc cgctcagatt agccatggtt cttctcctgt
    30061 tgcagacgtt tgagtagctc cagttcggcc tggaaatcga cgtagtgggg gagtttgagg
    30121 tgcgagacaa agcctgcggc ttcgacgttg tcggcatgtg ccagcacaaa ctcttcatcc
    30181 tgcgccgggc ctgtcgcgtg ctcgccgtat tccggagcct gcaacgcacg atcgaccagc
    30241 gccattgcca tcgctttgcg ctcgctcatg ccgaatacca gcccgtagcc gcgcgtgaag
    30301 tgcggcggct catccggcgg gtcgataaaa ccgttgacca tttcacactc ggtcatcagt
    30361 agttcgccga cgtttaccgc aaatcccagc tcttccggca caatcgacac gtcgatgtaa
    30421 ccactgcgga tctcgcccgc gaacgggtga ttgcgcccgt aaccacgctg ggtggagtag
    30481 gccagcgcca gcaaatagcc ttcgtccccg cgcattaatt gttgcaggcg ggaggagcgt
    30541 gagcaggggt aaaccggcgg cgtgcgggtg atgtcatccg gctgtgcgcc gctatcttct
    30601 tcaaacttca ccagcccctg acgcgccagc aggctgaaaa cgtgcggcga cggctgttgt
    30661 tcgctgtcgg cggtggtcag cgtcggcgct tcaccgtttg ccagcagggt gaaatcgagc
    30721 aggcgatggg tgtagtcgta ggttgggcca agcagctggc cgccgggaat gtctttataa
    30781 accgccgaaa tacgccgttc gagacgcatc tctgtggtgt cgagcggctc gcttaccgcg
    30841 agcttcgcca gcgtggtgcg gtaggcgcgc agcaggaaaa ttgcttcgac gttatcgccg
    30901 ctggcctgtt tcagcgccag cgccgccagt tcgcggtcgg caatgccgcc ttcggtcatc
    30961 acgcgatcca ccgcaagatt aagctgctgt tcaatctggg cgacgctcag ttcaggcaaa
    31021 tcggtatcgc cccggcgtcg gctctcttgc agggcgtgag cggcgtcgat cgccttctcg
    31081 ccccctttca cggcaacgta catcagcaca cctccacatg agtggttcgc ggaatagcca
    31141 gcaggcgctc gccgcaggtc aggatcaggt cgatgccgag cgggaacggg tgcgggcgct
    31201 cggtgagttc gtgcagaatg cactccggca gctgcggagc gatcattcgt tcttcggcaa
    31261 tgcccgcgcc ggtgagacgt aacatgcgcc cgccgctcag gctggcgacc tgtaaaatca
    31321 gcgttgcacc cgcttccggc gcaacggcgg tgccggtgga aagggcgtta agctgttcgc
    31381 tggaaatcgc ctcatccgtc accgcgaagg tcgcctgttc cggctggctg accagcggcg
    31441 cgttggtatg aaaacgcagg ctctggctga cgatatcgtt actcaatggc gcggcaagcc
    31501 acaccggcgt gtcgttatcg gccagcgtca gcagcacgct ggtggtggcg atattcagcg
    31561 gctgccagcc gcgcttgagc tgatgcaggg cgacaatcac gcccggctcg ctcatggcct
    31621 ttaacaggcg acgaaaactg tgctgggcat cctgcacggg aagcataaaa gcggtttcca
    31681 gggtcatgcg ttgtctccgc gaaccatcgt aaagaagtcg acccggctgg cgttcacttc
    31741 ggcctggcgt gcggcaatgc gtgccatacg gtcagcgtcc agcggggcaa taagggtttc
    31801 tgataagttt tgaaaatagc gagattgctg catcagcgcg tcaatcagcg cgcagcgttc
    31861 ggcgtgctgt ttgtcgcgcc ccagcaccca gctgtagccg agcgtgccgt cagtcaggcg
    31921 tacggcggcg cgggtgagcg tggcgtcgcc ggcaaaaaag cgttcgccgg tgccgcccat
    31981 ccgcgcctga atctgcacca ggccggtttc ggcggcgcgg ataacctcat agtcggcggt
    32041 gatgttcagt gctttcaggc gggctgccag ttcagccggt tggctgtgcg ccagtacgga
    32101 catccagtgc tggcgggtcg cggtatctgc gtgcattcag tgctccatag tgaattcaat
    32161 catgtcggcg cgcgtcaggc tgacggagta ctccgccggg ctgctttcac cgtcacggtg
    32221 gttaagggtg cgcacgcaca gcagcggcga catattcgga atttcaagac gctggcactc
    32281 tttggcctgg gcgcgacggg cgctgatccg cgtctggctg cggcgcagcg caattccggt
    32341 ttgctcgcgc agaaaatcgt gcagcgaacc gttgtcgaag cgttgcagcg tcggccaaag
    32401 ggtgaggtcc gcaaagtagt ggtcgattaa acagagcgcg acgccgttga cgcgacgcag
    32461 ggtgcgcaaa tggataacgt tctcgccctc ggtaatcccc agcgcgtcag cgacgtgacc
    32521 ggacgcgggg cgcaataccg acagcagttt ttcgctggtg ggatggctac cctggtccag
    32581 caggttctgg ctaaaacgcg cctgggcgtt gagcgggtaa tcgaacgggc gcatcagcac
    32641 cagcacgccg acgccctgac ggcgctgtac ccagcctttt tccaccagtt ggtcgatggc
    32701 gcggcgcagg gtgtggcggt tcacctcaaa gcgcgccgcc agttgctgct cggcgggaag
    32761 atagtcgccg cagcggtagt gttgacgaag ctcctgctca agttttgcgg ctatctcctg
    32821 atagcgtgtt gggtagctgg tcggatgtgt agacaagtgc atagatatca atgcctcgct
    32881 tatcagataa agtgcttacg caaccgttga gagaggaaat ccagcaggct gaccgtgacg
    32941 atgataagca ccatcagggc gcaggtttgt tggaactgga aaccgcgaat cgcttcccac
    33001 agggtgacgc cgatcccgcc tgcgccgacc atgccgacga cggtcgccga gcggacgttg
    33061 gattcgaagc gatagaggga gtaggagatc agcagcggca tcacctgtgg cagcacgccg
    33121 tagaggatct cttcgagctt gttggcaccg gtggcgcgaa tgccttccac cgggccgggt
    33181 tcaatcgctt ccaccgcctc ggaaagcagc ttggagagca cgccggtggt gtggataaat
    33241 agcgccagca cgccagcgaa cggtccgaga ccgacggcga ccacgaacag catggcgaag
    33301 accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gaacgggctg gtaaacccac
    33361 cacggcacca ggttttcggc gctcatcagg ccgaagggga tggagagaac cactgccagc
    33421 gcggtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta atcctgccac
    33481 tggctgaaat cgggcgggaa gaagtcggca gcgaaggttg ccatgttgcc gccgtcttta
    33541 atcagcgtaa gcggggccat ttccgcgccc tgccacgaga cgaccagcac ggcgagaaca
    33601 acggcccagc tcagaagcga gaaccagcta cgcttgggtg gggcgatggt gatggtttgc
    33661 atgtttggct ccgtttaatg ttttctccct ctccctgtgg gagagggccg gggtgagggc
    33721 atcagcgtgc gcattccccc tcaccctaac cctctccctc aagggagagg ggaccgatcg
    33781 agcacattac tgcaccgctt tactcaccga actcatcgcg cttaacgcgt tgttcaggcg
    33841 gtccaggtca tccagctgcg cctgaatctc ggtggttttc gccagcttgt cctgctcatt
    33901 cagtccttta ttgcttttta cgccctgcat ctctttaaac agtgcgagct ggcgaatcgg
    33961 caccagttgc aggtcgctgg aagcgcggaa cggcgcccag cccaggcgtt ccagcaccgc
    34021 tttttcttcc ggcgttttgc cgtagttcat aaagaagtcg tagatcttgt ctttggtggt
    34081 ttcggaaagg tttttacgcc agacgatcgg atcgcctggg atcagcggcg acttccagat
    34141 cactttcagt tctttcaact tctccggcgc ggaggttttc agcttgtcga ggttttcggt
    34201 gttgttagtg gcaacatcca cctgcttgtt ggcgacggcc agcgcgttgg tttcatgccc
    34261 ggcgttgacg gtgcgcttga agtcgctggc ggagatattg tttttggcga agacgtagta
    34321 gccggggacg aggaagccag aggtggagtt aggatcgcca ttgccgaagg tgagatcttt
    34381 ccgcttcgcc agcagatcgt tcaggttgtt gatcggacta tctttgttga cgatcaacac
    34441 gctccagtaa cccggcgatc cgtccgccgc gacggtctgg gcgaagacct ggccgttggc
    34501 gcgatccacc gcttccattg ccgacaggtt gccgtaccag gcgatatcca ctttattgaa
    34561 gcgcattccc tggataatgc ctgcgtagtc tggggcaaag aaggcgttca ccttcacgcc
    34621 cagcttcttc tccatatcct gtaagaatgg cgtccattgc ggtttcaggt tttgctgtga
    34681 ttccgttgaa ataatgccga aattcagcgc cttttcctgc tcttcggcgt gtgccgggct
    34741 taacagggtg ctgaggctga acatgctggt gaaggccagc gaggcaatta tcttagcgtt
    34801 cattcgtttt cctcaatgat ggggatgtca ggcagctttc gcgttctctt cgatgcgatt
    34861 aatgctgcgg tagagatggt caaaacgttc gttatcaaac tgttggctgc tgccgtcgta
    34921 gaaaacgtgc ccctggcgca gggcgacgat gcgttcgcag tagcgcaggg cgtaatccac
    34981 ctgatgtagc gtgacgacca cggtgatgcc gtcgttctgg ttgatgtcgc gcagggtgtc
    35041 catcacgatg cgggcggatt ccgggtccag cgaggcgatg ggttcatcgg ccagaatcac
    35101 cttcgcctgc tgcatcagcg cgcgggcaat cgccacacgc tgctgctgtc cgccggagag
    35161 ggtggaaacg cgctgatggg caaaatgcac catgccaacg cgggtcagcg cctgtaacgc
    35221 gcgctgtttc tgctcgccgg tgaaccagct aaaacaggtg cgccagaacg gcgtgctgcc
    35281 gagcgcgcca atcagcacgt tctccagtac gctcaggcgg ttcaccaggt tgaattgttg
    35341 gaagatgtag ccggtgttgg cgcggctttt gcggatatcg cgcgccagac ggccttcgcg
    35401 ctggactgtg cggcccagca gctcgatatg gctgccggcg gatttatcgc cggtaatcaa
    35461 accgcttaag tgacgtaaaa gggtggattt gccggaaccc gacggcccaa gcagagccac
    35521 catttcaccg tgatgaatgt tcagatcaac cgcatgcagc gcctgatgct gattgaaggt
    35581 tttggcgagc ttctcgacac ggataatcgt ttgcatgatg cagcctccct aaaaaagtgg
    35641 ctccatcgtg gcggattatt gtgacgattt ggttaattaa aagtgactaa cagatgaaga
    35701 gttaacggga attcgatgac agtgcgggag ggcgggcctc ccggcggatt attgcgttgg
    35761 ttgttgtttg acgacattaa tcatccacgg tacgccaaat ttatcggtga ctttgccaaa
    35821 gccatgcgcc cagaaagttt cctgccaggc catttcgatt tttccgttag cggcaagatt
    35881 gtcaaaccag cgttttcctt cttcgacctg ttgcgaatcg agcaccagcg taaagccgga
    35941 gtagctggct tttcctgacg gcatggcatc gctcatcatg atgtcgcttc cggcaatgcg
    36001 cacgttggca tgagcgatgg cggtatcggg aaattgcatt ccggaagggc agttctcggc
    36061 gctgtcctgc gctgattttg gcatttcgcc gaagctgatt ttatagagca gttcagcgcc
    36121 caacgtacgt tgataataag caattgcgtc ggcacagtta ccggcaaaag agaggtaggg
    36181 acttaacggc atgatggtga cctcagttaa gagaagccag ttaagtgtag ttcgaaattt
    36241 atacagatga gagggaagcc tgataagacg cggtcagcgt cgcatcaggc agtcggcatc
    36301 gttgccggat gcggcgcgag cgccttatcc ggcttaccga tcacaataca atcagttctt
    36361 tttcacaaac tcagatttca gtttcatcgg accaaaaccg tcgattttgc aatcgatgtt
    36421 atggtcgcct tcaaccaggc ggatgttttt cactttggtg ccaattttca gcatcgaaga
    36481 gctacctttc accttcagat ctttaatgat ggtaacgctg tcgccgtcag ccagcagatt
    36541 gccgttagca tctttaacga tcagctcgtc gctttcctgt gcaggttctg cgtcgttcca
    36601 ttcgtaggca cattccgggc agatgtacat gccgttatct tcgtaagtgt attcggagtt
    36661 gcattttggg cagtgtggta atgacatgtg gatttcctca aaagtcagca gggcaaaagc
    36721 gccaaaaaac ggcagattgc cgaaaaaggc cgcaattata cacaaaatcc ctgagtttgt
    36781 cgggggtact gctgatttta gacgacgatc gttttcattt gaaaggaaac gcaaaaagaa
    36841 aataatcttt ttgcctctat ttattat
//
LOCUS       DAFAVF010000030        36567 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFAVF010000030 DAFAVF010000000
VERSION     DAFAVF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.030"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..1481)
                     /gene="ycjM"
                     /locus_tag="LGK42_001472"
     CDS             complement(<1..1481)
                     /gene="ycjM"
                     /locus_tag="LGK42_001472"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBK2657735.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFT"
     gene            complement(1693..2007)
                     /gene="pspE"
                     /locus_tag="LGK42_001473"
     CDS             complement(1693..2007)
                     /gene="pspE"
                     /locus_tag="LGK42_001473"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBK2657736.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2082..2303)
                     /gene="pspD"
                     /locus_tag="LGK42_001474"
     CDS             complement(2082..2303)
                     /gene="pspD"
                     /locus_tag="LGK42_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBK2657737.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2312..2671)
                     /gene="pspC"
                     /locus_tag="LGK42_001475"
     CDS             complement(2312..2671)
                     /gene="pspC"
                     /locus_tag="LGK42_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBK2657738.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2671..2895)
                     /gene="pspB"
                     /locus_tag="LGK42_001476"
     CDS             complement(2671..2895)
                     /gene="pspB"
                     /locus_tag="LGK42_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBK2657739.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2949..3617)
                     /gene="pspA"
                     /locus_tag="LGK42_001477"
     CDS             complement(2949..3617)
                     /gene="pspA"
                     /locus_tag="LGK42_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBK2657740.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3784..4761
                     /gene="pspF"
                     /locus_tag="LGK42_001478"
     CDS             3784..4761
                     /gene="pspF"
                     /locus_tag="LGK42_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBK2657741.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERAKETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDTIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4881..6146)
                     /gene="puuE"
                     /locus_tag="LGK42_001479"
     CDS             complement(4881..6146)
                     /gene="puuE"
                     /locus_tag="LGK42_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBK2657742.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(6184..7464)
                     /gene="puuB"
                     /locus_tag="LGK42_001480"
     CDS             complement(6184..7464)
                     /gene="puuB"
                     /locus_tag="LGK42_001480"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBK2657743.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7466..8953)
                     /gene="puuC"
                     /locus_tag="LGK42_001481"
     CDS             complement(7466..8953)
                     /gene="puuC"
                     /locus_tag="LGK42_001481"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBK2657744.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9228..9785)
                     /gene="puuR"
                     /locus_tag="LGK42_001482"
     CDS             complement(9228..9785)
                     /gene="puuR"
                     /locus_tag="LGK42_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBK2657745.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9812..10576)
                     /gene="puuD"
                     /locus_tag="LGK42_001483"
     CDS             complement(9812..10576)
                     /gene="puuD"
                     /locus_tag="LGK42_001483"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBK2657746.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10788..12206
                     /gene="puuA"
                     /locus_tag="LGK42_001484"
     CDS             10788..12206
                     /gene="puuA"
                     /locus_tag="LGK42_001484"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBK2657747.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12355..12519
                     /locus_tag="LGK42_001485"
     CDS             12355..12519
                     /locus_tag="LGK42_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657748.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12509..13894
                     /gene="puuP"
                     /locus_tag="LGK42_001486"
     CDS             12509..13894
                     /gene="puuP"
                     /locus_tag="LGK42_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBK2657749.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14028..14273
                     /locus_tag="LGK42_001487"
     CDS             14028..14273
                     /locus_tag="LGK42_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBK2657750.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14586..16229
                     /gene="sapA"
                     /locus_tag="LGK42_001488"
     CDS             14586..16229
                     /gene="sapA"
                     /locus_tag="LGK42_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBK2657751.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16226..17191
                     /gene="sapB"
                     /locus_tag="LGK42_001489"
     CDS             16226..17191
                     /gene="sapB"
                     /locus_tag="LGK42_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBK2657752.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17178..18068
                     /gene="sapC"
                     /locus_tag="LGK42_001490"
     CDS             17178..18068
                     /gene="sapC"
                     /locus_tag="LGK42_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBK2657753.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18068..19060
                     /gene="sapD"
                     /locus_tag="LGK42_001491"
     CDS             18068..19060
                     /gene="sapD"
                     /locus_tag="LGK42_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBK2657754.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19062..19868
                     /gene="sapF"
                     /locus_tag="LGK42_001492"
     CDS             19062..19868
                     /gene="sapF"
                     /locus_tag="LGK42_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBK2657755.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19936..20289
                     /locus_tag="LGK42_001493"
     CDS             19936..20289
                     /locus_tag="LGK42_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBK2657756.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            20659..21447
                     /gene="fabI"
                     /locus_tag="LGK42_001494"
     CDS             20659..21447
                     /gene="fabI"
                     /locus_tag="LGK42_001494"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK2657757.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21592..22719
                     /locus_tag="LGK42_001495"
     CDS             21592..22719
                     /locus_tag="LGK42_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBK2657758.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22787..24721
                     /gene="rnb"
                     /locus_tag="LGK42_001496"
     CDS             22787..24721
                     /gene="rnb"
                     /locus_tag="LGK42_001496"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBK2657759.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24799..24903)
                     /locus_tag="LGK42_001497"
     CDS             complement(24799..24903)
                     /locus_tag="LGK42_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657760.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            24957..26942
                     /gene="pdeR"
                     /locus_tag="LGK42_001498"
     CDS             24957..26942
                     /gene="pdeR"
                     /locus_tag="LGK42_001498"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBK2657761.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            27089..27262
                     /locus_tag="LGK42_001499"
     CDS             27089..27262
                     /locus_tag="LGK42_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657762.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            27352..28101
                     /locus_tag="LGK42_001500"
     CDS             27352..28101
                     /locus_tag="LGK42_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2657763.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            28370..28588
                     /gene="osmB"
                     /locus_tag="LGK42_001501"
     CDS             28370..28588
                     /gene="osmB"
                     /locus_tag="LGK42_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBK2657764.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28714..29040)
                     /gene="yciH"
                     /locus_tag="LGK42_001502"
     CDS             complement(28714..29040)
                     /gene="yciH"
                     /locus_tag="LGK42_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBK2657765.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29037..29777)
                     /gene="pyrF"
                     /locus_tag="LGK42_001503"
     CDS             complement(29037..29777)
                     /gene="pyrF"
                     /locus_tag="LGK42_001503"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK2657766.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIEPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSAW"
     gene            complement(29970..31139)
                     /gene="lapB"
                     /locus_tag="LGK42_001504"
     CDS             complement(29970..31139)
                     /gene="lapB"
                     /locus_tag="LGK42_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBK2657767.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(31146..31454)
                     /gene="lapA"
                     /locus_tag="LGK42_001505"
     CDS             complement(31146..31454)
                     /gene="lapA"
                     /locus_tag="LGK42_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBK2657768.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31603..32367)
                     /gene="pgpB"
                     /locus_tag="LGK42_001506"
     CDS             complement(31603..32367)
                     /gene="pgpB"
                     /locus_tag="LGK42_001506"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBK2657769.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32537..33127
                     /gene="ribA"
                     /locus_tag="LGK42_001507"
     CDS             32537..33127
                     /gene="ribA"
                     /locus_tag="LGK42_001507"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBK2657770.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(33191..35866)
                     /gene="acnA"
                     /locus_tag="LGK42_001508"
     CDS             complement(33191..35866)
                     /gene="acnA"
                     /locus_tag="LGK42_001508"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBK2657771.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(36030..36125)
                     /locus_tag="LGK42_001509"
     CDS             complement(36030..36125)
                     /locus_tag="LGK42_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657772.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(36239..36406)
                     /locus_tag="LGK42_001510"
     CDS             complement(36239..36406)
                     /locus_tag="LGK42_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657773.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(36409..36537)
                     /locus_tag="LGK42_001511"
     CDS             complement(36409..36537)
                     /locus_tag="LGK42_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBK2657774.1"
                     /translation="MPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKVWG"
BASE COUNT         8638 a         9539 c         9204 g         9186 t
ORIGIN      
        1 atggtaaaat tattatccgg atgaaactcg ttatggctgc gacgaagtgt aattaaacgc
       61 gacaactcat gatataccgc atgccttaat gtagcttcat cgttcagttc tcgggttatc
      121 tctttactgt gatatttttt acggttaatc gcacggttat atccgagttt ttcgacacct
      181 gcgtaatcat tacgcgagcc aagaatactt tgaatatata tcgctggcac accggggaaa
      241 cttaacaaaa tcgcatgggc gagaataaac ctggcgcaac gttcttcatc gctactctca
      301 cggcggctta acgcatccat ataggtcaca tttatttcat acggactgcg tgtaccgtcg
      361 ggattatttt tccagtttac taatgcacct tcctgctgta acgcctcgac cagctctaat
      421 atttcgcttt caggcaacaa gccccgtagc gggtttagcc cgatgccatc gtgagaggcg
      481 aggaagttaa accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa
      541 agcgcctcaa cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg
      601 tacaccatat gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga
      661 acattggtct cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac
      721 agtttgataa tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac
      781 ataaagccaa cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa
      841 acatccacca tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca
      901 ctaaaggtgg tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac
      961 ggtaacgcac gcgggcgagt gacggcgctg agatcggttt gcgggtcaac ggcaataaaa
     1021 aaatcttcaa aacctggatg ctgttgtaaa tagtttttaa accattcact ttttgccgac
     1081 atatggttgc agacaaaatc aaacattaaa tgactacatt caccgagttg ctgaatatcc
     1141 tgccactccc ccgcttcact ggcgacctgt tgataatcaa ttaccgaaaa gccatcatca
     1201 gatgaccatg gataaaacgg caacaaatga acatgtgaaa aaatgctttg cagccattga
     1261 tggtaaaact gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca
     1321 taggtaatta acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat
     1381 cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca
     1441 ccgtagattt cgtccaggta atccgtaatt ttctgtttca tgttgccttt ccgtggtagc
     1501 ggtcccatga aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt
     1561 ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa
     1621 taatctatgg ggatgtaaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca
     1681 tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt
     1741 ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc
     1801 ggcattgcaa tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc
     1861 tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc
     1921 tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc
     1981 cagagcaagt aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc
     2041 cattatacgc ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt
     2101 gccagtttat tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc
     2161 agcggtcggc gagcaactga ttttatcgcc cagcccgcca cacccgccgg gccatagcgc
     2221 agtgcggtaa gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg
     2281 gcctgttgcc agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg
     2341 tgaaagtatc ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca
     2401 gttcacggtc gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca
     2461 tgttgtccgg cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca
     2521 gcgccagacc gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat
     2581 caaaatagtt ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc
     2641 ataatttttt attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc
     2701 atcaagaata gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag
     2761 ttgcgctaat cgctgctgct cactttgcga caattcactg cgaccagaac gattgctgta
     2821 atgcagccat aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag
     2881 aaatagcgcg ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct
     2941 ggatgttatt attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg
     3001 attgcatcat cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag
     3061 ctgtggcttt ctgcttccgc ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga
     3121 gccattgctt catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt
     3181 gccgcctgat gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt
     3241 tccagctcac cgatctcttt cttcatgcgt gccagcgtgt cgtccaccag cgtcacttca
     3301 tgttccaggc tcttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc
     3361 aaatcctctc tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc
     3421 gccgacgctt gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa
     3481 gtagaacgta cttcaaccag tgtatcttcc atctcctgga tcatcagacg aaccagtttc
     3541 tgtggatctt ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag
     3601 cgagaaaaaa tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga
     3661 actgttaata caatttgcgt gccaattttt tatctttttg atttataaag atctgattga
     3721 agaatcaaca gcaacatgcc aggatgagtt agcgaattac actaacaagt ggcgaatttc
     3781 atcatggcag aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg
     3841 gaacaggttt cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc
     3901 accggtaaag agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg
     3961 tttatttccc ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt
     4021 cacgaagcgg gggcgtttac cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc
     4081 gacggcggta cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa
     4141 ttattgcgcg tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag
     4201 gtgaatgtgc ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc
     4261 acttttcgcg ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg
     4321 cgcgagcgcg aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg
     4381 gaaatcaagc tgcctctgtt cccggggttt acggagcgcg ccaaagaaac attgctgaat
     4441 tatcgttggc cgggaaatat tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc
     4501 cacggcacca gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg
     4561 cctgaagaca ctatcgccgt ttcagaaacc acctcgcttc caacactgcc gctggattta
     4621 cgtgagtttc agatgcagca ggaaaaagag ttgctgcaac tcagtttgca acaagggaaa
     4681 tataaccaga agcgcgcggc tgaattactg gggttaactt atcatcagtt ccgcgcgttg
     4741 ttgaaaaagc accagattta gcgaagttga gcaatacaac ttgccggatg cggcgtgaat
     4801 gctttattcg gcatacataa tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca
     4861 gacattattg cgttgggcta ttaatcgctc agcgcatcct gcaaaatttt cattgccgca
     4921 tcgaattgcg catccgggat ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg
     4981 ccacaggtca gcaggagcag cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc
     5041 gccgctgacg gctcgcccgt ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg
     5101 ccgcgtaccg cagcaatggc cggaacgctt tctttggcat caatcaacgt gtttttgaga
     5161 cgctggccca gttgattcgc gcgttcgcag agtgattctt tgtcgataat attgagcacc
     5221 gcgtgcgcgg cagccaccgc cagcgggttc ccggcgtagg tgccacccaa cccgcccggc
     5281 gcgggcgcgt ccataatatt cgcgttaccg accacgcccg aaagcggcat cccgcccgcg
     5341 aggcttttcg ccatcgtcat taagtccggc ttatcggcgt aatgatccat ggcaaacagt
     5401 ttaccggtac gcgcaaagcc gctttgcact tcatcggcaa tcatcacaat accgtgctcg
     5461 tcgcacaggc ggcgaatggc ggcaaccagc tcttttggcg caacgttgaa accgccctcg
     5521 ccctgcaccg gttcgaaaat aatggccgcg acctgcttcg cttcgatgtc tgatttaaac
     5581 aagcgttcga tggcgtcgag ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa
     5641 ggtacgtgat acaccgaacc agggaacggg ccgaagccga ttttgtacgg cgcaactttt
     5701 ccggtcagcg ccatggtcat atatgtacga ccgtggaagc caccgctaaa cgcaatcacg
     5761 ccaggtcgtc cggtatgggc gcgagcaatt ttcaccgcgt tttccaccgc ttccgcaccg
     5821 gtagtgaaga atgcggtttt agcctgcccg ctcactgggg caagggcgtt aattttctcc
     5881 gccagcgtga cgtagctttc gtacggcaca atctgatacg cggtgtgggt aaactgttgc
     5941 aattgctgct ccaccgccgc gaccagatca gggtggcgat gcccggtatt cagcaccgca
     6001 atgcctgcgg cgaaatcgat gtattcgttg ccctctacgt ccttcagcgt agcgttttct
     6061 gccgactggg cgaagaagtt acacatcacg ccaaccccgc gtggggtagc agaaagacga
     6121 cgctgatgga attcattgtt gctcatgttt cacacctttt gactcttcac tgttaatcgg
     6181 aaattaaacg cccagacgat cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg
     6241 cacgcgcagc gtgcgcccgc cggggaacgg gtaatgcggc agattggcga aggcatcgaa
     6301 acgttcggcg tcgccgcgca gcagttcggc aatcagacgc ccggcaagat gggtacaggt
     6361 gacgccgtgg ccgctgtagc cctgcatgta atagatgttg gtatcaaggc gaccgaactg
     6421 cggcatgcgc gacaaggtca gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc
     6481 cttcagctgc gggaaggttt tcagcagttt cggcaccaca aggcgctcaa cgtcatccgg
     6541 gtcgcgcgcg ccgtagacca cgccgccgcc gtacagcagg cggttgtcgg cggtaagacg
     6601 gtagtaatca agcagatagt tacagtcttc cacacagtag tttttcggga tcagcgaacg
     6661 ggctaaatct tccgacagcc gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg
     6721 tttcgccagt tccggctcta ctttatcgcc cagatacgca ttcccggcga caatcacata
     6781 cttcgccgtc acctgacctt tggcagttcg cacaacggct ggcgtggtgt gctggatctg
     6841 cgtcacggca gaaagttcat acacgcgccc gccgttgagg cggatggcgt ccgcttcgcc
     6901 aatggcgagg tttagcggat gaatatgccc accgctgtga tccagcagcg caccggtata
     6961 gcgatcgctg gctacttcac ggcgaatggc gttggcgtcc agcaattcca gctgtttatt
     7021 gccgtagcgt tcccagttct ctttctgctc ttcaagtgtg gcgagctgtt tatcattcat
     7081 cgccacaaac aggccgccgg ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc
     7141 gcggatgatc tcaccacctt cgaacatcat gctgccgagc atacgggcgg tgtccatgcc
     7201 gtagcttttt tcgatcacgt cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt
     7261 gcgcccgctt gcgccaaagc cgatgcgtga ggcttcgaga accactacgt caaagcccgc
     7321 ttccgccaga tgcagcgcgg aagagagccc ggtatagccg ccgccgacca cgcaaacgtc
     7381 gcaggaaatc gactcattca gcgtgtcgaa tggcgcgtat ttattcgcac tggcggcgta
     7441 gtaactgctg gtatgttcgg tcatttcagg cctccaggct tatccagatg gttttcagtt
     7501 cggtgaattt ttcaagggca tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc
     7561 cgccaaacgg cacggtcata tcgccgtcgt tgtagttatt gacgaagacg gaaccagcct
     7621 tcaggcggcg gctcatgcgg tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa
     7681 ggccgtactg gctgtcgttg gcaagctgta gcgcctggtc ttctgatgtg aaacgcgtga
     7741 ccaccagcac cggaccgaaa atctcttcgc ggcttaagga cgcattcggg tctacatcca
     7801 caaagatggt cgggccgatg gcggcagcca gctcagcgtt acgaccatcc agcagcagtt
     7861 gccctttgct ttcgccttcc cgaataaagc tatggaccga gtcggcgtgg gcgcagtcga
     7921 ttaaggtgcc catggtggtt gcgggatcaa gtggatggcc cggctgccag ttttgcgcct
     7981 gctgttttaa cagggctaag aattcatcgg cgatgctctc ttccagcaac aaacgcgttc
     8041 cggcgatgca cacctggccc tggttgtaga agatgcctgc ggcggtggcg cttgccgctt
     8101 tttgcagatc cggacaatcg gcgaaaacga ggttggcgct tttgccgccc gcttccagcc
     8161 agacgcgttt catgttgctg tcgcccgcat ctttcagcag ctgtttcccg gtacgggttg
     8221 aaccggtaaa ggcaatggcg tcgatatcgt tatgacgcga cagcgcctgc cccgcttcat
     8281 gaccaaaacc cgtcaccacg ttcaacacac catccggcaa gcctgcttct ttcgccagcc
     8341 ccgcgagacg aatcgcactg agcggtgatt tttcagacgg ttttagaatc acgctgtttc
     8401 ccgccgccag cgccgggccg agtttccagc aagtcagcaa cagcgggaag ttccacggca
     8461 cgatggcggc aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg
     8521 tggtcgccac ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg
     8581 ccgcgccggg aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt
     8641 ccagcagtgc cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg
     8701 ctttacgttt agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg
     8761 cgctcaccgc acggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct
     8821 gggtgaccgg atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt
     8881 taataaataa gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat
     8941 gatgaaaatt cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt
     9001 cagggcgcag agatccccgg gccgtatgcc cgtttatttc tggcgacatt ctttatatac
     9061 acttcatcct tcaagccgcc tctgccttgg ctgcgttcgc tcccccgaat cacttacctg
     9121 agtaagctca tcgggattcg ctcacttgcc gccttgatgc aacttgaatg attttgtgta
     9181 tagattgccg ggcgcattgc gcttacccgg cttacagaac aaaaagatta aaacgtggtg
     9241 ggcgtatggg cgctgataat tcggcaaata cctgccgacg tattactgaa actgtgcggt
     9301 atgccggtat taatggcata actttgcccc gcgacgaggt ggtaatcctg accattaatc
     9361 gtcagaacaa tttcaccttc cagtactgtg cctatttcct caccctgatg cttaattctt
     9421 tccccagtgg ttgtgcccgg ctggtacgtt tcaaagatca tcgccagcgt gcgattcggg
     9481 ttaccgttat gaaccagctt cattgacaca ccctgactgc ccatctcaat taagtcgtcc
     9541 tgattaatga cgacctgcgg ctcatcaggt ttttccggct cggaaaagaa ttccgagagt
     9601 gacagaccat acaccttcag cagcttttgc agcgtactga tggcagggct gactttatct
     9661 tgttctatcg tactgatagc actgtgagtc agcccggaga gttcggcggc acgacgttgt
     9721 gaaagcccct gctgctggcg gatttccgac aagcgttttc ctggcgccag tccctcatca
     9781 ctcatatttg catttcctta aactgtagtg gtcagagtcg ctgtttttca gcgatatggt
     9841 gctgacaagc ggtgataaag ccctcgaaca atatacgcga aagcgcgtac tcgctactgt
     9901 tccattccgg gtgccactgt acgcccagcg caaaaggatg attgatgacg ctaaccgcct
     9961 caactaagcc atccggcgag cgagcttcaa cacgcaaccg tgggctaacg accttcgccc
    10021 cttgcccatg cagagagttt acccaaaagt tgctacattc aggtaacaac gcagacagta
    10081 atcccccctc ttcaacctga acttcatgcg acggtgcata ttgctgttcc accggcagtt
    10141 cgggatcttc ccgatgttcc agcaattcag gctgttcgca cagcttgcga tgcaacgaac
    10201 caccggttgc caccaccagt tcctgtaaac cccggcagat ggcgaaaatg gggatgcgcc
    10261 tttcgagtgc ggcattaatt aacgccatgc tcagaagatc acgcccggga tcggcgtcag
    10321 gctcatcgcc gttttcacca tataggtgcg gctgcacatt gctgggacta ccaggaagat
    10381 aaatgccatc gagtttcggc aaaagttgtt caagtaatga cggttccgcc agcgcatgtg
    10441 gtagcgcaat tggcaagccg cctgcgtgga tgatggcatt caggtacttt tcttgcagag
    10501 tctgggtcgc atgaccctta agcctgttcc tgcacattac gacaccgata accggattgt
    10561 tcattatatt ttccatgctc aatctcacaa agtggactaa attatcgcca ttactgcggt
    10621 ttaaatgacc tttatgttca atattttttc aatctagcag tgggttttca tttttgcaaa
    10681 ctcaatttaa catttgacaa acatttagtt tgcatacaga ttcgaatggt ggtcattata
    10741 ttttacgctt tgataacgag cggaaaacaa accaaaggcg aagaatcatg gaaaccaata
    10801 tcgttgaagt agagaacttt gttcagcagt cagaagagag gcggggtagc gcctttacgc
    10861 aggaagtgaa acgctacctg gagcgctacc cgaatacgca atatgttgat gttttactta
    10921 ctgatttgaa tggttgcttc cgtggaaaac gtatccctgt ttcaagcctg aagaagttgg
    10981 agaaagggtg ttatttcccg gcctcggtgt ttgcaatgga tattctgggc aacgtagtag
    11041 aagaagcggg tctgggtcag gaaatgggcg agccggatcg tacttgtgtt cctgttctcg
    11101 gttccttaac cccttctgcc gccgatccag agtttatcgg tcagatgctc ctgaccatgg
    11161 tcgatgaaga tggcgctccc tttgacgttg agccgcggaa cgttcttaac cgcctgtggc
    11221 agcagctacg ccagcgcgga ttgttcccgg ttgtagcggt agagctggag ttctatttac
    11281 tggatcgtca gcgcgacgct gaagggtatc tgcaaccgcc ctgcgcgccc ggcaccgatg
    11341 accgcaatac acaaagccag gtttactccg ttgataacct caaccacttc gccgatgtgc
    11401 tcaatgatat tgatgaactg gcgcagttac agttaattcc cgccgacggt gcggtcgccg
    11461 aagcctcgcc gggtcagttt gaaatcaacc tttaccatac cgataacgtg ctggaagcct
    11521 gcgatgatgc gctggcgcta aaacgcctgg tgcgtctgat ggcagaaaag cataagatgc
    11581 acgccacttt tatggcgaag ccgtatgaag agcacgcggg cagcggaatg catatccata
    11641 tcagtatgca aaataaccgt ggcgagaacg tgctttctga cgcggaaggc gaagattcgc
    11701 cgctgctgaa aaagatgctc gccgggatga ttgacctgat gccgtcgtcg atggcgttgc
    11761 tggcaccaaa cgtgaactcg tatcgccgct tccagccggg aatgtatgtg ccgacgcagg
    11821 cgtcgtgggg ccataacaac cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata
    11881 atcaccgcgt ggaatatcgc gtggcgggtg ccgatgccaa cccatatctg gtgatggcag
    11941 cgatttttgc cggtattttg catggccttg ataacgagct gccgttgcag gaagaagtcg
    12001 aaggcaacgg gctggaacag gaaggcttac ccttcccgat tcgccagagc gatgccctgg
    12061 gtgagtttat cgagaatgat cacctgcgcc gctatttagg cgaacggttt tgccatgtgt
    12121 atcacgcctg taaaaatgat gagctgttgc agtttgagcg cctcatcaca gaaacagaaa
    12181 ttgagtggat gttgaaaaac gcgtaattcc ccatgcgggg ttgtttcatc ggcctgagcg
    12241 gagccaggcc tgacatttgc tggtaatcca gacatttccc ggatgtcgcg ttatggagcg
    12301 caggcggcaa cgggcttccg ttaacgatta agactgtgac gaggaaatga gatgatgccg
    12361 atgattaaat ccccgcatgg cgagggaggc tgcgtatgcg cccctcctgc tactgactgg
    12421 actccgcccc ctttgcttcc cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa
    12481 accttattac gcaggggagg cagcaattat ggctattaat tcaccactga atattgctgc
    12541 gcaacccggc aaaacccgtc tgcgaaaatc actgaaattg tggcaggtgg taatgatggg
    12601 tctggcctat ctcacgccga tgaccgtatt tgatactttt ggtattgtct ccggcattag
    12661 cgacggtcac gttccagcgt cctatttgct ggcgctggcg ggcgtgctgt ttaccgctat
    12721 cagctacggc aaactggttc gccagtttcc ggaggccggt tcggcctata cctacgcgca
    12781 aaagtcgatt aatccgcacg tcggatttat ggtcggctgg tcatcactgc tggattatct
    12841 ctttttgcca atgatcaacg ttttgttggc gaaaatctat ctctccgccc tcttcccgga
    12901 agtgccgccg tgggtgtggg tggtaacctt cgtcgccatt ttaaccgccg cgaatctgaa
    12961 gagcgtcaac ctggtcgcta acttcaatac cctgtttgta ctggtgcaaa tctccatcat
    13021 ggtggtgttt atcttcctgg tggttcaggg actgcataaa ggagaaggcg ttggcaccgt
    13081 ctggtcactt cagccgttta tcagcgagaa cgcgcacctg atcccgatta ttaccggtgc
    13141 gacgattgtc tgtttctcgt tcctcggttt cgatgcggtg accacgcttt cggaagagac
    13201 gccggacgcc gcacgcgtga tcccgaaagc catcttcctg acggcggtat acggcggcgt
    13261 tatctttatc gcggcgtcgt tctttatgca gctgttcttt cccgatatca gccgctttaa
    13321 agacccggac gccgcactgc ctgaaattgc gctgtacgtc ggcggtaagc tgttccagtc
    13381 gattttcctt tgcaccacgt ttgtgaacac gttagcgtct ggcctggcct cacatgccag
    13441 cgtgtcacgt ctgctgtatg tgatggggcg cgacaatgtg tttccggagc gcgtgtttgg
    13501 ctatgtgcat ccgaaatggc ggactccggc actgaacgtc attatggtcg ggattgtcgc
    13561 gatgtcggcg ctgttcttcg atttagttac cgccacagcg ttaattaact tcggcgcgtt
    13621 ggtagcgttt accttcgtta acctgtcggt gttcaaccac ttctggcggc gcaaaggaat
    13681 gaataaaagc tggaaggaac acttccacta tttattaatg ccgctggttg gcgcgctgac
    13741 ggtgggcgtg ctgtggatta acctcgaagc cacctcgctg acgctgggtc tggtatgggc
    13801 ttcgttaggc ggcgcatatt tgtggtatct gatccgccgc tatcgcaaag tgccgctgta
    13861 cgaaggtgac agaacgccag tgagcgaaac gtaatagtcg gatgcgcgcc gcattatcct
    13921 gcctgagggt aatgcgccgc gccattaact ataaaaagat gtatgacccg gatcacgagt
    13981 gctatcatcc gcccttatgc ttagcgcatt aaccccaggg gcaaataatg aaccacgata
    14041 tcccgctaaa atattttgat attgccgatg agtacgcgac cgaatgcgca gagcctgtcg
    14101 cagacgctga acgcacaccg ctggcccact acttccagtt actgctcacc cgtttgatga
    14161 acaatgaaga gatcagcgaa gaagcccaac atgaaatggc tgctgaagcg gggattaatc
    14221 ccgtacgcat tgatgagatc gcagagtttt tgaatcaatg gggtaatgag taattattta
    14281 attgcagacg atcggtcagt atcgttgtcc ggataaagcg ttcacgccgc atccggctaa
    14341 caatgacacg cactgcagcg tgctttaatg ggtttctcac gatcgtaccg ctctgctcct
    14401 cgctaagttg ttctgtgagt tatagttata gagaaatcac gggtacgaat gttgaacaga
    14461 tggcgacgcc acctgttcaa cgaccaacca cacggccctc attacgaccc ctgaacacaa
    14521 acggtaatgg caaatgtcat taaatattag taagatagcc tctttacata aaatccctta
    14581 atattatgcg ccaggtatta tcgtctcttt tggtgattgc tggacttgtg agtggtcagg
    14641 caatcgccgc gcctgaatcc cccccgcatg ctgatatccg cgacagcggt tttgtctatt
    14701 gcgtcagcgg gcaagtcaac acctttaacc catccaaagc gagcagtggg ttaattgtcg
    14761 atacccttgc cgcccagttt tatgatcgac tgctggatgt cgatccctat acctatcgcc
    14821 tgatgccgga acttgccgaa agctgggaag tactcgacaa cggcgcgacc tatcgcttcc
    14881 acctgcgtcg cgatgttccg tttcaaaaaa ccgactggtt tactcccact cgtaaaatga
    14941 atgccgacga tgtggtgttt accttccagc gaatttttga ccgcaacaac ccgtggcata
    15001 acgtcaacgg cagcaacttc ccctacttcg acagcctgca atttgccgat aacgtgaaaa
    15061 gcgtccgcaa actggataat cataccgttg agttccgact cgcccagccg gatgcttctt
    15121 ttttgtggca cctcgcaacc cattatgctt cggtcatgtc ggcagaatat gcccggaagt
    15181 tagagaaaga agatcgccag gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc
    15241 agttgtcgga ataccgcgcc gggcaattta ttcgcctaca acgtcatgat gacttctggc
    15301 gcggtaaacc gttaatgccg caggtagtgg tggatttagg ctccggcggc accggacgtc
    15361 tgtcgaaact cctgaccggg gaatgcgacg ttctggcctg gcctgctgcc agccagctat
    15421 ccattttgcg tgacgacccg cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct
    15481 atctggcatt taacaccgcc aaaccgccgc taaataatcc cgctgtccgc catgcactgg
    15541 cactggcgat taataaccag cgcctgatgc aatccatcta ttatggtacg gctgaaacgg
    15601 cggcctctat tttaccgcgc gcctcgtggg cctatgacaa cgaggctaaa attactgaat
    15661 acaatccggc gaaatcgcgc gaacagttga aggcgttggg gctggaaaac ttaacgctga
    15721 aactgtgggt gcccacacgt tcgcaggcgt ggaaccccag tccactgaaa actgccgaac
    15781 tgattcaggc ggatatggcg caggttggcg taaaagtggt gattgtgccg gtagaaggtc
    15841 gctttcagga ggcgcggttg atggatatga gccatgatct gacgttatcc ggttgggcga
    15901 cggacagtaa cgacccggac agtttcttcc gtccgttact gagctgcgcg gcaattcatt
    15961 cacagaccaa cctcgcccac tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc
    16021 tctcctcgca gcagctggca gcgcgtattg aagcctatga cgaagcgcag agtattctgg
    16081 cgcaggaatt acctattctg ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg
    16141 atatcaaagg tctggtactt agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg
    16201 agaaacagga tgaggtgaaa aaaccatgat tatcttcacc ttacgccgca ttttgttatt
    16261 gattgtcacc ttgttcctgc tgaccttcgt tggcttcagt ttgagctatt tcactccaca
    16321 cgcgccgtta caaggcgcgt cactgtggaa tgcctgggtg ttctggttta acggcctgat
    16381 ccactgggat tttggtgttt ccagcattaa cggtcaaccg attgccgaac agttaaaaga
    16441 ggtcttccct gcgacgatgg aactgtgcat cctcgccttc ggttttgcgc tgattgttgg
    16501 catccccgtg gggatgattg ccgggattac gcgccataag tggcaggata acttgatcaa
    16561 cgccatcgcc ttgctgggct tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct
    16621 gttttgttca ctcacgctag gttggttgcc cgtttcaggg cgtttcgatc tgctgtacga
    16681 agtgaaaccg attaccgggt ttgcgttgat tgatgcctgg ctttcggact caccatggcg
    16741 ggatgaaatg atcatgagcg caatccgcca tatgatattg cccgtgatca ccctgtcggt
    16801 tgcgccaaca actgaagtga tccgactgat gcgtatcagc actatcgaag tgtatgacca
    16861 gaactatgtt aaagcggcgg ctacccgcgg tttgtcgcgc tttactattt tgcgtcgcca
    16921 cgttctgcat aacgcgttac cgccggttat tcctcgcctg ggcttacagt tttccaccat
    16981 gctgacgctg gcgatgatca ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt
    17041 aattaacgcc atccgccagc aggattacgc agccatttcc gccggagtga tggtgtgtgg
    17101 ctcactggtc attattgtta acgtgatttc tgatattttg ggtgccatgg ctaaccctct
    17161 gaaacataag gaatggtatg ccttacgata gcgtatacag cgaaaagcgc ccgccgggca
    17221 cgctgcgtac cgcctggcgc aaattttata gtgacgcctc tgcgatggtc ggcctgtacg
    17281 gctgcgcggg gctggctgta ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg
    17341 accagcaatt tctcggttac caattattgc cgccgtcatg gtcgcgctat ggcgaagttt
    17401 ctttcttcct ggggactgac gacctcggac gcgatgtttt aagccgttta ctgagcggag
    17461 ctgcgccaac cgtgggtggc gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg
    17521 tactgggcac cttcgccggg gcgacgcacg gcctacgctc agcggtgctt aaccatattc
    17581 tggatacctt actggcgatc ccttcgctgc tgctggcaat tatcgttgta gcgtttgccg
    17641 gaccgagttt gtcccacgct atgtttgctg tctggctggc attgctgccg cgtatggtac
    17701 gttcgattta cagcatggtg catgacgaac tggaaaaaga gtacgttatc gccgcccgtc
    17761 tggatggcgc atcaacgctg aatattctct ggtttgctgt gatgccaaac atcaccgccg
    17821 ggctggtcac cgagatcacc cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg
    17881 gctttctcga tctcggcgca caactcccct cgcctgaatg gggagcgatg ctcggtgatg
    17941 cgctggaact gatttatgtc gccccgtgga ctgtcatgct gccaggtgcg gcaattatga
    18001 ttagcgtgtt gttggttaac ctccttggcg atggcgttcg tcgtgcgatt attgcggggg
    18061 tggaataatg ccattactcg atattcgtaa cctgaccatt gaatttaaaa ccggtgatga
    18121 gtgggttaaa gccgtcgacc gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct
    18181 tgttggtgaa tccggttccg gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa
    18241 agataactgg cgtgttactg ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct
    18301 ctccgcacgc gaacggcgca aactggttgg ccataacgtg tcgatgattt tccaggaacc
    18361 gcagtcgtgt cttgaccctt cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc
    18421 ctggacctat aaaggccgtt ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga
    18481 actgctgcac cgcgtgggga ttaaagatca caaagatgcg atgcgcagtt ttccctatga
    18541 gttgaccgaa ggtgaatgtc agaaagtgat gatagccatt gcactggcga atcaaccgcg
    18601 tctgctgatt gctgacgaac cgaccaactc aatggagcca acaacccagg cgcaaatctt
    18661 tcgcctgctg acgcgtctca accaaaacag caataccact attttgctta tcagccatga
    18721 cttacaaatg cttagccaat gggcggataa aattaacgtg ctttactgcg gtcaaacagt
    18781 ggaaaccgcg ccgagtaagg agttggtgac gatgccacat catccttata cccaggcgct
    18841 gatccgcgcg ataccagact tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct
    18901 gcccggcgct atcccactgc tggaacagtt accgattggt tgtcgtctgg ggccacgttg
    18961 cccgtatgca caacgagaat gcattgtgac gccacgtttg acgggggcga aaaatcatct
    19021 ctatgcctgt catttcccgc tgaacatgga gaaagagtga gatgatcgaa acgctgcttg
    19081 aagtgcgtaa tttaagtaaa acgttccgct accggaccgg ctggtttcgt cgtcagaccg
    19141 tagaagcggt aaaacccttg agctttacgc tacgtgaagg ccagacactg gcgattattg
    19201 gcgagaatgg ttcgggtaaa tccacgctgg caaaaatgct ggcgggaatg atagagccta
    19261 ccagcggcga gttattgatt gacgatcatc cactgcattt tggcgattat tccttccgca
    19321 gtcagcgtat tcgcatgatt tttcaggatc cttcgacctc gttgaatccc cgtcaacgta
    19381 tctcgcaaat tctggatttt ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc
    19441 gcaaacagat tattgaaacg atgcgtatgg ttgggctgct accggatcac gtcagttact
    19501 atccgcatat gttggcaccc ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat
    19561 tgcgcccaaa agtcattatt gccgatgaag ccctcgcttc actggatatg tcgatgcgtt
    19621 cgcagttgat taatctgatg ctggagttac aggaaaaaca gggcatttcg tatatttatg
    19681 ttacccagca tatcggaatg atgaagcaca ttagcgacca ggtgctggtg atgcatcagg
    19741 gcgaggttgt cgagcgaggc agcaccgcgg atgtgctggc atcaccgctg catgaactca
    19801 ccaaacggct gattgccggg cactttggcg aagcattgac agcggatgcg tggcgtaaag
    19861 atcgctaatt ttctgaatcc tccttccaga ttattcacct cattctgaca agagattaaa
    19921 tcttgtcaga atgaggtgat ggataaaata aaatcaaatg cgcgtgattt aagaagcaat
    19981 ctcactttgc aggaacgaaa gctctggcga tatcttcgca gccgacgttt tggtgatttc
    20041 aaatttcgcc gtcaacatcc ggtggggagc tacattctcg attttgcttg ctgctcggcg
    20101 cgtgtagtcg ttgagctgga tggtgggcag catgatttgg cagttgccta tgattccagg
    20161 cgcactagct ggcttgagtc gcagggctgg accgtgctgc gtttctggaa taacgagatt
    20221 gattgtaatg aggagacggt gctggagaat attctgcagg aactgaaccg ccggtcaccc
    20281 tctccctgaa agagcgaggg ggccgaccga gccgaatagc tgttgtggtg aaaatatggt
    20341 gacagttctg gagaatattc ggcaaggtct gaaccgtccc agccatcgcc ctgaaagggt
    20401 tagggggctg tataagcctg tttgttgctg gggtgacaat atttgcacaa tacggtcccc
    20461 tcgcccctct ggggagagga ttagggtgtg gggaaaagcg cccaccctgc ccgcaccctg
    20521 ctccggtcgg acctggcagc tatagccact cacagccagg ttgattataa taaccgttta
    20581 tctgttcgta ctgtttacta aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca
    20641 acaataagga ttaaagctat gggttttctt tccggtaagc gcattctggt aaccggtgtt
    20701 gccagcaaac tatccatcgc ctacggtatc gctcaggcga tgcaccgcga aggagctgaa
    20761 ctggcattca cctaccagaa cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa
    20821 ttgggttctg acatcgttct gcagtgcgat gttgcagaag atgccagcat cgacaccatg
    20881 ttcgctgaac tggggaaagt ttggccgaaa tttgacggtt tcgtacactc tattggtttt
    20941 gcacctggcg atcagctgga tggtgactat gttaacgccg ttacccgtga aggcttcaaa
    21001 attgcccacg acatcagctc ctacagcttc gttgcaatgg caaaagcttg ccgctccatg
    21061 ctgaatccgg gttctgccct gctgaccctt tcctaccttg gcgctgagcg cgctatcccg
    21121 aactacaacg ttatgggtct ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg
    21181 aacgcgatgg gtccggaagg tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact
    21241 ctggcggcct ccggtatcaa agacttccgc aaaatgctgg ctcattgcga agccgttacc
    21301 ccgattcgcc gtaccgttac tattgaagat gtgggtaact ccgcggcatt cctgtgctcc
    21361 gatctctctg ccggtatctc cggtgaagtg gtccacgttg acggcggttt cagcatcgct
    21421 gcaatgaacg aactcgaatt gaaataatca ttctgttggt aaatattggg cggcgttctg
    21481 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
    21541 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
    21601 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tgcggagtat
    21661 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg
    21721 atccttgatg aagaaacgct ggctcccttc ctttcatggc tggtccctgc gcgcgttctt
    21781 gcagtggaat tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat
    21841 gaacgcttgt ctacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
    21901 tactattcgg cgcgacttac gccgctcccc gggcctgatt cctccaggga aagcaatcat
    21961 aggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
    22021 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg ccggggactg tgtgatcatt
    22081 aaccaaatca ttggttttat tggctttcag gcgcggacca ttgcgacatt tcaggcttat
    22141 ctcgggcacc cggtacgctg gttacccggt ctggaaatac aaaactacgc cgacgcgtca
    22201 ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa
    22261 gagtacacaa aaagttcaac agcagaactt tgccaactgg ccgaaacact ctctctccac
    22321 cctatttcac tttcccttct cgaaaggttg ttaaacagta cacgggttaa tacacagccg
    22381 gataataagc ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc
    22441 gcctgtatgg attcatcaaa tgaatataaa aaaatcagcc cccttctgcg caagggcgaa
    22501 aatgaaatta accaatgggc tgaccgtcat tctgttgagc acgctaccgt tcaggcgata
    22561 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
    22621 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
    22681 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
    22741 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
    22801 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
    22861 ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt
    22921 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
    22981 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg
    23041 tttcgtgggt aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact
    23101 cttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg
    23161 cgactgggcg gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
    23221 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
    23281 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
    23341 aggtctggtt cgtgaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac
    23401 agaagatatg gatgacgccc ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat
    23461 tgtggcgatt gccgatccaa ccgcgtggat tgcagaaggc agcaagctgg acaaagccgc
    23521 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
    23581 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
    23641 catgacgctc tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat
    23701 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga
    23761 ctggcagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
    23821 acgccgcggt gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
    23881 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
    23941 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg
    24001 cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga
    24061 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
    24121 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
    24181 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt
    24241 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
    24301 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gcccacagga
    24361 tgaaatcact gtccaaatgg ccgagcgtcg ccgtctcaac cggatggcag aacgtgatgt
    24421 tggtgactgg ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc
    24481 agcggaaatt gtcgatatca gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc
    24541 tatcgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
    24601 ggaaaacggc actgtacaaa ttaaaggtga aacggtttac aaagtgactg acgttattga
    24661 cgtcactatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
    24721 atctcctttc acggcccatt cctcatggat gggccgttta tttccccgct ctaccttcgt
    24781 tatatccagg cagatttttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
    24841 gattacaaat aaatatataa aaaaaacaat gcgtttagcc gccgttaatt tcatgtaagt
    24901 catgttgttc cttttattta accgcggact ccgcagttaa ccggaggata tgcatcatga
    24961 aaaccgttag ggagtccaca acgttgtaca actttctcgg atcgcacaat ccatactggc
    25021 ggttgacgga aagcagcgat gttttgcgct tttctaccac cgaaaccaca gaacctgatc
    25081 gtactttgca gttatctgcc gaacaggctg ctcgcatcag ggaaatgacg gtcatcacct
    25141 ccagcctgat gatgagtctg accgtcgatg agagcgatct ttctgtgcat ctggtaggac
    25201 gaaaaatcaa taaacgggaa tgggctggca acgcgtctgc atggcatgac acaccggcgg
    25261 ttgctcgtga tttatcacac gggctttcct ttgctgagca ggtagtttct gaagcacatt
    25321 ccgcaatagt cattctcgac agccggggga atattcaacg cttcaatcgg ttatgtgaag
    25381 attacacagg gttgaaagaa cacgacgtca ttgggcaaag cgtgtttaaa ctgtttatga
    25441 gccgtcgtga agctgcggca tccaggcgca ataaccgtgt attttttcga agcggcaatg
    25501 catatgaagt cgaactgtgg ataccaacat gtaaaggcca gcggctgttt ctgtttcgca
    25561 ataaatttgt ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggcaccg
    25621 acattaccga agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta
    25681 tcaccggact gccgaatcgt aacgcaatgc aggatttaat cgatcacgct attaatcatg
    25741 cagataacaa taaagttggg gttgtgtatc ttgatttgga taatttcaaa aaggtcaacg
    25801 acgcctatgg gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa
    25861 gctgtctcga acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg
    25921 catccaacac ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac
    25981 ggctcccctt tcgcattggt ttaattgaag tttataccag ctgttcagta ggtattgcac
    26041 tctctcccga acatggttca gacagcacgg ctattattcg tcacgccgac acagcaatgt
    26101 acacagcgaa ggaaggcgga cgaggacaat tttgcgtttt taccccagaa atgaatcaac
    26161 gggtatttga atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt
    26221 tggttattca ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac
    26281 tagtacgttg gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg
    26341 ccgaagagtc agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc
    26401 aggtggcgaa gtggcgggat aaaggtatta acctgcgagt ggcggtaaat atttctgcac
    26461 gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt
    26521 ttgaatactg ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac
    26581 tggcactgtc tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt
    26641 ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac
    26701 ttgaccaggt ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg
    26761 cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg
    26821 caaaggaaga tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg
    26881 ccaaaccgat gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat
    26941 aagtagatga atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat
    27001 gataattagc cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt
    27061 ttccactttt gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga
    27121 acgtattgat attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa
    27181 tctcgagata cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc
    27241 gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg
    27301 gcgcgtcagt atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc
    27361 cgacaacaaa ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg
    27421 gcaaaagcca ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa
    27481 ctgagttacc tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa
    27541 acccgtatga tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg
    27601 gttcaaccgg gcgaaaccat ctttatcgaa aatggtagca gcaatgccct gctcgcccgg
    27661 actctgggcg aacagaagaa aaatgtcact atcatcacag tcagcagcta cattgcgcat
    27721 ttgctgaaag acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc
    27781 gaaagtatgg ttggcccttt gacacgccag tgcatccagc aggtacattt cagcaaagca
    27841 tttattggta ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt
    27901 accgatgtgg tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc
    27961 tcgaaatttg gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg
    28021 attaccgatt cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc
    28081 gttcacgtcg ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa
    28141 tcatccgctc taagatgatt cctggttgat aattaagact atttacctgt tattaacact
    28201 ctcaagatat aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat
    28261 aaccctgcgc gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat
    28321 agttatagag agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac
    28381 gagcaaaaaa atgaccgcgg ctgttctggc aattaccctg gcaatgtctc tgagtgcctg
    28441 ttctaactgg tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg
    28501 cggtgcagta ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg
    28561 tgttattggt caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat
    28621 tataatacgc ttgattctgt tcagtacgaa ataactgaat aatacacaca gtaaaatatt
    28681 cgaaagccac ggatatatcc gtggcttttt atgttaacca cctgcgagtt ttaccttcat
    28741 ccctttcgct tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac
    28801 tccatcttta actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt
    28861 cagttcggca tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc
    28921 actggtctga cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt
    28981 gggctcatca atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcac
    29041 catgcactcc gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat
    29101 tgcgttaccg ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc
    29161 gtcataatgc ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc
    29221 agtttgaact cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc
    29281 acaccatcaa ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt
    29341 gacagtgtca tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca
    29401 gcaatcaaaa gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc
    29461 atacgcgccc caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca
    29521 gcgacagcgt gcgcagcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca
    29581 aaaccacgct gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg
    29641 ccgaccttca gacgacaatc gcgtggttcg atcttgtcga caaaggacag cgcgtcatca
    29701 cgattatgat aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat
    29761 gaagcagtta acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac
    29821 aggtaaacgg cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc
    29881 tggcaggctt tttctgcgcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg
    29941 ttgtaactaa agtaggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta
    30001 atggttgacc aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca
    30061 catttctggc agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc
    30121 accatcaggc tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac
    30181 ttatggaaca cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg
    30241 gcctcactac cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg
    30301 gtgttctctt ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc
    30361 tgatagcagg tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc
    30421 acgcgttgca aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc
    30481 atcatgatgg atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc
    30541 atggcacgat cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg
    30601 gcaatttcga cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt
    30661 gctttctgcc actcactggt agcctggtag atttgtagca actgttgcag agcgccaatg
    30721 cggaagtcag tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg
    30781 gcggccatgt aatcacgccc tagttgttga atcgccaaca gacgctgttc ataggtcagc
    30841 gaggcgcttt ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa
    30901 cggaacaggt ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata
    30961 tcgagaaaca ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct
    31021 acgtaatcac gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg
    31081 cccatatacc agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata
    31141 gaaagttatt ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa
    31201 agctggtttt ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc
    31261 agccagaaca ggccacaaat caaccaaccg atagcaaacc ccgcagcaaa caataccgcc
    31321 agcaatgtgg aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga
    31381 tcgttctgcg cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg
    31441 agtaaatatt tcacattact tcccgttatg tggttagagc gaataaatta tggtcaactt
    31501 accgcaatag ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca
    31561 ttgttgatga tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg
    31621 ttgcgctatt tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc
    31681 aagccacgtt gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg
    31741 tggccaatgc atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa
    31801 gatagcaatg gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca
    31861 actggcagca aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg
    31921 tgaacgcaaa tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc
    31981 gcgttctgct cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag
    32041 ccagataaca aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg
    32101 tcccacaagg attgcggccg ccagaatggc aaataataca aaggcagcct taatgcgaaa
    32161 acgcagacac cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg
    32221 ggtgacagtt tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg
    32281 ccaacgccag ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac
    32341 tgcggtacgt ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata
    32401 actgaaaacg ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac
    32461 gcgtcgtgat taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca
    32521 ttatctggag aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg
    32581 gggcgatttc ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct
    32641 agtctatggc gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct
    32701 gaccggtgac gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt
    32761 gacgcaaatt gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa
    32821 cattggtctg ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt
    32881 agaggctaac caccagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga
    32941 tatgttcaaa ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt
    33001 cgaaattctg accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg
    33061 taaccccaat aacgaacatt atctcgatac caaagccgag aaaatgggcc atttgctgaa
    33121 caaataaccc tcttgcattg tgtaattagt ttgcttgccg gaagcaaaat aaccggcaag
    33181 caaatacttg ttacttcaac atattacgaa tgacataatg caaaatgccg tcgttctggt
    33241 agtaggtcaa ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac
    33301 catccgcgcg cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc
    33361 caatatcaat cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa
    33421 attccagcgg caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt
    33481 cggcaatcac cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg
    33541 agcctgatcc atactctttc ccggcaatca ccgccagcgg cgtttgttcc tgcttatagc
    33601 gcatcgcagc atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc
    33661 cttcaacgcc aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca
    33721 tcacttcatg gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc
    33781 gaccttgtag atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat
    33841 ggtcagtggt gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca
    33901 ctggtgctgg tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt
    33961 cctcctgcca accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc
    34021 cttcaaaaac ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac
    34081 gggcaatttc ttgtgccgat ggccagatat ctttcagata taccggatcg cctttacgat
    34141 catggccgat aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa
    34201 ccaccagcgg cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa
    34261 agttacggtt gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt
    34321 cgataggatc gggcagcggc ccagagttac caatacaggt ggtacaaccg tatcccacaa
    34381 ggttaaaccc cagttcgtcg agatacggtg tcaatttcgc ttttgccaga taatcagaaa
    34441 caactttcga gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagag
    34501 ttacggcttt tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg
    34561 tgcacgaggt tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt
    34621 tcataacata atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac
    34681 tggcggcaaa tgcttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc
    34741 ctgccaggct cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt
    34801 catcgcccgg gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct
    34861 gatcttcgct gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga
    34921 agccacaggt ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg
    34981 gtagtgaatc cagaccatca ccataaaatt cgacgaattt ccccaccacg ccatgtttgc
    35041 gcagcatttg ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac
    35101 cggtaagttt aaagcccact acgtccggga taagcatgga aaccggctgg cctaacattg
    35161 cggcttctgc ttcgatccca cccacgcccc accccagcac gccaaggccg ttgatcatag
    35221 tggtgtgcga gtcagtacca acgagtgtat ccggataagc aatccattca ccgtcctgca
    35281 attcactcca cactgctttg ccgagatatt cgaggttaac ctgatggcaa atgcctgtgc
    35341 ctggcggcac gacgctaaac cgactgaacg cttgctttcc ccatttcagg aacacataac
    35401 gttcgtggtt gcgctccatt tccaggcgta cgttttcttc aaatgcctca tcatcaccaa
    35461 aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc gggttaacct
    35521 ttgcagtatc gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga tcaacaacgg
    35581 caggtacgcc ggtaaagtcc tgcatcagca cccttgccgg gcggtaggca atttcacggt
    35641 cagcatgggc atttttcagc catcctgcca gcgcgtggat atcctcttcg gtaaccgagt
    35701 taccatcctg ccagcgcagc aggttttcga gcaaaacttt gagtgacttg ggcagacggg
    35761 tgatatcgcc cagtgattta gcagcaagcg gcaggctgta gtagtggtaa gttttatctt
    35821 tggcctgcaa cgtgtcctta ctggcttctc gtagggttga cgacatagct cctccttaat
    35881 gacagggttg cgaagccctg attctcttca gggtgaatat taaagataac acaaacatcg
    35941 cgtaacgatt tgataacaac ccaaattgat aaaagaggag aaaccttgtg gcaaagcaaa
    36001 tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag gagttcggcc
    36061 cagaagagca tcgacagaga aaacgcgccc atccaggacc aatatttcat atttgtgttg
    36121 atcataggct acacccttaa cggttattat tttgtgttga gataactcaa gctgttattg
    36181 ctgcggtgta ttaaaagccc tttaattcat taaaaatggg taataagaat gatgataatt
    36241 attgtttcgg ctgatcatct tcagcaaaca gttctataaa ggcttgctgc tgcgatgtta
    36301 gttgccagtc gtctggaata acgacggaac attcccgttt cgtttctgtt tcattgtcac
    36361 tatgctggca taatggtgac gactccagtt gctcctgacc gaccatagtt atccccacac
    36421 tttccaaatc agcagtgcga caaccaccca gaaaagtgca gaaccaagaa aaaccgccag
    36481 ccaggcttta cgttttaatt caggatcgcg gcggggttcc tgatttccag aaggcattgc
    36541 taacctcata taatcgccat cacttat
//
LOCUS       DAFAVF010000031        36501 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFAVF010000031 DAFAVF010000000
VERSION     DAFAVF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.031"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..235)
                     /locus_tag="LGK42_001512"
     CDS             complement(<1..235)
                     /locus_tag="LGK42_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657775.1"
                     /translation="MSAVNEIITNLASAGFLLFWRSIMQGEKQQPYFFNPGMTVEQLE
                     DWLEQQKLHLSRYNRLVKEKAELEERLSDISVEI"
     gene            complement(317..1069)
                     /locus_tag="LGK42_001513"
     CDS             complement(317..1069)
                     /locus_tag="LGK42_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBK2657776.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGMPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(1083..2072)
                     /locus_tag="LGK42_001514"
     CDS             complement(1083..2072)
                     /locus_tag="LGK42_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBK2657777.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNWARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2080..2877)
                     /locus_tag="LGK42_001515"
     CDS             complement(2080..2877)
                     /locus_tag="LGK42_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBK2657778.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2897..3286)
                     /locus_tag="LGK42_001516"
     CDS             complement(2897..3286)
                     /locus_tag="LGK42_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBK2657779.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPAAVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(3283..3609)
                     /locus_tag="LGK42_001517"
     CDS             complement(3283..3609)
                     /locus_tag="LGK42_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBK2657780.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3606..4259)
                     /locus_tag="LGK42_001518"
     CDS             complement(3606..4259)
                     /locus_tag="LGK42_001518"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBK2657781.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGVFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLEQVRQQAEKLVREMAA"
     gene            complement(4259..4753)
                     /locus_tag="LGK42_001519"
     CDS             complement(4259..4753)
                     /locus_tag="LGK42_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBK2657782.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4750..5691)
                     /locus_tag="LGK42_001520"
     CDS             complement(4750..5691)
                     /locus_tag="LGK42_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2657783.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQYFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAGFRG"
     gene            complement(5681..5860)
                     /locus_tag="LGK42_001521"
     CDS             complement(5681..5860)
                     /locus_tag="LGK42_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBK2657784.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(6036..6587)
                     /gene="ymfL"
                     /locus_tag="LGK42_001522"
     CDS             complement(6036..6587)
                     /gene="ymfL"
                     /locus_tag="LGK42_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HBK2657785.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(6631..6831)
                     /locus_tag="LGK42_001523"
     CDS             complement(6631..6831)
                     /locus_tag="LGK42_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2657786.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            6922..7596
                     /locus_tag="LGK42_001524"
     CDS             6922..7596
                     /locus_tag="LGK42_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HBK2657787.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            complement(7974..8441)
                     /locus_tag="LGK42_001525"
     CDS             complement(7974..8441)
                     /locus_tag="LGK42_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657788.1"
                     /translation="MTRDDLAFDIHYSFFLEKMNYTLLNRIDKGITLILIVLGFSVFA
                     KYSNMFIFGVTVAVLSVLQLVYQFGQEAGLSKEQMRQYKRLIVEFNTLSDDELRSRFL
                     KVQEADSNPWRSLQDAAFKRTCIALGRSDNSEISFIHSFIAWFAGDLPRNKKA"
     gene            8970..9332
                     /locus_tag="LGK42_001526"
     CDS             8970..9332
                     /locus_tag="LGK42_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657789.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            9398..10222
                     /locus_tag="LGK42_001527"
     CDS             9398..10222
                     /locus_tag="LGK42_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HBK2657790.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAIRKITIDANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            10350..10886
                     /gene="yfdR"
                     /locus_tag="LGK42_001528"
     CDS             10350..10886
                     /gene="yfdR"
                     /locus_tag="LGK42_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBK2657791.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSLWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            10877..11755
                     /locus_tag="LGK42_001529"
     CDS             10877..11755
                     /locus_tag="LGK42_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBK2657792.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQIRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLIPFTKYCDNHFGRGNYITWLGIRADEPRRLKPKSGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDIMYRGHLTLDGIARMSANSDYRNLYQAMVQARRFDTGSCSESC
                     EIWGDQLKLEFEEVGV"
     gene            11752..12096
                     /locus_tag="LGK42_001530"
     CDS             11752..12096
                     /locus_tag="LGK42_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK2657793.1"
                     /translation="MTTEINYHALLERARNKVQSIEFALTQSAFAEIRAELENDLELA
                     RIALASLEVEPDERAAYELFMEKRFGKTVDRRRAKNGDNEYMAWDMTLGWIVWQQRAG
                     IRFSTMSQQEVK"
     gene            12323..13486
                     /locus_tag="LGK42_001531"
     CDS             12323..13486
                     /locus_tag="LGK42_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2657794.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(13501..13576)
                     /locus_tag="LGK42_001532"
     tRNA            complement(13501..13576)
                     /locus_tag="LGK42_001532"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13541..13543),aa:Thr,seq:cgt)
     gene            complement(13691..14944)
                     /gene="proA"
                     /locus_tag="LGK42_001533"
     CDS             complement(13691..14944)
                     /gene="proA"
                     /locus_tag="LGK42_001533"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK2657795.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(14956..16059)
                     /gene="proB"
                     /locus_tag="LGK42_001534"
     CDS             complement(14956..16059)
                     /gene="proB"
                     /locus_tag="LGK42_001534"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK2657796.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            16347..17402
                     /gene="phoE"
                     /locus_tag="LGK42_001535"
     CDS             16347..17402
                     /gene="phoE"
                     /locus_tag="LGK42_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBK2657797.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            complement(17441..17842)
                     /gene="crl"
                     /locus_tag="LGK42_001536"
     CDS             complement(17441..17842)
                     /gene="crl"
                     /locus_tag="LGK42_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBK2657798.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(17900..19144)
                     /gene="frsA"
                     /locus_tag="LGK42_001537"
     CDS             complement(17900..19144)
                     /gene="frsA"
                     /locus_tag="LGK42_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBK2657799.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(19236..19694)
                     /gene="gpt"
                     /locus_tag="LGK42_001538"
     CDS             complement(19236..19694)
                     /gene="gpt"
                     /locus_tag="LGK42_001538"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK2657800.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            19955..21412
                     /gene="pepD"
                     /locus_tag="LGK42_001539"
     CDS             19955..21412
                     /gene="pepD"
                     /locus_tag="LGK42_001539"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBK2657801.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(21469..22083)
                     /locus_tag="LGK42_001540"
     CDS             complement(21469..22083)
                     /locus_tag="LGK42_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBK2657802.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(22080..23219)
                     /locus_tag="LGK42_001541"
     CDS             complement(22080..23219)
                     /locus_tag="LGK42_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBK2657803.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(23465..23917)
                     /locus_tag="LGK42_001542"
     CDS             complement(23465..23917)
                     /locus_tag="LGK42_001542"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2657804.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(23914..24969)
                     /gene="dinB"
                     /locus_tag="LGK42_001543"
     CDS             complement(23914..24969)
                     /gene="dinB"
                     /locus_tag="LGK42_001543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK2657805.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(25040..25825)
                     /gene="lafU"
                     /locus_tag="LGK42_001544"
     CDS             complement(25040..25825)
                     /gene="lafU"
                     /locus_tag="LGK42_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBK2657806.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTVLLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKRLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            25797..27509
                     /locus_tag="LGK42_001545"
                     /pseudo
     CDS             25797..27509
                     /locus_tag="LGK42_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            27614..27892
                     /locus_tag="LGK42_001546"
     CDS             27614..27892
                     /locus_tag="LGK42_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK2657807.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            27885..28241
                     /locus_tag="LGK42_001547"
     CDS             27885..28241
                     /locus_tag="LGK42_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK2657808.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAHM"
     gene            complement(28298..29071)
                     /locus_tag="LGK42_001548"
     CDS             complement(28298..29071)
                     /locus_tag="LGK42_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK2657809.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            29257..29517
                     /gene="dinJ"
                     /locus_tag="LGK42_001549"
     CDS             29257..29517
                     /gene="dinJ"
                     /locus_tag="LGK42_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBK2657810.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            29520..29798
                     /gene="yafQ"
                     /locus_tag="LGK42_001550"
     CDS             29520..29798
                     /gene="yafQ"
                     /locus_tag="LGK42_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBK2657811.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            29954..30694
                     /locus_tag="LGK42_001551"
     CDS             29954..30694
                     /locus_tag="LGK42_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBK2657812.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(30665..31432)
                     /locus_tag="LGK42_001552"
     CDS             complement(30665..31432)
                     /locus_tag="LGK42_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBK2657813.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(31638..32216)
                     /gene="lpcA"
                     /locus_tag="LGK42_001553"
     CDS             complement(31638..32216)
                     /gene="lpcA"
                     /locus_tag="LGK42_001553"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBK2657814.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            32456..34900
                     /gene="fadE"
                     /locus_tag="LGK42_001554"
     CDS             32456..34900
                     /gene="fadE"
                     /locus_tag="LGK42_001554"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBK2657815.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(34943..35389)
                     /gene="ivy"
                     /locus_tag="LGK42_001555"
     CDS             complement(34943..35389)
                     /gene="ivy"
                     /locus_tag="LGK42_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBK2657816.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            35570..36340
                     /gene="yafV"
                     /locus_tag="LGK42_001556"
     CDS             35570..36340
                     /gene="yafV"
                     /locus_tag="LGK42_001556"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBK2657817.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            complement(36382..>36501)
                     /locus_tag="LGK42_001557"
     CDS             complement(36382..>36501)
                     /locus_tag="LGK42_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBK2657818.1"
                     /translation="AINILTNDKVFKAGLRRKMRNAAMDRNYLASVLAESGLS"
BASE COUNT         9131 a         9367 c         9049 g         8954 t
ORIGIN      
        1 caatttccac agaaatatca ctgagccgtt cttcaagctc tgctttttct tttaccagac
       61 ggttatagcg gcttagatga agcttttgct gctccagcca gtcttcaagc tgttcaacag
      121 tcataccagg gttaaaaaaa tatggctgct gcttttcgcc ctgcattatt gacctccaga
      181 aaagcaaaaa ccccgccgaa gcgaggtttg ttatgatttc gttaacggca gacatacaaa
      241 gcccatcgtt aggaaaatcc taaccagatt ttttgaaaaa tgcaatgatc atgtcgccat
      301 cttcggtaaa aatcatttat cttgtcactt ttctcaattg tgtctcagca tatgcttctt
      361 cctgccagca ctttgtaacc agtttatcaa tgacatctgc atatcctttg taccactgat
      421 aatccgtcag gtctggtacc agcttctgga catgatgccg cgccagtgtg gttggtaaac
      481 ggctaaaccg gtttccattg caacgcccac aaatcttata aacaggcatg ccatgaagcc
      541 gggttctttt ttcatccagg acaatacctt tacccttaca ccctctgcac gctgtgctga
      601 cttctccctt accatgacaa tgctgacata gttccttcac ccactcctcc ttgataacag
      661 attcaccgct tctggagtgt ttcaccactt cgcgcaatac attatgaaat ccagtaccag
      721 cacaatgctc acaacgagcc ttacttgccg cagacctgga ataatcagca aaggcaaaat
      781 tcacaaggta aggaataatc tgtagccggg tttcttcact caatttattc aatgtcgggt
      841 tatccagtgc catcgcgtaa ttgagcagac cttcaatcgc aaactgagga tcctgaacac
      901 caacttttgc caggaataag gcaaacccaa gcggtgcttt cgactgcacc atcccctgcg
      961 cagccatcac atccgtaatt gttaaaccac cagagcctgt cgccggtgcg tcatcgctca
     1021 gttttggaga tttcggggag taatatttcg gtaaagcttc aaggttcatg ctcgttctcc
     1081 acttacgcca gtacgcctat tgccagcgca cgatcgataa aacgaaatat cagctccagc
     1141 tgggagccat acttctcttc aaatgccacg gtatccgcat gcagctcgtc gtgatgcttt
     1201 ctgcacaaag gcaacacaaa gaggtcatgc gcttttgtac ccattccccc ctgaccgtga
     1261 cctatcaggt ggtggggatc atcagcgggc tttccacaac atgcacacgg ctgtgtctta
     1321 acccagcgcg tgtacttttc attaacccag cggcgacgtt ttgggcgtaa cataaaagac
     1381 tccggcgact ccggatccac tttcagcgcc agcacctttt tcgccttatc ctggatgatg
     1441 ctggtggcag gaaccgaagg cacaaggtca ctttcccggg tgacagacgg cacaacaggc
     1501 tttggtaatc tcagtgcctt acgggctgca ctttccggta aggcatccgc caggtcatta
     1561 cgaatcagcc accagcacag ttccggcatt gtcacaacgt gactatcatc aaaaccgaga
     1621 tcccgacgca caacagacaa cacccagcgg gcccagttat ccgttgccat tgattccagc
     1681 cgttccgtga actgatcgcg cagctggtta tcacagtgcc agcacagacg gattgcgccc
     1741 ggagcgtgtc gcattgttgt catgttctcg ctgtgccagt cggaatgagg ccactggcag
     1801 cctttttcac gaagtaacca gctttcaaga cattccacgc caccagcacg acggatcact
     1861 gcctcattgc ggaacacggc ccgaacagca ggatcatccg ccagcggttg tgatgccgcg
     1921 ggaacggcac cactggcgaa agatgaataa cgttccggct caggctccag cagtacacgc
     1981 ccctgcataa acaggggcat cagctctgaa cctggtctga acaatacgat ccccatacgc
     2041 ggggcaattt caggagtcag tagtgctctc acggtcacct caatgaacgg tatcgagcag
     2101 ctttaacagc tcagggaatc gggattcgaa gaaatgcggc tgcgtctcgc gcggatttgc
     2161 gggactggtg atgttcttgc cgaacatgca gcctttcgct gtcagcgacc agaatttttt
     2221 gatgttgtta atcgcggtac ggctgtatcg ttcgcgttgt tcaacgatcc ccagcttcgc
     2281 catctggtga tatgcctgat tagccgtcag gcggatacca tactgcttca gcagtgcact
     2341 cagcgacagc gtcgggcggc ttgagccatc aggcgcgtca gcaggtgcat caatggcata
     2401 gcgcggtgcc agattcggta agccaacagc ctcctggagt ttctgacagg caccaagcac
     2461 tgaagagtta gacagattta attcccggcg cataaagtcc agcagaatca caccagcctg
     2521 catcttgtca gcagcctgtc cggataattt ttccggtgcg ctggttacca tgtcgaaagt
     2581 acggatcacc ttaagatgga atgacgggct tatccacatt gcataggcat acaccagttc
     2641 tttgcagaca tacgtcccct ggttatttcc gccacgaata acgttaactg gctctatatt
     2701 gaccgagttg caaatctgca actcgcttat taaacgttca gtttgctcat tgcggagcca
     2761 gaatgcaggc ttatgcttat ccagagaacc ggcagccctg tgaagatcgt tcaggctgta
     2821 acgaccataa gcatcacgac gaacttcaat accatcaatg accatcagat tattcatact
     2881 tcgtttctcc tcttgatcag gcggctgcac ccgccgtttt ctcgtactta ctgatagtga
     2941 tctcgacctt cccttccggg ataaccggtc cccactccac cagcattctt ttcacctgac
     3001 tgtcgtcttc ccacaccccc gcgtgggtca gggcgtcaaa cagcgccttg ttatagttgt
     3061 ccagatcgcg gatccggtta tccggtggaa acaacacgat ctccactgca gcaggtgccg
     3121 acgttggttt cggcagacga cgtaactgct caactattgc tgcgcacgcc gcgctctgga
     3181 attttcgccc cgccgcgctt atcaggctct taccagcaaa cgcccctttg ttggggtgtc
     3241 gccagtacgt gttcacgctg ggcggaaaag gcagaatcag cttcatactt tcaggcccct
     3301 ctcatgtaac cagtgggctg cacgcagcct ggcgttttcc tcaccggcaa gcagtgagcg
     3361 gataatcccg accgcctcgc tgtcgtcgtc cttcaccgcg gtatgaagcg ttatcccccg
     3421 ggccacgcca cgctttatcg tgatgacgcc ttttttctcc agcgcacgaa gatgctccac
     3481 cgctgcattc accgaacggt atcccagcat ggttgccacc tcctgattgg ttggcgggaa
     3541 accacgttct ttctgataag aaatcagcat atccagcacc tgctgctggc attgagttaa
     3601 cgtcgtcatg ccgccatctc cctgaccagt ttttccgcct gctgacgaac ctgctccaga
     3661 aacgcctcac cacatgcctc aagttcatcg cgcccgatgt agctgattgc cggtcccttc
     3721 caggtcttgt cgaaaacagc aatagcacca gcgaagaaaa cgcctgtcgg cacctgcttc
     3781 tcatccttcg ggataaacca ggcaggcagt tcaaaaccaa tacgcccgcg aataaaagca
     3841 atatgatctg catcttccgg ccaccacact tcgctggtgg cagctttgat caggaaaaca
     3901 tagcgcccgc ccttatcacg catggcactg gcatgtttca tgatgtaacg catgccggtg
     3961 atgtattgcc cctcatgctg actggcgcgg ctgtatgggg gattaccaaa ggcagcacct
     4021 ttaagctccg caagacgttc tgaccagtca tgcgccagcg cgttgtcttc cgccgtgtaa
     4081 tacgcagcac atttggcgtt atcaccgtca gtgaacagat ccagaacaaa cgggccaaac
     4141 agggtgttaa ttccccagaa aatgttgtcc ggcgtgcgcc actgatcgcc cacttccttc
     4201 agttcatggg ctggtttgtt ccgcagttcc accagcgcct ggcaatattt attactcatt
     4261 aagcccccac gtaattccct gacagatacc actcttcacc cgatgcagcg cgcttgctgc
     4321 ttttccgtaa gcaccgctca cgacgcgcca gaaaattgtt tcgttctggc tgggagtggc
     4381 tttcacggaa tgccgccatc cacacggttg cagcacgacg gtataagccc cttgactcca
     4441 gttcttcagc ctggcgggtc aggcacaaaa tcacccgtgg atcgttagtg ccgacataga
     4501 aattgcgcac aggtctggtt tcacgaactg gttgtggttc cggctcctgc gctctctcag
     4561 tcaggcgcgg gaaatgtctg cgtgtatccc cttcacaacg gtgagccaca cgcccactct
     4621 gacgtaactt gcttgctgac tgcagaacgc gctgccgtga gtaaccagca aaagcatccg
     4681 caatgtctcc ggaagtacac cccggatggg cttcaatgaa tttctgaacg tcatttaaca
     4741 gactcatgat caccccctga atcctgccgg gatctggctg tagtccacgt tgtcgtaact
     4801 ggatttgaag tacgggtctt cgcgtttttc ggtgtacgtg cttacggacg gcgataagcg
     4861 cagggaaagc tcatcccatt tttcccgcag cttcgacggg ctgagcacgt tacggcacca
     4921 gaacggatcg cggctgacgc ggctgtacat ctcgcagatt tgtttgtgag tacgaccatc
     4981 ctgcacacac atcaggcgaa tttcgtttgc ccaggctgtc cagttcggtt ctttgggacg
     5041 aaccacctcg ccgtcacatt cggcagcctg ctcgtacagg gcgatgattt ttttccagag
     5101 ccactgtgcg caggtcaaat catcctgcgt cccccactgg cgctttttag ggctgaatac
     5161 aaccgcatca ggatggcgag ttaaaaaata ctgttcagcc gtctgcgtgt ccggttgcga
     5221 agcatccgga cgagaaggtt ttttatctga cggatcatgt tttgatttta ctgacggatc
     5281 cccgccagat tctgacgggt gaaaacccgc ttttttgcca gatttcgacg catcaaattt
     5341 tgacgggtca gattttgatg cgtcagattt tgacgggtca gaatctgaca gttgagaaaa
     5401 tgccgctgcc tgaagcttcg caacgttaag ctgataaaca ttcgacgcat tgcggttacc
     5461 ctggcgacga gccttacgcg ttaaccagcc ttctgcttcc agccgtgcga tagccgttct
     5521 gacggtactc atccccgcgc caatctgacg agcaatggtt tcaattgatg gccagcacac
     5581 accttcgtca ttactgaaat cagccaggcg ggccataatt gccacgctgg ataacttcat
     5641 gcctgatgca gcgcaaccat cccatacata gccggttaat ttagtgctca tgaccgacct
     5701 ctatttccct gaatttacga cgaaactgtt cgagcgggct gaagcactca tgctcatagc
     5761 cttcacggag gtagataacc cgttgtgttt ccggctccca acgaatgact ctgacgggca
     5821 ctccgtagtg atctttgaac cagcggttaa cttgtcgcaa aggactgtct ccttctgccg
     5881 gttgaaatca cccacagccc actctgcaaa gctgtgggtt acaatttccc tgtcacctgg
     5941 tacatttact gcatagcaat actccacctt cgcttttcca cctggtacag gaagtgcaat
     6001 cagttgcgag caacggtagt gtgttgttaa actgttcatg cgttagtttc tccacagtca
     6061 cgacacgcca cggcgcccgg agctgcacac tcgcgggcgt cactactttc tgaaacgcaa
     6121 aagattttgt agaccagtgc tgcatgctcc tgcagcttcg aaattgagag gtacagctca
     6181 tcgttaattg ctgtcttctc atgcggttcc actacaccgt cttcaattgc tgaacgaatc
     6241 tgttttgaat aactgccgat ctgttcaatg acttccagca ggcgttggtt gatatcggcg
     6301 ttgtccacat cctcgacgtc aggaagagac acaaagacgc catttgcaga ctgcgccaca
     6361 gcatcagcaa tgaagtgagt gccaccagca cgctgtaaaa ccattgccca tcccagcggg
     6421 aaaatctgat cgccatctgc acgaaggcgg ttgaataaag cgttttctgt tacatcgagc
     6481 cagtcagccg cttcagcgta accacccggc aacgccgcga tagtttttct gacagctttc
     6541 acgtaccact caggctgttt ttctattttc cagtgatgct tacccacgat tagcctcatc
     6601 gttctgtggt taaaaattga aagtgttctg ctaatctttc ggatagatat ccggtcttaa
     6661 gtcagatttc gtaattgcac ctgacgtgca ttgctcaagt tttttagcca gcacaaaact
     6721 ggctttttta tagccattga aaaccagccg taagtagcca ggtgttgagc caacttttcc
     6781 ggccaactcg ccctgctgtt ctttggttaa agagtcccaa tacgctttca tacaatatgt
     6841 acctccggtg tacatattac atgattgaaa tgaaccttca agatacttgt accttaacgg
     6901 tacaagggtt ttaatttcgt tatgaaaaca atccatgaca tccggcggtc taacgccaga
     6961 aaactgagag atggtgttgg cgggaattct tcctttgcca ctatgattga tcgcgagcca
     7021 acccagacca gcaggtttat gggagatggt gctactaaaa atatcggtga cagcatggca
     7081 cgacacatcg aaaaatgttt cgacctgcct gtcggatggc tcgatcaaga acaccagaca
     7141 acgaacatca caaaaaaacc tgatgtttca atcactaata aacaaatcac attagtccct
     7201 gtcatatcat gggtacaggc cggagcatgg aaagaagttg gatattctga ggttgatttg
     7261 agcacagcag aaacgtatcc ctgccctgta ccctgtgggg aaatgactta tatcttgcgg
     7321 gtgataggtg attcaatgat tgatgagtac cgcccgggag acatgatttt tgtcgatcct
     7381 gaagtacctg cctgccacgg tgacgacgtt attgcattga tgcacgatac aggtgaaacc
     7441 accttcaaaa ggttgataga agatgggaca cagcgttatc tcaaagcgtt aaacccaaac
     7501 tggcctgagc cttacattaa gatcaacggt aattgctcta taattggtac tgtgattttc
     7561 tcaggaaaac caagaagata caaaatcaaa gcctaatcaa tgtttatgaa cctgcttcgg
     7621 caggtttttt tatacttgac aatgtacctt tgagatacat aatgtaccca agaaaaacaa
     7681 caaacaggca ggacgcccac gaagtagccg cctggggcat atgaagtcca ggatgattcg
     7741 ttagcaacaa aaaagcgccc tttcggacgc ttcgctcttt aacaatctgg attgctaaca
     7801 acaattactc tttaggtttt ggttgaggca ctggccgtgg ataaagagga acatgttcaa
     7861 acataggctc atcctgagag tcgctgaaca tttcaagcct actttttggc aacggcggtc
     7921 tcggcttagg aggaacgtga ttaggtatga taggtattgg ttttgacata aattcatgcc
     7981 tttttatttc gtggaagatc accggcgaac catgcaataa aactatgtat gaatgatatt
     8041 tctgaattat cactccgacc aagggcgata caagtccgct taaaagcggc atcttgtaga
     8101 gatctccaag ggttactgtc agcctcctgt accttcaaaa acctagaacg caactcgtca
     8161 tcagaaagag tattaaactc aacaatcaat cgcttatatt gcctcatttg ctcttttgat
     8221 aacccagctt cttgtccaaa ctgataaact agttgaagta cagacaaaac tgcgacagtg
     8281 acgccaaaga taaacatatt actgtattta gcaaaaacag aaaaacccaa gacaataagg
     8341 ataagcgtaa ttcccttatc tattcgattc aaaagagtgt aattcatttt ctcaagaaaa
     8401 aatgagtaat gaatatcgaa tgctagatca tctcgagtca tgttactccc ttacaattca
     8461 ggttttggct gaggggtcgg agctggccgc tgatttaccg gaacatgata tttttctttg
     8521 ttatcaacac acatttttct ttcctaattg ttgttggaaa atccagatta taccgaattc
     8581 ttgttattgg ggaataacca agtccacctc acctgatgtg gctaaaagca ggcacataac
     8641 agctaagtat tttcaaccag agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac
     8701 gcgggttaag gttgaggctg acagtcgacc ttctgtggat acccatacgc ctgagtgaca
     8761 acccagccac aacagccact gctttggcgg taccagtttg tacacttgct tccggctggt
     8821 accgctcttt ttacaaaaca gagaagagca tcaccggacg acgggctcat aacccaatcc
     8881 atccgggcgg ctgccaccgc aggtgttctt ctctgttttg tggagaaacc aaccgacctt
     8941 gcagggtcga tatgatgagg agcagcaaaa tggctagcga acgcagtact gatgtgcagg
     9001 catttatcgg ggagctggac ggcggcgtat ttgaaacaaa aatcggcgca gttctcagtg
     9061 aagtcgcttc cggtgtgatg aacacgaaaa ccaaagggaa ggtctcactc aacctggaaa
     9121 tcgaaccatt tgatgagaac cgtgtgaaaa tcaaacacaa actctcatat gttcgcccga
     9181 ctaaccgcgg gaaaatttcc gaagaagaca ccaccgaaac gccgatgtat gttaatcgcg
     9241 gtggtcgcct gactattctg caggaagacc agggacaatt actgactctt gccggtgaac
     9301 ctgacggaaa actccgcgca gcaggtcatt aatatcgtta ttaataaact gattatttat
     9361 ctcatcactg aatatcttta tatagtgagg acttattatg tctcagaact tagacgcaac
     9421 cgcaattaat caaatccatg cccttatttc tgctcagggt gttaatgaaa ttatcagtaa
     9481 gattggtgcc gatgctgtgg cattgcctga aaatttccgc attcatgatc tggaaaaatt
     9541 taatttaaat cgcttccgtt tccgtggtgc gctttccact gccagcatcg atgactttac
     9601 ccgttattct aaagatcttg cagatgaagg cacccgctgc tttatcgatg ctgataatat
     9661 gcgtgccgtc agtgtgctta acctgggtac tattgatgaa ccaggtcacg cagataacac
     9721 cgccactctc aaactgaaaa agacagcacc gttctctgcc ctgttgtctg ttaacggcga
     9781 gcgtaactcc cagaaatcac tggcagaatg gattgaagac tgggccgact accttgtggg
     9841 ctttgatgct aatggtgacg ccattcaggc aacaaaagcg gctgcggcaa tccgtaaaat
     9901 cacgattgat gcaaaccaga ccgctgattt tgaagataat gacttcagcg gcaaacgctc
     9961 cctgatggaa tctgtcgaag cgaagaccaa agacattatg ccagtggcat ttgaatttaa
    10021 atgcgttccg tttgaaggtc tgaaagaacg tccgtttaaa ttacgcctca gcattatcac
    10081 tggcgatcgt cctgtactgg ttctgcgcat tattcagctg gaagcggtgc aggaagatat
    10141 ggctaacgaa tttcgtgatc tgcttgttga gaaattcaaa gacagcaaag tagaaacctt
    10201 tataggtact ttcaccgcct gatttcatta ctgcaaatgc ccctgcgggg gcatttatgg
    10261 aaacgtaatt aactcaataa tcaccggatg gtgagggctt ccttttaccc aaactcagcg
    10321 cggtgcagcg catatacgtg gagaacaaaa tgtcatttat taaaactttt tccgggaagc
    10381 atttttatta tgacaagata aataaagacg acatcgtgat taacgatatc gcggtttccc
    10441 tttcaaatat ctgtcgcttt gccggtcatc tttctcactt ctacagtgtc gcccaacatg
    10501 cggtgctttg cagccagctg gtgccgcagg aatttgcttt tgaagcgtta atgcatgatg
    10561 caacagaagc gtattgccag gacatccccg caccactgaa acgccttctt cctgactata
    10621 aacggatgga agaaaaaata gatgcagtaa tccgtgagaa atacgggtta cctcctgtta
    10681 tgagcacgcc agtgaaatat gccgatctca ttatgctggc aaccgaacgc cgtgatctcg
    10741 ggcttgatga tggctcttta tggcctgtac tggaaggtat cccggcaaca gagatgttca
    10801 aagttattcc actggcaccg ggccatgcct acgggatgtt tatggaacgc ttcaacgagt
    10861 tatcggaatt acgcaaatgt gcataactca tgtagttagt ttttctggcg ggagaacatc
    10921 cgcatatctt gttcacctga tggaagaaca aagaaaggct ggcaataacg tctgttacat
    10981 ctttatggat accggttgcg aacatccgct gacataccgc tttatccggg aggttgtaaa
    11041 gttctgggac ataccactaa ctgtgttaca ggtcgatata aatcctgagc ttgggcagcc
    11101 aaatggttat acagaatggg agccaaagga tattcagata cgaatgccgg tgcttaaacc
    11161 gtttatggac atggttaaaa agtacggcac gccatacatc ggcggcgcgt tctgtactga
    11221 caggctaaaa ctcatccctt tcacgaaata ctgcgataac catttcgggc gaggtaatta
    11281 catcacatgg ctgggtattc gtgcagacga accccgtagg ctgaaaccga aatcgggcgt
    11341 ccggtatctt gccgagctgt cagattttga taagtcggat gttatccggt ggtggcgaaa
    11401 acaacctttt gatttgcaaa tcccggagca tctcgggaac tgtgttttct gcatcaaaaa
    11461 gtcaacgcaa aagctggggc ttgcatgtaa agacgaacca ggtctgatgc gagtttttaa
    11521 tgagctggtt acaggcaaac acgtcaggga tggtcatcgc agaacaggta aagacattat
    11581 gtaccgtggt cacctgacgc ttgacggaat tgccagaatg tctgccaaca gcgactacag
    11641 aaatttgtat caggcgatgg tacaggccag gcgattcgat accggttcgt gttcagagtc
    11701 atgtgaaatc tggggtgatc aattgaaatt ggaattcgaa gaggtagggg tatgacaacc
    11761 gaaattaact accatgcact gcttgagcgc gcacggaata aagtgcagag cattgagttc
    11821 gccttaacac agagtgcatt cgctgagatt cgcgctgagc ttgaaaatga tttagaactg
    11881 gcacggattg cactggcatc tctggaagtt gagccagatg aacgcgcagc ctatgaatta
    11941 tttatggaaa agcgtttcgg taaaacagtc gatcgtcgga gagcaaaaaa cggcgataac
    12001 gaatacatgg catgggatat gactctcggt tggatcgtct ggcagcaacg agctggtatc
    12061 cgtttttcaa caatgtcaca gcaagaggtg aaataatgga gccatacagc ctcacactcg
    12121 atgaggcctg tcattttctc aagatatcca gaccgactgc tattaactgg atacgcacag
    12181 ggcgtcttca ggcaacacgc aaagatccca ctaagaataa atctccttac ctcacaacac
    12241 gacaagcctg cattgcggct cttcagtctc cgctgcatac tgtccaggtg agcgcgggtg
    12301 atggcataac agaggaaaga aaatgtcact cttccgcaga ggtgaaatat ggtacgccag
    12361 tttcacattg ccgaacggta aaagatttaa acagtctctt ggaacaaagg acaaaaggca
    12421 ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg cgggtcagta aacttggtga
    12481 aatacctgat ataacgttcg aggaagcatg tgtcaggtgg cttgaagaga aagcacataa
    12541 aaaatcactg gacgatgaca aaagccggat cggattctgg cttcaacatt tcgcaggaat
    12601 gcaactaaga gacattactg aatcaaaaat ttattcagca atgcagaaaa tgacgaaccg
    12661 gcgtcatgag gaaaactgga aactcagggc agaagcatgc agaaaaaaag ggaaacctgt
    12721 tccagaatac acgccaaaac cagcgtccgt tgcaacgaag gctacgcatc tttcatttat
    12781 aaaggcccta ctaagagccg cagagcgtga atggaaaatg ctggataagg caccaattat
    12841 taaagtgcct caaccaaaga ataaacggat ccgctggctg gagccccatg aagcacaaag
    12901 gctgattgat gaatgtccgg agccattaaa gtctgttgtt gaatttgcac tggcaacagg
    12961 cttaagacgc tcgaacatca tcaaccttga atggcaacaa atagatatgc agcgccgggt
    13021 ggcatggata aacccggaag agagtaaatc aaaccgcgca atcggcgttg cgctgaatga
    13081 tactgcatgt cgcgttttga aaaaacaaat cgggaatcat caccgttggg tatttgtgta
    13141 caaggaaagc tgtaccaaac cagacggaac gaaagcgcca acagtaagga agatgcggta
    13201 tgacgcaaac acagcctgga aagcggcgct gagacgggct ggtattgatg atttcagatt
    13261 tcacgacttg agacacacct gggcaagttg gctggttcaa gccggagtcc cgttgtcagt
    13321 gttacaggaa atgggaggct gggagtctat cgaaatggtt cgtcgatatg ctcaccttgc
    13381 acctaatcac cttaccgaac acgcacggca aatagactcg atcctgaacc catcggtccc
    13441 aaatttgtcc cagtcaaaaa ataaggaagg tactaatgat gtgtaactta ttgatttaaa
    13501 tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaatgcgc tgctctacca
    13561 actgagctat atcggccctg aaaggacatg ttcacgaacg tgaatcacgg tggacaaggt
    13621 taaaactaac cgggcgatgc gtcaatggcc ttgtgaatca aatggctact tttgcatcac
    13681 ccggttttat ttacgcacga atggtgtaat caccaatgcc gatccacttg taagtggtca
    13741 gtgcttccag ccccattggg ccacgcgcgt ggagtttttg tgtgcttacc gccacttccg
    13801 cacccagacc aaactggccg ccgtcggtaa aacgcgtaga ggcgttaacg taaacagcgg
    13861 acgaatccac ttcgttaaca aaacgctggg cgttgcgcat atcgcgggtc aggatcgcat
    13921 cggagtgttg tgtgccgtgt tcacgaatat gggcgatggc atcgtcaaga tcgctgacga
    13981 ttttgacgtt caaatctaat gacagaaact catcgtcata ctcttcggct ttaacagcaa
    14041 ccaccttcgc agggcctgcc tgcaactgcg ccagtgcagc tgcatctgcg tgtaatgtca
    14101 cgccgctttc cgccatttgt ttgcttaatg cgggcaggaa gctatcggcg atgtttttat
    14161 tcaccagcaa cgtttcaacc gtattacatg tgctcggacg ctgagttttc gcgttgacga
    14221 tcacttttaa tgcttcagcg atctctacac tttcatcaac gtaaatatgg catacgccta
    14281 taccacctgt gatcaccggg attgtcgact gttcacggca cagtttatgc aaaccagcgc
    14341 caccacgcgg gatcagcatg tcgatgtatt tatccatacg cagcatttca ctgaccagcg
    14401 cacggtcagg attatcaatc gcctgcacgg cacccgccgg taagccgcag gatttcaggg
    14461 cgtcctgaat caccgccacc gttgcagcgt tagtgcgaca cgtttctttg ccaccgcgca
    14521 ggatcaccgc attaccggtt ttcaggcaca gcgaagcgac atcaaccgtc acgttcgggc
    14581 gcgcttcata aatcacgcca ataaccccca gcggtacgcg acgacgctca agacgcaggc
    14641 cgctgtccag tacgctgcca tcgattacct gccccaccgg atcggcgagg ttacacacct
    14701 ggcgcacatc atcggcaatg cctttcagcc gtgcgggcgt cagtgccaga cggtcaagca
    14761 tcgcttcgcc aaggccattg gcacgcgcgt cagcaacatc ctgggcgtta gcgttgagga
    14821 tgatttcgct ttgtgcttcc agttcatcgg cgattttttc cagcacgcga tttttttcgc
    14881 ggctggagag ttgcgctaat ttatacgagg cttgcttcgc ggcaatgccc atttgttcca
    14941 gcatcagcct gctccttaac gggtaatcat gtcatcacgg tgaacggcaa ccgggccgta
    15001 ttcatatccc agtattgcat caatttcttg cgagtggtgc ccggcaatac ggcgtaatgc
    15061 atcgctgttg taacgactga cgccgtgggc gatatcgcga ccttcgaggt tgcaaatgcg
    15121 gatgacttca ccacgcgaga aattgccagt cacgctttta atgcctttcg gcaacaggga
    15181 gctgccgcgt tcaagaatgg cggcagttgc cccttcatct accgtgattt cacccgccgg
    15241 cggcgcaccg aaaatccagc gtttacggtt ttcaagcgga gtcgcctggg catggaacag
    15301 cgtaccgacg gaaatgcctt ccatcacatc accaataacg cccggcttgc tgcccgcggc
    15361 aataatggtg tcgatacccg cacggcaagc cacgtcagcg gcctgcaatt tggtactcat
    15421 gccgccagtt ccgaggcctg aaacgctgtc accggcaatc gcgcgcagtg cgtcatcaat
    15481 gccgtaaaca tctttaatca gttctgcctg cggattgctg cgcggatcag cggtatacaa
    15541 acctttttga tcggtcagca gcaacagttt atcggcaccc gccagaatcg ccgccagcgc
    15601 agaaaggtta tcgttatcgc cgaccttaat ctctgccgta gcgacagcat cgttctcatt
    15661 gattaccgga acgatattgt tatcgagcaa cgcacgcagg gtgtcgcggg cgttcaggaa
    15721 gcgttcacgg tcttccatat cagcacgggt cagcagcatc tgcccgacgt gaatgccata
    15781 aatcgaaaac agctgttccc acagttgaat cagtcgactc tgccctaccg ccgccagcag
    15841 ttgtttcgag gcgatagtcg ctggcagttc cgggtacccc aggtgctcac gtccggcggc
    15901 gatcgcgccc gacgtcacaa taacaatccg atgcccggcg gcatgtaact gcgcgcactg
    15961 gcgaacaagt tcaacgatat gggcacggtt cagacggcgc gatccgcctg ttagcacact
    16021 ggtgccgagt tttaccacca gcgtctggct gtcactcatg attctctgcc attcaatttt
    16081 aggaaaaatg atatcaaacg aacgttttag caggactgtc gtcggttgcc aaccatctgc
    16141 aagcaaagca tggcgttttg ttgcgcggga tcagcaagcc tagcggcagt tgtttacgct
    16201 tttattacag atttaataaa ttaccacatt ttaagaatat tattaatctg taatatatct
    16261 ttaacaatct caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg
    16321 cgcgttaatt aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg
    16381 gcattgtggc atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac
    16441 tggatgtcta tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg
    16501 gcgaccagag ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga
    16561 ctggctatgg ccgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac
    16621 agcaaaaaac gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact
    16681 atggccgtaa cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat
    16741 ttggtggcga ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg
    16801 cgacgtatcg gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat
    16861 atcaagggaa aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt
    16921 cattgacata tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag
    16981 atcgcaccaa cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg
    17041 ctacaggtct gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac
    17101 gcaaaatgac gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg
    17161 ctcaatacca gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga
    17221 aagatattga aggtatcggt ggtgaagatc tggttaatta tatcgacgtc ggtgctacgt
    17281 attatttcaa caaaaatatg tcagcatttg ttgattataa aatcaaccaa ctggatagcg
    17341 ataacaaatt gaatattaat aatgatgata ttgtcgcgat tgggatgacg tatcagtttt
    17401 aatagatatt gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg
    17461 gctcgtcacg aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac
    17521 gcagcttctc gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtatctt
    17581 ttaccggaac actcttccag tcgcctgctt tatcgaacag accaaactgg tagctgtagg
    17641 taaaacggga ttcctgcgct tccagctcca tccaccagcc ccagaattca cgcacttccg
    17701 gtgccggttt cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc
    17761 acttaccttc acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg
    17821 ggtgtccact cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt
    17881 ggcaaaattt agcaaatttt taacacaagc gtttttcgat ccagccggtg atttcctgaa
    17941 gacctttgtc aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag
    18001 cagatgatga ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt
    18061 agcctgataa cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat
    18121 agcgattcag ctccacgcgt aacgcttcgt cggaagcatc atgcatcccc aaacgactcg
    18181 ccagaacgtc aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg
    18241 tatgaactac cggaccaaga caggcaaccg ctttcagacg cggcgattca aggtatgcca
    18301 gacgcacggc aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca
    18361 cccacggtac gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg
    18421 tgagcttcca ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc
    18481 cgcgcggcgc aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac
    18541 caccacacat taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg
    18601 tgatgggcgc accgccgggt acagtaaact ccatctgccg catcgtgccc ggcagacgct
    18661 gggcggcctc ttcataggcg cggtttgata aagcctgcgc ttgctcggcc aggtcatctc
    18721 ctttcagatg aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac
    18781 cactgagttg cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg
    18841 cccactcata aatccagttg ccaccacggt agccaatcac agtatcgtat aaatcatcgt
    18901 cggtacgttc agcatcgctc atcacaatac gtgcctggac gtctaggatt tcacgtgggt
    18961 caataccgcg ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt
    19021 taccatcaag agccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg
    19081 tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg
    19141 tcatggatgc agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa
    19201 agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg
    19261 aatacgacgc ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca
    19321 taatcatcaa ccagcggacg accagccggt tttgcaaaga tggtgacaaa gtgcgctttt
    19381 ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg
    19441 atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg
    19501 tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt
    19561 aacgcacccg gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa
    19621 ggcatcagtc ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg
    19681 tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag
    19741 gggaaaatag gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat
    19801 gagtccgcgt ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa
    19861 catatctcgc aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat
    19921 actgttttgc gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc
    19981 acagccgctg tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga
    20041 agagcaactc gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg
    20101 cgatcaggta ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa
    20161 accggtcgtc ttacaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca
    20221 tgacttcacg aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg
    20281 caccacgctg ggtgcagata acggcattgg tatggcgtct gcgctggcgg ttctggctga
    20341 cgaaaacgtg gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat
    20401 ggacggtgcg ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga
    20461 ctccgaagaa gaaggtgaaa tctacatggg ttgtgcgggg ggtatcgact tcacctccaa
    20521 cctgcattta gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa
    20581 aggtctgaaa ggcggtcact ccggcggtga aatccacgtt ggcctgggta atgccaacaa
    20641 actactggtg cgcttcctgg cgggtcatgc ggaagaactg gatctgcgcc ttatcgattt
    20701 caacggcggc acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc
    20761 agctgataaa gtcgacgtcc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa
    20821 cgagctggca gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa
    20881 agctgccctg attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa
    20941 cggtgtgatc cgcaattccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg
    21001 tgtggtgacc atgactgaca ataacgtaga aattcactgc ctgatccgtt cactgatcga
    21061 cagcggtaaa gactacgtgg tgagcatgct ggattcgctg ggtaaactag ctggcgcgaa
    21121 aaccgaagcg aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca
    21181 tctggtacgt gaaacctatc agcgtctgtt caacaaaaca ccgaacatcc agattatcca
    21241 cgcgggcctg gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat
    21301 cgggccaact atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg
    21361 tcattactgg acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga
    21421 tttgctgccg gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat
    21481 aaaagccatc cctgtaaatg tccgtctcgg gttgccacgt tcaatctggt gatggaacat
    21541 tcgccgctgc gatttcagca ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc
    21601 aatcagcaat cgtgccagcc gcttgttagc atgctgacta cgctctgact gaaccttcac
    21661 gctaataccg gatgccaaat gcgtggcgcg taccgccgag tcggttttat tgacatgttg
    21721 accgcccggc cccgacgaac gcaacgtctc ataacggatt gcatccgatt gttcctgctc
    21781 atcagcggta aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata
    21841 cggactcgga caaatccact gaatagtgcc gcaccagctt tcgcttaatg cccatgcgtt
    21901 atcgccatcc agagaaacca gagctgaacg cagcgtgtca gagtagcggc ccgtttctgt
    21961 ttccaacacc gttaccgcga cgtcttgtcg ggcagcttct ttaattagcc tgtccagcgc
    22021 ctttttcact gcgagacaac attcttccgg cccctgcgct gaggagagtt gtagcaagat
    22081 catccttttt tccctccact gtttttgagc gttagcaccg gacgcagccg cgcgacagga
    22141 ataattaaac cggcctgcac cagacattgc accacgcttt cagccgattt ataggcttgt
    22201 ggcgcttctt caaagatgag ttgtttatcg cgacaaatta cccgactgcc aagttcagta
    22261 cgcgagagtt gcgtcgccgt gtatttcgct gccagacgcc ctttacactc ggtgcgcccc
    22321 catttacgcc ctgccccatg cgccagcgag tggagcgttt tttcgttcgc aacaggctgt
    22381 acaatccagg agtaatcacc acgtgaaccg ggaataatca ccagaccgtt gtcatccggc
    22441 gtggcacctt tacgatgcaa ccagccctgt tgatcgccga tttgacacgc gctaacaaag
    22501 ttatgcgcca catccagaac cggacttccc gtggccttaa cttgttgcat tatgcgcaaa
    22561 gcgatcatct ggcgattaat acacgcaaac gccagcgcat cgtcatgttc cgcaatatac
    22621 cgtagcgcgt cgtcactgcc ttgaggcaaa ccatgatgcg agaacgaggc aatatgccgc
    22681 tgtaaaatag actgccccac cccccgcgag ccgctatgaa ccaacagttg cagatgttgt
    22741 gcatccagac ctgccagtgc aaacagttca gcgttgatga tttgatcaac ttgttgcagt
    22801 tcagcgaaat gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca
    22861 aatgctgacg gcaggttttc ctccagccag ctttcttcag caacgtcatc cagatctgat
    22921 aatcgctttt caaacttatc ggcgttgtat ttgcgagtga gaatatctgt ttgccatagc
    22981 gccataccgc agccgatatc attgccgacc agtgccggat aaaaacgacc aacagagaag
    23041 aacgctgcgc caatcggata gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc
    23101 tgcatgttgg gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac
    23161 aggtcatcag atgcgatggt aaataccgca tcggataagg gacgaatata attgcccata
    23221 taccattccg taaatagact cgtcatactt caagttgcat gtgttgcgac ttgaattatt
    23281 gagagtaaca attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
    23341 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
    23401 gcagtttgtg gaataagaaa acgcgcacat actaggcttg cgttattttc attgcaagct
    23461 ggatttaatg ttgctgttta taacgcatat aaaaattaat aaaccactct ccccgacatt
    23521 caacgcgttg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta
    23581 tgcttgcgtc caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgctgg
    23641 caaccccacg gcgggtgtat tcagggtcaa caaataacat atcgatatag tgttcaacgc
    23701 aggtaataaa gccgaccagt tttgcattaa tgactgcaac ccgcacttgt gatttcgcga
    23761 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat
    23821 aatgctgact ggcagtcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat
    23881 cgccaggctg atagtctctt atttgtatgt tattcataat cccagcacca gttgtctttc
    23941 catttgcggg tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg
    24001 gcgttcatcc caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca
    24061 gacgtgctcc tgagtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgggc
    24121 aatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc
    24181 gataatcgct tcacattcag accagtggtg gatatcttcc gccatcgtgc gttccacgcc
    24241 gacggatttt cgcaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc
    24301 ccacaaaatg cggccaaatt tgccaaagcg tttaagcagc atcaccagat cacacttttg
    24361 tacatcaccg caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac
    24421 gccggggatt tttgccagcg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat
    24481 cacaaactga ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg
    24541 tgccacgccc gcagacgccg tcagttgcag ctcgctgaaa atcgtctggc ggatttcctg
    24601 ggcgatgagg gtcgcagaac cgtggcaatg gacgctatcg gtgacgtcga gataagcctc
    24661 atccagtgac aacggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt
    24721 tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt
    24781 gagcgccatc cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc
    24841 ggtgctgatc accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag
    24901 ggcgggattg tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat
    24961 tttacgcatt actcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa
    25021 atgcgtaaag attcagcatt taacgcgttc gctgcgaaag cacctgctgc ttatccagct
    25081 tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat
    25141 cggacgcact tttggtcagc accataatct caatgcgccg gttgcccgcg ctttgcggat
    25201 ttttggaatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg
    25261 gcattccggc ctcttccagc acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc
    25321 agttgttgta gatattgttt ttgtaggcca tcgcatcggt atgcccggta ataataattt
    25381 tgttatcgag cgagtcgaaa actggcgcga gctccaccag cagcctttta aagaacggca
    25441 taatctgcgc gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagcacgc
    25501 gtaatccctg cgggacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa
    25561 tggtgttgat actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagca
    25621 ccgttttctc attgacgtca cgcgcttttt tctccgtctc ttccggcacc gccacggtgg
    25681 caggttttgg cggatgagac gggctgattt tatttaacgg cgacagccca ccaccgttaa
    25741 aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt
    25801 tgacaatcaa ctttattgtc gtcaccaaag gggccgagcg tatttccgag gtttcagccc
    25861 gctttaccct ggatgcgatg cccggtaagc agatggcgat tgacgccgat cttaatgccg
    25921 ggttgatcaa tcaggcgcag gcacaaaccc gacgtaaaga tgttgccagc gaggccgatt
    25981 tctacggcgc gatggacggg gcatcgaaat ttgtgcgcgg ggatgctatc gccgggatga
    26041 tgattctggc gatcaacctg atcggcggcg tctgtatcgg gatcttcaaa tacaacctga
    26101 gcgccgacgc cgccttccag cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc
    26161 aaatcccttc cctgctactc tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca
    26221 acggcgatat cgcccatgac gtgcgtaacc agttgctggc aagcccgtcg gtgctctaca
    26281 ccgctaccgg aattatgttc gtactggcgg tagtgccggg aatgccacat ctgccgtttt
    26341 tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg
    26401 cggaagcgga agagaaaagt ctcgaaacgc tgacccgcac catcaccgaa accagcgagc
    26461 aacaggtcag ttgggaaacc attccgctga tcgagcccat cagcttaagc ctcggctaca
    26521 agctggtggc gctggtggat aaagcgcagg gcaacccgct cacccagcgg attcgcggcg
    26581 tgcggcaggt gatatccgac ggtaacggcg tgctgctgcc ggagatccgt attcgggaaa
    26641 acttccgcct caagcccagc cagtacgcta ttttcatcaa cggcattaag gctgatgaag
    26701 cggatattcc ggcggataaa ctgatggccc taccctccag cgaaacctac ggcgaaattg
    26761 acggcgtgct ggggaacgac ccggcgtacg ggatgccggt gacctggatc cagcccgcgc
    26821 agaaagcgaa ggcgctgaat atggggtatc aggtgatcga cagcgccagc gtgattgcca
    26881 cgcatgtgaa taagattgtg cgtagctata ttcctgattt atttaactat gacgatatta
    26941 cgcagttgca taaccgtttg tcgtcgatgg caccgcgcct ggcggaagat ttaagcgcgg
    27001 cgctgaatta cagccagttg ctgaaagtgt accgcgcgct gctgaccgaa ggcgtttccc
    27061 tgcgcgatat cgtcaccatt gccaccgtgc tggtcgccag tagcgcggtg accaaagatc
    27121 atattctgct ggcagctgat gtgcgcctgg cgctgcggcg cagcattacc catccgttcg
    27181 ttcgcaagca ggagctgacg gtgtataccc tgaataatga gctggaaaat ctgctgacca
    27241 acgtggtgaa tcaggcgcaa cagggcggga aagtgatgct cgacagcgtg ccggtcgatc
    27301 cgaatatgct caaccagttc cagagtacga tgccgcaggt gaaggagcag atgaaagcgg
    27361 cggggaaaga cccggtgctg ctggtgccgc cgcagttgcg ccctttgctg gcacgctatg
    27421 cgaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa tgaagtgccg gatgagctgg
    27481 agttgaagat tatgggagcg ttgagttgat gaggattctt ttccatgctt tgcatgcatc
    27541 tgacaccatt gattgacgtc acattgcaaa catactacga tgacaccata caatgacgtc
    27601 atggattgat agtatgatta aggtcagcgc gctaggctcc cgccatagaa aaacattcga
    27661 cgatgtaatg acggagccgc caaaatctgg aattaaatgg gatgatgttg ttgcgctcat
    27721 taaagcggtg ggtggaacaa taaaaaataa caatgggtcc agaaggaaat ttcagattgg
    27781 aacgaccaaa ttcagtacac atgagccaca tccaaagaat gtgatggata aaggtgcagt
    27841 agcaggtctt aaggagtggt ttgttaactg tgtaggggta gattatgaat aaagcatcga
    27901 acattatcac aattaaaggt caaccagccg cagtgacatt tgaagccgat atcaacgcat
    27961 ttcgcggcaa gttcctcaat gtaaacggtt attgtgactt tgtagccaca agcattgagg
    28021 cattatatcg tgagggcgaa agcgcgctgg atgagtggat ggcggattgt gaagaggacg
    28081 gtattgcccc ttttcgtgaa gaagaagagc aaaaacgcct gacgcttcgt gttcctcatc
    28141 acattgattc gcggctgacc atcgtcgcca gacaacattc catttcaaaa aaccagctca
    28201 ttgttgatgt cttagaacgt gagttttcag cccatatgta aacaagtcgg atgccgcgtg
    28261 gcatccggcc ttttgccagt tcgtcaatat tacccattta caaaatcgtc tcttccgtca
    28321 aaatcctgcg cgcgcccaaa aaatggtcct gccagaaagg ttcggctaac cggcttaccc
    28381 gaatggtttc cccggtgcgc ggcgactcga taaactggcc atcgcccaga tacaccccca
    28441 tatgatcggc aatctcgcgg ctgtggatat ggaaaaacag caaatctccc cggcgcaggt
    28501 cgttattcgc cacaatcgtt gcccgatggt agtgatacat ctcgttggcc gtgcgtggga
    28561 gcttcgcctc aaggatcttg ttgtaggcat agaaaaccaa cccgctacag tcgaagcctt
    28621 gatcgggccg cgtgccgccc catacgtaag gcttgccaag ctgttgctcc agacggtgaa
    28681 tcgctacttc ggtgatgtta tgcagatggt cgctgcttaa gaaatggttg ttttccgcca
    28741 gcgcctgcca gcgtctgtca ctcgccttga gcggcgcagg aaaccattct ggatgctgtt
    28801 ttatctgttc gcggttgtgc tggcgtagcg cccttctgct ttcggcattg ccttccacaa
    28861 tatagctggc ctgttttttc agatgggttt ggtactgttt taataaacgc gcacgatttt
    28921 gcatccgctg gcgggcggcg tagctgaaag tgatatgtga tgtggtggcg ctggcaaatg
    28981 aagaaaacgg caagcaaagc aacagtaatc ccgacaacac aaaagaggga atcgacggca
    29041 aggacatact caataatggc tgatcatgca ttgatgttgc agtgtatccc ggcccgtacc
    29101 tgaacaaact taataaacca ttattataaa attaataaat atatttcagt taattatgta
    29161 aaaacatccg tttattaaat tactcatgga ttgtcgctgt tgctcatttg agctacaatt
    29221 caagctgaat aaatatacag cacaggagat accccaatgg ctgctaacgc atttgttcgc
    29281 gcccgaatcg atgaagatct gaagaatcag gcggcggacg tactggccgg gatggggctg
    29341 accatctctg acctggttcg cataaccctc acaaaggtcg cgcgtgaaaa ggcattgccg
    29401 tttgatttac gcgagcctaa tcaattaacc attcaatcaa tcaaaaacag cgaagctggc
    29461 gttgatgttc ataaggccaa agacgccgat gatttatttg ataaattagg aatttaaata
    29521 tgattcaaag ggatattgaa tactcgggac aattttcaaa ggatgtaaaa cttgcacaaa
    29581 agcgtcataa ggatatgaat aaattgaaat atcttatgac gcttcttatc aataatgctt
    29641 taccgcttcc agctgtttat aaagaccacc cgctgcaagg ttcatggaaa ggttatcgcg
    29701 atgctcatgt cgaaccggac tggatcctga tttacaaact taccgataaa cttttacgat
    29761 ttgagagaac tggaactcac gcggcgctct ttgggtaaag cacgctgccg atgaaatttg
    29821 taaaccctgc gccgacaggc ttgcgtaaaa ctcttttggt gataatgtga gccttccaaa
    29881 tccagactat tccgatttgg gatctatttt gccgtcctgg cgtgtgtaac cgttttatca
    29941 aggaataagc agtatgcgta aaatcgcatt aattcttgcg atgttgttga tcccgtgcgt
    30001 ttcgtttgcc ggtttgctgg gcagcagtag ctcgaccacg ccagtgagca aagagtataa
    30061 gcagcagttg atgggatccc ctgtctacat ccagatcttc aaggaagaac gtacgctcga
    30121 tctctacgtc aaaatgggcg agcaatatca gctgctcgac agttataaaa tctgtaaata
    30181 ttccggcggc ttagggccaa aacagcgtca gggcgatttc aaaagcccgg aagggtttta
    30241 tagcgtccag cgtaatcagt taaaaccaga cagccgttac tacaaagcga ttaatattgg
    30301 tttccccaat gcctatgacc gtgcgcatgg ttatgaaggg aaatacctga tgatccacgg
    30361 cgattgtgtt tccatcggct gctacgcaat gaccaatcag ggtattgatg agatcttcca
    30421 gttcgttact ggtgcgctgg tgtttggtca gccgagcgtg caggtgagta tttacccgtt
    30481 ccgcatgacc gacgccaata tgaagcgcca taaatattcc aactttaagg acttctggga
    30541 gcaactgaag ccgggttacg actactttga gcaaacccgt aagccaccga ccgtttctgt
    30601 ggtcaatggt cgttacgtgg tcagcaagcc gttaagccac gaagttgtgc agccacaact
    30661 ggcatcaaac tacacgctcc ccgaggcaaa ataagcgcca ttcgcctggc ataatctttt
    30721 gccaggtttc attgcccgtc agcggctgtg ttgcaatcac cgtgaccaca tcattcggtg
    30781 tggtctgcga gctgaagtcg atttccacat cctgatccag cagcgttgcc acgccaaacg
    30841 gcgcgcggcg ggtgatccag tgtaaattag tcgagcaata cgccatcaca tagcgcccgt
    30901 cagaaagcag catgttgaaa acgcccttct gccgcagttc atccgccagt gaggcgatat
    30961 atttaaacac tgccgccatg ttgcccggtg tgcgcgggta acgctgcgtt aatttatgca
    31021 ggagccagca gaaggctttt tcgctatcgg tttcaccgac cgggcggaag ttgccggttt
    31081 ccagtgattt gtagcccgtc agttgtccgt tatgggcata agtccagtta cgcccccata
    31141 actcgcgggt aaatgggtga gtattttcca gcgccacctc gccccgatta gcctggcgaa
    31201 tatgagccac caccgaacag gattttatcg ggtagtcctg gacaagtttg gcgatggggg
    31261 aattaaagct gggttgtgga tctttaaatg tgcgacagcc tttaccttcg taaaaggtaa
    31321 tgccccagcc atctttatgt ggcccggttc caccaccacg ctgtacaagt ccggtgaaac
    31381 taaagcagat atcggtaggg acgttggcgc tcatcccgag caattcgcac atgggtaacc
    31441 tccacaacag cgggagcgaa tggcccccgg cgaagattga tttaagaatt ttcaggtcgg
    31501 atgcggcgta aacgaattat tcggcctacg gttcgggtac agatttgtag gcatgataag
    31561 acgcgtcagc atcgcatcag gcatcagcgc acaagtgccg gatgcggcgt aaacgcctta
    31621 tccagcctac gccagactta cttaaccatc tctttttcaa ttaactggat caggatatgg
    31681 atcactttaa tgtgaatctc ctgaatgcgg tcggcataac caaagtgcgg tacgcgaatt
    31741 tcgatatccg ccgtgccagc cattttgccg ccgtctttac cggtcagggt gatcactttc
    31801 atccccttct cacgcgccgc tgcgatcgct ttgatcacgt ttgcagagtt accggaggtg
    31861 gagatcccca gcagtacatc gccttcgcga cctaccgctt caacgtagcg ggagaaaata
    31921 tcattgaaac cgaaatcatt accgacgcag gaaatatgac taacgtcaga aatagcaatc
    31981 gccgggtagc ccggacggtt ttcacggtag cgaccggtca actcttcggc aaagtgcata
    32041 gcgtcgcaat gggaacctcc gttgccgcag gaaagcactt tgccaccggc tttaaagctg
    32101 tctgctaaca ggaccgccgc gcgctgaatg gcgtgaatat tggcgtcatc ttttaaaaag
    32161 ttagccagcg tttccgccgc ttcgttcagt tcgttacgaa taagatcctg gtacatgagg
    32221 atatccttca gcataaatgt aatagacaaa atgcagtgta ccggataccg ccaaaagcga
    32281 gaagtacggg caggtgctat gaccaggact ttttgacctg aagtgcggat aaaaacagca
    32341 acaatgtgag ctttgttgta attatattgt aaacacattg ctaaatgttt ttacatccac
    32401 tacaaccata tcatcacaag tggtcagacc tcctacaagt aaggggcttt tcgttatgat
    32461 gattttgagt attctcgcta cggttgtcct gctcggcgcg ttgttctatc accgcgtgag
    32521 cttatttatc agcagtctga ttttgctcgc ctggacagcc gccctcggcg ttgctggtct
    32581 gtggtcggcg tgggtactgg tgcctctggc cattatcctc gtgccattta actttgcgcc
    32641 tatgcgtaag tcgatgattt ccgcgccggt atttcgcggt ttccgtaagg tgatgccgcc
    32701 gatgtcgcgc actgagaaag aagcgattga tgcgggcacc acctggtggg agggcgactt
    32761 gttccagggc aagccggact ggaaaaagct gcataactat ccgcagccgc gcctgaccgc
    32821 cgaagagcaa gcgtttctcg acggcccggt agaagaagcc tgccggatgg cgaatgattt
    32881 ccagattact catgagctgg cggatctgcc gccggagttg tgggcgtacc ttaaagagca
    32941 tcgcttcttc gcgatgatta tcaaaaaaga gtacggcgga ctggagttct cagcttatgc
    33001 ccagtctcgc gtgctgcaaa aactctccgg cgtgagcggg atcctggcga ttaccgtcgg
    33061 cgtgccgaac tcattaggcc cgggcgagct gctgcaacat tacggcactg acgagcagaa
    33121 agatcactat ctgccgcgtc tggcgcgtgg tcaggagatc ccttgctttg cactgacaag
    33181 cccggaagcg ggttccgatg cgggcgcgat tccggacacc gggattgtct gcatgggcga
    33241 atggcagggc cagcaggtgc tggggatgcg tctgacctgg aacaaacgct atattacgct
    33301 ggcaccgatt gcgactgtgc ttgggctggc gtttaaactc tccgacccgg aaaagctgct
    33361 gggtggcgca gaagatttag gtattacctg tgcgctgatc ccgaccacca cgccgggcgt
    33421 ggaaattggt cgtcgccact tcccgctgaa cgtaccgttc cagaacggac cgacgcgcgg
    33481 taaagatgtc ttcgtaccga tcgattacat catcgggggg ccgaaaatgg ccgggcaagg
    33541 ctggcggatg ctggtggagt gcctctcggt aggccgcggc atcaccctgc cttccaactc
    33601 aaccggcggc gtgaaatcgg tagcgctggc aacgggcgcg tatgctcaca ttcgccgtca
    33661 gttcaaaatc tctatcggta agatggaagg gattgaagag ccgctggcgc gtattgccgg
    33721 taatgcctac gtgatggatg ctgcggcatc gctgattacc tacggcatta tgctcggcga
    33781 aaaacctgcc gtgctgtcgg ctatcgttaa gtatcactgt acccaccgcg ggcagcagtc
    33841 gattattgat gcgatggata tcactggtgg caaaggcatt atgctcgggc agagcaactt
    33901 cctggcgcgt gcttaccagg gcgcaccgat tgccatcacc gttgaagggg ccaacattct
    33961 gacccgcagc atgatgatct tcggacaagg agcgattcgt tgccatccgt acgtgctgga
    34021 agagatggaa gcagcgaaga acaatgacgt caacgcgttc gataaactgt tgttcaaaca
    34081 tatcggtcac gtcggtagca acaaagttcg cagcttctgg ctgggcctga cgcgcggttt
    34141 aaccagcagc acgccaaccg gcgatgccac taaacgctac tatcagcacc tgaaccgcct
    34201 gagcgccaac ctcgccctgc tttctgatgt ctcgatggct gtgctgggcg gcagcctgaa
    34261 acgtcgtgag cgcatctcgg cacgtctggg ggatatttta agccagcttt acctcgcctc
    34321 tgccgttctg aagcgttatg acgacgaagg ccgtaatgaa gccgacctgc cgctggtgca
    34381 ctggggcgta caagatgcgc tgtatcaggc tgaacaggcg atggatgatt tactgcaaaa
    34441 cttcccgaac cgcgtggttg ccgggctgct gaatgtggtg atcttcccga ccggacgtca
    34501 ttatctggca ccttctgaca agctggatca taaagtggcg aagattttac aagtgccgaa
    34561 cgccacccgt tcccgcattg gtcgcggtca gtacctgacg ccgagcgagc ataatccggt
    34621 tggcttgctg gaagaggcgc tggtggatgt gattgccgcc gacccaattc atcagcggat
    34681 ctgtaaagag ctgggtaaaa acctgccgtt tacccgtctg gatgaactgg cgcacaacgc
    34741 gctggcgaag gggctgattg ataaagatga agccgctatt ctggtgaaag ctgaagaaag
    34801 ccgtctgcgc agtattaacg ttgatgactt tgatccggaa gagctggcga cgaagccggt
    34861 aaagttgccg gagaaagtgc ggaaagttga agccgcgtaa ttgaataacg gagccgaaag
    34921 gctccgtttc tttatccgct aattatttaa aattaaagcc atccggatgg ttttccaggc
    34981 tgccggtcaa cgccgcgaac agcaccgttt taccatcaat cgaaagcgca tcgttcacat
    35041 tcagccaggt gagtttctct tgcgacgttt tctcatcaat agtcgagaac agccccgtca
    35101 tctgattaga tttctcggac cacatcacag cgatacgttg cgagccacag tcatgcggtt
    35161 tgcacgcgct catcacctga tacgtctcat ctcccaacgt tacggtttgt gcgggagtat
    35221 aagtaccgcc tttcatcacc caggcaggca gcttatgccc ttgtaccatc tgattaaatg
    35281 cagctttggt ggtttcgccc tttgcaaggc tgctaatggt taaatcatcc tgcgccattg
    35341 cactggtggc gatgaccaga gcggcgactg tcgttattgc cttaaacatc attcctcccg
    35401 agcttatcct gcccatgtta ttaaccttct aaagcctggc gataacacga taatttgcaa
    35461 atcagatatt tccaatatca gcatttgtta tcaatttatt ctgcgctaag gcaactgcct
    35521 gataaggtgt taaagttata aaacccttcg cataaaggag ttgtacaccg tgcctggttt
    35581 gaagattacg cttttgcagc aaccactggt gtggatggat ggccctgcca acctgcgtca
    35641 ttttgatcgt caactggaag gtattaccgg gcgcgatgtg atcgttctac cggagatgtt
    35701 taccagcggc tttgccatgg aagcggcagc ttcgtcgcta gcacaagatg acgtagtgaa
    35761 ctggatgaca gctaaggcgc agcagtgcaa tgcgctgatt gcaggcagtg ttgcattaca
    35821 aacggagtct ggttcggtta accgcttttt gctggttgag ccgggcggca cggtacattt
    35881 ttatgataag cgtcatctgt tccgcatggc agatgagcat ctacattata aagcgggcaa
    35941 tgcgcgagtg attgtggaat ggcgcggctg gcgtattttg ccgctggtgt gctacgactt
    36001 acgttttcct gtgtggtcgc gcaatctcaa cgattatgac ctcgccctgt acgtcgccaa
    36061 ctggcctgct ccgcgctctc tgcactggca ggcattgctg acggcccgcg cgattgagaa
    36121 tcaggcgtat gtggcgggat gcaatcgcgt cggcagcgat ggcaacggct gccattatcg
    36181 cggtgacagc cgggtgatta atccacaagg cgagattatc gctactgccg acgcgcatca
    36241 ggcaacgcgc attgatgccg agctgtcgat ggcggtgtta agggaatatc gcgataagtt
    36301 tccggcatgg cgggatgcgg atgagtttag gttgcggtga gtgggggttg tgtttaaggc
    36361 tgggggagag tcggggtaag actacgaaag cccgctctcc gcaaggactg acgccagata
    36421 gtttctgtcc atagctgcat ttcgcatctt acgtcttaac cctgccttga ataccttatc
    36481 attcgtcaaa atattaatag c
//
LOCUS       DAFAVF010000032        34867 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFAVF010000032 DAFAVF010000000
VERSION     DAFAVF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.032"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1049
                     /locus_tag="LGK42_001558"
     CDS             <1..1049
                     /locus_tag="LGK42_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2657819.1"
                     /translation="FAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIPAGVK
                     TASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLALLAAL
                     FWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIGIARF
                     LADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFHDRGV
                     SLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLGGDLF
                     KKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIALWTLI
                     KVKDEEPQVQLATS"
     gene            complement(1151..1600)
                     /gene="rplI"
                     /locus_tag="LGK42_001559"
     CDS             complement(1151..1600)
                     /gene="rplI"
                     /locus_tag="LGK42_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK2657820.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1642..1869)
                     /gene="rpsR"
                     /locus_tag="LGK42_001560"
     CDS             complement(1642..1869)
                     /gene="rpsR"
                     /locus_tag="LGK42_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK2657821.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1874..2188)
                     /gene="priB"
                     /locus_tag="LGK42_001561"
     CDS             complement(1874..2188)
                     /gene="priB"
                     /locus_tag="LGK42_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBK2657822.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2195..2590)
                     /gene="rpsF"
                     /locus_tag="LGK42_001562"
     CDS             complement(2195..2590)
                     /gene="rpsF"
                     /locus_tag="LGK42_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK2657823.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2918..3193
                     /locus_tag="LGK42_001563"
     CDS             2918..3193
                     /locus_tag="LGK42_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2657824.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3323..4009)
                     /gene="ulaF"
                     /locus_tag="LGK42_001564"
     CDS             complement(3323..4009)
                     /gene="ulaF"
                     /locus_tag="LGK42_001564"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBK2657825.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGAYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4009..4863)
                     /gene="ulaE"
                     /locus_tag="LGK42_001565"
     CDS             complement(4009..4863)
                     /gene="ulaE"
                     /locus_tag="LGK42_001565"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBK2657826.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ARLSRLDWSREQRLVLVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4873..5523)
                     /gene="ulaD"
                     /locus_tag="LGK42_001566"
     CDS             complement(4873..5523)
                     /gene="ulaD"
                     /locus_tag="LGK42_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK2657827.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5537..6001)
                     /gene="ulaC"
                     /locus_tag="LGK42_001567"
     CDS             complement(5537..6001)
                     /gene="ulaC"
                     /locus_tag="LGK42_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBK2657828.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6011..6316)
                     /gene="ulaB"
                     /locus_tag="LGK42_001568"
     CDS             complement(6011..6316)
                     /gene="ulaB"
                     /locus_tag="LGK42_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBK2657829.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6332..7729)
                     /gene="ulaA"
                     /locus_tag="LGK42_001569"
     CDS             complement(6332..7729)
                     /gene="ulaA"
                     /locus_tag="LGK42_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK2657830.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8080..9144
                     /gene="ulaG"
                     /locus_tag="LGK42_001570"
     CDS             8080..9144
                     /gene="ulaG"
                     /locus_tag="LGK42_001570"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK2657831.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9252..10007
                     /gene="ulaR"
                     /locus_tag="LGK42_001571"
     CDS             9252..10007
                     /gene="ulaR"
                     /locus_tag="LGK42_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBK2657832.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10004..10753)
                     /gene="yjfP"
                     /locus_tag="LGK42_001572"
     CDS             complement(10004..10753)
                     /gene="yjfP"
                     /locus_tag="LGK42_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK2657833.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10935..11264
                     /gene="bsmA"
                     /locus_tag="LGK42_001573"
     CDS             10935..11264
                     /gene="bsmA"
                     /locus_tag="LGK42_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBK2657834.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11413..11688
                     /gene="yjfN"
                     /locus_tag="LGK42_001574"
     CDS             11413..11688
                     /gene="yjfN"
                     /locus_tag="LGK42_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBK2657835.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11805..13430)
                     /locus_tag="LGK42_001575"
     CDS             complement(11805..13430)
                     /locus_tag="LGK42_001575"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBK2657836.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13514..14677)
                     /locus_tag="LGK42_001576"
     CDS             complement(13514..14677)
                     /locus_tag="LGK42_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBK2657837.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLM
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14680..15318)
                     /locus_tag="LGK42_001577"
     CDS             complement(14680..15318)
                     /locus_tag="LGK42_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBK2657838.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15328..15726)
                     /locus_tag="LGK42_001578"
     CDS             complement(15328..15726)
                     /locus_tag="LGK42_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBK2657839.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15744..16403)
                     /locus_tag="LGK42_001579"
     CDS             complement(15744..16403)
                     /locus_tag="LGK42_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBK2657840.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16454..17152)
                     /locus_tag="LGK42_001580"
     CDS             complement(16454..17152)
                     /locus_tag="LGK42_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBK2657841.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17171..17572)
                     /locus_tag="LGK42_001581"
     CDS             complement(17171..17572)
                     /locus_tag="LGK42_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBK2657842.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(17699..18430)
                     /gene="rlmB"
                     /locus_tag="LGK42_001582"
     CDS             complement(17699..18430)
                     /gene="rlmB"
                     /locus_tag="LGK42_001582"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK2657843.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18610..21051)
                     /gene="rnr"
                     /locus_tag="LGK42_001583"
     CDS             complement(18610..21051)
                     /gene="rnr"
                     /locus_tag="LGK42_001583"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK2657844.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21090..21515)
                     /gene="nsrR"
                     /locus_tag="LGK42_001584"
     CDS             complement(21090..21515)
                     /gene="nsrR"
                     /locus_tag="LGK42_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBK2657845.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21720..23018)
                     /gene="purA"
                     /locus_tag="LGK42_001585"
     CDS             complement(21720..23018)
                     /gene="purA"
                     /locus_tag="LGK42_001585"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK2657846.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23122..23319)
                     /locus_tag="LGK42_001586"
     CDS             complement(23122..23319)
                     /locus_tag="LGK42_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBK2657847.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23401..24405)
                     /gene="hflC"
                     /locus_tag="LGK42_001587"
     CDS             complement(23401..24405)
                     /gene="hflC"
                     /locus_tag="LGK42_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBK2657848.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24408..25667)
                     /gene="hflK"
                     /locus_tag="LGK42_001588"
     CDS             complement(24408..25667)
                     /gene="hflK"
                     /locus_tag="LGK42_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBK2657849.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(25753..27033)
                     /gene="hflX"
                     /locus_tag="LGK42_001589"
     CDS             complement(25753..27033)
                     /gene="hflX"
                     /locus_tag="LGK42_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK2657850.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27109..27417)
                     /gene="hfq"
                     /locus_tag="LGK42_001590"
     CDS             complement(27109..27417)
                     /gene="hfq"
                     /locus_tag="LGK42_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBK2657851.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27503..28453)
                     /gene="miaA"
                     /locus_tag="LGK42_001591"
     CDS             complement(27503..28453)
                     /gene="miaA"
                     /locus_tag="LGK42_001591"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK2657852.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28446..30293)
                     /gene="mutL"
                     /locus_tag="LGK42_001592"
     CDS             complement(28446..30293)
                     /gene="mutL"
                     /locus_tag="LGK42_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK2657853.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30303..31640)
                     /gene="amiB"
                     /locus_tag="LGK42_001593"
     CDS             complement(30303..31640)
                     /gene="amiB"
                     /locus_tag="LGK42_001593"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBK2657854.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31659..32120)
                     /gene="tsaE"
                     /locus_tag="LGK42_001594"
     CDS             complement(31659..32120)
                     /gene="tsaE"
                     /locus_tag="LGK42_001594"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK2657855.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32092..33639)
                     /gene="nnr"
                     /locus_tag="LGK42_001595"
     CDS             complement(32092..33639)
                     /gene="nnr"
                     /locus_tag="LGK42_001595"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBK2657856.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            33638..34777
                     /gene="queG"
                     /locus_tag="LGK42_001596"
     CDS             33638..34777
                     /gene="queG"
                     /locus_tag="LGK42_001596"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK2657857.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(34760..34813)
                     /locus_tag="LGK42_001597"
     CDS             complement(34760..34813)
                     /locus_tag="LGK42_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657858.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8291 a         9635 c         8592 g         8349 t
ORIGIN      
        1 gttttgcgat atgtgccgca atttggggcc tggcgacaat gggccatgca ctcaccggaa
       61 cgtggtctgg tctggcattt atgcgcgccc tgatgggttt cagcgaagcg tcggcaattc
      121 cggcgggtgt aaaaaccgca tcaacatggt tcccggcaaa agagcgtggc gtggcgacag
      181 gtgttttcaa tatgggcacc tcactcggcg cgatgcttgc tccaccgttg attgcctggt
      241 gcattatgtt tcatagctgg caatttgcgt ttattgtttc aggtagcctt gctttgctcg
      301 cggctttatt ttggttcttt tgttataaag atccgaaaga tgccaaacgc ctttctgatg
      361 aagagcgcca ctatattgaa tcaggacaag aacagcatct taaaacagat aagaaagaaa
      421 aaacgtcaat caagcatatc ctcagccaac gtaatttctg ggggattggc atcgcgcgtt
      481 ttctcgcaga cccggcatgg ggaaccatta acttctgggt gccgattttc ttcgtcgaaa
      541 cgctgcattt tagcctgaaa gaaattgcca tgttcgtctg gctgcctttc ctgctgggcg
      601 atctcggctg tttagccagt ggttttgtcg cgaagttctt ccacgatcgc ggcgtgagtt
      661 taattaactc acgaagaatt accttcacta ttgcagccgt cattatgatg acgattggcc
      721 tggtgagtat tgtcgaaaat ccctacattg ccgtattact gattagtatt ggcgcgttct
      781 cgcatcaatg tctttctact gtagcagcaa ctctgggtgg cgatctgttc aaaaaagacg
      841 aagtagctac cgcagtgggt atggcaggag cctgtgcgtg gagcggtcag ttgattttca
      901 acctgtttat cggggcattc gttcacatta tcggcttcgc gccgttcttt attgccctgg
      961 ctttctttga cattattggc gccattgcgc tgtggacgct tatcaaagtt aaagatgaag
     1021 aaccgcaagt acagttagcg acaagctaat taatagatat cagagcgtta tactaccagt
     1081 tgtaacgctt tcgctataaa gcaaaacgcc gaccaatggt cggcgttttt acgtctcgtt
     1141 gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact tcgctgtgaa
     1201 cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc agacgaactt
     1261 cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg cgagtaccga
     1321 tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca gtttccagtg
     1381 cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt ttagcttcca
     1441 gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga acagctttac
     1501 cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga tcacccaggc
     1561 tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc tctcaaagtc
     1621 gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca gggacaggta
     1681 gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg taccggtgat
     1741 acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag cgatatcttt
     1801 atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac gacggaaata
     1861 acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca tgcaaaacca
     1921 ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg gttatgcgac
     1981 tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca ataacgggca
     2041 tttgacacca tgcctgccgg tgaaaaccgg cttcctcctg cacagaacga tgctcaagca
     2101 cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc ctgcacacgg
     2161 tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc ccagcttcag
     2221 catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg tctttcgctt
     2281 taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg ctgcgaataa
     2341 cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc ggagcttcaa
     2401 cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc agctgacggc
     2461 ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca gcagtgtagc
     2521 gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa acgatttcgt
     2581 aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt cagccgaatc
     2641 ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat agtactgaca
     2701 taccttgcta aactcaaggg cagagcagaa ataaatctcc cctttcgcaa ataaccacta
     2761 agccgctgat ttgaagttca tcacacttca tcatgctgtt tttttgaaca actgcatcag
     2821 aaatggtcgc catcaacttt ttcatccgtg attaaaatta aatcagacca tcaccacatg
     2881 aggagagcac acaaaaacag ccgtcgagga ggtatctatg ttcagccgtg ttttagccct
     2941 tctggctgtg cttttgctaa gtgcaaatac atgggcagcc attgaaatta ataaccacca
     3001 ggccagaaat atggacgacg tacagagctt aggcgtgatt tatatcaacc ataacttcgc
     3061 cactgaaagt gaagctcgcc aggcactgaa tgaagagaca gatgcgcaag gcgcaacgta
     3121 ctaccacgtg attctgatgc gagaaccggg gagtaacggc aatatgcacg ccagcgcgga
     3181 tatttatcgc tagcaccagg tataaccaac gaaacattgc catagtttgc tttgccccct
     3241 tctccggggg catttttttg aaaaacggct gcggaatcag accagttatc tcccgaagaa
     3301 ggaaatttcc gcagcgcgtg ttttacttct gcccgtaata ggcgttggga ccgtgcttac
     3361 gcatgaagtg tttattcatc aggaagctgt cgatgtgatt gagttgtgga ttaatactgc
     3421 gcgcaatcca cgccattttc gccacttctt ccatcactac cgcgttatgc accgcgtcgt
     3481 gggcatcttt cccccaggcg aacggtccgt gctgatacac cacaattccc ggcgtatgca
     3541 gcggctcagc gtcacccagc gtttcgataa tcactttgcc agtgttcagt tcgtactcgc
     3601 cctgcacctc ttcttcgctt aacccgcgcg tacacgggat atcaccaaag aagtagtcag
     3661 cgtgcgtggt gcctaacgcc gggatcgcca gccccgcctg cgcccatgcg gtggcatgag
     3721 tggagtgggt atggacaatg ccgcccaacg acggataacg gcggtagagt tcgagatgcg
     3781 tcgcggtgtc ggaagatggg cgatacgccc cttccaccac cttaccgctc atatcgacca
     3841 ctaccatatc gtctgctttc atggtttcgt aggcgacgcc gctgggcttg atcaccacca
     3901 gcccgcgttc gcggtcaatg gcgctgacgt tgccccaggt aaaggtcacc agcccgtagc
     3961 gcggcagatc catattggct tcaaacacct gctgtttcag cttttgcatt atgccgcctc
     4021 caccatgcct gctttggcca tgcgcgcttt cacccaatcg cgcgctttcg ccacttctgc
     4081 cgccgggtct tccgccgttt cgctccacat ctcaatcagg tacggcccgc aatagccact
     4141 ctgtttgagc gtttcgaagc aacgttcgaa atccactacg ccttcgccaa aaggtacgtt
     4201 tttgaagacg cctggtttgg tgtctttcac atgcaccgcg acgatatgcc cgattccggc
     4261 ctgcaattcc atctgcacat cgttgtccca cgccgacagg ttgccgatat ccgggtagag
     4321 ctggaaccac gggttgttga gatagtgcgc gtagcccagc gccttgctga tggagttcat
     4381 caatgggtaa tccatgatct ccattgccag cgtcacctgc gcgcggcttg ccatctcaac
     4441 gctctctttc agaccgtcac gaaaacgacg acgcgtttcg ttattggctt cctgatagta
     4501 aacgtcatag cccgccagct ggatcacgcg aatgccaacg tcctgggcga actggatagc
     4561 tttgcgcata atctccagcc cctgcgcccg caccgcgtca tcttcgctgc ccagcgggaa
     4621 acgacgatgg gccgaaaggc acatggacgg cacacgcacg ccggtttcaa caatcgcgtt
     4681 gaccagcacc agacgctgct cgcggctcca gtcgaggcgc gacaggcgag cgtccgtttc
     4741 atctaccgac atctcgacaa aatcgaagcc taacgttttt gccagttgca gtcgttccag
     4801 ccagcactcc ccggcgggga gcgctttttc atagatgcca agcgggattt gtttggacaa
     4861 catatccgct ccttagcccc acagttcggc gatggaacgt ttgaactgac gtgcggcttc
     4921 caccggagaa gcggcatcac ggatactgcg acccgcgata aacacatgaa tcggaatacc
     4981 tttgaacagc ggcagatctt ccagcgccaa accaccggtg acggtcactt tgaagcccat
     5041 atcggaaaga cgtttgatcg cggtgatatc tgcttcgccc cacgccacgc ctgcggcctg
     5101 cgcgtcacgg ctgcggtgat aaaccacctg ctgaatacct gcgtcacgcc actgctgcgc
     5161 ctgttcccag gtccagtaac cggtcagttc gatctgcacg tcgccgttaa actcttttgc
     5221 cacgtccagc gcacctttgg cggtgttgat atccgcacag caaattacgg tcacccagtc
     5281 agcattggct tcgaagcaca tacgagaaag gattttgccc gcatcggcaa ttttggcgtc
     5341 tgccagtacg attttgtgcg ggtagagcgc tttcaggtca cgaaccgcac gcacgccttc
     5401 gcccacgcac agaatggtac ccacttcgat aatgtcgact tcttcggcaa tcaggcgagt
     5461 ggtttcgtag gcgctatcca tagtttggtt gtccagcgcg acttgcaaca tcggtaatga
     5521 catgttcaat tccttcttaa gctgccgcgt tggtgcggtc aattaaatcc agtacttcct
     5581 gctcggtacg gcaggcacgt aaacggtcga aattctcttc atcttcaaac aggttgacga
     5641 tctgcatgat acccacttcc tggtgagtgt tggcatcaac cgccgccata gtgatgagga
     5701 tatccaccgg gtcgttatct tcgtggttga actccagcgg ctttttcagc gtcaccagcg
     5761 caaaaccggt tttcttaacg ccctcttccg ggcgtccgtg cggcattgcc agccctggtg
     5821 cgagcacaaa gtacggaccg tgctgttcaa ccgcatccag aatcgcctgg tagtaacgcg
     5881 gctcgacaac atccgccgcc accagcaggt caacgccgat ttttactgcg tcctgccatg
     5941 tctcagcttc agcctgcagg cggatggatt tattctccgc cagcgaatca cgtaatttca
     6001 tggcgcgtcc ttacttcaca tcctgcggga aatgctcttt gatcacttcc agcagtttcg
     6061 ggccaaagtc ggcaggagag agcatgttgc gcacaccaac cacgtatttg ttgccggtca
     6121 cggtgatttc gcccgcaatg tgcgtagaag cgatgatgat atccgcgccg ctcaactcgc
     6181 ttttgtattc gccaaccgcg cagctgttta ccgtgtggtc aatgtttgat tgggttaaaa
     6241 actggtccac tttcatcttc atgatcatgg aactgccttg tccgttgcca cacacagcca
     6301 gaatacgtac ggtcataatc aaaactcctt attaagcaga ctgttctgcc agttgttttt
     6361 ctgcatcttc ttctgcgcgc agtgcgcggc cagcgaagaa catataagcc agtgcaatta
     6421 caatgatgac ggccataaag gcgataccga tggagaagaa gccctgcatc atcggcggtg
     6481 ccagaatcga ccagtccgcc atacccatcc aggcactcat accggtaagt ttcaccgccc
     6541 agacgcaacc aaagatttcg atcatcccca tcaccagaca aatcttcagc gccgcacgcc
     6601 agccgccgaa gtggttagcg aacacgccga tggtggcgtt ggagaagaac atcgggataa
     6661 agccaggaat aatcaggatc gaggaaccgc aggcgaccag gatgccaacc gcaatcagct
     6721 gaccgatagt gccccacata aagccccaga ccacggcgtt cggcgcgaag ctataaatag
     6781 ccgcacagtc aatcgccaga accgcacctg ggatcaggcg ctgggaaatg ccgttaaatg
     6841 cttcagagag ttccgccaca aacatgcgca cgccctgagt gatgatgaag atcgccaccg
     6901 caaaggagaa accagtttgc aggatgtaca ccgtccagtt cactttgcct gccatcgcct
     6961 gcacggtgtc gataccgaag gagagcagaa tggcaccaaa gaagatggtc atgacaatcg
     7021 ccgtggagac gatgttgtcg tggaaaatat tcagccagcc tggcagtttg aggtcttcaa
     7081 cgctttcctc ttttttgccg aggaacggcg caactttata ggcaatccat gatgcaaact
     7141 gctgctggtg accgatggag aaaccgcagc cgtccgtcac ttcctgagtc ggcttgtaca
     7201 tcatgttgga ggtaatgccc cagtagagcg aaaccagaat cgccgtgcag ataatggttg
     7261 tccacatgga gtagccgaaa ataaacaacg taacggcaat cagccccgcc tgctggaaca
     7321 tgatatgacc agtcagcatg atggtacgaa taccggtaat gcgacgcagc agcacgtaac
     7381 agatgttcag cgccagcgct aacaacaccg cgtaacctac ccagctatag gcatcgccca
     7441 tgcggtcgat ggttgccatc atcgacgcgt aagtatcgga aattgcgccg ttaatgccgt
     7501 agacttcgga cattttcgcc accaccggtt tgaaggtgct ggtgaggatg ccggaccctg
     7561 cctgcaacaa catgaaacca attatggttt taatcgtgcc tttaataata acgctgacac
     7621 ttttgcgcag taggatgtag cccagacagg tcacaatacc cagcaacaac ggggcattgg
     7681 tcatgacctg gttaaaaaac acggtaaaga tgttgtagag gatctccata acgatctcct
     7741 gaagaagagg taaccgggta ttccgcacac ccggaatgtt gtgcactcac tctaattttc
     7801 aaaagtaatc acaacaagat tatttatgat taaatgtgac gcgacccgca aataattcca
     7861 cacggataga gtgggattca cacaacgaaa caattattta aattcattta gttaatttaa
     7921 ctacacattc cattaaatct ttccacactc aaaatcaaca acaacttccc tggcaaaaag
     7981 tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag gattaatcaa aaccaatcac
     8041 atattgattc gaattgaact atcatgaagg taaatggcga tgagtaaagt gaaaagtatc
     8101 acccgtgaat cctggatcct gagcactttc ccggagtggg gtagctggtt gaatgaagaa
     8161 attgaacaag aacaggtcgc tcctggcaca tttgcgatgt ggtggcttgg ctgcaccggg
     8221 atctggttaa aatcggaagg tggcaccaac gtttgcgttg atttctggtg cggcactggc
     8281 aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc aaatgcagcg catggctggc
     8341 gtgaaaaaac tgcagccaaa cctgcgcact accccgtttg ttcttgatcc gtttgcgatt
     8401 cgccaggtcg acgcggtact ggcgactcac gatcacaacg atcatatcga cgtcaacgtc
     8461 gctgctgctg tgatgcagaa ctgtgctgat gacgtaccgt tcattggacc gaaaacctgt
     8521 gtggatttgt ggattggctg gggcgtaccg aaagagcgtt gcatcgtggt caaaccgggc
     8581 gatgttgtaa aagtgaaaga cattgaaatt catgcgcttg acgctttcga ccgtactgca
     8641 ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac tgccggacgg catggacgat
     8701 cgcgcggtga actatctgtt caaaacgcct ggcggctccc tgtatcacag cggcgactcc
     8761 cactactcta actattatgc gaagcacggt aacgaacatc agatcgacgt ggctttaggt
     8821 tcatacggcg aaaacccgcg cggtatcacc gacaaaatga ccagcgccga tatgctgcgt
     8881 atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc accacgatat ctggtcaaac
     8941 ttccaggccg atccgcaaga gatccgcgtg ctgtgggaga tgaaaaaaga tcgcctgaag
     9001 tatggcttca agccgtttat ctggcaggtt ggcggcaaat ttacctggcc gttggataaa
     9061 gacaacttcg agtaccacta tccgcgtggt ttcgatgatt gcttcactat tgaaccggat
     9121 ctgccgttca agtcattcct gtaatctgtc gtttataccg gatagcgtgg agtagttcgc
     9181 gctatccggc gtagtatttc ctggctattt caaatatcat ctaaaaaaat caaattttat
     9241 cggaatagct catgactgaa gcacaaagac atcaaatcct cctggaaatg ctcgcacaat
     9301 tgggctttgt gaccgttgag aaagtcgttg agcgtctggg aatttcgcct gccactgcgc
     9361 gacgcgatat caataaactt gacgaaagcg gcaaactgaa aaaagtgcgc aatggcgcag
     9421 aagctattac ccaacagcgc ccgcgctgga cgccgatgaa tctgcatcag gcgcagaatc
     9481 acgatgaaaa agtacgtatc gctaaagcgg cctcgcagct ggttaatccg ggcgaaagcg
     9541 tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg ggaaatgtgt ggcaagccag
     9601 tgcaaatcat cactaattat ctaccgctgg caaattacct gatcgatcaa gaacatgaca
     9661 gcgtgatcat tatgggcgga cagtacaaca aaagtcagtc catcacttta agcccgcagg
     9721 gcagcgaaaa cagtctctat gccgggcact ggatgtttac cagcggcaaa ggactgacag
     9781 cagaagggtt gtataaaacc gatatgctga cggcaatggc agagcagaag atgctgagcg
     9841 tggtagggaa actggtggta ctggttgata gcagtaagat tggcgaacgc gcgggaatgc
     9901 tttttagccg tgccgatcaa attgatatgc tgatcaccgg caaaaatgct aacccggaaa
     9961 tcctgcaaca actggaagcg caaggggtca gcattctgcg tgtttaaaga tgctggcgga
    10021 aaaatgtcac tgcagcatct aacgcctcag gcgtaatgcg gtgacgcacg cctggctgcc
    10081 atgaacaggt tagcagttta tcccgtcccg tctcgcttaa ggcctgctgc aaacgtagtg
    10141 attcgtcggc aggcacaacg tcatcgtcga ggccatgcca cagcagtaga ggtctgtcac
    10201 taagttgttc caggtggttt gtcgcttccc actctgccag cggcgcgaca atgttattga
    10261 attcattctg ctgtgctgcc gtttcaggta tcagcggtgg aaacagtgaa cgggcgagcg
    10321 atgtaaaata gcccgatccc atcatgctgg cggtacattt caccgtgggg tggcgagcgg
    10381 taattcccag tgccgtcatc gcgcccatcg acgcgccacc gactgccaga cggtcatcaa
    10441 gcagccagtt ttcttcggct attgccgcac gtaaagtagt gaattcctgc atactttgta
    10501 gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc gctactaaaa cggctaccgt
    10561 gatcgggcgc atccggcatg atcacccgca aaccagcctg cgccagcgca acggcaaaat
    10621 agctatacac caggctggat gaagtaaagc cgtgataaaa aattacgcac ggtaacgggg
    10681 tatctttttg cccgacagga taagcatgaa gaacgggaat atctgccagc tcgcgtgatt
    10741 ctatttcaat catctgtctc tcctttttgt ctggtatgac atgaagaaat ccttagcaca
    10801 agtttctgct ggcggtatta gttcataatg ttgagatgtg ggttacgctt tcgtcacgtt
    10861 ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca ttcccccaaa gttaaattaa
    10921 taaggcacta cactatggtt agcaggaaac gtaatagcgt tatttaccgg tttgccagtt
    10981 tattattggt gttgatgtta agtgcctgta gcgcactgca aggtacgcca cagccagcac
    11041 cgccagtaac gaaccatccg caagagattc gccgcgacca gacgcaaggg ttacagcgaa
    11101 taggtagcgt aagcacgatg gttcggggtt ctccggatga cgctttagca gaaatcagag
    11161 cgaaagctgt cgctgcaaaa gctgattatt acgttgtcgt catggtggac gaaaccatcg
    11221 tgacaggaca gtggtattca caagccattt tgtaccgtaa ataaatcaga caacttacat
    11281 agtcagcttt acattgcttt gcgtccctat gcgtttacct gtgcacaata aattaccggc
    11341 tggaagcctg ggcgtttatt accgcgatgg aatgccctgc aacatgtggg gaaacgaaaa
    11401 atggagctga cgatgaaaca attacttgcc tcaccctcgc tgcaattagt gacttatcct
    11461 gcgagcgcca cggcacagtc tgcagaattc gccagtgctg actgcgtgac gggcctgaat
    11521 gaaataggtc agatctcagt tagcaatatt tcaggggatc cgcaggacgt ggaacgtatc
    11581 gtggcgttaa aggccgatga acaaggtgcg tcatggtacc gcattattac catgtacgaa
    11641 gaccagcagc ctgataactg gcgcgtacag gcgatcctgt atgcatgagt gtcatctaca
    11701 gcaaatgaac aatgccggat gcggcgcgag cgccttgtcc ggcctacaat tttcacatat
    11761 ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat acgcttacac acacactccc
    11821 cccgtcgccc gcagcaataa atcattctgg atctgctctg acaaccgtac gccgccgcgc
    11881 gtatctaaca tcacctgaca ccacgcctgc gccattggcg gagaggcata tttcaacatt
    11941 tgcgcaccgc agcccagcag gaatagctga tgagtaatct ctcgccccag ttcttcagct
    12001 ggcttacgca gctgctgctg taaacgacga accgcgcgat caaaatagcg atcctgccct
    12061 ttcacttcca caaatgcttc cgacaataag tcgtatacgc ccgcttgttt attgagaacg
    12121 cgcagcacat ccaggcacat aatattgccg gaaccttccc aaatactgtt taccggcatc
    12181 tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac caatgccgcc cagcacctcc
    12241 atcgcttcgg ccacaaacgg catacctctt ttgcagatca caaattttgc cgcaggtgta
    12301 aataaacgcg cccacagagc ttctttggca tcggcacgcc ggtcccacgc tcgcgcaaga
    12361 cgaaacagca acgccgtttg cccttcaagc tgaagtgcca tgcgacttaa gacatgacgc
    12421 ataaggggct gttggatcaa tggattacca aaaacatggc gttgatgtgc atgataaatc
    12481 gccagcgaga acgcacggcg catcatggca tggctaccca gggcgcaatc aaaacgcgtc
    12541 atcccaccca ttttcaggat cagacgaatt ccttcccctt cctgccccaa caaccagcca
    12601 atggcatcct gaaactccac ttcgcagctg gcgttagagc gattacccag cttatctttc
    12661 agccgctcga ggcgaatcgc gttgcgttgc ccgtcaggca aaaagcgcgg cacaaaaaag
    12721 caggacaaac cgcccgtagt ctgcgccagc accagatgcg catcgctttg cggcaccgag
    12781 aaaaaccatt tatgccccac cagccgataa gagccatctt ccaggcgctc tgcgcgggtg
    12841 gtgttgctca tgacatcgga accgccctgc ttttccgtca ttcccatgcc aatcaacaaa
    12901 ccgcgttttt gcccacctgg caataagtga gaatcgtagc gatcgctcag cagcggcgtg
    12961 gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca atggcgtggc ggcaaaggtc
    13021 atggttatcg gacataacga ccccgcctca acctgcgcat gtaacataaa acgcgccgcg
    13081 cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg caagattgtg cacccgattg
    13141 gtacatagcg cctgcatcag caggtgccag gcggggtgaa aacgcacatc gtccagacgg
    13201 cgtccttgcg catcgtagcg caataattca ggcggattca cattcgccag ccgccccagt
    13261 tcaagggatt cagccgttcc taactgctga ccaatactgg ctagaaaatc gctatcccag
    13321 ccagcacctt cacgcgttac cgcttcgcag agcgcgccat cagacaggta taagttgctg
    13381 ttatttaatg gtataggttg attaaaaacg gtgtgagttt gccagtgcac tgtgtctccc
    13441 tccatcaatg gcagtcacct ttagtatggt cactgccatg gattcctgac agaaagagat
    13501 tgctaaaaca ttcttagcca gcaatgtaat gcggaacgaa acgagaggtg tctttggtga
    13561 tcagcgtgtt atcttcacgg atccccattc cgcacgcttc atcatcgaca atccagctac
    13621 cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg aaacgcctgg tagatcatcg
    13681 gttcatcggc gtaatcacca tcagcgtggt caacgacgtt attctgaccg tcgaaaatgg
    13741 tgacgttgcc gccttcgcgc gagtagatcg gtttacgcac atagctttcg ccagcggcaa
    13801 tctgcggttt ttcaccttca aaccaggatg caagaaggtt aggatgacca gggaagaagc
    13861 gccaaagcag cggcattagc cctttattac tcaagatact tttccataac ggctccaccc
    13921 attgctcgcg acgcttgcgc agcagcggac cgttatcgtc acgcatcatc cactccagcg
    13981 gatacagctt aaatgcacgc tggatgacat tatcatcaag atcggtcagt acgccgccga
    14041 cgcccaaacc gagatcttca atgtagataa accgcgactc ctgccctgcc tgctgggcgc
    14101 agtcctgcaa gtacagcacg gtagtcctgt cttcatcggt gtcctgacag cagcaaaaat
    14161 aaaacggttc ccgactgtaa agctcactaa agcgcgacat caggcgttcc tgaatggcat
    14221 tgtactgatc ggcatcacgc ggaataatgc cgctgcgccg ggcatcctcc agccacaacc
    14281 actggaaata agccgactca tacaacgagg tcggcgtatc ggcgttgtac tccagcagct
    14341 tcaccggcgc attgccacac caggcaaaat ccatccggcc atacagcgaa ggatcgcgtg
    14401 cgcgccagct ttcggcgatc acatcccagt acaacggcgg gatcgccagt tgcgtcagga
    14461 tctcttcgtc tttaaccgcg cgatccacca cctcaaggca catctgatgc agttccgcag
    14521 tcggtttttc gatctgctct tcaatctggc gcagagtaaa acggtaagcc cgactctcat
    14581 cccaatagat ttcattgtcg atgatatgaa agtcgaaacc gttgttagcg gcgatctggt
    14641 ccagatcccg tcgcacagga acgttgtgtc tcagcatgat tagcctcccc aatgcccacg
    14701 ggcgctggaa gaacgaccat agccgccgcg cgaaacggtt gacgcttttt tggtggtaaa
    14761 gccgcctgaa gagtaagact cttttttgcc agaaccggag cgccaggagt aatcaccgga
    14821 agtgttgcgc cagacggggc gggaagcaaa tgaggatccg ccgctgttat aaacaaactg
    14881 ctcatcgcga tctttacgaa taacccggct taacaaaaat ccagatatta ccgggatcca
    14941 actctgttca acgttgtcgt agtaacaatt ttcatactta gactgacagt tctgctgagt
    15001 catattcttc ggaacatcgg cataaaacgc cgctttggcg ttattccagc cacgcgcgca
    15061 aatgtcggca ttattaccgt catcaataca atcctgcact gtcgagtaaa acgttccgtc
    15121 gccgtcgtta tcgacatcgc tactttcact ccatcctttg actattaaaa aaacggcacc
    15181 gcccattagc accaacgtta agtatttttg tgcgtagcga ttgcggcgcg gctcaaaagg
    15241 attatgcggt cttccaatgc gactgatcgc tccgtgacca attttactgt tatttcttga
    15301 tttgcgtttt ctggccatga tcgtccatta ccatgtcata caagctgcgt taaaaatacc
    15361 gccagccagc gcggcggttc ccataaacat tcctgctgcg gtattgtgat taataatttt
    15421 ttcgcttaat gcgggcatat aaagtctgac accagcaaaa acgagtaact gaatcaccag
    15481 tgcaatcccg ccccaggcga aatagtctgg aatactcacc gcattgattg ccgcactgga
    15541 taatgggata acgtaaccta acaatgtacc actgaacgcc agtgaagccg cggtattatt
    15601 gtttttgatt aactgccatt cgttgtgcgg tgtaatttta gaatagataa aaagaaaaat
    15661 aatcaccata gccacgccaa taaaaaaata ggcgctaaag gcaagaagtg aatccagtat
    15721 atgcattgta aacgtccttg taattaacca ataatatgaa gtgatgtcag cgggatatcg
    15781 acacccaacg cgcgcgaaaa taaccactct ggctcgccga gatcgttaaa agattcctca
    15841 ccatttaaca gcaaatattc gtaagtatct tcggttactt ggcgttggta gcccatggta
    15901 aaattatgga cctcccattt ggcatggttt tgattttcta ctttttccag catgtagacg
    15961 ggttcgatat ttcccggttc ttcgctgtta aaaaatcgct gccagcgttt ttcctgccag
    16021 tttaagatca ttgcccccat cactttggcg ttgatcgcct cgcgccagtg actctcttta
    16081 ctgataccgt agctctcttc atagacaaag agcttgatat catcaatgtc atcaatatct
    16141 tcgccgccgg tggtattgat ttgcagaaat tcatcgcctg aagtgtagta gcggaaaatc
    16201 tgactaccgc cgcccagatc gatgtggctg acagcggcta ccgtaaattc ctcacccggc
    16261 agcgcaatca gcaattcatc ttccagcaaa cgaaacgcta acgtgtcgag cgtaaagcca
    16321 ctattgagat gaagtcccag cggaccgcga gcgatagcag gcttattatc cttgccaaac
    16381 agacgctgga aaaaaccaga catattttat ccttaaaata gttgccagcc ttttagcgag
    16441 gctggcaaaa aaattactcg ccctgttggc gttgcagtct tgctaataca tcctgggcgc
    16501 tacttttatt gctaccgcca attccggctt ccgccagctt ttcatcaaga tcgcgaccgt
    16561 ctgcgacttt ttccaactgt gcagcagcat ccaggcgagc ctgacgttcg gcctgacgcg
    16621 tttgcaggcg ttttaaggat tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag
    16681 aggttgttac agcctgttgt gcacgctgca tggcttcagt ggctttaacg acttccattt
    16741 gctgctcaaa ctgagcgata cgctgcgctg tcgctgtaac tgctttttcc acgccatcac
    16801 gggagacttc cagattcgac aacacttgct cttcagcggt aatgagattc tcaaggcgcg
    16861 cgatttcttc agcaacttcg ttaatcaacg acggattaac gttcttgctt aacgcttcca
    16921 gcgcacgggc ttccagactg gctttgcgct cacgtaaatc tttcagctta tcgtgactta
    16981 atttcacccg cgccagcaga tcaacgcgag attttccggc tttatcgagt tcagctttag
    17041 cgtcacgaat atgctgttcc agcatgcgca cgccctgggt ttcttcaatg gattcttccg
    17101 cctgggagat aaacgatttc cccagcgtaa ataaactttt taaaattccc ataaataccc
    17161 gtccttgtaa ttagtgtgaa tattcttctg taatttcagc cagatccaat gcgttatcta
    17221 ccagcgaggt tatctcaagc aggatatctt caagagaaga tttaagcgat aacgccccga
    17281 aaacaatgta atactcttcc tgttgcacgc tggagattcc taccgatgac aacggcatca
    17341 tcttctgatt tcttaataag aaggtattaa attcatctgg attgctgata ctacttaccg
    17401 gacaaataaa ggtttcgata atcatttggc gggaagtaaa aagaatattg atttgcaaat
    17461 cgccataatc attcatttta ataattaatg cgctctcgct atttgtcaca tcaatatttt
    17521 gttcaggtac agtttgcagc gccgtcgcca gcgccaacgg attccatgtc atatattatt
    17581 ccatatagat ttagtttaaa tattaataaa atgaatattt gcagtacgta attatcttac
    17641 cagctataga caaaaaaaaa ccatccaaat ctggatggct tttcataatt cagacgaact
    17701 agctgcgctg acgaaccgct tcaaataagc aaattccggt tgcaaccgaa acgttcaggg
    17761 aagaaacact tcctgccatt gggatgctga tcaactcatc gcaatgttca cgggtcaggc
    17821 gacgcatacc ttcaccttcc gcgcccatca ccagcgccag gcgtccggtc attttgctct
    17881 gatagagtgt atgatccgcc tcgcctgccg taccgacgat ccagatattc tcttcctgca
    17941 acatacgcat ggtgcgcgca aggttagtca cccgaatcag tggaacgctt tctgccgcgc
    18001 cgcaggctac ttttttcgcc gtagcgttga gctgtgcgga gcgatctttc ggcacaatca
    18061 ccgcgtgaac gccagcagcg tccgcgctac gcaggcacgc gccgaggttg tgcggatcgg
    18121 ttacaccgtc gaggatcagc aggaacggtt gatcgagcga agcgatcaga tccggcagat
    18181 cgttttcctg atactgacgt cctggcttca cgcgggcgat aatgccctga tgcacggcac
    18241 cgtcgctttt ctcgtcgaga tattggcggt ttgccaactg gataaccacg ccctgagact
    18301 caagggcgtg aatcagcggt aacagacgtt tatcttcacg gccttttaaa ataaagactt
    18361 cctgaaaacg ttcaggggcg cgctccagca gggcctgcac tgcgtggatg ccgtaaatca
    18421 tttcgctcat taatgtactc gttgtttacg ttttgcctga tgcactacgt ttatccggcc
    18481 tacatgatct ctgcaatata ttgaatttgc gtgattatgt aggccggata aggcgttcac
    18541 gccgcatccg gcatgaacaa agcgcatttt gtcagcaatc taaccctctt cttttaaaga
    18601 gggtattgat cactctgcca cttttttctt cgccgcacgc ttcgctttgg tcgctgcggc
    18661 tattttctgc gttttcgccg atggcttttt cgcttttctc gcgtctttct tcgccgcttt
    18721 cggcttcgtt tttttctcac cgcggaaggc gctgtctggc tcaaagttta cctttttacc
    18781 gacctgacga cgcttgccgc cttttttgcc tgcatcgcct tttttcgctt tctcgcgcgc
    18841 cgttttaccg acgttgcgcg gtgcgcgttc actggagatc agactaaagt cgattttgcg
    18901 ctcgtccata ttaaccgctt cgacgcgaac ttccacgcga tcgcccaggc gataagtctg
    18961 gccgctggat tcccccatca ggcgttgccc tacctggtca aagcgatagt agtcattgtc
    19021 cagcgaagag acatggacca gaccatcaat gaacaagtcg tccagacgga cgaagaagcc
    19081 aaagccagtg acgctggaaa ttacgccttt aaagacgtta cctacctggt cgagcatgaa
    19141 gtcacacttc agccagtcag cgacatcgcg cgttgcttcg tcggcacgac gttccgccat
    19201 cgaacagtgc tgacccagtt gcaacatctc ttccatcgaa taatggtagc cgccggtttc
    19261 agtggtgttg ccctgatgcc cctgctcttt cgccagcaga tatttaatgg cgcggtgcag
    19321 cgtcaggtca ggataacgac gaatcggcga agtaaagtgc gcataggact gcaatgccag
    19381 accgaagtga ccacggtttt ctggatcgta aatcgcctgt ttcatcgagc gtagcagcat
    19441 ggtttgcagc atttctgcat caggacggtc ggcaaccgac tccagcagtt ccgcgtagtc
    19501 acgcggttcc ggcttgttac cacccggcag ctccagcccc agctccgcca gcactgaacg
    19561 gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata cggaacagtg ccggttcttt
    19621 cgctttctca acgaaacgcg ccgccgagat attcgccaga atcatgcact cttcaattaa
    19681 tttgtgcgcg tcgttacgct gggtctgttc gatacgttca atacggcgtt cagcgttgaa
    19741 aatgaacttc gcttcttcgc tctcaaatga gatcccaccg cgttcttcac gggctttatc
    19801 cagcacttta tagaggttat gcaactcttc gagatgctta accagcgggg cgtactgctc
    19861 gcgcagatcc tgatcgccct gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg
    19921 agagctcatc accgcttcgt agaatttgta gcccgtcagg cggcctttcg acgaaaccgt
    19981 catctcgcac accatacaca ggcggtctac ctgcgggttg agcgagcaca ggccgttaga
    20041 gagcacttcc ggcagcatcg ggataacctg cgaagggaag tacaccgacg taccacgatt
    20101 acgcgcttct ctgtccagcg gcgttggcgg acgcacatag tagctgacgt cggcaatcgc
    20161 gacccataaa cgccagccgc cgccgcgttt tttctcgcag taaactgcat cgtcaaagtc
    20221 acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg cgcaaatcga cacggcccgc
    20281 ttttgcttct tccggcactt cttctttcag cccggcaacc tgttgctcaa cagcctgcgg
    20341 ccagatgtac ggaatttcat gggtacgcag agcgatatca accgccatgc cggtgcccat
    20401 attatcgcca aggacttcga cgattttacc caccgctttg gtacggcgag tcgggcgttg
    20461 ggtcagttca acaaccacca caaagcccat ccgcgcgccc atgatctgat cgggcgggat
    20521 taagatatcg aagctcagac ggctgtcgtc aggaaccaca aagccgacgc ccgcttcggt
    20581 aaagtagcga ccaacaatct ggctggtttt tggcaccagt acacggacaa tacgcgcttc
    20641 acgacgacct ttacggtcag cgcccagcgg ctgcgccagc acctgatcgc catgaatgca
    20701 ggttttcatc tgctcgctgg agagatacaa atcatcttta cgcccttcaa cccgcagaaa
    20761 gccgtagcca tcacggtggc caataacggt acctttcacc aggtcgaggc gttccggcag
    20821 cgcatagcac tgacgacgag tgaagaccag ttgaccatcg cgctccatcg cgcgcaggcg
    20881 gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc
    20941 acggctggcc ggtttttcac gtttggttaa atgttcgagg ataaattccc gactagggat
    21001 gggattcgcg tatttttcag cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc
    21061 tccgttgtca tctctgatga agattttcgt cactccacca gcaataattt ataaagcggt
    21121 tgattctctt caaccaaatc ggcaagcgtg tagttatcca gttccgtaag aaaactttgc
    21181 acggccttag aaagcgcctg tttcaaccga caggcaggtg taatgtggca aaactcactg
    21241 ctgcaattca ccagcgataa gggctccagc tcgcgcacca catcaccaat acgtatcgca
    21301 ctcgccggtt tacccaggcg aatgccgcca ttttttccac gaacagcagt cacgtagccg
    21361 gcacgactaa gttgattgat tattttgacc atatgattac gggagacgcc gtagacgtca
    21421 gtcacttcag aaatactggt catccgccct tctggcaatg acgccatgta gatcagcgca
    21481 cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg gaaatcggga
    21541 aaaaatacat ttatattgat gataaaccag ccacaagctg tgtcgctaat ttatttcagt
    21601 tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga
    21661 cacagcaatg tatcacactg ttagcccggc aggcaaaata tctgccaggc gtaccagaat
    21721 tacgcgtcga acggatcgcg cagaatcatg gtttcagtac gatccggacc ggtagagatg
    21781 atatcgatcg gcacaccagt cagctcttca atacgcttga tgtagttcag cgccgcctgc
    21841 ggcagaccgc tacgatcttt cacgccgaag gtggattcag accagcccgg catggtttcg
    21901 taaatcggct ctacaccttt ccagtcgtca gctgccagcg gagtggtagt cacttcgcga
    21961 ccatccggca tacggtaagc cacgcagagt ttaacctctt tcaggccatc cagaacgtcc
    22021 agtttagtca ggcagaagcc agacagggag ttcagctgta ccgcacgacg aacggcaacg
    22081 gtgtccagcc agccggtacg acgacgacgc cccgtagttg cgccgaattc gttaccctgc
    22141 ttgcagagga actcgccagt ttcatcaaac agttcggtcg ggaacggacc tgcacctaca
    22201 cgagtggagt aagctttgag gatacccaga acgtaatcaa cataacgcgg gcccaggccg
    22261 gaaccggtcg ccacgccacc agcagtggtg ttggaagaag ttacgtacgg ataagtaccg
    22321 tggtcgatat ccagcagcgt accctgcgca ccttcaaaca tgacgaaatc gccacgctga
    22381 cgcgcctggt cgagcaggtc agaaacgtca accaccatag aagtcaggat gtcggcaaca
    22441 gccatcgtat catccagaac tttctggtaa tcaaccgctt cagctttgta gtagttaacc
    22501 aactggaagt tgtgatattc catcacttct ttcagttttt cagcgaaggt ttctttgtcg
    22561 aaaaggtcgc caacacgcag accgcgacgt gctactttat cttcataagc aggcccgata
    22621 ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc
    22681 gcaacgtgat aatcaaggat cagcggacat gcttcagaca gcagcagacg ctcacgaacg
    22741 gggatgccac ggtcttccag ttctttcatc tctttcatca gcgcggccgg agacagcaca
    22801 acaccgttac cgatgatgct ggttacattc tcgcggagaa tacctgatgg aataagatgg
    22861 agaacggttt tttcaccgtt gattacgaga gtatggcctg cgttgtgacc gccctggtag
    22921 cgtacaacat atttagcccg ttcagtcaga agatcgacga tcttaccttt accttcgtca
    22981 ccccattggg tgcccagtac gacgacgttg ttacccattt ttcaaaatca ccgtttgctt
    23041 aaaaatggat tctaccatcg ctttttcaga gttacagcac tttttgcact caaaaatgac
    23101 ctcgatcagt ctgttttttg ttcagccaat cgttttcctc aacatgtagt agaccacaac
    23161 gcccgcaacc acaagtccac cgccaaaacg acgtaaaata ttatcgggca aattggtcat
    23221 cgcagagatc atcttcttcc atgccttcgg gtaaagcatc ggccctaaac cttccagtac
    23281 caaaaccagg gcaagcgcca gccagattgt cgaattcatt cattatcctt atagaaaaag
    23341 aaaaccaccg acatccctga ggatgcggtg gctttattga cctgtaccgc agtcgttata
    23401 ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag aaatcgctat ccgggctcat
    23461 gaccatcacg tcctgattgc cagagaagct gttctcataa gcacgcaggc tacggatgaa
    23521 tgcgtagaag tccggatctt tactgaatgc atcagcaaac agtttggctg cttcggcatc
    23581 gccttcacca cgcatgatgc ggccctgacg ctcagcttct gccagcgttc tggtcacttc
    23641 atagtcggca gtcgcgcgca gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg
    23701 cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg
    23761 caggttgatc tgcttgatac gcacatcgac aacttcaata cccagcgccg ccatactgtt
    23821 cgggttgatg accggaactt tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc
    23881 aatggcgtta tccgctgccg gggtagtaac ttcatcttct gtacccgcag aaccggagtt
    23941 cagcgcgtca cgtacttcga gggtcagacg accacgggaa tcggtgacga tgtctttcac
    24001 gtccaggcga ccaatttcag aacgcagacg gtcagagaac ttacgtttca acagcacttc
    24061 cgcttgcgaa atgtcgccac cacccgttgc caggtagtaa cggctgaaat cgctgatgcg
    24121 ccatttgatg taagagtcga cgatcaggtc tttcttctct ttggtcacaa agcggtcggc
    24181 ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc accgtttcaa tgaacggtat
    24241 cttgaaatgc agacccggct cataaaccag aggtttgttg tcatcgtcac gcagtacctt
    24301 accaaaacgc agcgtaatac cgcgctcacc ttctttgacg acaaagacag acatgtaaag
    24361 cactaccagc acgatgatga taatcgcgat aactgactta cgcatcgtta ttccccctga
    24421 cgctggtagt cgttacgctg cgcgttggcg cggcgttggt ccataatatc gccctgactg
    24481 gtggacgacg tgttgcttgc tccactggtt gtggaggaag aggctggcgg caaacgcagc
    24541 agattgctgg caccgttatc gctcttcgcc gcaggggcgt taccaccttt cagcatctgg
    24601 tctaacggca gaaccatcag gttgccacct ttatcgttaa ccagcacttt gcgggtgtta
    24661 cccaacactt tttccatcgt ctcgatatac agacgctcgc gagtaatttc cggcgcggct
    24721 ttatattccg gcagaagttt agcaaagcgc gccacttcac cctgagcttc caggatggtc
    24781 tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc
    24841 tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg
    24901 gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg gacgagcagc ctggaagttg
    24961 acgtccagca gcgtgatacc catgtcatac ggacggatag tctcttccag ttcgcgctga
    25021 gtatcgctac gaatcacggt acgaccttcc gtcagaatgc ggtccatggt gtatttaccg
    25081 ataaccccgc gcagggcgct gtcggtagcc tgacgcagac tgtcatccgg gctggtcacg
    25141 ctatacagat atttttccgg attggtgacg cggtactgca cgttcatctc aacgcgcact
    25201 acgttctcgt ccgacgtcaa catcacaccg gaagcggcca gttcacgcac ggcttccacg
    25261 tttaccggtt tgacttcgtc gataaacgtc ggtttccagt tcagacccgg ctcaaccaga
    25321 tggctgaatt taccaaagcg tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa
    25381 ccactggccg cccagataat gacaatcgct gccgctgcga tggtaacgac acgaccgcca
    25441 agctgcgggc gcgggccttg cgatgaactg ccaccgccag atccggtgcc tttaccgccg
    25501 cccagaccac cgagcttttt gctcagtttg cggaagatat catctaaatc aggtggccct
    25561 tgatcgcgac cgcctttgtt tccatttccc tcagagttgc cgccaggttt gctgcttccc
    25621 cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc acgccatgtt tgtgctccat
    25681 atttgttatg cggtgatccc tgttgtcttt cgacgccaga ggatatgact ccacgcttca
    25741 gacgctacgc cgttagatca ggtaatcgat caacgccggt tcttgtttac agaggcgacg
    25801 ccagtcaacg atcggcatac gaacttgcag acttacgctg ccgtcctcct ccatccactc
    25861 tttttctatt gcctgaagct gataaaaacg acttctcaga cgcccttcct gcggtggcag
    25921 acgcaatgta tgctgcgcca cctcgccgga aagccgctcc gtcaaagcct gaaaaagctg
    25981 tggtatcccc gctccggtct gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc
    26041 ccgatcaata cgcggttcga aatcttccag catatcgatc ttgttcatca ccagcagggt
    26101 tgggatctcg tgagcgtcga tctcttcaag aaccgtattc accgcttcga tgttttcttg
    26161 tacacgcaca tccgccgcat caatgacgtg cagcagtaat gtggcttgcc gcgtctcttg
    26221 taacgtggct ttaaatgctg caaccagatc gtgcggcagg tggcgaataa accctacggt
    26281 atctgccaac acggtttcac cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag
    26341 ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt
    26401 agatttaccg gcgttggtat atcccaccag cgaaacagta ggaacgtcgg ctttgatacg
    26461 cgattgccgc ccctgctcac gctgcttttc aactctttcc aggcgcgact gtatctgcac
    26521 gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa
    26581 acctatcccg cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc gcgtagccag
    26641 atggcgcagc tgcgccagct caacctgcaa cttaccctca tgggtacgcg cacgttgggc
    26701 gaaaatatct aaaataaggc cggtgcggtc gataacacga cactcgcaca aacgctccag
    26761 gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc
    26821 tttgacagct tccgcaattt caactgcttt accttcacct acaaaatact ttgggtgcgg
    26881 cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag agaccagaga
    26941 ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta aaatagatgt gtaccagtac
    27001 cgcctgctca ccagcatcat aacggtcaaa caagcgtata accctctaaa tagatcagcg
    27061 gggaacgcag gatcgctggc tccccgtgta aaaaaacagc ccgaaacctt attcggtttc
    27121 ttcgctgtcc tgttgcgcgg aagtattctg cgcgctgcta ccatgatggt agttactgct
    27181 ggtaccgcca ccggcgttgt tactgtgatg agaaaccgga cgagacggga caacagtaga
    27241 aatcgcgtgc ttgtaaacca tctggctgac cgtgtttttc aacaggatca cgaactgatc
    27301 aaaagactcg atttgccctt gcagcttaat accattcacc aaataaatag aaactggaac
    27361 acgttcccga cgcagtgcgt tcaggaacgg atcttgtaaa gattgcccct tagccattct
    27421 ctcttttcct tatatgctta tttgtacttt gaacctttcg attctgaaaa aattgcgcac
    27481 gatacgtctc aattgtacac attcagcctg cgatagcacc aacaacctgt aatacttcgt
    27541 cacgcgcctg ttctggtttt tcactgtcaa gccagtgaac cccttcccaa ccacgcagcc
    27601 aggttatctg ccgcttcgcc aactgtctcg tggcgcaaac acctcgataa accatttcat
    27661 cgtatgagat ttcgccttca aggtaagacc acatctggcg ataacccacg caacgaatgg
    27721 aaggcaagtc cgtatgcaaa tctcctcggg caaaaagcgc ccggacttct gcttcaaaac
    27781 ctgaagccaa catctgatga aaacgctgct caatgcgttg atggagcagt tcacggctcg
    27841 ccggggcgat ggcgaactga tgcacctgat acggtagagc gtctcctgac gtttgcgtca
    27901 gttccgttaa agttttaccc gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg
    27961 gatcatttgg atgaatcctt gctgccgcaa ccggatctac ctcctgaagt tgacgatgca
    28021 atgactccca accttgctct gccgcctgtt gctcaattct ggcccgcact tccgggtctg
    28081 ccgacggtag cggcgacaac ccttccagca atgccttgaa atacaacatc gtaccgccca
    28141 ctaacagtgg gatccgcccc gccgcggtga tatcggccat ttccgccagc gcatcgcggc
    28201 gaaaatcagc agccgagtaa gcctgcgacg gatcgcgaat atccagcaat cggtgcggcg
    28261 cggcgagtaa ctcttcagcg ttcggcttcg ccgtcccgat atccatccct ttgtaaataa
    28321 gggcagaatc aacgcttatc aactctactg gtaaaatttt acgcagctca atggctaacg
    28381 ccgttttacc ggaggccgtc ggccccatca aaaaaatcgc cttaggcagg ctcgccttac
    28441 tgatatcact catctttcag ggcttttatc gccggatgta aatcaacaga ttgtaacaga
    28501 ccacccggcg gcgttttcac aagttgcgga cataaccgtt ccacgtccgc cagcagggtt
    28561 atggcctgtg ccattgacca ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc
    28621 gcaatattgc caggttcgaa tacggactgc ttcgccaggt agcctatcag ttcaggaatc
    28681 aagatttgta aattttgttg gcgtaagggt aaaggcacgg ccctgatggt cacatgctgt
    28741 gcatctgact ggaaatcaat acccaattcc gccagggcag actgcgcttt ttctaatgcc
    28801 gatttttctt cgccagaaac ttttagccgc aacggaatca gcaacggctg ggcgcaaact
    28861 ggcgcttcac ccggcgtcag ttgcgcctga cgcagccaac gttctgccac tggcaaggct
    28921 aaaagtgaaa tgttgccgtc gcgctccagc aacgcacagt cggaatggac gatagtcagt
    28981 acccgaccaa aactctgact gttcgccgca agtgcaggtt cctgcggttc cggcgctttt
    29041 ggtttttgca tcggcgcggg cgtttgcaaa agctggcgat acacttcacc ttgctgtttc
    29101 tggtagcctg gctgcgcatt cggccagggg gcagccggac gactgcctga tgctggcgca
    29161 ggagtgtaac gcggagctac cggctcccga gctgccggtt ctgcaaagtg attgcgcccc
    29221 gccgccacgc ggttttccgg gatggcacgc ggtgcaggtt ggggttcatc gtccagcggt
    29281 agcggcgttt ccagttgctg ctgtaacacg ctcagcacgc cttgatagat aaagtcatgc
    29341 accagacgcg actgatgaaa acgcacttcg tgtttggcgg ggtgcacgtt gacgtccacc
    29401 tgatgtgggt cgatctccag atacagcaca aatgccggtt gctgatcggc ccccagtttg
    29461 tcttcgcagg cctggcggat cgcgtgattg atcaggcgat cgcgcatcat gcgaccgttc
    29521 acgtagcaat actgaatttc tgccagtgcg ggcgtggtgt gatttggatc ggccacccag
    29581 ccgcgtagcg tgagatcgcc gtgttgccat tcaatcgcca gcgcttgttc aagaaaagcg
    29641 gtgccgcaaa tcgcgcctaa gcgccgttct ttttgcccgc cttccggcac tgcgcggtac
    29701 tgacgcacaa ttttaccgtt atgcgacagg ttgatcgtga cgtcgaaacg cgccagcgca
    29761 atgcggcgga tgatctcatc aatgtggtta aattcggttt tctcggtgcg caggaatttg
    29821 cgccgcgccg gggtgttgta gaacagatcc agcacctcca gcgtcgtccc cacaggatgc
    29881 gccgccggtt ttaccgtcac gttcatatcg cgcccttcgg cataggcctg ccaggcttcc
    29941 tgctgttctg cggtgcgtga agtgagcgtc aggcgggaaa ccgaactgat actcgccagc
    30001 gcctcaccgc gaaagcccag gctgataatg gcttcgagat cgtccagaga ggcgatttta
    30061 ctggtggcat gacgcgccag cgccagcgcc agctcatctt ttttgatacc gcagccgtta
    30121 tcacgaatgc ggataagttt cgccccaccg cgttcgatat caatatcgat acgcgtcgcg
    30181 cctgcatcga ggctgttttc caccagttct ttgactaccg acgcaggtcg ctcgaccacc
    30241 tcacctgcgg caatctggtt cgccagttgt ggcggtaaga cctgaattgg catcaatcgt
    30301 ccttagtttg gcagcgtgcg atctggcgtc gtcaccgtac tggcagtttg tgccgttgcc
    30361 ccctgcggcg cagattgcat cggatgcgca aggaaataat tgcgcaggcc tttgtaaatg
    30421 gcttctgcca gctgttgttg gtaatcgtcg ctcgccagca aacgttcttc gctgttgttg
    30481 ctgataaaac cggtttcgac cagtactgac gggatatccg gcgaacgcag aacgccgaga
    30541 ctcgcgtgtt ctggtcggcg tttatgtatt tcgccaatgc gttgcaactg actgatcata
    30601 ctggtcgcta catcataccc tacccgttgg gaatgaccga actgtaaatc cagcaccgcc
    30661 tggcttaaat aggggtcaga ctgactgttc gccagcacat cacccgcccc acccagcagc
    30721 tccgactgtt tctcgtgctg ctccagccag ctggccattt cgctgttggc gcgacggtta
    30781 gagagcaccc atacggaagc gccagtcgca ctgcggttcg gtgcggcatc agcgtgaatc
    30841 gacacgagga aattggcgtt ttgcttacgt gccacatcac tgcgccccat caccgagata
    30901 aagtaatccc cgtcacgggt taaaacgccc ttaaacatcg gatcgtcatt gagcaaagta
    30961 cgcaatttac gcgcgatggc gatggtgaca tttttctccc gcgtaccacc ggggccgata
    31021 gcgccagggt cctgaccgcc gtgtccggca tcaatagcga tgataatttt atcgccagtg
    31081 ttagccgtcg cgcgcgctgc cggacgcgtt accgtattac tgctgataac acccgtagtg
    31141 cggttacttt ccgttttaaa cggattgcgc gccggttcgc tgacgcgcgg tgcgacaacc
    31201 gcaggcgttt caacgcgttt cgcaaccacg ggcggcggag gaggcggtgg cggcacatcg
    31261 gcgttaatcg taaagacgac agtgtaattg ctgccattct gccgcttcac cgcttcggtt
    31321 ttaccgtttt cggtaagatc gaccaccagc cgtagcgttt gtgcatcttt aggcgttcca
    31381 gagcgaatcg ccttcaccag attattgccg ctgaacaaca acggcagtcc ctgaatcacg
    31441 cccgtttgtt tgatatcgag cgccacggtg cgtttgcttt gatggctaaa cgcataatca
    31501 ggatcgccaa taaaactcaa cgttatccgc gcctgttgat taccgttaga aacctgaata
    31561 tcagagagcg tcgcggcacc caccggcgtg cacagcagca gcagcgtcgc taccaaccaa
    31621 tttctgatgc gatacatcat cccgccacct ttcaaaggtt aaccggctaa acgcgccagc
    31681 aacaattcac ccgcagagga aaccgcactc acgcgcgcct cacggccttg tgcctgataa
    31741 tcaatgtgta tttcgacatc cgggtcagga agaacacctg taccttgttg tggccactcc
    31801 accaggcaga tggcatcgtt ggcaaaataa tcgcggatcc ccataaactc cagctcctcg
    31861 ggatcggcaa ggcggtacaa atcaaagtga tagaccatta agttgtcgag cgtatagggt
    31921 tcgaccagcg tataagtggg gcttttgaca ttaccctgat gacccagagc ctgtaaaaag
    31981 ccccggctaa aggtggtttt acctgcgcct aaatcgccat acagatagat tacggttgcg
    32041 ccatcgcagg ctttcgctac ccgctcgccc aggtctaatg ttgcctgctc atcagggagc
    32101 ggaattactc gattcatcat ggtttttatc agtcacttcc gggttaacaa tacgctgtag
    32161 cgtggaaaag agatcggttg ccagcatccc gcgcgttcca aaacgcgccg ccagtacgtc
    32221 agctgccgca ccgtgcgcga cacagcctgc acaggctgca tcatacggcg acagtttttg
    32281 cccaagcaat gcgccaataa taccagagag cacatcgccc atgccgccgc tcgccatgcc
    32341 tgcatttccg gcatcaataa tgcctaaagc gtcaggatgg gcggcgacca cggttccggc
    32401 acctttcagc accgctacgc cgccataacg ttgtaccaga cgtttggcgc aatgtaagcg
    32461 gtcactttca atttcagcga cggaacagcc taacaaccgt gcggcctcgc caggatgcgg
    32521 cgtgatcacg cgattgtgac gcttatcggg attgattgcc agcaggttca atgcatcggc
    32581 atcccacaac atcggtttgc gaaaattctc aactttttgc agtgcttttt tcccccactc
    32641 ttgctggccc agaccgggac caatcaccac cacatcggcc cattccaggc tttcggtaag
    32701 agagtccatc gtcagttcat gcaccatcaa ttccggtcgt gcagtcagca gcggcgcaat
    32761 gttttcactg cgggtcagta ctcggactaa accagcacca gcacgcagcg ccgcttcccc
    32821 cgtcatacga atagcccccg ccgtgccgtg atcgccaccg ataattacca gccgcccgtg
    32881 atcgccttta tgcgaagtcg ggcgacgcgg ttttagccag tgagaaagtt gttctgctga
    32941 aaaccgctga attttcgtct cctgacctgc cagccaacta tccagcccca gtgagtcaaa
    33001 atgcagttgt ccggtaacat cccgcgcttt tccagtgagc aagcctggtt tcagcgcaat
    33061 aaaagtgatg gtgtgatcgg cgttgatcac cgcgcctggc gtagcgccag tttcagccag
    33121 caggccggaa gggatatcaa ccgccacaat cggcgcagga tgggaattag cgtggtcgat
    33181 taactggcta atggattcgc ggggcgcttg ccgcaaaccg gtaccgagca gcgcatcaac
    33241 aatcagatct accgattcgg gccagacaat attcgaagca tggatctcgc cacccgcgtt
    33301 taaccatgct tcgcgtgcca gcgcggcctc ttccggcaac ggtttgtcgc tctcctgggc
    33361 caacaacgtg acctcaatgc cgaccgcttt ggccagtcgc gcgaccacgt agccatcgcc
    33421 gccgttatta ccatgaccgc acagcaccag ccagtggcgg gcgtcaggat acgccgaacg
    33481 acacacctgg aatgcggcct cgccagcgcg aagcatcagc tcatagagtg tgagccccag
    33541 cacatctgcc gcctcgcgtt ctccgcggcg gatatcgtcg gcgtaccaga cggtgtgtgg
    33601 tatacttacg gggtttttct tcattgtatg gtccgtcatg tcagagcccc tcgatctcaa
    33661 tcagttagcg caaaaaatta aacagtgggg gctggaactg ggctttcagc aggtaggtat
    33721 taccgatacc gatctcagcg agtccgagcc caaactgcaa gcatggctgg acaaacaata
    33781 ccacggcgaa atggactgga tggcacgtca cggtatgctg cgcgctcgcc ctcatgagtt
    33841 attgcccggt acgctgcgcg tgatcagcgt gcggatgaat taccttcctg ctaacgccgc
    33901 atttgccagc acgctgaaaa accccaaact cggctatgtt agccgttatg cgctgggccg
    33961 tgactatcac aaacttctgc gcaaccgact caaaaagctg ggcgagatga ttcagcaaca
    34021 ttgtgtttcg ctgaatttta gaccgtttgt cgattctgcg cctattctcg agcgcccgtt
    34081 agctgaaaaa gctgggctcg gctggacagg taagcactca cttatcctca atcgcgaggc
    34141 cggttcgttc ttctttttag gcgaattgct ggtcgatatt ccgctgcccg tggatcaacc
    34201 agtcgaggaa ggatgcggca aatgcgtggc ctgtatgacg atttgcccga ccggtgccat
    34261 cgtcgagcca tataccgtcg atgctcgccg ctgtatctct tatctcacca tcgaacttga
    34321 aggggcgatc ccggaagagt tgcgaccgtt aatgggaaac cgtatttacg gttgcgatga
    34381 ctgccagctt atctgcccgt ggaatcgcta ttcacaactc accacagaag aggatttcag
    34441 cccgcgtaag ccgctacacg caccggaact cattgagtta ttcgcctgga gcgaagagaa
    34501 gtttttaaaa gtcacggaag gatcggcgat tcgtcgtatt ggtcacctgc gttggctgcg
    34561 taatatcgcc gtagcattag gcaatgcccc ttgggatgaa acgattttga cagcgctgga
    34621 aagtcgtaaa ggtgagcacc cacttctcga tgagcacata gcgtgggcga ttgcgcagca
    34681 aatagagaga cgaaatgcgt gcatagtcga agtgcaattg ccgaaaaaac agcgtctggt
    34741 tcgggtgatt gaaaaagggt taccgcgtga cgcctgattc atccacagct tgtgaataaa
    34801 aataaaaacg cattgcaatt caagacgaat aaaatcgtca agtgatcgac tcgacaaaac
    34861 ggaatct
//
LOCUS       DAFAVF010000033        34569 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFAVF010000033 DAFAVF010000000
VERSION     DAFAVF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.033"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..2130
                     /locus_tag="LGK42_001598"
     CDS             <1..2130
                     /locus_tag="LGK42_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBK2657859.1"
                     /translation="HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYV
                     LTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMIL
                     GSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVA
                     LAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHN
                     WLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTD
                     ILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQ
                     SCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGL
                     SIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEY
                     AFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTT
                     DELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFA
                     RIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRY
                     RSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITL
                     YMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2272..3555)
                     /locus_tag="LGK42_001599"
     CDS             complement(2272..3555)
                     /locus_tag="LGK42_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBK2657860.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3707..4183)
                     /locus_tag="LGK42_001600"
     CDS             complement(3707..4183)
                     /locus_tag="LGK42_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBK2657861.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(4242..5531)
                     /gene="bioA"
                     /locus_tag="LGK42_001601"
     CDS             complement(4242..5531)
                     /gene="bioA"
                     /locus_tag="LGK42_001601"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBK2657862.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARHRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            5618..6658
                     /gene="bioB"
                     /locus_tag="LGK42_001602"
     CDS             5618..6658
                     /gene="bioB"
                     /locus_tag="LGK42_001602"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBK2657863.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            6655..7809
                     /gene="bioF"
                     /locus_tag="LGK42_001603"
     CDS             6655..7809
                     /gene="bioF"
                     /locus_tag="LGK42_001603"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBK2657864.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            7796..8551
                     /gene="bioC"
                     /locus_tag="LGK42_001604"
     CDS             7796..8551
                     /gene="bioC"
                     /locus_tag="LGK42_001604"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBK2657865.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            8544..9221
                     /gene="bioD"
                     /locus_tag="LGK42_001605"
     CDS             8544..9221
                     /gene="bioD"
                     /locus_tag="LGK42_001605"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBK2657866.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            9800..11821
                     /gene="uvrB"
                     /locus_tag="LGK42_001606"
     CDS             9800..11821
                     /gene="uvrB"
                     /locus_tag="LGK42_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBK2657867.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(12013..12921)
                     /gene="yvcK"
                     /locus_tag="LGK42_001607"
     CDS             complement(12013..12921)
                     /gene="yvcK"
                     /locus_tag="LGK42_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBK2657868.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      13186..13330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            13318..14307
                     /gene="moaA"
                     /locus_tag="LGK42_001608"
     CDS             13318..14307
                     /gene="moaA"
                     /locus_tag="LGK42_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBK2657869.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            14329..14841
                     /gene="moaB"
                     /locus_tag="LGK42_001609"
     CDS             14329..14841
                     /gene="moaB"
                     /locus_tag="LGK42_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBK2657870.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            14844..15329
                     /gene="moaC"
                     /locus_tag="LGK42_001610"
     CDS             14844..15329
                     /gene="moaC"
                     /locus_tag="LGK42_001610"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBK2657871.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            15322..15567
                     /gene="moaD"
                     /locus_tag="LGK42_001611"
     CDS             15322..15567
                     /gene="moaD"
                     /locus_tag="LGK42_001611"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBK2657872.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            15569..16021
                     /gene="moaE"
                     /locus_tag="LGK42_001612"
     CDS             15569..16021
                     /gene="moaE"
                     /locus_tag="LGK42_001612"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBK2657873.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            16158..16862
                     /locus_tag="LGK42_001613"
     CDS             16158..16862
                     /locus_tag="LGK42_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2657874.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            17064..17711
                     /locus_tag="LGK42_001614"
     CDS             17064..17711
                     /locus_tag="LGK42_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2657875.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            17781..18494
                     /locus_tag="LGK42_001615"
     CDS             17781..18494
                     /locus_tag="LGK42_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK2657876.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(18530..19486)
                     /locus_tag="LGK42_001616"
     CDS             complement(18530..19486)
                     /locus_tag="LGK42_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBK2657877.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(19486..20727)
                     /gene="clsB"
                     /locus_tag="LGK42_001617"
     CDS             complement(19486..20727)
                     /gene="clsB"
                     /locus_tag="LGK42_001617"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBK2657878.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(20724..21485)
                     /locus_tag="LGK42_001618"
     CDS             complement(20724..21485)
                     /locus_tag="LGK42_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK2657879.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            21618..22028
                     /locus_tag="LGK42_001619"
     CDS             21618..22028
                     /locus_tag="LGK42_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBK2657880.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(21990..23096)
                     /locus_tag="LGK42_001620"
     CDS             complement(21990..23096)
                     /locus_tag="LGK42_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2657881.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23107..24240)
                     /locus_tag="LGK42_001621"
     CDS             complement(23107..24240)
                     /locus_tag="LGK42_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2657882.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(24233..25969)
                     /locus_tag="LGK42_001622"
     CDS             complement(24233..25969)
                     /locus_tag="LGK42_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2657883.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(25962..26957)
                     /gene="hlyD"
                     /locus_tag="LGK42_001623"
     CDS             complement(25962..26957)
                     /gene="hlyD"
                     /locus_tag="LGK42_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBK2657884.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(26960..27631)
                     /gene="cecR"
                     /locus_tag="LGK42_001624"
     CDS             complement(26960..27631)
                     /gene="cecR"
                     /locus_tag="LGK42_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBK2657885.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAECLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            27860..29224
                     /gene="rhlE"
                     /locus_tag="LGK42_001625"
     CDS             27860..29224
                     /gene="rhlE"
                     /locus_tag="LGK42_001625"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBK2657886.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(29456..29938)
                     /gene="ybiA"
                     /locus_tag="LGK42_001626"
     CDS             complement(29456..29938)
                     /gene="ybiA"
                     /locus_tag="LGK42_001626"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBK2657887.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            30058..32208
                     /gene="dinG"
                     /locus_tag="LGK42_001627"
     CDS             30058..32208
                     /gene="dinG"
                     /locus_tag="LGK42_001627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBK2657888.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            32236..33198
                     /gene="ybiB"
                     /locus_tag="LGK42_001628"
     CDS             32236..33198
                     /gene="ybiB"
                     /locus_tag="LGK42_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBK2657889.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            33339..34424
                     /gene="hcxB"
                     /locus_tag="LGK42_001629"
     CDS             33339..34424
                     /gene="hcxB"
                     /locus_tag="LGK42_001629"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBK2657890.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         8180 a         8965 c         9367 g         8057 t
ORIGIN      
        1 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
       61 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggtatgga acgtttcccg
      121 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      181 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      241 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggcaggcgg cggcaaaatg
      301 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      361 ggcggtggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccggga
      421 gttgttgcgg tgcatctgac cggaaaacca gcgccgtatg tggggccgca ggatgtggcg
      481 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
      541 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
      601 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
      661 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
      721 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
      781 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
      841 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
      901 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
      961 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1021 ttctcgctgg cagtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt
     1081 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcgttctg cggcccatgc
     1141 tttggcgcgg gcgatacgcc aatcaacaac ggattaagta ttcgccacac cacgcgtaac
     1201 ttcccgaacc gcgaaggctc taagccagct aatgggcaga tgtcagcggt ggcgttgatg
     1261 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1321 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1381 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1441 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1501 atcctcgacg aagtgaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     1561 cgttcaaatc cgattggtct ggcggagttt accctgtcac gccgcgatcc cggttatgtt
     1621 ggcagaagta aagcgactgc tgagctggaa aatcagcgtc tggcggggaa tgtcagcgag
     1681 ctgacagagg tgtttgcgcg cattaagcag attgctggtc aggagcatat tgatccgctg
     1741 caaactgaaa ttggcagcat ggtatatgcg gtgaaaccag gcgatggttc tgcgcgtgaa
     1801 caggcggcga gctgccagcg tgtgattggc ggtctggcga atattgccga agagtacgcg
     1861 actaaacgct accgttctaa cgtcatcaac tgggggatgt taccgctgca gatggcggaa
     1921 gtgccaacct ttgaagtggg ggattacatt tacatccctg gcattaaagc ggcgctggat
     1981 aatccgggta cgacgtttaa aggttatgtg atccatgaag atgcgccggt aacggaaatt
     2041 acgctctata tggaaagtct gactgctgaa gagcgcgaga ttatcaaggc gggtagtttg
     2101 attaacttca ataaaaaccg tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg
     2161 gcacttattg tcggatgcga tgcttgcgca tcttatccga cctacgaatc gcatcgaatc
     2221 tgtaggccgg ataaggcgtt tacgccgcat ccggcaaata gttaatagct cttacttctt
     2281 cgcctctgca accactttac tacccacgcc gcggttattg tattcccaca tgcggttgta
     2341 gttagtgtca ttcagattgc gctgtatttc gtcgttatca tctacgctgc cggtattacc
     2401 cgcaaacgga cgattagaga tcaccgcatc ggcccacggt ttagccgtgt taaaaccttc
     2461 gttgatggcg ctatcacgga tcaccacctg accgttggta ttggcatcaa catccagcga
     2521 gcggcccagt tgcgccacac catcaccgaa agcattgaaa cggctgttta cggcgaggaa
     2581 accgtagtaa atgttggaca gcgtagccgg tgcaaacaca tacgcttctt gctgagtacg
     2641 tgagttcacc acgcggaatt cggtgttatc gaacaccact gcgccgcgac cagaaacgat
     2701 atccacatcc ccttcaatgt agctgttggt caccagcgta cgcggctgac ggttggtttc
     2761 cagacggttc tgcacaccgc tgttggtgac aaagaaggtg ttctgacgac cgagaatgtt
     2821 aacgttgtta atctgtacct ggtcaccatc agtacgcagt gccaccgccg gatggttacc
     2881 tgcatctacg ctatcgccca gcgtgttttc gatggtcaga ttttgcagtt gcaggccatt
     2941 gttttgtgac cagaagaccg cagagcagag aacaccgata ctgtcgctgc gtttgctctg
     3001 gcagctatcg tacatatacc acgctggttt acctggcata tatttgccgc gcgggttgac
     3061 gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg gaaagcccaa tcttcacatc
     3121 aatcggtttt tcacctgtac cgtacagagt aattccaccc ggagcagcag ggacatacac
     3181 cgttccctga tactcaccag gcatcacggc aatatactgg cgcttgttag tgcgcttgat
     3241 aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt acaccttgag tacccgccgg
     3301 gccgacaaca aagtcaggtt gcgcaggcag ggtaatcggg gaaggattcc acgctgcagc
     3361 acctggtgtc agggatgcaa aatagtgttg agcatcgaaa ttctgcgctt cttttgccga
     3421 cagaatcggg cgagaagagg taccaggcgc ggtttgatca gaaggacgtt gatcgggtgg
     3481 tgttgagcta caggcggtca gcgtcacgcc aaaagccaat gccagcgcca gacgggaaac
     3541 tgaaaatgtg ttcacaggtt gctccgggct atgaaataga aaaatgaatc cgttgaagcc
     3601 tgctttttta tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa
     3661 tacctttaag tgataccaga tggcattgcg ccatctggca gagtgattaa ctaaacatcg
     3721 cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc atcgcgccgc
     3781 tggcaccttc atcgacatca atacgttcta tatccagcgc gtgaacggta aaaatgtagc
     3841 gatgagtttc gcctttcggc ggtgctgcgc catcgtaccc ggttttacca aagtcggtac
     3901 gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata
     3961 cgcgggtatc agcgggtaag ttaacaacta cccagtgcca ccagccggag ccggttggcg
     4021 catccgggtc gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca tcatcccacg
     4081 ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgcg
     4141 gcaatttatc gccatcgcgc agatcgttac tgatgagttt catgaaccct cctttcttgt
     4201 ttgcagaaag tgtagccaga aaccctcacg cggacttctc gttattggca aaaaaatgtt
     4261 tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg cgggagaata
     4321 atatagggcg gcatcaggta aatcagtttg ccaaaaggcc ggatccagac accctgttcg
     4381 acaaagaatt tttgcagcgc cgccatattc accggacgag tggtttcgac tacgccgatt
     4441 gcccccagca cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc aagttgctcg
     4501 cgcagctgta cttcaatagc cgccacctgt tgctgccagt cgccagattc gagaatcgcc
     4561 aggctggcgt ttgctgccgc gcaggccagc ggattgccca taaaagttgg cccatgcata
     4621 aagcaaccgg cttcgccgtt actgatggtt tccgcaacct cgcgcgtggt gagcgtggcg
     4681 gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgatt
     4741 tctgcatgtt cacaggcaaa cagtttgccg gtacgaccaa atccggtggc gatctcgtcg
     4801 gcaatcagca agataccttc gcgatcgcac attttgcgga ttcgttttaa ccattccgga
     4861 tggtacatgc gcatcccgcc tgcgccctgg acaatcggtt caatgatcac cgccgcgatt
     4921 tcatgacgat gcgccgccat caggcgggca aagccaacca tatcacgttc atcccattca
     4981 ccatccatgc ggctttgcgg ggcgggagca aacaagtttt ctggcagata gcctttccac
     5041 aggctgtgca ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga
     5101 taaccattgc ggaaggtcag aaaacggtgg cgcgcttcgc ctttggcctg ccagtactgc
     5161 aacgccattt tcatcgccac ttccaccgct acggagccgg agtccgcgag aaaaacgcat
     5221 tccagcggtt gcggcgtcat tgccaccagt ttgcggcata gctcgatggc gggcgcatgg
     5281 gtgataccgc caaacatcac atgcgacata gcatcaattt gcgacttcat cgccgcatta
     5341 agctgcgggt gattatagcc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg
     5401 cgtctgccgt cagacaaaat tagctcgcaa ccttcggcgc tcgcgaccgg ataaaccggc
     5461 agaggggagg tcatggatgt gtatgggtgc cagatatggc gttggtcaaa ggcaagatcg
     5521 tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac aagtctacac
     5581 cgaattaaca acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac
     5641 attgtcgcaa gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca
     5701 gcaggtgcat cgtcagcatt tcgatccacg tcaggtgcag gtcagcacgt tgctgtcgat
     5761 taagaccgga gcttgtccgg aagattgcaa atactgcccg caaagctcgc gctacaaaac
     5821 cgggctggaa gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgcaaagc
     5881 gaaagcggca ggatcgacgc gcttctgtat gggcgcggcg tggaagaatc cccacgaacg
     5941 cgatatgccg tacctggaac aaatggtgca gggggtaaaa gcgatggggc tggaggcgtg
     6001 tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc ctcgcgaacg ccgggctgga
     6061 ttactacaac cacaacctcg acacctcgcc ggagttttac ggcaatatca tcaccacccg
     6121 cacttatcag gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg
     6181 ttctggcggc attgtgggct taggcgaaac ggtaaaagat cgcgccggat tattgctgca
     6241 actggcaaac ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg tgaaggtgaa
     6301 aggcacgccg ctggcggata acgatgatgt cgatgccttt gattttattc gcaccattgc
     6361 ggtcgcgcgg atcatgatgc cgacctctta cgtgcgcctt tctgccggac gcgagcagat
     6421 gaacgaacag actcaggcga tgtgctttat ggcaggcgca aactcgattt tctacggttg
     6481 caaactgctg accacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact
     6541 ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcaacgtct
     6601 tgaacaggcg ctgatgaccc cggacaccga cgaatattac aacgcggcag cattatgagc
     6661 tggcaggaga aaatcaacgc ggcgctcgat gcgcggcgtg ctgccgatgc cctgcgtcgc
     6721 cgttatccag tggcgcaagg agccggacgc tggctggtgg cggatgatcg ccagtatctg
     6781 aacttttcca gtaacgatta tttaggttta agccatcatc cgcaaattat ccgtgcctgg
     6841 cagcaggggg cggagcaatt tggcatcggt agcggcggtt ccggtcacgt cagcggttat
     6901 agcgttgcgc atcaggcgct ggaagaagaa ctggccgagt ggctgggcta ttcgcgggca
     6961 ctgctattta tctctggttt tgccgctaat caggcggtca ttgccgcgat gatggcaaaa
     7021 gaggaccgca ttgtcgctga ccggcttagc catgcctcat tgctggaggc tgccagttta
     7081 agcccgtcgc agcttcgccg ttttgttcat aacgatgtca ctcatctggc gcgactgctt
     7141 gcttccccct gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc
     7201 gatattgcgc cactggcgga aatccagcag gtaacgcaac agcacaatgg ctggttgatg
     7261 gtcgatgatg cccacggcac gggcgttatc ggggagcagg ggcgcggcag ctgctggctg
     7321 caaaaggtaa aaccagaatt gctggtggtg acttttggca aaggatttgg cgtcagtggg
     7381 gctgcggtgc tttgctccag tacggtggcg gattatctgc tgcaattcgc ccgccacctt
     7441 atctacagca ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc actggcggtc
     7501 attcgccgtg atgaggggga tgcacggcgc gaaaaactgg tgtcactcat tgcgcgtttt
     7561 cgtgccggag tacaggattt gccgtttacg ctggctgatt catgcagcgc catccagcca
     7621 ttgatcgtcg gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc
     7681 tgctgggtca cggcgattcg cccgccaacc gtacccgctg gtactgcgcg actgcgctta
     7741 acgctaaccg cagcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc
     7801 aacggttaat aaacaagcca ttgcagcggc atttggtcgg gcagccgcac tctatgagca
     7861 acacgcagat ctacagcgcc agagtgctga cgtcttactg gcaatgctcc cacagcgtaa
     7921 atacacccac gtactggacg cgggttgtgg acctggctgg atgagccgcc actggcggga
     7981 acgtcacgcg caggtgacgg ccttagatct ctcgccgcca atgcttgttc aggcacgcca
     8041 gaaggatgcc gcagaccatt atctggcggg agatatcgaa tccctgccgt tagcgactgc
     8101 gacgttcgat cttgcatgga gcaatctcgc agtgcagtgg tgcggtaatt tatccacggc
     8161 actccgcgag ctgtatcggg tggtgcgccc cggaggcgtg gtggcgttta ccacgctggt
     8221 gcagggatcg ttacccgaac tgcatcaggc gtggcaggcg gtggacgagc gtccgcatgc
     8281 taatcgcttt ttaccgccag atgaaatcga acagtcactg aacggcgtgc attatcaaca
     8341 tcatattcag cccatcacgc tgtggtttga tgatgcgctc agtgccatgc gttcgctgaa
     8401 aggcattggt gccacgcatc ttcatgaagg gcgcgacccg cgaatattaa cgcgttcgca
     8461 gttgcagcga ttgcaactgg cttggccgca acaacagggg cgatatcctc tgacgtatca
     8521 tctttttttg ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg
     8581 aagtggggaa aaccgtcgcc agttgtgcac ttttacaagc cgcaaaggca gtaggctacc
     8641 ggacggcagg ttataaaccg gtcgcctctg gcagtgaaaa gaccccggaa ggcttacgta
     8701 atagcgacgc gctggcgtta cagcgcaaca gcagcctgca gctggattac gcaacggtaa
     8761 atccttacac cttcgcagaa cccacctcgc cgcacatcat cagcgcgcaa gagggcaggc
     8821 cgatagaatc attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg
     8881 tgttagtgga aggcgctggc ggctggttta cgccgctttc tgacactttc acttttgcag
     8941 attgggtaac acaggaacaa ctgccggtaa tactggtagt tggtgtgaaa cttggctgta
     9001 ttaatcacgc gatgttaact gcacaggcaa tacaacacgc cggactgact ctggcgggtt
     9061 gggtggcgaa tgatgtcacg cctcctggaa aacgtcacgc tgaatatatg accacgctca
     9121 cccgcatgat tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa
     9181 atgcggcaac tggaaagtac ataaaccttg ccttgttgta gccatcctgt atttggttaa
     9241 attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tctaaaataa
     9301 tcacgaaaaa aattttactt ccgcatcatg cggcgaatgt gggaattgcc caggcggcgg
     9361 gggatagggg ctgcagacag ttatccacta ttcctgtgga taaccatgtg tattagagtt
     9421 agaaaacacg aggcaagcga gagaatacgc ggcttgcacg cgaattggcg ttaaagacgg
     9481 ctcaatgaaa tatcttttat tttttaactg gttagataaa tgcaatggca gtcactgaac
     9541 aggcatctct tgccataaaa ctgtcatcac tcatcttgac aaatgttaaa aaagccgttg
     9601 ctttggggat aacccggtaa ggccggagtt ttatctcgcc acagagtaaa ttttgctcat
     9661 gattgacagc ggagtttacg ctgtaacaga aatattatgg tgatgaactg tttttttatc
     9721 cagtataatt tgttggcata attaagtacg acgagtaaaa ttacatacct gcccgcccaa
     9781 ctccttcagg tagcgactca tgagtaaacc gttcaaactg aattccgctt ttaaaccttc
     9841 tggcgatcag ccagaggcga ttcgacgtct cgaagagggg ctggaagatg gcctggcgca
     9901 ccagacgtta cttggcgtga ctggctccgg gaaaaccttc accattgcca atgtcattgc
     9961 tgaccttcag cgcccaacca tggtacttgc gcccaacaaa acgctggcgg cccagctgta
    10021 tggcgaaatg aaagagttct tcccggaaaa cgcggtggaa tatttcgttt cctactacga
    10081 ctactatcag ccggaagcct atgtaccgag ttccgacact ttcattgaga aagatgcctc
    10141 ggttaacgaa catattgagc agatgcgttt gtccgccacc aaagcgatgc tggagcggcg
    10201 tgatgtggtt gtggtggcgt ctgtttccgc gatttatggt ctgggcgatc ctgatttata
    10261 tctcaagatg atgctccatc tcacggtcgg tatgattatc gatcagcgcg cgattctgcg
    10321 ccgactggcg gagctgcaat acgctcgtaa tgatcaagca ttccagcgtg gtactttccg
    10381 cgttcgtggc gaggtgatag atatcttccc ggcagaatcg gatgacattg cacttcgcgt
    10441 ggaactgttt gacgaggaag tggaacgatt gtcgttattt gacccgctga ccgggcagat
    10501 tgtttccact attccacgtt ttaccatcta cccgaaaacg cactacgtca caccgcgcga
    10561 gcgcatcgta caggcgatgg aggagatcaa agaagagctg gccgccagac gcaaagtgct
    10621 gttggaaaac aacaaactgc tggaagagca gcggctgacc cagcgtaccc agtttgatct
    10681 ggagatgatg aacgagctgg gctactgttc ggggattgaa aactactcgc gcttcctctc
    10741 cggtcgtgga ccgggtgagc caccgccgac gctgtttgat tacctccctg ctgatgggct
    10801 gctggtggtc gatgaatctc acgtcaccat tccacaaatt ggcggcatgt atcgcggtga
    10861 ccgggcgcgt aaagagacac tggtggagta cggcttccgc ctgccatcag cgctggataa
    10921 ccgtccgctt aagtttgaag agttcgaagc attagcgccg caaaccatct atgtttcggc
    10981 gacgccgggt aattacgagc tggaaaaatc cggcggcgat gtggtggatc aggtggtgcg
    11041 tccaaccgga ttgcttgacc cgattatcga agtgcggccg gtggcgacac aggttgatga
    11101 tcttctttcg gagattcgtc agcgagcggc aattaacgaa cgcgtactgg tcaccacact
    11161 gaccaagcgg atggcggaag atcttaccga atatctcgaa gaacatggcg agcgcgtgcg
    11221 ttatcttcac tcagatatcg acaccgtcga acgtatggag attatccgcg acttgcgtct
    11281 gggtgagttc gacgtattgg tagggatcaa cttactgcgt gaaggtctgg atatgccgga
    11341 agtttcgctg gtggcgatcc tcgacgctga caaagaaggc ttcctgcgtt ccgaacgttc
    11401 gttgatccag accattggtc gtgcggcacg taacgttaac ggtaaagcga ttctctacgg
    11461 cgataagatc accccatcaa tggcgaaagc gattggcgaa accgaacgtc gccgcgagaa
    11521 acagcagaag tacaacgagg aacacggcat tacgccgcaa ggcttgaaca agaaagtggt
    11581 cgatatcctg gcgttggggc agaacattgc taaaaccaaa gcgaagggca gaggaaaatc
    11641 gcgaccgatt gttgagccgg ataatgtgcc gatggatatg tcgcctaaag cgttgcagca
    11701 gaaaatccat gagctggaag ggttgatgat gcaacacgcg cagaatctgg agttcgaaga
    11761 agcggcgcaa attcgtgacc agttgcatca gctgcgtgag ctgtttatcg cggcatcgta
    11821 acaggatagc gaagaagact gatgacaaac ggaaaacagc ctgatgcgct acgcttatca
    11881 ggcctacatt ttctccgcaa tatattgaat ttgcgcggtt tgtaggccgg ataaggcgtt
    11941 cacgccgcat ccggcataaa caacgcgcac tttgtcttca ataggaagcc ggaattttcc
    12001 ttcggatttc cgttaaccta aagcctgtaa cgccttttcc agcgcgttat gtaacaactg
    12061 gcggtcatga cgatacggaa tatcgctggc ctccagtacc tcctggatta caatccgctc
    12121 tttcaccgcc gagacatcca cttttggccc gacgatgacc gcatcaatga cctttttacc
    12181 aacatactgc tccataattg ccagcttgct ttccagcttc aaattagccg caggtaaact
    12241 caactcacgc cccagattgc cgatataaac catcggcgct ggcgtgcggc gtaatgcctg
    12301 ggcgatttcc ttcagcagca gaattggcat caggctggta taaaaactgc caggcccaat
    12361 aatgatgaga tccgcttcat tgatagcgtg aaccgcctca cgcgttgcgg gtacattagg
    12421 cgttaacaat aactcttgaa tcggcgtagt taactggtcg atattgacct cgccgtaaac
    12481 ttcatgcccc tgatcgtcaa tcgccatcag atcaacagga tgctctgaca ttggaatcaa
    12541 atgcgtatcc actttcagca gattacgaat taaattgatg gcttccagag gccgcacgct
    12601 aaggtgatcc agcgccttta acatcaagtt tccgagatta tgaccggaaa gttcgccatt
    12661 gccaccaaaa cggtattcaa acatcgcgga ggcgacgctc ggttccgtta tcagctggtt
    12721 gaggcagttg cgcatatcgc cccaggcaat gccgccttct gaacggcgaa tacgccccgt
    12781 cgagccacca ttatcggtgg tggtgacgat acccgttaaa cgagaaccca aagacgaaag
    12841 tgatgagaga acgcgtccca gtccatgccc tccgccgaga gcaacgacac gatcaagatc
    12901 agccagcgta cgattgcgca tatagtttcc tggagtcaga ttatccgcgc tacagtagcg
    12961 caaattgcgg ggaaacagca attaacctgc caatattgag gatacaaact attttcttct
    13021 acctctaaag gacgatgcac gctatgcctc cctgatgatg tatatcaaag taaaaccgcc
    13081 attttccctt attctgtagc gaaatagcac gatcatgacg ctatatacat gattacatag
    13141 cgaaagtgtg gatggtaaaa atctcattta cacgctagta tcggcataac cactaaacac
    13201 tctagcctct gcacctgggt caactgatac ggtgctttgg ccgtgacaat gctcgtaaag
    13261 attgccacca gggcgaagga agaaatgact tcgcctcccg tatctggaaa ggtgtacatg
    13321 gcttcacaac tgactgatgc atttgcgcgt aagttttact acttgcgcct gtcgattacc
    13381 gatgtgtgta actttcgttg cacctactgc ctgccggatg gctacaaacc gagcggcgtc
    13441 accaataaag gctttcttac cgtcgatgaa attcgccggg ttacgcgcgc cttcgccagt
    13501 ctgggcaccg aaaaagtgcg cctgacaggc ggtgagccgt ctttacgccg cgactttacc
    13561 gatatcatcg ccgctgtgcg ggaaaacgac gctatccgcc agattgcggt caccaccaat
    13621 ggttaccgtc tggaacgcga tgtggcgaac tggcgcgatg cgggacttac tggcattaac
    13681 gtcagtgtcg acagtctgga cgcccgccag tttcacgcta ttaccgggca ggataaattc
    13741 aaccaggtca tggcggggat tgatgctgca tttgaggtcg gttttgagaa ggtcaaagtc
    13801 aataccgtgc tgatgcgtga tgttaatcat caccagctcg acacctttct gaactggatc
    13861 cagcatcgcc ctatccagct gcgtttcatc gaactgatgg aaacgggcga gggcagcgag
    13921 ctcttccgta agcatcacat ctctggtcag gttctgcgtg acgagctact gcgtcgcggc
    13981 tggatccacc aattacgtca acgcagcgac ggtcccgcgc aagtcttttg ccatccagat
    14041 tacgccggag agattggcct tatcatgccg tatgaaaaag acttctgcgc cacttgcaac
    14101 cgcctgcgcg tttcctccat tggtaaactc catctctgcc tgtttggtga aggcggcgtt
    14161 aacctgcgcg atctgctgga agacgatacc cagcaacagg cgctggaagc gcgtatttca
    14221 gcggcgctgc gggagaagaa acagacccat ttcctgcatc aaaacaacac cggtattacg
    14281 caaaacttat cgtacattgg cggctaaaac gtcaaaagga gagatcagat gagtcaggta
    14341 agcactgaat ttatcccgac ccgtattgct attcttacgg tttctaatcg tcgcggtgaa
    14401 gaagacgata cctccggtca ctatctgcgc gattcggcgc aagaagcggg ccatcacgtt
    14461 gtcgataaag ccattgtgaa agaaaaccgc tacgctattc gcgctcaggt atctgcgtgg
    14521 atcgccagcg acgatgtaca agtggtattg attacgggtg gtactggcct gacggaaggt
    14581 gatcaggctc ccgaagcatt gctgccgttg ttcgaccgtg aagttgaagg ttttggtgaa
    14641 gtgttccgga tgttgtcttt tgaagagatt ggcacttcca cgttgcaatc tcgtgcggta
    14701 gcgggcgtcg ccaacaaaac gctgattttc gccatgccgg gttcgaccaa agcgtgccgt
    14761 accgcatggg aaaatatcat cgcgccgcag ctggatgccc gtacgcgtcc gtgtaatttc
    14821 catccacatt tgaagaaata agtatgtcgc aactgaccca tatcaacgcc gctggcgaag
    14881 cgcacatggt ggatgtctcc gccaaagcgg aaaccgtgcg tgaagcgcgg gcggaagcct
    14941 ttgtcaccat gcgcagcgag acgctggcga tgattattga tggtcgccac cacaaaggcg
    15001 acgtatttgc cactgcgcgt attgccggta ttcaggcggc aaaacgcacc tgggatctga
    15061 tcccgctctg tcatccgctg atgctcagca aagttgaagt caatttacag gccgagccgg
    15121 agcacaatcg ggtgcgtata gaaaccttat gccgcctgac cgggaaaacc ggtgtcgaaa
    15181 tggaagcatt aaccgcggcc tccgtggcgg cgctgaccat ttatgacatg tgcaaagcgg
    15241 tgcaaaaaga tatggtgatt ggtccggtac gtttgctggc gaagagcggc ggcaagtcgg
    15301 gtgactttaa ggtggaagcg gatgattaaa gttctttttt tcgcccaggt gcgcgagttg
    15361 gtgggaacag atgcaaccga agtggctgcg gatttcccaa ctgttgaagc gttacgccag
    15421 cacatggctg cgcagagcga tcgctgggcg ctggcgctgg aagatggcaa attactggct
    15481 gccgtcaacc agacgctggt gagttttgac catccgctga ctgacggcga cgaagtagct
    15541 ttcttcccgc cggtaaccgg aggttaagat ggcagaaacc aaaattgttg ttggtccgca
    15601 gccgttcagc gtaggagaag agtacccgtg gctggcggag cgtgacgaag acggtgcggt
    15661 agtcaccttt actggtaagg tgcgcaacca taacctgggc gacagcgtca aagcattaac
    15721 cctcgaacac tatccgggga tgactgaaaa agcactggca gaaattgttg atgaagcgcg
    15781 taaccgctgg ccgctggggc gcgtcactgt gattcaccgc atcggggaat tatggccggg
    15841 cgatgaaatc gtttttgtcg gtgtcaccag tgcgcatcgc agcagtgcgt ttgaagccgg
    15901 gcagtttatt atggattatc tcaaaacccg cgcaccgttc tggaagcgcg aagccacgcc
    15961 ggaaggcgac cgctgggttg aagcccggga gagcgatcag caggcggcaa aacgctggta
    16021 gttttttgtt agccggataa ggcaccgctg cgtccggcaa tttcaccgct cagtacatac
    16081 gtttacgctg cgtcgatgca cagcctcatc attttgcagt atccttaaga tattccttat
    16141 atcttcagga gatcgtcatg gacagattcc cacgttctga ttcaatcgta caaccccggg
    16201 ctggcttgca aacttatatg gcgcaagtct atggttggat gaccgttggc ttgttgctga
    16261 ccgcatttgt tgcctggtat gcggctaatt ccgcggccgt gatggagctg ttgttcacta
    16321 accgtgtctt tttaatcggt ctgatcatcg cgcaattagc attggttatt gtgttatcag
    16381 cgatgattca aaagctgagc gcaggtgtaa cgacgatgct ctttatgctt tattcggcgc
    16441 tgacgggtct tacgctttcc agtatattca ttgtctatac cgctgcttct atcgccagta
    16501 ctttcgtcgt tactgccggg atgttcggcg caatgagcct gtacggttac accacgaagc
    16561 gcgatttaag tggctttggc aatatgctgt ttatggcgtt aatcggcatt gtgctggcat
    16621 cgctggtcaa cttctggttg aaaagcgaag cattgatgtg ggcagttacc tacatcggcg
    16681 tgattgtctt tgtcggattg acggcgtatg acacgcagaa actgaaaaat atgggtgagc
    16741 agattgatac ccgcgacacg tcgaacctgc gcaaatattc cattcttggc gcgttaacct
    16801 tgtatctgga cttcatcaac ctgttcctga tgttgttgcg gatcttcggc aaccgccgtt
    16861 aaattctctt cttcctgctg atcccggcct ggagccggga tttttttcgg attttataaa
    16921 gactattccg ctcatttaca tgagaactga atcgcggcaa atgccagact atgtccacta
    16981 cgtaatttca gggacagata acgcatttga acagacaatc cactgctact caattacagc
    17041 aaaaaagtgc ttcgctcagc aaaatgctgc ttctcatttg gtggttaaca gcagctggag
    17101 tatgggctac ggcaggctgt gcctggatat ttcagcattt gcattgggtt acgtcacaaa
    17161 agtctccagg tgcgtttgtc ttcgctttgg ctgcgctgat aataggtggt ctctggttaa
    17221 acaacgccct gaaaagactg aatgtatcag gcgcatgtct ggtctatctg ctaataatcc
    17281 ttcttgttgg ctttctcttc gccagccttt ataccttttc gagcatagcg acggtaatgg
    17341 gaattacggg agggatgttt gccgcaatgg cgctgatttg ctcttgtagt aatagggtta
    17401 tcccaccagt cagacagtta tattcatata ttttctgtgg tttgagtatt gcttttgttg
    17461 ttaacctgat ccttaccagc agtttttccg tgtggttagc gagtattttg actgtcttta
    17521 tctggggcat tacggctgcc tgtgaagcaa caacactgga agatttgatc cgtgttgccg
    17581 acacctctga aatttcaggt tctcttcgct gtatcgttcc tggggcaata acactctatt
    17641 ttagtatttt aagcgtcctc tttagaatta ctgtaacagt tcttgaattt attaatggtt
    17701 tagtgtggta gtgcgaattg acgagtttta ttgcgttaat tggaatagat aatgatattt
    17761 aacttaagga tataaaaata atgaattcgc gctcagcaaa taacataact aatgattttc
    17821 agcacgaagc caggatatta aacagcatat ggctcattac cgctttaggt ttggtggcaa
    17881 ccgcaggact ggcctgggga gctaagtatc tcgaaattac ggcaaccaaa tatgattcac
    17941 caacaatgta tgtcgccata gggttattat tagtttgtat gtatggctta agtaaggata
    18001 tcaacaagat aaatgccgtc atcgcaggcg taatatatct gtttttactc tctttggtgg
    18061 cgattgtcgt tgcaagttta gctcctgtac ctgccattat cgtcgtgttc agcactgcgg
    18121 gcgcgatgtt tttaatcagt atgctggccg gtttattatt taatgttgat cctggttctc
    18181 accgttttat cattatgatg acgttgacag ggttggccct ggtgatcatc atgaatgccg
    18241 cattaatgag tgaacgaccc gtttgggtaa taagttgctt aatgattgtg ttatggtcag
    18301 gcattatctc gcatggacga aataagctcc ttgaattggc ggggaaatgc catagtgaag
    18361 agttgtggag tccggttcgt tgcgctgtta caggtgcatt aacactctat tactatttta
    18421 tcggcttctt tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc
    18481 gtttttttca ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg
    18541 cttcattttt cgcccgtagc ttcttcgcct ggctttccag tagcaggtaa cagatcagcg
    18601 ccagcagcag cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc
    18661 tccctttcga ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga
    18721 tccccgccgg aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta
    18781 caaagaaata gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc
    18841 agttaacgct ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct
    18901 tttgcccttt gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg
    18961 cgataatcat cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt
    19021 gatccggcaa ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca
    19081 gccagttggt ggtaatactg agcgagaaaa tccgcgtaat agtgctgccc ggtaacccta
    19141 gtcgggagta caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt
    19201 tgaaggcgta gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc
    19261 agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc
    19321 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    19381 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    19441 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    19501 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    19561 ctgcgccaga cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    19621 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttcg gcagcatggt
    19681 ctcatcgacc tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg
    19741 gttaaaatga cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    19801 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcca ctttgccgtg
    19861 gagcgggcgg cggcggtact caaatacctg aacgccgcct ttaaccagat agttatacag
    19921 caagcgcgca ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    19981 ccgcaccccg cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    20041 gtaggcgttg gcgataatga cttctcgccg cgcctgagtg agcattttca gatagtgacg
    20101 ttcaatatca tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc
    20161 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    20221 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    20281 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    20341 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    20401 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc gaaataccac
    20461 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    20521 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    20581 tttgccgacg tcatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    20641 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    20701 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    20761 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    20821 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    20881 ccgtggacgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    20941 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    21001 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    21061 tgtacgcaca tcacatgaat cgcttttccg gtcatcggtg gcacaatgcg gcagtagagc
    21121 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    21181 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    21241 gcaaaatcgc tccacatcgt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    21301 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    21361 gcgctgacgg tacgcaccgc gtcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    21421 gtaaaacctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    21481 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    21541 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    21601 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaact ggtctaagtt
    21661 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    21721 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    21781 tgttggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    21841 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgtg
    21901 tactggaaaa caactttttg ctggcaattg cagggctggc aatccttgcc ggcccggcgg
    21961 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    22021 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    22081 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    22141 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    22201 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    22261 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    22321 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    22381 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    22441 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    22501 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    22561 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    22621 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    22681 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    22741 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    22801 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    22861 agcagcacat gagtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    22921 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    22981 aacgggaaca gaatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgcggt
    23041 tcgcgcagca gcgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    23101 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    23161 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    23221 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    23281 caccgcgggc atactgtcga tctgaaaaat aaagccagag agcataatcg acggcaggaa
    23341 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    23401 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag
    23461 cgacccgcga tacggcacgc cgagaataaa caccgacacc agcatacaca gcagcatcgc
    23521 cagcatcccg agaaagtaat aggggatcag cttacacagc agcagctccg tgcgggtaat
    23581 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    23641 ggtgagaatc gcgccgatga ccgtcatgat gatggtcacc gcaccgggga taatgaagtg
    23701 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    23761 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    23821 cccctgcaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    23881 ggtggcgttg gcgcgctcca tctgttcggc gaaatccacc ggaataacca ccagaccgcg
    23941 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    24001 gatgtagggc gaaccggtca tagtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    24061 cagtaaaatc ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa
    24121 cagcagcaac agcgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    24181 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    24241 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    24301 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtgccg ctggcgatta
    24361 atttcccacg gtacaccagg ccaatgcggt cgcaatattc cgcttcatcc ataaagtggg
    24421 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    24481 ggcgggtgag aggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    24541 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    24601 cgtgtgaggc aatacttttc aggccaaatg cctcgctcat acgggagatt ttttcgttct
    24661 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    24721 ggttgccgta gagcgaaaat ttttgcgcca tatagccgag atgctggcgc gctttaccgg
    24781 aactctcttt cagatccatc cccagcacca gcgcctggcc agaggtcggc accagcaaac
    24841 cgcacatcat cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa
    24901 tctccccacg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    24961 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgcaata
    25021 ttgcacccag cggcgattct gaggttccgg caccgcccag caaatcaata aacgcatctt
    25081 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    25141 cgtctggtgt ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    25201 cgctgacctg cggcagtttc aaggcgcgtt gcaacagttt gcggttgccc tcgtgtggac
    25261 tggtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tctccctgat
    25321 acagcaactc gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    25381 acgaggtact ccagaggatt aacatccctt cgcccgccag ctcatgcacc atctgccaca
    25441 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    25501 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    25561 ccgccaggcg tccggtaaac gggccaagag atgtaaactc cagcaggcga gcaaaagttt
    25621 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    25681 ccgtcagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    25741 cgccgtcgtt tttgatcgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    25801 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    25861 ccgtcacata accggcgtga atggtacagt cgagcggcgc gacggcgggc ttgtccatgc
    25921 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    25981 tcaccgaatt gtaccgtcac tggcattccc tggcgtaacg catcatcggc gtcggtcacc
    26041 acaatacgca ggcgatagac gaggtcggta cgcagatccg gcgtttcgac ggttttcggg
    26101 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatagggctt gtccgggcga
    26161 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca
    26221 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    26281 ttgagaaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggtgctatc
    26341 aacgttgagt cttgtaaatt cagctccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    26401 ctggctttcg cctgagcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    26461 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    26521 tcatttgccg aaatagtgcg gcttttccac aacccttgct ggcggttata gaagttctgc
    26581 gcatagtcat aggcggcttg cgcctgtttc accgcagcgg cggcctgagc gatttcttca
    26641 tcgcgatacc cggcaagcat caggtcatac tgcgcctgtg ccaccgagac gcccgctttc
    26701 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    26761 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    26821 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    26881 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc
    26941 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    27001 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    27061 cggtacgtaa caaaatggtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    27121 catgggtgtg aaggatcatg cgggtatcgt tggcgtcgca gccggtccag gcggcaatca
    27181 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    27241 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    27301 tgagcagctt aatcatgttc ctgcaggcgc gaaggatcag ttcacggata gctgcgcgat
    27361 caggctgtgg ttgtgcgaac aagcattcgg cttcttcggc gtgcggacga aactgctcgc
    27421 caatgaaatc ggcaatccat tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    27481 aataggtgat ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt
    27541 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    27601 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    27661 attaagatta tgactccata ggggagttgt ccagtatggc taagaatttt ggctacgcca
    27721 gtcacaggga taatttatgc gctgtgtcac aaaaactgct acactccgct ccctcatgac
    27781 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    27841 ggggcggttc ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg
    27901 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    27961 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggtaccggta aaacagcggg
    28021 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    28081 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gctgcgcaga ttggcgaaaa
    28141 cgtccgtgat tacagcaaat atttgaacat tcgttcgctg gtggtgtttg gcggtgtcag
    28201 cattaacccg caaatgatga agctgcgcgg cggcgttgat gttctggtgg caacaccggg
    28261 acgtttgctg gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt
    28321 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    28381 aacaaaactg ccagcgaaac gccagaacct gctgttctct gcgaccttct ctgacgatat
    28441 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    28501 taccgcgtct gatcaggtga cacagcacgt tcactttgtc gataagaaac gtaaacggga
    28561 actgttgtcg cacatgattg gcaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    28621 caaacatggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gcagtgcggc
    28681 gatccacggt aataaatcgc aaggtgcgcg tacccgtgcg ctggccgatt ttaaatcggg
    28741 tgacattcgc gtactggtgg caactgacat cgctgcgcgc ggtctggata tcgaagagtt
    28801 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg
    28861 gcgtaccggt cgtgccgcag ccaccggaga agcgttgtcg ttggtgtgtg ttgatgaaca
    28921 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    28981 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    29041 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    29101 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    29161 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaaccta ccgctgcgca
    29221 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    29281 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    29341 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    29401 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    29461 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    29521 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    29581 gcgcggttgc cagcaagagc gcacgcagtt ctggatgctg ttcgaattta gcgcgaagtg
    29641 cttttcgcat cacctgttct ttgaccgact cccagttttt acgcagaggc ttagaacgat
    29701 cgcgccccat gcgtgcggcg accatagggg aagaaacccg acggatctct tcgcggtatt
    29761 tttcatcaag gaatttttgt gcctgaaaat agtgttctga ggtgggccag gttttcccgt
    29821 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    29881 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    29941 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    30001 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    30061 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    30121 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    30181 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    30241 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    30301 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    30361 aagatcattc ccgatcttaa attcactgcc gcttttgggc gtgggcgcta cgtttgcccg
    30421 cgtaatctga cggcgctcgc cagcactgag cccacgcaac aggatctgct ggcgtttctt
    30481 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    30541 gatctcgaca cttataaatg ggatggtctg cgcgatcata ctgatatcgc tatagatgac
    30601 gatctctggc gtcgtttaag taccgacaaa gccagctgcc tcaaccgcaa ctgttactac
    30661 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    30721 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactacc tgacccgaaa
    30781 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    30841 gagatgagtg ccgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    30901 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    30961 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    31021 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    31081 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    31141 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    31201 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    31261 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    31321 cgggaagagc gcgaagggca gctgcatctc tggtttcact gcgtggggat acgtgtcagc
    31381 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    31441 ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga gcgggctgaa agagaaagcg
    31501 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    31561 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    31621 gcggcctttt tccgtgagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    31681 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgctg
    31741 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    31801 aacggtgagc atagtgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    31861 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    31921 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    31981 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    32041 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    32101 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    32161 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    32221 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    32281 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgcccatat gctcaatggt
    32341 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tacgtatcaa aggggaaggg
    32401 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    32461 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    32521 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    32581 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    32641 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    32701 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    32761 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag
    32821 gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    32881 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    32941 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    33001 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    33061 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    33121 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    33181 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    33241 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    33301 aaaaaacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    33361 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    33421 aagaagcgaa attagtggcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc
    33481 atggtattgg catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta
    33541 accatcatgc caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg
    33601 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    33661 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    33721 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    33781 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    33841 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    33901 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    33961 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    34021 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    34081 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    34141 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    34201 gcgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
    34261 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga
    34321 ttccattgga tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt
    34381 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaag cccgtccagt
    34441 aacggacggg caaacaaggg taatatagga tcaatgaggg ttagagcata tgcgtctgtc
    34501 ggcaaacaga cagggaaata cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg
    34561 gttcggatt
//
LOCUS       DAFAVF010000034        34437 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFAVF010000034 DAFAVF010000000
VERSION     DAFAVF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.034"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            131..4699
                     /gene="sslE"
                     /locus_tag="LGK42_001630"
     CDS             131..4699
                     /gene="sslE"
                     /locus_tag="LGK42_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBK2657891.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIIL
                     SEGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLS
                     NGATLDEGEQVVNLPNAFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKA
                     SMTVGTNLDTVYFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNG
                     FEYVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTID
                     SKTGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKE
                     KIFAAFPGLKECTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQ
                     AADLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKND
                     ELGFKTFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNY
                     MEKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQST
                     GLWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTV
                     KFKVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAF
                     VYTTPKKNLNASNYTGGLEQFANDLDTFARSMNDFYGRDETSGKHRMFTYKALTGHKH
                     RFTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPG
                     ATEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQL
                     KEWAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNY
                     CAESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYN
                     TLASLNLPKPKQGPETINKVTEYSMPAE"
     gene            4839..5648
                     /gene="pppA"
                     /locus_tag="LGK42_001631"
     CDS             4839..5648
                     /gene="pppA"
                     /locus_tag="LGK42_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HBK2657892.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVIIKKGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5714..6124
                     /gene="gspS2"
                     /locus_tag="LGK42_001632"
     CDS             5714..6124
                     /gene="gspS2"
                     /locus_tag="LGK42_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBK2657893.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLISYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6142..7101
                     /gene="gspC"
                     /locus_tag="LGK42_001633"
     CDS             6142..7101
                     /gene="gspC"
                     /locus_tag="LGK42_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBK2657894.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            7131..9191
                     /gene="gspD"
                     /locus_tag="LGK42_001634"
     CDS             7131..9191
                     /gene="gspD"
                     /locus_tag="LGK42_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBK2657895.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9191..10684
                     /gene="gspE"
                     /locus_tag="LGK42_001635"
     CDS             9191..10684
                     /gene="gspE"
                     /locus_tag="LGK42_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBK2657896.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10684..11907
                     /gene="gspF"
                     /locus_tag="LGK42_001636"
     CDS             10684..11907
                     /gene="gspF"
                     /locus_tag="LGK42_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBK2657897.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11924..12379
                     /gene="gspG"
                     /locus_tag="LGK42_001637"
     CDS             11924..12379
                     /gene="gspG"
                     /locus_tag="LGK42_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBK2657898.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12383..12946
                     /gene="gspH"
                     /locus_tag="LGK42_001638"
     CDS             12383..12946
                     /gene="gspH"
                     /locus_tag="LGK42_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBK2657899.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPAAPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            12943..13314
                     /gene="gspI"
                     /locus_tag="LGK42_001639"
     CDS             12943..13314
                     /gene="gspI"
                     /locus_tag="LGK42_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBK2657900.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13311..13916
                     /gene="gspJ"
                     /locus_tag="LGK42_001640"
     CDS             13311..13916
                     /gene="gspJ"
                     /locus_tag="LGK42_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBK2657901.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            13913..14890
                     /gene="gspK"
                     /locus_tag="LGK42_001641"
     CDS             13913..14890
                     /gene="gspK"
                     /locus_tag="LGK42_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBK2657902.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLTQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14887..16065
                     /gene="gspL"
                     /locus_tag="LGK42_001642"
     CDS             14887..16065
                     /gene="gspL"
                     /locus_tag="LGK42_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBK2657903.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            16067..16603
                     /gene="yghD"
                     /locus_tag="LGK42_001643"
     CDS             16067..16603
                     /gene="yghD"
                     /locus_tag="LGK42_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HBK2657904.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(16752..16827)
                     /locus_tag="LGK42_001644"
     tRNA            complement(16752..16827)
                     /locus_tag="LGK42_001644"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16792..16794),aa:Phe,seq:gaa)
     gene            complement(16933..17640)
                     /locus_tag="LGK42_001645"
     CDS             complement(16933..17640)
                     /locus_tag="LGK42_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK2657905.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            18038..20173
                     /gene="speC"
                     /locus_tag="LGK42_001646"
     CDS             18038..20173
                     /gene="speC"
                     /locus_tag="LGK42_001646"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBK2657906.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20223..21479)
                     /gene="nupG"
                     /locus_tag="LGK42_001647"
     CDS             complement(20223..21479)
                     /gene="nupG"
                     /locus_tag="LGK42_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBK2657907.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21681..22760)
                     /gene="mltC"
                     /locus_tag="LGK42_001648"
     CDS             complement(21681..22760)
                     /gene="mltC"
                     /locus_tag="LGK42_001648"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBK2657908.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22825..23100)
                     /locus_tag="LGK42_001649"
     CDS             complement(22825..23100)
                     /locus_tag="LGK42_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBK2657909.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(23128..24180)
                     /gene="mutY"
                     /locus_tag="LGK42_001650"
     CDS             complement(23128..24180)
                     /gene="mutY"
                     /locus_tag="LGK42_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK2657910.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24341..25060
                     /gene="trmB"
                     /locus_tag="LGK42_001651"
     CDS             24341..25060
                     /gene="trmB"
                     /locus_tag="LGK42_001651"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK2657911.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            25060..25386
                     /locus_tag="LGK42_001652"
     CDS             25060..25386
                     /locus_tag="LGK42_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBK2657912.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            25570..26289
                     /locus_tag="LGK42_001653"
     CDS             25570..26289
                     /locus_tag="LGK42_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBK2657913.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26465..27511
                     /gene="ansB"
                     /locus_tag="LGK42_001654"
     CDS             26465..27511
                     /gene="ansB"
                     /locus_tag="LGK42_001654"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBK2657914.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27628..28635
                     /locus_tag="LGK42_001655"
     CDS             27628..28635
                     /locus_tag="LGK42_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBK2657915.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(28790..29926)
                     /gene="hemW"
                     /locus_tag="LGK42_001656"
     CDS             complement(28790..29926)
                     /gene="hemW"
                     /locus_tag="LGK42_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBK2657916.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(29919..30512)
                     /locus_tag="LGK42_001657"
     CDS             complement(29919..30512)
                     /locus_tag="LGK42_001657"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBK2657917.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(30520..30810)
                     /locus_tag="LGK42_001658"
     CDS             complement(30520..30810)
                     /locus_tag="LGK42_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBK2657918.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(30807..31373)
                     /gene="yggT"
                     /locus_tag="LGK42_001659"
     CDS             complement(30807..31373)
                     /gene="yggT"
                     /locus_tag="LGK42_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBK2657919.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(31391..32095)
                     /gene="yggS"
                     /locus_tag="LGK42_001660"
     CDS             complement(31391..32095)
                     /gene="yggS"
                     /locus_tag="LGK42_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBK2657920.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            32113..33093
                     /locus_tag="LGK42_001661"
     CDS             32113..33093
                     /locus_tag="LGK42_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBK2657921.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            33369..34349
                     /locus_tag="LGK42_001662"
     CDS             33369..34349
                     /locus_tag="LGK42_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HBK2657922.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT         8454 a         8622 c         9502 g         7859 t
ORIGIN      
        1 aaatttcacc tgttttctat caatgatgtc gttttcttaa gaatggagga aattattaaa
       61 gaagataaag cataaaacta tttctcccag ttacgaattt tttaacattg ttttgtcact
      121 tgcgttatta atgaataaga aatttaaata taagaaatcg cttttagcgg ctattttgag
      181 tgcaaccctg ttagccggtt gtgatggcgg tggctccgga tcttcctccg atacgccgcc
      241 tgtagattct ggaacagggt ctttgccgga agtgaaacct gatccaacac caaacccgga
      301 gccgacgcct gagccaacgc cggacccaga acctacgccg gaaccgacac ctgatcctga
      361 gccaacacca gaaccggagc cagaacctgt tcctacgaaa acgggttatc tgactctggg
      421 cggaagcctg cgggtaactg gtgatatcac ctgtaatgat gaatccagcg atggctttac
      481 ctttacacca ggcgacaaag tcacctgtgt ggcagggaac aacacgacaa ttgctacctt
      541 cgacacccag tcagaagctg cgcgtagcct gcgtgcggtt gaaaaagtgt cgtttagtct
      601 tgaggacgcg caagaactgg cgggttccga caacaagaaa agcaatgcgc tctcgctggt
      661 cacctccatg aacagttgcc cggcgaatac agaacaggtg tgcctggagt tctcctcggt
      721 gatcgagagt aaacgtttcg actcgctgta taagcaaatc gatctggcac cggaagagtt
      781 caaaaagctg gtcaatgaag aggtggaaaa caatgccgcg actgataaag cgccatccac
      841 tcatacttca ccggtagtcc ccgtcacgac tccgggaaca aaaccggatc tgaacgcctc
      901 cttcgtgtcg gctaacgcgg aacagtttta tcagtatcaa ccttcggaaa tcattctctc
      961 agaaggtcgc ctggtggata gccagggata tggtgttgct ggcgtcaact actacaccaa
     1021 ttcaggccgt ggcgtgacag gggaaaatgg tgaattttct tttagctggg gcgaaaccat
     1081 ctcctttggt atcgatacct ttgaactggg ttcagtgcgc ggcaataagt cgaccattgc
     1141 gctgactgaa ctgggtgatg aagttcgcgg ggcgaatatc gatcagctta ttcatcgcta
     1201 ttcgacgacc gggcaaaata atacccgtgt tgttccggat gatgtacgca aggtctttgc
     1261 cgaatatccc aacgtgatca acgagattat caatctctcg ttatccaacg gtgcgacgct
     1321 ggatgaaggt gagcaagttg ttaatctgcc aaacgcattt atcgagcagt ttaagacggg
     1381 tcaggccaaa gagatcgata ccgcgatttg tgcgaaaacc gacggttgta acgaggctcg
     1441 ctggttctcg ctgacgacgc gcaatgttaa tgacggccag attcagggcg ttatcaacaa
     1501 gctgtggggc gtggatacga actacaaatc tgtcagcaag ttccatgtat tccatgactc
     1561 taccaacttc tatggcagca cgggtaatgc gcgcggtcag gcggtggtga atatctccaa
     1621 cgcggccttc ccgattctga tggcgcgtaa tgataaaaac tactggctgg cgtttggcga
     1681 aaaacgcgcc tgggataaaa atgagctggc gtacattacg gaagcgcctt ctcttgttga
     1741 gccggaaaac gttacgcgcg ataccgccac tttcaacctg ccgtttattt cgctggggca
     1801 agtcggtgag ggcaaactga tggttatcgg taacccacac tacaacagca ttttgcgttg
     1861 cccgaacggt tacagctgga acgggggcgt taataaagat gggcagtgta cgctcaacag
     1921 cgacccggat gacatgaaga acttcatgga gaacgtgctg cgctatctgt ccgacgataa
     1981 atggacgccg gacgcgaaag ccagcatgac cgtaggcacc aacctggata ctgtctattt
     2041 taaacgtcat ggtcaggtta caggaaacag cgctgcgttc gacttccatc cggattttgc
     2101 gggcatctct gttgagcatt taagtagcta tggcgatctc gatccgcagg aaatgccgct
     2161 gctgatcctt aacggctttg aatatgtgac tcaggtgggt aacgatcctt atgcaatccc
     2221 gctgcgtgca gataccagca aaccgaagct gactcagcag gatgtgaccg atctgatcgc
     2281 ctatctgaac aaaggtggat cggtgctgat catggaaaac gtgatgagca atcttaagga
     2341 agagagcgcg tctggttttg tgcgtctgtt ggatgccgca ggtctgtcga tggcactgaa
     2401 caagtcggta gtaaataacg atccgcaagg gtatccgaac cgcgttcgtc agcagcgcgc
     2461 aacgggcatt tgggtctatg aacgttatcc tgccgtagat ggtgcgctgc cgtacaccat
     2521 cgatagtaag acaggggaag ttaagtggaa atatcaggta gaaaacaaac ctgatgacaa
     2581 accgaagctg gaagttgcca gctggctgga agatgtagat ggcaaacagg aaacgcgtta
     2641 tgcctttatt gatgaggccg atcataaaac agaggattct ctgaaggctg cgaaggagaa
     2701 aattttcgcc gcgttcccgg ggctgaaaga gtgtactaat ccggcatatc actatgaggt
     2761 caactgtctg gaatatcgtc ctggcacggg ggttccggtt actggtggca tgtatgttcc
     2821 acagtatacg caactaagcc ttaacgccga cactgcaaaa gcgatggtgc aggctgcgga
     2881 tttaggcacc aacattcagc gtctgtatca gcatgagctc tacttccgga ccaatggtcg
     2941 caaaggtgag cgtctgagca gcgtcgatct ggaacgtctg taccagaaca tgtcggtctg
     3001 gctgtggaac gatacgagct atcgttatga agaaggcaaa aatgacgagc tgggctttaa
     3061 aacgttcacc gagttcctga actgctacgc caatgatgcc tatgcaggcg gcaccaagtg
     3121 ttctgcagat ctgaaaaaat cgctggtcga taacaacatg atctacggtg acggtagcag
     3181 caaagcgggc atgatgaacc cgagctaccc gctcaactat atggaaaaac cgctgacacg
     3241 cctgatgctg ggccgttcct ggtgggatct gaacatcaaa gttgatgtcg agaagtatcc
     3301 gggagcagta tcggaagagg gacagaacgt tactgaaacc atcagcctgt actcgaatcc
     3361 gaccaaatgg tttgcaggta acatgcagtc aactggcctg tgggcaccgg ctcagaaaga
     3421 ggtcaccatt aagtccaatg cgaacgttcc tgtgaccgtc accgtggcgc tggctgacga
     3481 cctgaccgga cgtgagaagc atgaagttgc gctgaaccgt ccgccaagag tgactaaaac
     3541 gtactctctg gacgctagcg gtacggtgaa gttcaaggtg ccttacggtg gcctgattta
     3601 tatcaagggc aatagctcta ccaatgaatc tgccagcttc acctttactg gcgtggtaaa
     3661 agcaccgttc tataaagacg gcgcatggaa aaacgatctg aactcaccgg ctccgctggg
     3721 tgagctggaa tcagacgctt tcgtctatac cacaccgaag aagaacctga atgccagcaa
     3781 ttacactggc ggactggagc aattcgctaa cgatctggac acctttgcca gatcgatgaa
     3841 tgacttctac ggtcgtgatg aaacgagcgg taagcaccgg atgtttacct ataaagcatt
     3901 gacgggacat aaacatcgtt tcacaaacga tgtgcagatc tccatcggtg atgcgcactc
     3961 gggttatccg gtaatgaaca gcagcttctc gacgaacagc accacgctgc cgacgacgcc
     4021 gctgaacgac tggttgatct ggcacgaagt agggcacaac gccgcagaaa caccgctgac
     4081 tgtaccgggt gcaactgaag tcgcgaacaa cgtgctggcg ctgtacatgc aggatcgcta
     4141 tctcggcaag atgaaccgtg tcgctgacga tattaccgtc gcaccggaat atctggagga
     4201 gagcaacaac caggcatggg cacgcggcgg tgcgggtgac cgtctgctga tgtacgcgca
     4261 gctgaaggaa tgggcagaga aaaactttga tatcaagaaa tggtatccag atggcactcc
     4321 tctgccagag ttttacagcg agcgtgaagg gatgaaaggc tggaacctgt tccagttgat
     4381 gcaccgtaaa gcgcgcggcg atgacgttgg tgacaagacc tttggtggca agaattactg
     4441 tgctgaatcc aacggtaacg ctgctgacac gctgatgctg tgtgcatcct gggtcgctca
     4501 gacggatctt tcggagttct ttaagaaatg gaatccgggt gcgaatgctt accagctgcc
     4561 gggggcgagc gagatgagct tcgagggcgg tgtgagccag tcggcgtaca acacgctggc
     4621 gtcactcaat ctgccgaaac cgaagcaagg gccggaaacc attaataagg ttaccgagta
     4681 ttcgatgcct gctgaataac ccggataagg cgttcacgcc gcatccggca tgaaataagg
     4741 cgcaccgggt tgcgccagct tattgggcct ggaggaaagc catatggcat acacctgcag
     4801 gcccgcatcc ggcaagttac aacaaacaac ctttaaccat gctttttgat gtttttcagc
     4861 aataccccgc ggcgatgccc atactggcta ccgtcggagg attgattatc ggcagttttt
     4921 tgaatgtggt gatttggcgt taccccatca tgctgcgcca acaaatggcg gagtttcatg
     4981 gtgaaatgcc gagtgcgcag tcaaaaataa gccttgcgct accgcgttcg cactgcccac
     5041 attgtcagca gaccatccgg atacgtgaca atattccgct gctctcctgg ctgatgctca
     5101 aagggcgctg ccgcgactgt caggcgaaaa tcagcaagcg ttatccgctg gtggagttat
     5161 tgacagcact cgcttttttg ctggcgagtc tggtctggcc ggaaagtgga tgggcgctgg
     5221 cggtgatgat attatccgcc tggctgattg ccgcgagcgt cattgacctc gatcaccaat
     5281 ggctgcccga tgtttttact cagggcgtat tgtggacggg actaattgcg gcatgggcgc
     5341 agcagagccc gctcacgcta caagatgcag tcaccggcgt cctggtgggg tttatcactt
     5401 tttactctct gcgctggata gccggaatag ttctgcgtaa agaagcatta ggcatgggcg
     5461 atgtattatt gttcgctgcg ttaggtagtt gggtggggcc gttgtcactg cccaatgttg
     5521 ctttaatcgc ctcatgctgc ggcctgatat atgccgttat tataaaaaaa ggatcaacca
     5581 cactgccttt tggaccgtgt ttaagtctgg gcggtatagc aacactttat ctacaggcat
     5641 tgttttaatg ataaccacgt cattatcaaa gtgacatttt aactcttatt aataacctta
     5701 gagattattt accatgtcga taaaacagat gccagggagg gtattaatat cgctattgtt
     5761 gagcgttaca ggattattaa gtggctgtgc cagccataat gaaaacgcca gtttgctggc
     5821 gaaaaaacag gcgcaaaata tcagccaaaa cctgccgatt aaatctgcgg gatatacctt
     5881 agtgctggca caaagtagcg gcacgacggt aaaaatgacc attatcagcg aagcgggtac
     5941 tcagaccacg cagacgccag acgccttttt aatcagctat caacgacaaa tgtgcgctga
     6001 cccaacagtg aaattaatgc tcactgaggg aattaattac agcataacga ttaatgatac
     6061 acgtacaggt aaccagtatc agcggaaact ggatcgtacc acctgtggaa tagtcaaagc
     6121 ataacgtcgg gtagatataa attggcgcgg gttgtttttc gtgacgcacg aatttatctc
     6181 attcaatggc tgacaaaaat tcgtcacact cttaaccaga gacaatctct taatacagac
     6241 aaagagcatc tgcgaaaaat tgtacgcggg atgttctggc tgatgctgct tattatttct
     6301 gcaaaagtgg cgcattcact ctggcgctat ttctcctttt ctgcggaata tacggctgtt
     6361 tccccatcgg cgaataaacc gccccgtgcg gatgcaaaaa cgttcgataa aaatgacgtg
     6421 caattaatca gccagcaaaa ctggtttggc aaatatcagc ccgtcgccac gccggtaaaa
     6481 caacccgaac ctgtgcctgt ggcagaaacg cgtcttaatg tggtgctgcg cgggattgcc
     6541 tttggtgcca gacccggcgc ggttattgaa gaaggcggta aacagcaggt ctatttgcag
     6601 ggtgaaacgc ttggctcgca caatgcggtg attgaggaaa tcaatcgcga ccatgtgatg
     6661 ctgcgctatc agggaaaaat agagcgcctg agcctggctg aagaggagcg ttccactgtt
     6721 gccgtgacca acaaaaaagc tgtcagtgac gaagcaaagc aagctgttgc tgagcctgct
     6781 gccagtgcgc cagttgagat cccgactgcc gtgcgtcagg cactggcgaa agatccgcag
     6841 aaaattttta actatatcca gcttacgcct gtgcgtaagg aggggattgt cggttatgca
     6901 gtgaagccgg gggcagatcg ttctctgttc gatgccagcg gtttcaagga aggcgatatc
     6961 gccattgcgc taaatcagca ggatttcact gatccacgag caatgattgc tctgatgcgg
     7021 cagttacctt caatggattc cattcaactt acggttttac gcaagggtgc gcgccacgac
     7081 atttccatcg cgctgcgcta accgcattta atccaggaga atcattcatc gtgttttggc
     7141 gtgatattac gttgtcggtc tggcgtaaga agacaactgg cctcaaaaca aaaaagcgtt
     7201 tactgccgct ggtgctggca gcggcattat gcagttcacc ggtctgggcg gaagaagcca
     7261 ctttcaccgc taattttaaa gataccgacc tgaaatcgtt catcgaaacc gtcggcgcta
     7321 accttaataa aaccatcatt atggggccgg gcgtacaggg gaaagtgagt attcgcacca
     7381 tgaccccgct caatgaacgc cagtattacc agctattcct taacctgctg gaagcacagg
     7441 ggtatgccgt cgtaccgatg gaaaacgacg tgctgaaggt ggtgaaatcc agcgctgcga
     7501 aagtcgagcc gctgccgctg gtcggtgaag gcagcgacaa ctacgcgggc gatgaaatgg
     7561 tcaccaaagt agtgccggta cgtaatgtgt cggtacgcga gctggcaccg attctgcgcc
     7621 agatgatcga cagcgcaggc tcaggcaatg ttgttaatta cgatccctcc aacgtgatta
     7681 tgctcaccgg acgcgcctcc gttgtggagc gcctgacaga agtgatccag cgcgtggatc
     7741 acgcgggtaa tcgcaccgaa gaggtgatcc cgctggataa cgcctcggct tcggaaatcg
     7801 cccgcgtgct ggaaagcctg acgaaaaaca gcggcgagaa ccagcctgcg acgctgaaat
     7861 ctcaaattgt cgccgacgaa cgcaccaaca gtgtgattgt cagtggtgac ccggccacgc
     7921 gggacaaaat gcgccgcctg atccgtcggc tggactcaga aatggaacgc agcggcaaca
     7981 gccaggtgtt ctatctcaaa tacagcaaag ccgaagatct ggtcgatgtg ctgaagcagg
     8041 tcagcggcac gctcacggcg gctaaagaag aggcggaagg cacggtcggt agcgggcgtg
     8101 agattgtatc catcgccgcc agcaaacaca gtaatgccct gattgtaact gcaccgcagg
     8161 acatcatgca gtcgctgcaa agcgtgattg aacaactgga tattcgccgt gctcaggtgc
     8221 acgtcgaggc gttgatcgtg gaagttgccg aaggtagcaa tatcaacttc ggcgtgcagt
     8281 gggcgtcgaa agatgccgga ttaatgcagt ttgctaatgg tacgcagatc cctatcggta
     8341 cgctgggggc agccatttct caggcgaaac cgcagaaagg ctcgacggtg atcagtgaaa
     8401 acggcgctac caccattaat ccggatacca acggcgatct ctccacactc gcccagcttc
     8461 tttctggatt tagcggtacg gcggttggcg tggtgaaagg cgactggatg gcactggtgc
     8521 aggcggttaa aaacgactcc agctcgaacg ttctctccac gccgagcatc actacgctgg
     8581 acaaccagga agccttcttc atggtgggcc aggacgttcc ggtattaacc ggctctaccg
     8641 ttggctccaa taacagcaat cctttcaata cagtagaaag gaaaaaagtc ggcatcatgc
     8701 tgaaagtcac gccgcagatt aacgaaggta acgcggtaca gatggtgatt gagcaggaag
     8761 tctcgaaggt ggaaggacag accagcctcg acgtggtgtt tggtgagcgc aaactgaaaa
     8821 ccaccgtgct ggctaacgat ggtgagctga ttgtgcttgg tggtctgatg gacgatcagg
     8881 cgggagaaag cgtggcgaaa gtgccgctgc tgggcgatat cccattgatt ggtaacctgt
     8941 ttaaatcgac ggcggataaa aaagaaaaac gtaacctgat ggtgtttatc cgtccgacca
     9001 ttctgcgtga cggtatggcg gcagacggcg tgtcgcagcg caaatataac tacatgcgcg
     9061 ccgagcagat ctatcgcgat gagcaaggtt taagcctgat gccgcacacc gcgcagccgg
     9121 tactgccagc gcaaaatcag gctctaccgc cggaagttcg tgcattcctt aatgccggga
     9181 gaacgcgtta atggtgcctg tagcacagga aaccaccgct aacaccgtgc gtctgcccta
     9241 cagtttcagc cgtcggttta gcctggtggc atggtgcgaa gcgtcgctgg agatcctcca
     9301 tgtgcatccg ttgtcgctct ctgttttgca ggagctgcag cgggggctga acgcgccctt
     9361 tacgctgcgg caaatcgacg gggccgaatt tgaacagcgg ctgaatgcgg tctggcagcg
     9421 ggactcttcc gaggctcgcc agctgatgga agatctcggt tctgctgagg acttttttac
     9481 tctcgcggaa gaactgccag aaacggaaga tctgctggaa agtgacgacg atgcgccgat
     9541 catcaaactg atcaacgcca tgctggcaga ggcgattaaa gaaggcgctt cggatatcca
     9601 catcgagacg tttgaaaaga gtctggtgat ccgttttcgc gttgacggca cattacatga
     9661 aatgctgcgc ccggggcgca aactggcctc gctgctggtg tcgcgtatca aggtgatggc
     9721 gcggctggac attgccgaaa agcgcgtgcc gcaggatgga cgtattgcgc tgttgctggg
     9781 cggccgggcg attgacgtgc gtgtctccac catgccttct gcctggggcg agcgcgtggt
     9841 gctgcgactg ctggacaaaa accaggcccg cctgacgctg gagcgtctgg ggcttagcca
     9901 gcaactgacc gcgcagttgc gccagctgtt acacaaaccg cacggcatct ttctggtgac
     9961 ggggccgacg ggttccggca aaagcaccac gctgtacgcc ggattgcagg agctgaacaa
    10021 ccattcgcgc aacattctca cggttgaaga tcccatcgaa tacatgattg aagggatcgg
    10081 tcagacgcag gttaacaccc gcgtcggcat gacctttgcc cgtgggctgc gcgcgatttt
    10141 gcgtcaggac ccggatgtgg tgatggtcgg tgaaattcgc gataccgaaa ccgcagaaat
    10201 cgccgtccag gcttcactta ccggacacct ggtcctttcc acgctgcata ccaacacagc
    10261 ggtgggggcg atcacacgtt tgcaggatat gggcgtggag cctttcctgc tctcttccag
    10321 tctgacgggc gtgatggcgc agcgactggt tcgcacgctg tgtcccgact gccgccagcc
    10381 cgcaccagcc actgacgaag aaaaacgcct gctgggaatt accgacgccc gtaccgtcac
    10441 tctgtaccat ccacagggct gtcccgcctg taatcacaaa ggttttcgcg gacggactgc
    10501 catccatgag ctgatcgtgg tggatgccac attgcgtgat ttgatccacc gtcaggccgg
    10561 ggagctggag ctggaacgtt atgtccgaca acactctgcg ggtatccgca gcaacggcat
    10621 tgagaaagtg ctcgccggag aaacctctct cgatgaagtt ctgcgggtaa ccatggaggc
    10681 gtaatggcac tgttttacta tcaggcgctg gagcgtaatg gtcgcaaaac caaaggtatg
    10741 attgaggcgg attccgcgcg tcatgcccgc cagttgttgc gcggtaaaga gcttatcccc
    10801 gtgcatattg aagcccggat gaatacttcg tcagggggga tgttgcagcg tcggcggcac
    10861 gcacatcgtc gcgtggcggc ggcagatctt gcgctgttca cgcgccaact ggcaacgctg
    10921 gtacaggcag caatgccgct ggaaacctgc ttacaggcgg tcagtgagca aagtgaaaaa
    10981 ctgcatgtaa aaagcctcgg aatggcgctg cgcagccgga ttcaggaagg ttacaccctg
    11041 tcggacagcc tgcgcgaaca tccccgcgtc tttgattctc tgttttgttc gatggttgct
    11101 gccggagaaa aatccgggca tctcgacgtg gtgcttaatc gcctggcaga ttacaccgaa
    11161 cagcgacagc gcctgaaatc acgtctattg caggccatgc tctatccgct ggttctgctg
    11221 gtggtggcaa cgggcgtagt cactattttg ctgacggcag tggtgccgaa aatcatcgaa
    11281 cagtttgatc atctcggaca cgcgctaccc gcctcaaccc gcacgcttat tgccatgagc
    11341 gacgcgttac aggccagcgg cgtttactgg ctggcgggat tgctggcgct tctggtgctg
    11401 gggcaacggc tacttaaaaa tcctactatg cgcctgcgct gggataaaac cgtgctgcgt
    11461 ctgcccgtga cagggcgtgt tgcgcgcggg ctgaatacgg cgcgtttttc ccgcacctta
    11521 agcatcctca ccgccagcag tgttccgcta ctggaaggta ttcagaccgc tgccgctgtg
    11581 tcggcaaatc gctatgtcga acaacaacta ctgctggcgg cagatcgcgt ccgcgaagga
    11641 agcagtctgc gcgccgcgct ggcggagttg cgcctgttcc ccccgatgat gctgtacatg
    11701 atcgcctccg gcgaacagag cggcgaactg gaaaccatgc ttgagcaggc cgctgttaac
    11761 caggaacggg aatttgatac ccaggtgggg ctggcgttgg ggctgtttga gcctgcgctg
    11821 gtggtgatga tggcgggcgt ggtgctgttt attgtcatcg ccatcctcga gccgatgctg
    11881 caactgaata atatggttgg aatgtaattt acggagttat cacatgaatt cgttatcccg
    11941 cacacaaaaa ccacgggcag gttttaccct gctggaagtg atggtggtga ttgttattct
    12001 tggcgtcctg gcaagtctgg tggtgcctaa cctgttgggc aacaaagaga aagccgatcg
    12061 gcaaaaagcc atcagcgata tcgtggcgct ggaaaacgca ctggatatgt accgactgga
    12121 taacgggcgt tatccgacca cagaacaggg gcttgaggcg ctgatccagc agccagccaa
    12181 tatggcggac tcccgcaact accgtactgg tggatacatt aaacgactgc caaaggatcc
    12241 gtggggcaat gattatcagt atctcagtcc gggtgaaaaa gggctgtttg atgtttatac
    12301 cctgggggcg gatggtcagg aaaacgggga aggcgctggc gcagatatcg gtaactggaa
    12361 tttgcaggag tttcagtaag cagtgcctga acgcggattc acacttctgg aaatcatgct
    12421 ggtgattttt cttatcggcc ttgccagtgc gggcgtggta cagacttttg cgaccgattc
    12481 tgaatcgccc gcgaaaaaag cggcgcagga ttttctgact cgctttgcgc agtttaagga
    12541 cagggcagtg atcgaaggga aaacactcgg tgtgctaatc gacccgcctg gctaccagtt
    12601 tatgcagcgt cgtcaggggc agtggtcgcc cgtttcctcg acccggttat cggcacaggt
    12661 tacggtgccg aaacaggtgc agatgctgtt acaacccgga agtgatatct ggcagaagga
    12721 atatgcgctg gagctgcaac gtcgtcgact gacgctgcac gatattgaac tggagctgca
    12781 aaaagaggcg aaaaagaaga ctccgcagat ccgtttttcg ccttttgaac ccgccgcgcc
    12841 gtttacgctg cgcttctact cggcggcgca aaacgcatgt tgggcggtaa aactggcgca
    12901 cgatggcgcg ttatccctca atcaatgtga tgagaggatg ccatgaagcg cggatttacc
    12961 ttgctggaag tgatgctcgc gctggcgatt tttgcgctgg ctgccacggc ggtgttacag
    13021 attgccagcg gtgcgctgag taatcagcaa attcttgagg aaaaaacggt agcgggctgg
    13081 gtggctgaaa accagaccgc gctgctctac ctgatgaccc gcgagcagcg ggcggtcagg
    13141 cagcagggcg agagcgatat ggcaggaagc cgctggtact ggcgaaccac accgctgagt
    13201 acaggcaacg cgctgctcca ggcggtggat attgaagtca gccttcacga agacttttcg
    13261 tcggtgattc agtcacgacg cgcctggttt agcgccgtgg gaggccaaca gtgagaagga
    13321 ctcgcgctgg tttcacgtta ctggaaatgt tggtggcaat tgccattttt gcctcgctgg
    13381 cactgatggc gcagcaggtg acaaacggtg tcacacgcgt gaataacgcc gtcgccggac
    13441 atgatcaaaa actaaacctc atgcagcaaa cgatgagttt tctgacccac gatctgaccc
    13501 aaatgatgcc gcgtccggta agaggcgaac agggccagcg tgaaccagcg ttactggcgg
    13561 gcgctggcgt gctggcgtct gagagtgaag gcatgcgctt tgtgcgcggc ggcgtggtta
    13621 acccgttgat gcgtctgcca cgcagcaatc tgctcaccgt cggttaccgc attcatgacg
    13681 gttatctcga acggttagcc tggccgctga ccgatgccgc aggcagcgtg aagccgacaa
    13741 tgcaaaagtt gattccggcg gattcgctcc gtttgcagtt ctatgatggt acgcgctggc
    13801 aggagagctg gtcgtcagta caggcgatcc ctgtggcagt gcgcatgact ctgcattcgc
    13861 cgcaatgggg cgagattgaa cgcatctggt tgttacgcgg gccgcaatta tcatgatcac
    13921 ctcaccacca aaacgcggaa tggcactggt cgtggtgctg gtattgctgg cggttatgat
    13981 gctggtaacc atcacgcttt ccgggcggat gcagcaacaa cttgggcgaa cgcgcagcca
    14041 gcaggagtac cagcaggcgc tgtggtacag cgccagtgcg gaaagcctgg cgctgagcgc
    14101 gctcagtctg agcctgaaaa atgaaaagcg cgtgcatctg acacagccgt gggcatccgg
    14161 ccctcgtttc ttccctctgc cgcaggggca aattgcggtc accctgcgtg acgcacaggc
    14221 ttgctttaac ctgaatgccc ttgctcagcc gacaacggcg tcgcgtccgc ttgcggtaca
    14281 acagttgatt gccctgatct cgcgcctgga tgtgcctgct tatcgggccg aactgatagc
    14341 cgaaagcctg tgggagttta ttgacgagga ccgcagcgtc cagacacgtc tgggccgtga
    14401 agacagcgag tatctcgccc gttcggtgcc tttctacgcc gcgaatcaac cgctggccga
    14461 tatcagcgag atgcgcgtgg tgcagggaat ggacgacggg ctttatcaaa aactgaaacc
    14521 gctggtctgt gcgctgccga tgacccgcca gcaaatcaac atcaatacct tagacgtcac
    14581 gcaaagtgtg cttcttgagg cgctgtttga cccgtggtta agccctgttc aggcgcgggc
    14641 attattacaa caacgtccgg cgaagggctg ggaagatgtc gatcagtttc ttgcacagcc
    14701 gctacttgct gacgtcgatg agcgtactaa aaaacagcta aaaaccgtcc tgagcgtgga
    14761 cagcaattac ttctggctgc gttcagatat caccgtgaat gagattgaac tgacgatgaa
    14821 ttcgttaatt gtccgcatgg gcccacaaca cttttcggtt ctctggcatc agacaggaga
    14881 aagtgagtga gttccatcct tgagattttt ttcccgcttt gcgcctccga taccgtccgc
    14941 tggcagcgcc gtacacccga cgtggaacac ggtatctggc ctgacgttgc tgacgaacag
    15001 cttcagcaat ggctgcaaac cgatgcgatt cgactctaca ttcccggcga atggatcagc
    15061 gtctggcagg ttgaactgcc tgatgtcgcc cgcaagcaga taccgactat tctgcccgcc
    15121 ttgctggaag aagagctgaa ccaggatatc gatgaactgc atttcgcccc gttgaaaatc
    15181 gaccagcaac tggcaaccgt agcagtgatt caccaacagc atatgcgcaa cattgcgcag
    15241 tggttgcagg aaaacggcat cacccgcgct accgtcgcgc cggactggat gtccattcct
    15301 tgtgggttta tggctggcga tgcgcaacgg gttatctgcc gcatcgatga atgccgggga
    15361 tggagcgccg ggctggcgct ggctccggtc atgttccgcg cccagctcaa tgagcaggat
    15421 ttaccgcttt cgctaaccgt agtcggcatt gcaccggaaa agctatcagc atgggctggc
    15481 gcagacgctg aacgcctgac cgttacagct ctgcccgccg ttaccactta tggcgaaccg
    15541 gaagggaacc tgctgacagg gccgtggcag cctcgcgtca gctaccgaaa acagtgggcg
    15601 cgctggcggg tgatgattct gccgatattg ctgattctgg ttgcgctggc agtggagcgg
    15661 ggcgtgacgt tatggagcgt cagcgaacag gtggcgcaaa gccgcaccca ggcggaggaa
    15721 cagttcttaa cgttgttccc ggagcagaag cggattgtga atttacgctc tcaggtgacg
    15781 atggcgctga aaaaatatcg cccacaggcc gacgataccc ggctgctcgc cgagttgtca
    15841 gcgatagcca gcaccctgaa atcagcgtca ctttccgaca tcgaaatgcg tggtttcacc
    15901 tttgatcaaa aacgccagac gcttcacctc cagttgcggg ctgctaactt tgccagtttc
    15961 gacaaactgc gtagtgcact ggcaaccgat tatgttgtgc aacaggacgc attacagaaa
    16021 gagggtgatg cggtttccgg cggcgtaacg ttgcggagga aataacatgt tacgcgataa
    16081 atttattcac tattttcagc aatggcgtga acgccagtta agccgtggcg aacactggct
    16141 ggcacaacac ctggcggggc gttctccgcg tgaaaagggc atgttactgg cagcggtggt
    16201 gttcctgttt agcgtcggat attacgtcct catctggcag ccgttgagcg aacggataga
    16261 acaacaggag acaatgttgc agcaactggt ggcgatgaac accagactga ggagcgccgc
    16321 gccggatatt atggcggcgc gaaaatccgc cacaacaacg cctgcgcagg tatcgcgggt
    16381 tatcagtgac agcgcttccg cgcactcggt agtcatcaag cggatagccg agcgtgggga
    16441 gaatattcag gtctggatag atccggtggt gtttaatgac ctgctgaact ggttaaacgc
    16501 gctggatgaa aagtatgcgc tgcgggtgac acaaattgat gccagtgctg ctgagaagac
    16561 tgggatggtg aatgtgcagc ggctggagtt tggacggggg taaagtatca ggagattagt
    16621 atcaataaaa ttactctcgt taacgtggcg agagtaattc ccccagactt tataagcaaa
    16681 cttttttcgt gaaggttatt gtcagatcca aaagcaaaaa cccgccttgt gggcgggttc
    16741 ttaagaatta gtggtgcccg gactcggaat cgaaccaagg acacggggat tttcaatccc
    16801 ctgctctacc gactgagcta tccgggcaac ggggcgcatt aaacctgatt cgcctcgtct
    16861 cgtcaatcaa atttcttcaa tttactgcag actgcacaat ctatcatcac tttgccgcta
    16921 ttgcacgcat tgtcaggcaa accaggcggt ccaggcggcg gaaagcggca ttgccagaag
    16981 gagtgctgga agcatgttga ccaccgggaa cattttaatg ccacagatgc gtaatccggt
    17041 tgccagcagc aataaaccgc ctacggcgct gaagtctgcc atcatcgacg gtgtggtcag
    17101 cggtaatatc agcgcggcag cccacgccag cgttaactgg atgatcagta atgggatact
    17161 aattaccgac accgcaatac ccagcgagca ggcgaagatc atcgccgtaa agaaatcaag
    17221 aaatgacttg gcgattaaaa tactcggatc gccggtcatc ccttcgttca tcgccccgaa
    17281 gatcccggtg ccgctggcgc aaaacaggac aataatcgcg acataattct gaataaaaga
    17341 ttcatgcgct ggcttcttac gtgagtgacg aaacagattt tgtgctttgg cgactgctgt
    17401 attgacacct ttctccagca aacaaatttc gccaattaga gcgccaagta gcgtagctaa
    17461 aaccatcgct ggaaggttgg cgcatttcac aaccagtaaa ataccaatcc ccagcgatgc
    17521 cagaccaaaa atagatgtca tggagacgcg gatacgttcc ggtaagcgtt ggctgagcag
    17581 tgcgccgaga acgccaccca gtaagactgc actagcgttg ataaaagggc cgatgaccac
    17641 aagagttcct gttagctgta ttgtttgatt ttcttattat gaactttttg ccttgttgat
    17701 ggcttgtctg gtcgcaaagt cctggcttgc acgctttagc gaaaggtgcc atgattgcgc
    17761 gaattttctc ctctctgtac ggagtttgcc cgatgcacgc catctcctta cattctctcg
    17821 cttatcgccg tttcgcgcga aactcttctc tttttatgct actgctcatg cggtgaagtt
    17881 aacgcacgtt cactgcagga caacagtaaa atcagagcgt ttctgctttt actgatgtct
    17941 ggcggtcgga gctggtgacc agtttgaccc atatctcatg gggcagggtt ttccaccttg
    18001 tccggtattc ttacttcccc gaaacgggtt tgcgcttatg aaatcaatga atattgccgc
    18061 cagtagtgaa ctggtatccc gactttctac tcatcgccgc gtggtggcgt tgggagatac
    18121 tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg gatagtcgca gtggcattct
    18181 tacgttgctt aagcgcaccg gttttcatct accggtgttt ttgtattccg aacatgctgt
    18241 tgaattacct gcgggcgtta cggcggtaat caacggcaac gagcagcatt ggctggagct
    18301 ggaatccgca gcctgtcagt atgaagagaa tttgctgcca ccgttttatg acacgctgac
    18361 gcagtacgtt gagatgggca acagcacctt tgcttgccct gggcatcaac atggtgcgtt
    18421 ttttaaaaag catcctgccg gacgccattt ttacgatttc tttggtgaga acgtctttcg
    18481 cgccgatatg tgtaatgctg acgtaaaatt gggcgatctg cttattcatg aaggatcggc
    18541 gaaagatgcg cagaaattcg cagccaaagt ctttcatgcc gataaaacct attttgtgct
    18601 gaacggcaca tcggcagcga ataaagtggt gacgaatgcg ctgttaacgc gtggcgatct
    18661 ggtgctcttc gaccgtaaca accataagtc gaatcatcac ggcgcgctga ttcaggcggg
    18721 ggcgacgccg gtctatctgg aagcttcacg caacccgttt ggtttcattg gcggtattga
    18781 tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt cgcgacgttg cgccagaaaa
    18841 agccgacctg ccgcgcccgt ttcgcctggc gattattcag ctgggaacct atgacggcac
    18901 tgtctataac gcccgtcagg tgatcgatac cgttgggcat ctgtgtgatt acattctgtt
    18961 tgattccgcg tgggtcggtt atgaacaatt tatcccgatg atggcggata gctcgccgct
    19021 gctgttagaa cttaacgaaa acgatccggg gatttttgtg acccagtcgg tgcacaaaca
    19081 gcaggctgga ttctcacaga cgtcgcagat ccataaaaaa gataaccata tccgcggaca
    19141 ggcgcgtttt tgcccgcata agcggttgaa taacgccttt atgctccatg cttctaccag
    19201 cccgttctac ccgctgtttg ccgcgctgga cgttaacgcc aaaattcatg aaggggagag
    19261 tgggcgtcgg ctgtgggctg agtgcgttga gttgggtatt gagtcgcgca aggctattct
    19321 tgcgcgctgt aagctgttcc gcccgtttat cccccccgtt gttgatggca aattgtggca
    19381 ggattatccg acatcagtgt tagccagcga ccgccgtttt ttcagttttg agccgggggc
    19441 gaagtggcac ggctttgaag gatatgccgc ggatcagtat tttgttgatc cgtgcaagct
    19501 gttactcact acaccaggta tcgatgccga aaccggcgaa tatagcgact ttggcgttcc
    19561 ggcgacgatt ctggcgcact atctgcgtga gaacggcatt gtgccggaga agtgcgatct
    19621 caactccatt ctgttcttat taactccggc ggaaagccac gagaagctgg cacaactggt
    19681 ggcgatgctg gcgcaatttg aacagcatat tgaggatgac tcgccgctgg ctgaggtgtt
    19741 gccgagcgtt tataacaaat atccggtgcg ctatcgcgac tacaccctgc gccagttgtg
    19801 tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa gacctacaaa aagcgatgtt
    19861 ccgccagcag agtttcccgt cagtggtgat gaacccccag gatgcgcata gcgcttatat
    19921 tcgcggtgac gtggagttgg tgcggattcg tgatgccgaa gggcgaattg cggcagaagg
    19981 ggcgttgcct tatccacctg gcgtgctttg cgtggtaccc ggggaagtct ggggtggggc
    20041 ggttcaacgt tatttccttg cactggaaga aggggtgaat ttgttgccgg gattttcgcc
    20101 ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc atgaaacggt tgtacggtta
    20161 tgtgttgaag taagaataaa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
    20221 aattagtggc taaccgtctg tgtacctgtc gggacacgaa cgtgtttata tttgaacatc
    20281 gccatgaacg cgaaggccag aaccacggag taaccagcga aaatcaacca tacggtctgc
    20341 cagtcggtaa tgccgttttg ggtgtacatc tcaaccactt taccgctcac gatgccaccg
    20401 aggatacagc cgaagccgtt ggtcatcatc aggaacatcc cttgcgcact ggcgcgaatc
    20461 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaacgca
    20521 cagccgtaaa caatcatcga cagaaccagc agtacggtgc cgaacggagt cgggtcgccg
    20581 taagcaaaca gcgcaaaacg caggatccac gccacgatac tgatcatcat tacgttctta
    20641 atgccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
    20701 gaaatcgaca tgatgattga cgcatgctgc acaataaagc tgctggcaaa catcggatct
    20761 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
    20821 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
    20881 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
    20941 ggcagagtca gggtaaacag aaccagaatg gcggaaagag ctgcgccaat atacagctgc
    21001 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
    21061 gtgccccaga tacggattgg tgggaagtca gtaacgatat ccatcccggc attttgcagg
    21121 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
    21181 aatatcacaa ggaacatcgc ctccggcgtc gtgacctgtg ctgccatgaa cagcgtgata
    21241 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
    21301 atccccagca gcgcaggcat aaagaccgcg gcgataccca gtgagctata aactgcgcca
    21361 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
    21421 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
    21481 ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
    21541 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
    21601 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
    21661 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
    21721 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
    21781 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
    21841 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
    21901 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
    21961 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
    22021 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
    22081 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
    22141 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
    22201 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
    22261 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
    22321 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
    22381 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcaat
    22441 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg
    22501 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
    22561 ttcacgtagt ccttaggacc agcgatcacc acctctttga atccccagat gttctcaata
    22621 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tgtctttgac ccaggcttcg
    22681 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
    22741 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
    22801 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
    22861 cacctcttta ccctcgaaca ggaagttgac catctcctgc tccagcagct tgcggtgctc
    22921 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgtttgt gctgccactg
    22981 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
    23041 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
    23101 aaatcatcct ctttatcggc tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
    23161 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
    23221 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
    23281 atctaagtgg aaatggctga aggtatggcg aaacgcggtc agctgcgtca ggttatcggc
    23341 agcaatctgc cgttgcgcca gccaatgccg caaactttct tcgtcggcaa actgcgggaa
    23401 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
    23461 gtgctgtaat agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
    23521 cggataaagc gcccagctat tgttggcggc ggcaatacat ccgttttgta gcggacagag
    23581 cgaacatttc ggtttcgagc gcgtacaaat catcgcaccc aaatccatca tcgcctgatt
    23641 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctt aaactccata atttattctc
    23701 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
    23761 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
    23821 ggaacgcccg acgcccggca gtgctgcaac ttcctcaaag gtttccggga atttaccgcc
    23881 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
    23941 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
    24001 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
    24061 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagagt
    24121 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
    24181 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
    24241 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
    24301 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
    24361 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
    24421 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
    24481 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
    24541 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccccga
    24601 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
    24661 gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
    24721 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
    24781 caaagcgcgc cataataaac gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag
    24841 caaactgcag ctggggggcg tattccatat ggcgaccgac tgggaacctt atgcggaaca
    24901 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
    24961 cgtaccgcgt ccggcatcac gtccagtgac gaaatttgaa caacgtggtc atcgtcttgg
    25021 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
    25081 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
    25141 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
    25201 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
    25261 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcaat tgtgcgtaag
    25321 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
    25381 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
    25441 tgcctggaaa gatgcacgat cgagtaggcc ggataaggcg tttacgccgc atccggcatg
    25501 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat ttgttcttga
    25561 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
    25621 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
    25681 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
    25741 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
    25801 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
    25861 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
    25921 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
    25981 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
    26041 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
    26101 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
    26161 ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa aagatttcca gcagtttggc
    26221 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
    26281 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
    26341 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
    26401 acgtcaaatt tcccatacaa agctaaggga taatgcgtag cgttcacgta actggaggaa
    26461 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
    26521 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
    26581 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
    26641 tgcggtgccg caactgaagg acattgcgaa cgttaaaggt gagcaggtag tgaatatcgg
    26701 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
    26761 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
    26821 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
    26881 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
    26941 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
    27001 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
    27061 tcctctgggc tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
    27121 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
    27181 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
    27241 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
    27301 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
    27361 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
    27421 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
    27481 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
    27541 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
    27601 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
    27661 cttatgttga tgactggtaa tgcttgggca gatggcgaac cgccaactga aaatatctta
    27721 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
    27781 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
    27841 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
    27901 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
    27961 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
    28021 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc
    28081 tttagtcagc ttgaatctgc gttgaatacc cagaagaact cgattcctgc gctggaaaag
    28141 gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg
    28201 ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag cctttaaaaa aattcaccaa
    28261 cagcgtgatg agtttaataa acagaacgat agcgaggcgt tcgcggttaa atatgacaaa
    28321 gaggtttatc aaccggcgat tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg
    28381 ccgattcagc agaaacgaga tttcgatatc aacgaacaac ggcgacagac ttttctgcaa
    28441 tcacaaaaac tcagccgtaa attgcaggat gactgggtaa cgctcgaaaa agggcaatat
    28501 ccgttaacga tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt gatgaaaatt
    28561 gacgatatca accaagctaa tgaacgctgg aaaaaagata ccgaacaact gcggcgcaat
    28621 ggggtgatta agtaagtttg atatagtgca cgatttgtag caggatgcgg cgttgacccc
    28681 gcatccggca atcaacgtct gatgcgacgc ttgcgcgtct tatcagacct acaaccgttg
    28741 ccacatgtag gctggataag gcgttcacgc cgcatccggc aatacaagtt tactcagcca
    28801 gaaaaagctc cagcagcgaa tttaaaaaca gcttcccatg ttccgttatc tgccagtaat
    28861 ccgcacattc ggtgagataa ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat
    28921 cttcgcaaag cccggtatac gcaataaact ccacgcgcgg cgcggcctcc agcagacgga
    28981 agcgattcat aaagaactca aacggcttat ctgcggcttc gacatcacgc tggctttcca
    29041 gataccttcc ctgcataaaa ccacgcggat gacgcgtttt ggtggtacgc agaatgcgcc
    29101 catccgggaa ggtcactttg ccgtgcgcgc cacagccaat accgatgtag tcgccaaatc
    29161 gccagtagtt gagattgtgc tggcactgat aaccgggttt ggcgtaagcg gaagtttcat
    29221 attgctgata acccgctgcg gttaataact gatgcccctg ttcgaaaata tcccacagcg
    29281 cgtcatcgtc cggcagcacc ggtggtcgcg aaccaaacag cgtattgggt tcgatggtca
    29341 gttgatacca ggaaagatgc ggcggattca gttcaatggc ctggcgtaga tcgccaagcg
    29401 cctcttccag tgattgatcc ggtagtccat gcatcaaatc aaggttaaag ctacgtaacc
    29461 ctaaaccgct cgccagcttt gccgctcgtt tcgcttcttg cgggccatga atgcgcccga
    29521 gtcgtttcag cttttcttcg ctaaaactct gcacaccaat agagatgcgg ttcacaccag
    29581 cacgctgata atcgacaaag cgatccgctt ctaccgtgcc tgggttcgct tccatagtaa
    29641 tttctgcatc cgctgccagc ggcaaacgcg cacgcacgcc gtccagcagc gtttgcatcg
    29701 ccgggccgga aagcaggctc ggcgtaccac cgccaataaa gattgtcttt acttcacggc
    29761 cctgagcgta agccacatcg ttgtccagat cgttaagcag atgctgaaca taatcgtcgt
    29821 gcggcacttc tcctttcaac gcgtgagagt tgaaatcgca gtacgggcat ttctgcacgc
    29881 accacgggat gtgaatgtag agactcagcg gcggtaattt aaccattacg taaagcgtcc
    29941 agcagcagtt tcaatgcctg accacggtgg gaaatggcgc tcttttcttc gcgggtcagt
    30001 tcggcggcgg ttttcccttc ggaaggtacg aagaagattg gatcataacc aaagccacca
    30061 gtgcccgctg gttcacgagt aatcacgccc ggccagctac cgtggcatac cagcggagtc
    30121 ggatcttccg cgtgacgcag atataccagc acgcagtgga aacgcgcctg acgttggtca
    30181 tccggtacgt ctttcagtgt ttccagcagt ttttgcagat tctttagatc ggtcgcgtct
    30241 tcaccggaat aacgcgcgga gtaaatgccc ggcgcgccgc caagcacatc taccgccaga
    30301 ccagagtcgt cggcaattgc cggtaaaccg gtcacttttg ccgcatggcg cgctttcaga
    30361 atcgcgtttt cgataaaggt caggccggtt tcttcagcgg aatctacgcc gaggtctgtt
    30421 tgggccacga tatcaagacc gaagtcgcta agcagcgacg ccagctcacg cactttaccg
    30481 gcattgccgg ttgcgaggac aactttttgc ataggatacc taattaatta acgccgcgat
    30541 ttctggcggg atttgttgcg gattaatgat tttaatttgt ttgtggcggc caagttcgcc
    30601 tttttcaatc accacctggc ttttggcaac ccggaattgc ttaccgagaa acttcaccag
    30661 atgactgttg gcctgaccgt caaccggcgg cgcggtaatg gcgactttaa cttcgtcgcc
    30721 atgtaaaccg acaatagaat cacggctggc tttcggctga atatagagcc gtaaaaccag
    30781 accgtcatca ttaactgtta cggcactcat aacgccatcc acagccccgg cagcagcatg
    30841 tttccggttg cctgtaatac ttctgcgaca cccatattga tgacatacag cagcagaacg
    30901 aggatcatcg gcgagaaatc aatcccaccc attgccggta gcaggcggcg aatcgggcgc
    30961 agcagcggat cggccagctg aatcagcacg tattcaatcg ggctacgacc ctggcttacc
    31021 cagctcataa tcgccatcac cagcaggacc cagaaaatca gcaggccgat ggttttcagc
    31081 agaatcagta aaccggcaat ccagatgatt ggcaggaagg tcaccacttt aaacagcacg
    31141 atggctttga taaaactgag aatataggca accagcagcg aggcgctgtc aatggggccc
    31201 attgccggaa taacgcggcg cagtggcccg ataattggct gcgttacctt cactacaaac
    31261 tgtgagaagg ggttgtaaaa atcacaatga gcccactgca tccagatgcg taataacagc
    31321 accatggtat acagctcaat gaccgttgaa agcaggaaag tcaacgtatt catggcgttc
    31381 ctttaattcc ttatttttta gagtaatcac gcgcaccaaa aattgcagtg ccgatacgaa
    31441 ccatcgtgct acctgccgca atggcggctt ccatatcgtc cgacattccc agagagagcg
    31501 tgtcgatatg cgggtagcgc gttttcagtc cggcaaatgc tacagccatt tggcgtgcaa
    31561 cttcaaactg ccttacatat tctgactcag gtgcagggat tgccatcagc ccgcgcagac
    31621 gtaaacgcgg tagttcagcg accgcagctg ccagctcgtc cagttctgcc agttgaatcc
    31681 cggacttact gttttcatca ctaatattaa tttgaatcag tacgttaaga gggggaagtt
    31741 ctgccgggcg ctgatcgttg aggcgggtag cgatgcgcaa acggtcgatg gtatgacacc
    31801 agtcgaaatg ctctgccacc aggcggcttt tattagactg caacgggcca ataaaatgcc
    31861 attctaatcc tgttacgccc agttcctgaa agtggcgaat tttatccacc ccttcctgaa
    31921 cgtagttttc accaaattga cgctgcccgg catcaatggc ttctgcgatg gcgctcgcag
    31981 gttttgtttt actgactgca agcagcgtaa tttcttctgg agaacggccg caacgcgttg
    32041 cagccgctga gattttgtcc cggacctgtg ccaggttatg cgcaatatcg ttcattttcc
    32101 gaggatctta gtatgaatat ggaagaaatt gtggccctta gtgtaaagca taacgtctcg
    32161 gatctacacc tgtgcagcgc ctggcccgca cgatggcgca ttcgcggcag aatggaagct
    32221 gcgccgtttg atgcgccgga cgtcgaagag ctactgcggg agtggctgga tgacgatcag
    32281 cgggcaatat tgctggaaaa tggccagctg gattttgccg tgtcgctggc ggaaaaccag
    32341 cggttgcgtg gcagtgcgtt cgcgcaacgg caaggtattt ctctggcatt acggttgtta
    32401 ccttcgcact gtccacagct cgaacagctt ggtgcgccac cggtattgcc ggaattactc
    32461 aagagcgaga atggcctgat tctggtgacg ggggcgacgg ggagcggcaa atctaccacg
    32521 ctggcggcga tggttggcta tcttaatcaa catgccgatg cgcatattct gacgctggaa
    32581 gatcctgtgg aatatctcta tgccagccag cgatgtttga tccagcagcg ggaaattggt
    32641 ttgcactgta tgactttcgc atcggggttg cgggctgcat tgcgggaaga tcctgatgtg
    32701 attttgctcg gagagctgcg tgacagcgag acaatccgtc tggcactgac ggcggcagaa
    32761 accgggcatt tggtgctggc aacattacat acgcgtggtg ccgcgcaggc agttgagcga
    32821 ctggtggatt catttccggc gcaggaaaaa gatcccgtac gtaatcaact ggcaggtagt
    32881 ttacgggccg tgttgtcaca aaagctggaa gtggataaac aggaaggacg cgtggcgctg
    32941 tttgaattat tgattaacac tcccgcggtg gggaatttga ttcgcgaagg gaaaacccac
    33001 cagttaccgc atgttattca aaccgggcag caggtgggga tgataacgtt tcagcagagt
    33061 tatcagcagc gggtggggga agggcgtttg tgacgttgcc atgcccgatg cgcaagttta
    33121 tagggcctat aaaggcaata aatgtctgat gcgacgctaa cgcgtcttat cagaccttgt
    33181 attatccctc cagtgcagag aaaatcggcc agttttctct gcctgcagtc cgcatgccgt
    33241 atcgggcctt gggttctaac ctgttgcgta gatttatgca gcggactgcc tttctcccaa
    33301 agtgataaac cggacagtat catggaccgg ttttcccggt aatccgtatt tgcaaggttg
    33361 gtttcactat ggaacatgaa cttcattata tcggtatcga caccgctaaa gagaaactgg
    33421 atgtcgatgt gttgcgtcct gatggtcgtc atcgcaccaa aaaattcgct aacaccacta
    33481 aagggcacga tgagctggtg agctggctga aaggtcacaa gattgaccat gcgcatatct
    33541 gcatcgaagc gaccggcacc tatatggaac ctgtcgctga gtgcctttac gatgctggct
    33601 acatagtgtc agtcattaat cctgcgctgg gtaaagcttt cgctcagagt gaaggactgc
    33661 gtaacaagac tgataccgtg gatgcgcgca tgctggcaga gttctgtcgt cagaagcgcc
    33721 ctgcagcctg ggaagcgcct cacccgcttg aacgcgcgtt gcgtgccctg gtagtccgcc
    33781 accaggcgct gacagatatg cacacgcagg aactgaatcg cactgaaacg gcgcgggaag
    33841 tccagagacc gagcattgat gctcaccttc tgtggcttga agcagagctg aagcgtcttg
    33901 agaagcagat aaaagacctg acagacgatg atccggatat gaaacaccgc aggaaactgc
    33961 tggaaagcat cccgggtatc ggagagaaaa catctgcggt attgctggct tatatcggtc
    34021 tgaaggaccg cttcgcccat gccagacagt tcgccgcttt tgcgggtctg acaccacggc
    34081 gttatgaatc aggtagcagt gtgagagggg cgagccggat gagtaaggcc ggacatgtgt
    34141 cgcttcgcag ggcgttgtat atgcccgcaa tggtagccac cagtaagact gagtggggac
    34201 gggcgttccg cgaccgtctg gcggctaatg gcaagaaagg aaaggtgatt ctcggcgcga
    34261 tgatgcgcaa gctggcacag gtggcgtatg gagtgctgaa gtcaggcgtg ccgttcgatg
    34321 cgtcacggca taatccggta gcggcgtaaa aatcgcggaa gggatgaaaa aaacagcgcc
    34381 tgacggcgct gtgtctggca tgcctgcaat ccgggaaacc ggaccaggaa aaaactt
//
LOCUS       DAFAVF010000035        33695 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFAVF010000035 DAFAVF010000000
VERSION     DAFAVF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.035"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..658
                     /gene="yccA"
                     /locus_tag="LGK42_001663"
     CDS             <1..658
                     /gene="yccA"
                     /locus_tag="LGK42_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBK2657923.1"
                     /translation="DRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTVL
                     MLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGMG
                     DVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPALH
                     LAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASRD
                     "
     gene            749..1078
                     /gene="tusE"
                     /locus_tag="LGK42_001664"
     CDS             749..1078
                     /gene="tusE"
                     /locus_tag="LGK42_001664"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBK2657924.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(1075..1353)
                     /gene="yccX"
                     /locus_tag="LGK42_001665"
     CDS             complement(1075..1353)
                     /gene="yccX"
                     /locus_tag="LGK42_001665"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK2657925.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            1448..2638
                     /gene="rlmI"
                     /locus_tag="LGK42_001666"
     CDS             1448..2638
                     /gene="rlmI"
                     /locus_tag="LGK42_001666"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBK2657926.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2696..3013
                     /gene="hspQ"
                     /locus_tag="LGK42_001667"
     CDS             2696..3013
                     /gene="hspQ"
                     /locus_tag="LGK42_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBK2657927.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(3058..3471)
                     /locus_tag="LGK42_001668"
     CDS             complement(3058..3471)
                     /locus_tag="LGK42_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK2657928.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            3644..4306
                     /locus_tag="LGK42_001669"
     CDS             3644..4306
                     /locus_tag="LGK42_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBK2657929.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            4402..4860
                     /gene="mgsA"
                     /locus_tag="LGK42_001670"
     CDS             4402..4860
                     /gene="mgsA"
                     /locus_tag="LGK42_001670"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK2657930.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(4892..6946)
                     /gene="helD"
                     /locus_tag="LGK42_001671"
     CDS             complement(4892..6946)
                     /gene="helD"
                     /locus_tag="LGK42_001671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBK2657931.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            7069..7515
                     /locus_tag="LGK42_001672"
     CDS             7069..7515
                     /locus_tag="LGK42_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK2657932.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            7525..9687
                     /gene="yccS"
                     /locus_tag="LGK42_001673"
     CDS             7525..9687
                     /gene="yccS"
                     /locus_tag="LGK42_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBK2657933.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(9650..10279)
                     /gene="sxy"
                     /locus_tag="LGK42_001674"
     CDS             complement(9650..10279)
                     /gene="sxy"
                     /locus_tag="LGK42_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBK2657934.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            10498..11007
                     /gene="sulA"
                     /locus_tag="LGK42_001675"
     CDS             10498..11007
                     /gene="sulA"
                     /locus_tag="LGK42_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBK2657935.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            11364..12416
                     /gene="ompA"
                     /locus_tag="LGK42_001676"
     CDS             11364..12416
                     /gene="ompA"
                     /locus_tag="LGK42_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBK2657936.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(12491..12943)
                     /gene="matP"
                     /locus_tag="LGK42_001677"
     CDS             complement(12491..12943)
                     /gene="matP"
                     /locus_tag="LGK42_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBK2657937.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            13129..14889
                     /locus_tag="LGK42_001678"
     CDS             13129..14889
                     /locus_tag="LGK42_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBK2657938.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14958..15476
                     /gene="fabA"
                     /locus_tag="LGK42_001679"
     CDS             14958..15476
                     /gene="fabA"
                     /locus_tag="LGK42_001679"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBK2657939.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15546..15713)
                     /gene="rmf"
                     /locus_tag="LGK42_001680"
     CDS             complement(15546..15713)
                     /gene="rmf"
                     /locus_tag="LGK42_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBK2657940.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(15969..16532)
                     /gene="pqiC"
                     /locus_tag="LGK42_001681"
     CDS             complement(15969..16532)
                     /gene="pqiC"
                     /locus_tag="LGK42_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBK2657941.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(16529..18169)
                     /gene="pqiB"
                     /locus_tag="LGK42_001682"
     CDS             complement(16529..18169)
                     /gene="pqiB"
                     /locus_tag="LGK42_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBK2657942.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(18174..19427)
                     /gene="pqiA"
                     /locus_tag="LGK42_001683"
     CDS             complement(18174..19427)
                     /gene="pqiA"
                     /locus_tag="LGK42_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBK2657943.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(19557..21464)
                     /locus_tag="LGK42_001684"
     CDS             complement(19557..21464)
                     /locus_tag="LGK42_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2657944.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(21476..23584)
                     /gene="rlmKL"
                     /locus_tag="LGK42_001685"
     CDS             complement(21476..23584)
                     /gene="rlmKL"
                     /locus_tag="LGK42_001685"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBK2657945.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            23828..24937
                     /gene="ycbX"
                     /locus_tag="LGK42_001686"
     CDS             23828..24937
                     /gene="ycbX"
                     /locus_tag="LGK42_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBK2657946.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADDSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(24934..25476)
                     /gene="zapC"
                     /locus_tag="LGK42_001687"
     CDS             complement(24934..25476)
                     /gene="zapC"
                     /locus_tag="LGK42_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBK2657947.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     NPSGFCVDDAALYFSFEEKCRDLPLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMQGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(25650..26660)
                     /gene="pyrD"
                     /locus_tag="LGK42_001688"
     CDS             complement(25650..26660)
                     /gene="pyrD"
                     /locus_tag="LGK42_001688"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBK2657948.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLIENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMD
                     KIYPYAGYIAINISSPNTPGLRTLQYGEALDDLLIAIKNKQNDLQKIHQKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGATLVQIYSGFIFKGPPLIKEIVS
                     NI"
     gene            complement(26771..27508)
                     /locus_tag="LGK42_001689"
     CDS             complement(26771..27508)
                     /locus_tag="LGK42_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2657949.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADKESIAILLTNTSQESWLINSKINRPTRWAGGEVSTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(27474..27989)
                     /locus_tag="LGK42_001690"
     CDS             complement(27474..27989)
                     /locus_tag="LGK42_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2657950.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(27997..28539)
                     /locus_tag="LGK42_001691"
     CDS             complement(27997..28539)
                     /locus_tag="LGK42_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657951.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(28551..29621)
                     /locus_tag="LGK42_001692"
     CDS             complement(28551..29621)
                     /locus_tag="LGK42_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657952.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKSSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(29612..32212)
                     /locus_tag="LGK42_001693"
     CDS             complement(29612..32212)
                     /locus_tag="LGK42_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBK2657953.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(32237..32938)
                     /locus_tag="LGK42_001694"
     CDS             complement(32237..32938)
                     /locus_tag="LGK42_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2657954.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(33021..33563)
                     /locus_tag="LGK42_001695"
     CDS             complement(33021..33563)
                     /locus_tag="LGK42_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2657955.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         8175 a         8803 c         8007 g         8710 t
ORIGIN      
        1 ggatcgtatt gttagttctt cacatgaccg tacatcactg cttagcaccc ataaggtgct
       61 gcgtaatacc tattttctgc tgagcctgac gctggccttt tcggcgatta ccgcaactgc
      121 cagtacggtg ctgatgctgc catctccggg tctgattctg acgctggtgg gtatgtatgg
      181 tttgatgttc ctgacctata aaacggcgaa taagccgacc gggattatct ccgcattcgc
      241 ctttaccggt tttctgggtt atatcctcgg acctattctg aacacctatc tgtctgccgg
      301 aatgggtgac gtaatcgcta tggcactggg cggaacggcg ttagtgttct tctgctgctc
      361 tgcatatgtg ctgaccaccc gcaaagatat gtcgttcctc ggcggtatgc tgatggcggg
      421 tattgtggtg gtgctgattg gtatggttgc taatatcttc ctgcaactgc ctgctctgca
      481 tctggcgatc agcgcggtct tcattctgat ctcctctggc gctatcttgt ttgaaaccag
      541 caacatcatt catggcggtg agacgaacta tattcgtgcc acggttagcc tgtatgtttc
      601 gctgtacaac atcttcgtca gcctgctgag cattctgggc ttcgctagcc gcgattaatc
      661 tcacccgcta acactttcat agcctcgctt tatgcggggc tttgtttttt gttacactgc
      721 cgccagtctg attaaggtag taataattat gctgatcttc gaaggtaaag agatagaaac
      781 ggataccgaa ggctatctca aagaaagcag ccagtggagt gagccgctgg ctgtggtgat
      841 tgcagagaac gaagggattt cgctgtcgcc agaacactgg gaagtggtgc gttttgtgcg
      901 tgatttctat ctggaattca atacttctcc ggcgattcgt atgctggtaa aagcgatggc
      961 gaataaattt ggcgaagaaa aaggtaacag ccgctattta tatcgactgt ttccgaaagg
     1021 tccggctaag caagccacca aaattgctgg cctgccaaaa ccggtaaaat gtatttaacg
     1081 gatacgaaaa tcggctaatt cccccgaggg atgatgcggc tcgctaagca cccgttccac
     1141 acgcgcagaa cgcgggccgc cactctttaa ccactgcatt aatttttcca cctgcccttc
     1201 ttcaccgcag gcaaccactt ccacgctgcc atcgtcaaga tttttggcgt acccggttaa
     1261 acccagtctt ttcgcttcat actgtgtggt gtagcgaaat cctacgccct gaactcgccc
     1321 gtaaacccag gcaattatgc agactttcga cattgctgct ctccttatcc ttaatggacg
     1381 cgttgcaaat ccatggaaaa ctccggacaa tgacgcccat ttctttgcat cgacagaaaa
     1441 gtaaattatg agtgtacgtt tagtgttagc caaagggcgc gaaaaatcat tacttcgtcg
     1501 ccatccgtgg gtcttttccg gggccgttgc ccgcatggaa ggtaaagcca gcctcggtga
     1561 aaccatcgat attgttgatc atcagggaaa atggttagca cgcggcgctt attcaccagc
     1621 ttcgcaaatc cgggcgcgcg tctggacgtt tgacccgtct gagtctatcg acattgcttt
     1681 tttttcccgc cgtttgcaac aagcacaaaa atggcgtgac tggctggcgc aaaaagatgg
     1741 cctcgacagc tatcgtttaa tcgccggaga atctgatggc ctgccgggta ttactatcga
     1801 tcgtttcggt aattttctgg tgctgcaact gctgagtgct ggcgcagaat atcagcgcgc
     1861 ggcattaatt agtgccctgc aaacgctgta cccggaatgt gcgatttacg atcgcagcga
     1921 tgttgcggta cgtaaaaaag aagggatgga gctgacccag ggcctcgtca ccggcgagtt
     1981 gccacctgcc ctgctgccga ttgaagaaca cggcatgaaa ctgctggtgg atattcagca
     2041 cggacacaaa acgggctact acctggacca gcgtgatagc cgcctggcta cccgccgcta
     2101 cgttgaaaat aaacgtgtgc tgaactgttt ctcctatacc ggtggtttcg ccgtatcggc
     2161 actgatgggc ggttgcagcc aggttgtcag cgttgatacc tcccaggaag cgctggatat
     2221 tgcacggcag aacgttgagc tgaacaaact ggatctgagc aaggctgagt ttgtccgtga
     2281 tgatgtcttt aaattgctgc gtacttatcg cgatcgcggt gaaaaatttg acgttatcgt
     2341 gatggacccg ccgaagtttg ttgagaataa aagccagttg atgggcgcgt gtcgtggcta
     2401 taaagatatc aacatgctgg cgattcagtt gctgaatgaa ggcggtattc tcctgacttt
     2461 ctcctgttcc ggtctgatga ccagcgattt atttcagaaa atcatcgcgg atgccgcaat
     2521 tgatgccggc cgtgatgtac aatttataga gcagttccgt caggcagccg atcatccggt
     2581 gatcgctacc tacccggaag ggctatatct gaaagggttt gcctgtcgcg tcatgtaact
     2641 tgaaatgtgg aattttaccc ttatatcaaa ggtaaagatt tcccgggagg tgactatgat
     2701 tgccagcaaa ttcggtatcg gccagcaggt ccgccattcc ctgttaggtt atctcggagt
     2761 ggtcgtggat atcgacccgg tttattcgct ctctgaaccg tcgcctgatg agctggcggt
     2821 gaatgacgag cttcgcgccg ctccgtggta tcacgtggtg atggaggacg ataacggcct
     2881 accggttcat acctacctgg ctgaagccca gctaagtagc gaattgcagg atgaacatcc
     2941 tgaacagcca tccatggatg agctggcgca gaccatccgc aaacaactcc aggccccgcg
     3001 tctgcgtaac taatccctga acgaatcccc ggaaggtttt tgccttccgg ggatttttta
     3061 tttggccagg cccaggcgag ggatctcaat agccgggcag cgatccatca ccacatttaa
     3121 cccggcatcc cgtgccagta ctgccgcttg ctcattaatc acccccagtt gcatccagag
     3181 ggttttcgcg ccaatggcaa tagcttcctg tgccacgccc cacgccgctt ctgaattacg
     3241 gaaaacatcg accatatcga ccttttccgg aacgtctgcc agcgtgccat acccctgctg
     3301 gcccagcagc gttttgccgg caacttttgg cgaaaccgga atgacgtgat acccctggtc
     3361 gagtagatat ttcatcacgc gatagctggg acgatcgggt ttgtcgcttg cccccaccag
     3421 cgcaatggtg tgagtggacg ttaaaatgcc tgcaatatcg gtttctttca ttatttcctc
     3481 cagactgttt aacaaagtgt acgacaaagc cgacatgcca accatgcagc taatacgaat
     3541 gaatgagagc aaaagcggga aatatgtcta tatgttagta agtatgatta aagaaaaatt
     3601 ttgacacatt attattcggg cggtcttttg acaggagcta cctatgaaaa ccggcatcgt
     3661 gaccaccttg attgcattat gtttgccggt atccgttttt gccaccacgc tgcggctttc
     3721 aaccgatgtc gatcttctgg tactcgacgg caagaaggtt tccagctctc tgctgcgcgg
     3781 tgcggacagc attgaactgg ataatggacc gcatcagtta gtgtttcgcg ttgagaagac
     3841 gattcatctt tccaatagtg aagaacggct gtatatctcc ccaccgctgg tggtcagttt
     3901 taatacccag ctcatcaacc aggtcaattt tcgcctgcct cgcctggaga atgagcggga
     3961 agctaaccat tttgatgccg cgccgcgcct tgaattgttg gatggcgatg cgacgccgat
     4021 tccggtaaag ctggatattc tcgccattac ctcaacggca aaaacgattg attatgaggt
     4081 cgaggttgaa cgctataaca aatccgcgaa acgcgcttca ctaccgcaat ttgccacgat
     4141 gatggcagat gacagtacgc tgctctcggg tgtttccgag ctggatgcta ttccaccgca
     4201 gtctcaggtg ctcacagaac aacggctgaa gtattggttt aaactggctg acccacaaac
     4261 gcgaaatact ttcctgcagt gggcggaaaa acaaccatct tcctgagatt tttgtgcctg
     4321 tgcgcaggct ttttcggtct ttatcttgca gcgataagtg cttacagtaa tctgtaggaa
     4381 agttaactac ggatgtacat tatggaactg acgactcgca ctttacctgc gcggaaacat
     4441 attgcgctgg tggcacacga tcactgcaaa caaatgctga tgagctgggt ggaacggcat
     4501 caaccgttac tggaacaaca cgtactgtat gcaacaggca ctaccggtaa cttaatttcc
     4561 cgcgcgaccg gcatgaacgt caacgcgatg ttgagtggcc caatgggggg tgaccagcag
     4621 gttggcgcat tgatctcaga agggaaaatt gatgtattga ttttcttctg ggatccacta
     4681 aatgccgtgc cgcacgatcc tgacgtgaaa gccttgctgc gtctggcgac ggtatggaac
     4741 attccggtcg ccaccaacgt ggcaacggca gacttcataa tccagtcgcc gcatttcaac
     4801 gacgcggtcg atattctgat ccccgattat cagcgttatc tcgcggaccg tctgaagtaa
     4861 tattgcacag gtggcaaacg ccacctgttt cttacggttt tctcgccacc ggcacatcca
     4921 gatttttcag tatttccaca aagggagagg gattctcttt gttaaacagt gcccataccc
     4981 gatggcgtgc ccgggtcagc gccacgtaca ttaaccgccg ttcttcagcg tccgggaaat
     5041 cctcaaccgg tggcagtagc gcctcttcca taatcgactc ccgcgccgca gccggaaaac
     5101 catcacttcc ctcctgcaag ccaacgatga tgacgtaatc cgcctgttgc cctttgctgg
     5161 catgaatggt cataaagtcg atttgcaact tcggccagcg tgttgccgct ttttccaggc
     5221 tggcaggcct catgtgatgg taacgcgcca ggataagaat gcgctcttcc ggtttggcat
     5281 aaccagagag cttatccagc aaagcgtcaa gttgactctc atccaataac gtgacggctt
     5341 tttcgtctcc attggttaag ctgtttagcg gctttttcaa ctggcctggg ttctgctgaa
     5401 taaaccggtt tgccacctca ccgatacgac tgttaaaacg gtaagtcgtg tctaaatcac
     5461 agcgatcgcc ttcaccaaag ttttcatgga aagcggtggt gagcgacatt tgcgcaccgc
     5521 tgaatcggta aatcgcctgc cagtcatcac caacggcgaa caacgtcgtc tgactgtttt
     5581 gcttgcgtaa tgccgctaac aacgctgccc gctgcggcga gatatcctga aattcatcaa
     5641 ccagaatatg cttccacggg ctgataaagc gacctttctc cagaatcaca atcgcctgat
     5701 gaataaggcc cgaaaaatcg acagcgtttt ctgccttcag cgcacctttc caggctttta
     5761 ataacggggc catcaacttg atacgtttac tgaacagatc gcgaatctct tcgggtgcac
     5821 tggcaatcat ttctgcctgt gcaccaccgt gcatccgcat cagacttacc caacgatcga
     5881 ggcgtgaggc aagacgacgc tgtaattttt catcatccca gaagttacct tctggcactg
     5941 accactgcat ttcttccgtc agccattgcc gccagccctt cgcctgcgct ttcttttcgc
     6001 tgcattgctt gcgccactca gcaataaaga gttcatgacg ggcagcggta tcattttcca
     6061 gtttgctgac tatcggaact tttttgctgc cctgctgaat aatatgcagc gccagcgcat
     6121 gaaacgtgcg tgcggtaatg tcttcggtat gtagccgttc gcgaatacgc tcgtccatct
     6181 cttcagcggc tttgcgacca aacgccagca ataaaatttg ctcaggggac gcttcaccac
     6241 gcgccagcaa ccagcctgca cgggccacca gcaccgacgt ttttccgctt cctgcacctg
     6301 ccagcactaa cagagaatgc tcgccattaa cgactgcccg ggcctgcgcc ggattcagcg
     6361 gtgaagactc gacctggcgg aaaaaatccg catactcggt aagcatggct tcggtatacg
     6421 cctggttatg ctgcaaccga gcgctttcaa tatctttcaa ccaggcctga catttacgcc
     6481 acgcctcacg gcagttatcg aattcttcca gtcggttaac cggcaacggc aacgccgaca
     6541 tagcctggcg gatttgttgc tgaacgccag aggtttgctc acgcgtcagc catttatttt
     6601 ctccagtgcg cgtggcaatc aaatccagtt gctggcgtaa aacaccagac gcaatttcgc
     6661 tcatctcgcc actccaccgc cgccagtgag catcaagatg atggtaaaaa cgctgcgtct
     6721 cgccccattc ggtgccgtgc agacgcacca ctttttcgtc cggcagtaca aattccagct
     6781 cgccccatac cagaccgcgc ttacagtgaa tcgccagtaa ttgattgaaa ggaataaggt
     6841 attcgtggcg atcgccggag actttaatcc cggcattgag gatcaccgcc cgatcgtaag
     6901 ggtgctgtgc cagacgtttt ccaagcgttg tcgctttcag ttccatgact ctgtagatcc
     6961 acacgaaatt atttcttatc agtgtaaccg tcagagaaaa cgtgctccag cccaaaaaaa
     7021 cgttacaatt gccgcccatt actgaaaacc acagtaaagc gaggttttat gcgtaccgtt
     7081 ttgaacattc tgaactttgt gcttggcgga tttgccacca ctctgggctg gctgttggcg
     7141 actctggtca gtattgtgct gatttttacc ttaccgctga cacgatcctg ctgggagatc
     7201 actaaactgt ctctggtgcc ttatggcaat gaagctattc atgtcgatga actgaacccg
     7261 gctggcaaaa atgtgctgct gaatactggc ggtacggtat tgaatatttt ctggctgatt
     7321 ttctttggct ggtggttatg cctgatgcac attgcaacgg gcatcgcaca atgtatttca
     7381 atcattggca ttcctgtcgg cattgcgaac tttaaaattg ccgctattgc actatggccg
     7441 gttggtcgtc gcgtggtatc ggtagaaaca gcgcaagctg cgcgtgaagc caatgcacgt
     7501 cgtcgttttg aataatccac taatatggcc tttatgctaa gtcctttgct caaacgctat
     7561 acctggaaca gcgcctggct gtattacgcg cgtattttta ttgcgctttg tggaaccaca
     7621 gcgtttccgt ggtggctggg tgatgtaaaa ctgacgattc cgctaacgct ggggatggtg
     7681 gcagcggcgc tgaccgatct cgatgaccga ctggcgggac gtttgcgtaa cctcatcatt
     7741 acgctgttct gcttttttat cgcctcggcc tcagtagaat tgctgtttcc ctggccctgg
     7801 ctatttgcga ttggcttaac gctctctacc agcggcttca ttttgctcgg cggtctgggt
     7861 caacgctatg caacaattgc cttcggtgca ttgctgatcg ccatttacac tatgttggga
     7921 acatcactgt atgagcactg gtatcagcag ccgatgtatc tgctggccgg tgccgtctgg
     7981 tacaacgtcc tgacacttat tggtcatctg ctgttcccgg tccgcccgct gcaggacaac
     8041 ctggcgcgtt gctatgaaca actggcgcgt tatcttgagc tcaagtcgcg catgtttgat
     8101 cctgatattg aagatgaaag ccaggcaccg ctgtacgatt tggctctcgc caacggacaa
     8161 ctgatggcga cattgaatca gacgaaactc tcgctgctga cccgcttacg tggcgatcgt
     8221 ggtcaacggg gaacgcgtcg cacgctgcat tattactttg tcgcgcagga tattcacgag
     8281 cgtgccagct cttctcatat tcagtatcaa acattgcgtg aacattttcg ccacagcgac
     8341 gtgctgttcc gttttcagcg gctgatgtcg atgcagggcc aggcgtgcca gcaactgtca
     8401 cgctgtattt tgctgcgtca gccttatcaa catgatccgc attttgagcg cgcttttacg
     8461 catattgatg ctgcgctgga gcggatgcgc gataacggcg cacccgccga tttactcaaa
     8521 acactgggat ttttgctgaa caatttacgc gccattgatg cccaactggc aacaattgaa
     8581 tcagaacagg cccaggcact accccataat aatgacgaaa atgagctcgc tgatgacagc
     8641 ccgcacgggt tgagtgatat ctggctgcgt cttagccgtc acttcacgcc ggaatccgcc
     8701 ctcttccgtc atgcggtaag aatgtcgctg gtgttgtgct tcggctacgc catcattcaa
     8761 ataaccggaa tgcatcacgg gtattggatc ttgctgacaa gtttgtttgt ctgccagcca
     8821 aactataacg ccacgcgcca ccgcctgaag ttaaggatta ttggtacgct ggtaggtatc
     8881 gccattggca ttcctgtgct gtggtttgtg ccatcactgg aagggcagct ggtgctgctg
     8941 gttattaccg gcgtgctctt ttttgccttc cgtaacgtgc aatacgctca tgcaacgatg
     9001 ttcatcacac ttttggtgct actgtgtttt aacttactgg gtgaaggttt tgaagtagcg
     9061 ttacctcgcg taatcgatac gctgattggt tgtgccattg cgtgggcggc agtgagctac
     9121 atctggcctg actggcagtt tcgcaatctg ccgcgcatgc tcgaacgcgc cacagaggcc
     9181 aactgtcggt atctcgatgc catactggag caataccatc aggggcgtga taaccgtctg
     9241 gcgtatcgta ttgcccgccg cgatgcacac aaccgtgatg ctgagctggc gtcggtggta
     9301 tcaaatatgt ccagcgagcc gaacgttacc ccgcaaattc gcgaagccgc gtttcggttg
     9361 ctgtgcctta accatacgtt taccagctat atctcagccc tcggtgctca ccgggagcag
     9421 ttaactaatc ctgaaattct ggcgtttctt gatgacgcag tttgctatgt tgatgacgcg
     9481 ttacatcatc aacctgctga tgaagaacgc gtcaatgagg cattagctag cctgaaacag
     9541 cggatgcagc aacttgaacc acgggcagac agcaaagaac ctctggtcgt acaacaagtt
     9601 ggattattga ttgcattact gcctgagatt ggtcgtctgc aacgccagat tactcaagtt
     9661 ccgcaggaaa ctcctgtttc ggcgtaagag agtcagccca ttctgcaagc tcctggcggc
     9721 gtgccaccgg gagcgcagct tcatgaatgc cgataatggc accttcaagc ataaacagaa
     9781 tcttttctgt caccagactg ttttgctgcc gcagtcgcaa ccaacacatt tttgccccaa
     9841 gtatacgtaa cgcccgtaca tccttaatcc ccacctcccc gagaatcgct tccagatgaa
     9901 aagacatatt gggcaaatct ttcagtcttt cccgggtatt gcgcgtgctt ttctctttca
     9961 gcgctgcatc gagagaatat ttcgacagac gcaccagctt cagttgattt cgccatagac
    10021 tttcatcaac ccgatagtaa ttgagggtaa cggatcggcc acacttttta tatgtcagcc
    10081 agacaggcgg atgttttaca cagtactgtg cactttgctc acaagcccga agatacaact
    10141 caccatcaga aaccatcgca aacaccgtgt cgtcaacggt caggctgtaa ctgccaaaca
    10201 atgatcggta ttcaattgtg cccaacgttg ccaggtattc ttgtgattta tagatccgct
    10261 tataggagag gcttttcata aaattccttt taaaatcata acataaaaga atgattcaca
    10321 ttaacggatc cgttaactac gaaaataggc aacttattct taaggggcaa gattaattta
    10381 tgttttcccg tcaccaacga caaaatttgc gaggctcttt ccgaaaatag ggttgatctt
    10441 tgttgtcact ggatgtactg tatatccata cagtaactca caggggctgg attgattatg
    10501 tacacttcag gctatgcaca tcgttcttcg tcgttctcat ccgcagcaag taaaattgcg
    10561 cgtgtctcta cggaaaacac tacagccggg cttatcagtg aagttgtcta tcgcgaagat
    10621 cagcccatga tgacgcaact tctactgttg ccattgttac agcaactcgg tcagcaatcg
    10681 cgctggcaac tctggttaac accgcaacaa aaactgagtc gggaatgggt tcaggcatct
    10741 gggctaccct taacgaaagt aatgcagatt agccagctct ccccttgcca cactgtggag
    10801 tcaatggttc gcgctttacg cacgggcaat tacagtgtgg tgatcggttg gttggcagat
    10861 gatttgactg aagaagagca tgctgaactt gttgatgcgg caaatgaagg taacgctatg
    10921 gggtttatta tgcgtccggt aagcgcatcc tctcacgcca cgagacaact ttccgggcta
    10981 aaaattcact ctaatttgta tcattaagta aatttaggat taatcctgga actttttttg
    11041 tcgcccagcc aatgctttca gtcgtgacta attttccttg cggaggcttg tctgaagcgg
    11101 tttccgcgat tttcttctgt aaattgtcgc tgacaaaaaa gattaaacat accttataca
    11161 agactttttt ttcatatgcc tgacggagtt cacacttgta agttttcaac tacgttgtag
    11221 actttacatc gccaggggtg ctcggcataa gccgaagata tcggtagagt taatattgag
    11281 cagatccccc ggtgaaggat ttaaccgtgt tatctcgttg gagatattca tggcgtattt
    11341 tggatgataa cgaggcgcaa aaaatgaaaa agacagctat cgcgattgca gtggcactgg
    11401 ctggtttcgc taccgtagcg caggccgctc cgaaagataa cacctggtac actggtgcta
    11461 aactgggctg gtcccagtac catgacactg gttttattcc taacaatggt ccgacccacg
    11521 aaaaccaact gggtgcaggt gcttttggtg gttaccaggt taacccgtat gttggttttg
    11581 aaatgggtta cgactggtta ggtcgtatgc cgtacaaagg cgacaacatc aacggtgcat
    11641 acaaagctca gggcgttcaa ctgactgcta aactgggtta cccaatcact gacgatctgg
    11701 acgtatacac tcgtctgggt ggtatggtat ggcgtgcaga caccaaggct aacgtacctg
    11761 gtggcgcatc ctttaaagac cacgacactg gcgtttctcc ggttttcgca ggtggtgttg
    11821 agtacgcgat cactcctgaa atcgctaccc gtctggaata ccagtggacc aacaacatcg
    11881 gtgacgcaaa caccatcggt actcgtccgg acaacggtct gctgagcctg ggtgtttcct
    11941 accgtttcgg tcagggcgaa gcagctccgg tagttgctcc ggctccagct ccggcaccgg
    12001 aagtacagac caagcacttc actctgaagt ctgacgttct gttcaacttc aacaaagcaa
    12061 ccctgaaacc ggaaggtcag gctgctctgg atcagctgta cagccagctg agcaacctgg
    12121 atccgaaaga cggttccgta gttgttctgg gttacactga ccgcatcggt tctgacgctt
    12181 acaaccaggg tctgtccgag cgccgtgctc agtctgttgt tgattacctg atctccaaag
    12241 gtatcccggc agacaagatc tccgcacgtg gtatgggcga atccaacccg gttactggca
    12301 acacctgtga caacgtgaaa cagcgtgctg cactgatcga ctgcctggct ccggatcgtc
    12361 gcgtagagat cgaagttaaa ggcatcaaag acgttgtaac tcagccgcag gcttaagttc
    12421 tcgtctggta gaaaaacccc gcagctgcgg ggtttttttc gcctttagta aattgaactg
    12481 acttcgtcag ttattcctta cccagcaatg cctgcagatc atgcttcaaa gaagacattt
    12541 tattcgcgta tttctctttg ttttccgcat cttcaatcag ctgaacaatc gtttcagaaa
    12601 gcgttttacc gcgacgctgc gcaagaccag ccagacgttg ccagacaata aattccagat
    12661 cgatcgattt tttgcgcgta tgctggtgtt ccgcattaaa atggcgcttc cgtctggccc
    12721 gaatggtttg cttcatgcga ttgaccagtt ccggattcat atgcttgtca atccagccat
    12781 ttaccagcac gggttcattt tccagcgaga gcaacacatc gacggcttcc tgggcggcac
    12841 tggcttctat gtaacgggtg attaactccc cttcgcgatg cttcttcacc agatacttcc
    12901 atttccaacc gctttcaaga ttttcaagtt gttgatattt catagcgatc tcaatgttac
    12961 cgtgtaactc tttacagaat atcagctttt taggccagtg aagaaaagaa tctccatcct
    13021 gtgagcttta acgcccatgc caggagtatt gtcggtgctt tctacgtgtg ctgtagtgcc
    13081 ggttacggta taatcgcggc tttgacaaca gactaaaaaa catcaacttt gaccattacg
    13141 aaacttgcat ggcgtgacct ggttcctgat accgatagct atcaggaaat atttgctcag
    13201 ccacatttga ttgacgaaaa cgatccttta ttcagtgata ctcaaccgcg actgcaattc
    13261 gcactggagc agttgctgca tacgcgagca tcctcctctt ttatgctggc gaaggccccg
    13321 gaagagtctg agtatctgaa tcttattgcc aatgccgcgc gtacgctaca aagcgatgca
    13381 ggccaactgg tgggcggtca ctatgaggtt tccggccact ccatccgctt acgtcacgca
    13441 gtgagtgcag atgataattt tgcgacttta acgcaagttg tcgctgccga ctgggtagaa
    13501 gcggagcaac tctttggctg cctgcgccag tttaatggcg acattaccct gcagcctggt
    13561 ctggtgcatc aggcaaatgg cggtattctc atcatctctt tgcgtacact gctggcgcaa
    13621 cctctgctgt ggatgcggct gaaaaatatc gttaaccgcg agcgttttga ctgggttgcg
    13681 tttgatgagt cgcgccctct ccccgtctct gtgccttcga tgccattgaa gctgaaagtc
    13741 attctggtag gcgaacgcga atcattggct gatttccagg agatggagcc agagctttca
    13801 gagcaggcta tttatagcga atttgaagat actctgcaga ttgtcgatgc agagtcagta
    13861 acccagtggt gtcgctgggt gacatttacc gccagacata atcacttacc tgcaccggga
    13921 gcggatgcct ggccggtact tatccgcgaa gcagcacgct acaccggtga acaagaaaca
    13981 cttccgctta gcccgcagtg gatcctccgc cagtgtaaag aggtcgcctc cctgtgtgat
    14041 ggcgacacct tctccggcga gcagctaaac ttaatgctgc agcagcgtga atggcgcgaa
    14101 ggtttcctcg ctgaacgtat gcaggatgag atccttcagg agcaaatcct gattgaaacc
    14161 gaaggcgaac gcatcgggca aattaacgcc ctttcggtca ttgaatttcc gggtcatcca
    14221 cgcgcttttg gcgaaccttc tcgcattagc tgcgttgtgc atattggcga tggtgaattc
    14281 accgacatcg aacgcaaagc ggagcttggc ggcaatatcc atgcgaaagg gatgatgatc
    14341 atgcaagcgt tcctgatgtc ggaactacag cttgagcaac agatcccctt ctcagcatcg
    14401 ctgacatttg agcagtcata cagtgaagtt gatggagata gtgcctcgat ggctgaactc
    14461 tgcgccctga taagcgccct cgccgatgtg ccggtgaatc agagtatcgc tatcacaggt
    14521 tcagtcgatc agttcggtcg cgcccagccg gtcggtggtt taaatgagaa aatcgaaggc
    14581 ttctttgcta tttgccagca acgtgagtta accgggaaac aaggtgtcat tatccccaca
    14641 gctaacgttc gccatttaag tcttcacagt gaactggtga aagcggtaga agaaggcaaa
    14701 ttcaccatct gggcagtaga cgatgtgact gacgcactgc cgttattatt aaatctggtg
    14761 tgggatggcg aaggccaaac gacgctgatg caaaccatcc aggaacgtat cgcgcaagca
    14821 tcgcaacagg aaggacgtca ccgttttcca tggccattac gttggctgaa ctggtttatt
    14881 ccgaactgat cggacttgtt cagcgtacac gtgttagcta tcctgcgtgc ttcaataaaa
    14941 taaggcttac agagaacatg gtagataaac gcgaatccta tacaaaagaa gaccttcttg
    15001 cctctggtcg cggtgaactg tttggcgcta aaggcccgca attgccagca ccgaacatgc
    15061 tgatgatgga ccgtgtggtc aaaatgaccg aaacgggtgg taacttcgac aaagggtatg
    15121 ttgaagcaga actggatatc aatccggatc tgtggttctt cggatgccac tttattggcg
    15181 atccggttat gccgggatgc ctgggcctgg acgcaatgtg gcagctggta gggttctacc
    15241 tcggctggct gggcggcgaa ggtaaaggcc gcgcgctggg cgttggcgaa gtgaaattca
    15301 ctggtcaggt actgccgaca gcgaaaaaag tgacctaccg tattcacttt aaacgcattg
    15361 ttaaccgtcg tctgattatg ggcctggcgg atggcgaagt gctggttgat ggtcgtctta
    15421 tctataccgc cagcgacctg aaagtcggtc tgttccagga tacgtctgcc ttctgattat
    15481 aatcagacag agtatcaaaa ggcgaaacct ccgcaatgcg gaggtttctt tttaaagaga
    15541 cagaatcagg ccattactac cctgtccgcc atggcttctc gccagcctcc cagccattgt
    15601 gacctttgat tcagcgtctg atagggacac atttcttttg agcgtccggc gatgccggcc
    15661 tgataaccac gttgatgtgc ccgttccagg cgatctcgtt tttgtctctt catgcctcgt
    15721 ttccctcata ctggtttctg gtggaaaaga aaacagtgat tactaaatgt gcaatcacag
    15781 ttaagcaata ccgcgaatca taactgccgt caatgcgcaa aattcacgcc actgtcatat
    15841 ttgtgagcta cacgggcatg tcggttgtac aaaaaatgtg aagaggaaca aatttttccg
    15901 cacgttagaa acaaaaaaac cgcctcaggc aacataccgg agacggttat atttacaaat
    15961 ctttataatt aaggtagacg ctttatctct tgtgcaatag aagcggcttc ctgactccag
    16021 acaccggcca gcactttaac catctcatcg taaccatcct gagtttgcac tccttccaga
    16081 cggaacggac gtttgatcag ttgtccctgg tggttcaaca gccactcacc actgacaatg
    16141 accttgccat catagcgacc gttaaactcc gttacggtaa cattgagcgt gtcctgggcg
    16201 cttcccagag gctgggaggc aaccacccag ccgggcagtt gcgtactcag gttggcaacc
    16261 agggtgttgc gcaactgttg atccaacggg ctggcccaca agttgttgtt ggcaatcaca
    16321 tacttcacat cactggtttg ataaaccaca ccattccccg ccagatagtc aggaacagtg
    16381 acctgctcta cccataacag acgattgccc tggctggcgg tactttgtgt accgctctgc
    16441 accacaggta actggtaata gtttttatta atttcgccgg agctgcatcc gaccagccac
    16501 agtgctgcaa tcgtcactag ccactttttc attgtttcgc cctcttcggc tctggatctt
    16561 ttttgtcctt cgcttcaaat accagcgcgt tactcttctc attgagcgtt ttcagcaccg
    16621 gttgcagttc tcgcaacacc tgatcaaggc gctgcatatc cgccaccatc ttgttgtagg
    16681 ctgcggagcc aggctggaag ccctgcatgc tgcgattcaa ttcacgcaac gttgactgca
    16741 tatccgtcgg caactgctgc atcgactggc tagcgaggat cttgttcatg ctatccagcg
    16801 tcgtttgcag gtttttcatt gtgcgctgac tttcagaaag cgtactggtt gcctgttcaa
    16861 tcatcggatt caatggcagt ttgttgatct tatccaacgc ttccatcagt cgttgctgga
    16921 tttgcgccag gccgccgcta acggtcggga taatctgata accattaaat tcacgaatac
    16981 cggttattgc aggcgtattt ggatagaaat cgagatcaac atacagtgca ccagtgacca
    17041 ggtttccggt tttcagcgat ccgcgtaaac cacgtttcaa caattcgcca aggtgctcaa
    17101 caacatccgc attttcgcca agctgcattt tcagccgctc tggctcgata cgaatcagta
    17161 ccggaatacg gtaatcatcg ttaaatgtct gacgcatatt cggcgcaaag aatggcactt
    17221 tgcttacggt acccaggcga ataccccgga actctaccgg agctcccggt tgcagaccgc
    17281 gtaccgaatc tttaaagaac atcagataat caatgtgatc ggtgtacaac gaatcctgaa
    17341 tgctcttctg atcatcatac aaaacgaaag ctgttttagg tgccactggc tgccctaaat
    17401 ccagaccttc cggcacatca aagctgacac cgccactcag cagcgttgtc aatgagccca
    17461 tctccacacg catccctgct gacgtcagat caaccgcaat gccactatct ttccagaagc
    17521 gaacattgtt ggtcaccagt cggtcataag gtgcattgat gaacagttga tagctgatat
    17581 tgcgtttttg tgtatcgaag gtgctggttt caaccgaacc tacccgatag ccacggaaca
    17641 gcaccggatc tcctggcgag agctgcccgg cttttttgct atcgagaatc acacggatac
    17701 ctttcgcatc aggcggggcc aacggtggcg agtccagcaa atcgtattta tccattttgc
    17761 tgcctttcgc gcctggctgc agttcgatat aaactccaga cagcagcgtt cccaggccgc
    17821 taatcccttc gcgaccaatc tgcggtttca ccacccaaaa gacggtgtct ttatgcagca
    17881 atttttccat accggaattc agccgcgctt tgatttcaac gtgcgtcaaa tcatcagcca
    17941 gtgtggcgct ttcaaccacg ccgacgtcaa cgctacggct tttaatggtg gttttgccac
    18001 cttcaattcc ttccgcattc gcggtgatca gggtcacttc cggtccctga tggctgtaat
    18061 gataaaaaag aacccaggcc ccaatgagcg ccgtgacgat aggaaatatc cacacgggag
    18121 accagttctt cactttctgg attttggctt ccccattatt agattccata ctgtcaggac
    18181 tcctcatgct ctgattcagg ttgacgatcc cacgacaaac gcgggtcaaa cgtcatagca
    18241 gaaaacattg tcattatgac gactaaagca aacattaatg cacccattgc cggataaata
    18301 ctcattaaac ctcccatacg caccagcgcc gagagcaccg cgataacgaa aacgtcaatc
    18361 atcgaccagc ggcctacaaa ctcaacaact tcataaatca aatgcattct ttcactgtcg
    18421 cgcttgccat gccctttggc atcccaacac agccacgcga tggcgatcat ctttaacgtt
    18481 ggcaccataa tactggccag aaagatcacc gcagcgacgg gataagagcc ttcgctccat
    18541 aacagaatga ccccagcgag aatcgtcgac ggcatcttcg agcctaataa atccgtcacc
    18601 atgatgggca aaatattagc cggaagatac agcatgatgg acgttacaag cagcgcgagt
    18661 gtccactgca ggctgtttct acgccgaacg taccctttgg tactacaacg cgggcacacg
    18721 ggttcatcag caggaaggat tgccgtacaa caggagcacg aacgcagccc ctgacgtatc
    18781 cccgtgacgc ctggttttag cggctggcgc agttctggca tcggggcgat gtcgtcccat
    18841 aaccagcgac gatcaacgca ctgaaaagcg cgcagttgca ggacacaaaa taaacaccag
    18901 gggagaaagc tgctgcctac cccaatgctg ccgtaagcca tcagtttaac gaaactgacc
    18961 agcacaccag cgaggaaaat ctccgccatt ccccaggttt tgagttgaaa aagcacccgt
    19021 gccagttgct cttttaaacg gaccggtaat tccgcgcgat tcaccagtaa cagaatggtt
    19081 atcagacaaa acgcgggaac cagttgcaca aacaacagga aaaaggtgcc gaggctggcg
    19141 tagtcctcag aaaaaagcac gccgggaatt tccagtaatg taatttcact ggtaactcct
    19201 gcaacgttca tattcacaaa aggaaacaag ttggacagca acagcatgaa cagtgcagcc
    19261 aacgcatagg cggtaggacg ctgccgaggg gcatcccacg ccacggttaa cgttgtgcca
    19321 caccggggac atgccgcttt ctgaccatgc tcaaggcgcg gtaacgccac cagcatgtca
    19381 cactgcgagc acaggatgtg cttcgcggca tgatgatgtt cgcacattgg tactccttaa
    19441 tgatgcgcta ttttacagat gactaacaaa gtgttggttt tcatgcctga taaacccagt
    19501 tcatcaggca attttaccgt tgtcagctgt ctctgtttaa atcgactatt ttgcgatcag
    19561 ccaccatttt ttaacgcttc aagatactcc cagcgttcaa aggcttgctc cagctcctgc
    19621 tctgcagcag ccatatcggc gagcactttt tgtgtctgct catgcggctg actgaagaag
    19681 gaagcatccg ccacttgcgt ctgtaaagct tccagcttcg cctccagatc ttcaagcaat
    19741 tgcggtagct gctccagttc gcgctgcaat ttatagctta gtttgctact gctgcgtttt
    19801 acagtttctg cttttggcac ggcggcttct tcgctttttt tcaccgcagg ctgtttgagc
    19861 gccacatact gttcttgctg accacgggca tcatgataac cgccaacata acgaccaatt
    19921 ttaccgccgc cttcgaagat ccagcattct gtaacggtgt tatcgacaaa ctgacgatcg
    19981 tggctaacca gcaataccgt gccctgatag ctgtcgatca gttcttccag cagttccagc
    20041 gtttcgacat caagatcgtt ggtcggttcg tcaagaatca ataagttgct tggtttgagg
    20101 aacaaacgcg ccagtagcaa gcggttccgc tcaccgccag aaagcgcacg taccggcgtc
    20161 atcgcccgtt tcggatggaa cagaaagtcc tgcaaatagc ccaatacgtg gcgtggcttg
    20221 ccattaacca tcacctcttg cttaccttcg gcaaggttat ccatcaccgt tttatcggga
    20281 tccagttccg cgcggtgctg atcgaaataa gccacttcca gtttggtgcc aacgtgaata
    20341 cgcccgctgt ccgcttgaag ctgaccgagc atcagtttca gcagcgtggt tttgccgcac
    20401 ccattcggac caatcagggc aattttgtcg ccacgtagaa cctgggccga aaaatctttc
    20461 accagttgct taccgtcaac ctggtagcaa acgtcttcca tttcgaaaac gatcttaccg
    20521 gagcggctgg cctcttccac ctgcatcttt gcggtaccca tcacttcgcg acgttcacca
    20581 cgctcgcgac gcattgcttt cagggcgcgt acgcggcctt cattacgggt acggcgtgct
    20641 ttgatcccct ggcggatcca cacctcttcc tgcgccagtt tgcgatcgaa ctcggcattt
    20701 tgtagttctt ccacgcgcag ggcttcttct ttttcctgca ggtactggtc gtaattcccc
    20761 ggataggtca ccagcttgcc gcgatcgaga tcaacaatgc gcgtcgccat attgcggata
    20821 aacgaacggt cgtgggagat gaaaataatc gtcccattga aggttttaag gaacccttcc
    20881 agccagtcga tggtttcgat atccaggtgg ttagtcggtt cgtcgagcag caaaacgcgc
    20941 ggattgctca ccagcgcgcg tcctaatgcc gctttacgca accagccgcc ggaaagcgac
    21001 gacagcgcaa cattaggatc taaccccagt tgcgccagca cttcgttgat gcggttttcc
    21061 agctgccaca ggttgtggtg atccagctgt tcctgaacct tcgccagttc gttgagattt
    21121 ttctcgctcg ggtcgttcat caccaggcgc gaaatatcgt gatagcgttt caggtattcc
    21181 gcttgttctt caatgccttc ggcaacgaaa tcataaacgc tgccctcaac gttacgcggc
    21241 ggatcctgtt gcagacgagc cacgatcaaa tcttgctcgt aaataatgcg cccgtcatcc
    21301 agcccctgtt cacggttgag gatcttcatt aacgtcgatt tgcccgcgcc gttacggcct
    21361 accagacaaa cacgttcgtt atcttcgata tgcagttctg cgttatcgag aagcggcgcg
    21421 tcgctgaacg acagccatgc accatgcata ctgattaatg acattactat tcctttcagg
    21481 ctgcggtaat cagccagcag ttgtggatct ggcggttacg ggcgaaatcc tgggagagcg
    21541 ttttttgcgt aatttcttgt gctttcagtc ccagttttgc cagcccgtcg agatccatac
    21601 ggaagccacg tttgttgttc gagaacatga tcgtcccacc tgcacgcagc agacgtttca
    21661 aatctttcat cagcgccaga tgatcgcgct gaacatcaaa cgcatcttcc attcgttttg
    21721 agttagagaa ggttggcgga tcgataaaga tcagatcgaa ctgttcattt gcctcacgca
    21781 gccacgccag gcaatcggcc tgaatcaggc gatgcgcacg cccggttagg ccattcagac
    21841 gcaggttgcg ttctgcccac tccagataag tacgcgacat atccacggtg gtggtgctgc
    21901 gtgcaccgcc taatcccgca tgcacggtgg cgctgccggt ataagagaac aggttgagga
    21961 aatctttgcc tttgctcatc tggccgagca tacgacgggc gatacggtga tcgaggaaca
    22021 gaccggtatc gaggtaatcc gtcagattca cccacaagtg agcgttatat tcggtaactt
    22081 ccagaaactc gcccttctcg cccagtttct ggtactgatt tttgcccttc tgacgttcac
    22141 gggttttcag caccagtttg tttggcgcaa tccccagtac cgaaatggtt gcagcgataa
    22201 tatcgaacag acgctgacgc gctttgtgag catcaatagt ttttggcggt gcatactcct
    22261 gcaccaccac ccagtcggca taacggtcaa cggcaacgtt atattccggc agatcggcgt
    22321 catacaggcg gtaacattca attccttcct gacgagccca cttctcgaat tttttgaggt
    22381 tcttacgcag gcggttggcg tagtcttccg ctaccatcgc cggtttgctg tctggagtgc
    22441 tttcggcaac gtggtaattt ttctgtacgc agtccagcgg gccgtttttc gccttgtact
    22501 gtttgtctgc acgcagctgc aagcagctta gcagatccgg cgaggcacta aacaaagaga
    22561 gattccagcc accaaactgg tttttcatga tccgacccag caggctatgc agcgcaatca
    22621 gcgccggttc gctgtccaga cgttccccgt atggcgggtt gctcaacact gtgccgtacg
    22681 gccctttcgg cagcggattg gtcagttgcg cgacatcttt cacctcaaag gtgatcagtt
    22741 caccaatccc cgcaagacgg gcgttagtgc gcgcacgttg aatcacccgt gcgtcgctgt
    22801 ccgaaccgta aaaatgagag ctatactcag ccaggccttt acgggcacga gtttgcgctt
    22861 ccgctttcac ttcctgccag atagcttcat catgctgcgc ccagccgcta aagccccaac
    22921 gcccacggtg caagcctggt gcgcgatcgg tcgccagcat cgccgcttca atcagcaacg
    22981 taccggaacc acacatcgga tcgagcagcg gtgttcctgg ctgccagccg gatcgcatca
    23041 caatagcggc tgccagggtt tctttgatcg gcgcaatacc agcacgatcg cgatagccac
    23101 gcagatgtaa accatcacca ctcagatcaa gagcgatact ggcggtttct ttatgcagcc
    23161 agacgttaac gcggatatcc ggcgcatcgc gatcaacatt tggacgcggc agatttttcc
    23221 gcgtgaaagc atcgacgatc gcgtctttca ctttcatcgc gccgtactga ctgttgcgga
    23281 tggtgtcatt caaaccactg aagtggacag cgaaggtcgc gccaggatta aacatctctg
    23341 tccagttgat cgcctgaaca ccgagataga ggtctaaatc gctgtaaacc ttacactcgc
    23401 ccagcggcaa cataatacgc gaggccaggc ggctccacat caggctctgg taaacaagcc
    23461 gtgtgtcgcc cttgaaatgg accccaccct gaaccacctg gcattcaacg gcccccaggt
    23521 tttccagttc agtttttaac agctcttcca gcccacgggc cgtactggca aacagagaat
    23581 tcatatcgtc acttttactc taagagccta ccgcagtagg cgtaattgtt gcagtcagtc
    23641 tgaacatgga cagcgcggag aaaccggagc gtacacaagt acgtgaggat ttcgagcaca
    23701 acccagggcc aaactgacaa ataaaatagc ctaatgggac aggcgctaag aaaattgttg
    23761 cgcattatag ctaataagcg cgtcatgtca taaagttgag ggcttatttt catttgagga
    23821 ccgcaccgtg gcgacattaa cccggctttt tattcatcct gttaaatcga tgcgcggcat
    23881 tggtcttaca catgctctgg cagatgacag tggtctggcc ttcgatcgca tctttatgat
    23941 tacggaacct gatggtacgt ttattaccgc tcgccagttt ccccagatgg tacggtttac
    24001 tccttcacct gtgcatgatg gcttgcatct caccgcacca gatggcagta gcgcatatgt
    24061 ccgttttgct gatttcgcca cacaagacgc accaaccgaa gtttggggca cacattttac
    24121 cgcgcgaatt gcgccagacg cgatcaacaa atggttaagt ggatttttct cccgcgaagt
    24181 gcaattacgc tgggtggggc cacaaatgac ccggcgcgtg aaacgccaca acactgtccc
    24241 cctgtcattt gctgatggct atccttacct tcttgctaac gaagcctcgt tacgtgatct
    24301 ccaacaacgt tgtccggcca gtgtaaaaat ggagcaattc cgccccaatc tggtggtttc
    24361 cggcgcgtca gcctgggaag aagatcgctg gaaagtgatt cgcattggtg atgtggtgtt
    24421 tgatgtggtt aaaccttgta gccgctgtat tttcaccacc gtcagcccag aaaaagggca
    24481 aaaacatccg gcaggcgaac cattaaaaac attgcaatct ttccgcactg cccaggataa
    24541 cggcgatgtc gattttggtc agaatttaat tgcccgtaat agcggcgtga ttcgcgttgg
    24601 cgatgaggtg gaaattctgg caacggctcc ggcaaaaatt tacggcgcag ctgccgctga
    24661 tgataccgcc aacatcacgc aacaaccgga cgccaatgta gatattgact ggcagggaca
    24721 ggcatttcgt ggaaataacc aacaagtgtt gctggagcaa ttagaaaatc agggaattcg
    24781 tatcccttat tcttgccgcg cgggcatttg tggaagttgc cgtgttcagc ttttagaagg
    24841 cgaagtcacg ccgctgaaaa aatcagcaat gggcgatgat ggcaccattc tttgctgtag
    24901 ctgtgtaccg aagactgccc ttaagctgac gcgttagact gcctgttcaa ggctgaagct
    24961 gtcaacatta acctgcggtt tcagcctgtc gttcatgatt ttaatggcgt cgcccaactg
    25021 catcgcgcga cccgcaataa caacgcaggg ttgcgccagc agacatagcg cggcattctc
    25081 gccagattca acgaccagta aattcacttg ctcgttagtg tcgctcaacc aaacgcaggc
    25141 cgcatcgccc tgcatgggca cccacatttc accatgggag acaaaatgcc agcttttcgg
    25201 catttgcggt ttgagataac gaattgctac cagtgcattc agcaccagtt cggctttttg
    25261 ttctttggat aacggcagat cacggcattt ttcttcaaaa gaaaaataga gtgctgcatc
    25321 atcaacacaa aaaccggagg gattaaaggc atctggtgtg agcattttac gagcaaaacg
    25381 cgagcgaaat aacataccat tggctaaatc gagcatcatt cgatcgtgct cttcatcata
    25441 ataccagcgc cagttatcgt caggtttaat tcgcatgcgg attctcccac ctaaatcatt
    25501 ctgcgcaaca ttgtcctttg ccgcttcgtt tattgcccta aataagaaaa gactaaaatg
    25561 tataaataag caacaggtaa ggaatataaa acaaccaggg ctggaaataa agccccggtt
    25621 gtctcataac gttaaaaagg agaaattaat cagatattgc taacgatttc tttaatcaaa
    25681 ggcggtcctt tgaaaataaa tcccgaataa atttgcacca gcgttgcacc cgcagcaatc
    25741 ttttcacgcg cagcgataac cgaatcgatg cctccaacac cgatgatcgg taagcgaccg
    25801 tttaattcct gtgacaagcg gcgaataatt tctgtgcttt ttaactgaag cggacgacca
    25861 cttaagccac cggtttgatc gcaatttttc attccctgaa ccagagaacg atcgagtgtg
    25921 gtattggttg caataacgcc atcaatatta tggcgaacta agctgtcagc aacctggatc
    25981 aattcttctt cagaaagatc cggcgcgatc ttcactgcga tcggcacata tttttgatgg
    26041 attttttgca gatcgttttg tttatttttg atcgcgatta ataagtcatc cagcgcttca
    26101 ccatattgca gagtgcgtaa tcccggtgta ttcggcgagg aaatattgat ggcgatataa
    26161 ccagcataag gataaatttt atccatacaa atcaggtagt catctttgcc ctgctccacc
    26221 ggcgtatctt tatttttgcc gatgttaata cccaggacgc cgtcataatg ggcttttttt
    26281 acattctcaa taagattatc caccccgaga ttgttaaagc ccattcgatt gatcagacct
    26341 tcggcatcaa ccaggcggaa aagacgcggc ttgtcatttc caggttgtgg acgcggcgtt
    26401 acagtgccga tctcaatcga gccaaacccc attgcaccaa gggcatcgat gcactccccg
    26461 tctttatcaa gaccggctgc cagaccaagc ggatttttaa acgtcaggcc catgcagtta
    26521 acaggtttcg caggcacttt ctgccgcacc agtgcttcaa acggcgttcc tgtaatacgg
    26581 cgtaattgct gaaaagtaaa ctcatgagcg cgctctggat cgagctggaa aagggcttta
    26641 cgaacgaagg ggtagtacat gaactctcct ggattcccgg tgtgcaaacc gggggcgtat
    26701 tatgtgcgag ttcggaccaa aagggaattg acctgcggca aaaaaaacgc aaacgttttc
    26761 cgaacctgtt tcagggcaga ttttgctcct tctttgcact caacccgccg taatcattta
    26821 tcgcccgcca ggcgacatga cacggtgcga ttgcctgaca ccagcttgtc tgacgctggc
    26881 ttttaggtgg cacaactccc gcattactta acgctttacc atttacgctc acctgaatca
    26941 ggttaatgta gtaaggcgtt gggttagtga gttgcacacc ctgcgaattc cgtgtccagc
    27001 gtaattgttg ataagcttcc agcgggtcgc ccggcaaacc ttcgggtcgc cagaacagtt
    27061 taaataccga gcgcatcgcc acttttacgc tctgattttc aactttgccg gatggcacgc
    27121 tggcaatgct taactcaaat agcgtttcgc gatccactgg caagatgtcg ctttccgttc
    27181 tcagcaagcg caacgtgccc gtagtaccgg gcttcaggag aataagcggt ggagcggcca
    27241 gtaatggtgc tggcactgtc gacacttccc ccccggccca acgcgttggg cgattgattt
    27301 tactgttaat aagccaggat tcctgactgg tattagtcag taaaatagct atcgattctt
    27361 tgtctgccgg aaagataaat cgcgttccgc cgaccaccac tcctgcctgc aattcacccg
    27421 ccaccagcag cgataaaacg gcaaaaatca agagcgccaa tctgttccac gtattagtca
    27481 taatgaagta caaatgtcgc gctggcattc actttacccg actgcacatc actattgaca
    27541 ggtcgtaatt gcgcataaaa agttaatggc accttaccgc tgtgtaggct gtagtcttta
    27601 ctcgcctggt taagcggaat caatatcttc gcatcatcca gaatcgcaat tgccagcccg
    27661 ctggctgcat ccataccact ttcaatggac agcatttcgc tctgcactcc gtcgggggtt
    27721 cctgaaaagg tgacgtcaac cgtgctgaca tctgttccgc aatcctgcaa attaatgatg
    27781 aaagatttct ccgggccata attcccgtga gaaaaacgtg tcagcgagac atcgcctaac
    27841 tgcacattga tttgcttgct atcctgtgcc agcacgcatg tattacgacg aaacgatccg
    27901 ctaatgttga ccgtgccatc atgagcaaat aactcactac tccccgttaa tcccagtaag
    27961 cacagaatcc agattatccg tttcaacatc actccttcac tggtaaacca gatcaaaata
    28021 gagaaccgcc gtcgcattac cgcccgttgc tcccgccttt gtggacttat aacgaagttg
    28081 atatttgagt gtgttatccc cggcttttaa gggcgtaaga ggctggacct gattgagcgg
    28141 gatttcttgc tgactttgcg catcaagaat ttgcacggca atccccgttg ccataccacc
    28201 gcttccgcct gtgtcagtta gtttgagcag tgtcggcgca agggtgtcag cctcgccgct
    28261 aaaaaggacg tccgcgccag taataccagc ggcacaaccg gtgatatcga tattcaaatc
    28321 agcagcagtg cttacggtat tcgcggcctg aaaatcactg atattaaaat caccaatatg
    28381 aatattttgt aatgcacttt tggtggcgac atcacagccc tgatcgacca cattcccata
    28441 aatatgcata ctaacttgtt caggcccagc catcccgaca tggatgttaa gaatgctaaa
    28501 gaacaagata acgcactgaa atatcgtttt tttcttcata cgttcacgcg ttaatcaaaa
    28561 tttattctca ccgttgccgt ggcttcaaat cgcccggttt ctggtggtac acctgtcgtg
    28621 ctgactggat agacattcat tcgcgcgata ctgtctgcat catcaggaat agagagcggt
    28681 aatgttccac cagcagggga aataatgttg ttctggctat cggtcaccac cacgcccacg
    28741 ccaggacgtg acgtcttaat cgcctgggga tagctggggt ctgtggttgc cgtcagactc
    28801 aggttgacgt taactaaccc cgcgtttccc gtgttacacc tgactggaat tgcgagttct
    28861 gccgttctgg ccccaagggg tttatgccct acaacacgaa agttagccgc aaatacgtcg
    28921 cccagatcga cctcgatagt ctctccggca ttgacggaac atgagccaag tgaattgatc
    28981 gtaccagaca ggtaatacac taaaaccgat gtacctgtag tcgtgcagga atcacctgcg
    29041 ggtatgttgt agcattcaaa taatcgcgcc agcgccatag gaggaatgac gaccgaacca
    29101 acgaacggct gcaatatttt cagggaaaag ctaccgctat taccgctagc cccctggcca
    29161 cctaaacgtt gaccgccggg atcgcaaata tgccatcgtg tatctgcccc tgtgcccacg
    29221 ttttcgaaag gaaccgcagt aggagaactg cttttaacct gcaataaaac tttggcggca
    29281 agatagtcat taattttgta ccagttgtcg cctaaataga ctaacggtga atccgcggcg
    29341 aatgcccatc ggccacttct gacattgtct ttatcgcaat cacaggaaac ataataatca
    29401 ttcgattcgt tccactgata aaatttgtct ttaactactc cagccgtatt ttcttcttcc
    29461 tccagggttt taacgccaaa attgacgcca atatacggat tcccctgagt ggtatgacac
    29521 tgcccgctat aagcagcagt ttgagcgcac catagcatta agacggcggc aaaaaatagt
    29581 cgtagtaata acacgcattt ttctccaaag attatctgca tatggcatcc attcgcgtaa
    29641 tcgcagagtt aatttgttgt tcggtaagct ggtaactgat atgacaaatt tgatcgggag
    29701 cttcgcccca gcgtacagtt atcgctccct gagcgggcaa accggacata tagagcgagc
    29761 catcatcatc aataatgccg ctattagtct gtatgccgtc cagcgtcgct atcgcgccaa
    29821 aacgcagcgg tataccctgc tttgatgttt tcattaatac ccttgctccc gcatgggcat
    29881 tgaactccgc cagaaccaac gcccctttgg tgggaaccac gttgactact gcattttcaa
    29941 gatccacgtt acgttttaac gatgccgcat caagtgcgat tcgattttcc cgataagaag
    30001 tggcataggg gacaacggta tatccgcgcc agtcagtttt cagcccggtg ctgttcgcca
    30061 cacgagtatt ctctgcgcct ggtgctgcaa tcaggacgtt agtttccccc agcgattgcc
    30121 ccagggtaat gccctgtgaa tgggcaacta aactgcctga aagagcgtag ttcagttgtt
    30181 gttgactgcc gttatcgctg tagttgtagc ccactcgggc atctgcaaac gcccctttgt
    30241 agtccatgct ggcgctacca ttagccgttt tcccctcgct gttatatccc tgttgcacgc
    30301 tgtagctcag gttaccgtca tcgagtagtg tctccgttaa acccaggttt tgtgtaatgt
    30361 gcccttcgtt atcgatactg gtgttagagg tcatccatgc gtaatttttt ttcctggtat
    30421 agctatcatt acttctgggg agcaacagat tgagcggcag ggaaaaattt agtgcaaaca
    30481 cctgatcagc atcaggttga ccacgggact tactgtagtt ccaggaaacc cccagcgaga
    30541 gatctttaat ttgcgtgttg tagccaaact gtaatagccg atcctgttga tcggtatgcc
    30601 agtaagtttg ctggctacca cttaaataaa acgaaccgta ctcgcctaat tgctgggaga
    30661 tattgacctg cagtttgcca cgtttggtgt aaaacaaatt gtaataacgc gaccacatcg
    30721 gcgtatcttc atcatcaccg tcattaaatt cgtagccatc catatgttta tacatggtgt
    30781 cggaaagggt gtagaaaccc gacgtcgaat agcgatagcc catcaattga aaattagtgc
    30841 ccaattggtt gagcgttttg gcatacagaa aacgatatga ctgaccttta tgttcgcttt
    30901 ggtctgccag cgtactcttc gcctgggtca catcgaacga aattgctccg aaatcgccaa
    30961 ggttaaatcc cagaccaaac atggcggcac ggtaatattc agcatattgt ccaccaccat
    31021 accatgtcgt tccccacggt ccgccccact gcaacgtggc ctgggcaaat ttgctctcct
    31081 gctgttcatt actattggtt ctgtatttcg ccagtgtcac cgcgtatttg attcgcccct
    31141 gacgctggag taatggcacg ctggaaaacg gtacgctgta gctattgacg ctgccgtccg
    31201 cttctttgat ttcaactaac aaatcaccgc tcgacgacgt ggaatagaga tcactaattt
    31261 caaaagcacc aggcgataca taagtttggt aaatggtgta accattttgc ttaatcgtta
    31321 tctgggcatt gcttttggcg ataccacgca ccacaggcgc aaaccctttc aggctgtctg
    31381 gcagcatgtt gtcatctgac gccagctgca caccacgaaa gctgacacta tcgaaaaaat
    31441 cgcctgcagt ataatcatca cctacggtca gttcgccttt tagtggaata accgcccgct
    31501 gcaaatagct gctgagattc ttccattctg cggtttggcc actactgcga ctccatgttg
    31561 aattgttgcg caatctccat gggcctaaat taacgccact gtttaaattc agaaaatagc
    31621 tgtcgccaga atcactgtct gcgctgctat gaatactgtt agccccgcta aacgaatatc
    31681 ccagtaatag cgcgttgatc ccttcatccc aacgttcagg ggggacataa ccacgggcat
    31741 tacttttgat ggcgatttgc ggtactgaaa tttccagacg gagtttattc acattaaacg
    31801 ttacgctggc atccggaata atctccgcca atggcacaca cgcttgtttg tccagttgtt
    31861 taaaagccgg aaacgcatca gtattaacac ccattgcatc caggctttca gtcgtcaaac
    31921 aggcggcaat ccccttctct ggcgttatct caacaaaatt gacgttgcgc gtatctacaa
    31981 ttgtctgatt cagaacaata tcgacacgat aaacccctgg cgttattttt tgtccttttt
    32041 cgaaacgaga caaatcggcc accattgagg gatcatcttt gaaaaaagca ggatcgaacc
    32101 aggtttcagc ttgtgcattg aacgctgccg acacaaacac caccagccca ccaataaacg
    32161 atggcactcc accagagctt aacaggctgt gttgtcgatg agttctatac atctcgctct
    32221 cttaactgaa gacggcttac gatttacagt tgagttgcgg cgtttccgcc ccgtagtcat
    32281 tgatggttcg atacgttacg ctgctatttt tggccccctt tggcagagaa aattggtatt
    32341 tatcaaaggg ggaaatcatt tgatttttaa ctacttttcc acctattttc aggtctgtca
    32401 gggtaatgaa ataaggtgtt gggttactga ccgtaaccat gtcagctgac gaagtgcaac
    32461 gcagtttcgc cggagcctcg cctaagggat acgctaaacc ttttggtcgc cagaaaagtt
    32521 tgatgacgct ttgtgtggca atcttcaggg tatttccctc ttcccgcttc gttgttgaag
    32581 ggactgcccg tacgctagtg aagaaaaggg tttctctgtc tttcgccaac ggcgcaccaa
    32641 tgtacataat gcgtaacaga ttctcattag ccggattcag cacaaatagc ggtggtgtaa
    32701 taataaaatc tgctgatttt ttattatctg gggtcatcac ccatgactga ataagataag
    32761 gaacatccgg actggtgtta cgtacaccca gtgaaatttg ctcttcacct tctgaataaa
    32821 caagacgtgt tgctgcaagt ccaatcccgc ctttacctgc cgcccatgcg gatgagcagc
    32881 tgagtaatat tgccgttaaa cttacggtca caattccctt tgttatgcaa gttttcataa
    32941 atagtcactc ttaaaaaata tgaatatccc ctccagaaca ctagaggggt actaattaat
    33001 aaaacaatta atgagttcta ttaataatag ttaatatgga aatttgttac cgcattcaca
    33061 ttaccagaag tcgctttccc cgtagcgata taatcaactt taaaggtcac tgtttgctca
    33121 gcagaatccg ctaactgaat attcgacgtt tcggtaccgg gagttaccga tgtagaacca
    33181 tctggcagat acatttgtag agcaacattc gtcgctgcat cggtggtagc ctggttggca
    33241 aatgctgtag gttgcacgcc gtcagccgta ccactaaagg taaacgtcgc atttttagtt
    33301 acagtaacat cacagccttc taaggcaatt ggtacatcag ttttaacgcc agagactgct
    33361 ccattagttt gcaacttggt ggcgctgaca tttggcaatg tgactactga atctttcgtc
    33421 gcagctacca gggtacaagc tggagcaata actttaccat taaatgttac tttgccatcc
    33481 gtgatagcat tatcatcagc tgccataacg ctggacgttg cacataccac agttataaac
    33541 gccgtcaata cacttttttt catcgtcatc atcctgatct cttcgtaaat atgataatgc
    33601 ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac
    33661 taaatccaat aagtgacaaa tctttaattt ataga
//
LOCUS       DAFAVF010000036        33068 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFAVF010000036 DAFAVF010000000
VERSION     DAFAVF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.036"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(124..264)
                     /locus_tag="LGK42_001696"
     CDS             complement(124..264)
                     /locus_tag="LGK42_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657956.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            360..1076
                     /gene="arcA"
                     /locus_tag="LGK42_001697"
     CDS             360..1076
                     /gene="arcA"
                     /locus_tag="LGK42_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBK2657957.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1136..2488)
                     /gene="creD"
                     /locus_tag="LGK42_001698"
     CDS             complement(1136..2488)
                     /gene="creD"
                     /locus_tag="LGK42_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBK2657958.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2546..3970)
                     /gene="creC"
                     /locus_tag="LGK42_001699"
     CDS             complement(2546..3970)
                     /gene="creC"
                     /locus_tag="LGK42_001699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBK2657959.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3970..4659)
                     /gene="creB"
                     /locus_tag="LGK42_001700"
     CDS             complement(3970..4659)
                     /gene="creB"
                     /locus_tag="LGK42_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBK2657960.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4672..5145)
                     /gene="creA"
                     /locus_tag="LGK42_001701"
     CDS             complement(4672..5145)
                     /gene="creA"
                     /locus_tag="LGK42_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBK2657961.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5356..6225
                     /gene="robA"
                     /locus_tag="LGK42_001702"
     CDS             5356..6225
                     /gene="robA"
                     /locus_tag="LGK42_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBK2657962.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6222..6869)
                     /gene="gpmB"
                     /locus_tag="LGK42_001703"
     CDS             complement(6222..6869)
                     /gene="gpmB"
                     /locus_tag="LGK42_001703"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBK2657963.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6921..7433
                     /gene="yjjX"
                     /locus_tag="LGK42_001704"
     CDS             6921..7433
                     /gene="yjjX"
                     /locus_tag="LGK42_001704"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBK2657964.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7475..7801)
                     /gene="trpR"
                     /locus_tag="LGK42_001705"
     CDS             complement(7475..7801)
                     /gene="trpR"
                     /locus_tag="LGK42_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBK2657965.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7891..9828)
                     /gene="sltY"
                     /locus_tag="LGK42_001706"
     CDS             complement(7891..9828)
                     /gene="sltY"
                     /locus_tag="LGK42_001706"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBK2657966.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10039..11706
                     /gene="ettA"
                     /locus_tag="LGK42_001707"
     CDS             10039..11706
                     /gene="ettA"
                     /locus_tag="LGK42_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBK2657967.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12013..13245)
                     /gene="nadR"
                     /locus_tag="LGK42_001708"
     CDS             complement(12013..13245)
                     /gene="nadR"
                     /locus_tag="LGK42_001708"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBK2657968.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13266..14648)
                     /gene="radA"
                     /locus_tag="LGK42_001709"
     CDS             complement(13266..14648)
                     /gene="radA"
                     /locus_tag="LGK42_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK2657969.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14697..15665)
                     /gene="serB"
                     /locus_tag="LGK42_001710"
     CDS             complement(14697..15665)
                     /gene="serB"
                     /locus_tag="LGK42_001710"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBK2657970.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15771..16415
                     /locus_tag="LGK42_001711"
     CDS             15771..16415
                     /locus_tag="LGK42_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBK2657971.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16443..17459
                     /gene="lplA"
                     /locus_tag="LGK42_001712"
     CDS             16443..17459
                     /gene="lplA"
                     /locus_tag="LGK42_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBK2657972.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17491..17640
                     /locus_tag="LGK42_001713"
     CDS             17491..17640
                     /locus_tag="LGK42_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2657973.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(17915..18634)
                     /gene="deoD"
                     /locus_tag="LGK42_001714"
     CDS             complement(17915..18634)
                     /gene="deoD"
                     /locus_tag="LGK42_001714"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK2657974.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18714..19937)
                     /gene="deoB"
                     /locus_tag="LGK42_001715"
     CDS             complement(18714..19937)
                     /gene="deoB"
                     /locus_tag="LGK42_001715"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK2657975.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19989..21311)
                     /gene="deoA"
                     /locus_tag="LGK42_001716"
     CDS             complement(19989..21311)
                     /gene="deoA"
                     /locus_tag="LGK42_001716"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBK2657976.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21438..22217)
                     /gene="deoC"
                     /locus_tag="LGK42_001717"
     CDS             complement(21438..22217)
                     /gene="deoC"
                     /locus_tag="LGK42_001717"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK2657977.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22475..24025
                     /locus_tag="LGK42_001718"
     CDS             22475..24025
                     /locus_tag="LGK42_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBK2657978.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23997..24860
                     /locus_tag="LGK42_001719"
     CDS             23997..24860
                     /locus_tag="LGK42_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBK2657979.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(24973..25755)
                     /locus_tag="LGK42_001720"
     CDS             complement(24973..25755)
                     /locus_tag="LGK42_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2657980.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(25752..26825)
                     /locus_tag="LGK42_001721"
     CDS             complement(25752..26825)
                     /locus_tag="LGK42_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBK2657981.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(26947..27108)
                     /locus_tag="LGK42_001722"
     CDS             complement(26947..27108)
                     /locus_tag="LGK42_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBK2657982.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27235..27840)
                     /gene="osmY"
                     /locus_tag="LGK42_001723"
     CDS             complement(27235..27840)
                     /gene="osmY"
                     /locus_tag="LGK42_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBK2657983.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28233..29822)
                     /gene="prfC"
                     /locus_tag="LGK42_001724"
     CDS             complement(28233..29822)
                     /gene="prfC"
                     /locus_tag="LGK42_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK2657984.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(29913..30590)
                     /gene="yjjG"
                     /locus_tag="LGK42_001725"
     CDS             complement(29913..30590)
                     /gene="yjjG"
                     /locus_tag="LGK42_001725"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBK2657985.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30605..31051)
                     /gene="rimI"
                     /locus_tag="LGK42_001726"
     CDS             complement(30605..31051)
                     /gene="rimI"
                     /locus_tag="LGK42_001726"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK2657986.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31020..31433)
                     /gene="holD"
                     /locus_tag="LGK42_001727"
     CDS             complement(31020..31433)
                     /gene="holD"
                     /locus_tag="LGK42_001727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBK2657987.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31536..32567
                     /gene="rsmC"
                     /locus_tag="LGK42_001728"
     CDS             31536..32567
                     /gene="rsmC"
                     /locus_tag="LGK42_001728"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBK2657988.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32835..32921
                     /locus_tag="LGK42_001729"
     tRNA            32835..32921
                     /locus_tag="LGK42_001729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32869..32871,aa:Leu,seq:cag)
     gene            32950..33036
                     /locus_tag="LGK42_001730"
     tRNA            32950..33036
                     /locus_tag="LGK42_001730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32984..32986,aa:Leu,seq:cag)
BASE COUNT         7778 a         9076 c         8461 g         7753 t
ORIGIN      
        1 ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac
       61 aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc
      121 ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg
      181 agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag
      241 aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag
      301 gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca
      361 tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa
      421 gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc
      481 agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga
      541 acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga
      601 ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca
      661 tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta
      721 ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg
      781 gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc
      841 tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt
      901 ctcgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccacac gaccgtactg
      961 tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa
     1021 tcatcgccac catccacggt gaaggttatc gcttctgtgg tgatctggaa gattaatcgg
     1081 ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt caaccttatt
     1141 tccagatacg taattgatcg tccattgtaa cttctttact ggctttcatt ttcggcagtg
     1201 aaaacgcata ccagtcgata ttacgggtca caaacatcat gccggccagc gccaccacca
     1261 gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc cacatcacac
     1321 catccagcaa caacagcgcg agggtaaaca acatgctgtt gcgccagcct ttcaataccg
     1381 cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc catgccacgg
     1441 taaaaccggt atgttcagaa agcgccagca agagcaaata aaacatcacc aatgaaagcc
     1501 ccaccagcaa atattgcatt gggtgtaagc gttgcgcggt gagcgtttca aaaacaaaga
     1561 acgccataaa agtcagcgca atcagcagaa tggcatactt agtcgcccgg tcagttaatt
     1621 ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt tcccagccgg
     1681 tatcattgcc tgaagcaaaa cgctcaccga gattatttgc aaaccagctg ctttgccatt
     1741 gcgcctgaaa acctgactcg ctaacttccc gtttggctgg caaaaaatca cctaaaaaac
     1801 tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca ggcaccacag
     1861 aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc tgcttgcgcc
     1921 agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac ccggtaccgg
     1981 gttcgatggt cagcgctgtt ccgttaactt caggcgcttt caccacacca ataccacgcg
     2041 catccccgac gctaatcaca ataaatggct tgcctaaggt gatatttggt gcgtcgagtt
     2101 cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc cagacctgac
     2161 cggtataaat ccctatcttg cgttcttcaa cgttctgatt gccatcaacc atcaatgact
     2221 caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcg tcttcctgca
     2281 ccgtataaag ctcggtcaca ggaatagcga tgagcggccc aacgagtttt tgcggcccgc
     2341 tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt tcgacaatca
     2401 cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac agggtggtca
     2461 ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca gcaggatacg
     2521 aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga agcgaggcca
     2581 gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct
     2641 cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc ggcaaagagt
     2701 aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg gtatccagca
     2761 ccttaagcgt gacgtgttcc tgatccactt cggcgcttag cgttatacga ccgctctcgg
     2821 gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgatccagt aacgccggtt
     2881 cagcagcaac attaacctcg gtgggcgtaa catgtagagt gatgtttttt tctgccaact
     2941 gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc cacatcaaca gcagtcaaaa
     3001 cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc agtgcctgca
     3061 ttcgcgcatt ttgcgtcaga atgttgtcag taaaacgagc cactacttca ggcggcggac
     3121 cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt agctcatggg
     3181 ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg cgcatacttt
     3241 ccagcgcctg cgcgagttta cgcagctcgc tactaccgag atcgggaaga ggaacgggct
     3301 tattgtcagt gacggaatca gcatagcgag tgagccgggc aatggagcgg ttgatccacc
     3361 aaaccatgcc tgcgccaatc accagtgcga tccccaacaa aatggcgctg gcccataaaa
     3421 ttcgccgctc gctgcgttta atgaccggag ccatcgccgc gttcggtttg cctacgctca
     3481 aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc accgaacttt
     3541 cgggatcggc aggattttgc aacgtgctgc gcgcaccata ctgaccacgc aacgttagcc
     3601 agacgtcatt ccagcgcgaa taatcctgcc caacggcttt atttgccgaa tcgaacaata
     3661 ctttaccctg cgaatcggtc atatagacat gatattcatt gcgcactttg ttaatgccac
     3721 cgatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc agttgcccat
     3781 gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac gttgcggtat
     3841 cgattaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg acaaaaatag
     3901 ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcagc aaccgcatgc
     3961 cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat taatcggtga
     4021 aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt ttaatgtggg tatcaacggt
     4081 gcgatcgtag gtgtcctgcg catcttccca cacgctatcc atcagttgct ggcgggacca
     4141 gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc gcgtcagcgt
     4201 taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa aatgaccaat
     4261 acggatgacg ggagacggcg acgagaactt cttcacccga cgcagtaagg tgcgcaccct
     4321 ggcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc caatttccag
     4381 cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag gtaacgccgg
     4441 atggagcgcc agtaactggc ggcataattc aaagccgctg atatcaggca aaccaacgtc
     4501 gagaatcatg acgtcgggaa ccagctggcg agctttatcc agcaccggca agcctcgctc
     4561 aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt cggctatccc
     4621 ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct gttattgccg
     4681 ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta caactttgtc
     4741 ggagtaagcc agataagcga gcgcgttgcg tttagcatca taaaagcgca cgacctgtaa
     4801 cgacttaaag accagggacg tacgtttttt gaatactacc tcgccctgag ctttgccgtt
     4861 tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatgg ccgcatcgga
     4921 ggtatcttcc gccaggccca atcccccttt aatacccccg gttttcgccc ggctcacata
     4981 acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt tgtgatccgg
     5041 gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag ccaatggccc
     5101 cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt taccattttt
     5161 atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa gaacaaaatc
     5221 tcaatacttt tatttccgtc aagccctaaa acatactcta ctaagaaaaa aacactgaat
     5281 gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc gttgttgaaa
     5341 ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat ctggctggaa
     5401 ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg ttattccaag
     5461 tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc gtatattcgt
     5521 gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc gattctggac
     5581 atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt caagaagcag
     5641 tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt tggtattcgc
     5701 ccgccgctac gtctgggtga attcacaatg ccagagcaca aatttgtcac cctggaagat
     5761 acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat ctctgatttc
     5821 cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc gaccattccg
     5881 ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga cgaacaagag
     5941 gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact gacggggcat
     6001 ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct gggaaccggc
     6061 gtgcaggagt ttatccttac ggtatacgga acgtgcatgc caatgctcaa cctgacgcgc
     6121 cgtaaaggtc aggatattga gcgatactac ccggcagaag acgccaaagc gggagatcgc
     6181 ccaattaatc tgcgctgtga actgctgatt ccgatccgtc gttaacgctg caactcatct
     6241 aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacaaccca gccagacgcc
     6301 agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag acgtaagcgg
     6361 cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag tgcaatacca
     6421 tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc cagtgccgca
     6481 ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc gtcaacggtg
     6541 ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc gatatgtctt
     6601 ttttccagca cacccatgtt taattcacgc aggcgagaat caaagatgat gtcacagccg
     6661 caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct actgatgata
     6721 tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg ctcacctttg
     6781 gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca ctgcgtttca
     6841 ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct gtaacgttaa
     6901 ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat cccgctaaaa
     6961 ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc catattgcat
     7021 ccgttgccgt cgagagcggt gtaccggaac aaccctttgg cagtgaggaa acgcgcgctg
     7081 gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc agaggctgat ttttgggtgg
     7141 cgattgaagc tggcatcgat ggtgacagca ctttcagttg ggtggtgatt gaaaacgcca
     7201 gccagcgcgg cgaggcgcgt tcggctactt tgccgttgcc tgcggtgatt ctgcagaaag
     7261 tgcgtgaagg tgaggcactt ggtcccgtaa tgtcgcgtta taccggtatt gatgagattg
     7321 gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc gccagcgtct
     7381 atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa tgcggtgtat taagcgcctg
     7441 atgcgacgct gacgcgtctt accaggctac aaaatcaatc gcttttcagc aacacctctt
     7501 ccagccactg gcgcagctcg acaggcgcgg ctttcaggct gttagatcca cgcgtaatcg
     7561 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg cctcgcagca
     7621 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
     7681 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
     7741 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
     7801 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
     7861 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
     7921 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
     7981 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca ctgcgtcgat
     8041 acgcccggcg ctgttgccaa gccaggttcg cacccgccct ggtccggcgt tataagctgc
     8101 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcagat aactggtgcc
     8161 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctactataac cgggaataga
     8221 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
     8281 taccggtgat ttcacttttg gattccaggc gctctcctga cgggcaatcg ccatcgcata
     8341 gctttgcggg atctccttac cgctggtgta gcgtttgaaa agatcgttgt aagccagcgg
     8401 gaatcgctct tccagatgat cccatagctt cccggcgatc gttgcctgaa cgctaagatc
     8461 ccaccattgg ttgttgaaag cataccgcgc cagttgagcc tgctctgttt ttgacttgct
     8521 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
     8581 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
     8641 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
     8701 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
     8761 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
     8821 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
     8881 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
     8941 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
     9001 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
     9061 atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa aatcggtcgc
     9121 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
     9181 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
     9241 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
     9301 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
     9361 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
     9421 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
     9481 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
     9541 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
     9601 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
     9661 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
     9721 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
     9781 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
     9841 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
     9901 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
     9961 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    10021 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    10081 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    10141 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    10201 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    10261 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    10321 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    10381 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    10441 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct gcgtctgcca
    10501 gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    10561 cgcctgcttc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    10621 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgttgtggcg
    10681 attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    10741 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    10801 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    10861 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    10921 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    10981 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    11041 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    11101 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    11161 ccggacagcg gcaccatcac tttaggtgaa acggtaaaac tggcatcggt tgatcagttc
    11221 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    11281 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    11341 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    11401 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaatgac
    11461 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgcgcg
    11521 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat tctggattac
    11581 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ccgagtacga agagtacaag
    11641 aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    11701 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    11761 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    11821 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    11881 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    11941 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    12001 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    12061 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    12121 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    12181 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    12241 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    12301 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    12361 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    12421 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    12481 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    12541 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    12601 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    12661 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    12721 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    12781 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    12841 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    12901 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    12961 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    13021 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    13081 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    13141 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    13201 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    13261 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    13321 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    13381 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    13441 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    13501 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    13561 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    13621 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    13681 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    13741 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    13801 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    13861 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    13921 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    13981 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    14041 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    14101 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    14161 cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagt
    14221 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc
    14281 atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc cgcaccaggg
    14341 ttaccgccaa tcagaatggc acttcctggc accacgccgc cgcctagtac gcggtcgaac
    14401 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    14461 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    14521 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    14581 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    14641 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    14701 ttctgattca ggctgcctga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    14761 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    14821 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    14881 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    14941 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    15001 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    15061 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    15121 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    15181 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    15241 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    15301 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    15361 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    15421 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    15481 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    15541 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    15601 gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    15661 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    15721 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    15781 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    15841 tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac
    15901 agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga
    15961 tgcgtaccga ctcaccagat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    16021 aaagccgcat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcccgttcag
    16081 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagcg ggcggctatt
    16141 ttaaccagca gattgtcgaa ccaattgccg ggaaaaacgg accgctcggc tatctgcgcc
    16201 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    16261 ttttacgcct gatgttgctg ctttcactgg caatcggcgt agtgctgacc cgcacgctgc
    16321 tacagggtaa acgtacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    16381 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    16441 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    16501 tggaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    16561 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    16621 ggatggaaga agataacgtc cgcctggcac ggcgcagtag cggtggcggc gcggtgttcc
    16681 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    16741 tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    16801 ggcgtaacga tctggtggtg aaaaccgccg aaggtgaccg caaagtctca ggatcggcct
    16861 atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat gccgacctta
    16921 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    16981 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    17041 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    17101 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    17161 gtagctggga atggaacttc ggtcaggctc ctgcattctc gcatctgctg gatgaacgct
    17221 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    17281 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    17341 gcggcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    17401 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    17461 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    17521 attatcaata taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    17581 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    17641 atcgaaaaga cggaacggtt tagctcaaac atcaaaagca aaatggcaga aatttatggt
    17701 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    17761 tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata atagtaaatc
    17821 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct cccactccgg
    17881 cttcacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    17941 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    18001 gctcgtgagt gcggatgtgg tcagacacgg tacagatggt cagggctttc gcgccaaatt
    18061 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    18121 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    18181 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    18241 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    18301 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    18361 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    18421 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttataag
    18481 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    18541 tttcagcaat atactttgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    18601 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatct acatgttttc
    18661 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    18721 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    18781 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    18841 accggaatgt gttcacgcgt gtgatcagta ccggtccagg tcggatcaca accgtggtca
    18901 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    18961 tcgaacagtt ccagacccgc cgcataacct gcgacgtcgc gacggtggcc ccaggaagag
    19021 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    19081 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    19141 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    19201 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    19261 cgctggaagt taccggcttt gtcgccaata aacggacgag cgataacacg accgatgttg
    19321 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaag
    19381 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    19441 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    19501 tggcagttac cgagataacc aggcaggtta gcgcgttcaa ccagtttatc cagcagctct
    19561 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    19621 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    19681 tacgcgccga taacttcagc gttgccgtcc attcctgccg gaatgaaacc ggtagaacct
    19741 tcatgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    19801 ccgttatcag cttcgccttt ggcacaggct tctgcgatat gacccagggt gtcagccccg
    19861 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    19921 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    19981 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    20041 gcaagtttaa ttgccgcttt caccgctttc gccgcgtcct gccagctgtt ttcgtctttc
    20101 gcgtggataa cagccagcgg acgctgaccg tctacctggt cgcccaaacg cgccatatca
    20161 gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc gcccattgca
    20221 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    20281 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    20341 gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    20401 agcactgctt gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagata
    20461 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattccccc
    20521 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    20581 tgattcatat cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    20641 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    20701 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    20761 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    20821 tccgccggag ccagtgagct ggtctggccg ataatcgcta cgccgacgtc tttaataatt
    20881 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    20941 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    21001 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtggagtgt
    21061 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    21121 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    21181 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag
    21241 aaacgaattt cttcatcgct tagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    21301 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    21361 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    21421 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    21481 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    21541 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    21601 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    21661 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    21721 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    21781 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    21841 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    21901 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    21961 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    22021 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    22081 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    22141 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    22201 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    22261 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    22321 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    22381 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    22441 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    22501 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    22561 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    22621 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    22681 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    22741 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    22801 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    22861 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    22921 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    22981 tacccgtgca atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    23041 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    23101 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    23161 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    23221 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    23281 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    23341 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    23401 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    23461 gtgtgagaaa gaagggattg ccgcacgtta cggtaaagaa gccgccgcaa atgaagtggg
    23521 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggatg
    23581 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatattg gcaccacgcc
    23641 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    23701 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    23761 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    23821 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtacg
    23881 tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    23941 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    24001 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggta
    24061 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    24121 ggacgatggg acgctgcaat gattgcggag agtgcgtgcc acagtgtccg catcaggcgt
    24181 tgcagattgt tgacggcaaa gtgctgtgga acgctgtggt ctgcgagcag tgtgatacct
    24241 gcctgaagat gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    24301 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    24361 aagccacgac ccaactgccg tttgtagtgg cgctatttac agccatcaaa aacgatccgc
    24421 aactgcgcca cctcacctgc ctcgtggaca gtaacggtat gttgagcgaa accggctggg
    24481 aaaaattact cccggtgtgc gacggcgcga tgctcgatct caaagcgtgg gggagcgaat
    24541 gtcatcagca attaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgctgg
    24601 cagagcgcgg taagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    24661 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    24721 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    24781 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    24841 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca aacctgtagg
    24901 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgctgcct
    24961 acgaagggta aactacccac gaacgctaaa caccgcatac gtattattaa gcaacacttc
    25021 ggcaatctca tcttctggtt ccggacgtaa ctcgcaaagc acgtcaaaca cccgcgcagc
    25081 ctgttccggg cggttaggct tcccctgaaa gccgttgagc ggcatatccg gcgcgtcggt
    25141 ttccagcaac aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg
    25201 tggataggtg atagtaccgc ctacgccgat tttgtagccc agctgtacaa accgctcggc
    25261 ctgttgcagg ctgccggaaa aaccgtgaac cacgccagtg cgcgataaat cgtggcgttt
    25321 aagatgcatc gccagtttgt cgtgtgtgcg ccgtgaatgc aggatcaccg gcagatcgta
    25381 gcgtttcgcc agtttcagct gttcgtcgag taaccactgt tgcctctcaa attgcggatc
    25441 gtcgccgaag agatccagac cgatctcccc caccgccacc accttcgccg gacgcctttc
    25501 cagcgcctgc tgtagctgat caagagacac atcgctatgt ttttccaaca tccccggatg
    25561 caaccccaat gcggcataca gcggttgata tttttccgct aacgcctgca cgcgggcaaa
    25621 attttccgcc tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg
    25681 caggctggcc tcttcatcgc cactaaacgg cgggaaatca aaatggcagt gggtatcgat
    25741 aaaacggcaa atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagca
    25801 tcgctcactt cggcaaccag cgtatcgttg gcgactggcg caggcggaat aacgatcgat
    25861 tccggcgtca ccactggcac cagatggcgg gtaagcggcg ctttttcagt taatagcttg
    25921 ccaaccgtgg cgaggaaata acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc
    25981 gccggaatcc gactgccaag cgcgatacta tgtaatggct tcggtggata aatttcgaat
    26041 atccgcagct tgccaggtgg tttctcaata aattgctgaa tgtcacgata gctggtttca
    26101 tgatgctgca ccagattgac cagcggctgc aggctactgt cacccagcca gcgttccatg
    26161 cgtttgaacc actgcggcgt gtagtacatt tgtgacggca cagtgcggat gacgaccaac
    26221 gttttagcgc cctgccttgc cgcctcttta accggaatcg catcactgat cccgccatcc
    26281 aggtagttaa tgccttccag cgacactccg ctacgataaa agccaggtat cgcactggag
    26341 gcgcgaatca catccagcca gttttgtttg gttggtaaaa agtaattcgg cgcgtagtca
    26401 tcctgacgac aggcgcacat ataaaacgat ttgccgctgt caaacaaccg cgcggcggta
    26461 tccatttgca acggcatctg acttgcggtg gcctccacca gccagtcgag atcgataaga
    26521 tttcctccac ggacaaagcg caatggatcg aaaaattcgc gttttgtggt atagcgcatg
    26581 atgactttgc gcgcgtaacc gggctgattg cagatatacg ccgagaggtt ctgcgccccg
    26641 gcagatgtgc cgagataaag atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc
    26701 acgccagccg tgaaaattcc acgctgtccg ccgccttcgc acaccagagc tattcgtcca
    26761 ggctggaacg gccttagcga caacggcgca atattaccaa gcgttacagg tattcgctgc
    26821 cccacctctg ccttcctgtt tttgttgtga aagaatgaca aagtaacgca atttattatt
    26881 cgctaaaacc gcaaaagcca gtccgctgga ctggctttgg ataaaatttt cttaatggtc
    26941 tgaaatctag ggtcgttttc ggcccatgaa caaactcacc aggaacagaa taatcccgac
    27001 gacaaagaca attttagctg cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc
    27061 ggcggcgatt aacgcgataa ccagaaatat gatgccccaa cgaaacataa gcgtctcctt
    27121 taccatagtt aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc gatattttta
    27181 gtgtggtgca catcgcgcct cccgacaaag ttcgggagga cgaattacga cgaattactt
    27241 agttttcaga tcatttttaa cgcttttcac accatctacc gctttggcga tactttcagc
    27301 acggtcgctc tgtgcctggg aatcgacggt accggagagc tgaaccacgc cgtcggtggt
    27361 ttcaactttc acatgacggg aagggacgat atcgtccgcc agcagtttgg ctttgatttc
    27421 actggtggtg gcggtgtcac ccgcgtagcc cttcaccgag ccttctttag cgtcgcgaac
    27481 gtgcagtttg tcgctgacag aggtcacccc ttcaacgcct ttcgccactt tcactgcctc
    27541 ttcggcctga gcctggcttt caacgaaacc gctcagggtc acgacttttt gatcggtttt
    27601 gacagagata tcggtgctct tgatgttgtc atgatccacc agcgccgcct tcactttcgc
    27661 ggtgatggcg ctgtcatcca tgaaattacc gactttattc atagagctat cgactttttg
    27721 ccctgcgctt tcattggtag tctgtgcgtt gttttccgcg taggcagagc cggtcgcgac
    27781 ggcagaggtc aacattacag ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat
    27841 cgatttattc ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt tgcattgctg
    27901 aatagggaga gacttcaccc tctacagaag tcaatggtcg ccatcacaaa agcgatgggt
    27961 gatgaataac gaccattaca gcctctgaat cagttattaa tatcggtaga atgacaatcg
    28021 acggctttag atactgatat ctacgcattg aacggtattt aacgccgtca gaaatgtcat
    28081 tactttgtta aatatagttc acaattttga aaccgctcgg gatatcagcg aaaacataag
    28141 caaaagtgaa tgttttaaga acattccgta agcggctaat aaggaaggga aattgacagg
    28201 gcgcagcggc taccgcgccc tggaggcaag aattaatgct cgcgggtctg gtggaactga
    28261 acgtcgggat aacgttcctg cgccaggcgc aggttgacca tgctggtagc gatgtaagcg
    28321 aggttatcgc cgccatcaag cgccagttgg ctttcgttct tacgcttgaa ctcttcgaat
    28381 ttcttcgcgt ccgcacattc tacccagcgg gcagtggcaa cgttgactga ttcatatact
    28441 gcttcaacgt tgtattcgct cttcaggcgc gataccacca catcaaactg cagcacacca
    28501 acagcaccaa cgatcaaatc gttgttggag atcggacgga acacctgcac cgcgccctct
    28561 tcggaaagct gtaccagccc tttgagcagc tgtttttgct tcagcggatc tttcaggcgg
    28621 atacgacgga acagttctgg tgcgaagttc ggaataccgg tgaacttcat catctcaccc
    28681 tgggtaaagg tgtcgccgat ctgaatagtg ccgtggttgt gcaggccgag gatatcgccc
    28741 ggatacgctt cttcaacgtg cgaacggtcg cccgccataa aggtcagcgc gtcggagata
    28801 accacatctt tcgcagtacg cacctggcgc agcttcatgc ctttttcata tttaccggat
    28861 actacgcgca taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc ctgaatttta
    28921 aatacgaagc cggtaaattt gtcttcgctc gcctctacgg tacgggtatc agtctgacgc
    28981 ggcatcggcg caggagccca ctccaccagg ccatccagca tatgatcaac accgaagtta
    29041 cccagcgcag taccgaagaa taccggagtg atttcgcccg caaggaacag ttctttgtcg
    29101 aactcgttag acgcgccttt caccagttcc agttcgtcac gtagctgctg tgccagatct
    29161 tcaccaaccg cagcgtcgag atccgggtta ttcagccctt taacaatacg gacttcctga
    29221 atcgtgtggc ctttaccgct ctgatagaga taggtttcgt ctttataaag gtggtaaacg
    29281 cctttaaaca gcttgccgca gccaatcggc caggtgatcg gtgcgcagcc gattttcagc
    29341 tcgttctcaa cttcatcgag cagttccatc gggtcgcgga tatcacggtc aagtttgttc
    29401 ataaaggtga ggatcggcgt gtcgcgcaga cgggtaactt ccatcagctt acgggtacga
    29461 tcttcaacac cttttgcagc gtcgataacc atcaggcagc agtccaccgc cgtcagggta
    29521 cgataggtat cttccgagaa gtcttcgtgc cccggggtgt cgagcaggtt aaccaggcaa
    29581 tcgtgatacg gaaactgcat cacagacgta gtaatggaga tcccacgctg cttttccatc
    29641 tccatccagt ccgacttagc gtgctggttg gaaccacggc cttttactgt accggcggtc
    29701 tgaatggcct gtccgaacag cagcaccttc tcggtgatgg tagtcttacc ggcatccggg
    29761 tgagaaataa tggcaaaagt gcggcgcttc gccacctctt gcaaataagg agacaacgtc
    29821 ataattcttt cttcttgagt aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc
    29881 ggctatttta cccatcaacg ggggggaggc aatcagtgtt tacacaggag ctgctccagt
    29941 tcgtgcaacg aagaaacggt ccaggtgggc gcgatgcctt ctggttgctc gcgatggtgc
    30001 gcattcagcc agcaggtcgc aagcccggcg ttgatgccac cgagaatatc ggactcggca
    30061 gtgtcgccaa ccatcagcac gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa
    30121 tcgaaaattt tcttattcgg tttggcaacg ccaacttctt cggaaatcac cagcaaatcg
    30181 aagtaatcac gcaggcccgt gcgttccaga cgcacttgct gcaaggcgct aaagccgttg
    30241 gtgatgatgc cgattttggc gttgccacga atggcgttaa gcagagaaac cgcgcccggc
    30301 agcggcgtgc agatttccgc catcgcatta ataaaggcat cgttaagcaa gcctgcttca
    30361 actttcagcc gttcggccca gctctcaaaa cgcccgtgct gaagctgtaa tgaagtgatc
    30421 gcgccgtttt gataatccac ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct
    30481 tcagcggtaa aggtgacgct gtaatcaaga aacatccgct gcaggccggt gaatgagtca
    30541 aaggtaaaca gcgtttcatc ggcatcaaag aaaatccagt cccacttcat tattccacct
    30601 tgtcttacat actgattggc aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg
    30661 ggtagtaatt gcggcgaatc gtcgcctcgt taaagcctaa actttcgtac agggcaatgg
    30721 cggcagcatt tgaggcacgg acttccagcc atagtgtcgc cacgccgcgt ttttccagtt
    30781 cgtcgatcag atgttccagc agcacccttc ccaatccctg acgctgatag tcaggatcga
    30841 ccgcaatatt gaacaatgta gcttcatcca gcaccacttg cgtaatcgca aacgccgcca
    30901 ttttgccgtt ttgcgttaac tgaaagttga gataacgctc gccctggttg ctggcaaagg
    30961 ttttttcact ccacggaaag gcgtgggcgc gttgttcaat gtggtaagcc gctggtaaat
    31021 cagtcgtttc gagggaagaa atcgtgttca tatgtgcaaa tttgttgcca taacgcggcg
    31081 cgtgccgttg ggtttgcccg taattcggtg agcgccggtg atgccacctg agcgccttcc
    31141 agtgatagcg gttcgtcagt acccaaccgc cagctgttgc agcgactgcc ttgcggcagc
    31201 atcgcgattt tttctggcgt cagttgcagc acttgatcgg ggctgacggt taatgcgcgc
    31261 agaacatcgc tcactaaagg atcggtcagg gcgggaagat cgtttgccac catcaccaga
    31321 cggacgtgtg ccgggatcgc aatggcaatc tccccctgca acgcgccagg gcgacgcagc
    31381 gaccactggg taatgcccag ttgctgtaac tgccagtctc gtcgggatgt catagcgaat
    31441 cgctcctgtt gtcaggggcg caaatatagc aaattcgtcg ataccgcgcc aacatatggc
    31501 tataatcgcc gccagtatca attgaggagc attccatgtc tgcatttacc ccggcaagtg
    31561 aagtcttgct gcgtcacagt gatgatttcg aacaaagccg tattctgttt gccggagact
    31621 tacaggatga cctgcccgcg cgtttagata ccgcggccag ccgtgctcat acccagcaat
    31681 tccaccactg gcaggtatta agccgccaga tgggggataa cgcccgtttc agtctggtcg
    31741 ccacggcgga tgacgtcgca gattgcgata cgctgattta ctactggccg aagaacaaac
    31801 cggaagccca gttccagttg atgaatttac tttctctgct gccggtgggg acggatattt
    31861 ttgtcgttgg cgagaaccgc agcggcgtgc gcagcgccga gcagatgctg gcagattatg
    31921 cgccgttgaa taaagtcgac agcgctcgtc gctgtggcct ctattttggt cgtctggaaa
    31981 aacagccggt atttgatgcc gataaattct ggggcgaata cagcgtcgat ggcctgacgg
    32041 tcaaaacgct gcctggcgtg tttagccgcg acggtctgga tgtcggtagc cagttgctgc
    32101 tctcgacgtt aactccgcac acgaaaggta aagtgctgga tgtcggctgt ggcgcggggg
    32161 tgctttcagt tgcctttgcg cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct
    32221 ctgcgccagc ggtagaagcc agccgcgcaa cacttgcggc caactgtgtt gaaggtgaag
    32281 tctttgccag caacgtcttt tccgaggtga aaggtcgttt tgatatgatc atctccaacc
    32341 cgccgttcca cgatgggatg caaaccagcc tggatgcggc gcaaacgctg attcgcggcg
    32401 cggtgcgtca tcttaatagc ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt
    32461 acccggacgt gctggatgag acatttggct tccatgaagt gattgcgcaa accgggcgtt
    32521 tcaaggtgta tcgcgccatt atgacccgcc aggcgaagaa aggttaatta tctcgccgaa
    32581 taccgtgtcg gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgttagccct
    32641 tcgtaggcca gataagacgc gccagcgtcg catctggcat ctgcccccgc atgcctacca
    32701 aacctgcacg ctagtttcct gatggacatt tttccagcaa ttacacctcc ggcgataatt
    32761 aactattgac gaaaagctga aaaccactag aatgcgcctc cgtggtagca attcttttta
    32821 agaattgatg gtatgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg
    32881 tccttacgga cgtgggggtt caagtccccc ccctcgcacc aaaaaccacg ttgatattgc
    32941 tcgcactggg cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgttctt
    33001 acggacgtgg gggttcaagt cccccccctc gcaccaaacg aggcgatatc aaaaaaagta
    33061 agatgact
//
LOCUS       DAFAVF010000037        32653 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFAVF010000037 DAFAVF010000000
VERSION     DAFAVF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.037"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(226..354)
                     /locus_tag="LGK42_001731"
                     /pseudo
     CDS             complement(226..354)
                     /locus_tag="LGK42_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            426..608
                     /locus_tag="LGK42_001732"
     CDS             426..608
                     /locus_tag="LGK42_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBK2657989.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(709..1038)
                     /locus_tag="LGK42_001733"
     CDS             complement(709..1038)
                     /locus_tag="LGK42_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBK2657990.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1210..2268)
                     /locus_tag="LGK42_001734"
     CDS             complement(1210..2268)
                     /locus_tag="LGK42_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2657991.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2466..2939)
                     /gene="sbmC"
                     /locus_tag="LGK42_001735"
     CDS             complement(2466..2939)
                     /gene="sbmC"
                     /locus_tag="LGK42_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBK2657992.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3058..4224)
                     /gene="dacD"
                     /locus_tag="LGK42_001736"
     CDS             complement(3058..4224)
                     /gene="dacD"
                     /locus_tag="LGK42_001736"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBK2657993.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4433..5860
                     /gene="sbcB"
                     /locus_tag="LGK42_001737"
     CDS             4433..5860
                     /gene="sbcB"
                     /locus_tag="LGK42_001737"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBK2657994.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLNNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(5903..6130)
                     /locus_tag="LGK42_001738"
     CDS             complement(5903..6130)
                     /locus_tag="LGK42_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBK2657995.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6144..7202)
                     /gene="yeeE"
                     /locus_tag="LGK42_001739"
     CDS             complement(6144..7202)
                     /gene="yeeE"
                     /locus_tag="LGK42_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBK2657996.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIVISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(7381..8739)
                     /gene="plaP"
                     /locus_tag="LGK42_001740"
     CDS             complement(7381..8739)
                     /gene="plaP"
                     /locus_tag="LGK42_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBK2657997.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(8729..8791)
                     /gene="yoeI"
                     /locus_tag="LGK42_001741"
     CDS             complement(8729..8791)
                     /gene="yoeI"
                     /locus_tag="LGK42_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBK2657998.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9006..9935)
                     /locus_tag="LGK42_001742"
     CDS             complement(9006..9935)
                     /locus_tag="LGK42_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2657999.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(9981..10805)
                     /locus_tag="LGK42_001743"
     CDS             complement(9981..10805)
                     /locus_tag="LGK42_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK2658000.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            11231..11281
                     /gene="hisL"
                     /locus_tag="LGK42_001744"
     CDS             11231..11281
                     /gene="hisL"
                     /locus_tag="LGK42_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBK2658001.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    11257..11380
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            11427..12326
                     /gene="hisG"
                     /locus_tag="LGK42_001745"
     CDS             11427..12326
                     /gene="hisG"
                     /locus_tag="LGK42_001745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBK2658002.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            12332..13636
                     /gene="hisD"
                     /locus_tag="LGK42_001746"
     CDS             12332..13636
                     /gene="hisD"
                     /locus_tag="LGK42_001746"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBK2658003.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDMVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            13633..14703
                     /gene="hisC"
                     /locus_tag="LGK42_001747"
     CDS             13633..14703
                     /gene="hisC"
                     /locus_tag="LGK42_001747"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBK2658004.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQLSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            14703..15770
                     /gene="hisB"
                     /locus_tag="LGK42_001748"
     CDS             14703..15770
                     /gene="hisB"
                     /locus_tag="LGK42_001748"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBK2658005.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRADSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            15770..16360
                     /gene="hisH"
                     /locus_tag="LGK42_001749"
     CDS             15770..16360
                     /gene="hisH"
                     /locus_tag="LGK42_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBK2658006.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            16360..17097
                     /gene="hisA"
                     /locus_tag="LGK42_001750"
     CDS             16360..17097
                     /gene="hisA"
                     /locus_tag="LGK42_001750"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBK2658007.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            17079..17855
                     /gene="hisF"
                     /locus_tag="LGK42_001751"
     CDS             17079..17855
                     /gene="hisF"
                     /locus_tag="LGK42_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBK2658008.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            17849..18457
                     /locus_tag="LGK42_001752"
     CDS             17849..18457
                     /locus_tag="LGK42_001752"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBK2658009.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELKNEASDLVYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(18496..19611)
                     /locus_tag="LGK42_001753"
     CDS             complement(18496..19611)
                     /locus_tag="LGK42_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK2658010.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KDTGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKLIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLSGVDCIVASSPNYVASSQTLKKY
                     LDKTVVIPFGLEQQDVQHDPQRVAHWRETVGDKFFLFVGTFRYYKGLHILMDAAERSR
                     LPVVVVGGGPLESEVRREAQQRGLSNVMFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(19621..20766)
                     /locus_tag="LGK42_001754"
     CDS             complement(19621..20766)
                     /locus_tag="LGK42_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK2658011.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIEQIPLVENKSDTKASNHGRLSAFLRRQTLLIEAYRLLHPRRQAWALRDYKDYIYHG
                     PNFYLPHKLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSR
                     SEIIRLFNYPAERIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQAYALYIGTMEPRK
                     NIRGLLHAYQLLPMEIRMRYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDED
                     LPYLYAAARVFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDI
                     DAISAQILQSLQDDSWREIATARGLAQAKQFSWENCATQTINAYKLL"
     gene            complement(20830..24471)
                     /locus_tag="LGK42_001755"
     CDS             complement(20830..24471)
                     /locus_tag="LGK42_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK2658012.1"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIANIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVAASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIGNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKEMAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAISQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFDSSYSLALVNREFARALSADGVEVLLHSTEGPGDFAPDAS
                     FMAQSENSDLLAFYNQCQTRKSNEKIDIISRNIYPPRVTKMDAKVKFLHCYAWEETGF
                     PQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHWLNIPAETTKDVDH
                     GTFRFLHVSSCFPRKGIQAMLQAWGKAFTRRDNVILIIKTFNNPHNEIDAWLAQAQAQ
                     FIDYPKVEVIKEDMSATELKGLYESCDVLVAPGCAEGFGLPIAEAMLSGLPAIVTNWS
                     GQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLTDALKAAASTDKSVLRDM
                     ANAGRELLLQQFTWKAVADRSCQAVKTLRAHIDIAQHRARIGWVTTWNTKCGIATYSQ
                     HLVESAPHGADVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLENLQPHIDALRLDV
                     IVIQFNYGFFNHRELSAFIRRQHDAGRSVVMTMHSTVDPLEKEPSWNFRLAEMKEALA
                     LCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQSLPLIASYGFCLP
                     HKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAAAQRLGVTDLIEMH
                     NDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVAVTPLAIFDDLDDA
                     VFKFDGCSVDDISQGIDRILNSIREQNSWATRTQQRADAWREQHDYQAVSRRLVNMCQ
                     GLAKAKYFK"
     gene            complement(24474..25751)
                     /locus_tag="LGK42_001756"
     CDS             complement(24474..25751)
                     /locus_tag="LGK42_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK2658013.1"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGVIA
                     DIGCGRGEWLEILTENGIANIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLAMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFTRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIDQNHQLHQQMADLHNSRSWRITQPLRWLSLQRQLLRQEGAKV
                     RARRAGKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     MMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     gene            complement(25751..26965)
                     /locus_tag="LGK42_001757"
     CDS             complement(25751..26965)
                     /locus_tag="LGK42_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter ATP-binding
                     protein Wzt"
                     /protein_id="HBK2658014.1"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILRDI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTISEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     gene            complement(26965..27759)
                     /locus_tag="LGK42_001758"
     CDS             complement(26965..27759)
                     /locus_tag="LGK42_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter permease
                     subunit Wzm"
                     /protein_id="HBK2658015.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     gene            complement(27761..29137)
                     /gene="rfbK2"
                     /locus_tag="LGK42_001759"
     CDS             complement(27761..29137)
                     /gene="rfbK2"
                     /locus_tag="LGK42_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK2"
                     /protein_id="HBK2658016.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFSNFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQKAFPASGEINRKLSNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(29161..30576)
                     /locus_tag="LGK42_001760"
     CDS             complement(29161..30576)
                     /locus_tag="LGK42_001760"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HBK2658017.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRDG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     EACQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(30858..31142)
                     /locus_tag="LGK42_001761"
     CDS             complement(30858..31142)
                     /locus_tag="LGK42_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658018.1"
                     /translation="MSRLSHQSQHNAVVVCKELLSPNARCLPGGEYDVSSYLSPYRKC
                     PFLIVAGIGKSLDSFFLMAVRACVLPRVNEKVLMVTLAANVEIVGRFVAW"
     gene            31387..31533
                     /locus_tag="LGK42_001762"
     CDS             31387..31533
                     /locus_tag="LGK42_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658019.1"
                     /translation="MLAVSNPVLRRYILTNEVPDLMYHLLVLLQVQNLDLTTVIVNLR
                     TRHQ"
     gene            complement(31727..32653)
                     /gene="wzzB"
                     /locus_tag="LGK42_001763"
     CDS             complement(31727..32653)
                     /gene="wzzB"
                     /locus_tag="LGK42_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBK2658020.1"
                     /translation="MDLIDLLVQLWRDKMTIIISVIVAIALAIGYLVVAKEKWTSTAI
                     VTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSILIGRFSTTFSALAETLDNQEEPEKL
                     TIEPTVKNQSLPLAVSYVGQSPEAAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKN
                     LQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSDA
                     LESMVKHEASRPLVFSSTYYQTRQNLLDIESLKVDDLDIHAYRYVMKPTLPIRRDSPK
                     KAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         8444 a         8605 c         7961 g         7643 t
ORIGIN      
        1 taaacagaaa ggaaaaccga taataatgga atggacgccc cgaccatgaa gcaacggtag
       61 gatattactg ataaaccttc cagtggaggt gcgttatggc cgttttaacg attagcattg
      121 acctgacaaa aaatgaattc caaatccacg gtctgggtag gaacagaaaa atctaagctc
      181 agaaaacgga ttaaagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag
      241 aactacgttc catggcagga gttcgtcaac acggttggag ggccattccg gcagtatgct
      301 cagaatatgg cgcagatacg cttccggatc gataccgttc agacggcagg tgcctgcttt
      361 tctggcaaca agcaggaatc tcagcgctga agcaggaatt agaggtaaaa acgccctacc
      421 gggccatgaa tcacccggtc attggagtag taaccaaagc agatttagcc agcatggaac
      481 aaatatcttt ggtgaaaagt tggttacggg aagctggagc gcacaacgtg ttagtaacca
      541 gtgcagttaa taacaatgga gttacagaac tttttgcctt gctgcataca gaagaaggct
      601 gttgttaatt tcgattggtt ctcggatgag aatgatgacg gtgatacaca gataagaatc
      661 aatgagcaat gagggttgcc gggcaaccct cattgaataa aacgggaatt acttcgcttc
      721 gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc gttctttgga
      781 gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct
      841 ggcgatgatg ccctggattg cttcaacgct catccccggc ttgatgtaat cgctcaggtt
      901 gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt cctgaatttc
      961 acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt cctggaatga
     1021 aggcttggta gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca
     1081 accgctattc gttagggcca acattactca tagctgccgc agatgacaat gcttttatcc
     1141 cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgaa
     1201 gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact caagctgatg
     1261 cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacaa ccttcaggtc
     1321 atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt tttccgtatt
     1381 ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg
     1441 ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg
     1501 gcgcgtggcc cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat
     1561 ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg cgggcgcaat
     1621 cagtccacgc attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg
     1681 gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact
     1741 tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac cggtaaacaa
     1801 cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg tatcaatttc
     1861 acctgtcggg gaacccacaa caattgccag cgtcacccca atcaataaac cttgatacgg
     1921 tttcttgccc agcgccagcc aaccgcaaag gaacatggcc gccgcgcacc agactaacat
     1981 cagcggtaac gagattaact ccagttgcag agcgataaga cctaaaatcg aacccaacac
     2041 cgtaccgcca atacgctcaa aggcgcgagg gacaacgtta ccccagaacg agattggccc
     2101 cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa acaggcggat
     2161 aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacaa tgcggtaatg
     2221 gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc agaaactccg
     2281 taatgagatt aaaagcgctc ataactcatc tgtcggccag aaggcgcgcc tctgaattat
     2341 ccgacggaca aaccgggaac agtgtaattt cccggcagga gcgaaaaaat tgaaaggcgc
     2401 atcttactct tttcgctttc aaaaaaaaga gtggtcatcg cgttaacaca ccgccctgag
     2461 atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca gtacccatct
     2521 tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc ataagcactg
     2581 tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc accgactaca
     2641 cgagctaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac gccctcactg
     2701 ttttcgggga gcgtaaagta acccggcacc gtcacgacgg tgtcgcagcg taatttttcg
     2761 gcgggtgttt catctggatt gtcgtaatag acagcaaccc attccttcgg cacaatattt
     2821 ttgctatcta cccacatcat caactgctca aaacctttct ttaccgtctg ttcccacggg
     2881 ccaacgagat ggaaacctgc aacggtacgt ttatcttcct gcttaatctc gtagttcatg
     2941 acgcctccat taatactgtt tttatataca gtatagttgc aatattaaaa ccacaaggaa
     3001 tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa agaaaggtca
     3061 ggccttatgg tggaaataat cactaaggcg agaaaacatg ctgccttccc cgacagattc
     3121 cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt caatttcccc
     3181 tacccgctga tgggcgctaa ttggcgcggt gagttcttta ccatcaaggg tatatttggc
     3241 tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt ccgttcccag
     3301 gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt tcccacggtg
     3361 caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc ttgcctcttc
     3421 ctcacgacct tttgcactgt cagcccccat tatcactgca atgagacgat gctgcccatc
     3481 tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt tcaggccgtc
     3541 aacattcatg gttttatccc acaataatcc gttacggttt tgctgggtga taccgttcca
     3601 ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga tgatagcgcg
     3661 agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca gaccatgcac
     3721 tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca tcatttcaac
     3781 aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt catttccgga
     3841 atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa
     3901 catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc gccctacggt
     3961 gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga catagcccgt
     4021 catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg cggtgaggat
     4081 ctgtccggtg gtgtaatcca tcagtaccca ggacccggca tgaatctctg gaggctgagg
     4141 tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga acaaagaagc
     4201 agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata gctgttcttt
     4261 ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac taccctctca
     4321 tttttatctg acatgatctg ttgccactag ctgccaaatt gtggcgctaa agctgattag
     4381 cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc taatgatgaa
     4441 tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg gcacgcaccc
     4501 cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat tcaatgtcat
     4561 cggcgaaccc gaagtctttt actgcaagcc cgcggatgac tatttgcccc agcctggagc
     4621 agtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa acgaagccgc
     4681 gtttgccgcc cgtattcact cgctttttaa cgtaccgaag acctgtattc tgggctacaa
     4741 caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt tctacgatcc
     4801 ttacgcctgg agctggcagc atgataactc gcgctgggat ttactggatg ttatgcgtgc
     4861 ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg gtctaccgag
     4921 ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg cccacgatgc
     4981 gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc gtcagccacg
     5041 cctgtttgaa tatctcttta cccatcgtaa taaacacaaa ctgatggcgt tgattgatgt
     5101 tccgcagatg aaacccctgg tgcacgtttc cggaatgttt ggggcatggc gcggcaatac
     5161 cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc aatgccgtaa ttatggtgga
     5221 tttggcagga gacatttcgc cattactgga actggatagc gacacattgc gcgagcgttt
     5281 atataccgca aaaaccgatc ttggcgataa cgccgccgtt ccggttaagc tggtgcatat
     5341 caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg ccgaccgact
     5401 gggaattaat cgtcagcatt gcctcaataa cctgaaaatt ctgcgtgaaa atccgcaagt
     5461 gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt cagataacgt
     5521 ggatgcacag ctttataacg gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt
     5581 gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg ataaacggat
     5641 tgaaaagctg ttgttcaatt atcgggcgcg caacttcccg gggacgctgg attatgccga
     5701 gcagcaacgc tggctggagc accgccgcca ggtcttcacg ccagagtttt tacagggtta
     5761 tgcagatgaa ttgcagatgc tggcacaaca atatgccgat gacaaagaga aagtggcgct
     5821 gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct ccgccgctgg
     5881 tacggcggag gacaatgttg agtcaggctt tttgaacggt gatgctccac gctgcatcgc
     5941 caatctgctg ataatcggtg atggcatgtc cttcttctgc cgcccactgt gggattgctt
     6001 cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc atttctgcca
     6061 gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca tccagctttt
     6121 taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg cgccttacgc
     6181 tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat aaatacaagg
     6241 ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc gatagagcaa
     6301 ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct acgtaatgtt
     6361 gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc aataaatgac
     6421 cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac gtcacccgcg
     6481 accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa catgcgtccg
     6541 gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac agcagtgaca
     6601 aagggatgcc agcgtttttc aaacagaata tgagcgatcc cggttcggcg cggcggtaag
     6661 gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag cgttatcacc
     6721 agcaaaacgg caaccaacgg ccacacagac aaattaaaag tctcagcaat agagttatgt
     6781 tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga acgcatcacc
     6841 gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc ctcacccgcg
     6901 cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc aaagatatac
     6961 ccacctataa cagtaccaag ccacgggaac gccccggctt cgtaagtcag taggcccgcc
     7021 tgaattaacg caaaaacccc cacactttgt accgaaatta caatcagcaa ggcgtaaaac
     7081 atgcgattat ttttcgcgag atacatatcg cgaaaaccac ctgtcaggca aaaacgccca
     7141 cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat cattgaaaac
     7201 ataaattaat taaccagatg aatgttaata gggaaattat tcatgactgg tggaatgcag
     7261 accaataacc aaattctcta aattagaaca aatggttatt aatgaggatg tcgataaaaa
     7321 agaataaaaa aaccggagcc aaatattcat tcgactccgg tttattatta caaaagatga
     7381 ttacgctacg tcttcgtact gcggaaccgg gttacggaaa ctcttggtga cgcaagccag
     7441 gtaaatcaga ccgatagccg cccagatcag acccagaacc attgagcttt cttccaggtt
     7501 aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg
     7561 atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga ccgacaggtt
     7621 aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gccgtagcca ttaccaggtc
     7681 gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg
     7741 ccatttcggg tgtacataac cgaagaagct tttcgggaat acgccgtcac gacccattac
     7801 gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt
     7861 ggagaagatc agcgcgccaa cctggaacgc tttacccgca acatacagca tgatttcagg
     7921 ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta
     7981 agttgcaaag atgaagatca tgccgccaat cagcgcggtc aggaaaatcg cacgcgggat
     8041 cacgcgctct gcatctttgg tttcttccga cagattgctg atgccgtcaa agccggtaaa
     8101 ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca
     8161 gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac
     8221 catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgaagtt
     8281 cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac
     8341 aaacatccac gatgggatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat
     8401 gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac
     8461 agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagccgaag gatagcggcg
     8521 aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc
     8581 tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa acagcgtcat
     8641 cggctgcata taggcaagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa
     8701 ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc tttacggtga
     8761 taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg
     8821 tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt
     8881 ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt ttagtcattt tttgacagcg
     8941 gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt
     9001 tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta
     9061 tttgctaaaa tttcatcccg ccaccagccg ctggcaagcc ctgcggtctg ttcattccag
     9121 ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca
     9181 ctatcgagaa accgttgcgc cagataacgg ggtaaatagc cgcagccgag gccgctaatt
     9241 tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt
     9301 tgtgaggccg tagaagcgcc tgcctgcgca gtatccccca ccacaatcgc ccgatatcgc
     9361 ttaataatac gacggtttaa cggctcttct tccagggcta acggatgatg aggcgcgacc
     9421 gcaaagactt gttccagatc gcccaaccgt gaaaaaccaa actcactgga agatggtggc
     9481 tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagggcatc ccaggaacca
     9541 ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca
     9601 atcagtggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt
     9661 tcccagcctt catgcagttt gatcgcctgc ttttccagtt cccgcacggt atgcagaact
     9721 tcccgccctt tctctaataa catttttccg gtacgggtga atttagcgcg gtgaccgcta
     9781 cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtgta actcagagcg
     9841 gacggcgtct tgtagagttt ggccgacgcc gccgcaaaac tgccttcttt ttctaaggca
     9901 tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg
     9961 cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg atactggtat
    10021 tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc
    10081 agactatttc tgaactgcgg aggctccagc cccagtaaac gggccatctg cggatagaaa
    10141 acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc
    10201 tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt
    10261 tcaccatcag gcgcggtttt tccggcaaag aagcgtccgg gatgacgtcc cggtccgacc
    10321 agccccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg
    10381 agttcttcta atactcgtcc actgttggtt accggattac gcggggtagt ttctttcacc
    10441 gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataatgcg gggaatacga
    10501 tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg
    10561 ccgctacgac gtgccggaag cgtaatgacc agcgcatcga catccatcag ggcatccaga
    10621 tcgtcagaat cgcaaactag ctcaggctcc atgcgaagca gatagctatc aatgccactc
    10681 attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc
    10741 gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat tgcgaccttt
    10801 ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact
    10861 gacaattcat tctatgaatg aaatcgaaac tgttagcaaa tataccattg cctgatgcgc
    10921 tccgcttatc agtcctacaa aacatcaata tattgaattt aggcaacatt tgtagacctg
    10981 ataaggcatt cacgccgcat ccggcaggaa caaagcccac tttagttaca gaacacaatg
    11041 ttcttgaggg ttttaaagac atattttcac gattactttt ctaacgtagt cactccaggc
    11101 cccctccttt ccccgctcat tcattaaaca aatccatcgc cataaaatat ataaaaaagc
    11161 ccttgctttc taacatgaaa gtggtttagg ttaaaagaca tcagttgaat aaacattcac
    11221 agagactttt atgacacgcg ttcaatttaa acaccaccat catcaccatc atcctgacta
    11281 gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca tgaacgcatg
    11341 agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcgattg ataacggttc
    11401 agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc atagctatgc
    11461 agaaatccgg ccgtttaagt gatgactcac gtgaattgct ggcgcgctgt ggcattaaaa
    11521 tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt gatattctgc
    11581 gcgtgcgtga cgacgacatt cccggtctgg taatggacgg cgtggttgac ctcgggatta
    11641 tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt gaagatccac
    11701 gctactttac cctgcgtcgt ctggatttcg gcggctgccg tctttcgctg gcaacgccgg
    11761 ttgatgaagc ctgggatggc ccgctctcct taaacggtaa acgtatcgcc acctcttatc
    11821 ctcacctgct caagcgttat ctcgaccaga aaggcatctc ttttaaatcc tgcttactga
    11881 acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga tgcgatttgc gatctggttt
    11941 ccaccggtgc cacgctggaa gcaaacggcc tgcgcgaagt cgaagttatc taccgctcga
    12001 aagcctgcct gatccagcgc gatggcgaaa tggaagaatc caaacagcaa ctgatcgaca
    12061 aactgctgac ccgtattcag ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc
    12121 acgcaccgac cgaacgtctg gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa
    12181 ccattctgcc gctggcgggt gaccaacaac gcgtagcgat gcacatggtc agtagcgaaa
    12241 ccctgttctg ggaaacgatg gaaaaactga aagcgctggg tgccagttca atcctggtcc
    12301 tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca ttgactggaa
    12361 tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgatct ccgcttctga
    12421 aagcattacc cgcactgtta acgatattct cgataacgtg aaagcacgcg gcgatgaggc
    12481 cctgcgggaa tacagcgcga agtttgataa aaccacggtt accgcactga aggtgtctgc
    12541 tgaggaaatt gccgccgcta gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt
    12601 ggcagtaaag aatattgaaa ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga
    12661 aacgcagcca ggcgtgcgtt gccagcaggt cacgcgcccg gtcgcttcag ttgggttgta
    12721 tattcctggc ggctccgccc cgctcttctc aacggtatta atgctggcga ctccggcgcg
    12781 tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg atgagatcct
    12841 ttatgcggcg cagctgtgcg gtgtgcagga cgtgtttaac gtcggcggcg cacaggccat
    12901 tgccgcactg gcgtttggta cggaatctgt gccgaaagtg gacaaaatct tcgggccggg
    12961 caacgccttt gtcaccgaag cgaaacgtca ggtgagccag cgtctggacg gtgcggcgat
    13021 cgatatgccc gcaggcccgt cggaagtgtt ggtcattgcc gacagtggcg caacgccgga
    13081 tttcgtggct tctgatttgc tttctcaggc tgaacacggc ccggactcac aggtgatttt
    13141 actgacgccc gctgctgata tggcgcgtcg cgttgccgaa gctgtcgaac gccaactggc
    13201 agaactgccg cgtgccgaaa ccgcccgtca ggcactgagc gccagccgcc tgatcgtgac
    13261 taaagattta gcgcagtgcg tagagatctc caaccagtac ggcccggagc acctgatcat
    13321 ccagacccgc aacgcccgcg atatggtcga tggcatcacc agcgccggtt cggtatttct
    13381 tggtgactgg tcaccggaat cggcaggtga ttacgcctcc ggcaccaacc acgttctgcc
    13441 gacttacggt tacaccgcca cctgttccag cctcgggctg gcggatttcc agaagcggat
    13501 gaccgtgcag gaactgtcga aagtaggttt ctccgcgctg gcttcgacca ttgaaacact
    13561 ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt actttgcgtg ttaacgccct
    13621 taaggagcaa gcatgagcac cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac
    13681 ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgacgtctg gctgaacgcc
    13741 aatgaatacc ccactgccgt ggagtttcag cttactcagc aaacgctcaa ccgctacccg
    13801 gaatgccagc cgaaagcggt gattgaaaat tacgcgcaat atgcaggcgt aaaaccggag
    13861 caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tgctgattcg cgctttttgc
    13921 gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta cagcgtcagc
    13981 gccgaaacga ttggcgtcga gtgccgcaca gtgccgacgc tggacaactg gcaactggac
    14041 ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg cagccccaac
    14101 aacccgaccg ggcaactgat caacccgcag gattttcgca ccctgctgga gttaacgcgc
    14161 ggtaaggcga ttgtggttgc cgatgaagcc tatatagagt tttgtccaca ggcatcgctg
    14221 gctggctggc tggcggaata tccgcacctg gctattttgc gcacactgtc gaaagctttt
    14281 gctctggcgg ggcttcgttg cggatttacg ctggcaaacg aagaagtcat caacctgctg
    14341 atgaaagtga tcgcccccta cccgctctcg acgccggttg ccgacattgc ggcccaggcg
    14401 ttaagcccgc aggggatcgt cgccatgcgt gaacgggtgg cgcaaattat tgcagaacgc
    14461 gaatatctga ttgccgcatt gaaagagatc ccctgcgtgg agcaggtttt tgactctgaa
    14521 accaactaca ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc tttgtgggat
    14581 cagggcatta tcttacgtga tcagaataaa caactctctt taagcggctg cctgcgaatt
    14641 accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt
    14701 tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc gaaccgccga
    14761 gtgattttca ggtggaccgt tttgacaaac tcgcctttga accgggcgtg atcccacagc
    14821 tgctgaagct gcaaaaagcg ggctacaagc tggtgatgat cactaatcag gatggtctgg
    14881 gaacacaaag tttcccgcag gcggatttcg atggcccgca caacctgatg atgcagatct
    14941 tcacctcgca aggcgtgcag tttgatgaag tgctgatttg tccgcacctg cccgcagatg
    15001 agtgcgactg ccgtaaaccg aaagtaaaac tggtggagcg ttatctcgct gagcaagcga
    15061 tggatcgtgc cgatagttat gtgattggcg atcgcgcgac cgacattcaa ctggcggaaa
    15121 acatgggcat taatggttta cgctacgacc gcgaaaccct gaactggccg atgattggcg
    15181 agcaactcac taaacgagac cgttacgccc atgtagtgcg caacaccaaa gagacgcaaa
    15241 ttgacgtcca ggtgtggctg gatcgcgaag gtggcagcaa gattaacacc ggcgttggct
    15301 tctttgatca tatgctggat cagatcgcca cccacggcgg tttccgtatg gaaatcaacg
    15361 tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc ctggcgctgg
    15421 gcgaagcgtt aaaaatcgcc cttggcgaca aacgcggtat ttgccgcttt ggttttgtac
    15481 tgccgatgga cgaatgcctt gcccgctgcg cgctggatat ctctggtcgt ccgcacctgg
    15541 aatataaagc tgaatttacc taccagcgcg tgggcgatct cagcaccgag atgatcgagc
    15601 acttcttccg atcgctctca tacaccatgg gcgtgacgct acacctgaaa accaaaggta
    15661 aaaacgatca ccaccgtgta gagagcctgt tcaaagcttt tggtcgcacc ctgcgccagg
    15721 ccatccgcgt ggaaggcgat accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt
    15781 gatccttgat actggctgcg ccaacctgaa ctcggtgaag tctgccattg cgcgccacgg
    15841 ttatgaaccc aaagtcagcc gtgacccgga cgtagtgttg ctggccgata aactattttt
    15901 acccggcgta ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgcg aactgtttga
    15961 tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc aactgctggg
    16021 gcggcgcagc gaagagagca acggcgtcga tttgctgggc atcatcgacg aagacgtgcc
    16081 gaaaatgacc gactttggtc tgcccctgcc gcatatgggc tggaaccgcg tttacccgca
    16141 ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact ttgttcacag
    16201 ctacgcaatg ccggtcaatc cgtggaccat cgcccagtgt aattacggcg aaccgttcac
    16261 cgcggcggta caaaaagata acttctacgg cgtgcagttt cacccggagc gttctggtgc
    16321 cgctggcgct aagctgctga aaaacttcct ggagatgtga tgattattcc ggcattagat
    16381 ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt acggcaaaca gcgcgattac
    16441 ggtaacgacc cgctgccgcg cttacaggat tacgccgcgc agggtgccga agtgttgcat
    16501 ctggtggatc tgaccggggc aaaagatccg gctaaacgtc aaatcccgct gattaaaacc
    16561 ctggtggcgg gcgttaacgt tccggtgcag gttggcggcg gcgtgcgtag cgaagaagac
    16621 gtggcggcgt tactggaagc gggcgttgcg cgcgtggtgg tcggctccac cgcggtgaaa
    16681 tcaccagaaa tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg
    16741 ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg cagtcagcgg ctggcaagag
    16801 aattcgggcg tttcactgga acaactggtg gaaacctatc tgcccgtcgg cctgaaacat
    16861 gtactgtgta ccgatatctc gcgcgacggc acgctggcag gttcgaacgt ctctttatat
    16921 gaagaagtgt gcgccagata tccgcaggtg gcatttcagt catccggcgg cattggcgac
    16981 attaacgatg ttgctgccct gcgtggcact ggcgtgcgcg gcgtaatagt tggtcgggca
    17041 ttactggaag gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc
    17101 catgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa
    17161 tcattggcga tatcgtgccg ctggcaaaac gctacgctga agaaggtgca gatgaactgg
    17221 tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagc tgggtatctc
    17281 gcgtggcgga agtcatcgac attccgtttt gtgtggcggg tgggattaag tctctggaag
    17341 atgccgcgaa aattctttcc tttggcgcgg ataaaatttc catcaactcg cctgcgctgg
    17401 cggacccgac gttaattact cgcctggcgg atcgctttgg cgtgcagtgt attgtggtcg
    17461 gtattgatac ctggtacgac gctgaaaccg gtaaatatca tgtgaatcaa tataccggcg
    17521 atgaaagccg cacccgcgtc actcagtggg aaacgctcga ctgggtacag gaagtgcaaa
    17581 aacgcggtgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt
    17641 acgacctcga acaactgaaa aaagtgcgtg aagtttgcca cgtcccgctg attgcctccg
    17701 gtggcgcggg caccatggaa cacttcctcg aagccttccg cgatgccgac gttgacggcg
    17761 cgctggcggc ttccgtattc cacaaacaaa taatcaatat tggtgaatta aaagcgtacc
    17821 tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc gcgaactgga
    17881 ctgggaaaaa accgacggac tgatgccggt gattgtgcaa cacgcggtat ccggtgaagt
    17941 gttaatgctg ggctatatga acccggaagc cttagacaaa accatcgaaa gcggcaaagt
    18001 caccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt
    18061 cctcaacgta gtaaatattg cgccggactg cgacaacgac acgttactgt tgctggcgaa
    18121 tcccatcggc ccgacctgcc acaaaggcac cagcagctgc ttcggcgaca ccgctcacca
    18181 gtggctgttc ctttatcaac tggaacaact gctcgccgaa cgcaaatctg ccgacccgga
    18241 aacctcctac accgccaaac tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg
    18301 cgaagaaggc gtggaaaccg cactggccgc aacggtaaat gaccgctttg agctgaagaa
    18361 tgaggcatct gacttggtgt atcacctgct ggtgctgttg caggatcagg ggctggatct
    18421 cggagaagtg attgataatc tgaagaatcg gcattaacgc gttggccccc gcagcaacgg
    18481 cgggggtagc atctctcagg atttgctttc cagcaatgta gtgtagagat tgacataggc
    18541 gtcaatcata tggtcggcag taaacatctc ttcaaaacga cgacgcgagt tttcgccata
    18601 gcggttggag gtttcctcgt tattccagag ctcattcatc gcctccacca gcgcctggct
    18661 atcattcggc ggaatcacgc aaccactcac tttgtcctgg ttaatgaaag aggtacctgt
    18721 accgatctcg caagagatca gcggccttgc aaagcgtgcg ccttccaata acgtaatgcc
    18781 aaacgcctca gagcgcagat gcgaggggaa taccacgccc cggcagagct ggaagagaat
    18841 gtacttatct tcgtcgttga gcatgccggt aaacatcaca ttgctcagcc cgcgctgctg
    18901 cgcttcacgc cgcacttccg attccagcgg cccgccccct acaaccacca ctggcagtcg
    18961 gctacgctca gcggcatcca tcagaatatg cagccctttg tagtagcgga aagtgccgac
    19021 gaagagaaag aacttatcgc cgacagtttc ccgccagtgc gcgaccctct gcggatcgtg
    19081 ctgcacgtcc tgctgctcca gaccaaacgg gatcaccacc gttttatcca gatatttttt
    19141 cagggtctgg ctggaagcca cgtaattggg cgacgaggca acgatgcaat ctacgccgct
    19201 gagaaatcgc tcctgcagcg gctggtacag cttcattaac cgtttttgtt tcactatatc
    19261 agagtgatag gtcaccacag tcctggcgtc aggccgcgcc gaaagatgca gcatatccat
    19321 aaagggaaac ggaaaatggt agttgatgat atcgtagtca tctttaatca gcttaaaacg
    19381 ctttaacgct ttcagcgaaa acggcgtgga ggcaatttca aaaagctgtt tattataaat
    19441 gacccgatga tcttcgtaag cgacaggacc tgtatctttg tccgggctaa aagtgaaaac
    19501 atcggctgcg attccccggc gggcgcagcc ctgacttagc tgataaatga cctgctcaat
    19561 accgccgtag gtatcgggat agtaagtctt atagacgtgt agaactctca actgacaccc
    19621 ttagagtaat ttataggcgt taatggtctg tgtcgcacag ttctcccacg aaaactgttt
    19681 cgcctgagca agaccgcgcg cggtggcgat ttcccgccag ctatcatctt gcaggctctg
    19741 caaaatttgc gcgctaatcg cgtctatatc attaggatcg gcaacgaggc cggcatcgcc
    19801 aacaacctca ggcaaagagg tgacattgga gcatactacc ggcacaccgc aagacatcgc
    19861 ttcaagaata ggtaaaccga atccctcgta gaaggaggga taaacaaaga ctctggccgc
    19921 tgcgtacaga tacggcaggt cttcatccgg aacatatccg aggtaacgga tccagccttc
    19981 ccgagtaccg cgctcgacta actgccacag cacatcgtct tcccagccgc gatagccgct
    20041 aaggattagc ggatagcgca tgcggatctc catcggtagc agctgatagg catgcagcag
    20101 gcctcggata tttttacgtg gctccatagt gccgatatat agcgcgtagg cctgccacgc
    20161 cagctgatat ttctgcagta ccggcagaca ctctgccggg ctgcgtggga tatagtcact
    20221 gctgcaggct agcttggtgg ttacgatccg ctccgccgga tagttgaaca agcggataat
    20281 ttcactgcgc gagaaatcag aaacggtcag gatcagcttt gccgaatcca gactctcatg
    20341 cagggacttc tccatatagc gaacccgatc ttttggatga tattccgggc aggtaaaaat
    20401 ggatatgtca tgaaacgtgg tcacggcgcg ttccagttta tgcggcagat aaaaattggg
    20461 gccatggtag atataatcct tatagtcgcg caatgcccac gcctggcgcc gcggatgcag
    20521 caagcgataa gcctcaatca acagcgtctg tcggcgtaga aacgccgaca gacgaccatg
    20581 attgctggct ttggtatcgc ttttattctc caccaaaggg atctgttcta taaacgacgc
    20641 accgtgaaat agctttaatt cttcaatttc gcgggcgacc gccagccgct taaccagctc
    20701 cagggaatac cgaccgatgc ccgttaatgg gtatttaatt ggctcagtag caaaaataat
    20761 tttcataata aatgacacat tatctaaccc ctcttgtatt caaaggcaaa aaatacaaga
    20821 gagatatttt tatttaaaat atttagcttt agctaagcct tgacacatat taaccagacg
    20881 gcgtgaaaca gcttgataat catgttgttc ccgccatgca tcggcacgtt gttgagtcct
    20941 ggttgcccaa gagttctgtt cacggatgga attcaggatc cggtcaatcc cctgactgat
    21001 atcatcgacg ctgcatccat caaatttaaa gacggcatcg tccaaatcat caaatatcgc
    21061 caggggcgtt accgcaacag gtttttgagt cgccatacca taacgtaccg ccccgctagc
    21121 agactcccca gtattctgat aagcaaaaat cagaagatcg gcttctgaaa gcaaccgcag
    21181 actctccgca tcaggtagga aatcattatg catctcaatc agatcggtaa cacctaaccg
    21241 ctgagcagca gctttaagct ctgccaccag atcgcgtgac tccccaacag gatactctgc
    21301 gttcaccagt cgtaaacgaa ccggtttacc ggcttgcttg agtctatgga cggattctac
    21361 tagttccatc aggcccttat gcggtaagca gaagccatag ctcgcaatta gcggtaaaga
    21421 ctgctgttga cgtgtgacgc tcgctgcgga gtagttgata acaccgtgcg ggaataaagc
    21481 aacattcgca gttaagccta aatctttaag gcggttcata tcggcaatcg aatgcaccaa
    21541 caaccggtcg caaagtgcca gcgcctcttt catttcagca agacggaaat tccagctcgg
    21601 ctctttttcc agcggatcca cagttgagtg catcgtcata acaactgaac gaccggcgtc
    21661 atgctggcga cgaataaacg ccgacagttc tcgatgatta aagaatccat agttgaattg
    21721 gatcacaatg acatcgagtc tcagagcatc aatgtgtggc tggaggtttt ccagatagtt
    21781 gctctcttta cctacaatcc agttgcgaag tacaaactct tcgtctgcac acacaagatc
    21841 gccagcgctg acctggggag caaaaacaac atccgcgcca tgaggtgcgc tttccaccag
    21901 atgctgggaa taggttgcga tcccacattt cgtgttccag gtcgtcaccc agccaatgcg
    21961 cgcccgatgc tgtgcaatat caatatgcgc acgcagagtc ttgaccgcct ggcaagaacg
    22021 atcagccacc gctttccagg taaactgctg cagaagaagc tcgcgaccag cattggccat
    22081 gtcacgcagc actgatttat cggttgaggc tgccgctttt aatgcatctg ttaagttgtc
    22141 aatatccaca ctggcccagg ctgaggaaaa cagaccaaag tgcgttttta cccgagtgaa
    22201 ctgatagtca accagccatg aattttgtga attaacaaaa tcaagttgcc cgctccaatt
    22261 ggtgacgata gccggtagcc cactcagcat tgcttcagca ataggtaaac caaagccttc
    22321 agcgcaacct ggagcaacca aaacatcaca gctttcataa agccctttaa gctcggtggc
    22381 tgacatatcc tctttgatca cttcaacttt gggatagtct atgaattgag cctgggcctg
    22441 agccagccat gcgtcaattt cattgtgcgg attgttaaaa gtcttaatga ttaagataac
    22501 attgtcacga cgagtgaacg ccttccccca agcctgaagc attgcctgta tccctttgcg
    22561 tgggaaacaa gaagagacgt gcaggaaacg gaatgttccg tgatccacat cttttgtcgt
    22621 ctcggctggg atattgagcc aatggtcaca gccattgcca acaacaaatg cgggcacatt
    22681 cagtccgtta tcaatcagta ttttacgaac atgttccgaa gtacacagca ctccgtcaag
    22741 ttcccgatta aattcattga tccacggttg cggaaagccc gtttcttccc aagcataaca
    22801 atgaaggaat tttactttgg catccatttt ggtaacccgc ggtggataga tatttctgct
    22861 aataatatct atcttttcgt tactcttgcg ggtctgacat tgattataaa atgccagaag
    22921 atcactattt tccgactgtg ccataaacga ggcatctggg gcaaaatcac ctggtccttc
    22981 agtggaatgc aataaaacct ctacaccatc ggctgagagt gcccgggcaa attcgcggtt
    23041 gaccaacgcc agactatatg agctatcgaa tgggccttcc acacgccagt tcagcgattt
    23101 atcgtcgatt tgataaagtt ctgccgtttt cagattgtaa tcaatggccg ttgcgcacaa
    23161 gcgcagatcg cggtcatctg gctgcccttg tgagatagca aggatcttct gaatcaaagc
    23221 ttcaggcaaa acctgcgcag taccggtgtc ttctaccgcg atcttttcga aagcttccag
    23281 agcagtcacc gcagctttat cccaagagaa attacgcgct tgctgctgcg ccatttcttt
    23341 cagacgcgcg aggaaggtat catcagtcaa acattgcgcg atcttatccc tcatggaaga
    23401 gacagaatac gggtcgaata atgcctcagg attaccgatg acttcaggaa tactggtgac
    23461 atttgagcca atcaccggcg caccgcacga cattgcttcc agaaccggca acccaaaacc
    23521 ttcatgtagt gaagcaaaga tgaacagctt acataggcga tagagctgga tcagctcatc
    23581 ttccggcaca taaccagtga gtaccagttc gccctgctgt aaaccattac ctgacgcaag
    23641 ggattccaga tactgacgat caccgatgga aagcttactg acgatgacca gttgatgact
    23701 gcgacgtaag gcatcactga gcccggcata ggcctcaatc aaccgtttaa agtttttcct
    23761 tgagtcaaat cctccgggcg catacaacat gaactcgcgc tgaataccgt aatggccaag
    23821 gacggtctct ttttcgctcg ctgccacctc cgccatacaa aactgagaat cgactgcggc
    23881 tgcaatattc tgcacatgat cgccctgtaa atgcagatgc tcgatcgctt cctgtgcggt
    23941 ataagcagag ttagtcaaca aaaggtcagc gttttttaac cactcaactt tctgtaaata
    24001 gtagggtttg tatacatcgt cctgcagata ggtttccgcc tgcacgaggg ggataagatc
    24061 gtggtgcagt acggctactt tatactgacg actaaatttg tgtaccgatg tagccgcatc
    24121 gtcgacatga ccttcaaaca aactcgtaat gaaaacgaca tctggacaca atgattcaag
    24181 aaacgcttcc cgaatcagtt ctgcgctctc ccgacgccat tcattacctt ggtccatcgc
    24241 acgaacaggg cctacggcat gccatacgac tatattttct gccggcagga gatcggcaaa
    24301 ttgcgcctta atatttgcaa tcgactcatc cagcatggcg gatagcgcga tgaaaatctc
    24361 atgccggctg ttatttctga ttatgcctct ggcgattgcg atactataac gaccgatgcc
    24421 acgaaagcgg ctttccgtct gtgcgccttg taaatctatg acaatacgca tcgttatttc
    24481 tccgtatttt tattttttaa ttcgttataa atactcatcg cgcgacttgt catttcttca
    24541 gtacccacat catatcttct ggactgcatc atcatcgtgt cagattgcac cagcattacg
    24601 cgttggaaca aacgttgtag caatgtatag cagccagttt ttctcagaac cttaaacaga
    24661 taaaccttag acttagggta acgatggaaa aagaccagcg agagcgccat ccctttgcgc
    24721 aatatttttt tcccagccct acgggctcgc actttagccc cttcctgacg taataattga
    24781 cgttgcaaag acaaccagcg tagtggttga gtaatacgcc atgaacgact gttatgtaaa
    24841 tccgccattt gctgatgaag ctgatggttc tgatcgatga catgactcag atcgtcaacc
    24901 tcaccttgca acgtctgaat agtctctgac atttggctta tctgttgcat ataggtttgg
    24961 ttcaacgtct ccagccgtga gacaacggac gaaaactgtt ggcgcaaaat cgcatcgtaa
    25021 cggttgctca gagcatccag cgtcagaccg tactgctggg taaacagggt gtcaaagcgt
    25081 tcaagaatat ctgttggcgc tgctttctga gcaatgatgc tgtagtcggg gctcacccct
    25141 ttgagtacat cgaccaaatt aacggctgca tccggagatt gaagaacctc tttttcctgc
    25201 agacgaacgg taattgctcg ggtaaaacca taatgaatag gtaaaaactc aagcagtggc
    25261 ggtggcagag gatgattatg cgttggatcc atataaaatg aacaggtgcc gacgcttaca
    25321 ttctccgggt tcggcgtttc gaggatcagc aaaccacctg gtttcagcac ccgtagggta
    25381 tgcatggcga gttgctgcag gacctcaaac ggcaaatgct cagcaatatg aaaaccggtc
    25441 aacgctatca ggctctgatc cgcctgactt tgcaaaaact gcagacaatc cattttctgc
    25501 acattcagtc cggcctccct ggcgcgcgcc agcatgccat catcgagatc gacgccgatg
    25561 ttcgcaatgc cattttcagt caggatctcc aaccattcgc cacgtccgca accaatatcc
    25621 gcaatcacgc catcaggata aatgtccttc agacctgcaa gaaaaggtaa ataaaaactc
    25681 aagcggcgct tgatttcttc aaccgaacct ctgtgtcgtt cttcaaatga acgataaaac
    25741 gacgaaccca ttaagaaatc tccagttcag gcggcaacca ggacacgccg acaaactctt
    25801 gttgttccgt gttaacgacg ttgaatacca cggccagatc gcgccattca tagtttttgc
    25861 cgaggtgcgt actggaagta tgcaacgcga cagcgacaga ataggagcca acccccaatc
    25921 tcgcatcgaa agaaaataag aacgaacgct tttctccttt tttcagggag gtgagtgtct
    25981 gattgagatg gtacgtattg gtcccgaaaa tcggctgccc aagtcgatcc ttaatcatat
    26041 atccgacaac aagctcagga atatcgtcct tgacctcaac gttgacctgc aagctgacac
    26101 gatgccctac cgaaacaaat tcagtcacat tgccctgttc atcgagaaga tgaacctcag
    26161 agatagtcac ctcaccagtg cctgaaacag tttgcgtttt accattatgc tcaacttgtt
    26221 taatggactg attttgttta tcggccagaa gagcattgta ataatccatc actgcttcag
    26281 gttcaccttc catttcaatt tggcctttat tcaataaaat ggcccggtcg caaatgcttt
    26341 ggatcgcttg tttatcatgg gataccagca acagcgtggt cccttcctga cgaaattttc
    26401 gaatacgctc aaagctttta tgctggaaat atgcatcccc aacagataat gcctcatcga
    26461 taattagcac atcaggacgg atagccgtcg ctacactaaa agctaatcga acttgcatcc
    26521 cactggagta gacgcgcaca ggttgatcga tatagtcccc aatctcagca aactcttcaa
    26581 tttgcggcat cagttcagtt attttctctg acgataaccc caacagttgc ccagacatat
    26641 aaacattctg ccgaccagtg aaatcagaat gaaaccccat ccccaattcg agtaatgcag
    26701 cgacacgtcc ggagatttca atttctccag tcgtcggcct ggacgtcccg gttatgagtt
    26761 taagcagggt actcttgcct gcaccgttga taccgataat accgacagcc tcgcccggag
    26821 cgacttcgaa attaatatcg cggaggatcc atttcaaatt atggcgtttg gtattcagag
    26881 gggataacca ttcgatcagt ctcccggtct ttgagtgata ctggcgatac gccttaccga
    26941 cattatttac tctgatataa ctcattataa ttcatccacc atatccgccg catgcttgcg
    27001 gaacatcctg aaaccgatga cgcagaaaat aatggcgaga gccaatactg gccatagcga
    27061 ataccagttg ggggccagat gataggcgaa gatggactga taagattgca tgatccgagt
    27121 catcgggtta tacatcatca gattttttgc ccatgcaggt aatgaattca gtacataaac
    27181 aatgggtgtg aaccaaaacc agaattgcag cgcaacgcca accagttgcc ccacatccct
    27241 gaaaaagacg ttcatgacac caaggatcat ccccagccca ccggcaaaca ggatctgcaa
    27301 aagcaggacc ggtatcaccg agagaaagag ccagccgggg aagttaccgg tgacaatgat
    27361 aaaaattaga aacagactga aaataatcgc gaaatttagc accgccgata acgtcacgat
    27421 gatcggcaga cagattttcg gaaaactgag tttcttgatc agattagcat tgttaataaa
    27481 tacgctctga cctttatcca gcatctcagt aaataatccc caggtcagta ccccggaaca
    27541 gagataaata ctataggcaa acgacccggt attatcgggc attcttgcct tcatcacctc
    27601 ggaaaaaacc agggtataga ccagaatcat agagagcggt tgtaaaacga gccatagtgc
    27661 gcccagcata ctagtttgat agcgtgcctg aaaatcacgt ttaacactgc tccagataaa
    27721 tccccgataa cgataaatcg tcgttagtaa atctctcacc ttattccccg cttaacaggt
    27781 gtaacagctc ggtcgttttt tcattcataa gcgccacatc agctctggac tcaacgttca
    27841 gacgcaccac cggctcggtg ttagacgtgc gcaggttaaa gcgccattca gggtattcaa
    27901 tactgatccc gtccgttgtg tcgatgtgtg cagccgccgg ctcatactgc gcccggatgc
    27961 gggcgatcgc ctcagcagca ttacttagct tgcggttgat ctctcccgac gcagggaacg
    28021 ccttctggcg gtccgccacc agcgatttca gcgagctgtt cttcagacac agcagctccg
    28081 ccaccagcag ccacgggatc atcccgctat cgcagtaggc gaaatcgcgg aagtaatggt
    28141 gcgcactcat ctccccgccg tagatagcgt cttcctgacg catccgctcc ttgatgaacg
    28201 catgccccgt cttcgacatc accggctggc cgccgttgcg ggtcacgatg tctaccgtgt
    28261 tccacgtcaa gcgcgggtcg tgaatgattt tcgctcccgg ctgcttctgc aggaacgcct
    28321 cagccagcag gccgacaatg taatacccct cgataaacga agcttcgtca tcgaacagga
    28381 agcagcgatc gaagtcgccg tcaaaggcaa tccccatgtc ggcctgatgc tcgcgcaccg
    28441 cgtcggcggt atcctggcgg cactccggca gcagcggatt cgggataccg ttagggaaat
    28501 ggccatccgg ctggtgatgc accttgataa aggttaccgg caccccagcc gccgcgaagc
    28561 gtttctccac ctcatcaatc acgtgccccg cagccccgtt tccggagttc accaccaact
    28621 tcagtggacg ggtgaagttc gagaagtcca cgtagctcat cagatggtca acatactcct
    28681 tcagtaccga tatcttgctc agggtcccgc gacgcgccgg gtccactggc gggaactggt
    28741 tttcctccgc caggcgctgg atatcccgca ggccggtgtc gccgctgatg ggcttcgcat
    28801 tctcgcgcac cagcttcatg ccgttgtagt tcataggatt gtggctcgcg gtcacctcga
    28861 tgccaccatc taccccaagg tggaaggtgg caaagtaaat ctcttcggta ccgctcaggc
    28921 cgatgtccag cacgtcggta ccggcgtcca ttaacccgcg ggccagcgcc agcttcagcg
    28981 actcgcttgt gaggcgcaca tcgcccccca ccactatctt cccgggtttc agaaattcgc
    29041 cgtaggcgcg accgatacgg taggcgatgt cctcgttcag ttcctcaccc agttcaccac
    29101 ggatgtcata agctttaaaa caagttaact gtgtcattct gcgtcttgtc ccgaaaataa
    29161 ttagcaacga ccatactggt ctttaatacg aataatgtcg tcctcgccaa ggtacgcccc
    29221 cgactggatc tccagcactt ccagcggaat acagccaggg ttttccaggc agtgctcggc
    29281 gccaatcgga ataaaggtgg actggttctc ggacaacagg aactgcttac cgttgacagt
    29341 cacctgaccg gtgccggcga gaataaccca atgctcggcg cgatggtggt gcatctgcat
    29401 cgagaaggca ccgcctggtt tcaccgtgat gcggttgacg ttgaagcgcg gggtctggac
    29461 cactacgtcg caacggcccc aggggcggta aatctcacgg tggcgcttgt actcgctgcg
    29521 ctggttctgc ttgaggaact cgaccgcctt cttcacgtcc tgggaacgct cgcgattcat
    29581 caccagcacg gcgtccttag tgctgacaat caccagattc tctacgccaa tggccgccac
    29641 tagcttctcg tcgctgttga tgtagcagtt ttcgctgttg tgtacccacg cgtcaccgct
    29701 gaggacattg ccctgctcgt ctttcgggct gacctcccat agtgcggacc aggagccgac
    29761 gtcgctccag tcagcatcga gaccgaccac caccgcatcg gcggttttct ccataacggc
    29821 atagtccacg gactcatccg ggcactcgca gaaaatatca tgcgggatat tgatgaaatc
    29881 gctgccatta tcggcggcat tcaccgcagc ctggcaggct tccaggatat ccggacggta
    29941 tttggccagc tcgatgaggt atttcttggc gcggaacata aacatgccgc tgttccagta
    30001 gtactccccg gaggcgaggt aagcctcggc gcgctcgcga tccggcttct ccacaaagcg
    30061 ggccacctgg tacgcggaat gggcactatc ggtgagcgcc acgccgcgct gaatgtaacc
    30121 gtagccagtt tccggggcat tcggcacgat accgaaggtg accagatgac cttcatcagc
    30181 atactgctcg gcgacccgga tggcgtcgtg gaaggccgac tggttattga tgatatggtc
    30241 agcggcgaga accagcatca gcgggtcgtc gccgtcgcgg gtggcctgaa gggctgccag
    30301 ggcgatggcc ggggcggtgt tgcgcccgac cggctcaaga ataatattat tcgacagctt
    30361 attgagctgg cgtagctgtt cagccaccag gaagcggtgc tcttcgttac agatgaccat
    30421 cggttcatgg atttcaaggc ccgagagtcg ggtgatggtt tcctgcagca tggagttctg
    30481 cccgaacagg cggaggaact gtttcggata aagctcgcgt gacatcggcc agagacgact
    30541 gccggtacca ccagccataa ttacaggaag taacataatg aaatccttaa ataaataatt
    30601 gttttatttt actatcaatc aatcgaccaa caggttgaat ggcctttttc aggtacgcta
    30661 ccgccattgg ctatcgcaac caaaatgcgt agtgctcccg tgctccaggc taaacaccac
    30721 cctctttcgc ttcagacggt cggcagatag ttcacgcagc ggtctgaaaa accatcctta
    30781 cggaacgggt atcctgaaaa cctaaaagca gagatatgcc ctgaggcatg tctctgcttt
    30841 taatatgtat ctgttcctca ccaggccacg aaacgcccca caatttccac atttgccgcc
    30901 agagtaacca tcaggacctt ctcatttacg cggggcaaaa cacatgctct caccgccatc
    30961 aaaaaaaacg aatcaagtga tttaccgatg ccagcaacaa tcagaaaggg gcatttacga
    31021 tacggggaaa gataagaaga gacatcgtat tcgccgcccg gcaggcaacg cgcgttcggg
    31081 cttaatagct ctttgcacac gacgacggcg ttatgttgcg actggtggga taaacgactc
    31141 atgcctctac actgcacatt ccacaaaaaa aatcttcgca gattatagcg ttggatgctg
    31201 ggaaaaaaaa tataaaagcc gactaattaa tggattactc aaataataca catcttaaac
    31261 atttaccaaa caaattaatg ctaatccatt gatataaaag taataataaa gagaatcatg
    31321 acattttttt gcctggttgc taaataatct gctgataaaa aatacagatt tgtacaaaat
    31381 tacgccatgc tcgctgtgag taaccccgtt ttgcgacggt acatactaac caatgaggtg
    31441 cctgatttga tgtatcacct gctggtgctg ttgcaggttc aaaatctgga tttaacaaca
    31501 gttatagtga atctgcgcac gcggcatcag tgagttgcgg ggtaggcgga tgtgatgttg
    31561 ctgtcgcttc cggcaacaac tcatacgtca ttgcccggtg tgcaggttta tcgtgtctac
    31621 aaattttctc aacatatgta agctggataa gatacgccgg catcgcatcc gacaaaaaaa
    31681 cgggcacggt gtgaccaccc tgcccgcttt cttttagctc aaaaaatcac ttcgcgttgt
    31741 aattacgcag cgcgttacgc cccagcacaa tacccgcgcc gaccatgccg cccagcagca
    31801 ccgccagaat caaggtgatt gccttcttag ggctatcacg acgaatgggt aacgtaggtt
    31861 tcatcacata gcggtaagcg tgaatatcaa gatcatcaac ctttaagctt tcgatatcca
    31921 gcaggttttg acgagtctga tagtaggttg acgagaacac caacggacgg ctcgcttcgt
    31981 gctttaccat cgactccagt gcatcactcc ccaaaaggaa catggtatcc tgggtcacgt
    32041 cctgtgtctg ctgaatctgc ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    32101 cctgaatctg acggatacgc agatctttct gctcttgcgc gactacttcc tgggtcttca
    32161 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcagatct ttttcaagtt
    32221 cttcatttac ctggtcgtca acttgctgga tatattgagc cagctgtttc tgcgcagctt
    32281 caggcgattg cccaacataa gacaccgcta aaggtaatga ctgattttta acagtaggtt
    32341 caatagtcag tttttcaggt tcttcctgat tatcaagcgt ttctgctaat gcagaaaatg
    32401 tcgtgctgaa gcgaccaatt aaaatcgact ggatctcaga cactttcggc gcggctgaac
    32461 cataaatcac atttatggca ttgttataac cagcgatttg ccccacatca ggttgagtaa
    32521 cgatagctgt ggaagtccat ttttctttgg caacgaccag atatcctatt gccagggcaa
    32581 tagcgacaat gacagaaatg atgattgtca tcttgtcacg ccatagttgc accagtaaat
    32641 caatcaaatc aat
//
LOCUS       DAFAVF010000038        32309 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFAVF010000038 DAFAVF010000000
VERSION     DAFAVF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.038"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(71..1831)
                     /locus_tag="LGK42_001764"
     CDS             complement(71..1831)
                     /locus_tag="LGK42_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBK2658021.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1961..2653
                     /locus_tag="LGK42_001765"
     CDS             1961..2653
                     /locus_tag="LGK42_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK2658022.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2852..3940
                     /gene="serC"
                     /locus_tag="LGK42_001766"
     CDS             2852..3940
                     /gene="serC"
                     /locus_tag="LGK42_001766"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBK2658023.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4011..5294
                     /gene="aroA"
                     /locus_tag="LGK42_001767"
     CDS             4011..5294
                     /gene="aroA"
                     /locus_tag="LGK42_001767"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK2658024.1"
                     /translation="MESLTLQPIASVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5463..6227
                     /gene="ycaL"
                     /locus_tag="LGK42_001768"
     CDS             5463..6227
                     /gene="ycaL"
                     /locus_tag="LGK42_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBK2658025.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6400..7083
                     /gene="cmk"
                     /locus_tag="LGK42_001769"
     CDS             6400..7083
                     /gene="cmk"
                     /locus_tag="LGK42_001769"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK2658026.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7194..8867
                     /gene="rpsA"
                     /locus_tag="LGK42_001770"
     CDS             7194..8867
                     /gene="rpsA"
                     /locus_tag="LGK42_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK2658027.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9027..9311
                     /gene="ihfB"
                     /locus_tag="LGK42_001771"
     CDS             9027..9311
                     /gene="ihfB"
                     /locus_tag="LGK42_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBK2658028.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9518..11782
                     /locus_tag="LGK42_001772"
     CDS             9518..11782
                     /locus_tag="LGK42_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBK2658029.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11819..13567
                     /gene="msbA"
                     /locus_tag="LGK42_001773"
     CDS             11819..13567
                     /gene="msbA"
                     /locus_tag="LGK42_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBK2658030.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13564..14550
                     /gene="lpxK"
                     /locus_tag="LGK42_001774"
     CDS             13564..14550
                     /gene="lpxK"
                     /locus_tag="LGK42_001774"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBK2658031.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14587..15819
                     /locus_tag="LGK42_001775"
     CDS             14587..15819
                     /locus_tag="LGK42_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBK2658032.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15871..16053
                     /gene="ycaR"
                     /locus_tag="LGK42_001776"
     CDS             15871..16053
                     /gene="ycaR"
                     /locus_tag="LGK42_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBK2658033.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16050..16796
                     /gene="kdsB"
                     /locus_tag="LGK42_001777"
     CDS             16050..16796
                     /gene="kdsB"
                     /locus_tag="LGK42_001777"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBK2658034.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16950..17843
                     /locus_tag="LGK42_001778"
     CDS             16950..17843
                     /locus_tag="LGK42_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBK2658035.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="LGK42_001779"
     CDS             complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="LGK42_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBK2658036.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18735..19520
                     /gene="cmoM"
                     /locus_tag="LGK42_001780"
     CDS             18735..19520
                     /gene="cmoM"
                     /locus_tag="LGK42_001780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBK2658037.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19517..20839
                     /gene="mukF"
                     /locus_tag="LGK42_001781"
     CDS             19517..20839
                     /gene="mukF"
                     /locus_tag="LGK42_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBK2658038.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20820..21524
                     /gene="mukE"
                     /locus_tag="LGK42_001782"
     CDS             20820..21524
                     /gene="mukE"
                     /locus_tag="LGK42_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBK2658039.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21524..25984
                     /gene="mukB"
                     /locus_tag="LGK42_001783"
     CDS             21524..25984
                     /gene="mukB"
                     /locus_tag="LGK42_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBK2658040.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26245..28092
                     /gene="ldtD"
                     /locus_tag="LGK42_001784"
     CDS             26245..28092
                     /gene="ldtD"
                     /locus_tag="LGK42_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2658041.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28273..28821
                     /locus_tag="LGK42_001785"
     CDS             28273..28821
                     /locus_tag="LGK42_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBK2658042.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28848..29495
                     /gene="gloC"
                     /locus_tag="LGK42_001786"
     CDS             28848..29495
                     /gene="gloC"
                     /locus_tag="LGK42_001786"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBK2658043.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="LGK42_001787"
     CDS             complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="LGK42_001787"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBK2658044.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31092..32180)
                     /gene="ompF"
                     /locus_tag="LGK42_001788"
     CDS             complement(31092..32180)
                     /gene="ompF"
                     /locus_tag="LGK42_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBK2658045.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7854 a         7855 c         8701 g         7899 t
ORIGIN      
        1 gcattaaaaa aacgccccgt aatataactc agactaatca ttaagcctac attgcgtagg
       61 ctatttgatt ttattttgcc cagaatgctg ctttggcgcg ctgcagcttt tcgtaggcct
      121 tcaacaacga ctgatgcgca gcaaacgcgt gcagatcgct atctaccggt tgcaggccgt
      181 aaaacgccgc ttcgccgctc attgccgcac tggcggcttc tacggcatct gcgccgtaca
      241 tgcgaacaaa cgcattcaga tattgcagcg gctggcgatc ttcttcctgt gccagtaata
      301 acaacgtttg caggcagcga taatagttgg cgcgttccgg actaaatact gatgagttaa
      361 actccatcgt ccattcggtc cagaccagag cctgttccag atcgccacca gccagcgcca
      421 gcatggcttt taattcaccg atacgcaggg tgtaccaacc gttatccgac ccggtcgcca
      481 gacccaacag ctcacgcacg cgggtaaagt catcaaaacc ttcttcatcc agttgctcga
      541 tgaggttcag gtaatcttct ttttcccact cgctgcctgg tagcgaaaga atcgtttcac
      601 gtaaatggct gcccatactg ttattcgcga gccacagatc ttcagccgga taaatatcgg
      661 acatgccagg cacgataata cggcaagcat aaacgcccag atgctcgtaa tcggcaatat
      721 aaacttcttt atcttctttg ttgaagatag ccatcagtgt ggcgaactct tcttccgtgg
      781 tgccggagaa attccagtcc acaaacggat aatcggcatc ctgcttgaac aggtcccagg
      841 agattaaacc gctggaatcg ataaagtgcg tttcgaggtt ggtatgttca gcgacttctt
      901 catcatcgaa ggttggcgga gtaaacacat ccaaatcttt caggccacga ccttgcagca
      961 gctcggtcac ggtacgttcc agtgctacgc caaaatcagg atgcgcaccg aaagaggcaa
     1021 agcaggtacc gttagcagga ttgaacagta ccacgcaaat caccggatac tggccgccaa
     1081 gcgaaccgtc ataagcgaag attgggaaac cctccgcttc cagcgtttcg atggcttcaa
     1141 ctactgctgg gtaacgcgcc agcacgtctg ccgggatctc tggcaggctg atgctttcag
     1201 caataatgcg gtttttcacg tagcgttcga aaacttcaga caatccctga acgcgtgctt
     1261 cgttgcgggt attacctgcg gacataccgt tggagacgta caggttacca atgatattca
     1321 tcggaatata aacggtctga ttgtcggact gacgcgtaaa cggcaggccg caaataccac
     1381 gatcttcgtt accggattgt aggtcaatca gcatgctgcc ggtcagttca ttctccggat
     1441 cataaaacgc gcgcagacgg tcatcgagta gtccttctgg cacatcgtca ttttcggtca
     1501 gtgggaacca tttttcgttg ggataatgca cgaacggacc gttggcgatg gtttcgccca
     1561 gccagaaatc agcaaaaaag tagttggttg agagacgctc gaaatattca ccaagtgcag
     1621 aagccagcgc cgcttttttg gttgcgcctt taccgttggt aaaacacagt gcgcactctt
     1681 tgtcgcgaat atgtacagac cagacgttag gcacaggatt cagccaggag gcctcttcaa
     1741 tctgaaagcc gaggtctgaa agtttttgct ggaagcgagc gatggaatct tccagagcgg
     1801 catctttgcc ggggataaat gtttgcgtca tgaaaatcac tttagtcgta cggaaagcgc
     1861 gcaataatat gggttttatc tcaaaggcgc tatcaccgcc gccatgccga tgaactgttg
     1921 actatgcttt tagcggataa accacgataa gagcataaaa atgaaggcgt tcgatctcca
     1981 ccgtatggca tttgataaag tgccttttga tttccttggc gaagttgcac tacgtagtct
     2041 ttataccttt gtactggttt ttctgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     2101 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     2161 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     2221 ataccgcctg gtaatgtggt tgatggcgca cagtgaaaaa ctggaagatt tactggaagg
     2281 taagccggtt gttattattg aagatggcga gctggcctgg tcgaaactca ataactccaa
     2341 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     2401 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     2461 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     2521 gccggagtcg gcggactatg cctgtattcg ttgtagtgaa atcattcata tgaaagcggg
     2581 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     2641 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     2701 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     2761 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     2821 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     2881 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     2941 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     3001 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     3061 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     3121 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     3181 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     3241 ggttaagcca atgcgtgaat ggcaactctc tgataatgct gcttatatgc attactgccc
     3301 gaatgaaacc atcgacggta tcgccatcga cgaaacgcca gacttcggca aagatgtggt
     3361 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     3421 cgtgatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     3481 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     3541 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     3601 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     3661 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     3721 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     3781 caaattgttc cttgaagagt cttttgctgc tggccttcat gcactgaaag gtcaccgtgt
     3841 ggtcggcgga atgcgcgctt ctatttataa cgccatgccg ctggaaggcg ttaaagcgct
     3901 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     3961 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     4021 tgacgttaca acccatcgct agtgtcgatg gcactattaa tctgcccggt tccaagagcg
     4081 tttctaaccg cgctttattg ctggcggcat tagcacacgg caaaacagta ttaaccaatc
     4141 tgctggatag cgatgacgtg cgccatatgc tgaatgcatt aacagcgtta ggggtaagct
     4201 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     4261 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     4321 cagctctttg tctgggtagc aatgatattg tgctgaccgg tgagccgcgt atgaaagaac
     4381 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     4441 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     4501 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     4561 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     4621 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     4681 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     4741 cttcggcttc ttactttctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     4801 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     4861 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     4921 ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat
     4981 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     5041 gcctgtttgc gatggcaaca gaactgcgta aagtcggtgc ggaagtagaa gaggggcacg
     5101 attacattcg tatcactcca ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     5161 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     5221 ttgatcccaa atgtacggcc aaaacatttc cggattattt cgagcagttg gcgcggatta
     5281 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     5341 cggcacattg tcggcgtttt ttttcggacc ttgtgagtca ttttgattaa tggtagcgtc
     5401 gcttgtcaat gtaagttgtt gatacataat atttatatat gattaatcaa cggataattc
     5461 acatgaagaa tactaaatta ctgctggcga ttgcgacctc tgcagcatta ctgacagggt
     5521 gtcaaaatac ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata
     5581 aagcagcaac attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa
     5641 tggacagcgg caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa
     5701 tcgccaaagc attgggtaac aatattaatg gcacgccggt caactataag gtttatatga
     5761 ccagcgacgt caacgcatgg gcgatggcga acggctgtgt ccgtgtctac agtggcctga
     5821 tggacatgat gaatgacaac gaaattgaag gtgttctggg ccatgaactg ggccacgtcg
     5881 cgttgggtca ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg
     5941 ccatttcagc taccagcgga gtggcttccc agctttcccg ctcacaattg ggtgatatcg
     6001 cagaaggcgc tattaatgct aaatactccc gtgataaaga gtccgaagca gatgatttct
     6061 cctttgatct gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa
     6121 aactggctag tctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa
     6181 cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct
     6241 ttcgggctgg tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag
     6301 acaaaactgg tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg
     6361 cggttatgtt aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt
     6421 attaccattg atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa
     6481 gcattgcaat ggcatctgct ggattcgggt gcaatttatc gcgtactggc attggcggca
     6541 ttacatcacc atgttgatgt tgcgtcagaa gatgcgctgg taccgctggc atcccatctg
     6601 gatgtacgtt ttgtgtcaac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc
     6661 agcggcgaaa ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca
     6721 cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggcctgatt
     6781 gccgatggcc gcgacatggg aacggtggta ttccctgatg cgccagtgaa aattttcctt
     6841 gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt
     6901 agtgttaact ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac
     6961 cgagcggtag cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta
     7021 agcattgagc aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca
     7081 taagcgaccg aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa
     7141 ccccatccgg catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg
     7201 aatcttttgc tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta
     7261 tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga
     7321 aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc
     7381 aggtaggtga cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc
     7441 tgctgtcccg tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg
     7501 aagatgctga aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg
     7561 agctgaacgg tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg
     7621 acactctgca cctggaaggc aaagagcttg aatttaaagt aatcaagctg gatcagaagc
     7681 gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg
     7741 atcagctgct ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca
     7801 ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca
     7861 tggcctggaa acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg
     7921 ttaaagtgct gaagttcgac cgcgaacgta cccgtgtatc cctgggcctg aaacagttgg
     7981 gcgaagatcc gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc
     8041 gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc
     8101 tggtacacgt ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta
     8161 acgttggcga tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct
     8221 ccctgggtct gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca
     8281 agggcgaccg tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc
     8341 tggacggcgg catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg
     8401 aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg
     8461 acgcagaacg tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca
     8521 actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg
     8581 ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag
     8641 catcccgtga ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag
     8701 ctaaattcac cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag
     8761 acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct
     8821 ccaacaacgc aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt
     8881 cgggattttt attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta
     8941 atcaagcact aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa
     9001 tttgccttta aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca
     9061 cccagcaatc gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc
     9121 atatggcctc gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct
     9181 ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac
     9241 tggaaggaaa atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata
     9301 tttacggtta agtttttact caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt
     9361 tcatttctct aatcaggaac tggaagctgc ctcgcagagt tttgagcagt tttcaccctt
     9421 tcgttaaatt cttctgaatg tgcctcgggg aacgcaaaat tcccaccaga caaccgctca
     9481 acaaagttgc acactttcca taaacaggga ggggggcatg aaaataacga cagtcggcgt
     9541 atgcataatt tgcggaattt ttccgttgct gattttgccc caattgcctg ggacattaac
     9601 ccttgcgttt ctgactctct tcgcctgcgt actggcattt atccctgtta aaaccgtccg
     9661 ttatatcgcg ctgacgttgc tgtttttcgt ttggggcata ttatcagcaa agcaaatttt
     9721 gtgggcagga gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcactgcaac
     9781 tgacggcatg accactcatt acggtcaaat tactcatcta caaggtcgac gtatattccc
     9841 tgcgccaggc cttgtgctgt atggcgaata tcttccgcaa gcggtttgtg ctggacaaca
     9901 atggtcaatg aaactcaaag ttcgtgcggt tcatggccaa cttaatgatg gcggctttga
     9961 tagccagcgt tatgccattg cccagcatca accgctcacc ggccgctttc tgcaggcaag
    10021 tgttattgaa ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct
    10081 gcaaccctat ccgtggaatg cggttattct tggtttaggt atgggggaac ggttatccgt
    10141 ccccaaagaa atcaaaaata tcatgcgcga tactggaacg gcgcatttaa tggcgatatc
    10201 gggattgcac atcgcttttg cggcgttgct ggctgccgga ctcattcgca gtggacaaat
    10261 ttttctgcct gggcgctgga tccactggca aataccatta attggtggga tctgctgtgc
    10321 tgctttttat gcctggttga cgggaatgca acctcctgca ttgcgtaccg tggtggcgct
    10381 tgctacgtgg ggaatgctta agttaagtgg gcgacagtgg agtggctggg atgtatggat
    10441 atgttgtctg gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg
    10501 gctctctgcc gctgcggtcg cggcactgat tttttggtac cagtggtttc cctgtcctga
    10561 gtggcaactg ccgccggtat tgcgtgcagt tgtttccctc attcatttgc aactgggaat
    10621 cacactcctg cttatgcctg tgcaaatcgt catatttcat ggcattagtc tgacctcgtt
    10681 tattgcaaat ctattagcaa ttcccttggt gacatttatc acggttccgt tgatcctcgc
    10741 cgcgatggtt gtgcatttaa gcgggccgtt aatcctggag caagggttat ggtttcttgc
    10801 cgaccggtct ttggctttac ttttctgggg gttaaagagt ttgccagaag ggtggatcaa
    10861 cattgctgaa cgttggcaat ggctatcatt ttccccatgg ttcttactgg tggtatggcg
    10921 attaaacgcc tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctg
    10981 gccgctgtgg caaaaacctc gacctgacga gtggcaggtg tacatgcttg atgtcgggca
    11041 agggctggca atggtgatag ccagaaacgg caaagcgatt ctctatgaca caggactggc
    11101 ctggcccgaa ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct
    11161 tgaaccggaa ggcgttattc tgagtcatga acatctggat caccggggag ggctggactc
    11221 aatattgcac acatggccga tgttatggat cagaagtccg ttaaactggg aacatcatca
    11281 gccctgtgtg cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg tgcactggcc
    11341 tttacaagct agcaacgata aaggaaataa ccattcctgt gtggttaagg ttgatgacgg
    11401 gacgaatagc attcttctaa ccggtgatat tgaagtcccc gctgaacaaa agatgctaag
    11461 ccgttactgg cagcaagtgc agacaacatt gcttcaggta cctcaccatg gcagtaatac
    11521 ctcatcatcg ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc
    11581 gcgctataac gcatggcgat tgccctctaa taaagttaag catcgctatc aacagcaagg
    11641 atatcaatgg cttgatactc cacatcaggg tcaagtgacg gtcaattttt cagcgcaagg
    11701 ctggcggatt agcagcctca gagagcaaat tttacctcgt tggtatcatc agtggtttgg
    11761 cgtgccagtg gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat
    11821 gcataacgac aaagatctct ctacgtggca gacgttccgc cgactgtggc caaccattgc
    11881 gccttttaaa gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga
    11941 taccttcatg ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg
    12001 ctccgtgctg gtgtggatgc cgctggtggt gatcgggctg atgattttac gtggtatcac
    12061 cagctatgtc tccagctact gtatctcctg ggtatcagga aaggtggtaa tgaccatgcg
    12121 tcgccgcctg tttggtcaca tgatgggaat gccggtttca ttttttgaca aacagtcaac
    12181 ggggacgctg ttgtcacgta tcacctacga ttccgaacag gttgcttctt cttcttccgg
    12241 cgcactgata acggttgtgc gtgaaggtgc gtcaatcatc ggcctgttca tcatgatgtt
    12301 ctattacagt tggcaactgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc
    12361 gattcgcgta gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg
    12421 gcaggtgacc accagcgccg aacaaatgct gaagggccac aaagaagtat tgattttcgg
    12481 tggtcaggaa gtggaaacga aacgctttga taaagtcagc aaccgaatgc gtcttcaggg
    12541 gatgaaaatg gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt
    12601 ggcgctggcg tttgttctgt atgcggcgag cttcccaagt gtcatggata gcctgactgc
    12661 cggtacgatt accgttgttt tctcttcaat gattgcactg atgcgtccgc tgaaatcgct
    12721 gaccaacgtt aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat
    12781 tctggacagt gagcaggaga aagatgaagg taagcgcgtg atcgagcgtg cgactggcga
    12841 cgtggaattc cgcaatgtca cctttactta tccgggacgt gacgtacctg cattgcgtaa
    12901 catcaacctg aaaattccgg cagggaagac ggttgctctg gttggacgct ctggttcggg
    12961 taaatcaacc atcgccagcc tgatcacgcg tttttacgat attgatgaag gcgaaatcct
    13021 gatggatggt cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct
    13081 ggtgtcgcag aatgtccatc tgtttaacga tacggttgct aacaacattg cttacgcacg
    13141 gactgaacag tacagccgtg agcaaattga agaagcggcg cgtatggcct acgccatgga
    13201 cttcatcaat aagatggata acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct
    13261 ctctggcggt cagcgtcagc gtattgctat cgctcgagcc ttgctgcgtg atagcccgat
    13321 tctgattctg gatgaagcta cctcggctct ggataccgaa tccgaacgtg cgattcaggc
    13381 ggcactggat gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac
    13441 cattgaaaag gcagacgaaa tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac
    13501 gcataacgat ttgcttgagc accgcggcgt ttacgcgcaa cttcacaaaa tgcagtttgg
    13561 ccaatgatcg aaaaaatctg gtctggtgaa tcccctttgt ggcggctatt gctgccactc
    13621 tcctggttgt atggcctggt aagtggcgcg atccgtcttt gctataaact aaaactgaag
    13681 cgcgcctggc gtgcccccgt accggttgtc gtggttggta atctcaccgc aggcggcaac
    13741 ggaaaaaccc cggtcgttgt ctggctggtg gaacagttgc aacagcgcgg tattcgcgtg
    13801 ggggtcgtat cgcggggata tggtggtaag gctgaatctt atccgctgtt attgtcggca
    13861 gataccacaa cagcacaggc gggtgatgaa cctgtgttga tttatcaacg cactgatgcg
    13921 cctgttgcgg tttctcccgt tcgttctgat gcggtaaaag ccattctggc gcaacaccct
    13981 gatgtgcaga tcatcgtaac cgacgacggt ttacagcatt accgtctggc gcgtgatgtg
    14041 gaaattgtcg ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg
    14101 ccaatgcgtg agcgagcggg gcgcttaaag tcggttgatg cggtaatcgt caacggtggt
    14161 gtccctcgca gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt
    14221 accggtacgc gttgtgacgt tgctcagctt gaacatgtag tggcgatggc ggggattggg
    14281 catccgccgc gcttttttgc cacgctgaag atgtgtggcg tacaaccgga aaaatgtgta
    14341 ccgctggccg atcatcagtc tttgaaccat gcggatgtca gtgcgttggt aagcgccggg
    14401 caaacgctgg taatgactga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat
    14461 tggtggtatt tgcctgtaga cgcacagctt tcaggtgatg aaccagcgaa actgcttacg
    14521 caactaacct cgctggcttc tggcaactag ttacgccgcg gcagcgttcg attgatggag
    14581 tcatgaatgt cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgca
    14641 caaggcctgt taaacaaacc ccgccgtcaa gcgtcgttgg aggatattcc ggcaacgatc
    14701 tcccgcatgt ccttgctgca aatcgatacc atcaatattg ttgcccgtag tccatatctg
    14761 gtgcttttca gtcgtctggg aaattatcct gcccagtggc tggatgagtc tctggcgcgt
    14821 ggcgaattaa tggaatactg ggcgcatgaa gcctgcttta tgccgcgtag cgactttcgt
    14881 cttattcgcc accgcatgct ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg
    14941 atgcaggaac atgaggcgga aattgcacag ttaattcagc atattcatga taaggggccg
    15001 gtacgttcag ccgattttga gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag
    15061 ccgcataaac ggcatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg
    15121 cgcaacttcc agcgcgttta tgatttaacc caccgtgtca tgcctgactg ggatgatgag
    15181 cgcgatctcg tttcgcaaac agaagcagaa atcatcatgc tggataacag tgcgcgtagc
    15241 ctgggaatat tccgcgaaca gtggctggca gattactatc ggctgaaacg tccggcactg
    15301 gcggcgtggc gcgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa
    15361 ttgggcaatc tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctggccgga
    15421 aagctcactg caacgcacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc
    15481 aaacgcgcag agcagctttt tgattttagc taccggctgg agtgctatac cccagcgccg
    15541 aaacgccagt atggctattt tgttctgcca ttattacatc gtgggcaatt agttgggcga
    15601 atggatgcca aaatgcatcg ccagacaggc atccttgaag ttatctctct gtggttacag
    15661 gaaggcatta aaccaacgac aatgctgcaa aaagggttac gtcaggcgat tactgatttc
    15721 gcgagctggc agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg tctctttact
    15781 gattgccgca ctggctggga aatagacccc gtcgcataaa tgattatgat aagatttatg
    15841 cattcataac ccgatccatc tggaggaact atggatcatc gtctgcttga aatcattgcc
    15901 tgcccggttt gcaacggaaa actttggtat aaccaagaaa aacaagaact tatttgcaaa
    15961 ctggacaacc tggctttccc gctgcgtgat ggcattccag tgttgctgga aacggaagcc
    16021 cgcgtgctga ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgct
    16081 acgcgtcaac gcgtctgccc ggtaaaccat tggttgatat taatggcaaa cccatgattg
    16141 ttcatgttct tgaacgtgct cgcgaatcag gtgccgatcg catcatcgtg gcaaccgatc
    16201 atgaggatgt cgcccgcgcc gttgaagccg ctggcggtga agtatgtatg acgcgcgccg
    16261 atcaccagtc agggacagaa cgcctggcgg aagttgtcga aaaatgcgca ttcagcgacg
    16321 acacggtgat cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc
    16381 aggttgctga taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc
    16441 acaatgcgga agaagcgttt aacccgaatg cggtgaaagt ggttctcgac gctgaagggt
    16501 atgcactgta cttctctcgc gccaccattc cttgggatcg tgatcgtttt gcagaaggcc
    16561 ttgaaaccgt tggcgataac ttcctgcgtc atcttggtat ttatggctac cgtgcaggct
    16621 ttatccgtcg ttacgtcaac tggcagccaa gtccgttaga acacatcgaa atgttagagc
    16681 agcttcgtgt tctgtggtac ggcgaaaaaa tccatgttgc tgttgctcag gaagttcctg
    16741 gcacaggtgt ggatacccct gaagatcttg agcgcgttcg cgctgaaatg cgctaatttc
    16801 acttcacgac acttcagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat
    16861 ttttcttttc aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga
    16921 aagcagtagc ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt
    16981 tactgggcga aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt
    17041 gggctttgta tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc
    17101 ccggaaaagc ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc
    17161 gtatgccgac tatttatggt gtgatgacgc atgaagaaca ccccggcccc gatgtcctgc
    17221 tactggagcg gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg
    17281 aacaactcaa agaccaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg
    17341 gttgcgtcgg cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac
    17401 atgttgaagt gctatggacc acgctcaatc agttcaataa caccggtctg acgatgcagg
    17461 ataagcggat cctgtttcgc actcgcgaat gtctcccggc attatttgaa ggctttaacg
    17521 acaattgtgt gctgattcac ggtaacttct gtttacgcag catgttgaaa gattcgcgca
    17581 gcgatcagtt actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg
    17641 aactgttccg actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac
    17701 gcgcgccagt ggcggagtcg ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag
    17761 ttgcgcaact ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat
    17821 cactcttgcc gtggctcgcc tgacgaacct tttagccact gccagatacg gccaagcgtt
    17881 tcatagccaa cacgatcgct atgcatcaac cagaccggag aagggattgc ccgttcccac
    17941 gggttaagcg gcgagtcgat ggccaactga ttcgccgggg ctggtagcgg atttaaccct
    18001 tcctgctgaa aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa
    18061 ggggcgtcac caatcgcctg tttcactgcc gcagcttctt cttcggtatc ttttggcaaa
    18121 tccagggtga taatttgctc gtgcggcacg cccagcgatt gcgcaactct ggcacctact
    18181 tccgctgtac tcaccgtatt ggtttttgct acgcctcccg tgaagatcag ttttgatccc
    18241 ggattttcgc gccataagcg aataccttca ttcaggcgtg gcagactgtt attgattaaa
    18301 ttagagctcg gtgcccactg tgggttccag gtataaccac ctccgagcac cacgatatag
    18361 tccacttttt gcgaattatt ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg
    18421 tcggctaccg gttgcaaact caataacaag agtgccagcc acccaatgct aataaaaatc
    18481 ttcccggttt tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataattaac
    18541 aacatcagcg gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc
    18601 ctttctggtt ccttttttaa ccatatagca agggatcgcc agcgatatta caccagacgg
    18661 gttcattctc gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac
    18721 cggtggagaa agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta
    18781 acatttacgg caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc
    18841 acgtgctggc ggaaatgggc ccgcaaaaac tgcgtgtgct ggatgctggc ggtggtgaag
    18901 ggcagaccgc aatcaaaatg gccgagcgtg ggcatcaggt cattttatgc gatctttctg
    18961 cgcagatgat cgaccgcgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc
    19021 aatttataca ttgcgccgct caggatgttg cttcgcattt ggaaacgccc gttgatctga
    19081 tattgttcca tgcggtgctc gagtgggtgg ccgatccccg cagtgtattg cagaccctgt
    19141 ggtcagtgtt gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt
    19201 tgatgcataa catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga
    19261 aaaaacggac gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg
    19321 aagaagctgg ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc
    19381 gcgagaagca ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt
    19441 gccgtcagga accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc
    19501 agagcaagga taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc
    19561 cagaaaaaat gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt
    19621 tgccacgctg aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc
    19681 attccgccat gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa
    19741 taacgcgatc aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc
    19801 ggaagggaac gcaatttacc gtctgacgcc gctcggcatc ggcattactg actactacat
    19861 ccgtcagcgc gagttttcta cgctgcgtct ttctatgcag ttgtcgattg tggcgggtga
    19921 gctcaaacgc gcagcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa
    19981 tgtctatgcg ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca
    20041 acgtctgatg gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa
    20101 agactggcgg gcggcgattt ccagctgtga attgttgctt tcggaaactt ccgggacgct
    20161 gcgtgaattg caggatacgc tggaagcggc aggcgacaaa ttgcaggcta atctgttgcg
    20221 cattcaggat gcgacgatga cccatgacga tctgcatttc gtcgatcgtc tggtgttcga
    20281 tctgcagagc aaactcgatc gtattatcag ttggggccag caatccatcg acttgtggat
    20341 tggctacgac cgccacgtac acaaatttat tcgtaccgcg atcgatatgg ataaaaaccg
    20401 cgtctttgct cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct
    20461 aacttatgcc aatgccgatc gtctgctgga tatgcgtgac gaagagatgg cactgcgcga
    20521 tgaagaagtg actggggaac ttcctgagga tctggaatac gaagagttta acgagatccg
    20581 cgaacagctg gcggcgatca tcgaagaaca acttgccgtg tacaaaacca gacaagtgcc
    20641 gctggatctt ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt
    20701 tgacgttgcg cgtattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt
    20761 caccggactg ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca
    20821 tgtcatcgac aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc
    20881 cgttatttcc ggcgctggac agcgccttac gttcaggccg ccatattggc ctcgacgaac
    20941 tggataatca tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt
    21001 ataacgttga gcttattcgc gcaccagaag ggttcttcta tttacgccca cgttccacca
    21061 cgctgatccc tcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt
    21121 atctctatct cagcccggaa cggctggcga atgaggggat tttcacccag caggaactgt
    21181 acgacgaact gctcaccctg gccgatgaag caaaactgct gaaactggtg aacaaccgtt
    21241 caaccggttc agacgttgac cgtcagaagt tgcaggagaa agtacgttct tcgctcaacc
    21301 gtctgcgtcg tttaggcatg gtgtggttta tgggccacga cagcagcaag tttcgcatta
    21361 ccgaatcggt gttccgcttc ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac
    21421 agcgtcgcct gattcgtgat ggcgaagcaa tgccgattga aaatcatctg caactcaacg
    21481 atgaaaccga agaaaatcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa
    21541 atttcgctca ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga
    21601 cgagctggtc acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc
    21661 gttcgttacg gcgctgatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc
    21721 cggggccacc agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg
    21781 ttattcgatg ctcgacacca ttaactcgcg ccaccagcgc gtggtggtcg gtgtgcgtct
    21841 gcaacaggtt gccggacgcg atcgtaaagt ggatatcaag ccgtttgcca ttcagggact
    21901 gccgatgtcg gtgcagccga cacagctggt gaccgaaacc ctgaacgaac gccaggcgcg
    21961 cgtgctgccg cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa
    22021 acagttcaac tccattactg attaccactc gctgatgttc gatctgggca tcatcgcgcg
    22081 tcgtctgcgc tccgcatctg accgtagcaa attctatcgt ctgatcgaag cttcgctgta
    22141 tggcgggatc tccagtgcca ttacccgttc tctgcgcgac tacctgttgc cggaaaacag
    22201 cggcgtgcgt aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gtatgacgct
    22261 ggaagcgatt cgtgtcaccc agtcggaccg tgacctgttt aagcatctga tcagcgaagc
    22321 taccaactac gtggcggcgg actacatgcg tcacgccaac gagcgccgtg tccatctcga
    22381 caaagccctg gagtttcgtc gcgagttaca tacttcgcgt cagcaactgg cggctgagca
    22441 gtacaaacac gtcgatatgg cgcgtgagct ggcagagcac aacggtgccg aaggcgatct
    22501 ggaagcggat tatcaggcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca
    22561 gcaggagaag atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga
    22621 gcaaaacgaa gtggtggcag aagccatcga acgccaggaa gagaacgagg ctcgcgcgga
    22681 agctgccgaa ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct
    22741 ggacgtccag caaacgcgcg cgatccagta taaccaggcg attgctgcgc ttaatcgcgc
    22801 caaagaactg tgccatctgc cggacttaac cgccgacagc gccgccgaat ggctggaaac
    22861 cttccaggcg aaagagctgg aagcgactga aaaaatgctc tctcttgagc agaaaatgag
    22921 catggcgcaa accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa
    22981 cggcccgctg gcgcgtaacg aggcgtggga tgtcgctcgc gaactattgc gcgaaggggt
    23041 cgatcagcgt cacctggcag agcaggttca accgttgcgg atgcgcttaa gtgaactgga
    23101 acagcgtctg cgcgagcagc aagaagccga acgcctgctg gcagacttct gcaaacgtca
    23161 gggcaagaat tttgatattg atgaactgga agctctgcat caggagctgg aagcgcgcat
    23221 tgcctctctt tccgatagcg tgtctaacgc ccgtgaagag cgcatggcac tgcgccagga
    23281 acaggaacag ctgcagtctc gcattcagag tttgatgcag cgtgcgccgg tttggctggc
    23341 agcgcaaaac agtctcaacc agttgagcga gcagtgcggt gaagagttta cctccagcca
    23401 ggacgtcacc gaatatctgc aacagttgct ggagcgtgag cgagaggcga ttgttgaacg
    23461 cgatgaagtg ggcgcgcgca aaaacgctgt cgatgaagag atcgaacgtt taagccagcc
    23521 tggcggctct gaagatcagc gtctgaacgc gctggcggag cgttttggcg gcgtgctgct
    23581 gtcagaaatt tatgacgacg ttagcctgga agatgcgccg tacttctcag cgctgtatgg
    23641 cccgtcacgc cacgccatcg tggtgccaga tctgtcacag gtaactgaac acctggaagg
    23701 cttgaccgat tgcccggaag atctctatct gatcgaagga gatccgcagt cattcgatga
    23761 cagcgtgttc agcgttgatg agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca
    23821 gtggcgttat tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg
    23881 tattgaaagc ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt
    23941 tgatgtacag aaaactcagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct
    24001 ggcggttgcg tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt
    24061 cgaactggag cgggcgttga gtaatcatga aaatgataac cagcagcagc gtattcagtt
    24121 tgagcaggcg aaagaaggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt
    24181 ggctgatgac agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca
    24241 ggaagccgcg cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgatcgt
    24301 ttcggtattg cagagcgacc cggaacagtt cgaacagcta aaagaagatt acgcgtactc
    24361 tcagcagatg cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg
    24421 tcgtgcgcac tttagctatt ctgactcggc agaaatgctg agcggtaata gcgatctcaa
    24481 cgaaaagctg cgtgaacgtc tggaacaggc ggaagcggag cgtactcgcg ctcgtgaagc
    24541 gttgcgtggt cacgcagcgc agttgagtca gtacaaccag gtgctggctt cgctgaaaag
    24601 ttcttacgac accaaaaaag agctactcaa cgatctgcaa cgtgaattgc aggatatcgg
    24661 cgtgcgcgcc gatagcgggg cagaagagcg ggcgcgaatt cgccgtgacg agctgcatgc
    24721 gcaactgagt aataaccggt cccgccgcaa ccagctggaa aaagcactga ctttctgcga
    24781 agcggagatg gacaatctga cccgcaaact gcgcaagctg gagcgggatt actttgagat
    24841 gcgcgagcag gtagtgaccg ccaaagcggg ctggtgtgca gtgatgcgca tggtgaaaga
    24901 taacggcgtt gaacggcgct tacaccgtcg cgagctggct tatctctccg ctgatgattt
    24961 gcgctctatg tcggataagg cattgggtgc gctgcgtctg gcggtggcgg ataacgaaca
    25021 tctgcgcgac gtgctgcgca tgtcggaaga tccaaaacgt ccggaacgta aaattcagtt
    25081 cttcgtggcg gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga
    25141 tgatccggtg gaagccatcg agcagatgga aatcgaactt agccgactga ccgaggagtt
    25201 aacctcccgc gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa
    25261 aaccattcag cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc
    25321 gtttggtcag gtgaacagcg tacgtctgaa cgtgaacgtg cgtgaaacgc acgccatgct
    25381 gctggatgtg ctctctgaac agcacgagca gcatcaggat ctgtttaaca gcaaccgtct
    25441 gaccttctcg gaagcgctgg cgaaattgta tcagcgtctt aatccgcaga ttgatatggg
    25501 gcagcgcacg ccgcagacca ttggcgaaga actgctggat taccgcaact atctggaaat
    25561 ggaagttgag gttaatcgtg gttccgatgg ctggttgcgc gcagagtctg gtgcattgtc
    25621 gaccggtgag gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga
    25681 agatgaatct cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga
    25741 tgaagcagcg cgactggatg ctcgttctat cgccacgctg tttgaattgt gtgaacgttt
    25801 gcaaatgcag ctcatcatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta
    25861 taaactggtg cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg
    25921 atttgcgccg caactccctg aaacgcttcc aggaagtgac gaagcgcctt ctcaggcgag
    25981 ttaaaattaa ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttctgcact
    26041 aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc tttacattag gttatgtaaa
    26101 aacgtatcgg cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata
    26161 ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag
    26221 tatgataaaa acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg
    26281 gcaatcagtt tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag
    26341 cctgaagtaa tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg
    26401 caggcgcaag ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggcagaa
    26461 aaagcccgca cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac
    26521 cagcttcaac tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct
    26581 gttaaagcct tccagcaaca gctggcagag gtggcgattg ccggtttcca gccgcagttt
    26641 aataaatggg tagagttact gaccgatcct ggtgttaacg ggatggcacg cgacgtagtg
    26701 ctctctgatg cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact
    26761 cgctggctat atagcagtaa accttatgca cttgcaacgc cgccgctctc ggtgattaac
    26821 caatggcagc tggcgctgga taaaggtcaa ttgcctacgt ttgttgctgg actggcaccg
    26881 cagcatccgc aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa
    26941 ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta
    27001 ccggcgttgc gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact
    27061 ctacctggcg atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa
    27121 acagcagaaa ctaagccgat ggacaagcaa acgacatctc gtagtaaacc tgcgcctgcc
    27181 gttcgcgccg cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa
    27241 ggattggggg cagatggtgc tattggcccg gcaacacgtg actggttaaa cgtaacgccc
    27301 gcccagcgtg ctggcgtgtt ggctctcaac atccagcgat tgcgcttgct gccaacagag
    27361 ctttctaccg ggatcatggt taacattccg gcctattcgc tggtctacta tcagaacggc
    27421 aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg
    27481 agcagtgccc ttaacaacgt agtggtaaac ccgccgtgga acgtacctcc aactctggca
    27541 cgcaaagata ttctgccaaa agtgcgcaac gatccgggat atctcgaaag ccatggctat
    27601 acggtgatgc gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct
    27661 acaatcacgg cctcgaattt accgttccgc ttccagcagg ctccaggccc acggaactcg
    27721 ctggggcgct ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg
    27781 aaccacaatc tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat
    27841 aaagcttccg atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt
    27901 tctgatgcgc tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg
    27961 aatctctact acctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat
    28021 atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa
    28081 ttaatcaggt aaatgaagta gttcggggaa atcagttgtc gtaataatta gcatgattgg
    28141 gggcgattct ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac
    28201 gggcggttaa ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta
    28261 gacttgatta tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc
    28321 gttgcactcg gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc
    28381 ccgcgcattt tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc
    28441 gatggcagag gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac
    28501 cgcgcgaaca aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa
    28561 gggttgttag gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc tattgatacc
    28621 aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc
    28681 caggcgatgg atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta
    28741 tctatgcgcg caggtggtgt aggatattat ccacgtagta actttgtgca tattgatacc
    28801 gggccagcac ggcactggta gtaattgctt aacgaaacag gggcagtatg aactatcgta
    28861 ttattccggt caccgcattc tcccagaact gttcattaat ctggtgtgaa caaacccgtc
    28921 tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca
    28981 gcggcctgac actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag
    29041 cggcggaact ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt
    29101 tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc
    29161 tgacgccaga tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac
    29221 aggtgttaca ttgccctggg catacgccgg gtcatgtcgt gttttttgat gatcgggcaa
    29281 agctgctgat ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc
    29341 gtggcgatca taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg
    29401 acgttacatt tattccgggt cacggaccat tatccacact tggttatgaa cgcctgcata
    29461 atcccttcct gcaagacgaa atgcccgtct ggtaaggcac ataaaaaagc ccgcttttaa
    29521 tgcgggcctg gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct
    29581 tatccggcct acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa
    29641 gcgtagcgca tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct
    29701 tcattgtttt taatgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat
    29761 ctggtgtcat cccggccaca tttacgcgac cagaagcaac cgcatatacg ccaaactctt
    29821 cgcgcagacg cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt
    29881 tgatgataaa gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact
    29941 gacgcatacg ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta
    30001 acgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt
    30061 agttagcgcg aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg
    30121 cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg
    30181 aactggcaac aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt
    30241 cttccagacc acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct
    30301 caactgagag ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt
    30361 tatggcagca gccatggaac agcactacgt cgccagcctg agcttcattc aggctgttaa
    30421 tcagtgcatc gaagtcaaga gtgtgatttt ccgcatcata ataagcgtat tcacgaactt
    30481 ccagacctgc agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca
    30541 ctcgcttaac gctggtattt tttgccagga aatcggcagc tacgcgtagt gcgccagtgc
    30601 cccccggagt ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa
    30661 acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt
    30721 tggtggtttc attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg
    30781 ttttgcccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat
    30841 cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct
    30901 caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga
    30961 gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga
    31021 ttgctctgga aggtataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa
    31081 gaggtgtgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg
    31141 cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt
    31201 ttgttgaagt agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata
    31261 ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg
    31321 aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt
    31381 gtaaatttat tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag
    31441 atgttgttcg cgtcgtactt cagaccagta gcccactgtt cagctttttt accgttgcca
    31501 agaggttgag cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca
    31561 aagccttcgt attcgtagct gatagaaccg ccaacaccgt cgccgttaga acggcgtgca
    31621 gtgtcacgct cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca
    31681 aagaagttgg agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg
    31741 ctgtatgcag tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa
    31801 accacaccgt agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg
    31861 aatgccagac gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg
    31921 aagttatatt cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta
    31981 aaaccaagac gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc
    32041 ttggaaaaat aatgcagacc aacagcttta ccgtacagat ctactttgtt gccatctttg
    32101 ttatagattt ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc
    32161 agaatattgc gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac
    32221 tgccttcaat aagttctgtc aataaaaatt tacggaacta ttgatgagag tttggtgtct
    32281 ttatgtgtct gcaggcatct ttccattca
//
LOCUS       DAFAVF010000039        32073 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFAVF010000039 DAFAVF010000000
VERSION     DAFAVF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.039"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            258..2963
                     /gene="malT"
                     /locus_tag="LGK42_001789"
     CDS             258..2963
                     /gene="malT"
                     /locus_tag="LGK42_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBK2658046.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICHDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3006..4022)
                     /gene="rtcA"
                     /locus_tag="LGK42_001790"
     CDS             complement(3006..4022)
                     /gene="rtcA"
                     /locus_tag="LGK42_001790"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBK2658047.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4026..5252)
                     /gene="rtcB"
                     /locus_tag="LGK42_001791"
     CDS             complement(4026..5252)
                     /gene="rtcB"
                     /locus_tag="LGK42_001791"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBK2658048.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5441..7039
                     /gene="rtcR"
                     /locus_tag="LGK42_001792"
     CDS             5441..7039
                     /gene="rtcR"
                     /locus_tag="LGK42_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBK2658049.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7021..7779)
                     /locus_tag="LGK42_001793"
     CDS             complement(7021..7779)
                     /locus_tag="LGK42_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBK2658050.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7796..8626)
                     /gene="glpG"
                     /locus_tag="LGK42_001794"
     CDS             complement(7796..8626)
                     /gene="glpG"
                     /locus_tag="LGK42_001794"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBK2658051.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8671..8997)
                     /gene="glpE"
                     /locus_tag="LGK42_001795"
     CDS             complement(8671..8997)
                     /gene="glpE"
                     /locus_tag="LGK42_001795"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBK2658052.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9187..10692
                     /gene="glpD"
                     /locus_tag="LGK42_001796"
     CDS             9187..10692
                     /gene="glpD"
                     /locus_tag="LGK42_001796"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBK2658053.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYIWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10744..11349)
                     /locus_tag="LGK42_001797"
     CDS             complement(10744..11349)
                     /locus_tag="LGK42_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658054.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGNWVTCLSLAILAQGPLFNYLQ"
     gene            complement(11352..12119)
                     /locus_tag="LGK42_001798"
     CDS             complement(11352..12119)
                     /locus_tag="LGK42_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658055.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(12122..12832)
                     /locus_tag="LGK42_001799"
     CDS             complement(12122..12832)
                     /locus_tag="LGK42_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658056.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVLKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(12847..14355)
                     /locus_tag="LGK42_001800"
     CDS             complement(12847..14355)
                     /locus_tag="LGK42_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK2658057.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(14480..16927)
                     /gene="glgP"
                     /locus_tag="LGK42_001801"
     CDS             complement(14480..16927)
                     /gene="glgP"
                     /locus_tag="LGK42_001801"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBK2658058.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16946..18379)
                     /gene="glgA"
                     /locus_tag="LGK42_001802"
     CDS             complement(16946..18379)
                     /gene="glgA"
                     /locus_tag="LGK42_001802"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBK2658059.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18379..19674)
                     /gene="glgC"
                     /locus_tag="LGK42_001803"
     CDS             complement(18379..19674)
                     /gene="glgC"
                     /locus_tag="LGK42_001803"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBK2658060.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(19692..21665)
                     /gene="glgX"
                     /locus_tag="LGK42_001804"
     CDS             complement(19692..21665)
                     /gene="glgX"
                     /locus_tag="LGK42_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBK2658061.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(21662..23848)
                     /gene="glgB"
                     /locus_tag="LGK42_001805"
     CDS             complement(21662..23848)
                     /gene="glgB"
                     /locus_tag="LGK42_001805"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBK2658062.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24121..25224)
                     /gene="asd"
                     /locus_tag="LGK42_001806"
     CDS             complement(24121..25224)
                     /gene="asd"
                     /locus_tag="LGK42_001806"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK2658063.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25417..26010
                     /gene="yhgN"
                     /locus_tag="LGK42_001807"
     CDS             25417..26010
                     /gene="yhgN"
                     /locus_tag="LGK42_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBK2658064.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26067..27407)
                     /gene="gntU"
                     /locus_tag="LGK42_001808"
     CDS             complement(26067..27407)
                     /gene="gntU"
                     /locus_tag="LGK42_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBK2658065.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(27411..27938)
                     /gene="gntK"
                     /locus_tag="LGK42_001809"
     CDS             complement(27411..27938)
                     /gene="gntK"
                     /locus_tag="LGK42_001809"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK2658066.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28077..29072)
                     /gene="gntR"
                     /locus_tag="LGK42_001810"
     CDS             complement(28077..29072)
                     /gene="gntR"
                     /locus_tag="LGK42_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBK2658067.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(29296..29991)
                     /gene="yhhW"
                     /locus_tag="LGK42_001811"
     CDS             complement(29296..29991)
                     /gene="yhhW"
                     /locus_tag="LGK42_001811"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBK2658068.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30114..31151)
                     /locus_tag="LGK42_001812"
     CDS             complement(30114..31151)
                     /locus_tag="LGK42_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK2658069.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            31484..31972
                     /gene="yhhY"
                     /locus_tag="LGK42_001813"
     CDS             31484..31972
                     /gene="yhhY"
                     /locus_tag="LGK42_001813"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2658070.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7939 a         8662 c         7987 g         7485 t
ORIGIN      
        1 ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac ataagggaaa
       61 ccagtattca cgctggatca gcgtcgtttt aggtgagttg ttaataaaga tttggaattg
      121 tgacacagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag aaaggtttct
      181 ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc tcattttcca
      241 cagtgaagtg attaactatg ctgattccgt caaaactaag tcgtccggtt cgactcgacc
      301 ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac ttccggctgg
      361 cgctgatcac gagtcctgcg ggctacggaa agaccaccct catttcccag tgggcggcag
      421 gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa gagcgtttcg
      481 ccagctatct cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga
      541 cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag cttttcattg
      601 agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat ctgatcacta
      661 atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa aatctcaccc
      721 tggtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt gttcgtgatc
      781 aactgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt
      841 ttgattgccg tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt tgccatgacg
      901 tttccggttg ggcgacggca ctacagctaa tcgccctctc cgcccggcag aatacccact
      961 cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc
     1021 tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg
     1081 ccattttgcg ctcaatgaac gatgcactca tcacccgtgt gaccggcgaa gaaaacgggc
     1141 aaatgcgcct cgaagagatt gagcgtcagg ggctgttttt acagcggatg gatgataccg
     1201 gcgagtggtt ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg
     1261 aactggcggc ggagctgccg gaaatccacc gtgccgccgc agaaagctgg atggcccagg
     1321 gatttcccag cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg
     1381 atattctgct taatcacgcc tggagtttgt tcaaccatag cgaactgtcg ctgctggaag
     1441 agtcgcttaa ggccctgccg tgggacagtt tgctggaaaa tccgcagtta gtgttattgc
     1501 aggcgtggct aatgcaaagc caacatcgct atggcgaagt taacaccctg ctagcccgtg
     1561 ctgaacatga aatcaaggac atcagagaag gcaccatgca cgcagaattt aacgctctgc
     1621 gcgcccaggt ggcgattaac gatggtaatc cggatgaagc ggaacggctg gcaaaactgg
     1681 cactggaaga gctgccgccg ggctggttct atagccgcat tgtggcaacc tcggtgctgg
     1741 gtgaagtgct gcactgcaaa ggcgaattga cccgctcact ggcgctaatg cagcaaaccg
     1801 aacagatggc acgccagcac gacgtctggc actacgcctt atggagttta atccagcaaa
     1861 gtgaaattct gtttgcccaa gggttcctgc aaaccgcgtg ggaaacacag gaaaaagcgt
     1921 tccagctgat caacgagcaa catctggaac agctgccgat gcatgagttt ctggtgcgca
     1981 tccgagcaca actgttatgg gcctgggcac ggctggatga agccgaagcg tcggcgcgta
     2041 gcgggattga agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt
     2101 tgattcaatg ctcgctggcc cgtggtgatt tagataacgc ccgtagccag ctgaaccgtc
     2161 tggaaaacct gctggggaat ggcaaatatc acagcgactg gatctctaac gccaacaaag
     2221 tccgggtgat ttactggcaa atgaccggcg ataaagccgc cgctgccaac tggttgcgtc
     2281 atacggctaa accggagttt gcgaacaacc acttcctgca aggtcaatgg cgcaacattg
     2341 cccgcgcaca aatcttgctg ggcgagtttg aacctgcgga aattgttctc gaagaactca
     2401 atgaaaatgc ccgcagtctg cggttgatga gcgatctcaa ccgtaacctg ttgctgctta
     2461 atcaactgta ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat
     2521 taaaactggc gaatcgcacc ggatttatca gccattttgt catcgaaggc gaagcgatgg
     2581 cgcaacaact gcgccagctg attcaactca atacgctgcc ggaactggaa cagcatcgcg
     2641 cacagcgtat tctgcgagaa atcaatcaac atcatcggca taaattcgcc catttcgatg
     2701 agaatttcgt tgaacgtctg ctgaatcatc cggaagtgcc agaactgatc cgcaccagcc
     2761 cgttgaccca acgtgaatgg caggtcctgg ggctgattta ctccggttac agcaatgagc
     2821 aaatagcggg tgaactggaa gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt
     2881 accaaaaact cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag aaactgctga
     2941 agatgatggg ttatggggtg tgagtttagc cggataatgc attggatccg gtttacatct
     3001 ttacatcatt caatactcac ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc
     3061 ggcaagaaac gctccaccac cgcgatattg gtcagcagat ggcatgaagg atgggcgacc
     3121 ttaaattctc ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc gagatattcc
     3181 cccaccgccg ccgggcttgc caggtagcgt ttcacctctt tcactaactg cgcggctacc
     3241 acctcggcac tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat attttcactt
     3301 tcgacttcca gcgagacggt attacccggc ccctggtcgc gcggcaggtt atgaatattc
     3361 tgttcatgca gggaaaaact ccccgccagt gtagcgattt cacgctcagc aacatggcgc
     3421 ggcacaccag ctaatagaac ttctccacgc atctgcacaa tgttcccgcg ctcgccaagt
     3481 tgcaaggtgt taaacgatgc cacgggcgag acttccgttg ccaccacgcc gcctccggca
     3541 ggataaaaac cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc
     3601 ggctccagca cccggcggat aaaatccgca ggcggggccg acgggttatc ggtgcctccg
     3661 ctcacttcaa cacgcgaagg tccatcggca aaccacagcg cgggcagcac cgtttgcagc
     3721 accagcgtac agcttccggc actaccgata gcaaagcggt actcgccgcc gcgcacggtg
     3781 ccaggccgga agaccagacg ctgcgacccc agctccgcgc cttccaccgt tgccctacaa
     3841 atttccgcag ccgcttttac cgcggtcaga tgctggcgca acagccccgg tttcgcccgc
     3901 ccggcacgaa tgctggtgat ggtaaatggc tggccggtta tcatcgacag gctcagcgcc
     3961 gagcgcagga tctgcccgcc accttcgccc tgtgcgccat ccagcgcaat catccttttc
     4021 atcatttatc cttttacgca caccacctga cgcagggtat agataacttc caccagatcg
     4081 ctttgtgccg ccatcaccgc atcaatatct ttatacgcca tcgggatttc gtcgatcact
     4141 tcggcatctt tacggcattc cacatgcgcg gtggcacgaa tttgatcttc cacgctgaac
     4201 agttttttcg ctttagtacg gctcattacc cgcccggcac cgtggctgca cgaacagaac
     4261 gactcttcat ttcccagccc acggacgata aagctttttg ctcccatcga accgggaata
     4321 attccatatt gaccagcacg cgcagacacc gcgcctttac gcgtcacgta gatctcttca
     4381 ccaaagtgct gttctttttg cacatagttg tggtgacagt tgatctcttc catcgccagc
     4441 gtttgtggct gtctgaccgt tttctgcgta atgctctgca acgccgttac cacgttttcc
     4501 atcatcgcat cgcggttaag gctggcgaaa agctgcgccc aggccacggc tcttaggtaa
     4561 tcatcaaagt attccgtacc ttccataaag tacgccaggt cgcgcgatgg caacgtttca
     4621 agctggtcct gcatctcttt ttgtgccaaa tcgataaagt aagtcccgat ggcgttacca
     4681 attccgcgtg aaccggagtg cagcataatc cacacctggt ctgactcatc aaggcagatt
     4741 tcaataaagt ggttacccgt tcccagcgtt cccaggtgtt tatagttgtt agtgttaagg
     4801 aaacgcggat atttttgcgt taaccactga taaccggctt caagctcagc ccatttcgca
     4861 tcgacgttaa caggcgggtt ttcccatgcg cctttatcac gtttacaacg gccagtggta
     4921 cgcccgtgcg gcacggccat ttcaatcgcc tgacgcagct ctgccaggtt ttcaggcagg
     4981 tcttccgccg ttaacgcggt acgcagcgcg ttcattccac agccaatatc cacgcccacc
     5041 gccgccggaa taatcgcccc tttggtcggg atcacgctac caatggtgga acctttaccc
     5101 aggtgtacat caggcattac cgcaatatgt ttgaaaataa acggcatctt cgccgtatta
     5161 ataagttgct gacgcgcatc ggcttctacc ggcacgcctt tggtccacat ttttaccggg
     5221 gcattttcag tggtcagtaa ttcgtaattc attttgtttt tctcttttcg ttgtttgctg
     5281 tcgtgataat tgcaaccgtt gtgccagaaa attaaataag cagattaatt ttttaattca
     5341 ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac aaaaccaatt
     5401 caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa cagtggcttt
     5461 tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcgaaatg
     5521 gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc tggaattgtt
     5581 gcacgacacc cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg ccagcgtttc
     5641 gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga
     5701 ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga
     5761 ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc
     5821 ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca aaaaagaaca
     5881 gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat
     5941 cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt ccggcattgc
     6001 cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgatcaaatc
     6061 ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc tggcgcgacg
     6121 catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg aagtgaactg
     6181 cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa aaggcgcgtt
     6241 taccggggcg cgggaatctc gtgaaggttt attacgcagc gccaacggcg gaatgttgtt
     6301 tcttgatgag attggcgaac tgggcgcaga cgaacaagca atgctgctaa aagccattga
     6361 agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat
     6421 cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc gcgaagatct
     6481 gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga
     6541 tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg gcgacagcgt
     6601 gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc cccaggcaac
     6661 atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac
     6721 tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc gctataactg
     6781 gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga
     6841 cctttttgac aggctgcaac tggaacacgt gatagctctc tgtcgccagg caaagtcgct
     6901 ttccgctgcc ggacgcctgc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga
     6961 cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga
     7021 tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc ttactggcgg
     7081 cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga ccatcgcgtt
     7141 acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct caataatggc
     7201 gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tatcgatgcc
     7261 gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc
     7321 gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa tgcggaaatc
     7381 ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac gcaaattgct
     7441 gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagtgt
     7501 cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt cttcggtctg
     7561 ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcaccgcc
     7621 atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc gaatagtctg
     7681 cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct gctgtttaac
     7741 cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt
     7801 ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaacccgg
     7861 cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc
     7921 agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt
     7981 caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc
     8041 acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg agagtaataa
     8101 caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac caccaccaga
     8161 gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc
     8221 agaactcaaa tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat
     8281 cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcacc acccaggtta
     8341 ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa tgcaggccac
     8401 tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc gggttttcga
     8461 gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc agccagacat
     8521 cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa
     8581 acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt
     8641 attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa
     8701 ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg
     8761 cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca ccatcaccgg
     8821 agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc
     8881 ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc
     8941 tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg
     9001 tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag
     9061 ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt acctaaagcg
     9121 cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag
     9181 ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc
     9241 gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct
     9301 tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac
     9361 tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct
     9421 ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg
     9481 tggatgattc ggattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg
     9541 ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat taagcgcgga
     9601 ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg
     9661 gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa
     9721 aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata tatctggcaa
     9781 gcgcgcggcc tggttaacgc caccggcccg tgggtgaaac agttcttcga cgacgggatg
     9841 catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc
     9901 gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg
     9961 atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggcgat
    10021 ccaaaagcgg tgaagattga agagagtgaa atcaactacc tgctgaaagt gtataacgcg
    10081 cactttaaaa agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg
    10141 ctgtgtgatg atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc
    10201 catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac
    10261 cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg cattggcccg
    10321 gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac
    10381 gccgctcgcc tgcgccgccg ttacccgttc ctgactgaat cgctggcgcg tcattacgca
    10441 cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat aagcgacctc
    10501 ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa
    10561 tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat gtggctgaat
    10621 gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg
    10681 ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga ttccttagac
    10741 tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac ttaagcacgt
    10801 tacccaattg ccccacttct tcctctggtt tctatttttt ctgcaccaat ggcaataata
    10861 tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc aaggctgcgg
    10921 ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat ttcggatatt
    10981 ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga gaattaacca
    11041 gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt caggaggcgt
    11101 tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg catattcaac
    11161 cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg gaaaggagaa
    11221 tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa taaccgtcgg
    11281 ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat aaagccggaa
    11341 tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc gcataaacac
    11401 tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc gggctgtttt
    11461 agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt atttctagcc
    11521 caggcattga tttgggtggt aatgacgtta tcgatatcgt cctggttatc tctgatataa
    11581 tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa gccgggaact
    11641 gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa catttccagc
    11701 atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata ctggcaggca
    11761 aatcgttgca aaattttcca ttcatcctca cgccaatttt ctttactggc aaggtttata
    11821 cgccgtagag aaaatacttc gtgaatattg tcaaactgcc cctgtgcaac aaattccagt
    11881 aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc tgggctacta
    11941 ttccatgcat tgattaagga atacgagatc gctcgtaacg atgtactccg taataccttt
    12001 tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg caccggataa
    12061 cgacgaaaaa tatgataaat ctgctcaatt aatgcctgca tggcggggct gggttgcata
    12121 attaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga gcattcgccg
    12181 cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg caactattgc
    12241 agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg tagagtttca
    12301 gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat ttccaggcat
    12361 cttttaagac ctgaaagata tagacatcct caatccagga gtcacatttt ttaagcgggc
    12421 gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt acggtgacgt
    12481 catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag gggtcaaatc
    12541 caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg gaaaatcccg
    12601 gccatacctt gctggaggtt aagccaatat ttaatgtgac attatatcga agcaatccct
    12661 caagaccgtc agcaccgacg aacttttccc cttcatccag ggcctggtta atgagttgct
    12721 gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata tccacgccag
    12781 tgaatggatc agtaccgaca aggcttgcaa ataatgtatt aactaattcc atttttatta
    12841 tccgccttaa tgcaacgttt tatacatcgg cacataacct acatcttcca ccaggctctg
    12901 cccctgcggc gttaaaaacc actccaccaa tttttgtgtt tctgacgtca tattttcccg
    12961 cgttaccata aaggcatcga cgatataggg atatttgccg ttgcgaatgt tttcggcagt
    13021 cggtgcaata ccgttaatcg ctagcagttt tatattttta tcggcgttca tttgtgttgc
    13081 gtaataacgg aaggtgtaac ctattgcgtt gtttgtatta cggtattcgg caacgacttt
    13141 aatcattccc tccatcatac ttgccacttc tgtttcctgc ggcgagatca tgcggacatt
    13201 tttcatgacc tgggattgca tcaccgtctg gctaccagag tcttccgggc gttgccaggt
    13261 ctggatctcc tgatcgttac cgccaaccgt gcgccagttg gtaattgcac cactgaagat
    13321 gtcacgcact tgttgttcgg tcagggaatt aaccgggtta tctacattga cgatgaaaac
    13381 aaacgcttca cgggcaaaag gcgtgtaaat caaagtgacg cccgattcct ccgcgcgttt
    13441 tttctgccca ccggaaggtt gagccacaaa gataatatcg gcattacctt taacaatctt
    13501 gttatatgct tcgggagtac gagagttcgc cagatattcc cattcgtgaa aatcttccgg
    13561 caaaacgctt agtgcataaa aggcagaggc ataaatgggg tacgccgccg ttgcgccatc
    13621 aagtcgcggc cagttttgcg tgaactgaat ttgcggttta ccacgcagtg gtgtcagttg
    13681 attattgagt ttatcgggtc gccaggccca ggtatcaata ttttcacgca caccgacgcc
    13741 gggattaagc tgtttatata aataagcctg gtaaccggcg acaaaaccaa gcatgagaag
    13801 gataaacaag agagaattac ggcaacgtaa cccggacgtg cccgttaaag gatatctggt
    13861 cggccagccg taccccagcg aaaaactgat atgcgcgcca acaggtataa tgactaacca
    13921 ccagtactca ccaccgacta atgaggctaa caaaacgccc agccagggga gcataagatc
    13981 agccaaccaa cccgctccca tctggcgaaa ttgataatct gccagatgca gcgcgattat
    14041 ccagaccccc agggttaata atgcgggtac taaaaaaaga cattgtcgcc agacggctat
    14101 ttctcttttt tctggtgcac tatcgtccgc ccacactcgc cccgagaaaa atagcattaa
    14161 tgcgcccagc gccagcataa aaatgttgag cagaaggtaa attgttggcg tgataaagat
    14221 attacaaact tcaataatac cggcaagtcc gacacccagc atggcaataa ccgccgccaa
    14281 aaactgcgcc agaatgggga tgccgaaaaa agtcattacc agcgaggtca aaatccattt
    14341 tctgttttgc attattcttt ccattctttc tgaatggcga aattatactc cccgagtccc
    14401 cttgcccctt ctggacactt ttccgaaatg atggcgaaaa aaacgggacc ctttggcccc
    14461 gttctattta ttagtgaaat tacaatctca ccggatcaat atgccagata tgatcggcgt
    14521 actctttgat agtacggtca gaagagaagt agcccatatt ggcaatgttc agcatcgctt
    14581 ttgcggtcca ctcttcctga agctcgtaga gttcgtcgac tttatcctga caatcgacat
    14641 agctgcgata atccgccaga acctgatagt gatcgccgaa gttgatcagc gaatcaacca
    14701 gatcgcgata gcgacccgga tcttccggac tgaatacacc gctgccgatt tgcgtcagca
    14761 cctgatgcag ctcctcatct ttctcgtagt attcacgcgg tttgtagccc tgacgacgca
    14821 gttcttccac ttcttccgca gtgttaccaa agataaagat attgtcagca ccgacatgat
    14881 ccagcatctc gacattcgca ccgtccaacg taccgatagt cagcgcaccg ttaagcgcaa
    14941 acttcatgtt actggtgccg gaagcttccg tccccgccag cgaaatctgt tcagacagat
    15001 cggccgccgg aatgatcaac tgcgccaggc taacgctgta gttcgggatg aacacgactt
    15061 tcagcttgtc gccaatctgc ggatcgttgt tgatcacttt cgctacgtca ttgatcaaat
    15121 gaataatgtg cttcgccatg taataggccg aagccgcctt accgccaaaa atattcacgc
    15181 gcggtaccca cttcgcatcc gggtcggcct tgatgcggtt atagcgggta atcacatgca
    15241 acacattcat caattgacgt ttgtattcgt gaatacgttt gatttgtaca tcgaacaacg
    15301 cctttggatt caccaccaca ttcagctgct gggcgatata ctctgccaga cgctttttgt
    15361 tctccagctt cgcctgatgc acagcgtgat taaccattgg gaaatcacag tgttgttgca
    15421 gctcattaag caggctaagg tcggtgcgcc agttacggcc caggtgttcg tccagcacgg
    15481 ctgaaagcga tgggttcgct accgctagcc agcgacgcgg cgtcacaccg ttggtgacgt
    15541 tggtgaaacg acccgggaag attttcgcaa agtcggcaaa caacgattgc accatcagat
    15601 tagagtgcag ttccgataca ccgttaactt tgtggctcac aacaaccgcc agccaggcca
    15661 tacgcacacg acgaccgttg gattcatcaa tgatcgacgc ccgtcccagc agatcggtat
    15721 cgttcggata ctgttcctgc aaggttttca ggaaatagtc gttgatttca aagatgatct
    15781 gcaggtgacg cggcagaatt ttacccagca tatcaaccgg ccaggtttcc agcgcctcgc
    15841 tcatcagcgt gtggttagtg taggagaaga cctgacaaca cacctcaaac gcgtcgtccc
    15901 agctaaattg gtgctcatcg atcagcagac gcatcatctc aggaatcgac agtaccggat
    15961 gggtatcatt gagatgaatc gcgattttat ccgccaggtt atcgtaggtt ttatgcaact
    16021 gataatggcg gcttaaaatg tcctgaatgg tcgaggaaac caggaagtat tcctgacgca
    16081 ggcgcagctc acgcccggag taggtggagt catccggata cagtacgcga gatacgttct
    16141 cggagtggtt tttatcttcc actgccgcga agtagtcacc ctggttgaat ttaccgaggt
    16201 taatttcgct actggcttgc gcactccaca aacgcaacgt gttggtcgcg tcggtatcgt
    16261 aaccagggat tatctgatcg taagcgactc ccagaatctc ttcggtttca atccagcgcg
    16321 tttttttacc ttcctgctga atgcgaccac caaaacggac tttatagcgc gtgttgtggc
    16381 gtttgaattc ccacgggtta ccgtattcca gccagtagtc tggcgactct ttctggctac
    16441 cgttaacgat gttctgcttg aacataccgt agtcatagcg gatgccgtaa ccgcgccccg
    16501 gcaaccctaa cgtcgccaga gaatcaagga agcaagccgc cagacgtccc aggccaccgt
    16561 taccgaggcc cggatcattt tcttcatcaa tcagctcttc gagatttaac cccatcgctt
    16621 ccagtgcgcc ctgtacatct tcgtaaattc ctagcgacaa catggcgttg gagagcgtac
    16681 ggccaatcaa aaactccatc gacaggtagt aaacctgacg agtttcttgc gacaactggg
    16741 cacggtttga acgtaaccag cgctccacga gacgatcgcg cacagcaaat aacgttgcgt
    16801 tcagccattc atgtttattg gcgacgaccg ggtcctttcc aatcgtaaac atcagcttgt
    16861 aagcgataga gtgcttaaga gcttctacgc taagcgtggg cgatgaatat gtaaacggag
    16921 cattcatata ggcgtttcct gaaaactatt tcaagcgata gtaaagctca cggtacgact
    16981 tcgccgcgac ctgccagcta aaatccattg ccatagcctg acgttgcaca aaccgccaca
    17041 gtgaaggacg ggaccacagt acaaaagcac gccgaatagc ccgtaacagc gaccaggcat
    17101 tactatcttc aaagacaaac ccactggcga cgccatctgc aaggttctca agagaacagt
    17161 cagaaaccgt atcagcaagc ccaccggtgc gccgcactaa cggcagcgta ccgtacttca
    17221 atccataaag ttgcgttaag ccgcacggtt caaaacggct gggcaccaga atgacgtccg
    17281 cgccgcccat aatgcgatgc gaaaatgctt cgtgatagcc aatctgaacg cccacctggc
    17341 cggggtattc cgctgccgcc gcaaggaaac cttcctgcag caccggatcg cccgcgccga
    17401 gtagcgccag ctgcccgccc tgctccagaa gacccggtaa ggcttccagc accaggtcga
    17461 gacctttctg gctggtcaga cggctcacca ctgcaaaaag cggcacttta tcgtcaacct
    17521 taagccccat tgcgatttgt aactggcgct tattttccgc tttatcttcc aacgtatcgc
    17581 gggtgtaacg cgaggccaac agtaagtccg tctctggact ccagattttc tcgtccacgc
    17641 cgttcagtac gccggaaaga cgcccttcac ggtgacgctg ttgcaacaga ccttccatac
    17701 cgtaggcaaa ctgcggttcg gtgatctcgc gagcgtaggt tggactgacc gccgtaatgt
    17761 gatcggcata gtacagaccg gccttcagga aagagatttg tccgttgaat tccagcccat
    17821 gaatattaaa gaatgaccat ggcaattgga tgtcattcat atgatgtgca taaaacatgc
    17881 cttgataggc caggttgtgc acagtaaaca ccgacttcgc cggacgcccg cgcgccgcca
    17941 gatacgcagg cgcaaggcct gcatgccagt cgtgcgcatg caccacatca ggacgccaga
    18001 atgggtcaag cccgctggcc atttctgccc caacccaccc cagcagcgca aaacgcaata
    18061 cgttgtcggt ataggcaaat aagttggtat cgtgatacgg gcttcccgga cgatcataga
    18121 gatgcggcgc gtcaatcagg tatatgccaa ccccgttgta atgaccgaac aacagcgtga
    18181 tatgtccggc gaaggtatca cgacgggata ctacctgcgc atcggtaacg ccacggcgaa
    18241 tatcgggaaa tgcaggcaac agtacgcgag cgtcaacgcc gtctgcgatt tgtgctgcgg
    18301 gtaatgcccc aataacatca gccagaccgc cggttttaag cagcgggaac atctctgaac
    18361 atacatgtaa aacctgcatt atcgctcctg tttatgccct aacttccgta gcatttcgcg
    18421 cgttaccagc acgatgcctt cttctgaacg atagaaacga cgtgcatctt cctctgcgtt
    18481 ttcaccaatc accatgcctt ccggaataac acaagcacga tcgatgacgc agcggcgcag
    18541 acggcacgag cgacctaccc atacttccgg taacaatacg gcggaatcaa tgttgcagaa
    18601 tgaattcacg cgaacgcgcg agaacagaac ggactgcacc accaccgaac cggagatcac
    18661 acagccgccg gataccagtg agttaagggt catcccgtgg ctaccggagc gatcctgcac
    18721 gaatttcgct ggcggtaatg attcattgta ggtgcgaatt ggccaattgc gatcgtacat
    18781 atccagttcc ggcaccacag aggccagatc gaggttcgct ttccagtaag cttccagcgt
    18841 acccacatcg cgccagtacg gctcggcatc cgggtcggat tgtacgcaag agagcgggaa
    18901 cgggtgcgca taggccagac cggcttcggt gatcttggga atcaaatctt tgccaaagtc
    18961 gtggctggag ttctcatcac gatcgtcttc ttccagcagt tcatacagat agtcggcgtc
    19021 aaagacgtag atacccatac tcgccagaga tttgctcgga tcgttcggca ttgacggtgg
    19081 gttagcaggt ttttcaacga attcgataat tttatcgttc tcatcaaccg ccataacgcc
    19141 aaatgcggag gcttcttcaa tcggtactgg catacaagca acggtgcaac gtgcgccttt
    19201 ttcgacgtga tcgataagca tacgcgagta gtcttgcttg tagatatggt cgcccgccag
    19261 gatcaccacg tattccgctt tataacggcg gataatgtcg aggttttggg tgaccgcatc
    19321 tgcggtgccg cgataccagt tttccccttt cattctctgc tgtgctggca gcagatcgac
    19381 aaactcgttc atttcttcat tgaagaatga ccagccgcgc tgaatgtgct gcaccagagt
    19441 gtgggactgg tactgggtga tcacgcccat acgacggatc ccggagttga tgcagttaga
    19501 cagcgcaaag tcgataatgc ggaacttacc gccgaagtgt acggccggtt ttgctcgctt
    19561 attggttaaa tccttcaggc gggtaccacg tcctcccgcc agtatcaggg caacagattt
    19621 caatggcagc tggcgcgcca acattaagtg atcgttcttc tctaaactaa ccatgactaa
    19681 ctcctttttt atcatctctg gaacacacac aatccgtgtg caggtccctg ccagacagcc
    19741 gtaatcactg ggttatcctc tccagcgaat gggggaatgg cgtgccactc cccagcaggt
    19801 aaaacaatct ctgttacctc aagcgtggcg ttaattgcga tcaaaaagcg atccgagagc
    19861 agaatttgca gctgtttcgg cccgttttgc cactcatccg tgcttaaagg ttgagcatat
    19921 cgatttagcc aacggacatt gccgtcgcct tcttcccacc agcgattctc caccaaagcg
    19981 ggaatgcgct tgcgcagatg gattaacgcg gcggtaaatg cggttaaacc actgcttgcc
    20041 tgcgaccagt ccaaccaggt taattggtta tcctgacagt aggcattgtt attgccatgc
    20101 tggctgtgac catgttcgtc accggccagt aacatcggcg taccctggga gagcaacaac
    20161 gttgttaaca gggcgtgaat gctgtcgcgc cgccgttcaa ccaggtcaag agaaccgcct
    20221 aacccttctt taccatgatt gttactgtaa ttgttgttgg tcccgtcgcg attttcttct
    20281 ccgtttgctt cattgtgttt atggttgaag caaacgcagt cgcgaagcgt aaaaccgtca
    20341 tgcgcggtga cgagattaat cgcggcactc ggcagacgac cattacgttt aaaaacatcg
    20401 ctggaggcag caaaacgccc ggcaaacgcc cccagaggca aatcataatg tagccagaaa
    20461 cgacgggcag catcgcggaa atgatcgttc cactcggcaa acagcggcgg gaaatttccc
    20521 acctgataac caccaggagc gatatcccac ggttcagcaa ttaacttcac ctgcgagagc
    20581 accgggcagt tctggatagc ggtaaacaac ggcgcatcct gacggaactc tggcgtacgg
    20641 cccatgactg ccgccagatc aaagcggaaa ccatcgacgt ggcaggtttc tacccaataa
    20701 cgcaggcagg cgctggcata atccaccacc gccggatgac tcaaattgag cgtgttgccg
    20761 caaccggtcc agttgtgata atcgccgtct tctcttatcc aataatagct acggttatcg
    20821 atcccacgca gcgagaataa cgggccgtcg aggtccagtt ccgcactatg gttgagcacg
    20881 atatcaagaa tgacttcgat acccgcttta tgcagtgctt tgattgcatc gcgaaactca
    20941 tccagcgccg tttctggcga gcaggcatac gccggatgca gcgcaaacat cgccaccggg
    21001 ttgtaacccc agtagttact tagccccatg cgttgcagac gtggttcact ggcaaactgc
    21061 gccactggca gcagttccag cgcggtaatg cccaattgtt tcaaatagtt gatcatcacc
    21121 ggatgcccga gggctttata agtgccacgg atctcgaccg ggatctccgg gtgcaagtac
    21181 gttaatcctt tgacatgggc ttcataaatg atggtgctgc cccacggcgt gcgcggcggg
    21241 gcatcatctt cccagtcata gtgatcaacc accactacgc atttcggcgc aatggcggcg
    21301 ttgtcgcgat agtcaggttc attatgaccg gcgtgcagca gcgggttatc tttaaactcc
    21361 ccctcaattt gccgcgcgca aggatcaatc aacaacttcg ccgggttaaa gcgatgcccc
    21421 tcggcgggtt gccaggggcc atgaacgcga taaccataac gcaaacccgg gcgcgcatcc
    21481 ggcagataac cgtgccaaat gtcgccactg tgccctggca agtcatagcg atgttcctgg
    21541 ccattggcgt caaagacaca cagttctacc cgctcggcat gagcggagaa aagtgtgaag
    21601 ttgacgccct gaccgtcgta atgcgcgccg aggggagcgg gtttgccaat ggcgagttgt
    21661 gtcattctgc ctcccgaacc agccagatag tggccagcgg tggtagcgtc aggcttagtg
    21721 aatgctgacg accgtggctg gcaatctcat cgctgtgtac cgtgccgcca ttgcctgcat
    21781 tactgccgtg atagtgcatg gaatcggtat tgaggatttc acgccatttg cccggctggt
    21841 ttatgccgaa gcgataatca tgacgcggca ccggcgtaaa gttactggca acgatgattt
    21901 cgttaccctc tttatcgcga cgcacaaaga tcagcaccga gcgttctttg tcatccacca
    21961 ccagccattc aaagccgtac gggtcaaaat ccagttcatg cattgcttta tggtggcggt
    22021 aggtgaggtt cagatcgcgc accagacgct ggacaccgtg gtgccagtta tcgccgcctt
    22081 ccaacagatg ccagtcgagg ctggcgtcat ggttccactc gcggccctgg gcaaattcgt
    22141 tacccatgaa cagtagtttc ttgcccggga atgcccacat ccagccatag taggcgcgca
    22201 ggttcgcgaa tttctgccat gcgtcgcccg gcatgcggtc gagaatcgat tttttaccgt
    22261 ggaccacttc atcatgcgac aacggcagga cgaagttttc agtgtagttg tagagaatcc
    22321 cgaaggtcag tttatcgtga tgatactgac gataaaccgg gtcgagcttc atgtagtcca
    22381 gggtgtcatg catccagccg aggttccact tgtaccagaa gcccagaccg cccatatcct
    22441 gcggacgaga aacgccaggg aaatcggtag actcctcagc cattgtcacc gcaccggaaa
    22501 cctgctcacc aagaatacgg ttggtattac gcaagaattc aatcgcttca agattctcgc
    22561 gcccgccaaa ttcgttcggg atccactccc cctctttacg gctgtagtcg cgataaatca
    22621 ttgacgccac cgcatcgacg cgcagcgcat caataccaaa acgttcaatc cagtaaagcg
    22681 cgttaccgac gaggaagtta ctgacttcac ggcgaccata gttgtagatc agcgtgttcc
    22741 agtcctgatg atagccttca cgcggatcgc tgtgttcata caagttcgtg ccatcaaatt
    22801 cggcaagcgc aaagtcatca gtcgggaagt ggcctggcac ccagtcgaga atcacgttca
    22861 gaccagctgc gtgtgcggca tcaatgaaat aacggaagtc gtcgcgagta ccaaaacggc
    22921 gggttggtgc atacaggccg gttggctgat aaccccaact gccatcgaag ggatgctcgt
    22981 taatgggcag tagttcgagg tgggtaaagc ccatccattt agcataaggc accagttgat
    23041 cggccagctc gcggtagctc aaccagaaat tgttgtcggt gtggcgacgc caggaaccca
    23101 ggtgaacttc ataaatagag attggcgcat caaactgatt cgcttttttg cgctcttcag
    23161 tctgtacaac cttttccggc agcccgcaaa taagagacgc ggtttccggg cgcatttgcg
    23221 cttcgaaggc ataagggtcg gacttcagac gcaagttgcc attggcatca atcatctcgt
    23281 atttatagag ctgaccgtta tgcgccccag ggataaacag ttcccagatg ccgctctctt
    23341 tacgcaggcg catcgggtga cggcgaccgt cccagtagtt gaattgccca accaccgaga
    23401 cccgacgggc gtttggagcc cagacagaga aacgcgtacc tgtgacgcca tccatagtat
    23461 ctgcatgcgc gcctaaggtt tcatacgggc gcaggtgagt accttcagat aatagccagg
    23521 catccatttc ctggattagc ggaccaaaac ggtaaggatc atcaatcagg ttttgctgac
    23581 catgccagac aacagccaac tgatagcgga aaaaattctt acgtcgcgga atgacgccgc
    23641 taaagaatcc ccgtgagtcg agacactcca gttttgcgag tttgcgcccg gttttcggtt
    23701 caatcaccca cacatcggta gcgtcgggta aaagggcacg gacttccagt cccgcggtgg
    23761 ttttatgcat tcccagtacg gaaaaaggat ccgcaaaatg gcctgcaatt agcgcgttaa
    23821 tcacgtctct atcgatacga tcggacatgc ttgtcttcct gttttattgt gtcaccccat
    23881 ccaattggat ttttgacttc tggttgtgac atttttttga cctgaacggc gcagcactct
    23941 gtgcatcctc tctgcgtcgt cctcacttca ggtaaggctg tgaatactca tgtattcagc
    24001 cacccttaaa gaatagccaa tgctctattt aactcccggt aaatcatgaa acatctgcgc
    24061 ttactcctgt attacgcact aacaggggcg gcatcgcgcc ccagatttaa tgaataaaga
    24121 ttacgccagt tgacgaagca tccgacgcag cggctccgcg gccccccaca gcagctggtc
    24181 gcccacggta aaggctgaca ggaactctgg tcccatattc agcttacgca ggcggcctac
    24241 cggcgtggtc agcgtgccgg taacggcagc tggggttagc tcacgcatag tgatttcccg
    24301 atcgttcgga acgactttcg cccacggatt gtgcgcagcc agcagttctt ccacggtcgg
    24361 aatagacaca tcttttttca atttaatagt gaatgcctgg ctgtggcagc gcaatgcccc
    24421 gacacgcaca cataaaccat ctaccggaat tacggaagat gtgttgagga tcttgttggt
    24481 ttccgcctgc cctttccact cttcgcggct ctgaccgtta tcgagctgtt tgtcgatcca
    24541 cggaatcagg ctacccgcca gcggcacgcc aaagttatcc accggcagct caccgctacg
    24601 ggttaaggtt gtgactttgc gttcgatatc gagaatagca gaggacgggg tcgcgagttc
    24661 atctgccaca tggccataca gatggcccat ctgggttaat aactcacgca tatgtcgcgc
    24721 accaccgccg gaagcggcct ggtaggttgc aacggacacc caatcaacaa gatcattggc
    24781 gaataaacca cccaacgaca tcaacatcag gcttacggta cagttaccgc caacaaaagt
    24841 cctgatgcca ttatttaatc cgtcggtaat gacgtcctga ttgacggggt caagaatgat
    24901 gatggcgtca tctttcatgc gcagagacga tgctgcgtca atccagtaac cttgccatcc
    24961 gctttcacga agctttggat agatttcgtt ggtataatcg ccgccctgac aggtcacaat
    25021 gatatcgagg gcctttagcg cctccagatc aaaggcatcc tgaagtgtgc cagtggttcc
    25081 gccaaaagac ggcgcagcct ggccaagctg agaagtagaa aagaagacag ggcgaatggc
    25141 gtcgaagtcg cgctcttcaa ccatgcgttg catgagaacg gagccgacca taccgcgcca
    25201 gccgataaaa ccaacatttt tcataagcgt ttttttcctg caaagatgtg tgctgtataa
    25261 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt
    25321 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    25381 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    25441 gttttattga tcctgattat ggatccgctc ggaaacctac ctattttcat gtccgtactg
    25501 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    25561 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    25621 gcagaaaccg tctccatttc tggcggcatc attctgtttc taattgccat taagatgatt
    25681 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    25741 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    25801 catcagtacc cgaatcagat ggggcatctg gtgattgctc tgctgctggc ctggggcggc
    25861 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    25921 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc
    25981 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gaacccggta
    26041 gtgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    26101 atggcaccga cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    26161 tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac gtggctgaca
    26221 acaatcgaac caccagcgat acaaatcgac agcgccgcca tttgcgcacc ggagtagttc
    26281 agttgttcaa taaccggcat caccagtcct accgccgtta aacaggctac ggtggcagaa
    26341 ccctgaatga tgcgcactgc agctgccagc acgaagcagg tgatagcaat cggcaggccc
    26401 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    26461 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg
    26521 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    26581 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    26641 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    26701 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttgccttc gccgagatgc
    26761 ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc ggctgatgaa attaccccac
    26821 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    26881 ccaaaatcgg cgttcatctg cgatgcgagg agcattggcg ctggcccagg caccaggaac
    26941 gcagccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    27001 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    27061 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    27121 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg taagatcttg
    27181 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    27241 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    27301 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    27361 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    27421 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    27481 accagtacat cagtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    27541 atttgggttt taaagaaatg gcctttgcgc gctttcaggc ggctttcaat cacgtcaaaa
    27601 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    27661 tgttttttca atgcagaaca gacgatcagt gacactttat tagtgcgctg catagcaaac
    27721 gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt cgtcattcag cggttcgcca
    27781 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    27841 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcctga gacgcccatc
    27901 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    27961 aattaaaaat gttacgggta actgttatcg gtaacattgt ccggccggac aatatcagaa
    28021 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa
    28081 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    28141 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    28201 agcacgctcg ccagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca
    28261 atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc
    28321 gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    28381 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatacggc
    28441 accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    28501 tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc gcgcaataat ggcggtagtc
    28561 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    28621 ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    28681 gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggacagcat ggattcgaga
    28741 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatcgtctg ataaccgtgc
    28801 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    28861 ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg
    28921 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    28981 tgctccgggt tacgcaaaaa gcggctgacc gtcattttgg tcacgcctac acggtcagcc
    29041 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    29101 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    29161 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    29221 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    29281 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    29341 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    29401 cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc gttcagcggc
    29461 aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg
    29521 atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac
    29581 ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca
    29641 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    29701 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    29761 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    29821 gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    29881 aaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc aggagtccag
    29941 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    30001 ccccatgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    30061 gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca aacttactta
    30121 gcgagagtta ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattc
    30181 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    30241 gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcacg
    30301 ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg
    30361 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    30421 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    30481 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    30541 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    30601 ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg cggcagccca
    30661 ggtttggtct ctgccaccgg gcggtaatag tcaaaatggc tttccacttc aacaatctct
    30721 cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc aaagcgacga
    30781 ttctgatacg gcgtgacggt cagccctttg cttttcgcca acgcaaacag ctccttcgcc
    30841 tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc ttccagcgcg
    30901 cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag cttaacatcg
    30961 ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga tatgggaata aatgggagcc
    31021 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    31081 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    31141 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    31201 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    31261 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    31321 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat aataatcatt
    31381 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    31441 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    31501 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    31561 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    31621 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    31681 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    31741 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    31801 gtgcgacaac tggttgcggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcccc
    31861 ggcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta agaagtacgc
    31921 attgcgtaat ggtgaatatg tcgatgcata ttatatggcg cgggtgaagt aagatagtgc
    31981 cctttttctg agatggaaaa agggtgtcat tcaaaatcga cataccttcc tttaaggtat
    32041 ttatttgccc aatacataaa ttgagattta act
//
LOCUS       DAFAVF010000040        31789 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFAVF010000040 DAFAVF010000000
VERSION     DAFAVF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.040"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(121..208)
                     /locus_tag="LGK42_001814"
     tRNA            complement(121..208)
                     /locus_tag="LGK42_001814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Ser,seq:tga)
     gene            635..1753
                     /gene="hyaA"
                     /locus_tag="LGK42_001815"
     CDS             635..1753
                     /gene="hyaA"
                     /locus_tag="LGK42_001815"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBK2658071.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1750..3543
                     /gene="hyaB"
                     /locus_tag="LGK42_001816"
     CDS             1750..3543
                     /gene="hyaB"
                     /locus_tag="LGK42_001816"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBK2658072.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3562..4269
                     /gene="hyaC"
                     /locus_tag="LGK42_001817"
     CDS             3562..4269
                     /gene="hyaC"
                     /locus_tag="LGK42_001817"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBK2658073.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4266..4853
                     /locus_tag="LGK42_001818"
     CDS             4266..4853
                     /locus_tag="LGK42_001818"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBK2658074.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            4850..5248
                     /gene="hyaE"
                     /locus_tag="LGK42_001819"
     CDS             4850..5248
                     /gene="hyaE"
                     /locus_tag="LGK42_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBK2658075.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVESQQERAS"
     gene            5245..6102
                     /locus_tag="LGK42_001820"
     CDS             5245..6102
                     /locus_tag="LGK42_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBK2658076.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6236..7780
                     /gene="appC"
                     /locus_tag="LGK42_001821"
     CDS             6236..7780
                     /gene="appC"
                     /locus_tag="LGK42_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBK2658077.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            7792..8928
                     /gene="appB"
                     /locus_tag="LGK42_001822"
     CDS             7792..8928
                     /gene="appB"
                     /locus_tag="LGK42_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBK2658078.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            8941..9033
                     /gene="cbdX"
                     /locus_tag="LGK42_001823"
     CDS             8941..9033
                     /gene="cbdX"
                     /locus_tag="LGK42_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBK2658079.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9113..10411
                     /gene="appA"
                     /locus_tag="LGK42_001824"
     CDS             9113..10411
                     /gene="appA"
                     /locus_tag="LGK42_001824"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBK2658080.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10526..12706)
                     /gene="etk"
                     /locus_tag="LGK42_001825"
     CDS             complement(10526..12706)
                     /gene="etk"
                     /locus_tag="LGK42_001825"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBK2658081.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(12726..13172)
                     /gene="etp"
                     /locus_tag="LGK42_001826"
                     /gene_synonym="yccY"
     CDS             complement(12726..13172)
                     /gene="etp"
                     /locus_tag="LGK42_001826"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBK2658082.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13160..14299)
                     /locus_tag="LGK42_001827"
     CDS             complement(13160..14299)
                     /locus_tag="LGK42_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBK2658083.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14345..16441)
                     /locus_tag="LGK42_001828"
     CDS             complement(14345..16441)
                     /locus_tag="LGK42_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBK2658084.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16441..17187)
                     /locus_tag="LGK42_001829"
     CDS             complement(16441..17187)
                     /locus_tag="LGK42_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBK2658085.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17184..17828)
                     /gene="gfcB"
                     /locus_tag="LGK42_001830"
     CDS             complement(17184..17828)
                     /gene="gfcB"
                     /locus_tag="LGK42_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBK2658086.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(17935..18240)
                     /locus_tag="LGK42_001831"
     CDS             complement(17935..18240)
                     /locus_tag="LGK42_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HBK2658087.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(18682..18894)
                     /gene="cspH"
                     /locus_tag="LGK42_001832"
     CDS             complement(18682..18894)
                     /gene="cspH"
                     /locus_tag="LGK42_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBK2658088.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19180..19392
                     /gene="cspG"
                     /locus_tag="LGK42_001833"
     CDS             19180..19392
                     /gene="cspG"
                     /locus_tag="LGK42_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBK2658089.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19403..19591
                     /locus_tag="LGK42_001834"
     CDS             19403..19591
                     /locus_tag="LGK42_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK2658090.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            19566..19796
                     /gene="ymcE"
                     /locus_tag="LGK42_001835"
     CDS             19566..19796
                     /gene="ymcE"
                     /locus_tag="LGK42_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBK2658091.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            19786..19959
                     /locus_tag="LGK42_001836"
     CDS             19786..19959
                     /locus_tag="LGK42_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBK2658092.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20007..21080)
                     /locus_tag="LGK42_001837"
     CDS             complement(20007..21080)
                     /locus_tag="LGK42_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK2658093.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21152..23896)
                     /gene="torS"
                     /locus_tag="LGK42_001838"
     CDS             complement(21152..23896)
                     /gene="torS"
                     /locus_tag="LGK42_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBK2658094.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            23979..25007
                     /gene="torT"
                     /locus_tag="LGK42_001839"
     CDS             23979..25007
                     /gene="torT"
                     /locus_tag="LGK42_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBK2658095.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(24980..25672)
                     /gene="torR"
                     /locus_tag="LGK42_001840"
     CDS             complement(24980..25672)
                     /gene="torR"
                     /locus_tag="LGK42_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBK2658096.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            25802..26974
                     /gene="torC"
                     /locus_tag="LGK42_001841"
     CDS             25802..26974
                     /gene="torC"
                     /locus_tag="LGK42_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBK2658097.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            26974..29520
                     /gene="torA"
                     /locus_tag="LGK42_001842"
     CDS             26974..29520
                     /gene="torA"
                     /locus_tag="LGK42_001842"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBK2658098.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            29517..30116
                     /gene="torD"
                     /locus_tag="LGK42_001843"
     CDS             29517..30116
                     /gene="torD"
                     /locus_tag="LGK42_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBK2658099.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNALENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(30209..30514)
                     /gene="cbpM"
                     /locus_tag="LGK42_001844"
     CDS             complement(30209..30514)
                     /gene="cbpM"
                     /locus_tag="LGK42_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBK2658100.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(30514..31434)
                     /gene="cbpA"
                     /locus_tag="LGK42_001845"
     CDS             complement(30514..31434)
                     /gene="cbpA"
                     /locus_tag="LGK42_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBK2658101.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         7511 a         8153 c         8382 g         7743 t
ORIGIN      
        1 catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc
       61 tgagaatttc gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt
      121 tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg
      181 gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg
      241 gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg
      301 tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt actcgtttag
      361 caaccggcta aacatcccca ccgcccggcc aaaagaaaaa taggtccatt tttatcgcta
      421 aaagataaat ccacacagtt tgtattgttt tgtgcaaaag tttcactacg ctttattaac
      481 aatactttct ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc agcatctcac
      541 gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc cgaccataag cgcccggtgt
      601 cctgccggtg tcgcaaggag aagagacgtg cgatatgaat aacgaggaaa cattttacca
      661 ggccatgcgg cgtcagggcg ttacccggcg cagctttctc aaatattgta gtctggctgc
      721 cacgtcgctg ggattaggcg cgggaatggc accaaagatt gcctgggcgc tggagaacaa
      781 accccgcatt ccggtggtat ggatccacgg tctggaatgc acctgctgta ccgaatcttt
      841 tatccgctcc gctcacccac tggcgaagga cgtcatcctt tccctgattt ccctcgatta
      901 tgacgatact ttgatggctg ccgccggaac ccaggcggaa gaagtcttcg aagacatcat
      961 cacgcaatac aatggcaaat atatcctcgc agtagaaggt aatccgccgc tgggcgagca
     1021 ggggatgttc tgtatcagca gcggtcgacc gtttattgag aaactcaaac gtgccgctgc
     1081 cggagccagc gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc
     1141 gcggcccaat ccgacgcagg caacgcctat cgacaaagtc atcaccgaca aacccattat
     1201 caaagtacct ggctgcccgc cgatcccgga tgtgatgagc gccatcatta cttacatggt
     1261 gacctttgat cgcttgccag atgtcgacag aatgggccgt ccgctgatgt tctatggtca
     1321 gcgaatccac gataaatgct atcgccgcgc ccacttcgac gccggagagt tcgtccagag
     1381 ttgggatgat gacgctgccc gcaaaggtta ctgcctgtac aaaatgggct gcaaagggcc
     1441 taccacctat aacgcctgtt cctccacacg ctggaatgat ggcgtttctt tcccaatcca
     1501 gtctggtcac ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta
     1561 cagccgcgtg gtcgatattc cgcaaatggg tactcattcc accgccgata ccgtcggttt
     1621 aaccgcgctt ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca gcgccgttga
     1681 ccagcgcaga cgtcataacc agcaacctac agaaaccgaa catcagccag gcaatgagga
     1741 taaacaggca tgagcactca gtacgaaact cagggataca ccatcaataa tgccggacgc
     1801 cgcctggtgg tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga agtgaatatt
     1861 aacgatcaga atgtgatcac caatgccgtc tcctgcggca ccatgtttcg cgggctggag
     1921 atcattctgc aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc
     1981 gtctgtactg gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc tatcggtatt
     2041 aaagtgccgg acaacgccaa tatcatccgc aacattatgc tggcaacgct ctggtgccac
     2101 gatcatctgg tgcacttcta tcagcttgcc gggatggact ggatcgatgt gttagatgcg
     2161 ctgaaagccg acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa
     2221 tcatcccctg gctatttctt cgacgtacaa aaccgcctga aaaaatttgt tgaaggcggg
     2281 cagttgggga tcttccgcaa tggctactgg gggcacccgc agtacaaact gccgccagaa
     2341 gctaacctga tgggctttgc ccactatctc gaagctctcg atttccagcg tgaaattgtg
     2401 aaaatccacg cggtctttgg cggtaaaaac ccgcatccaa actggattgt cggcgggatg
     2461 ccttgcgcca ttaacattga cgaaagcggc gcggtcgggg cggtcaatat ggaacgcctg
     2521 aacctggtgc agtcgattat cacccgcacg gcagacttca ttaacaacgt gatgatcccc
     2581 gacgccttag ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac gggtctttct
     2641 gataaatgtg tcctcagcta cggcgcattc ccggatattg ccaacgactt tggtgagaaa
     2701 agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt gctgccagtg
     2761 gatttggttg atccgcagca ggtgcaggag tttgtcgatc acgcctggta tcgttatccc
     2821 aacgatcagg tcgggcgtca tccattcgat ggcattaccg acccgtggta caaccccggc
     2881 gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg ctactcgtgg
     2941 atcaaagcgc cacgctggcg cggtaacgcg atggaagtgg ggccgctggc acgcacgtta
     3001 atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg
     3061 ttgaacctgc cgctttccgg tatccagtca acgttaggcc gcattttgtg ccgcgcgcac
     3121 gaagcgcagt gggccgcagg taagttgcag tatttcttcg acaagctgat gaccaacctg
     3181 aaaaacggca atctcgccac tgcttccacg gaaaaatggg aacctgcaac ctggccgaca
     3241 gagtgccgtg gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca ctgggccgcc
     3301 attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg gaacgccagc
     3361 ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa caccaaaatg
     3421 gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga cccgtgcctc
     3481 gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc tgatctccgt gcaggtgcgt
     3541 taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag ccactatgtc
     3601 tttgaagcgc cagtccgcat ctggcactgg ttgacggtgt tatgcatggc ggtgttgatg
     3661 gtcaccggat actttatcgg caagccgcta ccttccgtca gcggcgaggc gacgtatctg
     3721 ttctatatgg gctacatcag gttaattcac ttcagcgccg ggatggtttt taccgtggtt
     3781 ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat actcccgcga gctgtttatc
     3841 gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg
     3901 tttctggcaa aacgtccgag tgccgatata ggccataatc ccatcgccca ggcggcgatg
     3961 ttcggctatt tcctgatgtc ggtctttatg atcatcactg gttttgcgct gtacagcgaa
     4021 cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt ctactggacg
     4081 ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct gattggcgcg
     4141 tttgtgatcg gtcatgtcta catggcactg cgtgaagaca tcatgtccga cgacacggtg
     4201 atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag taacaaggag
     4261 cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg tgggccgatg
     4321 aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg cccgagtatg
     4381 tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc gaaagcgcca
     4441 gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg ctgcgaacct
     4501 atgccggaga acgcattccg gcttatctca gcgcgaagaa aatgagcctg catcagaaca
     4561 gtttctccga agtgttggcg ctggcggata tccgcggaca tctgccagca catattgccc
     4621 tcgtcggtct gcaacccgca atgctcgacg actacggcgg tagcctgagc gaactggcac
     4681 gggagcaact gcccgctgcg gaacaggcgg cgctggcgca gctggctgcg tggggaattg
     4741 tgccgcaacc ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg atggaaaatt
     4801 acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga tgagcaacga
     4861 cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc cagtcagtga
     4921 atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat taagcagtga
     4981 cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat tactgcgcga
     5041 gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg aagccatcgg
     5101 cgatcgcttt ggcgtctttc gctttcctgc cactttagtg tttaccggcg gaaactatcg
     5161 cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc gcgggcttgt
     5221 cgaatcgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct ggggccagga
     5281 acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg tcaggtgaat
     5341 gatgaaccga gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt gattgcgctg
     5401 ttaaacgagt tacaacatca actaagcgaa cgccaaccgc cgttgggcga ggtgctggca
     5461 gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct tctcggggaa
     5521 ggggaagtgt cagtgcgcat tcagcaggct gacgacagtg aaagtgaaat acaggaggcg
     5581 atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa
     5641 ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg
     5701 acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc gttggcgcat
     5761 gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa tctgacgcaa
     5821 ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc gggaaatatt
     5881 cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt acgccatgtc
     5941 tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta tgaaatctgc
     6001 ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc gcagcggctt
     6061 agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc
     6121 tttcagtctt attaatatcg caatcggcga atacctctgg tcgtagagtt tcaggataaa
     6181 gagggagatc taccattatc gggttatttt tctctcttcg cctacaggag tgcgcatgtg
     6241 ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc actttttatt
     6301 tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca tttacgtggt
     6361 caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct tcggtatcaa
     6421 ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta ctaactggtc
     6481 attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg aagcattaat
     6541 ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc agcggctgaa
     6601 taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc tctctgcatt
     6661 gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt ttgatatcga
     6721 caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg tcagccaggt
     6781 gaaatttgtg cacaccgtaa tggcgggcta cgtgaccggg gccatgttta ttatggcgat
     6841 cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgtt cgtttgccat
     6901 cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag acagttctgc
     6961 gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg agtggcaaac
     7021 ggaacctgca cctgcaccgt tccatgtggt tgcctggccg gaacaggatc aagagcgtaa
     7081 cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact cattagataa
     7141 acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc aacgcggacg
     7201 tatggcctgg ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc atgtgttgca
     7261 ggcattccgg gaactggaag gtgacctggg ctacggcatg ttgctctccc gctatgcgcc
     7321 ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg cgatacctca
     7381 ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc tgctgctact
     7441 ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc atcgctgggt
     7501 gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag ccgggtggtt
     7561 tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga catactccgc
     7621 gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg tagggcttta
     7681 caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc tggggccgag
     7741 cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat catgtttgat
     7801 tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt ggtctttatg
     7861 atctccgacg gatttgacat ggggatcggc tgtctgctgc cgctggtggc gcgtaatgat
     7921 gatgaacgcc ggatagtgat aaacagcgtt ggtgcacact gggaaggcaa ccaggtctgg
     7981 ttgatcctcg ctggtggggc attatttgcc gcctggccca gagtgtatgc agcggcgttt
     8041 tccggctttt atgtggcgat gatcctggtg ctgtgctcac tgttcttccg cccgctggcc
     8101 tttgattatc gcggaaaaat cgccgatgca cgctggcgta aaatgtggga cgccggtctg
     8161 gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa cttgttgctc
     8221 ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atcttggcag cttctggcaa
     8281 ctgctgacgc cattcccttt attgtgcgga ttgctcagcc ttgggatggt gattttgcaa
     8341 ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc acagctggcc
     8401 accaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta ctggctgtgg
     8461 gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc caatccgtta
     8521 atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga atcgcccgtt
     8581 ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat ggcgatttat
     8641 cgtggtcgcc cgggttgggg atttttaatg gcgtcattga tgcaatttgg cgtgattttc
     8701 acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc aatctccagc
     8761 ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt ggtaatcgtg
     8821 ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa aatgtgggga
     8881 cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg agcaggaaca
     8941 atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc cacccttgtg
     9001 ttggtatggc tggacccgcg tctgaaaagt taacgaacgt aggcctgatg cggcgcatta
     9061 gcatcgcatc aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat cgatgaaagc
     9121 gatcttaatc ccatttttat ctcttctgat tccgttaacc ccgcaatctg cattcgctca
     9181 gagtgagccg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg gtgtgcgtgc
     9241 tccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc caacctggcc
     9301 ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcctatc tcggacatta
     9361 ccaacgccag cgtctggtag ccgacggatt gctggcgaaa aagggctgcc cgcagtctgg
     9421 tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg aagccttcgc
     9481 cgccgggctg gcacctgact gtgcaataac cgtacatacc caggcagata cgtccagtcc
     9541 cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg cgaacgtgac
     9601 tgacgcgatc ctcagcaggg caggagggtc aattgctgac tttaccgggc atcggcaaac
     9661 ggcgtttcgc gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt gccttaaacg
     9721 tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac tcaaggtgag
     9781 cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga cggagatatt
     9841 tctcctgcaa caagcacagg gaatgccgga gccggggtgg ggaaggatca ccgattcaca
     9901 ccagtggaac accttgctaa gtttgcataa cgcgcaattt tatttgctac aacgcacgcc
     9961 agaggttgcc cgcagccgcg ccaccccgtt attggatttg atcatggcag cgttgacgcc
    10021 ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtgc tgtttatcgc
    10081 cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact ggacgcttcc
    10141 cggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct ggcgtcggct
    10201 aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac agcagatgcg
    10261 tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga ccctggcagg
    10321 atgtgaagag cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc aaatcgtgaa
    10381 tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc agttacctga
    10441 atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga aaaggcgttc
    10501 acgccgcatc cggccacttt cagttttact ctttctcgga gtaactataa ccgtaatagt
    10561 tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg atagcgcctt
    10621 taatattgac gcctgcctgt tccagacgct gcattgacaa actcacctct ttggcggtgt
    10681 tcaagccaaa acgcgcaacc agcaggctgg tgccaacaga acgccccacg accgcggcat
    10741 cactcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg tcgttcgccc
    10801 attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta ggtggcacct
    10861 gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact ttgttgagct
    10921 catctttacc tgccagatat tccgacaagc catgttcatt actcacggta aacaggttat
    10981 gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg gactgggcga
    11041 tcaccgctgc cagagttgaa ctgacaaacg ttttaccact gtctggcgtc gcaccggtga
    11101 tcatcagaat gttattctcc gtctccatca tagcgaaatg cagactggtt cgtagcgcac
    11161 gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg atatttttag
    11221 tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggtg cgtttatcca
    11281 gccactcgga cattgggata gtggcataaa cgctgatgcc gtgctcttcc agttgttccg
    11341 gggcttctac accacgacgc aacatcgcac gcgccagcac ggcacccaca gaaataaaca
    11401 ggccaagaat aaaaccaagc accacattca acgctttttt cggtttcact ggctgcggct
    11461 gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc gaaatactca
    11521 actcctgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct acgtcacgac
    11581 ttaaacgcaa cacttcctgt tgggtggaag gcattgccga tacccgctta ttcaggcgtt
    11641 tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt gggtgatctt
    11701 tcttatacag ctgggagatc tctgcctcgc ggaaggtcag ctcattgagt tgattatcaa
    11761 cgttcacaat ctgctcaaga acggctttgg cttccaggtt aaggtcaacc gaatcgcgct
    11821 gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc acttcaggta
    11881 actggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga gcgatattct
    11941 gttgcaaata gttgttagcg atgctgttca gaatacgagt aatcaactgg ggatcatcac
    12001 cagtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg gtaaaggttt
    12061 cctgcaatgc gttaatcgct tccagttcgg tacgctggct caggacaaac tgtgttcctg
    12121 gtttggcctt aatgtccgcg atagtcagcg caacgccatc tttttccaga cgctggccga
    12181 ccataccatt gacggtgaac tcttcacctt ccagtgtata gtggccgttt tccccaaccg
    12241 tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag ctgatcgcca
    12301 gctcacctgg tttttctttg gttaatctcg cccagccgcg acccacaatc ggaaaatact
    12361 tctgctcaac tatgtcgcgc agattcagtt cagcaatggt tttaccgaga atcatgcgcg
    12421 attgcagcag ttggatctcc ggtgcagact cgggcgatga gttagggatc atatcgctca
    12481 ggccgctgag aatggcgttg ccctgttttt gctcaacctg gaccagagta tctgcctgat
    12541 aaattggtgt acttaacagc gagtaagcga cagcgatcag cgtgaataac gcggtcacgc
    12601 tgataataaa cttacggtga tcccataact cgccgaccag acgaagcaga tcgatctcat
    12661 tttcctgagt gctgcctggt ggcgtattca tatttttagt tgtcatcgta ttttataaac
    12721 tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa cataccgtag
    12781 acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt ctgttccagc
    12841 cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt aacctgagca
    12901 atatgctctg actccattgc cagaatcaga tcgtaatttc gtgccatctc agcagtgagc
    12961 ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc tgctgctgtc
    13021 gcatctgcag ggtgttttac cagaccatga acacctgccg atttgacttt tacgcctggc
    13081 agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc ggtacaaacc
    13141 accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa gtctctgtca
    13201 ggtcatgcac cccggaaatc gtcggtacca gcagagaaat cacacggttc caacgagcca
    13261 gcggcgcagt agtgacataa acaatgtcgt aaggttcaag ctggaattcc gttccgagta
    13321 tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg ccattctgct
    13381 tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc tgattcatac
    13441 cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt ttcagcgtgc
    13501 tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc ggaataaata
    13561 aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc gccacgctgc atcagcgcat
    13621 agaggttcac tttggtcttt acgccattct gggtgagcac cacgttgcgc cagtctgcat
    13681 ctgcggtcag accgcccgcg gcgttaatcg catccatgat ggtcagagga atattggtaa
    13741 tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc gagcggaacg
    13801 ccgcgacgct gacatcgact tgcgggcttt caatgactga atccagacgc gctgtaatct
    13861 cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata taggggtaga
    13921 aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg tattgccctg
    13981 cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatcc ccaatgccga
    14041 tacgatattc atagtttgcg atttgctgct ccagctccgg gttagcctgc gacataatcg
    14101 gtttggcgcg caactgatca atcaatcctg gcgtgacggg atagatattg accattttgt
    14161 ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc gataaattct
    14221 gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgtcagt accgaaaact
    14281 taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta tgaattacgt
    14341 caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc tggtatttgc
    14401 gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc gcgcggttgc
    14461 ggttaggtcc aatggtcatc aagtcgaatg gaatcgagat ataaaaacct ttactaaagc
    14521 cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc cctaccgcca
    14581 cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcgcc tttatctttt gccagatatt
    14641 gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag taagccgtca
    14701 cgaaacctgt tggcgtggaa taatcggtga aacgcatcat gttgtcccag tcacgctgct
    14761 tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga taaagcagct
    14821 cggaaccgac accggcatac atcgtttcca gataaccgcc atacacctgg ccatagaaac
    14881 cattgcctaa gtcagcaaaa tagttcgcct gcaagttgtt gagataaacg tcattgcgaa
    14941 cgtagtcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggc aacagcgaag
    15001 acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat accgccatca agcagcaggt
    15061 ggtcggtaaa ccagtagcgg gcactggaca tcagccccag ctggaacata tagaaatctt
    15121 ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga gtagctaaag cgatcttcac
    15181 gaatacggta gccccgacca aaggcagaaa gatcttctgc ttcaacacgt tgttgttgaa
    15241 gttgctctga ttgaccaggc gctgtaccag ccagctgttt gcgcaggctg gctacgtcgg
    15301 tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc ttctcaacgc
    15361 cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttcg cgagaatcac
    15421 ggtatttgta ttgttgacca gacatataca gcgtcttatc gcgcagctga atttccggtg
    15481 cttcaaaacc tgcgttgtac ttcagcgcgg taagttggtt cgctactgtg gtgtactgca
    15541 atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc agggcagggc
    15601 gcagatcgtt gaaattggtc cgtaacgtga agccaaacat caacgtgtta ccgcgttcat
    15661 aactcaggtt gagatctgcc cagctggcag cgcgataaac tgcgccgacg ttgaaatggc
    15721 ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gttgccatcg tattcgagtt
    15781 tcagacgcag gggattccac ggcgtttgat actcaatgcc gccaaagatg gaagccgggc
    15841 cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg cgatgacaat
    15901 atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg gcgtagcccc
    15961 atgccattcc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc agatactcac
    16021 cgtcaaacag acccgtacca gcaatatcac gtttaccaaa cgccacttgc ggtagccagt
    16081 aaccttcttc ccacaggcga agtttaaaat cgaatgattt gtctttgtat gactgatcgc
    16141 cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac atccgtataa cgaatggtgc
    16201 cttccagcca tgggaacagc gccacggagg tggagtagaa ccggtattga tcgttatccc
    16261 ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc tgcaacaatc
    16321 ctgtgccgcc aaagtctgat tgcgacgaac ccagcggatc cgggtaggtt aatacttcag
    16381 catgacaggc actggagacg gcaatggcca ggcagcttaa aagataagaa ttcttcttca
    16441 tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat cagcatattt
    16501 ttcgggcagt acatgcgcag aaaagcccaa ccaaagctgg ctgccaggcg gtggttccac
    16561 atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag gcgtaatcac
    16621 cataacgtta ttcttgtccg cgcctgcaag gcgggggtga tcttgcagat aatcggtaac
    16681 gctacggcct gcctgccagg gcaattgccc agccccagaa accgcaccca gcagagtaat
    16741 agtcaccgga cgctgtacgg tatagagcgt atagtccccg accaacgggg gattgctgtt
    16801 ctcatccact cgaacaaaat cagggtccag tttgaccggt aagcgaccag tgatattgag
    16861 attgagtaac tgctggcgca cggatttaat cgttgccgcc acatcgtcgt cagcttctgc
    16921 ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt tagctttcgc
    16981 cgcagagtcg gtcagcaagg caccaggcca ccagaggcga tcgcgcagtt gcggttgtgt
    17041 caccaactgg acaacatttt ccacggggcc aacggaaagt gtctgttgtt cgccaggcag
    17101 ataaatagtc accgttcctt gcgcaaaggc atggggtgtc attacgtaaa gtacgctggc
    17161 aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat ttctacggga
    17221 ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc cgaatcgacc
    17281 cagaatgtgt tttgccactg tttttctggc cgcgtggaag tcacctcttc ctgccagaca
    17341 ttacacagca cattgcgacc cgcaatattc agcgtctcta atccagcaaa ggaaaaagta
    17401 gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agagaatatc gcgcgtccag
    17461 cggctgccat cttttattgc cagcgcgtta cgcagagggt cctgcccgga atttgtcact
    17521 tccagcagat tattattaag tttgacggtt tttaaaagac gtccattgtg ggtaaccagc
    17581 atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc cagtaccaca
    17641 aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa cgcctggatc
    17701 tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa tgtatcgacc
    17761 atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga taaaataaga
    17821 gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatctcta aagcccggta
    17881 cgaatatcgc aaccgggtat catttaactt taatttcgac gcgttaaagc agtattagcg
    17941 tgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt catttgctgc
    18001 aacaactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg tcgccgtgcc
    18061 gacagcgcca gcagccacgc caccagtggt agtagcagtc gtggttgcgc cggtagtggt
    18121 cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgttgctt caggggcagc
    18181 aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct tgtgtttcat
    18241 aacttttcct ttattcatcg catggacaat acgtacgctt gagatcagga ttgtctactt
    18301 tgagtataac atccttgtga aatctccctt tcctcatacc gcttgcacta tatactgcgg
    18361 gtaggaaagg cgcgcagagg gaaatataag attgttaact atccccctct ggaaatacga
    18421 cttgtgagca taagataaat cttagcaaaa tacaaattaa agataaggaa attgttacga
    18481 aagctattaa ttattgttgg taatatagtt tcaagtggta ctataactgg ctgttaaatg
    18541 gcatttataa ctattaggtg cagagatatt cgcttaaatg gattagtttc tttgtggaaa
    18601 agtaactgat gttataatgt atgatgttga ctattgcact attccaataa aaccagtcag
    18661 ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg gtcctcgcag
    18721 gccatttacg cggcaaaatt ccacacgtaa tcctggtata agcacttctg cgtcgcgggg
    18781 agtgaatgcg gaaatatgga cctgaacttc tttacgaccg tcggagggga taatgaatcc
    18841 tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg acaaacaaat
    18901 tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc cagcgatatt
    18961 taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc
    19021 tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc
    19081 aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag
    19141 ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt
    19201 ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc
    19261 aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa
    19321 aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt
    19381 gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca
    19441 tggttcacag taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa
    19501 gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac
    19561 tgaccatgtg ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta
    19621 tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg
    19681 aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca
    19741 ccttactggc gtgttgctgt ctttcaagac cagaagaggt taacagtgaa tattgaagag
    19801 ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg
    19861 aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc
    19921 gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcacccgtgc
    19981 gaggtgatgt tccgcttgtt gctaatttag tgaccaatca ttggcgcttg tggaattaag
    20041 cgtcggtaca attcatccgg taccgggctt tgccatactc ccgcatacat tgcgtaacca
    20101 atcaccgcaa acataatccc cagaaccagt agcgtcatta accagccaga caacgcaaag
    20161 gcttttttat ttgccgcagg tttttgcagt gaaaaggtca atgttgaggc taccggacat
    20221 gactctacgc aagtcataca gccggtacat tccactgttc gtacctgaat taatttatcg
    20281 accgggatcc gtgatgggca attttttgcg catttgccac agtcgataca actttcggca
    20341 ttgcgacgaa tcttaaacgg cgacaatagc gaaaccacgc ccatcagcgc gccatatggg
    20401 caaagataac gacaccaggc atggcgaata aacaggctgg caatcagcaa aacggtcacg
    20461 ctgattaatg tcgcggtccc catatgacga aagaaatcga gcattttaac gtccatcacc
    20521 acgctgtagg gcgacaacat aaaatagtga atcgcctgag cgggcatcaa taacgcgata
    20581 tagagaaaaa aactcaacag caaatacttc acgccgcgca gaggaatatc cagccagcgg
    20641 ggaaggacac attgccgacc aaacagtttg ttaccgagat cgccgattaa ttcagaaagc
    20701 gtaccaaccg ggcataacca tgagcaaaag gcctttttga gtaatagact gatgacgata
    20761 aaagcgacca ataacagcat cgcggcggcg tggacggacg gtaactgacc tgttacaagg
    20821 ctatatttca gattcatcag cccggcaatc ggtagccagc cttcgatacc tcccggtctg
    20881 gcgacaaatg tcgtgctact tgccgtttcg taatagcgca cccaatacca gaacgtgatg
    20941 gcaatataaa tattcgttgc caacagtaat aattgcgtcg ctttacgcca ggtcgtggca
    21001 ttacgccagt cattccacgg taatttgccg cccgtcgtgc ctggccgccg ctgccagcgg
    21061 gttcttttat tctctgccat gattttgcca gtccgttaag ttgtatacca aatgccacta
    21121 ttctagttgt tcttaactgg ctgatattga ttcaaatcgc gttcaggtct ttcttatgca
    21181 accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg ggggcagata
    21241 atggctgctg ctccagttgt gcgcatagct ggctggcgat acgcattccc agactcgagc
    21301 aactgctttt tagctgatgc gcggcacgct ttattttctc gctatcctga ctggcgcggg
    21361 caatgtcgat ttcatcgaga agcggcaggg catgttgtgt aaataatacc agccattcgt
    21421 ggatcttctc cgtccccatt aactgagcat cttcattgag ttgcgataca tccagcgatt
    21481 gatcgttatt gacttgcagt tggagatagt gcgccagtaa ctgaccgagc acttcacgcg
    21541 gcaccggttt agggataatc ccgcggaata atgaactggt acgctggcgc agcgtttcgt
    21601 caatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt gccagttgtc
    21661 gggcaagcgt aatgccgtcg acatccggca gatcaaaatc caccagtgcg gcagcaaacg
    21721 gttcgctatt ttgcagtgtc tctaaagcct gcgcggcatt gccaacagca acaacctgcg
    21781 caccactggt gttcagcatc tcaacggtaa ttcgctgggt tagcgggtta tcttcaatta
    21841 acagcaaacg taaaccgtca agacgcaccg cctgattgac tgtttttggc acgggtgccg
    21901 tggcaacacg taacggcaag cgtaaacaaa aacagcttcc aacctccggc gtgctggtgg
    21961 cgctcagttc gccgcccatc gcctgggcca gacggctact gatagtcagc cccagcccgg
    22021 tgccgccgcg tttgccgctt acctgcacaa atggctggaa gatttctgcc agtttcgcgg
    22081 gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgctcgcca tcagtgcgac
    22141 tacgcaggat aatgtaccct tcgtcagtaa aacgcagggc gttgctcaac aggttggtta
    22201 taacctgacg aatacgtcgt ggatcgccca ttaacgcgca cggcatatca tcggcaattg
    22261 ccgttgccag gcgaatcggg cgacctttca cccgtccgct cattaattgc agggtacttt
    22321 ccagcagcgg gcgcggttca aagggctcat cgctgaccga aacattcttg ccacctgctt
    22381 cgatagcgga ataatcgaga atatcgttga ggatggtcag caacgattca ccagagtcag
    22441 taattgcccg caaatcatca cgctgggcgt taagtgcggg gttatctgcc agcagttgag
    22501 cagtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc gccagaaacg
    22561 ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt tctatcacca
    22621 gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg cggtggcgat
    22681 tcagtgcatg aacattgctg cgaaacgcat ccatcagccg cccgatggta tccagctccc
    22741 gtacgccagc ggtttccggg aaaggggagt caatatcacc gtccagcagc cgttgcagcg
    22801 cctgcgtttg ttcggcaagt ggacgcgtga ctgagcgata aaccacgcgc caaaggatca
    22861 gaatcagtgc gcaaagtgaa accatcccca gcaataacag gctgtattgc ccgcgtgcac
    22921 tggctttttc cagatgtgcc agtccatgct gattgcgcag ctcaatggtg tcgaccagct
    22981 gactgacttc gctactaaac tgcgcgaact gggcgatatt attttgggcg agagtttgca
    23041 ggtgattgct gatttcactg tcctgctgga acagcgccaa cagatcgcta tattggctaa
    23101 cggtagttaa cgttgttgcg acctgcgcac gaacacccgg atcttcaatg cgtatttgcc
    23161 gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc gcatttttct
    23221 ggatctgctc cagccccaga ttcatcacca tttgctgcac ccgcagggcg ctaaggcgca
    23281 gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg agtgcacttt
    23341 ctgcggcctg acgctgatct tgttcgatca aatcgtaaat cccggcctgg gtcgctccgg
    23401 cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatctca tcggcggcag
    23461 cgactatctg ctgactgagt tgctgttgtt gctggcgcag ttgcaaacgc tgccccacca
    23521 gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca atagcggtgg
    23581 tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg ctttgtgcgg
    23641 tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc aggttctgcg
    23701 cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc atggtaggaa
    23761 tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg taccatccca
    23821 ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag agtcttcggg
    23881 tcagggttaa attcacggtc ggtgcacttt aggtgaaaaa ggttgagtcg caaagcggaa
    23941 tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg ctatttttac
    24001 ttctttccct tttcatgttg ccggcatttt cggctgataa cctgttgcgc tggcatgatg
    24061 cgcagcattt cacggtgcaa gcctctacgc cgcttaaagc caaacgcgca tggaaactgt
    24121 gcgcgcttta tcccagcctg aaagattcat attggttatc gttgaactat ggtatgcagg
    24181 aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc tacagccagt
    24241 tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag gccattttgc
    24301 tcggtagtag cacgacctca tttcccgacc tgcaaaagca ggtagcaagt ctgccggtga
    24361 tcgaactggt aaatgctatt gatgctcccc aggtgaaaag ccgcgttggt gtgccctggt
    24421 ttcagatggg ctatcaaccg gggcgatatc tggtgcaatg ggcgcacggt aaaccactga
    24481 atgtgctgtt gatgcccgga cccgataacg ccgggggcag taaggagatg gtcgagggtt
    24541 ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt ggtgataacg
    24601 atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccaccca gaaatcgacg
    24661 tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt aacctgaaaa
    24721 cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg ctgaagcggg
    24781 gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg gcagttgagc
    24841 aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca ccgattttag
    24901 ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca ccagggggat
    24961 ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct tcaccatgtt
    25021 gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac acatcgacgg
    25081 tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgtagcaga cgttcacggc
    25141 tgagaatttc gcccggattc gtcacaaatg ccaccaacat ttcatactct gcgcgggtca
    25201 gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg caataaccgg
    25261 caaagcgata gcagttgtcc tgagtgtgcg gttgagcttg tcgcgcgagg tcgattcgcc
    25321 agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg gtgacgtaat
    25381 cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt ccggtaacca
    25441 gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg ccattttcat
    25501 cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata atttcccgca
    25561 gcccggcacc gctcgctgta acggaaacgg tatacccctc ctgagtgaag taagattgta
    25621 atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc atcagagggt
    25681 tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc ctgaccagtg
    25741 ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataatttta caggggctat
    25801 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
    25861 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
    25921 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
    25981 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
    26041 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaatgatat
    26101 ctaccagacc tttattgctc actccattga tacacctgaa aaattcgaag ccaaacgcgc
    26161 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgccgctc
    26221 ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg cacgtcagat
    26281 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
    26341 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
    26401 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
    26461 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
    26521 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
    26581 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
    26641 ggtaaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
    26701 ggccactgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggctta
    26761 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
    26821 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
    26881 taaacgtgaa gaacgcacct tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
    26941 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
    27001 tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat gctggggccg
    27061 tcattgttaa cgccgcgccg tgcgactgcg gcgcaagcgg cgactgacgc tgtcatctcg
    27121 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt gaaggatggt
    27181 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gattgccgga
    27241 ttgccggatc acgtacacaa cgcggcgcgt attcgttatc cgatggtacg cgtggactgg
    27301 ctgcgtaagc gccatctcag cgatacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
    27361 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtgca gaaaactcac
    27421 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
    27481 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
    27541 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat ggaagtgtat
    27601 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
    27661 tctgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
    27721 tattacgcgc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat cagcatcgat
    27781 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
    27841 aacccgcaaa ctgatgtgcc gctgcaactg gcgctggcgt atacgctgta cagtgaaaac
    27901 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtac
    27961 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
    28021 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
    28081 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
    28141 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggctttgg ttttggctgg
    28201 cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg tttctccggc
    28261 tctacgtcga ttccgcctgt tcacgacaac agtgattaca aaggctacag cagcaccatt
    28321 ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt
    28381 aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ccggaactaa cccattccat
    28441 cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac ggttatcgcc
    28501 atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc tgcgaccacg
    28561 cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaaccgtgg cattatcgcc
    28621 atgaaacagg tggtgccgcc gcagttcgag gcgcgcaacg actttgatat tttccgcgag
    28681 ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga aatgggctgg
    28741 ctgaaacgca tctggcagga aggtgtacag caaggcaaag gacgcggcgt tcatctgcca
    28801 gcgtttgatg acttctggaa taacaaagag tacgtcgagt ttgaccatcc gcagatgttt
    28861 gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac gccgagtggc
    28921 ctgattgaga tctactcgaa aaccatcgcc gatatgaact acgacgattg tcaggggcat
    28981 ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ccggctcgca gaagtatccg
    29041 ttgcatctgc aatctgtgca tccggatttt cgacttcact cgcagttatg tgagtcggaa
    29101 actctgcgtc agcaatatac ggtagcgggt aaagagccag tattcattaa cccgcaggat
    29161 gccagcgcgc gcggtattcg taacggtgat gtggtacgcg tctttaacgc tcgcggtcag
    29221 gtgttggcag gggcagtggt ttctgaccgc tatgcacccg gtgtggcacg aattcacgaa
    29281 ggggcatggt acgatccaga taaaggcggc gagcctggtg cgctgtgcaa atacggtaac
    29341 cccaacgtgt tgaccatcga catcggtaca tcgcagctgg cgcaggcgac cagtgcgcac
    29401 actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac ggcgtttaac
    29461 ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg cgtcgcaggt gaaatcatga
    29521 ccacgctgac agcacaacag attgcctgta tttacgcctg gctggcgcag ttgttctccc
    29581 gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct gaatggtttt
    29641 cgttgctgaa aagcgaaccg ccgctggctg cggcggtgaa tgcgctggaa aaccgtattg
    29701 ccgctctgac agtacgtgac gatgcccgtc tggaactggc cgctgacttt tgcggcctgt
    29761 ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa caggacgagc
    29821 aagagattaa acgcttgtta gttgaggcag ggatggaaac cagcggcaat ttcaacgaac
    29881 cggcagatca tctggcgatc tatctcgagt tgttgagtca tctgcatttt tcgctgggag
    29941 aggggaccgt tcctgcgcga agaatcgaca gtttgcggca aaaaacactg acggcgctgt
    30001 ggcaatggtt accagagttt gttgcgcgtt gccatcagca tgacagcttt ggtttttacg
    30061 cggcactaag ccagttattg ctggtgttag tggagtgcga ccaccaaaac agataacgtc
    30121 gtttgtgcgc ctgaaaagac gcgtttagcg tcgcatcagg cattatgggg cagttgccgg
    30181 atgcgtcatc acatccggca aacacacatc acggatgagt tacaaaccgg gaaagtcgct
    30241 ggcgcagcag gcggttttcc tgcttcaggt gcgcaatatc atccattaac gtcagcgcca
    30301 ccgcgatccc aggccagtcc agagccagtt catgacgcag gcgtactgcg cgttgcacca
    30361 cgatggcggc atggtcgtca aatacccagg ttgtttcctg gatctcacgc ggttcaacca
    30421 cccccaaacc gacaatttca ttcaactcct cttcagagat gccggtatgc aggcaaaatt
    30481 cggtaatagt aaaagtcacc gtaacattag ccattatgct ttcccccaat ctttacgtgg
    30541 atcaaaagac gactgggcgt ctgccagttg ctgccacagc gcggcagtgt tttcatccgg
    30601 tttcggcggc atcacgattt tcagtaccgc atacagatcg ccggtctgtt ttttgctcac
    30661 cagacctttg cctttaacgc gcaatcgttg cccggcctgg ctgcctggcg ggatagtcag
    30721 caaaatgctt tctttcagtg ttggaacggt gactttagca cccagcgccg cttcccacgg
    30781 gctaaccggc actacaattt ccagatcatg gccgacaata tcgaacagcg gatgtggcgc
    30841 aatatgaatc accagccaca aatcgccatt tggaccgccg ttttcgcccg gcgtcccctg
    30901 gcctttcaga cggatgcgtt gaccattgcc gacgcccgcc gggatcttga cattcagcgt
    30961 tttcggaatt tcctgttcga tcatgccaaa ggcgttataa accggcaggt tatagctgat
    31021 ggtacgctta tgctcagtca gcgtttcttc gaggaatacc gccacttcga tttcaatatc
    31081 gtggccgcgt gtggcggggc gttgacggct ctggcgggca tgctgaccga aaattgacga
    31141 gaagatatcg tcaaaatctt cggcgttaaa actctgaccg tcgctatgat ggaactgacg
    31201 gttaaattgc ggatcgttgc gatgttgcca catctgatca tactcagcgc gacgttgttc
    31261 atcacttaac acttcccagg cttcagcgac ctctttgaag cgggcttcgg catccggttc
    31321 tttgctgaca tcaggatggt atttgcgggc cagtcgacga taggcggtct tgattgtctt
    31381 gagatcgtcc gtcggtttca cgcccatgat ggcgtaataa tcctttaatt ccatagcgtt
    31441 atctcgcgta aatcaacaca aattgaagga acccctgtaa ggtaactcct ataagtgtag
    31501 ggtaatcctc aaaatttcat atgccaacac agaatatgtt attgaaatca tcgcggagag
    31561 gaggtcgcct caaggtgggg tgctgaacat attttaaaca ggtgaaaaag ggtgagcgat
    31621 ttttgatagt tgaaccaggc actttaagtt taactggggc gtcattattt attaaatttt
    31681 atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt
    31741 gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaaca
//
LOCUS       DAFAVF010000041        31734 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFAVF010000041 DAFAVF010000000
VERSION     DAFAVF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.041"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(22..909)
                     /gene="rarD"
                     /locus_tag="LGK42_001846"
     CDS             complement(22..909)
                     /gene="rarD"
                     /locus_tag="LGK42_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBK2658102.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(961..1428)
                     /locus_tag="LGK42_001847"
     CDS             complement(961..1428)
                     /locus_tag="LGK42_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBK2658103.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1593..2462
                     /gene="pldA"
                     /locus_tag="LGK42_001848"
     CDS             1593..2462
                     /gene="pldA"
                     /locus_tag="LGK42_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBK2658104.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2595..4424
                     /gene="recQ"
                     /locus_tag="LGK42_001849"
     CDS             2595..4424
                     /gene="recQ"
                     /locus_tag="LGK42_001849"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK2658105.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4488..5108
                     /gene="rhtC"
                     /locus_tag="LGK42_001850"
     CDS             4488..5108
                     /gene="rhtC"
                     /locus_tag="LGK42_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBK2658106.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5170..5790)
                     /gene="rhtB"
                     /locus_tag="LGK42_001851"
     CDS             complement(5170..5790)
                     /gene="rhtB"
                     /locus_tag="LGK42_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBK2658107.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5901..6923
                     /gene="pldB"
                     /locus_tag="LGK42_001852"
     CDS             5901..6923
                     /gene="pldB"
                     /locus_tag="LGK42_001852"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBK2658108.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6931..7731
                     /gene="yigL"
                     /locus_tag="LGK42_001853"
     CDS             6931..7731
                     /gene="yigL"
                     /locus_tag="LGK42_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBK2658109.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7807..8706
                     /gene="bioP"
                     /locus_tag="LGK42_001854"
     CDS             7807..8706
                     /gene="bioP"
                     /locus_tag="LGK42_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBK2658110.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8594..9547)
                     /gene="metR"
                     /locus_tag="LGK42_001855"
     CDS             complement(8594..9547)
                     /gene="metR"
                     /locus_tag="LGK42_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBK2658111.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9665..11926
                     /gene="metE"
                     /locus_tag="LGK42_001856"
     CDS             9665..11926
                     /gene="metE"
                     /locus_tag="LGK42_001856"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK2658112.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11965..12780)
                     /locus_tag="LGK42_001857"
     CDS             complement(11965..12780)
                     /locus_tag="LGK42_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBK2658113.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13042..13803
                     /gene="udp"
                     /locus_tag="LGK42_001858"
     CDS             13042..13803
                     /gene="udp"
                     /locus_tag="LGK42_001858"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBK2658114.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13944..15371
                     /gene="rmuC"
                     /locus_tag="LGK42_001859"
     CDS             13944..15371
                     /gene="rmuC"
                     /locus_tag="LGK42_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBK2658115.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15466..16221
                     /gene="ubiE"
                     /locus_tag="LGK42_001860"
     CDS             15466..16221
                     /gene="ubiE"
                     /locus_tag="LGK42_001860"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBK2658116.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16235..16840
                     /gene="ubiJ"
                     /locus_tag="LGK42_001861"
     CDS             16235..16840
                     /gene="ubiJ"
                     /locus_tag="LGK42_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBK2658117.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16837..18477
                     /gene="ubiB"
                     /locus_tag="LGK42_001862"
     CDS             16837..18477
                     /gene="ubiB"
                     /locus_tag="LGK42_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBK2658118.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18556..18825
                     /gene="tatA"
                     /locus_tag="LGK42_001863"
     CDS             18556..18825
                     /gene="tatA"
                     /locus_tag="LGK42_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBK2658119.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQANTNQEQAKTEDAKRHDKEQV"
     gene            18829..19344
                     /gene="tatB"
                     /locus_tag="LGK42_001864"
     CDS             18829..19344
                     /gene="tatB"
                     /locus_tag="LGK42_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBK2658120.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19347..20123
                     /gene="tatC"
                     /locus_tag="LGK42_001865"
     CDS             19347..20123
                     /gene="tatC"
                     /locus_tag="LGK42_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBK2658121.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20165..20947
                     /gene="tatD"
                     /locus_tag="LGK42_001866"
     CDS             20165..20947
                     /gene="tatD"
                     /locus_tag="LGK42_001866"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBK2658122.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20944..21432)
                     /gene="rfaH"
                     /locus_tag="LGK42_001867"
     CDS             complement(20944..21432)
                     /gene="rfaH"
                     /locus_tag="LGK42_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBK2658123.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLVPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21599..23092
                     /gene="ubiD"
                     /locus_tag="LGK42_001868"
     CDS             21599..23092
                     /gene="ubiD"
                     /locus_tag="LGK42_001868"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBK2658124.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23138..23839
                     /gene="fre"
                     /locus_tag="LGK42_001869"
     CDS             23138..23839
                     /gene="fre"
                     /locus_tag="LGK42_001869"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBK2658125.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24031..25194)
                     /gene="fadA"
                     /locus_tag="LGK42_001870"
     CDS             complement(24031..25194)
                     /gene="fadA"
                     /locus_tag="LGK42_001870"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBK2658126.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25204..27393)
                     /gene="fadB"
                     /locus_tag="LGK42_001871"
     CDS             complement(25204..27393)
                     /gene="fadB"
                     /locus_tag="LGK42_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBK2658127.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27583..28914
                     /gene="pepQ"
                     /locus_tag="LGK42_001872"
     CDS             27583..28914
                     /gene="pepQ"
                     /locus_tag="LGK42_001872"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBK2658128.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28914..29528
                     /locus_tag="LGK42_001873"
     CDS             28914..29528
                     /locus_tag="LGK42_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBK2658129.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29567..31018
                     /gene="trkH"
                     /locus_tag="LGK42_001874"
     CDS             29567..31018
                     /gene="trkH"
                     /locus_tag="LGK42_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBK2658130.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31030..31575
                     /gene="hemG"
                     /locus_tag="LGK42_001875"
     CDS             31030..31575
                     /gene="hemG"
                     /locus_tag="LGK42_001875"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBK2658131.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7353 a         8123 c         8698 g         7560 t
ORIGIN      
        1 acggcctacc catgttaatt cttaactttt tctacgctgc gtataaatcg catccatcac
       61 aaaaattgcc agcgccaccc aaataaaggc gaaagtcacc atcttatcgg cacccggttt
      121 ttcaccataa aacgtcacag ccagcaggaa catcagcgtc gggccaatgt actggaaaaa
      181 gcctaacgtt gagagacgca agcgcgtggc agcggcggta aaacacaaca gcggtacggt
      241 agtgacaata ccggcggcga tcagcagtaa attcagcgac atcgggtttt gccccatatg
      301 gctggttgag ctgtcggcaa tagcaaacag gtaaattgcc gccacgggca gcagccacat
      361 ggtttcgatt aacatgccgg tttgcgcttc aacggcaatc ttcttgcgta ccagaccgta
      421 gaaggcaaaa ctaaatgcca gtcccagcgc gataataggt agcgaaccaa aagtccacag
      481 ctggactaac acgccacata tcgccagaat caccgccagc cattgcatcc ggcggaatcg
      541 ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc gggttaataa agtaaccaag
      601 gctcgcttcc agcatatggt gattgttcac cgcccagata aacagtagcc agttgccacc
      661 aatcagcacg gcagagactg ccagcataaa aattttctgt ggcgtctgaa tcagcgtttt
      721 taaataggac cactggcggc aaatgctcat cagcaccacc ataaagaaaa acgaccagat
      781 cacgcgatgc gtcaggattt catcggcagg cacgtagtaa atcaacttga agtacgctgg
      841 cgctataccc caaataaaat aagcggcaag agcgagtaat acgccctgcc gcgtttgttt
      901 tgcatccatc gggaatactc atttttaatt ggtaacagca gtttacctgc ttttatgtct
      961 tcaacctacc atataggtgg cggtggcact ggcaatataa agctgttctt cattgtgtaa
     1021 ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac aggctactgg tagcagtaaa
     1081 acgctcgccc ctgcctgggc gcagataatc aacgcgaaga tcaatggtcc ccatccgcga
     1141 tagccgctgg cgtagttcat cttcactgat ggtttcgtgg cgggttaagg tacttcccac
     1201 gcacaccaga ccggcggcga catccagcgc cgacgcaatg accccgccgt gcaaaatgct
     1261 ttgcgcccag ttgcccacca tcattggctg atttttaaag gccagctgtg cgaactcttt
     1321 ttcgtaacgc tccagttcca tccccaatgc gcggttaaat ggcatgtgat aaacaaacat
     1381 ctcacccact aatttcaggg cttgttcagc ggtcagtacg gcagacatat catccttaca
     1441 cttcattagt taatgaaatg ttgattttat gcttctttgt tgttggtttc tactttagga
     1501 agggataact aacggctatg gagttaagta tgtaaaatag cccgcagaaa aatattcacc
     1561 ttatcaataa ttcgttacgg agaacacgac cgatgcggac tctgcagggc tggttgttgc
     1621 cggtgtttat gttgcctatg gcagtatatg cacaagaggc aacggtgaaa gaggtgcatg
     1681 acgcgccagc ggtgcgtggc agtattatcg ccaatatgct gcaggagcat gacaatccgt
     1741 tcacgctcta tccttatgac accaactacc tcatttacac ccaaaccagc gatctgaata
     1801 aagaagcgat tgccagttac gactgggcgg aaaatgcgcg taaggatgaa gtaaagtttc
     1861 agttgagcct ggcatttccg ctgtggcgtg ggattttagg cccgaactcg gtgttgggtg
     1921 cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag cgaagagtct tcaccgtttc
     1981 gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc caccgattac cgttttgcag
     2041 gttggacgct gcgcgatgtg gagatggggt ataaccacga ctctaacggg cgttccgacc
     2101 cgacctcccg cagctggaac cgcctttata ctcgcctgat ggcagaaaac ggtaactggc
     2161 tggtagaagt gaagccgtgg tatgtggtgg gtaatactga cgataacccg gatatcacca
     2221 aatatatggg ttactaccag cttaaaatcg gctatcacct cggtgatgcg gtgctcagtg
     2281 cgaaaggaca gtacaactgg aacaccggct acggcggcgc ggagttaggc ttaagttacc
     2341 cgatcaccaa acatgtgcgc ctttatactc aggtttacag cggctatggc gaatcgctca
     2401 tcgactataa cttcaaccag acccgtgtcg gtgtgggggt tatgctaaac gatttgtttt
     2461 gatgaacggt tgagtggttg gcaaatctgg aatccagcat ccaggattac cctctcagag
     2521 actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt ttatttcagg
     2581 caatcggggt gaatgtggcg caggcggaag tgttgaatct ggagtccgga gctaaacagg
     2641 ttttacaaga aacctttggc taccaacagt ttcgccccgg ccaggaagaa attatcgaca
     2701 ctgtgctttc cggtcgcgat tgcctggtcg tcatgcccac cggtggcgga aaatcccttt
     2761 gctatcaaat tcctgcctta ttgctaaacg gccttaccgt ggttgtttca ccgctgattt
     2821 cgttgatgaa agatcaggtg gatcaactgc aagccaacgg cgtggcggcg gcgtgcctta
     2881 actcgacgca aacccgcgaa cagcaacttg aagtgatgac aggctgccgc accgggcaaa
     2941 ttcgtctgct ttatatcgcc ccggaacgcc tgatgctgga taactttctt gagcatctgg
     3001 cgcactggaa tccggtgtta ttagccgttg atgaagcgca ctgtatctcc caatggggcc
     3061 acgatttccg cccggaatat gccgcgctcg gtcagttgcg ccagcggttc ccgacgctgc
     3121 cgtttatggc gctgaccgcc acagccgacg acaccacgcg ccaggatatc gtgcgcctgc
     3181 tggggctgaa cgatccgctg attcaaatca acagttttga ccgtccgaat attcgctaca
     3241 tgctgatgga gaagttcaaa ccgctcgatc agttgatgcg ctacgtgcag gaacagcgcg
     3301 gtaagtccgg cattatctac tgcaacagcc gggcgaaagt agaagacacc gctgcgcgcc
     3361 tgcaaagcaa gggtattagc gcggcggcct atcatgccgg gctggaaaat aatgtccgcg
     3421 ccgacgtgca ggagaaattc cagcgcgatg acctgcaaat tgtggtggcg acggtggcgt
     3481 tcggcatggg catcaataaa ccaaacgttc gcttcgtggt ccactttgat attccgcgca
     3541 atatcgaatc ctattatcag gaaaccggtc gcgccgggcg tgatggcctg cccgcggaag
     3601 cgatgctgtt ttacgatccg gccgatatgg cgtggctgcg ccgttgtctg gaagagaagc
     3661 cgcaggggca gttgcaggat atcgaacgtc ataaactcaa tgcgatgggc gcttttgccg
     3721 aagcgcaaac ttgccgtcgt ctggtactgc tgaactattt tggcgaaggg cgtcaggagc
     3781 cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca gtacgacggt tcaaccgatg
     3841 ctcagattgc cctttccacc attggtcgtg tgaatcagcg ttttggtatg ggctatgtgg
     3901 tggaagtgat tcgcggcgct aataaccagc gtatccgcga ctatggtcat gacaaactaa
     3961 aagtctacgg tatgggccgt gataaaagtc atgaacattg ggtgagcgtg atccgccagt
     4021 tgattcacct cggcctggtg acgcaaaata ttgcccagca ttctgccctc caactgacag
     4081 aggccgcgcg tccggtgctg cgcggcgaat cctctttgca acttgccgtg ccgcgtatcg
     4141 tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg caactatgat cgcaaactgt
     4201 tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga aagtaatgtc ccgccgtacg
     4261 tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca gatgccgatc accgccagcg
     4321 aaatgctcag cgttaacggt gttgggatgc gcaagctgga acgctttggc aaaccgttta
     4381 tggcgctgat tcgtgcgcat gttgatggcg atgacgaaga gtagtcagca gcataaaaaa
     4441 gtgccaggat gaagactccg taaacgtttc ccccgcgagt caaatgtatg ttgatgttat
     4501 ttctcaccgt cgccatggtg cacattgtgg cgcttatgag ccccggtccc gatttctttt
     4561 ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc gatgatgggc gtgctgggca
     4621 ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct tggcctgcat ttgattatcg
     4681 aaaaaatggc ctggctgcat acgctgatta tggttggcgg tggcctgtat ctctgctgga
     4741 tgggttacca gatgctgcgc ggcgcgctga aaaaagaggc agtttctgca cctgcgccac
     4801 aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg tttactgacc aatctcgcta
     4861 atccgaaagc gattatctac tttggctcgg tgttctcatt gtttgtcggt gataacgttg
     4921 gcactaccgc gcgctggggc atttttgcgc tgatcattgt cgaaacgctg gcgtggttta
     4981 ccgtcgttgc cagcctgttt gccctgccgc aaatgcgccg tggttatcaa cgtctggcga
     5041 agtggattga tggttttgcc ggggcgttat ttgccggatt tggcattcat ttgattattt
     5101 cgcggtgatg ccagacgcgt cttcagagta agtcggataa ggcgtttacg ccgcatccga
     5161 cattattttt cacgcatgcc tcgccgatgc taacagcgct cccaccagca taaacaacga
     5221 gccgaaaatc ttattcagcg ccttcatctg ctttggtcct ttaatccata gagcaatccg
     5281 ttgagcaagg gtggcgtaac cgatcatcac aataatatcg accacaatag tggtgacgcc
     5341 gagcacgata tactgcatca gttgcggctg ttgcggcatg atgaattgcg gaaatagcgc
     5401 cgccagaaac acaatacttt tgggattggt gagattcaca aaaactgcgc gctggaacaa
     5461 atgtcgacgc gattgagtag aggccagcga tttaaggtca attgcaccag cggcgcgcca
     5521 ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc cacttcaaca cttcaaacgc
     5581 aatcactgag cgggaaaata gcgtccccaa ccccacgcca accagcacaa tatgaatcgc
     5641 cagtccggtt tgaagcccag caatagacgc caccgcgccg cgataaccgt ggttgagcga
     5701 ggtggtcata gtgttgattg caccagagcc tggcgacagg cttaaaatga tcgatgtcag
     5761 caggtaggca aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct atttttgtgc
     5821 cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta aaaaaaacga ttttacgtgg
     5881 tttaagaggc agattacccg atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg
     5941 cgtttgctgc ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt gatgaagcag
     6001 agtttactgg tgtggatgac attccggtgc gctttgtccg ttttcgcgca cagcaccatg
     6061 accgggtggt agtcatctgc ccggggcgta ttgagagcta cgtaaaatat gcggaactgg
     6121 cctatgacct gttccatttg gggtttgatg tcttaatcat cgaccatcgc gggcagggac
     6181 gttccggtcg cctgttagcc gatccgcatc tcgggcatgt taatcgcttt aatgattatg
     6241 ttgatgatct ggcggcattc tggcagcagg aggttcagcc cggtccgtgg cgtaaacgct
     6301 atatactggc acattcgatg ggcggtgcga tctccacatt atttctgcaa cgccatccag
     6361 gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt cgtatgccgt
     6421 catttatggc acggcagatc ctcaactggg ccgaagcgca tccacgtttc cgtgatggct
     6481 atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta ctgacccaca
     6541 gcagacagcg atatcgacgt aacttacgct tctatgctga tgacccaacg attcgcgtcg
     6601 gtgggccgac ctaccattgg gtacgcgaaa gtattctggc tggcgaacag gtgttagccg
     6661 gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc tgaagaggaa cgcgtggtgg
     6721 ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat cctgtcgaag
     6781 gaggacgacc gttggtaatt aaaggtgctt accatgagat cctttttgaa aaggacgcaa
     6841 tgcgctcagt cgcgctccac gccatcgttg attttttcaa caggcataac tcacccagcg
     6901 gaaaccgctc tacagaggtt taaatttctt atgtaccagg ttgttgcgtc tgatttagat
     6961 ggcacgttac tttctcccga ccatacgtta tccccttacg ccaaagaaac tctgaagctg
     7021 ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt tgatgtgggg
     7081 caaattcgcg ataatctgga gattaagtct tacatgatta cctccaatgg tgcgcgcgtt
     7141 cacgatctgg atggtaatct gatttttgct cataacctgg atcgcgacat tgccagcgat
     7201 ctgtttggcg tagtcaacga caatccggac atcattacta acgtttatcg cgacgacgaa
     7261 tggtttatga atcgccatcg cccggaagag atgcgctttt ttaaagaagc ggtgttcaaa
     7321 tatgcgctgt atgagcctgg attactggag ccggaaggcg tcagcaaagt gtttttcacc
     7381 tgcgattccc atgaacaact gctgccgctg gagcaggcga ttaacgctcg ctggggcgat
     7441 cgcgtcaacg tcagtttctc taccttaacc tgtctggaag tgatggcggg cggcgtgtcg
     7501 aaaggccatg cgctggaagc agtggcgaag aaactgggct acagcctgaa ggattgtatt
     7561 gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga tggcggggaa aggctgcatt
     7621 atgggcagtg cgcaccagcg tctgaaagac cttcatcccg agctggaagt gattggtact
     7681 aacgccgaag acgcggtgcc gcattatctg cgtaaactct atttatcgta atcgttcttt
     7741 atttggtcag ttgtcaacct gatacttcgc tacaatggat actcgttaat caaagagttt
     7801 tccattgtgg cgctacttat catcaccacg attctgtggg ccttctcctt tagcttttat
     7861 ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg cgttggcctg
     7921 gcggcactcg tttttctgcc gtttctgcgt acccgtggca atagcctgaa aacagtcggc
     7981 ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag tttccgcgct
     8041 tatctctacc tgacggtttc cgagctgctg ctgttcaccg tgctgacgcc gctctacatc
     8101 acgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggctatgc ttttagcgcc
     8161 ttgctggcgg tgattggtgc cgggattatt cgctatgatc aggtcaccga ccatttctgg
     8221 actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac
     8281 aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt
     8341 ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg
     8401 caaaccacgc tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc
     8461 tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg cattatgaat
     8521 aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca acagccgcac
     8581 tggccaacgt ttattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag
     8641 tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc
     8701 gaataaacgc ttccgtaact ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc
     8761 ggctccacaa gccttcgccc agggttttgg tcaccaccag accctggcgc tcaaaactct
     8821 ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca
     8881 ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga agaaaatgcc
     8941 gccagacatc cagtcgacta cgctgcaccg gataaatcaa cagcgtctcg ctggcgagat
     9001 cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc aggtgctaac accagacgca
     9061 cttcatagtc gaacatcggc gaataatgca ggccactgcg cggcaggata tcggaggtca
     9121 tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg gtcaaacgtc acgcccgatt
     9181 taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaacgcg ggcgtcagcc
     9241 actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc ggttcattgc
     9301 aggcttgcag tgcctggcta atttgcggca gcacctggtt tgccagttgc aacaggattt
     9361 ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg aagccaaggc
     9421 gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc aacgtcgccg
     9481 cagcggctgc gagagagccg cagttccgca acgcttgtag cgttttcagg tgttttactt
     9541 cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac
     9601 cgccaattat ggatgtgtaa acatctggac ggctaaatac taaaaattcc ataaggggca
     9661 tataatgaca attcttaatc acaccctcgg tttccctcgc gttggcctgc gtcgcgagct
     9721 gaaaaaagcg caagaaagtt attgggcggg gaactccacg cgtgaagaac tgctgacagt
     9781 agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag caagcgggta tcgacctgct
     9841 gccggtgggc gattttgcct ggtacgatca tgttctgacc accagtctgc tgctgggtaa
     9901 tgttccgcca cgtcatcaga acaaagacgg ttcggtagat atcgacaccc tgttccgtat
     9961 tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt
    10021 taacaccaac tatcactaca tggtgccgga attcgttaaa ggccaacagt tcaaactgac
    10081 ctggacgcag ctgctggagg aagtggacga ggcgctggcg ctgggccaca acgttaagcc
    10141 tgtgctgctg gggccggtta cttacctgtg gctggggaaa gtgaaaggtg aacagtttga
    10201 ccgccttagc ctgctgaacg acattctgcc ggtttatcag caagtgctgg cagaactggc
    10261 gaaacgcggc atcgagtggg tacagattga tgaacccgcg ctggtactgg aactaccaca
    10321 ggcgtggctg gacgcataca aacccgctta cgacgcgctc cagggacagg tgaaactgct
    10381 gctgaccacc tattttgaag gcgtaacgcc aaatctcgac acgattactg cgctgcctgt
    10441 tcagggtctg catgttgacc tcgtacatgg taaagatgac gttgctgaac tgcacaagcg
    10501 cctgccttct gactggttgc tgtctgcggg tctgatcaat ggtcgtaacg tctggcgcgc
    10561 cgatcttacc gagaaatatg cgcaaattaa ggacattgtc ggcaaacgtg atttgtgggt
    10621 ggcatcttcc tgctcgttgc tgcacagccc catcgacctg agcgtggaaa cgcgtcttga
    10681 tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa tgccatgaac tggcactgct
    10741 gcgcgatgcg ctgaacagtg gtgacacggc agctctggca gagtggagcg ccccgattca
    10801 ggcacgtcgt cactctaccc gcgtacataa tccggcggta gaaaagcgtc tggcggcgat
    10861 caccgcccag gacagccagc gtgcgaatgt ctatgaagtg cgtgccgaag cccagcgtgc
    10921 gcgttttaaa ctgccagcgt ggccgaccac cacgattggt tctttcccgc aaaccacgga
    10981 aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc gacgccaata actaccgcac
    11041 gggcattgcg gaacatatca ggcaggccat tgttgaacag gaacgtttgg gactggatgt
    11101 gctggtacat ggcgaggccg agcgtaatga catggtggaa tactttggcg agcacctcga
    11161 cgggtttgtc tttacgcaaa acggttgggt acagagctac ggttcccgct gcgtgaagcc
    11221 accgattgtt attggtgacg ttagtcgccc ggcaccgatt accgtggagt gggcgaagta
    11281 tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg acggggccgg tgaccatact
    11341 ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc atcgccaaac agattgcgct
    11401 ggcgctgcgt gatgaggtgg ccgatctgga agcggcaggg attggtatca tccagattga
    11461 cgaaccggcg ctgcgcgaag gtttaccact gcgtcgtagc gactgggacg cctatctcca
    11521 gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca
    11581 cactcacatg tgttattgcg agttcaacga catcatggat tcgattgcgg cgctggacgc
    11641 agacgtcatc accatcgaaa cctcgcgttc cgacatggag ttgctggagt cgtttgaaga
    11701 gtttgattat ccaaatgaaa tcggtccagg cgtctatgac attcactcgc caaacgtacc
    11761 gagcgtggaa tggattgaag ccctgctgaa gaaagcggca aaacgcattc cggcagagcg
    11821 cctgtgggtc aacccggact gtggcctgaa aacgcgcggc tggccagaaa cccgcgcagc
    11881 actggcgaat atggtgcagg cggcgcagaa cttgcgtcgg gggtaaatcc aaaccgggtg
    11941 gtaataccac ccggtctttt ctcattacag cgacttcttc ccaccatact gcttaaacca
    12001 ttccagcata cgctgccagc catctttcgc agattcggca tgatagctcg ggcgataatc
    12061 agcgttgaat gcatgcccgg catccgggta cacgataatc tctgctttcg cattagcagc
    12121 ccgcagcgcc tggcgcatgg tttcaacgct ctcctgcgga atgctgttat cctgaccgcc
    12181 atataagccg agaaccggcg cgttaagatc ggttgcgata tcaacaggtt gtttcggtga
    12241 attcagcgac ttgtcacccg tcagtttgcc gtaccacgcc actgcggctt ttagctgtgg
    12301 attatgcgcg gcatacagcc aggtgatacg tccaccccag cagaatccgg tgatcattaa
    12361 acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg acatgatcga gatcggccag
    12421 cacctgcgag tcaggcactt ttgctaccag accgctaagc aacgtgggga tatcggcaaa
    12481 atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg atagccagat acccctccag
    12541 cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg ccaaaaattt cctgcactac
    12601 aatgaccact ggcagtgggc catcgctttg ctttggtctg gcatggtaag caggcatgtt
    12661 atccccttgt gaagggatag aggtgaagcc cgccacaatc gcgtcgtccg gggtctgaac
    12721 gatggtagaa gcgagaggcg atgcagcagg tgcaaatcca gattgttgtg ttgttgccat
    12781 ggtattctcc gtacccttat aaaaatgttg cgcaatgtta actatagtca gcatgcaaca
    12841 aatcacattg cctgaatcgg ctcatctttt atgcagtcct gcagaatgaa gagtgattta
    12901 tgtgatttac atcacttttg atgggtaaat ttatgcaacg catttgcgtc atggtgatga
    12961 gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt tttgcttctt ctgactaaac
    13021 cgattcacag aggagttgta tatgtccaag tctgatgttt ttcatctcgg cctcactaaa
    13081 aacgatttac aaggggctac gcttgccatc gtccctggcg acccggatcg tgtggaaaag
    13141 atcgccgcgc tgatggataa gccggttaag ctggcatctc accgcgaatt cactacctgg
    13201 cgtgcagagc tggatggtaa acctgttatc gtctgctcta ccggtatcgg cggcccgtct
    13261 acctctattg ctgttgaaga gctggcacag ctgggcattc gcaccttcct gcgtatcggt
    13321 acaacgggcg ctattcagcc gcatattaat gtgggtgatg tcctggttac cacggcgtct
    13381 gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg aattcccggc tgtcgctgat
    13441 ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta ttggcgcgac aactcacgtt
    13501 ggcgtgacag cttcttctga taccttctac ccaggccagg aacgttacga tacttactct
    13561 ggtcgcgtag ttcgtcactt taaaggttct atggaagagt ggcaggcgat gggcgtaatg
    13621 aactatgaaa tggaatctgc aaccctgctg accatgtgtg caagtcaggg cctgcgtgcc
    13681 ggtatggtag cgggtgttat cgttaaccgc acccagcaag agatcccgaa tgctgagacg
    13741 atgaaacaaa ctgaaagcca agcggtgaaa atcgtggtgg aagcggcgcg tcgtctgctg
    13801 taattctctt ctcctgtctg aaggccgacg cgttcggcct tttgtatttt tgcgtagcgc
    13861 ctcgcaggaa atgcctttcc aactggacgt ttgtacagca caattctatt ttgtgcgggt
    13921 aagttgttgc gtcaggaggc gttgtggatt tctcaatcat ggtttacgca gttattgcgt
    13981 tggtgggtgt ggcaattggc tggctgtttg ccagttatca acatgcgcag caaaaagccg
    14041 agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa caacaaatta
    14101 cccaaagcga gcactggcgt gcagagtgcg agttactcaa taacgaagtg cgcagcctgc
    14161 aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg atggaagccg
    14221 cacagcaaca tgctgacgat aaaattcgcc agatgattaa cagcgagcag cgcctcagtg
    14281 agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg gttgatgagc
    14341 aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg gacggtttcc
    14401 gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga acgccatacc ctgacccacg
    14461 aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca ggaagcgatc aacctgacgc
    14521 gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtagta ttgacgcggg
    14581 tgctggaggc ttccggtctg cgtgaagggt atgaatatga aacccaggtc agcatcgaaa
    14641 atgacgcccg ctcgcggatg caaccggatg tcatcgtgcg gctgccgcag ggaaaagatg
    14701 tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctatttcaac gccgaagatg
    14761 actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac catatccgtt
    14821 tgctgggacg caaagattat caacagctgc cggggctgcg aactctggat tacgtgctga
    14881 tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg gagctgatca
    14941 ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac tacgctgctg gtggcgttgc
    15001 gtactattgc caacctgtgg cgttatgagc atcaaagccg caacgcccag caaatcgccg
    15061 atcgtgccag caagctgtac gacaagatgc gtttgttcat cgatgacatg tccgcgattg
    15121 gtcaaagtct cgacaaagcg caggataact accgccaggc aatgaaaaaa ctctcttcag
    15181 gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggagta gaaattaaac
    15241 gcgagattaa tccggatttg gctgaacagg cggtgagcca ggatgaagag tatcgacttc
    15301 ggtcggttcc ggagcagccg aatgatgaag cttatcaacg cgatgatgaa tataatcagc
    15361 agtcgcgcta gcccattagg ggtagttaag ccgggtagaa atctagggca tcgacgccca
    15421 atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt ggataagtca
    15481 caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc ggatatggtc
    15541 gcccacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt gatgtcattt
    15601 ggtattcatc gtttgtggaa gcgattcact attgattgca gcggcgtacg ccgtgggcag
    15661 accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc ccgcctggtc
    15721 ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa aatgggacgc
    15781 gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc gaacgctgag
    15841 gcgctgccgt tcccggataa cacctttgat tgcatcacca tttcgtttgg tctgcgtaac
    15901 gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc cggcggccgc
    15961 ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc ctatgatgca
    16021 tactccttcc atgtgctgcc gcgtattggc tcactggtcg cgaacgacgc cgacagctac
    16081 cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa agccatgatg
    16141 caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt tgtggcgctg
    16201 catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt tagtgacggc
    16261 aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga aaacagcccg
    16321 ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa ggcttttcga cgtcattgat
    16381 tctggtgttc agcgaacgcc aggttgatgt actgggcgaa tgggcaggcg atgctgactg
    16441 caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc gatcgccagc agcttaccgc
    16501 actgattcgc agtggtgagc tggaagtgca gggcgatatt caggtggtgc aaaacttcgt
    16561 tgcgctggca gatctggcag agttcgaccc tgcggaactg ctggcccctt ataccggtga
    16621 tatcgccgct gaaggaatca gcaaagccat gcgcggaggc gcaaagttcc tgcatcacgg
    16681 cattaagcgc cagcaacgtt atgtggcgga agccattact gaagagtggc gtatggcacc
    16741 cggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc gtgctgttga
    16801 tgccctgacc aaacggctgg aaaaactgga ggctaaatga cgccaggtga agtacggcgc
    16861 ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat ccccaaaatg
    16921 cgtatcaccc tgccgctacg gctatggcga tactcattat tctggatgcc aaatcggcat
    16981 aaagacaaac ttttaggtga gcgactacga ctggccctgc aagaactggg gccggtttgg
    17041 atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt ttccaccgca tattgccgat
    17101 cagctggcgt tattgcagga caaagttgct ccgtttgatg gcaagctggc gaagcagcag
    17161 attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt tgaaatcaag
    17221 ccgctggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc gaatggtaaa
    17281 gaggtggtga ttaaagtcat ccgcccggat attttgccgg ttattaaagc ggatctgaaa
    17341 cttatctacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg ccgtctgcgc
    17401 ccaaccgaag tggtgcgcga gtacgaaaag acattgattg atgaactgaa tttgctgcgg
    17461 gaatctgcca acgccattca gcttcggcgc aattttgaag acagcccgat gctctacatc
    17521 ccggaagttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg catttacggc
    17581 attccggtgt ctgatgttgc ggcgctggag aaaaacggca ccaacatgaa attgctggcg
    17641 gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt ccatgccgat
    17701 atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa atatatcggc
    17761 attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct ggcagaaaac
    17821 tttatcgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt cgattctggc
    17881 tgggtgccac cagataccaa cgttgaagag ttcgaatttg ccattcgtac ggtctgtgaa
    17941 cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt aaatctgttt
    18001 aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact ccagaaaacc
    18061 ctgctctacg tcgaaggggt aggacgccag ctttatccgc aactcgattt atggaaaacg
    18121 gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc gctggtgaga
    18181 gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc tgaactggtt
    18241 tacgacagtt tgcgccaggg caagtattta cagcacagtg ttgataagat tgcccgcgag
    18301 cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat tggcgctacg
    18361 ttagtattaa gtggcacatt cttgttggtc agccgacctg aatgggggct gatgcccggc
    18421 tggttaatgg caggtggtct gatcgcctgg tttgtcggtt ggcgcaaaac acgctgattt
    18481 tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc cttgtttaat catcatctac
    18541 cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat tgccgtcatc
    18601 gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct tggtgcgtcg
    18661 atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa aaccagtcag
    18721 gatgctgatt ttactgcgaa aactatcgcc gataagcagg cgaatacgaa tcaggaacag
    18781 gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt gtttgatatc
    18841 ggttttagcg aactgctatt ggtgttcatc atcggcctcg tcgttctggg gccgcaacga
    18901 ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg cgttgcgttc actggcgaca
    18961 acggtgcaga acgaactgac ccaggagtta aaactccagg agtttcagga cagtctgaaa
    19021 aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc gatggatgaa
    19081 ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc tgaaaaggcg
    19141 agcgatgaag cgcacaccat ccataacccg gtggtgaaag ataatgaagc tgcgcatgag
    19201 ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc cggaacagaa gccagaaacc
    19261 acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc tgcaccttcc
    19321 ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg cttatcacgc
    19381 atctaattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc gtgatattcc
    19441 tgtgtctggt ctatttcgcc aatgacatct atcacctggt atccgcgcca ttgatcaagc
    19501 agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc tttacgccga
    19561 tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc tatcaggtgt
    19621 gggcatttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg ccgctgctgg
    19681 tttccagctc gctgctgttt tatatcggca tggcgttcgc ctactttgtg gtctttccgc
    19741 tggcatttgg cttccttgcc aataccgcgc cggaaggggt acaggtatcc accgacatcg
    19801 ccagctattt aagcttcgtt atggcgttgt ttatggcgtt tggtgtctcc tttgaagtgc
    19861 ctgtggcaat tgtgctgctg tgctggatgg ggattacctc gccagaagac ttacgcaaaa
    19921 aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gctgctgaca ccgccggatg
    19981 tcttctcgca aacgctgttg gcgatcccga tgtactgtct gtttgaaatc ggtgtcttct
    20041 tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac gctgaagcag
    20101 aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc atatggagta
    20161 caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag accgtgatga
    20221 tgttgtagcg cgcgcttttg acgcgggagt taatgggcta ctcatcaccg gcactaacct
    20281 gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat tcgtcctgtt ggtcaacggc
    20341 gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag cgattattga
    20401 gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact ttaaccgcaa
    20461 cttttcgacg ccggaagagc aggaacgcgc ttttgttgct cagctacgca ttgccgcaga
    20521 attaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta tgacattgct
    20581 ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg gcacacgcga
    20641 agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc attaccggtt gggtttgcga
    20701 tgaacgacgc gggctggagc tgcgggaatt gttgccgttg attccggcgg agaaattgct
    20761 gatcgaaact gatgcgccgt atctgctccc gcgcgatctc acgccaaagc catcatcccg
    20821 gcgcaacgag ccagcccatc tgccccatat tttgcaacgt attgcgcact ggcgtggaga
    20881 agatgccgca tggctggctg ccaccacgga tgccaatgtc aaaacactgt ttgggattgc
    20941 gttttagagt ttgcggaact cggtgttctt cacactgtgc ttaatctctt tattaataag
    21001 attaagcaat agcatggagc gagcctcacc atcgggttcg gtgaaaatgg cctgaaagcc
    21061 ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggttg ccggatcgac
    21121 aatgtctttc ggtttatata ccgatagctg atgaatcacc gccgatggga ctatcgctgg
    21181 cgacgcgcca aagcgcacga agtggctgac accgcgggtc gcgttgatag tcgtggtatg
    21241 aatcacttct ggatcaaatt cgacaaacag gtagttggga aacaatggct cactgactgc
    21301 agtacgtttt ccacgcacga ttttttccag ggtgatcatc ggtaccaggc aattcacagc
    21361 ctgtctttcg aggtgttcct gggcacgttg aagttgcccg cgcttgcagt acagtaaata
    21421 ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc aaaagcttta
    21481 cgctaagtta attatattcc ccggtttgcg ttataccgtc agagttcacg ctaatttaac
    21541 aaatttacag catcgcaaag atgaacgccg tataatgggc gtagattaag aggctacaat
    21601 ggacgccatg aaatataacg atttacgcga cttcttgacg ctgcttgaac agcagggtga
    21661 gctaaaacgt atcacgctcc cggtggatcc gcatctggaa atcactgaaa ttgctgaccg
    21721 cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac cctaaaggct actcaatgcc
    21781 ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg atgggcatgg ggcaggaaga
    21841 tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt ctgaaagagc cggagccgcc
    21901 aaaaggtttc cgcgacctgt ttgataaact gccgcagttt aagcaagtat tgaacatgcc
    21961 gacaaagcgg ctgcgtggtg cgccctgcca acaaaaaatc gtctctggcg atgacgtcga
    22021 tctcaatcgc attcccatta tgacctgctg gccggaagat gccgcgccgc tgattacctg
    22081 ggggctgaca gtgacgcgcg gcccgcataa agagcggcag aatctgggca tttatcgcca
    22141 gcagctgatt ggtaaaaaca aactgattat gcgctggctg tcgcatcgcg gcggcgcgct
    22201 ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt ttcccggttt ctgtggcgct
    22261 gggtgccgat cccgccacga ttctcggtgc agtcaccccc gttccggata cactttcaga
    22321 gtatgcgttt gccggattgc tgcgcggtac caagaccgaa gtggtgaagt gtatctccaa
    22381 tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa gggtatatcg aacaaggtga
    22441 aacggcgccg gaagggccgt atggcgacca caccggttac tataacgaag tcgatagttt
    22501 cccggtattt accgtgacgc atattaccca gcgtgaagat gcgatttacc attccaccta
    22561 taccgggcgt ccgccagatg agcccgcggt actgggcgtg gcgttgaacg aagtgtttgt
    22621 accgattctg caaaaacagt tcccggaaat tgtcgatttt tacctgccgc cggaaggctg
    22681 ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac gccggacacg cgaagcgcgt
    22741 catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg tgatcgtttg
    22801 cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt tgggcgatta ccacccgtat
    22861 ggacccggcg cgggatactg ttctggtaga aaatacgcct attgattatc tggattttgc
    22921 ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat gccacgaata aatggccggg
    22981 ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg tcgcgcatat
    23041 tgacgccatc tgggatgaac tggctatttt taacaacggt aaaagcgcct gatgcgcgtt
    23101 tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa gctgtaaagt
    23161 gacctcggta gaagctatca cggataccgt atatcgtgtc cgcatcgtgc cagacgcggc
    23221 cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg acaaacgtcc
    23281 gttctcaatg gcctcgacgc cggatgaaaa agggtttatc gagctgcata ttggcgcttc
    23341 tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc ctcaaagatc atcaaatcgt
    23401 ggtcgatatt ccccacggag aagcgtggct gcgcgatgat gaagagcgtc cgatgatttt
    23461 gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt ttgctgacag cgttggcgcg
    23521 taacccaaac cgtgatatca ccatttactg gggcgggcgt gaagagcagc atctgtatga
    23581 tctctgcgag cttgaggcgc tttcgttaaa gcaccctggt ctgcaagtgg tgccggtggt
    23641 tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc gtgttaacgg cggtattgca
    23701 ggatcatggt acgctggcag agcatgatat ctatattgcc ggacgttttg agatggcgaa
    23761 aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgcc tgtttggcga
    23821 tgcgtttgca tttatctgag atataaaaaa acccgcccct gacaggcggg aagaacggca
    23881 actaaaactt tattcagtgg catttagatc tatgtcgcat ccggcaattg gtgcacgacg
    23941 cctgatgcga cgctggcgcg tcttatcagg cctacattgg tgccggatcg gtagaccgga
    24001 taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc tcaaacaccg tcgcaatacc
    24061 ctgacccaga ccgatacaca tcgtcgccag accaaactga acgtctttgc gttccatcag
    24121 attcagcagc gtggtgctga tacgcgcacc ggaacagccc agcggatgac ccagcgcgat
    24181 cgcaccacca ttgaggttga tcttctcgtc aatctgctcc attagcccga gatctttaat
    24241 gcacggcagg atctgcgcgg caaaggcttc gttcatctca aacacgccga tatcgctggc
    24301 agaaagcccc gcttttttca gcgccagctt cgaggccgga accgggccgt aacccataat
    24361 cgatgggtca caaccaacga ccgccatcga acgcacacga gcgcgcggct taagacctaa
    24421 ttcatgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg aaagtgcaga
    24481 agatgtgccc gccgttaccg taccgtttac tggatcaaac gccggacgca gcgtggcgag
    24541 ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa ttaaactgct tcaggacgcc
    24601 gtcggcatcg tgaccaccag ttgggatgat ttcattttta aatgcggccg actgcgtggc
    24661 ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc tgcatttcac ggctgatacc
    24721 gtgcatacgc gccagcattt ctgccgttaa gcccatcatg cccgccgctt tggcgacatt
    24781 gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc ggcacatggc ccatatgctc
    24841 cacgccgcca accagacatg cctgcgcatc gccagtcatg atcattcgtg ctgcgtcatg
    24901 cagtgcctgc atggatgaac cacacaagcg attgacggta accgccggga cagagtgcgg
    24961 cacttctgcc agcagcgccg cgttacgggc gatattaaaa ccctgctcca gcgtctgctg
    25021 cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc agcgccgggt tacgcgccag
    25081 caggctacgc attaaatgag cggagagatc ttctgcacgc acgttacgaa aagcaccgcc
    25141 cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg acaacctgtt ccattgtgac
    25201 tccttaagcc gttttcaggt cgccaaccgg acgggctggc tcaaccgggg gatagtacgg
    25261 ttcgttatga cgcgctttat tacgcagacc ttccggcact tcatacagcg ggccgaggtg
    25321 ctgatattgc tgtgccatat cgaggtattt tgcgctaccg agggtgtcca gccagcggaa
    25381 cgcgccgccg tggaacggag ggaagcccag gccgtagacc agcgccatat ccgcttccgc
    25441 cggagtggcg ataatgcctt cctccagaca gcgcaccact tcgttgacca tcgggatcat
    25501 catgcgggcg ataatctctt cttcgctgaa atcgcgcttc ggctggctca cttctgccag
    25561 caggtcttca acggcggcgt cttcttcttt cttcggctta cctttgctgt cttctttata
    25621 acgccagaaa ccgaggccgt tcttctgacc aaagcggttg gcatcaaaca gcgcgtcgat
    25681 ggcatcgcgg taatctttct gcatccgctg cgggaagcct gctgccatga cagcctgagc
    25741 gtgatgcgcg gtatcaatgc ccacgacatc cagcagatat gccggaccca tcggccagcc
    25801 aaactgtttt tccatcactt tgtcgatctt gcggaaatcc gcgccgtcgc gcagcagctg
    25861 gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca aagaagccgg ggcagtcgtt
    25921 aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg acaactttcg cgatggtttc
    25981 gtcggagctt ttctcgccgc gaataatttc taccaacggc atccggtgta ccgggttaaa
    26041 aaagtgcatt ccgcagaagt tttccgggcg ttccagcgcg ttggccagtt cgctgatagg
    26101 aatggttgaa gtgttagacg ccagcacggt atccgggcgt actttttgtt cggtttctgc
    26161 cagtacggct tttttcactt tcgggttttc gacaaccgct tctaccacaa catccacgcg
    26221 gtcaaacccg gcgtagtcga gcgttgggtg gattgtggag atcacgccag ccagtttcag
    26281 accatcgatc ttgccgcgct caagctgctt gttcagcagt ttcgcggctt cggtcatgcc
    26341 gagggttaac gacttgtcgt tgatatcttt catgacaacc ggcacgcctt tccacgcaga
    26401 ctggtaagcg atgccgccgc ccataatgcc tgcacccagc accgcggcct gtttcggggt
    26461 ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca tattgatcgt taaggaaaat
    26521 gccgaccagt gcgcgggctt cgttggtatg cgccagcggg acaaaacttt tgttttccag
    26581 gtttaaggct tcttcacgac caaaacgggc cgcagcttca atggttttta ctgcggtgat
    26641 gggggccgga taatgtttcc ccgctgtttg tgcgaccatc cctttagcga tggtgaagct
    26701 catggtggct tcaatcttgc tcagttttag tggttccagc ttcggctgac gttttgcttt
    26761 ccagtcgagg tcgccgttaa tggcctggcg taaaaccgcc tttgcgcctt caaccagttt
    26821 ttctgctttg actacgccat ccaccagacc gattttcagc gcctgatccg cgccgacatc
    26881 tttaccggcg gcaatgattt ccagcgcact gtcagcgccc agcatacgtg gcatacgtac
    26941 agaaccgcca aagcccggca tgatgcccag tttggtttcc ggcagaccga tgcgcagatc
    27001 cggcgtcgcc agacgataat cggtcgccag cacgcattcg cagccaccgc ccagcgcata
    27061 gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc aggcgattaa acacgctatt
    27121 ggcaaagtgc agccactgac ttaactgttc ttcaggaacg aggaacaggg acaaaaattc
    27181 ggtgatatca gcaccgacga taaaggctgc tttgttcgaa cgcagcagca gcccttttag
    27241 atctgattgc tgttccagca cgccgatggc ctcgccgagg ctggcgacgg tcgcagtgtc
    27301 gagtttatta actgaacctg gggcatcaaa taccagttcg gcaatgccat cttccagcca
    27361 gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc tcctgaatcc gcaaggtgat
    27421 ctggtcgtac cagatgcatc gaagtgtgta ttttgtgtta aaaatatgca aataaaagat
    27481 taaagaaatg ccgatctgat cacgctcggc agaaatcacg ctctggatga acgatgtgct
    27541 aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa aaatggaatc actggcctca
    27601 ctctataaaa atcatatagc taccttacag gaacggactc gcgatgcgct ggcgcgcttc
    27661 aagctggatg cgttacttat tcactccggc gaactgttca acgtttttct cgacgatcat
    27721 ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg tgccggtaac tcaggtgcca
    27781 aactgctggc tgctggtgga tggcgtgaac aagccgaaac tgtggttcta tctgccggtt
    27841 gattactggc acaacgtcga accgctgccg acctctttct ggactgaaga cgtggaagtg
    27901 atcgcgctgc cgaaagccga tggcattggt agcctgctgc ctgctgcgcg cgggaatatc
    27961 ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg gtattgaggc cagcaatatc
    28021 aacccgaaag gggtgatcga ctacctgcat tactaccgct ccttcaaaac cgagtacgaa
    28081 ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaacg gtcatcgcgc ggcagaagaa
    28141 gcgttccgtt ctggcatgag cgagttcgat atcaacattg cctatctgac cgcgaccggt
    28201 catcgtgata ccgacgtacc ttacagcaac attgtggcgc tcaacgaaca cgctgcggtg
    28261 ttgcattaca ccaaactgga ccaccaggca ccggaagaga tgcgcagctt cctgctggat
    28321 gccggggccg aatataacgg ctatgcggct gacctgaccc gtacctggtc ggcaaaaagt
    28381 gacaacgact acgcacagct ggtgaaagac gtaaatgatg aacaactggc gctgatcgct
    28441 accatgaaag caggcgtcag ctatgtggat taccacatcc agttccatca gcgcatcgcc
    28501 aaactgctgc gtaaacatca aatcatcacc gatatgagtg aagaagcgat ggtcgaaaac
    28561 gatctcaccg gaccgtttat gccgcacggt attggccatc cgctgggcct gcaggtgcat
    28621 gacgtagccg gttttatgca ggatgatagc ggtacgcacc tcgcggcacc ggcaaaatat
    28681 ccgtacctgc gctgcacccg tattctccag ccgggcatgg tgttaaccat cgaaccgggt
    28741 atctacttca ttgaatcgct gctggcaccg tggcgtgaag ggcagttcag caagcacttc
    28801 aactggcaga aaattgaagc actgaaaccg ttcggcggca ttcgtatcga agacaacgtg
    28861 gtgatccacg aaaacaacgt ggaaaacatg acccgggatc tgaaactggc gtgatggaaa
    28921 gctggttaat tcctgcggca ccggtcacgg tcgttgaaga gatcaaaaag agccgcttca
    28981 taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa agcgtttgtt gaatcggtgc
    29041 gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg ggtggcgggt gcgccggatg
    29101 attctcaaca gctgggtttc tctgacgacg gggagccggc gggaacggca ggtaaaccga
    29161 tgctcgccca gctaatgggc agcggcgtcg gggaaattac cgctgtggta gtgcgctact
    29221 acggcggcat attgcttggc accggtgggt tagtgaaagc gtatggcggc ggcgtgaatc
    29281 aggcgctgcg ccagctaacg acccaacgca agacgccatt aaccgaatat actttgcaat
    29341 gtgaatatag tcagttaacc ggcattgaag cgttgctggg gcagtgtgac ggcaaaatta
    29401 tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc gcttccggcg gcgaaagtgg
    29461 ctgaattttc cgcaaagctg gcggatttta gccgtggttc attgcaattg ttagcgattg
    29521 aagaataatc cccacttcgt tttgcagaac taaggaagcg gcagagatgc attttcgcgc
    29581 cattacccga atcgttggac tactggtcat cttattttca gggaccatga ttatccctgg
    29641 gctggtagca ctcatctacc gggatggagc gggccgcgct tttacccaga ccttttttgt
    29701 cgccctcgcc attggctcta tgctgtggtg gccgaaccgc aaagagaaag gcgaacttaa
    29761 atcccgtgag gggtttctga tagtggtgct gttctggacc gtgctgggta gcgtcggtgc
    29821 gctccctttt atcttctcgg aaagcccgaa cctcacgatt accgatgcgt tttttgaatc
    29881 tttctctggc ctgaccacca ccggggccac tacgctggtg gggctggatt cgctccctca
    29941 cgccatcctc ttttatcgcc agatgctgca atggtttggc gggatgggga tcatcgtgtt
    30001 agcggttgcg atactgccta tcctcggcgt gggtgggatg cagctctatc gcgcagaaat
    30061 gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt gcggaaacgg cgaaaaccct
    30121 gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg gcgttgtggt ttgccggaat
    30181 ggatgccttt gatgccatcg gccatagctt tgcgactatc gctattggcg gcttctcgac
    30241 acatgatgcc agtatcggtt atttcgatag cccgactatt aacactatca ttgctatctt
    30301 cctgctgatc tccggctgta actacggtct gcacttttca ctgttaagtg ggcgtagtct
    30361 gaaggtttat tggcgcgatc cggaatttcg catgtttatc ggcgtacagt ttacgctggt
    30421 ggttatttgt accctcgtac tgtggtttca taatgtctac agttcggcgc tgatgacaat
    30481 taaccaggcg tttttccagg tggtgtcgat ggcgacaacc gccgggttta cgactgacag
    30541 cattgcccgc tggccgctct ttttgccggt actgctttta tgttcggctt ttatcggcgg
    30601 ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc atcctgctgc tgtttaagca
    30661 ggggaaccgt gagctgaaac gactggtgca tccgaacgcc gtctatagca ttaagctggg
    30721 gaatcgcgca ctgccggaac gtatcctcga agccgtttgg ggatttttct ccgcctatgc
    30781 attggtgttt attgtcagta tgctggcgat tatcgccacg ggcgtggatg acttttctgc
    30841 ctttgcctca gttgttgcga cattgaataa cctggggcca gggcttggcg tggttgctga
    30901 taactttacc agtatgaacc cggtggctaa atggatcctg attgccaaca tgctgtttgg
    30961 tcgtctcgag gtctttacat tgctggtgct ctttaccccg actttctggc gtgaatgatg
    31021 gagtaatacg tgaaaacatt aattcttttc tcaacaaggg acggacaaac gcgcgagatt
    31081 gcctcctacc tggcttcgga actgaaagaa ctggggatcc agacggatgt cgccaatgtg
    31141 caccgcattg aagaaccaca atgggaaaat tatgaccgtg tggtcattgg tgcttctatt
    31201 cgctatggtc actaccactc tgcgttccag gaatttgtca aaaaacatgc gacgcgactg
    31261 aattcgatgc cgagcgcctt ttattccgta aacctggtgg cgcgcaaacc ggagaagcgt
    31321 actccacaga ccaacagcta cgcgcggaag tttctgatga actcgcaatg gcgtcccgat
    31381 cactgcgcgg tcattgccgg ggcgctgcgt tacccacgtt atcgctggta cgatcgtttt
    31441 atgatcaagc taattatgaa gatgtcaggc ggtgaaacgg atacgcgcaa agaagttgtc
    31501 tataccgatt gggagcaggt ggcgaatttc gcccgagaaa tcgcccattt aaccgacaaa
    31561 ccgacgctga aataagcata aagaataaaa aatgcgcggt cagaaaatta ttttaaattt
    31621 cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    31681 aacacgccgc cgggtcagcg gggttctcct gagaatctca acagagaaaa gtaa
//
LOCUS       DAFAVF010000042        31709 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFAVF010000042 DAFAVF010000000
VERSION     DAFAVF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.042"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(476..565)
                     /locus_tag="LGK42_001876"
     tRNA            complement(476..565)
                     /locus_tag="LGK42_001876"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(529..531),aa:Ser,seq:cga)
     gene            659..1456
                     /gene="mtfA"
                     /locus_tag="LGK42_001877"
     CDS             659..1456
                     /gene="mtfA"
                     /locus_tag="LGK42_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBK2658132.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1557..1632
                     /locus_tag="LGK42_001878"
     tRNA            1557..1632
                     /locus_tag="LGK42_001878"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Asn,seq:gtt)
     gene            1794..2709
                     /locus_tag="LGK42_001879"
                     /pseudo
     CDS             1794..2709
                     /locus_tag="LGK42_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2903..4207)
                     /gene="ybtS"
                     /locus_tag="LGK42_001880"
     CDS             complement(2903..4207)
                     /gene="ybtS"
                     /locus_tag="LGK42_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HBK2658133.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4235..5515)
                     /gene="ybtX"
                     /locus_tag="LGK42_001881"
     CDS             complement(4235..5515)
                     /gene="ybtX"
                     /locus_tag="LGK42_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HBK2658134.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5508..7310)
                     /gene="ybtQ"
                     /locus_tag="LGK42_001882"
     CDS             complement(5508..7310)
                     /gene="ybtQ"
                     /locus_tag="LGK42_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HBK2658135.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGYALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(7297..9009)
                     /gene="ybtP"
                     /locus_tag="LGK42_001883"
     CDS             complement(7297..9009)
                     /gene="ybtP"
                     /locus_tag="LGK42_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HBK2658136.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVILVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            9266..10225
                     /gene="ybtA"
                     /locus_tag="LGK42_001884"
     CDS             9266..10225
                     /gene="ybtA"
                     /locus_tag="LGK42_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HBK2658137.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10416..16523
                     /gene="irp2"
                     /locus_tag="LGK42_001885"
     CDS             10416..16523
                     /gene="irp2"
                     /locus_tag="LGK42_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HBK2658138.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDIRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSLEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            16611..26102
                     /gene="irp1"
                     /locus_tag="LGK42_001886"
     CDS             16611..26102
                     /gene="irp1"
                     /locus_tag="LGK42_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HBK2658139.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWLAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSSQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRLATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26099..27199
                     /gene="ybtU"
                     /locus_tag="LGK42_001887"
     CDS             26099..27199
                     /gene="ybtU"
                     /locus_tag="LGK42_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HBK2658140.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAVAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27196..27999
                     /gene="ybtT"
                     /locus_tag="LGK42_001888"
     CDS             27196..27999
                     /gene="ybtT"
                     /locus_tag="LGK42_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HBK2658141.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            28003..29580
                     /gene="ybtE"
                     /locus_tag="LGK42_001889"
     CDS             28003..29580
                     /gene="ybtE"
                     /locus_tag="LGK42_001889"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HBK2658142.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            29711..>31709
                     /gene="fyuA"
                     /locus_tag="LGK42_001890"
     CDS             29711..>31709
                     /gene="fyuA"
                     /locus_tag="LGK42_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HBK2658143.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGI"
BASE COUNT         6790 a         9542 c         8798 g         6579 t
ORIGIN      
        1 aaatgtttcc aggagcaagt catctgtcat ggtaaagcta accagaaata taggttaatt
       61 ttggtgttta aacctttttt acttgttagt atgatacaaa ggctttcaaa aaagctggga
      121 aattcaacaa ctcttggaaa ttatggactt tgtcccgcgg ctttaatctg acggccgcgt
      181 tccttttttg actcaattat tgaccccttc tctacgcaac ttcagttccc gccagcaact
      241 ttgcgacagt ttcataggat caatgtctaa aagaataatg gtgaccgata agaaaacgac
      301 tgaataactg tagattttcg ctcgaaacct tcctgtcagc tccatagcga atcaagtgct
      361 gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac ccttcaagaa tattctacga
      421 ttgttctgtt taggaaaagc aaggcgggaa gtcgggagat aagtcattga taaagtggcg
      481 gagagagggg gatttgaacc cccggtagag ttgcccctac tccggttttc gagaccggtc
      541 cgttcagccg ctccggcatc tctccgttca gatggttgcc atgatgccag gaaatttggc
      601 attttaacag tccctgtccg tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat
      661 gattaagtgg ccctggaaag tacaagaatc agcacatcaa actgcccttc cctggcagga
      721 agcactatcg atcccccttt taacgggtct gacggaacag gaacaaagca aattagtcac
      781 tcttgccgaa cgttttttac agcaaaagcg gcttgttcct ttacagggct ttgaactgga
      841 ttcattaaga agctgccgga tagcacttct attttgccta cccgttctgg agttaggact
      901 ggaatggctg gatggttttc atgaagtctt aatttatcct gcgccatttg tggtcgatga
      961 tgaatgggaa gacgatatcg gtctggtgca taaccaacgt attgttcagt caggtcagag
     1021 ctggcagcaa gggcctatcg ttttgaactg gttggatata caagattctt ttgatgcatc
     1081 tggttttaac ctgattattc atgaagtcgc tcataagctg gacacccgta acggcgatcg
     1141 cgccagcgga gttcccttta tttcgttgcg tgaggttgct ggctgggaac acgatcttca
     1201 tgctgcaatg aacaacattc aggaagaaat cgaattagtt ggtgagaatg cagcgagcat
     1261 tgatgcttat gctgccagtg atcctgctga atgttttgcc gtactttctg aatatttctt
     1321 tagcgcccca gaactttttg ctcctcgttt cccttcattg tggcaacgtt tctgtcaatt
     1381 ttatcaacaa gatcctttgc agagactgca tcacgctaat gatacagact cgttttcggc
     1441 gacgaatgtt cattaattaa caactttgca gattaattaa ccaattgaaa tggcttatga
     1501 aatttagtgt tgacagacaa ggtaccgcta agtaatatgc gctccgttca cacgattcct
     1561 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     1621 tcagaggagc caattttctg ttttcatgca ttcttgcaaa tctttatctg atgttgattc
     1681 aacaggttag tgtgaaaact cttctcggtg cattttgatt ttaccctacg catcgggaaa
     1741 aattggtggt caaatctggg atcaggttag ttcgataatg gagtgacccc catatgttcc
     1801 ttaccgacgc aaaaatccgc accctcaagc cttctgataa accctttaaa gtctccgatt
     1861 ctcacggtct gtatctgctg gtcaagccgg gtggctcccg ccactggtat ctcaaagctg
     1921 gaggttgcgt cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag
     1981 gagttaatcg atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga
     2041 cggcactatc ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg tattggggca
     2101 tatcatcagg gccgtgaact gacccggcat gccgttctgc tgatgctgca tgtgttcatt
     2161 cgctccagtg aactgcgttt cgcccgctgg tcagagattg atttcacaaa ccgagtctgg
     2221 acgatacccg cgacgcgaga acccattatt ggcgtgcgtt attccggccg cggggcaaaa
     2281 atgcgaatgc cgcatatcgt ccccctctca gaacagtcca tcgccattct gaaacagatt
     2341 aaggatatca ccggtaataa tgaactgatc ttccccggcg accataaccc gtataagcca
     2401 atgtgtgaaa acacggtcaa taaggcactg cgggtgatgg gttacgacac gaaaaaggat
     2461 atctgcggtc acggcttccg ggcaatggca tgcagtgcgc tgatggaatc gggtttatgg
     2521 gcaaaggacg cagtagaacg ccagatgagt catcaggagc gcaataccgt gcgcatggct
     2581 tatattcata aggcagagca cctagaagcc cgcaaagcga tgatgcagtg gtggtcggat
     2641 tatctggaag catgccgaga atcttatgca ccgccttata caattggtaa aaataagttt
     2701 atcccatagt atactaacga atccctcact ctggcaaatt accaccacct ccacaggagg
     2761 tggttctata tagactttta tcaacttcat gacgaaaaac acctatttta tcccaaaaga
     2821 agacaataga gtcacagagg aatcaaagag cgactcaatt gtctttggac agtctggttg
     2881 tgaggttaga aaacagttac tcctacacca ttaaataggg cgcaatgctc gctaatttct
     2941 cccgggtttc tgtcagttcg cgttccggcg tagattgcgc gatgattccc gcccccgcct
     3001 gtatccagca gcgctggcta tcctgaaata cggaacgcag aactagcgcc gcatcaaagc
     3061 gcgtatcgtc cagcagcagg attgcgccgg aataaagctc tcgcggcgtc ttctcaattt
     3121 gcataatcgc gttcagagca gcatttttag ggatacctga ggcggtaatc gacggaaaca
     3181 gcacggtaaa cgcatcccag gcatccttgt tttccgaaag ctgaccgctc acgccagacc
     3241 ccagatgctg aacgctgccg cgctggcgaa ccgacattaa atcttcaacc accacgctgc
     3301 ccggctggca aacagcctcc agttcagcaa tagcttcttt gacagaaagg atatgctcaa
     3361 gcacctcttt actgtcgtgc agcagttctg cctctttcgc cttattatgc tccgggtttc
     3421 ccatgcgatc gcgggtgccc gcaagcggtt cagtgaccac tttattgccc atcactgaca
     3481 tcaccagttc cgggctaaag cccagcgctt cgcgtccttc ctgacggaac ataaacgagc
     3541 gcacaggcgt gtttgcctgc cgcccgtata acagcgtggc gggcatatca atccgcgatg
     3601 gcaggggaat ggcgcgcgag acaatcactt tgacatactc gccacggcgg atctccgcaa
     3661 cggcgcgcgc aacctgttgt ttatatgcct caccattgag ggcggtatcc accgccagtg
     3721 gtgcgttctg cgtcgttgta ccggcctctt ttacccactc gcacaaacgt cggcacccgt
     3781 cggcggagtc cgcataaacg gtgacatttc ccttttcaaa aataagttct tcacggggaa
     3841 cggttaacgt cagcagcggc cactccccgg cgttaaaggc aatcccccgc gcatgcgccg
     3901 caaagttgaa cccaacctgt ccatataccc gacgtccttt cacctgagga tgcgccataa
     3961 aacgacgcgc gcagtcggca atggaatcca ccgcccattt ttgctccccg gcatcatcaa
     4021 taaatgtcgc ctgcgttccg tcggcattaa tgtgcagccg tgcctggcac cctttgccta
     4081 aataccaaca gggttgacgc tcatagacat aacactcttc ttctgcgaac tggcgtaaaa
     4141 cgccagaaat cgtcggtagc cattgttcct ctggtaacgc cagatgtaga aattcactga
     4201 ttttcatccc ttacctctct gtgttattcc cggatcaatc aattgcgctt gtcatcagtt
     4261 ctcttgccga atgcagccgg atataagcaa cccacgccgc cagcaacgtg aatgccccgg
     4321 caaacaggaa gcaggccgca tagccaaaat gttgagcaac aacgccaccg ataacgcctg
     4381 ccagaatggc gattgccgca tcagtacatt gaaagagggt gaagtcgaca ccggcctgca
     4441 aaggcgatga aagcgacatc agcgtggcgt aaagacagac cagcgcgcag gccagcgaaa
     4501 tggactggac aatgaccaga cactggagaa tctgcagcag cagatgaccc ggcgccatca
     4561 tgagcgtcat caccaccgcc agcagcgcga tcccctggac gccataagcc gtcaacagcg
     4621 ctctgccggg tgaggtgtat ttcatcagca atccgccggc cagcgttcct gctatgcccg
     4681 ctgcaatatt gccgccgctg aacaacgcgc ccaatgcgga catgctcaac ccatgatcca
     4741 acaacagcgg tgccagaaga ggcagcacaa accgcatgcc tgaattcagc atcaatacca
     4801 gcagtaagcc caggcgcgcc tgcttcctcc ttagcgcata acctaacgcc ggaacatgca
     4861 ggatagtcgc tgttcgcgtc ggctccgtaa tgcgccacag cgggagtgac agagccataa
     4921 tcagcaccgc catcatcagc atggcgacag gccagccgga tgccgccgac agcatcagga
     4981 acaccccgcc gccgcacatc attcccagat agctgccgcc gacctgcacg ctgtttcccc
     5041 agccgtaacc cgtgcgggtc agttgatcga cacaaaagcc gtcgctggcg atatcaatgg
     5101 tggaggcgac cgtgcctgcc accataaaca gaacaaataa cgcgacgatt tgcgtatcgg
     5161 ggaatccccc ttctcgccta aaccagccaa tcgctgcggc aatagtcagg atcgccgcta
     5221 gcgcgacctg accacgcagg attaacatgc gggaacggcg ttcctggcta ccgggcggaa
     5281 gccgccagcg ctcgatccac ggcgcccaaa taaacttcag cgcccagggc agcataaaca
     5341 gggttgtcgc accggcaagc gccagcgaac cgccagcagc gcgcaccagc gcgggtaagg
     5401 actgcataga cagcgccgat accagactct gcgtcacata gacgccggca atagcaaaaa
     5461 tcacccgccc ggtcgtcaac gtcagcggtt tcacattcga ctgaacatca ctcatgcacc
     5521 cactctccag acgcggaaac cccgtcgtcg cgccacacgc gcgcggccat ttgcgcctgc
     5581 cacagcgcct gataacgccc atgatgtgag agcaattgcg catgagtacc ctgctcaacc
     5641 acctgtccct cttccatcac cagaatgttt ccggccccgg cgatggtgga taaacggtga
     5701 gcgatgataa tcaccgtccg gttgtaaacc aggttatcga tcgctttttg caccgccagc
     5761 tcgctttcaa tatccagcgc ggcagtcggt tcatcgagaa tgacgactgg cgcgtttttc
     5821 aataacgctc tggcaatgga aatccgctgg cgctcgccgc ccgatagctg gccgcccatc
     5881 tctcccattg gcgtcagcca gccttgcgga agacgggaaa taaactcaag gcactgcgcc
     5941 gcgcgggcgg cctcttctac ctcctgccgc gtcgcctgtg ggcgcgcgat acggatattc
     6001 gccagcagcg tgtcatcaaa cagccagaca tcctggaaaa caacagaaat caggctattg
     6061 agctgttccg gcgtcaggcg gcgaatatcg acgccgccaa tagaaatctg cccttgctgc
     6121 ggatcggcat agcgcattaa cagtttggtg accgtagttt tgcctgcgcc ggaggcaccc
     6181 accagcgcgc tcatactggc tgccgggaac gtcaaagaaa catgattaag cgcgtagccg
     6241 tcgccttctt cgtagcgata gctgacgttg tcaaagcgga tatcgtaacg ttccggcatc
     6301 tcgctttgct ctgcgacggg aagcggtgct atcgccataa actgctcaat ccgttgcagg
     6361 gcgctggcga tcagttccac aaccgaggtg tagctgataa acatcgccat cggttctgcg
     6421 aagcgcataa tcatcgcgac ggcggcaatc aaaaaggcga ggttcagagt gcccgtcact
     6481 acccagacga tcccggataa caccaccacc tgtaggccca actccacgac gctggcgatc
     6541 agcatcgttg cgccagcgcc ctgacggtga gtgcgggtct gtaagttttc cagcgcattg
     6601 aaatgcgcca gcagcgcccg gcttttatcg gcatcgctgc cgcaggtgcg caataccatc
     6661 atcccctgag caaattcaac gatatcgccg ctcagacgct ggtgcgcttc ccccagcgtc
     6721 tgcatttgtc gccgcatcgc cgggcggcgc cagtaataaa acggcaccag cagagggaag
     6781 atcagcaaca tcaccagccc cagccgccag tctatccaca atgtcgccag cgacgccgtc
     6841 agcggcgtga caatggtgag cagcaaaata ttggctatcg caataacata attgaggttt
     6901 tcatccacgc tgcccagcag caaggcgttc atttcacccg ccctgccgcg ctggagcttc
     6961 tccagcggca cgcggcgtaa ctgctcgcca agtcgcaggc gcaactcatg ggtagcctgc
     7021 gccagatgac cacggtattc aaagcccagg ccataccagc gtagcaccag cgtcacaatt
     7081 gcggcgacgc tgaaggccat agcccagtta agaagttgcg gcgcgtctcc ccgtaacagc
     7141 gcatcaatga tcggataaag acaggcaaag gcgatcccct gcatgaatgc cgccagcaac
     7201 agcgccagca tactgcgtcg cagcatcctg gcctgactgc caacgctgga gataagctgg
     7261 cgccagatga cgcgccaggc caggttatcc gcaggattat tgtctttcat tttccaccac
     7321 ctcttcctgt gttccacccg gcacccaatg ctgcgcctgc tgatagtgtt gccagagccg
     7381 ttgatacagg ccgccctgcg ccaacagttg cgtatggttc cccatttccc tgagctgtcc
     7441 gtcggaaaac agcaatatca catcggcctg agtcaccatt gagagacgat gcgcgaccat
     7501 gatgaccgtc cggccacgca tggccgccgc gagcgccttg ataagcgccg cttcgttttc
     7561 cgggtcagca aacgccgtcg cctcatcgag caccaggatg gggcgatcct gcaaaagcgc
     7621 ccgggcgata gtaatgcgct gccgctggcc gccggagaga aataccccac gttccccgac
     7681 tcgagtgttg taaccttctg gcagagcgct aataaaatca tgggcctgcg ccactctggc
     7741 cgccgctata accgcctcca gcggagtatc cggcgcgccc agacgaatgt tattggctat
     7801 cgtgtcggca aaaaggaagt tgtcctgaaa cacaaacgag agttgcttca tcagggtgtc
     7861 cgtctgcata tcacgcagat ccacgccgcc aatacggata tggcctttgt ccgggtcggc
     7921 gtaacgcagc agcaagcgcg ccacggtgct ttttccggcg ccgcttggcc cgaccagcgc
     7981 cacaatttgc ccggcaggca catgaaagct cacctcctgc aacgcggcgc cagtacgcgc
     8041 ttgcggataa tgaaagctca cctgctcaaa ggtaatgctc gcctcttgcg gctgctggtc
     8101 agactgcggc agcgataact ccggcatcgc gagaacctga taaatacgct gaatgcttaa
     8161 acgcgtttgc gcgaccaggt tattgagcat catcattggc attacggctt cggccattcc
     8221 gctgcccagc agtaaaacgg ccacccacgc gataaaatcg aaactgccat agtgcaacag
     8281 gccgtatccc gaccagatca ggacaaacag ggtcgggaga ggattcagaa tcgagaagga
     8341 aaaacgcgct gaaaaaccgg ctttgcgata ccaggttttg agcacatcga cccactcttc
     8401 aagggcgcgt tgatagcgca aaaaactggt gctgccgcta tcaaacgttc tcaccacggg
     8461 catggcctgc acgaactcaa taaccgccgc gctaacctgc tcgcgcgcct tatgataacg
     8521 ctgagccata ttttccgaac gctggcgggc gagcacgaga ataaccgatc caaacgccag
     8581 tacccccagc gtcgcgattg ccagccgcca gtccagccag aacaagataa cgattgtcgc
     8641 cagcggcatg atgatcgcgc gggcatagag cggcgtgctg tcggccacaa aaatatgtaa
     8701 cgacttcacg tcatccagca tcactttcgc cactgacccg ctgcccattt gctgtaacac
     8761 gccaggcgga agctgcaatg ctttacgggc taacctgctg cgcagaatgt tctccagatg
     8821 aaaagccgcg taatgagagg tgttgaacgc ctgtaagcgc agaacaaaag ttaagaccac
     8881 gcagccgatc acgccgccca gcggccaggc cggaatagcg tcaggcgtcg cgcgaatgtc
     8941 acgcaggctc cacgccagaa aaaggagcgc ccccaggctg gttaatgccg ccagccccgc
     9001 cagcgccata gctaaaataa ccttcctgcg aaccggtgta atcacctgcc agagaggaaa
     9061 tcgggacagc gattcagtat tggatgattg agatgacatg ggagtaactg aatttcctga
     9121 tgaattttta cggacgccaa aaacgacaat tcagggtggc gttctgagaa ttaatgtgaa
     9181 taataaccat tatcaataac tcatctaccc cattcggttc tatttatacc cgcattggtc
     9241 taagccacag ggagataacc aggtcatgac ggagtcaccg caaacgcaat ctgaaatctc
     9301 tattcaccag ttggtggtcg ggaaacctgc aaacgatggc aatattcctg ctcagtgtga
     9361 actgctgcgc tgttcgttgc aggaaggtat ggatattttg ctctggaggg gccattttgc
     9421 gcggccagag acgttgcagc tacacgacga tctgggcagg attaacttct cgtgcatcct
     9481 ggaaggcaca tcgcgctttg cgattcaggg actccggcgc cacaccgact gggagcttgc
     9541 gcgcaatcgc cattacatta cccatacgcc agactgtcgc ggcagcgcct cctattgtgg
     9601 ccgattcgag agcattaccc tctccttcag ccctgaaacc ctcgcgctgt gggtgccgga
     9661 tattagcgcg gtcattaaga ataagatcga ctcccactgc tgttgccagc agcagcgttg
     9721 taatgctgaa acccacttaa cggctcaggc cttgcgtcac gcgctgatgc gcatgcacgg
     9781 cggcttcagt catgaacaaa agccgtcgac gctatggttg caggggcaaa gtctggtcat
     9841 gctcagtctg gtgctggatg aacatcgcga agacgccagt tgcctctcct gccacttcaa
     9901 cccgatggaa cgccagaaac tgctgcgggc gaaggatctg ctgctggctg atctgacgca
     9961 agcgccgggc gtcgcggagc tggccagaga gtccggtctg agcgttctca aaattaagcg
    10021 aggcttccgc gttctgttca acaacagcgt ctatggcctg tttcaggctg aacgaatgca
    10081 ggaagccaga agacgcctgg ccaacggcaa tacctccgtc atgacggtgg cggccgatct
    10141 gggctatgcc aacgccagcc atttctctgc cgccttccag aaacagtttg gcgtaactcc
    10201 ttcgaccttt aaacgcggga tgtaaccggg gtcgcgcccc cctaaactcc tccctgacag
    10261 aggcccggat accccatccg ggcctctgtt ctttccgcct gttaccaacc cgaaacgggc
    10321 ataaataaac ccgtttcggg tagcatctaa ttgttaataa ttattattct catatgagta
    10381 atgcttttcg gtaagacgtg ccatcaggag gaagaatgat ttctggcgca ccatctcagg
    10441 attcgctgtt accggacaac cgccacgcgg ctgattacca acaattacgc gagcggctca
    10501 tacaggaact gaatttaacg ccgcagcagt tacatgaaga gagcaacctg atccaggccg
    10561 gcctggattc cataagattg atgagatggt tacactggtt tcgtaaaaat ggctaccgcc
    10621 ttacccttcg cgagctgtat gccgccccca cgctggcggc atggaaccag ttaatgctca
    10681 gccggtcgcc ggagaacgcg gaagaagaaa cgccgcccga cgaatcatcc tggccgaaca
    10741 tgaccgaaag tacccccttc ccattgacgc cagtacagca cgcctacctg acgggccgca
    10801 tgccggggca gacgcttggc ggcgtgggtt gccacctgta tcaggagttt gaaggccatt
    10861 gtctgacggc gtcgcagctg gagcaggcca tcacgacctt gctgcaacgc cacccaatgc
    10921 tgcatatcgc ctttcgcccc gacgggcagc aggtctggct accgcaacct tactggaacg
    10981 gcgtcaccgt tcatgattta cgccataacg acgctgaaag ccgccaggcc tatctggacg
    11041 cactgcgcca gcgcctgagc caccgtcttt tacgcgtgga aatcggcgaa acgtttgatt
    11101 ttcagctgac gctcttgccg gacaatcgcc accgcctcca tgtcaatatt gacctgctga
    11161 ttatggatgc ctccagcttt acgcttttct tcgatgagct taacgccctg ctggccggag
    11221 aatcgctgcc ggctatcgac acccgctatg atttccgctc gtatttgctg caccagcaga
    11281 agatcaatca accactgaga gacgtcgcgc gcgcttactg gctggcgaaa gcatcgacgc
    11341 ttccccccgc gcccgtcttg ccgctggcct gcgaacccgc cacgctacgt gaagtccgta
    11401 atacccgacg ccgcatgatt gtcccggcaa cacgctggca cgcctttagc aaccgggccg
    11461 gcgagtatgg cgtgacgccg acaatggcac tggcgacctg tttttctgcc gtgctggctc
    11521 gctggggcgg cctgacgcgt ctgctgctta acatcacctt attcgaccgc cagccgctgc
    11581 aaccggcggt tggcgcgatg cttgccgact tcaccaatat tcttctgctg gataccgcct
    11641 gcgatggcga taccgtcagc aacctggcgc gtaaaaacca gctcacgttt acggaggact
    11701 gggagcatcg ccactggtcc ggcgtcgaat tactccgtga actcaaacgc cagcagcgct
    11761 acccccacgg cgccccggtg gtatttacca gcaatctggg gcgttccctc tacagcagcc
    11821 gcgcagaatc gccgttgggc gagccggaat ggggcatctc gcaaacgccg caggtctgga
    11881 tagatcatct ggcgttcgag catcacggcg aggtctggct acaatgggac agcaacgacg
    11941 cgctgttccc tccggcgtta gtcgaaacat tgttcgacgc ctactgccag ttgattaacc
    12001 aactctgcga tgacgaaagc gcctggcaaa agccgttcgc agatatgatg cccgccagcc
    12061 agcgcgcgat acgcgaacgg gtcaacgcca ccggcgcccc cattcccgaa ggcttgctgc
    12121 atgaaggcat tttccgtatc gctctgcaac agccgcaggc gctggcggta acggacatac
    12181 gttatcagtg gaattatcat gagctgacag actatgcccg ccgttgcgcg ggcaggttaa
    12241 tcgagtgcgg ggttcagccc ggcgataatg tggctatcac gatgtcgaaa ggcgcaggac
    12301 aacttgttgc ggttctggcc gtcctgctgg ccggggcggt ttacgttccg gtttcgctgg
    12361 atcagcctgc cgcacggcgc gagaaaatct acgctgacgc cagcgtccgg ctggtgctca
    12421 tttgtcagca cgacgccagc gccgggtcag acgatattcc cgcccttgcc tggcagcagg
    12481 ccattgaggc ggagccgatc gccaacccgg tagtacgcgc ccccacgcaa ccggcctaca
    12541 ttatctacac ctccggctct accggtacgc cgaaaggggt agtcatttct caccggggag
    12601 cgcttaacac ctgttgcgat atcaataccc gctatcaggt tggcccgcat gacagggtgc
    12661 tggccctctc cgccctacat tttgatttat cggtttacga catttttggc gtactgcgcg
    12721 cgggcggcgc gctggtgatg gtgatggaaa atcaacggcg cgatcctcac gcatggtgtg
    12781 agctgatcca gcgccatcag gtcacgctct ggaacagcgt cccggcgctg ttcgatatgc
    12841 tgctgacctg gtgtgaaggt ttcgccgacg ccacgccgga aaacctgcgc gcagtgatgc
    12901 tttccggcga ctggatcggg cttgacctcc ccgcccgtta tcgggccttc cggccacaag
    12961 gacaatttat cgcgatgggc ggcgccaccg aggcgtctat ctggtctaac gcctgcgaaa
    13021 ttcacgacgt ccccgcccac tggcgctcca tcccttacgg ttttccgcta accaaccaac
    13081 gctaccgggt ggtggatgaa cagggccggg actgccctga ctgggtgccg ggtgaattat
    13141 ggattggcgg cattggggtc gcggaaggct atttcaacga tcccctgcgt agcgagcagc
    13201 aatttttgac gctcccggac gagcgctggt atcgcaccgg cgatctcggc tgctactggc
    13261 cagatggcac aatcgagttc ctcggtcgtc gcgacaagca ggtcaaagtc ggaggatatc
    13321 gcatcgagct gggcgaaatc gaaagcgcgc tcagccagct ggtgggggtg aaacaagcaa
    13381 ccgttctggc gatcggcgaa aaagaaaaaa cgctggcggc atacgttgtt cctcagggcg
    13441 aggctttttg cgttaccgat catcggaacc cggcactgcc gcaggcgtgg cacacgcttg
    13501 cgggaacgtt gccctgttgc gccatctcgc cagagatctc cgcagaacag gtagccgatt
    13561 tccttcagca tcgcctgcta aaactgaagc cgggtcacac cgctggcgcc gatcctctcc
    13621 ccctgatgaa ctcactcgct atccagccgc gctggcaggc cgtggtggaa cgctggttag
    13681 catttctggt gacacaacgg cgactgaagc ccgctgctga aggttatcag gtctgcgctg
    13741 gtgaagaacg cgaggatgag cacccgcact tcagcggaca tgatttaacg ttatcgcaaa
    13801 ttcttcgcgg tgcccgtaac gaactgtcgt tactgaacga cgcgcagtgg tcgccggaaa
    13861 gcctggcctt taaccatccg gccagcgccc cgtatattca ggaactggcg acaatttgcc
    13921 aacagcttgc acagcgctta cagcgcccgg tacgcctgct tgaggtggga acccgcactg
    13981 gccgcgccgc agaatcgctg ttagcacagc tcaacgccgg acagattgag tatgtcgggc
    14041 ttgagcagag ccaggagatg ctgctgagcg cccggcagag gctcgccccc tggcctggcg
    14101 cccgtctgtc cctctggaat gcagacacgc tggcggcgca cgctcactcg gcggacatta
    14161 tctggcttaa taacgccctg catcgtctgc tgccggaaga tcccgggctc cttgcgacat
    14221 tacaacagct tgccgttccc ggcgcgctgc tctacgtgat ggagtttcgc cagttaacgc
    14281 cgtccgccct actcagcacg ctcctgttaa ccaatgggca gccggaggcc ttgctgcata
    14341 acagcgccga ctgggcggca ttatttagcg cggccggctt caactgtcag catggcgatg
    14401 aggtcgcggg gttacaacgc ttcctcgtac aatgtcctga caggcaggtg cgccgcgatc
    14461 cccgtcaact tcaggccgcc ctcgccgggc gtctgccggg gtggatggtg ccgcaacgga
    14521 tcgtcttcct cgacgcctta ccgctgacag ctaacgggaa aattgactac caggcgctga
    14581 agcgtcgtca tacccctgaa gcggaaaacc cggccgaagc ggatttaccc cagggcgaca
    14641 ttgaaaaaca ggttgccgcc ctctggcagc aactcttatc aactggcaat gtcaccagag
    14701 aaaccgactt cttccagcaa ggcggcgata gcctgctggc gacccgtctg accgggcaac
    14761 ttcatcaggc aggttatgaa gcgcaattaa gcgacctgtt taatcatccc cggctggcgg
    14821 attttgccgc cacgctgcgg aaaaccgacg tcccggtcga acaaccattc gtccactccc
    14881 ttgaagatcg ctaccagccc tttgcgctta ccgacgtgca gcaggcttac ctggtggggc
    14941 gtcagccggg ctttgccctg ggcggcgtcg gctcacattt ctttgttgaa tttgaaattg
    15001 ccgatctgga cctcacccgg ctggagacgg tctggaaccg attaatcgcc cgccacgata
    15061 tgctgcgcgc catcgtgcgt gatggacagc aacaggtgct cgaacagacg cccccctggg
    15121 tgatacccgc acacaccctc catacgcctg aagaggcgtt gcgggtgcgc gaaaaactgg
    15181 cgcatcaggt actcaacccc gaagtgtggc cggtattcga tctccaggtc ggatacgtgg
    15241 acgggatgcc tgcccgcctg tggctgtgtc tggataacct gttgcttgac ggtctgagca
    15301 tgcagatcct gctggcggag ctggagcacg gctaccgcta cccgcaacag ctgcttccgc
    15361 cgctgcccgt caccttcagg gattatctgc aacaaccctc gctacagtcg cccaatccag
    15421 attctctggc atggtggcag gcgcagcttg atgatattcc tccggcgcct gcgttgccgc
    15481 tgcgctgctt gcctcaggag gttgaaacac cgcgcttcgc ccgcctgaac ggcgcactgg
    15541 acagcacgcg ctggcatcgg ctgaaaaaac gggcggctga cgcccatctc accccgtcgg
    15601 ccgtactgtt gtcggtgtgg tcaacggttc tctctgcatg gagtgcacag cctgacttca
    15661 cgcttaacct tacgcttttc gacaggcgac cgctgcaccc gcaaatcaac cagattctgg
    15721 gcgatttcac ctcgctgatg ctgctgagct ggcatcccgg cgaaagttgg ctgcacagcg
    15781 cgcagtcact acagcagcgg ctgagccaga acctcaacca ccgcgatgtg tcagccatcc
    15841 gcgtgatgcg tcaactggcg caacggcaaa acgtgccagc cgttccgatg cccgtcgtct
    15901 ttaccagcgc gctgggcttt gagcaggata acttcctcgc ccggcgtaat ctgctcaaac
    15961 cggtctgggg catctcccag acgccgcagg tctggctcga tcaccagatt tatgaatccg
    16021 aaggcgaact gcgctttaac tgggattttg tcgccgcgct gtttcctgcc gggcaggtgg
    16081 agcgccagtt tgaacagtat tgcgcattgc taaaccgaat ggccgaggat gaaagcggct
    16141 ggcaactgcc gctcgccgcg ctggtgcctc ccgttaaaca cgcagggcaa tgcgcagagc
    16201 gctcaccgcg cgtatgccct gagcactctc agccacacat tgcggcggac gagagcaccg
    16261 tcagcctgat ttgcgacgcc ttccgcgagg tggttggcga gtctgtcacg cccgcagaaa
    16321 acttctttga ggcgggcgca acgtcgctga atctggtgca actgcacgtt ttgttacaac
    16381 gtcacgaatt ttccaccctg acgttgcttg acctcttcac ccacccttct cctgctgccc
    16441 tggccgatta tctggccggc gtcgccacgg tggagaaaac acaacgacct cgccctgttc
    16501 gccgtcgtca gcggcggata tagcgcgaag caaactgatt ttccccggaa cgccatcgcg
    16561 aacgcatggc gttccattga ctttgtgaat cttaggaaac gggaccgatt atggataact
    16621 tgcgcttctc ttctgcgccg acagcagatt ccattgatgc atcgatcgct caacactacc
    16681 cggactgcga acctgtcgcg gttatcggct acgcctgcca ttttcctgaa tcgccggatg
    16741 gcgaaacgtt ctggcaaaat ctgctggaag gtcgtgaatg cagccgacgc tttacgcgcg
    16801 aagagcttct ggccgtcggt ctggatgccg ccatcattga cgatcctcat tatgtcaata
    16861 tcggtacggt gttagacaac gccgactgct tcgacgccac cctgtttggc tattcgcgac
    16921 aggaagcgga gtcgatggac ccgcagcagc gcctgttttt gcaggcggtc tggcatgcgc
    16981 tggaacatgc cggttatgcc cccggcgccg tcccccataa gaccggcgtt ttcgcctctt
    17041 cccggatgag tacctacccc ggtcgcgaag cattgaacgt gacagaagtc gcgcaggtaa
    17101 aaggtctgca atctctgatg ggcaatgata aagactatat tgccacccgc gccgcgtaca
    17161 aactcaacct gcacggcccg gcgttatcgg tacagaccgc ctgctccagc tcgctggttg
    17221 ccgtgcatct ggcctgtgaa agcctgcgcg ctggcgaatc cgatatggcg gttgccggcg
    17281 gcgtggcgct ctctttcccc cagcaggcag gctaccgcta ccagcccgga atgattttct
    17341 ctcctgatgg tcactgtcgt ccctttgacg cctcggctga gggcacctgg gccggtaacg
    17401 gtctcggctg cgtggtgctg cgtcgcctga gagacgcgct gctgtcaggc gatccgatta
    17461 tctcggtgat cctctccagc gcggtcaaca acgacggcaa cagaaaggtc ggctataccg
    17521 ccccttccgt cgcagggcaa caggcagtca tcgaagaggc gttaatgctg gcggccatcg
    17581 acgacaggca ggtaggttac attgaaaccc acggcaccgg cacaccgctg ggcgacgcga
    17641 ttgaaattga agcgttacgc aacgtctatg cgcctcgccc gcaggatcag cgctgtgcgc
    17701 tcggttccgt gaaaagtaac atgggccatc tggataccgc ggcgggcatt gccggactgc
    17761 tgaaaaccgt tctggcagtc agtcgcgggc aaattcctcc cttactgaat tttcacaccc
    17821 ccaacccggc gctgaaactt gaagagagcc cctttaccat accggtgtcg gcacaggcat
    17881 ggcaggacga aatgcgctat gcgggcgtct cctcctttgg tattggcggc accaactgcc
    17941 atatgatcgt cgcctcgctg cccgacgcgc tcaacgcgcg cctccccaat acggatagcg
    18001 gcagaaaaag taccgcgctg ctgctcagcg ccgccagcga cagcgcgttg cggcggctgg
    18061 cgacggatta tgccggggcg ctgagagaga atgcggatgc cagctctctg gccttcacag
    18121 ccctgcacgc gcgccgtctc gatctcccct tccgcctggc ggcgccatta aaccgtgaaa
    18181 ccgccgaggc gctcagcgcc tgggctagtg agaaatcggg ggcgctggtt tacagcggcc
    18241 acggcgccag cggcaagcag gtgtggctgt ttaccggcca gggctcgcac tggcgcacta
    18301 tgggtcaaac gatgtaccag cactcaacgg cgtttgccga cacgctggat cgctgttttt
    18361 ccgcctgtag cgaaatgctc acgccgtcac tgcgcgaagc gatgtttaac cccgattcgg
    18421 cgcagctgga caatatggcc tgggcgcagc cggcgattgt cgcgtttgaa atcgcgatgg
    18481 cggcgcactg gcgtgctgaa ggactgaagc cagacttcgc cattgggcat tccgtcggtg
    18541 aatttgccgc tgccgttgtc tgcggacact atacgattga acaggtcatg ccactggttt
    18601 gtcggcgcgg cgcgctaatg cagcagtgcg caagcggcgc gatggtggcg gtatttgcag
    18661 acgaagacac gctgatgccg ctggctcgcc agtttgagct ggatctcgcc gccaacaacg
    18721 gtacgcaaca tacggtattt tccgggccgg aagcccgtct cgcggtattt tgcgccacgc
    18781 tctcgcagca tgacattaac tatcgtcgcc tgagcgtaac cggtgcggcg cactccgctt
    18841 tactggagcc gatactcgat cggttccagg acgcctgcgc gggactgcac gcggagccgg
    18901 ggcaaatacc gattatttcc acgctcaccg ccgacgtcat tgatgagtca acgctcaacc
    18961 aggcggatta ctggcgccga cacatgcgcc agccggtgcg ttttatccag agtattcagg
    19021 tggcgcatca gctcggcgcc cgcgtttttc tggagatggg gcccgatgcc cagttggttg
    19081 cttgcgggca gcgcgaatac cgcgataacg catactggat agccagcgcc cggcgtaaca
    19141 aagaggcgag cgatgtcctc aatcaggccc tgctccagct ttacgctgcc ggcgtcgccc
    19201 taccgtgggc cgacctgctg gcgggcgatg gacaacgtat cgctgcgcca tgttatccgt
    19261 ttgatactga acgttactgg aaagagcgcg tctccccggc ctgcgagcct gccgacgcag
    19321 cgctgtctgc cgggctggag gtggcgagtc gcgccgcgac agcgctcgat ctccctcgcc
    19381 tggaagcgct taaacagtgc gccacgcgac tgcacgccat ctacgtcgat caactggtac
    19441 aacgctgtac cggcgatgcc attgagaacg gcgtggacgc catgaccatc atgcgccgtg
    19501 gacgtctgct gccccgctac cagcagctac tccagcgcct gctgaataac tgcgtggtcg
    19561 acggcgatta ccgctgcacc gacgggcgat acgtccgcgc ccgccccatt gaacatcaac
    19621 agcgggaatc actgctgacg gaacttgccg gttattgtga aggttttcag gctattcccg
    19681 acaccatcgc ccgtgccggc gatcggttat atgaaatgat gagcggcgcg gaagaaccgg
    19741 tggcgattat cttcccgcaa agcgcctccg acggcgtgga agtgctgtat caggaattca
    19801 gctttggccg ctatttcaac caaatcgccg ccggggtatt acgcggcatt gtccagacgc
    19861 gtcagccccg ccagccgttg cgtattcttg aagttggcgg cggaaccggc ggcaccaccg
    19921 cgtggctgct gccggaactc aacggcgttc cggcactgga gtaccatttc accgatatct
    19981 cggcgctgtt cacccgtcgc gcccagcaga aattcgccga ctacgatttt gtgaagtata
    20041 gcgagctgga tctcgaaaaa gaggcgcagt ctcagggttt ccaggcacag tcttacgatc
    20101 ttatcgtggc agcgaacgtg attcacgcca cccgccatat tggccgcacg ctcgataatc
    20161 tgcgccccct gctcaagccg ggcgggcgcc tgctgatgcg cgaaatcacc cagccaatgc
    20221 gtctgtttga cttcgttttc ggcccgctgg ttcttccgct acaggatctc gacgcccgcg
    20281 aaggtgagtt attcctcacc accgctcagt ggcaacaaca gtgccgccac gccggattca
    20341 gcaaagtggc gtggctaccg caggatggca gcccgaacgc cgggatgagc gaacatatca
    20401 ttctcgccac gctgcccggt caggcggtta gcgccgtaac attcaccgcg ccatcagaac
    20461 ccgtgttggg gcaggcgctg acggataacg gtgattatct cgccgactgg tctgattgcg
    20521 caggtcagcc cgaacagttt aacgcccgct ggcaggaggc atggcgtctg ctttcacagc
    20581 gtcatggcga cgctctgcct gtggaaccgc cccccgtcgc cgccccggag tggctgggga
    20641 aggttcgctt aagctggcaa aacgaagcct tttcccgcgg tcagatgcgc gttgaagccc
    20701 gtcatcctgc tggcgagtgg ctgccgctat cgcccgccgc gcctcttcct gcgccgcaaa
    20761 cgcattatca atggcgctgg acgcccctca acgtcgccag cattgaccat ccgcttacct
    20821 ttagcttcag cgccggtacg cttgcgcgca gcgacgagct ggcgcaatac ggcatcattc
    20881 acgatccgca cgcctcttca cgactgatga ttgttgagga gagcgaggat acgctggcct
    20941 tagcggagaa agtgatagca gcgctcaccg ccagcgcagc cggattgatt gtggttactc
    21001 gccgcgcgtg gcgagtcgag gaaaatgaag cactctctgc atcccatcac gcgctatggg
    21061 ccttgcttcg cgtcgcggcc aacgaacagc cggaacggtt gcttgccgcc atcgatctcg
    21121 ccgaaaacac cccgtgggaa acgctgcatc aagggttgag cgcagtctca ctatcacagc
    21181 gctggctcgc cgcacggggt gacacccttt ggcttccttc actgtcgccc aatacgggat
    21241 gcgccgctga attaccggca aacgtgttta ccggcgatag ccgctggcat ctggtgaccg
    21301 gagcgtttgg cggattaggc cgccttgccg tgaactggct cagagaaaaa ggggcgcgac
    21361 gcatcgccct gctggcgccg cgcgtggatg agtcatggct acgcgacgtg gagggcgggc
    21421 agacgcgcgt ctgccgttgt gatgtgggcg atgccgggca actggccacg gttcttgacg
    21481 atctggcggc caacggcggc attgccggag cgattcatgc cgctggcgta ttggctgacg
    21541 cgcccttgca ggagcttgat gaccaccagc tggctgccgt tttcgcggta aaagcgcagg
    21601 cggcaagcca gctgttgcaa accctgcgca accacgacgg acgctatctt attctctact
    21661 cttccgctgc cgccaccctc ggcgcgccgg gtcagagcgc ccatgcgctg gcctgcggct
    21721 acctggacgg gctggcccag cagttttcca cccttgatgc gccgaaaacg ctctctgtcg
    21781 cctggggcgc atggggagaa agcggtcggg cggccacgcc ggaaatgctg gcgacgctcg
    21841 ccagccgagg tatgggcgcg ttaagcgatg ccgaaggctg ctggcacctg gaacaggcgg
    21901 tgatgcgcgg cgccccgtgg cgactggcga tgcgcgtttt taccgacaaa atgcccccgt
    21961 tacaacaggc tctgtttaac atcagcgcca cagaaaaagc cgcaacgccg gtcattcctc
    22021 ctgctgatga caacgccttt aacggcagcc tgagcgatga aacagcggtg atggcatggc
    22081 tgaaaaagcg gattgcggtt cagctaaggc tgagcgatcc ggcgtcactg catccaaacc
    22141 aggatctgtt gcaactcggc atggactcgc tgctcttcct tgaactcagt agcgatattc
    22201 agcactacct gggtgtacgc atcaatgcgg aacgggcgtg gcaggatctg tctcctcatg
    22261 gactcacgca gcttatctgt tctaagccag aggcgacgcc tgccgcttcg cagccggaag
    22321 tgttgcggca cgacgccgac gagcgttatg cgcccttccc tttgacgccc attcagcacg
    22381 cctactggct ggggcgaacc cacctcattg gctatggcgg cgtcgcctgt cacgtcctgt
    22441 ttgagtggga taaacgccac gatgagttcg atctcgccat actggagaaa gcatggaacc
    22501 agctcatcgc acgccacgat atgttgcgta tggtggttga tgccgacggg cagcagcgaa
    22561 tcctggcgac aacgccggag tatcacatcc cgcgtgacga tctgcgcgcg ctttccccgg
    22621 aagaacagcg catcgcgctg gaaaaacggc ggcatgaact gagctatcgc gttttgcctg
    22681 ccgaccagtg gcctcttttt gagctggtgg tcagcgaaat cgacgattgc cattaccgtc
    22741 tgcatatgaa cctcgacctt ttgcagtttg atgtgcagag ttttaaagtc atgatggacg
    22801 acctggcgca ggtctggcgc ggtgaaacgc tggcaccgct cgctattacc ttccgtgatt
    22861 atgtgatggc tgaacaggcg cgccgacaga catcggcatg gcacgatgcc tgggattact
    22921 ggcaggaaaa actgccgcaa ctgcccttag cgccagagct gccggtggtt gagacgcccc
    22981 cggaaacgcc acacttcacc accttcaaat cgacgatcgg caagacagaa tggctggccg
    23041 tgaaacagcg ctggcagcag caaggcgtca caccgtctgc cgcgctgctc acgctgtttg
    23101 ccgccaccct tgagcgctgg agccgtacca caacatttac gctgaacctg acgttcttca
    23161 atcgccagcc gatccatccg caaatcaacc agttgattgg tgattttacc tccgtcacgc
    23221 tggttgattt taacttctca gcgccggtga cgttgcaaga gcagatgcaa cagacccaac
    23281 agcgcctctg gcaaaacatg gcgcacagtg aaatgaacgg tgttgaggtg atccgtgagc
    23341 tgggccgcct gcgcggatca caacgtcaac cgctgatgcc ggtagtgttt accagtatgc
    23401 tggggatgac gctggaaggc atgactatcg atcaggcgat gagccatctg ttcggcgaac
    23461 cctgctatgt attcacgcaa acgccgcagg tctggctgga tcatcaggtc atggagagcg
    23521 acggcgagtt gatgtttagc tggtactgca tggacaacgt gctggaaccc ggcgctgccg
    23581 aggcgatgtt taatgactat tgcgccatcc tgcaagccgt catcgccgcc cctgaaagcc
    23641 tgaagactct cgccagcggc atcgccaggc acattccccg ccgacgctgg ccgctgaacg
    23701 cgcaggcgga ctacgacctg cgggatattg agcaggcgac gctcgaatac cccggcatcc
    23761 ggcaggccag agcggaaata accgaacagg gcgcgttgac gctggatatc gtgatggccg
    23821 acgatccttc gccatcagcg gcgatgcctg atgagcacga acttacccaa ctggcgctgc
    23881 cgttgcctga gcaggcgcag cttgatgagc tggaggcgac ctggcgctgg ctggaggcgc
    23941 gtgcgctaca ggggatcgcg gctacgctaa atcgtcacgg cctgtttacc acgccggaga
    24001 tcgcccatcg ctttagcgca atagtacagg cgctgtcctc gcaagcatct caccagcgtc
    24061 tgctgcgcca gtggctacag tgtctgacgg aaagagagtg gttaatccgc gaaggtgaaa
    24121 gctggcgctg ccgcattccg ctcagcgaga ttcctgagcc tcaggaagcg tgcccgcaaa
    24181 gccaatggag ccaggcgctg gcgcagtatc tggaaacctg catcgcccgg cacgacgccc
    24241 tcttctccgg gcagtgttct ccgctggaat tgctgttcaa cgagcagcat cgcgttaccg
    24301 acgcgctgta tcgcgacaac cccgccagcg cctgtctgaa tcgctatacc gcgcagattg
    24361 ccgccttgtg cagcgcagaa cggattctgg aggttggcgc cggaaccgca gccactaccg
    24421 cgccggtgct gaaggccacg cggaacacgc gacagtcgta ccacttcacg gacgtctccg
    24481 cgcagttcct caatgacgcc agagcccgtt tccatgatga atcgcaggtg tcttatgcct
    24541 tgttcgacat caaccagccg ctggatttca ccgcccaccc ggaggcgggt tacgacctga
    24601 tcgttgccgt caatgtgctc cacgacgcca gccatgtcgt ccagacgttg cgcagattaa
    24661 aactgttgct gaaagccggc ggacgtttgc tgatcgttga agcgacggag cgaaacagcg
    24721 tattccagct ggcgagcgtg ggctttattg agggattaag cggataccgc gatttccgcc
    24781 gccgggatga gaaaccgatg ctcacccgct ccgcatggca ggaggttctc gttcaggccg
    24841 ggtttgcaaa cgagctggcg tggcccgcgc aggaatcgtc gccgctgcgc cagcatctgc
    24901 tggtagcgcg ttcgcctggc gtaaatcgcc cggataaaaa agccgtgagc cgctatttac
    24961 agcagcgctt tggcaccggt ctgcccattt tacagatccg gcaaagagaa gcgttattta
    25021 cgccgctgca tgccccgtct gatgcgccga ctgagccagc caaacccacg ccagttgccg
    25081 gggggaatcc ggcactggaa aaacaggtgg ctgaactctg gcaatcgctg ctgtctcgcc
    25141 ccgtggcaag gcatcacgac tttttcgaac tgggcggcga cagcctgatg gcgacaagga
    25201 tggtcgcgca gctaaaccgg agagggattg ccagggctaa ccttcaggat ctgttcagcc
    25261 attcgacgct gagcgacttc tgcgcccatc tacaggcggc tacgtcagga gaggacaacc
    25321 cgatacccct ttgccagggc gacggtgagg aaaccctgtt tgtcttccac gcttcagacg
    25381 gcgatatcag cgcctggctg ccgctcgcta gcgcgttgaa caggcgcgtt ttcggcctgc
    25441 aagcaaaatc gccgcagcgc cttgccacgc tcgaccagat gatcgatgag tatgtcgggt
    25501 gcatccgtcg tcagcagcct cacggccctt atgtgctggc gggttggtcg tatggcgcgt
    25561 ttcttgcggc gggcgccgca caacgcctgt acgccaaagg cgagcaggtt cggatggtgt
    25621 taatcgatcc cgtgtgccga caggatttct gttgcgaaaa ccgggcggcc ctgctgcgcc
    25681 tgttagccga aggacaaacg cctctggcac tgcccgaaca tttcgaccag cagacgcccg
    25741 acagccagct tgccgacttt atcagcctcg ctaaaacggc cggtatggtg tcgcaaaacc
    25801 tgacgctgca agcggcagaa acgtggctcg acaacatcgc gcatctgctg cgtttactga
    25861 ctgagcatac gccgggcgaa agcgttccgg tcccctgtct catggtgtat gccgccggga
    25921 gacccgcgca ctggacgcca gcagaaaccg agtggcaggg ctggataaac aacgccgacg
    25981 acgctgtgat tgaagccagc cactggcaaa tcatgatgga agccccccac gttcaggctt
    26041 gtgcgcaaca cattacgcgc tggctttgcg caacctcaac gcaaccggag aacacgttat
    26101 gatgccgtcc gcctccccaa aacaacgcgt actgattgtg ggcgccaaat ttggcgaaat
    26161 gtacctgaat gcctttatgc agcccccgga ggggctggaa ctggtcggcc tgctggcgca
    26221 gggaagcgcc cgttcaagag agctggctca tgcgtttggc attccgctgt atacctcgcc
    26281 ggaacagata accaggatgc cggatatcgc ctgtattgtc gtgcgttcga ccgtcgccgg
    26341 aggaaccggt acgcagcttg ccagacactt tctgacgcgc ggcgtacacg tcattcagga
    26401 gcatcccctc cacccggatg acatcagttc cttacaaacg ctggcgcagg aacagggctg
    26461 ctgctattgg gtaaacacgt tttatccgca tacgcgcgcg ggacgcacat ggcttcgcga
    26521 tgcgcagcag ctgcgtcgct gtctggctaa gacgccgcag gtggtccacg ccaccaccag
    26581 ccgacagttg ctctactcca ctctggattt gttgttgctg gctctgggcg ttgatgccgt
    26641 cgccgtggag tgcgacgtgg ttggcagctt tagcgatttt cactgtctga gactcttctg
    26701 gccggaggga gaagcctgcc tgctgcttca gcgctatctc gatcctgacg atccagatat
    26761 gcatagcctt atcatgcacc gcctgctgct gggctggccg gaaggtcatc tttcgctgga
    26821 ggcgagctac ggccccgtca tctggtcatc cagcctgttt gtcgcagacc atcaggagaa
    26881 cgcccacagc ctctaccgca gaccggagat cctgcgcgat ctgccgggtc tgacgcgcag
    26941 cgcggcgccc ctgagctggc gcgactgctg cgaaaccgtc gggccggagg gcgtcagctg
    27001 gttgctacac cagttgcgca gccatctggc gggcgaacat ccaccagcgg cctgccaaag
    27061 cgtacatcag atcgcgttat cacgtttatg gcagcagatt ttacgtaaga ccggcaatgc
    27121 ggagatcaga cgcctgacgc cgccgcacca cgaccggtta gccggtttct acaacgatga
    27181 tgataaggag gcgctgtgac gcaatctgca atgtgcatac cgctgtggcc cgcccggaac
    27241 ggcaatactg cgcatctggt catgtgccct ttcgctggcg gcagcagtag cgcgtttcgc
    27301 cactggcaag ctgagcaact ggctgattgc gcgctttctc tggtgacctg gccggggcgc
    27361 gatcgccttc gccatctgga accgctcaga agcattacac aactggcggc actgctggcg
    27421 aacgagctgg aagcatccgt atcgcctgac acgccgcttt tactcgccgg gcacagcatg
    27481 ggggcgcagg tggcgtttga aacctgccga cttctggagc aacgggggct tgcgccacaa
    27541 ggactgatta tttccgggtg ccatgccccg catctgcatt ctgaacgcca gctcagccat
    27601 cgcgatgatg ccgactttat cgctgagctg atagacattg gcggatgttc tcctgaactg
    27661 cgggaaaacc aggaattaat gtcgctgttt cttcctcttc tgcgcgctga tttttacgcc
    27721 accgagagct atcactacga ctcgcccgac gtctgtccgc cgctgcgcac gcctgcgctg
    27781 ttattgtgcg gcagccacga tcgcgaagcc tcctggcagc aggtcgatgc ctggcgtcag
    27841 tggctgagcc acgttacagg cccggtggtg attgacggcg atcatttcta tcccattcaa
    27901 caagcccggt ccttttttac gcagattgtc cgccattttc cccacgcatt ttctgcaatg
    27961 accgcgttgc aaaaacagcc cagtacttca gaaaggtgac gcatgaattc ttcctttgaa
    28021 tctctgattg aacagtatcc cttacccatt gccgaacagt tgcgccactg ggcggcccgt
    28081 tatgcctcgc gaattgccgt cgttgatgca aaggggtcgt taacctacag cgcgcttgat
    28141 gcacaagttg acgaacttgc cgcaggtctg tcatcactgg gtttgcgttc gggggagcat
    28201 gtaattgtgc agcttcccaa cgacaacgcg tttgttaccc tgctgttcgc cttgttaaga
    28261 ctgggcgtta tccccgtgct ggcgatgccc tcgcaacggg cgctggatat cgacgcgctg
    28321 attgagctgg cgcaacccgt cgcttacgtt attcacgggg aaaaccacgc agagctggcc
    28381 cgacagatgg cgcacaaaca cgcctgcttg cgtcatgttc tggtcgctgg agagaccgtg
    28441 agcgacgatt ttacgccgct cttctccctt cacggtgagc gacaggcatg gccgcagcct
    28501 gatgtttccg ccaccgcgtt gttgttgctc tcaggcggca caaccggcac gccaaaactc
    28561 atcccgcgcc gacatgccga ctatagctat aacttcagcg cttctgctga actgtgcggc
    28621 atcagccaac agagcgtgta tctcgccgtc ctcccggtgg cgcataactt tccgctggcc
    28681 tgccccggta ttctgggaac gcttgcctgc ggcggaaaag tggtgctgac cgacagcgcc
    28741 agctgtgatg aggtgatgcc tttaatcgcg caggaaagag tgactcacgt cgccctggtt
    28801 ccggcgctgg cgcaattatg ggtgcaggcc agggagtggg aagacagcga cctttcgtcg
    28861 ctgcgcgtca ttcaggcagg cggcgcccgg ctcgacccga cgcttgctga gcaggttatc
    28921 gccacctttg actgtaccct gcaacaggtt ttcggtatgg cggaaggcct gctctgtttt
    28981 acccggctgg acgatccgca tgccaccatt ctccacagcc aggggcgccc gttgtcccct
    29041 ctggatgaaa tccgcatcgt tgatcaagac gagaacgacg tcgcgccggg cgaaaccggg
    29101 caattgttaa cgcgcggccc ttataccatt tcgggctatt accgcgcccc tgcccacaac
    29161 gcgcaggcct ttaccgcgca agggttttac cgcacaggcg acaatgtcag gctggatgag
    29221 gtggggaacc tgcacgttga gggacgcata aaagagcaga tcaaccgcgc cggagaaaaa
    29281 atagccgcgg ctgaagtgga atcggcactg ctgcgtttag cggaagtgca agattgcgcg
    29341 gtggtcgccg cgccggacac gctgcttggc gagcggattt gcgcgtttat catcgcgcag
    29401 caggtgccaa ctgactatca gcagttgcgt caacaactga cccgtatggg gctcagcgcg
    29461 tggaaaattc ctgaccaaat cgagtttctg gaccactggc cgctcaccgc cgtcggcaag
    29521 atagacaaaa aacgcctgac ggctctcgcc gtcgaccgtt atcgccattc tgcccaataa
    29581 gcgcaaaccg acccgaaaca ggttgaaata aacccgtttc gggtagcact actattagaa
    29641 atagttatca ttttcaattc accattgtcg gtatttttgg cgtttcgccg tcttacaggg
    29701 actcacaaca atgaaaatga cacggcttta tcctctggcc ttggggggat tattgctccc
    29761 cgccattgct aatgcccaga cttcacagca agacgaaagc acgctggtgg ttaccgccag
    29821 taaacaatct tcccgctcgg catcagccaa caacgtctcg tctactgttg tcagcgcgcc
    29881 ggaattaagc gacgccggcg tcaccgccag cgacaaactc cccagagtct tgcccgggct
    29941 caatattgaa aatagcggca acatgctttt ttcgacgatc tcgctacgcg gcgtctcttc
    30001 agcgcaggac ttctataacc ccgccgtcac cctgtatgtc gatggcgtcc ctcagctttc
    30061 caccaacacc atccaggcgc ttaccgatgt gcaaagcgtg gagttgctgc gaggcccaca
    30121 gggaacgtta tatggcaaaa gcgctcaggg cgggatcatc aacatcgtca cccagcagcc
    30181 ggacagcacg ccgcgcggct atattgaagg cggcgtcagt agccgcgaca gttatcgaag
    30241 taagttcaac ctgagcggcc ccattcagga tggcctgctg tacggcagcg tcaccctgtt
    30301 acgccaggtt gatgacggcg acatgattaa ccccgcgacg ggaagcgatg acttaggcgg
    30361 cacccgcgcc agcataggga atgtgaaact gcgtctggcg ccggacgatc agccctggga
    30421 aatgggcttt gccgcctcac gcgaatgtac ccgcgccacc caggacgcct atgtgggatg
    30481 gaatgatatt aagggccgta agctgtcgat cagcgatggt tcaccagacc cgtacatgcg
    30541 gcgctgcact gacagccaga ccctgagtgg gaaatacacc accgatgact gggttttcaa
    30601 cctgatcagc gcctggcagc agcagcatta ttcgcgcacc ttcccttccg gttcgttaat
    30661 cgtcaatatg cctcagcgct ggaatcagga tgtgcaggag ctgcgcgccg caaccctggg
    30721 cgatgcgcgt accgttgata tggtgtttgg gctgtaccgg cagaacaccc gcgagaagtt
    30781 aaattcagcc tacgacatgc cgacaatgcc ttatttaagc agtaccggct ataccaccgc
    30841 tgaaacgctg gccgcataca gtgacctgac ctggcattta accgatcgtt ttgatatcgg
    30901 cggcggcgtg cgcttctcgc atgataaatc cagtacacaa tatcacggca gcatgctcgg
    30961 caacccgttt ggcgaccagg gtaagagcaa tgacgatcag gtgctcgggc agctatccgc
    31021 aggctatatg ctgaccgatg actggagagt gtatacccgt gtagcccagg gatataaacc
    31081 ttccgggtac aacatcgtgc ctactgcggg tcttgatgcc aaaccgttcg tcgccgagaa
    31141 atccatcaac tatgaacttg gcacccgcta cgaaaccgct gacgtcacgc tgcaagccgc
    31201 gacgttttat acccacacca aagacatgca gctttactct ggcccggtcg ggatgcagac
    31261 attaagcaat gcgggtaaag ccgacgccac cggcgttgag cttgaagcga agtggcggtt
    31321 tgcgccaggc tggtcatggg atatcaatgg caacgtgatc cgttccgaat tcaccaatga
    31381 cagtgagttg tatcacggta accgggtgcc gttcgtacca cgttatggcg cgggaagcag
    31441 cgtgaacggc gtgattgata cgcgctatgg cgcactgatg ccccgactgg cggttaatct
    31501 ggtcgggccg cattatttcg atggcgacaa ccagttgcgg caaggcacct atgccaccct
    31561 ggacagcagc ctgggctggc aggcgactga acggatgaac atttccgtct atgtcgataa
    31621 cctgttcgac cgtcgttacc gtacctatgg ctacatgaac ggcagcagcg ccgtcgcgca
    31681 ggtcaatatg ggtcgcaccg tcggtatca
//
LOCUS       DAFAVF010000043        31014 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFAVF010000043 DAFAVF010000000
VERSION     DAFAVF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.043"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(44..754)
                     /gene="nlpE"
                     /locus_tag="LGK42_001891"
                     /gene_synonym="cutF"
     CDS             complement(44..754)
                     /gene="nlpE"
                     /locus_tag="LGK42_001891"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBK2658144.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(768..1190)
                     /gene="arfB"
                     /locus_tag="LGK42_001892"
     CDS             complement(768..1190)
                     /gene="arfB"
                     /locus_tag="LGK42_001892"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK2658145.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(1187..1732)
                     /locus_tag="LGK42_001893"
     CDS             complement(1187..1732)
                     /locus_tag="LGK42_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBK2658146.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            1898..2098
                     /locus_tag="LGK42_001894"
     CDS             1898..2098
                     /locus_tag="LGK42_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBK2658147.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            2085..2345
                     /gene="rof"
                     /locus_tag="LGK42_001895"
     CDS             2085..2345
                     /gene="rof"
                     /locus_tag="LGK42_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBK2658148.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(2394..3692)
                     /gene="tilS"
                     /locus_tag="LGK42_001896"
     CDS             complement(2394..3692)
                     /gene="tilS"
                     /locus_tag="LGK42_001896"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK2658149.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(3757..4146)
                     /locus_tag="LGK42_001897"
     CDS             complement(3757..4146)
                     /locus_tag="LGK42_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2658150.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(4203..6344)
                     /gene="ldcC"
                     /locus_tag="LGK42_001898"
     CDS             complement(4203..6344)
                     /gene="ldcC"
                     /locus_tag="LGK42_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBK2658151.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(6443..7402)
                     /gene="accA"
                     /locus_tag="LGK42_001899"
     CDS             complement(6443..7402)
                     /gene="accA"
                     /locus_tag="LGK42_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK2658152.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(7415..10897)
                     /gene="dnaE"
                     /locus_tag="LGK42_001900"
     CDS             complement(7415..10897)
                     /gene="dnaE"
                     /locus_tag="LGK42_001900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK2658153.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(10934..11530)
                     /gene="rnhB"
                     /locus_tag="LGK42_001901"
     CDS             complement(10934..11530)
                     /gene="rnhB"
                     /locus_tag="LGK42_001901"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK2658154.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(11527..12675)
                     /gene="lpxB"
                     /locus_tag="LGK42_001902"
     CDS             complement(11527..12675)
                     /gene="lpxB"
                     /locus_tag="LGK42_001902"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBK2658155.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(12675..13463)
                     /gene="lpxA"
                     /locus_tag="LGK42_001903"
     CDS             complement(12675..13463)
                     /gene="lpxA"
                     /locus_tag="LGK42_001903"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBK2658156.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(13467..13922)
                     /gene="fabZ"
                     /locus_tag="LGK42_001904"
     CDS             complement(13467..13922)
                     /gene="fabZ"
                     /locus_tag="LGK42_001904"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK2658157.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(14027..15052)
                     /gene="lpxD"
                     /locus_tag="LGK42_001905"
     CDS             complement(14027..15052)
                     /gene="lpxD"
                     /locus_tag="LGK42_001905"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBK2658158.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(15056..15541)
                     /gene="skp"
                     /locus_tag="LGK42_001906"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(15056..15541)
                     /gene="skp"
                     /locus_tag="LGK42_001906"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBK2658159.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(15663..18095)
                     /gene="bamA"
                     /locus_tag="LGK42_001907"
     CDS             complement(15663..18095)
                     /gene="bamA"
                     /locus_tag="LGK42_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBK2658160.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(18125..19477)
                     /gene="rseP"
                     /locus_tag="LGK42_001908"
     CDS             complement(18125..19477)
                     /gene="rseP"
                     /locus_tag="LGK42_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBK2658161.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(19489..20346)
                     /gene="cdsA"
                     /locus_tag="LGK42_001909"
     CDS             complement(19489..20346)
                     /gene="cdsA"
                     /locus_tag="LGK42_001909"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK2658162.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(20359..21117)
                     /gene="ispU"
                     /locus_tag="LGK42_001910"
                     /gene_synonym="uppS"
     CDS             complement(20359..21117)
                     /gene="ispU"
                     /locus_tag="LGK42_001910"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBK2658163.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(21306..22502)
                     /gene="ispC"
                     /locus_tag="LGK42_001911"
     CDS             complement(21306..22502)
                     /gene="ispC"
                     /locus_tag="LGK42_001911"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBK2658164.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(22594..23151)
                     /gene="frr"
                     /locus_tag="LGK42_001912"
     CDS             complement(22594..23151)
                     /gene="frr"
                     /locus_tag="LGK42_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK2658165.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(23442..24167)
                     /gene="pyrH"
                     /locus_tag="LGK42_001913"
     CDS             complement(23442..24167)
                     /gene="pyrH"
                     /locus_tag="LGK42_001913"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK2658166.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(24314..25165)
                     /gene="tsf"
                     /locus_tag="LGK42_001914"
     CDS             complement(24314..25165)
                     /gene="tsf"
                     /locus_tag="LGK42_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK2658167.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(25423..26148)
                     /gene="rpsB"
                     /locus_tag="LGK42_001915"
     CDS             complement(25423..26148)
                     /gene="rpsB"
                     /locus_tag="LGK42_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK2658168.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            26516..27310
                     /gene="map"
                     /locus_tag="LGK42_001916"
     CDS             26516..27310
                     /gene="map"
                     /locus_tag="LGK42_001916"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK2658169.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            27372..30044
                     /gene="glnD"
                     /locus_tag="LGK42_001917"
     CDS             27372..30044
                     /gene="glnD"
                     /locus_tag="LGK42_001917"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBK2658170.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            30075..30899
                     /gene="dapD"
                     /locus_tag="LGK42_001918"
     CDS             30075..30899
                     /gene="dapD"
                     /locus_tag="LGK42_001918"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBK2658171.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
BASE COUNT         7362 a         8617 c         7558 g         7477 t
ORIGIN      
        1 agcgcatcag gcaattttgc gtttgtttct gccttaagca ggtttactgc cccaaactac
       61 tgcaatcctt attcgggtaa aatttacccg ccgtatcggg cgctaatact ttggttggcg
      121 caccagtatc cggattagcc tcaagcgtaa agtgaccttc tactgacagt aacaccggtt
      181 tttcactgtg accgcgcgca gccaggtagc tacgctccag ctctgcgtta ttcgctacca
      241 tgaaacgttt tccggtcgcg caatcagtga aggtcgccgc atcagccata taaaaataca
      301 tgcctcgcag ggtcatcggc gtcataggta aactagattg tgccggttcc agcgtatagt
      361 tgaactgcga ttcaatcgga ttgccttcac gatcgagcat ctccagcgca tcgcctttcg
      421 cccgataata tgacttttca cctttgctgt cggttaatac cagcttgtca gcggttcgcg
      481 cccatgtacc gtaggaagcg aaggaggaag gttcttcacg agcaccgaga taacgctcat
      541 tcatcaccca tgttccgtct ttttcgagga acagagaggt ttcgattcct tcgcaatcag
      601 cacacggcag cacgccgcgc caactttgcg gcatcggttt cagttcggca gcctgtgctg
      661 gagaaagcgt atcgacttcg gcccggttat tacatcccat cagagtaaag aggctgatta
      721 cagccatcgc tgtcactatc gcttttttca ccatctattc cttcttttta ttcccgaccg
      781 ctgcgtactt tgccgcgcat cgccttcacg cttgattttt gtgctttcga tgccagcctg
      841 cgctctttcg atgcacgggt gggccgcgtg ggtcgtcggg ctttttgttc tgttgttaaa
      901 tctttaatca ctgccaccag ccgggctagc gcggcttcgc ggttcagttc ctgactgcgg
      961 tattcctgtg ccttaatgac aatcacgcca tcactgctga tcaaatgatg gctggcggcg
     1021 agtagacgct ctttgtaata ctctggcagg ctggacgccc gaatgtcaaa acgcagatga
     1081 atggccgttg aggtcttatt aacatgctgc ccgcccgcgc cctgcgcacg aatggcggtg
     1141 atctcaagct caccatcggg aatagcaaca tgtcgggaaa tcacaatcat gaaggttgtt
     1201 gccaggcggt taagttaact tccagattat tcttatcgtc cgataaccag atcacgccat
     1261 cctgaatcgt tgcctgcagc gtcatggtac gatcggcaaa ggcgcttact ttcgccagtt
     1321 gttcatcgtc cagataccag acggaaagat tggcaaactg cacacatttg ctctgatttt
     1381 gctgccacca gatttgcgcc gcccgactat tataggtaaa cagcgccact tctgcggcct
     1441 gggtgcaggc tttcttaatc cgccgctcat ccggcagccc cagctcaatc cacaaatcaa
     1501 tgcccaggtg atcgttacgc agccacgctt ccggctcatc atcggcacac aaaccacggg
     1561 taaattgcag acgttcatca gcatatttca gccacgccag caagcgcagc atcatacgct
     1621 cctgggtttc tgaagggtgg cgcgccagcg tcagagaggc atcgagaaac tggttgcggt
     1681 cgagatcggc cacattaacc gtcgctttat aaattgtcgc tttaagcgcc ataagaaaac
     1741 tcctttcgaa tcaggaggcc attgtaacgg aatacgtgca gttgcggcgc tgatattgca
     1801 gaaatgagtg tggtatagtc accttgcgaa acagagttta tcttcgtagg cttagacttg
     1861 catccacggt taagtcagag tgctgacagg agggcatgtg gaaaaatatt gtgagttaat
     1921 acgcaagcgg tacgcggaaa tcgccagcgg agacttagga tacgttccgg acgcgctggg
     1981 ctgcgttttg aaagtgctga atgaaatggc tgccgacgac gccctttctg aggctgtcag
     2041 ggaaaaagca gcatacgctg cggcaaactt actggtgagc gattatgtca atgaatgata
     2101 cgtatcaacc aatcaattgt gatgattacg ataatcttga gctcgcctgc cagcatcatt
     2161 taatgctgac acttgagctg aaagatggcg aaaaattgca ggcaaaagcc agcgatttag
     2221 tctcccgcaa aaatgtggag tatctggtcg tcgaggccgc tggcgaaacc cgtgagctac
     2281 gtctggataa aattaccagc tttagccacc cggaaatcgg tacggtggtg gtgagcgagt
     2341 cctgattgac tttcgccgga tgcgacgttt gatgcgtctt atccggcttt cacttaacta
     2401 agcgttttct gccagacaaa acttacgcca ttctcacctt cagccacacc ttcttgcgtc
     2461 acaaataccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg cgtggtgtca
     2521 cgtagccacg gcggcacgcc taactcttgc cagattttct tcagcttacg tccgccgttg
     2581 cgcccgacaa tatgcagcaa tcctgccgct ttgaaacgca cgctgaccgc ttcgtctgca
     2641 cgcggagggc gaatatcgcc tcccgcagta agctgtacgc ttcccagccc cgccggtaat
     2701 tccagcggtt gtagccacgt ctgccacggc acaatggttt cactttgccc ggtgacggat
     2761 ttaatccacc acagttgcga ctgatatcgg cggatttcaa acgcgcccaa gcgtaaacag
     2821 ggggaggcat cttcccgcgc cagcgccact tcctgccaga tccgcaccag cgcgtcgcgg
     2881 gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc gcgacgggca
     2941 tcactcatcg ctagcattgg cgcaatctgc agcgtcccct gcgacgtttg gcagtgtgct
     3001 aaatcatccg ccagcagttc atccaacaga ctctcttgtt cagcacaaag tgcggcgctg
     3061 cgggccgttg cttcggcaaa atgcggccaa cgctgctgca ataacggcac tacacgcagg
     3121 cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat ccagcgtaaa
     3181 ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa cgggcggata
     3241 agccgtgttc cggcaaactc cgagacttcc cccatagccg aaagcccggc agggccgcta
     3301 ccgcgtttta gcgccagcag aaaggtttca cattgatcgt cgagatgttg cgcggtgacc
     3361 agcacttcgc cgggcaatag ggtacgggca aatgcctgat aacgcgcctg ccgcgcttgg
     3421 gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag cggcacctgc
     3481 cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc acttaaaccg
     3541 tgatgcacat gaatagcgcg cagagtgacg cccggatttt ccgtccgcca ctgcaccaac
     3601 tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat ctggcgtgag
     3661 gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag tagcaatgcc
     3721 cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca gttccaacgg
     3781 cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat cgacacggat
     3841 ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga tgtcatcaac
     3901 gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct ccggcgggaa
     3961 cggaaaagag aaaagctcaa tcacatattg cccattaagc gccaaatccc ctttccacga
     4021 gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat cgcagtagaa
     4081 agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct gttttaaacc
     4141 cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc cgctctggca
     4201 agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc ctgtttcgcg
     4261 ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag catcagtaga
     4321 aaatcgagta ccgtgcggct ctctttggtc agcatttctc ctggcatcaa cagcggtacg
     4381 cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg ttccagcgca
     4441 atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt catgatcatt
     4501 tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt acgaatcagc
     4561 ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc gggatcttca
     4621 gcatagagat cgggtagcat atttttgatc cgcaggttga gatcgtaaga gcgtttaaat
     4681 tccgtcaacc cacgcaataa tcccattgct ttggttttat cgataccaat actaaagaga
     4741 aacagcaggt tataagggcc ggttttctct actacgatcc cacgttcgtc gaggaatttt
     4801 gccaccagcg ccgccgggat cccctcctca ctcatattgc cctgttcgtc catccccggt
     4861 gtcaaaatag tgactttaac cggatcgaga aacatatgat cggcatccgc atcgttaaag
     4921 ccgtgccact gttcgcctgg cgcaacgggc cagcattcgg cttcatccac ctgcggcggt
     4981 tgccagatat cgaaaaacca accgtcagac tcttcccgca accgctggac ctctttgcga
     5041 aaatgcagcg cccgctccac cgaacggtta atcagccgtt tgcccggatt accacgcagc
     5101 atcgccgccg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt ggtggtatgc
     5161 atcataaagg cttcgttaaa cgcctcttcg tcatactcgc ctttaatgtg gatcagcgaa
     5221 gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat cacttttccc
     5281 gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa atgggtgtac
     5341 ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt gatccagtcg
     5401 gtgttgtaga gcaagccatc ataggtggag ttggtaatca ccgcatgaac cggccattgt
     5461 gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc acggcgcggg
     5521 atcccaccaa gaatccccaa cgcattacgc gtcggtttca gccagactgg cactacatcg
     5581 ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcgatcaa cagcgtactg
     5641 ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc gttggtaacg
     5701 atataactct gttccgcgcc aaaagtccgc gcgatgtact cttccgcttc caagtgtggc
     5761 ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc agccttgagg
     5821 gtattcccgc cgaaaaaatc ataaaacagg cagccaaccg ggcttttttg atatgcggtg
     5881 ccgcccatat gccccggcgt acaaaaggtg tacttccgct ctttaacgta ggtaaacaag
     5941 gctttcgtga acggcggtgt aatgttatca agatattcgt cggtgtactg acgcatacga
     6001 atggcgatat cttccgcctg ccccagcgca tattcaaaaa accagagcgc catccgcata
     6061 tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag cgggagatat
     6121 tcattaagtt gattgatatc gctgcataaa tcgagactgt actcatccca gtcaaaaatc
     6181 acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct gttttgtggc
     6241 cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat gggctcatct
     6301 ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc ctcctggaaa
     6361 atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca ccgaagtgac
     6421 cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc tgataacgac
     6481 gattttttaa atcttcagtg cttaacacat cgagatcggc cagatccgcc agcagttgcg
     6541 ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccacccagc ggttccggga
     6601 tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc atcgcttcag
     6661 ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct tccggcgaga
     6721 taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca atcgccagcg
     6781 caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg aggcgagaca
     6841 tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg cccacgcccg
     6901 gataagcccc cggggtgtcg ataaaggtga tgattggcat cttaaagcgt tcagccattt
     6961 gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag ttacggcgaa
     7021 ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga cgaccatcga
     7081 gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg ccagccagtt
     7141 cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc tgtggatggc
     7201 gtgccagttg cgcaatctgc catgcaccaa gatcggcgaa gattttacgc gtcagttcta
     7261 cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt ttctcatcct
     7321 gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc ggctgttcaa
     7381 aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt tccacctgct
     7441 ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag acacgccacg
     7501 tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga tgtactggaa
     7561 ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc tggtcatcaa
     7621 tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg gcttcgtcaa
     7681 tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca aagctgacct
     7741 gtccgctgac gataagtatg cggtcttttt ccagcaattg ctggtattta tccagggcgt
     7801 cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag ataccgatac
     7861 gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca gccgtgatga
     7921 ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca taacgctcaa
     7981 tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac gtttcacgtt
     8041 ccccatctaa taccacctgc tccggccacg gttggcagct ggcgtaggat tgttcaattt
     8101 gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct tccgctttcg
     8161 cgtgttgatc tgccgctttt aacgcatcac ccagcgagtt catcagcgcc gcgcggtgtg
     8221 gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacccgc cggtttaact
     8281 ttttggtgtc ggtacgggcg cagagatcaa acagttcgcg gaagtagccg cctttattac
     8341 gggcttcgat gatggcctca atcggacctt cacctacccc tttgatggcg ccaataccat
     8401 acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt atatctggtg
     8461 gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc ttctcggtgt
     8521 tgtccatatc ggcggtcatt accgccgcca taaactccgc cggatagtgc gctttcagcc
     8581 ataacgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta aatccgtaac
     8641 ccgcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg attccgttct
     8701 tttctgcacc ttcagcaaat acagaacgct gcttagccat ctcttccggc tttttcttac
     8761 ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc acctgcgcaa
     8821 tctgcatgac ctgttcctga tacaggatga tgccgtaggt tggctccagt accggtttca
     8881 ggctttcatg ctgccactgt acgtccggat aggagatttc ttcgcgacca tgtttacggt
     8941 cgataaagtt atccaccatc cctgattgca acggacctgg gcggaacagc gccaccagtg
     9001 cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc atgccgcgcg
     9061 attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag cttttcttgt
     9121 catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc cgccgcttgt
     9181 tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg aagtcgaact
     9241 tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga tgtttgccct
     9301 cttcatcgca gtaaagcggc gcaaaatcgg taattttggt cggcgcgata accaccccac
     9361 cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg tcgatcagcg
     9421 ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct tcaaacgctt
     9481 tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg acaaagccgt
     9541 acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc attgtaccga
     9601 aggtgatgat ctgcgatacc gcatcacgac cgtacatgtc cgccacgtgt tcgataacct
     9661 gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca cgttccgggt
     9721 taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg attttcagcg
     9781 cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt acgccgttat
     9841 ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag cccatctggt
     9901 tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc ttaagacgtt
     9961 cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt gcacgcttga
    10021 ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag tattcaccaa
    10081 gacgcacggt tacgttacag cgtttggcga tctcaacggt gttggcaagg gcttcgggga
    10141 tgtcggcaaa cagctcacac atctcctctt cgctacgcat atattgctgc ggcgaatagt
    10201 tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg cggatttcgt
    10261 gtgcgtcaaa gtcgctgctg tcgataaagc gcacgtcgtt ggtcgccacg acgggcaaac
    10321 cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcgtcc ggcctgccgg
    10381 tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac gcgacacact
    10441 catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc atgcgtccgc
    10501 cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg atgatcggcc
    10561 cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga ttctgatagc
    10621 cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc aggtcgcact
    10681 ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc gctccgtaga
    10741 acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc atacccaacg
    10801 ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc gagtagtcgc
    10861 tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg tttacttaat
    10921 ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca aagctgcgcc
    10981 gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg gttgggtacc
    11041 ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc atttcggcgt
    11101 cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg ctatcgcctt
    11161 tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca atcaacacat
    11221 attccggcgc aatatgcagc ccagcgacgg cacgctgcat cgccagcatg gtcgcatgaa
    11281 gaatgttcag ctcgtcgatt tcgtggggtt ccgcgcggcc cagactccag ctcaacgctt
    11341 tctctttgat ctcttcatag agcgccagac ggcgtttttc gctcagcttt ttggaatcat
    11401 tcagcccggc aatcgggcgc gccgggtcaa ggatcaccgc agcggtgacg accgcgccaa
    11461 ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc ggataaacaa
    11521 attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg cattgcagcg
    11581 gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt tcccgttcgc
    11641 caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct gcaataattc
    11701 tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag ttttcaccag
    11761 ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca tcgggcattt
    11821 cgccagcata cactccaggg ctgccgtacc cgacgccagt agcgccgcat cgctggcgac
    11881 catcgcctca cggcccatcc catccagcaa atgaactgaa aggtctggcg cgacttcagc
    11941 tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca cgatttcgag
    12001 atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac taagcatttc
    12061 aacttctgcg ccacggctcc ccggtagcaa cgccaggcag tgggcatcgt gagggatccc
    12121 cagcacatca cgggcggcat ttttatctgg atctaacggc atggcatcag ccatggtatg
    12181 accgataaag cggcacggta cattgtattt gtcataaaac gctttttcga aaggcagaaa
    12241 tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc gccacgccca
    12301 gacggatgga ctgacgtaat gaatggtttt gataccctgc tttttgaggt taccttcaag
    12361 agtaatattg aagtcgggcg catcaatacc aacaaaaaca tctggcttca gttcgccaaa
    12421 acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga gcacttcaac
    12481 aatgcccatt accgccagtt cttccatttc gtaccaggct tcgcagcctt cagcctgcat
    12541 tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca gagcgcggat
    12601 taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa tcgttaatgg
    12661 acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa atcggtaaag
    12721 gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac ttcatcgagc
    12781 gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat cgcctcacgg
    12841 ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgttgc gtggttaccc
    12901 tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc aaccatcacg
    12961 tgcgcaccaa tgatgcagaa ctgatggact gcggtcatgc cgccgatgat cgcgaagtcg
    13021 tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg gttacctacc
    13081 gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc caccttcgtc
    13141 aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat gcggttacga
    13201 tcgccgattt ccacacgggt cggttcgcca gcatatttca gatcctggtt aacttcgccg
    13261 atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc attcacgaca
    13321 acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac gatacaaaaa
    13381 ggaccaatgt gtgcgttcgc gccaattgac gcgccctctt ccacaatggc ggttggatgc
    13441 acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca catcatcgtt
    13501 gcttcgcaaa ctactttacc atcgaccaga gcaacccctt taaaacgggt caggccgcgg
    13561 cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac cgggcgcttg
    13621 aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag ttttcctacg
    13681 cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag cacacccggg
    13741 aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga gacatttttt
    13801 actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag taagaacggg
    13861 aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt gttagtagtc
    13921 aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct gccgcaatcg
    13981 taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag tcttgttgat
    14041 taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc atcaccagtg
    14101 cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa tagacgcctg
    14161 gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg tcgcatattt
    14221 ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca attttcaggc
    14281 tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg acgttatgtg
    14341 caatctggca ctggttatca atgatcacgc cattgccaat aatagtgtca tccagcgcgc
    14401 cgcgatcgat ggttgtgcag gcaccgatct ccacgcgatc gccaataatt acgcgaccaa
    14461 tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg tctgcgccta
    14521 ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg taaatggtta
    14581 cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg aagcaaccgg
    14641 caccgataat cacgttatcg cccagttcaa cgccggactc aatcaccgcg ttagcgccaa
    14701 tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt gcaatgttct
    14761 gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc aggtagggat
    14821 tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc atgacaaccg
    14881 cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg aacgtaatgt
    14941 gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg ccatcaccgt
    15001 gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc attacttatt
    15061 taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg taagcaacgg
    15121 cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc acagcagtct
    15181 ggatacgagt aaccagtttg ccgcgttctt cgttggaacg acgtgcgcga tcctgctcaa
    15241 aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt tccagcttag
    15301 tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc agatcggttt
    15361 ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg ttagaaacac
    15421 cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca attttgtcag
    15481 ccgcctgagc agaagttgcc agtgctaaac cgagacctgc agctaataac cactttttca
    15541 caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg tggcccggcg
    15601 atcttatatt gatcgcctaa agtcatcgct acactaccac tacattcctt tgtggagaac
    15661 acttaccagg ttttaccgat gttaaactgg aactgttctg ccttgtctcc atcgtacttt
    15721 ttgaacggct gggcgtagga gaacaccaac ggccccaatg gggacatcca ttgtaatgcg
    15781 atacccgcag acatacggat attgcttgga tcactatagt ccggatatcc agaatattgg
    15841 ctggaatccc agtttgtatc ccaaacggta cccatatccc agaagaagga agtacgaacc
    15901 gagttagcat acttatcgct aataaacggc gtcggggtga tgaactcgag gctggcaacc
    15961 gccatggcgt taccgcctac agcatcatcc gatttacaca ggtctttcgc gccgtcctga
    16021 gtcgcacatt cgtaatcata gtccggatca taattactgg cctgatgcgg gaagtaaact
    16081 gctttcggac caattgtatt ggactggaag ccacgcacgg tgctggaacc accggcatag
    16141 aagttctcgt agaacggcat ctctttgccg cctaaaccat caccataacc ccagcgggta
    16201 cgccccagaa caacccattt gtgatcgtca tcgatcggca cataagtcgc cgtgtctaac
    16261 gtcactttgt agtattcgtt atccgatcca ggaatggtca ctttaccggt caggttgaca
    16321 cgtgaaccat ctgtcgggaa gtaaccacgg tcaagcttgt tataggtcca accatagttg
    16381 aacgtgaagt cgtccgtttt gaagctatta tcctgatcag aggtgctcgg atgttcaccc
    16441 atagagtaca gataacgcca catcgcaacc tgaggctgca tgttggacag ggagttatgt
    16501 acataaccca gacctgcacg cagcgagtta tattcgttga tcgggaagcc caacgtcacg
    16561 tctgtaccat aactcttgtt ggtatagtcg gacaggtcgg cgtcatctgc ctggaagtca
    16621 ttatagaaga gacgaccacc gaggcttaca ccatctacag tgaagtacgg gttggttacc
    16681 gacagttcag cataggtctg gtaatcgttt ttggtcccgt tgataccaac agcataacct
    16741 gtacctaacc agttatcctg ctgcacacca gcctggaagc tcacgccact ttcagtaccg
    16801 taaccaatac caaagttgaa gctaccggtg ttgcgctctt ttaccttgta gacgacatct
    16861 acctggtccg ggctacccgg aacacgttgg gtatcggtat cgacagtttc aaagaagccc
    16921 agacgattca gacgctcctt accctgatcg accagatcgc ttcccagcca tgcaccttcc
    16981 atctgacgca tttcgcgacg caggacggca tctttcgagg tatcgttacc ttcaaaacgg
    17041 atcttacgca cgtagaaacg gttacccgca tcaacgttca cgcgtaattt aacggttttg
    17101 tcggcatcgt taatttcagg catcgactgt acgcgcggat aggcataacc atagcgaccg
    17161 agaagctttt tgatgtcatc ttccatcttg gtcactttgg tgccgttata cagctcaccc
    17221 ggctcgatct tagtcagctg ctcaatttca gcggagtgcc cggcaaggtt gccgctcact
    17281 tcaacgccag aaagcttgta ctgatcgcct tcggtgatgt tcaccgtgac gtaaatacct
    17341 tttttatctg gcgtcagact gacctgggta gagtcgatgt tgaaacgggc ataaccgcga
    17401 tccagatagt agctgcgcag ggtttcaagg tcgcccgcca gtttctgttt ctggtattta
    17461 cgatcgccta ccacgttcca ccacggcact tcgtcacgca gttggaaatg agagatcagt
    17521 tcgtcggtgg tgaaagcatg gttaccaaca atgttaattt gctggatttc agctgacaca
    17581 ccttcctgga acaccagttt taggtcaaca cggttgcgcg gcagcggggt cacgacagct
    17641 tttacgctgg cgctatattt accgacgctg tagtagaagt cttccagacc tttctcgata
    17701 tcggcaatgg tggtgcgatc gagggattcg cccacacgca caccagaagc ctcgaggttt
    17761 tgcttcagca tgtcatcttt caccgatttg ttaccggaga aagtaatgct ggcaatggtc
    17821 ggacgttctt ttacctgaac cagaagggta tcaccatcac gaaggacgcg aacatcctca
    17881 aagttgccgg tagcaaacag agcgcgaatg gtattactga tatcttcatc attaaccgtg
    17941 tcgcctgtgc gcaccggcat actgaggagg gccgcaccaa cggcgacacg ctgaaggcct
    18001 tcgaaatgaa tatctttcac tacgaaccct tcagcaccgt atacggtggc gctgctaaac
    18061 agcagcgacg ctatgagcaa ctttttcatc gccatcgtta ttatgcgttc ttcctaacta
    18121 actctcataa ccgagagaaa tcattgaaaa gtgcaagccc cattaacagc accagcagaa
    18181 tcgagccaat gcgataacaa aagtcttgaa cccgctcgga taccggtccg cccttgatct
    18241 tttcgatcgc aaggaacagc agatgccccc cgtcaagtac gggcaacgga aacaggttaa
    18301 ttatccctaa gttcacgcta ataagcgcaa gaaacggcag gtaataaact accccgagtt
    18361 ccgctgtcat cccagccccc ttggcgatag agatcggccc actgaggttg ttcagtttca
    18421 catcaccggt gatcaatttt cccagcatac tgaccgtcag cttcatcagc tgccacgttt
    18481 tgtccgtggc ttcgacgatg gcgttgaacg gcccatactg gcgtacaact ttatactcat
    18541 ctggcaaagg aatgactttc ggctcaatac cgacaaaacc aatcgcttta ccattacccg
    18601 gtttactctc cgggattaat gtcaaagaca agggacttcc ctgcctttcg atttctaacg
    18661 ctaaggattt acccgggtta tcccggacaa gcatcacaaa ggtcacccac tgcgttaagg
    18721 gctgaccatc gactttaacg atcctgtcgc ctgcttgcaa acctgccttg cttgccgccg
    18781 agtttggctg cacattttcc agtacaggtt caatttgcgg cccacgagga cgaatcccca
    18841 gcgaagatac cggatcttct ttatcaggct caaacgccca gtgacgtaaa tcgagcttta
    18901 catcccgccg ttgatcgctg ccaaatggcg ctactgtaat ggtggtgctt tcatcgccaa
    18961 ttttatcgac caactgcaaa cgcacggcat cccaatcagg cgtttcgata ccatctacgg
    19021 cttttagttc cgtacctggt gcaatttgtg cttccgcagc tatcgaattg gctgctattt
    19081 caccaaccac cggacgtacg ccaggcacac caataataaa aaccagccag taggcaaaga
    19141 tagcaaaaat gaagtttgca accggacctg cggcaataat cgccgctcgt tggccaacag
    19201 atttattatt gaaggcatgg tggcggagtt ccggaacgac cggttctgcg cgctcatcca
    19261 gcattttgac ataaccgccc agcgggatca gggcgataac atattcggtg ccgagcttat
    19321 cagttcgccg ccagagcgcc ttaccaaacc ctattgagaa acgctcaacg cgaacaccac
    19381 aacgccgggc aacccagaaa tgaccaaatt catgcacggt gataagtaca cccagtgcaa
    19441 cgatgaacga agccaaatcc cagagaaaac tcagcatatt accttccgtt aaagcgtcct
    19501 gaataccagt aacaacaagc aagcaaagac cggtaccgca gccgtcaggc tatcaatacg
    19561 atctaaaata ccaccgtgtc ctggaattaa atgaccgctg tccttaattc ctgcttcacg
    19621 cttaaacata ctctcggtca gatcgccgag cactgaggct aacgctgcga caatagagca
    19681 aatgagtaag gtgacgggag cgacgtcgag attcgcccac atgccataac cccatgagat
    19741 taccgctgcg gtcgctagtc cacctataaa gccttgccag gttttacccg gagaaacctt
    19801 cggtgccagc ttatgtttac caaacaattt gccaaacata tatgcgccag agtcagcgcc
    19861 ccataccagg atcatgacat agagcagcca tattgcgcca ctgtaatgat tctcgtcgta
    19921 gtgccaggcc cgtaacgcca gcatgcccca gaagaaggga acaatggtta gcacgccaaa
    19981 aataaggcgc aatgttttag agttacgcca gattgctgcg gaacctgggt aaaacagcac
    20041 cagcaatagc gcgacaatcc accaacccag cgaagcccaa agtgagattt caaccagcgg
    20101 ttgatgaata tttcggtgat attccggcaa cagaaaaagc atcagcgcca acaataaccc
    20161 gcataacacc gccaaccata ctcgctgcga acgagtggta aaaccgctaa gctgtcccca
    20221 ttcccacgct gccagcatgc agaccaccag cgttacgatg gcgaacccca ccggcggcaa
    20281 cagaaacaac gccgcgatga cgacgggtat taacacaaaa gcagatatca ggcgatactt
    20341 cagcaaaagc gacccccatc aggctgtttc atcaccgggc tcggtgccgc cgaaacgacg
    20401 ctctcgatta gcaaaggcat ttaacgcccc ttcaaagtct tgttcatcga aatcgggcca
    20461 gagaacatct gtaaagtaaa gttcggcata ggcaatttgc caaagcaaaa agttactaat
    20521 gcgatgctcc cccccagtcc taattactaa atctacaggg gccagttcat gcatacagac
    20581 atgctggttt agcatctctt catctatctg atctggttgc aggtttcctt gctgcacctt
    20641 ttcagccagt tgtctgactc cctggactat atcccaacgt ccaccgtagt tcgccgcaat
    20701 attcagcgtc agaccggtat tcccggctgt tagcgcttca gatttacgaa tacgttcttg
    20761 caaacgcgag ttaaagcgac tggtatcgcc aataatacgc agacgcacgt tatgtcggtg
    20821 cagacttttt acttcgctat cgagcgccca cacaaacagt tccattaacg cactgacttc
    20881 ctgcgctggt cggttccagt tttcactact aaaggcatac agcgttaacg cctcaatacc
    20941 gttgttggcc gcaaaagaga cagcccggcg gacggatttt gccccggctt tatgcccaaa
    21001 ggcacgaatc ttcccttgct tttttgccca gcgaccattg ccgtccataa tgatcgcaac
    21061 atgacggcag ccatgcgctg gcaatttttc gctaagtggt tgagtagcag acaacatcac
    21121 gcgtttttta ttccctgaca gggtttacgg tactcaggaa tactgaagcc tatacaaaaa
    21181 aaagccgtgt aaaaccacgg catacctgac cttataaagc caactactta cgatcagatg
    21241 gcgcagacta tatcactgaa gccctacgct aacaaatagc gcgactctct gtagccggat
    21301 tatcctcagc ttgcgagacg catcacctct tttctggcga cttcacgcgc gttcgcatca
    21361 acagataaca catcgtccac acattgtggt tcgcgcatat ccattttttc cagtacggat
    21421 aaattcaacg cagcgatatc cgtaaagcgg atttgttgcg caagaaaagc agcaacggtg
    21481 atttcgtttg cggcattcaa tgctgtcgtc gctgcctgac cttgttcgaa cgcctccatc
    21541 gccagtttca ggcatggata acgatcataa tccggtgcgg caaatgtcaa cgcacttagt
    21601 ttgcaaaaat cgagcggctt cacgccagag ttcacgcgat tcggccatgc catggtgtgg
    21661 gcaattggcg tacgcatatc cggttccccc agctgcgcca gaacactgcc gtcctgatag
    21721 cgcaccattg agtgaatcac tgactgcggg tgaatcagca cttccatctg gctggcgctg
    21781 gcgttaaaca gccaacgcgc ttcaatgtat tccagacctt tgttcatcat ggtagccgaa
    21841 tcgacagaaa ttttacgccc catcgaccag ttcggatgac ggcaggcttg atccggcgtc
    21901 attgttgcca aatcgcgcaa tggcgtctca cggaaagggc caccagaccc ggtaagtaaa
    21961 atggacacca cgccattttg ctcaaggtca gcgtatccca gattatgctg gataggttgc
    22021 ggtaaactct gaaaaatggc gttatgttcg ctatcgaccg gtaacaattg cgctttgctc
    22081 tgctttacgg cgtccataaa cagacgtccg caggtaacca gtgattcttt attggccagc
    22141 aaaatggttt tacccgcgcg gatcgcagca agcgtaggta acagcccagc agcgccaaca
    22201 atggctgcca tcacctgatc aacatcctca agcgctgcca tatcgcaagc ggcttgttgc
    22261 ccacttaaga cttcggtgcg gctaccctgt tgctgtagca tcgttttaag aagtttcgca
    22321 ctcgcttcat cgtccattac ggcatagcgg ggagagaatt ccaggcactg ttctaccatg
    22381 cgagtgacat ttttgcctgc caccagcgca actacgcgga agtgttcggg attatggcgc
    22441 accacgtcca gcgtgctgca accaatcgag ccggtcgagc ccagaatggt gagttgcttc
    22501 atgaaacatc cagagttgag acagaataaa aagcaaaacg ccgccagccg atccgcaagg
    22561 atctactgag cggcgttttt gtcgttcaag aaatcagaac tgcatcagtt ctgcttcttt
    22621 gtctgccagc gccgcttcaa ttttcttgat tgcagcatca gtcagtttct gtacatcgtc
    22681 ctgagaacgg cgatcgtcgt cttcgctgat ctctttatct ttcaacagtg ctttcacttt
    22741 gtcgttcgcg tcacgacgca cgttacgtac tgcaacacgc gcttgttctg cttcaccacg
    22801 aacgattttg gtcagatctt tacgacgttc ttccgtcagc ggcggcagcg gaacacggat
    22861 gtcgctaccc gcagagttcg ggttcaggcc aagatcggac gccataatcg ctttttcaac
    22921 ggccggagac attgaacgat caaacacgtt gattttcaga gtacgggaat cttctaccgt
    22981 tacgcttgcc agctgacgca gcggcgtcgg cgtgccgtaa tattccacga caatgccatc
    23041 cagcaggctg ggagaagcac gacccgtgcg tattttgctg atttgggttt tgaacgcttc
    23101 tacgcatttg tccatgcgta cttcagcatc ttttctgata tcgctaatca cgttacgaat
    23161 ccttgaaaac ttgtctcagt cagaccagac agtcacacac agtggaagtg tgctaagtat
    23221 agccctgatt aaacatatta cgggtaatcg tctggattat taggctattt tatttgccat
    23281 tttggccccg ggtagtgctc gccatcctca cgtacttgtg tacgcaccgg ttgctgcgcg
    23341 ctggccgtga ccaaactgcc tgcaacaata acgccttata accagactta cgccccgcga
    23401 tacttatgcg gaatcttacc cttatttatc catcacggga attattccgt gattaaagtc
    23461 ccttcttttt cacccattac cacacggcgc agcgcacccg gtttgttcat gttgaaaaca
    23521 cgaatcggta atttatggtc acgagccagc gtgaaggccg ccaggtccat gactttcagc
    23581 tctttttcca gcacttcgct gtaagtcagt tgctcgtaca tggttgcagt tggatctttc
    23641 gccggatcag cagtaaacac gccgtcaact ttggttgctt tcagcaccac atcggcttca
    23701 atttcgatac cacgcaggca agctgctgag tcggtggtaa agaacgggtt acctgtaccg
    23761 gcggagagga tcaccacacg gttgttgcgc aacaggctga tagcttctgc ccagctgtag
    23821 ctgtcgcaca cgccattcaa tggaatagcg gacatcagac gagcgttcac ataggcgcgg
    23881 tgcagtgcat cacgcattgc caggccgttc attacggtcg ccagcatccc catgtggtcg
    23941 cccacaacgc ggttcatacc cgctttcgcc agaccagcgc cacggaacag gttaccccca
    24001 ccaatcacca caccaacctg aatacccagt tcaaccagtt ctttgatttc ctgagccata
    24061 cgatccagta tgcttgcatc aataccgaag ccttcagtgc cctgcagagc ttcgccactc
    24121 aacttaagca gaatgcgttt atagacgggt tttgcattgg tagccatgtt tctttcctga
    24181 gactgtcaac gattgaaatg aattaatact agcgacattg tacgtcgctt ttcagcgcag
    24241 ccccacaagg gttagctgaa tttacaagat gggcacaaaa agaagccgcc ctcaggcggc
    24301 tcctttttga taattaagac tgcttggaca tcgcagcaac ttctgccgca aagtcagtct
    24361 caactttctc gatgccttca cccacttcga agcggatgaa gccagtcact tcagcgttat
    24421 gctctttcag cagctgacca acagttttgc ttggttccat aacgaacggc tgaccggtca
    24481 gagaaacttc gccggtgaat ttcttcatgc ggccttcaac cattttctct gcgatttctt
    24541 tcggcttacc agactgcatc gcgatatcca gctgtacctg gtattctttt tctaccactt
    24601 cagcggatac gtcttctggt ttgatgaatt ctggcttgct tgcagcaacg tgcatagcga
    24661 tgtgtttaac cagctcttcg tcagcgcctt tagcagcaac cagaacgccg atacgcgcac
    24721 cgtgctgata agaacccaga acgtcgcctt ccagcgcagc aacgcggcga atgttgatgt
    24781 tttcaccaat tttcgctacc agcgcaacac gttcttcttc gaactgtgct ttcagaactt
    24841 caacgtcagt gattttgcca gcaacagctg cgtccagaac tttgtctgcg aacgcctgga
    24901 aaccagcgtc ttttgcaacg aagtcagtct ggcagttaac ttccagaatg atgccgtagt
    24961 tgccgtcgat tttggttttg atcacgccgt cagcagcaac gttgcctgct tttttcgctg
    25021 ctttaatagc accggactta cgcatgtttt cgattgccag ctcgatgtcg ccgttagctt
    25081 cagtcagtgc ttttttgcaa tccatcatgc ctgcgccagt acgctcacgc agctctttta
    25141 ccagggatgc ggtaatttca gccattctaa aatcctcgga gatgtgatct gcccggcatt
    25201 aaaccgcaca gatataaaag tgaaaaaggg ggccatatgc aggcccccta accaaacgtg
    25261 tactacctgg tctataaggg ctctccccct tcatatttcg cgccgcagat gcgttgtctt
    25321 cgctcgctca tcccggtcac ttactgatgt aagctcccgg gaattctcga gcttgccgcc
    25381 tttctgcaac tcgaactatt ttgggggagt tatcaagcct tattactcag cttctacgaa
    25441 gctttcttcc gcctgggaag ccagatcctg agaacggcct tcacgtacgg ttgcagcaac
    25501 agcgcccagg tacagggtca cagcacggat tgcgtcgtcg ttacccggga taacgaagtc
    25561 aacaccgtcc ggatcagagt tggtatcaac gatagcaaat accggaatac ccaggttgtt
    25621 tgcttctttg atagcaatgt gttcgtggtc agcatcgatt acaaacagag cgtccggcag
    25681 accgcccatg tctttgatac cgcccaggct gttttccagt ttctccagct cacgagtgcg
    25741 catcagcgct tctttcttgg tcagcttttc gaaagtaccg tcctgagact gagtttccag
    25801 gtctttcaga cgtttgatgg actgacgaac ggttttccag ttagtcagca taccgcccag
    25861 ccagcgatgg ttcacgaaga actggtcgca gctcagagca gcgtctttca ccgcttcgct
    25921 tgcagcgcgt ttagtaccaa cgaaaaggat tttacctttg cgagaagcaa tcttgttcag
    25981 ttcagccaga gcttcgttga acatcggtac agttttctca aggttgatga tgtgaacttt
    26041 gttacgcgca ccgaagatga acggcttcat tttcgggttc cagtaacggg tctggtgacc
    26101 gaagtgaaca ccagccttga gcatgtcgcg catggaaaca gttgccatga ttaaaacctc
    26161 tatataaaag ttggggttat gcctccacgt atcccatatt accgacccca aagggcaccc
    26221 cggaatatgt gccgatacgt gtgtgttgtt acacaaagtg agattgtcgc ttccgtccag
    26281 tctgtgtata cgaaatggat cggaagtccg gcgcgcttta taccacaaat acgtcatgga
    26341 caccaataat tgttggcgct gtgtacagca tcagatgtcg aattttctat tatagaaaac
    26401 cttcagtggc acgtttggcg aaattcagaa tgattctcaa tttgcccggg tgtgatacca
    26461 ttgacggcac ttacatatat attgtcggta tcaccgacgc tgatggacag aattaatggc
    26521 tatctcaatc aagaccccag aagatatcga aaaaatgcgc gtcgctggcc gactggctgc
    26581 cgaagtgctg gagatgatcg aaccgtatgt taaaccgggc gtcagcaccg gcgagctgga
    26641 tcgcatctgt aatgattaca ttgttaatga acaacacgcg gtttctgcct gcctcggcta
    26701 tcacggctat ccgaaatccg tttgcatctc tattaatgaa gtggtgtgcc acggtatccc
    26761 ggacgatgcg aagctgctga aagatggcga tatcgttaac attgatgtca ccgtaatcaa
    26821 agatggtttc cacggcgata cctcgaaaat gtttatcgtc ggtaagccga ccatcatggg
    26881 cgaacgtctg tgccgcatca cgcaagaaag cctgtacctg gcgctgcgca tggtcaaacc
    26941 aggcattaat ctgcgcgaaa tcggtgcggc gattcagaaa tttgtcgaag cagaaggctt
    27001 ctccgtcgtt cgtgaatatt gcggacacgg tattggtcgc ggcttccatg aagaaccgca
    27061 ggtgctgcac tatgactccc gtgaaaccaa cgtcgtactg aaacctggga tgacgttcac
    27121 catcgagcca atggtcaacg cgggtaaaaa agagatccgc accatgaaag atggctggac
    27181 ggtaaaaacc aaagatcgca gcttgtctgc acaatatgag catactattg tggtgactga
    27241 taacggctgc gaaattttga cgctacgcaa ggatgacacc atcccggcga taatctcgca
    27301 cgacgaataa gatgaagccg gcgaatgccg gcttttttaa tgcgataatt taatcttatg
    27361 ggtggcgcac aatgaatacc cttccagaac agtacgcaaa caccgctctc cccaccctgc
    27421 ccggtcaacc gcaaaatcca tgcgtctggc cccgtgatga attaaccgtc ggtgggataa
    27481 aagcccatat cgatactttc cagcgttggc tgggtgatgc ctttgacaat gggatctctg
    27541 cagaacagtt gattgaggcg cgcaccgagt ttatcgacca gctcctgcaa cgattatgga
    27601 ttgaagcggg attcagccag attgccgacc tggcattggt cgccgtcggt ggctacggtc
    27661 gtggcgagct gcatccactt tctgacatcg atttactgat tttaagccgt aaaaagctcc
    27721 cggacgatca ggcgcaaaaa gtgggcgagc tgttaacgct gctctgggat gtaaagctgg
    27781 aagtcggtca tagtgtgcgc acgcttgaag agtgcatgct ggaagggtta tcggatttaa
    27841 ccgtcgccac caatttaatc gaatcccgct tattaattgg cgatgttgcg ctcttcctcg
    27901 aactgcaaaa acatattttt agcgaaggat tctggccttc cgacaagttc tacgcggcga
    27961 aagttgaaga acagaaccag cgccatcagc gttaccatgg caccagctac aaccttgaac
    28021 cagacatcaa aagtagccct ggcggcttgc gcgatattca cactctgcaa tgggtggccc
    28081 gccgtcattt tggcgcaaca tcgctggatg aaatggtcgg gtttggcttc ttaacctcag
    28141 cggagcgggc ggaattaaac gaatgtctgc atatactgtg gcgtattcgc tttgccctgc
    28201 atctggtcgt cagccgttac gataatcgtc tgttattcga tcgccaactt agcgtcgctc
    28261 agcgtctgaa ttacagtggt gaaggcaacg aaccggtcga gcggatgatg aaggattact
    28321 tccgcgttac acgccgcgtc agtgaactca accagatgct gctgcaactg ttcgatgaag
    28381 ccatcctcgc ccttcccgcc gacgaaaaac cacgtccact cgacgatgag tttcagctac
    28441 gcggtacgct aatcgacctg cgtgatgaaa cactatttat gcgccagccg gaagccatct
    28501 tgcgtatgtt ctacaccatg gtgcgcaaca gtgcgatcac cggcatttac tccaccacgc
    28561 tgcgccagtt acgccatgcc cgtcgccatc tgcaacaacc gctgtgtaat attccggaag
    28621 cacgaaaact gtttttgagc attctgcgtc accccggagc ggtgcggcgc gggctattgc
    28681 caatgcatcg ccatagcgtg ctcggcgcgt atatgccgca atggtcgcat atcgtcgggc
    28741 agatgcagtt tgatctgttc cacgcctaca cggtggatga acatactatc cgcgtgatgc
    28801 tgaaactgga gagttttgcc agtgaagaaa cgcgccagcg ccatccgttg tgtgtggacg
    28861 tctggccgcg cctgccgtca actgagctga ttttcatcgc cgcgctgttt cacgatatcg
    28921 ccaaaggacg cggcggcgac cactccattc tcggtgctca ggatgtagtg cattttgcag
    28981 aactccacgg gctgaactca cgcgaaacac agctggttgc ctggctggtt cgccagcact
    29041 tgctgatgtc ggtgacagca caacgccgcg atattcagga ccctgaagtc atcaagcagt
    29101 ttgccgaaga agttcaaacg gaaaatcgtc tgcgctatct ggtatgcctg acagtggctg
    29161 acatttgcgc caccaacgaa acgctgtgga atagctggaa gcaaagtctg ttgcgcgaac
    29221 tctattttgc caccgaaaag cagctacgac gcggaatgca aaacacgccg gatatgcgcg
    29281 aacgggttcg ccatcaccaa ctccaggcac tggcactact gcgcatggat aacatcgacg
    29341 aagaggcgct gcaccaaatt tggtcacgct gtcgtgctaa ctattttgtc cgccatagcc
    29401 caaatcaact ggcctggcat gcccgccatt tattacagca tgatttaagc aaaccgctgg
    29461 tattgcttag cccgcaggct acgcgtggag gcaccgagat ttttatctgg agcccggacc
    29521 gcccctatct gtttgccgcc gtctgtgccg aattagaccg ccgcaattta agtgttcacg
    29581 acgcacaaat tttcaccact cgcgacggta tggcgatgga tacctttatc gtgctggaac
    29641 ccgatggcag cccgctgtcc gcagatcgtc atgaggttat tcggtttggt ctggagcaag
    29701 tactgacgca aagtagctgg cagccaccgc agccccgtcg ccaacccgcc aaattacgcc
    29761 attttactgt tgaaaccgaa gtaacgtttt tgccgaccca taccgaccgc aaatcgttcc
    29821 ttgagcttat cgcgctcgac cagccaggac tgctggcgag agtcgggaaa atttttgccg
    29881 atctgggaat ttcgcttcat ggtgcccgaa ttacaaccat tggcgagcga gtagaagatt
    29941 tattcataat tgccaccgct gaccggcgtg cacttaataa cgagttgcag caggaagtgc
    30001 atcagcggtt gacagaggcc ctcaatccaa acgataaagg gtgatgtgtt tactgatatg
    30061 aaaagagttt aacaatgcag cagttacaga acattattga aaccgctttt gaacgccgtg
    30121 ccgagatcac gccagccaac gcagacaccg ttacccgcga agcggtaaat caggtgatcg
    30181 ccctgctgga ttccggtgca ctgcgtgtag cggaaaaaat tgacggtcag tgggtgacgc
    30241 atcagtggtt gaaaaaagcg gtgctgctct ctttccgtat taatgataat caggtgatcg
    30301 aaggggcaga aagccgctac ttcgacaaag tgccgatgaa attcgccgac tacgacgaag
    30361 cacgtttcca gaaagaaggc ttccgcgttg tgccaccagc ggcggtacgt cagggtgcat
    30421 ttattgcccg taacaccgtg ctgatgccgt cttacgtcaa catcggcgca tatgttgatg
    30481 aaggcacgat ggttgatacc tgggcgaccg tcggttcttg tgcgcagatt ggtaaaaacg
    30541 tccacctttc cggtggcgtg ggcatcggcg gcgtgctgga gccgctgcag gctaacccga
    30601 ccattattga agataactgc ttcattggcg cacgctccga agtggttgaa ggggtgattg
    30661 tcgaagaagg ttccgtcatc tctatgggcg tatacattgg tcagagcacc cgtatttacg
    30721 accgtgaaac cggcgaaatc cactacggtc gcgttccggc ggggtctgtg gttgtttcag
    30781 gtaatctgcc gtcgaaagat ggcaaataca gcctctactg tgcggttatc gttaagaaag
    30841 ttgacgcgaa aactcgcggc aaagtcggca ttaacgaact gctgcgtacc atcgattaag
    30901 agtacatata cgggcagcat gatgctgccc gtatgctttc tggctcagaa taattcgtcc
    30961 tactcattct tcttcccccc ttttttaaat actttttcat cttgtttcgg actg
//
LOCUS       DAFAVF010000044        30867 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFAVF010000044 DAFAVF010000000
VERSION     DAFAVF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.044"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            248..631
                     /gene="secE"
                     /locus_tag="LGK42_001919"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="LGK42_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK2658172.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="LGK42_001920"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="LGK42_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK2658173.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="LGK42_001921"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="LGK42_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK2658174.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="LGK42_001922"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="LGK42_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK2658175.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="LGK42_001923"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="LGK42_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK2658176.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="LGK42_001924"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="LGK42_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK2658177.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="LGK42_001925"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="LGK42_001925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK2658178.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="LGK42_001926"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="LGK42_001926"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK2658179.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="LGK42_001927"
     CDS             12676..13215
                     /locus_tag="LGK42_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HBK2658180.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="LGK42_001928"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="LGK42_001928"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBK2658181.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALIAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="LGK42_001929"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="LGK42_001929"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBK2658182.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVLVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="LGK42_001930"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="LGK42_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBK2658183.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="LGK42_001931"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="LGK42_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBK2658184.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="LGK42_001932"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="LGK42_001932"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBK2658185.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGVSLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="LGK42_001933"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="LGK42_001933"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBK2658186.1"
                     /translation="MSATKLTRREQRAHAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19069..19169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19221..19697)
                     /gene="rsd"
                     /locus_tag="LGK42_001934"
     CDS             complement(19221..19697)
                     /gene="rsd"
                     /locus_tag="LGK42_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBK2658187.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19792..20565
                     /gene="nudC"
                     /locus_tag="LGK42_001935"
     CDS             19792..20565
                     /gene="nudC"
                     /locus_tag="LGK42_001935"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBK2658188.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20605..21669
                     /gene="hemE"
                     /locus_tag="LGK42_001936"
     CDS             20605..21669
                     /gene="hemE"
                     /locus_tag="LGK42_001936"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBK2658189.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21679..22350
                     /gene="nfi"
                     /locus_tag="LGK42_001937"
     CDS             21679..22350
                     /gene="nfi"
                     /locus_tag="LGK42_001937"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBK2658190.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22393..22983
                     /locus_tag="LGK42_001938"
     CDS             22393..22983
                     /locus_tag="LGK42_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBK2658191.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23170..23442
                     /gene="hupA"
                     /locus_tag="LGK42_001939"
     CDS             23170..23442
                     /gene="hupA"
                     /locus_tag="LGK42_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBK2658192.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23455..24150
                     /locus_tag="LGK42_001940"
     CDS             23455..24150
                     /locus_tag="LGK42_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBK2658193.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24152..24577)
                     /gene="zraP"
                     /locus_tag="LGK42_001941"
     CDS             complement(24152..24577)
                     /gene="zraP"
                     /locus_tag="LGK42_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBK2658194.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24815..26191
                     /gene="zraS"
                     /locus_tag="LGK42_001942"
     CDS             24815..26191
                     /gene="zraS"
                     /locus_tag="LGK42_001942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBK2658195.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26188..27513
                     /gene="zraR"
                     /locus_tag="LGK42_001943"
     CDS             26188..27513
                     /gene="zraR"
                     /locus_tag="LGK42_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBK2658196.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27510..28799)
                     /gene="purD"
                     /locus_tag="LGK42_001944"
     CDS             complement(27510..28799)
                     /gene="purD"
                     /locus_tag="LGK42_001944"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK2658197.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28811..30400)
                     /gene="purH"
                     /locus_tag="LGK42_001945"
     CDS             complement(28811..30400)
                     /gene="purH"
                     /locus_tag="LGK42_001945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK2658198.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAESAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7301 a         7996 c         8431 g         7139 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctcgat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt acagcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcgattaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgaa ttgcgaggca tttagcgccc gctctacgtc aacagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaattgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccagcac
    15001 tgtggcctgc tggataataa tctccagcat ggcgcgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcagtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa aatcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgacaccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacaccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 cactgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gttttgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaagtta
    18301 cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac gtatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgaacgccg gggtgcgtgc cagtgatata aatacgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcgtg ggcgcgctgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaagttaagg acgtggcttg
    19021 tcagacgacg gatgaagcaa gagacgatcg ccgcaaaggc gatgcgatag cagattaact
    19081 cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat aaacggtctc
    19141 agccagttaa ggcactccga caagaaatta gccccgcaaa tggggcattg aatgtaaatt
    19201 acgcgttaac agcacagaac tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc
    19261 agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat gtcagataaa
    19321 acctgttgaa actcaaggta attatcatga tcgatagcgg tttccaggct ggaatcgtag
    19381 taatccataa tctgttgagt attggcttca agttgcggcc aaatcttcgc ggcgcgcgcg
    19441 agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa atgcccggca
    19501 gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg
    19561 tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag cagatgctta
    19621 cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta
    19681 tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc tcagtgagaa
    19741 atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc tatggatcgt
    19801 ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa attatggttg
    19861 ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca
    19921 ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca gcggcgtcac
    19981 gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca actggccgga
    20041 cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta ctgcgggcat
    20101 gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg tgagcgttac
    20161 tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc gatcctcctc
    20221 gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt cgtcgaagtg
    20281 ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt
    20341 aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg
    20401 tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg
    20461 aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt
    20521 ctgatagaag atacggtggc gatgtgtcgg gcagagtatg agtgatgata cactgaccgc
    20581 ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg
    20641 ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc gggtcgctat
    20701 ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac
    20761 gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg
    20821 atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct ctattttgaa
    20881 gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt cgataaactg
    20941 ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg taccattcgt
    21001 cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg gacgctggca
    21061 acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat
    21121 gccgatccgc aggcgctgca cgctctactc gataaactgg cgaaaagcgt cactttgtat
    21181 ctgaatgcgc agattaaagc cggtgctcag gctgtgatga ttttcgacac ctggggcggc
    21241 gtgcttaccg ggcgcgatta tcaacagttc tcgctttatt acatgcataa aattgttgat
    21301 ggtttactgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac caaaggcggc
    21361 ggacagtggc tggaagccat ggcagaaacc ggttgcgatg cgctgggcct cgactggaca
    21421 acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg
    21481 gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc gactatactt
    21541 gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat tcatcaggat
    21601 gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc tgaacagtat
    21661 caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct
    21721 tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc
    21781 gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat
    21841 ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt
    21901 ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg
    21961 caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc
    22021 gtcgccagcc attttggttt gatggtggat gtgccaacga tcggcgtggc gaaaaaacgg
    22081 ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc actgatggat
    22141 aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc
    22201 gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa
    22261 ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg
    22321 ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt
    22381 tgagaactca acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg
    22441 cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc
    22501 tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gcattctcga tctcatctgg
    22561 gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa
    22621 gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc
    22681 tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg
    22741 gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt
    22801 cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt
    22861 cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa
    22921 ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat ttttcagcaa
    22981 taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc
    23041 ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga
    23101 gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa
    23161 ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc
    23221 aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa
    23281 gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca ccgcgctgag
    23341 cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg
    23401 gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac
    23461 agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg
    23521 gctggcgctc tgcttctcac tgcctgtagt cataactctt cacttcctcc ctttaccgcc
    23581 agtggatttg ctgaagacca gggcgcagta cgcatctggc gaaaagacag cggcgataat
    23641 gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat
    23701 cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg
    23761 aacattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg tgaatccgat
    23821 ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag
    23881 atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg
    23941 catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat
    24001 tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg
    24061 gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt
    24121 cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc
    24181 caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag
    24241 ccatcgcgat atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct
    24301 ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt
    24361 attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat
    24421 tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc
    24481 acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg
    24541 ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct
    24601 tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg
    24661 catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg
    24721 aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct
    24781 gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga
    24841 ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt
    24901 gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct
    24961 gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg
    25021 gatgcgcatg catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg
    25081 agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg
    25141 catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc ctggagatga
    25201 agaagcgtgg cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca
    25261 gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc agtatgccgc
    25321 gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg
    25381 tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc tggcaagcgt
    25441 gttgtcattc ttctggtatc gccgctatct gcgctcgcgc cagctgttgc aggatgaaat
    25501 gaagcgcaaa gagaagctgg tagctctggg gcatcttgcg gcaggcgttg cccacgaaat
    25561 ccgtaatcca ctttcctcaa ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc
    25621 agggggagaa gcgcatcaat tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg
    25681 cgtggtaagc gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga
    25741 tctcaacacg ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga
    25801 gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag
    25861 gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg
    25921 cgtgattagc gtgacggtca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag
    25981 cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa
    26041 agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg
    26101 tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt
    26161 caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg
    26221 gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg gggctataac
    26281 gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat
    26341 cttgtgcttt gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc
    26401 aaagcgttaa acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg
    26461 gcggtagagg cactgaaaac tggggcgctg gattatctca tcaagccgct ggatttcgat
    26521 aacctacagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca
    26581 cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gctcggcgat gcaacacctg
    26641 ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tactgatcca cggcgattcc
    26701 ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa
    26761 ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc
    26821 ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag
    26881 gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg
    26941 cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc
    27001 tcggttgatg tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc
    27061 gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg
    27121 ctgcggcaac ggcgggaaga tatccctctg ctggctggcc attttctgca gcgctttgcc
    27181 gagcgtaatc gcaaggcggt aaaaggtttt acgccccagg cgatggatct gttgatccac
    27241 tacgactggc cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg
    27301 ctgaccgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccgg tacgccgatc
    27361 ccgctggggc aaagtcagga tattcagccg ctggtagaag tcgaaaaaga agtgattctg
    27421 gcggcactgg agaaaacggg cggcaacaaa accgaggccg cccgtcagtt agggatcacg
    27481 cgcaaaacgc tattggcaaa actgtcgcgt tagttctgct cgcgttcgat agcgcgccag
    27541 ccaatatctt tccggcagaa gcagtcgtcc cagtgaatat cggtcattaa ggcataagcg
    27601 cgtttctgcg cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg
    27661 ctggtcacta cctgctcgtt atccgccagt ttcgtgcccg cgtggaacac tttgccgtca
    27721 gccacttctt ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat
    27781 ccacccgcag ccatcaccac gccgagagaa gcgcgttcat cccactcgga tgttttctcg
    27841 tccagcttcc cttcacaggc tgccaggcag agctcaacca gatcggactt catgcgcagc
    27901 ataatcggct gggtttccgg atcgccaaag cggcagttaa attcgataac cttcggattg
    27961 ccctgtttgt cgatcatcag gcccgcgtag agaaaaccgg tgtaggtgtt gccttccgcc
    28021 gccatgcctt tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg
    28081 gttactaccg gcgcggggga gtaagcgccc atcccgcccg tgtttggtcc ggtatcttta
    28141 tcgcctacgc gtttgtgatc ctggctggta gccatcggca acacatgctc gccgtccacc
    28201 atcacgataa agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc
    28261 gcgtcgccaa aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc
    28321 gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc
    28381 gcgcctttct cacgcagata cgccagcgca ggttctacct cggtgaagtt ctggtattcc
    28441 gccgtaggga tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt
    28501 tgggccgcac ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc
    28561 acgcctttca ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt
    28621 gcgaaatcca acagcgccgg gatatcggtc acgccaatag caacgttttg cagcgcgggt
    28681 tccagtgcag tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg
    28741 gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc
    28801 tattgctcca ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct
    28861 cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg
    28921 tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag
    28981 ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg
    29041 cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat
    29101 agacgatagc attggatttc acgaacttcg ccactttcca gcagaacagc gcatcgcgca
    29161 gttcctgttc ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc
    29221 ccaggtcacg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa
    29281 cacgctcgcc ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt
    29341 tcagggcttc ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga
    29401 tggcctgtgc ggattccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg
    29461 aggttgggtc ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta
    29521 caccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc
    29581 actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct
    29641 gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct
    29701 ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc
    29761 gtgggaagcg accagcggct tctttgcttt caccgtggta cgccggaacc atgctgccga
    29821 agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt
    29881 cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt
    29941 cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca
    30001 ccatggtcgg gccgccgata tcgatgttct caaccgcatc ttccagcgag caaccttcac
    30061 gggccacggt ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct
    30121 gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt
    30181 tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa
    30241 cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca
    30301 gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa
    30361 cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctggatttga
    30421 ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg
    30481 gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt
    30541 ctctttcaat ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac
    30601 attgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa
    30661 tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata
    30721 atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga
    30781 agagaaaaat cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc
    30841 tcgcgacagt gcgctaaagc gcgtcgc
//
LOCUS       DAFAVF010000045        29978 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFAVF010000045 DAFAVF010000000
VERSION     DAFAVF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.045"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="LGK42_001946"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="LGK42_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBK2658199.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="LGK42_001947"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="LGK42_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBK2658200.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="LGK42_001948"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="LGK42_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBK2658201.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="LGK42_001949"
     CDS             complement(1087..1581)
                     /locus_tag="LGK42_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBK2658202.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="LGK42_001950"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="LGK42_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBK2658203.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="LGK42_001951"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="LGK42_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBK2658204.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="LGK42_001952"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="LGK42_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBK2658205.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="LGK42_001953"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="LGK42_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBK2658206.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="LGK42_001954"
     CDS             complement(6745..7032)
                     /locus_tag="LGK42_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBK2658207.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="LGK42_001955"
     CDS             complement(7151..8038)
                     /locus_tag="LGK42_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBK2658208.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="LGK42_001956"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="LGK42_001956"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBK2658209.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="LGK42_001957"
     CDS             complement(9275..9769)
                     /locus_tag="LGK42_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBK2658210.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="LGK42_001958"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="LGK42_001958"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBK2658211.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="LGK42_001959"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="LGK42_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBK2658212.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="LGK42_001960"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="LGK42_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBK2658213.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="LGK42_001961"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="LGK42_001961"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBK2658214.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDSFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="LGK42_001962"
     CDS             13861..14520
                     /locus_tag="LGK42_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2658215.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="LGK42_001963"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="LGK42_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBK2658216.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="LGK42_001964"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="LGK42_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK2658217.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="LGK42_001965"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="LGK42_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBK2658218.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="LGK42_001966"
     CDS             17948..18874
                     /locus_tag="LGK42_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBK2658219.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="LGK42_001967"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="LGK42_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBK2658220.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="LGK42_001968"
     CDS             complement(19225..21444)
                     /locus_tag="LGK42_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBK2658221.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="LGK42_001969"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="LGK42_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBK2658222.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="LGK42_001970"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="LGK42_001970"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBK2658223.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="LGK42_001971"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="LGK42_001971"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK2658224.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="LGK42_001972"
     CDS             complement(26816..27298)
                     /locus_tag="LGK42_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2658225.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="LGK42_001973"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="LGK42_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBK2658226.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="LGK42_001974"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="LGK42_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBK2658227.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="LGK42_001975"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="LGK42_001975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBK2658228.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
BASE COUNT         7339 a         8027 c         7612 g         7000 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg ccccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacaa
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgtta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat agcttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc ggattgtata acattgccac ttttggacaa
    13741 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgattca ggagtacaat aggcaaataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa ctggttaagc
    14341 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 actggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg ggggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagttcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcattac gaatgatctc aatcacttcg tcgatattga gaaacgccac cagcaaacct
    25141 tcgaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggc
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt tttcggttgg
    25681 tcgattaatg cgattgccgc ctgagccact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgggcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttcaga
    26161 ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catgggtacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagtccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaag
//
LOCUS       DAFAVF010000046        29421 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFAVF010000046 DAFAVF010000000
VERSION     DAFAVF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.046"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            92..1750
                     /locus_tag="LGK42_001976"
     CDS             92..1750
                     /locus_tag="LGK42_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBK2658229.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1747..2703)
                     /locus_tag="LGK42_001977"
     CDS             complement(1747..2703)
                     /locus_tag="LGK42_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBK2658230.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2854..3969
                     /gene="macA"
                     /locus_tag="LGK42_001978"
     CDS             2854..3969
                     /gene="macA"
                     /locus_tag="LGK42_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBK2658231.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3966..5912
                     /gene="macB"
                     /locus_tag="LGK42_001979"
     CDS             3966..5912
                     /gene="macB"
                     /locus_tag="LGK42_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBK2658232.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5985..6209)
                     /gene="cspD"
                     /locus_tag="LGK42_001980"
     CDS             complement(5985..6209)
                     /gene="cspD"
                     /locus_tag="LGK42_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBK2658233.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6532..6852
                     /gene="clpS"
                     /locus_tag="LGK42_001981"
     CDS             6532..6852
                     /gene="clpS"
                     /locus_tag="LGK42_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBK2658234.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6883..9159
                     /gene="clpA"
                     /locus_tag="LGK42_001982"
     CDS             6883..9159
                     /gene="clpA"
                     /locus_tag="LGK42_001982"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBK2658235.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9503..9590)
                     /locus_tag="LGK42_001983"
     tRNA            complement(9503..9590)
                     /locus_tag="LGK42_001983"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9554..9556),aa:Ser,seq:gga)
     gene            complement(9844..10062)
                     /gene="infA"
                     /locus_tag="LGK42_001984"
     CDS             complement(9844..10062)
                     /gene="infA"
                     /locus_tag="LGK42_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK2658236.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10347..11051)
                     /gene="aat"
                     /locus_tag="LGK42_001985"
     CDS             complement(10347..11051)
                     /gene="aat"
                     /locus_tag="LGK42_001985"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBK2658237.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11093..12814)
                     /gene="cydC"
                     /locus_tag="LGK42_001986"
     CDS             complement(11093..12814)
                     /gene="cydC"
                     /locus_tag="LGK42_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBK2658238.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12815..14581)
                     /gene="cydD"
                     /locus_tag="LGK42_001987"
     CDS             complement(12815..14581)
                     /gene="cydD"
                     /locus_tag="LGK42_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBK2658239.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14704..15669)
                     /gene="trxB"
                     /locus_tag="LGK42_001988"
     CDS             complement(14704..15669)
                     /gene="trxB"
                     /locus_tag="LGK42_001988"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK2658240.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16214..16708
                     /gene="lrp"
                     /locus_tag="LGK42_001989"
     CDS             16214..16708
                     /gene="lrp"
                     /locus_tag="LGK42_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBK2658241.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16843..20949
                     /gene="ftsK"
                     /locus_tag="LGK42_001990"
     CDS             16843..20949
                     /gene="ftsK"
                     /locus_tag="LGK42_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK2658242.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21104..21715
                     /gene="lolA"
                     /locus_tag="LGK42_001991"
     CDS             21104..21715
                     /gene="lolA"
                     /locus_tag="LGK42_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBK2658243.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21726..23069
                     /gene="rarA"
                     /locus_tag="LGK42_001992"
                     /gene_synonym="mgsA"
     CDS             21726..23069
                     /gene="rarA"
                     /locus_tag="LGK42_001992"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBK2658244.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23160..24452
                     /gene="serS"
                     /locus_tag="LGK42_001993"
     CDS             23160..24452
                     /gene="serS"
                     /locus_tag="LGK42_001993"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK2658245.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24691..27135
                     /gene="dmsA"
                     /locus_tag="LGK42_001994"
     CDS             24691..27135
                     /gene="dmsA"
                     /locus_tag="LGK42_001994"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBK2658246.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27146..27763
                     /gene="dmsB"
                     /locus_tag="LGK42_001995"
     CDS             27146..27763
                     /gene="dmsB"
                     /locus_tag="LGK42_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBK2658247.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27765..28628
                     /gene="dmsC"
                     /locus_tag="LGK42_001996"
     CDS             27765..28628
                     /gene="dmsC"
                     /locus_tag="LGK42_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBK2658248.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28664..29290)
                     /locus_tag="LGK42_001997"
     CDS             complement(28664..29290)
                     /locus_tag="LGK42_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2658249.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
BASE COUNT         7287 a         7292 c         7946 g         6896 t
ORIGIN      
        1 catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt tcccgttata ctgccagcgt
       61 aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg aaattgtggg
      121 ttttcgcggt atcaaccgtt tgtcgttgat gctcgaacaa aacaacgtcc tgattgggga
      181 gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat cgccagaatc
      241 agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata tcaacgggcg
      301 agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt
      361 tcgccgttat cggccgctgg aagcgtgctg gacgccatgc accgatggct atcaccgtat
      421 tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac tgcgcagttt
      481 tctcgataaa gacggacatc cgattgatgt cgaggatatt aacgatcagg cacgccatct
      541 ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa
      601 cggcacggtg ccaaatgttc ccaatgtgga agtcaccgcg cgccagctcg atttcctcgc
      661 ccgtgagttg tcctcacatc cgcaaaatct ctctgatgga cagattcgtc agggactttc
      721 cgcaatggtg cagttgcttg agcattattt ctctgagcag ggggccggac aggcgcgata
      781 tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc tggcgctatc tggacatcat
      841 caaccggatg attgaccgac ctggagggcg ctcgtatcgg gttattttgc tcggcctgtt
      901 tgctacttta ttgcaggcaa aaggcacatt gagactggat aaagacgcgc gtccattgtt
      961 gctgatcgaa gatccagaaa cccgtttaca ccccattatg ctttcagttg cctggcatct
     1021 gttgaatctt ctgccattgc agcgcattgc caccaccaac tcgggtgagt tgctttcgtt
     1081 aacgccggta gagcatgttt gccgactggt acgtgagtcc tcgcgcgttg ccgcctggcg
     1141 tctggggccg agtggcttga gtaccgaaga tagccgacgc atatcctttc acattcgttt
     1201 taaccgtccg tcatcgctgt ttgctcgctg ctggttgctg gtggaagggg aaacggaaac
     1261 ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg aagggatcaa
     1321 ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc gccgaatggg
     1381 gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg ccgctacggt
     1441 acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg cgttaccggc
     1501 gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc accgcgtggc
     1561 gcaaatcccg gaaaatgtac cgatgaatct gcgcaaaatt atctcgaaag cgatccatcg
     1621 ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gccggacgtc gtggtgtgga
     1681 ctccgtaccg acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg
     1741 cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga gcatctcata
     1801 gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt ttcgtgctat
     1861 ctgtgctgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg attcccagaa
     1921 agcgttgtaa tcggcatgga tcttgccttc tttatcacga taacgcaggc tgcggtaaat
     1981 atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg caaacagaca
     2041 ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg caggctttcg tcgcattctg
     2101 gatttcctga tgtggaattt cccattttgc gccttgcagt ccgccaataa acatcgttct
     2161 tttcccctga tattcacaca gggtaaacgt gatctctgcc agaggaatac cttcgctgtt
     2221 gcgaaacagg attgtgctgt caccttcttt atccattgag atcatcatgg tcagctcaag
     2281 cgtgaactgc tcgccgtttt tgccttccag cttcgccagt tgcagcccgg gggtattcaa
     2341 atataagctg aattcttccg ccgacataca tccgcggagt aacgcataat ggtaacgtaa
     2401 cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat gcagacgcac
     2461 aggcagtcgc ggttggcgcg ttaacaacac attgagatta ggccagtggg aaagttcgtt
     2521 catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt tctggcgaaa
     2581 actacggcga tgccagaatt tacccggccg acactgtcca cgtgccagac taagaaaaag
     2641 tgacaggctg ctgagagatt cagatggcgt aaaggtccgt tcagttagct gcgacatatt
     2701 catgaaatca atggttatac atgacgtcga tttcaccatt gcgtatctta accaaacatc
     2761 aatagtgtga ttactaacgt aaattttagg gttttgttga tatttcgttg aagttaatga
     2821 cccggattgg catatggagt attcagaata tttatgaaaa agcggaaaac cgtgaagaag
     2881 cgttacgtta ttgcgctggt gatagtcatc gccggactga ttacgttatg gagaattctt
     2941 aacgcacccg tgccgactta tcagacactg attgtgcgcc ccggtgattt acagcaaagc
     3001 gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg acgtgggcgc gcaggtcagc
     3061 ggtcagttga aaactctgtc ggtggcgatt ggcgataaag taaaaaaaga ccagctttta
     3121 ggggttattg atcctgaaca ggctgaaaac cagattaagg aggtcgaagc aacgctgatg
     3181 gagctacgtg cgcagcggca gcaggcggaa gcggagctga aactggcgcg ggtgacgtat
     3241 tcccgtcagc aacgtctggc acaaacgcag gcggtttcac agcaggatct cgacaccgcc
     3301 gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca aatcaagcgc
     3361 aatcaggcct ctctcgatac ggctaaaacc aatctcgatt acactcgcat cgttgccccg
     3421 atggccgggg aagtcacgca aatcaccact ctgcaaggcc agacggtgat tgccgcacaa
     3481 caagcaccga acattctgac gctggcagat atgagcacca tgctggtaaa agcgcaggtt
     3541 tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag cctggtttac ggtacttggc
     3601 gatccactga cgcgctacga ggggcaaatc aaggatgtac taccgacgcc ggaaaaggtt
     3661 aacgacgcta ttttctatta cgcccgtttt gaagtcccca accccaatgg tttgctgcgg
     3721 ctggatatga ctgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt gctgacgatc
     3781 cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa attgttgcgt
     3841 aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac cgatgttgag
     3901 attgtcaaag ggcttgaagc gggcgatgaa gtggtgattg gtgaggccaa accaggagct
     3961 gcacaatgac gcctttgctc gaattaaagg atattcgtcg cagctatcct gccggtgatg
     4021 agcaggttga ggtgctgaag ggcatcaccc tcgatattta tgccggtgag atggtagcga
     4081 ttgttggcgc ttcaggttcc ggtaaatcga ccctgatgaa tattctcggc tgtctggata
     4141 aggccaccag cggcacctat cgcgtcgccg gtcaggatgt tgccacactg gacgccgatg
     4201 cgctggcgca actgcgccgc gagcatttcg gctttatttt ccagcgttac catttacttt
     4261 cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgctggt cttgagcgga
     4321 aacagcgact gcttcgtgcc caggagttgc tgcaacggct ggggctggaa gaccgtacag
     4381 agtattatcc ggcacagctt tcgggtggtc agcaacagcg cgtcagcatc gcgcgggcat
     4441 tgatgaacgg tggtcaggta attcttgccg atgaaccaac cggcgcactg gacagccatt
     4501 ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga tcgtgggcat acggtgatta
     4561 tcgtcaccca cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa attcgcgacg
     4621 gcgaaattgt gcgcaatcct cccgccattg aaaaagtgaa tgttgctggc gggacggagc
     4681 ctgttgtcaa cacggtgtct ggctggcggc agtttgtcag cggttttaac gaggcgctga
     4741 cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac tttactgacc atgctgggga
     4801 ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt gggtgacgcc gccaaacaaa
     4861 tggtgctggc ggatattcgt tctattggta cgaatactat tgatgtctat cccgggaaag
     4921 attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac ttaatcgcca
     4981 tccaaaaaca accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac ctgcgcctgc
     5041 gttataacaa tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat tattttaatg
     5101 tctatggcat gaccttcagt gaaggaaaca cctttaatca ggagcagctg aacggtcgtg
     5161 cgcaggtcgt ggttctcgac agtaatactc gccgccagct tttcccccat aaagcagatg
     5221 tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt gtggcggaag
     5281 aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctacct tacagcacga
     5341 tgtccgggcg agttatgggc cagtcgtggc tgaactccat tactgtcagg gtaaaagaag
     5401 gatttgacag cgccgaggcg gaacagcaac tcacgcgttt actttcactg cgccacggaa
     5461 agaaggattt ctttacctgg aacatggacg gcgtcttgaa aactgttgaa aagaccacac
     5521 gtactttaca actgtttctg acgctggtgg cggtgatttc gctggtggtg ggcggtattg
     5581 gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt ggcattcgca
     5641 tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt cctgatcgaa gccgtactgg
     5701 tttgcctggt cggtggcgcg ttgggaataa cactgtcact gttaattgct ttcaccttgc
     5761 agcttttctt acccggctgg gagattggtt tttcaccgtt ggcactgctg ctggcgtttc
     5821 tctgctcgac ggtcaccggg attttatttg gatggttacc cgcacgaaat gcggcacgac
     5881 tggatccagt agatgctctg gcacgagagt aatttttgag ataaaaatgc cagccgatcg
     5941 ggctggcatt ttgcctttag gatgtacaca atgagacaga agagctatgc gactgccgct
     6001 tctacttcga cgggcacaat aacactggcg tgattgcctt ttggcccctg gtggacatca
     6061 aactgaacgg attgtccagc ttttagcgtt ctgtaaccat ccatctgaat ggtggaataa
     6121 tgagcgaaaa tatcttcgcc gccgccttca gggcagatga aaccaaaccc tttggcattg
     6181 ttgaaccact taacagtacc cttttccatg cttcgacatc cttcgcaaat cttatacaag
     6241 taagatggaa taaaccgggg tcagagaggg ggctgttcaa aacctcgcca actctagaaa
     6301 tacaatttag agaattaggg cgagccgtca agcgtttgac aggggccagg gggcaggtat
     6361 gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat gtgacagatg tcgctgatgc
     6421 caacgataga tgatagttat ctatcatgtg gagtagattg gtcaggcaaa taagctcttg
     6481 tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac aatgggtaaa
     6541 acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga cgcgctaaaa
     6601 ccgccatcta tgtataaagt gatattagtc aatgatgatt acactccgat ggagtttgtt
     6661 attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca attgatgctc
     6721 gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt tgcagaaacc
     6781 aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct gtgtacgcta
     6841 gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa tcaagaactg
     6901 gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga gtttatgacc
     6961 gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc gctggaagcg
     7021 tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga acaaaccaca
     7081 cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag ttttcagcgt
     7141 gtactgcaac gtgcggtctt ccatgtccaa tcctccggtc gcaatgaggt aaccggtgca
     7201 aacgttctgg tcgctatctt tagcgaacag gagtcgcagg cggcatatct gttgcgtaaa
     7261 catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg taaagacgag
     7321 ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc tggtggggag
     7381 gaacgtatgg agaatttcac gacgaacctg aatcagcttg cgcgcgtggg cggaatcgac
     7441 ccactgattg gtcgtgagaa ggagctggag cgtgctattc aggttctctg ccgtcgccgt
     7501 aaaaacaacc cgctgctggt gggggaatct ggtgtcggta aaaccgcgat tgcggaaggt
     7561 cttgcctggc gaattgttca gggcgatgtg ccggaagtga tggctgactg tacgatttac
     7621 tctctcgata tcggttctct gttagcgggc actaaatatc gcggcgactt tgaaaaacgt
     7681 tttaaagcgt tgctcaagca gctggagcag gacactaaca gcatcctgtt tattgatgag
     7741 atccacacca ttatcggtgc gggggcagcg tctggtggcc aggtcgatgc ggctaacctg
     7801 atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg gttcgacaac ctatcaggag
     7861 ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa aattgatatt
     7921 actgaaccgt cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc gaagtatgaa
     7981 gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cagcggtaga gctggcggtg
     8041 aaatacatta acgatcgtca tctgccggat aaagccattg acgttatcga cgaagcgggc
     8101 gctcgcgcac gcctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt ggcggatatt
     8161 gagtccgtgg tggcccgtat tgcgcgcatt ccagagaaga gtgtttctca gagtgaccgc
     8221 gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca ggataaagcc
     8281 attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca cgaacataaa
     8341 ccggttggtt cgttcctgtt tgccggccct accggggtcg ggaaaacaga ggtgacggta
     8401 cagctttcga aagcgttggg cattgagctg ctgcgctttg atatgtccga gtatatggaa
     8461 cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt tgatcaggga
     8521 ggtttgctga ctgatgcggt catcaagcat ccacatgcgg tgctgttgct ggacgaaatc
     8581 gagaaagcgc atccggacgt gttcaatatt ctgttgcagg tgatggacaa cggtacgctg
     8641 accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat gaccaccaac
     8701 gccggggtac gtgaaactga gcgtaaatcc attggtctta tccaccagga taacagcacc
     8761 gatgcgatgg aggagatcaa gaagatcttt acaccggaat tccgtaaccg tctcgacaac
     8821 attatctggt ttgatcatct gtcaaccgac gtgatccatc aggtggtgga taaattcatc
     8881 gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc tggaagtgag ccaggaagcg
     8941 cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc gatggcgcgt
     9001 gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg ttcgctggtg
     9061 gacggcggtc aggtcaccgt cgcgctggat aaagagaaaa atgagctgac ttacggattc
     9121 cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtta ggtaggttgg
     9181 tcaagtccgt aatctcgaaa gaggttacgg actttttgtt tatggggtgg aggaggttca
     9241 gacccttttt ttaatgatga tggtaagttg ttgataatta gtgctgcggg aaggtaagga
     9301 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9361 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9421 tttgcgaacg cgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9481 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9541 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9601 gtcgcctgac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
     9661 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaagttg tgcagtatat
     9721 ctacatcgag acaagttacg gacttatact tccaaagtac ttcatacata tcacaaaata
     9781 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
     9841 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
     9901 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
     9961 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10021 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10081 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10141 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10201 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10261 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10321 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca tcgtggtacc
    10381 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10441 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10501 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10561 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10621 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg gccgagttcg
    10681 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    10741 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    10801 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    10861 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    10921 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    10981 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11041 accaggcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11101 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ctctgcgtga gtaccttgct
    11161 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11221 gatgggtgac cattaacacc gttttctcac gcatcatttc tgcaagcaat tcaaggatct
    11281 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11341 catgtaacag cgcacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11401 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc aggccaacgc
    11461 gacgcaagct ctccgccaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11521 cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11581 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    11641 ggtcccaagc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    11701 gaatcgcaat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    11761 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    11821 gagtttgggt atcagggaag gtgacttccg gtttttgatc cgttaaatca gtgatccgaa
    11881 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    11941 caaacgcggc taacgcgcag aagacaaaca gggcaattaa cgcgccgggt tgagcattgc
    12001 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagta
    12061 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12121 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12181 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12241 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12301 gtaacataat gccgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12361 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc agataaagat
    12421 gatcgagcgt atcaacatcc gccaccacgc gattgagcaa ttcgccctga cgatagcgcg
    12481 ccagtccggc aggggagagg ggcagcaatt tgctgaaggt gtaaatgcgc agatgctgca
    12541 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12601 tgattgctgc gccacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    12661 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    12721 tggcgagcag cgtgacaatt gccagcacga taccaagact taacatccat ttatgacgtt
    12781 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    12841 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    12901 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    12961 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13021 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13081 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13141 cgccaacagg cgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13201 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13261 cccgcaatgt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13321 atgattctgg tgataaatcg cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13381 aaagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cgaccaacca
    13441 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13501 ccggcgacgt gataaacaga tcctcggcct caatggtcac cggatcggtc gatgctaatt
    13561 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13621 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgc aatggctgga
    13681 aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcaca ccggtatcgt
    13741 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    13801 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc cgtagcactt
    13861 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    13921 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    13981 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatc ccaaccagcg
    14041 ccataaacaa cggaattaac ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14101 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14161 ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccaac gtcgcccagc
    14221 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14281 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14341 agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14401 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14461 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14521 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacgg gatttattca
    14581 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    14641 aaataaaggc gacccatagt cgcatggtgt caccttcttt acttttgtta ctgatttgta
    14701 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    14761 gcctgtaccg gccgaagtaa tggcctgacg ataaatatga tccatcacgt cacccgcggc
    14821 aaagacgcca gggatgctgg tctgggtggc attaccatga atacccgact gcactttgat
    14881 gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc tgtgaccgat
    14941 agcaacaaac agaccagcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15001 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15061 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15121 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15181 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15241 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15301 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15361 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15421 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgct ccattaataa
    15481 cggaccggtc agatcgtttg gatcgccagg ccagttttcc acttccgtgg tggtggtcag
    15541 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15601 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    15661 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    15721 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    15781 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    15841 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    15901 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaac
    15961 agtattctta tatgcgcata accatgcatg taaataccat gtttaccgtg ctagtgaaat
    16021 ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa ggcaacggtc
    16081 ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg tctatcgtga
    16141 cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga aggaatacag
    16201 agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg accgtatcga
    16261 tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg agctttctaa
    16321 acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg aaagacaagg
    16381 gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat cacttctggt
    16441 attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat tcaataccgc
    16501 tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt tcgactacct
    16561 gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg aaaccctgct
    16621 gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag tcaagcagag
    16681 taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg cgtattttga
    16741 ttacactcct gttaatccat acagcaacag tactggggta acctggtact gttgtccgtt
    16801 ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca ggaatacact
    16861 gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct ggaggcgttg
    16921 ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag ctttaaccct
    16981 tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt aggtgggatg
    17041 cccggtgcgt ggttggcaga tacgctgttc tttatttttg gcgtgatggc ttacaccatt
    17101 cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag cgacgaatac
    17161 attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat ccttacctcc
    17221 tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg tggcgtcatt
    17281 ggcagcttac taagtactac gctacaacca ctgctacaca gtagcggggg aactattgcg
    17341 ctgctctgcg tttgggctgc gggcctgacg ctgttcaccg gttggtcatg ggtgaccatt
    17401 gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa tcgtacccgt
    17461 cgcgatgata cctgggtcga tgaagatgaa tatgaagatg acgaagagta tgaagatgaa
    17521 aaccacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc gctagcgcgt
    17581 cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga cgctgcgttg
    17641 ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg cggtgtggct
    17701 gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga atatgacgaa
    17761 tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc agctgctgct
    17821 accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac gccgcctgtt
    17881 gcctctgttg atgttccacc ctcgcaacct acagtagcct ggcagcctgt accgggtcca
    17941 caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca gtcacaatat
    18001 gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc gcagcagccg
    18061 tattatgcac ctgcagctga acaacctgcg caacaacctt actacgctcc tgcggcggaa
    18121 caacctgttc agcaaccgta ttacgcaact gcgccggaac agcctgcaca acagccgtat
    18181 tacgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga agagcagcaa
    18241 tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca accagccgct
    18301 caggagccgt tgtaccaaca gccgcaatcc gttgaacagc agcctgttgt ggagcctgaa
    18361 cctgttgtag aagagacaaa acccgcgcgt ccgccgcttt actattttga agaagtggaa
    18421 gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat tccagaaccg
    18481 gttaaagaac cagaaccgat caaatcttcg ttgaaagcgc cttctgttgc agcagtacct
    18541 ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa agcgacactg
    18601 gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa tagcggtgga
    18661 ccgcgtccgc aagtcaaaga ggggattggc ccgcagttgc cacgaccgaa acgtatccgc
    18721 gtgccaactc gtcgtgaact ggcgtcttac ggtattaagc tgccctcaca gcgtgcggcg
    18781 gaagagaaag cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca gtataacgat
    18841 gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca gacacagcag
    18901 caacgctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga tgcagatgct
    18961 gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg ttattccggc
    19021 gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc gccaatgaaa
    19081 gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc caattgttga acctgtacag
    19141 cagccgcaac aaccggttgc accgcagcag cagtatcagc agccgcaaca accagttccg
    19201 ccacagcagc aatatcagca gccgcaacag ccggttgcgc cgcagccgca gtatcagcag
    19261 ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca
    19321 cagccgcaat atcagcagcc gcaacaacca gttgcgccgc agccgcagta tcaacagccg
    19381 caacaaccgg ttgcaccgca gcagcaggat accctgcttc atccgctgtt gatgcgtaat
    19441 ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga tttgctgaca
    19501 ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat ggctcgcctg
    19561 gtggaagcgc gtctggctga tttccgtatt aaagccgatg tcgtcaatta ctctccgggg
    19621 ccggttatca ctcgctttga acttaacctg gctcctggcg taaaagcggc gcgcatttct
    19681 aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt cgttgaagtt
    19741 attcctggca aaccctatgt aggtctggag ttaccaaata aaaaacgaca aaccgtttat
    19801 ctgcgcgaag ttttggataa cgccaaattc cgcgataatc cgtcgccatt aacagtggtg
    19861 ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat gccgcacttg
    19921 ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat gatcctgagc
    19981 atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc gaaaatgctg
    20041 gagctttcgg tttatgaagg cattccgcat ctgttaacgg aagtcgttac tgatatgaaa
    20101 gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta taaactgatg
    20161 tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga agccgatcgc
    20221 atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga tgcccagcat
    20281 ccggtgctga aaaaagaacc atacattgtg gtgttggttg acgaatttgc cgacctgatg
    20341 atgacggtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa agcccgtgcc
    20401 gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat tactggtctg
    20461 attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat tgactcacgt
    20521 accattcttg atcaggctgg cgcggaatca ctgctgggga tgggggatat gctctactct
    20581 gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga tcaggaagtt
    20641 catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga tggcatcact
    20701 tccgacagtg aaagcgaagg tggcgcgggc ggtttcgatg gcgctgaaga actggatccg
    20761 ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat ttctggcgta
    20821 cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca gatggaagcg
    20881 caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc cccaccgccg
    20941 tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg cactctatca
    21001 actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga atagagagta
    21061 gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa aaattgccat
    21121 cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga
    21181 tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac aaaaagtgac
    21241 tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa
    21301 cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg acggtaaaac
    21361 actgtggttc tataacccgt tcgttgagca agctacggca acctggctga aagatgccac
    21421 cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc agcagtacaa
    21481 tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg gcaatctgaa
    21541 gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg cggtggagca
    21601 ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg tggatgcagc
    21661 gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta agtagaggca
    21721 cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct ctggccgcgc
    21781 gtatgcggcc agaaaattta gcacagtata tcggccagca acatttgctg gctgcgggga
    21841 agccgttgcc gcgcgctatc gaagccgggc atttacattc tatgatcctc tgggggccac
    21901 cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg
    21961 aacgtatttc tgccgtcacc tctggcgtga aagaaattcg cgaggcgatc gagcgcgccc
    22021 ggcaaaatcg caatgcaggt cgccgcacta ttctttttgt tgatgaagtt caccgtttca
    22081 acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc acttttattg
    22141 gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg
    22201 tctatctatt gaaatccctg agtacagagg atattgagca agtactaact caggcgatgg
    22261 aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa acacgacgcg
    22321 ccattgctga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg
    22381 cggatatggc cgaagtcgat gatagcggta agcgggtcct gaagcctgaa ttactgaccg
    22441 aaatcgcagg tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt tacgatctga
    22501 tttccgcact gcataagtcg gtacgtggta gcgcacccga tgcggcgctc tactggtatg
    22561 cgcgaattat taccgccggt ggcgatccgt tatatgtcgc gcgtcgttgc ctggcaattg
    22621 cttccgaaga tgtcggcaat gccgacccac gggcgatgca ggtggcaatt gcggcctggg
    22681 attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag gcgattgttt
    22741 acctagcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagcc gcgctggcgg
    22801 atgctcgcga acgtccggat tatgacgtgc cggttcattt gcgtaatgcg ccgacgaaat
    22861 taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt
    22921 atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat tatttcccga
    22981 caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct gaacaggatc
    23041 aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt gttactgtat
    23101 ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac aggataagca
    23161 tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc
    23221 ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt aaagtattgc
    23281 aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc attggccagg
    23341 cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag
    23401 agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc gatatcgcgc
    23461 tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa aatgacaacg
    23521 tcgaagtcag ccgctggggc accccgcgtg agtttgactt tgaagttcgt gaccatgtga
    23581 cgctgggtga aatgcactct ggcctcgact ttgcagccgc agttaagctg accggttccc
    23641 gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg cagtttatgc
    23701 tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac ctggttaacc
    23761 aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg ttccatactc
    23821 gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaacg gcagaagttc
    23881 cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca attaagatga
    23941 ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac acccgtggtc
    24001 tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc ccggaagact
    24061 caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag ttgctgggcc
    24121 tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct tgcaaaactt
    24181 acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct tcctgctcca
    24241 acgtttggga tttccaggca cgtcgtatgc aggcacgttg tcgcagcaag tcggacaaga
    24301 aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt acgctggttg
    24361 cggttatgga aaactatcag caggctgatg gtcgtattga agtaccagaa gttctgcgtc
    24421 cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat ctaaaaagcg
    24481 cctgcgggcg ctttttttgt ctccctttga taccgaacaa taattactcc tcacttacac
    24541 gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt attcgatgta
    24601 tacaagccta tatagcgaac tgctatagaa ataattacac aatacggttt gttactggaa
    24661 tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga tgcggtattg
    24721 gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg cctggcaatg
    24781 gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga tagcgccatt
    24841 ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta cagttaactg tggtagtcgc
    24901 tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac ggacaatacc
    24961 ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gtctgcgtgg gcgttccatg
    25021 cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga tgaaacgagt cggggcgcgc
    25081 ggtgaaggca aattcgagcg cattagctgg gaagaagcct acgacatcat cgcgaccaat
    25141 atgcagcgcc tgatcaaaga gtacggcaac gagtctatct atctgaacta tggcaccggt
    25201 acgctgggcg gcaccatgac ccgctcctgg ccgccgggaa ataccctggt cgcgcgcctg
    25261 atgaactgct gcggcggtta tcttaaccat tacggtgact actcttctgc gcaaattgcg
    25321 gaaggtttga actataccta cggcggctgg gcagatggta acagcccgtc ggatatcgaa
    25381 aacagtaaat tggtagtgct gtttggtaat aacccaggcg aaacgcgaat gagtggcggt
    25441 ggggtgactt actatcttga acaggcacgc cagaaatcta atgcccgcat gatcatcatc
    25501 gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc tattcgtcca
    25561 ggaacagatg ccgcactggt taacggtctg gcgtatgtca tgatcaccga aaacctggtg
    25621 gatcaggcat tccttgataa atattgcgtt ggctacgatg agaaaaccct gcccgctagc
    25681 gcgccgaaaa atggccacta taaagcttat attctgggtg aagggccaga tggcgtggct
    25741 aaaacaccgc aatgggcctc acaaatcacc ggtgttccgg cagacaaaat catcaaactg
    25801 gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc agggatgggg cccgcagcgt
    25861 cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct gaccggtaac
    25921 gttggtatta acggaggcaa cagcggcgcg cgtgaaggtt catacagctt accgtttgtc
    25981 cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat gtggaccgat
    26041 gccattgaac gtggcccgga aatgacagcg ctgcgtgatg gtgtacgcgg gaaagataag
    26101 ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctgat taaccagcat
    26161 tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga gctgattgtg
    26221 gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct gcctgactgc
    26281 accgcttccg aacagatgga ctttgcactg gatgcatcct gcgggaatat gtcttacgtg
    26341 attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta tgaaatgacc
    26401 agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg tacacaggaa
    26461 gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact gccaacgttt
    26521 gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca ccacgttgct
    26581 tataaagctt tccgtgaaga tcctcaggca aatccactga ccacgccatc gggcaaaatt
    26641 gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc agaaggcgat
    26701 gtaatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga tccgttgaac
    26761 aaacagtatc cgctgcagct tacgggtttc cactataaat ctcgcgttca ctcaacttac
    26821 ggcaacgttg atgtgctgaa agcagcttgc cgtcaggaaa tgtggatcaa cccgcttgat
    26881 gcgcaaaaac gcggtatcaa caacggcgat aaagtcagga tctttaacga tcgtggtgag
    26941 gttcatattg aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc actgggtgaa
    27001 ggtgcctggt atgacccgga tacaaaacgt gtcgataagg gcggttgtat caacgtactg
    27061 accactcaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa ccttgttcag
    27121 gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta ttgattccag
    27181 ccgttgcacc ggttgcaaaa cctgcgagct ggcctgtaaa gactacaaag atttgacgcc
    27241 agaagtcagc ttccgccgca tttatgaata tgctggcggc gactggcagg aagataacgg
    27301 tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcatgtaacc actgcgaaga
    27361 tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag atggttttgt
    27421 tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt gcccgtatgg
    27481 cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt gttatgaccg
    27541 tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg cgctggattt
    27601 cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg cgccgttgcc
    27661 gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata gccgcccgac
    27721 cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga agtggatggc
    27781 atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtagcaggt ggttttatcg
    27841 ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag cgtgttatcg
    27901 cctgcatgtt tggtttatgg gtgctgatgg gcattggctt tatcgcctct atgctccatc
    27961 ttggttcacc aatgcgcgct tttaactcgc tcaaccgggt aggggcttca gcactcagta
    28021 acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg catcggctgg ttgctggcaa
    28081 tgctgaaaaa gctgtcaccg gcattgcgta cgctgtggct gatagtgacg atggttcttg
    28141 gcgtcatctt tgtctggatg atggtgcgtg tgtataacag cattgatacc gttccgacct
    28201 ggtacagcat ctggacgccg atgggcttct tcctgacgat gtttatgggc ggcccgttac
    28261 tgggttacct gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt ctgctgccag
    28321 caatttctgt actggcactg gtagtgagtg gcgtggtgtc agtgatgcag ggcgcagagc
    28381 tggcgactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc
    28441 tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg cttgtggatt gcaccacagc
    28501 taaaaggtta tcagcctgcg gttccgctac tatccgtctc attcattctg cttctggcag
    28561 gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg atggccgtcg
    28621 caagctaatc agtaacaacc ggggtttcgg ccccggttct cttttatttc tgcttcgtta
    28681 acgtgtcata actggtcatc aaattacgat aatcaggaat gtggttagag aacaacgtcg
    28741 ccagtccttc aatatcatta cgccagtcgc gatgcagctc gcacgccacg ccgaaccatg
    28801 tcatcaattg cgcaccagct tgcgacatgc gatcccatgc cgaatgccgg gtaatttcat
    28861 taaaggtccc tgacgcgtca gtcacgacaa agacgtcaaa cccttcttca atggctgaaa
    28921 gcgccgggaa tgccacgcaa acttcggtta ccacaccggc aataattaac tgttttttac
    28981 ctgtcgcttt gacagctttt acaaaatctt cgttatccca ggcgttaata tttcccgggc
    29041 gagcaatgta aggtgtatcg gggaattgtg ctttcagttc tggaactaat gggccgttag
    29101 gaccggtttc aaaactggtg gtaagaatag ttggtaagtt gaaatactta gccaggtcac
    29161 ccagcgccag cacgttattt ttaaacttat caggttcgat atcccgtaca agggaaagta
    29221 aaccagcctg gtgatcaaca agcaaaacgg cagcatcatt tttatcaaga cgaacatacg
    29281 gtttggtcat cctcttctcc tttcgagagt cagtggtacg tctgaaatcg atcagatgcg
    29341 atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat gaaatttttc
    29401 gactgaacga agcgtatgaa a
//
LOCUS       DAFAVF010000047        29320 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFAVF010000047 DAFAVF010000000
VERSION     DAFAVF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.047"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            451..1728
                     /gene="waaA"
                     /locus_tag="LGK42_001998"
     CDS             451..1728
                     /gene="waaA"
                     /locus_tag="LGK42_001998"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBK2658250.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1736..2215
                     /gene="coaD"
                     /locus_tag="LGK42_001999"
     CDS             1736..2215
                     /gene="coaD"
                     /locus_tag="LGK42_001999"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK2658251.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(2254..3063)
                     /gene="mutM"
                     /locus_tag="LGK42_002000"
     CDS             complement(2254..3063)
                     /gene="mutM"
                     /locus_tag="LGK42_002000"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBK2658252.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(3161..3328)
                     /gene="rpmG"
                     /locus_tag="LGK42_002001"
     CDS             complement(3161..3328)
                     /gene="rpmG"
                     /locus_tag="LGK42_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK2658253.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3349..3585)
                     /gene="rpmB"
                     /locus_tag="LGK42_002002"
     CDS             complement(3349..3585)
                     /gene="rpmB"
                     /locus_tag="LGK42_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK2658254.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3802..4470)
                     /gene="radC"
                     /locus_tag="LGK42_002003"
     CDS             complement(3802..4470)
                     /gene="radC"
                     /locus_tag="LGK42_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK2658255.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4642..5862
                     /gene="coaBC"
                     /locus_tag="LGK42_002004"
     CDS             4642..5862
                     /gene="coaBC"
                     /locus_tag="LGK42_002004"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBK2658256.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5840..6298
                     /gene="dut"
                     /locus_tag="LGK42_002005"
     CDS             5840..6298
                     /gene="dut"
                     /locus_tag="LGK42_002005"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK2658257.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6405..7001
                     /gene="slmA"
                     /locus_tag="LGK42_002006"
     CDS             6405..7001
                     /gene="slmA"
                     /locus_tag="LGK42_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBK2658258.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7038..7679)
                     /gene="pyrE"
                     /locus_tag="LGK42_002007"
     CDS             complement(7038..7679)
                     /gene="pyrE"
                     /locus_tag="LGK42_002007"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK2658259.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7745..8461)
                     /gene="rph"
                     /locus_tag="LGK42_002008"
     CDS             complement(7745..8461)
                     /gene="rph"
                     /locus_tag="LGK42_002008"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK2658260.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8588..9451
                     /locus_tag="LGK42_002009"
     CDS             8588..9451
                     /locus_tag="LGK42_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK2658261.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9672..10496
                     /gene="dinD"
                     /locus_tag="LGK42_002010"
     CDS             9672..10496
                     /gene="dinD"
                     /locus_tag="LGK42_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBK2658262.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10788..11405
                     /locus_tag="LGK42_002011"
     CDS             10788..11405
                     /locus_tag="LGK42_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBK2658263.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11402..13084)
                     /gene="ligB"
                     /locus_tag="LGK42_002012"
     CDS             complement(11402..13084)
                     /gene="ligB"
                     /locus_tag="LGK42_002012"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBK2658264.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            13342..13965
                     /gene="gmk"
                     /locus_tag="LGK42_002013"
     CDS             13342..13965
                     /gene="gmk"
                     /locus_tag="LGK42_002013"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK2658265.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            14020..14295
                     /gene="rpoZ"
                     /locus_tag="LGK42_002014"
     CDS             14020..14295
                     /gene="rpoZ"
                     /locus_tag="LGK42_002014"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK2658266.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            14314..16422
                     /gene="spoT"
                     /locus_tag="LGK42_002015"
     CDS             14314..16422
                     /gene="spoT"
                     /locus_tag="LGK42_002015"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK2658267.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16429..17118
                     /gene="trmH"
                     /locus_tag="LGK42_002016"
     CDS             16429..17118
                     /gene="trmH"
                     /locus_tag="LGK42_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBK2658268.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            17124..19205
                     /gene="recG"
                     /locus_tag="LGK42_002017"
     CDS             17124..19205
                     /gene="recG"
                     /locus_tag="LGK42_002017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK2658269.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(19190..20056)
                     /locus_tag="LGK42_002018"
     CDS             complement(19190..20056)
                     /locus_tag="LGK42_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658270.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(20059..21264)
                     /gene="gltS"
                     /locus_tag="LGK42_002019"
     CDS             complement(20059..21264)
                     /gene="gltS"
                     /locus_tag="LGK42_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBK2658271.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            21544..22935
                     /gene="xanP"
                     /locus_tag="LGK42_002020"
     CDS             21544..22935
                     /gene="xanP"
                     /locus_tag="LGK42_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBK2658272.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            23056..24765
                     /locus_tag="LGK42_002021"
     CDS             23056..24765
                     /locus_tag="LGK42_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBK2658273.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(24818..27136)
                     /gene="yicI"
                     /locus_tag="LGK42_002022"
     CDS             complement(24818..27136)
                     /gene="yicI"
                     /locus_tag="LGK42_002022"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBK2658274.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLIAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(27146..28528)
                     /locus_tag="LGK42_002023"
     CDS             complement(27146..28528)
                     /locus_tag="LGK42_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBK2658275.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            28821..28915
                     /locus_tag="LGK42_002024"
     tRNA            28821..28915
                     /locus_tag="LGK42_002024"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28855..28857,aa:Sec,seq:tca)
BASE COUNT         7187 a         7922 c         7348 g         6863 t
ORIGIN      
        1 atcttaatga gcaaaattct tcgaaatggc ttatccacaa gtaactcttt ttgttgagat
       61 tatccgatta aggtgaagca gaaaaagcac gctaccgccc caggctcaac agctacctga
      121 atactgatac cgcactgata ttgacgcgct agtgtatcac ttctttcgcg gcaatccgac
      181 ccgaatgatt tttaagcaca ttgttgattt tcaataaaga aatcaatcaa ataaaatcat
      241 tagcaaccat attctttcac tgttgctctg cgattccttc ctgcacgtac agcagaaatc
      301 aatgaatcgc ataaaaatac aaaaaaagta tagatgttca gcaagaattg gcgggtaaat
      361 tgctatttaa gtcaaaaacg ggaaaagtaa tggtaaagcc acagctaaat acatagaatc
      421 cccagcacat ccataagtca gctatttact atgctcgaat tgctttacac cgcccttctc
      481 taccttattc agccgctgat ctggatacgg ctctgggtgc gcggacgtaa ggctccggcc
      541 tatcgaaaac gctggggtga acgttacggt ttttaccgcc atccgctaaa accaggcggc
      601 attatgctgc actccgtctc cgtcggtgaa actctggcgg cgatcccgtt ggtgcgcgcg
      661 ctgcgccatc gttatcctga tttaccgatt accgttacaa ccatgacgcc aaccggttcg
      721 gagcgcgtac aatcggcttt cgggaaggat gttcagcacg tttatctgcc gtatgacctg
      781 cccgatgcgc tcaaccgttt cctgaataaa gtcgacccca aactggtgtt gattatggaa
      841 accgaactat ggcctaacct gattgcggcg ctacacaaac gtaaaattcc gctggtgatc
      901 gctaacgcgc gactctctgc ccgctcggcc gcaggttatg ccaaactggg taaattcgtc
      961 cgtcgcttgc tgcgtcgtat tacgctgatt gctgcgcaaa atgaagaaga tggtgcacgt
     1021 tttgtggcgc tgggcgcaaa aaataatcag gtgaccgtta ccggtagcct gaaattcgat
     1081 atttctgtaa cgccgcagtt ggctgctaaa gccgtgacgc tgcgccgcca gtgggcacca
     1141 caccgcccgg tatggattgc caccagcact cacgaaggcg aagagagtgt ggtgatcgcc
     1201 gcacatcagg cattgttaca gcaattcccg aatttattgc tcatcctggt accccgtcat
     1261 ccagaacgct tcccggatgc gattaatctt gtccgccagg ctggactaag ctatatcaca
     1321 cgctcttcag gggaagtccc ctccaccagc acgcaggttg tggttggcga tacgatgggc
     1381 gagttgatgt tactgtacgg cattgccgat ctcgcctttg ttggcggttc actggttgaa
     1441 cgtggtgggc ataatccgct ggaagctgcc gcacacgcta ttccggtatt gatggggccg
     1501 catactttta actttaaaga catttgcgcg cggctggagc aggcaagcgg gctgattacc
     1561 gttaccgatg ccactacgct tgcaaaagag gtgtcctctt tactcaccga cgccgattac
     1621 cgtagtttct atggccgtca tgccgttgaa gtactgtatc aaaaccaggg cgcgctacag
     1681 cgtctgcttc aactgctgga accttacctg ccaccgaaaa cgcattgagg ttgttatgca
     1741 aaaacgggcg atttatcctg gtactttcga tcccattacc aatggtcata tcgatatcgt
     1801 gacgcgcgcc acgcaaatgt tcgatcacgt tattctggcg attgccgcca gccccagtaa
     1861 aaaaccgatg tttaccctgg aagagcgtgt ggcgctggca cagcaggcaa ccgcgcatct
     1921 ggggaacgtg gaagtggtcg ggtttagtga tttgatggcg aacttcgccc gtaatcaaca
     1981 cgctacggtg ctgattcgtg gcctgcgtgc ggtggcggat tttgaatatg aaatgcagct
     2041 ggcgcatatg aatcgccact taatgccgga actggaaagt gtgtttctga tgccgtcgaa
     2101 agagtggtcg tttatctctt catcgttggt gaaagaggtg gcgcgccatc agggcgatgt
     2161 cacccatttc ctgccggaga atgtccatca ggcgctgatg gcgaagttag cgtagcgttt
     2221 atgccggatg gtatgccatc cggcgcgcat gaattacttc tggcactgcc gacaataaaa
     2281 cgttgcccgc tgcgcatgtt tagtcgccac aatcggcgta ccgcacaccc gacacggctc
     2341 gccttttcgc ccgtaaacct gcaattcctg cgcgaaatag cccggtttac catcactttg
     2401 cagaaaatct ttcagcgttg taccaccctg ctcaatcgaa cgcagcaaca ccgctttaat
     2461 cacccgagct aacaattcac actctgccag cgacagtgat gacgccagcc gatccggatg
     2521 gatccccgcc gcaaacagtg attcgctggc atagatattc cctaccccta ccaccagctt
     2581 gttatccatc agccacggtt taatcgccgt ttttttcttc gcgcacttct gatgcagata
     2641 ctcaccattg aaatcgtcgc tgagcggctc cggtccaaga tgggccagca cattatgccc
     2701 ttccagctct ttggtccaca gccaggcacc aaagcggcgc ggatcggtgt agcgcagcac
     2761 tttaccgttg ctcatcacca aatccacatg gtcatgcttt tcagggggaa gttcttctgg
     2821 aaggatgcgc aggctgccag acatccccaa atgaatgata atccagccct caggcagctc
     2881 cagcagcaaa tatttagccc gccgctgcac gctaagcact ggttggtcgc ttaaacggta
     2941 gatctcttct gaaaccggcc agcgcaagcg tccgttgcgc accactgcat gaagaatggt
     3001 tgcaccaacg agatgcggtt ctatgccgcg acggctggtt tcaacttcgg gtaattcagg
     3061 catagcatct ccaggaatga acagatgcaa tcaatatggg ggcaagcaga taacaaaaaa
     3121 ccccgccgga gcgaggtttt ttgttacatc aaagcgagaa ttatttgatt ttcgcttctt
     3181 tgtagatcac gtgctgacga acaactggat cgaatttttt cagttccagt ttttccggct
     3241 tagtacgttt gttcttcgta gtggtataga agtgaccagt accagcagaa gaaaccagct
     3301 tgattttctc acgaatacct ttagccatga tttatttcct ctaagtactt agtacttttc
     3361 gccacgggca cgcagttcag ccagaactgt atcgatgcct tttttatcga ttacacgcat
     3421 acctttagca gatacgcgca gggtgacaaa acgcttctcg ctctcaaccc agaaacggtg
     3481 agagtgcagg ttcggcagga aacggcgttt agtcgcgttc agtgcgtggg aacggttgtt
     3541 accggtcacc ggacgcttgc cagtaacttg gcagactcgg gacatgtcta ttctccaaaa
     3601 atcaaattag ctcgagcttc gtatggggta ttggcgcctc gtcaggcttt acagcccggt
     3661 catcgcagtt ctatgtgaac tctcgattgc caggcccaaa tgccaaaccc gagattctca
     3721 aaggtggcgt agtatacgct gactcagcga tgtgctcaag tcccgaacag acaaagatcc
     3781 cgaaggatcg cgcatagcgg gttaaatcca gccgcgttcg gcaaaagaaa catactctcc
     3841 acgcccaatc acgatatggt cgagcacgcg taaatccatg aactgacaac tctttattat
     3901 ccgttcagta atgagtttat ccgctttact gggttcagca caacccgaag ggtgattatg
     3961 tgcaaggatc agcgccgagg cgtttatttt tatcgcttcg cggataattt cccgaggatg
     4021 gacttcaaca tggtttagcg tgccggaaaa aagtcggcta tgcgttataa cccggtgttg
     4081 tgagtcgaga aagatcacca taaagatctc ccgctcctca cccgtgagct ggctttgtaa
     4141 aaattcccgc gtcatctccg ggctgagtaa agggctttct tcacgcatcc gcacgttgta
     4201 gtaacgccgc gccagttcag caatcccctt taactgggca aatttcgcca cgccaattcc
     4261 atgaacgcca ctaaattgtt catattcaga ggttaacaag ccataaagag agccgaaatt
     4321 ctccagcatc tcttttgcca gggttaatac atctttaccg cgcgttccgg tacgcagaaa
     4381 tagcgccagc agctcgacat ccgttaaggc gctaatacca aacttcagca ttttttcgcg
     4441 cggcatcaac agctgtgaat tgtttttcac cttcacctcc tttgtggtgc tcgcatcctg
     4501 tcatagccgt tccgttaaat cgacggccag ttttcaatcc tggaaagcgc ctcgcaaagt
     4561 gattctcagg tgaaggcacg acaacaaaag tattgtgata aaatcgccaa cttctggtgt
     4621 cacccaacag gaaaaatcat catgagcctg gccggtaaaa aaatcgttct cggcgttagc
     4681 ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc gtttgcgcga tcgcggggcc
     4741 gacgtccgcg tagccatgac cgaagcggca aaagccttta tcaccccact tagcttgcag
     4801 gcggtttctg gttatcccgt ttccgacagt ctgctggacc cggcagccga agccgctatg
     4861 ggccatattg agctgggtaa atgggctgat ttagtgattc tcgcccctgc cacggcagat
     4921 ttgattgccc gtgttgctgc cggaatggcg aatgacctgg tatcgacgat ttgtctggct
     4981 acacctgcgc ctgtagccgt gctccccgcc atgaaccagc agatgtaccg tgccgctgcc
     5041 acgcagcata atttagaggt gcttgcttcc cgtggtttgc tcatctgggg gccagacagt
     5101 ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc tcgatccgtt aaccattgtg
     5161 gatatggcgg tagcgcattt ttcgcccgtc aacgacctga aacatctgaa cattatgatt
     5221 accgccggcc cgacgcgtga accgctcgat cccgtgcgtt atatctctaa tcacagctcc
     5281 ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc gtggcgcgaa cgtcacgctg
     5341 gtatcaggtc cggtttcact accgacgcca ccgtttgtta aacgtgttga tgtgatgacc
     5401 gcgctggaaa tggaagccgc cgtgaatgct tctgtacagc agcaaaatat ttttatcggc
     5461 tgcgccgccg tggcggatta tcgcgcagct accgtggccc cagagaaaat caaaaagcag
     5521 gccacgcagg gtgatgaatt aacaataaaa atggttaaaa accccgatat cgtcgcaggc
     5581 gttgccgcac taaaagacca tcgaccctac gtcgttggat ttgccgccga aacaaataat
     5641 gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc ttgatctgat ctgcgcgaac
     5701 gatgtttccc agccaactca aggatttaac agcgacaaca acgcattaca ccttttctgg
     5761 caggacggag ataaagtctt accgcttgag cgcaaagagc tccttggcca attattactc
     5821 gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt aagattctgg acccgcgcgt
     5881 tgggaaggaa tttccgctcc cgacttatgc cacctctggc tctgccggac ttgacctgcg
     5941 tgcctgtctc gacgacgccg tagaactggc tccgggtgac actacgctgg ttccgaccgg
     6001 gctggcgatt catattgccg atccttcact ggcggcaatg atgctgccgc gctccggatt
     6061 gggacataag cacggtatcg tgcttggtaa cctggtagga ttgatcgatt ctgactatca
     6121 gggccagttg atgatttccg tgtggaaccg tggtcaggac agcttcacca ttcaacctgg
     6181 cgaacgcatc gcccagatga tttttgttcc ggtagtacag gctgaattta atctggtgga
     6241 agatttcgac gccaccgacc gcggtgaagg cggctttggt cactctggtc gtcagtaaca
     6301 catacgcatc cgaataacgt cataacatag ccgcaaacat ttcgtttgcg gtcatagcgt
     6361 gggtgccgcc tggcaagtgc ttattttcag gggtattttg taacatggca gaaaaacaaa
     6421 ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct ggcgctgatg ctggaatcca
     6481 gcgatggaag ccaacgtatc acgacggcaa aactggccgc ctctgtcggc gtttccgaag
     6541 cggcactgta tcgccacttc cccagtaaga cccgcatgtt cgatagcctg attgagttta
     6601 tcgaagatag cctgattact cgcatcaacc tgattctgaa agatgagaaa gacaccacag
     6661 cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg tgagcgtaat cctggcctga
     6721 cccgcatcct cactggtcat gcgctaatgt ttgaacagga tcgcctgcaa gggcgtatca
     6781 accagctgtt cgagcgtatt gaagcgcagc tgcgtcaggt attgcgtgaa aagagaatgc
     6841 gtgagggtga aggttacacc accgatgaaa ccctgctggc aagccagatc ctggccttct
     6901 gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa ataccgcccg acggatgatt
     6961 ttgacgcccg ctggccgcta attgccgcgc agttgcagta atatgacgcc ggatgacttt
     7021 tcatccggcg agtttcttta aacgccaaac tcttcgcgat aggccttaac cgccgccaga
     7081 tgttccgcca tttccggctt ctcttccaga taggcaatca gatctttcag ggtgatgata
     7141 gagatcactt tgcagttgta atcacgctca acttcctgaa tcgccgaaat ctcgccgcgc
     7201 ccccgttcct gacgatcaag cgaaatcaat acgccagcaa gcgtcgcgcc attagcctga
     7261 ataatctcca tcgattcgcg aatcgccgtt ccggcggtga tcacatcatc taccagcatt
     7321 acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt caccgtggtc ttttgcctct
     7381 ttgcggttaa agcagtacgg caggtcaagg tcatgatgct ccgccagcgc cacggcggta
     7441 gtggtcgcaa tcgggatccc tttgtaagca gggccaaaca gcagatcgaa ctcaaggccg
     7501 gaatccacca acgcttcagc gtaaaaacgg cctaacagtg ccagatcgcg cccggtatta
     7561 aacagcccgg cgttgaagaa ataggggctt ttgcgcccgg acttcaacgt aaactcgcca
     7621 aactttaaca cctgcttgcc aagcgcaaat tcaataaact ggcgctgata tggtttcatg
     7681 ccttcgctcc tcatcttact tttctacaga caaaaaaaag gcgactcatc agtcgcctta
     7741 aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat ggattcgatt ccccctcggg
     7801 ccagagccaa caaggtgagt agctcttcat gggtgaacgg ctcgccttct gccgtcccct
     7861 gcacttcaat gatgcgcccg tcttcggtca tcactacgtt catgtcggtc tctgcggcag
     7921 agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt cacaattccg acagaaactg
     7981 cggctaccat ccctttcatc ggattggttt tcagcttgcc gttttccacc agcttctgta
     8041 gcgcatctgc cagcgccacg caggcacccg taatcgacgc ggtacgcgtg ccaccatcag
     8101 cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc cagcgctttc aaatctactg
     8161 ccgcgcgaag agcacgggcg atcagacgct ggatttccat tgtgcgtcca ccctgcttac
     8221 ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg tggcagcatg ccgtactctg
     8281 cggtgatcca gccctggccc tgacctttca ggaagcgcgg cacgccttct tcaatagagg
     8341 cggtacacaa cactttggta tcgccaaatt cgaccagcac cgagccttct gcatgttttg
     8401 tatagttacg agtcagggta acgggacgca cctgattatt gctacggcct gctggacgca
     8461 tattgaaatc tccggcttga aacaaatgtg gctgcgcatt atacggactt ccggcggtta
     8521 ttcctatcct gacaaggcat cgatggctat aatccttcca cctctccttt tataaacagg
     8581 aacgtctatg atccgcagta tgaccgccta cgcccggcgt gaaatcaagg gtgaatgggg
     8641 gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat ctggaaactt actttcgtct
     8701 gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag cgtattcgtt ctcgcctgac
     8761 gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat gttagcgcgc aaggtgagct
     8821 gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc gcgaactggg taaaaatgca
     8881 gagtgacgaa ggggaaatca acccggttga tattctacgc tggccgggcg tgatggcagc
     8941 ccaggagcag gatcttgacg ccattgccgc tgaaattctc gcggcgctgg atggtacgct
     9001 ggacgacttt attgtcgcgc gcgaaaccga aggtcaggca ctgaaagcat tgatcgagca
     9061 gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc gcccatatgc cggaaatcct
     9121 gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat gctcaggttc agttggaaaa
     9181 caaccgtctg gagcaggagc tggttctgct ggcacaacga attgacgttg ccgaagaact
     9241 ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt ctgaagaaaa aagaagccgt
     9301 tggtcgtcgt ctggacttta tgatgcagga attcaaccgc gagtcgaaca ctcttgcgtc
     9361 gaagtctatc aatgccgaag tgacaaactc cgcgattgag ctgaaagtgt tgattgagca
     9421 gatgcgcgag cagattcaga acattgaata actcgtaacg ccaattctta cttttcagcc
     9481 ttcacaaatg ccgcccactc aaacagagcg gcatttttct tccccgcaac ttcaattctg
     9541 ttttgcgtgc ctgctccaga ttttgcgatg tttttttgcc cagcacactg agaacgtgag
     9601 atactcacaa ctgtatataa atacagttac agatttactt tctttgcaat cgatatcaca
     9661 tggagtgggc aatgaacgaa catcatcaac cttttgaaga aatacggcat tatggcactg
     9721 aagggcaaga attttggtca gctagagaat tggcaccgtt actggattat cgggactggc
     9781 gcaattttca aaaggtactg gcccgcgcga ctcaagcgtg cgaagccagc aatcaagctg
     9841 catctgacca tttcgttgaa accaccaaaa tggttgtatt aggttcaggg gctcaacgag
     9901 aacttgaaga tgttcatctc tcccgctatg cctgttacct ggtagtacaa aacggcgacc
     9961 ctgcgaaacc ggttattgcg gcagggcaaa cttattttgc tatccagacc cgacggcagg
    10021 agctggctga cgatgaagct ttcaggcaac ttcgtgaaga cgaaaagcgt ctttttctga
    10081 gaaatgaatt gaaagaacat aacaaacaat tggttgaggc agcgcagcaa gctggggtag
    10141 cgacagctat cgatttcgcc atatttcaga atcatggtta ccgggggctg tatggtggat
    10201 tagatcagaa agccatccat cagcggaagg ggctgaaaaa gagtcagaag atcctggatc
    10261 atatgggttc aacagaactg gcggctaatc tctttcgagc tacccaaaca gaagaaaaac
    10321 tcaagcggga tggcgtgaat tcaaaacagc aagctaatac cacacacttt gacgtgggta
    10381 gcaaggtgag gcaaaccatt caggaacttg gcggaaccat gcctgaggag ttgccgaccc
    10441 cgcaggtcag catcaagcag ttggaaaaca gtgtaaaaat tacagagaag aaatagcaga
    10501 aactaaaaac tctttttgtt gattgagata cccgatgcgt aagccaaagt ccaggtgcgg
    10561 tgaacacaat ggctaaacat tgcacctttc ttttccaccc ctcaatttta acctattttt
    10621 tcttatgcat tttctcagac aagaagtcag aagaatgcat ctacgctaca gaaaatagcg
    10681 atttcacata actacaagtt atcaatttcc tctcacctta aaaaatctca atcgtgacaa
    10741 tacgcacaaa tcgctaccct gccagacaga tttttaggga gagaaccatg ctgttacaca
    10801 ttttgtattt ggttggcatt actgccgaag ccatgacggg ggctctggcg gccgggcgac
    10861 gccgcatgga tacatttggc gtaattatta ttgcgaccgc caccgcaatt ggcggagggt
    10921 cagtgcgcga tattctgctg ggccactatc cgctcggctg ggtcaaacac ccggaatatg
    10981 tgattatcgt cgctaccgcc gcagtactca ccactatcgt tgctcctgta atgccttacc
    11041 tgcgcaaagt gtttctggtg ctcgatgcgc ttggactggt ggtcttttct atcatcggcg
    11101 cacaggttgc actggatatg gggcacggcc caattattgc cgttgtcgcg gcggtaacca
    11161 ccggcgtgtt tggcggcgtt ttgcgcgata tgttctgtaa acgtatccca ctggtattcc
    11221 agaaagagtt gtatgccggt gtctcatttg cctccgccgt gctgtacatc gcgctgcaac
    11281 actatgtttc taaccatgat gtggtaatta tctccaccct ggtattcggc ttttttgccc
    11341 gcttactggc gctacgcctt aagctgggat taccggtctt ttactacagc cacgaaggac
    11401 actaaggttc aaaacctgtg atctgctggg cagccagcca actgcccagc ttcttgattt
    11461 gcgcattttc cttccattca ataacctgtc tggcgcgccc cgatccagtc cccggcagct
    11521 gctgccagaa ctgctccgtg ctaaataaaa gttgcgacca ggaccgttca tcactggcat
    11581 taagcgccgc ccgggttagc ggtattccca ttgccatcac ccagcgagta aaaggctgct
    11641 tacgagccag attaaaccga tgccatagct gcgcactttt acttttcgcg atccccggcg
    11701 tgttctgtaa ttgctctggc gttaataaaa gccaggaaaa gatatgctca aagcgatgag
    11761 tctgatgcag cgcgcgccag ccggcctcac caataccatc cagccccaga acctgttttg
    11821 accccagcca gactaagcgt gaaatgaact gttcctgaca aacatcagaa gcaaagtagc
    11881 aggtcaacga gttaaagcgg ttttctggtg gtgtcggttt tgtacgttct gcaccgcgcc
    11941 agaccacatc atcaatgcga ggaataccct gaccggcaag gctgacgagg atctgatcac
    12001 caggcgcaat atcccactcc tgccagcgcc tgacggaacc aatattcacc cgctggactt
    12061 ttttatcatc cagcatgaca ggtgcgagtg acgcaaccac cgatatttta ccgctcttac
    12121 ccaccgcaaa ctgaatcgcc ttcacttcgg caacctgagc tacaggttga tatttccagg
    12181 ccaccagcca ttctgcctgg cctggtagcc aatggcggga ttcgggttct ttcgcccctc
    12241 gcacaaccac gccatcggtg acgaagggta atttcgcttt ccaccactca ttgcgtacgc
    12301 gcgcaacctc atcagcattt ttaaccgcac gggtatacct ctgcgttaga gtaaaacctg
    12361 cggtagccag ctcctttaaa cgatcggtca ttaactgcgg tccatccggc catgcccaga
    12421 caaaaacgcc cagagaattc agcgtgtcgc tatcgtcctg gcgcatcatc aggccagcaa
    12481 cttttgcgcg ggcatttatt ccccccattt gttgttggat atgcccctcg cgctggagaa
    12541 atatttcccc ctgaagcgta ctgttggcta aaggcccgct aacggtttgc ggcacagcgg
    12601 aaattaagct aactttctgc gtccagtcct cgcctttcag gccattaccg cgactgattg
    12661 ctttgttcag tttcccgtcc cgataaacca gggttaccgc aacgccatca acttttggct
    12721 gcacccaaag atcgctacgt tctcgcatcc acagacttaa tgcgttttta tccaccattt
    12781 tgcgtacacc cgtatgggca accggatgca taaccgcacc atttaaaggc ggcatcatca
    12841 cgtcgcgggg ctcgctccca aagcagcgtt gccactgcgt aagacgggcg cttaactgat
    12901 cgtaaacacc gtcttccacc tcactttttc cttccttcca gtagtcatcg tcccactgtt
    12961 ttatttgctg ttgcaggcgg gaaatttctt cctgtgctct ggctggcgac caggccggac
    13021 agaccgccca cacagatgat tgccagcaca agatacttat taatatcgcc atccatactt
    13081 tcatcatcac ctccgctgta ggtagtcagg cagatataca acgtgatgaa agcaaagccg
    13141 agtggcaaaa acggagtctg cgaggacgct tcctgaaaat cgtctttatt gcagtgaatg
    13201 acaggcaaat gcggaagcag ctacgcaaaa cgcaacaact ttgcgcaaaa agtgtgagca
    13261 agggctacgt cacatggccg cgccgtgtat aataagctcg tatgtaggct ttatttcgct
    13321 aatcacatac gaaagatact catggctcaa ggcacgcttt atattgtttc tgcccccagt
    13381 ggcgcgggta aatccagcct gattcaggct ttattaaaaa cccaaccgtt gtatgacacc
    13441 caggtttctg tttcacacac cacacgccaa ccgcgtcctg gtgaagtcca cggtgaacat
    13501 tatttctttg ttaatcatga tgaatttaaa gaaatgatta gcagagatgc gttcctcgaa
    13561 cacgcagaag tttttggtaa ttactatggc acttcgcgtg aggccattga gcaagtactg
    13621 gcgaccggtg tcgatgtttt tctcgatatc gactggcagg gcgcgcagca aattcgccag
    13681 aaaatgccgc acgcgcggag tatctttatt ttaccgccgt ccaaaattga actggaccgc
    13741 cgtctacgcg gtcgcggtca ggacagcgaa gaggtcattg caaagcgtat ggcgcaagct
    13801 gttgcagaaa tgagccatta cgccgaatat gattatctga ttgtgaatga tgacttcgat
    13861 accgcgttga ccgatttgaa gaccattatt cgcgccgaac gtctgcgcat gagccgccaa
    13921 aagcagcgtc atgacgcttt aatcagcaaa ttgttggcag actgaacctg atttcagtat
    13981 catgcccagt catttcttca cctgtggagc tttttaagta tggcacgcgt aactgttcag
    14041 gacgctgtag agaaaattgg taaccgtttt gacctggtac tggtcgccgc gcgtcgcgct
    14101 cgtcagatgc aggtaggcgg aaaggatccg ctggtaccgg aagaaaacga taaaaccact
    14161 gtaatcgcgc tgcgcgaaat cgaagaaggt ctgatcaaca accagatcct cgacgttcgc
    14221 gaacgccagg aacagcaaga gcaggaagcc gctgaattac aagccgttac cgctattgct
    14281 gaaggtcgtc gttaatcaca aagcgggtcg cccttgtatc tgtttgaaag cctgaatcaa
    14341 ctgattcaaa cctacctgcc ggaagaccaa atcaagcgtc tgcggcaggc gtatctcgtt
    14401 gcacgtgatg ctcacgaggg gcaaacacgt tcaagcggtg aaccctatat cacgcacccg
    14461 gtagcggttg cctgcattct ggccgagatg aaactcgact atgaaacgct gatggcggcg
    14521 ctgctgcatg acgtgattga agatactccc gccacctacc aggacatgga acagcttttt
    14581 ggtaaaagcg tcgccgagct ggtagagggg gtgtcgaaac ttgataagct caagttccgc
    14641 gataagaaag aggcgcaggc cgaaaacttt cgcaagatga ttatggcgat ggtgcaggat
    14701 atccgcgtca ttctcatcaa acttgccgac cgtacccaca acatgcgcac gctgggctca
    14761 cttcgcccgg acaaacgtcg ccgcatcgcc cgtgaaactc tcgaaattta tagcccgttg
    14821 gcgcaccgtt taggtatcca ccacattaaa accgaactcg aagagctggg ttttgaggcg
    14881 ctgtatccca atcgttaccg cgtaattaaa gaagtggtga aagccgcgcg cggcaaccgt
    14941 aaagagatga tccaaaaaat cctctctgaa atcgaagggc gtttgcagga agcgggaata
    15001 ccgtgccgcg tcagtggtcg cgaaaagcat ctttattcga tttactgcaa aatggtgctc
    15061 aaagagcagc gttttcactc aatcatggac atctacgctt tccgcgtgat cgtcaatgat
    15121 tctgacacct gttatcgcgt gctcgggcaa atgcatagcc tgtacaagcc gcgtccgggt
    15181 cgcgtgaaag actatatcgc cattcctaaa gcgaacggct atcagtcgtt gcacacctcg
    15241 atgattggcc cgcacggcgt gccggttgag gtccagatcc gtaccgaaga tatggatcag
    15301 atggcggaga tgggtgttgc cgcgcactgg gcttataaag agcacggcga aaccagtact
    15361 accgcacaaa tccgcgccca gcgctggatg caaagcctgc tggagctgca acagagcgcc
    15421 ggtagttcgt ttgaatttat cgagagcgtt aaatccgatc tcttcccgga tgagatttac
    15481 gttttcacac cggaagggcg aattgtcgag ctgcctgccg gtgcaacgcc cgtcgacttc
    15541 gcttatgcag tgcataccga tatcggtcat gcctgcgtgg gcgcacgcgt tgaccgccag
    15601 ccttacccgc tgtcgcagcc gcttaccagc ggtcaaaccg ttgaaatcat taccgcaccg
    15661 ggcgctcgcc cgaatgccgc gtggctgaac tttgtcgtta gctcaaaagc gcgcgccaaa
    15721 attcgtcagt tgctgaaaaa cctcaagcgt gatgattctg taagcctggg ccgtcgtctg
    15781 ctcaaccatg ctttgggtgg tagccgtaag ctgaatgaaa tcccgcagga aaatattcag
    15841 cgcgagctgg atcgcatgaa gctggcaacg cttgacgatc tgctggcaga aatcggcctt
    15901 ggtaacgcaa tgagcgtggt ggtcgcgaaa aatctacaac atggggacgc ctccattcca
    15961 ccggcgactc aaagccacgg gcatctgccg atcaaaggcg ctgatggcgt gctgatcacc
    16021 tttgcgaaat gctgccgccc cattcctggc gacccgatta tcgcccacgt cagccccggt
    16081 aaaggtctgg tgatccacca tgaatcctgc cgtaacatcc gtggctacca gaaagagcca
    16141 gagaagttta tggctgtgga atgggataaa gagacggcgc aggagttcat caccgaaatc
    16201 aaggtggaga tgttcaatca tcagggtgcg ctggcaaacc tgacggcggc aattaacacc
    16261 acgacctcga atattcaaag tttgaatacg gaagagaaag atggtcgcgt ctacagcgcc
    16321 tttattcgtc tgaccgctcg tgaccgtgtg catctggcga atatcatgcg caaaatccgc
    16381 gtgatgccag acgtgattaa agtcacccga aaccgaaatt aatgttttat gaacccaaca
    16441 cgttatgcac gcatctgcga aatgctcgcc aggcggcagc ctgatctgac cgtctgcatg
    16501 gagcaggtcc acaaacctca taacgtttct gcgattattc gtaccgcaga tgccgttggc
    16561 gtacatgaag ttcacgccgt ctggcctggt agccgcatgc gcaccatggc ttcggcagcg
    16621 gcgggtagta acagctgggt acaggtgaaa acacaccgca ccattggcga tgccgtcgct
    16681 catctcaaag gccagggaat gcagattctg gcaacccatc tttctgataa cgctgtcgat
    16741 ttccgcgaaa ttgattacac tcgcccgacc tgcattttga tgggacagga gaaaacgggc
    16801 atcacgcagg aagcattggc cctggcggat caggacatca tcattccgat gatcggcatg
    16861 gtgcagtcgc tgaatgtttc cgttgcctca gccctcattc tttacgaagc ccagcgtcag
    16921 cggcaaaatg caggcatgta cctgcgtgaa aacagcatgt tgccggaagc agagcaacaa
    16981 cgcctgttgt ttgaaggcgg ctatccggtg ctggcgaaag tcgcaaaacg caaaggcctg
    17041 ccttatcccc acgtcaatca gcaaggcgag atcgaagctg atgccgactg gtgggctact
    17101 atgcaggctg cagggtaagt gccatgaaag gtcgcctgtt agatgctgtc ccactcagtt
    17161 ccctaacggg cgttggcgca gcacttagta acaaactggc gaaaatcaac ctgcataccg
    17221 tgcaggatct actcttacac cttcccctgc gctacgaaga tcgcacccat ctctacccca
    17281 tcggagaact actgccgggc gtttatgcca cggtggaagg cgaagtgctg aactgcaata
    17341 tctccttcgg cggtcggcgg atgatgacct gccagatcag cgacggttcc ggcatcctca
    17401 ccatgcgctt tttcaatttc agcgcggcaa tgaaaaatag cctggcgacg ggccgccgtg
    17461 tactggctta tggcgaagca aagcgcggta aatatggtgc ggagatgatc cacccggaat
    17521 accgcgtgca gggcgatctc agcacgccag aattacagga aacgctcacg ccggtttatc
    17581 caacaacgga aggcgtaaag caggccacgc tgcgtaaatt aaccgaccag gcgctggatc
    17641 tgctcgacac ctgcgccatt gaagaactcc tgccgccgga actgtcacaa ggaatgatga
    17701 cgctaccgga agcgttgcgc actttgcacc gcccgccacc gacgctacag cttagcgatc
    17761 tggaaaccgg gcagcatccg gcgcaacgtc gtctgattct ggaagaactg ctggcgcaca
    17821 acctcagcat gttagcctta cgtgccggag cacagcgttt tcatgcccag ccgctgagcg
    17881 ccaatgacac gctgaaaaat aaactcctcg ccgccttacc gttcaagcca acgggcgcac
    17941 aggcacgcgt agtggcggag atcgagcgcg atatggcgct ggatgtgccg atgatgcgtc
    18001 tggtgcaggg cgatgtaggt tccggtaaaa cgctggtcgc cgccctcgcc gcgttgcgtg
    18061 cgattgccca cggcaaacag gtagcattga tggcaccaac cgaattactc gccgagcagc
    18121 acgccaataa cttccgcaac tggtttgcac cgctcggtat cgaagtgggc tggctcgccg
    18181 gtaagcagaa aggtaaagca cggctggcac agcaggaagc catcgccagc ggtcaggtgc
    18241 agatgattgt cggtacacac gccatcttcc aggaacaggt gcagtttaac ggcctggcgc
    18301 tggtgattat cgacgaacag catcgttttg gcgtgcatca gcgtctggca ttgtgggaga
    18361 aaggccagca gcagggcttc catccgcatc agttgatcat gaccgccacg ccgatccccc
    18421 gcacgctggc aatgactgcg tatgccgatc tcgatacctc ggtgatagat gagctgccgc
    18481 caggccgcac gccagtgact acggtcgcta ttcctgatac ccgccgtacc gacatcattg
    18541 accgcgtgcg ccacgcctgc ataactgaag gtcgtcaggc atactgggtt tgtacgttga
    18601 ttgaagagtc ggaattgctg gaagcgcagg cggcggaagc tacctgggaa gagttgaaac
    18661 tggcgctacc agagttgaac gttggcctgg tacacgggcg gatgaaacct gtcgagaaac
    18721 aggcggtgat ggcgtcgttt aaacaaggtg agctacacct gctggttgcc acaaccgtta
    18781 ttgaagtcgg cgttgatgtg cctaacgcca gtctgatgat tatcgaaaac ccggagcgtc
    18841 tgggtctggc gcagttacac cagcttcgcg ggcgtgtagg tcgtggtgcg gtggcttctc
    18901 actgcgtgct gctctacaaa acgccgctct ccaaaaccgc gcaaattcgg ctgcaagtgc
    18961 tgcgcgacag caacgacggt tttgtgattg cacagaaaga cttagagatt cgcggaccgg
    19021 gtgaactgtt aggtacgcgt cagacgggta atgctgagtt taaagtggcg gatttactgc
    19081 gcgatcaggc gatgatcccg gaagttcagc gcctggcgcg acatattcac gaacgttacc
    19141 cacaacaagc aaaagccctg atagaacgct ggatgccgga gacggaacgt tactccaacg
    19201 catgaccaga ctcatcacaa gacaagtcac gcagtaaatc ttcaatctgg atagtgagga
    19261 tatccattgc ctcatacgca tcctgtgcaa ttcgcgccgc cattgcgcct tccggttcac
    19321 cacgcagaat ttcagccgct gcctgtacgc cagccacaat catttcaggt gttaacattt
    19381 ggcgaagaaa agcgcgatta tcagaggacc agccatcttt atggcctaat ggatgccccc
    19441 agcctatcca ttcttgcgac cataacggat tggcttctat gggataaatt agatgcgcgg
    19501 catactgaat agcacacacc gcttttcctg gcgtatccag cgcccaccag cgcgaccaga
    19561 gagaatgaat taacggcgcg gccccaccca gagtattgaa caccgccagc cagcaaagaa
    19621 cgttattgaa accacgctcg ttatccatac gctgcaacgc ggtgtccagt aaaaattgcc
    19681 gtacctgctg gcgttgggaa gcattcatca tctcctgcca gagatagggc cgacacaatg
    19741 ccttcattaa ggattcttcg aagtgatcac cataaccgcc agctaaaacg gtttcatgcc
    19801 attgcgccag gcagagcgga aacagatagc gaaacaactc tatttgcatc ggttcgttat
    19861 aaacgagatt gagtacatag taagcactca aatcccatac gctgatctgc tgccactctt
    19921 tagccgccag cgccttgagt tcggcatctg cgtaatcaaa ttccgtttcc catacacaga
    19981 gcactggcgg ctcatcacca aaaatatggc gacagtgttc acgtagcgtt tccggttcct
    20041 tccgacgctt tttcattttt aaccggcaaa aatcggcaac attaaataca gcttaatcac
    20101 cagcgcattg acgatatcga taaagaacgc accgaccatc ggcaccacca aaaacgccat
    20161 gtgcgacggg ccaaagcgtt cagtgatcgc ctgcatgttg gcgattgccg ttggcgttgc
    20221 accgaggcca aaaccacagt gacccgcagc cagcactgcc gcatcgtagt ttttgcccat
    20281 catgcgccag gtaacgaaga tggcatacaa cgccatgaag atggtctgta ccaccagaat
    20341 cgccagcatc ggcagcgcca gcgaagccag ctcccacagt ttcagcccca tcagcgccat
    20401 cgccaggaac aacgacaggc ttacgttacc cagcacggat accgcacgct caaagacgcg
    20461 gtaaaagccc atcattgaca gaccgttgct cagaatcacg ccaacaaaca gtacacagac
    20521 gaaggtcggc agttcaaaag cagtgccagc caaaagttgc gcaacaattt tccccaccgt
    20581 caggcagata gcaatcagcg cgatagtttc aatcagcacc agcgaggtga tcatgcggcc
    20641 cacatccggc ttttcaaacg ccgtcgggac ttcctggtca tccggaatac cgttcggcgt
    20701 ggtggagtgt ttcaccagat agcgcgccac cggaccgcca atcaagccgc ccagcaccag
    20761 accgaacgtt gcacaggcca tcgccacttc cgtcgcattg gtgaagccat aacgttcaat
    20821 gaacaattta ctccacgcag cgcccgtacc gtgaccgccg gaaagcgtaa tagaaccggc
    20881 caacagcccc atcagcggat caagccctaa caggctagcc ataccaatgc caatggcatt
    20941 ttgcatcacc aacagaccaa caaccacaat caagaagatg ccaaccacac gcccaccggc
    21001 acgcaaactg gcaatgttgg cgttcaggcc aatggtggcg aagaaagcca gcattaacgg
    21061 atcgcgcagg gacatatcaa agttgacttc ccagcccatg ctttttttca gtactagtag
    21121 cgccagcgcc accaacaaac cacccgcaac aggttccggt atggtgtatt tcttcaaaaa
    21181 ggagacggaa tggaccaact tacgcccgag cagcaacgtc agcgttgcgg caacaagcgt
    21241 tgctaaagta tcgagatgaa acatagttac tcctttgtat ccgcatcact ctttcatact
    21301 cgtcatactt catcgcgcag tggcaacttc atgaactatg acgggtatcg agttatgctt
    21361 tcctgggtca aattctcagc tgttaatgaa caaatggcat ggattttaag cagaaaaata
    21421 ccaaaagtta tataaaaaag tgcatttatc tcatttaaat atttagatga ggatgtggca
    21481 aacgtttgct tttctctttc ggtcagctaa aatgcccgct ttgctaccac gggattgttt
    21541 tcgatgtctg tttccaccct cgagtcagaa aatgcgcaac cggttgcgca gactcaaaac
    21601 agcgaactga tttaccgtct tgaagatcgt ccgccgcttc ctcaaaccct gtttgccgcc
    21661 tgtcagcatc tgctggcgat gttcgttgcg gtgatcacgc cagcgctatt aatctgccag
    21721 gcgctgggtt taccggcaca agacacgcaa cacattatta gtatgtcgct gtttgcctcc
    21781 ggtgtggcat cgattattca aattaaggcc tggggtccgg ttggctccgg gctgttgtct
    21841 attcagggca ccagcttcaa ctttgttgcc ccgctgatta tgggcggtac cgcgctgaaa
    21901 accggtggtg ctgatgttcc taccatgatg gcggctttgt tcggcacgtt gatgctggca
    21961 agttgcaccg agatggtgat ctcccgcgtt ctgcatctgg cgcgccgcat tattacgccg
    22021 ctggtttctg gcgttgtggt gatgattatc ggcctgtcgc taattcaggt tgggttaacg
    22081 tccattggcg gcggttacgc agccatgagc gataacacct tcggcgcacc gaaaaatctg
    22141 ctgctggcag gcgtggtctt agccttaatt atcctgctta accgtcaacg taacccttac
    22201 ttacgcgtgg cctcactggt gattgcgatg gcggccggat atgcgctggc gtggtttatg
    22261 ggcatgttgc cagaaagcaa cgaaccgatg acgcaagaac tgattatggt gccaacgccg
    22321 ctctattacg gtcttggcat tgaatggagt ctgctgctgc cgctgatgct ggtctttatg
    22381 atcacttcgc tggaaaccat tggcgatatc acggcgacct ctgacgtttc cgaacagcca
    22441 gtgtccggtc cgctgtacat gaaacgcctg aaaggtggcg tgctggcaaa cggcctgaac
    22501 tcgtttgttt cggcggtgtt taacaccttc ccgaactcct gcttcgggca aaacaacgga
    22561 gtgatccagt tgactggtgt tgccagccgc tatgtcggtt ttgtcgtcgc gctgatgttg
    22621 atcgtgctgg gtctgttccc ggcagtgagc ggttttgtac aacacattcc agaaccggtt
    22681 ctgggcggcg caacgcttgt aatgtttggc accatcgccg cctccggtgt gcgtatcgtt
    22741 tctcgtgagc cgctgaaccg tcgggcgatt ctgattatcg cgctgtcgct ggcggttggt
    22801 ctgggcgtgt ctcagcagcc gctgattttg cagtttgccc ctgaatggct gaaaaacctg
    22861 ctctcctccg ggatcgccgc gggcggtatt actgccatcg tgctgaatct gattttccca
    22921 ccagaaaaac agtaattcac tggcggcggt aagataatta ccgccgcagt ctcacctctt
    22981 tcaccattcc cgccttgagg attgcgcgta aatcgtgcat aactccctta tgtgcatttc
    23041 acgggatgga agaccatgaa atttattggg aagctgcttc tctacattct catcgctctg
    23101 ttagtggtga tcgctggcct ctattttctt ctgcaaaccc gctggggagc agaacatatc
    23161 agcgcatggg tttccgagaa tagcgactat catctggcct tcggggcgat ggatcaccgt
    23221 ttttccgcgc catctcatat cgtgctggag aacgtcacgt ttggccgtga tggccagccc
    23281 gcgaccctgg tggcaaaaag tgtcgacatt gcgctaagca gtcggcaact gaccgaacca
    23341 cgccatgtcg ataccatcct gctggaaaac gggacgctga atctcaccga ccagaccgcg
    23401 ccgctaccgt tcaaagccga tcgtctgcaa ctgcgtgata tggcgtttaa tagcccgaat
    23461 agcgaatgga aactgagcgc gcagcgggta aatggcggcg tagttccgtg gtcaccaaaa
    23521 gccggtaaag tgctgggtac gaaggcgcag attcagttta gtgccggatc gctttcgctc
    23581 aatgatgttc ctgccaccaa tgtactgatt gaaggcagta ttgataacga tcgcgttacg
    23641 ctgactaacc tgggtgccga catcgcccgc gggacattaa ccggaaacgc gcagcgtaac
    23701 gccgacggca gctggcaagt ggaaaacctg cgcatggcgg atatacgtct acaaagcgaa
    23761 aaatcgctaa ccgacttctt tgcgccatta cgctctgtcc cgtcgttgca gattggtcgc
    23821 ctggaagtga tcgatgctcg tttgcaaggt ccggactggg cggtgaccga cctcgatctc
    23881 agcttgcgca acatgacctt cagtaaagat gactggcaga cacaggaagg caaactgtcg
    23941 atgaacgcta gcgagttcat ttatggttcg ctgcatttat ttgacccgat tataaacgcg
    24001 gaattttccc cgcagggcgt agcgctgcgc cagttcacca gccgctggga agggggtatg
    24061 gtcagaacgt cagggaactg gctgcgtgac gggaaaacgt tgatccttga tgatgtggca
    24121 attgccgggc tggaatatac cttgccgaaa aactggcaac agttgtggat ggaaacgaca
    24181 cccggttggt taaacagcct gcaactgaag agatttagcg ccagccgcaa tctgatcatt
    24241 gatatcgacc ctgacttccc gtggcagctc accgcgctcg atggttacgg tgccaacctg
    24301 acgctggtta ccgatcataa atggggcgtc tggagtggct cggcgaatct gaatgccgcc
    24361 gccgcgacat tcaatcgtgt tgatgttcgt cgcccgtcgc tggcgctgac cgccaacagc
    24421 agcacggtga atatcagcga actgagtgca tttactgaaa aaggcattct ggaagccacc
    24481 gccagtgttt cacaaacgcc acaacgtcag acacatatca gcctgaatgg acgcggtgtg
    24541 ccggtgaata ttttgcaaca gtggggatgg cctaaattac cgttgactgg cgacggcaat
    24601 attcagctta ccgccagtgg cgatattcag gccaatgtcc cgttgaaacc tacggttagc
    24661 gggcaattgc atgccgtgaa cgccgcaaag cagcaagtga ctcaaaccat gaatgctggc
    24721 atcgtttcca gcggtgaagt tacatcgacg gagccggtgc ggtaaacatc aatctcgccg
    24781 ggtacgactc agacgtaccc ggcattccat caatagatta caacgtaatt gtcagcgcat
    24841 tcccttgagg cttcaccacc agcccctgct cactttcagc ctgcgaaccg tcttgcagac
    24901 catttacttt cacaacattg cgcaggcaca gcgtccagtt cttcgcctcg cccgcaccag
    24961 tcacagtaat cgtgttgcca gtacgtgctg cttttaaagt aaagatcacc gatccgtcag
    25021 tagcgggcac ttcacagacg gcttcatgcc cgtcttgcag attaaagagg tggaatgccg
    25081 tgccttcgtg ccacacgtaa tcgggacgtt gatcgttgtt gcccagcgcc agtagggtgt
    25141 tatcacgcac ataaatgggc agactcagga agctgtgctg ctgtttatgc cagcgactac
    25201 cgtcgagctc atcgttgtgc cacaggtgtg tccagcgacc ttccggcagg tagaactgca
    25261 catcgcccgc ttcagtgaac accggcgcaa ccatcacgtt gtcgcctaac atgtattgac
    25321 ggtcaaggta atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg
    25381 gcgtaccccg cgcgttcgca cgcgcagctt cacgatacag atacggcatc atgcggcatt
    25441 tcagttgcgt gaagaagcgc accacatcac aggactcatc atcgtaggcc cacggcacac
    25501 gataagattt gctaccgtgt aaacggctat ggctggagag caaaccaaac gcgcaccagc
    25561 gtttgtaaac gtgcgccgga gcggtatttt caaagccgcc gatatcgtgg ctccagaagc
    25621 caaaacctga aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt
    25681 tagcgtaaca atcgccaccc cagtgtaccg ggaatttctg cgcaccgacg gaggccgagc
    25741 gggcaaacaa gacagcttct tcctcaccaa cggtgtcctt gagcacgttc cacaccagtt
    25801 cgttgtagat gtacgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact
    25861 gaacatcagt tgggatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga
    25921 tcagaccttt cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa
    25981 tcgccagacc tggctgccat ttatcccact gccatagcga accgtccggg cgtttgagta
    26041 aatagccttt ctcttgtaac tctttaaaga cgggggattt ttgaccgata taggggttaa
    26101 tccagacgca gattttcagt cctttcgctt tcaggcggcg gatcatccct tccgggtcag
    26161 ggaaagtcag cgggtcccac tcaaaatcgc accactggaa ggctttcatc cagaaacagt
    26221 caaagtggaa aacatgcagc ggcagattgc gttccgccat accatcgata aagctgttta
    26281 ccgtcgcttc gtcgtagttg gtggtaaatg aagtggttag ccacaggccg aaggaccacg
    26341 cgggcggcag cgccggacga ccagtaaagc gggtataacg atcaagtacc gctttcggcg
    26401 tcgggccgtc gataacaaag tattcgagat attcactctc aacgctgaac tgcactttgg
    26461 agactttctc cgatcccact tcaaaagaga cacactgggg atgattgacc agtaccccat
    26521 aaccacggtt agtcatgtag aacgggatat ttttatacgc ctgttcagta cttgtgccgc
    26581 cgtcccggtt ccaggtctct accgtctggc cattgcgcac cagggcagta aagcgctctc
    26641 ccagaccgta aactgtttcg ccaacgccaa gatcaagccg ctcaaacata taattgcgtt
    26701 gattattcgt gtcctgcacg tagccattat ttttcacctg actaccggta atacgttcgc
    26761 cgttgcgcag aaaatccagt gaccagaact cacctttgct gacacgcgcg cttaagttgc
    26821 cacttttaaa ctcagcataa cgttctgtgt tttcgattgt gaccttcacg tcctgcaaaa
    26881 tattgagcgg ataatgagga ccgttattca gcgccccctg aaaatgctca atccgcacac
    26941 cgacaatacc ttcctgtggg gagaaaaagc gcaacgtaaa taaaggagta tcaagctgcc
    27001 aggtacgttc acgcacatca cggggggcag catagaccac catttcatta tcctgctgtt
    27061 caacctcgaa cacctgaagc gggtgaatca aattgaggcc aggttgaatc aaccagtttc
    27121 catcgctaat tttcattgcc gttccttagt tctgcacttc ttgagaggtg aattgttgct
    27181 ggcaacggcg tttaccctga gccagctgtt ccataacggt tttcagattg tgcgtcgtga
    27241 gtgagtagta gcgtttagcg ataatcgcgc tcagcaaata acagatcgcc ggaacaatcg
    27301 tgaatagcgc aataatgatg ctaatcgtgg cgctgttctg cgctttttct gccgcatcat
    27361 atccgccata agccagcatc cagccgataa gagcgccgcc gaaggccaac cccagtttga
    27421 gcacaaacag cgtgccagca aaactgatcc cggtcaggcg tttaccattg caccactcgc
    27481 cgtagtcgac ggtatcggac atcattaccc actggatagg tgtcaccagt tgatgcaaca
    27541 caccaatcac gaagatgaag acaaacatag tgatgctggc ctgcatggga acaaagaaca
    27601 tcgcgaggct aatcactgcc agcagggcgt tcgtccacca gaagatagtg actttacatt
    27661 tccagtcggt cagaggtttt gccagtgcgg aaccaatcag gttacccacg caataagtgg
    27721 tgagaaaagc gacaaacact tccggcgtgc ccaaaatcca tgtgacgtaa tacatcatcg
    27781 ccccaccgcg tacgcacacc gccaggatat tgaaaatggt tagtaaaccg acaatccgcc
    27841 actggtcgtt ttgccagata tcacgtaaat cttcccgcat agacgttgtt gtaggtggtg
    27901 cttcaacgcg ttctttagtg gtgaagaaac aaaatgccag catcatgaat gccaccacgg
    27961 aaaggaccgc gataccgccc tggaaaccga gtggtttatt atcaccgcca attaaattaa
    28021 ccagtggcat catcagaaca gtagaaagca tgcctcccgc ggtcgccagc acaaaacgcc
    28081 aggattgcag cgagatacgc tgagtcgggt cattggtgat tacaccaccc aatgcgcagt
    28141 aagggatatt gacgacggta taaagtaagg taagtagggt gtaagtaatt gctgcataga
    28201 tcattttgcc gttcatactg agatctggcg tgctataggc cagtacacag acgatcccga
    28261 atggcagtgc gccaaacagt acccacggac gaaatttacc ccagcgagag cgcgttcgat
    28321 cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag tgcacgagcg accaaaaaca
    28381 tggttccgac aaatccggca ggaatgccaa aaatatcggt ataaaagaac atcatatata
    28441 acattacgtt atcgaaaata atgtggctgg cggcgtctcc catgccataa ccaattttct
    28501 ctttaacgga caacacttca ctcttcatct gcttttcctt gatgctatag gggtgctgag
    28561 accgataacg cattttgtaa accatccctg tgagagcgaa tattccgata tctggttatt
    28621 aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa ttccgctcat atatcattgt
    28681 aaaatatggg ttttatatga actataatgc tttcgtgata atacgctgcg tgtattaggc
    28741 ggaaaaaact gatctggggg atgtagaaac tcaaggaagt agctataatg cgccccgcct
    28801 ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt gaggcggctg gacttcaaat
    28861 ccagttgggg ccgccagcgg tcccgggcag gttcgactcc tgtgatcttc cgccaaaatg
    28921 cctctcctga cgttctccaa ccccttgtaa tactgtcaat ccagatattt tctactgctc
    28981 aatattcaat cggcttcagg aatacctggt ctaaggtttt gtgatggacg atgagcggct
    29041 gaagaatccg cctgtcggtt cttctgtcct cgaacgcatc cgcgatattc acgccagtat
    29101 gcgccgtgtt tatttgcagg tgatttgccc ccctgctgac tctcagccaa ctgtatcgcc
    29161 attaaggtga agatgttgtt ttactcgccc cagaacaccc ttttttatac gactattcta
    29221 tttctttaac cgctggcgaa aaaaactgac tataaacttc accaataatc agactgccat
    29281 caagattaat ttgttcattt aatatatcaa atcaacttgt
//
LOCUS       DAFAVF010000048        28009 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFAVF010000048 DAFAVF010000000
VERSION     DAFAVF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.048"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..863
                     /locus_tag="LGK42_002025"
     CDS             <1..863
                     /locus_tag="LGK42_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK2658276.1"
                     /translation="NETPYNIPLLINENVISSGISLISLWHTYADEHYRVIWPRDKKK
                     PLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHCEGLVWEQYWME
                     FTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVAFLTKIIYQWIC
                     LMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLRRLFLRYTGKTP
                     KEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTD
                     QHPTDASPHN"
     gene            complement(860..1036)
                     /locus_tag="LGK42_002026"
                     /pseudo
     CDS             complement(860..1036)
                     /locus_tag="LGK42_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(1036..1674)
                     /locus_tag="LGK42_002027"
     CDS             complement(1036..1674)
                     /locus_tag="LGK42_002027"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658277.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            complement(1674..3290)
                     /locus_tag="LGK42_002028"
     CDS             complement(1674..3290)
                     /locus_tag="LGK42_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK2658278.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3586..4302
                     /locus_tag="LGK42_002029"
     CDS             3586..4302
                     /locus_tag="LGK42_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2658279.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4299..5960)
                     /locus_tag="LGK42_002030"
     CDS             complement(4299..5960)
                     /locus_tag="LGK42_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBK2658280.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6157..6585)
                     /gene="ibpB"
                     /locus_tag="LGK42_002031"
     CDS             complement(6157..6585)
                     /gene="ibpB"
                     /locus_tag="LGK42_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBK2658281.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6697..7110)
                     /gene="ibpA"
                     /locus_tag="LGK42_002032"
     CDS             complement(6697..7110)
                     /gene="ibpA"
                     /locus_tag="LGK42_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBK2658282.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7104..7176)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7416..7748
                     /locus_tag="LGK42_002033"
     CDS             7416..7748
                     /locus_tag="LGK42_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBK2658283.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7750..8964)
                     /locus_tag="LGK42_002034"
     CDS             complement(7750..8964)
                     /locus_tag="LGK42_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBK2658284.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9065..10129
                     /gene="cbrA"
                     /locus_tag="LGK42_002035"
     CDS             9065..10129
                     /gene="cbrA"
                     /locus_tag="LGK42_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBK2658285.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(10126..11463)
                     /locus_tag="LGK42_002036"
     CDS             complement(10126..11463)
                     /locus_tag="LGK42_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658286.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11538..12686)
                     /gene="dgoD"
                     /locus_tag="LGK42_002037"
     CDS             complement(11538..12686)
                     /gene="dgoD"
                     /locus_tag="LGK42_002037"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBK2658287.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12683..13300)
                     /gene="dgoA"
                     /locus_tag="LGK42_002038"
     CDS             complement(12683..13300)
                     /gene="dgoA"
                     /locus_tag="LGK42_002038"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBK2658288.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13284..14162)
                     /gene="dgoK"
                     /locus_tag="LGK42_002039"
     CDS             complement(13284..14162)
                     /gene="dgoK"
                     /locus_tag="LGK42_002039"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBK2658289.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14159..14848)
                     /gene="dgoR"
                     /locus_tag="LGK42_002040"
     CDS             complement(14159..14848)
                     /gene="dgoR"
                     /locus_tag="LGK42_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBK2658290.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15126..15782
                     /locus_tag="LGK42_002041"
     CDS             15126..15782
                     /locus_tag="LGK42_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658291.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15828..16640)
                     /gene="yidA"
                     /locus_tag="LGK42_002042"
     CDS             complement(15828..16640)
                     /gene="yidA"
                     /locus_tag="LGK42_002042"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK2658292.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATISLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16755..17153)
                     /locus_tag="LGK42_002043"
     CDS             complement(16755..17153)
                     /locus_tag="LGK42_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBK2658293.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17393..19807)
                     /gene="gyrB"
                     /locus_tag="LGK42_002044"
     CDS             complement(17393..19807)
                     /gene="gyrB"
                     /locus_tag="LGK42_002044"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK2658294.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVRTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19836..20909)
                     /gene="recF"
                     /locus_tag="LGK42_002045"
     CDS             complement(19836..20909)
                     /gene="recF"
                     /locus_tag="LGK42_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK2658295.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMVDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20909..22009)
                     /gene="dnaN"
                     /locus_tag="LGK42_002046"
     CDS             complement(20909..22009)
                     /gene="dnaN"
                     /locus_tag="LGK42_002046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK2658296.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22014..23417)
                     /gene="dnaA"
                     /locus_tag="LGK42_002047"
     CDS             complement(22014..23417)
                     /gene="dnaA"
                     /locus_tag="LGK42_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK2658297.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23714..23794
                     /locus_tag="LGK42_002048"
     CDS             23714..23794
                     /locus_tag="LGK42_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658298.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            24024..24164
                     /gene="rpmH"
                     /locus_tag="LGK42_002049"
     CDS             24024..24164
                     /gene="rpmH"
                     /locus_tag="LGK42_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK2658299.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24181..24540
                     /gene="rnpA"
                     /locus_tag="LGK42_002050"
     CDS             24181..24540
                     /gene="rnpA"
                     /locus_tag="LGK42_002050"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK2658300.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24504..24761
                     /gene="yidD"
                     /locus_tag="LGK42_002051"
     CDS             24504..24761
                     /gene="yidD"
                     /locus_tag="LGK42_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK2658301.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24764..26410
                     /gene="yidC"
                     /locus_tag="LGK42_002052"
     CDS             24764..26410
                     /gene="yidC"
                     /locus_tag="LGK42_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK2658302.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26516..27880
                     /gene="mnmE"
                     /locus_tag="LGK42_002053"
     CDS             26516..27880
                     /gene="mnmE"
                     /locus_tag="LGK42_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK2658303.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6805 a         7526 c         7124 g         6554 t
ORIGIN      
        1 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
       61 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
      121 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
      181 gtgggaaaat tcttttaaag aatggcgaac aaataacact gcatggcaac tgtattatat
      241 tcttaaagcc aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact
      301 ggatggaatt tacccccacc agcatgatgg atattcccgt tggtcagcaa agcgttattt
      361 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
      421 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
      481 gtcttatgta cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
      541 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
      601 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
      661 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
      721 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
      781 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
      841 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
      901 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
      961 cctaccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
     1021 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
     1081 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
     1141 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
     1201 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tccaggcgtt
     1261 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
     1321 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat
     1381 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc
     1441 aacgtcgctg aatgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
     1501 ctctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcac catcgttatc
     1561 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
     1621 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
     1681 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
     1741 tcacgcagct gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
     1801 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
     1861 agtgcaatac gtaaacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
     1921 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc cgcggtggtt
     1981 tggcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
     2041 cccggcgtgc acatgttgaa ctgcagaatc agtgtgcgga aaaccacgaa gtacagcagg
     2101 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
     2161 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
     2221 ccaaagagat acatcacggt cgacattgag gccgcaagca cagcgtgtac cgcaaacagc
     2281 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg
     2341 gttaaggttg cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg
     2401 aagtacatcg ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca
     2461 aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga
     2521 tgctgcgccc agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt
     2581 ccgtagatga agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc
     2641 cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt
     2701 acttttggcc agccgagcaa cgtcagccag gcacagggga tcatcaccag gaaggcgata
     2761 atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac
     2821 agacggttat gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta
     2881 tcgagggttt taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg
     2941 aaatcgacgc cgaagtagct tccccaggtc attcccatcg cgttgatgaa atagttccag
     3001 gtcaggaaac tcaccatcac cgccagacaa gcacgcccct gcgcttgctt agcaaggcca
     3061 atgggtaaac cgacagcaaa aatcagcggc atattacgga acaccgtcca accgccctct
     3121 tcaataatgt gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca
     3181 aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc
     3241 agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc
     3301 tcttttctca attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg
     3361 ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc
     3421 ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg
     3481 tacacagcat cgaacacatc tttaaaaaaa agatgttttt tcaatcgatt aagcagaact
     3541 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagatc tagagatgat ctacaaaagc
     3601 attgcggagc ggttaagaat tcgacttaat tctgcagatt tcacgctaaa tagccttctt
     3661 cccggtgaaa aaaagttggc ggaagaattt gcggtatcgc ggatgaccat ccgtaaagcc
     3721 attgacctgc tggtagcgtg gggattggtg gtccgccgcc acggcagcgg cacttacctg
     3781 gtgcgcaaag atgtactgca tcaaaccgcc agcctgaccg ggctggtgga ggtgttaaaa
     3841 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
     3901 gtcattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
     3961 cgttttgtgg aagggaaacc gctgatgctg gaagacagct atatgccggt aaaactgttc
     4021 cgtaatcttt cgctgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
     4081 gggattttga ttggcggtaa ttatgaaagc ctgacgccag tgctcgccga tagactgctg
     4141 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
     4201 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaataccag
     4261 gtggagtacc atttacggcg actccacccg gaaaagagtt aaccgatact ccagaagagc
     4321 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
     4381 taagagagcg ccgccgcacc gctggttgga tgaagattat tagcaaacgc cagcgccgga
     4441 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
     4501 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
     4561 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
     4621 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
     4681 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
     4741 agcggaccgc ccgccagccc cagtttcaac gcggccggga acccaggcac aaagacggga
     4801 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
     4861 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
     4921 cccaccagat tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
     4981 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
     5041 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
     5101 gtgcctttcg tggacagcga agtatcgacc tcctgaccaa tcaccagttg cgcattatgt
     5161 aaatccgctg gctgacccac cagatgcagc aaatcgccca gttgaataat ggtatctggc
     5221 gaaggaactt ttagggtttc ttcgcgtttc agacgcgagc agataatttt gtcgccgttg
     5281 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
     5341 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
     5401 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
     5461 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
     5521 cccaggtcgc gcaaaatctg ctgccctgcc cccagcgctg gcgtattggt aaccgcaccg
     5581 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
     5641 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
     5701 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tagtataaac gaacaggatc
     5761 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
     5821 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
     5881 cgaaatttga cattgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
     5941 gttaatgcta tatcactcat cgtccatttc tcttgagata acatttttaa ttgctacgga
     6001 ttatggctga atcggtgaag cagatttgcc ttatagcgca cattatcacg ggaaagaacg
     6061 tgccgaaata tcttaaacag gcgaaattta acgcattgtt gaagaagaaa gacaaaaaaa
     6121 gccccaccag aatggcgggg caaagagaat agctagttag ctatttaacg cgggacgttc
     6181 gctgatagcg atacgctgcg ctgcgatggg ttcaggctca ttacgaatta aatcaatatg
     6241 cagtaaaccg tttacgaagg ttgcgccaga gacttccata ttttcagcca gcgtaaagct
     6301 caggctaaat ggctgattca taagcccttg atgcagccat tttttctctt cttttggctg
     6361 ctccggcgtg ccttttacgc tcaggcgcgt accttccagt tgaatctcta aatcttcctg
     6421 acggaaacct gccagcgcaa gggtaatgcg gtagtggtta tcgtcgcttt tctcaatgtt
     6481 gtacggcggg aagctctggc tttcaccggc gttttgcagt gcgttggcca gtttgtcaaa
     6541 accgatccat tgacgcatca gtggggataa atcgaagtta cgcatagtca tttctccttc
     6601 taagaagcga gtaagtacct gcaaatccga agattcgcat atgctccctg acggcgagca
     6661 tggagatgtc aggccgcgcc aggcggcctt aaggaattag ttgatttcga tacggcgcgg
     6721 ttttttcgct tccggaatca cgcgttcgag atcgatatac agcaaaccat ttaccaggtt
     6781 agcgccacga acatgaatgt tctcagctaa ctggaatttg cgttcaaagt tgcgttcagc
     6841 gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg gcgtgagcac ctttcaccac
     6901 cagcagatta tcctgggcgg taatttccag ttcgctctca gcaaaaccag ccacagcgat
     6961 agcaatgcgg taatggtttt cgtctaccag ttcaacgtta tacggagggt agccgccatt
     7021 actctggctc tggttgtttt ctaagtggtt aaacaaacgg tcaaatccaa tagcagaacg
     7081 gtaaagcggg gataaatcaa agttacgcat aatcaatagc tcctgaaatc agcgagaatg
     7141 taagaccttc cacaatggac aggtcaggta gccagaacac ccatcaggcg cgtcctcatc
     7201 ggctacgatg taaaaatggg tctggaaatg aacttttcaa gccttatcag cggacttttt
     7261 tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt aaagtgcgat
     7321 agccgccaca gtgcgacgta atgatgatgt tattttcagc aagggatcgc tataactcat
     7381 caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg ttgttagcgt
     7441 tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg tcccataccg
     7501 gagggaaaga aggaacgtat cccggcaccc gcgccagcgc aaccatgatt ggcgacgatg
     7561 aaaccaactg gggcactaag tcgctggcga ttctcgatat gccgtttacc gctgtgatgg
     7621 acacgctttt actgccgtgg gatgtgttcc gcaaagacag ctcggtgcgc tcgcgcgttg
     7681 aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc agaatgcctg
     7741 ataactgatt tagcgatcag tttcggtgat ccacagctgg ccgccttcca tccacgccag
     7801 ccactgacca tccggcgaga agaccacggc gtccgcagaa ggtggattgg cgtggttttc
     7861 ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa gcaattcgat tatccagcac
     7921 aaagcccaac cattctcctg acggatgcca gttaaatgca gactgaatat ccgttttgtt
     7981 atgggttaac tggcgcggct cgccgccctg tggcgagata agccacagtt gcacaatgcc
     8041 gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc gcacccagtg
     8101 gcgcgggacg ttgactaacc ccggataagc ccgatggtgg gtaaaggtta aacgtcgctg
     8161 cacgacgccg cgcggtggcg cgggcagggt ggtttccgtt ccacttaacg gcgcatcacc
     8221 tgccgctttc cagccagctt catcttgcgg taactcaacg ataaacagct ccggcacttt
     8281 ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc
     8341 ttcttcataa gcacgattga tttcatcact gccaggctgc ggtgtgggcg tggttttgct
     8401 caccagtacg caccagtggc taccgctgta ttcacgcgga tgctgctttt gtacgttgac
     8461 cgggccaaac ggtgcggcaa caccgacgtt tcgcaaatcc agcgccggat cgagttcatg
     8521 cattacatgg tcgttatagg taaagctcac cctttctccg ttcgggctaa agacatgcac
     8581 atggctgccg ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag
     8641 attgctcacc ttgccgcctt cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca
     8701 tgtttcatca ggattttccg ggccatggat gaaaacatat ttctctgact tcggatgaac
     8761 ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga cctcgccggt
     8821 atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc
     8881 aaataccagc cactggctgt cgggcgtcca ggtattggta ttggtgagca ggtgattacg
     8941 gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacgggtc ccgccatcat
     9001 acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga atgtgagtga
     9061 aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcaggat cggcgttggc
     9121 gcgaaaatta gcaggcaaaa tgcaggtgat cgcgctggat aaaaagcacc agtgtggtac
     9181 tgaaggtttc agcaaacctt gcggcggtct gctggcaccg gacgcgcagc gatcttttat
     9241 tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat ccacagattt tcagcgtcaa
     9301 aactgtcgac gtcgccgcat cgctcacgcg taactaccag cgaagctata tcaatattaa
     9361 tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc gccagcgttg aggtttatca
     9421 cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa tggcatgtca tttttcgtgc
     9481 agacggctgg gagcaacata ttactgcccg ctatctggtc ggtgccgatg gcgcaaactc
     9541 gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg ctatccagca
     9601 gtggttcgca gagaaacatc cggtgccgtt ctactcctgc atctttgata attcgataac
     9661 taactgttat tcatggagta tcagcaaaga cggttatttt atctttggcg gtgcttatcc
     9721 aatgaaagac ggtcagacgc gtttcacgac gctgaaagag aaaatgagcg cctttcagtt
     9781 ccagtttggt aaggcggtga aaagcgaaaa atgcacggtg ctgtttccct cacgctggca
     9841 ggattttgtc tgcggtaagg acaacgcctt tctgattggc gaagcggcag gatttatcag
     9901 cgccagttcg ctggagggga ttagctatgc gctggatagc gcagacattc tgcgttcggt
     9961 gttactgaag cagccagaga agctcaatac cgcttactgg cgcgccaccc gcaaactgcg
    10021 tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg ctttaagaaa
    10081 gtggattatg cgcagtggta tggcgcatat tccacagttg aaagattagc caacgcgctt
    10141 cacatcgccc accagcagga tgtaagagag cgcgccaatc aacgcgacgg cggagatata
    10201 aaccagtgca ggtgcgaaac cgtaaccctg cgccaggtac cccaccacca gcggaacggt
    10261 gatgccgccc agaccaccgg cgaagttaaa cacgccgccg gttaaaccaa tcaggcgcat
    10321 cggtgccaga gaagagacca gcgaccaggt aatcgaagca aaaccgttgc cgaagaatgc
    10381 cagcgccatc aggcacataa tcatcatcgg atcgttggtg tagttagcgc ccataatgca
    10441 ggtggagatc agcaagccgc agataatcgg cgttttacgc gcaaagccca gtgaaaagcc
    10501 cttacgtacc agcagatccg cgacccagcc agagagcagg acgccgacaa acgccgcgag
    10561 gaatggcacc gtggtcataa agcctgcttt cagcgccgtg attccttttt cctgggttaa
    10621 atagttcggg aaccaggtta agaaaaacca cagtgtagaa gccaccgcaa attgcccaag
    10681 ataaacgccg atcagtttac gatggaacac cagtttccag tctttggctg ttaacggctg
    10741 acgcgcctct ttcttcaccg gcgcatcacc atccaccaga ccgccgccat cacgaatgta
    10801 atccagttca gctttgctga tacctttggt caggcgcggc ggctgataaa ccttaaacca
    10861 aatcagcgac cagataatgc cgataccacc agtgacaatg aacacccagt gccagctcaa
    10921 catctcctga atccagatca gcagcggagt caaaaacgcc agaccgacaa actgaccaga
    10981 cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg aaccagctgg tcaccatccg
    11041 gttattggtc gggaaagcgg gcgcttcgaa aataccggtt atcgcacgca gaccaattaa
    11101 tgacattaat cccgtggcaa agccctggaa taaagtcgcc actgaccaac caaatatcgc
    11161 aataaaataa gtcacgcgag aacctacgcg atctaaaaac caaccgccgg ggatctggca
    11221 tagcgtataa agccaggcga aggccgaaaa tacatagccc atttccgctt tcgtaatgcc
    11281 gaactcttcc tgaatatggg cggaagccac ggccaggtta gcgcggtcaa cataacaaat
    11341 gaccaccgta ataaagatca tcaccagcgt cagataacgc cgacgccccg gctttgctgc
    11401 attaacggga atatccatag cgagctttct ccagattttg ggcatagcga agccgctcac
    11461 catgccctgt aatttacaga gggttatatt tttgtattgc tgttttagtg cccgatgagg
    11521 ggcttacgtg gcaggaatta ccactctgct acgctgttat cttcatgacg ccagagcgga
    11581 ttacgccagt ccggggcatt tttactgaac tcaataactt tagcttcgtc gatttccaca
    11641 cctaagcccg gtttcgttaa cggtttaaag aagccgccaa ccatactgaa gtcttctttg
    11701 tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat gaatgcccat actttgttcc
    11761 tgaagtaccg cgttatagga aacaaagtcg atatgcaggc aagccgccag tgcaatcggt
    11821 ccgagcggac agtgcggcgc aagggtcaca tcataggctt ctgccattcc agcaattttg
    11881 tagcattcgg taataccgcc tgcatgggag agatccggtt gcagaatcga aataccgcct
    11941 gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc gctcacccgc cgccagtgga
    12001 atatgcgttt gtgccgccaa tttcgggtag tattcggctt gttccgccag caccggctct
    12061 tcaataaaca gcgggcgata cggctccagt tctttaatca gcactttcgc cattggcgcg
    12121 ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat tgccaaaagc ttcacgaatt
    12181 tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt tatcaattag ccccagttct
    12241 tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc gcagggtttt aatgccgtcg
    12301 ataacatccg ccggacgatc gccgccgacc caactgtagg ctttaatttt gtcgcgaacc
    12361 aggccgccca tcagttgcca gaccggcgca ttcagcactt tgcctttgat atcccataac
    12421 gcctggtcga tcccggcaat ggcgctcatc aggattggac cgccacgata aaatccggcg
    12481 cgatacatca cttgccataa gtcattgatg cgcgaaggat cctgaccaat caaatagtca
    12541 cccagctcgt gaactgccgc ttccaccgta cgggcgcggc cttcaatcac gggctcgccc
    12601 caaccgacca cgccttcatc ggtttcaatt ttcaggaaca tccagcgggg aggtaaacga
    12661 tacgtggtaa ttttggtgat tttcattgca ctgcctctcg atacgcctta acaaatgctg
    12721 ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg atagagatcg ctgcctaagc
    12781 ccgccccagc acaacctgcg tctatccact gcgccaggtt ttctggcgtc acgccgccaa
    12841 cggcaaagac tgcgatgtcc gatggcaata ccgcttttaa cgctttgatg tattgcggac
    12901 caaaagccga tgacggaaat attttcagcg cctgcgcgcc cgcttcgagc gcggtaaagg
    12961 cttcggtcgc cgtcgcgcag ccggggcaga cggtcatgcc gtagcccacc gcacggcgga
    13021 tcacttcact atggatattg ggcgtaacga tgagctgaca gcccatcctg gcgagcgcat
    13081 cgacctgttc aggtttcagt accgtacctg cgccaatcaa cgccttgtcg ccatacgcat
    13141 caacgatggc gggaatgctt tgctcccatt gtggggaatt cagcgggatt tcaaccgcgt
    13201 cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc ctcgtcgggc ttaatgccgc
    13261 gcaaaatggc gatcagcgga agtttagttt gccactgcat gagcgatgct ccttatacca
    13321 gcctgaaatg ccgtgtcgcc cgccaccgcc gtcacgtcgc aacccatcgc ctgaaaggct
    13381 tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg tgatggcgtg ttgatgggtc
    13441 acatagtcgc gcatactggc aacctctgcg ccaatcaaca aaccagagag aaattcgctg
    13501 acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc gaacttcaaa aagctgcggc
    13561 aatatggcgg gcgcattaag gccgcgctca aggccagccg cgaaggcatc ggcagagttt
    13621 tcctgcggcg gcaaacctgc gccaatcaat gagtgattta acagtaaatg atgtaattca
    13681 ccggtcatca cggtgcgaaa atcgttgatt tgctggctat cggcctgcac ccatttgcaa
    13741 tgcgtaccgg gcataacata aagagaggaa ggagccagag ctcgcgcgcc gatcaattgt
    13801 gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag agacgcataa tccgggaata
    13861 atccagatat tgtcgccaac agacgttaat tgttcgccaa tagacgaaaa acgggcagga
    13921 acagataaat acggagcaac tttccagccg acattgctgc cgaccattcc tgccatcacc
    13981 actggcgtat tttcttcacg ccagtcggtc gtgacttctg ctaacaccgc agccggagat
    14041 tttccgttca ggcgcgtgac gcctgcttct gattgcctgc tctccaggca gtggtcgccc
    14101 tgataaagcc aggcgcgcag attggtcgat ccccagtcaa ttgcgatgta gcgagctgtc
    14161 atgtgatttc ctttaacctt cgtgtcgagc tggcgatcat ggtaagcgcc gcctgctctg
    14221 ccgcatcgcc gtcctgatgc cgtattgcat cgaacagcgc cttatgttcc tggagcgttt
    14281 gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa aaccgcccgc tgcaacgaac
    14341 tgatcgcaat gctaagttgc tgtaacaccg ggttatgtac cgactgcagc accgcctcgt
    14401 ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg attgttggca atcatctcgt
    14461 tcagcgccga ttcaatctgc gccagatcgc tggaagtcgc gcgctctgct gcccaacggg
    14521 caatcgccgg ttccaccaga tttcgcactt cgctcatggc actgataagc cgtgggtcgt
    14581 agtcgttttc caacacccat tgcagtacgt cagtgtcgag gtaattccac tggttacgcg
    14641 gtgccacaaa cgccccgcga taacgtttca tttcaatcag ccgcttcgcc atcagcgaac
    14701 ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc ttcacagagc tccgcctcag
    14761 ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat ctgtttaccc agcgtaatga
    14821 caatgcgatc ggttttattg agagtcatgg agagtccttg tgctctgtat gttcttctct
    14881 actttacccc gatcgatgca taacgcggca actttgtagt accagcgtga tgacgttcgc
    14941 gtttgccgtg tgcgtaatgt agtacaaact tatattgttg tactacaatt tagatcacaa
    15001 aaagaacaat gcataaaaaa atgacatgcg tcgggcagaa atctgaaaag ggatatcagg
    15061 cgctaaacag gagggaaaga agagtatgct ttcaacggct tagctactcg tttaaaggat
    15121 taatcatgaa gttgaatttt aagggatttt ttaaggctgc cggtttattc ccactggcgc
    15181 tgatgctttc aggctgtatc tcgtatgctc tggtttccca taccgcaaag ggtagttcag
    15241 gaaagtatca atcgcagtca gacaccatca ctgggctatc gcaggcaaaa gatagtaatg
    15301 gaacaaaagg ctatgttttt gtaggggaat cgctggatta ccttatcact gatggtgccg
    15361 atgacatcgt taagatgctc aatgatccag cacttaaccg gcacaatatt caggttgccg
    15421 atgacgcaag atttgtttta aatgcgggga aaaagaaatt taccggcaca atatcgcttt
    15481 actactactg gaataacgaa gaagaaaagg cactggcaac gcattatggt tttgcctgtg
    15541 gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg cacaatccat gagaaaaata
    15601 aaaacatgga ttactcaaag gtgatggcgt tctaccatcc atttaaagtg cgattttatg
    15661 aatactattc acccagaggc attccggatg gtgtttccgc agcattactg ccagtgactg
    15721 ttacgctgga catcattact gcaccgctgc aatttctggt tgtatatgca gtaaaccaat
    15781 aatcagtaag cgggcaaacg cgtttatgct gtttgcccgt ccactgatta attcagcaca
    15841 tacttctcaa tagcaaacgc cacgccatct tcaaggttgg atttggtgac aaagttcgcc
    15901 acttctttca ccgaaggaat agcgttatcc atcgccacac cgacgcctgc atattcaatc
    15961 attgcgatgt cgttttcctg atcgccaatc gccatgattt cttccggttt aatacctaac
    16021 acgtcggcca gtgatttcac acccgtaccc ttgttaacgc gtttatcgag gatttcgagg
    16081 aagtacggcg cacttttcag cacggtatat ttctctttca cttcctgcgg aatacgcgca
    16141 atagcctggt cgaggatggc gggttcatca atcatcatca ctttcaggaa ctgggtattg
    16201 gggtccattt tctccgcttc gcagaacacc agcgaaatgg tggcaacgaa ggattcatgc
    16261 accgtgtagt agctgatatc gcgatttgct gtatacagcg tggtgcggtc cagggcgtgg
    16321 aaatgagaac cgacttcgcg agagagtttt tccaggaaac ggtagtcgtc atagctgaga
    16381 gcggtttgcg ccacggtgct accatcggcg gccttctgta ccaacgcgcc gttataagta
    16441 atgcagtagt cgcccggctg ttccatatgc agctctttca ggtagttgtg aacgcctgca
    16501 taagggcgac ccgtcgttag cacgacattc acgccacggg cgcgagctgc ggcaatcgca
    16561 tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc catatcgata
    16621 gcaatgagtt tgatagccat gagttcccca ggtagattgg ttcctgaccc atgctaacgc
    16681 gattccgctc aaaaatcagt acaacacccg agggaaaagg gggatgcaac gcgggttgca
    16741 tgctccccat ttgcttagcg gaagagtttc cctttcagca gttccatacc tgcggaaagc
    16801 agatcgttat tggcttgtgg tgacacttca ccttgcggtg agagcgcatc aataatcttc
    16861 ggcaattgct ctgccagtaa actggaagct gtactggtat ccacgccaag tttttgcccg
    16921 agatcggaca ccgcatttgt gccgagcgcc gattccagtt gctcgccact aaccgattga
    16981 ttgccctgtt gattactcag ccaggttgag agaatggccc ctaagccgcc actttgcagt
    17041 ttttccagca gcacctgaat gccgccctgc tcctcaaccc aacttaaaat agcctgatat
    17101 ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc catgataatc
    17161 acctgtaaag cgttacgtgt tgacccaaaa agtatagatt tgcgggatga taattgcgga
    17221 ttgcagaaat aaaaagggcg gagatgatct ccgccctttt cttatagctt cttgccggat
    17281 gcggcgtgaa cgccttatcc ggcctacaaa atcatgaaaa ttcaatacat tgcaagattt
    17341 tcgtaggcct gataagcgta gcgcatcagg cacgctcgca tggttagcgc cattaaatat
    17401 cgatattcgc cgctttcagg gcgttctctt caataaacgc acggcgcggt tcaacggcgt
    17461 cgcccatcag cgtggtgaac aactggtcgg cagcaatcgc atctttaacg gtaacgcgca
    17521 gcatacgacg gctttccggg tccatggtgg tttcccacag ctgttccggg ttcatctcgc
    17581 ccagaccttt ataacgctgg atggagaggc cgcgacggga ctctttcacc agccagtcca
    17641 gcgcctgctc gaagctggct accggctgac gacgctcgcc acgttcgata aacgcatctt
    17701 cttccagcaa gccacgcagt ttctcaccca gcgtgcagat acgacgatat tcgccaccgg
    17761 tgataaactc gtgatccagc ggatagtcag tatccacacc gtgggtacgc acgcgaacaa
    17821 tcggttcaaa caggttttgt tcggcattgg tgcggacatc aaacttccac tggctgccgt
    17881 gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta acggtctgct
    17941 catcagagag gtcggcttcc gtcagcgtcg gctgatagat aagctctttc agcattgctt
    18001 tcggataacg acgctccatg cggttgatca ttttctgcgt cgcgttgtac tcagacacca
    18061 atttctctaa cgcttcgcca gccaatgccg gtgcgctggc gttggtgtgc agcgttgcgc
    18121 catccagcgc gatagagatc tggtactgat ccatcgcttc gtcgtcttta atgtactgtt
    18181 cctgcttgcc tttcttcact ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt
    18241 caacgatttc cggcatctga cgatagaaga aggtcaacag cagcgtacga atgtgcgagc
    18301 cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata acgcagtttg tccgggttgt
    18361 actcgtcacg accgatacca cagccaagcg cggtgataag cgtcgccact tcctgagaag
    18421 agagcatctt atcgaagcgc gctttctcga cgttgaggat tttacccttc agcggcagaa
    18481 tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc gcctgcggag tccccttcca
    18541 ccaggtacag ttcggaaagc gccggatcgc gttcctggca gtctgccagt ttgcccggca
    18601 ggcccgctaa gtcgagcgca cctttacggc gggtcatttc acgcgcacga cgcgcagctt
    18661 cacgggcacg ggcagcatcg atgattttgc caaccacgat tttcgcgtcg gttgggtttt
    18721 ccagcaggta ttctgccagc agttcgttca tctgctgttc aaccgctgat ttcacctcag
    18781 aagaaaccag tttgtctttg gtctgggagg agaatttcgg gtccggcact ttcacggaaa
    18841 cgaccgcaat caggccttca cgcgcatcgt caccggtggc gctaactttg gcttttttgc
    18901 tgtagccttc tttgtccatg taggcgttca gggtacgggt catcgccgca cggaagcctg
    18961 ccaggtgagt accgccgtca cgctgcggaa tgttgttggt aaagcagtag atgttttcct
    19021 ggaagccatc gttccactgc aacgccactt cgacgccaat accgtctttt tcagtggaga
    19081 agtagaagat attcgggtgg atcggcgttt tgttcttgtt cagatattca acgaacgcct
    19141 tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc gcgcttgtcg cgcagacgaa
    19201 tggaaacgcc ggagttgagg aacgacaact cacgcagacg tttcgccaga atttcatatt
    19261 cgaactcggt cacattggtg aaggtttcga ggctgggcca gaaacgcacc atggtgccgg
    19321 ttttttcagt ctcgccggta accgccagcg gggcctgcgg tacaccgtgt tcgtagatct
    19381 gacggtgaat tttaccctcg cgctggataa ccagctccag tttttgcgac agggcgttta
    19441 ctaccgaaac accaacgccg tgcagaccgc cggacacttt ataggagtta tcgtcaaatt
    19501 taccgcctgc gtgcagaacg gtcatgatca cttccgccgc cgatacgccc tcttccgggt
    19561 gaataccggt cggaatgccg cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa
    19621 tggtgacgat aatttcttta cagtgacccg cgagcgcttc gtcgatagcg ttatctacca
    19681 cctcgaatac catgtggtgc agaccggtgc cgtcatccgt gtcgccgata tacatacccg
    19741 ggcgcttacg caccgcatcc agccctttca ggactttgat actggaggag tcataagaat
    19801 tcgacatcaa cgtttctcgc tcatttatac ttgggttaat ccgttatttt acccttttcc
    19861 acggtaaaca tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc actgatcgcg
    19921 ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg caagcagccc gcgacgctca
    19981 tcatcaagct cagaggcaaa atcatctatc aggtagagac accgccgccc gctttcacgg
    20041 gtgaggaact ctccttgcgc cagacgtaag gcgcacatca acagcttaag ctgcccacgc
    20101 gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg
    20161 ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat tacgttccag cacctcagca
    20221 tattctgtct ctttctccca gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga
    20281 aattgcttac aggtatcgac catatcggcc gcgataccgg cgctatactc cgcgcgccag
    20341 gtgctgattt gctccgccag cgggatcagt tctttatccc acgggcgtag ctgttcgtaa
    20401 cgtgtcacct ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag attgctccag
    20461 gcggtgaaaa atccgggttc gttgtgaaag catccccagt cgaggaatgc tcttctgtat
    20521 ttggggccgc cgttgagtaa agtaaaccct tctggcgtta tcaactgcat tggcatcagg
    20581 tgcgccagtt ccgcgacttt atgcccgtct gtaccgtcga tgcggacttt gctgtcgccc
    20641 tgtttgtctt tggttaagcc aatcgctgtc tcgcgctctt cgccctgtaa tcgcccgtgg
    20701 agaacaaacg cctcctgctc atggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc
    20761 cgaccatggc cgagcgtata gatggcttcc agcacgctgg ttttgccact gccgttggca
    20821 cctaccagaa agttaaagcc gggagataaa gcgagatccg cggtttcaat gttgcggaaa
    20881 tcgcggatca acaagcgggt gagggacatt acagtctcat tggcatgaca acataggcag
    20941 ccgactgtga tgcggcatct tcaatctgca cgctggaaac cgaatcggtc agcatcatgc
    21001 ggacgttttc gcatttcagc gcgttcagaa catccagcac atagctgacg ttgaagccga
    21061 tttccatctc cgcaccgcta taggtaacgt cgaggatctc ttccgcttct tcctgttccg
    21121 ggttgttggc ggtgattttc agctggtttt cgctgacata aagacgtacg ccgcggaatt
    21181 tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt gagcagatcg cagccagctt
    21241 ccagatgttt gtccgggttc ttcggcagaa cgcggcgata atccgggaag cgaccatcca
    21301 ccagtttgga ggtgaagata aagtcgccaa cgtgggcgcg aatgttgttg ctgccaatct
    21361 gtacgcgcag cggattgtcg ccgccgtcga gcatacgcat cagttcaatc acgcctttac
    21421 gcggtacgat caccgaatgg cttggcaaag attgaccaat tggcattgaa cagaccgcca
    21481 gacggtggcc gtcggttgcc acggtgcgca gttcttcacc ttcggtttca aacagcatac
    21541 catttaagta atagcgaacg tcctgatgcg ccatagaaaa ctgggtcgct tcaatcagac
    21601 gcttcatcgt tgcctgcggc agggtaaatt cgacttcact ctgccagtca tcgaggttcg
    21661 ggaaatccgc cgctggcagg gtagacagcg aaaaacggct acgcccggag cgtaccagca
    21721 tccgttcacc ttccagctgc acggcaattt ccgcgccttc aggcagacca cggcagatat
    21781 caaagaattt gcgcgccgga acggtcgtcg caccaggttc gtgcggctga accagcgcaa
    21841 cgcgtgccac catttccatc tcgagatcag taccggtcag cgacaacgta ccgtcagcaa
    21901 cctgtaacag cagattaccg agaatcggta gcgtaggacg accacctaac ggaccgctca
    21961 cctgttgtag cggttttaat aaatgctcac gttctacggt aaatttcata ggtttacgat
    22021 gacaatgttc tgattaaatt tgaaaaatct tctttgatat cgtggctctc ttcacgcaac
    22081 tgctcgatct tccggcaggc atgaagcacc gtcgtgtggt cacgaccacc aaacgcatcg
    22141 ccaatctccg gcagactgtg gttagtcagc tctttcgcca gcgccatcgc catctggcgc
    22201 ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag
    22261 tactccgcca ccgtcttctg aatattgtcg atagtgacca gtttttcctg caatgccagc
    22321 aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt aaagttggca
    22381 ttggcaatga cgcggttcag cgccccttcc agctcacgta cgttagatcg tagacgcttg
    22441 gcgataaaga acgccacttc gcccggcaaa cgaatgtcgt tttcgtcggc ctttttcatc
    22501 aggatcgcca cacgggtttc cagctctggc ggttcgattg ccacagtcag tccccaaccg
    22561 aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg atccgaggtg
    22621 agaatgatct gttgattacc ttccagcagg gcgttgaagg tatggaaaaa ctcttcctga
    22681 gatcgttctt tattagcaaa aaactgaata tcgtcgatca gcagtgcatc tacggaacgg
    22741 tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca
    22801 aagcgctcgg agtgcatata aaccactttg gcattcggct tgcgcgccat aatgccgtta
    22861 cccaccgcat gcagcaggtg agttttaccc agacccgtgc cgccataaag gaacaacggg
    22921 ttataggcac cgccagggtt atccgccacc tggcgagccg ccgcgcgcgc cagttggtta
    22981 gatttacctt caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga acgataggtt
    23041 ggttctgccg gggccgggac gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc
    23101 ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc
    23161 gtttgcgtca ccggtttggt gccgacttca aaacgcagct gtggggcatc cgctccgcag
    23221 aaactggtta gcagtccatt gatattatta aggtacttgt cccgtaccca atcgaggaca
    23281 aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca gttccgcctg caatgggcgt
    23341 atccacatac tgaattctgt ggctggtaac tcatcctgca atcgggcaag acactgctgc
    23401 caaagcgaaa gtgacacggc ggactccact cgaacaaaag tcgataatga ctaaggctga
    23461 aacattcatg attgttgacg tacgtcgaaa agaccctgct tgagggtgac gcacgaaccg
    23521 ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg cggatcatag
    23581 cctaaactgc gcaagagatc ttctgtttct cacagatttt cccgatttat ccacaggact
    23641 ttccagaact cgctaagtgt aaacgatcct gccgcgaggc gggcacgatt tacgccgcat
    23701 attggaaaat ttaatgacca tagacaaaaa ttggcttaat cgatctaata aagatccagg
    23761 acgatccttg cgctttaccc atcagcccgt ataatcctcc acccggcgcg ccatgctggt
    23821 ttccactggt gtgaggtcgt acattttccc tgcgaaaagg tgcggaaaag cgcggtaaat
    23881 aaggaaagag aattgactcc ggagtgtaca attattacaa tccggcctct ttaatcaccc
    23941 atggcttcgg tgtccatcgt ttcatttttc ggcggatatc caataaagcc attgaattta
    24001 ttcaagttta ggtagaaatc gccatgaaac gcacttttca accgtctgta ctgaagcgca
    24061 accgttctca cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac
    24121 gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa gtaataaagc taacccctga
    24181 gtggttaagc tcgcatttcc cagggagtta cgcttgttaa ctcccagtca attcacattc
    24241 gtcttccagc agccacaacg ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat
    24301 tcgctggggc atccccgtat cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat
    24361 gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc
    24421 ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct
    24481 ctctcggaag cgttggaaaa attatggcgc cgccactgtc gcctggctcg cgggtcctga
    24541 tagccctcat tcgggtctat caacgcctga ttagtccgct actcgggccg cattgtcgtt
    24601 tcactccaac ctgttcaagc tacggaattg aggcattgcg caggtttgga gtgataaaag
    24661 gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc tttacaccct ggtggtgacg
    24721 atcccgtccc gcccggacca tttgatacca gagaacacta acgatggatt cgcaacgcaa
    24781 tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct gggagcagga
    24841 taaaaacccg caacctcagg cccaacagac cacgcagaca acgaccaccg cagcgggtag
    24901 cgccgccgac cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga
    24961 cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt gagcaagctc tgctgccggc
    25021 ttacccgaaa gagctgaact ctacccagcc gttccagctg ctggaaactt caccgcagtt
    25081 tatttatcag gcacagagcg gtctgaccgg tcgtgatggc ccggataacc cggctaacgg
    25141 cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg ctggctgaag gtcaaaacga
    25201 actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac acgtttacca aaacgtttgt
    25261 cctgaaacgt ggtgattacg ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa
    25321 accgctggaa atctccacct ttggtcagtt gaagcaatcc atcactctgc caccgcatct
    25381 cgataccgga agcagcaact tcgcactgca caccttccgt ggcgcggcgt actccacgcc
    25441 tgacgagaag tatgagaaat acaagttcga taccattgcc gataacgaaa acctgaacat
    25501 ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg cgtggatccc
    25561 gcataacgac ggtaccaaca acttctatac cgctaatctg ggtaacggca tcgccgctat
    25621 cggctataaa tctcagccgg tactggttca gcctggtcag actggcgcga tgaacagcac
    25681 cctgtgggtt ggcccggaaa tccaggacaa aatggcagct gttgctccgc acctggatct
    25741 gaccgttgat tacggttggt tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg
    25801 gatccatagc tttgtgggta actggggctt ctccattatc atcatcacct ttatcgttcg
    25861 tggcatcatg tacccgctga ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct
    25921 gcagccgaag attcaggcaa tgcgtgagcg tctgggtgat gacaaacagc gtatcagcca
    25981 ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg ctgggcggct gcttcccgct
    26041 gctgatccag atgccaatct tcctggcgtt gtactacatg ctgatgggtt ccgttgaact
    26101 gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc cgtactacat
    26161 cctgccgatc ctgatgggcg taacgatgtt cttcattcag aagatgtcgc cgaccacagt
    26221 gaccgacccg atgcagcaga agatcatgac ctttatgccg gtcatcttca ccgtgttctt
    26281 cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc agcaacctgg taaccattat
    26341 tcagcagcag ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa
    26401 aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc gccttttttc
    26461 ttttcgtagg gcggataagc accgcgcatc cgccacacaa agcaacagga acatcatgag
    26521 cgataatgac actatcgtag cccaggccac gcctccggga cgtggcggcg ttggcatcct
    26581 gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta aactacctaa
    26641 gccgcgctac gccgattatc ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg
    26701 gattgcgcta tggttccctg gcccgaactc gttcaccggc gaagatgtgc tggaactgca
    26761 aggtcatggc ggtccggtga tcctcgacct gctgttaaaa cgcattctga ccattcccgg
    26821 cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg tttcttaacg ataaactcga
    26881 cttagcccag gccgaggcga ttgccgatct tatcgacgcc agttcggaac aggcggcccg
    26941 ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg gtcaaccatc tggtggaagc
    27001 cctcacccac ttgcgcattt acgtcgaagc ggcaattgat ttccctgatg aagagatcga
    27061 tttcctctcc gacggcaaaa ttgaagccca gcttaatgac gtgattgccg atctcgatgc
    27121 agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa gggatgaagg tggtgattgc
    27181 cggacgtcct aacgccggta aatcgagcct gttaaacgcg ctggcgggcc gtgaagcagc
    27241 aatcgtaact gatatcgccg gaaccacacg tgacgtgctg cgtgagcata tccacattga
    27301 cggaatgccg ctgcatatca tcgataccgc tgggctacgt gaagccagtg acgaagtgga
    27361 acgtattggt atcgagcgcg cgtggcagga aattgaacag gccgaccgcg tgctgtttat
    27421 ggtcgatggc accacaacag acgccgtgga tccggcagag atctggccgg aatttatcgc
    27481 ccgtctgcca gcgaaactgc cgatcaccgt ggtgcgcaat aaagccgata tcaccggcga
    27541 aacgctggga atgagtgaag tgaacggtca cgcgttaatt cgtctctcgg caaggacagg
    27601 tgaaggcgtg gaggtgctgc gtaaccatct caaacagagc atgggctttg acaccaacat
    27661 ggaaggcggc ttcctggcgc gtcgtcgcca cctacaggcg ctggaacagg cagcggagca
    27721 tttgcagcaa ggtaaagcgc aactgttagg tgcctgggcg ggtgaactgc tggcggaaga
    27781 gttgcgcctg gcacagcaga acttaagcga aatcaccggg gaatttactt cagacgacct
    27841 gctggggcgg attttctcca gcttctgtat tggtaagtaa ccgcgcttac gaagccgtat
    27901 tctgactgtc agatgcggct tcgcttcatt gttagcactc ctgttattcc tcaacccttt
    27961 ttttaaacat taaaattctt acgtaattta taatcttaaa aaaaagcat
//
LOCUS       DAFAVF010000049        27857 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFAVF010000049 DAFAVF010000000
VERSION     DAFAVF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.049"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(31..615)
                     /locus_tag="LGK42_002054"
     CDS             complement(31..615)
                     /locus_tag="LGK42_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2658304.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            complement(985..1464)
                     /gene="folK"
                     /locus_tag="LGK42_002055"
     CDS             complement(985..1464)
                     /gene="folK"
                     /locus_tag="LGK42_002055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBK2658305.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(1461..2879)
                     /gene="pcnB"
                     /locus_tag="LGK42_002056"
     CDS             complement(1461..2879)
                     /gene="pcnB"
                     /locus_tag="LGK42_002056"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBK2658306.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2918..3844)
                     /gene="gluQRS"
                     /locus_tag="LGK42_002057"
     CDS             complement(2918..3844)
                     /gene="gluQRS"
                     /locus_tag="LGK42_002057"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBK2658307.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(3881..4336)
                     /gene="dksA"
                     /locus_tag="LGK42_002058"
     CDS             complement(3881..4336)
                     /gene="dksA"
                     /locus_tag="LGK42_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBK2658308.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(4514..5218)
                     /gene="sfsA"
                     /locus_tag="LGK42_002059"
     CDS             complement(4514..5218)
                     /gene="sfsA"
                     /locus_tag="LGK42_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK2658309.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(5233..5763)
                     /gene="thpR"
                     /locus_tag="LGK42_002060"
     CDS             complement(5233..5763)
                     /gene="thpR"
                     /locus_tag="LGK42_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBK2658310.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5837..8266
                     /gene="hrpB"
                     /locus_tag="LGK42_002061"
     CDS             5837..8266
                     /gene="hrpB"
                     /locus_tag="LGK42_002061"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBK2658311.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            8462..10996
                     /gene="mrcB"
                     /locus_tag="LGK42_002062"
     CDS             8462..10996
                     /gene="mrcB"
                     /locus_tag="LGK42_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBK2658312.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            11216..13459
                     /gene="fhuA"
                     /locus_tag="LGK42_002063"
     CDS             11216..13459
                     /gene="fhuA"
                     /locus_tag="LGK42_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBK2658313.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            13510..14307
                     /gene="fhuC"
                     /locus_tag="LGK42_002064"
     CDS             13510..14307
                     /gene="fhuC"
                     /locus_tag="LGK42_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBK2658314.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            14307..15197
                     /gene="fhuD"
                     /locus_tag="LGK42_002065"
     CDS             14307..15197
                     /gene="fhuD"
                     /locus_tag="LGK42_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBK2658315.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            15194..17176
                     /gene="fhuB"
                     /locus_tag="LGK42_002066"
     CDS             15194..17176
                     /gene="fhuB"
                     /locus_tag="LGK42_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBK2658316.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(17211..18491)
                     /gene="hemL"
                     /locus_tag="LGK42_002067"
     CDS             complement(17211..18491)
                     /gene="hemL"
                     /locus_tag="LGK42_002067"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBK2658317.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            18716..20137
                     /gene="clcA"
                     /locus_tag="LGK42_002068"
     CDS             18716..20137
                     /gene="clcA"
                     /locus_tag="LGK42_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBK2658318.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            20161..20226
                     /locus_tag="LGK42_002069"
     CDS             20161..20226
                     /locus_tag="LGK42_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658319.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            20219..20563
                     /gene="erpA"
                     /locus_tag="LGK42_002070"
     CDS             20219..20563
                     /gene="erpA"
                     /locus_tag="LGK42_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBK2658320.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(20610..21233)
                     /locus_tag="LGK42_002071"
     CDS             complement(20610..21233)
                     /locus_tag="LGK42_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBK2658321.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(21271..22071)
                     /gene="btuF"
                     /locus_tag="LGK42_002072"
     CDS             complement(21271..22071)
                     /gene="btuF"
                     /locus_tag="LGK42_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBK2658322.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(22064..22762)
                     /gene="mtnN"
                     /locus_tag="LGK42_002073"
     CDS             complement(22064..22762)
                     /gene="mtnN"
                     /locus_tag="LGK42_002073"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK2658323.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22846..24363
                     /gene="dgt"
                     /locus_tag="LGK42_002074"
     CDS             22846..24363
                     /gene="dgt"
                     /locus_tag="LGK42_002074"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBK2658324.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            24493..25917
                     /gene="degP"
                     /locus_tag="LGK42_002075"
     CDS             24493..25917
                     /gene="degP"
                     /locus_tag="LGK42_002075"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBK2658325.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            26072..27229
                     /gene="cdaR"
                     /locus_tag="LGK42_002076"
     CDS             26072..27229
                     /gene="cdaR"
                     /locus_tag="LGK42_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBK2658326.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDGER"
     gene            complement(27318..27704)
                     /locus_tag="LGK42_002077"
     CDS             complement(27318..27704)
                     /locus_tag="LGK42_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBK2658327.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
BASE COUNT         6414 a         6977 c         7715 g         6751 t
ORIGIN      
        1 attcattcaa tcaatttaat tggtgaatac ttactgataa gtaatggtgt atgcagtgtt
       61 agtttttaca taaccagcag ttgctgtttg gtctgcgacg gcacgaacgt aagacgcttt
      121 aaaatcataa acagcagatt ttgtcgttgc atccagggct ttagtataca catcgccagg
      181 gttgttaatt tgaacctgtt tgatagtgga accatcaata ttgtgcagag cgatattcac
      241 gccgtcagac gggttgttaa tagacatgtc gttgttcagc gttcctttgc tattaccaaa
      301 gaaaacagaa ccgaaggtca ttttagctgt actacctgga ttcggatttg ctttgctgca
      361 gtcaacggtg atgctgaaag gtttggcttt agcgccaacg gtgtcattca gcacgccagc
      421 atcgatttca ccaacagttg cagtttgcag caggatcaga ccatctttat taccgccgtc
      481 aacgcgggtt tcacaggtgt tatcaacaac cagaccactg atatttaact gaccaccatc
      541 catatcagca gaagcagtac ctgcaaccat tgccagcata agaccagaca gggcaaaacc
      601 taatttttta gacataaaaa tccttgaata aaattccatt agtcagacta ctggtttgaa
      661 gaatgactat tcatgacaca aatgttataa gcagatgcta gatattaatg agcaatgata
      721 tttgttacca aaatttacaa ccattgttca ttaggacgcc tattgtgcac tttaagggct
      781 tttgaacaaa ttaaattttc ttaattcaaa attaagcaaa aataaattca caagtgcaat
      841 tggttagggt atggcgatgg gattgatatt tatctgcggg aatttatatt atgtttctgc
      901 aaaaatgttt aaactaaaat ttttattaag gaaagagagg atgtatgatt ttcagaaaat
      961 atttatattc gtaatataaa taaattacca tttgcttaat ttgtcaaatg ctcttgtatg
     1021 taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa tctcaaacag
     1081 cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca ggcgttcggt
     1141 atttatcact tcattaccaa acagcatgat atcgagatcc agcgtgcgtg gtccccagcg
     1201 ttcagcttta cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt tgagtaactc
     1261 ttccggggcg agcgaagttt caagcgccac ggcggcgttg aggtaatcgg gttgatcttg
     1321 cggccccagc ggtggggtgc ggtaaaacga agaaacggca agaatgtggc tttcagggat
     1381 atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc
     1441 tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga
     1501 cgacgagtac gacgacgcgg tgacggttct tcatccagct cgttaagcat ccctttttga
     1561 tctggtggtg cggaaacctg gaactcaccc caccatttca ccagacgctg cagttcagca
     1621 ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa cttaggatgc
     1681 tccagcagtt tccatgcgcg tttaccctga cgacgggaca tacgcaactg caactgccag
     1741 atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca ggcttcgtcc
     1801 agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggca
     1861 atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag gaacgccggg
     1921 ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc
     1981 cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc
     2041 tggaacagat gatattcaca caacagctta taggtatcgt aaccgtagcc cgcttgtagc
     2101 agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcaacag ggtagcgagg
     2161 cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt
     2221 accgcgcgca gcatacgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga
     2281 cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac ggtaaaatcc
     2341 gctacgctgt aatagaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg
     2401 gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg
     2461 tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca
     2521 aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg
     2581 cgcacctgct caggcgtggc gttagtggtt acgtcaaaat ctttcggctt tttgccgagt
     2641 aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt attgagcctg
     2701 tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc
     2761 gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt
     2821 agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa
     2881 catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg
     2941 ttgaatttac aattgccgac tcgggcacgg cggtaagccg ccagtttttg acggctgact
     3001 gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt
     3061 gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc gcatgattct
     3121 gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg taatctggca
     3181 ctttccagcc aaaaagctgg tacagcgaga tttgccttac tgttggttca atcagatcag
     3241 ccccacgcac tatttctgta acgccctgga aatgatcatc aaccacaaca gccaggttgt
     3301 aggcgaacaa cccatcacgg cgatgaatga taaaatcttc ccgtgccagt ttttcgtcgg
     3361 cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc tggcggatac
     3421 gcactgcggc attgtctggt ccatgatgca acacccggca atgaccgtcg taaataccgc
     3481 caatgctttg aatacgcgca cgcgtacagg tgcagtaata acttagtcct tgttcatgta
     3541 accaggcgag tgcttcacga taggcgtggt gacgttgcga ttgccagaga acatcgccgt
     3601 cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt
     3661 cacgaggcgg gtcgatatct tctatgcgta ccagccagcg accttgccgg gcgcgagcct
     3721 gcaaatagct gccgagcgcg gcgatcagag agccaaaatg aagctcgccg gaaggagagg
     3781 gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga
     3841 gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt
     3901 cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca
     3961 gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca
     4021 cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca
     4081 gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg
     4141 cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg
     4201 cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct
     4261 tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt
     4321 tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg
     4381 aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac
     4441 acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca
     4501 gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg ccttcagcag
     4561 aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt
     4621 gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca
     4681 cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt
     4741 gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg
     4801 taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa
     4861 tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt
     4921 ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtgttga
     4981 cgcaaataaa tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt
     5041 tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcattg
     5101 cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta
     5161 aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagag aattccattg
     5221 cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc
     5281 acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcataagacc agttaaaacc
     5341 tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa
     5401 cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca tattcgccag
     5461 ctggattaag ccgcgtggag gctgacgcat ccctaaccac accacacgcg aacgcagcca
     5521 ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag
     5581 agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag tcagatgcaa
     5641 attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat
     5701 aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt gcggttcaga
     5761 catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa
     5821 ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttatctg aattacttac
     5881 cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg ggaaatcaac
     5941 ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctgctgga
     6001 gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacgactg gcggagctgc ttaacgaaaa
     6061 gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg
     6121 cctggaagtg gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc cggaactgag
     6181 cggtgttgga ttggtgatcc tcgatgaatt tcatgagcgc agcttgcagg cggatttggc
     6241 gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat
     6301 gtcggctacg ctggataacg accgcttgca gcaaatgctg ccagaagcgc ccgtcgtcat
     6361 ctcagaaggg cgctcgtttc cggttgaacg ccattattta ccgctgcccg cgcatcagcg
     6421 ttttgacgaa gccgttgcgg tagccaccgc cgaaatgctg cgtcaggaaa gcggatcatt
     6481 actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tggcttcgcg
     6541 catcggcagt gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg
     6601 aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc
     6661 tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc aggagcgtgt
     6721 ggcgcgtttt gatccgcgca cggggcttac gcgactgatt actcaacgcg ttagccaggc
     6781 atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatca gcctgcattt
     6841 aatcgccaaa gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga tcttacaaag
     6901 cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat
     6961 gagctggctg gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc tgttacaaat
     7021 gctgggggcg ctggagggtg aacggcttag tgcgcagggg caaaaaatgg cagcgctggg
     7081 taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg aagctgctac
     7141 cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg
     7201 cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg
     7261 cttaaacgta cgtggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggggc
     7321 gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac tggcaaacgg
     7381 catgggagcg atgctcgatg ccaacgacgc gctaagccgc cacgaatggt tgatcgcacc
     7441 gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgt taccggtcga
     7501 tattgatgaa ttagtacaac gctgtccgca gctggtgcag caatctgaca ccgtggaatg
     7561 ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc agttgacggt
     7621 gaaagtgcag ccactggcga aaccgtcaga agacgagttg catcaggcga tgcttaacgg
     7681 cattcgtgaa aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt
     7741 gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga
     7801 aagtttattg gcgacgctgg aaacgtggct gctgccacat atgactggcg tacattcact
     7861 acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg attggggaat
     7921 gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat
     7981 cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aagagatgtt
     8041 tggcgaggcc accaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggagttgct
     8101 ttcacctgcc caaaggccat tacaaatcac acgagatttg agcgccttct ggaaaggagc
     8161 gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga
     8221 cccggcaaat actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc
     8281 ctctccctgt gggagagggc cggggtgagg gcatcagcgc gcacgttcac cctcacccta
     8341 accctctccc tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc
     8401 tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
     8461 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
     8521 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
     8581 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
     8641 tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat
     8701 cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg
     8761 gcaactgcct gcggcagttt atggccgaat ggtcaatctt gagccagaca tgaccatcag
     8821 caagaacgag atggtgaagc tgctggaggc gacccagtat cgtcaggtgt cgaaaatgac
     8881 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgattcgcc gtccgtttga
     8941 tttcccggac agtaaagaag gacaggtgcg cgcgcgtctg acctttgatg gcgatcatct
     9001 ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
     9061 tctgatcacc atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg
     9121 tttcccggat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca
     9181 tgatggaatc agtctctact caatcggacg tgcggtgctg gcaaacctga ccgccggacg
     9241 cacggtacag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
     9301 cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg
     9361 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     9421 cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga
     9481 agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta
     9541 caacccgtgg cgtaacccaa aactggcgct ggagcgacgt aatctggtgc tgcgtctgct
     9601 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgcac gtccgctggg
     9661 tgttcagcca cgcggtgggg tgatctctcc acagccagcc tttatgcaac tggtgcgtca
     9721 ggagctgcag gcgaaattag gcgataaggt aaaagatctc tccggcgtga agatcttcac
     9781 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc
     9841 actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt
     9901 tagtggtgaa gttcgtgcga tggtcggtgg ttctgagccg cagtttgcgg gctacaaccg
     9961 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgactgc
    10021 cttaagccag ccgaaaatct atcgtctgaa tacgtggatt gcggatgcgc caatcgcgct
    10081 gcgtcagccg aatggccagg tctggtcacc gcagaatgat gaccgtcgtt atagcgaaag
    10141 cggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtaaatct
    10201 ggggatggcg ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa
    10261 agatcagttg catccggttc cggcgatgct gctgggggcg ttgaacttaa cgccaatcga
    10321 agtggcgcag gcgttccaga ccatcgccag cggcggtaac cgtgcaccgc tttctgcact
    10381 gcgttcggta attgcggaag atggcaaggt gctgtatcag agcttcccac aggcggaacg
    10441 cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
    10501 cggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
    10561 gactaccaac aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat
    10621 cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat
    10681 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
    10741 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
    10801 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca
    10861 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
    10921 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
    10981 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
    11041 ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca
    11101 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
    11161 ttataataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
    11221 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
    11281 agcggtaagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
    11341 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
    11401 ggcgcgacag tctgctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
    11461 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
    11521 gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca
    11581 cctgatcatt cgcggctttg cggcagaagg ccaaagccag aataactatc tgaatggcct
    11641 gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc
    11701 tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt
    11761 gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg
    11821 tactgacagc ctgttccaga ctggttttga ctttagcgat tcgttggatg atgacggtgt
    11881 ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga
    11941 agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt
    12001 caccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa
    12061 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg
    12121 ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga
    12181 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca
    12241 aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg
    12301 tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga
    12361 tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga
    12421 tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc
    12481 ctggtttggt tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaataccga
    12541 tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataagca
    12601 gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct
    12661 gggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga
    12721 taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg
    12781 tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga
    12841 tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc
    12901 ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct
    12961 gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg
    13021 cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac
    13081 ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt
    13141 gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg
    13201 tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc
    13261 ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt
    13321 cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt
    13381 cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac
    13441 cgcaaccttc cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag
    13501 ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc
    13561 tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg
    13621 aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt
    13681 ggccgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc
    13741 tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca
    13801 gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgttg
    13861 gggcgctttg gggcggcaga tcgcgaaaaa gtcgaggaag ctatctcgct ggttggctta
    13921 aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca acgggcgtgg
    13981 atcgccatgc tggtggcgca ggatagctgt tgtctgttgc tcgacgaacc gacttcggcg
    14041 ctggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggagcgt
    14101 ggcctgacgg tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat
    14161 ctggtcgccc tgcgcggtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc
    14221 ggcgaaactc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt
    14281 gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac
    14341 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
    14401 ctattgaccc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc
    14461 tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac
    14521 caccattgcc ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc
    14581 tgaccgaaat gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa
    14641 tgctggcacg tattgcgccg ggtcgcggat ttaacttcag cgatggcaaa cagccgttgg
    14701 cgatggcgcg taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg
    14761 aaacgcattt agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc
    14821 gtggtgcacg cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg
    14881 gtccaaacag cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag
    14941 gggaaaccaa cttctggggc agtaccgccg tcagtatcga tcgtctggcg gcgtataaag
    15001 acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa
    15061 ccccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacccgcag
    15121 tctggtttta tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg gataacgcca
    15181 tcggaggtaa agcgtgagta aacgaattgc gctttttccg gcgttattgc tggcgctgct
    15241 ggtgattgtc gctacggcgc tgacctggat gaacttttcg caggcgctgc cgcgcagtca
    15301 gtgggcgcag gccgcctggt cgccggatat taacgtcatc gagcagatga tttttcacta
    15361 cagcttgtta ccacgtctgg cgatttctct actggtgggc gcgggcctgg ggctggtggg
    15421 cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc
    15481 tacaggcgcg caactgggga ttaccgtcac cacgctctgg gcgatccccg gtgctatggc
    15541 gagccagttt gccgcgctgg tgggcgcttg tgtcgttggc ttaatcgtct ttggcgtcgc
    15601 gtgggggaaa cggctttcgc cggttacgct tattctcgcg gggctggtgg tgagccttta
    15661 ttgcggggca attaatcagc tactggttat cttccatcac gaccagctgc aaagcatgtt
    15721 cctgtggagc accggaacgc tgacgcaaac cgactggggc ggcgttgagc gtttatggcc
    15781 gcagctgctg ggcggcgtga tgctgacgtt actgctactt cgtccgttaa ccctgatggg
    15841 gcttgatgat ggcgtggcgc gcaatctcgg gctggcctta tcgctcgcgc gtctggctgc
    15901 gctgtcgctg gcgatagtca tcagtgcgct gctggtgaat gcggtgggga ttatcggctt
    15961 tatcgggttg ttcgcgccac tgctggcaaa aatgctgggg gcgcggcgtt tgttgccacg
    16021 gttgatgctg gcgtcgctga ttggtgcgtt aattctgtgg ctttctgatc aaatcatcct
    16081 ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc
    16141 gccgctgcta ctgtggctgc tgccgcgttt acggagcatt agcgcgccgg atatgaaggt
    16201 caacgatcgt gtcgcgactg aacgccaaca tgtgctggcg tttgtcctcg cgggcggcgt
    16261 gctgctgttg atggctgtgg tagtggcgct gtcgtttggt cgtgatgcgc acggctggac
    16321 gtgggcgagc ggggcgttgc ttgaggattt gatgccctgg cgctggccgc gaattatggc
    16381 ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt attattcagc ggctgaccgg
    16441 aaacccgatg gcaagcccgg aagtgctggg gattagctct ggcgcagcgt ttggcgtggt
    16501 gttgatgctg tttctggtgc cgggtaatgc ctttggctgg ctgttacctg caggcagtct
    16561 cggggcggcg gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc
    16621 acaccgcatg ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat
    16681 gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac
    16741 ctacaacgcg accgatgcgc aggtctggcg caccggaatt gtgatggtga ttttgctggc
    16801 gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcccg
    16861 agccgtagga atggcgctaa cgccgacgcg aattgcgctg ctgctgttag cggcttgcct
    16921 gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca
    16981 tattgcgcgg atgatgggct ttcgacggac gatgccgcac atcgtaattt cggcgctggt
    17041 gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca
    17101 gatcccggcg gggctgctgt caacctttat cggcgcgcca tattttatct atttgttgag
    17161 aaagcagagc cgttaattga atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc
    17221 gcaaacaccc gtcgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg
    17281 gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcgtc cagcatcata
    17341 tggaagaaac gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg
    17401 gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga
    17461 attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc
    17521 agcgtttcgt gaacgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc
    17581 gcaattgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca
    17641 tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt
    17701 ccgaggcagg ttaaatctgg cactacgccg taataatcct gtgcaccagc cagtgccacg
    17761 cggaagccag tcattacttc atcgatgatc agcaatgcgc caaattcgtc gcacagcgcg
    17821 cgcagacctg gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc
    17881 tcaacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc
    17941 agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac acccggcgag
    18001 tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga
    18061 ccgtggtaac aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg
    18121 cggatggcgc tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc
    18181 gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag
    18241 cttaaaccac gctcggcggc ttcaatcacg gcattgcgga ttgccggatg gttatggccc
    18301 agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac
    18361 agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg
    18421 cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca
    18481 gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta
    18541 ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt
    18601 tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg
    18661 aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa
    18721 aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat
    18781 tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt
    18841 cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa
    18901 ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc
    18961 ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc
    19021 cgaagcaggg ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt
    19081 tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg
    19141 catggtgcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt
    19201 gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc
    19261 tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga
    19321 agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt
    19381 cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg
    19441 taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt
    19501 tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg
    19561 tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg
    19621 tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat
    19681 cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat
    19741 taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc
    19801 gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata
    19861 tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat
    19921 tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact accagctcat
    19981 tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa
    20041 accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc
    20101 gcgaagcaag gctgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata
    20161 atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat
    20221 gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag
    20281 cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg
    20341 ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac
    20401 catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg
    20461 cggttccgtt gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa
    20521 cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc
    20581 ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc
    20641 ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc
    20701 atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata
    20761 ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg
    20821 gccagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg
    20881 attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc
    20941 agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc
    21001 agcacgatgg tcagcatgct ggtgaccatt gcaacgacca gatcggtggg atctttcacc
    21061 caaaataccg ggccgtgatc cagcgccatg tcgcgaattg ttccgccgcc tactgcggta
    21121 accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg
    21181 ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa
    21241 tccccactga gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag
    21301 ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg
    21361 aataacggga attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg
    21421 tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg
    21481 cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg
    21541 gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac
    21601 acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat ccagcaggga
    21661 ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc
    21721 attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc
    21781 cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt
    21841 cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg
    21901 cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa
    21961 ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag
    22021 tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt
    22081 tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat
    22141 cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg
    22201 ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg
    22261 cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc
    22321 cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg
    22381 cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt
    22441 cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat
    22501 cgcccacttt cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg
    22561 gcttgcagtg ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg
    22621 atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga
    22681 gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg
    22741 caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt
    22801 taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat
    22861 ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa
    22921 catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt
    22981 cgtcgtctgc aacaaaagac gcaggttttt ccattggagc gcaatgccgc cgtgcgcacg
    23041 cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta
    23101 agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct
    23161 tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt
    23221 cattttggcg aagcagcgat aaatgactgg tttcgccaac gtttgcatcc ggaagatgcc
    23281 gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg
    23341 gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg
    23401 aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ctgggcacag
    23461 gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca
    23521 catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg
    23581 ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa
    23641 gctgccgacg acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata
    23701 tttaccgttg agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt
    23761 tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc
    23821 cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta
    23881 ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat
    23941 catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc
    24001 gctgtaaaac atgtgtttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg
    24061 gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt
    24121 actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg cttattccac
    24181 aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat
    24241 tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgttgca ggattatatc
    24301 agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa
    24361 taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg
    24421 gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg
    24481 agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt
    24541 ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag
    24601 cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac
    24661 gtagaaggta gcacaaccgt taatacaccg cgtatgccgc gtaatttcca gcagttcttc
    24721 ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag
    24781 gggggccagg gcggtaatgg cggcggccag caacaaaaat tcatggcgct gggttccggc
    24841 gtcatcattg atgccgataa aggctatgtc gtcaccaaca accatgttgt tgataacgcg
    24901 acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa
    24961 gatccgcgct ctgatatcgc gctgatccaa atccagaacc cgaaaaacct gaccgcaatt
    25021 aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg
    25081 tttggtctgg gagagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg
    25141 aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc
    25201 ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca
    25261 ccggacggcg gcaacatcgg tatcggtttt gctatcccga gcaacatggt gaaaaacctg
    25321 acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact
    25381 gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta
    25441 agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc
    25501 acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca ggtgggtact
    25561 atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg
    25621 aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc
    25681 attgaaggcg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg
    25741 aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg
    25801 aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag tgctcgacag caaaccgtct
    25861 gtgctggcac tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct
    25921 ccctcaaccc cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac
    25981 aactccatac atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc
    26041 cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat
    26101 ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga
    26161 tgcccgtggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg
    26221 tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca
    26281 tctgcacggt gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg
    26341 cgtaattggc ctgacaggtg aaccagagaa cctgcgtaaa tatggcgaac tggtctgcat
    26401 gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ctgttggcgc aggatagccg
    26461 tttgcgggaa gaactggtga tgaacctgat tcaggctgag gagaatactc ccgcacttac
    26521 tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtggtgg ctattgttga
    26581 ggtcgacagc ggtcagcttg gcgtggacag cgcaatggcg gagttacaac aactgcaaaa
    26641 tgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt
    26701 ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg
    26761 agttgaacaa ctgattaccc gcatgaaaga gtacggccag ttgcgttttc gcgtttcgct
    26821 gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac
    26881 gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat
    26941 gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaatgaac tggcgcgacc
    27001 gctggcgcgg ctgaaagcga tggacaataa tggcttgctg cgacgaacgc tggcggcgtg
    27061 gtttcgccac aatgtgcaac cgctggctac gtcaaaggcg ctgtttattc atcgtaatac
    27121 tctggagtat cggcttaatc gtatatcgga actgaccggg cttgatttgg gtaattttga
    27181 tgacaggttg ctgctgtatg tggcgttgca gctggatgga gagcggtagg ttatgcgtta
    27241 aggtcgaggc ggcgctggct cattcccctc agcccgaagg gtagagggga atgtccgagt
    27301 taaccgtcag ttcggcatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg
    27361 atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga
    27421 tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga
    27481 ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt
    27541 ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc
    27601 tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt
    27661 tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat
    27721 gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc
    27781 gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct
    27841 ttattgtggt gatattc
//
LOCUS       DAFAVF010000050        27686 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFAVF010000050 DAFAVF010000000
VERSION     DAFAVF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.050"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            164..325
                     /locus_tag="LGK42_002078"
     CDS             164..325
                     /locus_tag="LGK42_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658328.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(409..1644)
                     /locus_tag="LGK42_002079"
     CDS             complement(409..1644)
                     /locus_tag="LGK42_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBK2658329.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1791..1867)
                     /locus_tag="LGK42_002080"
     tRNA            complement(1791..1867)
                     /locus_tag="LGK42_002080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1831..1833),aa:Pro,seq:tgg)
     gene            complement(1910..1996)
                     /locus_tag="LGK42_002081"
     tRNA            complement(1910..1996)
                     /locus_tag="LGK42_002081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:Leu,seq:cag)
     gene            complement(2017..2092)
                     /locus_tag="LGK42_002082"
     tRNA            complement(2017..2092)
                     /locus_tag="LGK42_002082"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:His,seq:gtg)
     gene            complement(2151..2227)
                     /locus_tag="LGK42_002083"
     tRNA            complement(2151..2227)
                     /locus_tag="LGK42_002083"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2191..2193),aa:Arg,seq:ccg)
     gene            complement(2330..3715)
                     /locus_tag="LGK42_002084"
     CDS             complement(2330..3715)
                     /locus_tag="LGK42_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2658330.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3906..4646)
                     /gene="rffM"
                     /locus_tag="LGK42_002085"
     CDS             complement(3906..4646)
                     /gene="rffM"
                     /locus_tag="LGK42_002085"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBK2658331.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4649..6001)
                     /gene="wzyE"
                     /locus_tag="LGK42_002086"
     CDS             complement(4649..6001)
                     /gene="wzyE"
                     /locus_tag="LGK42_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBK2658332.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5998..7077)
                     /gene="rffT"
                     /locus_tag="LGK42_002087"
                     /gene_synonym="wecF"
     CDS             complement(5998..7077)
                     /gene="rffT"
                     /locus_tag="LGK42_002087"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBK2658333.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7074..8324)
                     /gene="wzxE"
                     /locus_tag="LGK42_002088"
     CDS             complement(7074..8324)
                     /gene="wzxE"
                     /locus_tag="LGK42_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBK2658334.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKATLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8326..9456)
                     /gene="rffA"
                     /locus_tag="LGK42_002089"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8326..9456)
                     /gene="rffA"
                     /locus_tag="LGK42_002089"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBK2658335.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9461..10135)
                     /gene="rffC"
                     /locus_tag="LGK42_002090"
                     /gene_synonym="wecD"
     CDS             complement(9461..10135)
                     /gene="rffC"
                     /locus_tag="LGK42_002090"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBK2658336.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10113..10994)
                     /gene="rfbA"
                     /locus_tag="LGK42_002091"
     CDS             complement(10113..10994)
                     /gene="rfbA"
                     /locus_tag="LGK42_002091"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK2658337.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11013..12080)
                     /gene="rffG"
                     /locus_tag="LGK42_002092"
     CDS             complement(11013..12080)
                     /gene="rffG"
                     /locus_tag="LGK42_002092"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK2658338.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12080..13342)
                     /gene="wecC"
                     /locus_tag="LGK42_002093"
     CDS             complement(12080..13342)
                     /gene="wecC"
                     /locus_tag="LGK42_002093"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBK2658339.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13339..14469)
                     /gene="wecB"
                     /locus_tag="LGK42_002094"
     CDS             complement(13339..14469)
                     /gene="wecB"
                     /locus_tag="LGK42_002094"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBK2658340.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14525..15571)
                     /gene="wzzE"
                     /locus_tag="LGK42_002095"
     CDS             complement(14525..15571)
                     /gene="wzzE"
                     /locus_tag="LGK42_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBK2658341.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15583..16686)
                     /gene="wecA"
                     /locus_tag="LGK42_002096"
     CDS             complement(15583..16686)
                     /gene="wecA"
                     /locus_tag="LGK42_002096"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBK2658342.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16926..18185)
                     /gene="rho"
                     /locus_tag="LGK42_002097"
     CDS             complement(16926..18185)
                     /gene="rho"
                     /locus_tag="LGK42_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK2658343.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18270..18371)
                     /gene="rhoL"
                     /locus_tag="LGK42_002098"
     CDS             complement(18270..18371)
                     /gene="rhoL"
                     /locus_tag="LGK42_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBK2658344.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18512..18841)
                     /gene="trxA"
                     /locus_tag="LGK42_002099"
     CDS             complement(18512..18841)
                     /gene="trxA"
                     /locus_tag="LGK42_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBK2658345.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18972..20237
                     /gene="rhlB"
                     /locus_tag="LGK42_002100"
     CDS             18972..20237
                     /gene="rhlB"
                     /locus_tag="LGK42_002100"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBK2658346.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20371..21855
                     /gene="gppA"
                     /locus_tag="LGK42_002101"
     CDS             20371..21855
                     /gene="gppA"
                     /locus_tag="LGK42_002101"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBK2658347.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21902..23923)
                     /gene="rep"
                     /locus_tag="LGK42_002102"
     CDS             complement(21902..23923)
                     /gene="rep"
                     /locus_tag="LGK42_002102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBK2658348.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24140..24589
                     /locus_tag="LGK42_002103"
     CDS             24140..24589
                     /locus_tag="LGK42_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK2658349.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24788..25069
                     /gene="ppiC"
                     /locus_tag="LGK42_002104"
     CDS             24788..25069
                     /gene="ppiC"
                     /locus_tag="LGK42_002104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBK2658350.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25156..26631)
                     /gene="ilvC"
                     /locus_tag="LGK42_002105"
     CDS             complement(25156..26631)
                     /gene="ilvC"
                     /locus_tag="LGK42_002105"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBK2658351.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26781..27674
                     /gene="ilvY"
                     /locus_tag="LGK42_002106"
     CDS             26781..27674
                     /gene="ilvY"
                     /locus_tag="LGK42_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBK2658352.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
BASE COUNT         6867 a         7576 c         6891 g         6352 t
ORIGIN      
        1 ggattgaaaa cgctctcctg attttccaat tcattttctg gataaataaa taatttattt
       61 ttgtcactat tatttatgta atcatcctgt cagggagagg gatctcaatt atcaatgctt
      121 aattacgtca tcattttgat gacatgaaac cggagcattt tgcatggaat tttcgttttc
      181 acccaaactt tttgttgttg ctgtcgccgc cgctcttcct ctcatggcca gcgcagcaga
      241 taccccgtca actgccaccg cacgcaaagg ctttgccgga tacgatcacc caaaccagta
      301 tccacagaac gctgcaattt attgaatttg cacgatcatg taggccggat aaggcgttta
      361 cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg ccgctttttt acttattcac
      421 caccagcaaa tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca gcaaatcagc
      481 cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg cacacaaata
      541 attcagcccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc agcctcccca
      601 gcacgctttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa atttatcttc
      661 accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct gatgcatatt
      721 ccccagccga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc cgttgtgctc
      781 aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg gcgcaccgac
      841 aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat
      901 ccagtggtcg aatatcgcca ccagaaattc accaaattca tcggggcgca ccgaccattc
      961 cgtcagttcc ccctgaatat caccaggcgc atgtagtttc agtccatcgc tggcagctgt
     1021 ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc ccgcatcgca
     1081 caaaaaatca taaacctgca acggttgctg cgcgctggtg cggttgacgc agaccagcac
     1141 gttatagtcg acatgatgtt tttgcagcag cgtcagggca cgcatcacca gcttatgcgt
     1201 tggtctgcca cctttggtca cgcgatattg attgtggatc tcagccggac cgtccagcga
     1261 taacccaaca agaaaatgat tttctgccag aaatgcacac catttatcat cgagcagcac
     1321 gccgttagtc tggaagctgt tacttatctt cctgccagca ccgtatttcg cctgtaactt
     1381 tacggcacgg cggtaaaaat ccagcccgag taacgttggt tcgccgccct gccaggtaaa
     1441 agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg cttccagcgt
     1501 gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt cgagataaaa
     1561 acagtagtca cagttcagat tacaatcgga accactcggt ttcgccatca catgaaaagc
     1621 acgcgttgga acctgttgca gcatggtcac tccttgcctg aagtcatcct tcaatacgtt
     1681 aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc gaagggaggg
     1741 tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga
     1801 gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca agctgcgcta
     1861 ctcgccgaaa tactgctttt tgaattttta gttcaattct ttaaagtcgt ggtgcgaggg
     1921 gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca
     1981 attccgccac cttcgcattg tcacaacttc taataatggg gtggctaatg ggattcgaac
     2041 ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc accactacaa
     2101 atcttgttac gcggtattac taccaccgca gctcaagcgc cgggactaaa tggcgcgccc
     2161 gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc agctgagcta
     2221 cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct ctgtctagtc
     2281 cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct tattcctcca
     2341 gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc gtcaccgcca
     2401 gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag tacatgccga
     2461 tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac aggattgagc
     2521 ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc tggcttatca
     2581 atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg tagacaaaca
     2641 cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga atagcaatag
     2701 atactgccac acccgcaacg ggtacgccgt gacgggaaac tttcgccatc gccgccggta
     2761 actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg ccgctgttac
     2821 agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct gcggtaatac
     2881 cgattttggc aaaagtcagt acgaacgggc tgccgttgct gcctatttca ttccacggga
     2941 agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc cacagcacct
     3001 tgcctacggc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca gtaatgccaa
     3061 tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg aaccctttcc
     3121 agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc gactgcccgc
     3181 cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg ataatcgtgg
     3241 tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc gccgccagat
     3301 tcgccaacgc caccagcgcc actgcgatca atgcgggtat ccactgcgcc atctccggga
     3361 accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc gccatccaca
     3421 taaaccaata agaccaggcg gtgagataac caaagaacgg gctcatataa cgatgcgcat
     3481 aaacggcgaa cgaaccggta accggttcga ggaacaacat ttcgcccatt gaacgcatga
     3541 tgaaaaagac gaatagcccg gcgatgatat aggccaacaa tacggatggc ccggcccatt
     3601 tcaaggtact ggcggccccc ataaacaggc cgacgccaat ggtgcccccc agggcgatga
     3661 gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct gccataaatc
     3721 ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt acatcgagtg
     3781 ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa tttgaatgct
     3841 ttgtgtaata aaaaagcagg caggcgacga agtgaccact ccgtcgcttt acaaagagag
     3901 gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg aagctgacgc
     3961 ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag cgtttgccag
     4021 attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc aacgcccata
     4081 tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg ctttggcgat
     4141 cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa cagcgcctga
     4201 cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt ccattggttg
     4261 cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag aaataccggc
     4321 gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc ggcaacgcgg
     4381 gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat gccatccgca
     4441 tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc aatagtcagc
     4501 attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc agcaaacaga
     4561 taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg cagcgtatag
     4621 gttggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg agcgatgatt
     4681 ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacaacagt tttgcgatca
     4741 tcagacatgc gccgaagacc acgataaaaa agaccacgcg tgagacaaac gaatccagcc
     4801 cttcacgcgc cagcacgatc atattgaaga tcgctccaaa gcagaaactg tgcaatatcg
     4861 cagccttata gcgattaggc tcgcggttgc ccagctcata cagccagtcg aaccatttga
     4921 tgatcagacc aaccacgatc gccccgagcg ggatgaacaa cgcgccgccc atcaccacca
     4981 gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc acttcccagg
     5041 taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc caggaaggga
     5101 taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg ttgtcgtagt
     5161 tctgcaacag caacgccaga ttctcccacg gcgagaaggt gtcgcgagtg agatagagaa
     5221 acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc gccagccaga
     5281 acatgccaac aatccccagc acgcccgccg ccgccagcat ccacaacgaa atccagccgc
     5341 gaataatgcc aataaacagg aagatagcga atgcgatgat gatattagcg cgagtgccgc
     5401 cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa aacagccacg
     5461 ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga
     5521 agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag gagttaagcc
     5581 ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc gcgatgccca
     5641 tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg
     5701 gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa accgcgtaga
     5761 agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa
     5821 agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc aaaaacagca
     5881 atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg
     5941 caataaacag cgtgcagagc agccagacaa caaacaggcc actgaattgc agcagactca
     6001 tgcgacctcc ccggcggcaa tcgccaacgc ccgctgccag ccttgtagat agttagggct
     6061 aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt cacgcacaat
     6121 atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg tcatatcctg
     6181 ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca gtaagcaaag
     6241 cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact gacgaagtag
     6301 cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt cttcgctgaa
     6361 taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag gcggatatcc
     6421 catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc acaaggcagc
     6481 aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt tcccttcacg
     6541 ttgccgatcg ttcgccatcg tattgagaga agggtccatc cgcgtcggga agtacagcag
     6601 ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcgccgc gggtggcaaa
     6661 tacacagccg actcgctttt gcgccaggcg acgtagtggg taaaaaagct tatatctcaa
     6721 gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact ggctgggctt
     6781 aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt ggaagaaaaa
     6841 acgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg attttttccc
     6901 agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt ccttgccaac
     6961 aaccataaac tcgcgcgcgt gctcgctcgt cgcagccagc gcgtcattga aaaaccgcaa
     7021 aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta cagtcatgcc
     7081 cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat ataagttgcc
     7141 atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc
     7201 atcaataaag tgaactggct gatttccgcc agaatataaa accgcaatgt cgctttggcg
     7261 atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac taactgccag
     7321 gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcagccagat agcaaaatca
     7381 cgcagcagcc agacggtaaa actcgccgcc gccactgccg gtaagacgaa tttcagcgat
     7441 ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa
     7501 tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact gctcacccct
     7561 tgccagatcc ccacttcatc ccagctatac tgcgccgcga gcagtttacg catcatgatg
     7621 taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt gctcaactgc
     7681 cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac accacgtttg
     7741 atcaacatga tggcggcagg aattaccacc agcgcgggaa tcagcgccag acccagcaac
     7801 gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc aatcaagctg
     7861 ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat cagcgccagt
     7921 aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac cagcccctga
     7981 tagtcggtat taccaaacaa tccctggctg attggcgcag ctgccagcac aaaaaccagc
     8041 gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac gcggcgcagc
     8101 tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa gatgccagct
     8161 ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag cccaagcccc
     8221 gccggaccaa atgacacagc cagcaactta ccgaccagta acccggcacc aatcttgacc
     8281 agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg agaagtagtt
     8341 caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga acagcggcag
     8401 gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga actcaccaaa
     8461 gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc
     8521 ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt taatgtagaa
     8581 catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac gcccggcttt
     8641 cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt gctggttgat
     8701 acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg acatcaaata
     8761 gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga actggctgcg
     8821 gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat cgttaatcag
     8881 cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt tcatggaagc taaagcagcc
     8941 aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc
     9001 atcttctacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt ccatttcgca
     9061 ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc
     9121 aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc
     9181 gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca tgatcacttc
     9241 atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg tgcaggacgg
     9301 cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc agcgacgggt
     9361 aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat agtcgagttc
     9421 ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat aaccagtacg
     9481 cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg gtgttgccca
     9541 tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt agcgccgttt
     9601 gcatcagctc agcacctaca ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat
     9661 tgagttcccg taaagagaca taaccacgaa taccgccgga cgccgcacgt aaaatcagac
     9721 attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa aagcgtctgc
     9781 tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta
     9841 actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta tcactgacat
     9901 tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc agctgttgca
     9961 gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt gaccacggcg
    10021 ctaacacgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg gcgctgttaa
    10081 caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg ggcacgaagt
    10141 aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc acgcttcaca
    10201 ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc aatcttaaag
    10261 ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct gtcgtgagtg
    10321 ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg
    10381 aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg cttcacctgc
    10441 tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc ccagtttgac
    10501 ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc aaactccacc
    10561 acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc ttccgtgcgc
    10621 gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt atcgcccagc
    10681 accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc ctgcgccaga
    10741 ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact accatcgccc
    10801 agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat ttcgcggata
    10861 ccggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta aatcggcaac
    10921 agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg
    10981 ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa acgctcgccc
    11041 tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata ccactgcacc
    11101 gttttacgca ttccactttc aaaggtttcc tgtggcagcc aaccaagttc acgggcaatt
    11161 ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac aaaggtgatc
    11221 aagtcacgat agtgcgccac gccgtgcggc ttgttcggag ccagttcttc cagcagctcg
    11281 caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat attataggtt
    11341 tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac
    11401 agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc cgccagcgcg
    11461 ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta gttattcgag
    11521 cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac cagatggtcg
    11581 ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt ttcggtgaag
    11641 aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg atgaaaacgg
    11701 aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc ttcaagcaat
    11761 gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat agaacggtca
    11821 acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt gaatacgcgt
    11881 gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc gctttgcgcg
    11941 accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac taccaccgcg
    12001 tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc ggcaccacct
    12061 gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca tactgctgat
    12121 ggacattgtc gccattgata actttgaact gactatgatc gaccagcatc accagcacgt
    12181 ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc agttttttcg
    12241 gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac tgggcgatca
    12301 gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt ttaaacgcca
    12361 gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagcagcc aggcaatcag
    12421 ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc acttcgcgtg
    12481 cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac ggatcaacag
    12541 caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga gggtgacgat
    12601 tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc agcgacaatt
    12661 cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta cacatttccg
    12721 ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac agttcgctgg
    12781 cgcgggccga acaaaccggc gtcataccac caatcacgcg atcgttttta atcagctcga
    12841 ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg tccgcctgct
    12901 cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat tctgccatct
    12961 tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct tttttcagca
    13021 ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca tgatcgcctt
    13081 taaacggcgt aggtacagca atcagccagg catccgcttc aactggcgtc gtgctcgctc
    13141 gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggt tcgacgatat
    13201 ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct
    13261 gtttttgccg tgaggcaaac gcagctgctg taggcagccc gatgtaaccc agtccgataa
    13321 cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt ccagaatgcg
    13381 agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagctt gatattcgtt
    13441 ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg tgcctaccag
    13501 acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac
    13561 cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca aaatcagcca
    13621 ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca gaatgacatt
    13681 tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga gatgcaccgg
    13741 atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcgt ggcagatttc
    13801 ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca tctttttatc
    13861 ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtgtcgc tgctcatcac
    13921 ctgatcacgc acccataaca gtgcatcaat gactgtatta ccagtaatga agattcggct
    13981 atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag agaagtgata
    14041 catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg aatagagatc
    14101 gcccgtgcgt aaaccagcct caacgtgacc aacaggaata cgctgataaa acgccgccag
    14161 gctggttgcc agcgtcgttg ttgtgtcgcc gtgaaccagc acgacgtctg gtttgaactc
    14221 ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca ggccctgtcc
    14281 tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca gcacctgatc
    14341 gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag gatcttttgc
    14401 caacgcatgc accaacggag ccatcttgat ggcttccggg cgcgtaccaa atacagtcag
    14461 tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctcg cagcagcagt
    14521 gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc cccccgacaa
    14581 tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc ggttcttccg
    14641 gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca acattcaggg
    14701 tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg acggcctgta
    14761 aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct gaatcaggta
    14821 attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca gcaattttca
    14881 gcgcctgctc aatgctgttc atccggcggt cgtagatggc tttcgccacc tcttcctgac
    14941 gcttcacctg agctttcatc tggatggtgc gtgccgccca tgcgcctttc agctcatcat
    15001 tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac aggttattag
    15061 cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg gtaaagtctc
    15121 cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg gctttgctgt
    15181 tgcccaccat ccgctgcttg taatagtcgg tttgcagcca gaactctctg cgggtatccc
    15241 acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat ggttggtcgg
    15301 cagaagccat gtttgaacgg gcatccaggt tacgcaaaaa ttgctgctgc gagtaatatc
    15361 cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgcgctccac tcttgtcgag
    15421 caaaaaaagt atacgccagc gcgattaacg caaacgccag ccccatgcca ataatccata
    15481 gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt tcagcgtctt
    15541 cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg ttaaattggg
    15601 gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt
    15661 ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga gcaccagcat
    15721 gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag cgagcagtgc
    15781 tgcggcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac gcatgatcaa
    15841 atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca ggcgacggta
    15901 cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg taaccgggct
    15961 gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg taaaaccaat
    16021 cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga taccaaggtt
    16081 aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg cgaggctggt
    16141 ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg acaacccgcc
    16201 cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc agacggcaaa
    16261 tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa agatataacc
    16321 caggctactg agataaagtt tgccgaacac catcataaca atgccaacag cggcctgtat
    16381 ggtggcacgg attttcacgc tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag
    16441 cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga caattccgaa
    16501 cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca atccctggtg
    16561 acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct tacgggcaaa
    16621 aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca ctgtcagtaa
    16681 attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg aagaccttta
    16741 ttcagaaggg aaagtccgtt ccgaacccac cagccctgtg caaaaattaa ccatttgata
    16801 tgcaaattgc ataacatgcc agcaaattcc aggaattttc ctaacatcgc cagcacggca
    16861 ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt tggcataaga
    16921 caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt catggccagt
    16981 ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg
    17041 atccacattt tctgcagttc ttcctgagtc gtgagcagct cttctttacg ggtaccggaa
    17101 cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt
    17161 tccatgttgc ctgtaccttt aaactcttcg taaataactt cgtccatttt agaaccggta
    17221 tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt acgcgccgca
    17281 ccgaagaagc gtttcggacg atgaagggca ttggcgtcca caccaccggt caacacttta
    17341 cctgacgccg gaacaacagt gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga
    17401 atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac catttccgca
    17461 acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc accttttacc
    17521 agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac catcagcaca
    17581 caatccgggt ggttgtaagc aatgctctga gcaatgttct gcagcagcat agttttaccg
    17641 gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga tgccagatcc
    17701 agtacgcgag cggttaaatc ttcagtagaa ccgttaccac gttccatacg cagacgagag
    17761 tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg
    17821 tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc tttcggcggg
    17881 cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat ttggctaggg
    17941 gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg gaggaaacca
    18001 aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc actctttgcg
    18061 tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tacgagccag gttttccagc
    18121 cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa ttcggtaaga
    18181 ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt tgtgaatggt
    18241 atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg ctgcctggtc
    18301 acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc agaaatctgt
    18361 tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca aacttaataa
    18421 ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc gtccagtttt
    18481 tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg aggaactctt
    18541 tcaactgacc tttagacagt gcacccactt tggttgccgc cacttcaccg tttttgaaca
    18601 gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg ttttgatcga
    18661 tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca tccagaatcg
    18721 gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg atcgccccgt
    18781 ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca
    18841 tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt aacattaacc
    18901 aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt aagctgatat
    18961 tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac ttcgccctgc
    19021 atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg cccattcagg
    19081 cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta
    19141 ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct catcctgcga
    19201 ttgccgatcg caaggtgaat caaccacgtg ccttaattat ggcaccgacg cgtgaacttg
    19261 ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc taccggcctg aagctgggtc
    19321 tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc ggcgttgaca
    19381 ttctgattgg cactacgggg cgtttaattg actacgccaa gcagaaccac attaacctcg
    19441 gtgccattca ggtggtggta ctggacgaag ccgatcgcat gtacgatctg ggctttatta
    19501 aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt
    19561 tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg aacaatgccg
    19621 aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa gagcttttct
    19681 acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag
    19741 acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg ggccacctgg
    19801 cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc
    19861 gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc gacgttgccg
    19921 cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta cccgatgact
    19981 gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc ggtcactcta
    20041 tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgagacc tatattggtc
    20101 actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg ccaaaaccgc
    20161 tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc
    20221 gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg
    20281 caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa gaatgcagcc
    20341 aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct gtatgcagcc
    20401 attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc
    20461 cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat
    20521 gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt
    20581 ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt acgtcttgcc
    20641 gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg
    20701 atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac aactggcggt
    20761 gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt aaccggcacg
    20821 ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc
    20881 tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc
    20941 gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt
    21001 gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc
    21061 attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg tcggctggag
    21121 gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg
    21181 atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg
    21241 ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc
    21301 cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc gcagcgcgta
    21361 gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct tgaagcaata
    21421 agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttt
    21481 aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc
    21541 cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc
    21601 tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta
    21661 ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca
    21721 ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat
    21781 ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg
    21841 ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg
    21901 attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt
    21961 tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct
    22021 gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg tactgacggc
    22081 gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca
    22141 gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc aaaaaccctt
    22201 cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg tgcagagtca
    22261 tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag
    22321 taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca
    22381 tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg
    22441 ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc agatcgcgca
    22501 ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag tgagtaaagc
    22561 gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg
    22621 cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtcgccg
    22681 ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt
    22741 tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg
    22801 taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca aacactcgcg
    22861 actgatggtt accgcgataa agaatagcgt aatctttgta ctgcgtttta ttgacgaagt
    22921 gatgcgcaat cagttcacca gtgacgcgct cggcttcatg ttcttcgtta ttcgcgctta
    22981 aaactttaag ctctgtgccg taacccagtt cggagaacag acgcttctca aagacgtgcg
    23041 ggttattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag cggtagttct
    23101 gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgtg
    23161 gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg gtaaagcgcg
    23221 cgcggctgcc caccagcagt ttcaccagct catactggct ggtgtttgtg tcctgatact
    23281 catccaccag cagatagcga attttgttct gccagcgctc gcggacttct tcattgcgtt
    23341 gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg cacgctttca
    23401 ggtgtgcatc atacagccca taacaatggg caaaaatacg atcccgctcg ccctttgcct
    23461 ctgccgccgc ctgcgccgga gttttgagat cgttcttcca gttggagatg gtcgaaatca
    23521 gctgttgcag gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag
    23581 caagctgatc ggtatcgtca aacagcgaaa agttcgcctt catcccaagc gccgcatact
    23641 cgcgtttgat gatatccaac cccagcgtgt gaaaagtgga gatcatcagc ccgcgcgcct
    23701 ctttgcgacc cagcgtctgc ccgacacgct ctttcatctc gcgcgccgcc ttattggtaa
    23761 aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt
    23821 tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg
    23881 taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc
    23941 ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct
    24001 aaccggttgg ctgcggatgt ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg
    24061 aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt
    24121 actaaggaga ggtttactcg tggcgattaa tttcagccct aaggttggtg aaatacagga
    24181 atgcaacttt ggtaactatc ctgttatcca gaatggacag ttcagcacca cgtattacga
    24241 tggccgcatt cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca acggaaaaat
    24301 taatggcaac gcatgtatcg tcgttccatt atcaaccact cgcgatcatg acaagttaaa
    24361 tcgtggaatg catgttgaaa tagccagtaa tgttatcaat gtcctgcaat tctttgatca
    24421 gcagattcgt tgggccaaag ccgacctggt acaacaagtc agtcgaaacc gattaaatag
    24481 agcaaggact tacagagggt atcttaatca atgtttgcca catgagttgg ttgcagacat
    24541 acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac aataaaataa
    24601 ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc cttgagggac
    24661 ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagcccctc cctgactgac
    24721 aattacgtta gcatctgcgg atcatgctaa aatcgccgcc tgacaattat tcactcaagg
    24781 cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc
    24841 tctggatctt ctcgagcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca
    24901 ctccatttgc ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat
    24961 ggttccggcg ttcgataaag ttgtgttctc ttgtccggta ctggagccga ccggcccgct
    25021 gcacacccag ttcggttacc acatcattaa ggtgctgtac cgcaactaat agcaaggcct
    25081 tctccaggag aaggccttga gtgttttctc cctctccctg tgggagaggg tcggggtgag
    25141 ggcatcagcg cgcacttaac ccgcaacagc aatacgtttc atatctgtca tatagccgcg
    25201 cagtttctta cctacctgct caatcgcatg gctgcgaatc gcttcgttca catcacgcag
    25261 ttgcgcgtta tctaccgcgc cttccggaat agctttaccc aagtcgcccg gttgcagctc
    25321 tgccataaac ggtttcagca acggcacaca agcgtaagag aacagatagt taccgtactc
    25381 agcggtatca gagataacca cgttcatttc gtacagacgc ttacgggcga tggtgttggc
    25441 aatcagcggc agctcgtgca gtgattcata atatgcagac tcttcaatga tgccggaatc
    25501 gaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc
    25561 tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc
    25621 ggttttgccg gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccgc
    25681 catcataccg gaggagaatt cgccggagat gatgtcgtcc atatgtttct ggaacagggg
    25741 tgccatgatc tctttcagct gttcagacag cgcgtaagca cgcagtttcg ccgggttaga
    25801 gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc gcttcggtga tggtttccca
    25861 accgaactga attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc
    25921 gaagcacagc agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag
    25981 gtcagatttc acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt
    26041 tgctgccgcc catgctttag cgatcgccat accttcgcct ttcggatcgt tttccgggtg
    26101 aacggcaatc agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc
    26161 agggcatttc ggcgcaacca tgactacggt gatatcttta cggatctgct cgcccacttc
    26221 gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac
    26281 ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg
    26341 cgggatcagt tcttcgtaag taccaacttt aaaaccgttt tcggtcgctt tacgccagga
    26401 cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc
    26461 acgcatgttc aggccctggt tcagcccctg tgcgccacag ccgacgataa ccactttttt
    26521 accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc cccataaagc gacatttgcc
    26581 cagctgtgcc agctgctggc gcaggttcag tgtattgaag tagttagcca tggtgattcc
    26641 tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt cactatatga
    26701 caggaaattt attgcggaaa ttgatatatt cacaacgtca cattgcaatt tctgcaacgt
    26761 caacattgag ggctgcgcca ttggatttac gcgatctgaa aaccttcctg catctggcgg
    26821 aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt tagcccatcc acgctctcac
    26881 ggcagattca gcgcctggaa gaagatcttg gtcagccgct atttgtgcgc gataaccgca
    26941 cggtaacgct gaccgaagcg ggcgaagagt tacgcgtttt cgcccagcaa acattgttgc
    27001 agtatcagca actgcgccac accatcgatc agcaagggcc gtcgctctct ggcgagttac
    27061 atattttctg ctcggtgacc gccgcttaca gccatctacc gccgattctc gaccgcttcc
    27121 gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg tgatgcggca gatgcaatgg
    27181 aaaaagtggt cacgggagaa gcggatctgg cgattgccgg gaaaccggag acgctacccg
    27241 gcgctgtggc gttttcgatg ctggagaatc tggcagtagt gctgattgcc cccgcgctgc
    27301 cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga ctggtcaacg gtgccgttta
    27361 ttatggccga tcaggggccg gtacgccgcc gcattgaact gtggtttcga cgcaataaaa
    27421 tcagcaaccc gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg
    27481 cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct ggaaaacagc ccggagcctg
    27541 tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa aacgccgttt gaactgggcg
    27601 tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga ggcgttctgg aggattttgc
    27661 cgaaccacaa atgacgttgt cgcgcg
//
LOCUS       DAFAVF010000051        26256 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFAVF010000051 DAFAVF010000000
VERSION     DAFAVF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.051"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="LGK42_002107"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="LGK42_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK2658353.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="LGK42_002108"
     CDS             complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="LGK42_002108"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBK2658354.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRVGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="LGK42_002109"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="LGK42_002109"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBK2658355.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="LGK42_002110"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="LGK42_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBK2658356.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="LGK42_002111"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="LGK42_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK2658357.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="LGK42_002112"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="LGK42_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBK2658358.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="LGK42_002113"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="LGK42_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBK2658359.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="LGK42_002114"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="LGK42_002114"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBK2658360.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="LGK42_002115"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="LGK42_002115"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBK2658361.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9972..10337
                     /locus_tag="LGK42_002116"
     CDS             9972..10337
                     /locus_tag="LGK42_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBK2658362.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /locus_tag="LGK42_002117"
     CDS             10487..11005
                     /locus_tag="LGK42_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBK2658363.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="LGK42_002118"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="LGK42_002118"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBK2658364.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /locus_tag="LGK42_002119"
     CDS             12237..12719
                     /locus_tag="LGK42_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBK2658365.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="LGK42_002120"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="LGK42_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK2658366.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="LGK42_002121"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="LGK42_002121"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK2658367.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="LGK42_002122"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="LGK42_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK2658368.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="LGK42_002123"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="LGK42_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK2658369.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14935..16296)
                     /gene="ffh"
                     /locus_tag="LGK42_002124"
     CDS             complement(14935..16296)
                     /gene="ffh"
                     /locus_tag="LGK42_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK2658370.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16463..17254
                     /locus_tag="LGK42_002125"
     CDS             16463..17254
                     /locus_tag="LGK42_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBK2658371.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKTL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17275..18561
                     /locus_tag="LGK42_002126"
     CDS             17275..18561
                     /locus_tag="LGK42_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK2658372.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18616..19209)
                     /gene="grpE"
                     /locus_tag="LGK42_002127"
     CDS             complement(18616..19209)
                     /gene="grpE"
                     /locus_tag="LGK42_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK2658373.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19332..20210
                     /gene="nadK"
                     /locus_tag="LGK42_002128"
     CDS             19332..20210
                     /gene="nadK"
                     /locus_tag="LGK42_002128"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBK2658374.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20296..21957
                     /gene="recN"
                     /locus_tag="LGK42_002129"
     CDS             20296..21957
                     /gene="recN"
                     /locus_tag="LGK42_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK2658375.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22106..22447
                     /gene="bamE"
                     /locus_tag="LGK42_002130"
     CDS             22106..22447
                     /gene="bamE"
                     /locus_tag="LGK42_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBK2658376.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22509..22799)
                     /locus_tag="LGK42_002131"
     CDS             complement(22509..22799)
                     /locus_tag="LGK42_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBK2658377.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22789..23226)
                     /gene="ratA"
                     /locus_tag="LGK42_002132"
     CDS             complement(22789..23226)
                     /gene="ratA"
                     /locus_tag="LGK42_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBK2658378.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23397..23879
                     /gene="smpB"
                     /locus_tag="LGK42_002133"
     CDS             23397..23879
                     /gene="smpB"
                     /locus_tag="LGK42_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK2658379.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24094..24456
                     /gene="ssrA"
                     /locus_tag="LGK42_002134"
     tmRNA           24094..24456
                     /gene="ssrA"
                     /locus_tag="LGK42_002134"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24662..25903
                     /locus_tag="LGK42_002135"
     CDS             24662..25903
                     /locus_tag="LGK42_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK2658380.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGSYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEALWEEVDIEAREWKIPAARMKM
                     NRDHTVPLSDEAIAILEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWADF
                     VMAADHGSMIEGGIKAMRLVG"
BASE COUNT         6698 a         6900 c         6177 g         6481 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgttcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcatcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaacg gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcaatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt gatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttccca
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccag
     3361 gcgtattgct ataagaggca tcagctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagtga gtcatacggg ggcaagctca cgccgccgat acgagtagag ctacaggccg
     3601 caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcat tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg agagggtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgctta gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agcagcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaccc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccga ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacggtcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccaac aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcatcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ctgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tggaagcact gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtattgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc actacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctacc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa
    12721 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtca
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt ccccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tgatttctgg agaaaatgca atcagcagtt aataactctg tcgcataagg tcatttagcg
    14941 accaggaaat cccggcggca tcatcccctt catgcttctc atcatcttcg ccattccgcc
    15001 tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac tgcttcagaa ggcggttcac
    15061 gtcctgcacc tgcataccgc aaccggcagc gatacgacgc ttacgcgaac ctttgatgat
    15121 ttctggctta gcgcgctctt tcatcgtcat cgagttgatg atggcttcca tacgcaccag
    15181 cactttatcg tccatctgcg acttaacatt atccgggatc tgccccatgc ccggcagctt
    15241 gcccatcagg ctagccatgc cgcccatatt tttcatctgg cgcagttgct caagaaaatc
    15301 gttgagatcg aagccgtcgc cttttttcag cttgctggct aatttctctg cctgcgcgcg
    15361 gtcaactttg ctttcgatat cttcgatcag cgacagtacg tcgcccatgc cgagaatacg
    15421 cgacgcgatg cggtccggat ggaacggctc cagcgcctca gttttctcgc caacaccgag
    15481 aaacttgatc ggtttgccag tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc
    15541 gccgtccact ttggtcaaca ctacgccggt aagcggtaac gcttcattga atgcttttgc
    15601 cgtattggcc gcatcctgac cggtcatggc gtcaaccaca aacagggttt caaccgggtt
    15661 aatcgacgca tggacttgtt tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc
    15721 agcggtatcc accagcagca cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt
    15781 aacgatatct accggcttct gaccaacatc agaagggaag aaatcaacgc ccacctgctc
    15841 tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa acgtcggcag aaaccaccag
    15901 cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt ttaccaacgc tggttgtttt
    15961 accggcacct tgcaggcccg ccatcagcac gaccgcaggc ggttgcgccg ccaggttcag
    16021 ggtctggttc tcttcgccca tcgccgcaac cagttcgtta cggacgattt tgacgaactc
    16081 ctgccccggc gtcaggctct tattaacttc atgaccaacc gctttctctt ttacgcgatt
    16141 gataaactca cgcactaccg gcagagcgac atccgcctcc agcagcgcca tgcgcacttc
    16201 gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca cggccactga tattgcgcag
    16261 cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg
    16321 gttggccgca atcgcgacac atcatcagta ttttgccgca gtataacatg aaggcgtctt
    16381 tgttgttatg caacggttgg agcagcgttc acctgacgct atactgcttc tctttcttat
    16441 tgctcaaact gtcgacatca ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc
    16501 cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat
    16561 ggctattatt tctgcggtca ttgcgctggt ctgccacgca atcgctctgg aagcccgcat
    16621 cctgcccgac ggtgatagcg gacaaaacct cagcctgctg aacgttggtt cattggtcag
    16681 tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc aatcgtggct ggttgctgct
    16741 acccattgtc tatgcctttg cgcttatcaa cctggcgctg gcgaccttca tgcccaatga
    16801 atacatcacc catctggaag caacgccagg gatgctggtg cacattggct tatcgctctt
    16861 ttcctatgcc acgctaatta tcgccgccct gtacgcgctg caactggcgt ggattgatta
    16921 ccaactgaag aacaagacgc tggcatttaa ccaggaaatg ccgccattga tgagtatcga
    16981 gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg ctaacgctca cgctttgcac
    17041 tggcctgttc tacatgcaca acttatttag catggaaaat atcgacaagg ccgtgctctc
    17101 tatcgtggcg tggtttgtct atattgtgct gctgtgggga cattatcatg aaggatggcg
    17161 tggacgccgc gtcgtctggt ttaacgttgc gggtgcagtc atcctgacac tggcctactt
    17221 cggcagccga attgtccagc agttaatcag ctaaacccag aaaaggagtt tcccctggaa
    17281 cacatttcta ctactacgtt gatcattatt ctgatcatca tggtggtcat ttcagcctat
    17341 ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct atcgtctgcg acatatggcg
    17401 aaacagggta atcgctcggc caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg
    17461 ataagcctgg tgttaatcgg caataacctg gtcaatattc ttgcctccgc gctcggcact
    17521 attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact
    17581 tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca ttgccgcgct gtacccggaa
    17641 aaagtcgctt atccgagtag ttttctgctg gctccgctgc aaattttgat gatgccgctg
    17701 gtctggttgc tgaatgctat cacccgtatg ctgatgcgca tgatgggtat caaaaccgat
    17761 atcgtggtta gcggctcttt gagcaaagaa gagttgcgca ctatcgtgca cgaatcacgc
    17821 tcacaaattt cccgtcgcaa tcaggatatg ctactgtctg tgctcgatct ggaaaagatg
    17881 accgttgatg acatcatggt gccgcgcagt gaaattatcg gtattgatat caacgatgac
    17941 tggaaatcga ttctgcgcca actctcccac tcacctcacg ggcgcatcgt gctctaccgt
    18001 gattcgctgg acgacgccat cagtatgctg cgagtacgtg aagcctggcg gttgatgtcg
    18061 gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg cggacgagat ctattttgtg
    18121 ccggaaggta cgccgctcag cacgcagttg gtaaagtttc agcgcaacaa aaagaaagtc
    18181 ggcctggtcg tcaacgagta tggcgacatt caggggctgg tgacggttga agatattctg
    18241 gaagagattg tcggcgattt cactacgtcg atgtcgccaa cacttgccga agaggtcacg
    18301 ccgcaaaacg acggttcggt gattatcgat ggcaccgcca acgtgcggga aatcaacaaa
    18361 gcctttaact ggcatctacc ggaagatgat gcccgcacgg ttaatggcgt cattcttgag
    18421 gcactggaag agatccctgt cgcaggcacc cgcgtgcgta ttggcgagta cgatatagat
    18481 attctcgacg tacaggacaa tatgattaag caggtaaaag tttttcctgt gaaaccgctg
    18541 cgcgagagtg tggcggagta acgaaaacgg ccctgcattc gcatgcaggg ccgttaatta
    18601 ttacgaaaac agaaattacg cttttgcttt cgctacagtt accatcgccg cacgaatcgt
    18661 acgaccattc agcgtataac ccttctgcat aatgcccagt acgttacctg gcgcaacgtc
    18721 atcagattcc accattgcga tagcctgatg cacattcggg tccagcggca cgttagtttc
    18781 ggcgatcact tcaacaccaa acttacgcac aacatccagc atcgacttca gcgtcagctc
    18841 aatgccttca accatcgcag acatatccgg gttagcttta tcagccactt ccagcgcacg
    18901 atccaggcta tcaatcaccg gcagcaattc gttgatgaat ttctccagcg cgaatttgtg
    18961 ggctttttca atatccagtt cagtacgtcg acgcaggttt tccatttcgg cttttacacg
    19021 caaaatgccg tcacgttcac gggtctgggc ttcagccagc tgagcttcga gattcgcaat
    19081 tttttcatcg cgcggatcca cctgctcagc agaagcttct ggctcaactg cctcaatctc
    19141 ttcgtgctga tccatgataa tttcttccgg ggcttgcccc tcaggcgttt tctgttcttt
    19201 actactcatg aatttctccg cgtttttttc gcattcatct cgctaacttc gcttattatg
    19261 gggatcagtt tcagggtttc aagggaagca ctcacattgt catcaatctt cgcaacaagg
    19321 acctcggaaa aatgaataat catttcaagt gtattggcat tgtgggacac ccacggcacc
    19381 ccactgcact gacaacacat gaaatgctct accgctggct gtgcacaaaa ggttacgagg
    19441 tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa gaatgtgaaa actggcacgc
    19501 tcgcggagat tgggcaacta gctgatctcg cggtagtcgt tggtggcgac ggtaatatgc
    19561 tgggcgcggc acgcacactc gcccgttacg atattaaagt tattggaatc aaccgtggca
    19621 acctgggttt cctgactgac cttgaccccg ataacgccca gcaacagtta gccgatgtgc
    19681 tggaaggcca ctacatcagc gagaaacgtt ttttgctgga agcgcaagtc tgtcagcaag
    19741 attgccaaaa gcgcatcagc accgcgataa atgaagtggt gcttcatcca ggcaaagtgg
    19801 cgcatatgat agagttcgaa gtgtatatcg acgagatctt tgcgttttct cagcgatctg
    19861 atggactaat tatttcgacg ccaacaggct ccaccgccta ttccctctct gcaggcggtc
    19921 ctattctgac cccctctctg gatgcgatta ccctggtgcc catgttcccg catacgttgt
    19981 cagcacgacc actggtcata aacagcagca gcacgatccg tctgcgtttt tcgcatcgcc
    20041 gtaacgacct ggaaatcagt tgcgacagcc agatagcact gccgattcag gaaggtgaag
    20101 atgtcctgat tcgtcgctgt gattaccatc tgaatctgat tcatccgaaa gattacagtt
    20161 atttcaacac attaagcacc aagctcggct ggtcaaaaaa attattctaa ttttacgcca
    20221 gcctctttac tgtatataaa accagtttat actgtacaca ataacagtaa tggtttttca
    20281 tacaggaaaa cgactatgtt ggcacaactg accatcagca actttgctat cgttcgtgag
    20341 cttgagattg attttcatag cggcatgacc gtaataactg gcgagaccgg cgcgggtaaa
    20401 tctattgcaa tagatgccct cggtctttgt ctcggtggtc gcgctgaagc cgacatggtg
    20461 cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt ctctgaaaga tacgccagcg
    20521 gctctgcgct ggctggaaga aaatcagctt gaagacgggc atgaatgttt gcttcgtcga
    20581 gtgatcagca gcgatggtcg ctcccgtggt ttcatcaacg gtacagctgt tcctctgtca
    20641 caactgcgcg aactgggtca gttgctgatt cagatccatg gtcagcacgc tcatcaatta
    20701 ctcaccaaac ctgagcacca aaaattcctg cttgatggct atgccaatga aacctctcta
    20761 ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa gctgccgtga cctcgcgcat
    20821 catcaacagt taagtcagga acgcgccgcc cgtgcggaac tgctgcaata ccaattaaaa
    20881 gaacttaacg aatttaatcc gcagcccgga gagtttgaac aaatcgacga agagtacaaa
    20941 cgtctggcga acagcggtca attgctgacc accagccaga atgcattggc attaatggcc
    21001 gacggtgaag acgcaaacct gcaaagtcag ctttacacgg ctaaacaact ggtgagcgaa
    21061 ttgattggca tggacagcaa actgtccggc gtacttgata tgctggaaga agctaccatc
    21121 cagattgctg aagccagcga tgaactgcgc cactactgcg atcgtctgga tctcgatccc
    21181 aaccgactat ttgaacttga acagcgcatc tcaaaacaga tttcgctggc acgtaaacat
    21241 cacgtcagcc ctgaggcatt gccacagtat taccagtcgc tactggaaga acagcagcaa
    21301 ctggacgatc aggccgactc acaagaaacg cttgcgctgg cggtaacgaa acatcatcag
    21361 caggcactgg aaatcgcgcg cgcattacac caacaacgcc agcaatatgc agaagaactt
    21421 gcacagctga tcaccgacag tatgcatgcg ctctcaatgc cgcatgggca gtttacgatc
    21481 gatgttaaat ttgacgagca tcacctgggc gctgacggtg ccgatcgtat tgagtttcgg
    21541 gtaaccacca acccaggtca gccaatgcag cctattgcca aagtcgcatc cggtggtgaa
    21601 ttgtcccgca tcgcactggc aatccaggtc atcacggcgc gtaaaatgga aaccccggca
    21661 ctgatttttg atgaagtgga tgtaggtatt agcggtccaa cagcggcagt tgtcggcaaa
    21721 ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg ttacccacct gccacaagtc
    21781 gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa ccgatggtgc gatgacagaa
    21841 acgcatatgc aatccctgaa taaaaaagcg cggttacaag agctggcgcg cctgcttggt
    21901 ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag aactgcttgc agcgtaaact
    21961 tttttcctgc ttcacggtca gagtaaacag caaaacgccg taagaccgga aagcaaaagg
    22021 ttttaaagtg atgaaaggtc tattatcatc ggcatattac agatgagcca cgtactgctc
    22081 gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg ctgactgctg cagcagcagt
    22141 actattgatg ttgaccgcag gctgttccac tctggagcga gtggtttacc gtcctgacat
    22201 caaccagggg aactatctga ccgctaacga cgtatccaaa atacgtgttg gcatgacgca
    22261 acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc gatccatttg gtacgaatac
    22321 ctggttctat gtcttccgcc agcaaccagg tcatgaaggt gtaacgcagc aaacgctgac
    22381 gctgaccttt aacagtagcg gtgtgttgac caatattgat aacaaacctg cgctgagtgg
    22441 taactaataa taaagttgct ctcaaagacg ttaaaaaagg tgctcaatga gcaccttttt
    22501 tctgtctgtt atttattcgc tgatttttct gctcgttgcc tgcgaagctc tttcggatcg
    22561 gcaatgagag ggcgataaat ctccacccga tcgccatcat gcacactatc gcttagtttt
    22621 gccggacggc tgtaaatgcc gactttgttt ttagttaaat cgatatcggt acgcaattcc
    22681 agcaagccac tggcgcgaat agcttcttca accgtcgcgc cctcctgcag cgtcacgcgc
    22741 tgcaggtact gcttctcagg tagcgcataa gccacctcaa cggcaatttt acctggcact
    22801 gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata ttagacgcca gctctttaaa
    22861 cacgcgacca aaggcgagtt caatcaactt attggtaaac tcaaagtcga gatgaaactc
    22921 gatacgacac gcctcctggc tcagcggcgt aaacttccat ccaccaatca atttcttgaa
    22981 cggcccatcc accagattca taagaatact ttggttactg gtcaactggt tgcgggtagt
    23041 aaacgttttg ctgatcccag ccttagagac atctaccgcc gcagtcatct gcccaggagt
    23101 ggactccaga atccgacttc cggtacaacc cggcaaaaac tgaggataag actgaacgtc
    23161 attcactaac tgatacattt gctccgcgct gtagggtacc agtgcggtcc ggctaatctg
    23221 aggcataaca atttccatca acaaaaatcc aacaaataat atcatttatc ctgctgtaaa
    23281 aaaaacgcta tcccggcgct gggtaacatc gggttcatgc taagatagag ccttgtcccc
    23341 cgcaggattg atatggggtg ttttcgattt cagattaccg atgattcacg acgcttatga
    23401 cgaagaaaaa agcacataaa cctggttcag cgaccatcgc gcttaacaag cgcgcccgtc
    23461 acgaatactt tatcgaagaa gagttcgaag cgggacttgc cctgcaaggc tgggaggtta
    23521 aatccctgcg cgcaggaaaa gccaatatca gcgacagcta cgtccttctg cgtgacggag
    23581 aggcatttct gtttggcgct aacatcacgc caatggccgt ggcctccacg catgtggtgt
    23641 gcgatcctac ccgtacccgc aagttacttc tcaaccaacg cgaactggac tcattgtacg
    23701 gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc cctgtactgg aaaaatgcct
    23761 ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca gcacgataaa cgttcagata
    23821 tcaaagaacg cgaatggcag gtggataaag cacgtatcat gaaaaacgcc caccgttaaa
    23881 cctgcactcc aattattgac cagttcctca ccgcgcctcc ctctccggcg gcgcgaatga
    23941 acatcttatt ggctatcaca tccgacacaa atgttgccat cccattgctt aatcgaataa
    24001 aaatcaggct acatgggtgc taaatcttta acgataacgc cattgaggct ggtcatggcg
    24061 ctcataaatc tggtatactt acctttacac attggggctg attctggatt cgacgggatt
    24121 tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg taaaaagccg caaaaaatag
    24181 tcgcaaacga cgaaaactac gctttagcag cttaataacc tgcttagagc cctctctccc
    24241 tagcctccgc tcttaggacg gggatcaaga gaggtcaaac ccaaaagaga tcgcgtggaa
    24301 gccctgcctg gggttgaagc gttaaaactt aatcaggcta gtttgttagt ggcgtgtccg
    24361 tccgcagctg gcaagcgaat gtaaagactg actaagcatg tagtaccgag gatgtaggaa
    24421 tttcggacgc gggttcaact cccgccagct ccaccaaaat tctccatcgg tgattaccag
    24481 agtcatccga tgaagtccta agagcccgca cggcgcaagc cctgcgggct tttttgtgcc
    24541 ctcaatttgt cccgcgaagt ccgaagaaaa ctaattaaat ccgatccttt taggcacctt
    24601 gttaggcacc tcataaagct ttattgtttt ttgaggtgcc taaaactatg gaaacccggc
    24661 aatggcaaga caaaccaaac ctctatccgt taaagaaata gaatcagcca aacccaagga
    24721 ggcggactac gttctctatg atggcgatgg ccttgagcta ctcattaaat ccagcgggag
    24781 taaaatctgg cagtttcgct acattcgccc tgtcaccaag aaacgagcaa agaagagcat
    24841 aggctcctac ccgtcagtta cccttgccga tgccagaaac taccgtgcag agtcccgctc
    24901 actactggct aaacagatcg atccgcagga acatcagcaa gaacaattgc gcagttcgct
    24961 ggaagccaaa accaacactt tccaactcgt agctgaacgt tggtggaatg tgaagaaagc
    25021 cagtgtgaca gaggactatg cggaagatat ctggcgctct cttgaaagag acgtctttcc
    25081 tgcgattggc gacgttagcg ttacagatat taaagctcat acactggttc aggccgtaca
    25141 accggttcag gccagaggag cactggaaac tgttcgtcgc ctgtgccaac gtattaatga
    25201 ggtcatgatc tatgcccaga acacagggct gattgatgct gttcccagcg ttaatatcgg
    25261 taaagccttc gagaagcctc agaaaaagaa catgcccagc attcgaccgg atcagctacc
    25321 tcagttaatg cagacgatgc gaacagccag cattagcctt tccacacgct gcctgttcat
    25381 gtggcaactt cttactatta cccgccctgc cgaagcggct gaagctctct gggaagaggt
    25441 agacatagaa gcgcgagagt ggaagattcc tgcagcacgc atgaaaatga accgcgacca
    25501 tactgttcca ttgtcagatg aagcaattgc gatactagag atgatgaagc cgttaagtgg
    25561 aaatcgagaa tttatctttc ccagccgcat caagccaaac cagccgatga acagtcagac
    25621 cgtaaacgca tcgctaaaac gcgcaggttt tggtggggtg ctcgtttcac acggcctgcg
    25681 atctatcgcc agtacagccc ttaatgagca gggatttcca cctgatgtta ttgaggctgc
    25741 acttgctcat gtggataaga atgaggttcg tcgtgcgtat aaccgtagtg attatcttga
    25801 gcagcgacgg cctatgatgc agtggtgggc tgattttgtg atggctgcag atcacggtag
    25861 tatgattgaa ggagggataa aggcaatgcg gttagttgga tgacatgtcg tagctaaaac
    25921 ccgttctgtt tttgggtaga ttaacctgaa ggtctgcaat tttaaatgac gctgaaagaa
    25981 gttaaaccct gctcttaagc agggttttta tgatttattc taattttggt tttgaatctt
    26041 tctattttaa atcagccttt gcggctcgaa tatctgcgtt ttttgctgag tattcccatt
    26101 cgtcagcgat tctaaatagc attgcagaag tacggacgca gtggggcatt gaatttgccc
    26161 attctcggta ctgctcagct aatatccttt cttggtttcc accttcgtaa cgcatacgag
    26221 caaatacccc ccttttattg tacctaccaa tttgta
//
LOCUS       DAFAVF010000052        26060 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFAVF010000052 DAFAVF010000000
VERSION     DAFAVF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.052"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="LGK42_002136"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="LGK42_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBK2658381.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="LGK42_002137"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="LGK42_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBK2658382.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="LGK42_002138"
     CDS             complement(941..1408)
                     /locus_tag="LGK42_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK2658383.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="LGK42_002139"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="LGK42_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK2658384.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="LGK42_002140"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="LGK42_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK2658385.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="LGK42_002141"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="LGK42_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK2658386.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="LGK42_002142"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="LGK42_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK2658387.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="LGK42_002143"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="LGK42_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK2658388.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="LGK42_002144"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="LGK42_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK2658389.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="LGK42_002145"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="LGK42_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK2658390.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="LGK42_002146"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="LGK42_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK2658391.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="LGK42_002147"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="LGK42_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK2658392.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="LGK42_002148"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="LGK42_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK2658393.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="LGK42_002149"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="LGK42_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK2658394.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="LGK42_002150"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="LGK42_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK2658395.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="LGK42_002151"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="LGK42_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK2658396.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="LGK42_002152"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="LGK42_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK2658397.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="LGK42_002153"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="LGK42_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK2658398.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="LGK42_002154"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="LGK42_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK2658399.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="LGK42_002155"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="LGK42_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK2658400.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="LGK42_002156"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="LGK42_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK2658401.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="LGK42_002157"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="LGK42_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK2658402.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="LGK42_002158"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="LGK42_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK2658403.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="LGK42_002159"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="LGK42_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK2658404.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="LGK42_002160"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="LGK42_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK2658405.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="LGK42_002161"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="LGK42_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK2658406.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="LGK42_002162"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="LGK42_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK2658407.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="LGK42_002163"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="LGK42_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK2658408.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="LGK42_002164"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="LGK42_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK2658409.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="LGK42_002165"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="LGK42_002165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK2658410.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="LGK42_002166"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="LGK42_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK2658411.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="LGK42_002167"
     CDS             15548..15916
                     /locus_tag="LGK42_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBK2658412.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNGGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="LGK42_002168"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="LGK42_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBK2658413.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="LGK42_002169"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="LGK42_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBK2658414.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="LGK42_002170"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="LGK42_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK2658415.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="LGK42_002171"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="LGK42_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBK2658416.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="LGK42_002172"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="LGK42_002172"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK2658417.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="LGK42_002173"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="LGK42_002173"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK2658418.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="LGK42_002174"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="LGK42_002174"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK2658419.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="LGK42_002175"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="LGK42_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK2658420.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="LGK42_002176"
     CDS             22593..23066
                     /locus_tag="LGK42_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBK2658421.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="LGK42_002177"
     CDS             23095..23637
                     /locus_tag="LGK42_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBK2658422.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="LGK42_002178"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="LGK42_002178"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBK2658423.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="LGK42_002179"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="LGK42_002179"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK2658424.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="LGK42_002180"
     CDS             25034..25291
                     /locus_tag="LGK42_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBK2658425.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="LGK42_002181"
     CDS             complement(25267..25821)
                     /locus_tag="LGK42_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK2658426.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRTLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6685 a         6182 c         6763 g         6430 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa tgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggtaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgactt ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatggcggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actggcagga ttacactcgc
    16381 gccgtaaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaaa
    16501 aataagaagg ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggtg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ttatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgtgc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag
//
LOCUS       DAFAVF010000053        26054 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFAVF010000053 DAFAVF010000000
VERSION     DAFAVF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.053"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(403..783)
                     /locus_tag="LGK42_002182"
     CDS             complement(403..783)
                     /locus_tag="LGK42_002182"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK2658427.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(783..1514)
                     /gene="pdxJ"
                     /locus_tag="LGK42_002183"
     CDS             complement(783..1514)
                     /gene="pdxJ"
                     /locus_tag="LGK42_002183"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBK2658428.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1526..2254)
                     /gene="recO"
                     /locus_tag="LGK42_002184"
     CDS             complement(1526..2254)
                     /gene="recO"
                     /locus_tag="LGK42_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK2658429.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2266..3171)
                     /gene="era"
                     /locus_tag="LGK42_002185"
     CDS             complement(2266..3171)
                     /gene="era"
                     /locus_tag="LGK42_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK2658430.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3168..3848)
                     /gene="rnc"
                     /locus_tag="LGK42_002186"
     CDS             complement(3168..3848)
                     /gene="rnc"
                     /locus_tag="LGK42_002186"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK2658431.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4121..5095)
                     /gene="lepB"
                     /locus_tag="LGK42_002187"
     CDS             complement(4121..5095)
                     /gene="lepB"
                     /locus_tag="LGK42_002187"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK2658432.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5111..6910)
                     /gene="lepA"
                     /locus_tag="LGK42_002188"
     CDS             complement(5111..6910)
                     /gene="lepA"
                     /locus_tag="LGK42_002188"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK2658433.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7108..7587)
                     /gene="rseC"
                     /locus_tag="LGK42_002189"
     CDS             complement(7108..7587)
                     /gene="rseC"
                     /locus_tag="LGK42_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBK2658434.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7584..8540)
                     /gene="rseB"
                     /locus_tag="LGK42_002190"
     CDS             complement(7584..8540)
                     /gene="rseB"
                     /locus_tag="LGK42_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBK2658435.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8540..9190)
                     /gene="rseA"
                     /locus_tag="LGK42_002191"
     CDS             complement(8540..9190)
                     /gene="rseA"
                     /locus_tag="LGK42_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBK2658436.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9223..9798)
                     /gene="rpoE"
                     /locus_tag="LGK42_002192"
     CDS             complement(9223..9798)
                     /gene="rpoE"
                     /locus_tag="LGK42_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBK2658437.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9795..9950)
                     /gene="rseD"
                     /locus_tag="LGK42_002193"
     CDS             complement(9795..9950)
                     /gene="rseD"
                     /locus_tag="LGK42_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBK2658438.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10206..11828
                     /gene="nadB"
                     /locus_tag="LGK42_002194"
     CDS             10206..11828
                     /gene="nadB"
                     /locus_tag="LGK42_002194"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBK2658439.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLELNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11813..12550)
                     /gene="trmN"
                     /locus_tag="LGK42_002195"
     CDS             complement(11813..12550)
                     /gene="trmN"
                     /locus_tag="LGK42_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBK2658440.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12682..14016
                     /gene="srmB"
                     /locus_tag="LGK42_002196"
     CDS             12682..14016
                     /gene="srmB"
                     /locus_tag="LGK42_002196"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBK2658441.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14225..15106)
                     /locus_tag="LGK42_002197"
     CDS             complement(14225..15106)
                     /locus_tag="LGK42_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2658442.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15209..15796
                     /gene="eamB"
                     /locus_tag="LGK42_002198"
     CDS             15209..15796
                     /gene="eamB"
                     /locus_tag="LGK42_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBK2658443.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15851..16234)
                     /gene="grcA"
                     /locus_tag="LGK42_002199"
     CDS             complement(15851..16234)
                     /gene="grcA"
                     /locus_tag="LGK42_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBK2658444.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16539..17228
                     /gene="ung"
                     /locus_tag="LGK42_002200"
     CDS             16539..17228
                     /gene="ung"
                     /locus_tag="LGK42_002200"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK2658445.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17276..18313)
                     /locus_tag="LGK42_002201"
     CDS             complement(17276..18313)
                     /locus_tag="LGK42_002201"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBK2658446.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSYIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18520..18939
                     /gene="trxC"
                     /locus_tag="LGK42_002202"
     CDS             18520..18939
                     /gene="trxC"
                     /locus_tag="LGK42_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBK2658447.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19008..19706
                     /gene="tapT"
                     /locus_tag="LGK42_002203"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19008..19706
                     /gene="tapT"
                     /locus_tag="LGK42_002203"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBK2658448.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19738..22398
                     /gene="pat"
                     /locus_tag="LGK42_002204"
     CDS             19738..22398
                     /gene="pat"
                     /locus_tag="LGK42_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBK2658449.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22512..23867
                     /gene="pssA"
                     /locus_tag="LGK42_002205"
     CDS             22512..23867
                     /gene="pssA"
                     /locus_tag="LGK42_002205"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBK2658450.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23913..24236
                     /locus_tag="LGK42_002206"
     CDS             23913..24236
                     /locus_tag="LGK42_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBK2658451.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24233..25531)
                     /gene="kgtP"
                     /locus_tag="LGK42_002207"
     CDS             complement(24233..25531)
                     /gene="kgtP"
                     /locus_tag="LGK42_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBK2658452.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRHRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25857..25972)
                     /gene="rrf"
                     /locus_tag="LGK42_002208"
     rRNA            complement(25857..25972)
                     /gene="rrf"
                     /locus_tag="LGK42_002208"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6264 a         6905 c         6522 g         6363 t
ORIGIN      
        1 aggaatgcag ctgttcagat tagcccttac atctccccaa aactgaacgt gcgagttatt
       61 gagggtgcat gctgcactcc acaccagagc tttgacgaca ccactcgttt caatggggga
      121 attctgtggc atggtgtaaa gcacagcaaa atcttcaata acgaagccaa ttttaatgta
      181 cttacgaatt ggcggtcacg ttaatctttc catcaatatt gctttcttcg taaaggctcg
      241 agtttttatg ctaaagattg caagttgctt gtaaaagata agtacactga tccataatcg
      301 ctgttgttga gggtgcatgc tgcacaaaat taaagttaaa aagtaaaacc cccgttcctt
      361 accagttcgg gggttttact ttttaaagag aacggtatta ttttaacttt caataattac
      421 cgtggcacaa gcatagtggc gttcatctgc cagcgttaca tgcatattta caacgccgag
      481 cttttccgcc agttttaatg cctcacccca tagccgtagc cgtggtttgc cgagctcatc
      541 attgaatact tcaaattgat taaacgccag accattgcgg atcccggtgc caaacgcttt
      601 tgctgcggct tctttcacag caaaacgctt cgccagaaaa cgcaccggct ggtggtgcgt
      661 tttccagatt gcccattcgt tatcgcttaa tacgcggcgt gccaggcgct caccggatcg
      721 ggcgatcact gcttctatgc gagcgatctc tacgatatcc gtgcctaaac ctaatattgc
      781 cattagccac gcgcttccag catcagacgc ttcatttctg ccaccgcatc tttcagtccg
      841 gtcatcactg cacgaccaat aatggcatga ccgatattca gttcatgcat ctcagggatg
      901 gcggcaatag ctttcacgtt gtgataggtc agaccgtgac cagcgttaac tttcagaccg
      961 agactagcgg caaaggtcgc ggctttggcg atacgcgcca gctcttgcgc ctgttcggcg
     1021 tcagttttgg catcagcata gcaaccggtg tggatctcga taaacggtgc gccaacctct
     1081 gccgcagctt tgatctgctc ttcatcggcg tcgataaaga gagaaacctg gatcccggca
     1141 tctgccagac gtttgcaggc atcgcgcatt ttgtcacgct gccctgcgac gtccaggccg
     1201 ccttcggttg ttacttcctg acgcttttcc ggtaccagac agcaaaaatg tggcttcgtc
     1261 tcaacggcga tcgccagcat ctcttcggtc accgccatct ccagattcat gcgggtatcc
     1321 agtgtctgac gcaggatgcg cacatcacgg tcagtaatgt ggcggcgatc ttcacgtaaa
     1381 tgcacggtaa tgccgtccgc tcccgcctgc tcagcaataa acgccgcctg caccggatcc
     1441 gggtaagcag taccgcgtgc gttacgcagc gttgcaatat ggtcaatgtt gacgcctaac
     1501 agtaattcag ccatgacaat cctcatcatt cataatgtgt tttcaccgtt cgcttaggca
     1561 taaactgccg gaacagttcc ctgctcttta atggtttacc gccaagatac ggcttaagcg
     1621 ccatgcgggt aaagcgtttc gcggcgcgta gtgtgtctgc gtcaggaaat tcccgcgcgt
     1681 ttaacgcttt taactgcctt ccggtgaacg ttttattgtc gataacgacg cttgcgataa
     1741 accctttttc ttcgcgataa cgatacgtca tggtgtcatc taccggctcg ccgctacccg
     1801 cacaatgggt aaaattgacg ccatagccca gatgcccgag cagtgccagt tcaaagcggc
     1861 gcagcgcggg ttctggcgta ccagtggcac ctgcaagaga ctgaatgcag tgcaagtaat
     1921 cgaaaaagag ttcagagaag cgcgtctcgt attccagtac gcgggagaga agttcgttga
     1981 tgtacagacc gctgtaaagc gtgataccgc ttaatggcag cgccagcgag acagcttcag
     2041 cactgcgcag cgttttgact tcgccacgcc cgccgaaacg tagcaagaga ggggtgaaag
     2101 gctgtaatgc acctttcaga gtagagcgtt tagagcgtgc gcctttggca accagacgca
     2161 cgcgacccga ttcctccgtg aagacgtcca gcatcaggct ggtttcgctc cacgggcgac
     2221 tatgcaggac aaatgcgcgc tgccagcctt ccatcggagt gactcttaaa gatcgtcaac
     2281 gtaaccgaga ctgcgcagtg cgcgttcgtc gtcggcccaa ccggatttca cttttaccca
     2341 cagttcaagg tggacaggcg cttcgaacat ttcctgcatg tctttacgcg cttcaatccc
     2401 gatggttttg atcttggccc ctttgttgcc aataaccatc ttcttctgcc cttcacgctc
     2461 aacgagaatc aaaccattga tgtcataacc accgcgttcg ttagagacga aacgttcgat
     2521 ctccacggtc acggagtacg gcagttcagc gccgaggaaa cgcatcagtt tttcgcggat
     2581 gatttcagac gccataaaac gctgtgagcg atcggtgatg taatcttccg ggaagtgatg
     2641 ggtcgcttca gggaggtgct tacgcacgat tgctgcgata gtgtcgacat tcagcccggt
     2701 ttcagcagag atcggcacga tatcgaggaa gttcatctga cttgccagga actgtaagtg
     2761 cggcagcaga tcggcttttt cctgcacgtt atccactttg ttcaccgcga ggatcaccgg
     2821 cgctttgcca tcgcgcagtt tgttgagcac catttcgtcg tccggcgtcc agcgggtgcc
     2881 ttcaacgaca aaaatcacca gctcaacgtc gccaatagag ctgctcgccg ctttgttcat
     2941 caggcggtta atagcgcgtt tttcttccat atgcaggcca ggggtatcaa cgtagatcgc
     3001 ctgatacgcg ccttcagtat ggatccccac aatgcggtga cgagttgttt gcgctttgcg
     3061 ggaggtgatg gagatcttct gccccagcag tttgttcaac aatgtggatt tgccaacgtt
     3121 cggacgtccg acgatggcaa taaatccgca gtaactttta tcgatgctca ttccagctcc
     3181 agttttttca acgcctgttc ggcggcagcc tgctcagcct tacgacggct tgaacctgtg
     3241 ccaaccaccg gttcactcag gccgctgacc tggcagtgga tagtaaattc ctgatcgtgc
     3301 gcttcgccac gtacctggac taccagataa gtcggcagcg gcagatggcg accctgcaaa
     3361 tattcttgca agcgcgtttt cggatctttt tgtttatcgc ctgggctaat ttcgtccaaa
     3421 cgagtttgat accagttgag gattaatttc tcgacggttt gaatatcact gtcgaggaat
     3481 acgccaccaa ttaatgcttc gacggtgtcg gcgagaattg actcacgacg aaatccaccg
     3541 cttttaagtt cacctggccc taaacgtaag cactcgccta actcaaattc gcgcgccagt
     3601 tccgccagcg tattgccacg gaccagcgtg gcgcgcatcc ggctcatatc gccttcatcc
     3661 acacgaggga aacggtgata aagcgcattg gcgataacgt agctcagaat agagtcgcct
     3721 aaaaattcta aacgctcgtt atgtttactg ctggcactac gatgagttaa tgcctgctgc
     3781 aacagttcct gatgattaaa agtgtagccc agcttccgtt gaagccgatt aattacgatg
     3841 gggttcatgc gataccaata aataaatgcg tcaacaattc agcacacgaa acagacctga
     3901 tatacatggc tctgctaact gcttcgctgc agtttcagtt taccggtata tggggaccaa
     3961 cgctgtttcg tgtgccgtgg caacctggag gtgccaacct taaacttcgg gggaatattc
     4021 tatacacaac gacgggggat gtcgttagcc acgggagatt tatctcataa ataattcacg
     4081 ttgtcgccat aacggcgaca acgtgaacga agatgagcta ttaatggatg ccgccaatgc
     4141 gacttaagcg cacaccagtc ggccattcgc cttcttgctt atcgaagctc atccagatag
     4201 ccgttgcccg accgaccaga ttcgcttcag gcacaaaccc ccaataacgg ctgtccgcgc
     4261 tgttgtcgcg gttgtcgccc atcatgaagt attgtcccgg aggaacaatc caggttgcca
     4321 gttgttgccc tggctgctgg taatacatcc ccacctgatc ctgcgcaatc ggcactgtca
     4381 gaattcggtg cgtcacatca cccagtgtct ctttacgctc ggaaagacga attccatttt
     4441 ctttggtttc gtttttcggc acttcaaaga atccgctggt cgcttcccca ccattacggc
     4501 gtgagaaggt ctgaacgaaa tcgctcggtt ccacgtttga gtaggtgacc ggcagcgcgt
     4561 tttcacacgc ctggccggaa ctgcatcccg gttgaatcgt cagctctttc gagaccggat
     4621 cgtaagtgac tttatcgccc ggtaaaccca ccgcgcgctt gatgtaatca agctttggat
     4681 cttccggata tttaaagacc acgatatctc cgcgtttcgg atgaccggtt tcgatcagcg
     4741 ttttctggta gataggatct ttaatgccat aagcaaactt ttctaccaga ataaaatcac
     4801 cgattaacag agtcggcatc atcgaacctg acgggatctg gaacggttca taaataaacg
     4861 aacgcacaat caatacgata gccagcaccg gaaaaacaga agcaccggtt tccagccagc
     4921 caggcttcgg cgcaaccttt ttcagggttg ctttatccag tgagtcaccg gcagccgcct
     4981 gcgccgctgc ctgacgttcc cgccgtttag gtgcgaaaaa gaatttatcc acgcaccata
     5041 aaatgcccgt caccagtgtg gcaatcacca gaatcagggc aaacatattc gccatgccaa
     5101 ctcctaaggg ttatttgttg tctttgccga cgtgcagaat ggcgaggaac gcttcctgcg
     5161 gcagctcgac gttaccgatc tgcttcatgc gtttcttacc ttctttctgc ttctgcagca
     5221 gctttttctt acggctgata tcgccgccat aacatttagc cagtacgttt ttacgcagct
     5281 gtttcacggt ggatcgcgca ataatgtgcg taccaatcgc tgcctgaatg gcgatatcaa
     5341 actgctggcg tgggatcaga tctttcatct tctccaccaa ctcgcgaccg cggttttgcg
     5401 aattatcacg gtgggtgatc agcgccaacg catcaacacg ttcaccgttg attaatacgt
     5461 ctacacgtac catgtcggac gcctggaagc gtttgaagtt gtaatccaga gacgcataac
     5521 cacgcgaggt agatttcagg cgatcgaaga agtcgagcac cacttccgcc atcgggatct
     5581 cgtacgtcag cgccacctga ttaccgtggt aaaccatatt ggtctgcacg ccgcgttttt
     5641 ctacgcacag cgtaataacg ttgccgagat atgcctgcgg cagcagcatg tgacattcgg
     5701 caatcggctc gcgcagttcg tagatgttat ttaccgctgg cagcttggat ggactgtcga
     5761 cgtaaataac ttctcttgac gtggtttcaa cttcatacac tacggtcggc gcagtggtga
     5821 tcagatccag atcgtattca cgttccagac gttcctggat gatctccatg tgcagcaggc
     5881 cgaggaagcc gcagcggaaa ccaaagccca gcgcgctgga gctttccggc tcatagaaca
     5941 gtgaagcatc gttcaggctg agtttaccca gcgcgtcacg gaaggcttca tagtcgtcgg
     6001 aacttaccgg gaacagaccg gcgtatacct gcggtttgac tttcttaaag ccaggcagcg
     6061 ccttttctgc cggattacgc gccagcgtta aggtatcgcc gactggagcg ccgtggatat
     6121 ctttaatcgc acataccagc cagcctactt cgccacattt cagttcagtg cggtcaacct
     6181 gtttcggcgt gaagatgccc agacggtcgg cgttataggt ctgcccggta ctcatgactt
     6241 tcactttgtc gcccttacgc agggtgccgt ttttaatacg gataagtgaa acaacgccca
     6301 ggtagttgtc gaaccatgag tcgataatta gtgcctgcaa cgggccttcc ggatcgcctt
     6361 ccggcggcgg aatgtcgcgc accagacgtt cgagaacgtc ctgcacgcca acgccggttt
     6421 tcgctgaaca gcgcaccgcg tcggtggcgt cgatgccgac gatatcttca atttcttccg
     6481 ccacgcgttc aggatcggct gccggcaggt caatcttgtt cagtaccggc accacttcga
     6541 gatccatttc catggcggtg tagcagtttg ccagggtttg cgcttctacg ccctgcccgg
     6601 cgtcgaccac cagcaatgca ccttcacagg cagccagcga acgggaaact tcataggaga
     6661 agtcaacgtg gcccggggtg tcgataaagt tcagctgata ggtttcgccg tcagacgctt
     6721 tgtagtccag cgtcacgctt tgcgctttga tggttatgcc acgctcacgc tcaagatcca
     6781 tggaatcgag aacctgcgcc tccatttcac ggtcagacag gccaccgcag atctggataa
     6841 tacggtcaga cagcgtcgat ttaccgtggt caatgtgagc tatgatcgaa aagttacgta
     6901 tattcttcat aaagtatgat tattgtgcct taatgcccgg ttaaccaggc ttttagaagt
     6961 cgctgttctg agcttaacgt ctgtattaat agaaacgccg cattctacac tacaacattg
     7021 aggcgaggaa atgttcatac cgtatggatt gtggtatctg gaaacgtcct cgcatttgtt
     7081 atgcaaaatg caacaaagcc agtgaaatca ctggctcgcg tcttccgaag atgtttcaaa
     7141 tcgcacaagg ccaggcggca atgccacgct taagatgatc ggttgccatt ctgcccgggc
     7201 agcaaactta cgcgagtagc cgcgcgcaat caggaacccg ccaataccgc cgaggaccgc
     7261 accacataat gctgcgacgt cagaagcaaa gagtagctga aatagcgaag cgatgaggaa
     7321 taatcccacc agcggcgaca tataaaccag taatgcggag ctaagcaggc tgccttcggc
     7381 gatccccaat tccacttttt gccccggcac taacggctca tcacagggta cgacaatggt
     7441 atgcgtggtt tgcgggccaa gtttatttaa cacgcggcta ccgcaaccgg cgcgtgaagc
     7501 gcagctgctg catgaagctt taacatcaca actgaccagc gcctgcccgt tttgccagga
     7561 gacgacggta gcccactctt tgatcattgc gctgccccga acttaatatt ctcggcaatg
     7621 cgtttcgccg tttgcggcgg cagttcaccg acaatggtga tttcggcgtt atcacgtacg
     7681 cttgtactga cggttctgcg tccggtgcgc aacatctgat cggtgctcga tggcgtagcg
     7741 cggttaacgt ttaccgagaa gctgaataat ccgtcggaat agagacgtga ttcgataggc
     7801 atgttgtcca tcgtcggtag cggacgtcga ctactggaaa cttcgctaaa accctgtggc
     7861 aaccaggttg gcgtccagct gaatttagct ttttcaccta caggaacaga aagcaacggc
     7921 ggcaaatttg ccttcgccag cgtctgcata ctgctgctga tatcctgatt gacgttaaaa
     7981 gcaatcacgc gaaattgttc cagcgtttca ccatcgcgat caaggagatc aacccgcatc
     8041 ggtaatttcg attcggtgtc catccacacg atgtagctgt agcgtgtacc atctcgggca
     8101 accacgcgaa tgacttcgca aagacgatca gcaatacgcg tgcgcccgac ggagataaag
     8161 tcgtagtaag gagaaaggcg tttgaaatcg gtatagataa gcgatggcag agaatcaaca
     8221 atgtaatcgc cattaagcgt gaacggttca agtcccggtt caaaatagct gatttcattg
     8281 ccgcgctgta ccacttcccg gcgcgggcca tccatttgca acaattgtgc aagagggcgg
     8341 ttatcgaggc gtgcatgtcg ataacgcaga gactcaacac cctgtttatt gatgctgatg
     8401 aatgacagct cgtaattcag tgactgactg gccaggttca tctgctgtaa taacgccccg
     8461 gacgcgggag tggccgaggc gttagcagag aataacaggc tacctgtcac taatgacatg
     8521 gcaaaccaaa gttgcttcat tactgcgatt gcgttcctaa agtttgaatt cctggcacct
     8581 gtacagcggc ttgctgggtt tgcgcctgct caaactgaag ctgttcagag tggagtcggc
     8641 gttgcagttc gtaatcctgc aacattgcat taatgcgacg acgctgctcc tgtacctgct
     8701 gctgttgacc attgtttgcg gtcgcttcag aaggtactcc caggcttacc gggctggctt
     8761 tacccatcat cggcagtgta ttaaataccg gcgtttcggg ctgctgggac gtttcagatt
     8821 gtccattata gtgctggacg ccaacgataa ctgcaagcga tacgcatgct gctacgccca
     8881 tttgggtaag ctgtgccgcc cacggacgta ctttctgcca gaatggcatt ttctgccatt
     8941 gatgcggcgc aggctgggct tccgggatca atgtcgccgg ttgacgtact ggctcttctt
     9001 caatggcggc catcacgcgt gaagagatat cgaaatggag cacctcggga gtatcacccc
     9061 gcattgagtc acggattaag tgatagcttt cccaggtttt ctgcatttct gggttatgag
     9121 ccagttcgtt aagcagctca ctatccagcg tttcgccatc cattaaagcg gaaagttgtt
     9181 ctttctgcat gcctaatacc cttatccagt atcccgctat cgtcaacgcc tgataagcgg
     9241 ttgaactttg ttatcaatag cttccctcgc tcggaagata cgtgaacgca ccgtacctac
     9301 cggacaatcc atgatagcgg ctatctcttc atagctcagg ccatccagct cccgcaaggt
     9361 tattgccatg cgcaaatctt ccgggaggga ctcaatagtt cggaaaacta tctgtctcag
     9421 ttcttctgac aacattaagt tctcagggtt cgaaatttct ttcaacgcgc cgccactttc
     9481 gaagttttca gcttcaatgg catccacatc actggaaggt ggacgacgcc cctgagcaac
     9541 caggtaattt ttcgctgtat ttacagcaat ccgatacagc catgtataaa aagcgctatc
     9601 tccccggaac gaatccagcg cacgataggc tttaataaaa gcttcttgta ccacatcggg
     9661 aacatcaccc gacggcacat agcgggaaac cagactcgcc actttatgct gatagcgcac
     9721 taccagtagg ttaaaggctt tctgatctcc cttctggacc cgttcaacca ggacctggtc
     9781 cgttaactgc tcgctcatcc gaggtaaagt ctccccaaac caaatttcca cgcgctatcg
     9841 aaacgccact ccattagctg caatttgagc aagcaaaggg ttagagtgtc tcgtttttgt
     9901 aaagttccgt aacgcatctg tttttgtttg tcatgctgta gacggatcat tatctatcat
     9961 tataagtcta cagaatctga acatcgcatt atctgtgtag aaatgcccat ttaactgcct
    10021 gaagagtaac ccaacggcct ttttatttca ccacctaatc ctccaccagc cagtaacttc
    10081 tctttttctc gccgccctgc gtcagcgtgt ttagccactg taacaaatat taaaatagca
    10141 ggtgtttatt cgcacaacat gatgctatgc tgaccaaacg gtgtttagta aattaaacaa
    10201 agaaaatgaa tactctccca gaacattcat gtgacgtgtt gattatcggt agcggcgcag
    10261 ccggactttc actggcgcta cgcctggctg accagcatca ggtcatcgtt ctaagtaaag
    10321 gcccggtaac ggaaggttca acattttatg cccagggcgg tattgccgcc gtgtttgatg
    10381 aaactgacag cattgactcg catgtggaag acacattgat tgccggggct ggtatttgcg
    10441 atcgccatgc agttgaattt gtcgccagca atgcacgatc ctgtgtgcaa tggttaattg
    10501 accaaggggt tttgtttgat acccacgttc aaccgaatgg cgaagaaagc taccatctga
    10561 cccgtgaagg tggacatagt caccgtcgta ttcttcatgc cgccgacgcc accggtagag
    10621 aagtacaatc cacgctggtg agcaaggcgc agaaccatcc gaatattcgt gtgctggagc
    10681 gcagcaacgc agttgatctg attgtttctg acaaaattgg cctcccgggc acgcgacggg
    10741 ttgttggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
    10801 cagtggtact ggcaaccggc ggtgcgtcga aggtttatca gtacaccacc aatccggata
    10861 tttcttctgg cgatggcatt gctatggcgt ggcgcgcagg ctgtcgggtt gccaatctcg
    10921 aattgaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
    10981 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
    11041 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
    11101 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
    11161 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
    11221 tcacacaaga accggtaccg attgtgcctg ctgcacatta tacctgcggt ggtgtaatgg
    11281 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
    11341 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
    11401 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
    11461 taccgccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
    11521 actggcacga gctacggctg tttatgtggg attacgttgg cattgtgcgc acaacgaagc
    11581 gcctggaacg cgccctgcgg cggataacca tgctccaaca agaaatagac gaatattacg
    11641 cccatttccg cgtctcaaat aatttgctgg agctgcgtaa tctggtacag gttgccgagt
    11701 tgattgttcg ctgcgcgatg atgcgcaaag aaagccgggg gctgcatttt acgctggatt
    11761 acccggaact gctcacgcat tccggtccat cgatcctttc ccccggcaat cattacataa
    11821 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
    11881 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
    11941 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
    12001 tgcttaatgc cagttcagta aaaccattac caatctgctc tggcagcacc acgcagaaaa
    12061 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
    12121 tggtatagcg agcctgttcc cgttgaggtg tcgcgcactc tactccctgc tgatagtaag
    12181 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgatccac tgctgaatat
    12241 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
    12301 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca ctggttcgct
    12361 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
    12421 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
    12481 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
    12541 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
    12601 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
    12661 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
    12721 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
    12781 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
    12841 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
    12901 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
    12961 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat caccggcggc
    13021 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
    13081 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
    13141 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
    13201 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
    13261 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
    13321 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
    13381 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
    13441 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
    13501 aacaactgct atctcgaagg tgagatggta caaggcaagc gtaacgaagc gatcaagcgt
    13561 ttgaccgaag gtcgcgtaaa cgtactggtt gcaaccgatg ttgccgcgcg cggtatcgac
    13621 attcctgacg tcagccacgt ctttaacttc gatatgccgc gcagtggcga tacttatttg
    13681 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcaatttc gctggtggaa
    13741 gcccatgacc atttgctgct gggcaaggtt ggccgttaca ttgaagagcc gattaaagct
    13801 cgcgttattg atgagttacg cccgaaaacg cgtgcgccaa gcgagaagca gaccggcaag
    13861 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaaa
    13921 ccgcgtgtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccaagc
    13981 ggaacgggcg tgccaccgca aacgacagaa gagtaatctc aatgccgggt ttaagcctgg
    14041 tattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    14101 gctcatgcaa tatattgaat tttcacgatc ttgtaggccg gataaggcgt tcacgccgca
    14161 tccggcatga ataaagcgca ctttgtcgac aagttaaatg ccgggtttaa tcccgacatt
    14221 atttttatcc agccacaaaa ctctcttcaa cacactgaat aaaagtgtgc atcgccgggc
    14281 taaccgcttt tccagcatgg tgtgcgcaca tggccgtaat ggtctgcgac tgttcgccaa
    14341 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
    14401 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
    14461 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
    14521 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
    14581 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
    14641 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
    14701 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
    14761 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
    14821 aaacaacgcg tagttccccg tccggatccg actctttttt cgcagcctca cgaagcgtat
    14881 ccatcactcg ggtaagttca tagatgtgcg gcagcaactt ttttccttcg cgggtaaggc
    14941 acattcgtcg accaattttc tcaaacaatt gtaccgagaa ctcctgctca agctgctgaa
    15001 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
    15061 cctcttccac cacggtttta agtgtaataa aacggcgcag atccataacc ccaaacctat
    15121 cgaaaatatc gaatttagaa tgtaaaaaca ttcatttttt taaatgttcc gtgtcgggta
    15181 ctgtctacca aaacagagga gataacaagt gacaccgacc cttttaagtg ctttttggac
    15241 ttacaccctg attaccgcca tgacgccagg accgaacaat attctcgccc ttagctctgc
    15301 tacgtcgcat ggatttcgtc aaagtacccg cgtgctggca gggatgagtc tgggattttt
    15361 gattgtgatg ttactgtgtg cgggcatttc attttcactg gcagtgattg acccggcagc
    15421 ggtacacctt ttgagttggg cgggggcggc atatattgtc tggctggcgt ggaaaatcgc
    15481 caccagccca acaaaggaag acggacttca ggcaaaacca atcagctttt gggccagctt
    15541 tgctttgcag tttgtgaacg tcaaaatcat tttgtacggt gttacggcac tgtcgacgtt
    15601 tgttctgccg caaacacagg cgctaagctg ggtagttggc gtcagcgttt tgctggcgat
    15661 gattgggacg tttggtaatg tgtgctgggc gctggcgggg catctgtttc agcgattgtt
    15721 tcgccagtat ggtcgccagt taaatatcgt gctcgcgctg ctgctgatct attgcgcggt
    15781 acgcattttc tattaattaa aaaagcggaa gaggtcgccc tcttccgctt agtaacttgc
    15841 tacttaagcc ttacaggctt tcagtaaagg tacgagcgat aacgtcgcgc tgctgttccg
    15901 gagtcagaga gttaaagcga actgcataac ctgatacacg gatggtcagc tgcggatatt
    15961 tttccggatg cttaactgca tcttccagag tttcacgacg cagaacgtta acgttcaggt
    16021 gttgaccacc ttcaacgcga acttctggtt tcacttctac tggaacttca cggtattcaa
    16081 tgtcacccag tttgcttact gcaaccactt catcttctgc ataacctgct tttgcaacga
    16141 tgcaacgcgc ttcgcctttt tcgctgtcca gcagccagaa agagttcagc agatcgtcgt
    16201 tagcggcttt agtaatctgg atacctgtaa tcatgtgatg cctccccggc aaaattattt
    16261 gatttattca gcctgtcgcg gccaattggt aaaaccattg ttgcttgagt gtatatatac
    16321 tcctcaacaa cccttgattc cttgatttaa atcaataaaa accacacatc gattgtggtc
    16381 gcaaatggat tttattgttt tacatcaact tatgcgggtg tgaaatttta ccaatttaca
    16441 tttttttgca ctcgtttaag tctaaaaaat gagcatgatt ttgttctgta gaaataagca
    16501 gttaagctag gcggattgaa gattcgcagg agagcgagat ggctaacgaa ttaacctggc
    16561 atgacgtgct ggctgaagag aagcagcaac cctattttct taataccctt cagaccgtcg
    16621 ccagcgagcg gcagtccggc gtcactatct atccaccaca aaaagatgtc tttaacgcgt
    16681 tccgctttac cgagttgggt gacgttaaag tggtgattct cggccaggat ccttatcacg
    16741 gaccgggaca ggcgcatggg ctggcatttt ccgttcgccc cggaatcgcc actcctccgt
    16801 cactgctgaa tatgtataaa gagctggaaa atactattcc gggcttcacc cgccctaatc
    16861 atggctatct cgaaagctgg gcgcgtcagg gcgttctgct gctcaatact gtgttgacgg
    16921 ttcgcgcagg tcaggcgcat tcccacgcca gcctcggttg ggagacgttt actgataaag
    16981 tcatcagcct gattaaccag caccgcgaag gcgtggtgtt tttgttgtgg ggatcacatg
    17041 ctcaaaagaa aggggcgatt atagataagc aacgccatca tgtactgaaa gcaccgcatc
    17101 cgtcgccgct ttcggcgcat cgtggattct ttggctgcaa ccattttgtg ctggcaaatc
    17161 agtggctgga acaacgtggc gagacgccga ttgactggat gccagtatta ccggcagaga
    17221 gtgagtaaat ttgcggggaa atgccggatg gcagagttgc cacccggctg atttatcagg
    17281 ctttattctg acgccaccat tcaccaagca aaacgccggt tgcgacagag atattcagcc
    17341 cggcaacgtt gcccgtaccg tcaatcttca cgcgcagatc gttcggatcg cgtgcggcat
    17401 ccggtaaccc ttcatattcc tgacccagca ccagtaccat tttcgctggc agactggttt
    17461 tgaacagcgg tttaccctgc tcgctggaag tggtcactac ggtgtaacct gcctgacgga
    17521 aatcatccag cacgttaaca atgttgtcgc cagtaatcgg ctgaacgtgc tctgcgccgc
    17581 cttctgcggt acggatagcc gcccccgact ccagcagtgc cgcatcctgc accacaacgc
    17641 ctttcacgcc gaagtgcgcg cagctgcgca tcatgccacc caggttatgc gggttagatt
    17701 cgttttccag cgccagtaca caatcctgcg cgcctgcctg gcttacccac tgctgcacgg
    17761 ttgtaccgtt acgctttttg atcaagaagc aaacgccgcc gtgatgttcc gtgcctgacg
    17821 cttttgtcaa ttccgcttca tccaccacat ggtacgcttt gcggtttgcc gccatccagc
    17881 gcaaggcttc tttaaaacgt ggcgttacgc tctggataaa ccaggcgcga acaatcgctt
    17941 ccggacggct ctggaacagt gcctgacatg cattttcgcc gtagacgcgg gtttcttccg
    18001 cacgctgacg acgcaaaact tctggatcaa tataactttt accactgatg ccaccgtgat
    18061 cggccttttc cggcgtctca tcacccggcg cgcgggaaac cgtgcgccac ggcgaatctt
    18121 cccacttgcg gtcacggggc tgactctgtt tgtcatcgcg ggcggggcga cggccaccgt
    18181 cagcacgaga ttttcctgga cgcccgcccc ctttcccggt acgcgggttg tgggtacgtt
    18241 tatcagaatc atcatcactg cggacataca tcactttgac cttgccgctt ttacctttca
    18301 tttcatcgtt catgcttttc tccaccagcg ctgcgcgaag cgcgcagatt acccgaagtc
    18361 cgcgcggttc gccatgattt cgtaccaaag cctgcgacta tcatacctat tgaataaaac
    18421 agattgttgt ctggaacaat gtccccgata atatgtaaca tattagaaac ataccggcgt
    18481 cgttgccgat aagtctcctt actcatcccg aggttagtta tgaataccgt ttgtacccat
    18541 tgtcaggcca tcaatcgcat tcccgacgat cggatcgaag atgcggcaaa atgcggacgc
    18601 tgcggtcacg acttgtttga cggagaggtg attaatgcga ccggtgaaac actcgacaaa
    18661 ttgctgaagg atgatctacc tgtggtgatc gacttctggg caccgtggtg cggcccctgc
    18721 cgtaatttcg caccaatttt tgaagatgtc gcgcaagagc gtagcggtaa agtgcgcttt
    18781 gtgaaagtga ataccgaagc tgaacgtgaa ttgagcagtc gctttggaat tcgtagtata
    18841 ccgacgatca tgattttcaa aaacggtcag gttgtcgaca tgcttaatgg cgcagtaccg
    18901 aaagcgccgt tcgacagctg gctgaacgaa tctctttaat cttaccgggg cgcatcttgt
    18961 gccccgtttt ctcctctgcg acaatggcgt tttttcgacg ctctcttatg accgaaaacg
    19021 ctgttctcca gttacgcgcc gagcgtattg cgcgcgcaac acgtcctttt cttgcccgcg
    19081 gtaatcgcgt tcgtcgctgc caacgctgtc ttttgccaga gaaattatgt ctctgttcga
    19141 caattacccc agcacaagca aaaagtcgct tctgtttgct gatgttcgac accgagccaa
    19201 tgaagcccag taataccggg cgtctgattg ctgatatttt gcctgatacc gttgcgtttc
    19261 aatggtcgcg taccgaaccc tcgcaggatt tgctggatct ggtacaaaac ccggactatc
    19321 aaccaatggt ggtctttccc gcctcgtatg ctgatgagca acgggaagtg atcttcacac
    19381 cacctgccgg taagccaccg ctgtttatca tgctcgatgg tacctggccg gaagctcgca
    19441 agatgtttcg taaaagtccg tatctggata atcttcccgt catttccgtc gatctttccc
    19501 ggctttctgc ctatcgcctg cgtgaagccc aggctgaagg ccaatattgt actgccgagg
    19561 tagccatcgc actgttagat atggccggcg ataccggggc ggcggcaggg ttaggcgagc
    19621 attttacccg ctttaaaaca cgctatctgg caggaaaaac gcaacatctg ggtagcatca
    19681 cagcagaaca gttagaaagc gtttaaaatc attcggtcac ttctgcggga gaccggtatg
    19741 agtcagcgag gactggaagc actactgcga ccaaaatcga ttgcggtaat tggcgcatcg
    19801 atgaaaccca atcgcgcagg ttacctgatg atgcgtaacc tgctggcggg aggctttaac
    19861 ggaccggtac tcccggtgac gccagcctgg aaagcggtgt tgggtgtgtt ggcctggccg
    19921 gatattgcca gcttgccctt tacacccgac cttgcggttt tatgtactaa tgccagccgt
    19981 aatcttgctc ttctggaaga gctcggcgag aaaggctgta aaacctgcat tattctttcc
    20041 gccccggcat cgcaacacga agatctccgc gcctgcgccc tgcgccataa catgcgcctg
    20101 ctaggaccaa acagtctggg tttactggct ccctggcaag gtctgaatgc cagcttttcg
    20161 cctgtgccga ttaaacgcgg caagctggcg tttatttcac aatcggctgc cgtctccaac
    20221 accatcctcg actgggcgca acagcgtgag atgggctttt cctactttat tgcgctcggc
    20281 gacagcctgg atatcgacgt tgatgaattg ctcgactatc ttgcgcgcga cagtaaaacc
    20341 agcgccatcc tgctctatct cgaacagtta agcgacgcgc gacgctttgt ttcggcggcc
    20401 cgtagtgcct cgcgtaataa accgattctg gtgattaaaa gcggacgtag cccggcggca
    20461 cagcgactgc tcaacacgac ggcaggaatg gatccggcgt gggatgcggc tattcagcgt
    20521 gccggtttgt tgcgggtaca ggacacccac gagctgtttt cggcggtgga aacccttagc
    20581 catatgcgcc cgctgcgtgg cgaccggttg atgattatca gcaacggtgc tgcgcctgcc
    20641 gcgctggcgc tggatgcctt atggtcacac aatggcaagc tggcaacgct aagcgaagaa
    20701 acctgccaga aactgcgcga tgcactgcct ggacatgtgg caatctctaa cccgctcgat
    20761 ctgcgcgatg acgccagcag cgagcattat gtcaaaacgc tggacattct gctccacagc
    20821 caggattttg atgcgctgat ggttattcat tcacccagcg cagcggctcc cgcaacagaa
    20881 agcgcgcaag aattaattga agcggtaaag catcatcccc gcagcaaata tgtctctttg
    20941 ctgacgaact ggtgcggcga gcactcctcg caagaggcac gacgtttatt cagcgaagcc
    21001 gggctgccga cctatcgcac gccggaaggc accatcaccg cgtttatgca tatggtggag
    21061 taccggcgta atcagaagca actacgcgaa acgccggcgt tgcccagcaa tctgacttcc
    21121 aataccgcag aagcgcatct tctgttgcaa caggcgattg ccgaaggggc tacgtcgctc
    21181 gatacccatg aagttcagcc tatcctgcaa gcgtatggca tgaacacgct ccccacctgg
    21241 attgccagcg atagcaccga agcggtgcat attgccgaac agattggtta tccggtggcg
    21301 ctgaaattgc gttcgccgga tattccacat aaatcggaag ttcagggcgt aatgctgtac
    21361 ctgcgtacag ccaatgaagt ccagcaagcg gcaaacgcta ttttcgatcg cgtaaaaatg
    21421 gcatggccgc aggcgcggat ccacggcctg ttggtgcaaa gtatggctaa ccgggctggc
    21481 gctcaggagt tgcgggttgt ggttgagcac gatccggttt tcgggccgtt gatcatgctg
    21541 ggtgagggag gtgtggagtg gcgtcctgaa gatcaagccg tcgtcgcact gccgccgctg
    21601 aacatgaacc tggcccgcta tctggttatt caggggatca aaagtaaaaa gattcgtgcg
    21661 cgcagtgcgc tacgcccatt ggatgttgca ggcttgagcc agcttctggt gcaggtttcc
    21721 aacctgattg tcgattgccc ggaaattcag cgtctggata ttcatccttt gctggcttct
    21781 ggcagtgaat ttaccgcgct ggatgtcacg ctggatatcg cgccgtttga aggcgataac
    21841 gagagtcggc tggcagtgcg cccttatccg catcagctgg aagaatgggt agaattgaaa
    21901 aacggtgaac gctgcttgtt ccgcccgatt ttgccagaag atgagccaca acttcagcaa
    21961 ttcatttcgc gagtcaccaa agaagatctt tattaccgct actttagcga gatcaacgaa
    22021 tttactcatg aagatttagc caacatgacg cagatcgact acgatcggga aatggcgttt
    22081 gtagcggtac gacgtattga tcaaacggaa gagatcctcg gcgtcacgcg tgcgatctcc
    22141 gaccctgata acatcgatgc cgaatttgct gtgctggttc gctcggatct caaagggtta
    22201 ggcttaggtc gacgcttaat ggaaaagttg attacctata cgcgagatca cggactacaa
    22261 cgtctgaatg gtattacgat gccaaacaat cgtggcatgg tggcgctagc ccgcaagctc
    22321 gggtttaacg ttgatatcca gctcgaagag gggatcgttg ggcttacgct aaatcttgcc
    22381 caacgcgagg aatcatgagt aaggtactgg aaatgttgac cacttaatcg ggactggtgt
    22441 tattattgcc cgcttatgtc gtctgcattg cacagaggac ccttcaatga acagagaaga
    22501 aatgcactgt gatgttgtca aaatttaagc gtaataaaca tcaacaacac cttgcccaac
    22561 tacccaagat ttctcaatca gttgatgatg tcgatttctt ttacgctccc gccgacttcc
    22621 gggagacgct gctggaaaaa atagccagcg cgaagcagcg catttgcatt gtcgccctgt
    22681 atctcgaaca ggatgacggt ggcaaaggca ttctgaacgc gttgtatgaa gctaaaaggc
    22741 agcgtccgga actggatgtg cgggtgctgg tcgactggca tcgtgcacaa cgtggacgca
    22801 ttggcgctgc ggcatctaac actaacgctg actggtactg ccgcatggcg caggaaaatc
    22861 cgggcgtaga tgttccggtt tatggcgttc caatcaatac ccgtgaagcc cttggtgttc
    22921 tgcactttaa aggctttatc atcgacgata gcgtacttta tagcggtgcc agcctgaacg
    22981 atgtttacct gcatcagcac gataaatatc gctacgaccg ttatcatctt atccgtaacc
    23041 gtaagatgtc agacattatg tttgaatggg ttacacagaa tattatgaat ggccgcggcg
    23101 ttaatcgtct ggatgatgtt aatcggccaa aaagcccgga aatcaagaac gatattcgtc
    23161 tgttccgcca ggagctgcgt gatgccgctt atcatttcca gggcgatgcc gacaacgatc
    23221 agctttccgt aacgccgcta gtggggctgg ggaaatcgag tctgttgaac aagaccattt
    23281 tccatcttat gccttgtgcc gagcagaaac taaccatctg tacgccatac ttcaacctgc
    23341 cagcaatcct tgtgcgcaat attatccagt tgctgcgcga agggaaaaag gtcgaaatta
    23401 ttgttggtga taaaaccgcg aacgacttct acattccgga agatgaacct ttcaagataa
    23461 ttggcgcatt gccttatctc tatgagatca atctgcgtcg tttcctgagc cgtttgcagt
    23521 attacgtcaa tactgaccag ctagtggttc ggttatggaa agatgacgac aacacctatc
    23581 acctaaaagg gatgtgggtt gatgataagt ggatgttgat caccggtaat aacctgaacc
    23641 cgcgcgcctg gcgtctggat ctggaaaacg ccattttgat ccacgatccg caacttgagc
    23701 tggcgccaca gcgagagaaa gaactggagc tgatccgcga gcataccacc atcgttaagc
    23761 actatcgcga tctgcaaagt attgccgatt atccggtgaa ggttcgtaaa ctcatccgcc
    23821 gtttgcgccg tatccgcatc gaccgattaa ttagccgcat cctgtaatca caaccccgtc
    23881 ctgtacgggg tttgtttttt ggaggccacg ttttgcgtat tctttttgtc tgttcgctat
    23941 tgttactttc tggatgcagc catatggcta acgatagctg gagcgggcag gataaagctc
    24001 aacactttat cgcctcggcg atgctttccg ccgccggaaa tgaatattca cagcatcagg
    24061 ggatgagccg ggatcgcagt gccatgtttg gattgatgtt ctctgtcagt ttgggggcgt
    24121 caaaagagct ttgggatagc cgccccgaag ggagcggctg gagctggaag gatttggcct
    24181 gggatgtcgc cggtgcaagc accggctata ccgtctggca actgacccgt cactaaagac
    24241 gcatcccctt ccctttgcga tgtagcatca aagaaaccag aaacgccacc acggccatca
    24301 aggtcacata ccagaagaag gctgtttcca ttcctattga tttcagcgac aacgctacgt
    24361 actccgccga accaccaaat atagcattag cgaccgcata tgacaggcca acgcctaatg
    24421 cgcgaacctg tgccgggaac atctcagcct tcagtattcc actgattgat gtataaaaac
    24481 tcactatcag cagggcacac atcaccagac caaaagcggc ataaggcgag gaaacgtttt
    24541 gcaatgctga gagaatagga acggtaaaaa tggctgccag cgaaccgaaa cataacattg
    24601 aggtacggcg accaatctta tccgacagcg cgccaatgag tggttgaata agcatgaata
    24661 caaacaatgc ggcagtcata atgccactcg ccacgttggc atgcattccc gcagtattta
    24721 ccagatactt ctgcatataa gtggtgaagg tatagaaaca aagggagccc gcagcggtaa
    24781 aaccgagaac catgatgaat gcacggcgat ggcgccataa tcctttcaga gatccagctt
    24841 cttttaaagc gcgcgtttct tgttgcgaag tttcatctaa ctgacgacgt aaccacaacg
    24901 ccacaacagc taacacagct cctaacgcga aaggaatacg ccatccccac tctctgagtg
    24961 cagcgtcttc catggtgtgt tgtaaaacca cgacaaccag tagggctagc agttgtccgc
    25021 cgatcaacgt cacatactga aatgatgcgt aaaaaccttt gcgcccttca acggcaactt
    25081 cactcatata ggtggcgctg gtgccatatt ctccgccaac ggataatccc tgaaataaac
    25141 gcgcgagaag caataatgcc ggagcccacg tacctatagt ttcataacct gggaggcagg
    25201 cgataaccag cgatccgaaa cacatcatac acaccgataa cagcatcgat tttttgcgac
    25261 catgtttatc ggctatgcgg ccaaatagcc aaccgcctat tgggcgcatc aggaatcccg
    25321 cagcaaaaac acctgctgtt tgtagtagtt gagtcgtcgt gttcccggaa gggaagaaaa
    25381 tgtgggcaaa gtagagtgaa cagaacgagt agacatagaa atcgaaccac tcgaccagat
    25441 tacctgaaga ggcccccaca atcgcccaaa tgcggcgacg agtatcacta cttgtcagtt
    25501 tgctgtctgc cgttacagta ctttcagcca tgccattatg tctcctgccg taatccgatg
    25561 cttttgtcgg tcgcttttat ctatttcttt tggtaaagaa aatgttatac atttgttgcg
    25621 tatcattatg caaccttaac catgaattta gttagcagga aaatggttat tgaggagctt
    25681 aaggataaat ttctggtaag gaggacacgt atggaagtag gcaagttggg gaaaccgcat
    25741 ccgttgctga atctggcata tgtgcgagta taagacgcgc agcgtcgcat caggcatttt
    25801 tttctgcgcc aacgcaaaaa ggccatcgtg actgatggcc tttcgcttaa tttgatgcct
    25861 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
    25921 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct acggccgcca ggcaaattct
    25981 gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atctttctct
    26041 catccgccaa aaca
//
LOCUS       DAFAVF010000054        25000 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFAVF010000054 DAFAVF010000000
VERSION     DAFAVF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.054"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..406
                     /locus_tag="LGK42_002209"
     CDS             <1..406
                     /locus_tag="LGK42_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBK2658453.1"
                     /translation="PADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLLIQHPS
                     ATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTVQL
                     IPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            406..1674
                     /gene="umuC"
                     /locus_tag="LGK42_002210"
     CDS             406..1674
                     /gene="umuC"
                     /locus_tag="LGK42_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBK2658454.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1720..2250)
                     /gene="dsbB"
                     /locus_tag="LGK42_002211"
     CDS             complement(1720..2250)
                     /gene="dsbB"
                     /locus_tag="LGK42_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBK2658455.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2396..3937)
                     /gene="nhaB"
                     /locus_tag="LGK42_002212"
     CDS             complement(2396..3937)
                     /gene="nhaB"
                     /locus_tag="LGK42_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBK2658456.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4159..4878
                     /gene="fadR"
                     /locus_tag="LGK42_002213"
     CDS             4159..4878
                     /gene="fadR"
                     /locus_tag="LGK42_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBK2658457.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4930..6462)
                     /locus_tag="LGK42_002214"
     CDS             complement(4930..6462)
                     /locus_tag="LGK42_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBK2658458.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            6792..8090
                     /gene="dadA"
                     /locus_tag="LGK42_002215"
     CDS             6792..8090
                     /gene="dadA"
                     /locus_tag="LGK42_002215"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBK2658459.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8100..9170
                     /gene="dadX"
                     /locus_tag="LGK42_002216"
     CDS             8100..9170
                     /gene="dadX"
                     /locus_tag="LGK42_002216"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBK2658460.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9556..11292)
                     /locus_tag="LGK42_002217"
     CDS             complement(9556..11292)
                     /locus_tag="LGK42_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBK2658461.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11387..12301)
                     /gene="ldcA"
                     /locus_tag="LGK42_002218"
     CDS             complement(11387..12301)
                     /gene="ldcA"
                     /locus_tag="LGK42_002218"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBK2658462.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12401..13012
                     /gene="emtA"
                     /locus_tag="LGK42_002219"
     CDS             12401..13012
                     /gene="emtA"
                     /locus_tag="LGK42_002219"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBK2658463.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13014..13748)
                     /locus_tag="LGK42_002220"
     CDS             complement(13014..13748)
                     /locus_tag="LGK42_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBK2658464.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13949..14203
                     /locus_tag="LGK42_002221"
     CDS             13949..14203
                     /locus_tag="LGK42_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK2658465.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14382..14822
                     /locus_tag="LGK42_002222"
     CDS             14382..14822
                     /locus_tag="LGK42_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658466.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(14901..16598)
                     /gene="treA"
                     /locus_tag="LGK42_002223"
     CDS             complement(14901..16598)
                     /gene="treA"
                     /locus_tag="LGK42_002223"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBK2658467.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(16918..18336)
                     /gene="dhaM"
                     /locus_tag="LGK42_002224"
     CDS             complement(16918..18336)
                     /gene="dhaM"
                     /locus_tag="LGK42_002224"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBK2658468.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18347..18979)
                     /gene="dhaL"
                     /locus_tag="LGK42_002225"
     CDS             complement(18347..18979)
                     /gene="dhaL"
                     /locus_tag="LGK42_002225"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBK2658469.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(18990..20060)
                     /gene="dhaK"
                     /locus_tag="LGK42_002226"
     CDS             complement(18990..20060)
                     /gene="dhaK"
                     /locus_tag="LGK42_002226"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK2658470.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20288..22207
                     /gene="dhaR"
                     /locus_tag="LGK42_002227"
     CDS             20288..22207
                     /gene="dhaR"
                     /locus_tag="LGK42_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBK2658471.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMWRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22307..24955)
                     /locus_tag="LGK42_002228"
     CDS             complement(22307..24955)
                     /locus_tag="LGK42_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HBK2658472.1"
                     /translation="MTPAGNTPGNINLGNDVTVNVNDASGYAKGIIIQGKNSSLTANR
                     LTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDGIIIGHSSTLTATQFTIENSNG
                     IGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNNANGAARFTATDLTIDVQGYSA
                     MGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSANALTVDVTGAAANGVEVRGGT
                     TTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNSIFSGGSYGASAQTATAVVNMQ
                     NTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGARGIYAMTNSQIDLTSDLVIDM
                     STPDQMAIATQHDDGYAASRINASGRMLINGSVLSKGGLINLDMHPGSVWTGSSLSDN
                     VNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFASHGSTAGTFATLNVENLSGNST
                     FIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRNQGSEATTGNEVLTVVKTTDGA
                     ASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPTPNPEPTPAPAQPPIVNPDPTP
                     EPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQSKDGNIWLR
                     SYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIGSTHASPDYSGGDG
                     TARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNGVNANGTANGLSISLE
                     AGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLLGRASMILGY
                     DITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGWNNGVGVSAQYNKQHT
                     FYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6151 a         6240 c         6477 g         6132 t
ORIGIN      
        1 gcctgcggat ctccgcgaaa ttgtgacttt tccgctattt agcgatcttg ttcagtgtgg
       61 ctttccttca ccggcagcag attacgttga acagcgcatc gatctgaatc aactgttgat
      121 ccagcatccc agcgcgactt acttcgtcaa agcaagtggt gattctatga ttgatggtgg
      181 aattagtgac ggtgatttac tgattgtcga tagcgctatt accgccagcc atggtgatat
      241 tgtcatcgct gctgttgacg gcgagtttac ggtgaaaaaa ttgcaactac gcccgacggt
      301 acagcttatt cccatgaaca gtgcgtactc gcccattacc atcagtagcg aagatacgct
      361 ggatgtcttt ggtgtggtga tccacgtcgt taaggcgatg cgctgatgtt tgctctctgt
      421 gatgtaaacg cgttttatgc cagctgtgag acggtgtttc gccctgattt atggggtaaa
      481 ccggtggttg tgctatcgaa taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag
      541 gcgctgggtg ttaaaatggg cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt
      601 ggcgtggttt gctttagcag caattatgag ctttacgcag acatgagcaa tcgggtgatg
      661 tcgacgctgg aagaactatc gccccgcgtc gagatttaca gtattgatga ggcattctgc
      721 gatctgacag gtgtgcgtaa ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca
      781 acggtgctac aacgtaccca tcttactgtt ggtgtgggga tcgcccagac caaaacgttg
      841 gctaagcttg ccaatcatgc ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat
      901 ttatcaaatc tggaacgcca gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg
      961 gggattggac ggcggatcag caaaaaactg gacgcgatgg ggatcaaaac cgttcttgat
     1021 ttggcggata cagatatccg gtttatccgt aaacatttta atgtcgtgct cgaaagaacg
     1081 gtgcgtgaac tgcgcggcga accctgtttg caactggaag agtttgcacc gacgaagcag
     1141 gaaattatct gttcccgctc gtttggtgaa cgcatcacgg attatccatc gatgcggcag
     1201 gccatttgta gttacgctgc ccgggcggcg gaaaaacttc gcagcgagca tcaatattgt
     1261 cggtttatct ccacgtttat taagacgtca ccatttgcgc tcaatgaacc ttattacggc
     1321 aatagcgcgt cggtaaaact gctgacgccc actcaggaca gcagggatat cattaacgct
     1381 gctacgcgat ctctggatgc catctggcaa gcgggccatc gctaccaaaa agcgggcgtg
     1441 atgctggggg atttcttcag tcagggagtc gcgcagctca atttattcga tgacaacgca
     1501 ccgcgccccg ggagtgagca attgatgacg gtaatggata cactgaatgc taaagagggc
     1561 agaggaacac tctattttgc cgggcagggg atccagcaac aatggcagat gaagcgtgcc
     1621 atgctttcac cacgttatac aacgcgaagt tctgatttac tgcgggtcaa ataaatatag
     1681 cggcaggaaa aaagcgctcc cgcaggagcg ccgaagggat tagcgaccga acaggtcacg
     1741 ttttttcgct ttaaacggct gggaaatcac caccagcacc gcgacaatca ggtaagcgat
     1801 aaaaataccg agcagccact gcggcatttc cagacctaaa aaatcccact gacgctcggc
     1861 acaatcgcca gaggcgacaa acacttgcgg cacccattta tccagcggca gccattccgg
     1921 gaaacgaacc ataaaatcac aggtggcaaa cggcgaagga tagagctgaa gcatggtgtg
     1981 ctcgtaagtt aactgcacac cgcggaacgc actatacaac cagataacca tcgctacata
     2041 acgcagcgga gttttcgggg cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa
     2101 cgcacaacgt tcataaatac agagcacgca aggtttcagt aacatcacat gctggaacca
     2161 cagcgccgtc agttccagtg ccagagcagt aaacgccatc aacagccacg cgccccggcc
     2221 ttgtgaacat tggttcaaaa atcgcaacat aatcatttcc ctgcaatatg catagagtgc
     2281 gcagtttaaa ccaattcatt cgctgcgcca ccaggaggca gcaaaaatag gaagcaattg
     2341 gacaattatc gggcgaaaag gcaaaccggg catttatatg cccggtaagt tgttatcaaa
     2401 gcgttgctat ccagcccatt tgcataaacc attcggttac aggggcaagc gtaaactcga
     2461 cgcagagcaa gccgacgagt gtcagtacga gggtgtaagg cagggccatc cacaccatgc
     2521 ggccataaga gaggcgaatc aatggcgcga gtgcagaggt cagcaggaac aggaacgcag
     2581 cctgaccgtt cggcgtagcg acggagggca gattggtacc ggtattaatg gcgaccgcca
     2641 gcagctcgta ttgcttcaac gtgatagcgc cactttccat tgccgctttc gcttcgttga
     2701 tataaatcgt ccccacgaag acgttatccg aaatggatga cagcagaccg ttgaaaatat
     2761 agaacagcga cagctgagca tgttccgatg cctgcaacac aaactgaata attggcgaaa
     2821 acagttgttg gtcgataatc accgcgacta ccgagaaaaa caccgtcaac agtgcggtga
     2881 atggcagaga ttcggtgaag gctttaccga tagcatgttc atcggtgaca ccggtcaatg
     2941 atgtcgccag aataatgact gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca
     3001 gcgcagtcac cagccagacg ccaataatcg cctggacaat cagacggatt ttatcctgac
     3061 gggtacgctg gtggcggctt tgatcgtcaa actgttgcaa cacttcgcgg actttctccg
     3121 gcagcgtttc accgtagcca aaccaacgca gcttctctac cagcaggcag gttaacaggc
     3181 cacaaatcag aaccggaacg gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc
     3241 agccagccgc tttagcgatg atcaggttct gtggttcgcc caccatggtc atcacgccgc
     3301 ctaatgcagt gccgacaccg gcatgcatca tcaggctacg cagaaagcca cggaactgtt
     3361 ccagaaccac tttgtaatgc ttgtcgatat gactatcgtc ttgcaggtcg gtatcttcag
     3421 tacgggaaga ggctacgcgg tgataaatac cataaaaacc gactgcaacg ctgatcacca
     3481 ccgccacgac ggttaaggca tcgaggaacg cggagaggaa cgccgccgcc acgcaaaaag
     3541 agagcgacag cagcattttg gagcgaatgc ttaacagcaa acgggtaaat atgaacagca
     3601 atagctgttt cataaaatag atacccgcca ccataaacat cagtaacagc aagacttcaa
     3661 gatttgccgc cacctcttca cggacgtgtt ccgcgctggt catgccgatg aataccgctt
     3721 cgatagccaa cagaccaccg gggagcagcg ggtagcattt cagggccatc gccagagtga
     3781 aaataaattc cgctaccagc aaccagcccg cgacgaaagg gctgatgagg aaaattaacg
     3841 ggtttacgat taagaaaatg atgagggcga gtttgtacca gtcgggggac tggcccaaaa
     3901 aattgcgcca tagcgcgcgg ccccaggaga tctccatgat ggtttccctt accttacaaa
     3961 taatcaatga tgtttttatg tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa
     4021 gtcttgatag tcaaggggaa agagatgcgg aaaatgaagc cttgatccct tttttcttct
     4081 ttttgtctgc tatcagcgta gttagccctc tggtatgatg agtccaactt tgttttgctg
     4141 tgttatggaa atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag
     4201 tacattattg aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa
     4261 cgtgaacttt cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt
     4321 ctggcacgag atggctggtt gaccattcaa catggcaagc cgacgaaggt gaataatttc
     4381 tgggaaacct ctggtttaaa tatccttgaa acactggcgc gactggatca cgaaagtgtg
     4441 ccgcagctta ttgataattt gctgtcggtg cgtaccaata tttcaactat ttttattcgc
     4501 accgcgtttc gtcagcatcc cgataaagcg caggaagtgc tggctaccgc taatgaagtg
     4561 gccgatcacg ccgacgcctt tgccgagctg gattacaaca tattccgcgg cctggcgttt
     4621 gcttccggca acccgattta cggtctgatt cttaacggga tgaaagggct gtatacgcgt
     4681 attggtcgtc actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctaccac
     4741 aaactgtcgg cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc
     4801 tatgggcatg agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta
     4861 gccattcagg ggcgataatc ccttccgttt aaagagcaaa cccctcaaac gaggggtttt
     4921 ttgttgtttt tacagatttc ccattcttgg cgggcaacgt tccagcaact cgatgctgcc
     4981 gtcttcgttt tgctgttcga gcatcacatc aaatccccac aggcgatgca catgcttcag
     5041 cacttctttg cgcccccgat ccagcggtgc gcgattatgt ggaatgtaac gcagcgtcag
     5101 cgaacggtcg ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa
     5161 gttatattgc gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc
     5221 ggaaatctcc agataattat gccgatcatc atccagcacg gtgaagaagc ggaaatcacg
     5281 catcactttc ggtgacagaa actggctgat aaaactctca tctttgaaat cacgcatcgc
     5341 gaaatgcagc gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc
     5401 ttccgttggc gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata
     5461 cgggttgatg ccgctgtacc acgggctgtt ataggggggc tggaagacca cattggtgtg
     5521 gctgtgcaaa aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt
     5581 aaggatggta tagtgccaga aggtcgccca gccttcgttc atcacctgag tctgtttttg
     5641 cggataaaaa tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc
     5701 cagcagtggc gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata
     5761 gcggcgcgct tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat
     5821 attgacctga ctttgtagat actcttcgcg acttttctgc cgggctttct cttcttgcag
     5881 cgagattttt tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga
     5941 gtccagaagc cgttctactt catcaacgcc ataacgttct tcgcactcgg taatatattt
     6001 acgggcaaaa atcagataat cgacaatcga actggcgtcg gtccaactgc ggaataagta
     6061 attgttttta aagaaagagt tatgcccata gcaggcgtgt gccatcacca gcgcctgcat
     6121 ggtaatggtg ttctcttcca tcaggtaagc gatacacggg ttagagttaa tgacgatttc
     6181 ataggccagt ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc
     6241 gaatgaccag tgcggatagt taattggcat gccgacgctg gagtaggcat ccatcatctg
     6301 ttctgaggtt atcacttcaa tctggtgcgg gtaggtatcc agccggtaga gtttcgccac
     6361 ccggtctatc tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct
     6421 caaacgtgtg gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg
     6481 tcggcgctct ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt
     6541 cgccggagaa tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg
     6601 aacggaattt tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt
     6661 gccgcagagt cagggagatg tgagccagct caccataaaa aagccgtatg ttgaataata
     6721 ttttcaactg agttatcaag atgtaattag attattattc ttttactgta tctaccgtta
     6781 tcggagtggc tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct
     6841 ggtacttaaa tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc
     6901 tggaaaccag tgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg
     6961 caccaggtgt gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg
     7021 ttcgtctcga cggtacgcag ttccagttga aatggatgtg gcaaatgtta cgtaactgcg
     7081 acaccagcca ctacatggaa aacaaagggc ggatggtgcg tctggcggaa tacagccgtg
     7141 attgcctgaa agcattgcgc gccgaaacca atattcagta tgaagggcgt cagggtggga
     7201 cgctgcaact gttccgtacc gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc
     7261 tggaagatgc cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggagc
     7321 ccgcgctggc agaagtggcg cacaaactga cgggcggcct gcagttaccc aatgatgaaa
     7381 ccggagactg tcagctattt acccagaatc tggcgcggat ggcggagcag gcgggggtta
     7441 aattccgctt taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg
     7501 tgaagtgtgg cgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact
     7561 cgacggcgat gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc
     7621 tgactattcc aattgcgcag gaagatggtg cgccagtatc cactattctt gatgaaacct
     7681 acaaaatcgc cattacccgt ttcgataacc gcattcgtgt tggcggaatg gcggaaattg
     7741 ttggttttaa caccgagctg ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg
     7801 atctctatcc acgcggcggt catgtcgagc aggcgacttt ctggacgggt ttgcgcccga
     7861 tgacgccaga cggtacgccg gttgtcgggc gtacacgctt taaaaatctg tggctgaata
     7921 ccggtcacgg cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc
     7981 tgctctctgg tcgcacgcca gcgatcccat atgaggatct aagcgtagcg cgctacagcc
     8041 gtggatttac gccatcacgt ccgggccatt tacatggcgc acacagctaa ggaaacgaga
     8101 tgacccgtcc gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg
     8161 tccgccaggc cgcgccgcac gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc
     8221 atggtattga gcgtatctgg agcgcgctcg gggccaccga tggctttgca ttacttaacc
     8281 tggaagaggc aataacgtta cgtgagcgcg gctggaaggg gccgatcctg atgctggaag
     8341 gatttttcca tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac
     8401 acagcaactg gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt
     8461 atcttaaagt gaacagtggg atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg
     8521 tctggcagca gttgcgggca atggcgaatg ttggcgagat gaccctgatg tcgcattttg
     8581 ccgaagcgga acatcctgat ggaatttcca gcgcgatggc gcgtattgag caggcggcgg
     8641 aagggctgga gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag
     8701 cgcattttga ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt
     8761 ggcgtgatat cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg
     8821 gtgtccagac gctaaaagcg ggtgagcgtg tgggctacgg cggtcgctat actgcgcgcg
     8881 atgaacagcg aatcggcatt gtcgccgcag ggtacgccga cggttatccg cgccacgcgc
     8941 ctaccggtac ccctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga
     9001 tggatatgct agcggtcgat ttaacgcctt gcccgcaggc ggggattggt acgccggttg
     9061 agctgtgggg caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct
     9121 atgagttgat gtgcgcgctg gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa
     9181 gccggatcgg agacagcgtc ttctggttgc gaaaaaatca tcaatccggc tggtcagcaa
     9241 ctgtagttgt taatgtgaca gagccattgc ccatgatagt gtccattaaa agaatggaca
     9301 ctatttcccc ggaacctgaa ctcaccgcac aggcgttcta cataaaacgc ttacgcttca
     9361 ttgttgactc gaactcgact tcagataaat cacgctttac ccttgatgga gcctgtacat
     9421 agatttgtgt aattgcctga ttttgatatg ttcaattcaa catcaaatga aggttaaatt
     9481 atggacgaca aacaattgca ggctcaggct gcgttcagca aagcatcgca accggcgata
     9541 gatgcttcat taaatttaag attcagcttc ctcttcagcc acccgtacgc caatcttcaa
     9601 cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc
     9661 accgacaacc ggtgccgcgc ctaacaactg ctggacaatt tcacccagcg tttgctgctt
     9721 atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc
     9781 gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gcggcgactg
     9841 gctgaacagt ttaccgagcg ccggaagatc gcgttcccga ccaattacac acaacacatc
     9901 gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagtgc
     9961 cgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcgccca cgcaccattt
    10021 atcggcactc aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc
    10081 aacgcgtgac accggacgtc ccactggcgg aaccaccact ttggcttttt tagccgccca
    10141 cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa
    10201 gaacagacgt gcattctcca gccccgccat catcgggaac acggccagga tgattggcac
    10261 cgcgccgcgt aatcctaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa
    10321 ggggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgccgacaa
    10381 aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata gccccagcac
    10441 caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta
    10501 gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag ccagaatacc
    10561 gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta atgccagcaa
    10621 tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta aataaccacc
    10681 gccaagtccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca cgaacatcca
    10741 actgacactg ctttcatgtt gctggatcat cgcaatcagg gtaatcgtca gaaagaccgc
    10801 cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc
    10861 cttaccaccc agcagagaaa agaccgctgc agcatcggta gagccgacga tagcgccgat
    10921 taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc
    10981 agaggtgata agcacgccca gcgtcgccag cgacagtgcc ggtcctaacg ccacacgaaa
    11041 ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagtg ccaggttact
    11101 taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcctgc
    11161 cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga
    11221 actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt
    11281 tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata
    11341 ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa
    11401 caggatgacc gctaatcgtc aactgagtgc cttcccgggt gttattcaga atggcatgtg
    11461 cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg
    11521 gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat
    11581 aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa
    11641 ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc tcgttaatat
    11701 cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca atcagtgaaa
    11761 tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc gggccttccc
    11821 cttgccattc aatggtgaag gttttattgc gtaacgccaa ccagaaatgg tgctcggtaa
    11881 aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta aaggtgatga
    11941 cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga
    12001 gtaacgggtc atgttgttgg cgagcaacca gtgcctgcca gtcaatatct gccagtaaac
    12061 gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta gtcagtctgg
    12121 caagggaatt gagatcttcc aggcgctccg tttccgtacc cgcaaaacgc tcacaacgac
    12181 gggcaataac ctcgacgtta ttgacctgat gcccctcgtc ggtaaggcga tggataccac
    12241 gcagcgcggc gtgctgttta atgcagtaac ccgatggggc aattaagtga aacagagaca
    12301 tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt
    12361 gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc
    12421 ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga acccgccgtg
    12481 gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg
    12541 tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg
    12601 taatccgaat gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac
    12661 ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct
    12721 gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg
    12781 cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg tgtcatacgc
    12841 taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa
    12901 aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc atcctgcgcc
    12961 gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg
    13021 cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat ccgctgtaat
    13081 tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg
    13141 gtttcattct tgccatcaat cactttgcgc tcgctgatgg agattaactg ggcgtcaaag
    13201 tgaaaaacac cccattgccc catgttgact tcaatctgag cgaagcgcat gccttcttgt
    13261 aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac
    13321 aggcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg
    13381 agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt
    13441 acggtgataa atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac
    13501 tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg
    13561 atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt
    13621 attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc
    13681 aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa
    13741 tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg tcacagttaa
    13801 caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacata
    13861 cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg
    13921 tccgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct
    13981 gatagccggc attatcgcca agctaatcat gccggggcgt gatggtggtg gatttttcct
    14041 gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg
    14101 cattggcggc tccatcagtg gttttaatct gcacagcttc ttggtggcgg tggtgggagc
    14161 tattctcgtt ctgggcatat tccgcctcct gcgaagagaa taagattttc ataaggcgga
    14221 tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac acctgcttta
    14281 cgggtgaaaa aaaatcaata tagcacttcg atatccgata aagtcggttc ccggtaacaa
    14341 caacagaatc cttttttaat tattgtttcg ttgtttttgg tgtgattcag aaaataaata
    14401 ttttagacag tagccagtca ggcgttaaaa atggggcgga tagtgaggcg gttttggctg
    14461 cgcttattga acatttagaa ttgatcaatc cttctggcag attaagccaa aatacccgtt
    14521 cggcaatgct tcagttacgc gaagaggaat ggtctgaatt ctttttttgg ttgctaaatt
    14581 ctctggagtg tctggattat gtgattatta atttaactcc agaaagcaaa aaaacgttga
    14641 tgagtgaaca ccgtaacaat attcaggtag caattgatgc tttatatagc caacgtcggc
    14701 gtaaatcgcc gggtgatgaa agtgaaacac ttacccgtag aaatgacgct atcttcggca
    14761 accacgtatg gcaaacattt gcgcaatact tcccgccagg acttgaaaaa ccgtctgtgt
    14821 agaaaaaacc atagctatgg tgttatctgc cgacaacgcc atccggagaa gccgaatgac
    14881 gttgtcggag taagcgctgg ttaaggtgtg ggttgtgcct ctttggttga gggttgcgtc
    14941 gttgctgact taacggtcgg acgcgtcgcc ggaacattgt cacacggttg ctctttcggg
    15001 cagatcaaat ccagcatttt cagcgtcacg ccattggtcc agccaaagcc atcctgtaat
    15061 ggatattcgc caccgccgcc ccccgttccg gtggtgctga catcatattt ttccaccagc
    15121 tttttctccc ggtcataggt gtgctgaaca ttggtcagga agtgccagct aatgtccatc
    15181 gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg taacggtgcc
    15241 cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt caggccgccg
    15301 ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc tttcgctgcc
    15361 gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac tttatgactt
    15421 ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acatgtattt ttcgatccct
    15481 ttttgacggg catttgccag cgtttcgtac tggtttgcca tcgcgttatc tccggcagct
    15541 ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag atcgaccggt
    15601 acgatgctgg tggtgcgtaa ggtatttagt tgatgcgggt tgtccatcca gcgcgagctg
    15661 aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt ggcaggtcga
    15721 ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg tggcgtatcg
    15781 cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gttttacaac gcgtttttcc
    15841 tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc tttttgcatt
    15901 tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa ctctaccatc
    15961 agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc gttgggaata
    16021 tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac tttatcccag
    16081 tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata tacctcgcga
    16141 aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc ccatttttcg
    16201 gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc gcgcagtgac
    16261 tgcccctctg gcggaacata tttctcgccc tctttcggca gggtgaaatt gacgttaacg
    16321 aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc aaggatcatc
    16381 agcggatcgc tgttcggcac ggcatcggca aaggtttttt ggtccggaaa aagtttggcg
    16441 ttttgcacat cattaaacag cggccctaat aaaatatcag gcggctgtgg tgttaccggt
    16501 gtttcttctg cctgcaccga tagcgcagcg aaacacaaaa agatacaggc tggaattaac
    16561 gccatttttt gcgggcgaga aggtgcgggg gatttcatca atcattctcc tttggcgaaa
    16621 ccgaataaaa cggtattcaa gtatcagaaa accttagttc aggatcgcac tagctgcatg
    16681 atcgaaatct cattctgcga actgtcaggc attttagaaa agggaattaa agggataaat
    16741 aacggtgaaa tagagtgatc aaataacgtc tgtttattag ccagtcagcg acgttgggtt
    16801 cgcgattagc gaagaaaaat catcataaat tggtaaaaaa tataacaggt agttaaaaaa
    16861 catcagtgct gggtaaattg ccggatgaca tcagaacgat gccatccgaa cggtggctta
    16921 accctgacgg ttgaaacgtt gcgttttaac gtccagcgtt agcgtttctt ctggttgtat
    16981 cgcatacagt ttctcaccct gctggcaaat ccagccaatc cccagttcac gggcgattag
    17041 ggcgctgtgg gataccggac ttccggcgct aaggcagata ccttttacaa ccgccggatc
    17101 cagttgcagt actgtggaag gataaatgtt ctccgccagt agaatagttg gcgagttaaa
    17161 ctgcgggagt tcttctttcg tttgggtcag gtggaccagg gtgcgatgca gaagatcgtc
    17221 cacatcaata tagcgagctt gtagatattc atcatccagt tgctggtatt gctggctaag
    17281 ttctttaaga acttgctgcc aggcatattc tgccgtgcaa tgttcatgct gaaggagttc
    17341 gcttgccgcc gccagcagtt ccggatcatc taacagtgta tggtgaccag aaaagattgc
    17401 ggcaatatcg tcaagcccgc tggcttctgc ttttgctgtt aacgtcatca gatctaataa
    17461 cgtgaagtca atagcctggc gtaatcgatc ttgttcttct tccacggtca gggttgattt
    17521 tgcctgtacc gtacataaaa ctggttgata ataaaaggct ttacccgaaa caggcggaac
    17581 gggacgcaga gtaggtggag cgacttcctc cgtttcacca aagttatctt cagccagctg
    17641 acggaaagcg atcagtgcct cttcagcttc tggccctttc gcaatcaggc gcagcgtatc
    17701 gttatagcga acttgtagta acgcaatctg gttaatactc tctggtgtga cgcatttgcc
    17761 gtttttttcc agcaacatat cggcattaaa tgtcgataag gtataaacca gccgggaggc
    17821 cggacgtaca tgcaggccgt tacggttttt tatgaccacc gccagagaac gggcttcttc
    17881 atcgtacgca ggacatgtgt cagagatttt agtgtcggag gacggtaaac ccagttgttc
    17941 acgtttggct tccagcgcat gcatagcgtc aaagataact ttgtcgatat ccgctcccga
    18001 ggccgcgctg accgttgctg ccagtgtacc ttcgaccaac ggcgcagcac acaaacgtac
    18061 ttttgcggcg atcttgggag ccagcaattc cagcgcagtt tcagcactca ataatgcgct
    18121 acccatatcc atcatgacca gcacatggtc ggcatcagca acagattcga tggcctccat
    18181 cactttgacg gcatcggtac caataggatt ttgtggatcg tcaattcccg cggcaatggc
    18241 gattttacaa ctatcactca ttaacatctg acgggctaat tcaccgacac cttctcccag
    18301 tcggctgcta tgtgaaacta tgaccaggtt taccatcacc aattccttac tcttttgcgg
    18361 ctaacgccaa catttgcatc ataaacatca ccgaggtcgc gccgggatcc tggtgaccaa
    18421 tactgcgttc accgagataa ctggcgcggc ctttgcgggc ttgcatcgta atcgtacttt
    18481 gtgcagcgga ttcggcgatt ctactggcag cttcgagcgc caccggaaca gagagatttt
    18541 gctcgctgga ctgacgtaac gattccacca ccggcaccca cacatcacac atggttttat
    18601 cgccaggttc ggctttcccg cgactgatta cgccgtccac gccatcgcgg aacatctgat
    18661 aaagctcttc cagtgtcagg ctttgccgtg cctgggtcgc ctgtgcggcg cggataaaga
    18721 aggtaccgaa cagcggacca ctggcaccac cgacgctgga aagcagcgtc ataccggtat
    18781 tcttgagaat gaaaccgata tctttatctg cgatagcagg gagtttttcc accactttgc
    18841 taaagcctcg gttcatattt agcccgtggt cagcgtcgcc aatttcgcga tccagtccgg
    18901 taagatactc gctctcggtg ctgaaaatat cgccacaacg agtgagccag ttaacaattt
    18961 gagttctgct cagtgacatt gctttctcct tatttacccc agttaagggc cggggtgtgg
    19021 accggggcgt cccagagtgc cagcgtttcg tcatcaactt tcagtaaggt gattgagaaa
    19081 ccggtcatat ccagtgaggt gcagtacgcg ccaattaaat tacgttcgat agtcaatccc
    19141 gcttgctggc aacgtgtggt caggcggtta tagacgccgt acagctcaga aagcggagtt
    19201 gcgccaagat tgttaaccag cgcaatcacc cgatcgccag actggagcgg ttgtttggtt
    19261 tgttgttctt cctgccaact gccttgttga taatcccaga aacgcaaagt gcgatggtat
    19321 gagccatttt ccagcagggt gtcgaacatt tcatcgacgg tttgatcaag ggaagagaag
    19381 gagcggcggt caatacccgg ctcaccatga atgccgacgc caaactccat ctcattatcc
    19441 gccagggtaa aagaaggttt gcccgcggca ggaacggtac aggcaccgag agcgatacct
    19501 attgagtggc cttgattatt caacttacgc cccagttccg cacaggcgtc cagtgagtcg
    19561 ccacgctccg ccgctgcgcc tacgagtttt tcaattaata cggtgttggc aacgccgcgt
    19621 cgcccggcgg tataaagact gtcttttacc gcaacgtcgt catcaatgac cacagtggtc
    19681 acttttacgc cgctatcgtg cagtaactcg gtcgctgttt caaagttaag aatatcgccg
    19741 gtgtaatttt tgataatcaa cagtacacct tcgccgccat caatttgcat ggagcattca
    19801 aagattttat cgggcgtcgg tgaggtgaaa atttcgcccg gacaggcccc cgaaagcatc
    19861 ccctgaccga tataaccaca gtgcatcggc tcgtgtccgc tgccgccacc cgacagcagg
    19921 gcgacttttc ctgcaacagg ggcatcagct cgggtgacat acaccggatc ctgatgcagt
    19981 gtcagcgatg gatgcgcttt cgccagtcct gccagttgtt cgtccagtac gtcttgcaca
    20041 tcattgatca attttttcat tattttgctc cagcaattac ggtagggcat ggatgatgtt
    20101 caacgacacg gcgaccactg actgccgatg aatccattgt gcatcaggca agggaaaaga
    20161 aaattagccc aaattatgtt tcatagtgaa acatatgctt taatgaatgt tccatattga
    20221 aacttttacg tgtattaata cttaaaattg cgagccggaa caccttttgt cataagggat
    20281 gcgggatatg agtggcgctt ttaacaacga tggtcggggc atatctccct taattgcaac
    20341 ctcctgggag cgatgcaata agctgatgaa acgggagaca tggaacgtac cacatcaggc
    20401 ccagggcgtg acatttgctt ctatttatcg gcgtaagaaa gcgatgctga cgctcgggca
    20461 ggctgcgctg gaagatgcct gggaatatat ggcaccgcga gagtgtgcgc tgtttatcct
    20521 cgatgaaacc gcctgcattc tcagccgtaa tggcgatccg caaaccttgc agcagctaag
    20581 tgcactggga ttcaatgacg gcacgtattg cgccgaggga attattggta cttgtgcact
    20641 atcgttagcg gctatctctg gtcaggccgt gaaaacgatg gccgatcaac atttcaaaca
    20701 ggcactctgg aactgggcct tttgtgcaac gccgttgttt gacagcaagg gccgattgac
    20761 gggaacaata gcgctggcgt gtccggttga gcaaactacc gcagctgatt tgccgttgac
    20821 gttagcaatc gcccgcgagg tcggtaattt gctgctgacg gacagtttgc tcgctgaaac
    20881 taaccgtcat ttaaatcaac ttaatgccct gttagaaagt atggatgatg gcgtgattag
    20941 ctgggacgag cagggtaatt tgcaatttat taatgcccag gcggcgcggg tcttgcgcct
    21001 tgacgcgacg gcaagtcagg gaagggcaat cactgaactc ttaacgttac ccgccgtatt
    21061 gcaacaagca ataaaacagg cacatccgct caaacacgta gaagcaacct ttgaaagtca
    21121 gcaccagttt attgatgcgg tgataaccct taaaccgata atagaaacgc agggaaccag
    21181 ttttattttg ttgctccatc ctgtggaaca gatgcggcag ttgatgacca gtcaattagg
    21241 aaaagtcagc cataccttcg ctcatatgcc acaggacgat ccgcaaaccc gccgcttgat
    21301 tcattttggt cgccaggcgg cgcgcagtag ctttcctgtc ctgctttgtg gagaagaggg
    21361 cgtgggcaag gcactgctaa gtcaggcaat tcataatgaa agcgagcgtg ctgcaggtcc
    21421 ttatatcgcc gtcaattgtg agttatatgg tgatgcggca ctggcggaag aatttattgg
    21481 tggcgatcgc acggacaatg aaaatggccg tctgagtcgg ctggaactgg cgcacggcgg
    21541 cacgctgttt cttgaaaaga ttgaatatct ggcggtggag ttacagtctg ctttgcttca
    21601 ggttatcaag cagggtgtta tcacgcgact ggatgcgcgg cgcttaatac caattgatgt
    21661 caaagtgatt gccacaacga ccgcggacct cgcaatgctg gtggaacaaa atcgttttag
    21721 tcgccagctg tattacgcgt tgcatgcatt tgaaattacc atcccgcctc tgcgtatgtg
    21781 gcgtggcagc attccggcgc tggtgaataa caaattacgc agtcttgaaa aacgcttctc
    21841 tacgcggctg aaaattgatg acgatgccct cgctcgcctg gtttcctgtg catggcctgg
    21901 caacgatttt gaactttaca gcgtcatcga gaatcttgct ctgagtagtg ataatgggcg
    21961 cattcgcgtc agtgatttgc cggaacatct gtttaccgag caggcaacag atgatgtcag
    22021 cgccacacgc ctttccacca gtctgtcatt tgcggaagtt gaaaaagagg caattattaa
    22081 cgcagcccag gtcacaggcg gtcgcattca ggaaatgtcg gctttacttg ggatcggccg
    22141 cactacgctg tggcggaaaa tgaagcaaca tggcattgat gcagggcagt ttaagcgccg
    22201 ggtatgaaag acagagacga tttctgatac atcagagtga tctatatttc attccggcgc
    22261 acgctaacat ttttcagcat cgtttaaggg ctggtctatc ccgcacttaa aagctgaagc
    22321 gatatcctcc gttgacttgc ttctgatcaa agaggttact ctgcgtgtaa tccgcttcga
    22381 ggtagaatgt gtgctgtttg ttatactgtg cactgactcc cacgccgtta ttccagccat
    22441 tacctttgaa actgtacttc tcccgggagt tgttcaacag atagtctgta tcccctgaaa
    22501 actcgcggat agctccagtc ttcacataca tattcagctg gctgttgcct gcggtgatgt
    22561 catatcccag tatcatgctg gcacgcccca gcagcgattc gtagtgattc agatgtatat
    22621 tgagaccatt actcgccttc atagccatct cattctggtg gctgtatgtg agctgggttt
    22681 gcggctctat atagaaccca taaccagtag gggtcaggtt gaacctctgc ccggcttcca
    22741 gggagatgct caatccattc gcagtgccgt tggcgttaac gccgttgttc tgactgtcca
    22801 gtacgtgaaa actatttttc tggcgcgatg cttttacaac gagatcgctg taaaaaccgt
    22861 tgtgtgccat gtaactggca tacattccca tgtagtctga acgtgcggta ccgtcgcctc
    22921 cgctatagtc cggcgatgca tgtgttgagc caatatacag accgacatac aacggcatta
    22981 catcagagag acgtttatcc ccaccaaact ggataccgct gtaacccatg tcaaagccgc
    23041 tcagtttgcc actggcgaag gagtccaggc ttcccccata actgcgcagc cagatattac
    23101 cgtctttact ctgatttcgc aggtcaccca ttcgttgcat cagcgtacgg ttttcaacat
    23161 agtttaataa gtaaccgaca ttgagataat tgccgccagc atctgcggtc gtggtgggtt
    23221 taggtgtggg agtggggtta ggcgtaggat cgggttcagg cgtaggatcg gggttgacta
    23281 tgggaggctg agcgggagcc ggtgttggtt caggattagg agtcggttcc ggaactgtcc
    23341 cggaagcgta aagctcccag ttagtgccat ttttacgcac atcgtacaga tatcccccca
    23401 actcaacctg agaagacgcg ctgaacgagg ccgcaccgtc agtggttttt accactgtca
    23461 gaacttcatt tcccgtagtg gcctcgctgc cctggttgcg gatagccaat acgtgattac
    23521 cagcactact tccgctgata ttcaataaat cccctttatt attaacgcca ttaccctcgc
    23581 caacaacatc agcacgcata ataaaggtac tgttaccgct caggttctct acgtttaatg
    23641 tggcaaatgt gccggcagtt gacccgtggc tggcaaaatc gacagttgaa tggctcagcg
    23701 ccagcgtgtc gagattagaa ttacttgtta cgttccagac gctgttattc attgcaacgt
    23761 ccagtttccc gccattgaca ttatcgctga gggaggaacc tgtccaaacc gaccctgggt
    23821 gcatatctag attgattagc ccacctttgg aaagaacgct accgttgata agcatacgac
    23881 ccgaggcgtt gatgcggctg gcggcataac catcgtcatg ttgcgttgca atggccatct
    23941 ggtcgggtgt actcatatca atgaccagat cgctcgtgag gtcgatctgg ctgttggtca
    24001 tggcataaat tcccctggct cctgccgcgc cggtgatagc caaactgtct ccggttattc
    24061 taccgccgct gagcgcccac aaacccagcg ccagactgcc attacgatca actgtaatat
    24121 cggtattttg catgttgaca acagccgttg ccgtctgggc cgaggcacca taagaaccgc
    24181 cggaaaagat gctgtttcgt tgcgctgccg tgccagaaaa attgattgtc gcgtctgaac
    24241 cactggtgac gaggccaccg ccctgcgcgg aagaaatatg gctatctgca ccgatagtgg
    24301 ttgtaccacc acgaacttcg acgccattgg ccgcggctcc agttacatca acagtgagtg
    24361 cattcgcgct aacctggcca aagctccaga ggccgtgtgc attatcgcca ttggttttaa
    24421 tggtactgtt tgttccgaga tcgacaacag agtttttctg tacgtttatc cccatggcgc
    24481 tgtagccctg aacatcgatt gtcaggtctg tcgccgtaaa acgcgcagca ccattggcgt
    24541 tattgccgtt gagaccaccg atataaacac ctgtacttcc atcggtcgtg attttacttc
    24601 cgcttccaag atcgacactg gtgccatagt cattgatggt taggcctata ccgttcgagt
    24661 tttcaatggt gaattgagtg gctgttagtg ttgagctatg cccaataatg atgccgtcat
    24721 cgttactctt aatggtgctg cctgtgccta agtcagcatg ggtatagtca ccaattaagt
    24781 taatgccgat ggcagaggtt tgaccaacaa catctactgt gagtcggtta gctgtcaggg
    24841 agctgttttt gccctgaata atgattcctt ttgcatatcc agaggcgtcg ttgacattga
    24901 cggtgacatc attacccagg ttgatattgc caggcgtgtt tcctgctggg gtcattacac
    24961 catagaggtt tcctgatgga tcggcggtat ctcctgtaat
//
LOCUS       DAFAVF010000055        24359 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFAVF010000055 DAFAVF010000000
VERSION     DAFAVF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.055"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(227..952)
                     /gene="phoU"
                     /locus_tag="LGK42_002229"
     CDS             complement(227..952)
                     /gene="phoU"
                     /locus_tag="LGK42_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK2658473.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(967..1740)
                     /gene="pstB"
                     /locus_tag="LGK42_002230"
     CDS             complement(967..1740)
                     /gene="pstB"
                     /locus_tag="LGK42_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBK2658474.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1831..2721)
                     /gene="pstA"
                     /locus_tag="LGK42_002231"
     CDS             complement(1831..2721)
                     /gene="pstA"
                     /locus_tag="LGK42_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK2658475.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2721..3680)
                     /gene="pstC"
                     /locus_tag="LGK42_002232"
     CDS             complement(2721..3680)
                     /gene="pstC"
                     /locus_tag="LGK42_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBK2658476.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3767..4807)
                     /gene="pstS"
                     /locus_tag="LGK42_002233"
     CDS             complement(3767..4807)
                     /gene="pstS"
                     /locus_tag="LGK42_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK2658477.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5055..6117)
                     /locus_tag="LGK42_002234"
                     /pseudo
     CDS             complement(5055..6117)
                     /locus_tag="LGK42_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(6128..8650)
                     /locus_tag="LGK42_002235"
     CDS             complement(6128..8650)
                     /locus_tag="LGK42_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2658478.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(8675..9406)
                     /locus_tag="LGK42_002236"
     CDS             complement(8675..9406)
                     /locus_tag="LGK42_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2658479.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9454..10026)
                     /locus_tag="LGK42_002237"
     CDS             complement(9454..10026)
                     /locus_tag="LGK42_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBK2658480.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            complement(10328..12157)
                     /gene="glmS"
                     /locus_tag="LGK42_002238"
     CDS             complement(10328..12157)
                     /gene="glmS"
                     /locus_tag="LGK42_002238"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK2658481.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(12319..13689)
                     /gene="glmU"
                     /locus_tag="LGK42_002239"
     CDS             complement(12319..13689)
                     /gene="glmU"
                     /locus_tag="LGK42_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK2658482.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14040..14459)
                     /gene="atpC"
                     /locus_tag="LGK42_002240"
     CDS             complement(14040..14459)
                     /gene="atpC"
                     /locus_tag="LGK42_002240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK2658483.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(14480..15862)
                     /gene="atpD"
                     /locus_tag="LGK42_002241"
     CDS             complement(14480..15862)
                     /gene="atpD"
                     /locus_tag="LGK42_002241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK2658484.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(15889..16752)
                     /gene="atpG"
                     /locus_tag="LGK42_002242"
     CDS             complement(15889..16752)
                     /gene="atpG"
                     /locus_tag="LGK42_002242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK2658485.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(16803..18344)
                     /gene="atpA"
                     /locus_tag="LGK42_002243"
     CDS             complement(16803..18344)
                     /gene="atpA"
                     /locus_tag="LGK42_002243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK2658486.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(18357..18890)
                     /gene="atpH"
                     /locus_tag="LGK42_002244"
     CDS             complement(18357..18890)
                     /gene="atpH"
                     /locus_tag="LGK42_002244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK2658487.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(18905..19375)
                     /gene="atpF"
                     /locus_tag="LGK42_002245"
     CDS             complement(18905..19375)
                     /gene="atpF"
                     /locus_tag="LGK42_002245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK2658488.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(19437..19676)
                     /gene="atpE"
                     /locus_tag="LGK42_002246"
     CDS             complement(19437..19676)
                     /gene="atpE"
                     /locus_tag="LGK42_002246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBK2658489.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(19723..20538)
                     /gene="atpB"
                     /locus_tag="LGK42_002247"
     CDS             complement(19723..20538)
                     /gene="atpB"
                     /locus_tag="LGK42_002247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK2658490.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(20547..20927)
                     /gene="atpI"
                     /locus_tag="LGK42_002248"
     CDS             complement(20547..20927)
                     /gene="atpI"
                     /locus_tag="LGK42_002248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBK2658491.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(21544..22167)
                     /gene="rsmG"
                     /locus_tag="LGK42_002249"
     CDS             complement(21544..22167)
                     /gene="rsmG"
                     /locus_tag="LGK42_002249"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK2658492.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(22231..24120)
                     /gene="mnmG"
                     /locus_tag="LGK42_002250"
     CDS             complement(22231..24120)
                     /gene="mnmG"
                     /locus_tag="LGK42_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK2658493.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
BASE COUNT         6073 a         6350 c         5829 g         6107 t
ORIGIN      
        1 caggttttgc ctgcgaatgc agtaacaatc cagtcattta ttaatggttt ttataacgaa
       61 catccaggtt cggaaattaa tttaattgcg tgcagagaaa atagcaatgc gctattgata
      121 aaaatatgac catgctcgca gttattaact ttgtgtaatt ttaggaattt ataaagttat
      181 atataacaaa tcccaataat taagttattg ggatttgtct ggtgaattat ttgtcgctat
      241 ctttccccgc cagcagttta tccagctcat cgccaccgac gtgacggaaa tcctgcccct
      301 tcacgtagta gaagataaac tcacaaatat tctggcagcg gtcgccaata cgttcgatag
      361 aacgcgcgca gaacagcgca gtaagtacgc tcggaatggt acgcgaatct tccatcatgt
      421 aggtcatcag ttgacgaaca ataccttcgt attcctgatc gactttttta tcttcacgat
      481 aaatacgtac cgcttcgtca atgtccatcc gcgcgaacgc gtccagcacg tcgtgcagca
      541 tctggatggt atgacggccc agcgactcca gacttaccag caacggctga tgctgctggg
      601 agaatttctc cagcgcagta cggcagattt tgtccgccac gtcgccaata cgctccagct
      661 cggcaatggt tttactgatc accataacca gtcgcaggtc gctcgccgtc ggctgacgtt
      721 tggcgataat gcgcacgcag gcttcatcga tcgccacttc catcatgttg acgttcttgt
      781 cgccttcgat cacgcgcttc gccagatcgc tgtcctggtt atgcatcgcg gtgattgcat
      841 cagaaagctg ctgctccacc atgccgccca tggtcatcac ctgcgtgcgg atactttcca
      901 gttcggcgtt gaactggccg gaaatatgtt tattaagatt gagactgtcc ataacgcact
      961 cctgaatcaa ccgtaacgac cggtgatgta gtcttctgtt tgtttcttcg ctggcttggt
     1021 gaacagatcg tccgtgttgc tgaactcaat caattcgccc aggtacataa acgccgtgtg
     1081 gtcggaacaa cgcgcagcct gctgcatgtt gtgggtgacg atcaccacgg tgtaatcctg
     1141 cttcagttcg gtgatcagct cttcaatacg cccggtagag atagggtcga gcgccgaaca
     1201 cggttcgtcg agcagcagca cttccgggcg aatggcgata ccacgcgcaa tacacagacg
     1261 ctgttgctga ccaccagaga gagagtaacc gctctggtgc aatttatctt tggtttcgtt
     1321 ccacaatgcg gctttggtca atgcccactg cacgcgctcg tccatgtcgg cacgggagag
     1381 cttctcaaac agacgaacgc caaaagcgat gttgtcgtag atggacatcg gaaacggcgt
     1441 cggtttctgg aacaccatgc ccactttcgc acgcagcagg gcgatatcct gagagttggt
     1501 caggatgttg tcgccatcaa gcagaatttc accttccgca cgctgctccg ggtacagttc
     1561 aaacattttg ttgaaggtac gcagcagcgt cgatttaccg cagccggacg gcccgataaa
     1621 cgccgtaacc tggtttttag cgatatccag gttgatgttt ttcagggcat ggaatttgcc
     1681 gtagtagaag ttcaaattac gaacctgaat tttactcggg gcagtttcaa ccatactcat
     1741 tgcaatcttt tcctcatcgt gcctgatgca cttcgctaat caggcttacc gtaggccgga
     1801 taaggcgttc acgccgcatc cggcaaagtg ttaaccgtgt ttattcttcg caaaaacaac
     1861 gcgcgccaga atgttcagca gcagtacgca cagggtaata atcaataccc cggcccaggc
     1921 cagttgttgc cattccgcaa acgggctcat cgcaaactta aagatcgtca ccggcaggtt
     1981 ggcgatcggc tgcatcatgt ccgtgctcca gaactggttg gagagcgcgg taaacagcag
     2041 cggcgcggtt tcaccagcaa tacgggcaat cgccagcagg ataccggtca tgatcccgga
     2101 caccgacgct ttcagcgtaa tcgcggagat catcttccac ttcggtgtac ccagcgcata
     2161 agccgcttca cgcaggctgt ccggcaccag tttcagcatg ttctcggtgg tgcggataac
     2221 aatcggcacc tgcaacagcg ccagggcaat cacgcccgcc cagccggaga agtgctccat
     2281 ctgcgccacc acgatggtgt aaacaaacag accaaccaca atcgacggcg cggagagcag
     2341 aatgtcgtta atgaagcgaa tcacttctgc cagccaggat ttacgaccat attccgccag
     2401 ataaatcccc gccataatgc ccagcggcgt accgaatacc gtggcccaca aaattaacag
     2461 cccgctaccc gccagggcgt tcgccagacc accaccttcc gtattgggcg gcggcgtcat
     2521 ttcagtgaac agcgccagcg acataccgtc gataccgcga gtgatggtgg acattaaaat
     2581 ccagatcagc cagaacaggc cgaaggccat cgtcgccatc gagagcgtca gcgcaatacg
     2641 gtttttgagg cggcgacgcg cctgcatttt gcggcgagat tcagccagcg ccgcagtggt
     2701 ttgcatttca accatagcca ttagcgtgcc ccctcattct tagccaggcg cataatcata
     2761 aacttcgatg cggcgaggac gatgaaggta atcacaaaca ggatcaggcc cagttccatc
     2821 agtgcggcaa cgtgcagacc ggattccgct tccgcaaatt cgttcgccag cgcagaggtg
     2881 atactgttgc ccggcatata cagcgaggcg ctgtcgagct ggtaggtgtt accgatgata
     2941 aaggtcaccg ccatggtttc accgagcgcg cggcccagcc ccagcatgat gccgccgata
     3001 acaccatttt tggtgaacgg aagaacgata cgccagataa cttcccaggt ggtgcagcca
     3061 ataccgtagg ccgactcttt catcatcacc ggggtttgtt cgaacacatc acgcattacc
     3121 gccgcaatgt acggaataat catgatggcg aggatcacgc ctgccgcgag gataccgata
     3181 ccaaatgcgg ggccagagaa cagcgcgcca acaatcggga tattcgacat gatattgccg
     3241 accggctcct ggaagtaaac ggcgaatagc ggcgcaaaga taaacaggcc ccacatgccg
     3301 taaacgatac ttggaatggc tgccagcagc tcaatggcga tacccagcgg gcgtttcagc
     3361 cagccaggcg caagctcagt caggaacagg gcgataccga aactcaccgg gacggcgatc
     3421 agcagcgcga taaacgaagt caccagcgta ccgtagatcg gcaccagcgc cccgtagata
     3481 tcgttcggtg catcccactc tttggtccag aggaaagcca gaccaaattt ctgaatgctc
     3541 ggccaggagg agatgatcag agagacaata atgccaccca acatcaatag cacaatcagc
     3601 gccgccagtt ttaccagcac gctgaaaatt atgtcgccct ttttacccgg tgggttaaaa
     3661 gcaggcttgg ttgcagccat aagttactct tcagttaaac gcgtttacaa agtcattttg
     3721 taatgccgga tgcggcgtaa aacaccgtac ccggcctgga gttttattag tacagcggct
     3781 taccgctact gtctttaata ttggtcttcc acgcagcgcg aacctgttca actacactat
     3841 ccggcaggct ggcgtaatcc aggtcgttcg cctgtttagc cccggttttg tacgcccagt
     3901 cgaagaattt cagcacttct gtgccttgtt ctggtttctt ctgatctttg tggatcagaa
     3961 tgaacgtggt agaggtaata ggccatgcat cttcgccttt ctggttggtc agatcctgag
     4021 cgaaggtttt gctccagtct gcaccttttg ctgcattagc gaagttttct tcggtcggac
     4081 taaccggttt accatcagcg gagatcagtt tggtgtacgc caggttgttc tgcttcgcgt
     4141 aagcatattc aacataacca attgcacccg gcagacgctg aacgaacgcg gcgataccgt
     4201 cgttaccttt accgcccaga ccgatcggcc attttacggt agagccagta ccaacgttgt
     4261 ttttccactc ttcgttcact ttcgccaggt agctggtgaa gacgaaggaa gtcccggagc
     4321 catctgcgcg gcgtactaca gcaatgtttt gtgaaggcag tttcagaccc ggattcagtt
     4381 tggcgatggc ttcatcatcc cacttcttga ttttgcccag gtagatgtcg ccgagggttt
     4441 taccatccag caccagttcg ccagacttca gccctggaat gttaaccgcc agcaccacgc
     4501 cgccaatcac ggtcgggaac tggaacagac cttcctgagc cagtttttcg tcagacagcg
     4561 gcgcgtcaga ggcaccaaaa tcaacggtat tagcgataat ctgttttacg ccaccggaag
     4621 aaccgatacc ctggtagtta actttattac cggtttcttt ctggtaagtg tcagcccatt
     4681 tggcatacac cggcgcaggg aaggttgcac ctgcacctgt caggcttgct tctgcaaaca
     4741 cagagaaagc gctcatcgat aaggtcgcgg cgacaacagt tgcgacggtg gtacgcataa
     4801 ctttcataat gtctcctggg aggattcata aagcattgtt tgttggctac gagaagcaaa
     4861 ataggacaaa caggtgacag ttatatgtaa ggaatatgac atttttatga cagaaaggaa
     4921 ttaccaaata acttactgat tttaaatgtg aagatgaaaa tcccacatgt catctttttg
     4981 tcatgatgca tttaaaagtg ataaagaaat gggtgagatg acaacgatgt gatgaacaat
     5041 cgttgtcaga cctattagcg cacaatctca acagttatcg tcgctgttgc agtaaattgc
     5101 cccggggctg ggcgagctcc agtaaggctc gcaggagcag ctgaaaaagt tacactacct
     5161 gatttttcac catacactgt tgatttaccc atgtctgttg gcaactcacc accgttcact
     5221 ttgatttcct tattattttt gtcgtaaatt tttatcccca cgttagagtt tgaagttacg
     5281 accattgaat tgtccgtatt gggttcggta gcataaaaag acgtactgat atcatcggta
     5341 ttcagtatcc ccaggttttc acactcaata gtgaccgttt tagtaatttt atgtgttgtt
     5401 accgcacttc caacaacaga tgaaaaatct gcagctggta tttctttaaa atcaaaataa
     5461 actggctgta aattatttat ttcgcaactt tgcggagctg taatatcgcc ccgaatataa
     5521 acatcagcca tcggctgtga gtaatccacc ggggtgtcac ggctaattgt tccatacaat
     5581 gtcactatct ttgtttcagg aataatcaat tttcctgcaa atgtcttctt gatataaaaa
     5641 tatactatcg ctttagcccc actagatata cttaaaggtt caatgcggtt gcaattgtac
     5701 tttgtattac cagacgggtt gttagcatga tattcaaaag gtgcattcac atacccaacc
     5761 ccaagaatat ctatagcaat cccaacatca aggttcataa taattaatat tagagtgaac
     5821 cccagcataa gttaatgcct ggtttatttt tgacgtataa tagatatagg ggaattctcc
     5881 tacgttacca gtatcacaat gacagcgtcc aggatatgag gatccattag ttacagtggt
     5941 gttaaatgtc gttccaacct tattttcgtt cgcggtaata agatatcccg agaaatcaag
     6001 cgtactgtga aatacgccat tatcaggggt acagatacct ttaggagctg caagtgctac
     6061 actacttaac gagagcataa acacagcaaa gcaccactgt ttgatatact tgttcataaa
     6121 aatcctttta atgacatgtc acaccctgtt caattaatcc gctctcactt tctggtgtcg
     6181 aaagtgaata attgaagatg cattttgttg aggcgttttc tccccaggat aacgtaacct
     6241 gtcctttttc tggtagacca gaaagataaa gttcgccacg gtcaccgaca attcctgaat
     6301 ctgccaaact tgcatcttgt atcgcagcaa tggctccaaa aggtaccggt ttaccatttg
     6361 catttaacac tctgaagaga acgcgataac ccacatgagt cacaaactcc gctttaacca
     6421 ctgcaccacg cgtgggaacc atcgtaacga cggtattatc aagttctata ttcgacttcg
     6481 agaaatagtt atcgctgatt gctacccgat tttcatcata agggtttagc tgtgtcttga
     6541 ccgtatagcc gcgccagtcc gtcgcagcac cgcgcatatt atctatttct aacccggcgg
     6601 ccccaggtgc tttaacaaga gcaatagttt cacctaactc ctgacttaac gttacccctt
     6661 cgctgtgtgc cagaattccc cctctgatac cataattcag cctctgggaa ttttggctgt
     6721 agttatagct gccgttcacc gtgccataag agccatcata ggtcagggac atattaccgc
     6781 caacctcatc atcatgactg ttataggact gcattaaatt ccatgaaagg ttatttcttt
     6841 ccagcgccgt accgctgagt ccgagttgat gtgagctacc ggaattatcg ttttgcgaaa
     6901 cagaataggt ggcgtaacta ttaccccacc agcccgataa aggaacagat aaagagagca
     6961 tgtagatctg ctcactggtg ttatcactgg catggttgtt ccccgtcccc tgtctggcag
     7021 tattggaata actccaggag acgttccacg agagacgctt ccagctactg ctgtagccaa
     7081 attgcattaa tcgttcgaca tcatcggtat gccagtattg ttgccggctt aatgtcaggt
     7141 ataaattgcc ataatctctc cccaacgact gcgttagcgt tccctccacg cgactacgtc
     7201 ggttacctgt ttcccaaaag tcttcagggc tgttgcgccg cgatgcccac tcattgaggg
     7261 tatagtagcc ctcggtggag taacgatagc caaccaggcg caagctagtg tctgtgtcgt
     7321 caaatcgttt tgaatagaga aagcgatatg actgacccgt ttctgtatca tcatggctaa
     7381 aattcgcacg agcatgtgtc acatcgaaag aaattgcgcc caaagcgcca agatctttac
     7441 cgacacccag agctgcagaa ccataatttt cagcagcctg catacctcca tataaagtga
     7501 tcccgtggga aaaaccatga agtatttgcg cctgaaggac gtccggtgta aatccgtctt
     7561 catcgcgcaa ttcacccacg ctgacatcaa catctgtctg accttcacgt ttaagaatcg
     7621 ccaatgaagc ataaggttgg gtgaatcgtt gttcgctacc gtcttcttct ttgatggtta
     7681 cgtccaaatc gccacctgta ctggcggtgt tgagatcggt gatctcaaaa gcaccttgtg
     7741 gaacggcaga ctgataaatc acgtagccat tctgccgaat gatgacggtg gcgttggact
     7801 ttgcaatccc acgaaccaca ggagcaaagc cattctggct ggcgggtagc atatcgttat
     7861 cagtatacaa acgcgcgccg cgaatttgcg tactgtcgaa aatatcgctc gccgtccacg
     7921 tatcaccaat cataatctgg cttcttaacg cggcaatatc gcgctgtatc caggtactaa
     7981 tggagttgta ttccgcacgg ccatcgctgg tgtttaaagt actgtaatta cgtagccgcc
     8041 acggtccaat attcagtcca ttgcgcagat tgagataact ggtatctgtc tcttgtgaac
     8101 cataatctga acgtagtgta tgtgaaccag aaaaatcgta gttgatggtg agcgcgttaa
     8161 tcccctcatc ccaacgcgtg gggctgatgt aatcataagc catcgcgtcc atgtagattt
     8221 ggggaatgga tatattcagc tgtaatttat cactgtcaaa ctgccaggat gagccttcaa
     8281 tacgtggagc aggatcgagg caaaccgtct catccacatc attgaacgat ttgagcgcat
     8341 caacttttat gccaaaggag gacagtgttt ttttaatggc aataaagcaa gcgtgcagat
     8401 cagcatctgg attatcctga acaaatgtca catcctgacg ttcaaacatt ttaccgttga
     8461 taaaaatatc aacgctataa acacctgggg gcatcaggtt tgcccgatta aactggcgaa
     8521 tatcgactcc ggattgatgg tcgatttcca gaagcgccgg attgaataca ctgacctcgg
     8581 ccatcaggtt ttgcgagaaa atcagacacg tccctgccgt gaggccaacc actattctgg
     8641 tcgtcatcat aatacgccta tccttatccc ttgcttacag cgtcattttt atcgggtcac
     8701 tgactgcgcc gtaatcatta ataaatcgcc aggtcatttg gcctcccgca gaattagcag
     8761 gtaaaggtaa cgtgagcgtt tcaaaaggac ggatcatatt gaggggcaca tcatggccat
     8821 caacgatgac atccatcaca ctgatatgaa ttgggttggg attctctgct atcagatttc
     8881 gcccttcccg gcgccatttc actttcggcg cagcatcttc gggaataagg cggatatttg
     8941 ctggtcgcca gaacagcttg atacgcgttt ttactgctat ttccagcgag ttcgtcgctt
     9001 tgttgctcgc tgatggcgga atatttttaa tattcagcca gaagactgac tcgcgatcat
     9061 caggcaatga cacacctttg gtgtgaataa tgcgtaatat tttttcttgc gcaggttcta
     9121 atcgtgatac tggcggaata acggtaaacg gcgctttgct tttatcctct ttattaaatg
     9181 gatctaccca agcctggatg agataaggta ttttttcttc tttgctaaat acagaaatag
     9241 atttatcagc attattactt aagtaaacca cccgcgtacc accaatgaca acgcctgcat
     9301 aagttaatgc tggttggcct aaaaaaacgc tgagtataat cgcggtaaat aacttacatg
     9361 aaactgttgt tttaaagagc ctgattaaac aactttttct ggacataacg actccctggc
     9421 caaagccaac tgaataaaaa ggcccttgat aaattatttc ttatattcga cagtaaattg
     9481 tgaatcagcg ttcgctgtac cagccgttac ggtcgcattg gttgccacat agcgtgcaat
     9541 aaacttcatt tcagccttgc cttcagcatc gatagcggta taagcagagt cgttataaag
     9601 tttaacctgc gaattatcgg actggttaaa aatacgaata ccaacgccgg ttgccgcaat
     9661 cgcttgtcct gtaccggtaa aatcaccagg gttgcctgct gtaagcggcg tacccacttt
     9721 caggtcgccg tcgccgccag gcgcttctgt accatcaaaa cgaacggctg cttgagtgta
     9781 cgttgccggg caattttcga gcttaatact gaacttctgt ggcgacgcca gactacctac
     9841 acctgaaaat gcatttttac tgactttacc cagtgggaca atttgatcgg ctgtatcgga
     9901 ggtaacttca caagtagtgt cgacaatttc accgacgaag tgaacaacac catcttctgc
     9961 cagtgcatgc ccggccagca ttagagatgc cagagtgaat gcaccgccta taatattacg
    10021 cttcataaaa actccattga tataatatat cctttaccat gaatcatcac ttatattaat
    10081 aagtgtattt ttgattcaaa ggaatagtac accaaagaga cagctttatc taaaaataat
    10141 attaattacg caaaaataaa atataaattt attgtttaaa tattatttaa acagtaaaaa
    10201 atcaagtttg ttatcagaca ttaatgataa ttcagaatat atttatatag aaagatagat
    10261 gaatttattg atgtcagtta taaaaaaggg ccaccggtcg gcagcccttt tttatctatg
    10321 atagtgatta ctcaaccgta accgattttg ccaggttacg cggctggtca acgtcggtgc
    10381 ctttgatcag cgcgacatgg taagccagca gctgcagcgg aacggtgtag aagatcggtg
    10441 caatcacctc ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac
    10501 ccgcatcctg atcggcgaag acatacaact gaccgccacg cgcgcgaact tcttcaatgt
    10561 tggatttcag tttttccaac agttcgttgt tcggtgcaac gacgataacc ggcatatccg
    10621 cgtcaatcag cgccagcggg ccgtgtttca gctcgcctgc agcgtaggct tcagcgtgaa
    10681 tgtaagagat ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac
    10741 ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc tgccagagct tcaatgcgtt
    10801 tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt
    10861 catgctcaat ggaggcatcc agacctttca gtcgagacag ttttgccacc agcattagca
    10921 gcacggtgag ctgggtggta aatgctttgg tggaggccac accaatttcc gtaccggcgt
    10981 tggtcatcag cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg
    11041 ccagcgaacc aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg
    11101 tttcgccaga ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttgcgat
    11161 agcggaattc agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt
    11221 agcgggaaac cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa
    11281 ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc
    11341 tgatgcgccc ggtaagggtg tttttgatcg cgtttggctg ttcgtagatc tctttctgca
    11401 tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg cagattggat tcaatatcct
    11461 gacgttttac ttccgcgcca gttttatcga agatgtttac cgaacggcga gtaatttccg
    11521 caatatcgcc ctcttcaagg aagataaagc gacgggtcac cggcaacagc gccagctggt
    11581 cagaagcgat aaagttttcg cccataccca ggccaatcac cagcggacta ccagaacgtg
    11641 ccgccagcag ggtatccggg tgacgggagt ccataatcac tgtaccgtac gcaccacgca
    11701 gctgcgggat agcacgcaga acagcctcac gcagagtccc gccttgtttc agctcccagt
    11761 tcaccagatg ggcaatcact tcggtgtcag tttcagaaac gaaggtatag ccacgcgctt
    11821 ttaactcttc acgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt
    11881 gttcagaaac atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag
    11941 tatgagcaat accggtgccg ccatgcagag gatgttcttc cgctgcctga gccagcatct
    12001 ggactttacc gaggcgacgc aggcgggtca tatgaccttc tgcatcaaca acggccagac
    12061 cggcagagtc atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta
    12121 catcacgttg cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc
    12181 gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg
    12241 ttatttttat agttactgct tgtgggaggg agattatgtt atctcctcat cccagatgac
    12301 cggattaccc ggcctgaatc actttttctt taccggacga cgccagcctt ctttctgagt
    12361 ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc
    12421 cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc
    12481 gacaaacaca tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt
    12541 aatggttccc gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag
    12601 atgcccggct ttcgagcctt tacccagacg cgcttttttc atctcaacga agttaccgac
    12661 gtgtgcacct tccagcaact cagcaccggg acgcagacgg gcaaacgggc caatagtaca
    12721 ggccgctgcc agattcgcgt cttccacaac ggtatacgga ctgatttcgc aatcatcgcc
    12781 aatcacgctg tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac
    12841 gttgccctcg atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc
    12901 gcgcagatca aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc
    12961 ctgttcggac tggtaaacgc gctccagacg agagagttgc aggcggttat tcacgccttc
    13021 aacttcgctt aaacgttgcg gatgaacggc gacgatttca cgtccttcct gatacgccag
    13081 cgcaataatg tcggtgatgt agtattcacc ctgagcgtta ttgttggtca gcttcgccag
    13141 ccagcgtttc atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg
    13201 acgctgctcg tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cgttttcacg
    13261 ggtgatacgt ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc
    13321 ctgcggttta gcatcacgca gacgctggag tgtttccaca gagatcagcg ggacgtcgcc
    13381 gtagagcatt aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcattgcatg
    13441 acccgtaccc agctgctctg cctgaagcac ccagttcagg ttgtcgtctt tcagcgcctg
    13501 ttttagcaga tcgccgccgt gaccgtacac caggtgaacg tgcgctgcgc ctaattcatt
    13561 cgcagcatca atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg
    13621 aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc tcatagcatt
    13681 attcaacata cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa
    13741 aaactacatt tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc
    13801 cccgctgacg aaagtgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag
    13861 gcagtacctt catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa
    13921 acgggaaaac aggggtgttt tatatcagca ggatctatgt gaacgctctt caggacggat
    13981 cacgcgtaaa aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt
    14041 tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc
    14101 gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta
    14161 cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc
    14221 agaacggtca cgttgccagg ctgcacttca agaatgccgc cagacagata gataaactct
    14281 tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt
    14341 gcgtggccag ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc
    14401 agaccagaga acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata
    14461 tcaccctccg attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac
    14521 cgaccatgta gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa
    14581 agccacggat ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg
    14641 ccacgaagaa cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt
    14701 tgtcttcttc agacagttca tccataccca ggatggcgat gatgtctttc agttcctgat
    14761 aacgttgcag gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca
    14821 gcgggtccag ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag
    14881 acgcgatctg acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag
    14941 acgggtcagt caagtcatcc gcaggtacgt atactgcctg tacggaggtg atagaaccag
    15001 ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat
    15061 aacctaccgc tgaaggcata cggcccagca gtgcggatac ttccgtacct gccagggtgt
    15121 aacgatagat gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag
    15181 ccatggtcag accggtcaga gcaacgcgca gacggtttcc cggcggctcg ttcatctggc
    15241 catacaccag ggatactttg tcgataacgt tggagtcggt catttcgtgg tagaagtcgt
    15301 taccctcacg agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg
    15361 cgatgttacg aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca
    15421 gaccaacttt accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt
    15481 ccagcagttc ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc
    15541 aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga
    15601 tacggcccag agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat
    15661 ccagaccgcg acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccgc
    15721 cgagctgctg ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat
    15781 cgtacacgcg cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct
    15841 ggacaatctt tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc
    15901 ggcccccgag acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta
    15961 taccaactgc agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac
    16021 catacgggcg gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc
    16081 gacataacga cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc
    16141 ccaggattta tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat
    16201 ggtcggaacc tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag
    16261 acggccttcg tcgtaggcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga
    16321 agggttatcc cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa
    16381 cgacacgcct ttcgagccga tcattgcgag gtcgcattga acgcctttgt cggtccaggt
    16441 cttcatttcc gccagcagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg
    16501 gtcggtcgac accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg
    16561 cttatattcc agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata
    16621 aggacggctg gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc
    16681 catcgcttta gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt
    16741 tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga
    16801 cgttaccagg attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt
    16861 tcgtcgttgt agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg
    16921 acgtaagcca gcagagcggc ttcgaagctg ccaattttcg acagttcaac atccgccagg
    16981 taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac
    17041 tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt caagctgctt acgtgttgca
    17101 tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg
    17161 gtacggatac caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg
    17221 gatacggaaa tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc
    17281 aggaagatct gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca
    17341 cccgcctgag tttcgataat aggcagtgcg gtcagagaac cggttttccc tttcacttca
    17401 cctttggtga aggcttcaac gtattcggcg ttaacacgtg cagcacgctc cagcagacga
    17461 gagtggaggt agaaaacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc
    17521 agggagatct gacggtaagc aacagcctgt ttagacaggt catcgtaaat gatcagcgca
    17581 tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac cggcatacgg tgccaggtat
    17641 tgcagtgcag cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg
    17701 tgctcttcca gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca
    17761 tagatacatt tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt
    17821 ttacctgtct gacggtcacc gatgatcaat tcacgctgac cacgaccgat tgggatcatg
    17881 gagtcaacgg ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg
    17941 cccggagcga ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg
    18001 attggtgcac ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc
    18061 aggatacggc cagtacactt aactttcatg ccttcggcaa ggtcagcgta cggacccata
    18121 acaaccgcac ctacagagtc gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc
    18181 agggagatca tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca
    18241 cttacagaaa caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg
    18301 cgctgcttga tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa
    18361 gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata
    18421 tcacccgctc ggatgataac gcctgccatt acagacttat cgattttgca attcagctta
    18481 actttgcgtg acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc
    18541 agtgcggcag cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga
    18601 ataaactgct ccagaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga
    18661 atcaggttct gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggcg
    18721 agcgtttctg gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc
    18781 tcggcggcaa acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag
    18841 tcaaaagctg ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc
    18901 ctccttacag ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg
    18961 aacgttcgat gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct
    19021 cttcacgggc acgtttacgc tcggcttcaa tttctgcctg cgcctgggcc acgattttag
    19081 tacgttcctg ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg
    19141 cctgctcgat gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg
    19201 cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt
    19261 ctttttgacg tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca
    19321 gaacgaacag gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac
    19381 aatgcctcta tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac
    19441 gcgacagcga acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc
    19501 agacccataa cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct
    19561 gcgccttcca ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc
    19621 agacccatca tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca
    19681 gtctccagtt tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc
    19741 catcgacaga tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag
    19801 gatgtggaaa atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc
    19861 agcaatcaga atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccaag
    19921 tgaaactggt ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc
    19981 ccagtgattg aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt
    20041 gatgctgtag aacagaatca ggataaatac gcccaatgcc atagacagcg ttacgttcac
    20101 gtccgcagac ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg
    20161 cagcaggtcg ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt
    20221 cagggccagc ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt
    20281 aacaaagccg atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc
    20341 ctttttggct acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa
    20401 catggagtca atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga
    20461 gaatgtacgc aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt
    20521 catattttct gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt
    20581 atctgaacca ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa
    20641 accgccaacg ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcgccaaat
    20701 gcgaatgtcc aggccacccg gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat
    20761 atcataaaca aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc
    20821 cagaaggggt ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg
    20881 agcagaagct tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact
    20941 cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa
    21001 gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca
    21061 aaaggtgaat aaatgtttaa atatttttcc agtgcataca attgcgactt ttctgctaac
    21121 cctgttcgat catgaaaaac tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca
    21181 gatcacatat tgcgcatgtt cgcgcacagc atatttattt acttggcaaa tgatgccttt
    21241 gcaagtttat gatatttcag tctaaaaaca gatactgttt taataaatga catttacaca
    21301 acaaaaacca cccattgaca tttttaataa tgttttaaca gccaatgatg gttcttagcg
    21361 ccgattttta gcagactgat attttcacta atgacttatt ttctgcttac caaaaaaagc
    21421 cacgttatct tgttgatgca aaagagtgaa cgtggcgtta aatgtaacca gttatatcag
    21481 tagaaaacct ggttgttgtt aacagtctaa ccggtcaatt ttttatgatt tttttgataa
    21541 aaattaaatt ttatttgctt taatcaccac cagatgacgt tcgccatcca gggctggaac
    21601 ctgaagttta accactgatt cgacctgata ttcttcgggc aacaaagcga tttcatcttc
    21661 cggcatttgc cctttcagcg cgtagaaacg gccttgctca ccaggaagat ggtggcacca
    21721 gctcaccata tcgttcagag aggcaaaagc gcggctaatt acgccatcaa atggcggctc
    21781 tgaaggaaac tcttctaccc tgctctgtac tggttcaata ttctccagtt taagctcatg
    21841 ttgcacctga cgaaggaaac gcacgcgttt accaaggcta tccaacagag tgaaatgggc
    21901 ttcaggacgc acgatagaga gtggaatgcc tggcagtcct ggtccggtgc cgacatcgat
    21961 aaaccgttca ccttgcagat acggtgccac cacaatgcta tcgagaatat ggcgtaccag
    22021 catctcatta ggatcgcgga ccgaagtcag gttgtacgct ttgttccatt tatgcagcat
    22081 attcacgtag gcaataagct ggtttttctg gtgatcggta agcgaaatac ctgcgtcttt
    22141 cagcagtaag gagagtttgt tgagcacggt gattacctgt tcttgatgcg ttgcctggta
    22201 agcgggtgct taccaggcat ttttaatgcg ttatgcgcta cgacgcagca taccctgttt
    22261 tttcagccac accagcagaa tggagatggc cgcaggcgtg acgccagaaa tacgcgaagc
    22321 ctggccgata gaggctggtt tgtgatcgtt aagtttggcg atcacttcgt tagaaagacc
    22381 ggatacctgg cggtaatcca gtgtcgcggg tagcagggtg ttctcgttac gcagctgctt
    22441 ttcgatctca tcttgctggc gcgcgatata accttcgtat ttaacctgaa tctcaacctg
    22501 ttccgccgcc tgttcgtctg tcaatgcagg ggcaaacggc gtcagcgtgg ttaatttttc
    22561 ataagtcatt tccggacgac gcagcagatc ttcaccactg gcttcacggg aaagcggcgc
    22621 agtcaggtga gcattcactt cggctgcagc ttccgccgac ggggttaccc aggtcgattt
    22681 cagacgctga cgctcacgct cgatattctc aagtttctcg ttaaagcgcg cccaacgttc
    22741 gtcatccact aggcccagtt cacgaccgat ttcagtcaaa cgcagatccg cattatcttc
    22801 gcgtagcatc agacgatatt ctgcgcgcga agtaaacata cgatacggtt ctttggttcc
    22861 taaagtgcac aggtcatcaa ctagtacgcc gagatacgcc tgagaacgtg ccggagccca
    22921 accttctttg tcagcagaca gacgggcagc gttaagaccg gccagcaaac cttgcgcagc
    22981 ggcttcttcg taaccggtag tgccgttaat ctgaccagca aagaacagcc cctggataaa
    23041 cttgctctcc agcgtcggtt tcaggtcgcg aggatcgaag aagtcatact caatggcata
    23101 acccggacgc acgatcttcg cgttttccat cccctgcata gagcggacga tttgcatctg
    23161 cacatcgaac ggcaggctgg tggagatacc gttcggataa atttcattag aggtcagtcc
    23221 ttccggttca aggaagatct gatgctgatt tctgtcggcg aagcgcatga ctttgtcttc
    23281 gatcgacggg cagtagcgtg ggccgacacc ttcgatcacc cctgcataca ttgggctacg
    23341 atcgaggtta ctgcggatca catcatgggt tttctcgttg gtatgagtga tataacacgg
    23401 cacctgctgg ggatgctggg acgcattgcc cataaacgag aataccggca ttgggttatc
    23461 gccatgctgt tgcgccagca cactaaagtc gatagttcga gcatcaatac gcggtggtgt
    23521 cccggttttc agacgaccaa cgcgcagcgg cagttcacgc aaacggcgag aaagcggaat
    23581 ggacggcgga tcaccagcac ggccaccgct gtaattatcc agaccgatat gaattttacc
    23641 gtcgaggaac gtcccaacgg tgagcacgac ggctttggca cggaacttca gtcccatttg
    23701 ggtaacagca ccgaccacgc gatcgttttc gacaataaga tcttcaaccg cctgctggaa
    23761 gatcatcagg ttcggttggt tctccagcgc cgtacgtacc gcctgacggt agagcacacg
    23821 atccgcctga gctcgggtag cgcgaaccgc cggtcctttg cttgcgttta gtatcctaaa
    23881 ctggataccc gcttgatcga tcgctttcgc catcagaccg ccgagtgcat ccacttcttt
    23941 taccagatgt cccttcccaa taccgccgat cgccgggttg cagctcatct gccccagagt
    24001 gtcgatattg tgtgtcaaaa gcagagtctg ttgacccata cgcgccgcgg ccatcgcggc
    24061 ctcggtgcct gcatgacccc cgccaatgat gatgacgtca aaaggatccg gataaaacat
    24121 ggtgattgcc tcgcataacg cggtatgaaa atggattgaa gcccgggccg tggattctac
    24181 tcaactttgt cggcttgaga aagacctggg atcctgggta ttaaaaagaa gatctattta
    24241 tttagagatc tgttctattg tgatctctta ttaggatcgc actgccctgt ggataacaag
    24301 gatccggctt ttaagatcaa caacctggaa aggatcatta actgtgaatg atcggtgat
//
LOCUS       DAFAVF010000056        24190 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFAVF010000056 DAFAVF010000000
VERSION     DAFAVF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.056"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            40..501
                     /locus_tag="LGK42_002251"
     CDS             40..501
                     /locus_tag="LGK42_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HBK2658494.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(566..1327)
                     /gene="pphC"
                     /locus_tag="LGK42_002252"
     CDS             complement(566..1327)
                     /gene="pphC"
                     /locus_tag="LGK42_002252"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBK2658495.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(1324..1983)
                     /locus_tag="LGK42_002253"
     CDS             complement(1324..1983)
                     /locus_tag="LGK42_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK2658496.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2204..2263)
                     /gene="ibsA"
                     /locus_tag="LGK42_002254"
     CDS             complement(2204..2263)
                     /gene="ibsA"
                     /locus_tag="LGK42_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBK2658497.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(2536..2592)
                     /locus_tag="LGK42_002255"
     CDS             complement(2536..2592)
                     /locus_tag="LGK42_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBK2658498.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            2871..4118
                     /gene="mdtA"
                     /locus_tag="LGK42_002256"
     CDS             2871..4118
                     /gene="mdtA"
                     /locus_tag="LGK42_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBK2658499.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            4118..7240
                     /gene="mdtB"
                     /locus_tag="LGK42_002257"
     CDS             4118..7240
                     /gene="mdtB"
                     /locus_tag="LGK42_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBK2658500.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPELMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            7241..10318
                     /gene="mdtC"
                     /locus_tag="LGK42_002258"
     CDS             7241..10318
                     /gene="mdtC"
                     /locus_tag="LGK42_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBK2658501.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVVVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            10319..11734
                     /locus_tag="LGK42_002259"
     CDS             10319..11734
                     /locus_tag="LGK42_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658502.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFALSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVHVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            11731..13134
                     /gene="baeS"
                     /locus_tag="LGK42_002260"
     CDS             11731..13134
                     /gene="baeS"
                     /locus_tag="LGK42_002260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBK2658503.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            13131..13853
                     /gene="baeR"
                     /locus_tag="LGK42_002261"
     CDS             13131..13853
                     /gene="baeR"
                     /locus_tag="LGK42_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBK2658504.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            14044..14376
                     /locus_tag="LGK42_002262"
     CDS             14044..14376
                     /locus_tag="LGK42_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBK2658505.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            14585..14881
                     /locus_tag="LGK42_002263"
     CDS             14585..14881
                     /locus_tag="LGK42_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBK2658506.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            14883..15179
                     /locus_tag="LGK42_002264"
     CDS             14883..15179
                     /locus_tag="LGK42_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2658507.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            15282..16643
                     /gene="yegQ"
                     /locus_tag="LGK42_002265"
     CDS             15282..16643
                     /gene="yegQ"
                     /locus_tag="LGK42_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBK2658508.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(16916..17134)
                     /gene="ogrK"
                     /locus_tag="LGK42_002266"
     CDS             complement(16916..17134)
                     /gene="ogrK"
                     /locus_tag="LGK42_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HBK2658509.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(17216..18379)
                     /locus_tag="LGK42_002267"
     CDS             complement(17216..18379)
                     /locus_tag="LGK42_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBK2658510.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNKLEASVAPSLDGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(18379..18858)
                     /locus_tag="LGK42_002268"
     CDS             complement(18379..18858)
                     /locus_tag="LGK42_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2658511.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVNETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(18873..21320)
                     /locus_tag="LGK42_002269"
     CDS             complement(18873..21320)
                     /locus_tag="LGK42_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2658512.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVRINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTNAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(21313..21432)
                     /locus_tag="LGK42_002270"
     CDS             complement(21313..21432)
                     /locus_tag="LGK42_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBK2658513.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(21465..21740)
                     /locus_tag="LGK42_002271"
     CDS             complement(21465..21740)
                     /locus_tag="LGK42_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBK2658514.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(21797..22315)
                     /locus_tag="LGK42_002272"
     CDS             complement(21797..22315)
                     /locus_tag="LGK42_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBK2658515.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(22328..23518)
                     /locus_tag="LGK42_002273"
     CDS             complement(22328..23518)
                     /locus_tag="LGK42_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBK2658516.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(23805..>24190)
                     /locus_tag="LGK42_002274"
     CDS             complement(23805..>24190)
                     /locus_tag="LGK42_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HBK2658517.1"
                     /translation="IEVKKPFDDKILRKTPYRDNYIPTSELSGGIMQAEKYIFHLSKW
                     GVKGEKELTNAYKNSLPAGMRIRISNPKAIIIVGRDQIANGNMTDGQLLDFEIIKRKY
                     ANMIDILTYDDLLRRLNNTIEALKG"
BASE COUNT         5398 a         6584 c         6411 g         5797 t
ORIGIN      
        1 ttttgctaac tttgactaca aagggattct ggagaaaaaa tgaaaaagat attacttatg
       61 ttgagtctgc tattttttac taccgcgggc tttagtgaag tttcggatac gttagtgacg
      121 ggaggttatg ataaacaagc gatgtctgac gcgattaaac atgcgcggaa agaaaccgat
      181 aaattcatcg aggttatgaa taaaaaagat gccgatactt tcgcagtcaa agccccgata
      241 accgatcacg gaagaaccga gcatttctgg cttacggatg tcacttattc taacggcatg
      301 tttattggcg taatcagcaa tgaccctggt attgttacaa acgtagaata tggccaggag
      361 tggaaaatca agaaagaaga tatttcagac tggatgtata cgcgcgggga taaaatttac
      421 ggcggttata ccattgatcc tttattggtc acctatccaa aagaagaagc cgacgagctt
      481 agagcaaaat tagttcgcta atcagccgtt atatttattg tttaaactgg cggttgaatt
      541 gttgtcttca accgccatga gaaactcact ctgcccacag cgccagtgct aacgttttat
      601 catcatcggt acgctcgttt accgctgggc tggacaaaaa ctgctttaac aattcaggta
      661 ataaatcgag ttgttcctgc gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag
      721 gcacatgcgg agaattatcc aacatattta acgccagtcg ctggatacca tccgtaaatg
      781 ctgcaacttt atgcacacgc tcagtgctgg tgaaagtttc cagtctggaa acggcatctt
      841 catcggtaat gaagtgcgtc atattggcat attcacccac catcggcgta agcggcaatt
      901 gcagaccgtg accaaaatcc accaccacgc cgccatcgcc aatctgcata atcaaggtgc
      961 cattagccga tgaaatcaat cccagaaacg tgcaggcaaa atcgcgaacc gctaattctt
     1021 tggcctctgc ctcggcaaaa agtcgctgac gaatagtcag cacaatatcc gtggcgagga
     1081 tatcattaag ccccaattcc ccaccctgca ctttttgcga catataagcc atcgcttcat
     1141 tgacggcgag catcgcgcct tcgccaccct gcgagacgct gcctgcacca tcagcaagaa
     1201 acatcaccag taatggttgc tgatcgttta accaggcaac ctgcatctgg caggcatcct
     1261 gacaaggtaa atcagcgcta atgtgcgacg ttccaacagc ggatgcataa accagacgcc
     1321 agctcacact gacgtccagc cttttggcgc ttccagcaca acttccgttc ccggcgtgga
     1381 gcgggaaacc gaacgcagcg aactggataa ccagctaaac agttcacgga attgtaatcc
     1441 ttgcagaggc aaaggctgac gaacgctgat ttgtgccagc gtcttcatat ccgccccctg
     1501 aacagcgatg gaaaagaagg caaacttctt atcttcttcc ccctgaaaca ctttgttggc
     1561 ggcagcctgc cactcatcgg ttggagcgcc atcggtgatc aggaaaatcc acgggcgata
     1621 atacgaaata ccatttgcgc ggtactcacg tttcctctct tcaaccatat ccagggcttt
     1681 ggtaatggcg gcccccattg gcgtatcccc ctgggcaaac aggatgggcg ggaaaaagtt
     1741 agcggcgctg gtaaagggtt gttccacatg caccgggccg aacgtcacaa taccgagttc
     1801 cactcttttt aaggccagcg aatcggcaag cagctcatcg cgaaaggtaa ccaatccggc
     1861 gttaagttca ttaatgggtc tgccgctcat cgagccagaa acatccagca gcaaaatgca
     1921 ggggcaacgt ggttccgggt tactggcaaa atcgctggtg gcaaacgtga tttgttcgct
     1981 cataagaaat cctttcttag agaattatcg aggtccggta tcataccgac tgcaataatg
     2041 aaataagagc aaacgaagaa actttcagaa ttaggcccgg cggcgcagaa aattttgcta
     2101 ttcgcctcgc aagaacggag agccgtcgat tgacactaag ggcggagtga cataatttca
     2161 ggagtgaggg ttagggagag gtttccccct ccccctggtg ttcttagtaa gcctggaagc
     2221 taatcactaa gagtatcacc agtatgatga cgtgcttcat cataaccctt tccttattaa
     2281 aagccctctt ctccgggaga ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta
     2341 tctcctttcg ccatgcactt agtctatcgc tgacttgcag ataaaatagt ttccagtgtg
     2401 attattgcgt aacgtctcgc aaagaatttt tgctggcagg atcgcagacg gcaaagcctg
     2461 cggattgaca atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc
     2521 cccgccctgg tagtcttagt aagcggggaa gcttatgact aagagcacca cgatgatgag
     2581 tagcttcatc atgacccttt ccttatttaa ggccccttcc tcgggagggg ctttcccgtt
     2641 tcagcatccg gctgaaatct gcggcttacc tcctttcgcc ctgtatgtag tctatcgcta
     2701 acgcgtagat aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgc
     2761 cattcagacg attcccgata gtgtttcata attcctccat ttttctccct tattggctgg
     2821 ctacactagt atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca
     2881 gttataaatc ccgttgggta atcgtaatcg tggtggttat cgccgccatc gccgcattct
     2941 ggttctggca aggccgcaat gactcccgga gtgcagcccc aggggcgacg aaacaagcgc
     3001 agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg cccattagcc ccggttcagg
     3061 cggcgaccgc cgtagaacag gcagttccgc gttacctcac cgggcttggc accattaccg
     3121 ccgctaatac tgttacggtg cgcagccgcg tggacggtca actgatggcg ttacatttcc
     3181 aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga aattgacccc agccagttca
     3241 aagttgcatt agcacaagcc cagggccaac tggcaaaaga taaagccacg cttgctaacg
     3301 cccgccgtga tctggcgcgt tatcagcaac tggcaaaaac caatctcgtc tcccgtcagg
     3361 agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg caccattaag gctgatgaag
     3421 caagcgtcgc cagcgcgcag ctgcaactcg actggagccg catcaccgca ccagtcgatg
     3481 gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat ctccagtggt gataccaccg
     3541 gaattgtggt gatcacccag acgcatccta tcgatttagt ctttaccctg ccggaaagcg
     3601 atatcgctac tgtagtgcag gcgcaaaaag ccggaaaacc gctggtggta gaagcctggg
     3661 atcgcaccaa ttcgaagaaa ttaagtgaag gcacgctgtt aagcctcgat aaccaaatcg
     3721 atgccacaac cggtacgatt aaagtgaaag cacgctttaa taatcaggat gatgcgctgt
     3781 ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga
     3841 tcccaacagc cgccctgcaa atgggcaatg aaggccattt tgtctgggtg ctgaatagcg
     3901 aaaacaaggt cagcaaacat ctggtgacgc cgggcattca ggacagtcag aaagtggtga
     3961 tccgtgcagg tatttctgcg ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg
     4021 aaggggcgaa agtggaagtg gtggaagccc agagcgccac cactccggaa gagaaagcca
     4081 ccagccgcga atacgcgaaa aaaggagcac gctcctgatg caggtgttac ccccgagcag
     4141 cacaggcggc ccgtcgcgcc tttttattat gcgtcctgtc gccaccacgc tgctgatggt
     4201 ggcgatctta ctcgccggga ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga
     4261 agtggactat ccgaccattc aggtggtcac gctctaccca ggtgccagcc cggatgtcat
     4321 gacctctgcc gttaccgcgc cgctagaacg ccagttcggg cagatgtctg gcctgaaaca
     4381 gatgtcgtcg caaagttccg gcggtgcgtc agttatcact ttgcagttcc agctaacatt
     4441 accgctcgat gtcgccgagc aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt
     4501 gccgagcgat ctgcctaacc cgccggttta cagcaaagtg aacccggcag atccgccgat
     4561 catgacgctc gccgtcacct caaccgccat gccgatgacg caagtggaag atatggtgga
     4621 aacccgcgtc gcgcagaaaa tctcgcagat ttccggtgtc ggactggtga cgctttccgg
     4681 cggtcagcgt ccggctgttc gcgtcaaact taacgctcag gcgattgccg ccctcggcct
     4741 gaccagcgaa accgtgcgca ccgccattac cggcgctaac gttaactcgg caaaaggtag
     4801 cctcgacggc ccttcccgtg cggttacgct ttccgctaac gaccagatgc aatccgccga
     4861 agagtatcgc cagctaatca tcgcctacca gaacggcgcg ccgattcgtc ttggcgatgt
     4921 cgcaaccgta gagcaaggtg cagaaaacag ctggcttggc gcatgggcga acaaagaaca
     4981 ggccattgtg atgaatgttc agcgccagcc cggtgctaac attatctcca ccgccgacag
     5041 catccggcag atgctgccac agctcaccga gagtctgccg aaatcggtga aggtaacagt
     5101 gctttccgat cgcaccacca atatccgcgc atcggtcaac gatacccagt ttgaattgat
     5161 gatggctatc gcgctggtag tcatgattat ctacctgttt ttgcgcaata ttccggcgac
     5221 catcattccc ggcttcgcag tgccgctgtc gttaatcggc accttcgcgg tcatggtgtt
     5281 tctcgatttt tcaatcaata acctgacgct gatggcgtta actatcgcca ccgggttcgt
     5341 ggtcgatgac gccatcgtgg tgatcgaaaa tatttcccgc tatatcgaaa aaggcgaaaa
     5401 accgttggcg gcggcgctca agggcgcagg tgaaatcggc tttaccatta tctcgctgac
     5461 cttctcgctg attgcggtgt tgatcccact gctgtttatg ggcgatatcg tcgggcgact
     5521 gttccgcgaa tttgctatta ccctggcggt agcgattttg atctcagcgg tggtgtcgct
     5581 gaccctgaca ccgatgatgt gcgcgcggat gctcagccag gagtcgttgc gtaaacagaa
     5641 ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg
     5701 actggcgaaa gtgctgaatc atccgtggct gaccttaagc gtggcactca gcacgctgct
     5761 gcttagcgtg ctgctgtggg tgttcattcc gaaaggtttc ttcccggtac aggacaacgg
     5821 cattattcag ggcactttgc aggcaccgca atccagctcc tttgccaata tggcccagcg
     5881 gcaacgccag gtcgcggacg tgattttgca ggatccggca gtgcaaagtc tgacctcatt
     5941 tgttggcgtt gatggcacta acccgtcgct gaacagtgcg cgtttgcaga tcaacctcaa
     6001 accgctggat gaacgtgatg atcgggtgca aaaagtcatc gcccgtctgc aaacggcggt
     6061 ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg caggacctga ccatcgatac
     6121 tcaggtcagc cgcacccagt accagtttac cttgcaggcc acctcactgg atgcgctcag
     6181 tacctgggtg ccagagttga tggaaaaact ccagcaactg ccacagcttt ctgatgtctc
     6241 cagcgactgg caggacaaag ggctggtggc gtatgtcaat gttgatcgcg acagcgccag
     6301 ccgtctgggg atcagcatgg cggatgtcga taacgccctg tacaacgcgt ttggtcagcg
     6361 gctgatttcc actatttata ctcaggctaa ccagtatcgc gtggtgctgg aacacaacac
     6421 cgaaaatacc ccaggcctcg cggcgctgga taccattcgc ctgaccagca gcgacggtgg
     6481 cgtggtgccg ctaagctcaa ttgccaaaat tgagcagcgt tttgcgccgc tctccatcaa
     6541 ccatctggat cagttcccgg taacgaccat ctcctttaac gtgccggata actattcgct
     6601 gggtgatgcg gtgcaggcga ttatggacac cgagaagacg ctgaatctgc cggtggatat
     6661 caccacgcaa ttccagggca gcaccctcgc cttccagtcg gcgctgggca gcactgtctg
     6721 gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc ggcattctgt acgagagctt
     6781 tattcacccg atcaccattc tctcgacgct acccaccgca ggggtcggcg cactgctggc
     6841 attaatgatt gccggaagtg agctggatgt gattgcgatt atcggcatta ttttgctgat
     6901 cggtatcgtg aagaagaacg ccatcatgat gatcgacttc gcgctggctg ctgagcgcga
     6961 gcaaggcatg tcgccgcgcg atgccatcta ccaggcttgt ctgttgcgtt ttcgtccgat
     7021 cctgatgacc accctggcgg ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt
     7081 cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc ggcggtctga ttgtcagcca
     7141 ggtgctgacg ctgttcacca cgccggtgat ttatctgctg ttcgaccgcc tggcattgtg
     7201 gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa gtgaagtttt ttgccctctt
     7261 catttaccgc ccggtggcga cgattttact gtcggtcgcc attaccctct gcggcatact
     7321 gggcttccgt atgctgccgg tcgccccgct gccgcaggtc gattttccgg tgattatggt
     7381 cagcgcctcg ctgcccggtg cgtcgccgga gacaatggcg tcttccgttg ccacgccgct
     7441 ggagcgctca cttgggcgca ttgccggggt tagtgaaatg acctccagta gttcgctcgg
     7501 cagcacgcgg attattttgc agtttgattt tgaccgggat atcaacggcg cagcgcgtga
     7561 tgtgcaggcg gcgatcaacg ctgcacaaag tttgctgccc agtgggatgc ccagccgtcc
     7621 gacctatcgc aaagcgaacc cgtcggatgc gccaattatg atcctcacgc tgacatccga
     7681 tacttattct cagggtgaac tgtacgattt cgcctcgacg cagctggctc cgacgatttc
     7741 gcaaatcgac ggtgttggtg atgtcgatgt cggcggcagc tcactgcccg ccgtacgcgt
     7801 cgggctgaat ccgcaggcgc tgtttaatca gggcgtctcg ctggacgacg tacgcaccgc
     7861 catcagcaat gccaacgtgc gtaaaccaca gggggcactg gaagatggca ctcaccgctg
     7921 gcagatccag accaatgatg agctaaaaac cgccgctgaa tatcagccgt tgattattca
     7981 ttacaacaac ggcggcgcgg ttcgtctggg tgatgtggcg acggtgactg actcggtgca
     8041 ggatgtgcgc aacgccggga tgaccaacgc caaaccagct attttgctga tgatccgcaa
     8101 gctgccggaa gccaatatta tccagacggt tgacagcatc cgggcaaaat taccggagtt
     8161 gcaggagacc attccggcgg cgattgatct gcaaattgcc caggatcgct cccccaccat
     8221 tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc tcggtggcgc tggtgattct
     8281 ggtggtgttt ttattcctgc gctcgggtcg cgccactatt attcccgccg ttgtggtgcc
     8341 ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc ggattcagtc tcaataacct
     8401 gtcgttaatg gcgctcacca tcgctactgg tttcgtggtg gatgacgcca tcgtggtgct
     8461 ggaaaacatt gcccgtcatc tggaagcggg gatgaaacca ctacaggccg cactgcaagg
     8521 tactcgcgaa gtcggtttta cggtgctgtc gatgagtctg tcactggtgg cggtgttcct
     8581 gccgctgctg ttgatgggcg gattgccggg ccgactgtta cgcgagtttg ccgtgacgct
     8641 ttctgtcgcc attggtattt cgttgctggt ttccctgacg ctaacgccga tgatgtgtgg
     8701 ctggatgctg aaagccagca agccgcgtga gcaaaagcga ctgcgtggtt ttggtcgcat
     8761 gttggtagcc ctgcaacaag gctacggcaa gtcgctgaaa tgggtgctca atcatacccg
     8821 tctggtgggc gtggtgctgc ttggcaccat tgcgctgaat atctggctat atatctcgat
     8881 cccgaaaacc ttcttcccgg agcaggacac tggcgtgttg atgggcggga tacaggcgga
     8941 tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag gatttcatga aaattatccg
     9001 tgacgatccg gcagtggata atgtcaccgg atttaccggc ggttcgcgag tgaacagcgg
     9061 gatgatgttt atcaccctca agccacgcga cgaacgcagc gaaacggcgc agcaaattat
     9121 cgaccgtctg cgcgtgaagc tggcgaaaga accgggggcg aatctgttcc tgatggcggt
     9181 acaggatatt cgcgttggcg gacgtcagtc gaacgccagc tatcagtaca cgttgttatc
     9241 cgacgacctg gcggcactgc gcgaatggga gccgaaaatc cgcaaaaaac tggcgacgtt
     9301 gccggaactg gcggacgtga actccgatca gcaggataac ggcgcggaga tgaatctggt
     9361 ttacgaccgc gacaccatgg cacggctggg aatcgacgta caagccgcca acagtctgtt
     9421 aaataacgcc ttcggtcagc ggcaaatctc gaccatttac cagccgatga accagtataa
     9481 agtggtgatg gaagtggatc cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt
     9541 cgttatcaat aacgaaggca aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc
     9601 gaatgcccca ctatcggtga atcatcaggg attatcggcg gcctcgacca tttcgtttaa
     9661 cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg atcgatcgcg caatgaccca
     9721 gcttggggtg ccttcgacgg tgcgcggcag ttttgccggc acggcgcagg tgttccagga
     9781 gacgatgaac tcgcaggtga tcctgattat cgccgccatc gccacggtgt atatcgtgct
     9841 gggtatcctt tacgagagtt acgtacatcc gctgacgatc ctctccaccc tgccctcggc
     9901 gggcgttgga gcgctgctgg cgctggagct gttcaatgcc ccgttcagcc taatcgccct
     9961 gatagggatc atgctattaa tcggcatcgt gaagaaaaac gccattatga tggtcgattt
    10021 tgcgcttgaa gcccaacggc acggtaacct gacgccgcag gaagctattt tccaggcctg
    10081 tctgctgcgt tttcgcccga ttatgatgac taccctggcg gcgctgtttg gtgcgctgcc
    10141 gctggtattg tcgggcggcg acggctcgga gctgcggcaa cccctgggga tcaccattgt
    10201 cggcggactg gtaatgagcc agctccttac gctgtatacc acgccggtgg tgtatctctt
    10261 tttcgaccgt ctgcggctgc gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat
    10321 gacagatctt cccgacagca cccgttggca attgtggatt gtggctttcg gcttctttat
    10381 gcagtcgctg gacaccacca tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg
    10441 ggaaagtccg ttgcatatgc acatggtcat tgtctcttat gtgctgaccg tggcggtgat
    10501 gctgcccgcc agcggctggc tggcggacaa agtcggcgtg cgcaatattt tctttaccgc
    10561 catcgtgctg tttactctcg gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact
    10621 gttgctggca cgcgcgttac agggcgttgg cggcgcgatg atggtgccgg tcggcagatt
    10681 gacggtgatg aaaatcgtac cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt
    10741 acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc ggtctgctgg tggagtacgc
    10801 atcgtggcac tggatctttt tgatcaacat tccggtgggg attatcggtg cgatcgccac
    10861 attgctgtta atgccaaact acaccatgca gacgcggcgc tttgctctct ccggattttt
    10921 attgctggcg gttggcatgg cggtattaac cctggcgctg gacggcagta aaggtacagg
    10981 tttatcgccg ctgacgattg caggcctggt cgcagttggc gtggtggcac tggtgcttta
    11041 tctgctgcac gccagaaata acaaccgtgc cctgttcagt ctgaaactgt tccgtactcg
    11101 taccttttcg ctgggcctgg cggggagctt tgccggacgt attggcagtg gcatgttgcc
    11161 ctttatgaca ccggttttcc tgcaaattgg cctcggtttc tcgccgtttc atgccggact
    11221 gatgatgatc ccgatggtgc ttggcagcat gggaatgaag cgaattgtgg tacatgtggt
    11281 gaatcgcttt ggttatcgtc gggtactggt agcgaccacg ctgggtctgt cgctggtcac
    11341 cctgttgttt atgactaccg ccctgctggg ctggtactac gttttgccgt tcgtcctgtt
    11401 tttacaaggg atggtcaact cgacgcgttt ctcctccatg aacaccctga cgctgaaaga
    11461 tctcccggac aatctggcga gcagcggcaa cagcctgctg tcgatgatta tgcaattgtc
    11521 gatgagtatc ggcgtcacta tcgccgggct gttgctggga ctttttggtt cacagcatgt
    11581 cagcgtcgac agcggcacca cacaaaccgt ctttatgtac acctggctta gcatggcgtt
    11641 gatcatcgcc cttccggcgt tcatctttgc cagagtgccg aacgatacgc atcaaaatgt
    11701 agctatttcg cggcgaaaaa ggagcgcgca atgaagttct ggcgacccgg tattaccggc
    11761 aaactgtttc tggcgatttt cgccacctgc attgtcttgc tgatcagtat gcactgggcg
    11821 gtgcgtatca gttttgagcg tggctttatt gattacatca agcatggtaa tgaacagcga
    11881 ttacaactgt taagtgatgc gcttggcgag cagtatgcgc agcatggcaa ctggcgcttc
    11941 ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt catttgaaca cgataattcg
    12001 gaagataaac ccggcccggg tatgccaccg cacggctggc gtacccagtt ctgggtggtt
    12061 gatcaaaaca acaaagtgct ggttggtccg cgagcgccga ttccacctga cggtacacgg
    12121 cgacccattc tggtcaacgg tgcggaagtt ggcgcggtga tcgcctcccc cgttgagcgg
    12181 ttaacgcgca atactgatat caatttcgat aaacaacagc ggcaaaccag ctggttgatt
    12241 gtcgctctgg caacgttact cgcggcactc gccacttttc tgctggcgcg cggtttgctg
    12301 gcaccggtaa aacgacttgt cgatggcacg cacaaactgg cggcgggcga tttcactacc
    12361 cgcgtaacgc ccaccagtga agatgaactg ggcaagctgg cgcaagactt caaccagctt
    12421 gccagcacgc tggagaaaaa ccagcaaatg cggcgcgatt ttatggccga tatttctcac
    12481 gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg
    12541 cgtaaattca cgccggagac ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa
    12601 ctggttgacg atctccatca gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa
    12661 gcaccggtag atttgatccc actgctggaa gtggcgggcg gcgcatttcg cgaacgattc
    12721 gccagtcgtg gcctgaaact gcaattttcc ctgccagaca gtattaccgt atttggcgat
    12781 cgcgaccgtt taatgcagtt attcaataac ttactggaaa acagcctgcg ctacactgac
    12841 agcggcggca gcctgcaaat ctctgccggt cagcgcgaca aaacggtgcg cctgaccttt
    12901 gccgacagtg cgccaggtgt cagtgacgat cagctacaaa aattgtttga acgtttttat
    12961 cgcaccgaag gttcccgcaa ccgtgccagc ggcggttccg ggctggggct ggcgatttgc
    13021 ctgaacattg ttgaagcaca taatggtcgc attattgctg cccattcgcc ttttggcggg
    13081 gtaagcatta cagtagagtt accgctggaa cgggatttac agagagaagt atgaccgagt
    13141 taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga agatgaaccg aagctggggc
    13201 agttgctcat tgattatctg cgtgctgcga gctatgcgcc gacgcttatc agccacggcg
    13261 atcaggtact gccgtatgta cgccagacac caccggatct gatcctgtta gatctgatgc
    13321 tccctggcac cgatggcctg acgctgtgcc gggaaattcg tcgtttttct gacattccga
    13381 tcgtgatggt gacggcaaaa atcgaagaga tcgatcgcct gctggggctg gagattggcg
    13441 cagatgatta tatctgtaag ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca
    13501 ttttgcgccg ttgcaaaccg cagcgcgagt tgcagcaaca ggatgctgaa agcccgttga
    13561 ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg
    13621 cggaatttcg tctgctgaaa acgctctctc atgaaccagg aaaagtgttc tcccgcgagc
    13681 atttgctcaa tcatctttat gacgactacc gcgtagtaac cgaccgcacc atcgacagcc
    13741 acattaaaaa cctgcgccgc aagctggaat ctctcgacgc cgaacagtca tttatccgcg
    13801 ccgtttacgg cgttggttac cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc
    13861 gacatgactt tgttagccgg agatggcgtt cagaccagat ccggcaacat tatcccacgc
    13921 atggtcagca aactgacatt atctctccgc cctggcaggc atcagaaaat cgctcatact
    13981 ttaatcggta aacagcacct ttagatgctg ttttcgatac acaatttcaa tcaaggagtc
    14041 attatggctg gttggtttga actcagtaag agcagtgata atcagttccg gtttgtgcta
    14101 aaagcgggca atggtgagac tatcctcacc agcgagctgt atacctcaaa agcctctgcg
    14161 gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa
    14221 acggcaagta acggcaaatt ctatttcaat ctgaaagccg ctaatcatca aattatcggc
    14281 tccagccaga tgtacgccac cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc
    14341 aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc gtgggttgtt
    14401 ggcgtccggc tttatatcca aataacgctt agttattaat aattaagagt aaccgccgcc
    14461 attaaaatag tgggcactga cgagcactcg ttgtgctttc tttgtttacc atcccatact
    14521 tatcctcctg aacatgttca tgtgccaaaa tatcttctaa tactttcgtg acgctatccg
    14581 gtttatgaca tacactgatg taagattttt acttacaggc gggcgtaaaa tggccagaac
    14641 gatgacggtt gatctcggcg atgagttgcg tgaatttatc gagtccttaa ttgaatccgg
    14701 cgactatcgc acccaaagcg aagtgatccg tgagtcactg cgattacttc gagaaaagca
    14761 agcagagtct cgcctgcaag cgttacgtga catgctcgca gaagggttga gtagtggtga
    14821 agctcagccg tgggaaaagg acgcgttctt acggaaggtg aaagcaggga taaggaaata
    14881 atgtgcgtat tattaaactc atgccaaaag ccaatgagga tttagaaggc atctggtatt
    14941 acagctatca ccatttcggc gaaccacaag ccgacagata tgtggaacat ctttcagacg
    15001 ttctccagat tctgagtaac aataatatag gtacgcccag acctgaatta ggcgaaggta
    15061 tttttgttct tccttttgaa cgccacgtca tttatttcct gcaatcacca ggggaaatta
    15121 tcgtcattcg tatccttaac caaaaccagg acgccaccag acatttacac tggagctaaa
    15181 cccgccgatt gcgctacaat gcccgccctt aaagtggggg cactccccta accgcttcat
    15241 caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa ccggaactcc
    15301 tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc gcagatgctg
    15361 tttatgcggg ccagccgcgt tattccctgc gtgtgcgcaa caacgaattc aaccacgaaa
    15421 atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat gtcgtggtca
    15481 acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg
    15541 aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg gtgcgtgagc
    15601 acttccctga aatgccgatc cacctttcgg tgcaggctaa cgccgtgaac tgggcgacgg
    15661 tgaaattctg gcagcaaatg ggcctgaccc gcgtgatcct ctctcgcgag ctgtcgctgg
    15721 aagagattga agagatccgc aatcaggtgc cggatatgga gatcgagatc ttcgttcacg
    15781 gcgcgctgtg catggcctac tccggtcgct gcctgctctc tggctatatc aacaagcgcg
    15841 acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc caggaaggga
    15901 aagaagatga tgttggcaac atcgtacaca agtacgagcc gattccggtg caaaatgttg
    15961 agccgacgct gggtatcggc gcaccaaccg acaaagtgtt tatgatcgaa gaggcccagc
    16021 gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc atgaactcga
    16081 aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg cattcgctga
    16141 aaatcgaagg tcgtaccaaa tctttctact attgtgcacg caccgcacag gtttaccgca
    16201 aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg gaaactctgg
    16261 aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact cacgacgatt
    16321 atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt ggtgagttta
    16381 ccggtgagcg caagggggac ctcgcggcgg tagcggtgaa aaataaattc tccgttggcg
    16441 acagccttga gctgatgacg ccgcaaggca acattaattt tacccttgag cacatggaaa
    16501 acgccaaagg cgaagctatg ccgatagcac caggcgatgg ttatactgtg tggctcccgg
    16561 tcccgcagga tcttgagctc aattacgcgc tgctgatgcg taatttctcc ggggaaacca
    16621 cgcgtaatcc ccacggtaag tgattaattt cgattatttt tcccggatgg aaaattctta
    16681 gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct gaaaaacata
    16741 acccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat ctcccttaca
    16801 cgggcttatt ttttacgcgt aatacaatga aataaaagga tttatttctg gtcacgtcca
    16861 cacattgacc acatcgacaa aaaagcccct cgactgaggg gcttcctgtt tgtaattaca
    16921 tccacataat ttgctgccct gacggcaacg ggtgcggcct cacggcgtgg acttctcccg
    16981 gcttcacgat gtatctctgt accgactcat aagtgatgaa cgtggcgctg caattcacgt
    17041 tctgacactg gtgataacgc tcttttgtcg tgtcagtgat atagcgactt gtacgcgcat
    17101 gtgcggcatg ctggcataaa ggacaatgaa acatcgcgag cacctcttcc ggttttgttg
    17161 atggtgccat tttagttaat ttacccttat aaaacaaaca gataaaaaca aaacatcact
    17221 catcatcttc tgtttcgtac tccacatcag aaagcctgac ctcaagctcc aaggacgtcg
    17281 tgaagccgct gttattcaga aaatgtgtca ccttagtgat tgtccagtcc tgctcgtcta
    17341 tgacgcgctt aaagcctgac actttaaccg gtgtttccgt gtaaatatct gcccgaccgg
    17401 tagccaggct gatggagaac tccgctacgc cccgttgcag tttatcccac ttcgcctgag
    17461 cggcgcgcat ggcctgcgct ttcgtggcat ataccgtggt cagggcaaaa acgttgtcag
    17521 cctcaccggc catgtattca ccttcgcgcg cttccggtac ttttggcgct ttcttctgcg
    17581 tgaccggttt cgctttcggg tgctccagtg cgcgcaggtg tttttctttc tttttgcgtt
    17641 tcagttttac cttctgcttt tgcggcttcg ggtctttggt gtgtaaccac tttgccgtta
    17701 cgccggtgta agctccacgg tcagcaatcg caaaatgatg acggtcgccg tcgctgcggg
    17761 tgatggtaat ctgcgggatt tttttaccgc tggccgtcac cccctgcccc gctttgagaa
    17821 acagcagttt tcccattttt accgacacct caccgccgtt gcgttcagca aggcgggtca
    17881 ggaatttcgc atcagactcc tgcgactggt cgatgtgcgg gattttaatt ccgtccagtg
    17941 acggcgcgac actggcttcc agcttgttac gggaggctat cgcctcaaca atcgcaccga
    18001 gcgtggtgtc atgccatgag ccttcacggc gggaattgag cgtcccgcga aaatctgcac
    18061 tccgggcgcg gatggtaacc acatccggcg cgccccggtg ttcaacctca tcaacggtga
    18121 atttcccttt gcataccagg gcaaaacctt tccagccgat atacaccgtc aggacagcgc
    18181 cacgaaccgg cagcccgacc tgcccgtcgg catcgttcag ttcaatatca agctggtcag
    18241 cctcaaagcc ccggttatcc gtcagggtca tgctcatcag acggtcgctg atattgccgg
    18301 taatatccct gctgtcgagc atcagcatgt aatccggcgt cagcgtactg cctgcatcaa
    18361 atgtcagcgc atccagcatt atcccgcccc cgtcataccc gtgaatttag tcgccatact
    18421 gccagcctta ccgatgagcg attccgcctg tttaccgata tcgccataaa gcgcggccag
    18481 tgattcatca acgcgggtga gcgacagcgt aaaatcaatt ttccggggtg tgccgtctgc
    18541 aaagaaaata ctccctgttt cattcaccct gctgatgaca tacatgccgt aaatcatgcc
    18601 ggtgccatcc agcaacggcc acgcccggcc ttcctctgcc atcagcctga gcgtggtcat
    18661 cgtcagcttg ccgccggtca gttcgggata aagcacgccg gcaagcgtga tgttttcctc
    18721 acccacaccg agaaactggt aggcatcccg tttaccgata cgggaatttg acggccagcg
    18781 ataatctgat tcacgctgca tggtctggtg tggcagcgtc tggcgcataa aaacaaacat
    18841 acctaacgcg agcatcattt ttcgtcacct ccttaaccgt catgcatcat gctggcacgg
    18901 gcgcgcgcac gtttatcccg ctcgtatttt tcgagcgcat cctgtaactg gcggtcaagc
    18961 tgtgtccccg gcgcagtacc accggtcagg ctgatgtgat attcgttttt actctggtcc
    19021 acataagagc ggccagccgg tgccgtgacc ggctgataag cctgatagcc tgcataagag
    19081 ctggtcgccg gaatataacc accggtgcca tacgtggcgg cattagtcct tgcggcggtc
    19141 tggtcaagtg tgtctgactc tttgttgatg acaccgagtt tttccagtac ccagtcaata
    19201 ccactgcgca gtttgttgaa cgcattaagc ggcagcatca gcgcgtcagc cagtgcctgc
    19261 ccgaacatga cgcccgtgtc acggcaacgg ttcagggtgt cctgggtggc tttgaccggg
    19321 gcaatcaggt ttttaaacca ctgccacgcg gcctgtaact tttcacccag ccagtcaaac
    19381 accggtttaa gtggcgtgaa cagttccccc accggcgcaa atgccgcttt cagcccttcc
    19441 accacaccgc caaagaatgc gctgacaggc tcccagtatt tacggataag caacgccccg
    19501 gcgacaatgg cggccaccac ggccacaacc ggccagctaa tcgccccgat ggccgtcata
    19561 acagcactgc caaccgtcgt gaagattgcc cccattgcgc ctgctgccgc gatgatggca
    19621 ttgatgccgg tgataaccgg ccaggctacg aggccaatgg caccaatgat gccagtcagc
    19681 gccagtgcac caccggcaat aatgccgatg gttgacgcca gtgatttgtt tttctggatc
    19741 cagccgtcga gttttaacac atactttgtg gccgtctgcg tgagcttacg cagtgcgcct
    19801 tcctgctggt caaacaggtc agtccccacc gcctcataag cggactgaaa ctccttaaag
    19861 tcaccgccga ggttgtcctg catgatattt accagctcgg cagtcttccc gtctgaggct
    19921 ttaaacgcag cggtcagttt gtccagcttt ccagttgagg cggcagtcat cagcacggcg
    19981 gcggctgagc tggcctcctc cccgaaaata gttttcatgt attcagcctg ctgggcagta
    20041 ccgagccggt ttttctcaaa actggcctgc atttctttca gaatggtaaa tactggtcgg
    20101 gtattcccct tactgtctga ggttttcacg ccaagctctt tgagtgcatc ccatgctttt
    20161 cccgtcggtg cctgcagacg gcttaacacg gcacggcttc ccgtccccgc cattgagcct
    20221 gtgatttttg catcatgcag cgccccgacc attgcggcgg tttcttcaat gctgacaccg
    20281 gcattttttg ccacaggtgc ggcataggtc agcgcatcgc tcatgccgtc aaaatcggcg
    20341 gcggttttgt tcatcgtcat ggagagaaca tccccgatat gagcgacctt atcgtttgaa
    20401 agctgaaagg cggatttcat ccccatcagc agggcggcgt tttcttccat cgtgcggcgg
    20461 ttcgccagcg ccatattcag cgtgaccggc gttgccgcct gaatggcatc aacatcccca
    20521 cccgctttcg cgatgattat ctgtgcaccg gccgcatcat ccgctgaggc ggcggtattg
    20581 tcgccgagct ggcgcgcctg tttgcgtagt gcggtcattt cggcggagtc ttttgccact
    20641 ccgagcacgg cctgcaattc tgagtttttc tgcgcaaact cataaccggg catcagcaac
    20701 ttaactccgg ccatcgttcc cgccgccgca atccccacac cggcagcgcc cactgaggcc
    20761 atatttccgg ccagttcctt gcctgcctga taacgctgtt ttactgcgtt aagttttgcc
    20821 tgttgcgcac tgacacgcgc cagcgcgtca cgctgacggt taagctgtgc ggtggtttca
    20881 ctgatacggt ttttcagtcc ctgctcatca tgtgcaagat tgcgggtatt aattctcaca
    20941 acggccagtt cccgctgctg gcgtttaacg gaatccgtca ggcggttata tttcgcctgt
    21001 aagtcctccg ccgcacgctt tgcggattcc agcactttcg cctgagcacg tgtcggacgt
    21061 tcggtgtttt taaactgtgt ggcaagggct tcggcctcct gccgtgcctt ttcaagtgca
    21121 tgaccagtca cggcgagctg tgcactggtc ttgcgaaatc cctcaatacg ggatgcctga
    21181 ccgttcagct cgcgcagtga tttttgtgtt tcccggatat cccccgacag cgatttgctc
    21241 gctgtgcgga tggatttaaa cgggcgggat gcctggtcaa cagccctgag caatacctgt
    21301 aattttacat tgttactcat tcgtgtttcc gcttcgccgg agcgcctttt cgcgccatgt
    21361 gatgagttcg gtcaggctca tgggatacag ttctgatggc ggccagtgaa atatcactgc
    21421 cacatccgcc atcaggtcat cgaccgagag atttttcgaa aacgtcactg caccgagttc
    21481 ggagacaaaa aaccgaccac cttaccggcc agcgccacaa ggtcaggcag ttccagcgcg
    21541 gcgacctcct gctcggtcag catcggtgcc gtcatgcgcg gcagcacctt aatcagtgca
    21601 tcgacttcgg agtttgcgac cgcagccaga ctgacaccgc gcagcgtccc ggcattaggt
    21661 ttcatcagcg tgacctgttc gataacctgc tcaccacgct tgaccggatt gtccagggta
    21721 atcacatttt ctttgttcat ggttttctca cttctgaatc ggggttaacc ggtcagccag
    21781 gctgaccgga tgaaaatcac aggccgatat tgcggcggtg ttgctccagc cggtcgacgc
    21841 cgttcacctt ctcaatcatg ttgatggtgt cgatttcgac cagctcctta ccgtccatcg
    21901 tcagccggaa ataggtgcag accacggaga ttttcgactc ggtgtcttct ccctgtttgc
    21961 cctcgccggt gtcgatttct ttctgacgtc cacgcatgac cacctcgacg gccaccgttt
    22021 cgccggtatc gtcgcgctgg taagagcctg caaaacgaat cggcacggca tccacaccgg
    22081 ttgcggcgta aagctcccag ataaccgaat ccgggaagcc accgagcgac cactccattg
    22141 acagcgcatc gtcatcaaga ccgaggtcta ccggtgcgct gccgttcatc cccgcaccgc
    22201 gatagttttc gagcttacgg gtcagttttg gcagcgtgac ggactttgca acgccctgat
    22261 agctgtagcc gttcagaaag acgttcatta acttgagttt gcgcggcatt gccatcggtc
    22321 aggctcctta attgctgtta accgaggtga ccagatttgc caggtattta tcggtaatac
    22381 gctggcgcag ggtcaggttt tcaagaggag gaaccggggt atagtcgtag tcgatataca
    22441 gttttccggc cttaagggtt tccgcatcat tggattcttc gctgaaccag caggtcgcat
    22501 ccacgatata gccgtttgtt ttcagctcac gaaatttggc attgatgccg tcaacgatgt
    22561 cgcgaatcag cgttgcggtg atgggcttgt ccaccgccca catgtgcgcc tcagccatcg
    22621 tgtcggccag cacctgcgcg gtgcgggtgt agttttcaaa gaggaacagc gggtcatcgg
    22681 agcaggtacg gttaccccag aagcggaaac cgtcacggcg aatcagcgta gtgacgcctg
    22741 actcgttaag caggtcagca tcggtgccgg actcctgcaa atcccagaag acggatgcgc
    22801 tgatgccggt aacaccgttc accccgacat tggacagcgt tttatgccag ccctgctcct
    22861 ggtcgatttt agcgcgcaga cccagcgcac gggcggtggc atacgcggtg gcggtggtac
    22921 tggtgaccgt atcccatgcg aggaaatccg gccagatgac catcagctca cgctggctga
    22981 aattctggcg gtaggctttc acctcggaaa tggtcttaca gccccatgcg ctgatatacc
    23041 cgaaagcacg cagcttctga cagactgatg ccagtgcaac agctacctct ttggtgtcca
    23101 gtcccggcac gccgagaata cgcggtttaa caccggttac cgactccgcc gccagcaggg
    23161 ctttcagtcc ggtgtactga ccgttttcgt ctgtggtgcc gatgatattg gaaacggtct
    23221 gcgcgagttt cgtttcctcg tcgtcgccgg tgccgtcttc cacgcgcaca acaacggtga
    23281 ccggttttga ctggtcagcg atggcctgta acgacgccgc cagcgtgcct tttttaccgg
    23341 cctttgcaat tgcgctctgc acattggtaa tcagtaccgg tttattgagg gggaaggttt
    23401 cggcatccgc atcgctggcc gtacagacca tgccgacaat ggccgtggat acggtggaaa
    23461 tgacgcgggt gccgtcgtta atctccagca cctgcacgcc gtgatgatag tcactcatcc
    23521 gtttaactcc gtggttaatg ggtgcaacta ttttctgttg ggcagtgcat aagacgctat
    23581 ttgacctgac tggtcagtgg atgaaacaac agattaaaga aaaggcgggc aactcgcccg
    23641 cccgtcctga tttgtactca ctcatttttc aactgacaat ttacatagcc caaaagctat
    23701 caaatcagac agtctgcttt gagccaaaag cagacgttat ggtatgtcaa tcaaacaaga
    23761 ggaagtgacc tgctccccgt tgtgggtgtg aacctcatgg gcattcaacc tttcaatgct
    23821 tcaatcgtat tattaagtcg acgcaacagg tcgtcgtacg tcaatatgtc tatcatgttg
    23881 gcgtacttac gtttgattat ctcaaaatca agcaactgac cgtcggtcat gttaccgttc
    23941 gctatctgat cgcgcccgac aatgatgatg gcttttgggt tagatatacg aatacgcatt
    24001 cccgctggca gagaattctt gtaggcattc gtaagctctt tttcaccctt aacaccccat
    24061 ttagataaat gaaaaatata cttttcagcc tgcataatac cgccgctaag ttctgatgta
    24121 ggaatatagt tatcgcgata gggcgtcttg cgaaggatct tatcatcaaa cggctttttg
    24181 acttcgatga
//
LOCUS       DAFAVF010000057        24089 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFAVF010000057 DAFAVF010000000
VERSION     DAFAVF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.057"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            125..1624
                     /gene="pitB"
                     /locus_tag="LGK42_002275"
     CDS             125..1624
                     /gene="pitB"
                     /locus_tag="LGK42_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBK2658518.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1673..2365)
                     /locus_tag="LGK42_002276"
     CDS             complement(1673..2365)
                     /locus_tag="LGK42_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBK2658519.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2539..3252
                     /locus_tag="LGK42_002277"
     CDS             2539..3252
                     /locus_tag="LGK42_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBK2658520.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3284..4042
                     /locus_tag="LGK42_002278"
     CDS             3284..4042
                     /locus_tag="LGK42_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658521.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4088..5437
                     /locus_tag="LGK42_002279"
     CDS             4088..5437
                     /locus_tag="LGK42_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBK2658522.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5437..6261
                     /locus_tag="LGK42_002280"
     CDS             5437..6261
                     /locus_tag="LGK42_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658523.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6273..6833
                     /locus_tag="LGK42_002281"
     CDS             6273..6833
                     /locus_tag="LGK42_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBK2658524.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWKSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6864..7934
                     /gene="lptF"
                     /locus_tag="LGK42_002282"
     CDS             6864..7934
                     /gene="lptF"
                     /locus_tag="LGK42_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBK2658525.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7931..9010
                     /gene="lptG"
                     /locus_tag="LGK42_002283"
     CDS             7931..9010
                     /gene="lptG"
                     /locus_tag="LGK42_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBK2658526.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSVRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(9045..10217)
                     /locus_tag="LGK42_002284"
     CDS             complement(9045..10217)
                     /locus_tag="LGK42_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK2658527.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10217..10465)
                     /locus_tag="LGK42_002285"
     CDS             complement(10217..10465)
                     /locus_tag="LGK42_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK2658528.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10497..11411)
                     /locus_tag="LGK42_002286"
     CDS             complement(10497..11411)
                     /locus_tag="LGK42_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK2658529.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11408..13138)
                     /locus_tag="LGK42_002287"
     CDS             complement(11408..13138)
                     /locus_tag="LGK42_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBK2658530.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            13500..14642
                     /locus_tag="LGK42_002288"
     CDS             13500..14642
                     /locus_tag="LGK42_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2658531.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(14649..15413)
                     /gene="glcC"
                     /locus_tag="LGK42_002289"
     CDS             complement(14649..15413)
                     /gene="glcC"
                     /locus_tag="LGK42_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBK2658532.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            15664..17163
                     /gene="glcD"
                     /locus_tag="LGK42_002290"
     CDS             15664..17163
                     /gene="glcD"
                     /locus_tag="LGK42_002290"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBK2658533.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            17163..18215
                     /gene="glcE"
                     /locus_tag="LGK42_002291"
     CDS             17163..18215
                     /gene="glcE"
                     /locus_tag="LGK42_002291"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBK2658534.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18226..19449
                     /gene="glcF"
                     /locus_tag="LGK42_002292"
     CDS             18226..19449
                     /gene="glcF"
                     /locus_tag="LGK42_002292"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBK2658535.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLERARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            19454..19858
                     /locus_tag="LGK42_002293"
     CDS             19454..19858
                     /locus_tag="LGK42_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBK2658536.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            19880..22051
                     /gene="glcB"
                     /locus_tag="LGK42_002294"
     CDS             19880..22051
                     /gene="glcB"
                     /locus_tag="LGK42_002294"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBK2658537.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQLERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22407..24089
                     /gene="glcA"
                     /locus_tag="LGK42_002295"
     CDS             22407..24089
                     /gene="glcA"
                     /locus_tag="LGK42_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBK2658538.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         5715 a         5981 c         6482 g         5911 t
ORIGIN      
        1 attgtctgat acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg
       61 cccatgccgt taaaatcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta
      121 atatatgcta aatttatttg ttggccttga tatatacaca gggcttttgt tattgcttgc
      181 tctggcattt gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt
      241 ggcaaccgtt atttatactc gtgccatgca accacaactt gctgtggtga tggcggcatt
      301 ttttaacttt tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat
      361 gttaccaacc gatttgttgc tgaatatggg gtcaacccac ggtctggcga tggtcttttc
      421 catgctgctg gcggcgatta tatggaacct ggggacgtgg ttcttcggtt taccggcctc
      481 cagttcgcac accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac
      541 cggctcatcg gtgatggatg cgttaaacct gcgtgaagtg accaaaattt tctcctcgct
      601 gattgtttcc cctatcgtcg gcctggtcat tgcgggaggc ctgatattcc tgctgcgacg
      661 ctactggagc gggacgaaaa agcgtgatcg tattcaccgc attccggaag atcgcaaaaa
      721 gaaaaaaggc aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc
      781 gggcgtggcg ttttcgcacg gcgcgaacga cgggcagaaa gggatcggcc tggtgatgct
      841 ggtactggtg gggattgccc ctgctggctt cgtcgtcaat atgaacgcgt ccggctatga
      901 aattacccgt actcgcgatg ccgtctccaa cttcgaacac tatctgcaac agcatcctga
      961 actgccgcag aagttgattg cgatggaacc tccattgcct gcggcatcga ctgatggcgc
     1021 gcaagtaaca gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa
     1081 aacgatgctg ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca
     1141 gctgcgccgc attatgctgt gcatctcaga tacctccgcg aagctggcga aactgccagg
     1201 cgtcagtaaa gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat
     1261 tgagtacgct ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat
     1321 gattggctgg cgtcgtgtag caatgaccat cggtgagaag attggtaagc gcggcatgac
     1381 gtatgcgcaa ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc ttgccagtta
     1441 tattgggatg cccgtctcta caacacacgt actctcatct gccgtagcag ggacgatggt
     1501 ggtggacggc ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg catgggtgtt
     1561 tactttaccg gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat
     1621 ttaaccttcc tgaaaatgcc cggtcctggc gatcgggcat ttccattttt gactagtgat
     1681 aaccacgcgc ggtcataaaa tccgtaatcg ctttttctgc atcaaccaac acctgttcca
     1741 gtggctgatt ggcatcgata tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta
     1801 tggctatctt tcttgccagc gattcccttt tgtggtcagg cttacgcgcg caggccacgt
     1861 ctagatcgac attgagtttg atgaccagat caggcttatg gctcgccatc cagtgaaacg
     1921 ctttacgttc ctggcttgcc agccatgaga caaaacgacc accttcaacg ttaggtggga
     1981 acaccgtacc atcgtaagcg ccaggaattt ggtcctgagg ataacggtcg gttagaacaa
     2041 ttaacccgcg acgacgacag gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa
     2101 acgctgtaat taccagcgcc ggaactggtc ctggcaattt ttttgctgtt ttcacctgat
     2161 ttcgttcaat tgttttatgt aaggattttc ccatcaacgg taattttgtc actgcacgac
     2221 cgacatttcc ggcttgtttt cctaaatgaa ctctttcggc agcaccatat ttttcgacaa
     2281 cggtaatgag atgttcacac accgttgact tgcctgaacc atcgctacca ataacggcaa
     2341 ttaatggagg tgttattgac tgcatgaata tatccttaaa tttaaatatc catccaaaat
     2401 atttatttgg ttaatatgtt tttatgaaag cgtaattcag gtcaatgtca caattaacca
     2461 ttgtcacaat aaggttgaac ggatattctg agcacggacc acgtaatatt caatacatta
     2521 tttactgccg ggtttttcgt gaatcaacgt aatatgtcaa taattaattc cacgccagtg
     2581 cgtgttattg ccattgtagg atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg
     2641 gtaaatgaac tggcagcaag aatgccaaca gaacacattt atctcgggca atcgtccggg
     2701 cgaattggcg aatggatttc acagctccct gttattggcg caccttgtgg gcgttatctg
     2761 cgaagtaaag cggcacatgt gcacgaaaaa ccctcaacac cgcctggcaa tattactgcg
     2821 ctggttatct atctgctttc ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa
     2881 agccagcaag gctttctgct catcaccgac cgctacccgc aggttgaagt gccgggtttt
     2941 cgctttgatg gcccgcaatt ggcaaaaacc acgggcggta acggttggat aaaaatgtta
     3001 aggcagcgcg agctgaagct gtaccaatgg atggcatctt atttgcccgt attgttgatt
     3061 cgtcttggca ttgatgaaca aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg
     3121 ttacaggaaa aaatcgccgt tacgccgcaa ctgacattca atggcgcaaa gatccttgaa
     3181 ctggatgggc gacaccccgc cgatgaaatt ctgcaagcgt cactacgcgc aattcacgcc
     3241 gcgctttcct gatcaccaaa atggataggg gatgatttaa cgaatggatg cactacaaac
     3301 tcaaactgtt aatagtacaa ccgcgccgca gcccaactac attccggggc tgattgcggt
     3361 ggtcgggtgt gatggcaccg gtaaatccac actgaccacc gacctggtga aatcgctgca
     3421 acaacactgg caaaccgagc ggcgctatct ggggctgctc tccggcgaag acggcgacaa
     3481 aatcaaacga ttgccgttgg ttggcgtctg gctggaacgg cgactggcgg ccaaatcctc
     3541 gaaaacccaa agcatgaaaa ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg
     3601 cttctcgctg cgaagaatgg cgaatctacg caaggttcag cgactggcgc aaagtggcgt
     3661 tctggtggtc agcgatcgct tcccgcaggc tgaaatttcg ggcttttatt atgatggacc
     3721 ggggattggc gtcgaacgtg cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg
     3781 gcgtttatac caacaaatgg cgcaatatcg cccggaatta attattcgcc tgggcattga
     3841 tattgagact gccatctccc gcaagcctga ccatgactat gccgagctgc aggacaaaat
     3901 cggcgtcatg tcgacgattg gctataacgg cacaaaaatt cttgagattg attccagagc
     3961 gccctacagc gaagtgctgg aacaggcaca gaaggcggtt tctctggttg ccatcgtttc
     4021 tgaccgccga agtttaactt aaggctggcg ggatactttt tactatcaac gataaggtca
     4081 ggctgaattg gcgggtttta acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat
     4141 tattcgcaat agcgcctggt taggctccag taatgtcgtg agcgccttgc tgggtctgtt
     4201 ggcgctctcg tgtgccggta aagggatgac gcccgccatg tttggcgtac tggtgattgt
     4261 gcaatcgtac gccaagtcga tcagcgattt tattaagttt cagacatggc aactggtggt
     4321 tcagtacgga acgccagcct tgaccaacaa taatccgcag caatttcgca atgtcgtctc
     4381 attttccttc tcgctggata tcgtcagcgg cgcggtggcg attgtcggcg gcattgcctt
     4441 actgccattc ctttcccatt cattaggtct ggatgaccaa agcttttggc tggcggcgct
     4501 gtattgcacg ctcattcctt caatggcttc ctccacgccg accggcattc tgcgtgcggt
     4561 agatcgcttc gatttaattg ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg
     4621 gagcgtcgta gcctggtatt ttgactttgg ttttgcgggt tttgttattg cctggtacgt
     4681 gtcgaatctg gttggcggca ccatgtactg gtggtttgca gcgcgcgaat tacgccgcag
     4741 aaatatccat aatgccttca aattgaatct gtttgagtct gcccgacaca tcaaaggcgc
     4801 gtggagtttt gtctggtcaa ccaacattgc ccactccatc tggtcggcgc gtaactcgtg
     4861 cagcacagtg ctggtgggga tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc
     4921 catgacattc ttcgacgccg ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc
     4981 agaggtgatg cgtttagatc cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg
     5041 tttactggcg ggcggaatcg gtattctggt ggcgctcgcg gtgtttatcg tcggcaagcc
     5101 gctcatttcg ctggtgtttg gcgttaagta tctcgaagcg tatgacctga ttcaggtgat
     5161 gctgggcgca attgtgattt cgatgctggg cttccctcag gagtcattgc tattgatggc
     5221 gggaaaacag cgcgcttttc tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt
     5281 gctgttcatg ttctgtcatc tgtttggtgt gctgggagca gcgtttgctt acttcggtgg
     5341 gcaatgtctg gacgtggcgc tctcgttaat tccaacctta aaggcatttt ttcagcgcca
     5401 ttccttgctt tataacgcag ccggagagaa atcctgatga tgagaaaata tttcccgctt
     5461 gaggcgagcg agcgtctatt tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt
     5521 agtttgccgc cgactattgc actaagttgt accaccgaaa tcattcatga taattacgcg
     5581 ttatgcctgc aattctggct caatggcgtt gatcgtcagg aattactgcg tttagttcgc
     5641 aaacaagcca aaggcgatga gctgactgct gatgagcgca aacaatttaa atacatgcga
     5701 gcgcgctaca agcacctgcg ttttgcgcaa cggctgtatt taaagaagca tcaggccgga
     5761 tttttattcg gcaaaacgac cgtttttctg gggcgttttc aggacggctt tcgtaacggt
     5821 aagaaaaata ttgtgtcgta ttacggcaac ctgttacgga tttatttaag ctctccggtc
     5881 tggtcgttgg tgaactattc attacgccat agccagttag aaagcgtaag cagttttatt
     5941 gcctatcggc aaaagcaaat gcataccctg aaagagatta ttgccaaacc gcgactaacc
     6001 ggcagggaat ttcatgatgt gcgaaaaatc atcagccaac aggtttctta ttacgacacc
     6061 ttaagatcgc ttgatcctga aaataaagag gcgttacaga tttcccgttt cctcgccgcc
     6121 attaatggcc tgatgggaga taaacacgac gacatggtgg cggatgatat ggaaaaccgc
     6181 cagtcgtatg atgcaccgtt ggcgctggat agcgatattc gtcagcgctt agagctgctg
     6241 atttcgcgtt ttccgctata gccggagcaa tgatgtcatc tcgtttgatt attgcgttga
     6301 ttatcatgct cttagccccg ggtgtgcagg cacacaattt cgtcactggc aaaacggtta
     6361 cccctgttta tatccaggaa ggcggcgagc tgcttctgaa tagcgacgac gaaattcact
     6421 accaaaagtg gaaaagcaca caattagcgg gaaaagtccg cattattcag tatatcgccg
     6481 ggcgtaagtc ggcgaagaaa aagaactcac tgttaattaa agcggttgaa gcggcaaact
     6541 ttccgcagga tcgttttcag cccaccacca ttgtgaacac cgatgatgcc atctttggta
     6601 ccggctattt tgtcgttggc aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat
     6661 tcgttattga cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa
     6721 ccattctggt gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc
     6781 cggaagaggt agatcatgtc attgcgttgg cgcagaaact gatcaatgaa taaaaaactc
     6841 aaaggattga gggcgttagt tagatgaagc tggttgaaca ctacatcatg cgtggtacgc
     6901 gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac
     6961 agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg
     7021 ttttctacaa agtactgatt gcactggaga tgttgttacc tgttggtctg tatgtgtcag
     7081 ttggcgtgac gctagggcag atgtataccg actcggaaat taccgctatc tctgcggcgg
     7141 ggggcagtcc gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt
     7201 ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg
     7261 agcagcagtc gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta
     7321 acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg
     7381 acgcgctgat ttatacttct actgccaatc gaacccgtat tttccgcgcc cgttcggttg
     7441 atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc
     7501 ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg caattacatc
     7561 tgaatccgct ggatcaaagc cctaacgtca aacgcaaagc aaaatcggtc acggagctgg
     7621 cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc cgtggcctga
     7681 ccgcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg cggcaagggc
     7741 gattttcaac gttattgcca ctgacgttgc tgtttattgc cattttttat ggcggcgacg
     7801 tctgccgtac gctggtggct aacggcgcga ttcccctcat tcctggtttg tggttagtac
     7861 ccggactcat gctaatgggc ctgctgatgc tggtcgcacg cgacttctct ttgctgcaga
     7921 aattttcccg atgaatgttt tcagtcgcta tttaatccgt catctctttc tcggttttgc
     7981 cgccgccgca gggctattgc tgccgctttt taccaccttc aacctgatta acgaactgga
     8041 tgatgtcagc ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc taatgacctt
     8101 accgcgcaca ctggtcgaac tttcgccgtt tatcgcgtta ttgggtggta ttgttggact
     8161 ggggcagtta tcgaaaaaca gtgagcttac cgccattcgc agcacggggt tttctatctt
     8221 ccgtattgca ctggtggcgc tggttgccgg gatattgtgg actgtttcgt taggcgcgat
     8281 agatgagtgg gtggcgtcgc cattgcagca acaggcgttg caaatcaaat cgaccgccac
     8341 cgcgttgggg gaggacgatg atattaccgg caatatgctg tgggccaggc gcggtaatga
     8401 atttgtgacg gtgaaatcgc tgaatgaaca gggccagcct gtgggcgtgg agatatttca
     8461 ttatcgcgac gatctctcgc tcgaatccta catttatgca cgcagtgcca ccattgaaga
     8521 cgacaaaacg tggattctgc atggtgtgaa tcataaaaaa tggctgaatg gcaaagaaac
     8581 gctggaaata ttagataatc ttgcctggca atcgacattc accagtatga atcttgaaga
     8641 actgtcgatg ccgggcaata gcttttccgt tcgtcagctc aaccattaca tcaattactt
     8701 gcaggaaacc ggacaaccga gcagtgagta tcgcctggca ttgtgggaaa agctggggca
     8761 tcccatttta accctggcga tgattttgct ggcagtgcct tttaccttta gcgccccgcg
     8821 ctcgccgggg atgggcagcc gtcttgctgt aggcgtcatc gttggcttac ttacctggat
     8881 aagctaccaa ataatggtca atctgggatt attatttgcg ctgagtgcgc ccgttgctgc
     8941 actcggttta cctatggcat ttgtgctggc ggcgttgatt ctggtgtatc tatttgatag
     9001 gcaacattaa acccgccgtg gcgcaacacc acggcgggct cacattactg cttcagagta
     9061 gcaaatgcct gaatgatggc atcgatttgc tcacgactgt gggcagcatt tacgctacag
     9121 cgcagcagcg tgatgcctgc aggcgcggca ggcggcagga taagattcac atacacgccc
     9181 agcgagataa gctcgcgcca gatgcgtaaa ccttcctctt tcgaaccgat tattactggc
     9241 actaccgggc taatatgtga acttaactca taacccagtt tttgcagacc gtcatacaga
     9301 tggttggcgt tatccatcag cttttgccgc agctctggtt gtgtggcaat agttcttaac
     9361 gacgagcgca cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat atacgggcgg
     9421 ctgccgtagc gcagcacttc catcgcttcc gaaccgacag caaaaccgcc aatcgaggcc
     9481 agacttttac tgaaagtacc aacgataata tccacatcat cttcaacgcc gacagcttca
     9541 gccagtccgc gccccgttgc gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa
     9601 taaccaccga gacgacgttt gatatcaaca atttccgcca gcggcgcaac gtcaccgagc
     9661 atgctgtaga tgccctcaac gatgataatc gcctctttcg cccgttcacc gaggcgaacc
     9721 atgcgccgtt ccagatcttt agcatcgtta tggcggaaac gaataatttc cgcgccaccg
     9781 agagaacagg cgtcataaat actggcgtgg ctgtcggcat ccagtaacac cacggcgtta
     9841 tggtcggcaa gtgcgctgat gacgcctaaa ttagcggtat aaccggttga aaaaacgata
     9901 gcggtagggc gattgaaaaa ctcggcaatc tctttctcta acgccaggtg cggcgcataa
     9961 ctgccgtttg ccatacgaga accggtggtg ccggtacctt gggccgctaa cgcggcctga
    10021 ccttcgctga tggcatcatg attaaaggtt aaaccgagat aattattggt cccggccaga
    10081 atcacttttt tattgccgat ccggccttcg gtggcggaat acacttcatc aatacaggtg
    10141 ccaaacggtg ttaacccgtt gtcactaaat tgcagtcgct cgccagcaag gcgcgcatat
    10201 ttatcgtata gccccattat ttattctcca gccatggaag tagagtttcc attaattgcg
    10261 caggggtttt gacatccagc agaatattaa taggaatgga aatatcgaat ctgtcttcca
    10321 gcatcattaa gagatccatc actttgatcg actccaggcc aagatcgttg acgagatcgc
    10381 tgtcgggctt aatttccaca ccgttttcga ccaaatcctg taaacaggaa aggatgtaat
    10441 ccattactat ttcacgattt accatagaaa cgttaacaca cctttaaaca agacaatcaa
    10501 aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt aatatcttca
    10561 gaaatacggt tattacttgc cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg
    10621 gtcagcatag gttctttacc tgccaggcga ctcaacgcgg tactgatatc cgcaaggcag
    10681 gtgagtaccg gcagaggaat accaaccatt cgcacggaac cgcaacggac gttggcggca
    10741 agttgctgta tgcgttgcca gtcatagccg ccagcgacgc catcgcataa ttcataggtt
    10801 tgcgtctgta cagtttcggc gcttaaccac tgacccactg cttgcgcaaa atcggtgacg
    10861 tgcaggaaag agagctgtgt atctggtgca ccaagtcgtg gcagcaggcc gcgcagcatc
    10921 cagtcaaaca gcggttttaa ctctttatcg ccgggaccat acacggctgt cgggcgaaaa
    10981 acgcccagcg taatttcgtc agccattgca gtcagccgtt gttcggcgat gtgtttggaa
    11041 tttgcgtacc aggagagctc gggatggcgc gccgccagcg aagagataaa cagaaaacgt
    11101 tggcaaaagc agctctcttt tgctgcttgc atcaggcgca ggctgccgtc aacgttacag
    11161 cgggtgaaaa tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac cactgcgctg
    11221 gctccggcaa ccagcttgct aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg
    11281 agattatcat tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgcgg
    11341 gcgagcaggt tatcgataat atatttaccg ataaacccgg tagcgcccgt caccgcgaca
    11401 gtttgattca tgccagggat tcctgcacat gaaggcttgc agcataagcc ttctgataac
    11461 gttttttcgc ttccgcacgg gcaggcttgc cggaggatgt tcggggaata ctgtggggcg
    11521 gcaacagatc gatagccgct gttacgccaa attcgctttg gatccgtgct gccagcgcgt
    11581 ggataagctg cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt
    11641 tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt tcttgttccg
    11701 caatatattc aatatcctgc ggccagatat tacggccacg aataataatc agatctttaa
    11761 tacgtccggt gacatacaga taaccgtcca gcagataacc gaggtcgccg gtgtctaacc
    11821 agcccgtcgc ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcaggc
    11881 tggggccgga gatgcaaata tggcctacca cacgttccgc gaccggcata cctgcttcat
    11941 tgcggatttc aatgccatgt tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg
    12001 cgcgtgtttc agcacccggc gcgacggctt tgccctgata ttctaggatg tcgcgatcca
    12061 cttcgttaac cacaacccca gagccttcat cagagaagct gacagccagc gcattttctg
    12121 ccagtccgta gcacggcatg aacgttttat cgtcaaagtt aacctgacgg aaacattcag
    12181 cgaattgatg gagttgttct gcggagatcg gctcagcacc aataccagcg acgcgccagc
    12241 aggaaagatc cagttcagcg agatcttttt cattcacgcg gcgttggcac aattcatagc
    12301 caaacggcgg cgcaacggaa acggtgccgc gatttttact gatcaattta agccattgca
    12361 aaggacgcat ggcaaaatcc tgagtgcgca aataatctac tgaaagttgc gtggcgacgg
    12421 gggtcaggag aaagccgacc agtcccatat catggtagaa aggcagccag gagacgcagc
    12481 ggtcgccagg gcgtaattta atgccatcgt ggcttatagc acgtagatta gccatcactt
    12541 cgcgatgggt gataatgacg ccacggggaa aacgggtgct gccggaggtg tactggaggt
    12601 aggcgatatc gttcggaact ggacgctgga gcgcaacatc ggcttccggt aatgccttaa
    12661 accaggcgtg gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg
    12721 gcaaccactc atcaccagtg ataatggctg cgggctggca acttgccagt aaaccctgga
    12781 gtttggcgct ccaggaatcc cgctgaccga cgcccattgg aatcgccaac gggacggcga
    12841 ctaagccagc atactgacag gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa
    12901 tcagcgccac gcgttcgcct tttttcaggt tcagcgataa caaccgcttc gcaccagctt
    12961 cggcacgcgt ttttaacgtc tgatattcca gttgatcttc aagttggcaa cgtctgtcat
    13021 aaaaattcat tccggcgcta ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa
    13081 aatcggcata gcgcataggt agggaatgcg taaagatttt attagacata tacaccacga
    13141 aaaaattgga tatcaaaaat aaaatgacaa taatgcggtg gattatatag gcataaaaga
    13201 taaatgagat agtattttca tcggtgtgaa aatgaacatt gatttaaata atgagtttaa
    13261 gttttttgtt tttattgtga atgatattta atttatgaat atattaagaa gaagccttgt
    13321 ccggtcgcct taaatcgaca aaaggcattg aggcgcttct gtgtagtctc taaccttatc
    13381 ataatcatta ataaatacta tttattcttt gtttgtattc attgggttga ttattaaggt
    13441 gtcgttcgtt gctgttgcaa cctgtttttc accgttaatt atttgttatc cacagagata
    13501 tggaatgcga tctgctgatg attaaaattg aaaaagtaat taataaaaat gaccttaagg
    13561 catttatcgc ttttccgtca tcactttatc ccgacgatcc aaactggata ccccctttat
    13621 tcatagaacg caacgagcat ttgtctgcga aaaatccggg gacggaccat atcatctggc
    13681 aggcgtgggt ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg caaatagata
    13741 ccttgcaccg tgagcgttac ggtgaagata ccggtcattt cggcatgatt gacgccattg
    13801 atgatccgca ggtttttgcc gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag
    13861 gtgcaagtaa gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc ggattactga
    13921 ttgaaggttt tgacacacca ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg
    13981 cgcatattga acaactgggt tatcacaaag gcattgattt actggcgtgg tggatgcagc
    14041 gaaccgatct cactttctct ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg
    14101 tgaccattcg ctgcatcaat cgtcagcggt ttgccgaaga gatgcagatc ctgcgtgaga
    14161 ttttcaactc tggctggcag cacaactggg gatttgtgcc gtttaccgaa catgaattcg
    14221 cgaccatggg cgatcaactt aaatatctgg tgccggacga tatgatctac atcgctgaga
    14281 ttgattctgc accctgcgcg tttattgtcg gcttgccgaa catcaacgag gcgattgccg
    14341 atctgaacgg atcgctcttt cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca
    14401 gcggtgtgcg aactgcgcga gtaccgctga tgggcgtgcg cgacgagtat cagttcagcc
    14461 gcatcggccc ggtgattgcg ctgttattga ttgaagcctt acgcgatccg tttgcccgcc
    14521 ggaagatcga tgcgctggag atgtcatgga tcctcgaaac caatacaggc atgaataaca
    14581 tgctggaacg cattggggcg gagccgtaca agcgttaccg attgtatgaa aagcagattt
    14641 aataccagct aactcaggtt catctccagc ggcaccgccg agcgaatcaa atggtggcct
    14701 tccagctcga tatcgtggag attctttttc acggtccgca catgatcgcg tgctgcgcgc
    14761 tgggcgacgt gcggcagccg ctgcaacacc gcgttgtaga tcctcgcatg ctggcgatcg
    14821 atctgctttt tttgctgtgg tcgatggtag agattattta cgctggcaaa cactgaatta
    14881 aacatcagat cggtcaatga ttgcagcgta aacaccagca cctgattgtg agaagcctga
    14941 caaatggcga gatggaaagc gtgatccaac tgcgcatgtt cgatcagcga aatctctttg
    15001 ttgttctcac tggcggcgag cattttttca taacagcggg ttatcacaac aaaatcagcc
    15061 tgcgttccca gcgttgccgc cagccttgcc gattcgccct ccagtaatgc gcgaacgtcg
    15121 agcagatcgt acagcgttcg cggctgcgta ctgaacagat ggatcagcgg gctggtgtcc
    15181 tgcacccgat taagccgtgc gacacgagaa tcgcgaccct gcgccgtttc aataatcccg
    15241 cgcccgcgca gcacggtcag cccttcacgc agtgcggagc gtgagaagcc gagcttttca
    15301 cacagtcgac gttccgaggg aagtggctga ccgaccttca gtacgccgtc gataattaac
    15361 cgttcgatac tctctgcaac cacttcgcaa atagggcgac gttcatcttt cattcccgga
    15421 cctcgtgcac aggtaggacc aatttttctg ttcttaactc gcaaaacacg cacatcacgt
    15481 aagtgattat gttacatcaa tttaacattg agttaaccaa ggcaaggtca cagagctgga
    15541 aaaaaaatgg tctgaccggt agctaaagag atagacgaaa acgaaaagcc cgcttaataa
    15601 ctgttcacag aagcagcgcg caaaaatcag ctgccacaca acacaacaaa gcgaagccta
    15661 ctcatgagca tcttgtacga agagcgtctt gatggcgctt tacccgatgt cgaccgcaca
    15721 tcggtactga tggcactgcg tgagcatgtc cctggacttg agatcctgca taccgatgag
    15781 gagatcattc cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc attactggtt
    15841 gttctgccta agcaaatgga acaggtgaca gcgattcttg ctgtctgcca tcgcctgcgt
    15901 gtaccggtgg tgacccgtgg tgcaggcacc gggctttctg gtggcgcgct gccgctggaa
    15961 aaaggtgtgt tgttggtgat ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt
    16021 cgccgcgcgc gcgtgcagcc aggcgtgcgt aacctggcga tctcccaggc cgttgcaccg
    16081 cataatctct actacgcacc ggacccttcc tcacaaatcg cctgttccat tggcggcaac
    16141 gtggcagaaa atgccggcgg cgtccactgc ctgaaatatg gtctgaccgt acataacctg
    16201 ctgaaaattg aagtgcaaac gctggacggc gaggcgctga cgctgggatc ggacgcgctg
    16261 gattcacctg gttttgacct gctggcgctg ttcaccggat cggaaggtat gctcggcgtg
    16321 accaccgaag tgacggtaaa actgctgccg aagccgcccg tggcgcgggt tctgttagcc
    16381 agctttgact cggtagaaaa agccggactt gcagttggtg acatcatcgc caatggcatt
    16441 atccccggcg ggctggagat gatggataac ctgtcgatcc gcgcggcgga agattttatt
    16501 catgccggtt atcccgtcga cgccgaagca attttgttat gcgagctgga cggcgtggag
    16561 tctgacgtac aggaagactg cgagcgggtt aacgacatct tgttgaaagc gggcgcgact
    16621 gacgtccgtc tggcacagga cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat
    16681 gcgttcccgg cggtaggacg tatctccccg gattactact gcatggatgg caccatcccg
    16741 cgtcgcgccc tgcctggcgt actggaaggc attgcccgtt tatcgcagca atatgattta
    16801 cgtgttgcca acgtctttca tgccggagat ggcaacatgc acccgttaat ccttttcgat
    16861 gccaacgaac ccggtgaatt tgcccgcgcg gaagagctgg gtgggaagat cctcgaactc
    16921 tgcgttgaag ttggcggcag catcagtggg gaacatggca tcgggcgaga aaaaatcaat
    16981 caaatgtgcg cccagttcaa cagcgatgaa atcacgacct tccatgcggt caaggcggcg
    17041 tttgaccccg atggtttgct gaaccctggg aaaaacattc ccacgctaca ccgctgtgct
    17101 gaatttggtg ccatgcatgt gcatcacggt catttacctt tccctgaact ggagcgtttc
    17161 tgatgctacg cgagtgtgat tacagccagg cgctgctgga gcaggtgaat caggcaatta
    17221 gcgataaaac gccgctggtg attcagggca gcaatagcaa agccttttta ggtcgccctg
    17281 tcaccgggca aacgctggat gtgcgttgtc atcgcggcat tgttaattac gacccgaccg
    17341 agctggtgat aaccgcgcgt gccggaacgc cgctggtggc gattgaagcg gcgctggaga
    17401 gcgccgggca aatgcttccc tgcgagccgc cgcattatgg tgaagaagcc acctggggtg
    17461 ggatggtcgc ctgcgggctg gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt
    17521 ttgtcctcgg cacgcgcatc attaccggcg ctggaaaaca tctgcgtttt ggtggcgaag
    17581 tgatgaaaaa cgttgccgga tacgatctct cacggttaat ggccggaagc tacggttgtc
    17641 ttggcgtgct cactgaaatc tcgatgaaag tgttaccgcg accgcgcgcc tccctgagcc
    17701 tgcgtcggga aatcagcctg caagaagcca tgaacgaaat cgcccagtgg caactccagc
    17761 cgctacccat tagtggctta tgttacttcg acaatgcgtt gtggatccgc cttgagggcg
    17821 gcgaaggatc ggtaaaagcg gcgcgtgaac tgctgggggg cgaagaggtt gcgggtcagt
    17881 tctggcagca attgcgtgaa caacaactgc cgttcttctc gttaccaggt accttatggc
    17941 gcatttcatt acccagcgat gcgccgatga tggatttacc cggcgagcaa ctgatcgact
    18001 ggggcggagc gttacgctgg ctgaaatcga cagccgagga caatcaaatc catcgcatcg
    18061 cccgcaacgc tggcggtcat gcgacccgct ttagtgccgg agatggtggc tttgccccgc
    18121 tatcggctcc tttattccgc tatcaccagc agcttaaaca gcagctcgac ccttgcggcg
    18181 tgtttaaccc cggtcgcatg tacgcggaac tttgaggagc aggctatgca aacccaatta
    18241 actgaagaga tgcggcagaa cgcgcgcgcg ctggaagccg acagcatcct gcgcgcctgt
    18301 gttcactgcg gattttgtac cgcaacctgc ccaacctatc agcttctggg cgatgaactg
    18361 gacgggccgc gcgggcgcat ctatctgatt aaacaggtgc tggaaggcaa cgaagtcacg
    18421 cttaaaacac aggagcatct cgatcgctgc ctcacttgcc gcaattgtga aaccacctgt
    18481 ccttctggtg tgcgctatca caatttgctg gatatcgggc gtgatattgt cgagcagaaa
    18541 gtgaaacgcc cactgccgga gcgaatgctg cgcgaaggat tgcgccaggt agtgccgcgt
    18601 ccggcggtct tccgtgcgct gacgcaggta gggctggtgc tgcgaccgtt tttaccggaa
    18661 caggtcagag caaaactgcc tgctgaaacg gtgaaagcta aaccgcgtcc gccgctgcgc
    18721 cataagcgtc gggttttaat gctggaaggc tgcgcccagc ctacgctttc gcccaacacc
    18781 aacgcggcaa ctgcgcgagt gctggatcgt ctggggatca gcgtcatgtc agctaacgaa
    18841 gcaggctgtt gtggcgcggt ggactatcat cttaatgcgc aggagaaagg gctggaacgg
    18901 gcgcgcaata atattgatgc ctggtggccc gcgattgaag caggtgccga ggcaattttg
    18961 caaaccgcca gcggttgcgg cgcgtttgtc aaagagtacg ggcagatgct gaaaaacgat
    19021 gcgttatatg ccgataaagc gcgtcaagtc agtgaactgg cggtcgattt agtcgagctt
    19081 ctgcgcgagg aaccgctgga aaaactggca gttcgcggcg ataaaaaact ggccttccac
    19141 tgcccgtgta ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa agtgttgctt
    19201 cgtcttggat ttaccttaac ggacgttccc gacagccatc tgtgctgcgg ttcagcggga
    19261 acatatgcgt taacgcatcc cgatctggca cgccagctgc gggataacaa aatgaatgcg
    19321 ctggaaagcg gcaaaccgga aatgatcgtc accgccaaca ttggttgcca gacgcatctg
    19381 gcgagcgccg gtcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca agcccttgaa
    19441 aaggaataac aaaatgaaaa ctaaagtcat tcttagccag caaatggcga gtgcaattat
    19501 tgccgcaggt caggaagagg cgcagaaaaa taactggtcg gtttccattg ctgttgccga
    19561 tgacggcggt catctgctgg cgttaagtcg catggacgat tgcgcgccga ttgcggctta
    19621 catctcccag gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta agggctatga
    19681 agagatggtg aacaacggac gtaccgcgtt cgtgactgcg ccgttactga cgtcgctgga
    19741 aggcggcgta ccagttgttg tggatgggca aattattggt gccgtgggcg tttctggttt
    19801 aaccggagca caggatgcgc aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc
    19861 gaaaacgagg agataaacaa tgagtcaaac cataacccag ggccgtttac gcattgacgc
    19921 caattttaaa cgttttgtgg atgaagaagt tttaccggga acagggctgg acgctgcggc
    19981 gttctggcgc aattttgatg aaatcgttca cgatttggca ccagaaaatc gtcagttgct
    20041 ggcagaacgc gatcgcattc aggcggcgct tgatgagtgg catcgcagca atccggggcc
    20101 ggtaaaagat aaagcggcct ataaatcttt cctgcgtgaa ctgggctacc tggtgccgca
    20161 actggagcgc gtgacggtgg aaaccacggg cattgacagc gaaatcacca gtcaggcggg
    20221 gccacagctg gtggttccgg caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg
    20281 ctggggctca ctgtacgatg cgttatacgg cagcgacatc atcccgcagg aaggggcgat
    20341 ggtcagcggc tacgatccgc aacgcggtga gcaggttatc gcctgggttc ggcgtttcct
    20401 cgatgaatct ctaccgctgg aaaacggcag ctatcaggat gtggtggcgt ttaaggtggt
    20461 cgataaacaa ttacgcatcc agttgaaaaa tggtaaagaa accacgttac gtaccccggc
    20521 gcagtttgtc ggttaccgtg gcgatgccgc tgcgctgacc tgcattttgc tgaaaaataa
    20581 cggcctgcat attgaactgc aaattgatgc caacgggcgg attggcaaag acgatccggc
    20641 gcacatcaac gatgttatcg tcgaagcggc catcagtacc attctcgact gcgaagattc
    20701 ggtcgcggcg gttgatgcgg aagataaaat cctgctgtac cgcaatctgc tgggcctgat
    20761 gcaggggact ctgcaagaga aaatggagaa aaacggtcga caaatcgtgc gtaaactgaa
    20821 tgacgatcgt cattacaccg ccgccgatgg ctctgaaatt tctctgcacg gacgctcgct
    20881 gctgtttatc cgcaacgtgg gtcatttgat gaccattcct gtgatttggg acagcgaagg
    20941 caatgaaatc ccggaaggca ttcttgatgg cgtcatgact ggcgcgatcg ccctctatga
    21001 tttaaaagtg cagaaaaact cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca
    21061 cggtccgcag gaagtggcgt tcgccaacaa actgtttacc cgcattgaga caatgctcgg
    21121 tatggcaccg aataccctga aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa
    21181 cttgcgtagc tgtatcgctc aggcgcgcaa tcgcgtggcg ttcatcaata ccggtttcct
    21241 cgaccgtacc ggcgatgaaa tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa
    21301 ccagatgaaa tcgacgccgt ggatcaaagc ctacgagcgt aataacgtgc tttccggtct
    21361 gttctgtggg ctgcgcggta aagcgcaaat tggtaaaggc atgtgggcaa tgccggacct
    21421 gatggcagac atgtacagcc agaagggcga ccaactgcgt gccggggcaa acacagcctg
    21481 ggttccgtca ccaaccgctg ctacgctcca tgcgctgcac taccaccaaa ccaacgtaca
    21541 gagcgtgcaa gccaacattg cccagaccga gttcaatgct gaatttgaac cgctgctgga
    21601 cgatctgctg actattccgg tggctgaaaa cgctaactgg tcggtggaag agatccaaca
    21661 agagctggat aacaacgtgc aggggattct ggggtacgtg gtgcgctggg tggagcaggg
    21721 gattggttgt tcaaaagtgc cggatattca caatgtggcg ctgatggaag accgcgcaac
    21781 gctgcgtatc tccagccagc atatcgccaa ctggttacgt cacggtattc tgaccaaaga
    21841 acaggtgcag gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa acgctggcga
    21901 tccggcttat cgtccgatgg cggggaattt cgctaactcg tgtgctttta aagctgccag
    21961 cgatttaatc ttcctcggcg tgaaacagcc aaacggctat accgaaccgt tattacacgc
    22021 ctggcgttta cgcgaaaaag aaagtcatta atagcgtcaa aaaaagcccc ggcaagtttt
    22081 attgtcgggg ctatgatagt aactgcggta gtacatttat cccggaaata accggagcga
    22141 ttatcagggt ttaacaatat taaatacccg tagcgcatta ctagcgggcc taaaacaaca
    22201 atatggacat cttaacgata ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat
    22261 atacttgtta tgccagaggt agcaattgcg ttagcggcgt actgttgctg taaagtataa
    22321 cgcctatatt ttacagttca gtcctctttt gcttattaca accttaagta tatcaagcat
    22381 ataaagataa taagagactg aacaatatgg ttacctggac ccaaatgtat atgccgatgg
    22441 gaggactggg gctatccgct ctggtcgccc tgatcccgat aatattcttc ttcgttgcac
    22501 tcgcggtatt acgtctgaaa ggacatgtcg ctggagcaat aacccttatc ttatctatcc
    22561 tgattgcgat attcgccttt aaaatgccga ttgatatggc atttgctgct gcaggctatg
    22621 gctttattta tggattatgg ccaattgcgt ggattattgt cgcggcggtg ttcctctata
    22681 aattaaccgt tgccagcggt cagttcgata ttatccgcag ctcggttatc tccatcaccg
    22741 acgatcagcg tttgcaggtg ttactgattg gtttctcctt tggtgcgttg ctggaaggag
    22801 cggctggctt tggtgcgccg gtggcgatta ccggtgcgct gctggtgggc ctgggcttca
    22861 aaccgttata cgcggcgggg ctgtgtctga ttgccaacac tgcgccagtg gcgtttggtg
    22921 cgttgggcgt gccgattctg gtcgccggtc aggtaacggg aatcgatccg ttccacattg
    22981 gcgcaatggc gggacgtcag ttaccgttcc tgtcggttct tgtgccgttc tggctggtag
    23041 caatgatgga cggctggaaa ggggtgaaag agacgtggcc agcggcgctg gttgctgggg
    23101 gaagcttcgc tgtcactcag ttctttacct ctaactatat tggtccggaa ctgccggata
    23161 ttacttcggc gctggtgagt atcgtttcac tcgctttatt ccttaaagtc tggcggccga
    23221 aaaataccga aacggcaatc agcatggggc aatccgcagg tgcgatggtg gtaaataagc
    23281 catcttctgg cggtcccgtg ccttcagaat atagtctggg gcaaatcatt cgtgcgtggt
    23341 caccgttttt aatcttaacg gtgctggtca ccatctggac catgaagccg tttaaagcgt
    23401 tatttgctcc gggcggcgcg ttttattcac tggtgattaa tttccagatc cctcatttgc
    23461 atcaacaagt gttgaaagcg gcacccattg tcgcccaacc aacgccaatg gatgcggtgt
    23521 ttaaattcga ccctctctcg gctggcggca ccgctatttt tattgcggcg attatctcta
    23581 tcttcatcct cggcgtgggc atcaagaaag ggattggcgt ctttgccgaa acgctaatta
    23641 gtctgaagtg gccgatattg tcgattggca tggtgctggc gttcgccttc gtcaccaact
    23701 attccggcat gtccaccacg ttggcgctgg tactggcagg tacaggcgtg atgttcccgt
    23761 ttttctcacc gttcctcggc tggctggggg tattccttac cggctcggac acttcttcaa
    23821 acgccctgtt tggttcactg caatcgacca cggcgcagca aatcaacgtc tctgacactc
    23881 tgctggtggc agcaaatacc agcggcggcg taactggcaa gatgatctcc ccgcaatcta
    23941 tcgccgtggc ctgcgccgcg acgggcatgg tgggccgaga atctgaacta ttccgctaca
    24001 ccgtgaagca cagtctgatt tttgccagcg ttatcggcgt tatcacgttg ttgcaggcgt
    24061 atgtgtttac tggaatgttg gtctcgtaa
//
LOCUS       DAFAVF010000058        23795 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFAVF010000058 DAFAVF010000000
VERSION     DAFAVF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.058"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..3710
                     /gene="yfaL"
                     /locus_tag="LGK42_002296"
     CDS             <1..3710
                     /gene="yfaL"
                     /locus_tag="LGK42_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBK2658539.1"
                     /translation="YLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCHASRQSLSGI
                     TQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNN
                     GGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAMFRNNIAND
                     GKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIIN
                     NTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPST
                     AAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNN
                     DFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGST
                     QQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMA
                     LTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLE
                     VSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQI
                     ASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHGRDIEMRADG
                     EVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLK
                     GDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLRLTGQDTSVA
                     LNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNG
                     VAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPT
                     STGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSP
                     PDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYRNNLRAANQAFMMERRDHAGGNGQTL
                     NLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQGES
                     RSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDH
                     YHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQT
                     RLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNIS
                     QRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3838..4560)
                     /locus_tag="LGK42_002297"
     CDS             complement(3838..4560)
                     /locus_tag="LGK42_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBK2658540.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4707..7334
                     /gene="gyrA"
                     /locus_tag="LGK42_002298"
     CDS             4707..7334
                     /gene="gyrA"
                     /locus_tag="LGK42_002298"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBK2658541.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7483..9171
                     /locus_tag="LGK42_002299"
     CDS             7483..9171
                     /locus_tag="LGK42_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBK2658542.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQNKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9168..9791
                     /locus_tag="LGK42_002300"
     CDS             9168..9791
                     /locus_tag="LGK42_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBK2658543.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9815..14329
                     /locus_tag="LGK42_002301"
     CDS             9815..14329
                     /locus_tag="LGK42_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBK2658544.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSYAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14330..15979
                     /locus_tag="LGK42_002302"
     CDS             14330..15979
                     /locus_tag="LGK42_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBK2658545.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKKPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            15984..16760
                     /locus_tag="LGK42_002303"
     CDS             15984..16760
                     /locus_tag="LGK42_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBK2658546.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17035..19884
                     /gene="rcsC"
                     /locus_tag="LGK42_002304"
     CDS             17035..19884
                     /gene="rcsC"
                     /locus_tag="LGK42_002304"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBK2658547.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20084..20734)
                     /gene="rcsB"
                     /locus_tag="LGK42_002305"
     CDS             complement(20084..20734)
                     /gene="rcsB"
                     /locus_tag="LGK42_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBK2658548.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20751..23423)
                     /gene="rcsD"
                     /locus_tag="LGK42_002306"
     CDS             complement(20751..23423)
                     /gene="rcsD"
                     /locus_tag="LGK42_002306"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBK2658549.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQIPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5785 a         5812 c         6507 g         5691 t
ORIGIN      
        1 agtatttatc tctactcccg tcaatgattg catctctttt ctctgctaac ggtgtcgcgg
       61 cggccattga tttatgccag ggatatgata tcaaagcgag ttgtcacgcc agcaggcaaa
      121 gcctttcagg cattacgcag gactggagtg tcgccgatgg gcaatggctg gttttttcgg
      181 atatgaccaa taatgccagc ggaggagccg tatttttgca acaaggagcg gaattttcac
      241 tattaccaga aaatgaaact ggaatgactc tgtttgccaa taacaccgtt acaggagaat
      301 ataataacgg cggggccata tttgctaaag aaaactcaac gctgaatctt actgatgtta
      361 ttttttccgg taacgtcgca ggcggctatg gtggcgcaat ctattcttct ggtactaacg
      421 atactggtgc cgtcgattta cgtgtcacta acgccatgtt tcgcaataac atcgctaatg
      481 atggcaaagg tggcgcaatt tataccatta ataatgacgt ttatttaagt gatgttattt
      541 ttgataacaa ccaggcatat acatcaacaa gttacagtga tggcgatggc ggggcaatcg
      601 atgttaccga taataatagc gacagcaagc atccttcagg ttatacgata ataaataaca
      661 ccgcctttac aaataacact gccgaaggtt atggcggggc gatatatacc aatagcgcga
      721 cggctcccta tcttattgat atttctgttg atgacagcta cagccagaac ggcggcgtgt
      781 tagtggatga gaacaacagc gcagcaggct atggagatgg tccttccact gcggcgggtg
      841 gctttatgta tctcggctta agtgaagtta cctttgatat tgccgacgga aaaacgctgg
      901 ttattggcaa tacagagaat gacggagctg ttgactctat tgctggtacc gggttgatca
      961 ccaaaacagg ttccggcgat ctggtactta atgcagataa caatgacttt actggtgaga
     1021 tgcagattga aaacggtgaa gttaccctgg gccgcagcaa ctccctgatg aatgtcggcg
     1081 atacgcattg ccaggacgat ccgcaagact gctacggtct gacgataggg agtattgatc
     1141 agtatcagaa tcaggctgag ctaaacgttg gctcgaccca acaaactttt gtgcacgcat
     1201 tgacgggctt tcagaatggc actttaaata tcgatgctgg tggcaacgtt actgttaatc
     1261 agggcagttt tgctggcatc atcgaaggtg ctggtcagct caccattgcg caaaacggca
     1321 gctacgtgct ggcaggggcg cagtcgatgg cgctaaccgg cgatatagtc gttgatgatg
     1381 gtgcggtgct ttcgctggaa ggcgacgcgg cagatcttac cgctctccag gacgatccgc
     1441 agtcgatcgt gttaaacggc ggtgtgctcg atctctctga tttctccacc tggcagagcg
     1501 gcacatcata caacgatggc cttgaagtca gtggcagcag cggaacggtt atcggcagtc
     1561 aggatgtggt agatcttgca ggtggcgaca atttgcatat cggcggcgac gggaaagatg
     1621 gcgtctacgt ggtggtcgat gcgagcgacg ggcaggtaag tctggcaaac aataatagtt
     1681 atttgggcac aacacaaatc gcctccggta cgctgatggt gagcgacaac tcgcagcttg
     1741 gagataccca ctataaccgc caggttatct ttaccgataa gcaacaagaa agcgtgatgg
     1801 agattacctc cgacgttgac acgcgttcag atgcggcagg ccacggacgt gatattgaaa
     1861 tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga cacgcagtgg ggcgcactga
     1921 tggctgacag cagcgggcag catcaggatg agggtagcac attgactaaa acgggggctg
     1981 gtacactgga gctgaccgcc agcggtacaa cgcagtcggc ggtacgtgtc gaagaaggca
     2041 ccctgaaagg tgatgttgct gatatccttc cttatgcttc gtcactgtgg gttggtgatg
     2101 gggcaacgtt cgttactggc gcggatcagg atattcagtc aattgatgct atttccagcg
     2161 gcactatcga catcagcgat ggtacggttt tgcgcctgac cgggcaggat acttccgtcg
     2221 cccttaatgc ctcactattt aacggcgatg ggacgctggt gaatgccacc gatggtgtga
     2281 cgttgacagg tgagcttaat accaaccttg aaactgacag cctgacttat ctttccaacg
     2341 tgacggttaa tggcaatctg accaatacgt ccggtgcggt tagcctgcaa aatggcgtcg
     2401 ctggcgatac gctgacggta aacggtgatt ataccggcgg cggtacgcta ctgctcgata
     2461 gcgaattaaa cggcgatgac tcggtaagcg atcaattggt gatgaacggt aatactgctg
     2521 gcaacacaac tgtggtggtt aactccatta cagggattgg tgagccgaca tcgacaggca
     2581 ttaaagtggt tgatttcgca gctgatccca cgcagtttca aaacaatgcg cagttcagtc
     2641 tggcaggcag cggctacgtc aatatgggag cgtatgacta cacgctggtg gaagataaca
     2701 acgactggta tctgcgatcg caagaagtaa cgccgccatc gccacctgat ccagaccctg
     2761 acccaacacc cgatcctgac ccaacacccg atccaatccc cgcttaccag ccggtgctga
     2821 atgccaaagt tggcggttat cgcaataatc tgcgggcggc aaatcaggcg tttatgatgg
     2881 agcgacgcga tcatgcaggt ggcaatggtc agacgctgaa tttacgtgtt atcggcggac
     2941 gttatcatta cacggcggca gggcaactgg ctcagcatga agacacttct acggtgcagc
     3001 ttagcggtga cctgtttagc gggcgctggg gcgatgatgg cgagtggatg cttggggcgg
     3061 ttggtggcta cagtgataat cagggcgaga gccgctcgaa tatgaccgga actcgcgccg
     3121 ataaccagaa ccacggttat gccgttgggc tgacctcaag ctggtatcag cacggtaatc
     3181 agaagcaggg ggcctggctg gatagctggc tgcaatacgc gtggtttagc aatgatgttt
     3241 ccgaacaaga agatggcaca gatcattacc actcgtcggg gattatcgcc tcgctggagg
     3301 cggggtatca gtggttaccg gggcgtggtg tggtgattga accgcaggcg caggtgattt
     3361 atcagggcgt gcagcaggat gattttaccg ccgctaaccg tgcgcgcgtg tcacaatcgc
     3421 agggtgatga tattcagacg cggctgggtt tacacagcga atggcgtacc gctgttcatg
     3481 tcataccaac attagatctg aattattatc acgatcccca ttcgacggaa attgaagagg
     3541 atggcagcac tatcagtgac gatgcggtga agcaacgggg tgaaataaaa gtgggagtca
     3601 cgggcaatat cagtcagcga gtgtccctgc gcggcagcgt ggcgtggcag aaagggagtg
     3661 atgattttgc ccagacggca gggtttttgt cgatgacggt gaaatggtaa tcactgtcta
     3721 gtcggcacgg ttgccggatg cggcgtaaac gccttatccg gcctacgggg agcatttgta
     3781 ggcctgataa gacgcggcaa gcgtcgcatc aggcattggc tcggccaaac aacaacctca
     3841 cttattctgc gtgtgcagca tatagttcac atccacgccg gggccgagtt taaaagtatt
     3901 agtgagcggg ttgtaatgta gcccggttat atgtcgctct ttaaggcttg tctgatctac
     3961 ccagccaagc agttctgccg gtttaataaa cttcttcaca tcatgcgtgc ctttgggcac
     4021 catgcgcaaa atatattccg cgccaaccac cgccatcagc catgacttgc cgttgcggtt
     4081 aagcgtcgag aaaaagacat cgccgcctgg tttcaccagt tgcgcacagg ctctgaccac
     4141 tgactgcgga tcggggacgt gctccagcat ctccatgcag gtcaccacat catactgccc
     4201 ggcatgtttt gccgcgtgct cttccacggt ttcctgcaca taatccacct gaatgccgct
     4261 ttccagcgcg tgcagttttg ccacctgcaa tggctcaaag cccatatcca gaccggtcac
     4321 cgtcgcgcct tcgcgcgcca tactctcggc cagaatgccg ccgccacaac cgacatcgag
     4381 cacctttttg ccaaataaac cgccagcacg ctcggcaata tagcccagac gcagcgggtt
     4441 aatgcggtgc aacggtttga actcaccttc aagatcccac cagcgggagg cgacggcttc
     4501 aaatttagcg atctcttcgt ggtctacgtt atggttttcc ggcgattttt cggcattcat
     4561 tggcacttct actccgtaat tggcaagaca aacgagtata tcaggcattg gatgtgaata
     4621 aagcgtatag gtttacctca aactgcgcgg ctgtgttata atttgcgacc tttgaatccg
     4681 ggatacagta gagggatagc ggttagatga gcgaccttgc gagagaaatt acaccggtca
     4741 acattgagga agagctgaag agctcctatc tggattatgc gatgtcggtc attgttggcc
     4801 gtgcgctgcc agatgtccga gatggcctga agccggtaca ccgtcgcgta ctttacgcca
     4861 tgaacgtact aggcaatgac tggaacaaag cctataaaaa atctgcccgt gtcgttggtg
     4921 acgtaatcgg taaataccat ccccatggtg actcggcggt ttatgacacg atcgtccgta
     4981 tggcgcagcc attctcgctg cgttacatgc tggtagacgg tcagggtaac ttcggttcca
     5041 tcgacggcga ctctgcggcg gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc
     5101 atgaactgat ggccgatctc gaaaaagaga cggtcgattt cgttgataac tatgacggca
     5161 cggaaaaaat tcccgacgtc atgccaacca aaattcctaa cctgctggtg aacggttctt
     5221 ccggtatcgc cgtaggtatg gcaaccaaca tcccgccgca caacctgacg gaagtcatca
     5281 acggttgtct ggcgtatatc gatgatgaag acatcagcat tgaagggctg atggaacaca
     5341 tcccggggcc ggacttcccg acggcggcaa tcattaacgg tcgtcgcggt attgaagaag
     5401 cttaccgtac cggtcgcggc aaggtgtata tccgcgctcg cgcagaagtg gaagttgacg
     5461 ccaaaaccgg tcgtgaaacc attatcgtcc acgaaattcc gtatcaggta aacaaagcgc
     5521 gcctgatcga gaagattgcg gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc
     5581 tgcgtgacga gtctgacaaa gacggtatgc gcatcgtgat tgaagtgaaa cgcgatgcgg
     5641 tcggtgaagt tgtgctcaac aacctctact cccagaccca gttgcaggtt tctttcggta
     5701 tcaacatggt ggcattgcac catggtcagc cgaagatcat gaacttgaaa gacatcatcg
     5761 cggcgtttgt tcgtcaccgc cgtgaagtgg tgacccgtcg tactattttc gaactgcgta
     5821 aagctcgcga tcgtgctcat atccttgaag cattagccgt ggcgctggcg aacatcgacc
     5881 cgatcatcga actgatccgt catgcgccga cgcctgcaga agcgaaaact gcgctggttg
     5941 ctaatccgtg gcagctgggc aacgttgccg cgatgctcga acgtgctggc gacgatgctg
     6001 cgcgtccgga atggctggag ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg
     6061 aacagcaagc tcaggcgatt ctggatctgc gtttgcagaa actgaccggc cttgagcacg
     6121 aaaaactgct cgacgaatac aaagagctgc tggatcagat cgcggaactg ttgcgtattc
     6181 ttggtagcgc cgatcgtttg atggaagtga tccgtgaaga gctggagctg gttcgtgaac
     6241 agttcggtga caaacgtcgt actgaaatca ccgccaacag cgcagacatt aacctggaag
     6301 atctgatcac tcaggaagat gtggtagtga cgctctctca ccagggctac gttaagtatc
     6361 agccgctttc tgaatacgaa gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta
     6421 ttaaagaaga agactttatc gaccgactgc tggtggcgaa cactcacgac catattctgt
     6481 gcttctccag ccgtggtcgc gtctattcga tgaaagttta tcagttgccg gaagccactc
     6541 gtggcgcgcg cggtcgtccg atcgtcaacc tgctgccgct ggagcaggac gaacgtatca
     6601 ctgcgatcct gccagtgacc gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta
     6661 acggtaccgt gaagaaaact gtcctcaccg agttcaaccg tctgcgtacc gccggtaaag
     6721 tggcgatcaa actggttgac ggcgatgagc tgatcggcgt tgacctgacc agcggcgaag
     6781 acgaagtaat gctgttctcc gctgaaggta aagtggtgcg ctttaaagag tcttctgtcc
     6841 gtgcgatggg ctgcaacacc accggtgttc gcggtattcg cttaggtgaa ggcgataaag
     6901 tcgtctctct gatcgtgcct cgtggcgatg gcgcaatcct caccgcaacg caaaacggtt
     6961 acggtaaacg taccgcagtg gcggaatacc caaccaagtc gcgtgcgacg aaaggggtta
     7021 tctccattaa ggttactgaa cgtaatggtt tagtggttgg cgcggtacag gtagatgact
     7081 gcgaccagat catgatgatc accgatgccg gtacgctggt acgtactcgc gtttcggaaa
     7141 tcagcatcgt aggccgtaac acccagggcg tgatcctcat ccgtactgcg gaagatgaaa
     7201 acgtagtggg tctgcaacgt gttgctgaac cggttgacga ggaagatctg gataccatcg
     7261 atggcagtgc cgcggaaggg gacgatgaaa tcgctccgga agtggacgtt gacgacgagc
     7321 cagaagaaga ataattttac ttcttcatgc caaaagggag ctatctccct tgtttgaatt
     7381 gaaaagtcca ggctgcaaag tctgggcttt tgtcgtatta gggcacggta aagtttggct
     7441 gtgcccgtaa aaaatggctg gctatacaca aggaatgtgg caatgagtgg tgaaaaaaag
     7501 gcgaaaggct ggcggttcta tggtcttgta ggttttggcg caatagcact gctttccgct
     7561 ggcgtctggg cgttgcaata tgctggcagt gggccagaaa aaacgttgtc gccgctggtg
     7621 gtgcacaaca atctgcaaat cgatctcaat gagccggacc tctttctcga cagcgactct
     7681 ctgagccagc ttcccaaaga tctcctcacc attccgtttc tccacgatgt tctgagcgaa
     7741 gatttcgttt tctattatca gaatcatgcc gatcgtctgg gcattgaagg cagcattcgt
     7801 cgtattgtct atgaacacga tctcacgctg aaagataagc tcttttcgtc actcttagat
     7861 cagcccgcgc aggcagcgct gtggcacgat aaacaaggcc atctttcaca ttatatggtg
     7921 ctgatccagc gcagtggttt aagcaaactg ctggagccat tgttgtttgc cgctaccagc
     7981 gacagccagt taagcaaaac ggaaatcagt agcatcaaga taaatagtga aactgttcct
     8041 gtttatcagt tgcgctataa cggcaataac gccctgatgt tcgcgactta tcagaacaag
     8101 atgctggtgt tttccagcac ggatatgttg tttaaagatg atcagcagga taccgaagcc
     8161 acggcgatcg caggtgattt gttgagcggc aaaaaacgct ggcaagcaag ctttggcctg
     8221 gaagagcgtg ctgctgaaaa aacgccagta cgccagcgca tcgtggtcag cgccaggttg
     8281 ctgggttttg gctaccagcg gttaatgcct tcttttgctg gcgtacgctt cgaaatgggt
     8341 aacgacggct ggcacagttt tgtggcgtta aatgatgaat ccgccagcgt agataccagt
     8401 ttcgatttta cgccggtctg gaacagtatg cctgccgggg ccagcttctg tgtggtggtg
     8461 ccgtattcac acggtattgc cgaagagatg ctttcgcaca tcagtcagga aaacgacaag
     8521 ttgaatgggg cgttagacgg tgccgcgggg ctgtgctggt atgcagactc aaaattgcaa
     8581 accccgctgt ttgtcggtca gtttgatggc actgccgaac aggcgcaatt gccagggaaa
     8641 ctgtttacgc aaaatattgg tgcgcacgaa agcaaagcgc cagaaggtgt tttgccgata
     8701 agccagactc agcagggcga agcgcaaatc tggcgtcgcg aagtgagttc ccgatacggt
     8761 cagtatccga aagcgcaggc ggcgcaaccc gatcaattaa tgtcggatta ttttttccgc
     8821 gtgtcgctgg cgatgcaaaa caaaacgctg cttttctccc tcgatgacac gctggttaat
     8881 aacgccctgc aaacactgaa taaaaaccgc ccggcaatgg tggatgtaat acccactgat
     8941 ggcatcgttc cgctctatat caatccacaa ggcgtggcga aactgctgcg taacgaaacg
     9001 ttgaccagtc tgccgaagaa tctcgaaccg gttttttata acgccgcaca aactttatta
     9061 atgccgaagc tggacgcttt atctcaacaa ccgcgttatg tcatgaagct ggcccagatg
     9121 gaacccggtg ccgcctggca gtggctaccc ataacctggc agccgctatg aggcacgggc
     9181 tgctggcgct gatttgctgg ctgtgttgtg ttgttgccca tagcgaaatg ctgaatgtcg
     9241 aacaatccgg gctgtttcgc gcctggtttg tacgcattgc acaagaacag ctccgccagg
     9301 ggccaagtcc acgctggtat cagcaggatt gtgcgggcct ggtgcgattt gcggcgaacg
     9361 agacgctgaa agttcacgat agtaaatggc taaaaagtaa cggtttctct agccagtatt
     9421 tgccgccaga gatgacgcta acacctggac agcgtcaact ggcgcaaaac tggaatcagg
     9481 ggaacgggaa aaccggcccc tacgtgaccg cgattaattt gattcagtac aacagccagt
     9541 ttattggcca ggacataaac caggcgctgc ctggcgatat gatttttttc gatcagggtg
     9601 atgcccagca cttaatggtc tggatggggc gttacgtcat ctaccacacc ggaagcgcca
     9661 cgaaaactga caacggaatg cgcgcagtca gtctgcaaca acttatgaca tggaaggaca
     9721 cccgatggat acccaacgat tccaatccca atttcattgg catttatcgt ttaaattttc
     9781 tggcgcgata gcagcgtgct tgtccctgtc tcttgtggga acagggcttg ctaatgctga
     9841 tgattcgctt ccttccagta actatgcgcc gcccgccggg ggaacattct ttttgcttgc
     9901 tgacagcagt tttagcagca gtgaagaggc gaaagtgcga ctggaagcgc ccgggcgtga
     9961 ttatcggcgc tatcagatgg aagagtacgg cggcgtggac gttcgcctgt atcgtattcc
    10021 tgacccgatg gcatttttgc gccagcagaa aaacctgcat cgcattgtgg tgcaaccgca
    10081 atatctgggc gacgggctga acaatacgct gacctggctg tgggataact ggtacggcaa
    10141 atctcgccgc gtaatgcagc gtactttctc ttctcagtca cggcagaatg tgactcaggc
    10201 attacccgaa ttacatctcg gcaatgccat tattaaacct tcccgttatg tacagaacaa
    10261 ccagttttcc ccgctgaaaa aatatccctt ggtggaacag ttccgttatc cactatggca
    10321 ggctaaaccg gtcgagccgc agcaaggggt aaaactggaa ggcgcatcca gcaatttcat
    10381 ctcgccgcag ccgggtaaca tttatattcc tctcggccaa caagagccgg gactgtacct
    10441 cgtcgaggcg atggttggtg ggtatcgggc gacgacggtg gtgtttgttt ccgataccgt
    10501 ggcgcttagc aaagtgtcag gcaacgagct tctggtgtgg accgcgggta aaaaacaggg
    10561 tgaagcgaag cccggctcag agatcttgtg gactgacggt cttggcgtga tgacccgcgg
    10621 tgtgaccgat gacagcggta ccttgcagtt acaacatata tcgccagaac gttcatacat
    10681 tctgggtaag gatgctgaag gcggtgtttt tgtctccgag aacttcttct acgaaagcga
    10741 aatctacaac acccgcttgt atatttttac cgatcgcccg ctatatcgcg caggcgatcg
    10801 tgtcgatgtt aaagtgatgg gccgcgagtt ccacgatccg ttgcattcat cccccatcgt
    10861 cagcgccccg gcgaagcttt cggtgctgga cgctaacggc agtctgttgc aaaccgtcga
    10921 tgtcacgctg gatgcgcgca atggcgggca gggaagtttc cgcctgccag aaaatgccgt
    10981 tgccggaggt tatgagttac gtcttgctta ccgcaatcag gtctatagca gcagttttcg
    11041 cgtggcaaac tacatcaagc cacatttcga gattggttta gctctcgaca aaaaagagtt
    11101 caaaactggc gaagcggtca gcggcaaact gcaactgctc tacccggatg gcgagccggt
    11161 aaaaaatgcc cgcgtgcagt taagtttgcg cgctcagcaa ttatcaatgg tcggtaacga
    11221 tttgcgttat gccggacgtt tccccgtgtc gctggaaggc agcgaaacgg tgtccgacgc
    11281 cagcggtcat gtggcgttaa atctccccgc cgccgataaa ccgagccgtt atttgttaac
    11341 cgtctccgcc agtgacggcg cggcgtatcg tgtcaccacc accaaagaga tcctcattga
    11401 acgcggtctg gcgcattact cattaagtac tgccgcacaa tacagtaata gcggcgagtc
    11461 ggttgtgttc cgttatgccg cgctggaatc ttcaaaacag gttcctgtta cgtatgaatg
    11521 gttgcgtctc gaagaccgca cgagccatag cggagagcta ccgtcaggcg gcaaatcctt
    11581 taccgtcaat ttcgctaaac ctggcaacta caatctgaca ttacgcgata aagacggctt
    11641 aattctcgct gggttaagtt atgccgtcag cggtaagggc agcacggcgc atacgggtac
    11701 ggtagatatc gtggcggata aaacgctgta ccagccaggc gaaaccgcga agatgctgat
    11761 tacctttccg gagccaattg atgaagcatt attgacgctg gaacgcgatc gcgtggaaca
    11821 gcagtcgctg ctttcgcatc cggcaaactg gctaacgcta caacgtttaa acgataccca
    11881 gtatgaagcc cgggttccag tgagcaattc ctttgcgcct aacatcactt tttcggtgct
    11941 gtatacccgt aatggtcagt acagttttca gaacgccggg atcaaagttg ccgttcctca
    12001 gcttgatatc cgggtgaaaa cggacaaaac ccattaccag cctggtgaac tggtcaatgt
    12061 cgaattaacc tcgtcgctga aaggtaaacc tgtttctgcg cagctaacgg taggcgtggt
    12121 cgatgaaatg atctacgcgc tgcaaccaga aatcgcgccg aatatcggca aatttttcta
    12181 tccgctgggg cgtaacaatg tgcgtaccag ctccagtctg tcgtttatca gctacgacca
    12241 ggcgctctcc agcgagccgg ttgcgcctgg cgcaactaac cgcagcgagc ggcgagtaaa
    12301 aatgcttgaa cgtccacggc gtgaagaggt ggataccgcg gcatggatgc cgtcactcac
    12361 aaccgataaa caaggcaaag cgtatttcac gttcctgatg cctgattcgt taacccgctg
    12421 gcgtatcacc gcgcgtggga tgaacggcga cgggctggtc gggcaggggc gtgcttatct
    12481 gcgttcggaa aaaaatctct acatgaagtg gagtatgcca acggtgtatc gcgtgggcga
    12541 caaaccggcg gcaggactgt ttatcttcag tcagcaggat aacgaaccgg tggcgctggt
    12601 gactaaattt gcaggcgctg agatgcgcca gacgctgacg ctgcacaaag gggcgaatta
    12661 tatttcgctg gcgcagaaca ttcagcaatc tggcttgtta agcgcagaac tgcaacaaaa
    12721 tgggcaagtg caggacagca ttagcacaaa actgtctttt gtggataaca gctggcccgt
    12781 tgaacagcag aaaaatgtca tgctcggcgg tggcgagaac gcgctgatgt tgcccgagca
    12841 ggctagcaat atccggctac aaagtagcga aacgccgcag gagattttcc gcaacaatct
    12901 tgatgcgtta gtcgatggac catggggtgg cgtgatcaac accggtagcc gtctgatccc
    12961 gctcagtctc gcctggcgtt cgcttgccga tcatcaaagt gccgccgcta acgacattcg
    13021 tcagatgatt caggataacc gtctgcggct gatgcaactg gcggggcccg gagcgcgctt
    13081 tacctggtgg ggtgaagatg gcaatggtga cgccttcctt acggcatggg catggtacgc
    13141 cgactggcag gccagccagg cgctcggcgt aacgcaacaa ccggaatact ggcagcatat
    13201 gctcgacagt tatgccgagc aggcggataa catgccgtta ttgcatcggg cgctggtgct
    13261 ggcgtgggca caggagatga atctgccgtg caaaacgttg ttgaaagggt tggatgaagc
    13321 tatcgcccgg cgcggaacta aagatgaaga tttctctgag gaagacatcc gcgatatcaa
    13381 tgacagcctg atcctcgata caccggaatc tccactggca gatgcggtgg caaacgtctt
    13441 aaccatgacg ttgctgaaaa aagcgcagtt gaagtccacg gtgatgccac aggttcagca
    13501 atatgcgtgg gataaagcgg caaacagcaa tcagccgctg gcgcacacgg ttgtgctgct
    13561 caatagcggt ggtgacgcta cccagacggc cgctatttta agtggtttga ccgctgagca
    13621 atccactatt gagcgcgcgc tggccatgaa ctggctggcg aaatatatgg cgacaatgcc
    13681 tccagttgtt ttgcctgcgc ctgcgggcgc atgggctaaa cataagttaa ctggaggggg
    13741 cgaagactgg cgttgggttg ggcagggtgt gccggacatt ctctcttttg gtgacgaatt
    13801 atcgccgcaa aatgtgcagg tccgctggcg tgaaccggca aaaacggctc aacaaagtaa
    13861 cattccggtg accgttgaac gccagttgta tcggcttatc cccggtgaag aagagatgag
    13921 ctttactctg cagccggtga ccagcaatga gattgacagc gatgcgctgt atctcgatga
    13981 aattacgctt accagcgagc aggatgcagt tctgcgctat ggtcaggtgg aagtaccgct
    14041 cccgccggga gccgacgttg agcgcacaac atggggcatt tcagtcaata aacccaacgc
    14101 cgcgaaacag caggggcaat tgctggaaaa agcgcgtaat gaaatgggcg aactggccta
    14161 tatggtgccg gtgaaagaac tgacgggaac ggtcactttc cgccatttgc tgcgcttctc
    14221 gcaaaaaggg caattcgttc tgcctcctgc tcgttatgtg cgttcctatg cacctgcgca
    14281 gcaaagtgtt gcggcaggga gtgaatggac cgggatgcag gtgaaataag tgaactggcg
    14341 cagaatcgtc tggctattgg cgttggttac tctgccaacg cttgcggagg agacgccgtt
    14401 acaactggtg ctgcgcggtg cgcagcacga tcaactgtat cagctgtcgt catcaggcgt
    14461 gaccaaagtc tcagcattac ctgactcgct caccacgccg ttagggagtt tgtggaaact
    14521 gtatgtttac gcctggctgg aggataccca tcaaccggag cagccctacc agtgtcgcgg
    14581 aaactccccg gaagaggttt attgttgtca ggctggggag agcataacgc gcgataccgc
    14641 gctggtacgt tcctgtgggc tctattttgc gccgcagcgg ttgcatattg gcgcagatgt
    14701 gtgggggcaa tactggcaac agcgtcaggc acctgcgtgg ttagcgtctc ttacaacgct
    14761 gaagccagaa acctcggtaa cggttaaatc gctgctcgat tcattagcga cgttacctgc
    14821 gcaaaacaaa gctcaggaag tcttactgga tgtggtgctg gacgaggcga aaatcggtgt
    14881 cgcatccatg ctcggtagtc gggtacgggt gaaaacctgg agctggtttg ccgatgataa
    14941 acaggaaatc cgccagggtg gttttgctgg ctggttaacc gatggtacac cgctgtgggt
    15001 tactggcagt ggcaccagca aaacggtact aacccgttat gcgacggtgc ttaatcgggt
    15061 tttgccagtg ccgacgcagg ttgccagtgg gcaatgtgta gaggtggagc tgtttgcccg
    15121 ctatccgctg aagaaaatca cagcggaaaa gagtacgacg gcagtaaaac ccggcgtact
    15181 taatgggcgc tatcgtgtga cgtttactaa tggtaaccac attacgtttg tcagccacgg
    15241 tgagacgacc ttactgagcg aaaagggcaa actgaaattg cagtcacatc tcgatcgtga
    15301 agagtacgtt gccagagttc tggatcgcga ggcgaaaagt acgccccctg aggcagcgaa
    15361 agccatgacc gtggcgattc gcactttttt acagcaaaac gccaatcggg aaggcgattg
    15421 cctgaccatc cctgacagca gcgccacgca gcgtgtttcg gcttcgccag ctacgacggg
    15481 ggcgcgaacg atggccgcct ggacgcagga tctcatctat gcgggcgatc cggttcatta
    15541 tcatggcagt cgcgctaccg aagggacgct ttcctggcgg caagccacgg cccaggcggg
    15601 gcagggagag cgttacgacc agatactcgc gtttgcttat cccgacaaca gccttagccg
    15661 ctggggagcg ccacgctcaa cctgccagtt attacccaaa gcgaaagcct ggctggcgaa
    15721 aaaaaagccg cagtggcggc gtatattaca agctgagacg gggtacaacg aaccagacgt
    15781 gtttgcggtt tgccgtttag tctcaggttt cccctatacc gatcggcagc agaaacggct
    15841 gtttattcgt aacttcttta cgcttcagga tcggctggat ttaacccatg agtatctgca
    15901 cctggccttt gatggttatc ccaccgggct tgatgagaac tatatcgaaa cgctgacccg
    15961 acaattatta atggactaat gctatgcgaa agatttttct tccgttgtta ctggtggcac
    16021 tttcgcctgt cgttcacagt gaaggtgtgc aggaagtcga gattgacgca ccgctctctg
    16081 gctggcatcc ggtagagggc gaagatgcca gtttttcaca atcgatcaat tacccggcgt
    16141 cgtccgtcaa catggcagat gatcaaaata tctcagcgca gatccgcggc aaaataaaga
    16201 attatgctgc ggcgggtaaa gttcagcagg gccggctggt ggtcaacggt gccagtatgc
    16261 cgcagagaat tgaatccgat ggttcatttg cacgccctta tattttcact gaaggcagca
    16321 acagcgtgca ggtcatcagc ccggatgggc aaagccgaca aaaaatgcag ttttactcaa
    16381 cgccgggtac aggaacgatt cgtgcacgtt tacggctggt tctctcgtgg gatacggaca
    16441 ataccgatct cgatctccat gttgtcacgc ccgatggcga acacgcctgg tacggtaaca
    16501 ccgtgctgaa aaacagtggt gcactggata tggatgtcac gacggggtac ggacccgaga
    16561 ttttcgccat gccagcacca gttcacgggc gctatcaggt gtatatcaac tactatggtg
    16621 ggcgcagcga aacggaattg acgaccgccc aactgacgct gatcaccgat gaaggatcgg
    16681 tcaatgagaa acaggaaaca tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg
    16741 tgaaaagttt tgactggtga tccatccctt cacacaacct ggctctctct tttttcagat
    16801 aagactgctg ggcgatctgc cgacgccagg atataaccat taacaagcgt cggcagattg
    16861 gtcatttctt atttttcgta ctgagaactg tccatctact ccggctttcc tggttaaggc
    16921 aatggtttct aagcgatacg cattattgca atggtgacag aacattgcgg taacacgctt
    16981 ttaccgctac cttaaccaca ctccatcggt cacctgaggc ggagcttcgc ccctttgaaa
    17041 taccttgctt cttttcgtac aaccctgaaa gcctcgcgct acatgttcag agcattggcg
    17101 ttagtgctct ggctgttgat tgctttttca tccgtttttt acatcgttaa tgcgttacat
    17161 cagcgagaat cggaaattcg tcaggaattt aatctgagtt ccgatcaggc tcagcgcttt
    17221 attcaacgca cctctgatgt gatgaaagag ctgaagtaca tcgccgaaaa tcgcttatcg
    17281 gcagaaaacg gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt gcctgcgttt
    17341 gaaccgctgt ttgccgactc cgattgttcc gcaatgagta acacctggcg aggttctctg
    17401 gagtcattgg cgtggtttat gcgctactgg cgcgataatt tttctgcggc ttacgatctc
    17461 aaccgggtat ttttaatcgg cagtgataac ctctgcatgg ccaatttcgg tctgcgtgat
    17521 atgccagtgg aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa taaatatcga
    17581 aatgcaccac aagatgatag cggcagtaac ctctactgga tcagcgaagg tccgcgccct
    17641 ggcgtcgggt atttttacgc gttgacgcca gtttatctgg cgaaccggtt gcaggcgctt
    17701 ttgggtgtcg agcagaccat ccggatggag aactttttct taccgggtac gttgccgatg
    17761 ggggttacca ttcttgatga aaatggtcat accctgattt cgcttaccgg accagaaagt
    17821 aaaattaagg gcgatcctcg ctggatgcag gaacgctcct ggtttggcta tacggaaggg
    17881 ttccgggagc tggtgctgaa gaaaaatctg ccaccctcat cgctaagcat cgtgtattcg
    17941 gtgccggttg ataaggtgct ggaacgcatt cgcatgttga tccttaacgc aattttgctg
    18001 aatgtgcttg ccggagctgc attgtttact ctcgcgcgga tgtacgagcg acgtattttc
    18061 attccggcgg aaagcgacgc cctgcgactg gaagaacatg agcagttcaa tcgcaagatt
    18121 gtcgcctccg cgccagtggg tatctgcatt ttgcgtaccg ctgatggcgt caatatttta
    18181 agtaacgaac tggcgcatac ctatctcaat atgcttacgc atgaggaccg ccaacgactg
    18241 acgcaaatta tctgtgggca gcaggtcaat tttgttgatg tcctgaccag caacaatacc
    18301 aatctgcaaa tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc catttgtgtg
    18361 ctggtggatg tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat ggcacaagca
    18421 gcggaacagg cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca tgagctgcga
    18481 acgccgctgt atggcattat cggtaacctg gatctgttgc aaaccaaaga gttaccgaaa
    18541 ggcgtcgatc ggctggtgac ggcaatgaac aactcttcca gcctgttgtt gaaaattatc
    18601 agcgatattc tcgatttctc gaagattgaa tcggaacagt tgaagatcga accgcgtgag
    18661 ttttcaccgc gtgaagtgat gaaccacatc accgccaact atttaccgct ggtggtacgc
    18721 aagcagttag gcttgtactg ctttattgaa ccggatgtgc cagtggcctt aaatggcgac
    18781 ccgatgcgtt tacagcaggt catctccaac ctgttgagta acgccataaa attcaccgat
    18841 accggctgta tagttttgca tgtccgcgct gatggcgatt atctctctat ccgtgttcgc
    18901 gataccggcg tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc cttcttccag
    18961 gtcggaacgg gcgtacagcg aaacttccag gggaccggtc tgggtctggc gatttgtgaa
    19021 aaactgatca gcatgatgga cggcgatatc tcggtagatt cagaaccggg aatgggcagc
    19081 cagtttaccg tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa aggcgtggaa
    19141 gggttgagtg gtaaacgctg ctggctggcg gtccgcaatg cgtcgctctg tcagttcctg
    19201 gaaaccagtt tgcagcgcag cggcatcgtc gttacaacat acgaagggca ggaaccgact
    19261 cccgaagatg tgttaattac tgacgaggta gtgagtaaaa aatggcaggg tagagcggta
    19321 gtgaccttct gtcgtcgcca tattggtatt ccgctggaga aagcgccagg ggagtgggta
    19381 cacagtgtgg ctgccccgca tgagctaccg gcattgttgg cgcgtattta tttgatcgag
    19441 atggagagcg acgatcctgc taacgctctg ccgtcgacgg acaaagcggt cagcgataat
    19501 gacgatatga tgattctggt cgtggatgat catccgatta accgacgtct gctggcagat
    19561 cagttgggat cgttgggcta tcaatgtaaa accgcgaatg atggtgtcga tgcgcttaat
    19621 gtacttagca agaatcatat tgatatcgtg cttagcgacg tcaacatgcc aaatatggat
    19681 ggttaccgct tgacgcaacg cattcgtcag ttgggactga cgttgccggt aatcggagta
    19741 actgctaatg cgttggctga agagaagcag cggtgtctgg agtccggtat ggacagctgc
    19801 ctgtcgaagc cggtaacgct ggatgtgata aaacagacgc tgacggtata tgccgagagg
    19861 gtcaggaaat cgcgggaatc gtaggacgga taaggtgttt atgccgcatc tggcattcag
    19921 tgcaaatgcc agatgcgacg ctgacgcgtc ttatctggcc tactttaatg taatctgttg
    19981 aaataatggg aatcgtaggc cggataaggc gtttacgctg catccggcaa tcggtgcaaa
    20041 tgccagataa gacactaacg cgtcttatct ggcctacaga tgattagtct ttatctgccg
    20101 gacttaaggt cacagaagag agataattca gcagggcgat atcgttctcg acacccagct
    20161 tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc agctttttag
    20221 cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg
    20281 agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga gaaacgcttt
    20341 ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg
    20401 caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg
    20461 ggttgttgtt catggtcaga acaatgatcg acaggcttgg gaaatggcgc ttgatgtact
    20521 tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca
    20581 catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga
    20641 caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat
    20701 ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg ctacagcaag
    20761 ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca
    20821 cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca
    20881 aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct
    20941 tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg
    21001 ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc
    21061 gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag
    21121 ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc
    21181 aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta
    21241 attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca
    21301 atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc
    21361 tcttcttcct cttcaacttc cgggtcagct gcgagcattt tgatatgcac agagtagcgt
    21421 gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg tgccagttga
    21481 tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg
    21541 ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc cgtgtccaga
    21601 atgcggaacg tcaggcggtc ttcggaggac tcatcctgat caacctcaag ggtgattttt
    21661 cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaaggca
    21721 tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga
    21781 cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg
    21841 gagaacagca ccgtctcact tttccagcta tcatccgcaa gcatgttcgc taactgtatt
    21901 tcatcaacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg
    21961 ttgagtttag ccgcgctctc cgccagggac tgtgcgggtt ctttcagcgc atcgccaatg
    22021 tttttcataa aggtcatccg cccctgctgg tttttctcat acagacgctg cgcctgcttg
    22081 agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc
    22141 ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga
    22201 ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc
    22261 aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc
    22321 aggccgagcg gcagcagtga gactatctct tcattaattg cccgtaaaat gcgcaattcg
    22381 ttattgaccg ccgtgctggg tagactttct gtactgcggc tggagaaatg gcggaatgtg
    22441 gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg
    22501 ttttgcaacg tatccagcaa taaggtgcca taaggaatct gccagaccag gcgcatatcg
    22561 gtagagttga gtgccgaggc gatttcaatc ttcgtactgt taaagtggat actgacgcta
    22621 tccgttcctt ctttctcatt atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg
    22681 aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg
    22741 accgttgcca gatgtcctgg ctggttaaag gtagtacgca atgtaaagta atgaccgttc
    22801 tgccaggcca ggcggcgcat gttagaaaag ctttcgcgtt catcgagggc gttggcctgt
    22861 tgcaacatct ctgcacgacg tgaatcaaca atgtcgctga cggtcgattc tttaaatccg
    22921 gaggtgagat ctttaagggg tagggttgag atcagcacca gactgttatc ctgaccattc
    22981 aggtaataca tcgaccacgg tacattttct gcgccccaca atgtatccag ataagtggac
    23041 atccgctgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt
    23101 ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct
    23161 tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa
    23221 cgccagttat cgacgcgctt ttggatagca tgggtaatgt cgacaatctg gtaactttta
    23281 tctttcagcc aggcgttaac ggcgctttgt accattacac ccatcgtcac cagtaacaca
    23341 atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg
    23401 gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata
    23461 gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat
    23521 tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct
    23581 gtacaaataa cctacaaaaa gcccaacaaa aataattgca aataaacaaa gattgctgga
    23641 aattatgcgg atgttaatta tttgtgaaat agttaacaag cgttatagtt tttctgtggt
    23701 agcacagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccgaatgc gaggcatccg
    23761 gttgaaatag gggtaaacag acattcagaa atgaa
//
LOCUS       DAFAVF010000059        23600 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFAVF010000059 DAFAVF010000000
VERSION     DAFAVF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.059"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            158..1873
                     /locus_tag="LGK42_002307"
     CDS             158..1873
                     /locus_tag="LGK42_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBK2658550.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1870..3363
                     /locus_tag="LGK42_002308"
     CDS             1870..3363
                     /locus_tag="LGK42_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBK2658551.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(3410..3859)
                     /locus_tag="LGK42_002309"
     CDS             complement(3410..3859)
                     /locus_tag="LGK42_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658552.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            3969..4316
                     /locus_tag="LGK42_002310"
     CDS             3969..4316
                     /locus_tag="LGK42_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBK2658553.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            4306..4668
                     /locus_tag="LGK42_002311"
     CDS             4306..4668
                     /locus_tag="LGK42_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBK2658554.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            4665..5162
                     /locus_tag="LGK42_002312"
     CDS             4665..5162
                     /locus_tag="LGK42_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK2658555.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QLRRDCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5170..6354)
                     /gene="emrD"
                     /locus_tag="LGK42_002313"
     CDS             complement(5170..6354)
                     /gene="emrD"
                     /locus_tag="LGK42_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBK2658556.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(6773..6862)
                     /gene="tisB"
                     /locus_tag="LGK42_002314"
     CDS             complement(6773..6862)
                     /gene="tisB"
                     /locus_tag="LGK42_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBK2658557.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            7427..7525
                     /gene="ivbL"
                     /locus_tag="LGK42_002315"
     CDS             7427..7525
                     /gene="ivbL"
                     /locus_tag="LGK42_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBK2658558.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            7631..9319
                     /gene="ilvB"
                     /locus_tag="LGK42_002316"
     CDS             7631..9319
                     /gene="ilvB"
                     /locus_tag="LGK42_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBK2658559.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            9323..9613
                     /gene="ilvN"
                     /locus_tag="LGK42_002317"
     CDS             9323..9613
                     /gene="ilvN"
                     /locus_tag="LGK42_002317"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBK2658560.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            9688..10278
                     /gene="uhpA"
                     /locus_tag="LGK42_002318"
     CDS             9688..10278
                     /gene="uhpA"
                     /locus_tag="LGK42_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBK2658561.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            10278..11780
                     /gene="uhpB"
                     /locus_tag="LGK42_002319"
     CDS             10278..11780
                     /gene="uhpB"
                     /locus_tag="LGK42_002319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBK2658562.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            11790..13109
                     /gene="uhpC"
                     /locus_tag="LGK42_002320"
     CDS             11790..13109
                     /gene="uhpC"
                     /locus_tag="LGK42_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBK2658563.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            13247..14638
                     /gene="uhpT"
                     /locus_tag="LGK42_002321"
     CDS             13247..14638
                     /gene="uhpT"
                     /locus_tag="LGK42_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBK2658564.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(14683..16449)
                     /gene="adeD"
                     /locus_tag="LGK42_002322"
     CDS             complement(14683..16449)
                     /gene="adeD"
                     /locus_tag="LGK42_002322"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK2658565.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            16624..17958
                     /gene="adeQ"
                     /locus_tag="LGK42_002323"
                     /gene_synonym="yicO"
     CDS             16624..17958
                     /gene="adeQ"
                     /locus_tag="LGK42_002323"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBK2658566.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            18011..18463
                     /locus_tag="LGK42_002324"
     CDS             18011..18463
                     /locus_tag="LGK42_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBK2658567.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            18674..19864
                     /gene="nepI"
                     /locus_tag="LGK42_002325"
     CDS             18674..19864
                     /gene="nepI"
                     /locus_tag="LGK42_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBK2658568.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(19905..20198)
                     /locus_tag="LGK42_002326"
     CDS             complement(19905..20198)
                     /locus_tag="LGK42_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658569.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            20420..21238
                     /gene="nlpA"
                     /locus_tag="LGK42_002327"
     CDS             20420..21238
                     /gene="nlpA"
                     /locus_tag="LGK42_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBK2658570.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(21242..22165)
                     /locus_tag="LGK42_002328"
     CDS             complement(21242..22165)
                     /locus_tag="LGK42_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2658571.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(22276..23460)
                     /locus_tag="LGK42_002329"
     CDS             complement(22276..23460)
                     /locus_tag="LGK42_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HBK2658572.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         5464 a         5906 c         6363 g         5867 t
ORIGIN      
        1 cctccggcga attatgctca caacctgaac ttaacagaaa acaatccgtt cagggatacg
       61 acgtttgtat tttaagaatc tgactgcctg acccgacgca ttttacctct ccctatattc
      121 atgcgtccgg gacataaaca taaataaggg ctatgagatg aattcgttac aaatcttgag
      181 ttttgtcggt tttacgctgc tggtggcggt gatcacctgg tggaaggtcc gcaaaacaga
      241 taccggatcg caacaaggct attttcttgc cggacgttca ctaaaagcgc cggttattgc
      301 cgcttcgtta atgctaacca acctttccac ggaacaactg gtcggccttt ccgggcaggc
      361 ctacaaaagc ggcatgtcgg tgatgggctg ggaagtgact tcagcggtga cgctgatctt
      421 cctcgcgcta atctttttac cgcgctatct gaagcgcggc attgccacca tccccgattt
      481 tctggaggaa cgttatgata aaacgacgcg tattatcatc gacttctgct tcctcattgc
      541 caccggcgtc tgctttctgc cgattgttct ctactccggc gcgttggcgc tcaacagcct
      601 gtttcacgtc ggggaatcgc tacagatttc tcacggtgcg gctatctggc tactggtaat
      661 tttgcttggt ctggcgggaa ttttgtatgc ggtgatcggc ggactgcgcg caatggcagt
      721 ggcggactcc atcaacggta ttgggctggt tatcggcggg ttgatggtgc cggtatttgg
      781 cctgatcgcg atgggcaagg gcagctttat gcagggcatt gagcaactca ccaccgttca
      841 cgccgagaaa ttaaactcaa tcggtggccc gaccgatccc ttgccgattg gcgcggcatt
      901 taccggtttg attctggtga acacctttta ctggtgtaca aatcagggca tcgtgcaacg
      961 cacgctggcg tcaaaaagcc tggcggaagg gcaaaagggg gcgctgttaa cggcggtgct
     1021 gaaaatgctc gacccgctgg tactggtgct gccagggttg attgcgtttc atctgtatca
     1081 ggatttaccg aaagccgaca tggcctaccc gacgctggtc aataacgttc tgccagtgcc
     1141 aatggtgggt ttcttcggcg cggtgttatt tggtgcggtg atcagtacct tcaacggctt
     1201 tctgaatagc gccagtacgt tattcagtat gggtatttac cgtcgcatca ttaaccagaa
     1261 tgccgagccg cagcagctgg tcaccgtcgg gcgcaaattt ggtttcttta tcgctatcgt
     1321 ttcggtgctg gtcgcgccgt ggatcgccaa cgcgccgcag gggctgtata gctggatgaa
     1381 acagctcaac ggcatttaca acgtgccgct ggttaccatc atcattatgg gctttttctt
     1441 cccgcgcatc ccggcgctgg cggcaaaagt ggcgatgggg attggcataa tcagctacat
     1501 caccatcaac tatctggtga agttcgactt ccatttcctc tatgtgctgg cctgtacgtt
     1561 ctgcatcaac gtggtcgtga tgctggtgat cggttttatc aaaccgcgcg ccacgccgtt
     1621 caccttcaaa gatgcgtttg cggtggacat gaaaccgtgg agaaacgtca agatcgcgtc
     1681 aattggcatc ctgttcgcga tgattggcgt ctatgccggg ctggctgaat tcggcggcta
     1741 cggtacgcgc tggttagcga tgatcagtta tttcattgcc gccgtagtga ttgtctacct
     1801 gatttttgac agctggcggc atcgtcacga cccagccgta acctttactc ccgacgcgaa
     1861 ggatagccta tgaaacgccc caattttctg ttcatcatga ccgataccca ggccaccaat
     1921 atggtcggtt gctatagcgg taaaccgctg aatacgcaaa atattgatag tctggcggcg
     1981 gaaggtattc gctttaattc cgcctacacc tgttcaccgg tttgtacgcc tgcacgcgcc
     2041 ggactattta ccggtatcta cgctaaccag tccggcccgt ggaccaacaa cgtcgcgcca
     2101 ggcaaaaaca tctccactat ggggcgctac tttaaggatg ccggctatca cacctgttac
     2161 atcggcaaat ggcatctcga cggtcatgac tatttcggca ctggcgagtg tccgccggag
     2221 tgggacgctg attactggtt cgatggggcg aactacctta gcgaactgac ggaaaaagag
     2281 atcagcctgt ggcgcaatgg cctaaacagc gttgaggatt tacaggcgaa ccatatcgac
     2341 gaaaccttca cctgggcgca ccgcatcagc aatcgggcgg tagattttct gcaacagccc
     2401 gcgcgcgccg aggaaccctt cctgatggtg gtttcgtatg atgagccgca tcacccgttc
     2461 acctgtccgg tggagtattt agagaaatac gctgattttt actacgatct gggcgagaaa
     2521 gctcaggatg acctggcgaa caaaccggaa catcaccgct tatgggcgca ggcgatgcca
     2581 tcgccagtcg gtgatgacgg gctttatcac catccgctct attttgcctg taatgacttt
     2641 gttgatgacc aaatcggacg ggtcatcaat gccttaacgc cagagcaacg tgaaaatacg
     2701 tgggtcattt atacttccga tcacggcgaa atgatgggcg cacataagct gatcagtaaa
     2761 ggagcggcga tgtatgacga catcacccgt attccgctga tcatccgttc gccgcaaggg
     2821 gagcggcgac aggtcgatac gccagtcagt catatcgatt tactgccgac aatgatggcg
     2881 ctggcagata ttgaaaaacc agagattctg ccgggggaaa atatccttgc cgtgaaagag
     2941 ccacgcggtg taatggtgga atttaaccgc tacgagattg agcatgacag ctttggcggt
     3001 tttattccgg tgcgttgctg ggtgacggat gactttaaac tggtactcaa cctcttcacc
     3061 agtgatgaac tttacgatcg ccgtaatgac ccaaatgaaa tgcataacct gatcgatgat
     3121 atccgttttg cagacgttcg cagcaaaatg catgacgcct tattggatta catggacaaa
     3181 attcgcgatc cgttccgcag ttaccaatgg agcctgcgtc cgtggcgtaa agatgcactg
     3241 ccgcgctgga tgggggcatt tcgtccacgc ccacaagatg gctattcgcc ggtggtacgt
     3301 gactatgaca ccggcctacc gacgcaagga gtgaaagtgg aagagaaaaa acagaagttc
     3361 tgacgataat gcccgctgat gaacacccgg cgggcattat tcaggctgct taaaccagtg
     3421 aaccgccggt caggaaggaa aaaatcagga atatcagcaa aatgctgcac acgatgccga
     3481 taccaaaaag caaggtatgg aaagccagcg tcccgccgcc aaaaaccagc gcgccgcgaa
     3541 tgccccagcg attttcctta cgcataccgc caataaatgc gcaaagtagc gcagcaatgg
     3601 ccagtgcgat accgctttga tcgaggattt tatcgacatc tacgtcctct tttttctcca
     3661 cggtggtaat tttttcgcct tttattccgg cgagaagacc tcttttaatt tcagccgttt
     3721 tgtccgccac gatgctttca aacgttggcg gtggtgcgga aaaagggccg aatgaaaagt
     3781 ggataatccc catcattaaa gcgatggcac cgaacagcat gcccaatgaa ctgattttat
     3841 tttgagctat gatacccatc tgacctcctt gtagcggatg gcgaaagcat atcaggaaac
     3901 gggcggacga aattcttaaa aaaaaaccgc taaaggtaga gcgttagtga aattgcaaag
     3961 gagcaacaat gaaaatttcc cgccttggag aagcgcccga ttaccgcttc tcgctggcta
     4021 atgagcgtac ttttctggcg tggatccgta ccgcactagg atttctggcg gcgggggttg
     4081 ggcttgatca acttgcgcca gatttcgcca cgcccgtcat tcgcgaactg ctggcgttgt
     4141 tgttgtgtct gttttccggc gggttagcga tgtatggcta cttgcgctgg ttacgcaatg
     4201 aaaaagcgat gcgtctgaaa gaggacctgc cttacaccaa cagcttatta atcatcagtt
     4261 taatattgat ggtcgtggcg gtgatcgtta tgggactggt gttgtatgcc ggatagccgc
     4321 aaagccagac gcattgccga cccaggactg cagccggagc gtacatcact ggcgtggttt
     4381 cgtaccatgc tgggctacgg cgcgttgatg gcgttggcta tcaaacacaa ctggcaccag
     4441 gcgggcatgt tattctggat ttcgattggc atcctcgcca tcgtggcgct gatcctctgg
     4501 cactacactc gtaatcgcaa tttaatggat gtcacgaata gcgatttttc ccaatttcac
     4561 gtagtccgtg acaaattttt gatctccctc gcggtgttat ctctcgcaat actgtttgct
     4621 gtaacgcata tacatcaact tatcgtattt attgagagag tcgcatgaca ggaagtcagg
     4681 tcattgatgc tgaagaggac aggcataagc tggttgtaga gtataaggat gctctgcaac
     4741 ctgccgattt ttatcacaat ttcaaacaac ggggcatccg ctccgtacag ctcattcccc
     4801 atcttgaatt tgatgaccgg ggcgatctga cggccgcttc ggtgacagca gagctgtggg
     4861 ggaaattttt aattgccctg ttcgagtgtt gggtgcgggc agatatcagt cgtatatcga
     4921 ttgagctttt tgacgctacc ctacaaaaat ggtgtggcag cgaaaacccg caactgcgcc
     4981 gtgactgcca ggcgtgcgac tggcatcggc tgtgtccgca cgctcgggag gagacgccag
     5041 acagcgtgct atgtgcgggc tatcaggcgt tttattctta ctccgcaccg catatgcggg
     5101 taatgcgtga tctgataaag cagcatcgtt ctcccatgga gctgatgacg atgctgcggt
     5161 gacgtgcgct taaacgggct gcccctgatg cgacatccgc gtcgccagcg gcagccagca
     5221 cagcacgatc aacaatccca ttaaggtcat caataacccc aggctgccct gaccggtttg
     5281 cggcaacatc gcagagagcg acgccagcac gccggaacca atgttttgca gaccgccgac
     5341 cagcgcgcca gccgtgcccg ccaggaaggg gaacggctcc atcgcgccgc tggtcgccag
     5401 cggaaacagc atcccggcac cgaaaaagaa cagcgcggcg ggaacgagca gcgtccagac
     5461 attcatcacg ccaaaccagt ctgggatcca catcagcaag ccagccagca ggcagcagat
     5521 aaccgactgc cacattaacg tggagaagcg tttattggga cgtccggcaa accatgcgcc
     5581 aaaaaacgct gccggaatcg gcagaataaa caaaatactg accgtcatac tgctcagccc
     5641 taacaccgcg cccatcagca cgccggagca ggcttcaaag gcggcaatcc cggccagacc
     5701 gccaatcagc atcagcaaat aacagttaaa accgctgtta ccgaaaagcg ttttataact
     5761 ggtaagcagg cgcgtgcgcg gtgcatcgac cggacgcgtt tccggcatcc agcgggccat
     5821 actgaaggtc acaccagcac aaagaaccaa caaaaagaga taacaggcgc gccagttcca
     5881 catcgtatcc agcagaccgc cgattagcgg tgcgagcaac ggactgacga gaatccccat
     5941 gtttaacagg ctgttagcat ggcgcaactg tgtccgttca tataaatctc gcggcaaagt
     6001 acgcgccatt acgccgccaa cgccggtgcc catcccctgc atcgcgctgg cggcaatcaa
     6061 caccgtcaaa ctggaggtcg tgaccgcgac cagcgttgcc agcataaaaa tggacattcc
     6121 gacgaggatc accggtcggc ggcccacgcg gtcggaaatc gggccataaa acagctgtga
     6181 gacaccgtaa gtcagcagat aagcgcccat tacgctctgc accgcccctt cacggacgtt
     6241 gagatcgcgc gccatatcgg caatagctgg aatataaatg gtttgcgcca tctgaccgac
     6301 ggccacgagt aataccaaca tcaataacaa attgacgttt ctttgccttt tcattatcac
     6361 ggatgctttt ataaaaataa ttagaataag tcactacgca ttcccataaa tgcgggagag
     6421 gaatctatca cattgatgaa tgttagccag atatacgcca ggaatgggga attgtttagc
     6481 ggcagaatat gtaaacaaaa gcggcaataa atgttgccgg gagggattac gtgcgaactg
     6541 taaccctctc cccaaagggg cgagggggaa accaccgttc cctttcagaa aatgagctaa
     6601 acattgcacg ggacggtccc ctcgcccctt tggggagagg gctagggtga ggggcacagg
     6661 cagcgcaggt gcaaacattc tgctggtaga agaagccccc aactccaacc cgcacgctaa
     6721 atataaaagg ggagcggtta cccgctcccc tttggtgcga cttgaatctg aattacttca
     6781 ggtatttcag aacagcatca agcagttgca gtgctgcaac aatgagtttg aggataagaa
     6841 tggcgatatc caccaggctc atacgcgtct cctgtggttc aggagcactg ccagctgacg
     6901 tacctttccg ctgcctgttg ccagctgttg tgcacgcggt atcttaagga gaaacgttgt
     6961 gcttgttgac ataacacagt gtgctcacgc ggggttaagg cgctgacggc accacccgtt
     7021 tcagccagga cttagtgcgc cgggtaacga tgcggccccc gcataacaca ttgcgtacag
     7081 tgatattata gaagtttact gtataaataa acagtaatat ttggacaaaa cgcaaactgt
     7141 gatcgatact caactatgcc ctaaatacgt caaaattcgt gccgaaattg cgcgttctgc
     7201 gcggaacacg tatactttca gtgttgacat aatacagtgt gctttgcggt taccagccgc
     7261 aggcggctga tgaaacctcg ctccggcggg gttttttgtt atctgcaatt cagtacaaaa
     7321 cgtgatcagc ccctcaattt tcccttgctg aaaaattttc cattgtctcc cctgtaaagc
     7381 tgtgcttgta taaatattgt taaacacaaa accaacaagg tccccaatga ctacttccat
     7441 gttcaacgca aaactactac caactgcgct atccgccgca gtggtcgtcg tgcgtgtggt
     7501 ggtggtcgtc ggcaatgcgc cgtagggact ggaacaacac acgattccaa aaccccgccg
     7561 gcgcaaaccg ggcggggttt ttcgtttaag cacctcccgg aaagtcggcc cagaagaaaa
     7621 ggactggagc atggcaagtt cgggcacaac atcgacgcgt aagcgcttta ccggcgcaga
     7681 atttatcgtt catttcctgg aacagcaggg cattaagatt gtgacgggca ttccgggcgg
     7741 ttctatcctg cctgtttacg atgccttaag ccaaagcacg caaatccgcc atattctggc
     7801 ccgtcatgaa cagggcgcgg gatttatcgc tcaggggatg gcgcgcaccg acggtaaacc
     7861 ggcggtctgt atggcctgta gcggaccggg tgcgactaac ctggtgaccg ccattgccga
     7921 tgcgcggctg gactccatcc cgctgatttg catcaccggt caggttcccg cctcgatgat
     7981 cggcaccgac gccttccagg aagtggacac ctacggcatc tctatcccca tcaccaaaca
     8041 caactatctg gtcagacata tcgaagaact cccgcaggtc atgagcgatg ccttccgcat
     8101 tgcgcaatca ggccgcccag gcccggtgtg gatagatatt cctaaggatg tgcaaacggc
     8161 agtttttgag attgaaacac agcccgctat ggcagaaaaa gccgccgccc ccgcctttag
     8221 cgaagaaagc attcgtgacg cagcggcgat gattaacgct gccaaacgcc cggtgcttta
     8281 tctgggcggc ggtgtgatca atgcgcccgc acgggtgcgt gaactggcgg agaaagcgca
     8341 actgcctacc accatgactt taatggcgct gggcatgttg ccaaaagcgc atccgttgtc
     8401 gctgggtatg ctggggatgc acggcgtgcg cagcaccaac tatattttgc aggaggcgga
     8461 tttgttgata gtgctcggtg cgcgttttga tgaccgggcg attggcaaaa ccgagcagtt
     8521 ctgtccgaat gccaaaatta ttcatgtcga tatcgatcgc gcagagctgg gtaaaatcaa
     8581 gcagccgcat gtggcgattc aggcggatgt tgatgacgtg ctggcgcaat tgatcccgca
     8641 ggtggaagcg caaccgcgtg cagagtggca ccagttggta gcggatttgc agcgcgaatt
     8701 cccgtgtcca atcccgaaag cgtgcgatcc attaagccat tacggcctga tcaacgccgt
     8761 tgccgcctgt gtcgatgaca atgcgattat caccaccgat gtgggtcagc atcagatgtg
     8821 gaccgcgcaa gcttatccgc tcaatcgccc acgccagtgg ctgacctccg gtgggctggg
     8881 cacgatgggt tttggcctgc ctgcggcgat tggcgcggcg ctggcgaacc cggatcgcaa
     8941 agtgttgtgt ttctccggcg acggcagcct gatgatgaat attcaggaga tggcgaccgc
     9001 cagtgaaaat cagctggatg tcaaaatcat tctgatgaac aacgaagcgc tggggctggt
     9061 gcatcagcaa cagagtctgt tctacgagca aggcgttttt gccgccacct atccgggaaa
     9121 aatcaacttt atgcagattg ccgccggatt cggcctcgaa acctgtgatt tgaataacga
     9181 agccgatccg caggctgcat tgcaggaaat catcaatcgc cctggcccgg cgctgatcca
     9241 tgtgcgcatt gatgccgaag aaaaagttta cccgatggtg ccgccaggtg cggcgaatac
     9301 tgaaatggtg ggggaataag ccatgcaaaa cacaactcat gacaacgtga ttctggagct
     9361 caccgttcgc aaccatccgg gcgtaatgac ccacgtttgt ggcctttttg cccgccgcgc
     9421 ttttaacgtt gaaggcattc tttgtctgcc gattcaggac agcgacaaaa gccatatctg
     9481 gctactggtc aatgacgacc agcgtctgga gcagatgata agccaaatcg ataagctgga
     9541 agatgtggtg aaagtgcagc gtaatcagtc cgatccgact atgtttaaca agattgcggt
     9601 gttttttcag taaccgctca aggcttgaac aacatcgcgc ttatcgttaa ggtaagcgcg
     9661 ttttttttta cccgccagga caagaccatg atcaccgttg cccttataga cgatcacctc
     9721 atcgtccgct ccggctttgc gcagctgctg gggctggaac ctgatttgca ggtagttgcc
     9781 gagtttggtt cggggcgcga ggcgctggcg gggctgccgg ggcgcggtgt gcaggtgtgt
     9841 atttgcgata tctccatgcc cgatatctcc ggtctggaac tgctaagcca gctgccgaaa
     9901 ggtatggcga caataatgct ctctgttcat gacagtccgg cgctggttga gcaggcgctt
     9961 aacgcggggg cacgcggctt tctctccaaa cgctgtagcc ctgacgaact gattgcggcg
    10021 gtgcatacgg ttgccacggg cggctgttat ctgacgccgg atattgccat taaactggca
    10081 tccggtcgtc aggacccgct aaccaaacgc gaacggcagg tggcggaaaa actggcgcaa
    10141 ggaatggcgg tgaaagagat tgctgccgaa ctgggcttgt cgccgaaaac ggtgcacgtc
    10201 catcgcgcca acctgatgga aaaactgggt gtcagtaacg acgtagagct ggcgcgccgc
    10261 atgtttgatg gctggtgatg aagacgttgt tctcccgctt aattaccgtt attgcctgct
    10321 tttttatctt ctctgccgca tggttttgcc tgtggagtat cagcctgcac ctggttgagc
    10381 gccctgatat ggcggtgctg ttatttccgt ttggtctgcg tctggggcta atgctgcaat
    10441 gcccgcgcgg atactggccg gtgctgctgg gcgcggagtg gctgctgatt tactggctaa
    10501 cgcaggcggt cggtttaacc cattttccgt tattgatgat cggtagttta ctgacgttac
    10561 tgcccgtggc gctgatctcg cgctatcgcc atcagcgtga ctggcgcacc ttgctgttac
    10621 agggtgcggc gttaacggcg gcggcgttgt tgcagtcgct gccctggctt tggcacggca
    10681 aagagtcgtg gaatgcgctg ttgctgactt taactggcgg cctgacgctg gccccgatat
    10741 gtctggtgtt ctggcactat ctcgccaata acacctggct gccgctcggc ccgtcactgg
    10801 tttctcagcc gatcaactgg cgcgggcgac atctggtctg gtacttgctg ctgtttgtta
    10861 tcagtctctg gctccagttg ggattgccgg acgaactgtc gcgctttacg ccattttgtc
    10921 tggcgctgcc gattatcgcg ctggcctggc actatggttg gcaaggggcg ctgattgcga
    10981 cgttgatgaa cgccatcgcg ctgatcgcca gtcaaacctg gcgcgatcat ccggtggatt
    11041 tattgctctc gctgctggtg caaagtctga cagggttgtt gcttggcgct ggcatccagc
    11101 ggttgcgtga acttaaccag tcgctgcaaa aggaactggc gcgcaatcag catctggctg
    11161 aacggttgct ggaaaccgaa gagagcgtgc gccgtgatgt ggcgcgtgag ctgcatgatg
    11221 atatcggtca gaccatcaca gccattcgta ctcaggcggg cattgttcag cggctggcgg
    11281 cagataacgc cagcgtgaag cagagcgggc agctcatcga acaactatcg ctgggcgttt
    11341 acgacgcggt gcgccgtttg ttgggtcggt tacgtccgcg ccagttggat gatctcaccc
    11401 tggagcaggc catccgctca ctgatgcggg aaatggagct ggaagggcgc ggtattgtca
    11461 gccatctcga atggcgaatc gatgaatcag cgttaagcga aaaccagcgc gtgacgctgt
    11521 ttcgtgtctg ccaggaaggg ctgaacaaca ttgtgaaaca tgctgatgcc agcgcggtca
    11581 ccctgcaagg ctggcagcag gatgaacggt tgatgctggt tattgaagac gatggcagcg
    11641 gtttgccgcc gggttccggg caacaaggtt ttggcctcac cggaatgcgc gagcgcgtaa
    11701 cggcgctggg tggcacatta cacatttcct gtctgcacgg cacgcgtgtc agcgtttctc
    11761 tacctcaacg ctatgtctaa ggtttgatga tgttgccgtt tctgaaagcg cctgccgatg
    11821 cgccattaat gactgataaa tatgaaattg atgcccgcta tcgctactgg cgtcggcata
    11881 ttctgctgac catctggctg ggttacgcgc tgttttactt cacgcggaaa agttttaacg
    11941 ccgcggtacc agaaatcctt gctaacggcg tgctcagccg tagcgatatc ggcctgttag
    12001 cgaccctgtt ttacattact tacggcgtgt cgaagtttgt ctccggcatt gtcagcgatc
    12061 gctcaaatgc ccgttatttt atggggatag ggcttatcgc cacgggcatc atgaacattc
    12121 tgtttggctt ctcgacgtcg ctatgggcgt ttgccgtgct ctgggtgctg aacgcctttt
    12181 tccagggctg gggttcaccg gtgtgtgcgc gtctgttaac ggcctggtat tcacgtaccg
    12241 agcgcggcgg ttggtgggca ttatggaaca cggcgcataa cgtcggcggc gcactcattc
    12301 ccattgtgat ggcagcgagc gcgctgcatt acggctggcg tgccgggatg atgattgctg
    12361 gttgtatggc gatagtcgtg gggatttttc tctgctggcg gctacgcgat cgcccgcagg
    12421 cgttaggttt accggcggtc ggtgaatggc ggcacgacgc gctggaaatt gctcaacaac
    12481 aagaaggggc agggttgacg cgtaaagaga tcctgaccaa atatgtgttg ctgaatccgt
    12541 atatctggct gctttcgttt tgctatgtgc tggtctatgt ggtccgggcg gcgatcaacg
    12601 actggggcaa tttgtatatg tccgagacac tgggcgtcga tctggtcacg gcgaatacgg
    12661 cagtgacgat gtttgaactg ggcggattta tcggtgcgct ggtagccggt tggggctcgg
    12721 acaaattgtt taacggcaac cgtgggccga tgaatttgat tttcgccgcc ggaattttgc
    12781 tttcagtcgg ctcgctgtgg ctgatgccat ttgccagcta cgtgatgcag gcaacctgct
    12841 tcttcaccat tggttttttt gtctttggcc cgcagatgtt aatcggcatg gcggcggcag
    12901 agtgttccca caaagaggcg gcaggggcgg cgacggggtt tgtcggcttg tttgcttatc
    12961 tgggggcgtc gcttgctggt tggccgctgg cgaaagtact cgatacctgg cactggagcg
    13021 gattttttgt ggttatctct atcgccgccg ggatttccgc actgctgtta ctgccctttt
    13081 tgaacgccca ggcaccgcgc gaagcgtgat gcatctcacc ttttcacttc atatccggca
    13141 aaactaagaa attttccagg ttttgcctgg acgctatctc aggcctgatt tgctgctgat
    13201 ttttacaatg catgcctcac gcaggtattc atttcaggag taacccatgc tggctttctt
    13261 aaaccaggtt cgcaagccga ccctggacct tccgctcgaa gtgcggcgca aaatgtggtt
    13321 caaaccgttc atgcaatcct acctggtggt ctttatcggc tacctgacga tgtacctgat
    13381 tcgcaagaac tttaacatcg cgcagaacga catgatctcg acctacgggt tgagcatgac
    13441 gcagctgggg atgatcggcc tgggtttctc catcacttat ggcgtgggta aaacgctggt
    13501 ttcctactac gccgacggca aaaacaccaa acaattcctg ccgttcatgc tgatcctgtc
    13561 tgccatttgt atgctgggct tcagtgccag tatgggcagc ggctcggtta gcctgttcct
    13621 gatgattgcc ttctacgcct taagcggctt tttccagagt accggcggtt cgtgcagtta
    13681 ctccaccatc accaaatgga caccgcgtcg taagcgcggg accttcctcg gtttctggaa
    13741 catttcccat aaccttggcg gcgcgggtgc ggctggtgtg gcgctgttcg gcgcaaatta
    13801 cctgttcgac ggccacgtga tcggcatgtt tatcttcccg tcgattatcg cgttgattgt
    13861 cggttttatc ggcctgcgtt acggcagcga ctccccggaa tcttatggcc tcggcaaagc
    13921 ggaagagctg ttcggcgagg agatcagcga agaggacaaa gagacagaat ctaccgatat
    13981 gaccaagtgg cagatctttg ttgagtatgt gctgaaaaac aaagtgatct ggctgctgtg
    14041 cttcgccaac attttcctct atgtggtgcg tattggtatc gaccagtggt caaccgtgta
    14101 cgcgttccag gaactgaaac tctctaaagc ggtggcgatt cagggcttta cgctgtttga
    14161 agctggtgcg ctggtcggta cgctgctgtg gggctggctc tctgacctgg cgaacggtcg
    14221 ccgtggcctg gtggcctgca tcgcgctggc gctgattatc gccacgctcg gtgtgtatca
    14281 acatgccagc aacgaatata tctatctggc ttctctcttt gcgttgggtt tcctggtctt
    14341 tggcccgcaa ttgttgattg gtgtggctgc tgttggcttt gtacctaaaa aagcgattgg
    14401 cgctgccgat ggtattaaag gcacctttgc ttacctgatt ggtgacagct ttgccaagtt
    14461 aggtctggga atgattgccg atgggacgcc ggtattcggc cttaccggct gggcaggcac
    14521 cttcgccgca cttgatatcg ccgcgattgg ttgtatctgc ctgatggcga tagtggcggt
    14581 gatggaagaa cgcaaaatcc gccgcgagaa aaaaattcag cagttgacag tggcataaac
    14641 gtaactggtg acttttgccc ggcatgacgc cgggcttttt tattattccg tgacttccag
    14701 cgtagtgaag gcaaacttct cgccatcaaa tagcccctga ctggttagtt ttagcgcggg
    14761 gatcactggc agagaaagaa acgccatctg aataaacggc tcatcgggta acggaccgca
    14821 ttcacgggcg gcggctttca aggcgtcaat ttgttccgcc agcaactgcg ccgtgtcggt
    14881 gctcatcagg ccggcaatgg gcaacggcag atgactttgg acctggccgt tacgtaccac
    14941 gcacagcccg ccgccatcct gaatcacctg attgaccgcc agcgccatct cttcggcact
    15001 gcgaccgatc accacaatat tatggctgtc atggctgacc gtcgcagcca gcgcaccttc
    15061 attcagtcca aagccgccaa gcaaaccaca agccggagcc agccgttgcc cgtaacgctc
    15121 aagtacggca ataaagctca catcatcgcg atcaaaacca ttttcgctgt agacgctgga
    15181 gtgggagtgc gtaatcaatt cgttatggat gacgtcaatg acccgatagc gttttccggg
    15241 cgtaaattgc agggcaaagt cgctggcgga aactggctgg cgggcaatgg tgttgccata
    15301 tggcggagcg gattgtgcca gtctcgccga ctcttccgcc tgtaaggttt gcgcatcaat
    15361 cggctcgcct ttcaccagta cctgctgcac cgtgacctta cgcgcatcgc tcaacaggac
    15421 gatatcggcc tgcttgccgg gtgccagtaa gccgaggtga ttcagaccaa agtggcgcgc
    15481 cgtcgaccag ctggcgacgc gatatgccac atgcagcggc acattgtgtt gttcgatcag
    15541 gcggcgaatt aaggcatcga tgtgtccttc atgggcgatc tcccacgggt tacggtcatc
    15601 ggtacagagc atgcattgcg ggctgttaaa ttcgttgatc aacggtgcca gcgcgttgag
    15661 attgcgggca gcggaccctt cgcggatcat caacgacatg ccgagttgta atttccggcg
    15721 tccttcttcc agctgataac tttcgtggca gttttcaata cccgcagtaa tataggcgtt
    15781 aagttcttta ccacccaaac ccgggcagtg accgtccagc gtcaggtggc gaaatgcatc
    15841 cagtttatcg agcagcgcat tctgcccgct aattacgcca gggtagtcca tcatttctgc
    15901 aaggccggta acctgcggat ggtcccgcca ggcgagcatc tgttcaaggg taaaactggc
    15961 accgttaaca tcgcagcctt ccagggcggg tacgcaagag ctgacctgta agtactggtt
    16021 ttgccttgcc tgttcggcac agcgggcaaa ccaggcgaat ccggcttctc ccatcacgtt
    16081 gacgatttca tgagggtcgc aaataacggt cgtcaggccg cgcggcaggg tagcggtttc
    16141 aaaagtgacc ggcgtcatca tgctggattc aatatgcagg tgagcatcaa taaaccctgg
    16201 caccgccgtt gcgccgcgag catcaatccg ctgcaaagcc ggagcatcag cgtattctgc
    16261 gccaacaccg gcaatgtaac gtcctttaat cacaattggg ccggaaattt ctccgccatt
    16321 gatcaggtcg agaatagaga cattatcaat aatataatcg gcaacagcgt cgccacggga
    16381 aacggctaac aattcctggt attcagcccg gctaatgtga tgaaatttat ggttaataga
    16441 attattcata tttttctcat tttgatttta tcaatttgat tattatttat cataaagaaa
    16501 cgtcgcgaga ttattaaaga atatccatta atgtgcaatt gaaatgtgat aattatcact
    16561 gaaccgttgt tgtcatttgt tttacaaaag caagggatta aatctcctcc aatggacaaa
    16621 aaaatgaata atgacaatac cgattacgtg agtaatgaat cagggacgct ttcgcgatta
    16681 tttaaactac ctcagcatgg gaccaccgtc cgcacagaat tgattgcggg gatgaccact
    16741 tttttaacca tggtgtacat cgtttttgtg aacccgcaaa tcctcggcgc ggcacaaatg
    16801 gacccgaaag tggtgtttgt taccacctgt ttgattgccg gtatcggcag tattgcgatg
    16861 gggatatttg ctaacttacc cgtggcgctg gctccggcaa tggggctgaa cgccttcttt
    16921 gccttcgtgg tcgtgggggc gatgggcatc tcctggcaga ccgggatggg cgcaatattc
    16981 tggggcgcag ttggactatt tttgctcacg ctgtttcgta tccggtactg gatgatctcc
    17041 aacattccct taagtttacg tattggtatc accagcggaa ttggattatt tatcgcctta
    17101 atgggattaa aaaatactgg cgttattgtc gccaataaag acacgctggt gatgattggc
    17161 gatttaagtt ctcacggcgt gttgttaggt attttagggt tttttattat aaccgtgttg
    17221 tcatcacgtc attttcatgc cgcggtgctg gtttctattg tggtgacgtc ttgctgtgga
    17281 ttatttttcg gtgatgttca ttttagcggc gtctattcca ttccgcctga tattagcggc
    17341 gtcattggtg aagtagattt gagcggcgcg ttaacacttg aactcgccgg tatcattttc
    17401 tcctttatgc taatcaacct atttgattca tcaggaacat taattggtgt aactgataaa
    17461 gcgggcttaa tagatagtaa cggtaaattc cccaatatga ataaggcgct gtatgttgat
    17521 agcgtcagtt cggtggcggg ggcgtttatc ggcacctcgt ctgttactgc ctatattgaa
    17581 agtacttctg gtgtggcagt cggtggtcgc acggggctga ctgcggttgt ggttggcgtt
    17641 atgttcctgt tagttatgtt cttctcaccg ctggtggcga tggttcctcc ttacgcaacc
    17701 gccggagcgt taatctttgt tggcgtgctg atgacttcga gcctggcgcg cgttaactgg
    17761 gatgatttta ccgaatcggt gcctgcgttt attaccacgg tgatgatgcc ctttactttc
    17821 tcgatcaccg aagggattgc actcggcttt atgtcgtact gcatcatgaa agtatgcacc
    17881 gggcgctggc gcgatctgaa cctgtgtgtg gtggtggtcg cagctctgtt tgcactgaag
    17941 attattctgg tggattagta actggcgagg gtttccagat atcatgagtt ctgattacgc
    18001 aggagaactc atgatctgga taatgctcgc cacgctggcg gtagtgtttg tggttggttt
    18061 tcgggtgctg acatccgggg ccagaaaagc gattcgccgt ctcagcgatc ggctgaacat
    18121 cgatgtcgta cccgtggagt cgatggtcga tcaaatggga aagtcagctg gtgacgaatt
    18181 tttacgttat ttgcatcgtc cggatgagtc gcacctgcaa aacgccgcgc aggtgttgct
    18241 catctggcaa attgtcattg tcgatggtag cgaacagaac ctgctgcaat ggcatcggat
    18301 tttacaaaaa gctcgcctcg ccgcgccgat taccgacgct caggtcaggc tggcgctagg
    18361 ttttctgcgc gaaaccgaac ctgaaatgca ggatattaat gcttttcaga tgcgctataa
    18421 cgcgttcttt cagcctgccg agggcgttca ctggttgcat tgaaaccgtt tcgtcttatc
    18481 agcctacaga ccgcgcctga cctgtcgggt ggataagacg ttcacgccgc atccggtaat
    18541 ctttgccctg ttccgtttcg atcttaactc cagctcaagc aacaccgtaa aagttgcaaa
    18601 aacgttaaat gcgtcacaca tttcaatgtt aaagtggtcg gcttttcccc tgaaacatgc
    18661 cacgggtaac accatgagtg aatttattgc cgaaaaccgc ggcgcggatg ccatcacccg
    18721 accgaactgg tcagccgttt tctcggtggc gttttgtgtc gcctgtctga ttatcgttga
    18781 gtttttgccc gtcagtttgt tgacgccaat ggcccaggat ttaggcattt cggaaggggt
    18841 tgccgggcaa tcggtgaccg tgaccgcctt tgtggcaatg tttgccagtt tgtttattac
    18901 ccagacaatt caggctactg accgccgcta cgttgttatt ttgtttgccg ttttgctgac
    18961 gctctcctgc ttgctggttt cctttgctaa ctcattcagt ttgcttttaa tcggtcgtgc
    19021 ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg gcgtcgctga ccatgcgtct
    19081 ggtgccgccg cgtacggtgc cgaaggcact gtcggtgatc ttcggcgcgg tttctattgc
    19141 gctggtgatt gccgcgccgt tgggcagttt tttaggcgag cttatcggtt ggcgcaatgt
    19201 ctttaatgcg gcggcggtga tgggcgtgct gtgtattttc tggattatca aatcattgcc
    19261 ttcactgcca ggcgaaccct cgcatcagaa acaaaatact ttccgcttat tacaacgtcc
    19321 gggtgtgatg gcagggatga tcgccatctt catgtctttc gccgggcagt ttgctttctt
    19381 cacgtatatt cgcccggtgt atatgaacct ggcgggattc ggcgtggatg gcttaacgct
    19441 ggtgctgttg agttttggta tcgccagctt tattggtacg tcgctttcgt cgttcattct
    19501 taaacgttcg gtaaaactgg ccttagcagg cgcgccgtta atactggctg tgagtgcgtt
    19561 ggtgctgacg ttgtggggaa gcgataaaat cgttgctacc ggcgtggcga ttatctgggg
    19621 gctaactttt gcattggttc ccgtcggctg gtcaacgtgg atcacccgct cgctggccga
    19681 tcaggcagaa aaagccgggt ctattcaggt ggcggttatt cagcttgcta atacctgtgg
    19741 cgcggcaatc ggcggttatg cgctggataa tattggtctg acttcgccgt tgatgttgtc
    19801 cggcacattg atgttgctga ctgcattgtt ggttactgca aaggtgaaaa tgaagaaatc
    19861 ctgatatcag gtaaagaagc gttggtcagt agttgttgct gttcttacat atccgggcat
    19921 tctaccttac ccaacgcttc ccaataattg ctctgattat tacgttccat cgccacgatg
    19981 gcctcgcgta tatgttgttt attgttttca tctgacaaca ttaacttttc ccacgcttca
    20041 tctgataatg acgcctgtgg cgtgcaaatt ttacgtaaat cttgtaatac cgtggttttc
    20101 tcttttgcgg actgcagtga gctaaaaggc gactcggcgt atacgatgcc tggcatagcg
    20161 aaaaaagtga aaataagcag tagtgtcgtt ggtttcatgg attcaacctt ttacaaatgt
    20221 gtatcgaatg aagcataagt gtagtttgct ttcatcgctc tcatcatgct gtacgaaacg
    20281 cgcttgcgtg gtcagtaaga agtgccagac tttatattcc acttttattc ctttttattc
    20341 ttatcgatag cgtttcgttt tttaaaccgc agcgacctta ccgctatagt caggtaatca
    20401 ttaataaaag gataaaaaaa tgaaactgac aacacatcat ctacggacag gggccgcatt
    20461 attgctggcc ggaattctgc tggcaggttg cgaccagagt agcagcgatg caaaacacat
    20521 taaagttggc gttataaatg gcgcagaaca agatgtcgcg gaagtcgcta aaaaggtggc
    20581 aaaagagaaa tatggcctcg atgttgaact ggtaggattt agcggttcgt tgctaccgaa
    20641 cgatgcgaca aatcatggcg aacttgatgc gaatgtcttc cagcatcgac cctttcttga
    20701 acaagataat caggcgcatg gttataaact ggtagcggtg ggaaatacgt ttgtcttccc
    20761 tatggcgggt tattccaaaa aaatcaaaac cgtggcgcaa ataaaagaag gcgcgacagt
    20821 ggcgatcccc aacgacccga ccaaccttgg tcgcgcactt ttactgttgc aaaaagagaa
    20881 gctaattact ctgaaagagg gaaaagggtt attacctacc gcgctggata taaccgataa
    20941 tcctcgtcat ttgcagatta tggaactcga gggggcgcag ctcccgcgcg tactggatga
    21001 tcccaaagtt gatgtagcga ttatcagcac cacttacatt cagcagacag ggctttctcc
    21061 ggtgcacgac agcgtattta ttgaagataa gaattcgccg tatgtgaata ttttggtggc
    21121 acgggaagat aataagaatg cagaaaacgt gaaggaattt ctgcaatctt atcaatcacc
    21181 cgaagtcgct aaagcggcag aaaccatttt taacggtggc gcggtgcctg gctggtaatt
    21241 ctcactcatg ccgcgccgga cgatttattt ttctggcgcg gcgacgggaa tccattgaaa
    21301 tcaaaatcac cgatgacaga atcagcagcg ttcccagcca gtccggtaat gtgaacgtga
    21361 tccccagcaa cagcaaagag agtagcgcgc tacttaatgg ttctgcacag ctcaaaatgc
    21421 tggcttttgg accgccaatt aattgtgctc ctttcaggta cagactaaat gtcagggacg
    21481 taccaatgac caccaaataa aaaaacgcca gaatcaaact gccgttaacg acaaagtttg
    21541 ttccttgtct ggcataaaaa ggcaacagaa tcagaccgcc aatcagcata ctccagccga
    21601 cgactggtaa cgtgccatag cgggcaatta gcgttgaggg ataggtggta tagaatgcag
    21661 cagcaaatgc cgaggcaatg ccccagaaca acgcggcagg agagatcgat aatgacgtcg
    21721 gattaccgtg tgtcaccaat aaaaaagtcc cgaccagcga tgtcaaaata gcgcagaaaa
    21781 ccagaatgcc cgggcgcgat ttacgcacca gtgagaacca ggcgacgata atcgtcggtg
    21841 agaggaattg cagcaccgtt gccgtggctg cgttcgattt ttcgatggtt agcaaaaaag
    21901 tgagctgtac agttagcgcg ccaaccacgg aaaaaatcag caggctaatg gcatctttat
    21961 gattgttaat aatagaaaag attttatcgc catgaacaaa tgacagcgtc agtagaatca
    22021 aaccggcgaa tatcaaacgc gtcatagtca aaaactgcga cgacatctgg ctttgctcca
    22081 tgatgtattg cgcgcagacc cctgaacttc cccacaacac ggcggcaatc agaacgttca
    22141 gcatcccctt tctggtggaa cccatttttc cctctgcata ctgttttgtc gtttatgtat
    22201 gcaggcatga tagcaaaaat ggcgaggatg gctctgtttg cgccacgccg atttttctta
    22261 gcccagactg gtgagctaaa tatctttaat aaacagcagg caaatcatcg caatacccaa
    22321 catccctatc gccagccaga acaacgcatg gtagctccag atttcaacca taattccgtc
    22381 aacagagccg gcgataatcc agccgacgcg tgaagtattt gcatataacg ttgtcgcaga
    22441 gcctattttt tcaggcatca agtcctgaaa ataaagcatg ccgataccac agagaatacc
    22501 aaggaagatg gcattaagaa tttgcaattc cagctcaacc gccggagtcg tcgccatgag
    22561 tacgctggcg taaaaacaca ttccactcac gatagcaatg agcattaata gtcgcttgcc
    22621 aatacgtttc atgtaatagc ctgcgattaa catcatcgga atttccagac cggcagcgat
    22681 accaatcatt tctccagcca gtttatcggt tagatgcagt tcatcaataa taaatagcgg
    22741 catatttatc atgtagagat tattcgccgc ccacatcatt gaacagacca caaaaagtag
    22801 ccgcgtatcc cgcttcctgt gggtggaggg taaaatttct acgggttggg taacgacagg
    22861 aatatttctt tgtattgatg gcaaaaacaa ccagactatc agtccgcaaa caacaaatgc
    22921 gatggctgcg gtgagataca tcactttaaa actaaatccc attgccagtt cataagcgag
    22981 cggtggcccg ataacccagg caagcgagat ctgagcacgt aaaaatgtac tgaacatgac
    23041 cgtctcacgg cctgttctgt cggcgtgttc acgggcgagg gcgaacattt gcgggtttgc
    23101 ggtggaagca aaactactca gaagtacgcc cgttgagagg agaatgaagt agttgcgatt
    23161 ccacgcaaaa agcgtgcagg ccagcactcc aaataagcaa catagcagaa tcagtaattt
    23221 acggtcgcct tgtttatcgg agtgccttgc cagaaattga ctgaccagaa ttcccataat
    23281 agcgctaccg gtgaagaaaa aacctaccat tataggacgg gctttcagtt catctgcgag
    23341 gaatatactt agggtaggag tctgaagagc gcccgcaata cccgtcagaa aggcgacaag
    23401 taaaaatgcc gcggcagtga gatcaagtat ttttgatgga gtggtagccg ttttttgcat
    23461 tcttctgaat caccgggcga aaaaatataa ctgagttaca aataagtacc agtcgaatga
    23521 gtattgtatt aagaattttt gagtatcgct gatacaacca gaaagcaatg ctagcgcaat
    23581 attatagggc cagcaattga
//
LOCUS       DAFAVF010000060        22782 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFAVF010000060 DAFAVF010000000
VERSION     DAFAVF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.060"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..400)
                     /gene="pdxI"
                     /locus_tag="LGK42_002330"
     CDS             complement(<1..400)
                     /gene="pdxI"
                     /locus_tag="LGK42_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBK2658573.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGH"
     gene            complement(631..1221)
                     /gene="paaY"
                     /locus_tag="LGK42_002331"
     CDS             complement(631..1221)
                     /gene="paaY"
                     /locus_tag="LGK42_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HBK2658574.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(1203..2153)
                     /gene="paaX"
                     /locus_tag="LGK42_002332"
     CDS             complement(1203..2153)
                     /gene="paaX"
                     /locus_tag="LGK42_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HBK2658575.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(2254..3567)
                     /gene="paaF"
                     /locus_tag="LGK42_002333"
     CDS             complement(2254..3567)
                     /gene="paaF"
                     /locus_tag="LGK42_002333"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HBK2658576.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(3594..4799)
                     /gene="paaJ"
                     /locus_tag="LGK42_002334"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(3594..4799)
                     /gene="paaJ"
                     /locus_tag="LGK42_002334"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HBK2658577.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4799..5221)
                     /gene="paaI"
                     /locus_tag="LGK42_002335"
     CDS             complement(4799..5221)
                     /gene="paaI"
                     /locus_tag="LGK42_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HBK2658578.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(5211..6638)
                     /gene="paaC"
                     /locus_tag="LGK42_002336"
     CDS             complement(5211..6638)
                     /gene="paaC"
                     /locus_tag="LGK42_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HBK2658579.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(6640..7428)
                     /gene="paaB"
                     /locus_tag="LGK42_002337"
     CDS             complement(6640..7428)
                     /gene="paaB"
                     /locus_tag="LGK42_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HBK2658580.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(7428..8195)
                     /locus_tag="LGK42_002338"
     CDS             complement(7428..8195)
                     /locus_tag="LGK42_002338"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HBK2658581.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(8192..9262)
                     /gene="paaK"
                     /locus_tag="LGK42_002339"
     CDS             complement(8192..9262)
                     /gene="paaK"
                     /locus_tag="LGK42_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HBK2658582.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(9270..9767)
                     /gene="paaD"
                     /locus_tag="LGK42_002340"
     CDS             complement(9270..9767)
                     /gene="paaD"
                     /locus_tag="LGK42_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HBK2658583.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9782..10528)
                     /gene="paaC"
                     /locus_tag="LGK42_002341"
     CDS             complement(9782..10528)
                     /gene="paaC"
                     /locus_tag="LGK42_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HBK2658584.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(10537..10824)
                     /gene="paaB"
                     /locus_tag="LGK42_002342"
     CDS             complement(10537..10824)
                     /gene="paaB"
                     /locus_tag="LGK42_002342"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HBK2658585.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(10836..11765)
                     /gene="paaA"
                     /locus_tag="LGK42_002343"
     CDS             complement(10836..11765)
                     /gene="paaA"
                     /locus_tag="LGK42_002343"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HBK2658586.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            12050..14095
                     /gene="paaZ"
                     /locus_tag="LGK42_002344"
     CDS             12050..14095
                     /gene="paaZ"
                     /locus_tag="LGK42_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HBK2658587.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDVLVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            14343..16616
                     /gene="tynA"
                     /locus_tag="LGK42_002345"
     CDS             14343..16616
                     /gene="tynA"
                     /locus_tag="LGK42_002345"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HBK2658588.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(16674..18173)
                     /gene="feaB"
                     /locus_tag="LGK42_002346"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(16674..18173)
                     /gene="feaB"
                     /locus_tag="LGK42_002346"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HBK2658589.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            18409..19314
                     /gene="feaR"
                     /locus_tag="LGK42_002347"
     CDS             18409..19314
                     /gene="feaR"
                     /locus_tag="LGK42_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBK2658590.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(19486..19815)
                     /locus_tag="LGK42_002348"
     CDS             complement(19486..19815)
                     /locus_tag="LGK42_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBK2658591.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(19820..20005)
                     /locus_tag="LGK42_002349"
     CDS             complement(19820..20005)
                     /locus_tag="LGK42_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBK2658592.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(20002..22641)
                     /locus_tag="LGK42_002350"
     CDS             complement(20002..22641)
                     /locus_tag="LGK42_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBK2658593.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT         5505 a         6207 c         5560 g         5510 t
ORIGIN      
        1 catgaccatc tcccatcata acgcgcaggt taaccacatc cagtacgtcc aggccgagat
       61 tacgtagatt atcgtgcacc gctttttgca actctgccgg agaaaaggcg ggcaaccagg
      121 aagcgtcctc tccacgccgc gcaccaattt tagtgacaat tgtcaggtcg tcagagtaag
      181 gataaagcgc ttcgcggata atctgattgg tgacgtgcgg accataaaag tcgctggtat
      241 caatgtgatt gacgcccaat gccagcgcct cgcgcagcac agttatagcg acgtggcgat
      301 ctcttggggg accaaaaacc ccaggacctg ccagttgcat cgcgccataa ccaagacggt
      361 taacggattt tgtaccgaga gtaaatgtat tgctgctcat cgcaccctgc cctgtaataa
      421 aacggattaa gaaaaatcag tatagaaacg aaaagagata aataatgcga gggcgtaaac
      481 aagattactg gatgagttgc tctattgtcc tgaaaaacac aaaacaaaac gcgtaaagcg
      541 ccgcatccgg caataaaaaa accgcaacct gtatcagcgt tgcggtttag gtatcacatg
      601 ctaaaaatta gaatgttcag tatctggacg ttactgtttc ggtcgcagat tctcatcaaa
      661 taccagacgt ttcctgttcg gttcaacttc ccgcaatggc tcaacttgat gcaacgtctg
      721 cttacaacga gtcaccagca cctgatactc atgcgtaccc tgctttttcc atgccagctc
      781 ctgctcactg agttcacgaa tcgctttcgc cgggctgccg acaatcaggt aattggcagg
      841 catttctgct ttggctttca caaatgcgga tgcgccaaca atgctgttct cgccaatcac
      901 cgcgccgtcc atcactaccg cgttcattcc cactaatgca ttgcggcgga taatgcaacc
      961 atgaaggata gcgctatgac caatatgtcc atcttcttcc acgacagtat cctgctcggg
     1021 aaaaccgtgc ataacgcaat tatcctgaat gttcgcgcca tctttcacca cgatacgacc
     1081 aaaatcgcca cgcaggctgg catttggccc aacgtaaacg cccttgccta gaataacatc
     1141 gccgatcaat accgctgtcg gatggacaaa actctcttct ggcacaaccg gagtcagacc
     1201 atctatctgg taaattggca tatcgcctcc tgttcaatat tcaaaccgcc aaaacgttga
     1261 aaatacaggc ttcccggcgc aggcagttca ccgaccgagg tttcaccttt ttcactaacg
     1321 aacgctaaag cagcaggcgc tacgcgctga taaatgttga tacacagctg acgcgccgta
     1381 tgccctgccc agtgcgccgg aagcaactcc tctggcaaca atgggtcttt aaggacgaca
     1441 cggcgataaa aatggatcag taaaagctga atatgaaatg cacgctccgg ggttaactcg
     1501 tctgctgcct cttttaacag cggcaccagc gggcggaatg actgaataaa ggtttcgtac
     1561 atggcatttt gttcagttaa atgccagcac tcttccactc tggcacgcag tgctgcacga
     1621 gaaagcgcca gtggtatttg cgcttcaaaa caaatcacgt tatccgccac ccccgcttca
     1681 tgcaaaagtg tctgtacatc cgccagtttt tgcgacggcg atgccatcag gctgggtgcc
     1741 agtgcgccaa aaccttgcca gatcaactgc tttttgacat cagccagcgt tgatttatct
     1801 aaaccttccg agagcaacag gagccattta ccatcccatg caggttgctc tgcgcgataa
     1861 attttacttt ctgcccgtcg cgtcaggcgc aagcctttat cactgaggct atagaaactg
     1921 cgtcgcccga tgcgggaaac atccagccag ccttctttat taagacgaaa caaagcggtg
     1981 cgcacgaaac gctcaccaaa tcccagccct tccagcaaag cagccagact accaagccag
     2041 atttcaccac cacgatggga aagcgaatca ccatacagag aggagatcaa agatgtgcca
     2101 ctgaccggaa cagcgtttac agcatgttgg ataaaagtat caagtttact cattcgcttc
     2161 agtctgttgt tattgttgtg atttatgaat catagcacag gtcgtatcga cttctggccc
     2221 tggcgcttta cctcaccagg gccagacgac acgtcaggca ccaacaatat tgcgcagatc
     2281 aaacacccga cacgccttgc cttctgaacg cgggatactg ccacagttaa cgatcatcac
     2341 atcggtagag atccccacca tcgacttaat ccgatggcgc agctggtggc acacctggca
     2401 acgctgctca tgcgtcaatg tcaggctgct ttctttcaac tccactttca cagaaagtga
     2461 atcaagatgc ccccggcggt tcacctccag ttggtaatgg ggcgataaat gttcgaattt
     2521 gacaatctct tcttccagtt gtgacgggaa gacatttaca ccgcgaatga tcaacatgtc
     2581 atcgctgcgt ccgctgatgc gatccatgcg gcgcatagtc cgcgccgttc ctggtaacag
     2641 tcgggttaaa tcacgcgtgc ggtaacgaat gaccggcaat gcttctttgg tcagcgtggt
     2701 gaataacagt tcgccatgtt caccatcggc aagcggtgtg ccgtcatgag gattaacaat
     2761 ttcaggatag aaatgatctt cccaaatggt tgggccgtcg gtagtttcca gacactccat
     2821 cgccaccccc ggccccatca cttctgacag gccataaata tccagtgcgg tgatccccag
     2881 acgccgttca atctctttac gcatggcctg tgtccacggc tcagcaccaa acactccaac
     2941 ccgcagcgag caaccgctgg catcaccgcc caactgccgc tccagctctt caatcaggtt
     3001 aaggcaataa gatggcgtaa ccatgatcat atctggttga aaatcgcgga tcagttgcgc
     3061 ttgtttttcc gtctgcccac cagacatcgg gatcaccgta gcgcccagac gttcggcacc
     3121 atagtgcgcg cccagcccac cagtaaacag gccgtaacca taggcaacat gaattttgtc
     3181 tttgggcgtg ccacctgccg cacgcaagga acgcgctaca atattggccc agttatcaat
     3241 atcgttttgc gtatagccga caactgtcgg tttacctgtg gttcccgaag aagcatgaat
     3301 gcgcaccact tgttccatcg gcacggcaaa ggtgtcgaag ggatagttgt cgcgcagatc
     3361 ctgtttggtg gtacagggaa atttacgcag atctgaaagt tccctgaaat catcaggatg
     3421 tacgcctgct gcgtcaaatt tgcgccgata catcgggaca ttttcatacg cgtgtttgag
     3481 cgtccatttc agacgttgtg tttgcaacgc ctgtaactca tcaacagacg cggtttcaat
     3541 cgggtcaagc tttgtattgg ttatcattgt agggtactcg caggttgata tgctcaaaca
     3601 cgctccagaa tcatggcgat gccctgaccg acaccgatgc acatggtgca caatgcgtaa
     3661 cgaccgttac gccgatgcag ctcatggctg gcagccagtg ccaggcgggc accactcatt
     3721 cccaacggat ggcctaaggc gatagcgcct ccgttggggt taacatgtgg ggcatcatca
     3781 ggcagcccca attcgcgtag tacacccaac gcctgggccg cgaacgcttc gttcagttca
     3841 atcacgtcca tatcgtgaat actcagcccc gcgcgttcca gcacccggcg agttgcaggc
     3901 accggaccaa gccccatcag gcgcggttcc accccggcgg ttgccatggc tacgattcgc
     3961 gcccgcggtg tcagtccttg cgctgctgcc atctgttcac tggcaataat caacgcagcg
     4021 gctccgtcat tcaccccgga ggcattgcct gcggtaatca ccccattggc acgaaatggt
     4081 gcttttaacc cacgtaactg ttccagcgtc gtttccgggc gcagatgctc atcatgttgt
     4141 atttctgtta caacgccttt cttgtttttc aacacaaccg gaacaatctc ctcagccaga
     4201 atgcctgagg attgcgcttt tgccgtacgt tgctgactgc gtagcgcaaa actatcttga
     4261 tcttctcgcg atatttttaa cagttcagct acattctctg ccgtttccgg catgctgtca
     4321 gttccaaatt gttgagccat gagcgggttc acaaatcgcc agccaatagt ggtatcaaac
     4381 atctcagcct gacgagaaaa tgcactggtt gccttgccca taacaaacgg tgcccgagac
     4441 attgactcca cgccaccagc gatcagcaaa tcgccatcgc ccgctttaat cgcccgtgcg
     4501 gcgaacccca gtgcgtccag cccggaacca cacaagcggt taatggttgt gccggaaaca
     4561 ctctgcggca gccccgccag taacgtcgcc atccgggcta cgttacggtt gtcttctccc
     4621 gcctgattag cacagccgag gatcacatca tcgatacact ccgcatcgag acgcgggttt
     4681 cgcaccagca gctcccgcaa agggatagca gccagatcgt cagcccgaac acctgataat
     4741 gccccaccat agcggccaat tggcgtacga attccgtcac agataaaggc ttcacgcatc
     4801 aggcttctcc tgtaatggtg ccgccgatgc ggtgagattt accgcgaaac agcgcaaccg
     4861 ttttttgttg ttggttaaca atttcgatgt cgtaaacacc ggtttgcttg ccctgatgac
     4921 gtacctgcgc agtagcagtt aaggtgtctc cggcaaagcc tggacgcaaa aaatcaatcg
     4981 tgcaggcaga agcgacggct gccagcccct ggctattgca ggcgtaggca aaggcagtat
     5041 cagccagtga aaatagctgc ccgccgtggc aactttgatg accgttaagc atttgtgcag
     5101 tgacggtcat ggtcactaca gcaaagcctt catccattga gataatgtcg atgccaagcg
     5161 ctttggcgca ggcatcgttc tcatacattg catgggcatt ttgccaggcc ttatgactca
     5221 taaccgctct ccagaagcgc tcgttggcgc agcaatgaac atgggcgata gcgttcttcg
     5281 ccatagtgat gttgtagatt ttccaagagc cttaatattc gctgccagcc aagttgcgct
     5341 ccccaggcaa gcgggccata tggataattc accccaagac gcatggcggt atcgatatcc
     5401 tgttcagagg ccacgccttt ttgcagcgca tcaagggctt cattgatgat cattgctacc
     5461 gttcgccaaa tcagcattcc tgggtaatct gcaatttgca gcactgtttt gccctgctgt
     5521 tgcaggtaat aaatggcctt gcgggtcgct gagtccgggt tcaccgctgc cgcagcaatg
     5581 gtcaccacct tgcccgccat tttgtcgacc actaccaccg ggcgtgccag tcgtattgcc
     5641 agcgcctgtg ccgtctcgcc ttgtgtctca atcaataaaa catcgtcaat ttccgtgacg
     5701 ccgtcacttt tcttttctac tttcattggg ctaaaactgt cgcttaccgc ttccaggcca
     5761 acaactgcct cacgttccgc gcgccagtcg tacacgccca gcccactttt cttgcccaac
     5821 cgtccaccaa tcaccagttc ctgttgcacc agcgaaggta aaaaacgacg ctcctgccag
     5881 aaagcgttaa acaccgaaca ggtgacagca aaattgacgt cctgaccaat cagatcggtt
     5941 aattccagcg gccccatcgg gaaaccagcg ccatcgcgaa gtgcagcgtc aatcacttct
     6001 ggtgcagcaa cctgctcttc cagtgcccgc caggcctcgg aataataagg acgcgccaca
     6061 cggttaacga taaatccggg ggtcgaatga cagcgcactg gctgcttacc ccaactcaat
     6121 gtcagttcac acaactgctc gacaacttcc gctgccgttg ccagcccact gaccacctcc
     6181 accaacttca tcaccggtgc cgggttaaaa aaatgcagcc ccgcaacacg ttcaggattt
     6241 tttacctccg cagcaatcgc ggttatagag attgacgaag tgttagtggt caatagcgtt
     6301 tgtggcgggc aaacttccgc cagttgtgca aagagcgctt ttttaacttc cagacgttca
     6361 gacgccgctt caataaccag attcgctgct gccagcgcgt gaatatcggt caccgggatc
     6421 aggcgtttca atgtgcgttc acaggtttca gcagtcagtt ttccccgcgt cacgcgtgaa
     6481 tttagccgcg cgtgtatccc gtcgattgcg cgggtcagcg cttcagcaga aatgtcatac
     6541 agtaaaacct gatgtccatg actggcggca acttcagcaa tgcctgcccc catggtgccg
     6601 ctcccaatca ctgccacagt ttgcacattt atcatcatgc tatttccccg tgaactgcgg
     6661 tgagcgttta gccaggaacg cactgacacc ttcacgataa tctgcgctcc gtccggcaag
     6721 tcgctgatag tcacgttcca gatccagttg cgtatcgagc gtattggttt cagcgctatt
     6781 tatcgcttgc ttgataagtc caagaccaaa tgtcggttgt gtcgccagat gccgtgccag
     6841 ctgttgcgcg gtatctgcca gcgtttcatc atcaacaacc tgccagatca tcccccattc
     6901 gtgcgcctgt tcagcactca gttgattccc cagcagtgcc agccccatgg cgcgcgctcg
     6961 cccagcaacg cgtggcagta accaggttcc accgcaatcg ggtactaaac ctaacttact
     7021 aaacgccatg acgaattttg ctgaacgggc agcaataacg atgtcgcatc ccagtgccag
     7081 tgttgcgcca gcccctgccg ccacgccatt gactgcacag atcaccggtt ttggcagttt
     7141 tgccaggcga cgtaccagtg ggttatagaa acgttcaact gacatcccta aatccggtgc
     7201 ggggcctgtg ggatcaacgt tacgatcgtt aagatcctga ccagcacaaa atccgcgccc
     7261 ggcacctgta agtaacaggc aacggatagt gtcgtcgcgc tcgacctgtt tcaggcactc
     7321 tgccagttgt gcgtgcatct catcattaaa actgttcagg cgttccgggc ggttgagcgt
     7381 tagtgtcatc acgccctttt ctacatgact gaggatgaat tccatcatta gcgtccttta
     7441 aagtcgggcg tgcgtttttg caagaaagcg gagatgcctt catgacgatc ttctgttgcc
     7501 gccagcaagg tgaataactg tcgctcctgg gcaagtccgg cttgcaaagc cacttcctgc
     7561 gactggcgca gcgcttgctt tgccgcttgt aaggccagcg gcgagtgacg tgccattttc
     7621 gacgccagct gtaaggcgta ttcgagggtt aaatcgctgg ggaagacgtc gctaaccagc
     7681 ccggcctgct gtgcttgctg agcggtgata ctttctccgc tcagcaccat tttgctggct
     7741 aacgatttac cgacactacg gattaaacgt tgcgttcctc ctgcgcctgg catgatgccg
     7801 agagtgattt ccggcaaacc aaaacgcgcg ttctctccgg caaccaccac atcgcacaac
     7861 aatgccagtt cgcaacccgc accaagcgcg taaccgttga cggctgcgat gagaggtttg
     7921 ttgaaggcct gcaatcgcgc ccatagctgc gggcgtgtat cgtttaaggt ggccgcgaga
     7981 tctttttctg ccatttcgtt gagatcggcc ccagcggcaa aaaagcgtgc attaccggta
     8041 atcacacaga ccgaaatgct gctatcggta gccgcagctt ccagttcatt taccagttgc
     8101 gtcagaaggg cattatttag cgcattacgt gcggcgggac ggttaagggt cagcaacaat
     8161 acttgttgct gacggctgac gatcagttcg ctcatgccat ccccttcgcg tcaaagtcaa
     8221 ccaccacatc gctggtcagc ggcagtgcct ggcaactcaa cacatagcct gcggccagtt
     8281 catccggttc cagactgtaa ttggtttcca tcgccacttt gccacgcagc actttgcatt
     8341 tgcaggtcgc acagacgccg cctttgcagg catagggcag atcagccccc tggcgcaatg
     8401 ccgcatcgag aatgctttca tcgtcggcat taagcacgat ttcccgatcc cgcccatcct
     8461 gacgtacagt cactttttgt ccgtcacttt gcacgttaac gctacgtttg acgcgcgtgc
     8521 caggcgtatt aaaccgctcc agatgaatgg ttttatctgg cattcccagt gcttttaagg
     8581 cggcttccgt ctcatccatc atcgccgccg gaccacaaat aaatgcctca tcataaagac
     8641 gaaaattaat gagcgaggcc ccaagtgact gtaatttttc accgtcaata cgcccgtgaa
     8701 gcagatcgct gtcgagggtt tcctgactga aaatgcacaa caactgtaaa cgctgaggat
     8761 atttgtcttt caggtctgcc agtgcctggc gaaacatcat gctctggctg gtacggttac
     8821 cgtagatcag ggtgaactga ctttcaggct cggtttgtaa agtggtggcg ataatcgcca
     8881 gcattggcgt aatacctgat cctgctgcaa tagccagata gcggccctgg cgttcggcct
     8941 gtggctgata gccgaaatgc ccctgcggca ccatgacctc cagcgtcata ccctggcgga
     9001 tgtgttcgcg ggcatagcgg gagaaacgtc cgccttcaat ggctttcacc gccacactaa
     9061 tttcgccagg cagatagctg cggcaaatgg agtaacaacg gcgtaattct tcaccatcaa
     9121 agctggcttt taaggtcaaa tgttgaccgg ggcgaaagcg atacgcctcc tgcaaaggct
     9181 ggggcaccgc aaaggtaatg gtcaccgcat cacgggtttc cggctccact tttgccaccg
     9241 ttaaggaatg aaacgttgtc atggcatcct caaatacatt tgaaataatc gaaaggttcg
     9301 cggcaactat cgcagcgata caatgctttg caggccgtgg aaccaaattc actgataagt
     9361 gtggtatgga cgctggcgca gcgcgggcaa cgtacttctg gcggcaaatg ggcatggcaa
     9421 ctgtgtccgg cgggcgggct aatgccatac tgtcgcagac gctcacgggc atcgggggtc
     9481 atccagtcgg tggtccatgc cgggtcgagt tgcagcacaa cctgaacggg ggtaaacccg
     9541 tgggttgtca ttgcctcacg tatcgcgcca atcaaatgtt ccgttgccgg acaaccggaa
     9601 taagtcggcg taaagccgat cacccatcct tctcccatct gtgtcacatt acgcaccatg
     9661 cctaaatcgg taatggtcag caccgggatc tccgggtccg ggatctggct gagtagcgcc
     9721 catatctcat gaacctgtgg cggtgcaatg gtggccagac gttgcatacc catctcctct
     9781 gttaccattg ctgaccgggc aagacacgct ggagatactg catttctgcc agcatgggtc
     9841 caaggtgttc ggtatgcagt ccttttttac cgccagtgcg atacgcctgc tcttgcggta
     9901 cgttcaatgt ggcttcgttg atcccggcaa aaacttcggc ttcccacgct gcgcgtaaag
     9961 tgcgtggatc aaccgcaata ccctcttcac tcagtgcaat atcaatctcg tcggcatcga
    10021 acagttcggc ggtaaaacgc cacaacttgt tgatggcctg ctgcatcttt tgccctgata
    10081 cgtcagtacc attgcccagt cgctccagcc agccacgact aaaacgcagg tgataacgcg
    10141 cttctttaat tgctttggca gaaatcgccg ccagttgcgg atcacggctt tccatcagac
    10201 gggtaaagag cgccacatgc catgcatcga tgaaatactg gcgtgcaatg gtgtcggcaa
    10261 aattgccgtt tggttgttca accaacaata agttgctgaa ctggcgctcg tctcgggtga
    10321 aggccagggt atcttcatca ccttctcccg ctaattcagc ggcatacgat aagaagttac
    10381 gtgcctgacc taataaatcg aggccaatgt ttgccagtgc gagatcgatt tccagttccg
    10441 gtgcgtgacc gcaccattcc cccaaccgct gggagagcac cagacagtta tcgcccaggc
    10501 gcaaggtgta agccgttaac tgattcattt ccgacctcac atgtgctcaa tgccatcagg
    10561 gatggtgtaa aacgttggat ggcgatagac cttgctttca gccggatcaa aaaattcacc
    10621 gcgttcttcc ggttgcgagg caacaatttc actcgccttc accacccaaa ttgaacatcc
    10681 ttcgctacga cgggtgtaag catcacgggc attttccagt gccatccgct catcggcagc
    10741 atgtaaactg ccaacatggc ggtgtgataa gccctgtttg ccacgcacga aaacttcgta
    10801 taacggccag taaacattac tcattttgaa tctccttatg cgaccttacg ggcaagttgt
    10861 ttttgtgcat gggccagcgc tgcttcccgt acccaggtac cttcttccca ggctttacgt
    10921 ttggcgtcga gcctctcctg attacaaatt ccgcgaccgt taattacttc gttaaactcc
    10981 tgccagtcga tctcaccaaa gcggtagtga ccgctttcag tgtcaaaatg cagatccggg
    11041 tcaggaacgg tcataccgag catttcaacc tgtggaacgg tgttatccac gaaacgctgg
    11101 cggagttcgt cgttggtgaa acgtttgatt ttccaggcga gacttctggc gctgtttggc
    11161 gagttatcat cgtttggccc gaacatcatt aaggctggcc accagaaacg gttaatggcg
    11221 tcttgcaaca tctgcttttg agcttcgctg ccctgcgcca gtgccatgca ggcttcaaac
    11281 ccctgacgct ggtgaaaact ctcttctttg cagattttca ccattgctct ggcatacggg
    11341 ccataagaag tgcggcataa cgccacctga ttgacgatcg ctgcgccatc caccagccag
    11401 ccgataacac cgatatcagc ccaactcaat gtcggataat taaagatgga ggaatatttc
    11461 atccgtccgt cgagcatttt ttggtagatg tcttctctgg cgcagcccag tgtttcggcg
    11521 gcgctataga gatacagacc atgaccggct tcatcctgca ctttggccaa cagaatggct
    11581 ttacgccgca aggttggtgc gcgagtgatc cagttacctt caggcaacat accaacaatc
    11641 tcggagtgcg catgctgccc aatctggcgg atcagtgtct ttcggtaagc atcgggcatc
    11701 cagtcctgcg gctcgatagc cgtttcctgg gctatccgtt gctcaaagcg ttcttcttgg
    11761 gtcacgtcat cacctttacg attccagttt tcgtgaatta attaaaaaca cgaatcacgt
    11821 tgttttatga aagttacaca atggttaact aaaatcacaa attttgtatg cttgaactgt
    11881 gacggatttc gcaccctatt tttataaatc attatttatc agtgcattaa tgttttccgc
    11941 agttgctata cagatcgcat agttaacatt tcgttaaaag aacccttgct ttttatgatt
    12001 cgcgatttaa ctattagcaa cagaaatgtg aaacatctgg agagtagcga tgcagcagtt
    12061 agccagtttc ttatccggta cctggcagtc tggccggggc cgtagccgtt tgattcacca
    12121 cgctattagc ggcgaggcgt tatgggaagt gaccagtgaa ggtcttgata tggcggctgc
    12181 ccgccagttt gccattgaaa aaggtgcccc cgcccttcgc gctatgacct ttatcgaacg
    12241 tgcggcgatg cttaaagcgg tcgctaaaca tctgctgagt gaaaaagagc gcttctatgc
    12301 tctttctgcg caaacaggcg caacgcgggc agacagttgg gttgatattg aaggtggcat
    12361 tgggacgtta tttacttacg ccagcctcgg tagccgggag ctgcctgacg atacgctgtg
    12421 gccggaagat gaattgatcc ccttatcgaa agaaggtgga tttgccgcgc gccatgtact
    12481 gacctcaaag tcaggcgtgg cagtgcatat taacgccttt aacttcccct gctggggaat
    12541 gctggaaaag ctggcaccaa cgtggctggg cggaatgcca gccatcatca aaccagctac
    12601 cgcgacggcc caactgactc aggcgatggt gaaatcaatt gtcgatagtg gtcttgttcc
    12661 cgaaggcgca attagtctga tctgcggtag tgcgggcgac cttttggatc atctggacag
    12721 ccaggatgtg gtgactttca cggggtcagc ggcgaccgga cagatgctgc gagttcagcc
    12781 aaatatcgtc gccaaatcta tccccttcac tatggaagct gattccctga actgctgcgt
    12841 actgggcgaa gatgtcaccc cggatcaacc ggagtttgcg ctgtttattc gtgaagttgt
    12901 gcgtgagatg accacaaaag ccgggcaaaa atgtacggca atccggcgga ttattgtgcc
    12961 gcaggcattg gttaatgctg tcagtgatgt tctggttgcg cgattacaga aagtcgtggt
    13021 cggtgatcct gctcaggaag gcgtgaaaat gggcgcactg gtaaatgctg agcagcgtgc
    13081 cgatgtgcag gaaaaagtga acatattgct ggctgcagga tgcgagattc gcctcggtgg
    13141 tcaggcggat ttatctgctg cgggtgcctt cttcccgcca accttattgt actgtccgca
    13201 gccggatgaa acaccggcgg tacatgcaac agaagccttt ggccctgtcg caacgctgat
    13261 gccagcacaa aaccagcgac atgctctgca actggcttgt gcaggcggcg gtagccttgc
    13321 gggaacgctg gtgacggctg atccgcaaat tgcgcgtcag tttattaccg acgcggcacg
    13381 tacgcatggg cgaattcaga tcctcaatga ggaatcggca aaagaatcca ccgggcatgg
    13441 ctccccactg ccacaactgt tacatggtgg gcctggtcgc gcaggaggcg gtgaagaatt
    13501 aggtggttta cgagcggtga aacattacat gcagcgaacc gctgttcagg gtagtccgac
    13561 gatgcttgcc gctatcagta aacagtgggt gcgcggtgcg aaagtcgaag aagatcgtat
    13621 tcatccgttc cgcaaatatt ttgaggagct gcaaccaggc gacagcctgt tgactccccg
    13681 ccgcacaatg acagaggccg atattgttaa ctttgcttgc ctcagcggcg atcatttcta
    13741 tgcgcatatg gataagattg ctgctgccga atctattttc ggtgagcgtg tggtgcatgg
    13801 gtattttgtg ctttctgcgg ctgcgggtct gtttgtcgat gccggtgtcg gtccggtcat
    13861 tgctaattat gggctggaaa acttgcgctt tatcgaaccc gtaaagccag gcgataccat
    13921 ccaggtgcgt ctcacctgta agcgcaagac gctgaaaaaa cagcgtagcg cagaagaaaa
    13981 accaacaggt gtggtggaat gggcggtaga ggtattcaat cagcatcaaa ccccggtggc
    14041 gctgtattca attctgacgc tggtggccag gcagcacggt gattttgtcg attaatcggt
    14101 gaatgaaggg caacggcgaa tagttgccct tttatttcac caaattttgt gacgttgtca
    14161 cgttatgcat gatgtgtatc tgtattttca gggcacccac tgtatgaaaa gctggcacac
    14221 ctgccaaaca acctggcagg tgcaggcaat cccctttaca tcagtactga taatgtgaac
    14281 ctgactaaac cgcccacaga gcgcggttgc taacaagaac acaacatctg acgaggttaa
    14341 taatgggaag cccctctctg tattctgccc gtaaaacagc cctggcgttg gcagtcgcct
    14401 taagtttcgc ctggcaagcg ccggtatttg cccacggtgg tgaagcgcat atggtgccaa
    14461 tggataaaac gcttaaagaa tttggtgccg atgtgcagtg ggacgactac gcccagatct
    14521 ttaccctgat taaagatggc gcgtacgtga aagtgaagcc tggtgcgcaa acagcaattg
    14581 ttaatggtca gtctctggca ctgcaagtac cggtagtgat gaaagacaat aaagcctggg
    14641 tttctgacac ctttattaac gatgttttcc agtccgggct ggatcaaacc tttcaggtag
    14701 aaaagcgccc tcacccactt aatgcgctaa ctgcggacga aattaaacag gccgttgaaa
    14761 ttgttaaagc ttccgcggac ttcaaaccca atacccgttt tactgagatc tccctgctac
    14821 cgccagataa agaagctgtc tgggcgtttg cgctggaaaa caaaccggtt gaccagccac
    14881 gcaaagccga cgtcattatg ctcgacggca aacatatcat cgaagcggtg gtggatctgc
    14941 aaaacaacaa actgctctcc tggcaaccca ttaaagacgc ccacggtatg gtgttgctgg
    15001 atgatttcgc cagtgtgcag aacattatta acaacagtga agaatttgcc gctgccgtga
    15061 agaagcgcgg tattactgat gcgaagaaag tgattaccac gccgctgacc gtaggttatt
    15121 tcgatggtaa agatggcctg aaacaagatg cccggttgct caaagtcatc agctatcttg
    15181 atgtcggtga tggcaactac tgggcacatc ccatcgaaaa cctggtggcg gtcgttgatt
    15241 tagaacagaa aaaaatcgtt aagattgaag aaggtgcggt agttccggtg ccaatgaccg
    15301 cacgcccatt tgatggccgt gaccgcgttg ctccggcagt taagcctatg caaatcattg
    15361 agcctgaagg taaaaattac accattactg gcgatatgat tcactggcgg aactgggatt
    15421 ttcacctcag catgaactcg cgcgtcggac ctatgatctc caccgtgact tataacgaca
    15481 atggcaccaa acgcaaagtc atgtacgaag gttctctcgg cggcatgatt gtgccttacg
    15541 gtgatcctga tattggctgg tactttaaag cgtatctgga ctctggtgac tacggtatgg
    15601 gcacgctaac ctcgccaatt gcacgtggca aagatgcacc ctctaacgca gtgctcctga
    15661 atgaaaccat cgccgactac actggcgtac cgatggagat ccctcgtgct atcgcggtat
    15721 ttgaacgtta tgccgggccg gagtataagc atcaggaaat gggccagccc aacgtcagta
    15781 ccgaacgccg ggagttggtg gtgcgctgga tcagtacagt gggtaactat gactacattt
    15841 ttgactggat cttccatgaa aacggcacta ttggcattga tgccggtgct acgggcatcg
    15901 aagcggtgaa aggtgttaaa gcgaaaacca tgcacgatga gacggcgaaa gatgacacgc
    15961 gctacggcac gcttatcgat cacaatatcg tgggtactac acaccaacat atttataatt
    16021 tccgcctcga tctggatgta gatggcgaga ataacagcct ggtggcgatg gacccggtgg
    16081 taaaaccgaa tactgccggt ggcccacgca ccagtaccat gcaagttaat cagtacaaca
    16141 tcggcaatga acaggatgcc gcgcagaagt ttgatccggg cacgattcgt ctgttgagta
    16201 acccgaacaa agagaaccgc atgggcaatc cagtttccta tcaaattatt ccttatgcag
    16261 gtggtactca cccggtagca aaaggtgccc agttcgcgcc ggacgagtgg atctatcatc
    16321 gtttaagctt tatggacaag cagctttggg taacgcgtta tcatcctggc gagcgtttcc
    16381 cggaaggcaa atatccgaac cgttctactc atgacaccgg tcttggacaa tacagtaaag
    16441 ataacgagtc gctggacaac accgacgccg ttgtctggat gaccaccggc accacacatg
    16501 tggcccgcgc cgaagagtgg ccgattatgc cgaccgaatg ggtacatact ctgcttaaac
    16561 catggaactt ctttgacgaa acgccaacgc taggggcgct gaagaaagat aagtgattgt
    16621 ttcagacaaa aaaacggcac caggtgccgt tttttactta tgagcgaacc agattaatac
    16681 cgtacacaca ccgacttagt ttcacaccaa ccgtccagcc agtcggggcc aaaatcacgg
    16741 cccgttcctg actgcttcat cccaccaaac ggtaagttag cgtcaattaa ggtatggctg
    16801 tttacccaca ccgtccctgc ctgtaagcga tcgctatatt ccagagcctg ggagagattt
    16861 tgcgtccaga cactggcagt taagccatat tccgtgtcgt ttgccagttg taacgcctct
    16921 tctccatccg ctactcgcac caggtttacc accggaccaa acacctcttc acgagttaag
    16981 cgcaatttag catcgggatt taccaccagc gttggcgcaa cataataccc ctctccggct
    17041 ggtccattcg acccgcgaat cagctctgct tgctgtgcct gcgcatcgtc gaggaatgaa
    17101 cacactttgt cgcagtgcgc acgagaaacc aaagggttaa tctgtgcaac aggtgacatc
    17161 cccggtccca cttgcaacga ttttaccgcc tgctcaaatc cactaaccag cgtgtcaaac
    17221 aacggcgctt caatataaat tcgcgaactg gcggcgcata cttgcccttg attcaggaag
    17281 cttccggtca tcaagccttc aataacccat tgcggatcag catcttttaa tacaattgcc
    17341 gggtttttac cgcccagttc cagcgttaca cgcgttaagt gatcagcagc agttctggca
    17401 ataccttttc ccgtcgcggt tgaaccggta aaactgattt tcgcaacatg aggatgtgac
    17461 gtcagggccg cgccgcatac agcacctgac ccggtgacca cattaaaaac gccatcaggg
    17521 ataccagcct cgctggccag ttccgccacg cgcaacatcg tcagtggcgt ggtttccgaa
    17581 ggcttaatca cgattgaaca gcctgctgcc agtgctggca tcaccttcca cataccaatc
    17641 atcaacggaa agttccatgg cacaattccc gccactacgc caaccggctc tttacgcgtc
    17701 caggcctgat aacgcgcccc ctggggtaag ggaatcgaca agtccagcgt tttacccgcg
    17761 attttggtcg ttaacccggc ggtataacgc atccagttca gcgtacagcc cacttcaaaa
    17821 gcacgggaaa tggcaattga cttgccttgc tccagggttt ccagttgcgc cagctcctca
    17881 ctgtgctgtt ccaccagatc ggcaaaacgt agcagaatac gttcacgctc tgcgggtaat
    17941 cgcccggccc agcgacgcga gacaaaggcc cgccaggcag acatgactgc gttatctaca
    18001 tccgcttcgt tggcatcagc agtagacgca atttcttgcc cggtggccgg atcaaagatc
    18061 gccaatcgtt tttcactttg tgcggggcct ggacgaccat caatataaag accgtgttga
    18121 cgatcgagaa actgttggac ctggcttaat actgctacat gcggctctgt catcactttt
    18181 ccttattatt tacccagtgt gatttcagtg tacgcagcga caagaaaagt cacttttttc
    18241 gctgtgacat tcgctttgtc agctgtgaca agctccgcaa atcgtgacaa taaccgctta
    18301 tgtctctatg cctgacaaga cttatttcct atagtcaggc tcattagctt cgtttattgc
    18361 aacacaaatg caacaataaa aatacatttc acagagcgaa aacgtgccat gaaccccgca
    18421 gtggataatg agtttcagca atggctttcc caaatcaatc aggtatgcgg aaattttacc
    18481 ggacgcctgc tgactgagcg ttacacgggt gtactggaca cgcattttgc caaaggacta
    18541 aagctgagta ccgtgacaac cagcggggtg aatttatccc gcacctggca ggaagtaaaa
    18601 ggcagcgacg atgcctggtt ttacaccgtt tttcagctta gtggtcaggc aataatggag
    18661 caggatgagc gtcaggtgca gattggcgct ggcgatatta cgttactcga tgcctcacgc
    18721 ccctgttcgc tttactggca ggagtcttct aaacagattt cattactttt gccacgcact
    18781 ctgctggaac aatattttcc ccatcaaaaa cctgtctgcg cagaaagact ggacgctgac
    18841 ttacccatgg tgcaactcag tcatcgcctg ttacaggaga gcatgaataa tccggcactt
    18901 tctgaaacag aaagtgaagc tgcgctacag gcgatggtgt gtctgctgcg cccggtactt
    18961 catcagcggg aatctgttca acctcgtcgt gaacgtcagt ttcaaaaagt ggttacgttg
    19021 atagacgata atattcgcga agagatatta cgcccggagt ggatagccgg agagacaggt
    19081 atgtcagtac gtagtttgta ccgaatgttt gccgataaag gtttggtagt cgcgcaatat
    19141 attcgtaacc gtcgtctcga tttttgtgca gatgcgattc gccatgccgc agatgatgaa
    19201 aaactggcag gcatcggctt tcattgggga ttttctgacc agagtcattt ttcaacggta
    19261 tttaagcaac gctttgggat gacgccaggc gagtatcgac gtaaattccg ctaactaatt
    19321 ggcgttgtgt gtactgcaac gccaattagt taaaacagat atttgactat gcatttttca
    19381 gggcgaaagg caatttgaca ggagtttttg aatattcacc ttgtttcagg aaaatagatc
    19441 aatcgctttc tcctgtagcg ttatcccgat attgcggttt tgctattaaa attttcgcac
    19501 ccatttaccg tttatccctt gtacatattc ccccggtttg gcccgagcta cgagcttttg
    19561 cccggcgagt ttcgcgatat catctacaga cacattattt tgctttgcca gttgttggta
    19621 actcgcttta cgttcggcat taatgtcggt tactaatttc tctgtttcag catccgtttt
    19681 cagcgcaacc agataaccat aaaatgtttc acctacccgc ccctgagttc tggcttcatc
    19741 cagagtcaat gccattacat tgctgcttac cagcccaaca agaaacgcac aaagaagtaa
    19801 tgttttcttc atcatcgcct cagaaaagat cgctacgagt ttcaagcagc tcttcgacat
    19861 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
    19921 gttccttggg cgcagcgact tcaatacgcg gcgtacaggc agccagcata aaagatgacg
    19981 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
    20041 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
    20101 actgcgccac agcgtaaaaa tattctcttc atgatagtaa ttaaggttta ccgtaccgct
    20161 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
    20221 atctaagtta atttttgtcg atgaccggct aatttccata tagcgcaacc agttaattgc
    20281 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
    20341 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
    20401 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
    20461 gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg
    20521 catacgtaat tgtttcatca gtacgttacc gcctaacaca tcgacgctaa catcagtcaa
    20581 cagcaagggt tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt
    20641 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
    20701 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
    20761 atctggcatc caggcactac cgcctttcaa cacgccgtgt cctcccgcgc ggaatccttg
    20821 ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt
    20881 ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgttttggcc accaggcgtt
    20941 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
    21001 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa
    21061 ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
    21121 cgcgccgctg aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
    21181 taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag
    21241 gtcggtcagc gtaatcgtgc tgtcatgcca ttcacctttt cctgcgacat cccaggtggc
    21301 attcatcggt gtaaaactgc cttttcccca gtagcgccac tgccagcggc ccgcgtcagg
    21361 gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg
    21421 cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc
    21481 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag
    21541 taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg
    21601 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
    21661 ctgactgtta tccatgctta attttcctgg gccaaaattt agcaccgcgt tgcctttacc
    21721 cgcttgcccc tgggtcagca cactcagtcg tccgctgatc agagcgttct caagccctgc
    21781 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
    21841 actccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
    21901 caacggatcg ccgttatccc gtgccagcac aatcaattgc ccgctatttt cctgccagtc
    21961 cagctctgca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
    22021 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
    22081 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
    22141 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
    22201 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
    22261 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
    22321 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctacca tgccgacgtt
    22381 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
    22441 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
    22501 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
    22561 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
    22621 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
    22681 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
    22741 agttgttgat gaatttttca atatcgccat agctttcaat ta
//
LOCUS       DAFAVF010000061        22672 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFAVF010000061 DAFAVF010000000
VERSION     DAFAVF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.061"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(63..383)
                     /gene="chbB"
                     /locus_tag="LGK42_002351"
     CDS             complement(63..383)
                     /gene="chbB"
                     /locus_tag="LGK42_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBK2658594.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(682..1020)
                     /gene="osmE"
                     /locus_tag="LGK42_002352"
     CDS             complement(682..1020)
                     /gene="osmE"
                     /locus_tag="LGK42_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBK2658595.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1222..2049
                     /gene="nadE"
                     /locus_tag="LGK42_002353"
     CDS             1222..2049
                     /gene="nadE"
                     /locus_tag="LGK42_002353"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK2658596.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2279..3166
                     /gene="cho"
                     /locus_tag="LGK42_002354"
     CDS             2279..3166
                     /gene="cho"
                     /locus_tag="LGK42_002354"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBK2658597.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3126..3701)
                     /gene="ves"
                     /locus_tag="LGK42_002355"
     CDS             complement(3126..3701)
                     /gene="ves"
                     /locus_tag="LGK42_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBK2658598.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3904..4389)
                     /gene="spy"
                     /locus_tag="LGK42_002356"
     CDS             complement(3904..4389)
                     /gene="spy"
                     /locus_tag="LGK42_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBK2658599.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4719..5687)
                     /gene="astE"
                     /locus_tag="LGK42_002357"
     CDS             complement(4719..5687)
                     /gene="astE"
                     /locus_tag="LGK42_002357"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBK2658600.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5680..7023)
                     /gene="astB"
                     /locus_tag="LGK42_002358"
     CDS             complement(5680..7023)
                     /gene="astB"
                     /locus_tag="LGK42_002358"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBK2658601.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7020..8498)
                     /gene="astD"
                     /locus_tag="LGK42_002359"
     CDS             complement(7020..8498)
                     /gene="astD"
                     /locus_tag="LGK42_002359"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBK2658602.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8495..9529)
                     /gene="astA"
                     /locus_tag="LGK42_002360"
     CDS             complement(8495..9529)
                     /gene="astA"
                     /locus_tag="LGK42_002360"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBK2658603.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9526..10746)
                     /gene="astC"
                     /locus_tag="LGK42_002361"
     CDS             complement(9526..10746)
                     /gene="astC"
                     /locus_tag="LGK42_002361"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBK2658604.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11192..11998
                     /gene="xthA"
                     /locus_tag="LGK42_002362"
     CDS             11192..11998
                     /gene="xthA"
                     /locus_tag="LGK42_002362"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK2658605.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12165..12875
                     /locus_tag="LGK42_002363"
     CDS             12165..12875
                     /locus_tag="LGK42_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK2658606.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12880..13557
                     /gene="ydjY"
                     /locus_tag="LGK42_002364"
     CDS             12880..13557
                     /gene="ydjY"
                     /locus_tag="LGK42_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBK2658607.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13572..14279
                     /locus_tag="LGK42_002365"
     CDS             13572..14279
                     /locus_tag="LGK42_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK2658608.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14279..14827
                     /locus_tag="LGK42_002366"
     CDS             14279..14827
                     /locus_tag="LGK42_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK2658609.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14837..16003
                     /locus_tag="LGK42_002367"
     CDS             14837..16003
                     /locus_tag="LGK42_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2658610.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15976..17511
                     /locus_tag="LGK42_002368"
     CDS             15976..17511
                     /locus_tag="LGK42_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK2658611.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17511..18164
                     /locus_tag="LGK42_002369"
     CDS             17511..18164
                     /locus_tag="LGK42_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK2658612.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDSFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18231..19538
                     /gene="ynjE"
                     /locus_tag="LGK42_002370"
     CDS             18231..19538
                     /gene="ynjE"
                     /locus_tag="LGK42_002370"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBK2658613.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19547..20167)
                     /locus_tag="LGK42_002371"
     CDS             complement(19547..20167)
                     /locus_tag="LGK42_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK2658614.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20254..20661
                     /gene="nudG"
                     /locus_tag="LGK42_002372"
     CDS             20254..20661
                     /gene="nudG"
                     /locus_tag="LGK42_002372"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBK2658615.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20627..20899)
                     /locus_tag="LGK42_002373"
     CDS             complement(20627..20899)
                     /locus_tag="LGK42_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBK2658616.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21135..22478
                     /gene="gdhA"
                     /locus_tag="LGK42_002374"
     CDS             21135..22478
                     /gene="gdhA"
                     /locus_tag="LGK42_002374"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK2658617.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         5388 a         6030 c         5813 g         5441 t
ORIGIN      
        1 caatttaaat attgcggtat tgatttatga aataactctt tgacgggaaa atttaaaata
       61 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
      121 tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc
      181 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
      241 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
      301 tctgcctgtg cgcgcatttt tgataccagt aaagaggtag acatgcccgc agaacaaaac
      361 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
      421 actccgcaag ccttaacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
      481 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttgt
      541 aaattgtctc tccttgacct gcggttatga ccctcttcac atttcgggca aataattcgc
      601 gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgttac
      661 tcaagctttc agacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      721 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      781 tgctttacca tcacgttgac ccagaatgta ggtctggcaa gtaccacgag catggatcat
      841 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      901 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      961 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
     1021 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1081 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca
     1141 ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc
     1201 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1261 aaaccgcaga ttaatgccga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1321 ctgcgaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1381 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga aaccggcaac
     1441 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1501 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1561 gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc
     1621 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1681 agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa
     1741 tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa
     1801 cagttgctga cggcattagg ttgcccggaa cacctttata agaaagcccc aacggccgat
     1861 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1921 atcgacgact atctggaagg gaaaaacgta cctgaacagg tcgccagaac aatagagaac
     1981 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     2041 aaaaagtaat aatttgcaca taatggtttg tgcgaaaaag agtaatttgt tcacgccgga
     2101 tgcggcgtga acgccttatt cgacctacaa aactatgcaa attcaatata ttgcaggagt
     2161 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2221 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2281 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2341 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2401 tggcgaaagt gacaccatgc cgctctatat cggcaaaagc attaacatcc gcagccgcgt
     2461 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacgcatcag
     2521 ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2581 acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt
     2641 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2701 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga
     2761 tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg gtcgtgcatg
     2821 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2881 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2941 aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     3001 ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc
     3061 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3121 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3181 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3241 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3301 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3361 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gccctgccgt cagtttcgct
     3421 tttaccacct ggtccgccgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3481 cggtctgcgc tttcaaggaa catctcgccg ccttccagca acgtcactat cctttccatg
     3541 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3601 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcgtttcgc
     3661 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3721 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3781 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3841 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3901 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3961 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     4021 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     4081 cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg
     4141 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4201 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4261 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc
     4321 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
     4381 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
     4441 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
     4501 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
     4561 agagtgatga gagaataata caacacgatg attttgcaga gattatgaag aactataccg
     4621 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
     4681 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
     4741 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
     4801 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
     4861 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
     4921 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
     4981 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
     5041 ggcaacgctt tgccaagttc cagcgtacag gccagcgcgc caaaatgtct ggcgctgaaa
     5101 tgggtaaacg taccaccagg ttcctgatgg aacaccagcg cctccagccc cgccgcaccc
     5161 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc
     5221 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccagcgcac agattctttg
     5281 cccaggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     5341 ctttcagcaa atagctgcca acgaccgcca aacattcgat tcatatcgct atggcaataa
     5401 cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac
     5461 gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatctccac aggtgccgtc
     5521 tcattaccgt gtattcccgc tgaaatcacc agtgcgcctt gcggtggcgt taatggcgtc
     5581 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     5641 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     5701 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     5761 ccgccctccg cgcagcaatt gcgggtcggc cagatcggca gcagtaaggc gatcgcggta
     5821 gtaacgatcc acccagtcat tgagcgcatt aaacagcgta tcgttcatca tcaccgccgg
     5881 attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc
     5941 gccgccattc gccatgcttt cacgtaaatc aaagactttt agttcgctaa tcgggttgtc
     6001 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     6061 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     6121 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     6181 cacccgcgca cgcaggtttg ccagtaactg tgactggcga gcgaacgcct gttggtggca
     6241 aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc cctggtcgat
     6301 aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat tcagccttgc
     6361 caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt
     6421 gccttcttct cgcccgtaga caaaaagttg catacccggc tcaccgtaat gaccgccgag
     6481 acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg
     6541 aacgctaaat ttctcttcgt cgttaaaaat cgcttttaaa agtgattcgg tgacgtgcgc
     6601 ttccagcgag cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc
     6661 cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga
     6721 actgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc
     6781 gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcgggat
     6841 cacggcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc
     6901 cgcctgtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga
     6961 caggccagcg taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt
     7021 catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgttgcggg caaagttaac
     7081 gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg
     7141 gggcgatggt taccggatgc accaatgccg ccgaatggcg cggtactggc agcaccagta
     7201 agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt
     7261 tcccgctcgg gggaaaccag accgcaagag agtccgaagc gagtgttatt cgccattaga
     7321 atcgcttcct cgaaagaatc ataacgccag acgcgcaata acggtccgaa cacctcttca
     7381 tctggtacgc cagcaacgcc tgtcatttca atgatccccg gcgtcagcaa cgatgtctct
     7441 gattgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag
     7501 gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt
     7561 tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagacg agcaagaaac
     7621 gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg
     7681 gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca
     7741 tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg
     7801 gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg
     7861 agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagt
     7921 acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt
     7981 gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt
     8041 ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg
     8101 cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg
     8161 tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct
     8221 tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcgcc tttattcctt
     8281 tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc
     8341 cagcgcggaa acgccgcacg ggctgcgcga caagcctgcc cgacctgagc ggcatcggca
     8401 tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc
     8461 tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca
     8521 ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg
     8581 ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat
     8641 aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca
     8701 cttccaccag ccgactttta cgaatggcgc gcacgcggtc gatgtcacac tcaagcgttg
     8761 gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg
     8821 cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata
     8881 aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg
     8941 tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt
     9001 gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt
     9061 cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc
     9121 cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg
     9181 tgtgatcgtt actgagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg
     9241 cgtgaaccaa cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct
     9301 caatggcaca aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc
     9361 cctgctcact tttgggcagt tcgccttgcc aggttttgat tgccctttcg atacgcgccg
     9421 aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct
     9481 gcatcagcgc cgagacatct gatcgctcaa cgggacggat gaccatcatg atgaacctcg
     9541 gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc
     9601 gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac
     9661 gcctgctttc gccgcttcct gagagatctg tttcgcttgc ccggcgtaat cggcattcag
     9721 tacacagcca atcagcaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt
     9781 aatagtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc
     9841 tggtgtgttg atgagctcca gcactttgcc tgccaccgcc gaggccagcg gattaccgcc
     9901 ataggtggtg ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa
     9961 cgcaccgacc gggaaaccgc cgcccagcgc tttggcggta gttaacagat caggcgtcac
    10021 gccgtagtgc atataggcat acagttcccc ggtgcgcccg acgccggttt gtacttcatc
    10081 aaaaatcaac agcgcattgt ggcggttaca caattcacgc agaccttgta aaaacgcgtt
    10141 gctggctggc accacaccgc cttccccctg gatgggttcg acaatcaccg cacaggtaga
    10201 gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc
    10261 cggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag
    10321 cgtgcgacca tgaaacgcat ttttgaacgc cacgatgccg ctcttatggc tgccgtagcg
    10381 gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt
    10441 acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac
    10501 cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc
    10561 ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc
    10621 gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa
    10681 gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg
    10741 agacatagcg acctctacag taaataattc gttatttata tgttaacaat aagtaatgtt
    10801 tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc
    10861 ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa
    10921 agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa
    10981 gtaaaatata attctggaat tgtgatcatt gacgaaattt actggaaatt actgcgccat
    11041 tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa
    11101 ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac
    11161 gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg
    11221 cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat
    11281 tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct
    11341 cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa
    11401 agagacgccg attgccgtgc gtcgcggttt tcccggtgac gacgaagaag cgcagcggcg
    11461 aattattatg gcggaaatcc cctcgccgct gggcaatgtc accgtgatca acggttactt
    11521 cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaggcgc agttttatca
    11581 gaatctgcaa aactatctgg aaaccgaact caaacgtgat aatccggtac tgattatggg
    11641 cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg
    11701 ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct
    11761 gatgagctgg gggttggtcg ataccttccg ccatgcgaat ccacaaacag cagatcgttt
    11821 ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct
    11881 gctgctcgcc agccaaccgc tggcagaatg ttgtgtagaa accggcatcg actatgaaat
    11941 tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt
    12001 tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac
    12061 tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt
    12121 ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta
    12181 aatttctttt ggcctgtctt atttttgcgc tggtcattta cgctatccac actttcggtt
    12241 tattcgatct gctcaccgat ttaccccact tacagacact catccgccag agcggattgt
    12301 tcggttatag cctctatatt ctgttattca ttattgccac tctctttctg ttaccaggaa
    12361 gcatactggt gatcgctgga ggaatagttt ttggcccgct cttagggaca ctactctcat
    12421 taattgccgc cacgctcgcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg
    12481 atttactgct gaaatacgtt ggtcatagcc atacctttca ggccattgaa aaaggcattg
    12541 cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata
    12601 ttcaaaatta cgcttacgga ttaaccacaa tcgccttctg gccttatacc cttatttcgg
    12661 cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg
    12721 aaggcattac gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc
    12781 tcgtccagct cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc
    12841 gcagcccact tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg
    12901 tcatggaaaa aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc
    12961 gatcaaaaag agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg
    13021 ctgcgcgtcg atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc
    13081 ctcaccgacg acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg
    13141 ctgtttatgg cttatgcgac cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt
    13201 acgccgggcg aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa
    13261 ctggatattt cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt
    13321 gttgacagta atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa
    13381 gagaagaaaa cgggttgcct ggtgtgtctg gatagctgcc cagtcggcat cgtcagcaat
    13441 gcaacataca cttatggtgc ggtggaaaaa cgcggcgaag tgaaattcaa aggcaatgcc
    13501 tcagttctcc cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc
    13561 caggataaag gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca
    13621 tcatcctgtt gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta
    13681 tcaatcgcag cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc
    13741 agtcttacgg cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg
    13801 ctgcaccgct acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct
    13861 ggggcggctt gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg
    13921 ccagagtgat gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta
    13981 tggacggctt tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt
    14041 ttgtcccttt cgatccaatc agctatgctg ccggtctgac ttcaatacgt tttcgctcgt
    14101 tttttatcgc caccgggctt ggtcaattac cggcgactat tgtttattcc tgggcgggca
    14161 gcatgttaac aggcggtact ttctggtttg tcaccggact gtttattctg tttgccctga
    14221 ccgtggtgat ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgcctgat
    14281 gggtttaccg ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg
    14341 tcgccattac ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt
    14401 ttatttggtg tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt
    14461 agtgcgatcg cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga
    14521 tatcaccagt atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc
    14581 tgactggcgg caaagcccgc tcttcagcga tgaagaacgg ctggcgctgg agtacgccga
    14641 agccgcaagc gtaacgccgc caacggtcga tgatgccctg cgcacccgac tggctgcgca
    14701 ttttgacgct caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc
    14761 tcgttttaat tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg
    14821 ttcttaagga gagaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc
    14881 tctggcaaca catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt
    14941 ctggtttaac gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg
    15001 cgagatgaaa acccattacg ctataaacct gaagattgtt cgtctggcgg atgccgcaga
    15061 cgcggtgaag cgcattcaga ccgaagctgc tgccggacgt aaaacgggcg gctcggtgga
    15121 tctgctctgg gtgaacggcg aaaacttccg caccttaaaa gaggccaatt tattacaaac
    15181 gggctgggcg gagactctgc ccaactggcg ctatgtcgac acacagctgc cggtgcggga
    15241 agatttttca gtgcctacag aaggggctga atcgccctgg ggcggcgcac aactgacgtt
    15301 tatcgcccgc cgcgatgtta cgccacagcc accacaaacg ccgcaagcct tactggagtt
    15361 tgctaaagcc aatcccggca cggttaccta tccgcgccca ccggacttta ccggcacggc
    15421 gtttcttgaa cagttgctga ttatgctgac gcccgatccc gccgcgttaa aagttgcccc
    15481 ggataaagcc actttcgccc gcgtcactgc gcccttgtgg caatatcttg acgcgctaca
    15541 tccctattta tggcgcaaag gaaaggattt cccgccttcg cccgcgcgga tggatgctct
    15601 gctgaaagcc ggcacattgc gcctgtcgct gacctttaac cctgctcatg cccagcaaaa
    15661 aatcgccagc ggcgatttgc ctgcaagcag ttacagtttt ggttttaacc aggggatgat
    15721 tggcaacgtg catttcgtca ccattcctgc taacgcaaat gccagtgctg cggcgaaggt
    15781 ggttgccaac ttcctgctct cacccaatgc gcaactgcgt aaagcggatc ccgctgtctg
    15841 gggcgatcct tctgttctcg atccgcaaaa actgcctgat gggcagcgcg aaacattgca
    15901 atcaagaatg ccgcaagatc tgccgccggt actggctgaa ccgcacgcag gttgggtaaa
    15961 tgcgctggaa caagaatggc tacaccgtta cggtacgcat taatcttttt gctgtgggcg
    16021 atggtggcgg tgatttatgc accactgatc ccggcagctc tcacgctgat atcgcctgct
    16081 ttgtcgttga cacactggca ggcgttattt gccgatccac agttaccgca ggcattactg
    16141 gcaacgctgg tgtcgacaac catcgcggcg gtcggggcgt tgttgattgc cctgctggtg
    16201 attgtggcgc tgtggccttg gccgaaatgg cagcgtatgt gtgcccgtct gccgtggctg
    16261 ctcgccattc cccatgtggc ttttgccacc agcgcccttc tgctctttgc tgagggaggg
    16321 ctgctttatg actatttccc gtatttcact ccgctaatgg acaaattggg tattgggctg
    16381 ggcctcaccc ttgcagtgaa agaaagcgca tttctgctgt ggatcttagc ggcagtattg
    16441 agcgaaaaac ggctgttgca gcaggtcatt gtgctggatt cgctgggcta cagccgctgg
    16501 caatgcctga actggctgct cttgccctcc gtcgcacctg cactggcaat ggcgatgttg
    16561 gcgattgttg cctggtcgct ttcggtcgtg gatgtggcga ttattcttgg gccaggtaat
    16621 ccaccgacgc tggcagtaat tagctggcag tggttaactc agggcgacgc ggatcaacaa
    16681 acgaaaggcg cactcgccag tctgctgctg atgctgttac tcgccgccta cgttttgctg
    16741 agctatctgc tatggcgcag ctggcggcgc actattcccc gcgtagatgg cattcgcaag
    16801 cctgccacac ctttattacc aggcactacg ctggcgagtt ttttaccctt aaccggtgtg
    16861 ctgtgtgtgg ttctgctggc gatcctcgcg gatcagtcga cgatcaatag tgaagcactc
    16921 atcaacagcc tgacaatggg gctggtggcg acattcatcg ctttgctcct gttactgctg
    16981 tggctggaat gggggccaca gcgtcgccag ttgtggctct ggttgcccat tctattgccc
    17041 gctctgccac tggtggcggg ccagtacacg ctggcgctat ggctgaacct ggatggaagc
    17101 tggacggcgg tggtctgggg gcatctgctg tgggtaatgc cgtggatgct gtttatcctg
    17161 caacccgcct ggcagcgcat tgattcacgg ttaattttga ttgcgcaaac actgagctgg
    17221 tcgcgggcca aaatcttctt ttacgtgaaa tgtccgctta tgctacgccc tacgctgatt
    17281 gccttcgcgg tgggattttc agtcagtatt gcgcagtata tgccgacgct gtggctgggc
    17341 gcggggcgtt ttccgacgct caccactgag gcggtggcat taagcagcgg cggcagcaat
    17401 ggtattttcg ccgcccaggc tttatggcaa ctgctattac cgcttattat ttttgccctg
    17461 accgcgttag tcgcaaaatg ggtaggttat gtcagacaag gactccgcta atgctctgcg
    17521 tgaaaaatgt ttcgctacgt ttaccagaaa gccgcttgct gacaaacgtt aactttacgg
    17581 tggataaagg tgacattgtc acgttaatgg ggccgtctgg ctgtggaaaa tccactctgt
    17641 tttcatggat gattggtgca ctggctggac agttttcttg tacaggtgag ctatggctca
    17701 atgagcaacg gattgacatg ctacccaccg cacagcgtca gattggcatt cttttccagg
    17761 atgcactgtt atttgaccag ttcagcgtcg ggcaaaattt actgctggcg ctaccgtcga
    17821 cgctcaaggg gactgccagg cgcaatgccg tgaaagacgc gcttgatcga gcgggactgg
    17881 ctgaaactta tcaccaggac cctgcgacat tatcaggcgg tcagcgagcg cgggtggccc
    17941 tgctgcgcgc ccttctcgct cagccaaaag cgttactcct ggatgagcca ttcagccgtc
    18001 ttgatgtggc tctgcgcgat agttttcgcc agtgggtgtt cagcgaagtt cgcgaactgg
    18061 cgatcccggt cgttcaggta acgcacgatc tccaggatgt tcctgctgat agttctgttc
    18121 tggatatggc gcagtggtca gaaaattaca acaaactgcg ataacgcaaa gtttttctca
    18181 atgcgtcagt tcagaatggc gcactcaaaa ctacaatgtc gggattttcg atgaaacgtg
    18241 tttctcaaat gaccgcgctg gcaatggctt tagggctggc ttgcgcttct tcgtgggccg
    18301 ctgaactggc gaagcctctt acacttgacc agcttcaaca acaaaatggc aaagcgatag
    18361 atactcgccc cagcgcgttt tataacggct ggccacaaac cttaaatggc ccttctggtc
    18421 atgaacctgc cgccttaaac ctctctgcca gctggcttga caaaatgagc accgaacagc
    18481 tcaacgcgtg gatcaagcaa cataacctga aagccgatgc cccggtagcg ctgtacggta
    18541 atggcaaaga tgtcgacgcc gtcaaaacgc gactgcaaaa agcaggtttt acgcatatct
    18601 ccattctgag tgacgcgcta agcgaacctt cccgtctaca aaaactgccg cactttgagc
    18661 agctggttta tccgcaatgg cttcacgacc tgcaacaagg taaagaagtt acggcgaaac
    18721 ctgccggtga ctggaaagtc attgaagcgg cctggggcgc tccaaaattt taccttatca
    18781 gccatattcc cggcgctgac tacatcgata ccaacgaagt ggaaagcgaa ccactgtgga
    18841 acaaagtttc tgatgaacaa ctaaaagcga tgctggcaaa acacggcatt cgccatgaca
    18901 ccacggtcat tctgtacggg cgtgacgtat acgctgcagc gcgtgtggca caaattatgc
    18961 tctacgctgg cgtgaaagat gtgcgcctgc tggacggcgg ctggcaaacc tggtccgacg
    19021 cgggtctgcc cgttgagcgc ggtacgccac cgaaagtgaa agcggaaccg gatttcggcg
    19081 tgaagatccc ggcgcaaccg cagttgatgc tcgatatgga acaggcccgt ggactgctgc
    19141 atcgccagga tgcatcgctg gtgagcattc gttcgtggcc agaatttatc ggtacgacca
    19201 gcggttacag ctatattaaa ccaaaaggtg aaatagccgg agcacgttgg ggacacgctg
    19261 gtagcgactc gacgcatatg gaagatttcc ataacccgga tggcaccatg cgtagcgccg
    19321 atgatattac cgctatgtgg aaagcatgga atatcaaacc agatcagcaa gtttcattct
    19381 actgcggcac cggctggcgc gcgtccgaaa cctttatgta cgcacgcgcc atgggttgga
    19441 agaatgtttc cgtgtatgac ggcggctggt acgaatggag cagcgatcca aaaaatccgg
    19501 tagcaaccgg tgaacgcggc ccggacagta gcaaataaca ttgcaattac tgacgctggt
    19561 gcgacttcag cgtcagatac ccactccaca cccgcgtaaa ggtcgtcatc cagcacaacg
    19621 caccgaagat ccacgcaaac cacgcaaacc atgcagggaa taaacatccc agcacaaaca
    19681 gtaagatcgt ttctgtgcct tcggttaacc cgcccagata ataaaacgat ttatgcgcat
    19741 aaccgggatt atcaatctga tgtttcgccg ccagcgcagc aaaagcgaga aaactgctgc
    19801 ctgtgccgat aaacgcaaac agcagccagc cgcctgccag ggcgttttgc tgcggtgcgg
    19861 cgaggataaa cccaaaaggt accagcgcgt aaaaaagaaa atcgagagaa atatcaagaa
    19921 agccgcccgc gtcggtaagc cctctgcgcc gcgccagcgc accgtccaga ccatcaagca
    19981 gcctgttcaa caaaatgacg acaagcgccg ccagatacca gcccagcgcc agaaaaggca
    20041 gtgccagcac gccgatggca aatcccacta atgttaaacc atctggcgta atgcccggtt
    20101 tatcaagaac ccgcacgcac tgatgcaaca acggtttaat ccggggatga agatggcggt
    20161 ctagcacagg cactccttaa atataaagcc tttctgattg agcaacagtg cggatattat
    20221 ggcatttttc gcttatctgc ccgtgtgtaa tttatgaaaa tgattgaagt tgttgccgcc
    20281 atcattgaac gtgatggcaa aattttactc gcgcaacgcc ccgcccagag cgatcaggcg
    20341 ggattatggg agtttgccgg tggtaaagtc gagccggatg aaagccagcg gcaggcgctg
    20401 gtgcgtgagt tacgcgaaga actgggcatc gaagcaactg tgggtgagta tgttaccagc
    20461 catcagcgag aagtttcggg ccggattatc catcttcatg cctggcacgt acccgacttc
    20521 cacgggacgt tacaggcaca tgaacatcag gcgctggtct ggtgctcacc tgaagaggcg
    20581 ctgcaatatc cgctggcccc tgctgacatt ccattattag aggcgtttat ggctttacgc
    20641 gccgccagac cagcggatta gtgctaaggg ttttgtcatc ccgctggcat tgcagcagta
    20701 ttccctcggc tttaattacc gcgccttcag aataattttt atcctgataa acgcagcact
    20761 gagtacaggg ctgcgctgac tgcccgcctg aactgaacac ttctggcggt acgtttacct
    20821 ccacgtccgg acgataatgc gggttagcca gtgcaattaa tggaaatgct aatactacgg
    20881 cgaacaatgc tcgactcaca gggaactcct taacgttatt gtctctgcta ctgataacgg
    20941 tagccgggtg gcaaaacttt agcgtctgag ttatcgcatt tggttatgag attactctcg
    21001 ttattaattt gctttcctgg gtcatttttt tcttgcttac cgtcacattc ttgatggtat
    21061 agtcgaaaac tgcaaaagca catcacataa acaacataag cacaatcgta ttaatatata
    21121 agggttttat atctatggat cagacatatt ctctggagtc attcctcaac catgtccaaa
    21181 agcgcgaccc gaatcaaacc gagttcgcgc aagccgttcg tgaagtaatg accacactct
    21241 ggccttttct tgaacaaaat ccaaaatatc gccagatgtc attactggag cgtctggttg
    21301 aaccggagcg cgtgatccag tttcgcgtgg tatgggttga tgatcgcaat caggtacagg
    21361 tcaaccgtgc atggcgtgtg cagttcagct cagccatcgg cccgtacaaa ggcggtatgc
    21421 gcttccatcc gtcagttaac ctctccattc tcaaattcct cggcttcgaa caaaccttca
    21481 aaaatgccct gactaccctg ccgatgggcg gtggtaaagg cggtagcgat tttgacccga
    21541 aaggtaaaag cgaaggcgaa gtgatgcgtt tctgccaggc gctgatgacc gagctttatc
    21601 gtcatctggg cgcggatacc gacgttccgg caggtgatat cggcgttggt ggtcgtgaag
    21661 tcggctttat ggcggggatg atgaaaaagc tctccaacaa taccgcctgc gtcttcaccg
    21721 gtaagggcct ttcatttggc ggtagtctta ttcgcccgga agctaccggc tacggtctgg
    21781 tttatttcac agaagcaatg ttaaaacgcc acggtatggg ttttgaaggg atgcgcgttt
    21841 ccgtttctgg ctccggcaac gtcgcccagt acgctatcga aaaagcgatg gaatttggtg
    21901 ctcgtgtgat cactgcgtca gactccagcg gcactgtagt tgatgaaagc ggattcacga
    21961 aagagaaact ggcacgtctt atcgaaatca aagccagccg cgatggtcga gtggcagatt
    22021 acgccaaaga atttggtctg gtctatctcg aaggccaaca gccgtggtct gtaccggttg
    22081 atatcgccct gccttgcgcc acccagaacg aactggatgt tgacgccgcg catcagctta
    22141 tcgccaatgg cgttaaagcc gtcgccgaag gggcaaatat gccgaccacc atcgaagcga
    22201 ctgaactgtt ccagcaggca ggcgtactgt ttgcaccggg taaagcggct aatgctggcg
    22261 gtgtagcaac gtcaggcctt gaaatggcgc aaaacgctgc gcgcctgggc tggaaagccg
    22321 agaaagttga cgcacgtttg catcacatca tgctggatat ccaccatgcc tgtgttgagc
    22381 atggtggtga aggtgagcaa accaactacg tgcagggcgc gaacattgcc ggttttgtga
    22441 aggttgccga tgcgatgctg gcgcagggtg tgatttaagt tgtaaatgcc tgatggcgct
    22501 acgcttatca ggcctacaaa tgggcacaat tcattgcagt tacgctctaa tgcaggccgg
    22561 gcaagcgcag cgcccccggc aaaatttcag gcgtttatga gtatttaacg gatgatgctc
    22621 cccagggaac atttcttatg ggccaacggc atttcttact gtagtgctcc ca
//
LOCUS       DAFAVF010000062        22160 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFAVF010000062 DAFAVF010000000
VERSION     DAFAVF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.062"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            8..505
                     /gene="ftnA"
                     /locus_tag="LGK42_002375"
     CDS             8..505
                     /gene="ftnA"
                     /locus_tag="LGK42_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBK2658618.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(543..782)
                     /locus_tag="LGK42_002376"
     CDS             complement(543..782)
                     /locus_tag="LGK42_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBK2658619.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            973..2184
                     /gene="tyrP"
                     /locus_tag="LGK42_002377"
     CDS             973..2184
                     /gene="tyrP"
                     /locus_tag="LGK42_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBK2658620.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2246..2911)
                     /gene="yecA"
                     /locus_tag="LGK42_002378"
     CDS             complement(2246..2911)
                     /gene="yecA"
                     /locus_tag="LGK42_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBK2658621.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3107..3193)
                     /locus_tag="LGK42_002379"
     tRNA            complement(3107..3193)
                     /locus_tag="LGK42_002379"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3157..3159),aa:Leu,seq:taa)
     gene            complement(3206..3279)
                     /locus_tag="LGK42_002380"
     tRNA            complement(3206..3279)
                     /locus_tag="LGK42_002380"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3245..3247),aa:Cys,seq:gca)
     gene            complement(3334..3409)
                     /locus_tag="LGK42_002381"
     tRNA            complement(3334..3409)
                     /locus_tag="LGK42_002381"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3374..3376),aa:Gly,seq:gcc)
     gene            complement(3561..4109)
                     /gene="pgsA"
                     /locus_tag="LGK42_002382"
     CDS             complement(3561..4109)
                     /gene="pgsA"
                     /locus_tag="LGK42_002382"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK2658622.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4166..5998)
                     /gene="uvrC"
                     /locus_tag="LGK42_002383"
     CDS             complement(4166..5998)
                     /gene="uvrC"
                     /locus_tag="LGK42_002383"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK2658623.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5995..6651)
                     /gene="uvrY"
                     /locus_tag="LGK42_002384"
     CDS             complement(5995..6651)
                     /gene="uvrY"
                     /locus_tag="LGK42_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBK2658624.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6947..7123
                     /locus_tag="LGK42_002385"
     CDS             6947..7123
                     /locus_tag="LGK42_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658625.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7110..7334
                     /locus_tag="LGK42_002386"
     CDS             7110..7334
                     /locus_tag="LGK42_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBK2658626.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7401..8123)
                     /gene="sdiA"
                     /locus_tag="LGK42_002387"
     CDS             complement(7401..8123)
                     /gene="sdiA"
                     /locus_tag="LGK42_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBK2658627.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8353..9105)
                     /gene="yecC"
                     /locus_tag="LGK42_002388"
     CDS             complement(8353..9105)
                     /gene="yecC"
                     /locus_tag="LGK42_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBK2658628.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9102..9770)
                     /gene="tcyL"
                     /locus_tag="LGK42_002389"
     CDS             complement(9102..9770)
                     /gene="tcyL"
                     /locus_tag="LGK42_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBK2658629.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9785..10771)
                     /gene="dcyD"
                     /locus_tag="LGK42_002390"
     CDS             complement(9785..10771)
                     /gene="dcyD"
                     /locus_tag="LGK42_002390"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBK2658630.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10876..11676)
                     /gene="tcyJ"
                     /locus_tag="LGK42_002391"
     CDS             complement(10876..11676)
                     /gene="tcyJ"
                     /locus_tag="LGK42_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2658631.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11764..12315)
                     /gene="fliZ"
                     /locus_tag="LGK42_002392"
     CDS             complement(11764..12315)
                     /gene="fliZ"
                     /locus_tag="LGK42_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBK2658632.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPSTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12361..13080)
                     /gene="fliA"
                     /locus_tag="LGK42_002393"
     CDS             complement(12361..13080)
                     /gene="fliA"
                     /locus_tag="LGK42_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBK2658633.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13400..15112)
                     /locus_tag="LGK42_002394"
     CDS             complement(13400..15112)
                     /locus_tag="LGK42_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBK2658634.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVNAKITFGSGMTVDFTQASKKVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEVTTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            15378..16784
                     /gene="fliD"
                     /locus_tag="LGK42_002395"
     CDS             15378..16784
                     /gene="fliD"
                     /locus_tag="LGK42_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBK2658635.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16809..17219
                     /gene="fliS"
                     /locus_tag="LGK42_002396"
     CDS             16809..17219
                     /gene="fliS"
                     /locus_tag="LGK42_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBK2658636.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            17219..17584
                     /gene="fliT"
                     /locus_tag="LGK42_002397"
     CDS             17219..17584
                     /gene="fliT"
                     /locus_tag="LGK42_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBK2658637.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17662..19149
                     /gene="amyA"
                     /locus_tag="LGK42_002398"
     CDS             17662..19149
                     /gene="amyA"
                     /locus_tag="LGK42_002398"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBK2658638.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQERVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(19183..19596)
                     /locus_tag="LGK42_002399"
     CDS             complement(19183..19596)
                     /locus_tag="LGK42_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2658639.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19783..20988
                     /gene="yedE"
                     /locus_tag="LGK42_002400"
     CDS             19783..20988
                     /gene="yedE"
                     /locus_tag="LGK42_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBK2658640.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMVVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            20985..21218
                     /gene="yedF"
                     /locus_tag="LGK42_002401"
     CDS             20985..21218
                     /gene="yedF"
                     /locus_tag="LGK42_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBK2658641.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            21325..21996
                     /locus_tag="LGK42_002402"
     CDS             21325..21996
                     /locus_tag="LGK42_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBK2658642.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEISGKEASEIAVSGCVPAKQFSWHPVLRAVGNVKNQGAAL
                     IQPVC"
BASE COUNT         5415 a         5698 c         5410 g         5637 t
ORIGIN      
        1 cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga acctggaact
       61 gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata ccttcgaagg
      121 tgctgccgcg ttcctgcgcc gtcacgccca ggaagagatg acgcatatgc agcgtctgtt
      181 tgattacctg actgataccg gcaatttacc gcgtattaat accgttgaat ctccgtttgc
      241 tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac aattaatcac
      301 ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc caacatttaa
      361 tttcctgcaa tggtatgttt ctgagcagca tgaagaagag aaactgttca aatcgattat
      421 tgataaatta agcctggcag gcaaaagcgg cgaaggtctg tattttatcg acaaagaact
      481 ctctaccctc gacacacaaa actaatgctc tccggcggca ggtttactgc cgccgtttca
      541 aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac cgtcttccga aggtataaat
      601 ttgccttttg ctgccagaaa cgctaccagc tctcccgcag tcatcccttc tgccgagcag
      661 gtgtgaaaac gtgcctgttc accaaaacgc gctttaatcg cagcttccag actggcatgc
      721 gtatattgct cgcctgattc aatcatcata tttaacactt catgaccgtg aatagagtcc
      781 atcgttcctc ctcaaaaaaa agcctagcgt agcgattacc gcttatgaag actttgcgcc
      841 agcgcaggac tgaatgcttt tattgtacat ttatatttac accatatgta acgtcggttt
      901 gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat cactcataaa gatcgtcagg
      961 acagaagaaa gcgtgaaaaa cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca
     1021 attggcgcag gcatgctggc aatgccgctg gctgcggcag gtgttggttt tagcgtcacg
     1081 ttaatcttgt tgattgggct ttgggcgttg atgtgctaca cggcgctatt actgctggag
     1141 gtgtaccagc atgttccggc agataccggt cttggcacgc tggcaaaacg ctatctggga
     1201 cgctacggtc aatggctgac gggcttcagt atgatgttct taatgtatgc tctgactgcg
     1261 gcatacatca gcggtgccgg tgaattgttg gcctccagca tcagcgactg gacaggtatt
     1321 tctatgtcgg caaccgctgg cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt
     1381 gtcggaactt cactggtcga tttatttaac cgttttctgt tcagcgccaa gattattttt
     1441 ctggtggtaa tgctggtact actgctgccg catattcaca aagtgaatct tttaaccctg
     1501 ccgttgcaac aggggctggc tctgtctgca atcccggtga tttttacgtc gtttggtttt
     1561 cacggtagcg tgccgagtat tgtcagctat atggatggca acattcgtaa gctacgctgg
     1621 gtgtttataa tcggtagtgc gatccccctg gtggcatata ttttctggca ggtggcgacg
     1681 cttggcagca ttgattcaac aacctttatg ggattgctgg ctaatcatgc tggattaaac
     1741 gggctgttac aggcgttacg cgaaatggtg gcctctccgc atgttgagct ggcagtgcat
     1801 ttatttgctg atttagccct cgccacgtca tttctcggcg ttgcgttagg cttatttgat
     1861 tatctggctg atttatttca gcgttcaaat accgttggtg gacggttgca aactggtgca
     1921 attacgtttc tgccgccgtt ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg
     1981 ctgggttacg ccggtgtggc gctggcggta ctggcattga ttatcccttc gctgttgacc
     2041 tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg
     2101 ctggtggtgg tgtttctctg tggtattgct gtgattggcg tgcaattttt gattgcggca
     2161 gggttgttac cagaagtggg gtgatcagat agcctcaaat tccttattgg gtgccagaat
     2221 taacgctgac acccaatttg gcctcttaat gcaggcagca ctgcttaaat ttcttaccac
     2281 taccgcacgg gcaaggatcg ttacgccccg gtttctcttc tgctttgatc ggttgctgaa
     2341 cagctttttc ctgcggatgc gccatccagt acgcatgtag atcaagcgcc gccagtcgaa
     2401 tggcatctac gctctcttca aacgcttctg gcgacatctt ttctacccgc tcgaagtttt
     2461 cctcagtacc gtgcagcgca atcgcctcca gcgctggttt taacgaatcg ggcaacgttg
     2521 accagtcaga aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca
     2581 gctcgctgcc atcaacttct cgcaagccga ataacggctc aaactgctcc gggaattcgt
     2641 tcagacgctc tgcggtatcg gccatatgtt gaaaagccag attcataaag cgcgtcatct
     2701 ctttctctga cgcccagcgc ggcacatagt cagccccacc ccacacggca accagccact
     2761 gttccggttc aatctcttgc ggagaactca acaccgccgt caataaaccg tccagctccg
     2821 ccacatcaag gatggcgtgg tcagtgttgt atttggtcag aatatcgtcc agccattcca
     2881 actcactttc gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc
     2941 agcacctatc ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgctggtga
     3001 ttaaaaatta gggagggtgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg
     3061 tttcacgaca ctgcttattg ctttgatttt attcttatct ttcccatggt acccggagcg
     3121 ggacttgaac ccgcacagcg cgaacgccga gggattttaa atcccttgtg tctaccgatt
     3181 ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg gagtcgaacc ggactagacg
     3241 gatttgcaat ccgctacata accgctttgt taacgcgcca aattcttcag gcctttcagc
     3301 cagacatccg cttgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg
     3361 cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcatcaagc
     3421 aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt
     3481 gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt gatcgtttgc
     3541 tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc gctcaaatat
     3601 tgcaacattg accacagagt cagtaccgca gccacaaaga aaagtgcaat accggcgtac
     3661 tcaacccaaa tgttcggacg ccacagcagc catgccaacg ccaccatctg ggcagtggtt
     3721 ttcactttcc caatccagga gacggccacg ctactgcgtt tacccaactc cgccatccat
     3781 tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg taatgtcacc
     3841 caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag aactttatct
     3901 gccacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa
     3961 ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac
     4021 caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac acggaacagt
     4081 gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga
     4141 ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca
     4201 gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta cgtaaacctt
     4261 gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg ccttcaatgg
     4321 tttccaggga actggtattt ttgaccttcg cccttttttt acggtgcccg ccaatcgcgt
     4381 gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa tctggcggca
     4441 aactaaatcc ctcaccttcc ggctcaaaga acagtgtttc cagcccagcc ttacgatctg
     4501 ctcctttggc aacgccaagt agcagtggat gatttttatc ccatgagaca tccagttcgg
     4561 cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg atcacatccg
     4621 ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga ttcatcgccg
     4681 cataatcatc gcccggcgtg atacctgtaa tgttatagcg ccgatactcc gcacgcagcg
     4741 ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg gtatggctga
     4801 tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca agcgcggtca
     4861 gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg
     4921 tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga acattaatct
     4981 tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat
     5041 cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactggccta
     5101 cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc
     5161 ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat
     5221 cgaacgccac accaataacg tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg
     5281 tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc agcttcttca aactccagat
     5341 tctggctggc agtttccata cgactaatga gttgcgtaag cacctgatca tctttgccag
     5401 acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt
     5461 caacgcacgg tcccagacag cgccctatct ggtattgcag acacggacgc gagcgattgc
     5521 gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt
     5581 cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca tgcttcgcac
     5641 cacgatgcat cgccagacgc gggtgggtat caccactcag gaagataaaa ggatatgatt
     5701 tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca
     5761 gcaacagcgc ttcggtttct gtgtgagtaa ccgttacatc aatttgctgg atctgggcga
     5821 ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa agccgttttt
     5881 tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa
     5941 cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg
     6001 acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac
     6061 atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca ccgttttcgg
     6121 actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga tcatcagcat
     6181 aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat
     6241 ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc ctgaatagac
     6301 agaacgaatc gcactcacaa cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc
     6361 accggcctgc atgactttcg ctggtaaagg gttttctgta tggacggtaa gcatgatgat
     6421 tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg
     6481 catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact taacggcgtc
     6541 ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca gaatgcgtcg
     6601 tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aaggaatatc
     6661 tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg
     6721 caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gtcaattcga
     6781 ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt cacagtaagc
     6841 cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat cagcccgctg
     6901 aatatgtaac attaattaac ccgaacaata cgggcaaaaa acattcatga taaaaatatt
     6961 tattggtcat tatattaacg tattttatag cactgccgat atcacgctca aaaaacaacc
     7021 actgctattt ttagcaaagc ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa
     7081 ttttcattgc aacatgacga ggaaaataaa tgagtacgcc tgatttttct actgccgaga
     7141 ataatcaaga actggcaaat gaagtctcct gcctgaaagc gatgctgacg ctgatgctgc
     7201 aggcgatggg acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc
     7261 tgatcgaaga cgaaacccag gctgcagtat tttccaaaac ggttaagcaa attaaacaag
     7321 cctaccgtca gtaatataaa accggctgag agcgtgcctt tcagccggtt tttgcatctg
     7381 gcacgcagaa agaaaagcga tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg
     7441 gtcttatttg gtgcattgat ttttttctgc atgtttttct gatggaaatt gaccgtattc
     7501 tcagagattg acaaaatcat cgctatctct gctgatgtct tcccttccgc cgtccatttc
     7561 agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt
     7621 aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa ttgcagttca
     7681 tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc
     7741 cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gcgcgcggct
     7801 tcccataacg gctgtgcttc gctgaataag tcatcattcc acattaaatg gccctgacta
     7861 aagttttcag ggttcagcac cggatcaatt gcgagaaagt tttttgcctg ataataacta
     7921 acccacgcct cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg
     7981 tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag ctctatttca
     8041 tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt gcgacgccag
     8101 ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta
     8161 taatgataat tcaaactatc acataaataa gatttatata taatttatat tattcaggca
     8221 atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta tgctgatttg
     8281 ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat cgtcggggct
     8341 gattttttct tattattgca gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg
     8401 gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct ggtccataaa
     8461 aatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt
     8521 gcgcttttcc tgcgccagct gacggatggt gttcaggact tcgcccacca gctctggatc
     8581 cagcgctgac gttggctcgt caaacaaaat cacctcagga cgcattgcca gcgcacgcgc
     8641 aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg tttctttacc
     8701 ggccagccca actttcgcca gtagctcgcg agcgcgcgcc gtggcctctt ctttcggttc
     8761 gcctttgacg atcaccggcc cttcaataat gttctccagc accgtacgat gcggaaacaa
     8821 attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg
     8881 ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg ttcccgcttc
     8941 gggttgttcc agcagattta tgctgcgtag caacgtggtt ttgccggaac cactcggacc
     9001 gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca gcaccgtctg
     9061 accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg ttctctctcc
     9121 tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc
     9181 cagtagatga gcgaagccgc cagatacatg gtgaacacct ccagcgtacg cgaggtaatc
     9241 aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt
     9301 accaggctga taaagctgtt cgacagcggc ggcaacgcca cacgcgccgc ctgtggcaaa
     9361 atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggctgccgc ttcccactga
     9421 cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cagcataggc ggcagtgttc
     9481 aacgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa ctgcggcagg
     9541 ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat agagatataa
     9601 aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc gagaataaac
     9661 ccgagcagta agccaaaaaa catgccgccg atactgagtt gcagcgtata cccagcacct
     9721 ttcagcagga acggcaagga atcaataacc agttgtatac tttcttgcat gagcgtttcc
     9781 ggagctaaac gtggggatga taggcgaaca gcgcaggcgc accgccggta tgaataaaca
     9841 gaatcggccc ttcatctttg aaacgtttct gactgatacc gtcaatcaga cccgccatcg
     9901 cttttccggt atacacagga tcaagcagaa tgccttccag ccgcgccagc aatttcactg
     9961 cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca tcccagagta
    10021 aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt aggttaacca
    10081 ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt tcgctttcag
    10141 gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg cccgatgcga
    10201 ccaccaccga cgaaatatta accgcccctt cacactgttg cgcaatttcc agcgcactct
    10261 ccacataacc cagcgcgccc agagcattag aaccgccaac cggaatgaca tacgggcgaa
    10321 agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga tcggtcagtg
    10381 cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg ccgttggtta
    10441 aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag tgcagaccga
    10501 gtttcgccgc aactgcggca gtctggcgca catggttaga ctggatcgcc ccggcagtaa
    10561 tcagcgtatc ggcaccttca cgcagagcat ctgccgcgag aaattccagc ttacgtaatt
    10621 tattgccgcc cattgccatg ggtgtgacgt catcccgttt gatgaaaatt tcccgtccaa
    10681 gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata aactccagcc
    10741 gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt
    10801 ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt
    10861 ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg aaagggcttg
    10921 cagagtgcca tctttttgca tttccgcaat tgcatcattc actgctttca gcaggtcttc
    10981 atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac cggttactgc
    11041 cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacggtcaa cgaggatcgc
    11101 atcgatacgg cctacgcgca gatcctgata tttggtcggg tcatcatcat aggtacgcac
    11161 atcgacgccc tgaacattct gacgcagcca ctcttcatag ttggtgccca ggccgacacc
    11221 cacttttttg cctttcagat cagcagctgt tttaatggtg ccttcgttac cttttttcac
    11281 cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt tcttgcgctc
    11341 gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca gcgacgccag
    11401 cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt ttgccagctg
    11461 ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tctccctgaa aactgaacgg
    11521 cggataagtt ccttccagcc ctaccagcag cgtgccgcgc tctttaactt tattaagcag
    11581 accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgg ccatcacacc
    11641 catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg ttgttatgtc
    11701 tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac atcttattct
    11761 tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc cggtacaggt
    11821 ttggcgctga tagtgttgat acttccgtaa cgcgatgcgg taattgttgg tgctggtggt
    11881 gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat
    11941 attttgctcg tgtagatgat tccccaaacg gcgcagacga acgacatatt cacgcaccgt
    12001 acttgggctc atttcagttt gctcaaaaag aaattgctta aagccgataa tatcgaaaaa
    12061 atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg cccatgattt
    12121 ttgttccgtt tgccagccct tttcatcaag cagcgtgtcc aggcgggaaa tctcaatttt
    12181 attcactatt ttgccgtcgc gaacgagggt aatgcgatcg agtaatttac ggcaatgcgc
    12241 gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc gtcttttcag
    12301 gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca tttactgacg
    12361 ttataactta cccagtttag tgcgtaaccg tttaatagcc tggctgtgta actgactgac
    12421 ccgcgattcc ccgacctcca gcactgcacc aatctctttg agattcagct cttcctggta
    12481 atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg
    12541 ctggcgcaga ttattgtcca gcagttgttg tagcgggttt tctcgctgat gatcatcagt
    12601 aaccagttcg atgctatcgc cgtgctcttc gcgccactca tcgtaggaga agagctggct
    12661 gttattggtg tcgagcaaca tttggcgata atcggcaata tcgatcccta aacgttccgc
    12721 tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc
    12781 cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc gaagttcatc
    12841 cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc cttgtagggc
    12901 gtcatagcgt tcgacggcat taagtaaccc aatgccgccc gcctgtagca gatcgtcaag
    12961 ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac
    13021 ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga gtgaattcac
    13081 gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg cagggttttt
    13141 aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttaccggta aattgcggac
    13201 agaaaaaacc ccgccggtgg cggggaagca cgttgctgac aaattaccat tcatgttgcc
    13261 ggatgcggcg taaacgcctt attcggccta caaaaatgtg caaattcaat aaattgcaat
    13321 tcaccttgta ggcctgataa gcgcagcgca tcaggcaatt tggcgttgcc gtcagtctca
    13381 gttaatcagg ttacgacgat taaccctgca gcagagacag aacctgctgc ggtacctggt
    13441 tggcttttgc cagcacggag ttaccggcct gctggatgat ctgcgctttc gacatattgg
    13501 acacttcggt cgcatagtcg gcgtcctgaa tgcgggactg cgcttcagac aggttggtag
    13561 tggtgttgtt caggttggtg actgcggaat ccagacggtt ctgcaccgca ccgagggagg
    13621 agcggaattt gtctacggat gcgatagcat cgtccagcgc tttcagcgga tccgtggtca
    13681 cttcggattt accagtagct tcagtggtca gagaaccgtc agccgctttg tacagagccg
    13741 cgccgtcatc atccagaata ttgccgttag caaattcgtg ataagtttct gtgacagtct
    13801 tgttggtcgc agtcgtcagt tcaccatcag caccaatatt tacggtagcg ccacctgtag
    13861 tagcggttac tgccccacca gcggcaacgt caaaagactg tgcgccgtta gctacggtat
    13921 aagcctgccc agttaacgca gtgtccatat cttcggcgga taccgttgcg cccttaatat
    13981 ccactttttt gctagcctgg gtgaaatcaa cggtcatgcc gctgccgaat gtaatcttcg
    14041 cattaactgc tgcattacca ttcgcaccgt tgaaaatacc gtccaacgtt gccgcatctg
    14101 gaccgccggc ctggttttta gtcaggttgc cgtcagaacc aatatataaa gcatcaccat
    14161 tgacgtcagt cagggtgcca tcgctggcaa ttttaacgtc ctgagcgctt ccgccgatgg
    14221 agatctgagc cgtattatca ccgtttgctg cataggcttt cagcacatca gcaactttag
    14281 tggtggcccc agtaccatcg ccattggtaa gtgcggttgc gtcaaatgaa taagacttgc
    14341 tggttgcgtt gtaggtatat gcagtagaag ccgctacgcc taatccattg tttgtaccgg
    14401 tataaccgct aatagtgctg ccgccgctaa cgcttgccag cacgtcggta gctgttgctg
    14461 cattaaaggt agtaacagta gatttagtcc aggtgctgtt gccactgacg actgcacctt
    14521 gggagaagcc cgcagcggtc agatctgctt cagtggcttt agcattatca acagcgcctt
    14581 taccgttaac attgaagctg gtcagcttca atgtagaaga gtcgatcttt ttcagatcaa
    14641 tcgtgatggt ttcaccatca tttgcgccga cctgaatttt catcgagcca tcttttgaca
    14701 gtacgttcac gccgttgaac tgggtttggc cggatacgcg gtcaatttca tcaagacggg
    14761 atttgatttc gtcctgaatg gagtccaggt cagaggtgga gttagttccg gtcgtcgcct
    14821 gaaccgtcag ttcacgaata cgctgtaagt tgttgttgat ttcggacagc gcgccttcag
    14881 tggtctgtgc aacagaaata ccgtcgttgg cgttacgtgc agcctgagtc aggcctttaa
    14941 tgttagaagt aaaacggtta gcaatcgcct gacctgcggc gtcatccttc gcgctgttaa
    15001 tacgcaagcc agaagacaga cgctcgatag aactcgacag cgcagactgg ttcttgttga
    15061 tattattttg agtgatcagc gagaggctgt tggtattaat gacttgtgcc atgattcgtt
    15121 atcctatatt gcaagtcgtt gattacgtat tgggtttcca cccgtcggct caatcgccgt
    15181 caaccctgtt atcgtctgtc gtaaaacaac ctttagaatt tttttcacaa acagccattt
    15241 tttgttagtc gccgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa
    15301 ttaaacgtaa actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa
    15361 agataaaagg aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt
    15421 taagttcaat ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cccatttcaa
    15481 atcagcaatc gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga
    15541 cgactttcca gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca
    15601 ctaccagcag caccaccgca ttcagtgcca ccactgcggg taacgccatc gccgggaaat
    15661 acaccatcag tgtcacccat ctggcgcagg cgcaaaccct gaccacgcgc accaccagag
    15721 acgatacgaa aacggcgatc gccaccagcg acagtaaact caccattcaa caaggtggcg
    15781 acaaagatcc gattaccatt gatatcagcg cagctaactc gtcgttaagc gggatccgtg
    15841 atgccatcaa caacgcaaaa gcaggcgtaa gcgcaagcat cattaacgtg ggtaacggtg
    15901 aatatcgtct gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg
    15961 tcagcggtga tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg
    16021 gtatggaggt ctcggttgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg
    16081 agaacagcag caacaccatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg
    16141 tcaccacggg caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga
    16201 ttaaagactg ggtgaatgcc tacaactcgc taatagatac cttcagcagc ctgaccaaat
    16261 acaccgccgt agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg
    16321 actccacgct gcggacgatt cagacgcagt tgaaatcgat gctgagtaat accgtcagtt
    16381 cttccagcta taaaacgttg gcgcagattg gtatcacgac cgatcccagc gatggcaaac
    16441 tggaactgga tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg
    16501 cattgattgt tggcgatggt aaaaaaaccg gcatcacgac caccatcggc agcaacctga
    16561 ccagttggct ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaagaccc
    16621 tgaataaatt aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgctc
    16681 gctacaaaga acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca
    16741 gctacttaac gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga
    16801 gacaggtcat gtacgcggca aaaggcaccc aggcctatgc acaaattggc gtcgaaagcg
    16861 ccgtaatgag cgccagtcaa cagcagctgg tcaccatgct atttgatgga gtgctgagcg
    16921 cactggttag agcgcgcctg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt
    16981 tgtcaaaagc gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca
    17041 aagacgaatt aacccaaaac ttgattgctc tttatagcta tatggtcagg cgcttgctgc
    17101 aagccaattt acgcaacgat gtctccgcag tcgaagaagt ggaagcatta atgcgcaata
    17161 ttgccgatgc ctggaaagag tctttactct ccccttcttt gattcaggac ccagtctgat
    17221 gaaccatgca ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat
    17281 gttacgcctg gcaacggaag aacaatggga cgaactcatc gccagcgaaa tggcgtatgt
    17341 gaatgcggtg caggagattg cacatctgac tgaagaggtt gacccgtcca ccacgatgca
    17401 ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg taaagcagtt
    17461 attacagatt cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc
    17521 ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt
    17581 ttgaatctga acgagttgat ggctcgcgaa taatccgatt acggctacgc ttctaatgtt
    17641 ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat
    17701 tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg ttttaatgat
    17761 attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg cgggtattcg
    17821 gtcggctacg actcctatga tttatttgat ttaggcgagt ttgatcagaa aggcagcatc
    17881 cctactaaat atggcgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat
    17941 gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa
    18001 gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt
    18061 gagtgtgaag gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt
    18121 atctgggatt tcaaatgttt tagcggtatc gaccatatcg aaaaccctga tgaagatggc
    18181 atatttaaaa ttgttaacga ctacaccggc gaaggctgga acgatcaggt tgatgatgaa
    18241 ttaggtaatt tcgattatct gatgggcgag aatatcgatt tccgcaatca tgccgtgacg
    18301 gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggttttcgt
    18361 cttgatgcgg tcaaacatat tccggcgtgg ttttataaag agtggatcga acacgtacaa
    18421 gaagttgcgc caaagccgct gtttattgtg gcggaatact ggtcgcatga agtcgataaa
    18481 ctgcaaacgt atattgatca ggtggagggc aaaaccatgc tgttcgatgc gccgctgcag
    18541 atgaaattcc atgaagcatc gcgcatgggg cgcgactacg acatgacgca gattttcacg
    18601 ggtacattag tggaagccga tcctttccac gccgtgacgc tcgttgccaa tcacgacacc
    18661 caaccgttgc aagccctcga agcgccggtc gaaccgtggt ttaaaccgct ggcgtatgcc
    18721 ttaattttgt tgcgggaaaa tggcgttcct tcggtattct atccggacct ctacggtgcg
    18781 cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa
    18841 cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacgttattt
    18901 ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ctgacgaata tcccggctgc
    18961 gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg agagaattac
    19021 ggcaataaaa cctggcgtga ttttctgggg aaccggcaag agagagtagt gaccgacgaa
    19081 aacggcgaag caaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag
    19141 gtgatttaaa ttcatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg
    19201 taacggcgct ttatccagcg cggcggcaca ctctgcggtc gggcgatcca cacgctccat
    19261 cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt
    19321 tacggtaacg ttagtgagat catcagacat caatgtcaac ttgcccggca cagcaatcac
    19381 ccgctgccac tgacggcaat caagagtatc gccctcttta gtgacaatca ggctgcctat
    19441 cgcttccgga ctgaccagcg cacgttgcgg tcctttggtc tgccagtagc ccgccagcca
    19501 gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag
    19561 caacatgagc gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa
    19621 gaggattgtg gcattaaagc cctgatgtcg ccagcctttc aaagttcaga gagaaatttg
    19681 atctaactat tatttttgtg aattattacc ccgaaaataa atttgcttga tgtatgattc
    19741 gcgactcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc
    19801 aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt
    19861 ctctccactt actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc
    19921 cgttggggcg gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt
    19981 aaaattatcc atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggt
    20041 atgtttggcg gctgctttgc cgcagcgctg tgggccaaca atgtcaaact acgcatgccg
    20101 cgcagccgta tccgcattat gcaggccatt attggcggca ttatcgccgg ttttggcgcg
    20161 cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg
    20221 catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc
    20281 ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg
    20341 acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatggtggt ctttttcggc
    20401 atgctgggct gggcgctgct cacggcgatg aaccaaccca aactggggct ggcaatgctg
    20461 tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc
    20521 cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg
    20581 gtaagtgcca tcgggatctt cagttacgta cagttaggtg ttgaacccaa aatcatgtgg
    20641 gcgggaccaa acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggcaggc
    20701 ggctgcgaaa ccggttggat gtaccgcgcg gtagaaggtc aggtgcacta ctggtgggtc
    20761 ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt actgggatga tttcgctccg
    20821 gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cctttggccc gatgggtggc
    20881 ctgctggtga catatttgct gttgtttact gcgctgatgt tgattatcgg ctgggaaaaa
    20941 cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc
    21001 ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaactcttg
    21061 aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccacagt
    21121 cgatcaataa tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc
    21181 aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc
    21241 acagcgggaa catcgttgcc tctgacacat agattttacc cgccccctat cctattcttt
    21301 tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa
    21361 gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc
    21421 attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa
    21481 caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg
    21541 ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc gctctggcaa
    21601 catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa
    21661 aaacagcctt attttatcta tcgcgctgat ggacaacctg tttttatggc cgcgataggt
    21721 agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac tgcagcagca
    21781 gaccagggtc tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc agaagctgca
    21841 caggaatgga tgcggcaaga gattagcggt aaagaagcct cagaaatcgc ggtcagtggt
    21901 tgtgtgccag caaagcagtt ctcctggcat ccggtattgc gcgcggtggg taatgtcaaa
    21961 aaccagggcg cagcgttaat tcaacctgtc tgttaaccct ggagattatt gatgaactaa
    22021 aatgtgtcac aagaaacctc tattccagtg acacaattgc gcctaattac ttacatataa
    22081 tatttaatta tgagttcctc accatctatt acatactttt taaccatatc ggaatattta
    22141 tcataatcag cgggattcgt
//
LOCUS       DAFAVF010000063        21649 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFAVF010000063 DAFAVF010000000
VERSION     DAFAVF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.063"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..596)
                     /locus_tag="LGK42_002403"
     CDS             complement(<1..596)
                     /locus_tag="LGK42_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK2658643.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="LGK42_002404"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="LGK42_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK2658644.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="LGK42_002405"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="LGK42_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK2658645.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="LGK42_002406"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="LGK42_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK2658646.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="LGK42_002407"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="LGK42_002407"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBK2658647.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="LGK42_002408"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="LGK42_002408"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBK2658648.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="LGK42_002409"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="LGK42_002409"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBK2658649.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /locus_tag="LGK42_002410"
     CDS             complement(4985..5707)
                     /locus_tag="LGK42_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2658650.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQSDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSNVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFLGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="LGK42_002411"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="LGK42_002411"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2658651.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /locus_tag="LGK42_002412"
     CDS             6907..7125
                     /locus_tag="LGK42_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBK2658652.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="LGK42_002413"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="LGK42_002413"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK2658653.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /locus_tag="LGK42_002414"
     CDS             complement(7859..8059)
                     /locus_tag="LGK42_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBK2658654.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="LGK42_002415"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="LGK42_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBK2658655.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="LGK42_002416"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="LGK42_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBK2658656.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /locus_tag="LGK42_002417"
     CDS             10556..12469
                     /locus_tag="LGK42_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2658657.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /locus_tag="LGK42_002418"
     CDS             12469..13491
                     /locus_tag="LGK42_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2658658.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /locus_tag="LGK42_002419"
     CDS             13485..13703
                     /locus_tag="LGK42_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBK2658659.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /locus_tag="LGK42_002420"
     CDS             13757..14626
                     /locus_tag="LGK42_002420"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBK2658660.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /locus_tag="LGK42_002421"
     CDS             complement(14681..15085)
                     /locus_tag="LGK42_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBK2658661.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="LGK42_002422"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="LGK42_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBK2658662.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /locus_tag="LGK42_002423"
     CDS             16070..18160
                     /locus_tag="LGK42_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK2658663.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD/dapC"
                     /locus_tag="LGK42_002424"
     CDS             complement(18227..19447)
                     /gene="argD/dapC"
                     /locus_tag="LGK42_002424"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBK2658664.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGELFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVIGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="LGK42_002425"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="LGK42_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBK2658665.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /locus_tag="LGK42_002426"
     CDS             complement(20128..20730)
                     /locus_tag="LGK42_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBK2658666.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /locus_tag="LGK42_002427"
     CDS             complement(20720..20887)
                     /locus_tag="LGK42_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBK2658667.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="LGK42_002428"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="LGK42_002428"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBK2658668.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
BASE COUNT         5336 a         5906 c         5358 g         5049 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga gaacggctca tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat aatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacaccatct
     4141 tgcaataata gcagttcgtc gccctcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa tgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagcg ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcccca agtccgctta aggtaaatcc tggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcgg ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc aggaagtcgc cacttttgaa ctggcgaata tagagataaa
     5041 cagtatgttt ggaaatattc agacgatcag caacctgatt aatcgcgtct tttatgtcga
     5101 atatcccttt ctcgtagaga ttcaatacga tctggcgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcta
     5221 cagaggaagc aaagttgaca tttgagccga catccggcgt ctctggtggc acgaaggtgc
     5281 tcataatctg cgagaaagga acatcgaggt tcatattaat gcacagcagg ccaattacac
     5341 gctgttcgcg attacgaatg gcgatggtca gcgacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtga agcatacgta
     5461 gcgccaggtc ggtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatac
     5521 gaatggctga acattttaaa tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 tgagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcgctct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggaccct ggtcagtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacaa cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgagattga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaacgtac cgagcgccat cgacagcccc agcgcgtcca
     9181 taaacaatgc ggaacccaac accaacagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaaacgg aataccggac gcagtaaata gcgcccgcca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagaa
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc agttgaagac atcgcaaggc caatgccacc gacaaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagttt ggagggattc aactcaagac
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc catggaccaa ttgcaattcc tgccagcaaa tatcccaaca
     9781 cagcgccaat acccagccgc gatgccagcg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg actcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacatcgc tcatcgggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtaa tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc gccttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt atgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctga ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc aggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cgctgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg cgtggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat gcgcggcttt
    12541 agcaattgtc atctacaaac tatgctgccg cgtttgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12661 gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct ggaaggcagt
    12721 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgcta
    13081 aacctgttaa aagccaatgc cgcacgcaag ctggcagcct accccggaac actgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct aatcaccgcc
    13201 agaattcacg gctacgctga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc tacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg ttgagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cggtagtgcg ttcaatggaa gattatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcag
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatagaaacc acatcgatgg
    14941 cactgcaacc ccccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgcttg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct cactcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttgctgaca cagctactac tggcaaaaga
    16321 tgttcctctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagct
    16381 ggggccattg cacgctaaat tgcttcccgc atcgctgctc gccgccattt ttaccctcag
    16441 tctggcggga tatatgccgg tctgggaacc attgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa actggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tattggcact tcagccgcat
    17041 tgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccgttacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacgc aaaatggcta
    17281 tggcgcaacc cgtttgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacct tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 taccattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc agtacatcct
    17521 tccacgtctt atcgatacca ttattggttg tttgattgct ttcggtggta ccgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgatgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttctgagcga ggacccgcaa cctaccccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccagcg ttcaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcggctggaa tatgacgaac cggggagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaatca
    18241 ccttagccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgaa ctgtggctta agctccgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acaccgtagt
    18721 gcatgtaagc aaacaattcg ccagtccgcc ccatcccgca ctgcacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgcag
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa agggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacacagg
    19081 cataatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcattcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcccg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcactgaat ggcaatcaga aatcagcgat gcagaaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaatt gcacaccttc cagatcccac
    19621 tggcgatgac gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcac
    19681 gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt gagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcata
    19801 acctttgcgg cgcgcacaac tttaccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgct ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
    20521 aatatcctgg tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg cggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgactcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctgaaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcaaaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc ggcggtacgc gccatcgcga tggtaccacg
    21241 cgtgttgcgc aggccattat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttctgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttaccta
//
LOCUS       DAFAVF010000064        21550 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFAVF010000064 DAFAVF010000000
VERSION     DAFAVF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.064"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            49..1092
                     /gene="nadA"
                     /locus_tag="LGK42_002429"
     CDS             49..1092
                     /gene="nadA"
                     /locus_tag="LGK42_002429"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBK2658669.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1130..1849
                     /gene="pnuC"
                     /locus_tag="LGK42_002430"
     CDS             1130..1849
                     /gene="pnuC"
                     /locus_tag="LGK42_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBK2658670.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(1846..2787)
                     /gene="zitB"
                     /locus_tag="LGK42_002431"
     CDS             complement(1846..2787)
                     /gene="zitB"
                     /locus_tag="LGK42_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBK2658671.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(2901..3281)
                     /locus_tag="LGK42_002432"
     CDS             complement(2901..3281)
                     /locus_tag="LGK42_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBK2658672.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3597..4649
                     /gene="aroG"
                     /locus_tag="LGK42_002433"
     CDS             3597..4649
                     /gene="aroG"
                     /locus_tag="LGK42_002433"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBK2658673.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4807..5559)
                     /gene="gpmA"
                     /locus_tag="LGK42_002434"
     CDS             complement(4807..5559)
                     /gene="gpmA"
                     /locus_tag="LGK42_002434"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK2658674.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5764..6804)
                     /gene="galM"
                     /locus_tag="LGK42_002435"
     CDS             complement(5764..6804)
                     /gene="galM"
                     /locus_tag="LGK42_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBK2658675.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6798..7946)
                     /gene="galK"
                     /locus_tag="LGK42_002436"
     CDS             complement(6798..7946)
                     /gene="galK"
                     /locus_tag="LGK42_002436"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK2658676.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(7950..8996)
                     /gene="galT"
                     /locus_tag="LGK42_002437"
     CDS             complement(7950..8996)
                     /gene="galT"
                     /locus_tag="LGK42_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBK2658677.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFREFGV"
     gene            complement(9006..10022)
                     /gene="galE"
                     /locus_tag="LGK42_002438"
     CDS             complement(9006..10022)
                     /gene="galE"
                     /locus_tag="LGK42_002438"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK2658678.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10283..11755)
                     /gene="modF"
                     /locus_tag="LGK42_002439"
     CDS             complement(10283..11755)
                     /gene="modF"
                     /locus_tag="LGK42_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBK2658679.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(11823..12611)
                     /gene="modE"
                     /locus_tag="LGK42_002440"
     CDS             complement(11823..12611)
                     /gene="modE"
                     /locus_tag="LGK42_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBK2658680.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12740..12889
                     /gene="acrZ"
                     /locus_tag="LGK42_002441"
     CDS             12740..12889
                     /gene="acrZ"
                     /locus_tag="LGK42_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBK2658681.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13056..13829
                     /gene="modA"
                     /locus_tag="LGK42_002442"
     CDS             13056..13829
                     /gene="modA"
                     /locus_tag="LGK42_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2658682.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13829..14518
                     /gene="modB"
                     /locus_tag="LGK42_002443"
     CDS             13829..14518
                     /gene="modB"
                     /locus_tag="LGK42_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBK2658683.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14521..15579
                     /gene="modC"
                     /locus_tag="LGK42_002444"
     CDS             14521..15579
                     /gene="modC"
                     /locus_tag="LGK42_002444"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBK2658684.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15580..16398)
                     /gene="ybhA"
                     /locus_tag="LGK42_002445"
     CDS             complement(15580..16398)
                     /gene="ybhA"
                     /locus_tag="LGK42_002445"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBK2658685.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16553..17548
                     /gene="pgl"
                     /locus_tag="LGK42_002446"
     CDS             16553..17548
                     /gene="pgl"
                     /locus_tag="LGK42_002446"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBK2658686.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17589..18542)
                     /locus_tag="LGK42_002447"
     CDS             complement(17589..18542)
                     /locus_tag="LGK42_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2658687.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            18726..19778
                     /locus_tag="LGK42_002448"
     CDS             18726..19778
                     /locus_tag="LGK42_002448"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBK2658688.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            19854..21287
                     /locus_tag="LGK42_002449"
     CDS             19854..21287
                     /locus_tag="LGK42_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBK2658689.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         5120 a         5300 c         5607 g         5523 t
ORIGIN      
        1 tacgctaatg tcggttttaa cgttaagcct gtaaaacgag atggtaagat gagcgtaatg
       61 tttgatccag acacggcgat ttatcctttc cccccgaagc cgacgccgtt aagcattgat
      121 gaaaaagcgt attaccgcga gaagataaaa cgtctgctaa aagaacgtaa tgcggtgatg
      181 gttgcccact actataccga tcccgaaatt caacaactgg cagaagaaac cggtggctgt
      241 atttctgatt ctctggaaat ggcgcgcttc ggtgcaaagc atcccgcttc tactttgtta
      301 gtcgctgggg tgagatttat gggagaaacc gccaaaattc tcagtccgga aaaaacaatt
      361 ctgatgccga cacttcaggc tgaatgttca ctggatctcg gctgccctgt tgaagaattt
      421 aacgcatttt gcgatgccca tcccgatcgt actgtcgtcg tctacgccaa cacttctgct
      481 gcggtaaaag cgcgcgcaga ttgggtggta acttcaagca ttgccgtcga acttattgat
      541 catcttgata gtttgggtga aaaaatcatc tgggcacccg acaaacatct ggggcgttac
      601 gtgcaaaaac agacgggtgg agacattcta tgctggcagg gtgcctgtat tgtgcatgat
      661 gaatttaaga ctcaggcgtt aacccgcttg caagaagaat acccggatgc tgccatactg
      721 gtgcatccag aatcaccaca agctattgtc gatatggcgg atgcggtcgg ttccaccagt
      781 caactgatcg ctgctgcgaa aacattgcca catcagcggc ttatcgtggc aaccgatcgg
      841 ggcattttct acaaaatgca gcaggcagtg ccagataaag agttactgga agcaccaacc
      901 gcaggtgagg gtgcaacctg ccgcagctgc gcgcattgtc cgtggatggc catgaatggc
      961 cttcagtcca tcgcagaggc attagaacag gaaggaagca atcacgaggt tcatgttgat
     1021 gaaaggctgc gagagagggc gctggtgccg ctcaatcgta tgctggattt tgcggctaca
     1081 ctacgtggat aacgaataat aaggcgtaac gttacgcttt gggggaaaga tggatttttt
     1141 tagtgtgcag aatatcctgg tacatatacc aataggggca ggcggttatg atctctcatg
     1201 gatcgaagcg gtaggcacga tcgccgggtt gctgtgtatt ggccttgcca gtctggagaa
     1261 gatcagcaac tacttctttg gcctgatcaa cgtcaccttg tttggcatta ttttctttca
     1321 gattcagctg tatgccagcc tgctattaca ggtgtttttc tttgccgcga atatttacgg
     1381 ttggtatgcg tggtcgcgac aaaccagtca gaacgaggcg gagttgaaaa ttcgctggtt
     1441 gccattgccg aaggcactca gctggttggc ggtttgcgtt gtttcgattg gtctgatgac
     1501 ggtatttatc aatccggtgt ttgcattttt gacccgcgtg gcagtcatga tcatgcaagc
     1561 attaggatta caggttgtga tgcctgaact gcaaccggac gctttcccgt tctgggattc
     1621 atgcatgatg gtgttatcta tcgtggcaat gattctgatg acgcgtaagt atgtggaaaa
     1681 ctggctgttg tgggtgatta ttaacgtgat tagcgtcgtt atttttgcac ttcagggcgt
     1741 ttacgccatg tctctggagt acatcatcct gacctttatt gcgctcaacg gcagccggat
     1801 gtggatcaac agcgcacgtg aaagaggctc acgcgcgctg tcccattaat ggtgatgatg
     1861 tgaatggcca gacacccctt cattcaggtg gcagtctggc ccatgacaag gttgatattc
     1921 catctgaatg gtggcgtgct caatctgata gtgatccatc aggtagtgtt ggatctgatc
     1981 caacaaggca tcgtgatcgt gtggcgggat cacctgcaca tgcaacgtca tcaccggctt
     2041 ctcgcctacc atccatacat gtacatggtg cacattgcgg acttccggga tttcccggca
     2101 catacgacgc ttcagttcag cgatatccag cgataccggt gcaccttcaa gtaattcatt
     2161 cacgctatct ttcaacagtc gccacgcgct acgcagaacc agaagcgaca ccagtatcga
     2221 gagaatgggg tcagcaggtg tccagccagt ccagataata atcaacgcgg caataatagc
     2281 cccaaccgaa cccagcaggt ccccgagcac atgcagtgcc gctgctcgta cgttgaggtt
     2341 tttctcttcg ctgccgtgat gaagtaacca gaaagaaagt atatttgcca gcaacccagc
     2401 cacggcaatt gccatcatca tgccgccctc gaccggacgc ggcgtgcgga agcgttctat
     2461 cgcctcccag acaatcaaaa tggtaattac cacgagggct atggcattca caaacgccgc
     2521 gagcgtggtc agtcttagcc aaccgaaagt gtggcgaatg gtgggaggac gacgtgaaaa
     2581 ttgcacggcg agtagggcga aaagcagggc ggcagtatcg gtcaacatat gacccgcatc
     2641 ggccagcaat gccagagaac cagaaagaaa accaccaacg acttctacca gcataaaccc
     2701 ggcagtcacg ccgaaagcat acaacaagcg acgagcatta ttatcttctg gcaggtgtga
     2761 ggacgtgtgt gagtgtgagt gcgccatgac gagtcatccc ttaattattc tcacttaact
     2821 ttatgacatc atactgcttt tagaagtgaa aaattaaaag ggagagactc cgctctccca
     2881 ttattggcta ttttgcaggg ttactgcgtg gtaccgtcgg ttttggtatc gacatcatta
     2941 ttgatgccat caccggtttg taccttttta ttgatatccg gacagcgacc atctttgcac
     3001 atggtgttct tgtgctcttc atctttggtc attccgtcat tgttcattga agaaccatcc
     3061 gaatgcagca ttgtgccacc agaaccggta tttaccccgt tattgtcgac gttatttggc
     3121 gcgacatttt cacgggcgtc aggggctacc tggcccgcat cagctgcggc gtttgcctgg
     3181 ccgttattag tttgcgctcc gctatcggca gccagtgcgg caccgctggc aaggcttaga
     3241 gtggcagtca gaaataatgt ggccagtttt gtcattttca taggatgctc ctgttatggt
     3301 cgttatgtcg gataacctct tccaacagtg catttgcagg tgaatataag gcattggttt
     3361 aagatttcag ccaggttatg aaacgcagca gagaatcttg aaataattaa caaacaaagg
     3421 agttacagtt agaaattgta ggagagatct cgtttttcgc gacaatctgg cgtttttctt
     3481 gctaattcca ggattaatcc gttcatagtg taaaaccccg tttacacatt ctgacggaag
     3541 atatagattg gaagtattgc attcactaag ataagtatgg caacactgga acagacatga
     3601 attatcagaa cgacgattta cgcatcaaag aaatcaaaga gttacttcct cctgtcgcat
     3661 tgctggaaaa attccccgct actgaaaatg ccgcgaatac ggttgcccat gcccgaaaag
     3721 cgatccataa gatcctgaaa ggtaatgatg atcgcctgtt ggttgtgatt ggcccatgct
     3781 caattcatga tcctgtcgcg gcaaaagagt atgccactcg cttgctggcg ctgcgtgaag
     3841 agctgaaaga tgagctggaa atcgtaatgc gcgtctattt tgaaaagccg cgtaccacgg
     3901 tgggctggaa agggctgatt aacgatccgc atatggataa tagcttccag atcaacgacg
     3961 gtctgcgtat agcccgtaaa ttgctgcttg atattaacga cagcggtctg ccagcggcag
     4021 gtgagtttct cgatatgatc accccacaat atctcgctga cctgatgagc tggggcgcaa
     4081 ttggcgcacg taccaccgaa tcgcaggtgc accgcgaact ggcatcaggg ctttcttgtc
     4141 cggtcggctt caaaaatggc accgacggta cgattaaagt ggctatcgat gccattaatg
     4201 ccgccggtgc gccgcactgc ttcctgtccg taacgaaatg ggggcattcg gcgattgtga
     4261 ataccagcgg taacggcgat tgccatatca ttctgcgcgg cggtaaagag cctaactaca
     4321 gcgcgaagca cgttgctgaa gtgaaagaag ggctgaacaa agcaggcctg ccagcacagg
     4381 tgatgatcga tttcagccat gctaactcgt ccaaacaatt caaaaagcag atggatgttt
     4441 gtgctgacgt ttgccagcag attgccggtg gcgaaaaggc cattattggc gtgatggtgg
     4501 aaagccatct ggtggaaggc aatcagagcc tcgagagcgg ggagccgctg gcctacggta
     4561 agagcatcac cgatgcctgc atcggctggg aagataccga tgctctgtta cgtcaactgg
     4621 cgaatgcagt aaaagcgcgt cgcgggtaag gtttaattgt cggatgcgcc gtcagagtgg
     4681 cgtatccgat gaatcaccac aggcctgata agtcgcgcag cgtcgcatca ggcaatgtgc
     4741 tccattgtta gcaacaaaaa agccgactca cttgcagtcg gctttctcat tttaaacgaa
     4801 tgacgtttac ttcgctttac cctggtttgc aaccgccgct gctttcgctg cgatctcgtc
     4861 agcattaccc agataatagc gtttcagcgg tttgaaattc tcgtcgaact catacaccag
     4921 cggcacgcca gtcgggatat taagctcaag aatctcttct tcgctcatgt tatcaagata
     4981 tttcaccagc gcacgtaaag agttaccgtg tgcagcgatg atcacgcgct caccgctctt
     5041 catacgcggc agaatggttt cattccagta agggatcacg cggtcaatgg tcagcgccag
     5101 gctttccgtc agcggcagtt ctttttcgct cagtttcgcg taacgcggat cgtgacccgg
     5161 ataacgctca tcatctttag tcagttccgg cggagtcact gcaaaaccac gacgccactg
     5221 tttcacctgc tcgtcgccat acttttcagc agtttccgct ttgttcagac cctgcaacgc
     5281 accgtagtga cgttcgttca gtttccagga tttctcaacg ggcagccatg cctgatccag
     5341 ttcgtccagc acattccaca gggtatggat agcgcgtttc agcacagagg tgtaagcaaa
     5401 gtcaaagctg taaccttcct ctttcagcag cttacctgct gcttttgctt cgcttacgcc
     5461 tttctcagac agatccacgt cgtaccaacc ggtgaaacgg ttttctttgt tccactgact
     5521 ttcgccatga cgaaccagaa ccagcttagt tacagccata tacttactcc tcaaatcatt
     5581 ttttaatgat aataattctc attatattgc cgtgacgaag caacagcaat gcttacgcat
     5641 aaccatagcg aaaatagtgt cgcagtgtaa ggttgttgtg aatattgagt tgcaaatatg
     5701 tcggtgtttg ctggtgattt gagcaatata aggataaagc cctcattacg agggcttcag
     5761 gtgctactca gcaataaact gatattccgt caggctggaa tactcttcgc caggacgcag
     5821 gaagcagtcc ggttgcggcc attcagggtg gtttgggctg tccggcagaa actcgctttc
     5881 cagtgccagg ccttgccagt cggcgtaagg ttcagttcct cttgatggcg tgccgccgag
     5941 gaagttgccg gagtagaatt gcagagccgg agcggtggtg tagaccttca gctgcaattt
     6001 ttcatctgct gaccagacat gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa
     6061 gaatgcgtga tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa actcactggc
     6121 gatgattttg gcgctacgga aatcaaaaga cgttccggcg acagatttca gaccgtcgtg
     6181 cggaatgccg ccttcatcaa ccggcagata ttcgtccgcc agaatctgca acttgtgatt
     6241 gcgcacgtca gactgctcgc cgtcaagatt gaaatagacg tgattagtca tattcaccgg
     6301 gcaaggttta tcaactgtgg cgcgataagt aatagagata cggttatcgt cggtcagacg
     6361 atattgcacc gtcgcgccga gattacccgg gaagccctga tcgccatcat ctgaactcag
     6421 ggcaaacagc acctgacgat cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc
     6481 ttccgggccg ccgtgcagct ggttaacgcc ctgacttggc gaaagcgtca cggtttcacc
     6541 gtcaaaggta taacggctat tggcgatgcg gttggcataa cgaccaatag aggcccccag
     6601 aaacgcagcc tgatcctgat agcattccgg gctggcacag ccgagcagcg cctcgcggac
     6661 gctgccatcg gaaagcggaa tacgggcgga aagtaaagtc gcaccccagt ccatcagcgt
     6721 gactaccatc cctgcgttgt tacgcaaagt taacagtcgg tacggctgac catcgggtgc
     6781 cagtgcggga gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa
     6841 gtctctttaa tacctgtttt tgcttcatat tgttcagcga cagcttgctg tacggcaggc
     6901 accagctctt ccgggatcag cgcgacgata cagccgccaa atccgccgcc ggtcatgcgt
     6961 acgccacctt tgtcgccaat cacagctttg acgatttcta ccagagtgtc aatttgcggc
     7021 acggtgattt cgaaatcatc gcgcatagag gcatgagatt ccgccatcaa ctcgcccata
     7081 cgtttcaggt cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc gttttcagtc
     7141 aggatatgac gcacgcgttt cgccacgatt gggtccagct catgtgcaac agcgttgaac
     7201 tcttcaatgg tgacatcgcg cagagctggc tgctggaaga aacgcgcacc ggtttcgcac
     7261 tgttcacgac gggtgttgta ttcgctgcca accagggtac gtttgaagtt actgttgatg
     7321 atgacgacag ccacaccttt cggcatggaa actgctttag tccccagtga gcggcagtca
     7381 atcagcaagg catgatcttt cttgccgagt gcggaaatta gctgatccat gatcccgcag
     7441 ttacagccaa caaactggtt ttctgcttcc tgaccgttaa gcgcgatttg tgcgccgtcc
     7501 agcggcagat gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa
     7561 gaacttaacc cggcaccctg cggcacattg ccgctgatca ccatgtccac gccgccgaag
     7621 ctgttgttac gcagttgcag atgtttcacc acgccacgaa cgtagttagc ccattgatag
     7681 ttttcatgtg cgacaatggg cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg
     7741 gctgccatca cgcgaacttt acggtcatcg cgtggtgcac aactgatcac ggtttgataa
     7801 tcaatcgcgc agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc
     7861 acgcggccag gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga
     7921 gattgtgttt tttctttcag actcatttct tacactccga attcgcgaaa atggatatcg
     7981 ctgactgcgc gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg ggtctctgcc
     8041 agcatttcat aaccaaccat aaatttacgt acggtggcgg agcgcaacag aggcggataa
     8101 aagtgcgcgt gcagctgcca gtgttgattc tcttcgccat taaatggtgc gccgtgccag
     8161 cccatagagt aggggaagga acactggaag aggttgtcat aacgactggt cagctttttc
     8221 aacgccagcg ccaaatcgct gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg
     8281 gctttgggca gcagtagcgt ttcgaacggc caggcagccc agtaaggcac aacggctaac
     8341 cagtgttcgg tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg aacataatcc
     8401 accagcattg gtgatttctg ttcggcaaaa tattcttttt gcaggcggtc ttcgcgctca
     8461 gcttcgttag gcaggaagct atttgcccaa atctgtccgt gcggatgcgg gttagagcag
     8521 cccatcgccg cacctttgtt ttcaaagacc tgcacccacg ggtacgtttt tcccagctct
     8581 gcggtttgct cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct cagttctggc
     8641 aacgttttac tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg
     8701 caacgcatca aaggatcgtt gctttctggc gcatctggcg tgtcagacat caaggccgca
     8761 aagtcattag tgaaaacgta agtcctggtg taatcggggt ttttatcgcc tgtcacccgc
     8821 acattacctg cgcagaggaa gcaatctgga tcgtgcgcag gtaacacctg tttggctggc
     8881 gtttcctgcg ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc
     8941 ccggtgagcg ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc
     9001 gttccttaat cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc
     9061 atttcatcga gtgtgcgcgt tacgcgccag ttcagttcac ggtcggcttt gctggcgtcc
     9121 gcccagtagg ccggaaggtc gccctcgcga cgcggtgcaa aatgataatt aaccggtttg
     9181 ccgcaggctt tgctgaaggc attaaccacg tccagcacgc tgttgcctac gccagcgccg
     9241 aggttgtaga tgtgtacgcc tggcttgttc gccagttttt ccatcgccac gacgtgaccg
     9301 tccgccagat ccattacgtg gatgtaatcg cgtacgccag taccatcttc ggtcggataa
     9361 tcgttaccaa aaatcgccag cgagtcgcga cggcctacag caacctgggc gatgtatggc
     9421 atcaggttat tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg atgcgcgcca
     9481 accgggttga agtagcgcag cagggcaatg ctccagtccg gctgggcttt ttgcagatcg
     9541 gtgaggatct gttccaccat cagcttgctt ttgccgtaag ggctttgtgg tgtgccggtc
     9601 gggaagcttt caacgtatgg aattttgggc tgatcgccat aaacggtggc ggaggagcta
     9661 aaaataaagt ttttgacgtt agcggcgcgc atggcgctaa tcaggcgcag agtgccgttg
     9721 acattgttgt cgtaatattc cagcggtttt tgtaccgatt cgccgacggc tttcagcccg
     9781 gcgaagtgga tcacggtgtc gatagcgtga tcgtgcagta tctcggtcat caacgcttcg
     9841 ttacgaatat cgccttcaac aaacgttgga tgtttgccgc ctaaacgctc gataacaggc
     9901 agtacgctgc gcttactgtt acagaggtta tcaagaatga tgacatcatg accgttttgc
     9961 agtaattgca cacaggtatg acttccaatg taaccgctac caccggtaac cagaactctc
    10021 ataattcgct ccattaggct tatggtatga aataaccata gcataacaaa gatgcgaaaa
    10081 gtgtgacatg gaataaatta gtggaatcgt ttacacaaga atttagccgt tttttatgcg
    10141 cgattaagtg attataaaac agagggttta tgaatgattg cgctttttat ctgaaaaaag
    10201 aagcggtttc atgcctgcat gcgtcgaacc gttggccgga gagggcgctt aggccgcctc
    10261 cggcaaggtc agcactaccg actcaatata tttttgtcag cacatagcga tagagtccac
    10321 cgtccggcac gaactcaagg cgatgggtaa tacaggcagg cgcatcttca gcgtggtgcg
    10381 aaacaaacaa caattgcgtt tcaccttcgc taatcagcac atcaacaaaa cggcggataa
    10441 gctggcgatt gagcggatca agcccctgta gtggttcatc gagaataagc aacgtcggat
    10501 gtttcaccag tgcgcggacg atcagcgcca gacgctgctg tccccaggaa agactatgga
    10561 acggagcgtc agccgtacgt ttatcaatgc cgagaatatc cagccactgc tgcaccagtt
    10621 tttgctggcg atccgaaacg gcctgataaa tgccaatcga atcaaaatag ccagaaagaa
    10681 tcacattacg cacggtagtg ctgacccggt aatccagatg caaactactg ctgacgtaac
    10741 cgatatgctt tttgatatcc cagatggttt cgccgctgcc gcgacgtcgg ccgaaaagcg
    10801 tcaaatcgtt gctgtaacct tgcggatgat cgccagtaat caggcttaat aacgtcgatt
    10861 ttcccgcacc atttggcccg acaatttgcc agtgctcgcc aggattcacc tgccagctaa
    10921 ggttattaag aatggggcga tcgttataag aaaccacgcc attattcagc acaatgcgcg
    10981 gttcgttggt gggtaaggcg tgacgtgctg aaggttcatc cggctccggc agttgcacac
    11041 cttcaagctg ttcgctatgc gccagttgcg cgacgagtgc ttgctggagc agttcctctt
    11101 tagcgcccgt ttccgctaac gtacaatccg ccagcacgcc agcaaactgg acaaattccg
    11161 ggatctcatc gaagcgattg agcaccagta ccagagtaat accggactga tgtaacgagg
    11221 cgagtcgctc agccagctgc tgacgtgagg caacatccag gccatcgaac ggctcatcaa
    11281 gaatcaacaa gtcaggctcc gacatcagcg cctgacacag cagggttttt cgcgtctcgc
    11341 cagtggaaag gtatttaaag cgtcggtcga ggagggcggt aataccgaac tgctgcgcca
    11401 gttgcatgca acgcggtgca tccttaactt catcctgaat gatctcagcc gtagtgcgtc
    11461 cggtgtcatc ttcgccaggg ccgagcatat cggtgttatt ccgctgccat tcgtcgctga
    11521 cgagcttttg caattgctcg aaggagagac gagtgatgtg ggaaaactgg ctttgccgtt
    11581 cacctttcaa aagcggaagt tcccccgcca gcgcacgggc cagcgccgac tttccacttc
    11641 cattcgaacc gacaaacgcc caactatcac ccgcgtttaa cgttagctga ggcaattgca
    11701 gcgtttttgt gtcgctaaga cgaaacgtgc cttgcaaaat ttgcaacgat gacattttat
    11761 atcccatttt gtgcagcgat tactgacagg gatacgtgtt tcataacaaa ttgtcaacac
    11821 gcttagcata gcgtggcgat aatcacgctg tcggcattaa agtaggccgt gacattctgt
    11881 ccttgctgaa gagaagtcgc ttcattaacc ggcactgtgg cgcacagtgt ttgcccgtcg
    11941 ggtagcgcca ttaatacttc gcactgctct gcgccgcgct caatatgact aataataccc
    12001 ggtaattggt tgtcggcgtt ttgtgcgacc gcttcgtcct gagtaatgcc tacccacggc
    12061 gcttttagca atatcaacac ttctttgcct tcatccagcc ccagacgtgc gccgctttgt
    12121 gcggtaattg cgactttcag gcgtgttttt ccgtcagcca gtaagacatc aacatgctgt
    12181 tgaacgtcat catgatcgcg ggcggtgatg gtgccgaacc actggttacg ggcgctggtt
    12241 tgcagtgaaa aacgtgagat cgcggccagc aggctgttca gcggcagggc gtcatcgtca
    12301 cttaacacat caaaggcttt ttgctggatt tgcgccagta agtcatagag ctgaatcagt
    12361 cgctgaccat agcgggtcag tactgcgccg ccgccacctt taccgcctgt tgcgcgctcg
    12421 accagaatat gctcacttaa ctgattcatc tcgttaatgg catcccaggc gcttttatag
    12481 ctgataccgg catctttcgc tccctggcta atggaaccgg aaagcgcaat gtgttttagt
    12541 agcgaaatgc ggcgcgggtc ggcgaataat ttttgttgga gcttaagggt gagaaggatt
    12601 tcggcctgca taacaatgtc ctggcaaaag tcttattgtg acggaaaacg aacgccacgc
    12661 aaagctgacc gcacaaaagg ggagtgcttt tctgtgctta gcggttagaa tagtctcatg
    12721 actatatctg gagttgacca tgttagagtt attaaaaagt ctggtattcg ccgtaatcat
    12781 ggtacctgtc gtgatggcca tcatcctggg tctgatttac ggtcttggtg aagtattcaa
    12841 catcttttct ggtgttggta aaaaagacca gcccggacaa aatcattgat tccctgaatg
    12901 cccgcttagt cgggcatttt ctttttctca acttcctgct tttcctgccg atattttttc
    12961 ttatctacct cacaaaggtt agtaataact gctgggaaaa ttccgagtta gtcgttatat
    13021 tgtcgcctac ataacgttac attaaggggt taccaatggc tcgtaaatgg ttgaacttgt
    13081 ttgccggggc ggcactctct ttcgctgttg ctggcaatgc actggcagat gaagggaaaa
    13141 tcacggtgtt cgccgccgca tcactgacta acgcaatgca ggacattgct acgcagtata
    13201 aaaaagagaa aggcgtggat gtggtttctt ctttcgcttc gtcatctact ctcgcccgtc
    13261 agattgaagc gggtgcgcct gcggatctgt ttatttctgc cgatcagaaa tggatggatt
    13321 atgcagttga taaaaaagcg atcgatacag ctacgcgtca gacactgctc ggcaatagcc
    13381 tggtcgttgt agcaccgaaa gccagcgagc agaaagattt catcatcgac agcaaaacta
    13441 actggacttc gctgctgaat ggcggtcgcc tcgcggttgg cgatccggaa catgtccctg
    13501 ctggcattta tgcaaaagaa gcactgcaaa aactgggcgc atgggatacg ctctccccga
    13561 aactggctcc ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc aatgaagcgc
    13621 cgttgggcat tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa gtggttgcca
    13681 ccttcccgga agattcacat aaaaaagtgg aatatccggt tgctgttgtg gaagggcata
    13741 acaatgcgac agtgaaagca ttttatgatt atctgaaggg accgcaggct gcggaaatct
    13801 ttaaacgtta cggatttaca accaagtaat gatactgacc gatccagaat ggcaggcagt
    13861 tttattaagc ctgaaagttt cttccctggc tgtgctgttt agcctgccgt ttgggatctt
    13921 ttttgcctgg ttactggtgc gttgcacgtt tccgggcaaa gctttgctcg acagcgtact
    13981 gcatctaccg ctggtgttac cgcccgtggt cgtcggttac ttattattag tttcgatggg
    14041 acggcgcgga tttatcggtg aacgtctgta tgactggttt ggtattacct tcgcctttag
    14101 ctggcgcggc gcggttctcg ctgccgccgt catgtcgttt ccgctaatgg tgcgggcaat
    14161 tcgtctggcg ctggaagggg ttgatgtcaa actggaacag gccgcaagaa cactgggggc
    14221 cgggcgctgg cgcgttttct ttactatcac gttaccgctg accttaccgg gaattattgt
    14281 tggtacggta ctggcttttg ctcgttctct cggtgaattt ggcgcaacca tcacctttgt
    14341 gtcgaacatt cctggtgaaa cgcgaaccat tccttctgcc atgtataccc tgattcagac
    14401 ccccggcggg gaaagtggcg cagcgagact gtgcattatt tctattgcgc tggcgatgat
    14461 ctccctgttg atttcagaat ggctggccag aatcagccgt gaacgggcgg ggcgctaatc
    14521 atgctggaac tgaatttttc ccagaccttg ggcaaccatt gcctgactat taatgaaacg
    14581 ctgcccgcca atggcatcac tgctatcttt ggcgtctccg gtgccggaaa aacttcgctg
    14641 attaacgcca tcagtggact gacgcgcccg caaaaaggga ggattgtcct caatggtcgg
    14701 gtactaaatg atgccgaaaa aggtatctgc ctgacgccag aaaaacgtcg cgttggctat
    14761 gtttttcagg atgcacggct gttcccgcat tacaaagtgc gtggcaattt gcgctacggc
    14821 atggcgaaaa gcatggtcga tcagttcgat aagctggtgg cgcttttagg cattgaaccg
    14881 ttgcttgacc gtttaccggg cagcctgtcc ggaggcgaaa aacagcgcgt ggcgattggt
    14941 cgggctttgc tgacggctcc ggaattgctg ttgctggatg aaccgctggc gtcactggat
    15001 attccgcgta aacgcgaact gttgccttat ctgcaacgtc tgacgcggga aatcaacatt
    15061 ccgatgttgt atgtcagcca ttcgctggat gagatcctcc atctggcaga cagagtgatg
    15121 gtactggaaa acggtcaggt gaaagccttt ggcgcgctgg aagaagtctg gggtagtagc
    15181 gtgatgaatc cgtggctgcc gaaagagcaa caaagtagca ttctgaaagt gacggtactt
    15241 gagcatcatc cgcattacgc gatgactgcg ctggcgctgg gcgatcagca tttgtgggtc
    15301 aataagctgg acgaaccgct gcaagctgcg ctacgtatcc gcattcaggc ttccgatgtt
    15361 tctctggttt tacaaccgcc gcagcaaacc agcattcgta acgtattgcg ggcaaaagtt
    15421 gttaatagtt atgacgacaa cggccaggtg gaagtggagc tggaagtcgg cggtaaaacg
    15481 ctgtgggcgc gtatcagccc gtgggccagg gatgaactgg cgatcaaacc tggcctgtgg
    15541 ctgtacgcgc aaattaaaag tgtgtcgata accgcctgat taaatcaggt ggctataaat
    15601 gaactgggca atgctgtcgg tggtgttatc accaatcaca atgttggcgc gcgcttttac
    15661 cgcgtcatcg gcgttgccca tcgccacgcc tgtaccagcg gcttccagca tactgatatc
    15721 attaaagtta tcgccgaatg ccacgacgtt ttccatcgac caaccttgcg cctcaaccca
    15781 tttcgtcaaa cgtttacctt tgctgttgcc gccgcgtgca atatcaacct gatcgtgcca
    15841 ggaccattca cactccagtc ccagttcatg ttcgacatgc ttaccaaaat gctgcaattg
    15901 cggcaggtca tcgtgcgtca gggcgaactt ccatacggcg ttaacttgtt gcgccgtttc
    15961 agccagagaa gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg tttgcgccca
    16021 gttagacgtg cgaatgacat gcccggtcgg gtgctcatag accattgcat catcgacata
    16081 catcagaccg tgaatgtggt gttcattcag catctcaatg agttgcaggg ctttattaac
    16141 gggcattggg tccgcttcca gcacggtttt tgcatgataa tcatacaaat aggtgccatt
    16201 acagcaaata gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa tagcgacgtg
    16261 atggcgacct gtgacgatga ttaattgata gcctgcttcg cgagcgcggg ccagggcttc
    16321 tatcgatgaa ggaagcaggg tctttttcgg ggtcaataag gtgccgtcta agtcgagagc
    16381 aatcacgcgt gtggtcatgg cgtattccag attaaggtta agaattttcg tctgcgcgaa
    16441 tggtacaccg ataccactat cggacaaaat tctgcgtttt aattcagcat tcaccgccaa
    16501 aagcgactaa ttttagctgt tacagtcagt tgctaaatgc aaaggagcat tcatgaagca
    16561 aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga
    16621 aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt
    16681 cagcccggac aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta
    16741 tcgtattgcc ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag
    16801 tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc
    16861 gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt
    16921 cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt
    16981 tccggcatta aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctcgt
    17041 ggcgcaggac cctgcggaag tgaccaccgt tgaaggggcc ggcccgcgtc atatggtatt
    17101 ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg
    17161 ggaactgaaa gatccgcacg gtaatatcga atgtgtccag acgctggata tgatgccgga
    17221 aaacttctcc gacacccgtt gggcggctga tattcatatc accccggatg gtcgccattt
    17281 atacgcctgc gaccgtaccg ccagcctgat taccgttttc agcgtttcgg aagatggcag
    17341 cgtgttgagt aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga
    17401 tcacagcggc aagtatctga ttgccgccgg gcaaaaatct caccacatct cggtatacga
    17461 aattgttggc gagcagggac tactgcatga aaaaggccgt tatgcggtcg ggcagggacc
    17521 aatgtgggtg gtggttaacg cacactaacc gctgatttaa ccggcgcagt ctctcctgcg
    17581 ccggtgtatt aacttatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac
    17641 aaattgtctt tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttcagctgcc
    17701 ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc
    17761 accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc
    17821 gccgaaatcc ccagtcctgc ctccactagt cctaatgacg tcgaaagatg acgcacttcg
    17881 taaaaccagt ccagcttcca gggcttgtcg gctagctgtt gttctatcaa caatcggttg
    17941 ccgctggagg aacgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc
    18001 tgctttttgg ccaatggatg gtcacgccga caggccagca cgaatggctc gttgaccagt
    18061 ggggtaaaat caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg
    18121 ttacacagca ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata
    18181 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
    18241 ggaatacacg ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
    18301 agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaacct tttccctgct
    18361 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
    18421 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
    18481 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
    18541 attattactc cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac
    18601 tgcatttagc caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta
    18661 gagatgcttt gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
    18721 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttct
    18781 tgttagcgga acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
    18841 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatccg ctgaccagta
    18901 aagtcgccat tattagccgt tccagcgatc cgcgtgctga tgtcgattat ctgtttgctc
    18961 aagttattgt ccatgagcaa cgtgtcgata ccacgcctaa ttgtggcaat atgctgtctg
    19021 gtgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcacca gttacccgcg
    19081 tacgtatccg caacgtcaat actggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
    19141 gtgttgtcga gtacgagggt agcgccagaa ttgacggcgt accgggtact gccgcaccgg
    19201 ttgcgctcac tttcctgaat gccgccggaa ccaaaaccgg aaaagtcttc ccgactgata
    19261 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg
    19321 tcattattcc ggctgaatat ctgggaaaaa caggttatga attaccggcg gaactggatg
    19381 ccgacaaagc attattagca cgcattgaat ctatccgtct acaagcgggt aaagcaatgg
    19441 gcttaggtga tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga
    19501 aaggcggggc aattaatgta cgttatttta tgccgcattc ttgccatcgc gcgctggcga
    19561 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa
    19621 tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt gcgctcgacg
    19681 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacga
    19741 ccagaaaaat attttccggt gaagtttatc ttccctgaaa aaattcgttg tcaggataag
    19801 gacaatcaat aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata
    19861 agaaatcgtt atggaagcta attctgatat tagcgatccc atgtattatt ggttttatgc
    19921 cagctccggc aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca
    19981 ttgtgggtct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg
    20041 cctcaatggt ggtggtcggt aacttatccg acggtgcgtt taagaccacc gccgtattaa
    20101 gcggttactc ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat
    20161 ttgtgaccac cggtttaggt aaacgtattg cctatctgct gattggtaaa atcggtaaca
    20221 ccacgctggg tctgggttac gttacggtat tcctcgatct ggtactggct ccggcaacac
    20281 cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc gtggcggtgg
    20341 ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca
    20401 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
    20461 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcgc
    20521 tggccgccgg attgccgggc atcattatgc tgctggtcac cccgctggta atttacacca
    20581 tgtatccacc agaaattaaa aaggtggata acaaaaccat cgctaaagcg ggccttgccg
    20641 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctac
    20701 tgggctggat tttcagtaag tctctggggg ttgatgaatc cactgtggca atcgttgtta
    20761 tggcgaccat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg
    20821 gctggaatac cttaatctgg tacggcggta ttatcggatt aagctcctta ttatcgaaag
    20881 ttaaattctt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
    20941 gtaacgttgc tttcttcgtt attattttcc tcagcattat cgtgcgttat ttcttcgctt
    21001 ccggtagtgc ctatatcgtt gctatgttac cggtatttgc catgctggcg aacgtctccg
    21061 gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg
    21121 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttat aacgatatta
    21181 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcctggtg catatcaccc
    21241 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata ttctcgtcat
    21301 acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc
    21361 tcctggggat tcactcgctt gtcgccttcc tgcaactcaa attatttaga gtatatccat
    21421 ttattatctt tctgcgcact tcacggtgcg cagatatctg gagcatttga tgatcaagtt
    21481 atctgaaaaa ggcgtgtttc tcgccagtaa taacgaaata attgctgaag aacatttcac
    21541 cggcgaaatt
//
LOCUS       DAFAVF010000065        21523 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFAVF010000065 DAFAVF010000000
VERSION     DAFAVF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.065"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..2150)
                     /locus_tag="LGK42_002450"
     CDS             complement(<1..2150)
                     /locus_tag="LGK42_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBK2658690.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGILPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDN"
     gene            complement(2247..2831)
                     /gene="traX"
                     /locus_tag="LGK42_002451"
     CDS             complement(2247..2831)
                     /gene="traX"
                     /locus_tag="LGK42_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBK2658691.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(2860..4062)
                     /gene="traW"
                     /locus_tag="LGK42_002452"
     CDS             complement(2860..4062)
                     /gene="traW"
                     /locus_tag="LGK42_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBK2658692.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(4029..4643)
                     /gene="traV"
                     /locus_tag="LGK42_002453"
     CDS             complement(4029..4643)
                     /gene="traV"
                     /locus_tag="LGK42_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBK2658693.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(4643..7687)
                     /gene="traU"
                     /locus_tag="LGK42_002454"
     CDS             complement(4643..7687)
                     /gene="traU"
                     /locus_tag="LGK42_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBK2658694.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            complement(7777..8577)
                     /gene="traT"
                     /locus_tag="LGK42_002455"
     CDS             complement(7777..8577)
                     /gene="traT"
                     /locus_tag="LGK42_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBK2658695.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(8561..8749)
                     /gene="traS"
                     /locus_tag="LGK42_002456"
     CDS             complement(8561..8749)
                     /gene="traS"
                     /locus_tag="LGK42_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBK2658696.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(8813..9217)
                     /gene="traR"
                     /locus_tag="LGK42_002457"
     CDS             complement(8813..9217)
                     /gene="traR"
                     /locus_tag="LGK42_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBK2658697.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISTISIG"
     gene            complement(9268..9795)
                     /gene="traQ"
                     /locus_tag="LGK42_002458"
     CDS             complement(9268..9795)
                     /gene="traQ"
                     /locus_tag="LGK42_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBK2658698.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(9795..10499)
                     /gene="traP"
                     /locus_tag="LGK42_002459"
     CDS             complement(9795..10499)
                     /gene="traP"
                     /locus_tag="LGK42_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBK2658699.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(10499..11788)
                     /gene="traO"
                     /locus_tag="LGK42_002460"
     CDS             complement(10499..11788)
                     /gene="traO"
                     /locus_tag="LGK42_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBK2658700.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(11791..12774)
                     /gene="traN"
                     /locus_tag="LGK42_002461"
     CDS             complement(11791..12774)
                     /gene="traN"
                     /locus_tag="LGK42_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBK2658701.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(12785..13477)
                     /locus_tag="LGK42_002462"
     CDS             complement(12785..13477)
                     /locus_tag="LGK42_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBK2658702.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(13474..13821)
                     /locus_tag="LGK42_002463"
     CDS             complement(13474..13821)
                     /locus_tag="LGK42_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK2658703.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(13839..17606)
                     /locus_tag="LGK42_002464"
     CDS             complement(13839..17606)
                     /locus_tag="LGK42_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK2658704.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(17695..18246)
                     /locus_tag="LGK42_002465"
     CDS             complement(17695..18246)
                     /locus_tag="LGK42_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBK2658705.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            complement(18261..18551)
                     /locus_tag="LGK42_002466"
     CDS             complement(18261..18551)
                     /locus_tag="LGK42_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK2658706.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(18548..19696)
                     /gene="traJ"
                     /locus_tag="LGK42_002467"
     CDS             complement(18548..19696)
                     /gene="traJ"
                     /locus_tag="LGK42_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBK2658707.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(19693..20511)
                     /gene="traI"
                     /locus_tag="LGK42_002468"
     CDS             complement(19693..20511)
                     /gene="traI"
                     /locus_tag="LGK42_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBK2658708.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(20508..20966)
                     /gene="traH"
                     /locus_tag="LGK42_002469"
     CDS             complement(20508..20966)
                     /gene="traH"
                     /locus_tag="LGK42_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBK2658709.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
BASE COUNT         4815 a         5364 c         5616 g         5728 t
ORIGIN      
        1 ttattatcat cctttgtgac attttttagt ctgtcctctc taactccagg gctgtgtcta
       61 atatttctat tagaacctgc aaaaatatct tttactgatg aggtgaggtt gccaagagta
      121 tttacaccat ctctaccacc caagaaattg ataacatggt caggaagata tgcttgtaaa
      181 gcaaatattg cagctacaga cgttgtacac atacgggcgt aaattagcag gaacccggca
      241 aggctgaata tccctgttaa tgattttacc tgtacgttaa ctagtgctgc gccaaacagt
      301 gagtttaata ttgtgccgac ggcaatgatg gcgacagagg caaaaaagaa accgaatacc
      361 atgagaggtg gtcttatcat gctgtcaatt aagtatatat atccgtaagc tgctcgactt
      421 cctctgtcct ctgatgttcc tagatgtgtt gctgcccaca gaggcccggc agtgcatcct
      481 attaatacgc ttactatcca gttcccaatg cctgtcatcc agaaaatgaa tggtataaat
      541 ggtaggtaaa ttgataagga aaaacccgca caaaacaaca ggaacaacaa gaaatatatt
      601 gggggagctg cagcatccag cagagcaaca aatcctttag gtaatccaga gaacaaatta
      661 acagcctttc cgaaaataga gttatcaccc attgtcgcga gaactctgac agttgtatat
      721 cctgtccaga gaatttctgt tgtacccaat gtataatcgc cgatgttttt catcttaatt
      781 aatgggttaa cttggttgct ggttgttcct gttcctgacc attcagtagc agcttggttt
      841 gtccatttta atatggttgg cgctatactt ttcatgacca cactttgtgg gtctgtgcta
      901 ttagctgcat tactttcatc ttgagttgtt atcgttccta tcgttggagt atatgtggag
      961 ttctgtaatt gagtcttgta tgcccccata acagcactaa ataaatcagt atcgccaact
     1021 tccccaagtg atgacattga agttacagct ggagctctgt cagccagctc tgccagacgc
     1081 tggttagcag tggcaaaggt ctgataccac gcgccaagcg tcacccatcc ataggttgtg
     1141 agataagact tgagagcttc tttcctggat tgttctccgt tatcaatagg tagcgatttt
     1201 tgtacagcgc gttcatactc gtcggctgct ctctgaatcc gtgtctcgat atccggcaat
     1261 attccgttgc cagaatttct tttttccaga aatgttgtca caaactcact ggcagcgttg
     1321 tccatgtcgc taatcatatt atccatggct gcacgttgtg ctgagataac accgctgtat
     1381 tcgtttttac tgaagggatt aaagaatttt ccaatcgtcg attgatctgt tgttccgttt
     1441 ccttctactg acaggctggc tgaaccacag ataccgctgc cattggatac cgtgacagtg
     1501 taattaccac tggcggtttt agctgattct gtcatcagtg aagtcgagga tttgaccgtc
     1561 tgattaaaat catttagtcc tgcgttaacc gcatacttac acagttccat ttcaaaaatg
     1621 ccacgcgctg ctgttcgtgt ggatgcctga actggctgta cagtcattga ataaccattt
     1681 gcgatattat cagcggcaag ttgcaccatc acattggcgg acccaacccc cattatcgat
     1741 gcgccccata acataatgag ctgagcgata ctccagccat ttccggttgg aacgatcatc
     1801 agaaaacctg caacgacact cagtgtgccg acgatatcgc gtccggtgct gaatacctgt
     1861 ccctgatgcc ccgaacgcac aacatgacgg ataccaatga acataaacca gacaacggca
     1921 agtacggcga tgatactgtt gaacgccccg aacatgcttc cgatcagagt gggggcgctg
     1981 gttgacagtg gattggtgac tacatcaccg aatatcgtga ccagcgcctg gcgagacaga
     2041 tcgtcaggat tggttgcggc agacacaata tcctgatagg tcactgaagc cattgccggc
     2101 agtgaggata tagcaaggcc tgcacagagt gcgcgaagta aaattttcac agtaactccg
     2161 gaatagaatt gcgtatgggc aaaggccaca caggatttaa ttaaggggga tggcgacatg
     2221 ccagtggtac tggcatctgg acgtgatcag tattgtttac tggttacggc aattaatgtg
     2281 gcattgcgcc agccattgcg gtcgttcaga aaatcacgga aagtcccctg ctcgggttcg
     2341 ctgacttttc gttcatgcaa ctgccagagg cggtatgtaa caatcagtgc tctggaagca
     2401 caaagagcga cgcctgacag tatcaacaca agagtgctga tggcgcgtac cagagtcgta
     2461 agaggtaatg aagaggcggc aaagagcata ccactgagaa acaaagcgat ggccagcaga
     2521 ctccagcaac agaatcgcca tactgttctg cgccggcgga aacgacgatc cagatcgtct
     2581 actgacatat ggctggcctg cagagcttct tcccacgtga ggtggagttg cgccttttta
     2641 ttatttacct gacgaaatct gtccagcctg cgtttttgtt gttccagcat atgctgtaca
     2701 gaattcgtta tcaggcgagt ctccatcaga ggaacaaaaa tgttaataat cccccgggtg
     2761 gctatgcggg ttatggatgg tgattttttg acgtttttcg tctggtctga attttccggt
     2821 aacttattca ttttttcagt cctgttctga cgtcagcttt caacgtgcgt ttgcctgatt
     2881 gattgttttc tcaaggctac cgagttgttt ttcataatac tggcgggcgg tcagagccag
     2941 ttgctgtcca gagatgacat taccctggcg gatctgttct ttcaggtcgt tcagttgcca
     3001 gttcatttgc gcagtgattc ttaccagttc acggagcagg ttatctccct gcatttcctg
     3061 taaatccacc aggtatgctg tgttggcgta gcgccggcca gcctcaaaag cttcaaactc
     3121 acgggtactc atatatcccc gtgcttttgc ttctggtgaa gccacctggt cgaagtaagc
     3181 ggccgctgag tctgactgca gtgtttcagc cagtgcttta cgtgtgcttt cattaggcgt
     3241 gctgtcagca atcagcgtca gttggggatt cgacgcagaa tcaatgatgc cgttgtattc
     3301 gttctggagt ccgacgtagg ttcgcccact ctgggtattc acttctcctt tacccagagc
     3361 acgaccggca gaggggcggg aagtgttttt catgtaagcc gtcgctgcat ctatttgctt
     3421 ctgatcccat gtcagtgctg caggtgtgtc cgctgtacct gcgccgtggt aaatcgagcg
     3481 tacctgactg tctccacctg gtatttcgcc cacagaagga caaacggcgg ttcctccaaa
     3541 acgtgcatat tctgcttctg tacagtaact ggcgtgtatg gccgcgccat cataagcttc
     3601 ccgcacggga gagtttgctg cgctggccag acggtcgcgg atgctacggt tgctcacacc
     3661 gccaccttta ctgagttttg aggctgcgga tgctgaggca cttttgcttt ctgttgcaat
     3721 ccctgatgcg gattcactgc agatggagtc tggcacggtg aagcttttcc gtgcctcctc
     3781 gaggcgctga gtttcattac tgaaaatcat ctgctgacgt gtcgcctgtc cgttctgggc
     3841 aatgacactt gccagctttt cagagttctg attgatagcg gtacccgtgg ctgtctgggt
     3901 actcagtatg ttgccaagga catctttgat ggcttccatt ctggggatga cctgctcttc
     3961 aacaggccgg ctggcgacga cggtgactga ataagcatga gcctggcatg ccgttccact
     4021 cagggtggct ataagggctg cgagtaatgt ttttctttgc attttattgt ctcctgctcg
     4081 ggatggagca caaggtcagg aaggctgtcg tattttctgt tttcaggtag tggggccagt
     4141 gggaaacact gaaatcgcct ggtgatataa cctttcctgt tccggttatc gatgatgctg
     4201 aaagaacgac gtctgacgcg ccgtaaggtg gaagggtcag tatccataac aggccagtca
     4261 ccggcactca gtgccggcgt tccgggaacg gatggccata accaggtatc atcggacatc
     4321 agcgaagcca gcggtgaagg gagtatccac tggatgacat gctgaacgtc gaatggtgct
     4381 tcatccggga cagaaacctg ccatattgcc gttgttatca gctcatctgt gctggacggc
     4441 aacaacaacc gcatgcctgt aacctttccc tgtgttctgg catccgaggc gtatatcact
     4501 ctgggattgg gagccggatg taacctgaag acacgtgacg gcagagtgaa tgagaaactg
     4561 tcacctttta cctcaagttt tttaagaaat acctgccctc tggcaggtct ccggcagcgt
     4621 ggggtgatat gaattgtaca catcagagat tgtatccctg ttttcggatg agctccgagg
     4681 ccagttcttc aataacattg tcactgttat gttggcccgc ccggtgctca attaatgacg
     4741 ttgcagaacc tctggggaag ttttcggcca gaatcttacg tgctcgaact gaacctaact
     4801 tatttgtcag tgttttacgc agggcactgt cttttggcga tgagttcagc gcccatagct
     4861 ccagtggacc gacggtaaat ttgagtattc tcgcaagcgt gccacttttt accctgaata
     4921 ctcccagaac aggaacacca ctaccatctg gtgccgggcc ttccggcatt ttcaggaagc
     4981 gtttcagcat aaattcagga acgttgaagt tatccctcag tacggggata tcttcgggtt
     5041 tgtagcgcag taaccagaga gtatttgctg acttaagaac gctttccggg tagtccctca
     5101 gatactgcgt actcagaacc gtgcggatag caaacttgcg ttgctcacgc tcctgggtat
     5161 ccagattttc ccagatgaag tcaatgcccc gggcgttatg cagttcatca taaacttttg
     5221 tcttaacctc ctgatcaagt tggttaatac gtttaagagc gatttcatgg tattcccgtg
     5281 gaagctgctt taacacttca tccctgtatt gtggcagggt gaagtcacca ccggcgatct
     5341 gacctgcaag caggtacatg atgccggttt taagcctgcc tgcaggcgtt ttgtcaccgg
     5401 caacattatt cagatcgaca gcgatgaccc gggtttccgg gttgatcata aaccgggtat
     5461 acccggatat catggggtag tcgttatgtc cctgctccag ggcgcggcgg atgtagtcca
     5521 gcagaagctc gtttgaacca tcacgctgaa cagtaccgaa aacatccctg agactggtat
     5581 ggcccagcat cgaggacact tcgggaagct ggggcattgc ctgataatgt gcccgctgag
     5641 ccgccatgat atacccggca ttgtggagca tatcccggac ttcaaaccaa gttgatctgg
     5701 cccaccaggc tgcatcatgt ttttcataca gtcccgagtc ctgcagtgct gaatccacca
     5761 gttcttccgt tgaggcgcga taaagtcgcg gattattttc accgtactct ttaaaagcca
     5821 gctcgataag ctggttgata atttgtcggg tgtctccggg gttacagggt tgaccggtac
     5881 cggtatcgac acagagagca cacagcaccg atgacatgaa attcttttcc ggcgtaatag
     5941 gctttttagc gccgtacatc acgtcaaaaa ggttacgggt gtattccgga tcattgctca
     6001 gaataatacc gacagcttcg tcttttcttt ccggaggaag actgtcgcga ataagctgaa
     6061 ccagtccctg agcactgtat cccttatcga tataggcaat gaagggcagg tttttctgag
     6121 cagatgatat ctgtatttcc gacaatgtat taatcagaac tgatttaccc agtcccggat
     6181 cgcctggtgc gagttctgta tgtttatttt gttgggatga cgccagacca acctcaattg
     6241 cggaaccgtc ttctgtatgt aacatcagat tccctttgcc tcgccagaca gagcctgccc
     6301 tgttaagcgg gaaaagcgag atggcatgtg acaacggggg atacagggga accgggccgg
     6361 agcctccgga tgccgcgaga atggtattca cccaggcgcg tcggggatcg ccaaatgttg
     6421 tcgtggtgcc acatacgccc cagccttcaa tggctgattt cagaatggcc tggtttcgtg
     6481 tgcatatttc acgtgttttt ccccacgtgg aggccatgat ggtcattatg cagacaggtt
     6541 ctttttcatc cgttgccgcc agggccataa cggattcata cattggacga acggcagaaa
     6601 tgaaactgct gtaagtcaga agcgttttct tcagattaag agccttcatc ccgccgggca
     6661 tcagatccat tctgatgcgc caggggactg cgcgggggac ggcacggacc agttcgttaa
     6721 acgtctgcag attttgcgga ggtaaagtaa tcgataccat tccgtgccag agtccgcccg
     6781 cctgaaccag gttgccctgt gtggtcacct gagtgttgaa taactgcaaa tgcaatgaag
     6841 gagcatgtag tacggattca tcgtcagtcc agcgatatcc cgctggctgg gcatcttcag
     6901 gcaggtgggg ttgccagtta cgcggtgtgc tgtgccgttc tgtctgacga cgtatttcac
     6961 gcccgacctc gtgtatatcc agtagccgga gaatcaggcc atcagatgaa tgtctcagtg
     7021 cctgttccac attatcaaga aaagcttcgt ggcggatttt cagtgcagat aaggtccact
     7081 gccaggggga ctgggcaaag cgtgctttcg gtacccgttc agccagtctt ctgacaagct
     7141 catcatgggc ggtgcgatcg ctgttgctga taaggtcggg tccactccag atcgccagcc
     7201 agcaacgctc ccggaccagc catggggaca gtgttgtgac tttctcatcg accacatcct
     7261 gtaactggat acctgtatta gccagtgagc gcttttgtgg ggcgaccatg tcttcgattt
     7321 cttctttccc catgtcagga tcacgctcaa aaacgaagct gattttatga ccgctgtttt
     7381 tatatgccgt attcaggctg tcactcatgc tggtgatcag cgattccagt gaacctgggc
     7441 cagtctggtc ggatgcttca tccatttccc tgaaagcacc gctgacttca aagacagaga
     7501 gatattctcc acgctttgtg gctgcaatat agggcgaatt cagccatggg cgacgttcac
     7561 gatcctgtct gtccagacct attacagtac gtaaatcgca atactcaaca aaactgcttg
     7621 cgacagagaa acgggatagc gtgttcagac agtcttcgat agtgaagatt aaacggttta
     7681 tgttcatagg tgagggaact ccggacttac aggagggagg tttgtgcagt ttgatttttt
     7741 taatgccacc ccgcgctgga gtggcatgaa gaaaaatcaa tcaacgtatt cttttttgat
     7801 gaatgaatct ggtgtcagaa tcagggcaat cccgttcatt ttttcacgca ggggcgaacg
     7861 accggaaggt ggatagcgac gaaaaacgtc agcgaaatcg gccgggcgga aactgcggaa
     7921 tttatcctca accgccagtc tgttttgcat cagtgcagag agcgcttccc gtccccgccg
     7981 gacccggaac ttatcattac tgaaaacttc ctgtaatgaa cgacggttaa gggccacgac
     8041 cgtggatgga tgaaggtcgg gtagccacac cataacgccc tgtgctgcgg cataaccatt
     8101 cagtgaatgc caggcgtggc agaaagcaca taagctgtaa ggcgggccgt cttcatgaaa
     8161 cagttcctgc tctttctctg acaacgactc tgtactggtg atttcagctt tctgtacatg
     8221 gcggagtgaa agacgtctgg cagacagacg ggcctgctca agcgcccgtt cagcggcttt
     8281 tactgccgtg ttgtactttt ccttttcgcc ggttgcgact ggttcaccac gcttttttaa
     8341 cttatccaga tgttctgtgg ccttattaag acgaactcgt gtgtcactga ttgtgtcggt
     8401 aagctgtctg agtggccatg agtcatccag tctgaaacgg tgcctgacgt tttgccggca
     8461 gcagatacat cgaccgggcg ttgttaattc cggttccggt aacagtggct cctgtgtatg
     8521 aaccacatca atttctggaa agggatacaa ccacaagtct ttaatgctgt gttgcatgag
     8581 caggctccgt actggtgtaa cgtttcacgg cttccagaat gatgtttgcg atttcatcgg
     8641 aacagcattc tgcaaaaatg aattcctgat cgtcccgact gtcgtgggca atcacattgc
     8701 cgttttctgt tcgtcgtaac gttatgtgct ccgggctcca ggcagacata tgtgcctcct
     8761 tgtattgccg aacaggaagg gcaccggata aatgccagtg ccctggacaa gattagccaa
     8821 tggaaattgt ggaaatcccc agctgtttct ggctgcgact catcatttca ggtccggcgg
     8881 caagtagcgc cccaatgacg atagatagaa tgcaaccagc cagtcccatc cccccttgtt
     8941 tcccaacttt tttcagccca atcaggcccc cgagaaacgc aattacgccg atgaactgcg
     9001 caatagtcag tgatgatgac tgtgctgttt tggcaccttg ttcgacattg cgaaccatat
     9061 cggcaagatc tccatcggca agaacggctt tgggcagcaa catcatggga atgagcagca
     9121 ggaacttagt cagtttgctc cggcatgaca ttacaaacag ttgtgcgtgg ataaatgcgg
     9181 cgtacagacg ggccgctacg acacgaatac gtgacatagt ttctccttaa atggatatat
     9241 cagtggttta tatgaaggtg aaaaaaatca gaagacgatt ccaatgcttg ccagcagggc
     9301 attgaggaca cgatcgataa agaccagcag gacgccggca ataaatttga cgtttccctt
     9361 actgacggat tcactggcac tcagtgcggt atgtccatcg agtccggcct tgcgccagat
     9421 ggatatcccg ttcatgacaa atcccacacc ggccagcttg gccagcgaca gaactgcatt
     9481 tgctgcgcca gcagcagcgc caaacgtctg aggttgcaca tatgcaatcg ggccaaatga
     9541 gtcagccctg aatcccattt gtacgcctcc ggcgttaatc attgccggga gtgatacgag
     9601 catgcagctg gttaacatcc ccgcaataaa ttttccggga gacattttta ccggaccggc
     9661 tgaacgggcg cgggcaaaca gggtaatcat gctgatcacc ccgatgaatt ctccccaggt
     9721 aaacaggaaa tccattccgg cactgaaaat actgctggca aagttctcaa tggcggtcag
     9781 ggcatccatg ttcatcagcg taacgtccct gcactggtga tgatggtccg ggtagtcgga
     9841 tcaatccgct ggatcaccac attgcccagc gtctgacctt ctctgacagc ccaggtacta
     9901 ccctgccagc gaatccaggc cataccagtt tccatcgaca tgattttcat tccgttaagt
     9961 acagacgacg tttttttctt tgctggatgc ggtgtggaag aggattcagt gacgcttttc
    10021 tgtgtcatta ttccgttcag gcggctccgg atatcatcca gctgacggcg tgtttcctgt
    10081 gcgtattctt catccttttt tatagcttcg cccagcttgt taatgctgtc gtgaagcata
    10141 ttcagtgttg cctgaatttt ttcatcgcgg ttatccagct ctgtttttac atcccgggca
    10201 agctggctca tatcttctcc tgtccggaca gtcatagtct cagtggactg aacggaaggt
    10261 gatgattctt gtgtggtttg ccaggtctgt acgccagcat ccgcatcatt aaacggaggc
    10321 atgcctgttt ccgatggcgc tctcaggaac agataccata tggctgcgag caacacgact
    10381 gcacatagcc cccatgttgc tacagatatc ccccagacgg aacgtttcca gaagggggcg
    10441 ggtttctctt cgggttcacc gaagctgccg gtttcatcta tttctgtttc aggtttcatt
    10501 atggtgtctc cggttgttca tcataccgac gttgcatcac agcttttctt tgctccatgg
    10561 ctgtctgcat tcgtgcctgc gagcgttgtt ccgcctgtga tgtacgatta actgaagggg
    10621 aaatgccact ggaaagggaa gatgacctgg cgtcgtttgt cattaccggg tcaacaaaca
    10681 gaattgatac gacttcatgc tgatacactt ctacctgttt atagggctca ctttctgcct
    10741 ggcgggttaa tatctgactt cctctttccg ccataccacc ggcaataacg cccgcaacag
    10801 cttcaccact gggcattccc acgcgtccgg taaccgtgcc atagttaccc tgaataacct
    10861 gcgtgttggc atctttataa agtgtgccaa tactgcccac acctcctaac actgacggca
    10921 gaatgatatg tttaaaccag cgtgtattca cattgctggc cacggatgac atcaggttgt
    10981 cttcccgttg agcatatgca ttgaccttaa ggtccatgcc gttccagctc atccggtcga
    11041 aatggatctc cacgccgtta cccaccagtt tgactcccgg actgaacagc gttgcccctg
    11101 cccaggggcc agcgggaatg gttgcgataa ccttggatga cgggttatca gaatccactg
    11161 cagtatcaat atatgccggt atacgggtca gggcaggtat cagttgtatc cctttttctg
    11221 ctacaggcgt ggacaggctg gcattctgta atgcgaattg ctgcgtacca ttctgccctg
    11281 ttgtttctgc cggagattta ttccacatag tcgttgctat ggttggtgta ctaccggcag
    11341 gatgctgatc ttttattctt acaatcagct tttgcagccg ttccatacgc ttttcactgg
    11401 ctgctcgatc ctcctgtggt gtgccggaag cctggcgata agcatagttt tccggttttg
    11461 ctgcaggctg ttgttgcttt tccggagtgt ccggaatatc aagcccctgc ggaagactgg
    11521 caatgaacgt ctgattattg cgggctgccg ccgcagcccc tcgttcatta tccgctctta
    11581 acagttcacg atagtgaggc gtttcggtaa cgctgcgcgt accgccactt gcagcactgt
    11641 ttatgttcac ggctgactgc atatcgctgt tactccctga tagtttggaa aacgcgatat
    11701 agctgccgcc aaataagatg atgctgctca gcccaagcag ggcagccagt ttctttccgg
    11761 atttaccggc atcctgttca gcactcatga ttactccagt tctggtgtca cgtgaacgga
    11821 ctggccattc acagagaaag tcagtagtgg ggtgacggga agcttccaga gatgagttcc
    11881 gtcggcagag gacaaggtct gttcaaattc atcgcgcaat atcgcccggg aacgaaccag
    11941 aaggtcatcg ccgtgttgcc agatcgttgt atccgggaca ttacctgtga atttcagcct
    12001 cttaactgaa gggtcccggg gaggaatacc atccagaaat gcctgtaatg ttgcgtcatg
    12061 cagggcgatt ttatccgtcg ggatggagac aaccgggctg gttggtccct gccgtggtat
    12121 tcgcagatcc agacgactgt ccatttcctg actggatgac ggcgtatctg tttcgccact
    12181 ggttacgttc aggatgaccg gtaccgacag acctttcagg tagacagaaa tgtttccgga
    12241 tgcccatggc cgcaatggtg tgattgtcac catgttctcg ttgtactgca catcaaagag
    12301 ttttggtgcc gcgttataag gaggatcgga ttgtggccat ggtgaaccgt tgatatcggt
    12361 aaatgtcact acgctcaggt tgttcatggc tgtgcgaaca agaggaaggc tggcaccggg
    12421 ggacagatta actgtcagag agctgatacg gggtacaaca ctggtgatgg gcgcattgat
    12481 ggcccgctcg ttatccgcca tgagactgcg tagtgagcga atctcatcgg cggagagctc
    12541 agaatcaagt cttgccgcat cctgtaccag cgggctggcc tgcccggggg caggcagctc
    12601 accttttacc agtgttgccg gaacacctcc ggtgttttgg gaggcgttct gaacgtgatc
    12661 gctggcagaa tttgtcgttt gtggtgtgcg tgcgttctgc catcctgcgt tatctgcaga
    12721 cgtggcggcg ctgcacacca ggagcagtgt gctcagtaaa taacgtgatt gcatattact
    12781 tatctcagga gttcggtccg gcgttacggg agatcatctg ccggatttcc ataccggatg
    12841 gtttcagtct ggggtcaaca cgctggatgg tgatttcgaa aacaaaagac tgttctggct
    12901 tgctggttgt ctggccgacc agacgcatac ggacaggata ctgaaatgtc cagaaccata
    12961 cgccgtcgga catttgtccc tgtcgtacca gaacgccagc gcctgtcgtg gcggtcagat
    13021 tcatcttctc tttcttgatc gtctccagaa tgttagatgc ctgtaatgca ttcacatatc
    13081 cggcaaaacc ttcgtcagaa aaacgcggag acagagagga aatctgtgaa cgatagtgca
    13141 caaaatccag gttgaatgcg gtcgccagtg cctggctgcc aaaggccatt gcgtcattct
    13201 gactccatgc tggctgattt gttggtgcca gcctgaccag acgaccattg tctgtagcga
    13261 aatattcgcg ctccacattc gttgagtgcc agtactgaat ggcgttacca gtaatactga
    13321 taagaagaca tgtgcccgta cataaaagcg cttttatcag cgcagggaca ttatttgccc
    13381 gctgttctga ctgcttcata gccctgattg tgctcttcag tatttccggg tcaggtgccg
    13441 gatgtgatgg cgaggatggc tcggtatttt cggtcatagg ttgtagtcct tccagggatc
    13501 aatattcccc ggcagctttt cattcacctt gtccacgacc gcgtcgcaga cctggttttc
    13561 cagctgcgac agaatgtcgt caaggctggg gaactggggg aggtttatgc tgattttttt
    13621 gattcgtgac agacaggact gcagggatga ggaaacatcg ttttcacgtt gtgaccatgc
    13681 atccgcagct gcacgtgctc gctcatatcc tgtattacta ccggcctgtg ccgcgctacc
    13741 cgcacgacag gttgcagcac ttgccgtgga tgaaatcagg ataaggccga gcacggcaac
    13801 cagagactta tgcatgttca tatggcttcc ttaccggctc atcggccggg gctgtgggag
    13861 agatcctgat gcagagtttt ttcctgcctg tcctccggct tttggctgtt ggaccactgc
    13921 tgccatgact gctcgaagcc caggtttcgg tacaacgcat caggttgttt ttccggcgtg
    13981 tagacagcag caaaaagcgg ttcttcccgg gttttcagat gaaactgcat aagctccggt
    14041 gcggggcgat tcgtgctgtc agcatcgtgg tttaccgctt caccataacc tgcaaagcgt
    14101 aaacctttgt cggtcagcgc attgataacc aactgactgt tgtcggccga tggggtggcc
    14161 agtgccggat acaatgtgcc gttcagccgg accacaccct gcagatgttc atcggcactc
    14221 tggacaagta acaggttgag ttcacgcgta tccttatcca ctgatcccag aacaggtgtc
    14281 agcttgtcag gggctgcgct ggttatttct gttacctgat tcgcgttctg tggcgcggag
    14341 accggatgtc ggtcttcacc gataaacaga tgtgagccga cgttttctgg catacgtaac
    14401 gctttcatct cttcgggcca cccctggctg atttgcgcag ccactttcat aaaggtttcg
    14461 accggaatcg cctgaatacg ctgaccttta tccggattat catgtgatac cgagatcccg
    14521 ttggtaactg actccatgga ccacttattc cggaaacgtg ctttttgctc tcccgggagg
    14581 atgaattgtt cgcggttctg aagtgtcagt gtcaccacgt cacctttctg atggtttttg
    14641 accagacttt ccagttgttc cccccagtag agtttgctgc cactgcggtt tttaagctca
    14701 acaaagaaac tctctccttt gccgggtgca tattcagcac gaccaaaatt gtgcagtacg
    14761 gcagtcacgc tctctcccgg gcgaagtttt cctggtggtt cttcgtctgg tgccacatct
    14821 gccgttttgc ctggttcagc cggtgatggt gcagaggcgg tgctgacggt tgatgcttca
    14881 ggggcaacag gggacgccgg ggattgtgtg gcttcttttt cctgaacggg gtcagggaca
    14941 gccggttgtt ctggggtatt ccggttaagt tccggtgttg gctgatgggt gacaactgca
    15001 tccttactga cagccatttc cttgcgtaat ttttctagtt cagcccgttg atctggcaat
    15061 ttcatccgga catcgatgtt gtgctcgata atcaactgca ttgctttttg cttaaaggca
    15121 tcactgcccg tcagctctat gacgccccca taaaatttgg tggccacagc aagtgcggcc
    15181 agaacgctgc gatcgtcatt gctggcaccg ttgaccattt ccagccggtt tacccggtca
    15241 atgaaggcag gtttgccatc cagtttgtac aggacggttc tgtctgctcg tttttcccag
    15301 tcaagctctt taagaatttc gtccatgttg agcgatatcg gtgcatcaga acgttccggg
    15361 gcgtagacaa tactgtcagg tatcggtgga gtcgtcgtgg tttccgctga gggcgtggga
    15421 tcactgttct catgtgcctg tgaatccgtt tttttacgcg taaaaaagcc acgcgctttg
    15481 tcgagaaacg atgaagtatt atcagaaggt gatgtgctct gaacaggttg ctgtggagtg
    15541 ggttcatccc ggacagccgg ttctgcagta tctgttgtgg cctgaagttc tgactcatct
    15601 gcctgaacgt ggtctgtgtc cggttgcgac ggcatatcac tgttttcctg cagatgctca
    15661 ggattttcat tgttcatcag ctcctgatat gcgctgtagt tttcgtattc ttcggggcca
    15721 tctgactcag gaaggaacgg ctctggctga aggggaatgt cttcagttac atggttgacc
    15781 gcctcttccc ggggagtatc cggggatatt ccggtgcttt gtttgtcatc aggctccgtc
    15841 atcacggtca tttcctcttt gactgcatca ggggaaggtg atggctcggc tgattcattc
    15901 atggtttcag atgagggact gtcttcaggg gatgcggggg cggcagcagg ggccggtatt
    15961 tcaggtgaag tgccggattc ctgttgtgac tcgttttttt ccggttgtga cagttcggct
    16021 tcaaattcac gccagtcttt atctgtggcc accgagacaa ctttttccgg aggttccgcc
    16081 ggatgagcgg gttcatcagg ggcagatgcc gttgtacttt ccggggacgc cggtgattct
    16141 gcaggggagt cctgaactac agagctttcc gtttgcgcct gaacgttatc agcgtgccgc
    16201 tcatctttgc cgttattttc agtggcgtga ctggtaaatg actgtgtttc atcagcaatg
    16261 ttactgctgg aagcagctgg ttgttcctgc tgcgtggcgg acggcagaga aggtccctgg
    16321 tcatggctgt gccctgtatc gggtgatgtt tcaggatatt ctgtcggagt catggttttt
    16381 tcctcagtgt ggaccggtgt aaaaacgagg ttgccgatca ccagtgcctc gcggatttcc
    16441 ggggtgttac tgttgggggt ggaaaccccg atgtgttcta actgggcgtt gatgacatgc
    16501 tgaaaagcgg cccgtccccg gcttgcgtcc aggtcattga aatcggtcag cccctgcgct
    16561 ttttcggcat cggtgaacgc aggaaagatg accgttcctc ctgtcagttc tgcggctttt
    16621 gttgcactga cgataccttt gttaacccgg attgcatgat cgttgtcggc acagaagata
    16681 aaggggcttt gcgtccactt ttgccgggca ttttcggcaa cggcaatcat gttgcctcca
    16741 tccaccgtca tcagtaccgg aaggcccgtg gcttcgtgca gggaggccgc agtggcatag
    16801 ccctcggcaa acagtaccgg ctgaccgttg cgcggtgtgc ccagcgcaaa ccagttaccg
    16861 gttttctctg aatctttcag gatgcgggca tccttccccc cggtgaccgg aatacgctgg
    16921 taagagcgta tcgcgccgtt acggttactg aagggaataa ccagttcatt tttgttgttt
    16981 acccgcaccc ccggtgcagc ctgaatgcct ttccgggtca ggtattcatg tgctgtggcc
    17041 tgcggccatt tgttgacata gcggcgggca tacgcggcct gtcggttgta ctgtgctttc
    17101 agttcccgct ccgcgtcctc cctgcgttgc attgaatgcg ctttcaggtg gagtcttgcg
    17161 cggggatctg tctgttctcc gccggagaag gtccaggtga tcggcgaatc atccgcgctg
    17221 cgataatccc tgtaccagcc ggcaggtctg ccgtccagga atccgcgata tgcgccactt
    17281 ttttgtcctt ttttgtcatc ggcggtcggg acacggtgga ttttcccgtc catgaccggc
    17341 agttctttca gaaccagtcc ggcattctcc agaacctggg caaactcggt gaccggatca
    17401 ctgccgttca tggagacgtc ctgtggccgg ggcagccagc ggtccagcct ggacaggtcc
    17461 gcccccgggc gggcgaacca gagtttgtgc tccttgtccc agatgacggg cgtgtgcccg
    17521 ttcatcgggg gatgtgcttt tttgagatct tctatttcat ctgcaggcac ggcaagccag
    17581 gtgcggtagc gggatacaga cggcatgagt gcttctcctg aaaaaagacg gaaaaaaacc
    17641 accgccggaa gccggggggg ctggggggat agaaaatcgg gggagaaatg tttgtcaggg
    17701 tttccagtct gtacccttat tccagcgatc ctgccagtgt tcaagatatt gtgcggctac
    17761 atccggcata ttcttgagca caacgacatt ctctgaattc ttgcgtgcag cagcacgaga
    17821 gaagttatag gagccagttt caaccgtgtt tccgtcaaca atgatgactt tgtcgtgatg
    17881 gatggggaag gcatctacag tccggagagg aatgtccagc aggttgatat atttcatcgc
    17941 ttcctgactt gcccggttcg tattcccctt gtcatcaaca acaatacgga cgtccactcc
    18001 gcgttttttc gcttttgcca gcgcatgcat tacatccggg tcggtgaagg aatatgccat
    18061 catcctgatg gagctttccg cactgttgat tgtgtcaagc accagagcgg atgcgcttcc
    18121 ttcaggagaa aagccgacct tcacggatgg cgtgtcgatc attgcaaacg atggtgtcgt
    18181 gaacagtaac gggcatgcca gtagcgatac ccggagcaga gtaacgatag tttttttacc
    18241 tgacataaag actccagaat ttaatcgagg ccagtccagt ctcgtggtga ttccgtatgg
    18301 tggcgatacc accatggacg accggaggcg atggcccctc tcagaatgct gaaaatgcgt
    18361 gtacagatag tcctgacagt ccagcctgaa cggtcgagaa taacgaaaaa taaaatgatg
    18421 ccggtgcaaa tccagaaggt tgttttggag ggaaaggggc atataaacag ataaatcatg
    18481 gtgaacggca gaggtacgcc ctggatgttg actggcggtg ttgcatatcg ccagaaacct
    18541 tctcttttca tgaccgtaac tcccctggcg ggataaaaag aatcgcttct cttcggtcaa
    18601 cttcattccg gatatacatt tccagaatct ggtcccggat acggcgcttc tcctgccgga
    18661 ttatggcatc aatgtgatgc ccccactctg catgcggcat tccggaaaga ttgtcgcgaa
    18721 gctcatcgtt aatgacgata tattcccgga cggctttacg accaccgtca tttgttctga
    18781 ccagaacctg aaccagaatg aactgcaaca gtgacaggac agcccaggcc atcgaatccc
    18841 tgatgacagg gggaaacagc ccaagaagac gtgccagcgt ttcaccagga gatttcgtat
    18901 gcatggtgct caggcagtaa tgcccggtgt tgcctgcctg taccgctgcg tcggcggttt
    18961 cgttatccct gatctcacca acaccgataa tttcgggatt acgtcgtaca gcagaacgca
    19021 gtccggcagc aaaactgacc acatcacgcc cgatttctgc ctgatgcggt gggagcagat
    19081 cgttttcatt tcccaggata tattccaccg ggtcttcata tgtcacgatt ttggcgtcgg
    19141 ggaaattgtc cataatgtaa cggtacagtg ctgaacacag cgttgatttt cctgacccgg
    19201 tttcaccaca tacaaatccc agaccgcttt tgcagaccat tgcttcaagc aggtctggtt
    19261 caatgttcat ggagagaatg tccggtattt ctgtcgggat gacacgcatg gttattgaga
    19321 ttgctttctc ctccgcacca gatgtgccct gaatgaggtg agagcgtaac cggatgcgtt
    19381 ctccgcgttt aagaccatag cgcccggatg catccccgtt aatctgtatc gtcctgtcaa
    19441 ccggattgcc tgccagaacg cggggaatga cttcccgacc gaacagttca tcaatcaggg
    19501 aggacatgag tgtggttggt aacgttgcac tggaacaacg cacccgtcgg ccaaagcgac
    19561 tgacagatac aggtgacccg ccggtcagat ccacatcact gactttatgt gcggcacacc
    19621 agacaaagaa atgccgtagt tcatctgcag tgaaacgctg aaacggaaaa agaccaaact
    19681 catcaggaat attcatcggg agcctccttc agtgcctata agcggacgcc agtgagattt
    19741 ctgaagattg aattccgcat gatttttaag gcgacgaaca cgatctccag tgaccagttt
    19801 tttcccgtca cctgtaacgg tttgcatttg atcagaaaca tccggacggg tgatcatgcc
    19861 ctgacgccag agaagtgagt acagcatcat gccccggtaa tcacgggtga gggttttctg
    19921 gttggcttca agcgtcaggt cggcgttttg tcttccttcc tgccatccct gccgtaatgc
    19981 ggtttcccac acttttcgtt gcacgctgtc ttctggaacg acgctgcctt ctgtacccgg
    20041 agtggctgtt gttgaaagcc cccggagaag ccagtcacgc cagcccggtg gattgctgac
    20101 aaagcgttca gggcgaatga tggtccagac ccgggaagag gtccggattt gatctggcgt
    20161 gatatgagct acgtcctgtg cttcatcaat aaccggtggt agccagcctt ccgggctaat
    20221 cagtggccgg aaatcgtata aggcattaag tgtgctttcc cgggcgttga gcgcctgaat
    20281 cagctcccat gaacgctgag ccttgccacc acgaaatcca agcgttttac ctgcgtctgt
    20341 cagcattttc cagcgtgttt ctgacagact atccggcttt tcacttcgtg gtttaagcca
    20401 tgcattaatg tcaggtggtg gcgtattgac accacctggc gagatggtag gggaagaaga
    20461 acaaccagta agcagtatta ctggcaaaag gagagaggtg agtgctttca ttttttccgg
    20521 ttctccggct gcatgaagct gacctgtaac aagcccggat attgatggat tgttgctctc
    20581 caggctgtct gggcttcaat aagtctcaac gtattggaaa acgtcatatc ccggacgtga
    20641 aggttgactg gtaacggcag gcggacacca ttgtagttaa actgcatccc cctgactctg
    20701 gccagttcgt tcagtaattc aattgcatca ccttcccagt caaaggaaag gcgatcgctg
    20761 ttagctgtca gaagatgaga ccccgttgaa accggaagtt ggccggaaaa tacacctttg
    20821 tcccataatg tgtactgagt gtgccgggta accgccacat cagaggcagc accgtagtta
    20881 cgcggaacag gagtatgctg agaaggggag ttgcagccag ccagaaaaag ggcggcaaga
    20941 caccccgcag gtataatacg gttcataata attcctgagc agttaaatca gaatgggata
    21001 taccggtgct ccggtacaga caggatgatg tttgtttccg gctatacttg cgttaccgga
    21061 aataaatgcc agtgtggcca ggtaaatagc ctcatagttt actgaaggtg attattcctg
    21121 atactggctg gttgatgtgt cgtcagacat aaaacgaata tgagcgtaat ctttaacgct
    21181 ccgcgccgaa tcgatccatg atggatgact cgacgattta tgaatcgaac gtaccggcgc
    21241 ttgtccctga aacagttccc ggccctcgtc cggataacct gcaattcaga caggcacatg
    21301 tgcccgtaca ctcttgtatc ttactgagta tatcagaatt tatgtaaatt caaatatcct
    21361 ctatgcctct ttgttgtatt ttccgtcaag tatgagccgg gtaccatcgt aatgcatgat
    21421 cagggcatca aggtacgccg gtttgaattt cttgccgagc ctatctctgg ccagaaatga
    21481 taaacaatga ttatgtgtca ttattactat gttcctttta tga
//
LOCUS       DAFAVF010000066        21388 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFAVF010000066 DAFAVF010000000
VERSION     DAFAVF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.066"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(28..1278)
                     /gene="icd"
                     /locus_tag="LGK42_002470"
     CDS             complement(28..1278)
                     /gene="icd"
                     /locus_tag="LGK42_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBK2658710.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1450..2103
                     /gene="rluE"
                     /locus_tag="LGK42_002471"
     CDS             1450..2103
                     /gene="rluE"
                     /locus_tag="LGK42_002471"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBK2658711.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2113..2574
                     /gene="nudJ"
                     /locus_tag="LGK42_002472"
     CDS             2113..2574
                     /gene="nudJ"
                     /locus_tag="LGK42_002472"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBK2658712.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2628..3734
                     /gene="mnmA"
                     /locus_tag="LGK42_002473"
     CDS             2628..3734
                     /gene="mnmA"
                     /locus_tag="LGK42_002473"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK2658713.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3770..4411
                     /gene="hflD"
                     /locus_tag="LGK42_002474"
     CDS             3770..4411
                     /gene="hflD"
                     /locus_tag="LGK42_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBK2658714.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4415..5785
                     /gene="purB"
                     /locus_tag="LGK42_002475"
     CDS             4415..5785
                     /gene="purB"
                     /locus_tag="LGK42_002475"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK2658715.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWLQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5953..6624
                     /gene="phoP"
                     /locus_tag="LGK42_002476"
     CDS             5953..6624
                     /gene="phoP"
                     /locus_tag="LGK42_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBK2658716.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6624..8084
                     /gene="phoQ"
                     /locus_tag="LGK42_002477"
     CDS             6624..8084
                     /gene="phoQ"
                     /locus_tag="LGK42_002477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBK2658717.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8160..9281
                     /gene="roxA"
                     /locus_tag="LGK42_002478"
                     /gene_synonym="ycfD"
     CDS             8160..9281
                     /gene="roxA"
                     /locus_tag="LGK42_002478"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBK2658718.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDIPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9427..10656)
                     /gene="pepT"
                     /locus_tag="LGK42_002479"
     CDS             complement(9427..10656)
                     /gene="pepT"
                     /locus_tag="LGK42_002479"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK2658719.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10906..12042
                     /gene="potA"
                     /locus_tag="LGK42_002480"
     CDS             10906..12042
                     /gene="potA"
                     /locus_tag="LGK42_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBK2658720.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12026..12883
                     /gene="potB"
                     /locus_tag="LGK42_002481"
     CDS             12026..12883
                     /gene="potB"
                     /locus_tag="LGK42_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBK2658721.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12880..13674
                     /gene="potC"
                     /locus_tag="LGK42_002482"
     CDS             12880..13674
                     /gene="potC"
                     /locus_tag="LGK42_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBK2658722.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13671..14717
                     /gene="potD"
                     /locus_tag="LGK42_002483"
     CDS             13671..14717
                     /gene="potD"
                     /locus_tag="LGK42_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBK2658723.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14775..15236
                     /locus_tag="LGK42_002484"
     CDS             14775..15236
                     /locus_tag="LGK42_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBK2658724.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15233..15571
                     /locus_tag="LGK42_002485"
     CDS             15233..15571
                     /locus_tag="LGK42_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658725.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNG"
     gene            15674..16021
                     /locus_tag="LGK42_002486"
     CDS             15674..16021
                     /locus_tag="LGK42_002486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658726.1"
                     /translation="MAVLQAQILKQPTPPLTYYPHPGQQHENAPPGSTNTLGFIAWAA
                     TFILFSWIFCYTTRFIYALKFAVAMTIANMGYQALCLYIDNSFAITRISPLWAGLIGV
                     CTFIAALLLTSKR"
     gene            complement(16160..16981)
                     /gene="cobB"
                     /locus_tag="LGK42_002487"
     CDS             complement(16160..16981)
                     /gene="cobB"
                     /locus_tag="LGK42_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK2658727.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16997..17908)
                     /gene="nagK"
                     /locus_tag="LGK42_002488"
     CDS             complement(16997..17908)
                     /gene="nagK"
                     /locus_tag="LGK42_002488"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBK2658728.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17937..19181)
                     /gene="lolE"
                     /locus_tag="LGK42_002489"
     CDS             complement(17937..19181)
                     /gene="lolE"
                     /locus_tag="LGK42_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBK2658729.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19181..19882)
                     /gene="lolD"
                     /locus_tag="LGK42_002490"
     CDS             complement(19181..19882)
                     /gene="lolD"
                     /locus_tag="LGK42_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBK2658730.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19875..21074)
                     /gene="lolC"
                     /locus_tag="LGK42_002491"
     CDS             complement(19875..21074)
                     /gene="lolC"
                     /locus_tag="LGK42_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBK2658731.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
BASE COUNT         5304 a         5546 c         5322 g         5216 t
ORIGIN      
        1 cgttgcaata ttaaacacat gtagtgatta catgttcttg atgatcgcat caccaaattc
       61 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      121 cgcattgatt gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      181 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      241 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      301 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      361 accattcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      421 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtgt tcgggttttt
      481 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aactcttcac gcgccagctg
      541 gtagccccaa tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      601 agtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      661 ggtgccttct tccgaacacg gcttaatacc gataccacaa tgttccggga agcgaatttt
      721 cttcaccccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtctgc
      781 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      841 cagttcaggg tgtttaaccg ggcttggagt gccttgatag taacgtaccg gacgcaggca
      901 gatgtagaga tccagttcct ggcgcagggc aacgttcaga gagcgaatac cgccaccaac
      961 aggagtggtc agcggacctt taatggcaac gcgatattca cgaatcagat caagagtttc
     1021 agcaggcagc cagacgtcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1081 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1141 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1201 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1261 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1321 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1381 atttgtaatt ggcttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1441 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1501 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaacc tgaaaaccag
     1561 cccacgcgtg tgatcctgtt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1621 gccggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgt
     1681 cttgaccgcg atagcgaagg gttactggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1741 ttaacccagc cgggcaaacg caccgggaaa atctattatg tgcaggtgga aggtattccc
     1801 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1861 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1921 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     1981 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2041 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2101 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2161 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2221 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2281 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2341 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2401 catgacagcg atatcgactg ctgtcgttgg gtcagcgccg aagaaatttt acaggcgtca
     2461 aatcttcgtt cgccgctggt ggcggaaagt atccgttgtt atcaaagcgg gcaacgctat
     2521 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2581 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2641 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2701 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2761 gtgaggaata ttgcacagcg gcagcggatc tggctgatgc ccaggctgtc tgcgacaagc
     2821 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2881 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaaag
     2941 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3001 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3061 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3121 aaagcctgtt cccggtcggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3181 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3241 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3301 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3361 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3421 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3481 tcgggttgat tgcccagcag ttgcactggg tcgatcgcga accattcacc ggcactatgc
     3541 gttgcacggt aaaaacccgc tatcgccaga ccgatatccc ttgcaccgtc aaggcgctgg
     3601 acgatgatcg cattgaagtg attttcgatg aaccggttgc cgccgtgacg ccgggccagt
     3661 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3721 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3781 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3841 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3901 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     3961 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt cgccagggct taaacgccga
     4021 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4081 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4141 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4201 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4261 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4321 cggcggcgga cgtctgcaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4381 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4441 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4501 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4561 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4621 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4681 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4741 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4801 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcttca
     4861 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4921 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     4981 ccgtatggag cgccagtacc gccagcttaa tcaggtggag atcctcggca aaatcaacgg
     5041 cgcggtcggt aactataacg cccatatcgc cgcttacccg gaagttgact ggcatcagtt
     5101 cagcgaagag ttcgtcacct cgctgggtat tcagtggaac ccgtacacca cccagatcga
     5161 accgcacgac tacattgccg aattgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5221 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5281 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgattg acttcgaaaa
     5341 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5401 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5461 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5521 aaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg ctgagccaat
     5581 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagctgac
     5641 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5701 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5761 caccatggtt gatgagctga aataaactcg tatcagtgcc ggatggcgat gctgtccggc
     5821 ctgtttatta aaattatcca ctttttcttt tttcacttta cctcccctcc ccgctggttt
     5881 atttaatgtt tacccccata aacacataat cgcgttacac tattttaata attaagacag
     5941 ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt
     6001 aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc
     6061 gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgcctgac
     6121 gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg atgtttcact gccgattctg
     6181 gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat
     6241 gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg
     6301 cggcgtaata gcggcctggc ttcacaggtc atttcgctac ccccgtttca ggttgatctc
     6361 tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc
     6421 attatggaaa cgttgatacg caataatggc aaagtggtga gcaaagattc gttaatgctc
     6481 caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt
     6541 ctgcgcaaaa aaattcaggc acaatatccg caagaagtga ttactaccgt tcgcggccag
     6601 ggctatttgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcac
     6661 tgcgggtacg ttttctgttg gcaacggctg cggtagtact ggtgctttcg cttgcctacg
     6721 gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac
     6781 gtggcgagag caatctgttc tatacccttg cgaagtggga aaacaataag ttgcatgtcg
     6841 agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg
     6901 ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaagatgatc cagcctgact
     6961 ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc
     7021 tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg
     7081 cggagatgac ccactcggtg gcagtaaacg tctacccggc aacatcgcgg atgccaaaat
     7141 taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga
     7201 gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg
     7261 ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag
     7321 aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa
     7381 acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca
     7441 ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc
     7501 tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc
     7561 gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc
     7621 tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca
     7681 aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg
     7741 tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat
     7801 attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg
     7861 tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc
     7921 aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca
     7981 ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg
     8041 aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccatacttca
     8101 cgcattacgt taagcatccg ttataatcgg ttgcagatac cagcctgtgg atgcttaaca
     8161 tggaatacca actcactctt aactggcccg attttcttga acgtcactgg cagaaacgcc
     8221 cggtggtgtt aaaacgcggc tttaataatt ttattgaccc gatctctcca gacgagttgg
     8281 cgggtctggc gatggaaagc gaagttgaca gtcgactggt cagtcaccag gatggcaaat
     8341 ggcaggtcag ccacggcccg ttcgaaagct acgatcatct cggtgaaacc aactggtcat
     8401 tactggtaca ggcagtgaac cactggcatg agccgaccgc cgcgctgatg cgaccgttcc
     8461 gtgaactacc ggactggcgt attgatgatc tgatgatctc tttttctgta ccgggcggcg
     8521 gcgtcggccc gcatctcgat cagtacgacg tgtttatcat tcagggtacc ggtcgtcgtc
     8581 gctggcgagt gggcgaaaag ctgcaaatga aacagcactg tccgcacccg gatctgttac
     8641 aggtcgatcc gttcgaagcc atcatcgatg aagagctgga gcctggcgat attctttata
     8701 ttccgccagg attcccgcat gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg
     8761 gttttcgcgc gccgaatacg cgggaactga ttagcggatt tgccgattat gtgctgcaac
     8821 gtgaactggg cggcaactac tacagcgatc cggatattcc acctcgcgct catcctgcgg
     8881 atgttctgcc gcaagagatg gataaactgc gtgagatgat gctcgaattg atcaaccagc
     8941 cggaacactt taagcaatgg tttggcgagt ttatatccca gtcacgtcat gaactggata
     9001 tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta cgatgcgctg aaacaaggtg
     9061 atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca
     9121 atggtgagaa gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc agcaacattg
     9181 cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc gcaatgctcg
     9241 cggcgctggt aaatagcggg tattggttct tcgaagggta agtttggatt taagccggat
     9301 gtggtgctga ttgcgcctta tcgtgcctac atgcactgaa cgcctgtagg cacgataagt
     9361 atgtggacca gtgtcaatat aacggtttca tcccggtaat tggcagtcca catacgcatc
     9421 gggcaatcac gacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc
     9481 tttttccata ccttccagag tcacaaactc atgcttacca tgatagttgt aaccgccagt
     9541 gaacaggttc gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg
     9601 gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag
     9661 aatatgcgga tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa
     9721 ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt
     9781 acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtaat gcatatcggc
     9841 ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat
     9901 ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt
     9961 taccatcact cctttcgccg tgcccgggtg aacattgtta ccgacaattt tgatattgac
    10021 cgacgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccat caacagtgta
    10081 agcccagcgg gcatcgaagg cgtcaacatc aaaatgtttc gcccctttgc ccacttcttc
    10141 atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc
    10201 cagcgcggtc atgatttctg caatacctgc tttgtcatcg gcacctaaca aggttttacc
    10261 atcggtagta atcagcgtct gacccagtag ctgatgcagc accgggaaca taaccggtga
    10321 taaaacttca tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg
    10381 cggattcaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat
    10441 cgccgggata ttgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact
    10501 taaggtcaca ttgataagcc ccatctcttc gagctgctct ttcagtagat gcaataactt
    10561 ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga
    10621 cacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac
    10681 aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag
    10741 cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa
    10801 agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt
    10861 ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa
    10921 aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa
    10981 tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc
    11041 ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt
    11101 ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg
    11161 gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc
    11221 gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg
    11281 ccccgcgtta tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg
    11341 catcagctct ctggtggtca acagcaacgc gtcgccattg cccgtgcggt ggttaacaag
    11401 cctcgtcttt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa
    11461 atgcagaacg agctgaaagc gttacagcgt aagcttggca ttacattcgt cttcgtgact
    11521 cacgaccagg aagaagcact caccatgtca gacaggattg tggtgatgcg cgatggtcgc
    11581 atcgagcaag acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc
    11641 gggttcattg gcgaaatcaa tatgtttaac gcaacggtca tcgaacgtct cgacgagcag
    11701 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
    11761 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
    11821 gacgacaacc acgctgaagg gctaattggt tacgttcgcg agcgtaacta caaaggcatg
    11881 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
    11941 aatgaagacg atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
    12001 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
    12061 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
    12121 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa
    12181 ctatacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
    12241 gctgatcgcc accctcgcct gcctggtgct gggctacccg tttgcctggt ttctggcgaa
    12301 gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccct tctggaccaa
    12361 ctcattgatt cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
    12421 gtttttgctc tggctgggcg ttatagatac accaattcgc atcatgttca cgcccagtgc
    12481 ggtgattatc ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtattccag
    12541 tatcgaaaaa ctggataagc cgttgcttga ggcagcgcgc gatctcggtg ccagcaagtt
    12601 acagactttt atccgtatca ttattccact gacgatgccg ggaattattg ccggatgtct
    12661 gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa
    12721 aaacctgctg atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt
    12781 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
    12841 gcgcgcttct cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc
    12901 ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg
    12961 aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat
    13021 agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg
    13081 gtgttttcgg cgacatttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt
    13141 tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat gatgtcacca
    13201 gatatcgtga tggcgatttc tctgctggtg ctgtttatgc tgctgggtat tcagcttggc
    13261 ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt ggtgacggtg
    13321 tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc
    13381 agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgcctgc ggtggcggcg
    13441 ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc gttcgtcacc
    13501 ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg
    13561 ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc
    13621 agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat
    13681 ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg
    13741 atgacaacaa cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg
    13801 aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa
    13861 ccatgtacgc caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa
    13921 cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt
    13981 taacgaactt cagcaatctc gatccagaca tgctcaacaa gccgtttgac ccgaataacg
    14041 attactccat tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg
    14101 atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc tgagtacaaa ggcagcctgc
    14161 tgttgaccga cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg
    14221 gtaacaccac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc
    14281 caaacgtcgc agcgtttaac tccgataacc cagctaaccc gtacatggaa ggcgaagtta
    14341 acctcggcat gatctggaac ggttctgctt tcgttgcacg gcaggcgggt acgccaattg
    14401 acgtggtgtg gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa
    14461 atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg
    14521 caaaacaggt tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt
    14581 taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg
    14641 gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc
    14701 tgaaagcagg acgttaattc agcaagcggg cggtggcatt accgcccgtt ttttatttag
    14761 caaggatgtg gctaatgagt caggacagta aagtgttatc ccgcgctttt ctcggtatcg
    14821 gtctgatatt gctcgttatt tctgcaatca tcttttatta ccattttact tttaccaaaa
    14881 gcgcggttca tacagaagga atcattgttg atgccgtttg gtacaacaat cactcaaacg
    14941 acgtcgatga taatggttcc tggtatccag tggttgcctt tcgccctacg ccagattaca
    15001 cgctaatttt caattcgaat atcggtagtg atttttatga agatagcgaa ggtgacagag
    15061 tcaatgtcta ttatccgcca gggcatccag aacaagccga aataaacaat ccctgggtta
    15121 atttttttaa atggggcttt gttggcatag caggcattat tttcggtagt gtcggtctga
    15181 taatcacgct cccctccacc aaaaaatctc gcaggaaacg gaagtcccgc ccatgaaaaa
    15241 attcattata ctgctcagtt tactgatact cctgcctctg gtagcaacca gtaaaccatt
    15301 gattccaata atgaagacac tttttacgga tgttaccggt actgtaccgg atgctgaaga
    15361 gatcgcacgt aaagccgagc tttttcgcca acaaacggga attgcaccgt ttattgtgat
    15421 tttacccgac attaataatg aagccagtct cagacaaaat ggtaaagcga tgctggcgca
    15481 tgcgtcatct tcattgagta atgtaaaagg aagtgttctg ttactattta ctacccgcga
    15541 accacggtta attatgatca ccaacggcta ggttgaaagt ggtctggacg ataaacatct
    15601 cggccttctg atagaaaatc acacgctggc ttatttaaat gcagatctct ggtatcaggg
    15661 aatcaataat gcattggctg ttctacaagc acagatatta aaacaaccga cgccgccact
    15721 gacgtattat ccgcatccag gacaacagca tgaaaatgct ccccccggca gcaccaacac
    15781 attaggtttt atcgcctggg ctgcgacgtt tatcttattt tcctggatct tttgctacac
    15841 gactcgtttt atttatgcgt taaaatttgc agtggcgatg actattgcca atatgggtta
    15901 tcaggcttta tgtttgtata tcgataatag ctttgcgatt accagaatat cgccactctg
    15961 ggcagggcta attggtgtct gtacgtttat tgccgcattg ttattgacga gtaaacgata
    16021 aaagaggccg gaaaagtgcg cgtgtattat gccggatggg gcgcgaacgc cttatccggc
    16081 ccacaaaacc cgcaaattca attaattgcg tccccttgta ggcctgataa gcgtagcgca
    16141 tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca
    16201 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga
    16261 gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg
    16321 gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat
    16381 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
    16441 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
    16501 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
    16561 tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
    16621 cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
    16681 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
    16741 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
    16801 gaaaggtacg aatacctgat tccgcagaaa tccctgcccc tgtcagtacg agtactcttg
    16861 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
    16921 cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
    16981 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
    17041 catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag
    17101 gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
    17161 cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct aataaatcca
    17221 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
    17281 cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag
    17341 acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca
    17401 accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
    17461 agcttttacc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg ccggtgccga
    17521 gaatcaaccc catcaccagt ggatattgag taaattcatc atcccaggct tctgaaaggg
    17581 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
    17641 cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg
    17701 tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag
    17761 cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
    17821 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
    17881 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
    17941 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    18001 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    18061 tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg accgatcaac
    18121 ttttcaatcc actcaataat cggggtaagt tgcagtgaaa ccaccacacc gataatcacc
    18181 ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    18241 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    18301 gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    18361 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    18421 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    18481 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    18541 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    18601 tgcaaaatac cggcaacgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    18661 ttcgagttgg ggatcataat cgacacccaa tccccctgct tcactttcag cgcatccgcc
    18721 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca cgcatcgccc
    18781 tggacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc
    18841 tggattgcgc gaagattcac gccactttcc accagcccgg tgaaattgat atacggcgcg
    18901 gcagcggcaa tacccggcac tttttgcacg tgatccagtg cttcctgcca gttagtccac
    18961 ggctgatcca ccgcctcgat ttcaccatgc ggcaccaccg ccagaatacg gttgttcagt
    19021 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    19081 atgccaatgg tagaaataac ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga
    19141 ctaaaacgca ggccaattaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    19201 tcagttccgc cgtcagacga ccatcacgca tttctaactg gcggctcata cgtttcgcca
    19261 gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa
    19321 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggt tcatccgcca
    19381 gtaccaggcg cgggttattc accagcgcac gggcaatcgc cacacgctgg cgttcgccgc
    19441 cagaaagttc agatgggcgg tgattcgcac gatgatccag ccccaccgct tttaacatct
    19501 caagtgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagc ggcatagcca
    19561 cgttttccag ggcggtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    19621 tctggttgcg cagctctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa
    19681 agatcacatc gccggaggtt ggcgtatcca gcccgcccaa caagtgcagc aaggtgcttt
    19741 taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgccgacg ctgaaactga
    19801 cattgtgcag cacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    19861 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgagtggc ggcagcgcgc
    19921 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    19981 acctgtaaag gttcgatagc gaccggcagc gccgcgccat caagcaggac gccgattatc
    20041 ggcatcagat tatttaactg gctggcaagc agggcgccaa gcgccgctcc gaggatcgca
    20101 ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    20161 aagccttgcg tttgcaggat cgctacttcg ccctgcttct ccattaccat cagccctagt
    20221 gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagtaa acccatcata
    20281 tttttttcca tgcgtacggc ctggaacaac tcgcctttac gatcacgcca gtcctgccat
    20341 ttgctgcctt caggcagttt ttgctgactt aatgagtcga ctttcagcgg ctcatccagc
    20401 cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca
    20461 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    20521 ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc agatggtacc
    20581 atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg
    20641 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggtgta
    20701 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    20761 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    20821 actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    20881 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    20941 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    21001 aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    21061 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    21121 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    21181 gtgaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct
    21241 gaaaaatcat ccaatacaga cagataccca ttacacatag ggtcataacc cgcaggatca
    21301 accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
    21361 accagatcaa agggttatgt atttgtct
//
LOCUS       DAFAVF010000067        21128 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFAVF010000067 DAFAVF010000000
VERSION     DAFAVF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.067"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..98
                     /locus_tag="LGK42_002492"
     CDS             <1..98
                     /locus_tag="LGK42_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBK2658732.1"
                     /translation="ITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            112..1404
                     /locus_tag="LGK42_002493"
     CDS             112..1404
                     /locus_tag="LGK42_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2658733.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            1425..2306
                     /locus_tag="LGK42_002494"
     CDS             1425..2306
                     /locus_tag="LGK42_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK2658734.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2293..3135
                     /locus_tag="LGK42_002495"
     CDS             2293..3135
                     /locus_tag="LGK42_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK2658735.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3166..4218
                     /locus_tag="LGK42_002496"
     CDS             3166..4218
                     /locus_tag="LGK42_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK2658736.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4237..5025
                     /locus_tag="LGK42_002497"
     CDS             4237..5025
                     /locus_tag="LGK42_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK2658737.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTTCGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            5035..6090
                     /locus_tag="LGK42_002498"
     CDS             5035..6090
                     /locus_tag="LGK42_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2658738.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6087..8354
                     /gene="ycjT"
                     /locus_tag="LGK42_002499"
     CDS             6087..8354
                     /gene="ycjT"
                     /locus_tag="LGK42_002499"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBK2658739.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            8351..9010
                     /gene="pgmB"
                     /locus_tag="LGK42_002500"
     CDS             8351..9010
                     /gene="pgmB"
                     /locus_tag="LGK42_002500"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK2658740.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            9024..10106
                     /locus_tag="LGK42_002501"
     CDS             9024..10106
                     /locus_tag="LGK42_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2658741.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10151..11056
                     /gene="ompG"
                     /locus_tag="LGK42_002502"
     CDS             10151..11056
                     /gene="ompG"
                     /locus_tag="LGK42_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBK2658742.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11167..12165)
                     /locus_tag="LGK42_002503"
     CDS             complement(11167..12165)
                     /locus_tag="LGK42_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2658743.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            12320..13717
                     /locus_tag="LGK42_002504"
     CDS             12320..13717
                     /locus_tag="LGK42_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBK2658744.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13714..14775
                     /locus_tag="LGK42_002505"
     CDS             13714..14775
                     /locus_tag="LGK42_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBK2658745.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14923..16464
                     /gene="tyrR"
                     /locus_tag="LGK42_002506"
     CDS             14923..16464
                     /gene="tyrR"
                     /locus_tag="LGK42_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBK2658746.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16508..17014)
                     /gene="tpx"
                     /locus_tag="LGK42_002507"
     CDS             complement(16508..17014)
                     /gene="tpx"
                     /locus_tag="LGK42_002507"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBK2658747.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAVSVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17133..18098
                     /gene="ycjG"
                     /locus_tag="LGK42_002508"
     CDS             17133..18098
                     /gene="ycjG"
                     /locus_tag="LGK42_002508"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBK2658748.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEAHVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18073..18801)
                     /gene="mpaA"
                     /locus_tag="LGK42_002509"
     CDS             complement(18073..18801)
                     /gene="mpaA"
                     /locus_tag="LGK42_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBK2658749.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETLALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(18928..19065)
                     /locus_tag="LGK42_002510"
                     /pseudo
     CDS             complement(18928..19065)
                     /locus_tag="LGK42_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            complement(19136..20056)
                     /locus_tag="LGK42_002511"
     CDS             complement(19136..20056)
                     /locus_tag="LGK42_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2658750.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPDIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            20194..21093
                     /gene="pgrR"
                     /locus_tag="LGK42_002512"
     CDS             20194..21093
                     /gene="pgrR"
                     /locus_tag="LGK42_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBK2658751.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         5035 a         5141 c         5794 g         5158 t
ORIGIN      
        1 atattactaa tctgcatggt cgggatctga ttagtgaagt tgatatattg ggtaatgaaa
       61 taacgctgcg cccctggcag gttatgtgga ttaaataaaa aggaacatct catgattaaa
      121 tcaaaaatcg tgctgttatc agcactggtt tcctgcgccc tgatttcagg ctgtaaagaa
      181 gaaaataaaa cgaatgtatc catcgaattt atgcattctt cggtggagca ggagcgccag
      241 gccgttatca gtaaattgat tgcccgtttt gaaaaagaaa accctggcat caccgttaag
      301 caagtgcccg tggaagaaga tgcctataac actaaagtca ttactctttc acgtagcggt
      361 tcgctgccgg aagtgatcga aaccagccat gactacgcca aagtgatgga caaagagcag
      421 cttatcgatc gcaaagcggt tgccacagtc atcagcaacg ttggtgaagg cgcgttttac
      481 gatggcgtac tgcgtattgt gcgtaccgaa gatggtagcg catggaccgg tgttcctgtc
      541 agcgcctgga ttggcggtat ctggtatcgc aaagatgtgc tggcaaaagc ggggcttgag
      601 gagccgaaaa actggcaaca gctgctggac gttgcacaga aactgaatga cccggcgaat
      661 aaaaaatacg gcattgcgct gcctacagca gaaagcgtgt tgacggaaca atccttctcc
      721 cagtttgcgt tatccaacca ggctaacgtc tttaacgccg aaggcaaaat cacccttgat
      781 acaccagaga tgatgcaggc actgacctat taccgcgacc ttgctgccaa cactatgccg
      841 ggttctaacg acatcatgga agtgaaagac gcctttatga acggcaccgc gccgatggcg
      901 atttactcca cctatatcct tccggctgtg attaaagaag gcgacccgaa aaacgtcggt
      961 ttcgtggtgc caaccgagaa aaactctgcg gtctacggca tgttgacctc gctgaccatt
     1021 accgccgggc aaaagaccga agagacggaa gcagcagaaa aatttgtcac ctttatggag
     1081 caggcagaca acattgccga ctgggtgatg atgtcgccag gtgctgcgct gccggtgaat
     1141 aaagcggttg tcactaccgc cacctggaaa gacaacgacg ttattaaggc gctgggtgaa
     1201 ctaccgaatc agctaatcgg tgaactgcca aatattcagg tctttggtgc agtaggggat
     1261 aaaaacttta cccgcatggg tgatgtgacg gggtctggag tggtgagctc catggtgcat
     1321 aacgtcaccg tgggtaaagc cgatctccct ggcacgctgc aagcgagcca gaaaaagctg
     1381 gatgaactga tcgaacagca ctaaaccccg gacaggaatc cgcaatgaac aggctttttt
     1441 caggtcgttc cgatatgccc tttgcgctgt tgcttctcgc gcccagctta ttactgctgg
     1501 gcggtctggt ggcgtggccg atggtgtcga atatcgaaat cagtttttta cgtctgccgc
     1561 tcaatcccaa catcgagtca acctttgttg gggtgagtaa ctatgtgcgt atcctctccg
     1621 atcccggctt ctggcactcg ctgtggatga cggtctggta taccgcgctg gtggtggcgg
     1681 gcagcaccgt gctcgggctg gcagtggcga tgtttttcaa ccgcgagttc cgtctgcgca
     1741 aaaccgcgcg ttcgctggtg atcctctcct acgtaacgcc gtccatttcg ctggtgtttg
     1801 cctggaaata catgttcaac aacggctacg gcattgtgaa ctacctcggc gtcgatcttc
     1861 tgcatctcta tgagcaggca ccgctgtggt tcgataatcc gggcagtagc tttgtgctgg
     1921 tggtgctgtt cgccatctgg cgctacttcc cgtatgcctt tatctcgttt ctggcgattt
     1981 tgcagaccat cgacaaatcg ctgtacgaag cggcagagat ggatggcgct aatgcctggc
     2041 aacggtttcg tatcgtcacg ctgcccgcga ttatgccggt cctggcgacg gtggtgaccc
     2101 tgcgcaccat ctggatgttc tacatgttcg cggatgttta tttgctgacg accaaggtcg
     2161 atattctcgg tgtatatctc tacaaaaccg cctttgcctt taatgattta ggaaaagcgg
     2221 cggcgatctc ggtggtgctc ttcatcatta ttttcgctgt cattctgctg accaggaaac
     2281 gggtgaacct caatggcaac aaataaacgc acactcagtc gcatcggttt ttactgcggg
     2341 ctggcgctgt ttctcatcat cacgctgttt ccattttttg tgatgctgat gacctcgttc
     2401 aagggcgcga aagaggcgat ctcactgcat cctacgctgc tgccgcagca gtggacgctg
     2461 gagcattacg tcgacatttt taacccgatg attttcccgt ttgtcgacta cttccgtaac
     2521 agtctggtgg tgtcggtggt ttcatccgtg gtggcggtat tcctcggcat tcttggggct
     2581 tacgcgcttt cccgcctgcg ctttaaaggt cggatgacca tcaatgccag cttttacacg
     2641 gtgtatatgt tctctggcat tttgctggtg gtgccgctgt tcaaaatcat caccgcgctg
     2701 ggcatttacg acaccgagat ggcgctgatc atcaccatgg tgacgcaaac tctgcccacc
     2761 gccgtgttta tgctgaaaag ctacttcgac accatccccg atgagatcga agaagcggcg
     2821 atgatggatg gcctcaaccg tctgcaaatt atcttccgca tcaccgtgcc gttggcgatg
     2881 tccgggctga tatccgtgtt cgtctactgc tttatggtgg cgtggaacga ctatctgttt
     2941 gcgtcgattt tcctctccag cgccagcaat ttcaccttac cggtgggcct gaacgcgctg
     3001 ttcagtacgc cagattacat ctggggacgg atgatggcgg cctcactggt gaccgcattg
     3061 ccggtggtga ttatgtatgc gctttcggaa cgttttatta agagtggttt gaccgccggt
     3121 ggcgtgaagg gctaaagcgg ccagtttctt tacaaggagt tttaaatgaa aaagttagta
     3181 gccacagcac cgcgtgttgc tgcgctggtt gagtatgaag atcgggcgat tttagccaat
     3241 gaagtgaaaa tccgcgtgcg tttcggcgca ccgaaacacg gaacggaagt ggtcgacttc
     3301 cgcgccgcca gcccgtttat cgatgaagac tttaacggtg aatggcagat gttcactccg
     3361 cgtccggcag atgcgccgcg cggcattgag tttggcaaat tccagcttgg caacatggtg
     3421 gttggcgaca ttatcgagtg cggcagcgac gtaaccgact acgcggtggg cgacagcgta
     3481 tgcggctacg gcccactctc cgagacggtc atcattaacg cggtgaataa ctacaagctg
     3541 cgcaaaatgc cgcaaggcag ctcctggaaa aacgccgtct gttacgaccc ggcgcagttt
     3601 gccatgagcg gcgtgcgcga tgccaacgtg cgcgtgggtg attttgtggt ggtggtaggg
     3661 cttggcgcga ttggtcaaat agccatccaa ctggcgaaac gcgctggcgc atcggtggta
     3721 attggcgtcg atcctatcgc ccatcgctgt gatattgccc gtcgccacgg tgcggatttc
     3781 tgccttaacc ccatcggcac tgacgtaggt aaagagatca aaacgctgac cggcaagcag
     3841 ggtgccgatg tgattatcga aaccagcggt tacgccgacg cgctgcaatc ggcgctccgc
     3901 ggcctggctt atggcggcac catctcgtat gttgcgtttg ccaaaccgtt tgccgaaggt
     3961 tttaacctcg gacgcgaagc ccatttcaat aacgccaaaa ttgtcttctc ccgcgcgtgc
     4021 agcgaaccga acccggatta tccgcgctgg agccgcaagc gtatcgaaga aacctgttgg
     4081 gaactgctga tgaacggtta tctcaattgt gaagatctaa tcgacccggt ggtgaccttt
     4141 gccaatagcc cggaaagcta catgcagtat gtcgaccagc atccggaaca gagcatcaaa
     4201 atgggcgtta cgttttaatt caaaggaatg aaaaaaatga aaataggcac acagaatcag
     4261 gcgttctttc cggaaaatat tctggagaaa tttcgttata tcaaagagat gggtttcgat
     4321 ggttttgaga tcgacggcaa actgctggtc aacaatctcg aagaagtcaa agcggcgatc
     4381 aaagaaactg gtttaccggt gaccaccacc tgcggtggct atgacggctg gattggtgac
     4441 tttatcgaag agcgtcgtct taatggctta aagcagatcg aacgcattct cgaagcgctg
     4501 gcagaagtgg ggggtaaagg catcgtcgtt ccggcagcgt ggggcatgtt taccttccgc
     4561 ttaccgccga tgacctcgcc gcgtagcctg gacggcgacc gcaaaatggt gagtgattcc
     4621 ctgcgcgtac tggaacaggt cgccgcgcgt accggaaccg tggtgtatct cgaaccgtta
     4681 aaccgctatc aggatcatat gatcaacacc ctcgccgatg ctcgtcgtta catcgtcgaa
     4741 aacaatctta aacatgtgca gattatcggc gatttctatc acatgaacat cgaagaagat
     4801 aacctggcgc aggcgctgca cgacaaccgt gacctgctcg gtcatgtgca tattgccgat
     4861 aaccatcgtt accagccggg cagtggcacc ctcgatttcc acgcgctgtt tgaacagctg
     4921 cgcgccgata actatcaggg ttatgtggtg tatgaagggc gtatccgggc agaagatcct
     4981 gcccaggcgt accgtgattc gttggcctgg ttgcgtacct gctaagaggt ctttgtgaaa
     5041 agtgcaatga caagctctcc gctgcgggtc gcgataatag gcgcaggcca ggtggcggat
     5101 aaggttcatg cttcgtacta ctgcacccgc aacgatctgg aactggtggc tgtctgtgat
     5161 agccgccttt cccaggcgca ggcgctggca gataaatacg gtaatgcatc cgtgtgggac
     5221 gatccgcagg ccatgctgct ggcggtgaaa cctgatgtgg ttagcgtctg ctcacctaac
     5281 cgttttcatt acgaacatac cctgatggca ctggaagcgg gctgccatgt gatgtgcgaa
     5341 aaaccgcccg ccatgacgcc agaacaggcg cgggaaatgt gcgataccgc gcgcaaacag
     5401 ggcaaggtgc tggcctacga ctttcaccat cgttttgcgc tcgatacgca acagctgcgt
     5461 gaacaggtga ccaacggcgt tttgggagag atttacgtta ccaccgcccg cgccctgcgt
     5521 cgctgcggcg ttcccggctg gggtgtcttt accaataaag aactgcaggg tggtggcccg
     5581 cttatcgaca tcggcattca tatgctggat gcggcgatgt atgtgctggg ctttccggcg
     5641 gtgaaaagcg tgaatgcgca tagctttcaa aagatcggca cgcaaaagag ctgcggtcaa
     5701 tttggcgagt gggatccggc aacttacagc gtcgaagatt cgttatttgg caccattgaa
     5761 tttcataacg gcggcattct gtggctggaa acgtcatttg cactcaacat ccgcgaacag
     5821 tcgattatga acgtcagctt ttgtggtgat aaagccggtg cgacgctgtt tccagcacat
     5881 atctacaccg ataacaacgg tgaattaatg acgctgatgc aacgggaact ggcagacgac
     5941 aaccgccatt tgcgcagcat ggaagccttt atcaatcacg tacagggcaa gcccgtgatg
     6001 atagccgacg ccgagcaggg gtacatcatc cagcaactgg tggcggcgtt gtatcaatcc
     6061 gcagaaacag ggacgcgtgt ggaattatga ccaggccagt aacgttatca gaaccccatt
     6121 tcagccagca taccctgaac aagtatgcat cgctgatggc gcaggggaac ggctatcttg
     6181 ggcttcgcgc cagccatgaa gaagattaca cgcgccagac gcgagggatg tatctggcgg
     6241 ggctgtatca tcgggcggga aaaggtgaaa tcaacgaact ggtgaacctg cctgatatct
     6301 tggggatgga gattgccata aatggtgagg ttttctcgtt atcccgcgaa gcctggcagc
     6361 gtgaacttga ctttgccagt ggagaattac gccgtagcgt tgtctggcgt accagcaacg
     6421 gcacaggtta caccatcgcc agccgtcgct ttgtttcggc agaccaactg ccgctcattg
     6481 tgctggaaat cactattacg ccactggacg ccgacgcgtc agtgctgatt tcaacaggta
     6541 tcgacgccac gcaaaccaac cacggtcgcc aacatctcga cgaaacccag gtgcgggtgt
     6601 ttggtcagca tctgatgcag gggatctaca ccacccagga tggacgcagt gatgtggcca
     6661 tcagctgttg ctgtatggtg agcggtgatg tgcagcaatg ctataccgcc aaagagcgcc
     6721 gtttgcagca acataccagt gcgcagcttc atgcaggcga gacagtgacg ttgcaaaaac
     6781 tggtgtggat cgactggcgg gatgacaggc aagccgtttt agacgagtgg ggcagcgcgt
     6841 cgcttcgcca gcttgaaatg tgcgcgcagc agagttacga ccaacttctt gcagtatcaa
     6901 cagaaaactg gcgtcaatgg tggcagaaac gtcgtatcac ggtaaatggc ggcgaagcgc
     6961 acgatcagca agcgttagat tatgcgcttt atcatctgcg catcatgacg cctgcccacg
     7021 acgagcgcag cagcattgcg gcaaaaggct taaccggcga aggctacaaa ggccacgttt
     7081 tctgggatac agaagtattt ttgttaccgt ttcatctgtt tagcgatccg acggttgccc
     7141 gaagtttact gcgttatcgc tggcacaact tgccaggcgc gcaggagaaa gcgcgacgca
     7201 acggctggca gggcgcgcta tttccgtggg aaagcgcgcg cagcggcgaa gaagagacgc
     7261 cggaatttgc cgccattaac attcgcaccg ggctgcggca aaaagtggcc tcggcgcagg
     7321 cggaacatca tctggtggcc gatatcgcct gggcggttat tcaatactgg cagaccacgg
     7381 gggatgaaag tttcattgcg catgaaggca tggcgctact tctggaaact gcaaagttct
     7441 ggattagccg cgcggtgagg gttaacgacc gtctggaaat tcatgatgtt attgggcctg
     7501 acgaatatac cgaacatgtc aataataacg ccttcaccag ctatatggcg tattacaacg
     7561 tccagcaggc gctgagtatt gcccgccagt ttggctgtag cgacgatgcg tttatccatc
     7621 gcgccgaaat gttccttaaa gaactgcggc tgccagaaat tcagcccgac ggcgttttgc
     7681 cgcaggatga ttcgtttatg gctaagccgg cgattaatct ggcgaaatac aaagcggcgg
     7741 cggggaagca aaccattctg ctggattatt cacgcgcaga agtgaacgag atgcaaatcc
     7801 tcaaacaagc tgatgtggtg atgctcaatt acatgctgcc ggagcagttc tcagcggcat
     7861 cgtgtcttgc caatctgcaa ttttatgaac cgcgcactat tcacgactcg tcattaagta
     7921 aagcaatcca cggcattgtt gccgcacgct gtggcctgct gacccaaagt tatcagttct
     7981 ggcgcgaggg gactgaaatc gatcttggtg ctgatccgca tagttgtgat gatggtatcc
     8041 atgctgccgc aactggcgct atctggctgg gggcgattca gggttttgcc ggggtgagcg
     8101 tgcgtgacgg tgaattgcat ctcaatccgg cgttacctga gcagtggcaa cagttgtctt
     8161 tccctctgtt ctggcagggc tgcgaattac aggtcactct tgacgcgcag cgtattgcga
     8221 ttcgaacttc tgcgcccgtt tcactgcgtt tgaacggtca gcttatatcc gtggctgaag
     8281 aatctgtttt ctgtttgggt gattttattt tgcccttcaa tgggaccgct accacacatc
     8341 aggaggatga atgaaactgc aaggggtaat tttcgatctg gatggtgtaa tcaccgatac
     8401 cgcgcatctg catttccagg cgtggcagca gattgccgct gaaattggca tcagcattga
     8461 tgcgcagttt aacgaatccc taaaagggat cagccgcgat gagtctctgc ggcgcattct
     8521 gcaacacggg ggcaaagagg gcgactttaa ctcgcaggag agggcgcaac tggcgtatcg
     8581 caaaaatctg ctctatgtcc actcactgcg cgagttgacg gtcagcgctg ttctacccgg
     8641 cattcgctct ttgctggcag atctccgtgc acagcagatc ccggttgggc tggcttctgt
     8701 ctccctgaat gcgccgacga ttttagcggc gctggagctg cgcgagtttt tcaccttctg
     8761 cgcggatgct tcccaactta aaaactcgaa accggacccg gaaatctttc tcgccgcctg
     8821 tgccgggctg ggcgtgccgc cgcaggcatg tatcggcatt gaagatgcgc aggcgggcat
     8881 tgacgccatt aacgccagcg gtatgcgctc ggtggggatc ggcgcgggct taaccggggc
     8941 gcaattactg ttgccttcaa cggactctct gacctggtcg cggttatcgg ccttctggca
     9001 aaacgtatag caaaggaatc aacatggctc agctttcgtt acaacatatt caaaaaatct
     9061 acgataacca ggtgcatgtg gtgaaggact tcaacctgga aattgccgat aaagagttca
     9121 tcgtgtttgt cggcccgtcg ggctgcggta agtcgaccac cctgcgcatg attgccgggc
     9181 ttgaggagat cagcggcggc gatctgttga tcgacggcaa acgaatgaat gacgttccag
     9241 ccaaagcacg caatatagcg atggtgttcc agaactacgc gttgtatccg catatgacgg
     9301 tttacgacaa catggcgttt ggcctgaaga tgcaaaaaat cgccaaagag gtgattgatg
     9361 agcgggtgaa ctgggcggcg caaattctcg gcctgcgtga gtacctgaaa cgcaagccgg
     9421 gcgcgctttc cggcgggcaa cgccagcgtg tggcgctcgg gcgggcgatt gtgcgcgaag
     9481 cgggcgtgtt tttaatggat gaaccgctct ctaaccttga tgccaagctg cgcgtgcaaa
     9541 tgcgcgcaga gatcagcaag ctacaccaga agctgaatac caccatgatc tacgtgaccc
     9601 acgatcagac cgaagcgatg accatggcga cgcggattgt gattatgaag gatgggattg
     9661 ttcagcaagt tggtgcgccg aaaacggttt ataaccaacc agcgaatatg tttgttgccg
     9721 gatttattgg atcaccggcg atgaatttta ttcgtggcac gatcgatggc gataaattcg
     9781 ttacggaaac gcttaaatta accattcccg aagagaaatt agcggttctg aaaacacagg
     9841 gaagtttgca taagcccatc gtgatgggaa tacgtccgga agatattcat ccggacgcgc
     9901 aagaggaaaa taacatttcc gccaaaatta gcgtggcaga attaaccggt gcggaattta
     9961 tgctctacac cacggttggg gggcacgagt tagtggtccg tgctggtgcg ttaaatgatt
    10021 atcatgcagg agaaaatatc actattcatt ttgatatgac gaaatgtcat ttctttgatg
    10081 cagaaacgga aatagcaatt cgctaaatac agggggaagg cattccccca gaataataca
    10141 aggaacaata atgaaaaagt tattaccctg taccgcactg gtgatgtgtg cgggaatggc
    10201 ctgcgcacag gccgaggaaa ggaacgactg gcactttaat atcggcgcga tgtacgaaat
    10261 agaaaacgtc gagggttatg gcgaagatat ggatgggctg gcggagcctt cagtctattt
    10321 taatgccgcc aacgggccgt ggagaattgc tctggcctat tatcaggaag ggccggtaga
    10381 ttatagcgcg ggtaaacgtg gaacgtggtt tgatcgcccg gagctggagg tgcattatca
    10441 gttcctcgaa aacgatgatt tcagtttcgg cctgaccggc ggtttccgta attatggtta
    10501 tcactacgtt gatgaaccgg gtaaagacac ggcgaatatg cagcgctgga aaatcgcgcc
    10561 agactgggat gtgaaactga ctgacgattt acgtttcaac ggttggttgt cgatgtataa
    10621 atttgccaac gatctgaaca cgaccggtta cgctgatacc cgagtcgaaa cggaaacagg
    10681 cctgcaatat accttcaacg aaacggttgc cttgcgagtg aactattatc tcgagcgcgg
    10741 ctttaatatg gacgacagcc gcaataacgg tgagttttct acgcaagaaa ttcgcgccta
    10801 tttgccgctg acgctcggca accactcggt gacgccgtat acgcgcattg ggctggatcg
    10861 ctggagtaac tgggactggc aggatgatat tgaacgtgaa ggccatgatt ttaaccgtgt
    10921 aggtttattt tacggttatg atttccagaa cgggctttcc gtttcgctgg aatacgcgtt
    10981 tgagtggcag gatcacgacg aaggcgacag cgataaattc cattatgcag gtgtcggcgt
    11041 aaattactcg ttctgataat gggctaaatt gccggatgcg gcacgagtac tttatccgaa
    11101 ctataaatgt aggccggata agatgcgcta gcatcgcatc cggcattcag gcaaggtagc
    11161 tggtatttat ttcagcgtca tatgcgtggc aacggtaata ttctgtggtg acggttttcc
    11221 agaaattaag cggaataata actcacagct ttgttgacct aactcctgtg ttgggatatc
    11281 aataccgcct ggcgcaggcg ttaaaataaa cgacagcgtt tcattactat aacccaccac
    11341 cgctaactgc tgcggaatag caatattttt ctctgctgcc gcacgataaa tactcattaa
    11401 tttcaggctg tcagtagcaa acactgcctc aggcaacggt gactggctta ataattcccg
    11461 cgcggcttgc agtgctgttt catgggtata accgccgtca acaatccatt catcacgcac
    11521 tgcaatatta tgcgcagcca ggctctgctt ataaccattt acccgatcca ctgaaacatg
    11581 gacatcaagc ggagcatgca ggcaggcaat attttgatgc ccactttcaa ttaatgcatc
    11641 ggtcaacgca atgctgtcgc caaaattatc ggtatcgaca gaataaacat gggcatattg
    11701 accttcaact ttgccaatca ccacgacagg aatatcgtat ttatcaagtt gggcaaaaaa
    11761 tgactcatcc gctggcgaac ttagcataat aatgccttta atcattttct gcttaatttt
    11821 gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata tcacgtcgaa
    11881 accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa acggattacc
    11941 cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac ccgaagaggt
    12001 cagcgcgcgg gccagcttgt ttggctgata ctgtaattct tcaatggccc gtaacacttt
    12061 ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata cggtggattt
    12121 tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc aggaactccg
    12181 tccgaacgtg aatgctgcac atcttatgga gttcgcccgt cgatgacaac acgggcagtt
    12241 gataatcaat ggcctggccc ccacattcat atccttacga atgatttttt tctggtcttc
    12301 agagcagcac aggacagcaa tgaagcgact taaaaatgaa cttaatgcgc tggtgaatcg
    12361 gggtgtcgac agacatctgc gcctcgccgt aaccggacta agccgcagcg gcaaaacagc
    12421 gtttatcact gcgatggtca atcagttgct caatattcac gccggagcac gcttgccgct
    12481 gttaagcgcg gtgcgtgaag agcgcctgct gggcgtaaaa cgcattcctc agcgtgactt
    12541 tggcattccg cgcttcacat atgatgaagg actggcgcag ttatatggcg atcctcccgc
    12601 ctggccgacg ccaacgcgcg gcgtcagtga aatccgcctg gcgctacgtt ttaaatcgaa
    12661 cgattcgctg ctacgccact tcaaggacac ctccacgctg tatctggaga ttgtggatta
    12721 tcccggcgaa tggttgctcg acctgccgat gctggcgcag gactatttaa gctggtcacg
    12781 ccagatgacg ggcttactca atggtcagcg cggcgaatgg tcggtcaaat ggcgaatgat
    12841 gtgcgaaggg ctggacccgc tagcgcctgc cgacgaaaac cggctggcag acattgctgc
    12901 cgcgtggacc gattatctcc accactgtaa acagcagggg ctgcacttta ttcagcctgg
    12961 gcgctttgtc ttgccgggag atatggcagg tgcgcccgcg ctgcaattct tcccgtggcc
    13021 ggatgtcgat gcctggggcg agtccaaact ggcgcaggcc gataagcaca ccaatgccgg
    13081 aatgctgcgc gagcggttta attattactg cgagaaggtg gtgaaggggt tctataagaa
    13141 tcattttctg cgctttgacc gccagattgt gctggttgat tgcctgcaac ctctcaacag
    13201 tgggccacag gcatttaatg atatgcgtct ggcactgacg cagctgatgc aaagtttcca
    13261 ctacgggcag cgtaccttgt tccggcgttt gttttcgccg gttatcgata agctattgtt
    13321 tgctgccact aaagcggacc atgtgaccat cgatcagcac gccaatatgg tttcattact
    13381 gcaacaactg attcaggatg cctggcaaaa tgcggcgttt gaagggatta gtatggattg
    13441 cctggggctg gcctcggtgc aggcgaccac cagcggcatt attgatgtta acggtgagaa
    13501 aatcccggcg ctacgcggta atcgactcag cgatggcgca ccgctcactg tttatcctgg
    13561 cgaagttccc gctcgtttgc ccggtcaggc gttctgggat aagcaaggct tccagtttga
    13621 ggcgtttcgt ccgcaggtga tggatgtcga caaaccgctg ccgcatattc gccttgatgc
    13681 cgcgcttgaa tttttaatag gagataaatt gcgatgaccg aaccgttaaa accacgtatt
    13741 gatttcgacg gtccgctgga ggtcgatcag aatccaaaat tcagggcgca gcagaccttt
    13801 gacgaaaatc aggcgcaaaa ttttgccccg gccacgctcg atgaagcgcc tgaagaagag
    13861 gggcaagttg aagcggtaat ggatgcagcg ttacgtccga aacgcagcct gtggcgcaaa
    13921 atggtgatgg gcgggctggc tctgtttggc gcaagcgttg tcgggcaggg tgtacagtgg
    13981 acaatgaatg cctggcaaac tcaggactgg gtggcgctgg gtggatgtgc cgctggggca
    14041 ttgattatcg gcgctggcgt aggttctgtg gtaacagagt ggcggcgctt atggcgcttg
    14101 cgacagcgcg cccatgaacg cgacgaagcg cgcgatttgt tgcacagcca cggcacgggc
    14161 aaaggccgcg cattttgcga aaaactggcg cagcaggcgg gtattgatca gtctcatcca
    14221 gcgctgcaac gctggtatgc ctcaatccat gaaacgcaaa acgaccgtga agtggtcagt
    14281 ttgtatgcgc atttggtcca gccagtttta gatgcccagg cgcggcgcga aatcagccgt
    14341 tcggcggcgg aatcaacgtt gatgattgcg gtcagcccgc tggcgttggt cgatatggcg
    14401 tttatcgcct ggcgcaatct gcgtttaatt aatcgcatcg ccacgctgta tggcattgaa
    14461 ctggggtatt acagccgttt gcgtctgttt aagctggtat tgctgaatat cgcttttgcc
    14521 ggagccagcg aactggtgcg cgaagtgggg atggactgga tgtcgcaaga tctcgctgct
    14581 cgtttgtcta cccgcgcagc tcagggaatt ggtgctggac ttctgacggc acgactcggg
    14641 attaaagcta tggagctttg ccgcccgctg ccgtggattg acgatgacaa acctcgcctc
    14701 ggggatttcc gtcgtcagct tatcggtcag gtgaaagaaa cgctgcaaaa aggcaaaacg
    14761 cccagcgaaa aataatgcaa tatcgggtgc tgaccggata tctttacgcc gaagtgcccg
    14821 tttttccgtc tttgtgtcaa tgattgttga cagaaacctt cctgctatcc aaatagtgtc
    14881 atatcatcat attaattgtt cttttttcag gtgaaggttc ccatgcgtct ggaagtcttt
    14941 tgtgaagacc gactcggtct gacccgcgaa ttactcgatc tactcgtgct aagaggcatt
    15001 gatttacgcg gtattgagat tgaccccatt gggcgaatct acctcaattt tgctgaactg
    15061 gagtttgaga gtttcagcag tctgatggcc gaaatacgcc gtattgcagg tgttaccgat
    15121 gtgcgtactg tcccgtggat gccttccgaa cgtgagcatc tggcgttgag cgcgttactg
    15181 gaggcgttgc ctgaacctgt gctctctgtc gatatgaaaa gcaaagtgga tatggcaaac
    15241 ccggcgagct gtcagctttt tgggcaaaaa ttggatcgcc tgcgcaacca taccgccgca
    15301 caattgatta acggctttaa ttttttacgt tggctggaaa gcgaaccgca agattcgcat
    15361 aacgagcatg ttgttattaa tgggcagaat ttcctgatgg agattacgcc tgtttatctt
    15421 caggatgaaa atgatcaaca cgtcctgacc ggtgcggtgg tgatgttgcg atcaacgatt
    15481 cgtatgggcc gtcagttgca aaatgttgcc gcccaggacg tcagcgcctt tagtcaaatt
    15541 gtcgccgtca gcccgaaaat gaagcatgtt gtcgaacagg cgcagaaact ggcgatgcta
    15601 agcgcgccgc tgctgattac gggtgacaca ggtacaggta aagatctttt tgcctacgcc
    15661 tgccatcagg caagccccag agcgggcaaa ccttacctgg cgctgaattg cgcgtctata
    15721 ccagaagatg cggtcgagag tgaactgttt ggtcatgctc cggaagggaa gaaaggattc
    15781 tttgagcagg cgaacggtgg ttcggtgctg ttggatgaaa taggtgagat gtcaccacgg
    15841 atgcaggcga aattactgcg tttccttaat gatggcactt tccgtcgggt tggcgaagac
    15901 catgaggtgc atgtcgatgt gcgggtgatt tgcgctacgc agaagaatct ggtcgaactg
    15961 gtgcaaaaag gcgtgttccg tgaagatctc tattatcgtc tgaacgtgtt gacgctcaat
    16021 ctgccgccgc tacgtgactg tccgcaggac atcatgccgt taactgagct gttcgtcgcc
    16081 cgctttgccg acgagcaggg cgtgccgcgt ccgaaactgg ccgctgacct gaatactgta
    16141 cttacgcgtt atgcgtggcc gggaaatgtg cggcagttaa agaacgctat ctatcgcgca
    16201 ctgacacaac tggacggtta tgagctgcgt ccacaggata ttttgttgcc ggattatgac
    16261 gccgcaacgg tagccgtggg cgaagatgcg atggaaggtt cgctggatga aatcaccagc
    16321 cgttttgaac gctcggtatt aacccagctt tatcgcaatt atcccagcac gcgcaaactg
    16381 gcaaaacgtc tcggcgtttc acataccgcg attgccaata agttgcggga atatggctta
    16441 agccagaaga agaacgaaga gtaagcgcga atatgcctga tggtgcaaca ccatcaggca
    16501 tattaactta tgctttcagt accgccagag ctgcttcgta atccggctcg gtggtgattt
    16561 catccaccag ctggctgaaa atcacattgt cattttcatc aataaccaca acggcacgcg
    16621 ctgccagacc tttcagtggg ccatcagcaa ttgccacacc gtaggcttgc aggaattcag
    16681 cgttacggaa agtggagagg gtgataacgt tgttcagacc ttctgcgccg cagaaacgag
    16741 actgggcgaa cggcagatcg gcagaaatac acagcacaac ggtgttgtcg atctcggttg
    16801 ccagttggtt aaacttacgt actgatacgg cgcaaacacc ggtatcaata ctcgggaaaa
    16861 tgttcagcac tttgcgttta cccgcaaact gaccgagggt gacgtcagac agatcttttg
    16921 ccacgagagt aaaagtctgc gctttgctac ccgcctgcgg gatggaattg gcgactgtaa
    16981 ccgggttgcc ctggaaatga acggtttgtg acatgattat ctttcctgtt tacatatagt
    17041 taacgtcaca cctagtttat gctaactgtc aataacacag caaacgctat ttgcgcttaa
    17101 tccgcagacc accgcgacaa caaggagtaa agatgagaac cgttaaggta tttgaggaag
    17161 cctggccctt acataccccg tttgtgattg cccggggaag tcgcagtgaa gcgcacgtgg
    17221 tggtggttga actggaagaa gaaggcatta aaggcaccgg cgaatgcacg ccgtatccgc
    17281 gttatgggga aagtgatgcc tcggtaatgg cgcaaattat gagcgtcgtg ccgcaattag
    17341 agaaagggct tacacgggag gagttgcaaa aaattctccc tgccggcgca gcacgtaatg
    17401 cgctggattg tgcattgtgg gatctggccg cgcgaaaaca gcagcaatcg ctggctgatt
    17461 tgatcggcat aacgcttccc gggacagtca ccactgcaca gacggtagtc atcggtacgc
    17521 ctgatcagat ggccaatagc gcatcaacac tctggcaggc cggcgcgaaa ttactgaaag
    17581 tgaagctgga taaccatctt atcagtgagc ggatggtggc aattcgcaca gctgtgcccg
    17641 atgccacgct gatcgttgat gcaaatgaat cctggcgtgc agaagggttg gcggcacgtt
    17701 gccagctatt ggcggattta ggcgttgcga tgcttgaaca accgcttcct gcgcaggacg
    17761 atgcggcgct ggagaatttt attcatccgt tgccgatttg tgctgatgaa agttgtcata
    17821 ctcgtagcaa tttgaaggcg ctgaaagggc gctatgagat ggttaatatt aagctcgata
    17881 aaaccggggg gctgacggaa gcgctggcgc tggcgactga agcgcgtgca caaggtttca
    17941 gtctgatgct gggctgcatg ttgtgtacct ctcgtgccat tagcgccgct ttaccgctgg
    18001 tgccgcaggt cagtttcgcc gatcttgacg gaccgacctg gctggcggta gatgtggaac
    18061 cagcgcttca gttcacgacg ggcgaattgc atctttagga tgccagcgca gcaagtttgc
    18121 catcgcaaag agatattttt cgctggcttc gtcggaggag atgggcggaa actctgcggt
    18181 gatgcaatgc aagttgagat ctgcacacca actgccgaaa gagccaggcg tttcataacc
    18241 gacgctggtt accagaggca attcaaacgc ctgggccagc cattcgccta attcgctgtg
    18301 tctgggatct tcaatacaag ccagtggatc gtggaatgag accacccagg caggttgaat
    18361 gcggtgtata agctggcaca gcgccagggt ttcaggttcg gagccaggtt tgtcacctgt
    18421 cagtaatacg acatcacgtt cttcagcggc gctgttccag cgataaactg tttcaccttc
    18481 cttccagttc gccgccggaa agtttctgtt taaatccaca ccattagcat tggcccgtaa
    18541 ccccagctgg cagccgtcag gattcacaca cagcaccaca tgatggcggc gcaaagaagg
    18601 tgtcaaagtc cgcagagcac aggagagcgt gacgaccgaa gagttttcat caccgtgagt
    18661 gccagccaga atcaaaccac tttcgcgact ggctgcaggg gcaggaaacc agattaacgg
    18721 cgcgcccaat aatgaacgtc cgtaatgttc tgttccgggc ggaaatgcgc cgcgttcggc
    18781 gcgtgggcgg gttacggtca tgatcgtctc tggttcagga agtattacag gcagtgttgt
    18841 gcaaattttg cggattatca aatccttttc ttcaacgcta tgtcatcgat cttgtatcac
    18901 ttttttgccg gaagttgttt gcattttatt agttggatag ggtttgtgga tttttgctgg
    18961 ctgacgggca aataaagttt gtttaatggt ttgcttatct gccagttgat taatcacatg
    19021 gcgggctatc tgaccgccag caccaagaat cagtacattt ttcatgaaaa ttatcccaga
    19081 ttattggcag caatatgcca ggccatatca ctattgcgga tttgaatatg tttcattaca
    19141 gagtttcctc aaagaacgga gctaatactg aaattgcttc agccacatag gctttgccgt
    19201 cataaagatc catatggttt gcgccttcaa caatgtgata gcgtttatcc tggcttgatg
    19261 cccgatcgta caggtcgtca ctcatccatt tgctccctgc ctggctgccc gccacaatct
    19321 gcatcggctg agtcaggtac acttccgcca tatggtaagc atcataggta ataatctggt
    19381 taaggctgcg caaagtagcg taacccggtg ctgttggata ctgcgcgcga ggggtgtggt
    19441 aatactccca ggcctgacgc agttcttcgt tcggcgcatc ggactccttc attggtgcca
    19501 gtggcataac ggcatattct ccgctgctaa tatcgctggt tcgggcgttt gaacccgctt
    19561 caacgtatgg cagggcatca atagatttca cattgttttc ccaaccatta cggaacatcg
    19621 aaccaatatt gaccgcacta acggtaccga tggccttgat gcggcgatcc tgaattgcag
    19681 cattggctgt atatcctgca ccggcacaaa ttcccatcgc accaattcgg gtattgtcga
    19741 cataagaaag cgttgtcagg taatcaatca cggcactgat gtcttcagta cgaatgtctg
    19801 ggttttctaa ctgacgcggc tcgccgccac tttcaccttg ataagatgcg tcataagcaa
    19861 tagtgacaaa tcccttttcc gccagttttt tggcataggt tccggccgtt tgttctttaa
    19921 cgcccccacc tgggtgagat accacaattg cctgatactg acgggtttca tcaaattttg
    19981 gagggaaata gatcactgca gacaaagaga tggttggatt attgctgtta gtgaagctga
    20041 ctttattatt catcattccg ttcctcatgg agttgtcggt tcgttttaac ggttggtgat
    20101 atcactatag atattgatca ttaagttgat tagacccaaa atcatgatta gacctatcat
    20161 ttaaatgatt aatagattgc atggagatac tgaatgaagc gtgaagaaat cgctgatctg
    20221 atggcgtttg tcgtcgttgc agaggagcgt agcttcactc gtgcagcagc ccgcctgagc
    20281 atggcgcagt cagctttaag ccagatagtg cgtcgtatag aagaacgatt gggattgcgg
    20341 cttctgacgc gaaccacgcg cagcgttgtt ccaactgaag cgggcgagca tcttttgtct
    20401 gttcttggcc ctatgttgca tgacatagat tcagccctgg catccctgag cgagctgcag
    20461 aaccgcccat ccgggacaat acgtattact actgtagaac atgcagcaaa aacgatattg
    20521 ttaccagcaa tgcgcacatt cctgaaatcg catcctgaaa ttgatattca gctcaccatt
    20581 gattatggtt tgaccgatgt cgtttctgaa cgttttgatg caggcgtccg tctgggtggg
    20641 gagatggata aagatatgat cgccattcga atcgggccag atataccaat ggctattgtt
    20701 ggctcaccgg attatttttc tcgccgaagt gttccaacgt cagtgtcaca attaatagat
    20761 catcaggcaa ttaatttgta tcttcccaca tcgggtacag caaatcgctg gagattaata
    20821 cgcggtggac gtgaagttcg tgttcgcatg gaaggtcagc ttttactgaa tacgatagac
    20881 ctgatcattg atgctgcaat tgatgggcat ggattggcgt atctacctta tgatcaggtt
    20941 gagcgggcta ttaaagaaaa aaaactgata cgtgttttgg ataaattcac accagattta
    21001 cccggttatc acctgtacta tccacaccgt cgacatgctg gctcggcatt ctcattattt
    21061 atagataggc tgaagtataa aggtgctgtt tagcactact tgctgataca ttaatttaat
    21121 ttctctct
//
LOCUS       DAFAVF010000068        20734 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFAVF010000068 DAFAVF010000000
VERSION     DAFAVF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.068"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            251..2644
                     /gene="malP"
                     /locus_tag="LGK42_002513"
     CDS             251..2644
                     /gene="malP"
                     /locus_tag="LGK42_002513"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBK2658752.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2654..4738
                     /gene="malQ"
                     /locus_tag="LGK42_002514"
     CDS             2654..4738
                     /gene="malQ"
                     /locus_tag="LGK42_002514"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBK2658753.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4783..6099)
                     /gene="gntT"
                     /locus_tag="LGK42_002515"
     CDS             complement(4783..6099)
                     /gene="gntT"
                     /locus_tag="LGK42_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBK2658754.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6460..7035)
                     /gene="nfuA"
                     /locus_tag="LGK42_002516"
     CDS             complement(6460..7035)
                     /gene="nfuA"
                     /locus_tag="LGK42_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBK2658755.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7094..7777)
                     /gene="gntX"
                     /locus_tag="LGK42_002517"
     CDS             complement(7094..7777)
                     /gene="gntX"
                     /locus_tag="LGK42_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBK2658756.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7815..8585
                     /gene="bioH"
                     /locus_tag="LGK42_002518"
     CDS             7815..8585
                     /gene="bioH"
                     /locus_tag="LGK42_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBK2658757.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8614..9492)
                     /gene="rpnA"
                     /locus_tag="LGK42_002519"
     CDS             complement(8614..9492)
                     /gene="rpnA"
                     /locus_tag="LGK42_002519"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBK2658758.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(9695..9931)
                     /gene="feoC"
                     /locus_tag="LGK42_002520"
     CDS             complement(9695..9931)
                     /gene="feoC"
                     /locus_tag="LGK42_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBK2658759.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(9931..12252)
                     /gene="feoB"
                     /locus_tag="LGK42_002521"
     CDS             complement(9931..12252)
                     /gene="feoB"
                     /locus_tag="LGK42_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBK2658760.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12269..12496)
                     /gene="feoA"
                     /locus_tag="LGK42_002522"
     CDS             complement(12269..12496)
                     /gene="feoA"
                     /locus_tag="LGK42_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBK2658761.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(12934..15255)
                     /locus_tag="LGK42_002523"
     CDS             complement(12934..15255)
                     /locus_tag="LGK42_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK2658762.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15352..15828)
                     /gene="greB"
                     /locus_tag="LGK42_002524"
     CDS             complement(15352..15828)
                     /gene="greB"
                     /locus_tag="LGK42_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBK2658763.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16056..16775
                     /gene="ompR"
                     /locus_tag="LGK42_002525"
     CDS             16056..16775
                     /gene="ompR"
                     /locus_tag="LGK42_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBK2658764.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16772..18124
                     /gene="envZ"
                     /locus_tag="LGK42_002526"
     CDS             16772..18124
                     /gene="envZ"
                     /locus_tag="LGK42_002526"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBK2658765.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18156..18452)
                     /locus_tag="LGK42_002527"
     CDS             complement(18156..18452)
                     /locus_tag="LGK42_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2658766.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(18511..18828)
                     /locus_tag="LGK42_002528"
     CDS             complement(18511..18828)
                     /locus_tag="LGK42_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2658767.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            complement(18945..20567)
                     /gene="pckA"
                     /locus_tag="LGK42_002529"
     CDS             complement(18945..20567)
                     /gene="pckA"
                     /locus_tag="LGK42_002529"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK2658768.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
BASE COUNT         4910 a         5295 c         5640 g         4889 t
ORIGIN      
        1 ttaccttccg ctttttgaaa atacgcaacg gccatttttt gcacttagat acagattttc
       61 tgcgctgtat tgcattgatt tgctgctaat cctgtggttt gcactagctt taagtggttg
      121 agatcacatt tccttgctca tccccgcaac tcctccctgc ctaatccccc gcaggatgag
      181 gaaggtcaac atcgagcctg gcaaactagc gataacgttg tgttgaaaat ctaagaaaag
      241 tggaactcct atgtcacaac ctatttttaa cgataagcaa tttcaggaag cgctttcacg
      301 tcagtggcag cgttatggct taaattctgc ggctgaaatg actcctcgcc agtggtggct
      361 agcagtgagt gaagcactgg ccgaaatgct gcgtgctcag ccattcgcca agccggtggc
      421 gaatcagcga catgttaact acatctcaat ggagtttttg attggtcgcc tgacgggcaa
      481 caacctgttg aatctcggct ggtatcagga tgtacaggat tcgttgaagg cttatgacat
      541 caatctgacg gacctgctgg aagaagagat cgacccggcg ctgggtaacg gtggtctggg
      601 acgtctggcg gcgtgcttcc tcgactcaat ggcaactgtc ggtcagtctg cgacgggtta
      661 cggtctgaac tatcaatatg gtttgttccg ccagtctttt gtcgatggca aacaggttga
      721 agcgccggat gactggcatc gcagtaacta cccgtggttc cgccacaacg aagcactgga
      781 tgtgcaggta gggattggcg gtaaagtgac gaaagacgga cgctgggagc cggagtttac
      841 cattaccggt caagcgtggg atctccccgt tgtcggctat cgtaatggcg tggcgcagcc
      901 gctgcgtctg tggcaggcga cgcacgcgca tccgtttgat ctgactaaat ttaacgacgg
      961 tgatttcctg cgtgccgaac agcagggcat caatgcggaa aaactgacca aagttctcta
     1021 tccaaacgac aaccatactg ccggtaaaaa gctgcgcttg atgcagcagt acttccagtg
     1081 tgcctgttcg gtagcggata ttttgcgtcg ccatcatctg gcggggcgta aactgcacga
     1141 actggcggat tacgaagtta ttcagctgaa cgatacccac ccaactatcg cgattccaga
     1201 actgctgcgc gtgctgatcg atgagcacca gatgagctgg gatgacgcct gggccattac
     1261 cagcaaaact ttcgcttaca ccaaccatac cctgatgcca gaagcgctgg aacgctggga
     1321 tgtgaaactg gtgaaaggct tactgccgcg ccatatgcag attattaacg aaattaatac
     1381 tcgctttaaa acgctggtgg agaaaacctg gccgggcgat gaaaaagtgt gggccaaact
     1441 ggcggtggtg cacgataaac aagtgcatat ggcgaacctg tgtgtggttg gcggtttcgc
     1501 ggtgaacggt gttgcggcgc tgcactcgga tctggtggtg aaagatctgt tcccggaata
     1561 tcaccagcta tggccgaaca aattccataa cgtcaccaac ggtattaccc cacgtcgctg
     1621 gatcaaacag tgcaaccctg cactggcggc tctgttggat aaatcactgc aaaaagagtg
     1681 ggctaacgat ctcgatcagc tgatcaatct ggaaaaattc gctgatgatg cgaaattccg
     1741 tcagcaatat cgcgagatca agcaggcgaa taaagtccgt ctggcggagt ttgtgaaagt
     1801 tcgtaccggt attgagatca atccacaggc gattttcgat attcagatca aacgtctgca
     1861 tgagtacaaa cgccagcacc tgaatctgct gcatattctg gcgttgtaca aagaaattcg
     1921 tgaaaacccg caggctgatc gcgtaccgcg cgtcttcctc ttcggcgcga aagcggcacc
     1981 gggctactac ctggcgaaga atattatctt tgcgatcaac aaagtggctg acgtgatcaa
     2041 caacgatcca ctggttggcg ataagttgaa ggtggtgttc ctgccggatt attgcgtttc
     2101 ggcggcggaa aaactgatcc cggcggcgga tatctccgaa caaatttcga ctgcaggtaa
     2161 agaagcttcc ggtaccggca atatgaaact ggcgctcaat ggtgcgctta ctgtcggtac
     2221 gctggatggt gcgaacgttg aaatcgccga gaaagtcggt gaagaaaata tctttatttt
     2281 tggtcatacc gtggaacaag tgaaggctat tctggccaaa ggctacgacc cggtgaaatg
     2341 gcggaagaaa gataaggtgc tggacgcagt attgaaagag ctggaaagcg gtaaatacag
     2401 cgacggcgat aagcatgcct tcgaccagat gctgcacagt atcggcaaac aaggcggcga
     2461 tccgtatctg gtgatggcgg atttcgcagc ctatgttgag gcacaaaagc aggtggatgt
     2521 gctgtaccgc gaccaggagg cctggactcg cgcggcgatc ctcaataccg cccgctgcgg
     2581 tatgtttagc tcggatcgct ctattcgcga ttatcaggct cgtatctggc aggcaaaacg
     2641 ctaaggaagc tcgatggaaa gcaaacgtct ggataatgcc gcgctggcgg cggggattag
     2701 ccccaattac atcaatgccc acggtaaacc gcagtcgatt agcgccgaaa ccaaacggcg
     2761 tttgcttgac gcgatgcatc aacgtaccgc cacgaaagtg gcggtaacgc cagtcccgaa
     2821 tgtcatggtt tataccagcg gcaaaaaaat gccgatggtg gtggagggca gcggcgaata
     2881 tagctggctg ctgaccaccg aagaaggaac acagtacaaa ggccatgtaa cggggggcaa
     2941 agcgttcaat ctaccgacga agctgccgga aggttatcac acgctgacac tcacccagga
     3001 cgaccagcgc gcgcattgcc gggtgattgt cgccccgaaa cgctgttacg aaccgcaggc
     3061 gttgctgaat aaacaaaagc tgtggggtgc ctgcgttcag ctttatacgc tgcgatcgga
     3121 aaaaaactgg ggtattgggg attttggcga tctcaaagcg atgctggtgg atgtggcaaa
     3181 acgtggcggg tcgttcatcg gcctgaaccc gattcatgcg ctctatccgg caaatccgga
     3241 gagtgccagc ccatacagcc cgtcttctcg ccgttggctg aatgtgattt atatcgacgt
     3301 taacgccgtt gaagatttcc atcttagcga agaggctcag gcctggtggc agttgccgac
     3361 cacgcaacag acgctgcaac aggcgcgcga tgccgactgg gtcgattact ccacggttac
     3421 cgccctaaaa atgacagcat tacgaatggc gtggaaaggt ttcgcgcaac gtgatgatga
     3481 gcagatgacc gcgtttcgcc agtttgttgc agagcagggc gacagcctgt tctggcaggc
     3541 agcctttgat gcgctacatg cccagcaagt gaaagaggac gaaatgcgct ggggctggcc
     3601 tgcatggcca gagatgtatc agaacgtgga ttcaccagaa gtgcgtcagt tctgcgaaga
     3661 acatcgtaat gacgtcgatt tttatctctg gttgcagtgg ctggcttaca gccagtttgc
     3721 cgcctgctgg gagataagcc agggctatga aatgccgatt ggcttgtatc gtgatctggc
     3781 ggttggcgta gcggaaggtg gggcggaaac ctggtgtgac cgtgaactat attgcctgaa
     3841 agcgtcggtt ggcgcgccgc cggatatcct cggcccgttg gggcagaact ggggattacc
     3901 gccaatggac ccgcatatca tcaccgcgcg tgcctatgaa ccgtttatcg agctgttgcg
     3961 tgccaatatg caaaactgcg gcgcattacg aattgaccat gtgatgtcga tgctgcgttt
     4021 gtggtggata ccgtatggcg agacggcaga tcagggcgcg tatgttcact atccggtgga
     4081 tgatctgctc tcgattctgg cactcgaaag taaacgtcat cgctgtatgg tgattggtga
     4141 agatctcggt accgtaccgg tagagattgt cggtaagctg cgcagcagcg gtgtgtactc
     4201 ctacaaagtg ctctatttcg aaaacgacca cgagaagacg ttccgtgcac cgaaagcgta
     4261 tccggagcag tcgatggcgg ttgcggcgac acatgacctg cctacgctac gcggttactg
     4321 ggagagcggg gatctaacgc tgggcaaaac cctggggctg tatccggatg aagtggtact
     4381 gcgcggtctg tatcaggatc gcgaactggc gaagcaaggg ctgctggatg cactgcataa
     4441 atatggttgt ctgccaaaac gtgccgggca taaggcatcg ttgatgtcga tgacgccgac
     4501 gctgaaccgt ggtttgcagc gctacattgc cgacagtaac agtgctctgt taggactaca
     4561 gccggaagac tggctggata tggccgaacc ggtgaatatt cctggcacca gttaccagta
     4621 taaaaactgg cgacgcaagc tttccgcaac gcttgagacg atgtttgccg atgatggcgt
     4681 gaacaagttg ctgaaggatt tggacagacg gcgcagagct gcagcgaaga agaagtagag
     4741 tgcggttgat gccggatgcg atgcatccgg cagtcagtgt tatcaaatca ccatattcag
     4801 cagcagacag cctaccagac cgcacaccga gataatggtt tccagcatcg accaggattt
     4861 gatggtctcg ccgatagtca ggttaaagta ctctttgaac agccagaagc ccggatcgtt
     4921 cacatgagag aaaatcacac taccggaacc caccgcaata accatcagct ctgggctaac
     4981 acccgtcgtt gcaatcagcg gtgccgcgat accacccgca gtgattgccg caacggttgc
     5041 ggaacccagc gcgatacgca gtacggcagc aatcgaccag gccatcagca gcggagaaat
     5101 gttggtttcg tgcatcatgg aagcaatgta tttgtccacg ccgctgtcta ccagcacctg
     5161 cttgaacgca ccgccaccac cgatgatcaa cagcatcatc gcaatgattt tgatggaaga
     5221 aaccagcgtg tcgttaatct gatccattga acgaccacgg ttcagaccaa aggtgaacat
     5281 cgcaatcagc acggcaatca gcgttgccat taccgggtca ccgaggaact ccgctaccgg
     5341 caggaaagcg tgacctttcg gcaggatcat ttcggcaatc gcacgcatcg ccatcagcac
     5401 aaccggcacc agagaagtcc agacgctgac gccaaagctc ggcatctctt cttcgctgaa
     5461 ggttttcgcg ctgtagagac cttccgggat tggcttatcg atacctttca acacgcgagc
     5521 gtaaaccgga ccggcgagaa tcacggtcgg gattgccaga atagtaccgt acagcagggt
     5581 tttacccata tcggcattga aaatggtggc aatcgcagtc gggcccggat gcggtggcag
     5641 gaagccgtgg gtcacagaca gtgcagccgc cattggtaca ccaacataca gcagtggaat
     5701 attcgcggaa gccgcgatgg taaacaccag aggcagcatc agcacgaagc ccacttcata
     5761 gaacagggca aaaccaacgg taaaaccggt taacaccacc gcccactgga tgtgcttttt
     5821 accaaatttg gcaatcagcg tggtggcgat acgttgtgcg ccaccgcagt ctgccagcat
     5881 tttgcccagc attgcgccaa aacccatgat cagggcaagg ctaccgagcg tcccgccgac
     5941 accggctttg atggagccaa taactttatc cagcggcatt ccttgcatta atccaacagc
     6001 aagcgccacg aggacgagag cgatgaagcc gttcattttg aagcggatca tcaggagcaa
     6061 caacaagatt acaccgatag caacaatgac taatggcatg atttacctgg cctttcattt
     6121 gttatgggta acgtcaattt tctgacgaca aactctaatt atcccaatcg ggaacagaga
     6181 tattgcggca ccacgactga tacccactaa aactaattat tgtagtcaga tgtcaggagt
     6241 atgtttggta cccatgtgaa tgatacgggt aacatctggc gtttgagaat caccagagcg
     6301 gggtaaattt aaattatgag aggttggtca tattatcgcg gggaaacgaa gggagggatt
     6361 tgccaaagca atgctgtgcc aacgtctggc acatgttcaa cgtaggcccg aaatgacgct
     6421 ttagcgtcgc atcgggcaat ctacaaaaga ggggataact tagtagtagg agtgttcgcc
     6481 gcgctggtgt tcggtgagat cgcgcacacc tttcagctcc gggaattcgt tcagcagctg
     6541 cttctcgatc ccttctttca gcgtcacatc gaccatggaa caaccgttac agccgccgcc
     6601 aaattgcaga atggcgtaac cgtcttctgt gatttccatc agcgaaacgc gaccaccgtg
     6661 accagcaagc tgtgggttga tctgcgactg cagcatatac tccacgcgct ccatcagcgg
     6721 tgcatcgtct gccactttac gcattttggc gttcggggct ttcagcgtta actgagaacc
     6781 caactggtcg gtaacaaagt cgatctctgc atcttccagg tatggtgcgc ttaactcatc
     6841 aacatacgcg gtcagcaggt caaatttcag ggcagtgtcg gtggcttcca cggcatccgg
     6901 cggacaataa gaaacaccac attcagcgtt aggcgtgcca gggttaatca caaatacgcg
     6961 gatttgtgtc ccttcttcct gatttgccag cagtttggca aagtgcgctt gtgcagcatc
     7021 ggaaatacgg atcatagtaa tggcctaata gttgactatt ttagttggtt ataatacgcc
     7081 catcatcgag gctctacaag gttcgacaaa ggcaccagac ctggacagtc gccgcaccat
     7141 tgcgtaaaag caactgcgca atctctgcga cggtacttcc ggtggtaacg acatcatcca
     7201 caatcaccat atggcgacct tgcacgggca attcaagacg aaaggcattt ttcaggttgc
     7261 gcttgcgcag ccgggcactg agaaaatgct gggtcgcagt ggcccgtgta cgtgtgacgg
     7321 cttcgctatc ccattggcag tgcaaccagc gtgataacgg ctgacacagc aaatcgctct
     7381 gattaaatcc ccgacgccag tgacgccgct gccataacgg aacgctgatg atgcgatccg
     7441 gcaattgcaa cccggtggtg cgacgagcgt gcaagacttc caatagtaac agacgtgaca
     7501 gggcgctggc gatttcactg cgccgggaaa atttaagctg gtggatgagc agacttaacg
     7561 gcggcgcata gtcggcaact gtgaccagtc tttgccaggg cggcggtttt tgcaggcagc
     7621 gaccgcaggg aagatgggag tgtgtggcgg gtaatccaca ttgtgggcat aacgttttat
     7681 ctgtgcgggt ggcgcgtgaa cagaccgaac aaatccccca atgacctaac gccagtggca
     7741 ttcggcatag ccagcataat cccggtactg ttagcatatg ttcatccttg taagtcaaaa
     7801 gagaacaata gcggatgaat aacatctggt ggcagaccaa aggtcagggg aatgttcatc
     7861 ttgtgctgct gcacggatgg ggactgaatg ccgaagtgtg gcgttgcatt gacgaggaac
     7921 ttagctcgca ttttacgctg caccttgttg acctgcccgg cttcgggcgt agccggggat
     7981 ttggtgcgct gtcacttgct gatatggccg aagccgtgct gcaacaggca cctgataaag
     8041 ccatttggtt aggctggagt ctgggcgggc tggtggcaag ccagattgcg ttaacccatc
     8101 ccgagcgtgt tcaggcgctg gtcaccgtgg cgtcgtcacc ttgttttagt gctcgtgacg
     8161 agtggccggg gataaaaccg gacgtgctgg cgggatttca gcagcaactc agtgatgatt
     8221 ttcagcgtac agtggagcgg ttcctggcgt tacaaaccat gggcactgaa acggcgcgcc
     8281 aggatgcgcg ggcgttgaag aaaaccgttc tggcgttacc gatgccggag gttgacgtgc
     8341 ttaatggcgg gctggaaatc ctgaaaacgg tcgatctccg tcagccgctg caaaacgtgc
     8401 ccatgccgtt tttgcgattg tatggctatc tcgacggtct ggtgccgcgc aaagtggtgc
     8461 cgatgctgga taaactttgg cctcacagcg aatcatatat cttcgccaaa gcggcccatg
     8521 cgccgtttat ttcgcatccg gtcgagtttt gtcacctgct ggtggcgttg aagcagaggg
     8581 tgtaggcaac ttttgaaatg gcgagacaga aatttactgg ctcgccgcag ccaactcttc
     8641 ttctgacacc ccggtaaagc gcatgatgtc tgtaagaggg actccggatt caagcattat
     8701 tttggctata tgcagggctt tggattgttc cccctcctgc cgcaatcttt ccgcaattgt
     8761 cattaaactc tccttgtgtt tcggtgaacg ttcggcaacg ccgtcgataa aatcgttaaa
     8821 acgtaccgcg tcgccggttt gcagtatgta attaaacagc cctttgattt gtctgtcatt
     8881 agcgtatcca ctacttaata agcaggccat ttgctctacc agccccatca ggtcgcgttg
     8941 acgaatatgt ttttgaatta actccagcag cgccatacgt cggtgctgca tgatttcatc
     9001 atcaggcatg acggtgacat caatcagcgg aaatgcggag gcataaagct gccttgccag
     9061 tttgggatcg gcgaaacaat ccagccaaca cagcgaatag ggataggggc tttcaatacc
     9121 gtggtaaaac aacaatagca ccaccatcgg caacgttttg tatccagcat caaggtgatt
     9181 ttgcattgcg gcaatagcgt aacgcatcat gcgaaatgcg atcagtttgt ttgaggtgct
     9241 ttgatgttca atcagacaat agatgtatcc tggtccttgt tccgttttca ccgaccacag
     9301 cacatcggaa tagctttcac gcagatcgtc atcaataaag ctgctcgact ccagcttgag
     9361 ggttttcata tcacagagcg cgtgaatggg ggccggtaaa tggaacgcaa gaaaatcgcg
     9421 agccgtgtcc ggttggcgta aaaagagttt gaacagcgca tcatgtgggg tggaactctg
     9481 ctttttactc atggcgttcc ttcacctgtt gtttgaatgc cgcgactcta cgcgccactc
     9541 cacatctgtg cattagcgag ttttcatccc tgcgagatgc tttcaaagga acttacgccg
     9601 tgctgcaaga gttgcatttt tatggaaagc cgcacgcaaa aaagcccgga tgcagacgca
     9661 taccgggctt tccaaaacag gcgatggagt aaggttaacg cagcgcccac cactcgcgca
     9721 gacaggcttt tccttccggg cagcttttac aactgccaga gaggcagccg tcaggttctt
     9781 cctgaatccg cacggctttg cccatacttt ccagttgttg cagcatggcg ttaatcattg
     9841 gctgtggagt gttcaatgtc tggcttatct gggccgcttc catacggccc cgtaacgcca
     9901 gcaaatcgcg cacctgaata agtgaagcca ttaatggcaa tcaccggtgg tgctggctgc
     9961 gcagcaactg cttaccgact tgcgggttgc cagcagttcg atatccaccc ggctacgcgc
    10021 gcggcgcagc agaccgataa ccacgatgtt aaacaggata accgccagaa tgcacaccag
    10081 gctgtaagtt gggtgctgac tgtagctggc gacctgatag aacaatgttg ccagcgagta
    10141 agcgatattc agcccccaca ggatggagaa gcccatccag ccacggcttg attcacgggc
    10201 gatggctccc atcaccgaga tacatggtac atacagcagg acgaaaatca ggtagctgta
    10261 agctgctgct gcgctaccga atttctgatc catcacgccc atcgccccgg tacccatttc
    10321 gccgtcgcct ttgctggctt caatggggtt catcagtacg ctaaggctga aggtgtcttt
    10381 caggctctgc caggtttcat ctatcgcact gaacagctct tcaccgaggt taaactctgc
    10441 cggattgaac tcttcgtcct gaatattttc tgcggtgtag agggtgttga gcgtacccac
    10501 taccacttct ttcgccatgg cacctgtaaa caggccaacc gttgcctgcc agttatcttc
    10561 atgcacgcca attggcttga agaccggggt gatcacccgg ctgacggacg ccagcgccga
    10621 gtcgttgatg ttatcgacga ttttcccgct cagcgagaag ctgttgaaag cgctcaggaa
    10681 aatgctgacg atgatgatca ctttaccagc acgcagaacg aagcctttca gacgctgcca
    10741 ggtctggata atcaggcttt taacgtgtgg tacatgatag accggcaact ccatgacaaa
    10801 cggcgtcgct tcaccgcgca tgatggtgta cttgagcatc aggccagtca gcaccgccat
    10861 cacaataccc agcatataca gcgagaagac cgccagcgca ccgttctgcc cgaagaaggc
    10921 agccgcgaat actgcgaaga tagccagacg cgcgccgcag gacataaacg gtgccatcat
    10981 gatggtcatc agacgttcac gcggtgcatc aagcgtacgt gcacccatta ccgacggtac
    11041 gttacaaccg aaaccgacga tcagcggcac aaaggatttc cccggcaagc ccagcgcctg
    11101 catcagacgg tccatcacaa acgccgcacg cgccatatac ccggagtcct caaggaagga
    11161 gaggaacagg tacatcatgc caatctgcgg caccagtggc agcacggtgt taatgccgcc
    11221 acccaggccc tgggcgagga agatagtcag ccagtccggg aagtggagcg tgtagccaat
    11281 ccattgaata ccatgcacaa atagcgccac ggagccgacg tcaaacagcg gctgtaacgc
    11341 cccgccgatg ttgatagcca gcaggaacat caggtacatc acaaagagga aaatcggcag
    11401 accgaggaaa cggttgagca cgattttatc taccgcagtg gtgaaacggc tgggttctgc
    11461 cgtcagggtg ttgcttacca catcacagat ggcagcaatg cactggtaac gcgcatcggc
    11521 aatgtgcagc gccggatcgt ccatctcatt acgcagacgg gcgagggcgg catccagatg
    11581 ctgcgacgct tcaccggcgt aggcgcggct gtagatatcg ccttccagca tttgcaggcc
    11641 cagccagcga cgttgtttca gcgggatgtc ggaaggcatc acttttgcca gtgaatctgc
    11701 ttcgttgagt agcggctgtg cgtaatgcac cagttccaca ttctcgttag ctttatagcg
    11761 atcaatcgcc agcttgagcg cttcaatacc gcgaccacgg gttgaaacca gcgggatcac
    11821 tgggcagcca agacgcgccg acagagcatc aatttcaata cgaatatttt gcttctcggc
    11881 aatgtcgagc atgttcagtg ccacgatgca gggaatgccg agttccagca gttgtagcgt
    11941 caggtacagg ttacgctcaa ggttagacgc atccaccacg ttaatcagca ggtcggcgtc
    12001 gccactcaaa atgtagtgac aggcgatttg ctcatcgagc gaggtctgcg atgagatggt
    12061 ggtcagagaa taggtgccgg gcaggtccac cagcgtgacc tgatgatcgg tggtggagaa
    12121 ttgcccttct ttacgttcga cggtaacgcc agcccagtta cctacacgct gacgtgcgcc
    12181 agtgagctgg ttaaataagg ttgtcttgcc agaatttgga ttaccaatta agcctatggt
    12241 taattttttc attgttgtta tcaccgtatt aacaggaaac cgcttccact tctaataagg
    12301 ccagatcttt tttgcgtaat accaggctca cacgacgggt ttcgatatga atggggtcgc
    12361 cgagtggagc gacgcgcacc acattaaaag aggagccagg taacatgcca agagaaagca
    12421 gtttttggcg atatgccggg ctgatttcac gggaaaagcc agtgattttc cacgcagtat
    12481 ctggagtgta ttgcataggt gcctacttgt ttctcattaa ctggataact acctcatggg
    12541 gcgtttgaca gcgacatgag accagatgat ttgccaacaa aggagagatt aaataatcaa
    12601 caacacaatg ataatgagaa tggtttctat cagcaatact taaaatgtgt gcgaatgttg
    12661 gtttgaataa taatctgact caatgttgat gtggctcaag gttatatcgc agcgaaaata
    12721 taattcgaat agcgaattac caggactggc taatgtttaa ttaaggtttc agttgttaat
    12781 gaagtgataa atgttgccgg aatatattgt gagtttggct gtggaataaa aatgccggag
    12841 atattctccg gcacttttat gtcagacaag gcgttcgttg ccagatgcgg cgtggacacc
    12901 ttatcgcgca ggtttagggg tcaatcaggg cgtttaacgt tttttgccca ttgctgccgc
    12961 cagcgcgtcc atcatcgcgc tgttaccggc aggctgcgct tcacgtccgc gcggtttggc
    13021 tgccgggcgg ttgttttgcg ggcgttcatt accgccgccg cgacgggcgt tggtttcgcc
    13081 aggctgctcg tccagacgca tggtcagggc gatacgttta cgctgaagat ccacttccag
    13141 cactttcacc ttcacaatgt cgcccgcttt caccacggta tgcggatctt ctacaaactt
    13201 gttcgacaat gaagagatgt gaaccaggcc gtcctgatgc acgccgatat cgacaaacgc
    13261 gccaaagttg gtgacgttgg tcaccgcacc ttcgaggatc atacccggtt gcaggtcgtt
    13321 cattgtctcg acgccatcgg caaactgagc ggttttaaat tccggacgcg gatcgcgacc
    13381 cggtttttcc agctctttga tgatgtcagt tactgtcggc acaccgaatt tttcatcagt
    13441 aaagtcagac gctttcaggt tacgcagttc gctgctgtta cccatcagat ctttcagtgc
    13501 ctgctgtgtt gctgccagaa tgcgttccac caccggatag gcttccgggt ggacggtaga
    13561 cgcgtccagc gggttatcac catggttaat gcgcaagaag cccgcgcact gctcgaaggc
    13621 tttcggcccc agacggctca ctttcagcag ttgctgacgg ttctggaact gaccgttctc
    13681 atcgcgccag gccacgatgt tttgcgccat catgcgcgtc aggcccgcca cgcgggttaa
    13741 cagcggaaca gaagcagtgt tgagatcgac gccaacggcg tttacgcagt cttctactac
    13801 tgcgtccagt ttgcgggcca gttgcgtctg gctgacgtca tgctgatact gacctacgcc
    13861 gatagatttc ggatcgattt tcaccagctc cgccagcgga tcctgcaaac ggcgggcgat
    13921 agacaccgcg ccacgcagcg aaacgtcgag atccggaaac tcctgcgctg ccagctcgga
    13981 agccgagtaa accgacgcgc cagcttcgct gacgatcact ttctgtgcgg tcactttcgg
    14041 gaactgcttc tgcacgtcga gatagaaacg ttcggtttcg cgggaagctg taccgttgcc
    14101 gatcgccacc agttcaacgt tatgcttttc acacagcgca gcaacagtca tcgctgcttt
    14161 tgcggcttgt ccggtgtgcg ggtaaatggt atcggtcgct accagtttgc cagtggcatc
    14221 gaccaccgcc acttttaccc cagtacgcag acccggatcg aggcccatcg ttgcacgcag
    14281 tccggcaggg gccgccatca gcagatcgtg caggttacgg gcaaagacgt tgattgcttc
    14341 atcttccgcg cgttcgcgca cggtacccat cagttcggtt tccagatgca tcagcacctt
    14401 gatgcgccag gtccagctca ctacgccttt gcgccagcta tccgccgggg cattgttcag
    14461 gcgcaggcca aggtgatcca tgatgatttg ctcgcaatag ctctctttgg gcggctcatc
    14521 gaactgcgga tcggcattca gcgaaagctg gagtacgcct tcgttacgcc cacggaacat
    14581 cgccagcgcg cggtgagaag gcaccgtgga caacggttcg tgatgatcga aatagtcgcg
    14641 gaattttgcc ccttcctctt ctttaccgct caccacggtt gagaccagat gcgcgttctt
    14701 ccacagataa tcacgcactt ttgccagcag cgcggcatct tcggcaaacc gttccatcag
    14761 gatatagcgc gcgccgtcca gcgcggcttt ggtatctgcc acgcctttat cggcatcaac
    14821 atattgtgca gcggcgactt ctggcgtgtg tgacggatcg ctccacagca ggtcagccaa
    14881 cggctcaagc cctgcttcaa tggcgatttg cccgcgggtg cggcgtttag gtttgtaggg
    14941 caggtagagg tcttcgagtt cggttttgct taaggtggcg ttgatggcat tcgccagctc
    15001 gtcagtgagt ttgccttgct cggaaatgga tttgaggatc gcctgacgtc tctcttccag
    15061 ttcgcgcagg tatcccagac gcgtttccag attacgcagc tgcgtgtcat ccagaccgcc
    15121 ggtgatttcc ttacgataac gtgcgataaa cggcacggta ttcccttcgt caagcaggcg
    15181 aacggcagcg tcaacctgtt ccgggcgcgc ctgaatttca cccgcaataa tgcggcagaa
    15241 cgaatcattc atcatggttt gagttcatct tttcggatca aaaatcaggg gatagttata
    15301 cggactggct ggcaaaaatg ccagccatcg gcaggaggtt aagactcttc cttacggttt
    15361 cacgtactcg atagcattaa cataccagct cgcttccccg gcaggggtat tcaccaccgc
    15421 cagatcgccg acttcttttt tcagcaatgc gcgggccatc ggggaatcga tagagatgta
    15481 atctttacgg ccaaaaattt catcgtagcc gacaatacgg aaacggtgag tcacgccatc
    15541 gtcgttttca atctccaccc atgcgccaaa aaagaccttg ccttcctgct gcggggagta
    15601 atcgacgatt ttgagatttt ccatgcattt agtgagatag cgcacgcgac ggtcgatttc
    15661 acgcaggcgc tttttattat actgatagtc agcattttcg ctgcggtcgc ccagacttgc
    15721 ggcccaggtc accttttttg tgacctccgg gcgttcttca cgccagagat aattaagctc
    15781 ttgtttgagt ttttcatacc cttcccgggt aaccaggggc gttttcatct cgttgattcc
    15841 ctttgtctgt ttgataatgc gcacattggg tataacgtga tcatatcaac agaatcaata
    15901 atgtttcgcc gaataaattg tatacttaag ctgctgttta atatgctttg taacaattta
    15961 ggctgaaatt cataccagat ttagctggtg acgaacgtga gcttttttaa gaatacacgc
    16021 ttacaaattg ttgcgaacct ttgggagtac aaacaatgca agagaactac aagattctgg
    16081 tggtcgatga cgacatgcgc ctgcgtgcgc tgctggaacg ttatctcacc gaacaaggct
    16141 tccaggttcg aagcgtcgct aatgcagaac agatggatcg cctgctgact cgtgaatctt
    16201 tccatcttat ggtactggat ttgatgttac ctggtgaaga tggcttgtcg atttgccgac
    16261 gtcttcgtag tcagagcaac ccgatgccga tcattatggt gacggcgaaa ggggaagaag
    16321 tggaccgtat cgttggcctg gagattggcg ctgacgatta cattccaaaa ccgtttaacc
    16381 cgcgtgaact gctggcccgt atccgtgcgg tgctgcgtcg tcaggcgaac gagctaccag
    16441 gcgcaccatc acaggaagag gcggtaattg ctttcggtaa gttcaaactt aacctcggta
    16501 cgcgcgaaat gttccgcgaa gacgagccga tgccgctcac cagcggtgag tttgcggtac
    16561 tgaaggcact ggtcagccat ccgcgtgagc cgctctcccg cgataagctg atgaaccttg
    16621 cccgtggtcg tgaatattcc gcaatggaac gctccatcga cgtgcagatt tcgcgtctgc
    16681 gccgcatggt agaagaagat ccagcgcatc cgcgttacat tcagaccgtt tggggcctgg
    16741 gctacgtctt tgtaccggac ggctctaaag catgaggcga ttgcgcttct cgccacgaag
    16801 ttcatttgcc cgtacgttat tgctcatcgt caccttgctg ttcgccagcc tggtgacaac
    16861 ttatctggtg gtgctgaact tcgcgatttt gccgagcctc cagcagttta ataaagtcct
    16921 cgcgtacgaa gtgcgtatgt tgatgaccga caaactgcaa ctggaggacg gcacgcagtt
    16981 ggttgtgcct cccgctttcc gtcgggagat ctaccgtgag ctggggatct ctctctactc
    17041 caacgaggct gccgaagagg caggtctgcg ttgggcgcaa cactatgaat tcttaagcca
    17101 tcagatggca cagcaactgg gcggcccgac ggaagtgcgc gttgaggtca acaaaagttc
    17161 gcctgtcgtc tggctgaaaa cctggctgtc gcccaatatc tgggtacgcg tgccgctgac
    17221 cgaaattcat cagggcgatt tctctccgct gttccgctat acgctggcga ttatgctatt
    17281 ggcgataggc ggggcgtggc tgtttattcg tatccagaac cgaccgttgg tcgatctcga
    17341 acacgcagcc ttgcaggttg gtaaagggat tattccgccg ccgctgcgtg aatatggcgc
    17401 ttcggaggtg cgttccgtta cccgtgcctt taaccatatg gcggctggtg ttaagcaact
    17461 ggcggatgac cgcacgctgc tgatggcggg ggtaagtcac gacttgcgca cgccgctgac
    17521 gcgtattcgc ctggcgactg agatgatgag cgagcaggat ggctatctgg cagaatcgat
    17581 caataaagat atcgaagagt gcaacgccat cattgagcag tttatcgact acctgcgcac
    17641 cgggcaggag atgccgatgg aaatggcgga tcttaatgca gtactcggtg aggtgattgc
    17701 tgccgaaagt ggctatgagc gggaaattga aaccgcgctt taccccggca gcattgaagt
    17761 gaaaatgcac ccgctgtcga tcaaacgcgc ggtggcgaat atggtggtca acgccgcccg
    17821 ttatggcaat ggctggatca aagtcagcag cggaacggag ccgaatcgcg cctggttcca
    17881 ggtggaagat gacggtccgg gaattgcgcc ggaacaacgt aagcacctgt tccagccgtt
    17941 tgtccgcggc gacagtgcgc gcaccattag cggcacggga ttagggctgg caattgtgca
    18001 gcgtatcgtg gataaccata acgggatgct ggagcttggc accagcgagc ggggcgggct
    18061 ttccattcgc gcctggctgc cagtgccggt aacgcgggcg cagggcacga caaaagaagg
    18121 gtaaataaaa cgggaggcga aggtgtctcc cgtttttaga ttgttttcaa ttgattgata
    18181 atgaaaggat tcgcttgcaa aagcaggagc aattttcgag gtgctccggt aggctgacgg
    18241 cgtttggttt cccagctttt gaccaggtca taacttaccc ctacggcgac agcaaaatct
    18301 tgctgacgta aacccgttgc ttcacggatc gcttttacat cgatctcgct aaaagtatgg
    18361 acgtgctctg gttcaggcgt ttcttcaccc ttttcgatgc gtaccatttc ttctgcgctg
    18421 gcaagaagat cagcaaataa ttcatctttc atctgttttc aatgtctccg ttggcatttc
    18481 gaccatggtg gaatttgtgg agcttcaaca ttaaagcagc ttctctgaca atagtttcaa
    18541 tgcctttctt tgttcttctg ttaaatcatc ctgttcattt ttaggataaa tcaacgccag
    18601 ataaattcgg ccattgcgtg ttacgttgta ataaattatc cgaatgccac cccgtttccc
    18661 cattccagaa cggttccagc gaatttttct aaaactgcct gtgcctgcga taatgtcacc
    18721 ggcagtggga tcttgaatga gtagttcctg aaaagcccgg aactcatcat caggtaacaa
    18781 taactggcgg cgtttgctaa atccttgtag ttcaataaag gtgaacataa tgcatcctgc
    18841 ggtctattga ttattataag tgtaccgtgt acatattgat agctaatcag atataaaaaa
    18901 accccttatc cgtttggata aggggtcaat gagatatcag ctgattacag cttcggacct
    18961 gccgctacca gcgcagcacc cgctggggtg tcagtgtatt tatcgaagtt gtcgataaac
    19021 agtttcgcca gggtttcggc tttttcctgc cactgttccg gggaagcgta ggtgttacgc
    19081 ggatcgagga tcttcgtgtc tacgcccggc agttcggttg ggatcgccag gttaaacatc
    19141 ggcagagtga aagtttctgc gttatccagc gaaccgttga ggatggcgtc gataatagcg
    19201 cgggtatctt taatcgagat acgtttgcca gtgccgttcc agccagtgtt aaccagatac
    19261 gcctgcgcac ctgccgcttg catacgtttt accagcactt ctgcgtactg agtcggatgc
    19321 agcgacagga acgccgcgcc gaagcaagcg gagaaggttg gtgtcggttc ggtgatgcca
    19381 cgctcagtac cggccagttt ggcggtgaag ccagagagga agtggtactg ggtttgatcg
    19441 gcagtcagac gagaaaccgg cggcagcaca ccaaaggcgt ctgcggtcag gaagataact
    19501 ttcgttgcgt gacccgcttt ggaaaccggc ttaacgatgt tatcgatgtg atagatcgga
    19561 taagaaacgc gggtgttctc ggtttttgaa ccatcatcaa agtcgatagt gccatcttca
    19621 cgcacggtga cgttttccag caacgcatca cgacggatag cgttgtagat ttcaggttcc
    19681 gcttctttcg acagcttgat agtttttgcg tagcagccgc cttcgaagtt aaacacgccg
    19741 tcatcgtccc agccgtgttc gtcatcgcca atcaggcgac gtttcgggtc ggtggaaagg
    19801 gtggttttac cggtgccgga aaggccgaag aacaccgcaa catcgccttt ctcaccaacg
    19861 ttggcggagc agtgcataga agcgatacct ttcagcggca gcaggtagtt catcatcgag
    19921 aacatccctt tcttcatttc gccgccgtac caggtgccgc caatcagctg catgcgctcg
    19981 gtcaggttaa acgccacgaa gttttcggag ttcagaccct gttctttcca ctgcgggtta
    20041 gtgcacttcg cgccgttcat aacgataaag tctggtttga aacctgccag ctcttcatcg
    20101 ctcgggcgaa taaacatgtt tttgacaaaa tgcgcctgcc aggccacttc ggtgatgaaa
    20161 cggacggaaa gacgagtatc cgggttcgca ccacagaaag cgtcgacaac gaacagacgt
    20221 ttgccggaaa gctgcctggt caccaggcct ttcagatgct gccaggtttc cggagagaga
    20281 ggtttgttgt cgtttttacc tttgcctttg tctgcccacc agaaagtatc gcgagtggta
    20341 tcgtcacgga cgatatactt atcttttggt gaacgaccgg tgaagatccc ggtatcgacg
    20401 gcaacggcac ccagattagt taacaccccg cgctcataac ctgtcaggct cggatcgagc
    20461 tcttcctgat acagcaggtc gtagcttggg ttgtaaacga tatcatgtac gtcactgata
    20521 ccataagcct cgagttcttg cggggtcaaa ccattgttaa cgcgcatttc actgctcctt
    20581 agccaatatg tattgcctga atagtaaagt ctttttgggg gtgttaaccg cgacaaggct
    20641 catagattta cgtatctgga gaaattcata tttgacaaaa aactccgtga ttcctgtcac
    20701 gaaacggttg ctattatcgc agaaatagca tttt
//
LOCUS       DAFAVF010000069        20071 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFAVF010000069 DAFAVF010000000
VERSION     DAFAVF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.069"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..657
                     /locus_tag="LGK42_002530"
     CDS             <1..657
                     /locus_tag="LGK42_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HBK2658769.1"
                     /translation="VLMAIVGIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTT
                     YCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIA
                     QLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGI
                     TGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL
                     "
     gene            complement(711..3566)
                     /gene="valS"
                     /locus_tag="LGK42_002531"
     CDS             complement(711..3566)
                     /gene="valS"
                     /locus_tag="LGK42_002531"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK2658770.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3566..4009)
                     /gene="holC"
                     /locus_tag="LGK42_002532"
     CDS             complement(3566..4009)
                     /gene="holC"
                     /locus_tag="LGK42_002532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBK2658771.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4363..5874)
                     /gene="pepA"
                     /locus_tag="LGK42_002533"
     CDS             complement(4363..5874)
                     /gene="pepA"
                     /locus_tag="LGK42_002533"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBK2658772.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            6141..7241
                     /gene="lptF"
                     /locus_tag="LGK42_002534"
     CDS             6141..7241
                     /gene="lptF"
                     /locus_tag="LGK42_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBK2658773.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDAVPIRRLRASFSRKGAV"
     gene            7241..8323
                     /gene="lptG"
                     /locus_tag="LGK42_002535"
     CDS             7241..8323
                     /gene="lptG"
                     /locus_tag="LGK42_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBK2658774.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8484..9986)
                     /locus_tag="LGK42_002536"
     CDS             complement(8484..9986)
                     /locus_tag="LGK42_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBK2658775.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(10064..11062)
                     /gene="idnR"
                     /locus_tag="LGK42_002537"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(10064..11062)
                     /gene="idnR"
                     /locus_tag="LGK42_002537"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBK2658776.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(11129..12448)
                     /gene="idnT"
                     /locus_tag="LGK42_002538"
     CDS             complement(11129..12448)
                     /gene="idnT"
                     /locus_tag="LGK42_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBK2658777.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12513..13277)
                     /gene="idnO"
                     /locus_tag="LGK42_002539"
     CDS             complement(12513..13277)
                     /gene="idnO"
                     /locus_tag="LGK42_002539"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK2658778.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13301..14332)
                     /gene="idnD"
                     /locus_tag="LGK42_002540"
     CDS             complement(13301..14332)
                     /gene="idnD"
                     /locus_tag="LGK42_002540"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBK2658779.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14549..15112
                     /gene="idnK"
                     /locus_tag="LGK42_002541"
     CDS             14549..15112
                     /gene="idnK"
                     /locus_tag="LGK42_002541"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK2658780.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQEDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(15116..16135)
                     /gene="ahr"
                     /locus_tag="LGK42_002542"
     CDS             complement(15116..16135)
                     /gene="ahr"
                     /locus_tag="LGK42_002542"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBK2658781.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            16331..16415
                     /locus_tag="LGK42_002543"
     tRNA            16331..16415
                     /locus_tag="LGK42_002543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16365..16367,aa:Leu,seq:caa)
     gene            16615..16911
                     /locus_tag="LGK42_002544"
                     /pseudo
     CDS             16615..16911
                     /locus_tag="LGK42_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(16978..17757)
                     /gene="istB"
                     /locus_tag="LGK42_002545"
     CDS             complement(16978..17757)
                     /gene="istB"
                     /locus_tag="LGK42_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBK2658782.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(17757..18779)
                     /gene="istA"
                     /locus_tag="LGK42_002546"
     CDS             complement(17757..18779)
                     /gene="istA"
                     /locus_tag="LGK42_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBK2658783.1"
                     /translation="MVTFETVMEIKILHKQGMSSRVIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            18865..19842
                     /locus_tag="LGK42_002547"
                     /pseudo
     CDS             18865..19842
                     /locus_tag="LGK42_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         4799 a         5176 c         5037 g         5059 t
ORIGIN      
        1 gttctgatgg caatagttgg tattgttttc attggtattt ttaatggtac tttatatagt
       61 ctggtaatga gttttctctg gagcaatacc agtttcggta tacatcgttt caaggtgaaa
      121 ttagatacta cgtattgtat aaaatatgcc attctcgcat ttttagcttt attacctttt
      181 ctcgctgttg ctggttatat tatcttcgat caaatattaa atgcgtatga tagttctgtg
      241 tatgcaaatg atgatattga gaatttacag caatttatgg aaatgcaacg taaaatgata
      301 atcgcgcagt taatctatta ttttgggatt gctgttagca ccagttattt aacggtgtcg
      361 ttgcgaaatc attttatgag caacctgtca ctgaatgatg ggcgtattcg ttttcgctca
      421 actttaacat accacggtat gctttatcgc atgtgtgcgt tggtggtgat atccgggatt
      481 acgggcggtc tggcttatcc actgctgaaa atatggatga ttgactggca ggcaaaaaat
      541 acgtatttgc tgggcgattt ggatgacctt cctttaatca ataaagaaga acaaccagat
      601 aaaggcttct tagccagtat ttcacgggga attatgcctt ctttaccatt tctgtaagag
      661 ataaaaaagg ccggagcatg ctccggcctt cgttttcatc acggtgttga ttacagcgcg
      721 gcgataacag cctgctgttc aatcagcttc gctttcgctt ccgcatagcc ttccagcttc
      781 tcacgctctt tcgcgatgac cgcttccggt gcgcgggcga caaagccttc attcgccagc
      841 ttgttctcga tgcggctgat ttcgccttcg atcttcgcca cttctttcgc cagacgcgcc
      901 agctcatctt ctttgttgat gaggccagcc atcgggatca gcagctctgc gccgtcgata
      961 atcttcgtaa cggaaaccgg acctttgtca tcggcaggca gcacggtgat gctttccaga
     1021 cgcgccaggg tttgcaggaa gccacggttt tcatttacgc gacgttctgc atccgcgctg
     1081 caaccacgca gcagcagctc cagcggtttg cccggcgcga tgttcatttc tgcacggatg
     1141 ttacgtaccg caacgatcgc ctgcttcagc cactcggtgt cggccagcgc cgcttcatca
     1201 acctgagatg catcgtactg cgggaacggc tgcagcatga tggtgtcggc agtgataccg
     1261 caaagtactt tcacacgctg ccagatggtt tcggtgatga acggaatgat cggatgcgcg
     1321 aggcgcagca gaccttccag tacagtcacc agcgtatggc gagtaccgcg cagttcggct
     1381 tcggttccac cgttcattac cggcttggtc agctccagat accagtcgca gaactggttc
     1441 caggtgaact catacagaat gcctgcagcg atatcgaagc ggaagctgtc cagcgcttcg
     1501 cggtacgctt tgatggtctg gttgaactct gccagaatcc agcggtccgc cagcgacagc
     1561 gtcatttcac caccgttgaa gccgcaatcc tgaccttccg tgttcatcag cacaaagcgg
     1621 ctggcgttcc acagcttgtt acagaagtta cggtaacctt ccaggcgctt catatcccag
     1681 ttgatgtcac gaccggtaga cgccagcgcc gccagggtga agcgcagggc atcggtgccg
     1741 tgcggctcaa tgccgttcgg gaactgtttc tcggtgcgct tacgaatttt gtccgccagc
     1801 tgcggctgca tcatattgcc ggtacgtttt tccagcagtt ctggcagcga aataccgtca
     1861 accatatcca gcgggtcgat aacgttaccc ttggatttgg acatcttctg gccttcgtcg
     1921 tcacgaatca gaccggtcat gtaaacagtg tggaacggca cctgcggttt gccattttca
     1981 tctttgatga agtgcatggt catcatgatc atgcgggcaa tccagaagaa gatgatgtcg
     2041 aagccggata ccatcacgct ggttgggtgg aactgacgca gggcgtcggt gttttccggc
     2101 cagccaaggg tagagaaggt ccacagtgca gaagagaacc aggtgtcgag aacgtcttcg
     2161 tcctgacgca gggcaacgtc ggcaccgagg ttattttcct tgcgcacttc ttcttcgttg
     2221 cggccaacat aaacgttacc tgcttcgtcg taccatgcag ggatacggtg accccaccac
     2281 agctgacgag agatacacca gtcctgaata tcgcgcatcc aggagaagta catgttttcg
     2341 tactgcttcg gtacgaactg aatgtcgccg ttctcaaccg cttcaaccgc cggtttcgcc
     2401 agtacatcgg cacgcacgta ccactggtcg gtcagcattg gttcgataac tacgccgcca
     2461 cggtcgccgt aaggaacggt caggtcgtgc ggtttaattt cttccagcag gccgagtgcg
     2521 tcaaccgcag caacgactgc tttacgtgca gcaaaacgct cgagtttctg gaactctgcc
     2581 gggatttcgc tggaataaac gtcagattcg ttacctttgg tatcgaacac ctgggcgctt
     2641 tcacggatat cgccgtcaaa ggtcaggatg ttgatcatcg gcagcgcgtg acgtttaccc
     2701 acttcatagt cgttaaagtc gtgcgccgga gtgattttca cgcagccggt gcctttttcc
     2761 atgtcggcgt gttcgtcgcc aacgatcgga atacgacggt taaccagcgg tagaatgaca
     2821 tatttgccaa tcaggtcttt gtaacgcgga tcttccgggt taacggccac gccggtatcg
     2881 cccaacaggg tttccggacg ggtagtcgca accaccagat aatctttacc gtctgcggtt
     2941 ttcgcgccgt cagccagcgg atagcggatg tgccacatcg aacctttcga ttcgcggttt
     3001 tccacttcca ggtcagagat agcggtgcgc agtttcggat cccagtttac caggcgtttg
     3061 ccacggtaaa tcaggtcttc tttatacaga cgaacgaaaa cttctttcac cgcattggac
     3121 aggccttcgt ccatcgtgaa gcgttcacgc tcccagtcga cggagttgcc gagacggcgc
     3181 atctgacggg taatcgtgcc gccggattcc gctttccatt cccagatttt gtcgatgaaa
     3241 gcttcgcggc cgtagtcgtg acgggtttta ccttcttctg cggcaatctt gcgctcaacg
     3301 accatctggg tagcgatccc ggcgtggtca gtaccgacct gccacagggt gtttttgccc
     3361 tgcatgcgct gatagcggat catggtatcc atgatggttt gctggaaggc gtgacccata
     3421 tgcaaactgc cggtgacgtt cggcggcggg atcatgatgc agaaactttc ctggctttca
     3481 tcgccattag gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata
     3541 tcttgtgggt tatatgtctt ttccattatt tccaggttgc cgtattcagg ttgaaaccag
     3601 ccacgcggta ggctttatag cgttcgcgcg ccagttgttt cagagaatct tcataaggaa
     3661 cgaagtctac cacttctgtg aaagcggtgg caaaatctgc aaagcttgtt cgcagactaa
     3721 tcaatatatc gcgcgggctg ctgctgcgtt tttgcggcca ggcgatctcc accggcgcac
     3781 cgccacgcgg tccttctccc gctaaattat gcggaacaaa gctttctgcc ggacgcgccc
     3841 acagggcttc atccagccgg taagcctgct tttcatcttc acaggcgatg agcacgcgct
     3901 taccgctgcg ccaacgttct gcggcaattt cacacaccag ttgctcaacg gcgcttaagc
     3961 catcgacggt ggtgtcattg tccagaaggt agaacgtcgc gtttttcata tatggggctt
     4021 cttgtggtgg atttaaatac gcatcaggta tacaccgcag cgcctgatgc gacgctgttg
     4081 tgtcttatca ggcctacgag ttcagtgctg tgtgtaggtc ggataaggcg ctcacgccgc
     4141 atccgacaat aacagcattg cctgatgcga cgctgccgcg tcttatcagg cctacgagtt
     4201 cggtgctgtg taggtcggat aaggcgttca tgccgcatcc gacaataaca gcattgcctg
     4261 atgcgacgct tgcgcgtctt atcaggccta cgagttcagt gctgtgtagg tcggataagg
     4321 cgttcacgcc gcatccggca ataacagcct tgcctgacgc aattactctt cgccgttaaa
     4381 cccagcgcgg ttcaacagga actgtgccag caacgctacc ggacgaccgg ttgcgccttt
     4441 tgctttacca gaacgccagg cggttcctgc aatatccagg tgcgcccagt tgtacttacg
     4501 ggtaaagcgt gacaggaagc aacctgcggt aatcgcccca ccaggacgac cgccaatgtt
     4561 cgccatatcg gcaaaattgg actccagttg ttcctgatac tcgtcaccca gcggtaagcg
     4621 ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca gcggattatg
     4681 gttcgccatc agaccagtaa tatgatgacc cagcgcgatc acgcaggcac cggtcagcgt
     4741 cgccacgtca atcaccgctt ccggctcaaa acgctcaacg taagttaaca cgtcgcacag
     4801 taccaggcgg ccttcagcgt cagtattcag cacttcaacg gtttgaccgg acatggtggt
     4861 taacacatcg cccggacgat aggcacgccc gccaggcatg ttttcgcagc ctgccaacac
     4921 gccgataacg ttaatcggca gttgtagctc cgcgaccata cgcatcacgc cgtaaaccgc
     4981 tgccgcaccg cacatatcgt acttcatctc atccatgcct tctgaaggct tgatcgagat
     5041 accgccggag tcgaaggtta aacctttacc caccagcacg attgggcgtg catcttccga
     5101 cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg ttttgcgaac cctgaccgac
     5161 cgccagatag gaatgcatcc ccagctcttt catctgctgt tcgccgataa cgcgggtgat
     5221 aacattcttg ctgtagctgt cagccagctg gcgcgcttgt gaagcgaggt aagcggcgtt
     5281 acagatattc ggcggcatat tgccgagatc tttcgctgct ttaatcccgg cggcaatcgc
     5341 tagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg gcacgttgaa
     5401 caccatttta cgcagcggac gacgcggttc gctcttgttc gttttcagct gatcgaaact
     5461 gtagagcgtc tcttttgccg tctcgacagc ctgacgcact ttccagtagt tgttacggcc
     5521 tttaacgtgc agttcagtca gaaagcagac cgcttccatt gagccagtat cattcagcgt
     5581 attaatggtt ttctgaatga cttgtttgta ctgacgctca tccagctcac gttctttgcc
     5641 gcaaccaata aggagaattc gctcggaaag tacattcgga acatggtgca gcaacaatgt
     5701 ctgccccggt tttccttcca gttcgccccg acgtagcagg gcgctgatgt acccatcgct
     5761 gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga cgcccacgac
     5821 gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact ccatgcacta
     5881 cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag ctttcgtaac
     5941 tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc attggcacgt
     6001 cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac gatttcgacg
     6061 actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc aaaagacgag
     6121 tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag acgctcaaaa
     6181 gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag ttagtgagga
     6241 tcctcggggc agcggttgac ggcgatattc cggcgaatct ggtgctctcc cttctcgggt
     6301 tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc gggctgctga
     6361 tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc tgcggtctga
     6421 gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt gttcacggca atcgtcgcgg
     6481 cggttaacgt gatgtgggcg ggaccatggt catcgcgtca tcaggatgaa gtgttagcag
     6541 aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag caagcgacta
     6601 atggcagctc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa gatgtgttcc
     6661 tcgcgcaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc gattccggac
     6721 atttaaccca gctgcgcgac ggctcccagg tcgtcactct caatcaggga acgcgcttcg
     6781 aaggcactgc actgttacgt gatttccgca ttacggattt ccaggattat caggcgatca
     6841 ttggtcacca ggcggtggcg ctcgacccga acgataccga ccagatggac atgcgcacat
     6901 tgtggaacac tgacaccgat cgtgcccgcg cagaactgaa ctggcgtatc acgttggtgt
     6961 tcaccgtgtt tatgatggca cttatggttg taccgttgag cgtggttaac ccgcgtcagg
     7021 gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc ctgatccaga
     7081 cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacgctgtgg atgtggaccg
     7141 ttaacctgat ttatctggct ttggcgattg ttctcaacct ttgggacgcc gtgccgatcc
     7201 gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt ttggcgtact
     7261 tgaccgctat atcggtaaaa ctattttcac cactatcatg atgacactgt tcatgctggt
     7321 gtcgctgtcg ggcattatca agtttgtcga tcagctgaaa aaagccgggc aggggagtta
     7381 cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg tgcaaatctt
     7441 cttcccgatg gcggctctgc ttggggcgtt gcttggtctt gggatgctgg cgcagcgcag
     7501 cgaactggta gtcatgcagg cttctggttt tactcgtatg caggtggcgc tgtcggtgat
     7561 gaaaaccgcc attccgctgg tcttgctgac gatggcgatt ggtgaatggg tcgcgccgca
     7621 gggcgagcag atggcgcgta actaccgtgc gcaggcgatg tacggcggct cgttgctctc
     7681 tacccagcaa ggtttatggg cgaaagatgg caacaacttc gtctacattg agcgggttaa
     7741 aggtgacgaa gagttaggtg gcatcagtat ctatgccttt aacgagaatc gtcgtctgca
     7801 atccgtacgc tatgccgcta ccgcgaagtt tgaccctgaa cataaagtct ggcgtctgtc
     7861 gcaggttgat gaatctgatt tgaccaatcc gaaacagatc accggttcgc agacggtgag
     7921 cggcacctgg aaaaccaacc tcacgccaga caaactgggc gtggtggcgc tggacccgga
     7981 tgcactctct atcagcggtt tgcacaacta tgtgaagtat ctgaagtcga gcggtcagga
     8041 tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat ccgtggcggt
     8101 gatgatgctg atggcgctgt cgttcatctt tggcccactg cgtagcgtac cgatgggcgt
     8161 gcgtgtggtc actggtatta gcttcggttt tgtcttctac gtactggacc agatcttcgg
     8221 cccgctgacg ttggtttacg gcatcccgcc gatcatcggc gcacttttgc caagtgccag
     8281 cttcttctta atcagcctgt ggctgttaat gagaaaatcg taacctgatg aacgaataaa
     8341 gggagcgaaa gctcccttta ttattgttag caaagtgtgc ttcgttcatg cctgaaaaat
     8401 catgaaaatt caatatattg cagtggtgtg ggcatgataa ggcgcgccag cgtcgcatca
     8461 ggcaaagaca aatcgcacct ggcttacctt cttctccccc ccagcaaact ccccaacatc
     8521 ccgcgcacaa tctgattcgt cacctggcgg gcggcgcttt ttgccattgt ctgcaccaca
     8581 ccatctttct tcccgccgcg tggtccagta gtgccaaaca aaatatcctt caatccaccg
     8641 agaataccgt catccaccgc cacctctttc cctttcgcgg gaggattatt ttgctgctcg
     8701 gtactggcct gaaagccttt ttgcaacatc tcataggcgg attcacggtc cacgtcatcc
     8761 tcgtacttgc catacaccgg ggagtgatta atcaagccgt tacgctcatc ttccgtcacc
     8821 ggccccattc gcgaacaagg cgcgataacc atcgcccgtt ccaccactga aggacttcct
     8881 ttcgcatcga gaaacgagat taacgcctca ccggtcccca gttcctggat tgccttttcg
     8941 gtatcaaacg ccggattggc ccgcatggtt tgcgccgcag cttttactgc tttctgatct
     9001 ttgggcgtaa aagcccgcaa agcgtgttga acgcgattac ctagctgccc gagcacatta
     9061 tccggaatat ctgacgggtt ttgcgaaacg aaccagacgc ccacgccttt tgagcgaata
     9121 agccttatca cctgctcaat cttatccagc agtacctgtg gcgcatcgtt aaacagcaga
     9181 tgtgcttcgt cgaagaaaaa caccagtttt ggtttctcca gatcgcccgc ttcaggcaat
     9241 tgttcataca actctgaaag catccacagc aggctggcgg cgtacagttt cggcatctga
     9301 taaagcttct cggcgctgag gatattgata acgcctttac cgttggcatc ggtacgcatc
     9361 cagtctttga tatccagcat cggctcacca aagaagtgtg ctgcgccttg ctgttccagc
     9421 gataacagcc cgcgctggat ggcaccaacc gatgcgctac tgatattacc gtactgattc
     9481 tggaaggatt tggcgttatc gccgatgtac tgggtaattg cccgcagatc tttaaagtcg
     9541 agcagtaaca gcccctgatc gtcagcaatg cggaaaatga tattcagcac gccagattgc
     9601 acatcgttga gattcaacag ccgcgccagc aacagcggcc ccagatccga aaccgtcgcc
     9661 cgcaccggat ggcccttctc gccaaagata tcccacacca ccaccggatt agcatgcggt
     9721 tgccagtcat ttacgccgat atttttaagc cttgcgagca gtttttccga cgccgttcct
     9781 gcctgcgcga tgccggtcag atcgcctttc acatcggcca taaacaccgg aacaccgatt
     9841 tccgatagag actccgccag tttttgcagc gtgacagttt tacccgtccc cgttgcgcca
     9901 gtaatcagcc cgtgacggtt agccattccc ggcagtaaaa atagttctgt gtccggcgtg
     9961 cgggcaatta acaggggttc gctcatgaga tatcctccag tttcctgcct ggagtatagg
    10021 caacgtccgg caaagagaat acgtgtaatg aagagaaaat aacctaaagc gtgttgccgt
    10081 gataaatctg atacccgagg tcaacagtgt tgtggttgtg atcgttattt ttaattttgc
    10141 tcagcaacat ttgggcggcc atccgaccaa tatcaaaacg gggagtaatt acgctggcga
    10201 ggcttgggat catttgcctg ccaatctcca gcccatgaaa acccgcaatg gaaatttgtt
    10261 ctggcacggc gaggtttcgc tcgcggcaca gcagaagcgc acccatcgca atatcgtcat
    10321 tggtgcaaaa tacaccgtct aaatccgggt ttgcgctaag ggcatcacgc attaactgca
    10381 ttcctaaatg aatggatgag atggcgcgtg gattcatgcg taatggggaa agattatgca
    10441 gcatcatcgc atcgcaatac ccctgataac gctgttcatc gcgggtgtca tctttggaac
    10501 cgagatacag aattttgtgc ctgactcgct tctccagcat ggtacacacc atgtcaaaag
    10561 cggcctgccg attatcaaaa ccgacctcca tatccagccg ttctccctgt acatccatca
    10621 attccacgac cggaatggtg gctgaacgca gaaatttcac cgtcctgatg gtgtgatatt
    10681 tttccgaaag tataatcccg tcaatgttat aagagagcag gttaatcacc gactcttctt
    10741 cggaatcgcg atcgtaattg taattagcaa taagggtctg ataattatgc tcagaggtga
    10801 cggattctat tcccgcgaga atgtcagcga agagttgatt ttgaaatgac ggaatcaaga
    10861 taccgagggt ataactttgt gcattcaaca acatgccggg agcgcgatta gggatataat
    10921 tgatctcttc cataattttg gcgatgcgct cgcctgtttc ctttgccacc tttttcggcg
    10981 agcggatata acgactcacg gtcatttttg ttacgccagc cagcgtagcg atatcctgta
    11041 aagaaattct gtgattgcgc ataacttctc ccaacgtcgg gggaatcttc aatcttcccc
    11101 cgaaactaca tcacaatatt ttattctttc agtgcaatac ggcgttaatg gctaacacgc
    11161 ccagcaaacc cataatagaa ataagggttt ccatcaccgt ccaggtacgc aacgtttcac
    11221 caaccgtcag attaaaatac cctttaaata gccagaagcc agggtcgttt acgtgtgacg
    11281 cgatcacgct gcccgcaccg gttgccagta ccattaaagc gggatcggca tgggtaacgt
    11341 tgataatcgg caacaccaca cccgcggtgg taatggccgc gacggtggca gagcccagag
    11401 cgatacgcaa cagcgccgca acagtccagc acatcaataa cggcgaaagc gtagttccgg
    11461 tcattaagtg tgaaatatag tgcccgacac cgctatctac taatacctgc ttaaacgcgc
    11521 cgccgccagc gataataaac acaatcatcg cgatagcgcc tatagaatcc ccaatgatat
    11581 ccatgatttg ctcgatggtg cgtccattac gtcggcccag tgtgaaaatc gcaataacaa
    11641 tggcaataaa cagcgcaacg gcagggttac cgacaaattc aaagaagagg cgcacggtgt
    11701 tagttttcgg taacgtaatt tcacaaacgg cggcgatagc catcaggatg accgggatca
    11761 cggcagcgaa aatactgttc cagaaggagg gcatctcctc ttcgctaaac agatgaggat
    11821 taaataagcc ttccggtggt gctttctcaa agcgagttag cagtttagaa aacagcggtc
    11881 ctgcgacaat aactgtcgga atggtaatga taaatccata tagtaaagtc gttccgagat
    11941 tagcctcaaa gatagtcgcg atggcagtag gccctggatg tggcggcaga aaacagtggg
    12001 ttacagagag cgctgctacc attggtacgc caacatacaa caggggtaat cctgatgatg
    12061 ctacgatggt aaataccaac ggcaacagca ggacaaaacc cacttcaaaa aacatggcga
    12121 ggcccacaac cagaccagtg atcactagcg cccattgcac gcgtttttta ccaaaagtag
    12181 caatcagcgt agtggcgata cgttgtgccg cacccgtatc agaaatcagc ttgcctaaca
    12241 tggccccgaa accgaggatc attgccagcc caccgagcgt gctgccgata ccattttgta
    12301 tagagtgcag gacggcctgt gcatccatcc cttcggcaaa tccgacgacg gcagctacca
    12361 gaacgagggc aataaagccg ttaactttaa agccgatcat caggataaga agcagcgcga
    12421 cgcctgccgc aataatgatt aatggcatag tctttatctc ttattagtgc cagaaaagca
    12481 gcgctttcgc gctgcacgta gggtagtagg tgttaaacag ccactaacat gccgccatca
    12541 acaaacaaca ggtggccgtt tacgaaatca gaggcttttg aagaaaggaa caccgcagca
    12601 ccaatcagtt cctgcggatc tccccagcgt gcggcggggg tccgtttgca caaccaggcg
    12661 gtgaaggctt cgtcctcaac cagtgcttta gtcatttctg ttttgaaata gcccggcgca
    12721 ataccgttga cctgaatatt gtggcgcgcc agctcgacgc acatgccgcg ggtgagcatt
    12781 tttaccgccc ctttcgatgc ggcataaggg gtgatggtgt cacgtcccag ttcgctttgc
    12841 atcgagcaaa tattaataac tttacctgcc ttgcgttcaa ccatgtgacg agttactgct
    12901 tgcgatacca ggaacacggc ggtctggttt actgcgatca catcattcca ctcttgttca
    12961 gggaactcag taaaaggatg acggcgctgg ataccggcgt tattcaccag cacatcaatg
    13021 gggccgatgt ccttttcgat atgttcaacg gcggcatcaa tttcatgttt atgagtaaca
    13081 ttaaaaggtg cggcaacggc ctgaataccc tcctggtgga gtttttctac agcaagttct
    13141 gcgcgttcgg cagtaatatc attaataatt atttgtgcgc catatttacc caggccggtt
    13201 gccagtaaaa agccaatgcc ctgtgctgaa ccggtaatca agatattttt tcctgccagt
    13261 gaaaatagat cgttcattgt attattcctt tatttatctc ttagaaaaca agctggactt
    13321 ttgctgcctg ggttttatca ccggcgaaac gtagcgcctc ttccaggtca gtgaagggat
    13381 attcagcact cagtaaaggc agtggattga taacgccatt cgccagccat gacactgcgg
    13441 tattaaattc gctggtaaaa cggaaagagc ctttgagtga aatctcctta ccaatcaatg
    13501 tcatcattgg gaattctgcc atcgcgcctc ccatacctac ctgcaccatt acgccgcgtg
    13561 cacgagtgac ctccagacag gtattcactg atgaaggatg accggacact tcaaagctga
    13621 catcgaaata gcctttttcc gctttccagt gatccatgtc gtcgttttgt gggtttacga
    13681 gcacatccgc ccccatctct ttacccagcg aaagggaacg gggactcaca tcggcacaga
    13741 caatttccgc ggcccccagt gttttcacag cactgacaat caggcagcca atgggcccaa
    13801 caccagaaat aaatactcgc ttgccctgta actcgccggc ctgatgtgcg gcatgaatcg
    13861 cgacggctaa aggttcggca aaagccataa ccttttcgtc agctttggcc ggataaggga
    13921 cacattgcga cgtttcgacc attttataac gggtaaaacc accatcaaca tgagggaaat
    13981 acatggcact gccaaaaaaa cgcatatctg tacactgatt ctcgttatgt tcaatgcagt
    14041 atttgcagtg accgcacggt ttagacggat taatggctac cgtttgccct tcatgtaatt
    14101 ctgatgagtc gctatgaata actttaccga taacttcatg acctaacacc atcggtgcct
    14161 ttatcatgaa attacctact tttccttcct gataataatg taaatcggaa ccgcaaattc
    14221 cacctcgggt tatttgtact aatgttccat tattattcca atctatcgtc tgctcggtaa
    14281 cggcaacagt tttcttgccc gcaacaacgc aggactgtgt tttcacttgc ataaattcct
    14341 cactggtcag gtagacacct cggaagcatt taagcggttt taattgttat tatttgtgat
    14401 gaagatcacg tcagaaaatt gttacattac tatgttacgc ataacgtgat gtgccttgta
    14461 attcttatca gcagaaataa aaaaacgtga aattattatg ccaccaggcg tagtatcgca
    14521 gcaggtaaga tgattcagga gattttaaat ggcgggtgaa agctttattt tgatgggcgt
    14581 ttcagggagt ggtaaaacat taattggtag caaggttgcc gcgttattat ctgctaaatt
    14641 tattgatggt gacgatcttc atccagccaa aaatatagat aaaatgtcgc agggtattcc
    14701 attatctgat gaagatcgac ttccctggct ggaacgctta aatgatgctt catacagtct
    14761 ttataaaaag aatgaaacag gatttattgt ctgttcatca ttaaaaaaac agtatcgtga
    14821 tattttacgc aagggtagcc cccatgttca tttcctctgg ttagatggcg actatgaaac
    14881 tattctcgcg cgaatgcagc gtcgggctgg gcattttatg ccggtagcgt tactaaaaag
    14941 tcagtttgag gcactggagc gtccacaaga agatgaacag gatattgtgc gcattgatat
    15001 caaccatgat attgccaatg tcactgaaca atgtcggcag gctgtgctgg cgatacgaca
    15061 aaacagaata tgtgcgaaag agggcagcgc ctcagatcag cgctgcgaat gattttcaaa
    15121 aatcggcttt caacaccacg cggtaacgcg ccttaccgtc gcgcacatgc tggatggcgt
    15181 cgttaatttt cgacatcggg aacaattcgg tagtcggcgc aaccttgctg cgggcggcaa
    15241 aacgcatcag cttacgcaac tcataaggcg tgccagtagc agagccagag acactgcgat
    15301 cgcccgcaat taacgtaaag gccggaacag acagcggcgt gagaaccgca ccgaccgtat
    15361 ggaaattacc gccatacgtc agcgcctcaa aataaggctg ccagtcgagg ctgacgttca
    15421 cagtattgat aatgagatca aactgccccg ccagagtttt cagtgcctgc ggatcgcggc
    15481 tattcaccac tttatcggca cccatcgcca gtacttcctg ctctttcgcc ggatttgaac
    15541 taaaggctgt cacctcgcat cccattgcgt gcagaagttt tatagcgata tgccccagcc
    15601 cgccaatacc aattacccca acgcggctgg tagcagtgat atggtgcatc aatagtggtt
    15661 taaagaccgt gataccgccg cacaacagcg gcccggcgga ctcgatatca atattttctg
    15721 gcagtggaat cacccattgc cagtccgcac gcaacttctc ggcaaagcca ccgcgattca
    15781 taatcgtcgg caccgcacct tgctcgcagt tgatctgatt accgctaata caggcgtcgc
    15841 agtgaccaca gctacgcgcc gtccagccaa tcccgacacg ctgaccgacc tgcaaacctt
    15901 tatcctgcgc ggcgctcccg agtgccacca cgcgcccaat cacctcatgc ccggcaacca
    15961 gcggatactg tgaaaatccc cattcgttat cgatcatcga cagatcggaa tggcagatcc
    16021 cgcagtaatc cacctgcact tcaatatctt gtggcttcag ctcaccggga tcgtactcat
    16081 aaacttccag ttcgccgccc gcttcttttg cggcatagct ttttatcatc gacatttttt
    16141 ggtccttctc tggtgttgtc tgggaagtgt agagcatggc agggcggggt gcctggagtg
    16201 tgacaaaggt tacacatcgc ggtatgcaat gctgaaaatt tcagcactta gcgaggtgcg
    16261 agcaagctag cgcttgcatg gtggtgcgtg acaggtataa ttcacaacgt tttccgcata
    16321 cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt gattcaaaat caaccgtaga
    16381 aatacgtgcc ggttcgagtc cggccttcgg caccaaaagc atgtaaatgg acctcaactg
    16441 aggtcttttt ttatgcctga aatccccgtc atacaaggcc ttccgcgatt ttcctttcaa
    16501 cagaagtcaa tctgagtcaa cccacatcaa gtcgcttgtg agtatagaac cgagtatata
    16561 tgctggttcg ataatggttg tatactcaca gcggcacatg gaaggagaac ttttatggct
    16621 ctgacagata ctaaagtacg ttctgcgaaa ccgcaggaaa aggagtacac cctagtcgat
    16681 ggggatggca tgtttttgct gattcatccc aacggctcaa aatactggcg gttccgcttt
    16741 cgcttcggtg gcaagcaaca cctgatggct ttcggtgttt accctgaaac ttcactggct
    16801 gatgcccgtc aaaaaagaga agaggctagg agacttgttg ctgctggaat cgaccctcgc
    16861 gagcataaac gtgctgtgaa agaagaacag gcgaaagagg ctattacctt ctgtcaacga
    16921 cggatgaaaa gtgatccact tatatctcca ccaacggccc aatattgatc caccgtttta
    16981 ctcaggatta gcttcagcta taaccccggc ctttcgtttc tgtctgagtc gatagctttc
    17041 tcctttgatt tgaacgacat gtgagtggtg taagatacgg tccagcatcg ctgaggtcag
    17101 tgctgcatca ccggcgaacg tttgatccca ctgcccgaac ggcagattgg atgtcaggat
    17161 cattgcgctc ttttcgtaac gtttagcgat gacctggaag aacagttttg cttcttcctg
    17221 actgaacggc agatagccta tttcatcaat gatgagcagg cggggggcca ttactccacg
    17281 ctgaagcgtc gttttataac ggccctgacg ttgtgccgta gataactgaa gtaacagatc
    17341 tgctgctgtt gtgaagcgaa ccttgatacc tgcacggact gcttcatagc ccatcgctat
    17401 tgccagatgg gttttcccca cacctgatgg ccccagtaat acgatatttt cattacgttc
    17461 tatgaagctg agtgagcgta acgactggag ttgcttctgc ggtgctccgg tggcgaatgt
    17521 gaagtcatac tcttcgaacg ttttcaccgc cgggaaggct gccattcggg tatacatcgc
    17581 ctgtttacgt tgatgacgtg ccagtttttc ttcatgaagc agatgctcca ggaagtccat
    17641 ataactccat tcctggtcta ctgcctgttg tgacagcgca ggcgctgcgc ttataaggct
    17701 ttccagttgc aactgcccgg cgagcgccat cagtcgttga tgttgcagtt ccatcatcac
    17761 gccactcctc tgcagaatga gtcgtagatg gagagtggat gatgcagggg gtgtttatcg
    17821 aagttcacca gattttcacc aggatgcacg tcatactctt ttttctccgg aggcagtgcc
    17881 agcatggact gctgctcttc gagccagcga tcgcagggac gggcctggat tgtttcatgc
    17941 tttcgttggt tagcgacatc gtgcagccag cgcagaccgt ggcggttggc tgtttcaaca
    18001 tcgacagtga tccccatcgg gcgcaggcga gtcattagtg ggatgtaaaa actgttacgg
    18061 gtgtactgca ccatccgttc caccttacct ttagtctgtg ccctgaaggg gcgacacagt
    18121 cggggagaga agcccatctc cttgccgaac tgccacagcg aaggatggaa ccggtgctga
    18181 ccggtctgat atgcgtcacg ttgcagaacc acagttttca tattgtcata caacacttcg
    18241 cgcggcacac caccaaagaa gcggaacgca ttacgatggc aggtctccag cgtgtcataa
    18301 cgcatattgt cagtgaattc gatgtacaac attcggctgt atccgagaac agcaacgaac
    18361 acgtgaagcg gtgagcgacc attacgcata gtgccccagt caacctgcat ctgtcgtccg
    18421 ggttcagttt cgaaccgaac ggcaggctcc tgctcctgag gaaccgagag agaacgaatg
    18481 aacgccctga gaatggtcat tccgccacga tatccctggt ctctgatctc gcgagcgatt
    18541 accgttgccg ggattttgta aggatgagca tcggcgatgc gttgacgaat ataatcccgg
    18601 tattcatcca ggagtgaagc aacagcaggt cgcggtgtat attttggcgg ctcagatttt
    18661 gcctgcaaat aacgtttaac ggtattgcgg gagatcccca gttctctggc aatcacccgg
    18721 ctactcattc cctgcttgtg caggatttta atttccataa ctgtctcaaa agtgaccata
    18781 agctctcctg aatcaggaga gcagattacc ccctggatct gatttcaggc gttgggtgtg
    18841 gatcactatt gcaccgttcg tgacaccttc gagtcagttg ccagagaatg gcacgctact
    18901 aataagaaat ggtccgaaga acacagccga agggtattga agagcttaga ggacaatttg
    18961 ttctcggcaa ttggcagacg aagtatcgaa gagctaaaaa ctcgtgacct gcttgcaccc
    19021 ataaaaatcg tggaagcgac aggccgcttt gaagttgctt ctcgccttca gcagagaacc
    19081 acagcaatca tgcgctatgc ggtacaaagt gggctgattg attacaaccc ggctcaagag
    19141 atggctggcg cagttgcttc cagtaaccgt gtccatcgcc ctgcacttga gctgaaacgt
    19201 ctacctgaac tgctccaccg aattgacggt tacacgggac gccctttgac ccgcctggct
    19261 gttgagctaa ccttattaat cttcattcgt tccagtgaat tgcgctttgc ccgctggtct
    19321 gagatcgatt tcgatacggc aatgtggacg ataccggcag aacgagaagc tatcgaagga
    19381 gtgaagcact ctcaacgtgg ttctaaaatg cgcacacctc atctggtgcc attgtctcat
    19441 caggctctgg caatcctcaa acaggttcac aagcttagtg gcgagcgcga ttttgttttc
    19501 attggcgatc atgatccgcg taaaccgatg agtgaaaaca cggtgaacaa ggcgctgcgc
    19561 gttatgggtt atgacactag ggtagaggtt tgtgggcatg gtttcaggac tatggcatgt
    19621 agctcgctga ttgagtcagg gctgtggtcg agggatgcgg tagagcgtca gatgagccat
    19681 atggaacgta actccgttcg agcggcttat atccataagg cagaacattt ggatgagaga
    19741 agattgatgt tgcagtggtg ggcggatttt ctagatgtaa atcgcgagaa agtgattagc
    19801 ccgtttgatt ttgccaaaat ttttgcgtcg ttaaagcgct aaagctgtgt aacaaaaata
    19861 gagcctaccg tcacactttg ttaagtaggc tcgttggtca aagttgcaac aatttcatga
    19921 tggtagctgt ccgcgcattg gtttcaggta gttctgcaga gtattgaacg tacctggaga
    19981 aaaaggcata tggtgctcaa ttgttagctt gtatagaata aggatattct ttctttagag
    20041 aacatgaagt tatgagcgcg atcacattac g
//
LOCUS       DAFAVF010000070        19819 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFAVF010000070 DAFAVF010000000
VERSION     DAFAVF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.070"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..352
                     /gene="ydiV"
                     /locus_tag="LGK42_002548"
     CDS             <1..352
                     /gene="ydiV"
                     /locus_tag="LGK42_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBK2658784.1"
                     /translation="NEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFTRVMLD
                     KSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHALQGCLW
                     PAVPINQITTLVQR"
     gene            415..1851
                     /locus_tag="LGK42_002549"
     CDS             415..1851
                     /locus_tag="LGK42_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBK2658785.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1855..2046)
                     /gene="hemP"
                     /locus_tag="LGK42_002550"
     CDS             complement(1855..2046)
                     /gene="hemP"
                     /locus_tag="LGK42_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBK2658786.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2178..3224)
                     /gene="aroH"
                     /locus_tag="LGK42_002551"
     CDS             complement(2178..3224)
                     /gene="aroH"
                     /locus_tag="LGK42_002551"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBK2658787.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAAHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3381..4214)
                     /gene="ppsR"
                     /locus_tag="LGK42_002552"
     CDS             complement(3381..4214)
                     /gene="ppsR"
                     /locus_tag="LGK42_002552"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBK2658788.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4547..6925
                     /gene="ppsA"
                     /locus_tag="LGK42_002553"
     CDS             4547..6925
                     /gene="ppsA"
                     /locus_tag="LGK42_002553"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBK2658789.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIELGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(6982..8628)
                     /gene="fadK"
                     /locus_tag="LGK42_002554"
     CDS             complement(6982..8628)
                     /gene="fadK"
                     /locus_tag="LGK42_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBK2658790.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(8685..8978)
                     /locus_tag="LGK42_002555"
     CDS             complement(8685..8978)
                     /locus_tag="LGK42_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBK2658791.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(8975..10264)
                     /locus_tag="LGK42_002556"
     CDS             complement(8975..10264)
                     /locus_tag="LGK42_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK2658792.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHTQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10320..11258)
                     /locus_tag="LGK42_002557"
     CDS             complement(10320..11258)
                     /locus_tag="LGK42_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBK2658793.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11278..12042)
                     /locus_tag="LGK42_002558"
     CDS             complement(11278..12042)
                     /locus_tag="LGK42_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBK2658794.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            12358..13269
                     /locus_tag="LGK42_002559"
     CDS             12358..13269
                     /locus_tag="LGK42_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK2658795.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(13312..14463)
                     /locus_tag="LGK42_002560"
     CDS             complement(13312..14463)
                     /locus_tag="LGK42_002560"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBK2658796.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(14477..16072)
                     /locus_tag="LGK42_002561"
     CDS             complement(14477..16072)
                     /locus_tag="LGK42_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBK2658797.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(16216..16974)
                     /gene="aroD"
                     /locus_tag="LGK42_002562"
     CDS             complement(16216..16974)
                     /gene="aroD"
                     /locus_tag="LGK42_002562"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK2658798.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(17005..17871)
                     /gene="ydiB"
                     /locus_tag="LGK42_002563"
     CDS             complement(17005..17871)
                     /gene="ydiB"
                     /locus_tag="LGK42_002563"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBK2658799.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(17883..19148)
                     /locus_tag="LGK42_002564"
     CDS             complement(17883..19148)
                     /locus_tag="LGK42_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658800.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSTLATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(19375..>19819)
                     /locus_tag="LGK42_002565"
     CDS             complement(19375..>19819)
                     /locus_tag="LGK42_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658801.1"
                     /translation="LLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHPTFYVV
                     IIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYSAGSIATFTIPLITAHLSQ
                     RSIADIMWFDTTIAAIGFLLALFIGLRSRKKTRHQSLKENVAPGG"
BASE COUNT         4952 a         4922 c         4912 g         5033 t
ORIGIN      
        1 caatgaagga aaagataatc gcgatctttt atccttatcg caaatgtatc cccttgtgtt
       61 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
      121 cgtcatgctg gataaaagtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
      181 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
      241 tgatacgaca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
      301 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
      361 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacaggagg atctatgacc
      421 ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc actttcccct
      481 acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt
      541 attccatcgt cgctgtttaa aaatgctgca ggtgtctggg gcggcgaaac cctactgcct
      601 ggtatgtcac cactggcgca ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa
      661 ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg cactacaatg
      721 gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg
      781 gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt
      841 ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg
      901 gagccaggcg cgatgctgat acgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa
      961 catttttact atcgccgcga gccggaaaag gttcgtcagt tagctgactt tgccattcgt
     1021 cattactggt cacatcttgc agatgatgag gacaaatacc gtctctggtt taccgatgtt
     1081 gtcgcacgta ccgcatcgtt aattgcccaa tggcagacgg tcggctttgc tcatggggtg
     1141 atgaataccg acaacatgtc gctgctgggg ctgacgcttg attacgggcc gtttggtttt
     1201 cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttatagc
     1261 tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca
     1321 ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg
     1381 acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca aaaagaggat
     1441 aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc
     1501 acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag
     1561 tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcggaggcg tttgcaacaa
     1621 gacgaggtta gcgatattga gcgtcagcaa ctgatgcaaa gcgttaaccc agctctggtg
     1681 ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgacggaa
     1741 ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc
     1801 agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt
     1861 agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc
     1921 gataatcagt ttgccatccg gacctaacag tgtctggctg gaaattcgcc gaataggctg
     1981 cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata
     2041 acgcataaaa attaataata attgatataa caatgattct tattatcaat attatggcta
     2101 accagagcaa gcagtaatgt gctgagaaga caacaagttg cgcagaaatg cccatcccat
     2161 caggaatggg cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg
     2221 cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc
     2281 tgactgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca
     2341 atcgccgtag agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga
     2401 cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca
     2461 aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg
     2521 ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga
     2581 ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg
     2641 gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgccgaa
     2701 gccatttcac ggtggatctg actttcggta gtacgtgcgc caatcgcgcc ccaactgatt
     2761 aaatcagcaa taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacaccc
     2821 agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag
     2881 ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt
     2941 tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc
     3001 agacgggtgg cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataata
     3061 accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg ggagtcggtg
     3121 acatgggcgg cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc
     3181 aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat
     3241 ttgttgcttc tacggcagga ctgccgggaa gtgatgcgca tcacgataaa ccaatcgctg
     3301 ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat aacaaaaaaa
     3361 taagctaatg cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc
     3421 ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta
     3481 cggtacaacg cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga
     3541 ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg
     3601 aacaatttgt gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg
     3661 gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg
     3721 ggggttttac cacagcgaga aacgccgagc aggatcacct gagcctggtc cagattgcgc
     3781 aacgaaatac cgtcatcgtg ggcaagggtg taatcaatcg ccgcaatgcg cgcatcatat
     3841 ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt
     3901 ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca
     3961 ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc
     4021 acacctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca
     4081 ttttcgacaa acggcagcgt aatgctgctg atagtgacgg gaaattgtga cattactgcg
     4141 tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca
     4201 acagcattat ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt
     4261 aaaaacgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcaattttat
     4321 tttttatgaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
     4381 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
     4441 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
     4501 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
     4561 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
     4621 gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg gtgtttccgt
     4681 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
     4741 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta cccagcttgc
     4801 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
     4861 aaatgccatc cgcgaagctt atgcacagct ttccgccgat gacgaaaacg cctcttttgc
     4921 ggtgcgctcc tccgctactg cagaagatat gccggacgct tcctttgccg gtcagcagga
     4981 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
     5041 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
     5101 cgtggcgctc tccgccggtg ttcaacggat ggtgcgctct gacctcgcat cttctggcgt
     5161 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
     5221 gggccttggt gaaatggtcg tgcagggcgc ggttaacccg gatgagtttt acgtccataa
     5281 accgacactt gcagcgaatc gcccggctat cgtgcgtcgc accatggggt cgaaaaaaat
     5341 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
     5401 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
     5461 ggccgtacaa attgagaaac actacggtcg cccgatggat attgagtggg cgaaagatgg
     5521 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
     5581 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
     5641 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
     5701 catcgaactt ggcgacgtgc tggtcactga catgaccgac ccggactggg aaccgatcat
     5761 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
     5821 cgctcgtgaa ctgggcattc cggcggtagt gggctgtggt gatgcaacag aacggatgaa
     5881 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
     5941 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
     6001 agtgatgatg aacgttggta acccggaccg tgctttcgac ttcgcctgcc tgccgaacga
     6061 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
     6121 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
     6181 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
     6241 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
     6301 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
     6361 cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcgctgga
     6421 gtgtgaagca gtgaaacgag tgcgcaacga catggggctg accaacgttg agatcatgat
     6481 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
     6541 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc
     6601 cttgctggca gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
     6661 gacacagctg gcgctcggtc tggatcgtga ctccggcgtg gtgtctgaat tgttcgatga
     6721 gcgcaacgat gcggtgaaag cattgctgtc gatggctatc cgtgccgcga agaaacaggg
     6781 caaatatgtc gggatttgcg gtcagggtcc gtccgaccat gaagactttg ccgcatggtt
     6841 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
     6901 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccgggttta
     6961 tgtgatcccc gaagataaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
     7021 cataatatct tttcttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt
     7081 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
     7141 agccaccacc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
     7201 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
     7261 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
     7321 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
     7381 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
     7441 atatcccata aacacattgg ggccacgcga ggcttcttca ccttcgcaac ctggcggtaa
     7501 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
     7561 gtgcataaag cgcgacaaag gatcatcgag attcaccacc gcatgcggcg aactttctgt
     7621 ggaaccataa acacttaata atttaatgcc gagctgctgg cattcacgcg ccactttttt
     7681 ggggattgtg gttccgccgc aaagaaagaa acgcagcgct gacaggtccg cgggttgttt
     7741 ctctagtaca ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
     7801 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
     7861 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt ggccgagtgg
     7921 cgcaggcatc ataaagacat cctgccaggt cagattcagc cgcgcgcaat aagcccgctc
     7981 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
     8041 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtggggt
     8101 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
     8161 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
     8221 gcgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
     8281 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
     8341 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
     8401 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
     8461 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
     8521 cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca tcgccccata gcccttgctg
     8581 acgatacgcc gcacgacgtt gttcgttgaa cgttaatgtc actttcatgt tggactctcg
     8641 aaacagaaca tcaggcccga gatgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
     8701 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
     8761 acccgacagg tgccgcactc cagacaaccg gcagaatcaa aatgaatgtt gccggcgtca
     8821 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
     8881 ggatttgccg ccagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
     8941 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
     9001 catctttcag caagttgatc agcccaattt tcttcgcatg tcccatgatc attttgcgta
     9061 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gcgaccattc
     9121 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cggaaatgct
     9181 gcatatcgcg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgctgg
     9241 cggagaaatc ctcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
     9301 caatggctaa atccatgcca cgaactgtaa aacccaaatt caggcagaag cctgcggcgt
     9361 caccaacgat catcacgcca tcgttaacca tctgcggcac cattgccaga ccgccttctg
     9421 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aattagtggg cgaatggcgg
     9481 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgtatgg gcgatatcac
     9541 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
     9601 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
     9661 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
     9721 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
     9781 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
     9841 tgaccttgtt tccttcacga accagcgcat cgacgcgaac gcctgggata aactgcgcgc
     9901 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
     9961 atgcgtgttg cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    10021 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    10081 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    10141 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgc gccatgacca
    10201 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcttccg
    10261 acatgcgtta tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat
    10321 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    10381 tagtcggcat aattgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    10441 gcgccgttgc cgccaaccat atgctggatc tgcccggaga tccccagcgt caggtagaga
    10501 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    10561 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    10621 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    10681 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    10741 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    10801 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    10861 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gataatatcc
    10921 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    10981 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    11041 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    11101 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    11161 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    11221 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    11281 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    11341 gataatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    11401 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    11461 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    11521 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    11581 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    11641 aatttctccg accagcaagc cagcctgctg ggcataaagg tcgccggaac cttcaccaaa
    11701 gatcagtaaa tcgaagccga tcttttcaac tgctgccgcc agcgcttttg cggtatcgag
    11761 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    11821 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    11881 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    11941 gctgattttg gcgtctgcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    12001 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    12061 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    12121 gcggggtttt cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga
    12181 tcgctaaaac aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc
    12241 attcagtgta aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac
    12301 gccgtatttt atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg
    12361 tatcaacgct gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg
    12421 ctttcacgtt ttgcaattag cgatgatcca aaatgggagt ctggacatca cgttcatgac
    12481 aatgaaaccg agctgattta cgtcaagaaa ggggtagcaa ggtttaccat cgattcatcg
    12541 ttatatgtcg cacatgccga tgatatagtg gtgatagaac gcggcaggct gcatgcggtg
    12601 gcctctgacg ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt
    12661 cagggggctg aggaaaatca gctactgcaa ccgcattctt gtccggtaat tgccgcgggg
    12721 cagggaaaag aagtcataaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt
    12781 aaaaatagcc aaacatcttc gttatgggat gcatttgcct atacgttagc cattctttac
    12841 tacgaaaatt ttaaaaatgc ttatcgttcg gagcaggggt atattaaaaa agatgttctg
    12901 ataaaagata ttcttttcta tctgaataat aattatcgcg aaaaaatcac tttagaacag
    12961 ttatcgaaaa aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat
    13021 cgtatttccc ctattaacta tgttattcaa cggcgtatga cggaagcaaa atggtcactc
    13081 actaatactg aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat
    13141 cactttgcca aactgttttt gcgccatgtc ggttgttcgc ccagcgatta ccgtagacaa
    13201 tttaaaaact gttttgcgga acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt
    13261 gttggataaa tctggcggcg cagtttgtta agaagctgcg ccgcctgcag attatttgtt
    13321 ctgatagtct ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc
    13381 gccgatacgt tcacaacgga catcacgcca gaagcgggag acgcgcgctt catcggtata
    13441 gcccagaccg cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcgca
    13501 atacagtttt gccagcgccg cgctggtgcg cagtgactga tgctgatcgg cttgccatgc
    13561 cactttcagc accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc
    13621 ctggatcatc tggttatgac caatgggctt accaaaagcg atacgttggt tggcatagcg
    13681 ggcggcatct tcaaaggcgc attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg
    13741 ctccatctca aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc
    13801 gctctcttca acctccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc
    13861 gattttatgc agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt
    13921 gaaggctttt ttgggatctt tcggttgcgg atcgcgcgcc aacaccagca tatacgggta
    13981 ctctttcgca ccggtaataa aggttttttg tccgttgatg tagaccttgc cgtttttacg
    14041 cgtgtaagtg gtagtggcac tgttgttatc tgagccagca cctggttccg tcaacgccag
    14101 ggcataggcg ggatcacccg tttccagggt gctttctgcc gttttacgta gctgctctgc
    14161 agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg gtgatcaaaa atgccggagc
    14221 accgcatttt gacacttcca tcagcgccag catttgggtg acgtaatccg cagggatacc
    14281 accaaattct tccggcacgc caagcatgga aataccgtta tccgccagcg cccgcataaa
    14341 ctcacgcgga tatgtcccgt tttgatcgca ggtgcggaaa tactcttccg gaaagttagt
    14401 cgtaatcagt tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc
    14461 catcattata ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga
    14521 taaataatct ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aagtccattt
    14581 tgtcgagaat atctttttgt aaatcgacgc caggggcgat ttcgattaaa tgcaggccgt
    14641 cttctttcag cgtgaataca gcacgttcgg tgatgtaacg aacatccagt ccccgttcga
    14701 gcgcgatttt tccgctaaaa gtaatttccg gtagttcccg aataaattta tttacccgtc
    14761 cttcctggac gatatgcaat ttgccatcgg caatttctgt ttttaaactg cccgcagtta
    14821 acgtgccgca gaaaataatt ttcttcgagg tggcactgat atcaataaat ccaccggtac
    14881 ccatgatttt gccattgaat ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa
    14941 aactcaaata acaaacatcc agaccaccac cgtgataaaa atcaaactgg gacgtcatat
    15001 ccagaatggc gcgggtattc acgttcgcgc caaaggcgat accctgtgaa gtaattccgc
    15061 caatcggacc tgtttctacc gtcagaataa agtcatcagc acaaccttct tcccgggcga
    15121 ccaggccaat accgtcagca ataccgacgc cgacattccc caccgcgcct ttacgcattt
    15181 cgaataatgc acgccgcgca actaatttac gttggtttag gggcaggcta agtttggtgc
    15241 tgtcatcaag ggtgaagtca ccagaaataa aacggttaac cggcgcaccg ccatacagtt
    15301 ggctttgatc cggatcgacc accacaatat ccaccagata acccggaata cggacagatt
    15361 taggatgcag cgtggctttc ttaaccattt tctgcacctg catcatcaca ataccgccgt
    15421 tattgtgcac cgcctgggca ataaccaatg cgtcgagata catcacctca tcttcaaaag
    15481 tggcgtagcc ttcactgtcg caggtggtag cgcgaatgaa ggcgatatct ggcgcaatcg
    15541 ctttgtaata gagatattct ttgttatcaa actcgaccag tttaatcagg tcttctttcg
    15601 tgacttcatt cagtttgccg ccttgctggc gtggatcgac aaatgtcccg atgccaatat
    15661 cactaataat accaggctgg tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt
    15721 gtggatagtt ataagcaata attttatttt gttctgcgag ttcagaaata cgcggcgatt
    15781 gtccccagtg accacataat gcccatttca ccagaccttc ttgcgccaga ggactaatac
    15841 cgcggtcggc gcgatcgcca agccctgttg gactaataat cgataaatta cgtggcgttt
    15901 gagtctgttt atatttatca gcaagagcag taattaacgt ggtggcttcc agaataccgc
    15961 cgccagcgcc taacacacaa agtgttgctt cgtcgggaat ataattcacc gcttcctgtg
    16021 ccgacaggac cggcactcgt ccattaatac gaggtggttt tacaggtttc attgttaatt
    16081 cctgattcag atgtgatttt ccctacgcca gatggagaat tagcgcacag agactcacga
    16141 aatattttta ttaaatacgg ttaaatatat tttttaagtt cggcggggag ggtgttcccg
    16201 ccgaaatatt attgcttatg cctggtgtaa aatagttaat accgtgcgta aatcatttac
    16261 cgagatttgc cctggtgcag aggctttttt taccgcacca aaagttgccg ccgagccaaa
    16321 tacttcacca gccagacgag aaattacgcc agtttttgcc atcgacatcg tgatgattgg
    16381 acgatcggca tactgctcct gcatctccag ggtcgcggta agcaacgtca gcacatcgct
    16441 ggtactttgc ggcatcagcg caatcttagg aatatcggcg tcgaaggact gcattttgcg
    16501 cagacgggca atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac
    16561 aactttcaca tcatgcgcgt gggcgtaggc gacggtttct ttaacctgat catcgccggt
    16621 aaataactcc agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagagc
    16681 aatataagcc tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa
    16741 cagcagcggt ttttctggca tggtttcacg gagaattttt gccgccgcca tgacagactc
    16801 cacattggag aggtcggcaa agtggtccac acgccattcc agaatatcaa agtccgcttc
    16861 acgataggcg agagcttcgg atttcacgcg ggcgatatct ttcgccatca gcgagacgat
    16921 gattttaggt gcgcccgtac caatgacgag atcttttaca gttacggttt tcatttttta
    16981 ccctttctgc acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc
    17041 cagagggaaa tctttgccag tccataacgt gaactgttca gccccttgcc acaacaacat
    17101 gccgtatcca tcaatcgttt tgcaaccagc ttgttgcgcc tgctgcaata acttcgtcat
    17161 atgcgggtta tacacgcatt cagtgaccag cagtcccgga tgtaacagac tgatatcatt
    17221 aactaatgat tcattctcaa ggggtttcat acccactttt gtgccattgg ttaaaatgtc
    17281 ggcggaagcc agggcttcag caaaggcttg ctgatcggcg agatcggtaa ccgtgacgac
    17341 acaatcggtg ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc
    17401 ccgacggtta aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaatcgc
    17461 cgttgaggca cctccggccc ctaacagcac cattgttttg cctttgatat caaaaccgct
    17521 ctctttaatg gctcgaatat ggcccgtgcc gtcggtgtta tagccgcgca ggtaaccatc
    17581 atcattaacg atggtgttga tggcacccac cagtttggct gctggcgtta attcatcaac
    17641 atattcacac gccagttgtt tgttcggcat tgatatacca gttccgcgca ttttgagggc
    17701 ttttaatcct tcaattgctc cgggaaaact atcgttatcc acttcaaagg ccatataggt
    17761 aaatggcaat cccgcttttt ctaaggcttt attctgcatt tcaggcgata aactgtggcg
    17821 gataggatag gccatcagcc caatcaattc gtattttgcg gtaacatcca taattaactc
    17881 ctttaacctc tatgcttaat tgtgtttaat cgggtgctaa atttgcgctc gccaaaccgc
    17941 acatcattct caggaataat aaaaacgcgg taatagcgga taaaaacaat aattgcggta
    18001 atcagggcca gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc
    18061 gacagataac cggtaatcag cggaataaca aagttagcca gtccacccat catcatataa
    18121 atactggtga ctttggcttt gctttttgga aaaaactctg acatgaccga aacaccgagc
    18181 tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg cgccggcatt acacaccagt
    18241 ggagaagggt acaggtagat aatggctgct gttattgttg ccagtgtaga gttaaataca
    18301 ttagcccaga tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagacc
    18361 aacgagccca tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca
    18421 aaagccatcg catatttggg catccacacc acaatcacat aaaaggtcga gaatgcggct
    18481 acaccgaaca gtaccgatga aacaccttcc agccagacta acggtttgct gttcatttgc
    18541 ggtaattcat tagctacgct ggcgtccacc aactggctgg ggaatttgct tttcaacagc
    18601 atcagcgtga tcagtacaaa taggatgcct ggaataatca gcccatagcc gtaccagatg
    18661 ttattgagca acatatagct caccagcatt gggtagaaca tttgtccgaa tgacaccatc
    18721 gctttaacca gtatgaccgc cgaaccagag gctttcggaa agcattccat gagcgcgggg
    18781 tagccacccg tatccagcgc tgagttagcg atacctacgc acactgccag accgtaggcg
    18841 agagttaaat tcgggcaagc gggaatacca aagaagaata gcagatacat tattactgcc
    18901 attaatatca ccgcccgacg accaaactta tcggagatca caccgaagaa taaaatactg
    18961 atcaatcgcc ccaaaccgat accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa
    19021 aacttttccg ccagagatga catgttttgg gcaagcgtaa taacactaat gccgtgcagg
    19081 aagtagctga agtaaataca aagaacagcc aggataaatg gcgtgctgaa agccttattt
    19141 tgagacattt tcacagctcc ttgctgaaag ataatttcgt taccgtacgt attgttctcc
    19201 gacggtttac ataattacta tactgaagat gattttccct gggtacatgg cataaagttt
    19261 atgtttccag gaatattgca tgtcatagtt tgtttttgat cgcgattttc cttagttagc
    19321 gcagaaagag ttatttatcc gtaagatctt gcatgacctg aaaagaatat tgcattaccc
    19381 acccggagcg acattttcct ttagcgactg atgccgcgtt tttttgcggc tgcgtaagcc
    19441 gataaacagt gccagtaaaa aaccgatggc agcgatggtg gtatcgaacc acataatatc
    19501 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
    19561 gcccgcactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
    19621 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
    19681 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
    19741 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
    19801 aataaataca caaagcagc
//
LOCUS       DAFAVF010000071        19816 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFAVF010000071 DAFAVF010000000
VERSION     DAFAVF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.071"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            227..727
                     /gene="tssB"
                     /locus_tag="LGK42_002566"
     CDS             227..727
                     /gene="tssB"
                     /locus_tag="LGK42_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBK2658802.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            762..986
                     /locus_tag="LGK42_002567"
     CDS             762..986
                     /locus_tag="LGK42_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658803.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITA
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1037..2512
                     /gene="tssC"
                     /locus_tag="LGK42_002568"
     CDS             1037..2512
                     /gene="tssC"
                     /locus_tag="LGK42_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBK2658804.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2519..2932
                     /gene="tssE"
                     /locus_tag="LGK42_002569"
     CDS             2519..2932
                     /gene="tssE"
                     /locus_tag="LGK42_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBK2658805.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2936..4786
                     /gene="tssF"
                     /locus_tag="LGK42_002570"
     CDS             2936..4786
                     /gene="tssF"
                     /locus_tag="LGK42_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBK2658806.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4750..5832
                     /gene="tssG"
                     /locus_tag="LGK42_002571"
     CDS             4750..5832
                     /gene="tssG"
                     /locus_tag="LGK42_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBK2658807.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5857..7137
                     /gene="tagH"
                     /locus_tag="LGK42_002572"
     CDS             5857..7137
                     /gene="tagH"
                     /locus_tag="LGK42_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBK2658808.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQHSIPAQAFTIILHDGTFCLRPQTVQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MMRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINSPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPKNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7134..7658
                     /gene="tssJ"
                     /locus_tag="LGK42_002573"
     CDS             7134..7658
                     /gene="tssJ"
                     /locus_tag="LGK42_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBK2658809.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7661..8992
                     /gene="tssK"
                     /locus_tag="LGK42_002574"
     CDS             7661..8992
                     /gene="tssK"
                     /locus_tag="LGK42_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBK2658810.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TELSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8997..9758
                     /gene="icmH"
                     /locus_tag="LGK42_002575"
     CDS             8997..9758
                     /gene="icmH"
                     /locus_tag="LGK42_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBK2658811.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQEQGEFEQIYRRLYHVLHKLRGNAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            9767..12526
                     /gene="tssH"
                     /locus_tag="LGK42_002576"
     CDS             9767..12526
                     /gene="tssH"
                     /locus_tag="LGK42_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBK2658812.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAAQLLTGINRDRLQQDFVQWTQESAESV
                     VPDADCKGAGALTDVADSLLARYAKNMTEDARNDRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQTLVQEIIALRQQLLGMAEDD
                     AASLPHVDAVEDTPPESEQDNTGAEPADEAGSEQPEETAETVSPVQRLAHLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAEN
                     GARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTK
                     DETVL"
     gene            12523..13266
                     /gene="tagO"
                     /locus_tag="LGK42_002577"
     CDS             12523..13266
                     /gene="tagO"
                     /locus_tag="LGK42_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBK2658813.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13271..14683
                     /gene="tssA"
                     /locus_tag="LGK42_002578"
     CDS             13271..14683
                     /gene="tssA"
                     /locus_tag="LGK42_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBK2658814.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14792..18226
                     /gene="tssM"
                     /locus_tag="LGK42_002579"
     CDS             14792..18226
                     /gene="tssM"
                     /locus_tag="LGK42_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBK2658815.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKALNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18237..19589
                     /locus_tag="LGK42_002580"
     CDS             18237..19589
                     /locus_tag="LGK42_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBK2658816.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            19613..>19816
                     /gene="hcp"
                     /locus_tag="LGK42_002581"
     CDS             19613..>19816
                     /gene="hcp"
                     /locus_tag="LGK42_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBK2658817.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKS"
BASE COUNT         4615 a         5478 c         5518 g         4205 t
ORIGIN      
        1 gacgcgaatt gaacaggttc atacaatcta tttaacgctt gcagaatacc cctctttacg
       61 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
      121 tggtgatttt attttcaccg ccatcaaggc ggcatttccg atattttttg cggtacgaat
      181 atgccgtgtt catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt
      241 tgaaggcagc gtcgcgcctc gcgagcgtat taatatatcg tatgtcccaa aaacagatgg
      301 ccagacggct gaagtggaac ttccgcttaa catgctcgtt gtcggcgata ccggaaatac
      361 gcaggaaaca tcctcactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg
      421 tgcagtgatg gctgaagcag ccattggcct caattttacc gtccccgcca cgctgaaggg
      481 aagcacaacg gacgatgagc tgaatgtggc gctgaatatc aagtcactgg atgatttttc
      541 accagatagc gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc
      601 actgactgca ctgaaggggc cgatgggtaa ccttccggca ttccgtaccc agctccaggc
      661 attgctggaa aatgaagagt cccgcgagca gttgctcaaa gaaatcggcc aggtgagcaa
      721 taaataacca ccactatttc attaaacagg gttatccagg catgacggca cacaaaaata
      781 tctccgggac ataccacctc tctgttgcag acattctgca ggtggtttac caggtatgtt
      841 tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc gctgattaca gcactggatc
      901 gccgtatcag tgatctattg gatgagatta tccatttttg tgagtttcag cagagcgcgt
      961 cgcactggca gcgcgtactt cactgacggc gaaaagccgg tttcagaaac acagctaaaa
     1021 cagaaggatt ttcagtatgt ctttacagga agaggaactc gtatccagtc atgccgggca
     1081 gcctgagcag gcatcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcccgg
     1141 ttcggaaggt tatgacgttg cccgccaggg tgtgacagcc tttattgcca gcatcctgca
     1201 atccactgca tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat
     1261 tgatgaacgc atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt
     1321 cgagtcgttc tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat
     1381 caaggtcaac gtgcttcatg tcaccaagca ggaacttctg gaagactttg agtttgcgcc
     1441 agaaattatt cagtccggat tttataagca cgtttattcg tccggtttcg gacagtttgg
     1501 cggtgaaccc atcgctgccg tgctgggcgc ttatgagttc aaaaataccg cgcctgacat
     1561 gaaactgctg caatacgtca gtgccgtggg cgcaatggcg cacgcaccgt tcctgtcctc
     1621 cgtttccccg gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct
     1681 ttatgccatc tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga
     1741 ttcgcgctat ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac
     1801 agataaccct gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta
     1861 tctgtggggt aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata
     1921 ccgctggtgt ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccggt
     1981 gcatctgttc gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac
     2041 cgatcgccgc gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga
     2101 ctctgataat gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg
     2161 aaaagatgca gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa
     2221 tcggttagcg cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag
     2281 gagcgactta gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa
     2341 cccgcctgca gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga
     2401 tgtagaaggc gaacccggct ggtaccaggt tgcgctaagc gtgagacctc attttaaatt
     2461 tatgggggca aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat
     2521 gataccggat cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcggc
     2581 cagaaaccgg cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctacg
     2641 aaacattctt aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga
     2701 accggaaggg gctgaaaact tccgcgaatc catgagtcgg gccatcgagc aatgtataga
     2761 acgctatgaa ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag
     2821 cccgctggat atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt
     2881 gcttgaattt gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga
     2941 atttgaagaa cgctatttcc gggaagaact cgattacctg cgccagctta gcaagctgct
     3001 ggcaacggaa aaaccccatc tggcccgctt cctggccgaa aaagatgcgg atccggatat
     3061 tgaacgcctg ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga
     3121 tgaattccca gaactgacgc acgggcttat taagatgcta tggcctaatt acctgcgtcc
     3181 ggttccggca atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt
     3241 gttaatcccc cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt
     3301 gctgccctct gatgctaaaa aggaggagcc gcctccctgt accttcaccc tctgccggga
     3361 tatctggctg ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg
     3421 tgttatcaac atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa
     3481 caaacttcgc ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg
     3541 gttctgcgaa tacttgcagg gtgccgacct gactgtgggt gaacagcata ttcgcctgcc
     3601 tgagtttatg ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa
     3661 aaacgtccac agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct
     3721 cttttttgat ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac
     3781 cctgcaactg cgtttttcgc gccctttgcc cgtggacatc cggctgcgcc gcgattccct
     3841 gcgcctgtat tgcgcacctg ccattaattt atttatccac catgcagaag ccatcacgct
     3901 ggacaaccgg cgggcagact atccgctggt tcccagccgc cattacccac aacattacga
     3961 tgtattttcc gttaacagtg tggtgagcca ggtccaggat atgttcagga aaaaagatct
     4021 ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca gcctttgaaa gtttcagcca
     4081 tcagatggaa tacagccgga agcgggaagt ggtgtactgg catcaccgga ccaaaacatc
     4141 cctgttccat cgcggctttg atcataccct tgcctttata catgctgatg gcagttatcc
     4201 gtcagacgaa tctctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg
     4261 tgagcttccg tcacaaattc gttccggcga tatcaccggc acaaccggta aaaatgcggc
     4321 tgttgcttca tttcgcaaca ttacccgccc gacgcaacca ctctggccgg tcattgatgg
     4381 cagcctgcac tggtccctac tctccgccat gaacctgaat tatctgtcat tactggatac
     4441 ggacgcgctg aagcaggtca tcgccaactt tgatcgccac gcaatccatc atccgcagac
     4501 ggcacggctg tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccccgttga
     4561 tcgcctgttt acgggtattc ccgtccgggg actggcctcc acgctgtatc tgcacccgga
     4621 gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc
     4681 gctgtacgcc agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga
     4741 gacatggaaa tggacggaaa gaatcgggca gcatcctctt atctgagtcc gggaaatccc
     4801 ccggcagata aagagcagaa tgatccgctg gcacaggttt tccataatgc ctgctcttac
     4861 aatttttttg cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca
     4921 gaattatccc tgcgtgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc
     4981 cttgccttcc cgtgcagtga tatcagtgcg ctgaaacggg atacgtcagg ggcattcagg
     5041 atgaccacca cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc
     5101 gatcacctgg cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg
     5161 ttcagccacc gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc
     5221 agttttcgta acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg
     5281 ggccaccgcc agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc
     5341 gggatcctgg caaacccggg gcgatccccg gaaattatct gtggcctggt gtcccactgc
     5401 tttgatttgt cagaagtgac gctgcaaaac tggcagcgtc gcaaggtgga tattgaaccg
     5461 gatcagcaaa atagtctggg gagctattca cttaaaaatg gcgaaaaact ggcgggaaga
     5521 tcggtactgg gcaactttgt tctgggtaca cgggtgcccg atctcagcgg aaaattccag
     5581 ctcagtatta ccagcctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac
     5641 ttcctgccac tgacgatgtt tgtgtccttc atcctgcgcg atcagcttgc ctgggactta
     5701 catctggggc tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg
     5761 ctgctgggct ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg
     5821 gtacggtcgt aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg
     5881 ctttcattgc aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtctg
     5941 ttcacacagc agggaaatgt cggccatgcc cccgaatgcc actggtcggt acaggatcgt
     6001 cagcatagca ttccggccca ggcttttacc attatcctgc acgatggcac attttgtctg
     6061 cgcccgcaga cggtacaact gtggctgaac caggcaaaag tcacagcaac atcagacctg
     6121 atacagctgc gccagggcga tgagatccag atcgggcgac tgatgatgag ggttcatctg
     6181 aaccggggag atatccccca ttacgatgag gaaatggcga cgcccgaaac catcgtcacc
     6241 aatcgcgata tgctcacgga taccctgctg tcaacggagg gtgcgccaca ttatccggga
     6301 atgactcacc ggcaccagct tgcagacacc gtggtaaacg gtttttctgc cgatccactc
     6361 caggcacttc agtccgaaag cctgattacc acaggcgatc cgctttcagg cattgcggct
     6421 gtccggccct cggcaccgct gtccgatccc gcaagtaatg gcgggatcaa ttcgccgttt
     6481 atggatctgc cgcccattta tgccagcccc ggcgatcgta atgatgacgt ctctgcggca
     6541 gaaatggcgc aacgccacct tgcggtcacc cccttactgc gcggtctcgg cggctcgctt
     6601 accgtgagca attccgacga tgcggatgat tttctggagg aggccggacg aaccttacag
     6661 gccgcaataa aaggtctgct cgatttgcag cagcagcgta acagcctctc agacaaacat
     6721 ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac
     6781 gtgatgtttg ccgaaggcaa aagcccggta catctggcgg cccccgccgc cgtcagtgaa
     6841 agcctgcgca atgtccgcca ccacgaagaa gctaaccggg cggcgattgt ggagtcactt
     6901 cgtgtcctgc tggacgcctt ctcacctaaa aatctgctgc gccgctttgt gcagtaccgc
     6961 cgtagccatg aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat
     7021 tattacgaag aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc
     7081 tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca
     7141 ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga
     7201 aaaaaatcag tgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggagcaac
     7261 cttcggaaat aaccgtcacc ctgctgaccg aaccggacac caataccaat gcggaaggtg
     7321 aatcagccgc cgtggatgtg caactggtgt acctgactga cgactcaaaa ttgctggcag
     7381 ccgactatga ccagatagcc agtacgccgc taccggatgt gctgggtaaa aactatatcg
     7441 accaccagga tttcaacctg ttgccggaca ccatcaaaac cctgccgccg gtaaagctgg
     7501 atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc cgatgaccag gccactgaat
     7561 ggaaacaaat cgagacggtg gaaggaaccg gccaccatta ccgcctgctg gtgcatgtac
     7621 gtcaaagcag catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa
     7681 ggtgatgtgg caggaaggga tgctgatgcg cccacaccat ttccagcaac agcagcgtta
     7741 caacgactac ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac
     7801 cgaactcacc ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc
     7861 aggcacactg cccgacggca ccgtcttttc tatccccgac caggacgcac tgcccgatcc
     7921 gctgcacccg caacattttc cggacgagag aagccgcaat atctacctcg ctctgccggt
     7981 cgccagtgat gtgagaaatg aaatcagcga cgggcggcga atcgggcgtt accggctgaa
     8041 ttatgccgat gtccgggatt tgcattcaga agaaggtgac gcgcgaacac tgacgctggg
     8101 acaactgacg ccgcgcatta tgagcggtgc agaagatatg agcgcctata ttacgctccc
     8161 gctttgccgt atcagcgatc gccatgccga cggctccctg acgctggatg acgattttat
     8221 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     8281 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     8341 cggcatcgcg gatgtggcgg aatttatgat gttgcagttg cttaaccgta accagacccg
     8401 gtttacccat cgcgcccgcc gatcccagct ccacccggaa gatttctatc ttgatcttgc
     8461 cgggttgctg ggtgaactga tgacctttac agagctgtcg cgcctgccct gcccgcttga
     8521 tgtgtatgat catcatgacc tgaccaaaac atttaaaaca ctgttaccgg aagtcaaacg
     8581 agcgctgcat accgtactgt cccccagagc cgtcaatctg ccgctgcatc tgcgtgacgg
     8641 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
     8701 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
     8761 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atcccgggta ttcctctgcg
     8821 cgcactgatg gtggcccccc gccagcttcc ctaccattcc gggttcagct atttcgaact
     8881 cgacaagagc ggccaggcct ggacagaaat ggctgccgcc ggagccgttg cactgcatgt
     8941 ctccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
     9001 atgaggggtc actgtctctg ccaccattta ccggatacga cgaaaaaagc ctgcgcgact
     9061 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctcc
     9121 tcggtatggt gatgcgcctt tccactatga acagccagac gatgccggag catctttttg
     9181 ctcaggtggt gacggatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
     9241 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
     9301 gtaacggctg gtcgaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
     9361 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
     9421 aacgttacca ggatctgctg gaatttctct ggctctgctt ttctctggga tttcgcgggc
     9481 gctacaaagt ggcagcacag gagcaggggg aatttgagca gatctaccgc cgcctgtacc
     9541 acgttctgca taaactgcgc ggcaatgccc ctttcccgct gttacaccag gataaaaaaa
     9601 cccagggcgg gcgttatcag ctcatcagtc gcctgaccat taaacatatt ttttgtggtg
     9661 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
     9721 aggacatcct gtaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
     9781 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
     9841 ctctgaatgt atgagccagc aggcagcgga aattaccgtc agccatgtgc ttattcagat
     9901 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggtgata ttggcatgga
     9961 agaactgcgc caggcgctga cggtggagaa ctacacaacc gcccgttctg cggacagcta
    10021 cccggcgttt tccccgatgc tggttgagtg gctgaaagag ggctggctgc tggcgtcggc
    10081 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcacc
    10141 gctgcgttac ataccgcctg ctgccgccca gctgttgacc ggcattaacc gtgaccgtct
    10201 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    10261 ctgtaaaggc gcaggcgcac tgacggacgt cgctgacagc ctgcttgccc gctacgccaa
    10321 aaacatgacc gaagatgccc gtaacgacag gctcgacccg gtactgtgcc gcgaccacga
    10381 aatcgacctg atgatcgaca ttctctgccg tcgccgtaaa aataacccgg tggtggtggg
    10441 cgaagcgggt gtgggtaaaa gcgccctgat tgaagggctg gcgctgcgta tcgtggcagg
    10501 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacctgg gcgcattgca
    10561 ggccggggca tcggtgaaag gtgagtttga gaaacgcttc aaagggctga tggcggaggt
    10621 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcgcataccc ttattggcgc
    10681 gggcaaccag cagggtgggc tggatatctc caacctgctc aagccggcgc tggcgcgcgg
    10741 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    10801 tgccgccctg tcgcgccgat tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    10861 cactattatt ctgcgtggtc tgtcggcggt ctatgaacag tctcacggtg tgctgattga
    10921 cgatgacgcc ttgcaggccg cagcaacact gagcgagcgt tatctctccg ggcgtcagtt
    10981 gccggacaaa gccattgatg tgctggatac tgcctgcgcc cgcgtggcaa tcaacctttc
    11041 atcgccacca aagcaaatct cggcgctgac caccctgagt caccagcagg aggcggaaat
    11101 tcgccagctt gagcgcgagc ttcgcatcgg gctgcgtacc aacacctcgc gaatgacgga
    11161 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggagg ccgcctggca
    11221 tcagcaacag acgctggtgc aggagattat cgccctgcgc cagcagttac tgggtatggc
    11281 agaagacgat gcggcgtcgt tgccgcacgt ggatgccgtg gaggatacgc cgccagagtc
    11341 agaacaggat aataccggtg ccgaaccggc tgatgaagct ggcagcgaac agccggaaga
    11401 gaccgctgaa acagtttccc cggtacaacg tctggcacat ctcactgccg aactggacgc
    11461 cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga ttgcggcggt
    11521 gattgccgaa tggaccggcg tgccgcttaa ccgcctgtcg cagaatgaaa tgtcggtcat
    11581 caccgacctg ccaaaatggc tcggcgacac catcaaaggc caggacctgg cgattgccag
    11641 cctgcacaaa cacctgctga ccgcacgcgc cgacctgcgc cgtccgggac gcccgctggg
    11701 cgcgttcctg ctggctggcc ccagcggcgt gggtaaaact gaaaccgtcc tgcaactggc
    11761 agagctgctc tacggcggtc gccagtacct gaccaccatc aatatgtccg agttccagga
    11821 gaaacatacc gtctcgcgac tgattggctc ccctccgggc tacgttggct acggtgaagg
    11881 cggcgtactg accgaagcga ttcgccagaa accctactcg gtagtactgc tcgatgaagt
    11941 ggaaaaagcg cacccggatg tgctcaacct gttctaccag gcgttcgata agggcgaaat
    12001 ggcagacggt gaaggccgcc tgattgactg caaaaatatc gtcttcttcc tgacgtccaa
    12061 cctcggctac caggtaatag tcgagcatgc cgatgacccg gaaaccatgc aggaagtact
    12121 gtatccggtg ctggccgact tcttcaaacc tgccctgctg gcgcgtatgg aagtggtgcc
    12181 gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac tggcccgcct
    12241 ggataacgtg ctgcgcagcc gctttggcgc ggaggtgatt atagaaccgg aagtgacgga
    12301 cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg aggatgctgg aatcggtcat
    12361 cgacggcaat atgctgccgc cgctctccct gctgctgttg cagaaaatgg cggcgaatac
    12421 ggcggttgcc cggattcggt tgtcggcagt ggacggcgca tttacggcag acgtggaaga
    12481 tgctcagaac gacgagtccg tcacaaagga tgaaacggtt ttatgagcag gttgcgcgtt
    12541 ttccggcaga caggatggtt tatcgcgggc ctgatgacgg gcttacccgc cactgccgcg
    12601 cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg tcgccaccac gaccccgccg
    12661 gatgcgacag ccacgttaca ggccatgcag tcctgtcggc gggagtccgc ggcgctggag
    12721 cggcttgact gctatgaccg cctgctggca ccgctgtccc cgtcagggtt tgacggggcg
    12781 ctggtcaaag ccggttttgt gggcgaagcc tggacccgcg ccacggaaca ggaaaaacac
    12841 cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggagcg cccgacggtg
    12901 gtgataacca ccccggctat cggtcatgtt ccgccacgcc cggtgctgat gttcagttgc
    12961 gtggacaata tcacccggat gcaggtggcg ctgatgcacc ctctggatgt ccatgatatc
    13021 gccgtcactc tgaatgccga caaccgggca ttgcgcagcc actggtttgt gcgcgagaac
    13081 ggcaccctgc tggagtccag ccgggggtta tccgggattg atgaaattaa gcaactgttc
    13141 ggcgcaaaaa cgctgacggt ggataccggc gcagacaatg ccgccggaaa gctgaccttt
    13201 aacattgacg ggctggcaag ggcgattgct cccctgcgcg atgcctgcca ctgggcggga
    13261 gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc acctgctgga
    13321 aaacctgccg gaagagaaac tggcatcggc gctgaaagat gacaacccga actgggagta
    13381 tatcgacggc gaaatcgtca agctgggatc gctggcccac agtcaactgg atatcccgga
    13441 actccagcgc cgtgggctgg tcattctggc ttcggagagt aaggattttc gtctgctggc
    13501 gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc gcctgctggc
    13561 gctgtatgtg gagcattact gggcggtggc tgcgccgcag aatatggcgc ataaaaaacg
    13621 ctttgccagc caggtcatta aacgctttga aacaggtatt gaagtttttt cacagaacgc
    13681 cgccacagcg cagcgcgatg cgctgtcagg ggaactggca aaactggcgc aatgctggca
    13741 gtcacataac gccccggaac tggcacaggc tactgatgat ctttttgccc tgtaccagcg
    13801 tgcgtttaat cgtgcggcac cggctcctgt ccccactccg gcggcctccg gtagttcacc
    13861 acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtgctccgg ctccccaaat
    13921 cgtcatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag tggcggccat
    13981 tttatgtgaa cgccagccgg attcaccgca gggctaccgt ctgcgccgcc atgcgctttg
    14041 gcagaacatc accagtacgc cccaggcgga aagcgatggc cgtactccac tggctgcggt
    14101 ctccgccgat atggtggcag attaccatgc tcagctcggt agtgccgata tggcgctgtg
    14161 gcagcaggtg gagaaaagcg tactgctggc tccttactgg ctggatggtc actgcctttc
    14221 tgcacagacg gcgctgcgtc tgggttacaa acaggtggca gacgccatcc gcgatgaggt
    14281 catccgcttc cttgagcgtc tgccccagct taccgggctg ctgtttaatg accacacacc
    14341 atttatcagt gagcagacga aacaatggct ggcagcttcg cccgacgcca aagttgcacc
    14401 ggttgcgcaa atcggtgagg aatcgaaggc agccagagcc tgttttgctg agcagggtct
    14461 ggaggcggcg ctgcgatatc tggacatgct acccgaaggc gatccccgcg atcagtttca
    14521 ccgccagtac cttgccgcac agttgacgga agaggcgggg ctggtacagc ttgcgcagca
    14581 gcagtaccgg atgttgttca ggatgggatt gcagatgatg gtggcagact gggagccgtc
    14641 cctgcttgag cagctagaac aaaaattcac ggcagaacaa taaaagagaa caggagtttt
    14701 tgtgttcaga ttaccaacac cccgactact cagcggactc aaatcagccc tgcgaccggc
    14761 gatgccccgg tttaaagtct ctgctttctg gctgctgata ctggcgtgga tttttctgct
    14821 ggtgtggatc tggtggaaag gcccgacgtg gacgctgtat gaagaacagt ggctcaaacc
    14881 actggcgaac cgctggctgg caacggcggc gtgggggatt attgccctga tgtggcttac
    14941 cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg caaaagcaac agcgcgagga
    15001 agccgttgat ccgctcagcg tggaactgaa cgcccagcag cgttatcttg accgctggct
    15061 gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg tggcagttgc cgtggtatat
    15121 ggtcatcggc ccggcgggca gtggcaaaac gacactgctg cgcgaggggt ttccgtccga
    15181 cattatttat gccccggagg gcgcacgcgg cgcagaacaa cgcctgtacc tcacgcccca
    15241 tgtcggtaaa caggcggtga tctttgatat cgacggcaca ctctgcgctc ccgctgatgc
    15301 ggatatcctg catcgccgtc tgtgggaaca tgccctcggc tggctgaaag aaaagcgcgc
    15361 gcgccagccg ctgaatggga ttattctgac actcgattta cccgatctgc ttaccgcaga
    15421 caaacgccgc cgcgagcatc tgttacaggc gctgcgcagc cgcttgcagg atatacgcca
    15481 gcatcttcac tgccagttac cggtttacgt ggtacttacc cggcttgatt tattgcaggg
    15541 ttttgccgcc ctgtttcagt ccctgaacag acaggatcgc gatgcgattc tgggcgtcac
    15601 gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg ctttctggca
    15661 gacatgggtg gatcgaatga atctggcgtt gccggatctg atggtcgctc agactcacac
    15721 ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga agccgtgaac cgctggtgtc
    15781 actgcttgag ggtctgcttg atggcgaaaa tatgaacgtg atgctgcgtg gtgtctatct
    15841 cacctcttcg cttcagcgtg gacagatgga tgatatattc acccagtctg ccgcccgcca
    15901 gtaccggctg ggcaataacc cactggcgtc ctggcccctg gtggacaccg cgccttattt
    15961 cacccgcagc ctgttcccgc aggcattact cgcagagcct aatctggcaa cagagagccg
    16021 cgcctggctg atacgttccc gtcgccgcct gacggttttc tctgccacag gcggcgtggc
    16081 agcactgctg ctcatcaccg gctggcatca ctattacaac ggtaactatc agtctggcat
    16141 caccgtgctt aagcaggcca aagcctttat ggacgtgccg cctccgcagg gggaagatga
    16201 ctttggcaac ctgcaactgc cgctcctgaa cccggtacgc gatgccacac tggcctatgg
    16261 cgactggggc gaccgcagcc gtctggccga tatgggactg taccagggac gacgtatcgg
    16321 gccttatgtg gaacagacct atctgcaact gctggagcaa cgttacctgc cctcgctgtt
    16381 taacgggctg gtcaaagcgc tgaacgccgc gccgccggag agtgaagaaa aactcgcggt
    16441 gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt aacaatcagg tggtgaagca
    16501 gtatatggca aaacgctgga gcgaaaagtt ccacggtcag cgcgatatcc aggcacaact
    16561 gatgtcccat cttgactacg cgctggctca tactgactgg cacgcagagc gtcaggcggg
    16621 cgacggtgac gccatcagcc gctggacgcc atatgacaag cccgtggtat cagcacagaa
    16681 agaactgagc aaactgcctg tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct
    16741 gggcgttctt cctgccgacc tcaatctgcg tgaccaggta gggccaacct ttgaccaggt
    16801 gtttacatct gccgatgaca acaaactggt tgttccacag tttcttaccc gttacggcct
    16861 gcaaagctat tttgtaaaac agcgcgatga actggttgaa ctgacggcga tggattcctg
    16921 ggtacttaac cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg aaatccagcg
    16981 ccagttgacc gagcagtata tcagcgacta caccgccacc tggcgggccg ggatggacaa
    17041 tctgaatatc cgcaattttg agtccatcgg acaactgacc ggggcgctgg agcaggttat
    17101 cagcggcgac cagcctttgc agcgggcgct gaccgtgctg cgtgacaaca cacagccagg
    17161 cgtcttttct gaaaaactct ctgccaaaga acgggaggaa gccctggcag agccggatta
    17221 ccagttactc acccgcctcg ggcatgaatt cgccccggaa aacagtaccc tggcagtaca
    17281 gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta
    17341 cctgctggca atccagaacg cgcctgtacc agggaaatcg gcgctgaaag ccgtgcagtt
    17401 acggcttgat cagaacagca gcgatccgat attcgccacc cgccagatgg caaaaacgct
    17461 gcctgctccg ctcaaccgct gggttggcag actgactgac caggcctggc atgtggtgat
    17521 ggtggaggct gttcattata tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa
    17581 cgagcaactg gcaaataact atccgtttaa tccgcgttct gcacaggatg cctcactgga
    17641 tgccttcgaa cgcttcttta aaccggatgg catactggat accttctacc agcagaacct
    17701 gaagctgttt atcgataatg acctgagtct ggaggatggc gataacaacg tcattattcg
    17761 cgaagatatt attgcgcaac tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa
    17821 acagaacggt ctgggaacat cctttgccgt ggaaacggta tcgctttcag gcaataaacg
    17881 ccgcagtgta ctgaaccttg acggtcagtt agtcgattac agccagggcc gtaactatac
    17941 cgcccatctg gtctggccta acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat
    18001 cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg cgcagttccg
    18061 cctgttcggg gccggacaac tgaccggagt acaggatggc aactttaccg tgcgctttag
    18121 cgtggacggt ggcgcgatga cctaccgtgt gcataccgac acggaagata acccgttcag
    18181 cggtgggttg ttcagccagt ttggtctgtc agacacactg tactgaggag gcagggatga
    18241 atagtaacgt actgacacaa actatcgtta ccggcagtga cccgcgcggg ctgccggaat
    18301 tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct gagctgaact
    18361 ggaaactggt ggagtcgctg gcactggcga tttttaaagc caacggtgtg gatttacaca
    18421 ccgccaccta ctatacgctt gcccggacac ggacacaggg actggcggga ttctgcgaag
    18481 gtgcggaact gctggcggca atggtaagcc acgactggga taagttctgg ccgcagggcg
    18541 gcccggcgcg tactgaaatg ctggactggt ttaactcccg aaccggcaat attctgcgtc
    18601 agcaaatctc ctttgcggaa tccgacctac cactgatcta ccgcacagag cgggcattgc
    18661 agcttatctg cgataagctc cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc
    18721 tgctctattt tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag agtaacactg
    18781 agaacgccgc acagaccacg gtcagaacgc tgatttatgc cccggaaaca caggcatctt
    18841 ccacaccaga agcggtagtg cctcccctgc ccggcctgcc tgagatgaaa gtggaagtgc
    18901 gcagtctgac agagaatccc ccacaggcca gtgtgataaa gcaaggcagt acggtaagag
    18961 ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg
    19021 tctatccggt acagcagcaa ctgttacagg ttaacgacac cgctcagggc gcagcaacgg
    19081 tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa cttcttgata
    19141 cctccccggt acagccgctg gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct
    19201 ggccggaaag cctgcaacag caacaggcct cgacacaatg gaatgaggca ctcaaaaccc
    19261 gcgcacagag tagcccgcag ttgcgtggct ggttgcagac ccgccaggac ttacatgctt
    19321 ttgcagatct agtgatgcag cgcgagaaag agggactaac cctttcctat atcaaaaatg
    19381 tcatctggca ggcggagcgg ggactggggc aggaaacacc cgttgagtct ctgttgacgc
    19441 agtaccagga tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa caaattaatg
    19501 agcgactcga aggcgtgtta agccgctggc tgctgctgaa gaataacgtc atgccagagg
    19561 cggcaaccgg taccacggcc gaaaaataat tatttaatta aaggagatta tcatggcaaa
    19621 tataagttat ttatctttga gtggagagac acaagggctg atttcagcag gttgctcaac
    19681 gcttgattct gtcggaaaca aagcgcagcc agaacataaa gatcagatca tggtttatgc
    19741 gttaatgcat tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa
    19801 accggttgat aaatcc
//
LOCUS       DAFAVF010000072        19756 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFAVF010000072 DAFAVF010000000
VERSION     DAFAVF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.072"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(398..1594)
                     /gene="hemY"
                     /locus_tag="LGK42_002582"
     CDS             complement(398..1594)
                     /gene="hemY"
                     /locus_tag="LGK42_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBK2658818.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1597..2838)
                     /gene="hemX"
                     /locus_tag="LGK42_002583"
     CDS             complement(1597..2838)
                     /gene="hemX"
                     /locus_tag="LGK42_002583"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBK2658819.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2860..3600)
                     /gene="hemD"
                     /locus_tag="LGK42_002584"
     CDS             complement(2860..3600)
                     /gene="hemD"
                     /locus_tag="LGK42_002584"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBK2658820.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3597..4538)
                     /gene="hemC"
                     /locus_tag="LGK42_002585"
     CDS             complement(3597..4538)
                     /gene="hemC"
                     /locus_tag="LGK42_002585"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBK2658821.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4925..7471
                     /gene="cyaA"
                     /locus_tag="LGK42_002586"
     CDS             4925..7471
                     /gene="cyaA"
                     /locus_tag="LGK42_002586"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBK2658822.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7511..7831)
                     /gene="cyaY"
                     /locus_tag="LGK42_002587"
     CDS             complement(7511..7831)
                     /gene="cyaY"
                     /locus_tag="LGK42_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBK2658823.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7879..9204)
                     /locus_tag="LGK42_002588"
     CDS             complement(7879..9204)
                     /locus_tag="LGK42_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBK2658824.1"
                     /translation="MRRQNEGSNNQVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGK
                     GYWGDKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMYRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIP
                     LDTPDLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9188..10666)
                     /locus_tag="LGK42_002589"
     CDS             complement(9188..10666)
                     /locus_tag="LGK42_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBK2658825.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKRTDNNGGGNRL
                     DIKTLHQVEFRPPRDPDLLGLILRDANFLYINGHRLADPDSFRVLAQKSWDQRGKFST
                     AFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11034..11588)
                     /locus_tag="LGK42_002590"
     CDS             complement(11034..11588)
                     /locus_tag="LGK42_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HBK2658826.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11938..12141
                     /locus_tag="LGK42_002591"
     CDS             11938..12141
                     /locus_tag="LGK42_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2658827.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12178..13002
                     /gene="dapF"
                     /locus_tag="LGK42_002592"
     CDS             12178..13002
                     /gene="dapF"
                     /locus_tag="LGK42_002592"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK2658828.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12999..13706
                     /locus_tag="LGK42_002593"
     CDS             12999..13706
                     /locus_tag="LGK42_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBK2658829.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13703..14599
                     /gene="xerC"
                     /locus_tag="LGK42_002594"
     CDS             13703..14599
                     /gene="xerC"
                     /locus_tag="LGK42_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK2658830.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14599..15315
                     /gene="yigB"
                     /locus_tag="LGK42_002595"
     CDS             14599..15315
                     /gene="yigB"
                     /locus_tag="LGK42_002595"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBK2658831.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15399..17561
                     /gene="uvrD"
                     /locus_tag="LGK42_002596"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15399..17561
                     /gene="uvrD"
                     /locus_tag="LGK42_002596"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBK2658832.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17708..18472)
                     /locus_tag="LGK42_002597"
     CDS             complement(17708..18472)
                     /locus_tag="LGK42_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBK2658833.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18842..>19756
                     /gene="corA"
                     /locus_tag="LGK42_002598"
     CDS             18842..>19756
                     /gene="corA"
                     /locus_tag="LGK42_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBK2658834.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLA"
BASE COUNT         4592 a         5143 c         5170 g         4851 t
ORIGIN      
        1 cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa gttattactc
       61 aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg ttatttcccg
      121 attctctgtg gcataataaa cgggtagatg ctcattccat ctcttatgtt cgccttagtg
      181 cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca ctgacagatg
      241 tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc acttgttgtc
      301 atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt atgcatcaga
      361 aaggtcccgg aggtgcttgc ctccgggtga gaaggaacta ctgtggtggg ttgttctgca
      421 acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg tgcagtctgt
      481 ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc agcgccgcgc
      541 ggaaggcgag cgatgcttcc tgccattctc cgtgcttcat cagtgactgg cccagtgtgc
      601 tccacaacag cgggcgatcg ccgacgtttt tgatctgctg gcgcagcact ttttccagct
      661 gttccggatt gttggttttc agtcgaggaa tcggcagcag taggcgatcg tcgtactggc
      721 gtttcaggcc atcgatgata atttgctggg cagtatcatg atcgtcacat tcaataagat
      781 gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt tggtttttcc
      841 accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc aggccaatcc
      901 atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct ttcgccattg
      961 atgggatgat atccagcaga gaactccatg cacctgtgcg gatatacgcc tgttccgcca
     1021 gacgtaatac ttccggatgg cgtggcgtaa cttccagcag cttatccacg ccgtggcgtg
     1081 cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct accggaatgg
     1141 tgtcattgcc ggccagctct gctgcgcgtt ccagatgttg gttggcgcgt gcttcatcac
     1201 cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt tccgcgtgat
     1261 cggcattttt cgccatcagc ttttcaactt gctgataatc gccttccgcc agtttcagca
     1321 gcgcctgttc ggtctgctta cgggcacggc gacgcttacg tccgacaaac cacccacggg
     1381 tgtgtgcgcc tgtgcggaag atccgccgca gtagccactc aatggcaaac agcactacca
     1441 tcgccagaat caaaataatc gccaggcccg tgacgctggt ttcgatattg tagttgtcag
     1501 tctggatcag cacgtaaccc tgatgaccgg caatcatcgg gccaaccact atccccgcaa
     1561 tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg gcgcggctgc
     1621 cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg
     1681 tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc gttgtccccg ctgccggttg
     1741 tgccagcagg ttacgcacgc gggtctgcat cagtttttcc agcatcgcct ggctttgcag
     1801 ggtttccgga agatccatcg agatattttg ctggcttaac tggtccacct cgtcgaggaa
     1861 cgctttggtg gtggcatcat cagtatcgta gtaagctcgt acccaggtgg agacgttctc
     1921 cagcgcctgg cgataagtct cttcctggtg acgcggtaca gcttgtgctg cgaccagcag
     1981 gcgagagcga atattttcgc gcagatagat atcctgattt ggcgctaaca gcggtacggc
     2041 ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata aagttctgcc agcttttttg
     2101 cagattgata cgccattcgc tgatggaact ggaaagctct tcaccgtcgg aatccatcgg
     2161 cgaaccatcg ctgtcattat cggccagacg caggttatct acctgatttg aaagctgatt
     2221 aagcttaagg atgatgccgt cataatccac ctgcgaaact gcagaaaggc tggcgatatc
     2281 atcggtaatt gcccgacgaa cggtaatcag actcgggtca ttcatatccg ccaggctggc
     2341 atctgcactt ttcagcaacg ctgcagcggt cgtgacgtcc tgatcgctcc acagcttccg
     2401 tccggcgagt ttcaccagaa aatcggcctg agccagcagc caggttttag catcgctgcc
     2461 ggaaatggtg gcgacctttt gttggacttc atccaactgt tttgccagcg tttcttgctg
     2521 acgattcgcc tgcttaagct gtgcagcttg ttgcttaata atgccttcca gctcggcttt
     2581 ttggctctcc tgggcttttt gcaatgccgt cagttggtta gccagggcat cgctggtggc
     2641 ggtctgattg acggcctgtt gtttacccca gccatacaaa ccgacgcccg ccgccagagc
     2701 aatagcgata gccaccgcgc tgagaatcaa tgcggtattg ttcttactct ttttttctgt
     2761 tgcgacaggt tgtgacgtgg tgtccacggc ctccctggtc tcttcaacca cggcggaggt
     2821 tttttcttgt tccgtcatta tggcttcctg ttatgagagt tattgtaatg cccgtaaaag
     2881 cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc cagcccagtt cccgggcgag
     2941 tttcgccaaa cgctcactga cgaccaatag tcgacagtgt agtaaccagt gctcacgata
     3001 ccattgtggg atcagcgacc agagttgctg caacatttca ccgctggtaa caacgaccat
     3061 cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca ccatcgtaat ggattgcgca
     3121 tcgttgataa cattcacaaa aagtgacctc agcaccgcgc gctgtcaggg tatccccaat
     3181 tagctcacga ccgccattgc cacgtaatat cagcgcacgt ttgcccgcaa tattttgtaa
     3241 ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc tgcgggtaga gaatcttctg
     3301 tccgcttacg gtatgtagcg ccagtgcggt ggtgcgtcca atggcgaaat aatcaggtag
     3361 tcggggccat ttacgatctt gctgatgcag ctgtgattgg gcaaaagcaa ccgcgtgttg
     3421 cgagagggca aacaacagat cgctctcccc cagcgccgcc agttgatcag caagttgcgg
     3481 taattgtcga cccggagaaa actcaatcag cggaaaatgc caggccacct gccccagtgt
     3541 gcgcagacgg ctcactaact cttctccagc gggagacggg cgggtgacca ggatactcat
     3601 gccggggcgt ctccgttata gacttcagcg aggatctcgc gcgcgccgtt attcagtagc
     3661 tcttctgcca gcgaaatccc catttgttcg gcattttgcg gcgcaccgcg gcgttcaccg
     3721 cgaataatct gcgaaccgtc cggcgcgccg accagcgcac gcagccagat ttcaccatca
     3781 ataagctcgg cgtagctacc aattggcacc tgacatccac cttcgagacg ggtattcatg
     3841 gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat tcagcgcggc aagcagctcg
     3901 cgagtgcgcg tatcatcaag gcggcattca ataccgactg caccttgtcc taccgccgga
     3961 agagaaatct cgggtggtaa cgcggcgcga atgcgggact ccagacctaa acgttttagt
     4021 ccggctacgg caagaatgat ggcatcgtat tcgccgttat ctagtttgct caggcgagtg
     4081 ccgacgttgc cgcgcaggga gcggataatc agatccgggc ggcgttcagc cagttggcac
     4141 tggcgacgta aactggacgt cccgacgata ctgcctgccg gtaacgcatc cagactgtca
     4201 taattattgg acacaaaggc atcgcgagga tcttcacgct cacaaatagt gaccaacccc
     4261 agaccttgcg ggaattcaac cggcacatct ttcattgaat gtacggcgat atcggcgcga
     4321 ttctccagga gcgcgacttc cagctctttt acaaataagc cttttccgcc tactttcgcc
     4381 agcggcgtat caagaatcac atcgccgcgc gtcaccatcg gtaccagttc aaccaccagg
     4441 cccggatggc tcgccatcaa cttgtctttg acatagtgtg cctgccagag tgcaagtggg
     4501 ctttggcgtg tggcaattct taaaacattg tctaacatgc ttgttaccgt cattatcatc
     4561 cgtggtccat cctaacatcc ttgccagagt gatgtcagtg ttgtggtgaa acgtagacgc
     4621 ctgcgcaaat cgtaaaatga ggtctggcag tggatcctga caggcgtttc acgccgtcgt
     4681 aataaggaat ttacagagaa taaacggtgc tacacttgta tgtagcgcat ctttctttac
     4741 ggtcaatcag caaggtgtta aattgatcac gttttagacc attttttcgt cgtgaaacta
     4801 aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac atacgaaata tcccgaatgc
     4861 cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac gggtagcaaa tcaggcgata
     4921 cgtcttgtac ctctatattg agactctgaa acagagactg gatgccataa atcaattgcg
     4981 tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa caggtctaca gtctactgcc
     5041 gacattgttg cactatcacc atccgctaat gccgggttac cttgatggta acgttcccaa
     5101 aggcatttgc ctttacacgc ctgatgaaac tcaacgccac tacctgaacg agcttgaact
     5161 gtatcgtgga atgtcagtac aggacccacc gaaaggtgag cttccaatta ctggtgtata
     5221 caccatgggc agcacctcgt ccgtagggca aagttgttcc tctgacctgg atatctgggt
     5281 ctgtcatcaa tcctggctcg atagcgaaga gcgccagtta ctacaacgta aatgtagtct
     5341 gctggaaagc tgggccgcct cgctgggtgt ggaagtcagc ttcttcctga ttgatgaaaa
     5401 ccgcttccgt cataatgaaa gcggcagcct ggggggcgaa gattgtggct ccacccagca
     5461 tatactgctg cttgacgaat tttatcgtac cgccgtgcgt ctcgccggta agcgtattct
     5521 gtggaatatg gtgccgtgcg acgaagaaga gcattacgac gactatgtga tgacgcttta
     5581 cgcgcagggc gtgctgacgc caaatgaatg gctggatctc ggtggcttaa gctcgctttc
     5641 tgctgaagag tactttggtg ccagcctttg gcagctctac aagagtatcg attccccata
     5701 caaagcggta ctgaaaacac tgctgctgga agcctattcc tgggaatacc cgaacccacg
     5761 tctgctggcg aaagatatca aacagcgttt gcacgacggc gagattgtat cgtttggtct
     5821 cgatccatac tgcatgatgc tggagcgtgt tactgaatac ctgacggcga ttgaagattt
     5881 tacccgtctg gatttagtac gtcgctgctt ctatttaaaa gtgtgcgaaa agctcagccg
     5941 tgaacgcgcc tgtgtaggct ggcgtcgcgc agtgttgagc cagttagtga gcgagtgggg
     6001 ttgggacgaa gctcgtctgg caatgctcga taaccgcgct aactggaaga ttgatcaggt
     6061 gcgtgaggcg cacaacgagt tgctcgacgc gatgatgcag agctaccgta atctgatccg
     6121 ctttgcgcgt cgcaataacc ttagcgtctc cgccagtccg caggatatcg gcgtgctgac
     6181 gcgtaagctg tatgccgcgt ttgaagcatt accaggtaaa gtgacgctgg taaacccgca
     6241 gatttcaccc gatctctcgg aaccgaatct gacctttatt tatgtgccgc cgggccgtgc
     6301 taaccgttca ggttggtatc tgtataaccg cgcgccaaat attgagtcga tcatcagcca
     6361 tcagccgctg gaatataacc gttacctgaa caaactggtg gcgtgggcat ggtttaacgg
     6421 cctgctgacc tcgcgcaccc gtttgtatat taaaggtaac ggcattgtcg atttgcctaa
     6481 gttgcaggag atggtcgccg acgtgtcgca ccatttcccg ctgcgcttac ctgcaccgac
     6541 accgaaggcg ctctacagcc cgtgtgagat ccgccatctg gcgattatcg ttaacctgga
     6601 atatgacccg acagcggcgt tccgcaatca ggtggtgcat tttgatttcc gtaagctgga
     6661 tgtcttcagc tttggcgaga atcaaaattg cctggtaggt agcgttgacc tgctgtaccg
     6721 caactcgtgg aacgaagtgc gtacgctgca cttcaacggc gagcaatcga tgatcgaagc
     6781 cctgaaaact attctcggca aaatgcatca ggacgccgca ccgccagata gcgtggaagt
     6841 cttctgttat agccagcatc tgcgcgggtt aatccgtact cgcgtgcagc aactggtttc
     6901 tgagtgtatt gaactgcgcc tttccagcac ccgccaggaa accgggcgtt tcaaggcgct
     6961 gcgcgtttct ggtcaaacct ggggcttgtt cttcgaacgc ctgaatgtat cggtacagaa
     7021 gctggagaac gccatcgagt tttatggcgc tatttcgcat aacaaactgc acggcctgtc
     7081 agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg gtggacggct ttgctagtga
     7141 agggatcatc cagttcttct tcgaagaaac gcaagacgag aatggcttta atatctacat
     7201 tctcgacgaa agcaaccggg ttgaggtgta tcaccactgc gaaggcagca aagaggaact
     7261 ggtgcgtgac gtcagtcgct tctactcgtc atcgcacgac cgcttcacct acggctcaag
     7321 cttcatcaac ttcaacctgc cgcagttcta tcagattgtg aaggttgatg gtcgtgaaca
     7381 ggtgattccg ttccgcacaa aatctatcgg taacatgccg cctgccaatc aggatcacga
     7441 tacgccgcta ttacagcaat atttttcgtg atgaacgtgc cggaaagcga ggcttatccg
     7501 gcatgcaatc ttagcggaaa ctgactgttt cacccgcctg ctgtgtcgcc gcctgttcca
     7561 gcaaatccca gaaggtttcg ccgctgcgat cgcaaatcca ctcatcgcct ttcagatcaa
     7621 aatggtagcc gccttgtttg gttgccagcc atacctggtg cagcggttcc tggcggttga
     7681 taatgatttt gctgccgttt tcaaaggtaa tggtcagcac gccgccgttg atttcgcagt
     7741 cgatatcgct gtcgccatcc cagtcgtcca gacgctcttc aatggtcagc cacagttgat
     7801 cagccaggcg atgaaattca ctgtcgttca ttgttgtatc ctgtttttaa gtgatggcgg
     7861 cagtatagcg gcatggggtc agggcttcaa agtttgcacc tctgctgctg cgttccggca
     7921 tgattcatcc gtcaccggaa taatgatgtc tctgttttga acttcacgta cgttctcctc
     7981 tctgacacga gttttcgccg ggcaggggga gggcgtaatc cttacctctg tatcggtcca
     8041 tgtataaaca ttgcgatcgt cataagcgaa gcgatcgttt ttagttagcg gaccgttaac
     8101 tggaataatt tgcaagctct ttgaccgtaa ttggcggata aagtaagcat gttgtttgcc
     8161 gccattaaat gagcgatcaa tgaccaggtc tggcgtatcc agcgggatta tctcaagctg
     8221 gtcttcacca aatatcgata attttgttac atacagccga tctttatatt gcgcaatatt
     8281 gccagtaatc agatgaaatt gttcaggttc aatacctggt aggtctatcc acttgctcca
     8341 ctgacggtcg gacgtgagct gtttacgcca gcgaacgcga ctttccccta cttcaaaaga
     8401 gacgtcatca tctacgggta tcgctttgcc tgataattga ccaaagggat aacgatacat
     8461 acccttattg tcgcgaaaaa ccagcagtga atgaggtatc cagcgaatta tctcaaatga
     8521 gtctgcattc gccgtaaggt gaacgccatt taaataaata ttctgcccac tgcggataag
     8581 cagatcattg gaatacaaat ttctgtttac actcataaga cgtggattaa taccccacca
     8641 tcttttttct aatacagtaa cattactgct gctcccctga cgtcgaccgg acaaatagag
     8701 atttttacta tccaccagcg tggtagagtt accttcgata gcttttagtg tcgccagatc
     8761 aacacgtgac gcgccgctat tactttcggt gcgctgtccg tcaaaataga ggctatattt
     8821 atcgaccgcg aagcggccca tagcctgaaa gctggcaata tcgacagtgg gggttcccgg
     8881 tggattgcta acgattttgc cacgccagag aataacgcgg ccgtctgtca cccagtggta
     8941 ttcctcattt tttatgctta tttcatcgga aggtatataa cccgttttcc cagagatatt
     9001 tttcacaagg cgcaaatcgg ccctgctcat ctccggaaac tgggcaatga ttttcttatc
     9061 cccccaataa cctttaccgt aagacataac accgtgatca ttgtaatagt cgaaccctat
     9121 ggcctgtaca gcaggcgtca gaaatgtgtg tcccaacagg atgagtgcaa cttgattatt
     9181 tgacccttca ttttgtcgcc tcattgcgcg gggcaggaat aaattcgccg tcatcagtaa
     9241 tttttccgtc cagatatccc gttttgtaat ttacgccata gccatatccc gaatgttgtt
     9301 ccttccagat taagcgaact tgtttcggat cagcggtgcg gtatcgccag agctgcgcgc
     9361 catcaaagct gtaaatgtag cggttatcgc gggcatacca tctgcgcaaa aagggatggt
     9421 cattaagatg ggcttcgcgt tcatccggag aacggttatc cagtaagatc agcggtccgt
     9481 cagattcaaa cacatgtaaa ccggaatggt atggccaccc ttcggcacgc gtaagtaaat
     9541 agccgtggcg tttcccagcg ttgaaacgtt tgttgataat aagattggga tcatcaagtt
     9601 tcacgatttc cagcttgcgg tcaccagact ctgtttcgat gatggtataa agcgagtcct
     9661 gatactgggc gacagcatca ctgatgggtt gaaactgctc cggatcgagt ccgggtaatt
     9721 cttcctgttt acaagcaggt cctttacgcc agaacacttt cttttccagc agagaaaaat
     9781 ccagacagtg ctttgttaaa tcttcatccc acgccagatt ttccttgtcg aggactttac
     9841 gctgcagccc gtttttatca cgccaggtca agagtgagcc gggcatccag cgcacgacgg
     9901 aaaaggtgtc cgggtcggca tcaagctgtt cgccgttgat caggattttg gtccgtgtac
     9961 gagccagggt atcccagggg ccaaatggca tagccacaca gggattaaac gcggtagaaa
    10021 actttcctcg ctgatcccat gacttttgtg ccagcacacg gaaactatcg ggatctgcga
    10081 ggcgatgccc attgatatac aagaaattag cgtcacgtaa tatcagcccc agtaagtcag
    10141 gatcccgggg aggccggaat tccacctgat gcagtgtttt gatatctaag cgatttcccc
    10201 cgccattgtt atctgtgcgc ttgccttcga aatagagact gtgtttatct gcggcaaaat
    10261 caccccacgc cttgaacgag gcgacatcga cgggaggttt gtctggcggg ttttgcacaa
    10321 cttcacccgc gtagaggata tagcgaccat cgctatgcca ggagtaatga ggataccagg
    10381 cctgtgcgcc ttcatagagg tagtcggttg tcagatcagt cagaggaggt actttatccc
    10441 atttgtctag tgttgtcggg tctattaata aattgggtaa acggcgcaaa cttttgtaat
    10501 tcacgtcagg aagagaaaga tttgtttctt tataagggcg caggtatacc acgccttttc
    10561 cctgtttctg gtaaatatta tgttcaggcg gatcaagccg acatccccat gccgacgata
    10621 aaaacaatga acaagacaac aaacaaacca cattgcgata gtgcataaag ccatcctggc
    10681 gcgaggtgcc gatcacgaaa ctaccagcag aacataattc ctcgcacgta agcgtaactt
    10741 ttgcggcaaa tcgtcacttt tcagaatcta taaaggccat cgctattttc tgatagttgc
    10801 tggggtaaac ttgaggtttt cattcatgat ataaatacga gctactgcgc catggtgtga
    10861 ctgatcacgt taaaatgtgc cggattgacg ccgggaaggg tgtcaacatg cgactctgcc
    10921 caccgttacc tactttattg tctttcgcgt gttgtactta ggtcccacca ccggtatcta
    10981 acattcccca cattattacg ctttctgccg aacgtgattt ttatagaaat ctatcaattc
    11041 attttccctg taccataaag tttatctttc acatcctgcc gaacgcgggt cttgtacccc
    11101 ccgttgtgaa gatcttcata cggagaaaaa gaatctttac tccaccccag cagcacttta
    11161 tcgccaggca taaaatgcac atgcagaaaa tcttctcccc attctctttt ggggagaggt
    11221 aacgtcatat tccgtttctc cagacgcatt ccagcattat actgtgtacg agtagtactt
    11281 aatgtccaga taacctctgc cgtatcccca ctgacatcac cacaacaggt gattgaaccg
    11341 ccaccatttg caaaagcatt cgcgcccgcc acgccgttaa cactgaatcc cagtatcgga
    11401 cggtcaattt cactgtggat ttccagaatc actccacctc ttggtcctcc ccatactgcc
    11461 tgtcctatat tccacaacac gaccagtacc ggaacgacaa ttaacgtccg ccatacccag
    11521 cgcccccagc gggcataacc gcgcgtaagc ttttggtcaa atttttccag ttgggtaaat
    11581 agccccatca gattttattc cctcttatca ccaggttggc aaagttgtat gtcactggcg
    11641 actaaggtca gacaacgaaa ttgcatggtg cgccactcta ttaaaagccc catgccgacg
    11701 ataagaacaa tgaacaagac aacaaacaaa ccacattgcg atagtgcata aagccatcct
    11761 ggcgcgaggt gccgatcacg aaactaccag caaaacataa atccccacgt gtaagcgtta
    11821 tactcgcagc atttcctcac ttttcagact tcataaagag tcgctaaacg cttgctttta
    11881 cgccttctcc tgcgatgata gaaagcagaa agcgatgaac tttacaggca atccataatg
    11941 aaaaacgtgt ttaaggcact cactgtatta attactctct tcagcctgac gggctgcggt
    12001 ctgaaaggtc cgctctattt cccgcctgca gataaaaacg caccgccgcc gaccaaaccg
    12061 gtagagacgc aaacgcaatc cacggtgccg gataaaaacg atcgcgccac tggcgatggt
    12121 ccatcccagg tgaattacta aaagtcagtt tctgtacccg cgtgattgga gtaaatgatg
    12181 cagttctcga aaatgcatgg ccttggcaac gattttatgg tcgtcgacgc ggtaacgcag
    12241 aatgtctttt tttcaccgga gctgattcgt cgcctggctg atcggcacct gggagtaggg
    12301 tttgaccaac tgctggtggt tgagccgccg tatgatcctg aactggattt tcactatcgc
    12361 attttcaatg ctgatggcag tgaagtggcg cagtgcggca atggtgcgcg ctgctttgcc
    12421 cgttttgtgc gtctgaaagg gctgaccaat aagcgcgata tccgcgtcag caccgccaac
    12481 gggcggatgg ttctgaccgt caccgatgat gatctggtcc gcgtaaatat gggcgaaccc
    12541 aacttcgaac cttccgccgt gccgtttcgc gctaacaaag cggaaaagac ctatattatg
    12601 cgcgccgccg agcagacaat cttatgcggc gtggtgtcga tgggaaatcc gcattgcgtg
    12661 attcaggtcg atgatgtcga taccgcggcg gtagaaacgc ttggtcctgt tctggaaagc
    12721 cacgagcgtt ttccggagcg agccaatatc ggttttatgc aagtggttaa gcgcgagcat
    12781 attcgtttac gcgtttatga gcgtggggca ggagaaaccc aggcctgcgg cagcggcgcg
    12841 tgtgcggcgg ttgcagtagg gattcagcaa ggtttgctgg ccgaagaagt acgcgtggaa
    12901 ctccccggcg gtcgtcttga tatcgcctgg aaaggtccgg gtcacccgtt atatatgact
    12961 ggcccggcgg tacatgtcta cgacggattt attcatctat gaagcaacca ggggaagaac
    13021 tgcaggaaac actcacggag cttgatgacc gggcggttgt cgattatctg attaaaaatc
    13081 ctgagttttt tatccgtaat gcgcgcgcag tagaagcgat acgtgtgccg catccggtac
    13141 gcggcaccgt ttcgttggtc gagtggcaca tggcccgcgc acgtaatcat attcatgttc
    13201 tggaagagaa catggcgctg ttgatggaac aggctatcgc caacgaaggc ctgttttatc
    13261 gcctactcta cctgcagcgc agtctcaccg ccgccagcag tctcgacgat atgctgatgc
    13321 gctttcaccg ctgggcgcgc gatctcggcc tggcaggtgc gagtctgcgc ctgtttccgg
    13381 atcgctggcg cttaggtgcg ccgtcgaacc acactcatct ggcattaagc cgtcagtctt
    13441 tcgaaccgct gcgtattcag cgtttggggc aggaacagca ctatcttggg ccgcttaacg
    13501 gaccagagct gctggtggtg ctaccggaag cgaaagcggt gggatcggtg gcgatgtcga
    13561 tgctgggaag cgatgctgat ttgggtgtcg tgctgtttac cagtcgcgat gccagtcact
    13621 atcaacaagg gcaaggaacg cagttacttc atgaaattgc gctgatgttg ccggagcttc
    13681 tggagcgttg gattgaacgc gtatgaccga tttacacacc gatgtagaac gctacctacg
    13741 ttatctgagc gtggagcgcc agcttagccc gataaccctg cttaactacc agcgtcagct
    13801 tgaggcgatc atcaattttg ccagcgaaaa cggcctgcaa agctggcagc aatgtgatgt
    13861 gacgatggtg cgcaattttg ctgtacgcag tcgccgtaaa gggctgggag cagcaagcct
    13921 cgcgttacgg ctttctgcgc tacgtagctt ttttgactgg ctggtcagcc agaacgaact
    13981 caaagctaac ccggcgaaag gagtttcggc accgaaagcg ccgcgtcatc tgccgaaaaa
    14041 catcgacgtc gacgatatga atcggctgct ggatattgat atcaacgatc ccctcgctgt
    14101 acgcgaccgt gcaatgctgg aagtgatgta cggcgcgggt ctgcgtcttt ccgagctggt
    14161 ggggctggat atcaaacacc tcgacctgga gtctggtgaa gtgtgggtaa tggggaaagg
    14221 cagcaaagag cgccgcctgc cgattggtcg caacgctgtg gcgtggattg agcactggct
    14281 tgatttgcgc gacctgtttg gtagcgaaga cgacgcgctt tttctgtcga aactgggcaa
    14341 gcgtatctcc gcgcgtaatg tgcagaaacg ctttgccgaa tggggcataa aacaagggct
    14401 gaataatcac gttcacccac ataaattacg tcactcgttc gctacgcata tgctggagtc
    14461 gagcggcgat cttcgtggtg tgcaggagct gctgggtcat gccaacctct ccaccacgca
    14521 aatctatact catcttgatt ttcaacacct tgcctcggtg tacgatgcgg cgcatccacg
    14581 cgccaaacgg gggaaataat gcgtttttac cggcctttgg ggcgcatctc ggcgctcacc
    14641 tttgacctgg atgataccct ttacgataac cgtccggtga ttttgcgcac cgagcgagag
    14701 gcgcttacct ttgtgcaaaa ttatcatccg gcgctgcgca gcttccagaa tgaagatctg
    14761 caacgcctgc gccaggcggt acgggaagcg gaacccgaga tttatcacga cgtgacgcgt
    14821 tggcgttttc gttcgattga acaagcgatg ctcgacgccg ggctgagtgc cgaagaagcc
    14881 agtgcaggcg cacacgcagc aatgattaac tttgccaaat ggcgcagccg aatcgacgtc
    14941 ccgcagcaaa ctcacgacac cttaaaacag ctggcgaaga aatggccgct ggtggcgatc
    15001 accaacggta acgcccagcc ggaactgttt ggtttggggg attattttga gtttgtgctg
    15061 cgcgctggcc cgcacgggcg ctcaaaaccg ttcagcgata tgtacttttt ggctgcggaa
    15121 aaactcaacg tgccgatcgg cgagatctta catgttgggg acgatctcac cactgacgtg
    15181 ggtggggcaa ttcgcagcgg aatgcaggct tgttggatca gaccggaaaa tggcgatctg
    15241 atgcaaacct gggacagccg tttactgccg catctggaaa tttcccggtt ggcatctctg
    15301 acctcgctga tataatcagc aaatctgtat atatacccag ctttttggcg gagggcgttg
    15361 cgcttctccg cccaacctat ttttacgcgg cggtgccaat ggacgtttct tacctgctcg
    15421 acagccttaa tgacaaacag cgcgaagcgg tggccgcgcc acgcagcaac cttctggtgc
    15481 tggcgggcgc gggcagtggt aagacgcgcg tactggtgca tcgtatcgcc tggctgatga
    15541 gcgtggaaaa ctgctcgcca tactcgatta tggcggtgac gtttaccaac aaagcggcgg
    15601 cggagatgcg tcatcgtatc gggcaactga tgggcaccag ccagggcggc atgtgggtcg
    15661 gcaccttcca cgggctggcg caccgcctgc tgcgtgcgca ccatatggac gccaatctgc
    15721 cgcaggattt ccagatcctc gatagcgagg accagctgcg cctgcttaag cgtctgatca
    15781 aagccatgaa cctcgacgag aagcagtggc cgccgcgcca ggcaatgtgg tacatcaaca
    15841 gccagaaaga tgaaggcctg cgtccgcatc atattcaaag ctacggtaat ccggtggagc
    15901 agacctggca gaaggtgtat caggcgtatc aggaagcgtg tgaccgcgcg ggcctggtgg
    15961 acttcgccga gctgctgctg cgcgctcacg aactgtggct taacaagccg catatcctac
    16021 aacactaccg cgaacgtttt accaatatcc tggtggacga attccaggat accaacaaca
    16081 ttcagtacgc gtggatccgc ctgctggcgg gcgacaccgg caaagtgatg atcgtcggtg
    16141 atgacgacca gtcaatctac ggctggcgcg gggcgcaggt ggagaatatt cagcgtttcc
    16201 ttaatgattt ccccggtgcc gaaactattc gtctggagca aaactaccgc tctaccagca
    16261 atattctgag cgccgctaac gccctgattg aaaacaataa cgggcgtctg ggtaaaaaac
    16321 tgtggaccga tggcgcggac ggtgagccta tttccctcta ttgcgctttt aacgaactcg
    16381 atgaagcgcg ttttgtggtt aaccgcatca aaacctggca ggacaacggc ggggcgcttg
    16441 ccgagtgcgc cattctctac cgcagtaacg cccagtcgcg tgtactggaa gaagcactgt
    16501 tgcaggccag tatgccgtac cgtatttacg gcgggatgcg cttcttcgaa cgccaggaaa
    16561 tcaaagacgc gctctcgtat ctgcgtctga ttgccaaccg taacgacgac gcggcgtttg
    16621 agcgcgtggt gaatacgcca acgcggggta ttggtgaccg gacgctggac gtggtacgtc
    16681 agacatcgcg cgatcgccag ttaacactct ggcaggcatg tcgtgaactg ttgcaggaaa
    16741 aagccctcgc cgggcgagct gccagcgcct tgcagcgatt tatggaatta atcgatgcct
    16801 tagcgcaaga aactgccgat atgccgctgc atgtacagac tgaccgggta attaaagact
    16861 ccggcctgcg caccatgtac gagcaggaga agggcgaaaa aggccagacg cgtatcgaaa
    16921 acttagagga actggtgacg gcaacgcgcc agttcagcta caacgaagaa gatgaagatt
    16981 taatgccgct gcaggcattc ctctcccatg cggcgctgga agcgggcgag gggcaggcgg
    17041 atacctggca ggatgcagtg cagttgatga cgctgcactc ggcgaaaggc ctggagttcc
    17101 cgcaggtgtt tattgtcggt atggaagagg gtatgttccc aagccagatg tcgctggatg
    17161 aaggcgggcg tctggaagaa gaacgccgtc tggcctacgt tggtgtaacc cgcgcgatgc
    17221 agaaactgac gctgacctac gcggaaaccc gccgtctgta tggtaaagag gtttaccatc
    17281 gcccgtcgcg ctttatcggc gagctgccgg aagagtgtgt ggaagaggtg cgcctgcgcg
    17341 ctacggtaag tcgtccggtc agccatcaac ggatggggac gccgatggtc gagaacgaca
    17401 gcggctacaa gctcggtcag cgtgtacgcc acgctaagtt tggtgaaggc accatcgtca
    17461 atatggaagg cagcggcgag catagccgtt tgcaggtggc attccagggc cagggtatta
    17521 aatggctggt ggcggcatac gcccggctgg agtcggtgta acgttgccgg atgcggtgct
    17581 gcgcacctta tttggcctaa aaaatcattc atattcaata aattgcaacg tcatgtaggc
    17641 cggatagggc gtttacgccg catccggcat ctgcgccatc ttcagtatct gacacaaaac
    17701 tatcgtttta acctttccgc tctacggaaa tcatggtgac aaaaggatag cgttgccacg
    17761 ggattgcccc gcctttcata tacatatgtg aaatcgtgcc atcaagataa agcagctgct
    17821 caacgttcag tttcgctttg gcataacagg caaaatcata aaaatttgtc gcctgctggc
    17881 tcaacaaaaa cacggcattc ccatgtttat taatcccaac accgttacga attttacgtg
    17941 aggcgacgtt gggatgaata cgcggattaa ttacactgtt ttccatcaac attggccctg
    18001 actgcaccgc aaactgaatc tctttactgg ttttgaaggc atccagacga acgatgccga
    18061 ctttatctcc cgcgacataa aacacgccgc caggacggat aaagaaattc ccttcacctg
    18121 aagcgagatt taacgccacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat
    18181 agctttcatc atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattcatat
    18241 ccgccagcag agcatgtaaa gttccccacg cttcgccatt ggctttttgc cagtacattt
    18301 tcacccgctc tgtttgagga ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga
    18361 gtgcgcaatc atcagcggca acagcaaaca agggaagtaa ggtgagggcg agaaaaatcc
    18421 gtttgagatt caaggtgatc attcctttac caatgagtag ccgatgcgcc attataggtc
    18481 ctggatgttg gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt
    18541 tcaactattg ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca
    18601 tgcgcgcacg atcactctaa gaggacattc gccttggaca cacccagtag atactggctc
    18661 actatcctgt catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg
    18721 gttgtcagcg acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac
    18781 ccaatttgtt tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt
    18841 catgctgagc gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc
    18901 acaacccctt gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact
    18961 gcgcgtacaa tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga
    19021 agcatcggca cgtttctttg aagacgatga cggcctgcat attcactcct tcttcttctt
    19081 tgaagatgcg gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg
    19141 tctgtttact ctgcgtgagc gtgaactgcc tgcattccgt ctgtatcgta tgcgcgcccg
    19201 tagccagtca atggtggacg gtaacgccta cgagctgctg ttggatctgt tcgaaaccaa
    19261 aatcgaacag ctggcagatg aaattgaaaa tatctacagc gatctggagc agttgagccg
    19321 ggtaattatg gaagggcatc agggcgatga gtacgatgag gcgctctcca cgctggcgga
    19381 actggaagat atcggctgga aagttcgcct gtgtctgatg gatacccagc gtgcgctcaa
    19441 cttcctggta cgcaaggcgc gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct
    19501 gcgagatatc gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct
    19561 gatgcaggcg gcgatgggct ttatcaacat cgagcagaac cgcatcatca aaatcttctc
    19621 ggtggtatcc gtggtattcc tgccgccgac gctcgttgct tccagctatg ggatgaactt
    19681 tgagtttatg ccagaactga agtggagttt cggctaccca ggcgcgatta tctttatgat
    19741 cctcgcgggc ctggca
//
LOCUS       DAFAVF010000073        19310 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFAVF010000073 DAFAVF010000000
VERSION     DAFAVF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.073"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            44..1405
                     /locus_tag="LGK42_002599"
     CDS             44..1405
                     /locus_tag="LGK42_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658835.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(1454..3118)
                     /gene="tsr"
                     /locus_tag="LGK42_002600"
     CDS             complement(1454..3118)
                     /gene="tsr"
                     /locus_tag="LGK42_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBK2658836.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(3237..3683)
                     /gene="hpaR"
                     /locus_tag="LGK42_002601"
     CDS             complement(3237..3683)
                     /gene="hpaR"
                     /locus_tag="LGK42_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBK2658837.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3955..5244
                     /locus_tag="LGK42_002602"
     CDS             3955..5244
                     /locus_tag="LGK42_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBK2658838.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            5241..6707
                     /gene="hpaE"
                     /locus_tag="LGK42_002603"
     CDS             5241..6707
                     /gene="hpaE"
                     /locus_tag="LGK42_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2658839.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6709..7560
                     /gene="hpaD"
                     /locus_tag="LGK42_002604"
     CDS             6709..7560
                     /gene="hpaD"
                     /locus_tag="LGK42_002604"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBK2658840.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7570..7950
                     /locus_tag="LGK42_002605"
     CDS             7570..7950
                     /locus_tag="LGK42_002605"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBK2658841.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            8061..8864
                     /gene="hpaH"
                     /locus_tag="LGK42_002606"
     CDS             8061..8864
                     /gene="hpaH"
                     /locus_tag="LGK42_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBK2658842.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8875..9663
                     /gene="hpaI"
                     /locus_tag="LGK42_002607"
     CDS             8875..9663
                     /gene="hpaI"
                     /locus_tag="LGK42_002607"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBK2658843.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9838..11214
                     /gene="hpaX"
                     /locus_tag="LGK42_002608"
     CDS             9838..11214
                     /gene="hpaX"
                     /locus_tag="LGK42_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBK2658844.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            11224..12114
                     /gene="hpaA"
                     /locus_tag="LGK42_002609"
     CDS             11224..12114
                     /gene="hpaA"
                     /locus_tag="LGK42_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBK2658845.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            12365..13927
                     /gene="hpaB"
                     /locus_tag="LGK42_002610"
     CDS             12365..13927
                     /gene="hpaB"
                     /locus_tag="LGK42_002610"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBK2658846.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13945..14457
                     /locus_tag="LGK42_002611"
     CDS             13945..14457
                     /locus_tag="LGK42_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBK2658847.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14850..17000
                     /gene="btsT"
                     /locus_tag="LGK42_002612"
     CDS             14850..17000
                     /gene="btsT"
                     /locus_tag="LGK42_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBK2658848.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            17050..17253
                     /locus_tag="LGK42_002613"
     CDS             17050..17253
                     /locus_tag="LGK42_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBK2658849.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            17264..18220
                     /gene="yjiA"
                     /locus_tag="LGK42_002614"
     CDS             17264..18220
                     /gene="yjiA"
                     /locus_tag="LGK42_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBK2658850.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(18254..18556)
                     /locus_tag="LGK42_002615"
     CDS             complement(18254..18556)
                     /locus_tag="LGK42_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HBK2658851.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(18543..18830)
                     /locus_tag="LGK42_002616"
     CDS             complement(18543..18830)
                     /locus_tag="LGK42_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HBK2658852.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            complement(19029..19310)
                     /locus_tag="LGK42_002617"
                     /pseudo
     CDS             complement(19029..19310)
                     /locus_tag="LGK42_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         4424 a         5278 c         5110 g         4498 t
ORIGIN      
        1 acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat
       61 caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc
      121 agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt
      181 attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt
      241 aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt
      301 ttcactcgct tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc
      361 acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcaa tgtctggcat
      421 ggtgcacaac tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc
      481 gatgaaccca tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg
      541 cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct
      601 ggcggcgatg atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg
      661 gatttttctc gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac
      721 tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag
      781 ttttgccgaa tggcgcagcc tgttccgtaa ccgtacaatg tggggaatga tgctcggatt
      841 cagtggtatc aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac
      901 agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg
      961 ggctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc
     1021 tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac
     1081 gctggtcgta ccacaagcga caacatccat gacggcggtt ctgctgattg gcatggcact
     1141 gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc
     1201 tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt
     1261 tgcgccgatc attactggtt ttattgttga taccacccac tcattccgtc tggcactaat
     1321 catctgcggt tgcgtcaccg cagcgggggc actggcgtac atcttcctgg ttcgtcagcc
     1381 gatcaacgac ccacgtaaag attaatctcc ttatgcccga taacattttg cttatcgggc
     1441 attttcatgg cgattaaaat gtttcccagt tctcctcgct atctgccacg gccattttac
     1501 gcggcgcagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgct
     1561 gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccagcg
     1621 cggcggcggc agcggcagat tcttccacca gcgcggcgtt ctgttgagtt acccggtcca
     1681 tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag
     1741 caatttcgcc cataatgtcc gtcacgcggg tcacggcgct gacaatctcc gccattgttt
     1801 ccccggcgct ttcgaccagc gtagagccaa catccacttt ccccaccgag tcttcaataa
     1861 ggcttttaat ttcacgagcc gcctgagcgc tacgctgggc cagattacgc acttctcccg
     1921 caaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc tgcttcaacc gccgcgttca
     1981 aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt
     2041 tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc actttgccgc
     2101 cccgttgcgc cgtttcagaa gcacttaatg ccagatggct ggcctggcgc gcattctcgg
     2161 cgttctgttt caccgttgcg gtcagttgct ccatgctggc tgccgtctct tccagcgaag
     2221 cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt tcgctggcac
     2281 cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct
     2341 gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg
     2401 gtttcaccag atcgccgcct gcaatatgac ggatgctgtc aatcaggcga ttcattggcg
     2461 ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt acgacgatca
     2521 tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg ctgacggcga
     2581 tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtctt
     2641 gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct aacagttgga
     2701 tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg
     2761 cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt ttttccgcct
     2821 gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg ctaccaatat
     2881 tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca
     2941 acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga
     3001 aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa
     3061 ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat cttgatacgt tttaacatgg
     3121 tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa ctttatgaaa
     3181 aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt atcagatact aaaaagttat
     3241 tcattatcgc ccgggatggc ttcttgtcgg gaattgccca gagcaataaa ttcctctaac
     3301 agatgggtta actgttgcat tttctcggca gtaaactgag cttcaatctg ccgataggct
     3361 tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt tagcgagata
     3421 tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc gcgttccatc
     3481 cgcgtaagga tcccggtcag gcttgggcgc aaaatgcatg cgcgatacgc cagatcgtga
     3541 aaatccattg atgggctttc cgccagaata cgcacgatgc gccactgttg ctcagtcaaa
     3601 ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc ctggagcaac
     3661 gcaatggtta gagagtcgtg cattatcttt cccctgaaaa attcaaaatc attaatatag
     3721 aaacagttaa tcatcacgtt accgggactg cgctgcccct gcaattcttc attttatcaa
     3781 aattgagcat gttttgttaa cttgctgata tttaatattt tattatcttt aaatgtaaat
     3841 agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc aatcgggaat
     3901 aaaagtaatc attaacatat taatgattaa aatgatcgaa tccgaggagt ggtaatgaaa
     3961 ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg
     4021 ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat taaaccgcgc
     4081 aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa ggtactgagc
     4141 ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga aagtacgtga agaagatgcg
     4201 gcagagtaca tcgccggata tgcgctggct aacgacgtca gcctgccgga agagagcttt
     4261 taccgcccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg cgaaaccgtg
     4321 gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg tcctgccgat
     4381 cactggaata ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa
     4441 tttgccacgc tgaatccagg cgacgccatt ctgctcggca cgccacaggc gcgcgtggaa
     4501 atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg
     4561 gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgcagcc acacccgcac
     4621 ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact ggaatttaag
     4681 ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg cgataaccag
     4741 acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct ggtggtagtt
     4801 attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt cgcgggctac
     4861 accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg ccctaacctg
     4921 cgggtcaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg
     4981 atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt acgccagcaa
     5041 ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt aagcgaattt
     5101 atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt atctgacgtg
     5161 gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt
     5221 gtgagtgagg atacagcgaa atgaaaaaag taaatcattg gatcaacggc aaaaatgtcg
     5281 caggtaacga ctacttccag accaccaatc cggcaacggg tgaagtgctg gcggatgtgg
     5341 cctctggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag gcgttcccga
     5401 aatgggccaa tctgccgatg aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga
     5461 tcgaccagaa cgtgcctgag atcgccgcga tggaaaccgc cgacacgggc ctgccaatcc
     5521 atcagaccaa aaatgtgttg atcccacgcg cttcccacaa ctttgaattt ttcgcggaag
     5581 tctgccagca gatgaacggc aagacttatc cggtcgacga caagatgctc aactacacgc
     5641 tggtgcagcc ggtaggcgtt tgtgcactgg tgtcaccgtg gaacgtgccg tttatgaccg
     5701 ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg
     5761 aactctcccc gctgaccgcc gaccgcctgg gtgagctggc gctggaagcc ggtattccgg
     5821 caggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggggatgcg ctggttcgtc
     5881 atcatgacgt acgtgccgtg tcgttcaccg gcggtacggc caccgggcgc aacatcatga
     5941 aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg taaatcgccg gtgctgattt
     6001 ttgaagatgc cgatattgaa cgcgcgctgg acgccgccct gttcaccatc ttctcgatca
     6061 acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc tacccggaat
     6121 tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga
     6181 atacccaggt tggcgcgctt attagccagc agcactggga aaaagtctcc ggctatatcc
     6241 gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc
     6301 tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca gacgttgata
     6361 accgtatgcg agtcgcccag gaagagattt tcgggccggt cgcctgcctg ctgccgttta
     6421 aagacgaagc cgaaggctta cgtctggcaa acgacgtgga gtacggcctc gcgtcgtaca
     6481 tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa gctggcatgg
     6541 tgttcgtcaa cacccagaac gtgcgtgacc tgcgccagcc atttggcggc gtaaaagcct
     6601 ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg
     6661 tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat gggtaagtta
     6721 gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact gccagggaaa
     6781 aaccacggtt gccgccaggg cgcgatcgac gggcataaag agatcagcaa gcgttgccgg
     6841 gaaatgggcg tcgataccat tatcgttttc gatacccact ggctggtcaa cagtgcttat
     6901 cacatcaact gtgcagacca ttttgaaggc gtctacacca gcaacgagct gccgcatttt
     6961 atccgcgata tgacctacaa ctacgagggc aacccggagt tggggcagct tattgccgat
     7021 gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa
     7081 tacggcacgc tggtgccaat gcgctacatg aatgaagaca agcacttcaa agtggtttcc
     7141 atttcagctt tctgcactgt tcacgatttt gccgacagcc gcaagctggg cgaggcgatt
     7201 ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg
     7261 caccgcttta ttgacgatca gcgagcggaa gaagggatga acagctacac ccgcgagttc
     7321 gaccgccaga tggacgagcg tgtggtgaag ctgtggcgcg aaggccagtt caaagagttc
     7381 tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat gcacgacacg
     7441 gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga gtttatcacc
     7501 gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct tcccgcgtaa
     7561 ggacgtttta tgccgcactt tatcgttgaa tgcagtgaca acatccgcga agaagccgac
     7621 ctgccggggt tgttcgccaa agtgaatccg acgctggcag ccacgggtat ttttccgctg
     7681 gcgggtattc gcagccgcgt gcattgggtc gatacctggc agatggccga cgggcagcgt
     7741 gattatgcct tcgtgcatat gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgt
     7801 cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc acttcgccgc cctgatggag
     7861 agccgcctgc tggcgttgtc gtttgagatt gaagagctgc atccgacgct gaattttaaa
     7921 caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt gcccggtggc gctgcgctta
     7981 ccgggcctac aaaaccccaa accgtacacc gtaggccgga taaggcgcag ccgcatccgg
     8041 caatgccaca ggatatcgct atgttcgata aacataccca caccctgatc gcccagcgtc
     8101 tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcgctggat tacccggaga
     8161 tcaccatcga agacgcttac gcggtgcagc gtgaatgggt tcgcctgaaa atagccgaag
     8221 gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag gccagctcac
     8281 aaatcagcga gccggattac ggtgccctgc tggatgacat gttcttccac gatggcagcg
     8341 atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg
     8401 caaaaccgct gcgtggacca aactgcacgc ttttcgacgt ctacaacgcc acggactacg
     8461 tgatcccggc gctggagctg atcgacgctc gctgccacaa catcgatccg gaaacccagc
     8521 gcccgcgcaa agtgttcgac accatttctg ataacgccgc caacgccggg gtgatcctcg
     8581 gtggtcgtcc cattaagccc gatgagttgg atctgcgttg gatctccgcc ctgatgtatc
     8641 gcaatggcgt gattgaagaa accggcgtcg ccgctggtgt gctaaatcat ccggcaaacg
     8701 gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa gccgggcaaa
     8761 tcattctcgg cggttcgttc acccgcccgg ttccggcgcg taagggcgac accttccacg
     8821 tcgattacgg caacatgggc tccattagct gccgctttgt ttaaggagag aacgatggaa
     8881 aacagtttta aagcggcgct gaaagcaggc cgcccgcaga tcggattatg gctggggctg
     8941 agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt gttgatcgac
     9001 ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc
     9061 taccccagcc agccggtagt acgtccgtcg tggaacgatc cggtgcaaat caaacaactg
     9121 ctggacgtcg gcacacaaac cttgctggtg ccgatggtac aaaacgctga cgaagcccgt
     9181 gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg cagtgcgctg
     9241 gcccgcgcct cgcgctggaa tcgtattcct gattacctgc aaaaagccaa cgatcaaatg
     9301 tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca gattctggac
     9361 gtggaaggcg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga tatgggttat
     9421 gccggtaatc cgcagcaccc ggaagtacag gccgccattg agcaggcgat cgtgcagatc
     9481 cgtgaatcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc aaaacgctat
     9541 ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc
     9601 gctgaagcgc tggcagcacg gtttggtgcg caggccaccg ccgtgaagcc cggcgtgtat
     9661 taatgccgga tgcgctgcac ttatccggcc tacactcgca ccaaaccgta ggccggataa
     9721 ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg cggaccggat aaggcgtttg
     9781 tgcgcatccg ccataaacct tgtcgtaccc tacaaaaatc ccattagagg aagaaaaatg
     9841 agcgacacct cacctgccat accggagagt atcgatccgg cgaatcagca taaagcgctg
     9901 actgccggac aacaggcggt tattaagaag ctatttcgcc gcctgatcgt ctttctgttc
     9961 gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg gctttgccgg actcacgatg
    10021 ggacgcgacc tcggtctgag cgccaccatg tttggcctcg ctaccaccct gttctatgcc
    10081 gcttatgtca tcttcggcat tcccagcaac attatgctga gtattgtcgg tgcgcggcgc
    10141 tggatcgcca ccatcatggt gctctggggc atcgcctcta ctgccaccat gtttgccact
    10201 ggccccacca gcttgtacgt actgcgtata ctggttggca ttaccgaagc cggctttctg
    10261 cctggtattc tgctgtattt aaccttctgg tttccggcct atttccgcgc ccgtgccaac
    10321 gccttgttta tggtagcaat gccggtaacg acagcgttgg gctcgatcgt ttccggctac
    10381 attttgtcgc tggatggcgt catggcatta aaaggctggc agtggctgtt tttgctggaa
    10441 ggcttcccgt cggtattact cggcgtcatg gtgtggttct ggcttgatga ctcaccggac
    10501 aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc aagagatgat ggataacgat
    10561 cgtctgacgc tggttcagcc agagggagcc atcagccacc acgccatgca acaacgcagt
    10621 atgtggcggg agatcttcac tccggtggtg atgatgtata ccctagcgta tttctgcctg
    10681 accaacacac ttagtgcgat cagcatctgg acaccgcaga tcctgcaaag ttttaatcag
    10741 ggcagcagta atatcaccat cggcctgctg gccgccgtac cgcagatttg taccattctc
    10801 gggatggtct actggagccg tcactcagat cgccgccagg aacgaaggca tcacaccgcc
    10861 cttccttatt tgttcgctgc cgcaggttgg ttactggctt cggcaactga tcacaacatg
    10921 atccagatgc tggggatcat tatggcttcg accggatcat tcagcgcaat ggcgattttc
    10981 tggacaacac cggatcagtc catcagcctg cgggcacgag cgatcggtat tgcggtgatc
    11041 aacgccactg gcaacattgg ttcagcgtta agtccgttta tgatcggctg gttgaaagat
    11101 ttgaccggca gctttaacag tggattgtgg tttgttgccg cgctgctggt gattggtgcg
    11161 gggattatct gggcgattcc aatgcagtcc tcccgtccgc gagcgacccc gtaaggaacg
    11221 acgatgtgtg accgtcagat tgccaatatt gatatcagca aagagtacga tgaaagcctg
    11281 ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg cggccttttt tggccgccat
    11341 atgctgccac atcgccacga acagtacttt cagatgcatt tcctcaatag cggacagatt
    11401 gagctacagc ttgacgatca tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg
    11461 ccgtcagtac ctcatgcgtt tattacggag tctgatgctg acggtcatgt attgacggta
    11521 cgggaagatc tgatctggcc cctgctggaa gttctttatc cgggcactcg ggaaaccttc
    11581 ggcctgccgg ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa
    11641 cactattggc aactgataga gcgggaatcg gtagaacaac tgcctggacg ggaacacacc
    11701 ctgacgttac tggcacaggc agtgttcacc ctactgctgc gtaacgcaaa actcgacgac
    11761 catgccgcca gcggaatgcg cggagaatta aaactgttcc agcgttttca tatgcttatt
    11821 gaaagccatt ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc
    11881 gaatcacgcc tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt
    11941 ttcgacaggc agctacgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac
    12001 aatattgcct ggcaactcgg ttttaaggat ccggcttatt ttgcgcgctt ttttaatcgc
    12061 ttagtcggtt gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaattcc
    12121 cttagcctta acgggaaacc aggcaccacc tgctattccc ctttcttcgc ctggagcaag
    12181 gggatttagt aacgttaaaa atcgctaaaa gcgacctcga tcacaaaacc gtacccgacc
    12241 tgaaaagtac cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa
    12301 ttgaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt
    12361 cgacatgaaa ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta
    12421 tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt
    12481 caccactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct
    12541 acacaaaccg gagatgcagg actctctgtg ctggaacacc gacaccggta gcggcggcta
    12601 tacccataaa ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgcgacgc
    12661 catcgctgag tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc
    12721 cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc
    12781 ccgtaactgg tatacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa
    12841 cccaccgatc gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga
    12901 aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca ccaactcggc
    12961 gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg aaaacccgga
    13021 cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtgaaattaa tctcccgcgc
    13081 ctcttatgag atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg
    13141 ctttgatgag aatgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt
    13201 actgatctac cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg
    13261 tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact
    13321 gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct
    13381 cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga
    13441 agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg
    13501 cgtactggca ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag
    13561 tggcctgatc tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta
    13621 tctggcgaag tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct
    13681 caaactgatg tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat
    13741 caactactcc ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc
    13801 cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa
    13861 cggctggact gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct
    13921 gaaataacgc agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac
    13981 gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa
    14041 tgcgggatta cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg
    14101 tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc
    14161 aacgtcctca accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg
    14221 gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg
    14281 ctgaaaggtt cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca
    14341 catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt
    14401 atctacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct
    14461 ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca
    14521 cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa
    14581 ataaccactc agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag
    14641 gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga
    14701 ttaacaaatt actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg
    14761 gagcttacaa cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa
    14821 agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac
    14881 cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg
    14941 gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt
    15001 atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga
    15061 cgcctgcggt tattaacaac gacggtctga actacgttcc gactaaccgt tacgtgttgt
    15121 ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg
    15181 cacagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagtg ctggccggtg
    15241 cggtacagga ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg
    15301 agatgatcaa agaagagatg gggccagtac cggggactat cgcgctgttt ggctgtttct
    15361 taatcatgat catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa
    15421 gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct
    15481 acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc
    15541 tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac
    15601 tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat
    15661 tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg
    15721 gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg
    15781 cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct
    15841 tgttcattac catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta
    15901 cgacgccgaa actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc
    15961 tgatggagtc cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc
    16021 tttacttcgc gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg
    16081 aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg
    16141 cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg
    16201 cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgccg acgctggcgg
    16261 taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc
    16321 acttcggtat tctgttcgaa gcactgttca tcctgaccgc gctggatgcg ggtacccgtt
    16381 ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg
    16441 actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc
    16501 tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta
    16561 tttccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga
    16621 tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca
    16681 ccacctgggc gctgggcctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct
    16741 acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga
    16801 ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcaggtctg agtattctgt
    16861 tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta
    16921 acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga
    16981 agatctcttc gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca
    17041 gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag
    17101 atgttgattg gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc
    17161 gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaacgc acgctacggc
    17221 ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt
    17281 taccctactc accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa
    17341 cgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga
    17401 tgatcaactg attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg
    17461 ctgttcgcgc tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa
    17521 gggcaatatt cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc
    17581 gattattcag acctttttct cccatgagat tttatgccag cgctacctgc tggacggcgt
    17641 gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca
    17701 gtcccaggtt ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc
    17761 agagaaactg cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac
    17821 ccacggcgat atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa
    17881 cgtcgtcagc accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc
    17941 gattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa
    18001 cctgctgctg gagtcggcgg ataaactact gcgttacaaa gggatgctat ggattgacgg
    18061 cgaacctaac cgcctgctat tccagggcgt ccaacgcctc tacagcgccg actgggaccg
    18121 cccgtggggc gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga
    18181 ggaagagatt cgggcagcgt ttgcggggtt gaggaagtaa ttttggatga tggccgccat
    18241 gtggcggcct gaattatttt ttgttctgat cgcgcagcat cgcttcacgt aaaattgcgt
    18301 tcaggcgagt ttgatagcct ttacctggtc gcttcaacca ttccatcaca tcagcatcaa
    18361 tacgcaccga agcctgagtt ttcaatggcc gataaaactt accgcgttcg gcgttagacc
    18421 attgctcatc gctggaaggt gggatatcac tgtaatcaat atcttcgtcg gatttattcg
    18481 ccagcgcctt cagttcggcc tcatgctgca catcgagtgt agaggaggtc cctcgtttat
    18541 gtttaaccat gctcatagcg attcctctct ttacgatctg cttttcgtgc actgatgatt
    18601 cgaataattt catttccgct ttcgaaacgg atggtatgcg caaccagaat aaccacgatg
    18661 ccgtaaacca ggccgattgt ctgccagcga tactcaccgt tttctatacg ttcttgctgt
    18721 gacaaatgtt gtggatcatc aaacaataac accgcatctt caaagcggat gccatgtttc
    18781 acccgattac ttttggcctt gttttcatcc cattcaaact ccattggcat aattgccatc
    18841 cctaattgta tatacaaaaa tgtatctaca attattctgc aattatagcg ttgatgttgg
    18901 aaatagcatc acgtttcgaa taatcaacca tgaaattgac aatgagtaag ttggacttga
    18961 ttcatccaat agaatttctc gcctgacatg gcacactaaa cacctcgctg tcgggtaatg
    19021 actccaactt actgatagta ttttatgttc agataatgcc cgatgacctt gtcatgcagc
    19081 tccaccgatt ttgagaacga cagtgacttc cgtcccagcc ttgccagatg ttgtctcaga
    19141 ttcagattat gtcgctcaat gcgctgagtg taacgcttgc tgataacgtg cagctttccc
    19201 ttcaggcgtg attcatacag cggccagcca tccgtcatct ataccacgac ctcaaaggcc
    19261 gacagcaggc tcagaagacg ttccagtgtg gccagagtgc gttcaccgaa
//
LOCUS       DAFAVF010000074        19261 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFAVF010000074 DAFAVF010000000
VERSION     DAFAVF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.074"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            242..808
                     /gene="yqaB"
                     /locus_tag="LGK42_002618"
     CDS             242..808
                     /gene="yqaB"
                     /locus_tag="LGK42_002618"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBK2658853.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            805..1233
                     /locus_tag="LGK42_002619"
     CDS             805..1233
                     /locus_tag="LGK42_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2658854.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1306..2862
                     /gene="gshA"
                     /locus_tag="LGK42_002620"
     CDS             1306..2862
                     /gene="gshA"
                     /locus_tag="LGK42_002620"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBK2658855.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3012..3527
                     /gene="luxS"
                     /locus_tag="LGK42_002621"
     CDS             3012..3527
                     /gene="luxS"
                     /locus_tag="LGK42_002621"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK2658856.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3591..5129)
                     /gene="emrB"
                     /locus_tag="LGK42_002622"
     CDS             complement(3591..5129)
                     /gene="emrB"
                     /locus_tag="LGK42_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBK2658857.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5146..6318)
                     /gene="emrA"
                     /locus_tag="LGK42_002623"
     CDS             complement(5146..6318)
                     /gene="emrA"
                     /locus_tag="LGK42_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBK2658858.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6445..6975)
                     /gene="emrR"
                     /locus_tag="LGK42_002624"
     CDS             complement(6445..6975)
                     /gene="emrR"
                     /locus_tag="LGK42_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBK2658859.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7066..7401)
                     /gene="ygaH"
                     /locus_tag="LGK42_002625"
     CDS             complement(7066..7401)
                     /gene="ygaH"
                     /locus_tag="LGK42_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBK2658860.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7391..8128)
                     /gene="ygaZ"
                     /locus_tag="LGK42_002626"
     CDS             complement(7391..8128)
                     /gene="ygaZ"
                     /locus_tag="LGK42_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBK2658861.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8252..9436)
                     /locus_tag="LGK42_002627"
     CDS             complement(8252..9436)
                     /locus_tag="LGK42_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2658862.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASLAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9727..10719)
                     /gene="proX"
                     /locus_tag="LGK42_002628"
     CDS             complement(9727..10719)
                     /gene="proX"
                     /locus_tag="LGK42_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBK2658863.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10776..11840)
                     /gene="proW"
                     /locus_tag="LGK42_002629"
     CDS             complement(10776..11840)
                     /gene="proW"
                     /locus_tag="LGK42_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBK2658864.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11833..13035)
                     /gene="proV"
                     /locus_tag="LGK42_002630"
     CDS             complement(11833..13035)
                     /gene="proV"
                     /locus_tag="LGK42_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBK2658865.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13390..14349)
                     /gene="nrdF"
                     /locus_tag="LGK42_002631"
     CDS             complement(13390..14349)
                     /gene="nrdF"
                     /locus_tag="LGK42_002631"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK2658866.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14359..16503)
                     /gene="nrdE"
                     /locus_tag="LGK42_002632"
     CDS             complement(14359..16503)
                     /gene="nrdE"
                     /locus_tag="LGK42_002632"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK2658867.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16476..16886)
                     /gene="nrdI"
                     /locus_tag="LGK42_002633"
     CDS             complement(16476..16886)
                     /gene="nrdI"
                     /locus_tag="LGK42_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK2658868.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16883..17128)
                     /gene="nrdH"
                     /locus_tag="LGK42_002634"
     CDS             complement(16883..17128)
                     /gene="nrdH"
                     /locus_tag="LGK42_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK2658869.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRSFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17376..17705)
                     /locus_tag="LGK42_002635"
     CDS             complement(17376..17705)
                     /locus_tag="LGK42_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBK2658870.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17857..18201
                     /locus_tag="LGK42_002636"
     CDS             17857..18201
                     /locus_tag="LGK42_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBK2658871.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18238..18687)
                     /gene="alaE"
                     /locus_tag="LGK42_002637"
     CDS             complement(18238..18687)
                     /gene="alaE"
                     /locus_tag="LGK42_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBK2658872.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4854 a         4846 c         4955 g         4606 t
ORIGIN      
        1 tagcggtatt aaccgcaggc accagggagg ataacgttgc gtcagcaacg gcccgaaggg
       61 cgaacgccag tgagtaatcc tcccggatgc accatcttct cccaaaaatt tccttcaaaa
      121 atatctcatt tctaaataac tactttatcc atttcctgaa ttttcttctc cttcgccatc
      181 agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat ttttcgactt gcgagaagac
      241 catgtacgag cgttatgcag gtttaatttt tgatatggat ggcacaatcc tggatacgga
      301 gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac tacggtcttc agtacgatgt
      361 tcaggcaatg attgcgctta atggatcgcc cacctggcgt attgctcagg caattattga
      421 gctgaatcag gccgatctcg acccgcatgc gttagcgcgt gaaaaaacag aagcagtaag
      481 aagtatgctg ctggatagtg tcgagccgct tcctcttgtt gaagtggtga aaagttggca
      541 tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc gccatcgctg aggcattgct
      601 ggcgcacctg ggattacgcc gttattttga cgccgtcgtc gctgctgatc acgtcaaaca
      661 ccataaaccc gcgccagaca catttttgtt gtgcgcgcag cgtatgggcg tgcaaccgac
      721 gcagtgtgtg gtctttgaag atgccgattt cggtattcag gcggcccgtg cagcaggcat
      781 ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat cgcttttctc gttgtttgcc
      841 agtagttttc tcagcgctac actattaccc ggcaactccg aagtcgtgct ggtggcaatg
      901 ttgctttccg ggatcagtta tccctgggtt ttagttttaa cagcaacaat gggtaatagc
      961 cttggagggt taactaacgt tatccttggg cgtttctttc cattgcgtaa aacatcgcgc
     1021 tggcaagaga aagctaccgg ctggctgaaa cgctatggtg cagtcacgct attattaagc
     1081 tggatgcccg tggttggcga tttactgtgt ctgttagcgg gatggatgcg catctcctgg
     1141 ggaccggtaa tctttttttt gtgccttggt aaagcgttac gctatgttgc agttgcagca
     1201 gcgaccgttc agggcatgat gtggtggcac taattgtagg cctgcacata tggtcaccat
     1261 tacagttatg ctaattaaaa cgattttgac aggcgggagg tcaatttgat cccggacgta
     1321 tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt taaaggggat acagcgtggg
     1381 ctggagcgcg aaactttgcg tgttaatgct gatggcacac tggcaacaac aggtcatcct
     1441 gaagcattag gttccgcact gacgcacaaa tggattacta ccgattttgc ggaagcattg
     1501 ctggaattca ttacaccagt ggatggtgat attgaacata tgctgacctt tatgcgcgat
     1561 ctgcatcgtt atacggcgcg caatatgggt gatgagcgga tgtggccgtt aagtatgcca
     1621 tgctacatcg cagaaggtca ggacatcgaa ctggcacagt acggcacttc taacaccgga
     1681 cgctttaaaa cgctgtatcg tgaagggctg aaaaatcgct acggcgcgct gatgcaaacc
     1741 atttccggcg tgcactacaa tttctctttg ccaatggcat tctggcaagc gaagtgcggt
     1801 gatatctcgg gcgctgatgc caaagagaaa atttctgcgg gctatttccg cgttatccgc
     1861 aattactatc gtttcggttg ggtcattcct tatctgtttg gtgcatctcc ggcgatttgt
     1921 tcttctttcc tgcaaggaaa accaacgtcg ctgccgtttg agaaaaccga gtgcggtatg
     1981 tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc tcggctatac caataaatcg
     2041 caaagcaatc ttggtattac cttcaacgat ctttacgagt acgtagcggg ccttaaacag
     2101 gcaatcaaaa cgccatcgga agagtacgcg aagattggta ttgagaaaga cggtaagagg
     2161 ctgcaaatca atagcaacgt gctgcagatt gaaaacgaac tgtatgcgcc gattcgtcca
     2221 aaacgcgtta cccgcagcgg cgagtcacct tctgatgcgc tgttacgtgg cggcattgaa
     2281 tatattgaag tgcgttcgct ggacatcaac ccgttctcgc cgattggtgt agatgaacag
     2341 caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc tggctgatgc accggaaatg
     2401 agcagtagcg aacttgcctg tacgcgcgtt aactggaacc gggtgatcct tgaaggtcgc
     2461 aaaccgggcc tgacgctggg tatcggctgc gaaaccgcgc agttcccgtt actgcaggtg
     2521 ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa cgctggatag cattaacggc
     2581 ggcgaagcgt atcagaaagt gtgtgatgaa ctggttgcct gcttcgataa tcccgatctg
     2641 actttctctg cccgtatctt aaggtctatg attgatactg gtattggcgg aacaggcaaa
     2701 gcgtttgcag aagcctaccg taatctgctg cgtgaagagc cgctggaaat tctgcgcgaa
     2761 gaggattttg tagccgagcg cgaggcatct gaacgccgtc agcaggaaat ggaaaccgct
     2821 gataccgaac cgtttgcggt gtggctggaa aaacacgcct gacagaaaag aaaaaggcca
     2881 ctcgtgagtg gccaaaattt catctctgaa ttcagggatg atgataacaa atgcgcgtct
     2941 ttcatatact cagactcgcc tgggaagaaa gagttcagaa aattttaaaa aaaattaccg
     3001 gaggtggcta aatgccgttg ttagatagct tcacagtcga tcatacccgg atggaagcgc
     3061 ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg cgacgcaatc accgtgttcg
     3121 atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga aagagggatc cataccctgg
     3181 agcacctgtt tgctggtttt atgcgtaacc atcttaacgg taatggtgta gagattatcg
     3241 atatctcgcc aatgggctgc cgcaccggtt tttatatgag tctgattggt acgccagatg
     3301 agcagcgtgt tgctgatgcc tggaaagcgg caatggaaga cgtgctgaaa gtgcaggatc
     3361 agaatcagat cccggaactg aacgtctacc agtgtggcac ttaccagatg cactcgttgc
     3421 aggaagcgca ggatattgcg cgtagcattc tggaacgtga cgtacgcatc aacagcaacg
     3481 aagaactggc actgccgaaa gagaagttgc aggaactgca catctagtca gtaaactatc
     3541 ttcacaatta attgaaaaaa gccagttcaa atgaactggc ttagttgtac ttagtgcgca
     3601 ccgcctccgc cgccacctgc gccaaatggc ggtttagcaa accacaccag ccccagcagt
     3661 acgaggaata tcccggctga catccagaag atctcattgg cggaaataat cagcccctga
     3721 ttggtgatct gctgggcaat ccagcctgat gcctgctgtt gcgtcatccc aagcccttcc
     3781 agttgactgt acatcgcctg ggcattcggg ttgaacgggt ttaccgactc agtcaactgc
     3841 gcatggtgca tcgactcgcg gttggtccac atggtcgtgg ttatcgacgt gccgatagac
     3901 cccgccagcg ttcgcgtaaa gttagagagg ctcgatgccg ccgccagtcg ttccggtggc
     3961 aaaccagaca gcgtaatggt ggtcagcggc ataaagaagc aggccaccgc aaacccctgg
     4021 ataaactgcg gccaggccga cgcgccaaaa tccatacctg gttcaaaggt ataggcacgc
     4081 cagtagaagc agacggcata cataataaag ctgaaggtta ccagccgccg catatccagt
     4141 ttatgcgcga agcggccgat aatcggcgac aggatcaccg gaataatccc taccggcgca
     4201 gaggccaaac ctgcccaggt cgccgtgtaa ccgtagacct cctgcaacaa ctgcggcagc
     4261 agaacaatag cgccgaagta gagcatatac gcgaggctga tacacaagca gccgatggtg
     4321 aagttgcgcg acttaaacaa cgacagatcg actatcgggt tatcgtcggt cagctcccag
     4381 acaatcagga agcagatagc caccaccgcc accacggtaa gaatgataat ttcctgtgat
     4441 gaaaaccagt ccagctcttt accgcggtcg agcataatct gcaggctgcc gataccaata
     4501 accagcagtg ccagccccac ggcatcaatc cgccgccgtt cggtgcgggt ttcacgtccg
     4561 cgcagagttt gcagtgtcat caacaccacc gccacgccaa tcggcacgtt gatgaagaat
     4621 atccagcccc agtggtaatt atcgctgata taaccgccga ggatcgggcc gcaaattggc
     4681 gcgacaatca ccgtcatcga ccacaacgcc agcgcgatcg agcgtttggc tggcgggtag
     4741 ttattcagca atagactttg cgaaagcggg atcaacggcc cggcgacaat cccctgaatc
     4801 acgcggaaga agatcagcat attcaggctg ctggagacac cacacgccca cgacgcaata
     4861 gcaaaggcga tggtggacca aaggaacagt ttcacttccc cgacgcgctt tgccagccag
     4921 ccggtaagcg ggatcgagat ggcattcgcc accccgaaag aagtgattac ccacgttccc
     4981 tggctgagcg atgagcccag attcccggcg atagtgggga tcgccacgtt agcaatggtg
     5041 gagtccagca cctgcatgaa tgtcgccagt gacagcgcaa tcgtcataat gaccagttgc
     5101 gcgccttcca gcggtttttg ctgttgcatc acacgcacct ctggattagc cagcgttagc
     5161 ttttacgata tcgtcgatca gtttattgac aggtgccagg ctgatttcac gcgcggtact
     5221 taccgcgacc ggtgttgaac gtactttatt tgccagtacc tgaccgtcac ggttagtggt
     5281 attgacgctc accagcgtgg acaaaccaat gcgcagcggg tattgctcca gctgtttctg
     5341 gtccagttcg atacgcacag gtagacgctg aacgactttg atccagttac cggtcgcatt
     5401 ttgcgccgga agcagtgaga atgcgctacc tgtgcccata tccagaccaa ccactttacc
     5461 ggtgtatttc acatcatcgc cgtaaatatc cgtggtgata gtgaccggct gaccgatacg
     5521 catattggca atctgcgtct ctttaaagtt ggcatccacc cacatattgg tggctggaac
     5581 gaccgccatc agcggcgtcg ttgggctaat ttgcgcccca ggctgtaccg cgcggcggga
     5641 gacataaccg gtcatcggac tgacaatacg ggtacgctcc agcgccagcc aggcgttacg
     5701 tacttcggtg gcagcctgtt gcacggcagg ctgatcttcc agtttagtcc ccagaatcat
     5761 cgcctgattg gcattgtatt gttgaatagc gacgtccagt tgcgcctggg cactggtgac
     5821 ggcgtcgcgg gcgtgttgca gctcttcgcg accaatcagg ttggcattgc ccagcggcac
     5881 acggcggttg tagtcgcttt gtgctttcgc gagggcgatt ttttgcacct caatattcgc
     5941 ctgcaactgt ttgctgttaa tcatcagctg gtgggtttgg cgaacgctgg aagccagtgc
     6001 agttttggct ttttcaaacg cctggcgagc atctgtcggg tcgagagtga ccagaacatc
     6061 gccttctttt acaaaatcgg tgttatcggc ccagactttc gtcacgctgc cagacacctg
     6121 agacataatt tgaatctgat tccctgccac gtatgcgtca tcggtttctt cgaaatgacg
     6181 cagtaccaaa aaccaataaa tccctatcgc tacggcaata attataaaga gcaaggtgag
     6241 aaggaggagc agacgcttac gtttgccgct cttctttacc ggttgctgcg gggtttgagt
     6301 ctccgcattt gcgctcatat tgttctccac gatcttctta actcatcggc tgagccgacc
     6361 tgcttgccaa aaaggccagc atgttgcgat gctggccggt cattttttct tttataaatc
     6421 tggatttttg agcgagatga cgcgttagct catcgcttcg agaaccacac cgtcttgttc
     6481 catctggtcg agacgggaga gcaatttgcg ggtgatttgc tcgagctgat ctttttctgt
     6541 tgtgctgagc gcggaccaga gttgatgcag gcagttatgc tgcggtggta aaacctcgcg
     6601 caaaaactcg tgaccttttt ccgttaattg cagatgcagg cagcggcgat cgttatcgct
     6661 ttcacgacgt tcgatccaac cgcgtttttc cagttcatcg gcaatacgcg tcgcgttggt
     6721 acgggatgat ccaagagcac aacttaattc agaaggctga atactgtggt tttcctgaga
     6781 ctccagcgta atcaacgcca taaacaacgt ctcgttaatc ccctgagcct tcagcatttt
     6841 attgcggttc tccagcagct tgctttgcat gtgcatgcaa agacgagtca gaaggatctc
     6901 ctgataagga aaatcttcgt ggcggctggc gcgaaatttt agcatttgtt caatgggcgt
     6961 aaacgaacta tccatttggg tatgacctca ttaatttcag ccgatatagt aacgacagtg
     7021 acaaataaag taaatgtatt gttttagaaa aatgattctt gtgggttata taatcgccat
     7081 cactttccag gcgagcccat aggccagcgc actaagcagt gttgggatga taatgctgcg
     7141 tgttttatag aaactggcac ccagtaccgc gaagccgacc agcgtgggca cgaaacggcg
     7201 tgtatcgtgc atcacttctg gtgcggtaga gacaaccagc agagcgcata tcgaggcgat
     7261 gccaatggtg tcgagcaaaa tacctaccgc gccacgtttg gttgggcggg cattacccac
     7321 acgcaggcgc agcggcaaat agcggaagca ataattcgcc acgccaacca gtaacccaag
     7381 cagcagaacc tcatagctca tcgggcgctc cttgccagaa tgcctggatt aacgcagtga
     7441 ggcagccaca gacaatgcct gccagaatgg cgacgggaat agaaaatagc gttacgcctg
     7501 caagggcacc aactaacgct gcggtaacgc aaagagattg tttgcgctgg aaagaggcga
     7561 gcaggaaact cataaagagt gccggaagca taaaacctaa tgcagcttca acggcgggat
     7621 aaccttgcag caagccgctg ccggagaatg cccctattac cgtaccaaat acccacgatg
     7681 accatgaact gaaggcaatg ccgatcatcc agttctcgct ccagcggcga ttattgcgta
     7741 ccagttttgc ggttgcggcg gcaaaaacct catccgtcag gccaaatgcc cacagggcgg
     7801 ttttcgattt ttgcagacgc tgaataatac ggctacgcag tgacgggcca tacaacacat
     7861 ggcgaacatc cattgccatg acggtcagtg cagcaaccca caaactactc ccggctgcca
     7921 gcatcgcggt aatgacgaac tggctcgcgc ctgcataaat gatgcaggag aaaaaaacgc
     7981 tttcgagagg agagaatccc agacgggtcg cattcagacc gaacgcaaag gccaccggaa
     8041 tataactaat aacaatcggt aaactgtctt tgcatccttc catgaaggtc gccgaaccag
     8101 gagcaggctg tggagtaggg ctttccatag agtgtacgct taacgattgt taatattata
     8161 aatagactga atgaatatct taaccttatc agactgatgg gcttcttaac acccttataa
     8221 gtgtaaagcc acgaaaacgg ttgctgattg cttaatttgc ggcttcctga cgatgaaaac
     8281 ctcgccacca gaccagtaag ttaaccaggg caatcgtcgc gccagccaga caaacgccag
     8341 cccaaccgcc atgttgccag gctgaggctg aaattagcga accggcggca ccgccaataa
     8401 agtagctggt catgtaacct gcggtcaggc gattacgcgc atcagggtgt attcgataaa
     8461 ttaccgtctg gttagtgata tgcacgccct gtacggtgag atccagcacc aggatgccga
     8521 taatcagcgc cagtacggaa gtgtgaccaa accagatcgc cagccatgaa agtaatagca
     8581 gcagcagacc gaaagttgtg gtgtggtgcg atttaccctt atcggcaaaa ccgcccgccg
     8641 gacgagcgcc caacgctccg gcagctcccg caagtccaaa cagaccaatg acaccatcgc
     8701 tgtagttaaa aggtggagcg gcaagcaaaa aggccattga ggtccagaga atgctgaagt
     8761 tggcaaaggt caggcagccc agcaatgcgc gggtgcgcag gatcttatcg ctgataaaca
     8821 tgctgaaaac ggaacctaac aactgtgggt agttgaggtg ggtttctgat ttcatttgtg
     8881 gcagaccacg ccataatgcc agcgccatca gtgccattaa caccgaggca acccaaaaga
     8941 cggtgcgcca gccgccgaga ttcgccagca atccggcaac ggtccgtgcg agtaagatcc
     9001 ccaacagcag accgctcata atagtgccaa ccactttgcc gcgtttgtcc ggtgaagcca
     9061 gcgtcgctgc cagcggaacc agaatttgtg ccacgactga gaataaaccg gttaatgccg
     9121 taccgaggat catcatcgcc agtgactgac tgctggcagt gattaacata ccgcccgctg
     9181 ccagtaaggt catcgagaca atcaggcggc ggcgttcaaa catatcaccg aggggaacaa
     9241 gaaacagtag acctgcggca tagcccaact gcgcggcggt aacaataaag cctgccaaac
     9301 tggcggaaag ggaaaagtta cgcgcgatgg tgtcgagcaa tggctgggcg taatagttac
     9361 tggctaccgc cagaccggtg gcgatagaca tcagcacgat cagcgccggg ctaagctcat
     9421 gattaggttt agtcattatt tttgttcatc aggtgatgat taagctggaa atcaataata
     9481 gcggatgttg atgaatatgg gatgaaagat agattgttga aatgtgcgat gtggtttgta
     9541 ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaacggc ggatgcggcg
     9601 aaaacgcctt atccgccata cgaatgagtg tattttgtag gcatgataag acgcgtcagc
     9661 gtcgcatcag gcattgtgta cagagcggcg gatgaggcaa aaacgcctta tccgcctgaa
     9721 taaaaattac ttctgcgctg ccagcgcctc attcacccag ccatcgaact gctgctggtg
     9781 ggctttgatc cagccatcaa cgtggccctg aatatcgcct tctgaggctt tgccgtcatg
     9841 cataatggcg ttctgggcgt taatatcggc cactggcaac tgcataatgg caaacagttt
     9901 ggctgccgcc ggatttttct cggtccaggc tttgttggca acgatatgca tggtgctgac
     9961 cgggaagccg taattcgcgc cattcggcag tttggtatcg gcatttttat cgcccggcag
    10021 tgcggagaac ggcacctgca accagaccac atctttccct ggcttcagct cattactcac
    10081 ccagtacggc gtccaggtgt aataaaacac cggtttgccc tctttgtagc gactgatggt
    10141 gtcggccatc atcgctgcgt agttcccctg attatgcgtc acggtgttgg tcagttcata
    10201 cgcggcaagc tggtggttga tggcaccttc gcagccccag ccagggttac aaccggttaa
    10261 atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg atcttcggat ctttcagttg
    10321 tgcgatgttg gtgattttat actggtcggc ggttttctta tcgatcaggt aaccctgtgc
    10381 cgcgccgtta acaaataccc cttcacgata aaatttctta tcgccaccgg cagcttcgta
    10441 catgttgtca tgcagcggcg tccagttcac ggcggtgaag gttgcatcgc cggaagcaag
    10501 cgaggtgtag ccaacgttgt aatctacttc gctgggttta ttgacggtat aacctaattt
    10561 ctccagcgca cgactgacca gcagtgtctg gaaggtttct tcggtgatgg tgctttgaac
    10621 tggattaact gtaatgcctt tgcccggcag gtcggcagca aaagtttgtg tagagataag
    10681 cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt gttattcctt tttttggcag
    10741 ggtgatatgc ccggcgttac cgggcaagtg cagagttact taatgaatgg gcgggtcagc
    10801 agaccgacag ggccagtggt gtaccagcga cggttgccgc gactgcgtga gtcgcgccca
    10861 acggcctgcg tcaggcggtc gaggataatg gcgaggatca caatcccgac gccgccaacg
    10921 gtggcaagcc ccatatccag acgaccgata ccgcgaagta ccatctgacc cagcccgccg
    10981 acggcaatca tcgaggcaat gaccaccata gaaagggcca gcatcagcgt ctggttaacg
    11041 cccgccataa tggtcggcat cgccagcggt aactgaactt tgaacagcat ctggcgcggg
    11101 ctggcaccga atgagcgcga ggcttcaatc agatccgccg gaacctggtt aatccccaga
    11161 atggtcagac ggataatcgg cggcagagca aagatgatcg tcaccaccac gcccggcacg
    11221 ttaccgatac caaatagcat gacgattggc accagataaa caaacgctgg cgtggtctgc
    11281 atggcatcaa gcagtggacg aataattttc gccgctcgcg gacttctcgc cagccagatc
    11341 cccaacggca aaccgatgac gatacagaac agcagggcgg ttaacaccag cgccagagta
    11401 accattgcct gcgaccaggc accgattgcg ccgatggcaa tcagcgaaac cagcgtcgcc
    11461 acgcccattc cgaccccgga aatctgccag gcgatgagag cgaaaacgat aatcgccacc
    11521 ggtgcgggca tacccagcag caactgctgg aaaccgttga ggatataatc aaccggaacg
    11581 cgcacgccct ggaagacggg acggaaatgg gtaacaaccc agtcgatccc ttcagtgacc
    11641 caactgtcga gcgggatcag cgttttatgg aacggatcga gaatattaaa atgctcgacg
    11701 tttggcgcag gcgtactggt cagccagtca gcaccaccgc cgtcagtcgg tgcagtcgcc
    11761 ggtgtacccc aggcgtctgc ggattgcgcg gcactgtccg ccgctggcgt ggtatcccac
    11821 ggattatttt gatcagccat tatttacccc ctcacgatct aaagcgcgca gcagcattcc
    11881 tttcgaaatg atgccgacat actgttggtc ctcgtcgacc acgggcaccg cacagggtgc
    11941 ctgtccgaca tgagagagca actcgctaag aggcgtttgt gcatcgactg ctaacggcgc
    12001 atcaatcagc gccgcatcaa gaccttgctg ctgcgttaac gcggttttaa gcgaatcgat
    12061 ggagactgcg ccgacaaact tattaccgcg ttcgataacg tagccatatt cgcgatcttc
    12121 atcctgcaat aatttcagtg ccgaacgtgg gccgaagcca ggggttttac gaattaagcc
    12181 attcggtgtc cggcgggcaa tatctttcgc actgaatacc tgactaatat caacgccacg
    12241 gaagaaggta cggacataat cattcgccgg attattgaga atttcatccg gtgtgccgac
    12301 ctgtaccact tcaccatttt gcataatggc aattcggtcg ccaatacgca tagcctcatc
    12361 gagatcgtgg gaaataaaga caatggtgcg ctgatgtttc gcctgtaatt ttaccagctc
    12421 atcctgcatc tcggtgcgaa ttaatggatc gagcgccgag aaggcttcgt ccattaataa
    12481 tatatccgga ttaatcgcta acgcgcgggc taatcccaca cgttgacgca tcccgccaga
    12541 gagttcatcc gggtagctgt gggcataatt ttccagcccg acctgacgca gtgcatcaag
    12601 ggctttttcc cggcgttctt cggcattaat tccggccaat tccataccga acgcagtatt
    12661 gtccagcacg gtcatatgcg gcattaaggc aaaggactgg aagaccatcg caatcttttt
    12721 tctgcgcacc tcacggagtt cggcatcgga tattttggca atatccacac catcaatcag
    12781 cacttgcccg cgggtgggtt caatcaggcg attgagaagg cgtaccattg tggatttacc
    12841 cgagccggat aatcccatga tgacaaatat ctcgccttct tcaatggcca gactggcgtc
    12901 ttttacgcca agcgatagcc cagttttttc cagaatttgt tctttcgaaa gtccttgttc
    12961 gatatatttg aacgctcgct gtggatgctc gccaaatatt ttataaagat ttttaatttc
    13021 taatttaatt gccatgcaat agaaagattc ctttatttgt ttatgtcgat atattaccca
    13081 atggaaatag tcactttttt ctaccctaac atactgagaa tctgaggcaa cccctgaggg
    13141 caaatgtggc atgaatattg atgcgagcaa atcgccggaa tttcccgtga tataagggct
    13201 gagagcaaat cgagaaaagt tgatattttt tgtcatagat agcgaaaaaa cgcctgaata
    13261 ttgtagtttc aggcgaataa tgcaacagag tatcagtgta gatcactaca gaatatttgc
    13321 gtgataaaaa gctatttggc gggaattatt tccctgctgc gggtagtgat atttttgaaa
    13381 ataacaccct cagaaattcc agtcttcatc ttctgtttca accgctttcc ccatcacata
    13441 agaggagcct gaaccggaaa agaaatcgtg attttcatcg gcattcggcg aaagcgcggc
    13501 gaggattgcc ggattcactt ccgccatttc tgcgggaaat aacggttcgt agcccagatt
    13561 catcaaagcc ttattggcgt tgtaacagag aaacgctttc acatcgtcag cccacggggt
    13621 ttcggcgtac agctcatcgg tgtattgcaa ctcgttgtcg tagagttcca gcaacaaatc
    13681 gaaggcgaaa ctcttcaact cttcacgttg tcccagagat atcttttcca tgtttttctg
    13741 atatttatag cctatgtagt aaccgtggac tgcttcatcg cggataatca gacggatcag
    13801 atctgcggta ttggtcagct ttccgcggct ggaaaaatac atcggcagcc agaaaccgga
    13861 ataaaacaaa aaagattcaa gaaacacact ggcgattttc tttttcagcg gatcatcacc
    13921 gcgataatgt tgctgaataa tctgagcttt tcgctgcaac ggtgcgtttt cttcactcca
    13981 ggcgtaggcg gcatcgacat ctttggtctg acacagcgtc gagaaaatcg aactgtaaga
    14041 gcgggcatga accgcttcca taaagctgat attcgataat accgcttctt catgaggcgt
    14101 gagtgcatcg ggcatcagcg aaggcgcgcc gataacattt tgcagcgtgt cgagcagcgt
    14161 caggccagta aaaacgcgca tcgtcagttg ttgttctacg acagttaatg tctgccaggc
    14221 aggaatatcg ttcgacagcg gcaccttttc tggtagccag aaattgctgg tcaggcgatt
    14281 ccacacctcc agatctttat cgtcagatat cttgttccag ttgatggcgc tgatacgtga
    14341 gagtttcata gatattcctt aaagtgcaca ggagacgcag ccttcaattt cagtgccttc
    14401 cagcgccatc tgacgcaggc gaatgtaata gagcgttttg atacctttgc gccaggcgta
    14461 aatctgcgct ttgttgatat cgcgagtggt ggcggtatcg gggaaaaaca gcgtcagcga
    14521 caaaccctga tcgacatggc gagtcgcttc cgcgtaggtg tcgatgatct tttctgcgcc
    14581 gatttcgtaa gcatcctgat acagcgccag attctcgtta gtcataaacg gggcagggta
    14641 gtaaacgcgt cctgttttgc cctctttgcg tatctctact ttcgccacaa tcggatgaat
    14701 actcgacgta gcatggttga tataagagat agaaccggtt ggcggcaccg cctgaagatt
    14761 ctggttataa atgccgtagc gcatcacgtc gtcgcgcagt tgcgcccaca tctcacgggt
    14821 aggtaacgta ataccgctac gggcaaacag ttcgccaact ttcgccgttt tcggctgcca
    14881 gttcccttgc agatattggc tgaaatattc accactggca tagcgtgact gtttgaaccc
    14941 ggcgaaggtt tcaccgcgtt cgcgtgccag caacatcgag gtacgcagtg cgtgccaggt
    15001 gatggtatag aaatagagat tggtgaaatc cagtgcttcc ggcgaaccat aagcgatgcc
    15061 ttctcgcgcc agataaccgt gtaaattcat ctgccccagc ccgatggcgt gtgaggcggc
    15121 atttccggct tcgatggacg gcacgctgcg gatatgactc atatctgaca ccgccgttaa
    15181 accgcgtacg gcagtctcta ccgtgcgggc aaagttgggg gaatccatgg tgtgggcaat
    15241 attcaacgaa cctaaattgc aggaaatatc atggcctgta cgggcatagt cgagattctc
    15301 gtcatactct gaggcgctgt taacctgcaa aatttctgag cagagattac tcatatttat
    15361 gcgcccggcg atagggttag cacggtttac cgtgtcttca tacatgatgt agggatagcc
    15421 ggactcaaac tggatttctg ccagtcgctg gaagaaatca cgggcgttga ggtatttttt
    15481 gcgaatgcgt tcatcggcaa ccagttcgtc atagtgttgg ctgatggcca catcggcaaa
    15541 cggcttgcca taaactcgct ctacgtcata aggcgaaaac agcgccatct gcgcattctc
    15601 ttttgccaga tggaaagtga tatccgggat caccacgccc agcgacagtg ttttaatgcg
    15661 gattttctcg tcggcatttt cccgtttcgt atcgagaaaa cgcagaatat cgggatgatg
    15721 ggcatgtaaa tagactgcac cagccccctg acgagcgccg agctggttgg cataggaaaa
    15781 tgcgtcttcc agcattttca tcaccggaat cacgccagaa gattgatttt caatacgttt
    15841 aattggcgcg cccgcttccc gcagattcga cagcaaaaat gccacgccgc cgccgcgttt
    15901 cgacagttgc agtgcggaat ttaccgcccg gccaatcgac tccatattgt cttcaatacg
    15961 cagcaaaaaa caggaaacca gttcgccgcg ctgctgctta ccacagttga ggaatgtcgg
    16021 tgttgcaggc tgaaagcgtc ctgacagcat ctcatcggtc agttgcaacg ccagtgtttc
    16081 atcgccttgt gccagcgtta gcgccaccat cgtgactcga tcggcaaaat cttccagata
    16141 acgcttaccg tcgaatgtct tcaacgtata gctggtgtaa aacttccatg ccccgaggaa
    16201 tgtctggaaa cgaaaaccgc tggtgtgtgc gtgggcaaac agcgtaatga caaagtcgcg
    16261 agagtagcga ttaagaacgc tttcatcata gtaaccttcg ttgaccagcc agttcaggcg
    16321 ctgctgctgg ctactgaagg tcacactgtt cggacgcaca tgcgtcgcca taaaggcgtc
    16381 aacggcctgg cggtctttat cgaactgaat gcgacctgcg ctatcgtaga ggttaagcat
    16441 cgcattcagc gcgtggtaat ccatcgtttc ctgcgtcagg cattctgcgg ttgtcgttgc
    16501 caaaattcgg ttactccttt acgaacgttt tcgatatcgc tttgcgtacc catgagttca
    16561 aaacggtaca gccacggcac gccgcatttc tgagcaatca catctccggc acggccatac
    16621 gcctcaccaa agttgcgatt acccgaagca ataacgccgc gaagcaacgc ccggttgtgc
    16681 tcgtcgttta aaaagcgaat tacctgtcgt ggcaccgcgc cagccgtacc gccgccgccg
    16741 taagagggca cgatcaggat gtaaggctcg tctacctgaa tccgttcccg ctcattgagc
    16801 gggatgcgca ccgcgggcag acctaaacgt tcgataaaac gctgcgtatt ttcggagctg
    16861 ctggagaagt agacgagctg gctcatgcac tggccgcgtg tggcgctgga tgcagacggt
    16921 taatcatgtc cggacggaaa ccagaccagc taagatcgcc agcaatcact accggcaact
    16981 gacgaaagcc ctgagcacgc aacgcttctg ccgcttcagg aacgcgatcg acattaatca
    17041 tttcaaaatc aaagctccgg ttttccatcg cccgtttggt ggcgtggcac tgaacgcaat
    17101 cgttacgagt gtaaatagta atgcgcatga ttcgtatttc cgtttaaaat gaagatacgg
    17161 cgcgatgata cgcgtcgggt tgtctcactg ttgatacaga gatactagat gtagttgaaa
    17221 aaagattcaa ccacacaata tatagcaaat agtggtcgaa attaccctgg atatgagcgt
    17281 gacggggtag gggaattttt gtgattcacc aggcaaaaag aaaccccgaa gacaggcttc
    17341 ggggtcaaag acgcgtattt attatcattt ttgcactacg atttgcgcat gcttaatagt
    17401 gcgccgataa aaatacctac cgcagctgct gtacccacgc tacaccaggg tctttcacga
    17461 acaaaagaat ccgcgcagcc aacggcatcg cgtgcggctt gctggacgcg agtacgacca
    17521 tgcattcgcg ccctggtttc tttcagcaat gcctgagctt tactgcgtgc ggcttcagct
    17581 tcccctttgg cgtcgctgcc ccaagacttt aatacagatt cgaggctgtc agctaattga
    17641 ttgacatcat tctggatatc ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta
    17701 aacatatgat cccccattag aattcgatgt aattttcagt gtagaccata attttcttaa
    17761 ctgaggttca tcgcggcttt atggactttt ctgaaagcgt tgtgaatgcg caatcaggta
    17821 aggtaggccc gcggttgata ataacgagga tcaaatatgt atttacgacc agacgaggtg
    17881 gcgcgcgtac ttgaaaaagt cggttttact gtcgatgtgg taacccagaa agcgtatggt
    17941 taccgccgtg gggaaaatta tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgct
    18001 ctggtaattc atccaacatt aaaagaacgc agttcaacac tggctgaacc cgcttccgat
    18061 attaaaactt gcgatcatta ccagcaattt ccgctctatt tagcaggcga gcgacacgag
    18121 cattacggta tcccgcatgg cttcagttcg cgtgttgcgc ttgaacgtta tttgaatggt
    18181 ttatttggcg aagccagtta aagacgcgac tggcgatgcc agtcgcgaaa agaagagtca
    18241 ggcttttacc tgctggtaac ggctgacttt aaacagtcgg cggcaataat cgaggaagta
    18301 gccataaacc gcccccatca acatcgaaac aacgatgttt gaactgaccg ccgccataat
    18361 ctgatgccag tctgcgccca ccactaacaa gatcgccaca tacaccggtg actggaacgt
    18421 cacataagcc aggatatcag ccagattttt tatccagccc gacgggctaa ctttgcgtgc
    18481 cgcgcgcata aacagatcgc ggtacatacc gtatggccat gcaattaaga tgttcaccgg
    18541 aatcgctacc aatctggaat aaaaagactg ttcgaagctc attccggaga ggaaaacttc
    18601 aatacacatg ttcacgacag aacagtaaac aaccatcgcg aacgtatctg caactgcatg
    18661 acgcaagcgt gactgcggtg agaacatgct aatgctcctt aataaaagcg taatgggatg
    18721 ttaatggaga tgaaagcagt ggatctcatt gccatctgca ttttcgcata gcgtatatcg
    18781 ctggctatga ctattcaatt agtgaaaaat ttttatttag tcagtttttg tagataaaat
    18841 attccgtaac ggctcgtttt ttgtacattg tggattttta catgccgata taatttattt
    18901 tcaattaaat caattagata tgaccttgcc tttaatttca ctaatgaaag tttgggggtg
    18961 aggtaacgct ataagcgtag agaattcggt gtatgtcgtg accgtgtcat ttacagcatt
    19021 ttttatgcca gaaaatgtac attaacagca gaaagcacca gtgaggggtg ttttctttta
    19081 ttctgttata ttgcattttt attcacttcg gttttgtcag gcttgcggaa ttagcgagca
    19141 gagagcgcca ctctgttcac caataatcta aattactcat caaaatggct aatatataat
    19201 cttgaaatta ttctgagagt ttcagaaaat aacagctgaa ataatctcgc gcaggactgt
    19261 a
//
LOCUS       DAFAVF010000075        19224 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFAVF010000075 DAFAVF010000000
VERSION     DAFAVF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.075"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..369
                     /locus_tag="LGK42_002638"
     CDS             <1..369
                     /locus_tag="LGK42_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658873.1"
                     /translation="AKHNRQDVLCCVSCGCPLTLQRGNDGQPPWFEHDQMTVAEKILL
                     RCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDEDYEGEKCCPRCGTGVYSRA
                     WGRQEVPSEDARADNPLQRL"
     gene            724..1104
                     /locus_tag="LGK42_002639"
     CDS             724..1104
                     /locus_tag="LGK42_002639"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBK2658874.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1101..1448
                     /gene="tnpB"
                     /locus_tag="LGK42_002640"
     CDS             1101..1448
                     /gene="tnpB"
                     /locus_tag="LGK42_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK2658875.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1498..2883
                     /locus_tag="LGK42_002641"
     CDS             1498..2883
                     /locus_tag="LGK42_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK2658876.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTRTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3122..4480)
                     /locus_tag="LGK42_002642"
     CDS             complement(3122..4480)
                     /locus_tag="LGK42_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBK2658877.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPYFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            4762..4893
                     /locus_tag="LGK42_002643"
                     /pseudo
     CDS             4762..4893
                     /locus_tag="LGK42_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5181..5702
                     /gene="fecI"
                     /locus_tag="LGK42_002644"
     CDS             5181..5702
                     /gene="fecI"
                     /locus_tag="LGK42_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBK2658878.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGASSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            5699..6652
                     /gene="fecR"
                     /locus_tag="LGK42_002645"
     CDS             5699..6652
                     /gene="fecR"
                     /locus_tag="LGK42_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBK2658879.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSSERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TSEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQLDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            6739..9063
                     /gene="fecA"
                     /locus_tag="LGK42_002646"
     CDS             6739..9063
                     /gene="fecA"
                     /locus_tag="LGK42_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBK2658880.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDANRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            9108..10010
                     /gene="fecB"
                     /locus_tag="LGK42_002647"
     CDS             9108..10010
                     /gene="fecB"
                     /locus_tag="LGK42_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBK2658881.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            10007..11005
                     /gene="fecC"
                     /locus_tag="LGK42_002648"
     CDS             10007..11005
                     /gene="fecC"
                     /locus_tag="LGK42_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBK2658882.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            11002..11958
                     /gene="fecD"
                     /locus_tag="LGK42_002649"
     CDS             11002..11958
                     /gene="fecD"
                     /locus_tag="LGK42_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBK2658883.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            11959..12726
                     /gene="fecE"
                     /locus_tag="LGK42_002650"
     CDS             11959..12726
                     /gene="fecE"
                     /locus_tag="LGK42_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBK2658884.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAGRRLTELSGGQRQRVFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(13284..13541)
                     /locus_tag="LGK42_002651"
     CDS             complement(13284..13541)
                     /locus_tag="LGK42_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658885.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(13622..13774)
                     /locus_tag="LGK42_002652"
                     /pseudo
     CDS             complement(13622..13774)
                     /locus_tag="LGK42_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13956..15041
                     /gene="dndB"
                     /locus_tag="LGK42_002653"
     CDS             13956..15041
                     /gene="dndB"
                     /locus_tag="LGK42_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HBK2658886.1"
                     /translation="MASVDADYCYSFPAIRGIQAGRPFYIATCPMRIIPKIFSFDEND
                     VPPELRAQRTLNKSRIPEMVRYLLENPKDYVFSALTASIAVDVQFDEFPGSNNLGTLC
                     VPMDAQILINDGQHRRKAIEDALDARPELGQDNIPVLFFVDEGLKRSQQMFADLNKYA
                     IRPSPSLASLYDHRDACSNLARYLAMSVEPFVGMTELEKSGISQRSNKLFTLSSIKQA
                     TRALLGKDPKEDNFEECTHIATQYWQAIFSVMPDWQLAAKKEVSPVQLRQDYVHAHGI
                     GLQALGQLGYTLLTDYPEQWPEKIAALKTFNWRRNNPDLIKRAMQHGKLSKTSTAIQL
                     TCNALKTALSIPLTPEEQELEAQMVVS"
     gene            15038..16660
                     /gene="dndC"
                     /locus_tag="LGK42_002654"
     CDS             15038..16660
                     /gene="dndC"
                     /locus_tag="LGK42_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBK2658887.1"
                     /translation="MSKLVQAYDLAEYEDFINQEQFAGRPLAEYVAEVQRIYCADKRP
                     WVIGYSGGKDSSAVITLVYLALLGLPPEMRSKDIFVVSSDTLVETPVVVDLIKKTMLQ
                     IEAGAKRNGLPITQHAVTPKTNETFWVNLLGKGYPAPTRSFRWCTERMKINPVSDFIK
                     DKVSQFDEVIVVLGSRSSESASRAQVIAKHKIDGSRLARHTTLANAFIYTPIDTWDVE
                     DVWKLLRGAFRYAPEDIDEWESPWGGNNRPLWTLYMDSSAQGECPLVIDDSTPSCGNS
                     RFGCWTCTVVTKDKAMESLIKNGEEWMSPLLKYRDLLAFTTDPVNKDTFRNYKRRTGK
                     VSYQYAKESDEPGAERKHVPGPYWLKYRQQWLKELLEIERDLNAQGHTITLITKPELH
                     AIRQEWLKDPNEPDWYDTLPGIYREVYQQDLDWVVDDQSRFDASDADLLVQIAQGFDV
                     VPEMVMKLIELEVSMEGLSRRQGIFDKLGTILKQDWGTLEEIEQQQVTLQKRVERDIH
                     QEELTKLETELQVLQRRIVDASESSLLIGEEK"
     gene            16662..18662
                     /gene="dndD"
                     /locus_tag="LGK42_002655"
     CDS             16662..18662
                     /gene="dndD"
                     /locus_tag="LGK42_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HBK2658888.1"
                     /translation="MLIKQLVLHNFRVFNGTHTIDLAPRKRPHDLNPRPIVLFGGLNG
                     AGKTSILSAIRIALYGRLAFGLATQQQEYIEQLSALIHNGAYYIEQPEEASVELTFTY
                     NKGGHEAEFTVTRTWKKGKRDRLSLQQDGQPLSELDYDQCQGFLNELIPHGIADLFFF
                     DGEKIAELAEDESGNILRTAVRRLLGLDLISKLRNDLMIFVKRQQSSQLASSQQQQIA
                     ELEKQSKELACQTEALLESADFAKSRIEFLSKDITHYEGLLNAQGGAFAQTKAQEKQK
                     VETLLKDKEHLEKALRQECDGSLPYALAPTILSHLLQQIADETQIKQAKSFEKELSQF
                     LTQLRNDIAFRSAGTGKIAAEAITDNLKDYMATKPKGDLLFDISEREAGMLQQSIEID
                     SKRAWQRFELYRTQLSEIEQQLEQAAANIARAPEDEQLMDLFEKLRDLDRQREAQLQK
                     YRSLLEDAKRKKQQQLDCVRQIQKAHDAARYQHSYSSAFKNAQETINLLDRYSDVLTQ
                     ARVKTLSTNFELAYRKLARKEDLQLSAHINPQTFDVELIDENGSVINRKLLSAGEKQI
                     YAIAILEALAKTSGRDLPVIIDTPLGRLDSQHRDKLINHYFPEASHQVVLLSTDTEVD
                     ERYFVDQLRDDISHAYEIVFNAHTKSSTLKPGYFWELTKEAI"
     gene            18662..19015
                     /gene="dndE"
                     /locus_tag="LGK42_002656"
     CDS             18662..19015
                     /gene="dndE"
                     /locus_tag="LGK42_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndE"
                     /protein_id="HBK2658889.1"
                     /translation="MLPNRMALSRQTEDQLKKLKGYTGITPNIAARLAFFRSVESEFR
                     YSPERDSKKLDGTLVLDKITWLGETLQATELVLKMLYPQLEQKALIKAWAAHVEDGIA
                     ALRNHKSLISLLNAI"
BASE COUNT         4574 a         4967 c         5174 g         4509 t
ORIGIN      
        1 gcgaagcaca accgacagga cgttttgtgc tgtgtgtcct gtggatgccc gttaacactt
       61 cagcggggca atgacggaca accaccgtgg tttgaacatg accagatgac tgtcgctgaa
      121 aaaatcctgc tgcgatgtac ctggcttgac ccggcggaga aagaggcccg tcgtttgcat
      181 ctgcagggca tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg
      241 tgtgacgaag attatgaggg ggaaaagtgc tgcccacgct gcggtaccgg ggtatacagc
      301 agggcgtggg ggcggcagga ggtgccgtcg gaagatgcca gggctgataa tccgttacaa
      361 aggctgtaat ggttgcctcc ggagcagttt gcggtgatgc ctttcccggg aatggattgc
      421 cagtgcgggg actgtggagc ttaattccgg tgttggtggc ctttccgttg attttatgcc
      481 aacagccccc tgtgactgac aggctgcctc gtcattccat tcgtatctcc agtaacaggg
      541 ggttgtattg tattttattg tgccggttca ggtgtgcgtt tcccggcgcg tctgcaccgg
      601 cttaaccaaa ttcaacaggg ataaaatagt ggtaagcgta cagcctgaac cgtctggtca
      661 gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac caaagtgtca
      721 gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct gccctaatta tcctcccgaa
      781 tttaaacagc tgctcgttgc tgcctcctgt gaacccggga tatccatctc aaaacttgct
      841 cttgaaaatg gcattaacgc caatctgttg ttcaaatggc gacaacaatg gcgcgaggga
      901 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
      961 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgtgaggta
     1021 acgttccggc acgggacgct ccgcttcaat ggcaatgtca gcgaaaagct cctgactctg
     1081 ctgatacagg aactgaagcg atgatcccgt taccttccgg gaccaaaatt tggctggttg
     1141 ccggtatcac cgatatgaga aatggcttca acggcctggc tgcgaaagta caaacggcgc
     1201 tgaaagacga tcccatgtcc ggccatgttt tcattttccg gggccgcagc ggcagtcagg
     1261 ttaaactgct gtggtccacc ggtgacggac tgtgcctcct gaccaaacgg ctggagcgtg
     1321 ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg
     1381 cgatgctgct ggaaggtatc gactggcgac agcccaagcg gctgctgacc tccctgacca
     1441 tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata cgggcttatg
     1501 aacgacatct cttctgacga catcttcctg ctaaaacagc gcctggccga acaggaagcg
     1561 ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga ccatctgcag
     1621 gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc
     1681 cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga tacgctgact
     1741 ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg
     1801 ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcggcgcc gtgctgcccg
     1861 aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct ggagttgatg
     1921 cgtagcgcat tccgggttat ccggacggta cgggaaaaac atgcctgtac tcagtgcgat
     1981 gccatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc cggaccgggg
     2041 ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta ttgccagtca
     2101 gaaatatacg gccggcaagg tgtggagctg agccgttcac tgctgtcggg ctgggtggat
     2161 gcatgctgcc ggctgctgtc tccgctggaa gaggcgcttc atggctatgt catgactgac
     2221 ggcaaactcc atgccgatga taccccggtc caggtactgc tgccgggtaa taagaagacg
     2281 aggaccgggc ggttgtgggc gtatgttcgt gatgaccgca atgccgggtc agcgttggca
     2341 cctgcagtgt ggttcgctta cagcccggac agaaaaggca tccatccgca gactcatctt
     2401 gcttgcttca gcggtgtgct gcaagcggat gcgtacgccg ggttcaacga gctgtatcgc
     2461 aatggtggga taacggaagc tgcctgctgg gctcatgccc gccgaaagat ccacgatgtg
     2521 cacgtccgca tcccgtcagc actgacggaa gaagccctgg agcagatcgg tcagttgtac
     2581 gccatagagg cggatataag gggaatgccg gcagagcagc ggcttgctga acgtcagcga
     2641 aaaacgaaac cgctgttgaa atccctggaa agctggttgc gtgaaaagat gaagaccctg
     2701 ttcttcggct ctggccatgg tggtgagcgg ggagcgctac tgtacagcct gatcgggacg
     2761 tgcaaactga atgacgtgga tccagaaagc taccttcgcc atgtgcctgg cgtcatagca
     2821 gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact gccagctgaa
     2881 taacacatcc ccgtcaatac ggccctcgct gtacgcttac ttttgttcgg actgcttatg
     2941 gcctttttcc actggtgtag caggaaagtt aaccggctgc caccatgaga tgacgtgtaa
     3001 accgtgcagg catgttttca cgtggtgacg ctttcattgg gggtctacgc cggaacccca
     3061 atattgttca catacatgaa tttgaaaaac taagcgatac tgctgtccgt accttataaa
     3121 cttatttcaa cgcttccggc agcgtcacaa tccacagttc actgtcagac tccggtttct
     3181 ccagtggctt cactctgagg gtcgtttcaa cctgcttgtc gtcaagcgtc agctttcctt
     3241 ccgcgttcac ccgataatcc ttgaatgtct tgccctcgac cggatgttgc atcgccacct
     3301 tcacgccaaa gtcgttatcg ttcgccaccg ccagcgtttt gctgtcgatc agcgccaacc
     3361 cttcagcctt ctcctgctgc cagcccaagg agcgcagatc aacaacctgc gttttctgcg
     3421 caagggtaat gccgcgctgt gacagggttt tctcatcatc aaactccgga tactcacccg
     3481 gcttgtcgaa gcctgacagg tcgctcgcct tattgagatc gacctcgtaa atcaggttac
     3541 gcattctgtt gtttttatct cttccctgtt cgatcagcag gatgtgctgg ttatcgagcg
     3601 ccacgatgtc tccgatttta gcgtcgctgt ttttgctgta ggccgcgctg tcgataggat
     3661 aaccgtacat cgcggttttt ccgcttgccg gatcgaagct caccagacgc gtaaacagcg
     3721 cctttttctt gctcttagcg tcgatatcca gcgtactttg cacggcgaca ataatacgcc
     3781 cgtccggcat acgggtgagg ccttcaaagc cgcggttcgc ctgacgccat ttgaggatat
     3841 ttggcaaacc gcccgcaatc gctttttccc cttccgccgc ctgcggaccg tggattgcca
     3901 ggattttccc tttactgtcg atgttaatca ggaacgggcc atactcatcg cacagccagt
     3961 aacctccttt accatccgga gtgatccctt ccgtatccag cccgcgatta tcccccttca
     4021 gtctatgtaa cgtgtcgctg aaggccactt cattggtgga gccaatcacg tcgcttgcca
     4081 gcggcagacc gttgatcgcg cctttatcgt catgcagagg acggggatcg atggcctccg
     4141 ctttgccgtt ttgtacacga atcgtcatca gcagcggcgc gaaatatggc gtaacgaaaa
     4201 ttttagtttc gtttttcccc tctttcggtg aatccgcgtt cggaccgcga tcggtaatgg
     4261 ttgcgaatgt cagggcatcg ccctgtttac cggtgaacag caggccagaa cctatcccaa
     4321 ccggaaggcc gttcgggaat gcactggcaa atgcgccggc gtagttcacg tgcgtgcctt
     4381 ctggaaaact gacgacatag cgctcagccg taggttgtgc cgccagggca gagaaagaga
     4441 gcgtacaacc gattagcacg ggaataattt ttcttttcat attgttagca tccataaagt
     4501 gaagatcaca ctacaccata aagaagaaac gagtcaggaa aatgacatcg aatggcaaca
     4561 tgcgcgggcc tgaggccaaa gaaatgtaaa acactgttgt tatttgtaac aacggaaaca
     4621 tttacggaaa ttatttgtct ttcttcgtga tcaaaaatgc tgtgtcttgt tggattggag
     4681 tgatgagggc tgcgtttagg aaacgcccta tattgtacgg ttgcattatc gcggtagggg
     4741 taatgcacgt gtcagtatga tgagctgacc acactcacgt gggtggactg gtataacaat
     4801 cgacgattgc tggaaaggca gggccatatc ccaccggcag aagcagaaaa agcttattat
     4861 gcttccatcg gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
     4921 acccggggcg gttcacttag ccgcgtgtcc attattgctg gataagatca gtcaataata
     4981 gcagaccaac cacatcatga gtcattctga ccgacacatg ccagaagcag aggctccatc
     5041 ccattttata cctaccttat aacacttaga aaaacaacat gttaaaaatg tctattggaa
     5101 acaattttat ttccaattgt aatgataacc attctcatat taatatgact acgtgataat
     5161 taacttttga tgcactccgc atgtctgacc gcgccactac cacagcttcc ttaacgttcg
     5221 agtcgcttta tggcacacat cacggctggt tgaaaagctg gctgacgcgc aaactccagt
     5281 ctgcttttga tgcagatgac attgcccagg acactttttt gcgggtaatg gtcagcgaaa
     5341 cgctctcgac gatccgcgat cctcgctcct tcctctgcac tatcgccaaa cgcgtgatgg
     5401 tggacctgtt tcgccgaaac gcgctggaaa aagcgtatct ggagatgctg gcgcttatgc
     5461 cggagggggg agcgtcttca cctgaggaac gcgaaagcca actcgagacc ctacaactcc
     5521 tcgacagcat gctggacggg ctaaacggca aaacacgtga agcgtttctg ctttcgcaac
     5581 tggatggtct gacatacagc gagattgcgc acaaactcgg tgtttccatc agctccgtga
     5641 aaaaatacgt ggcgaaagcc gtcgagcact gcctgctgtt ccgtctggag tatgggttat
     5701 gaatcctttg ttaaccgatt cccgccgtca ggcgctgcgt tcagcttccc actggtatgc
     5761 cgtgctaagc agcgagcgcg tcagcccaca acaggaagcg cgctggcaac agtggtatga
     5821 acaggatcag gataaccagt gggcctggca gcaggttgaa aacctgcgca accagcttgg
     5881 cggtgtgcct ggcgacgttg ccagccgggc gttgcacgat acccgcctca cccgccgtca
     5941 cgtgatgaaa ggattactgc tgttgctcgg cgctggcgga ggctggcagc tctggcagtc
     6001 ggaaaccagc gaaggtctgc gggcagatta ccgcaccgcc aaaggcacag tcagccgtca
     6061 gcaactggaa gatggctccc tgctcacgct gaatacccaa agcgcggcgg atgtgcgttt
     6121 tgatgcgcat cagcgcaccg tccggctctg gtacggtgaa atcgccatta ctaccgcgaa
     6181 agatgccctg caacgcccct tccgtgtcct gacccgtcag ggccagctca ctgctttagg
     6241 gacagaattt accgtccgcc agctggataa tttcacgcag cttgacgttc agcagcacgc
     6301 tgtggaagtg cttctcgcca gtgcccccgc gcaaaaacgc atcgtgaacg ctggtgaaag
     6361 cctgcagttc agcgcctctg agtttggcgc agtgaaaccg ctggatgacg agagtacaag
     6421 ctggacgaag gacatcctga gcttcagcga taaaccgctg ggtgaggtga tagccacgct
     6481 aacccgttac cgcaacggcg tgctgcgctg cgatcccgcc gttgccgggc tgcgcctgag
     6541 cgggacgttc ccgctgaaaa ataccgatgc gatcctgaac gttatcgcgc aaacgcttcc
     6601 cgttaaaatt cagtctatta cgcggtactg gataaacatt tcaccactgt aaggaaaata
     6661 attcttattt cgattgtcct ttttaccctt ctcgttcgac tcatagctga acacaacaaa
     6721 aatgatgatg gggaaggtat gacgccgtta cgcgtttttc gtaaaacaac acctttggtt
     6781 aacgccattc gcctgagcct gctgccgctg gccggtctct cgttttccgc ttttgctgca
     6841 caggttaata tcgcaccggg atcgctcgat aaagcgctca atcagtatgc cgcacacagc
     6901 ggatttaccc tctcggttga cgccagcctg acgcgcggca agcagagcaa cggcctgcac
     6961 ggcgattacg acgtcgagag cggcctgcaa caactgctgg acggcagcgg actgcaggta
     7021 aaaccgctgg gaaataacag ctggacgctg gagcccgcgc ccgcaccaaa agaagatgcc
     7081 ctgaccgtgg tcggcgactg gctgggtgat gcgcgtgaaa acgacgtatt tgaacatgct
     7141 ggcgcgcgtg acgtgatccg ccgtgaggat ttcgccaaaa ccggcgcaac caccatgcgt
     7201 gaggtactta accgcatccc tggcgtcagc gcgccggaaa acaacggcac cggcagccac
     7261 gacctggcga tgaactttgg catccggggc ctgaacccgc gcctcgccag ccgctcgacc
     7321 gtcctgatgg acggcatccc cgtccccttc gccccttacg gtcagccgca gctttcactg
     7381 gctcccgttt cgctcggcaa catggatgcc attgacgtgg tacgcggtgg tggtgcggtg
     7441 cgttacggac cgcagagcgt gggcggcgtg gtgaactttg ttacccgtgc cattccgcag
     7501 gactttggta tcgaggcggg cgtggaaggt cagctcagcc caacctcttc acaaaacaac
     7561 ccgaaagaga cgcacaacct gatggtgggc ggcacagcgg acaacggttt tggcaccgcg
     7621 ctgctctact ccggcacgcg cggcagtgac tggcgcgagc acagcgccac ccgcatcgac
     7681 gacctgatgc tgaaaagcaa atatgcgccg gatgaggtgc acaccttcaa cagcctgctg
     7741 caatattacg acggtgaagc cgacatgccc ggtggcctgt ctcgcgcgga ttacgacgcc
     7801 aatcgctggc aatccacccg cccgtatgac cgcttctggg gtcgtcgcaa gctggcgagc
     7861 ctgggctacc agttccagcc agacagccag cataaattca acattcaggg gttctacacc
     7921 caaaccctgc gcagcggcta cctggagcaa ggcaaacgca tcaccctctc gccgcgtaac
     7981 tactgggtgc gcggtattga gccacgctac agccagatct ttatgatcgg cccttccgcg
     8041 cacgaagtgg gcgtgggcta tcgctatgtg aatgaatcaa cgcatgaaat gcgttactac
     8101 accgccacca gcagcgggca gttgccgtcc ggctcaagcc cttacgaccg cgatacgcgt
     8161 tccggcaccg aggcgcacgc ctggtatctg gatgacaaaa tcgacatcgg caactggacc
     8221 atcacgccgg gtatgcgttt cgaacatatc gagtcatacc agaacaacgc catcacaggc
     8281 acgcacgaag aagtgagcta taacgcaccg cttccggcgt tgaacgtgct ctatcacctg
     8341 actgacagct ggaatcttta tgcaaacact gaaggctcgt tcggcaccgt acagtacagc
     8401 cagattggca aggctgtgca aagcggcaat gtggaaccgg aaaaagcgcg aacctgggaa
     8461 ctcggtaccc gctacgacga cggcgcgctg acggcggaaa tggggctgtt cctgattaac
     8521 tttaacaatc agtacgactc caaccagacc aacgacaccg tcactgcacg tggcaaaacg
     8581 cgccataccg ggctggaaac gcaggcacgt tacgatctgg gtacgctaac gccaacgctt
     8641 gataacgttt ccatctacgc cagctatgcg tatgtgaacg cggaaatccg cgagaaaggc
     8701 gacacctacg gcaatctggt accattctcc ccgaaacata aaggcacgct gggcgtggac
     8761 tacaagccag gaaactggac gttcaatctg aacagcgatt tccagtccag ccagtttgcg
     8821 gataacgcca atacggtgaa agagagcgcc gacggcagta ccggccgcat tcccggcttc
     8881 atgctctggg gcgcacgcgt ggcgtatgac tttggcccgc agatggcaga tctgaacctg
     8941 gcattcggtg tgaaaaacat cttcgaccag gactacttca tccgctctta tgacgacaac
     9001 aacaaaggca tctatgcagg ccagccgcgc acgctgtata tgcaggggtc gttgaagttc
     9061 tgatgtgtta acgcccggct tgccgggctt ttagctggaa tgtgattatg ttggcattta
     9121 tccgttttgt ttttgcaggc ctgctgctgg tgatcagcca cgcctttgcc gccacggttc
     9181 aggacgaaca cggcacgttt acactcgaaa aaacgccaca acggattgtg gtgctggaac
     9241 tctcgttcgc cgatgcgctg gccgccgtgg acgtcagccc gatcggtatt gccgacgata
     9301 acgatgcaaa acgcatcctg cccgaagtgc gtgcgcacct gaaaccgtgg cagtccgtcg
     9361 gaacgcgcgc gcagccgagc ctggaagcca ttgccgctct gaaaccagac ctgatcattg
     9421 ccgacagcag tcgccatgcg ggggtttaca tcgccttgca gcaaatcgcg ccggtactgc
     9481 tgcttaagtc ccgcaacgaa acctacgctg aaaatttgca atctgcggct atcatcggcg
     9541 aaatggtggg taaaaagcga gagatgcagg cacgcctgga acaacataaa gagaggatgg
     9601 cgcagtgggc cagccagctt cccaaaggga cacgcgtggc ctttggcaca tcacgcgaac
     9661 agcaattcaa cctgcatact caggagacct ggaccggcag cgtgctggcc tctctggggc
     9721 tgaacgttcc cgctgcgatg gcgggtgcgt ccatgccgtc catcggcctg gagcaactgc
     9781 tggcggtcaa tcctgcctgg ctgctggttg cccactatcg cgaagagagc attgttaaac
     9841 gctggcaaca agatccgctc tggcagatgt taaccgccgc gcagaagcag caggttgctt
     9901 cggtcgacag taacacctgg gcgcggatgc gcggtatttt tgctgcagag cgtattgccg
     9961 ctgacacggt aaaaatcttc caccatcagc cgcttaccgt tgtgaaatga ccgcgataaa
    10021 acacccggtg ctgctgtggg ggcttcccgt tgcagcactt attattattt tctggctgag
    10081 tctgttttgc tactcggcca ttcctgtttc cggagcagat gcaacccgcg ccctgctgcc
    10141 tggacacacg ccaacgctac cagaagcgct ggtgcaaaac cttcgtttgc cacgaagcct
    10201 ggtcgccgtt ctgatcggcg caagcctggc gctcgcgggc acgctgctgc aaaccctgac
    10261 ccacaaccca atggcctctc cttcactgct cggcattaac agcggcgcgg cgctggctat
    10321 ggcgcttacc agcgcgctga gtccgacgcc gattgcaggc tattctctgt cgttcatcgc
    10381 ggcatgcggg ggcggcgtga gctggctgct ggtcatgacc gcaggaggcg gatttcgtca
    10441 tacccatgac agaaacaaac tgatcctcgc gggtatcgcg ctgtcggcct tttgtatggg
    10501 ccttacccgc atcaccctgc tgctggccga agatcatgct tacggcatct tttactggct
    10561 ggcaggcgga gtgtcccacg cccgctggca ggatgtctgg cagctcttgc cggtggtggt
    10621 cactgcagtc cctgtcgtgt tgctgctggc gaatcaactg aacctgctca acctcagcga
    10681 cagcaccgcc catacgctgg gagtgaacct gacgaggcta cgtttggtca tcaatatgtt
    10741 agtgctgctt ctggttggtg cgtgcgtcag cgtggcaggt ccggtggcgt ttatcggtct
    10801 gctggtgcca catctggcgc gcttctgggc aggcttcgat cagcgcaacg tactgccagt
    10861 gagcatgctg ctgggggcca cgctgatgct gctggcagat gtactcgcac gcgcgctggc
    10921 cttccccgga gatctgcccg caggcgcagt gctggcgctg attggcagcc cttgctttgt
    10981 ctggcttgtg aggaggcgag gatgaaaatt gcgctggtta ttttcatcac ccttgccctg
    11041 gcgggctgtg cgctgttatc actccatatg ggagtgatcc ccgtgccgtg gcgcgcgctg
    11101 ctgaccgact ggcaggccgg acgcgagcat tattatgtat tgatggagta ccgactgccg
    11161 cgcttgctgc tggcactgtt tgtcggtgca gccctcgccg tggcgggcgt gctgatacag
    11221 gggattgtgc gcaaccctct ggcatcaccg gatattctcg gcgttaacca tgccgccagc
    11281 ctggcctctg tgggggctct acttcttatg ccgtcactgc ccgtgatggt gctgccgctg
    11341 ctggcctttg cgggcggcat ggcggggttg atattgctga agatgctggc aaagacccac
    11401 cagccgatga agctggcgct caccggcgtg gcgctttctg catgctgggc cagcctgacg
    11461 gattatctga tgctctcgcg cccgcaggat gtgaacaacg ccctgctgtg gctgaccggc
    11521 agcttatggg gccgtgactg gagctttgtg aagattgcca tcccgctgat gattttattt
    11581 ctgccgctga gcctgagttt ttgccgcgat ctcgacctcc ttgcactcgg cgatgcgcgc
    11641 gccaccacgc tcggtgtgtc ggtgccccat acccgattct gggctttgtt actagctgtc
    11701 gccatgacat ctaccggcgt ggccgcctgc ggcccgatta gctttattgg tctcgtggtg
    11761 ccgcatatga tgcgtagcat caccggtgga cgtcaccgca gactgctgcc tgtttcagcc
    11821 ctgacaggtg cgttgctgtt ggtggttgcc gatctgctgg cgagaattat tcatccccca
    11881 ctggagctcc cggttggcgt gctgaccgcc attatcggtg cgccgtggtt tgtctggttg
    11941 cttgtgagaa tgcgataaat gactttacga actgaaaatc tgacggtcag ttacgggaca
    12001 gacaaggtac ttaacgacgt ttcactctca ctgccaacgg ggaagatcac cgccctgatc
    12061 ggtcctaacg gttgcgggaa atcgacgctg ttaaactgtt tttcgcggct tttaatgccg
    12121 cagtctggca ccgtatttct cggcgataat cccataaata tgctctcatc gcgccagttg
    12181 gcccgcaggc tttcgctgct gcctcagcac catttaacgc cagaggggat cacagtccaa
    12241 gagctggttt cgtatggtcg taatccctgg ctgtcactct gggggcgtct ctccgctgaa
    12301 gacaatgcac gagttaatgt cgccatgaac cagacccgga tcaatcatct tgccggtcgt
    12361 cggttaaccg agctttccgg cggtcagcgc cagcgcgtat ttctggcgat ggtcctggcc
    12421 cagaatacgc ccgttgtatt acttgatgag ccaaccacct atcttgatat caatcaccag
    12481 gtggacctga tgcggttgat gggcgaactc cggactcagg ggaaaacggt ggtcgctgtg
    12541 ctgcacgacc ttaatcaggc tagccggtac tgcgatcaac tggtggtaat ggcaaacgga
    12601 catgttatgg cgcaaggcac accagaagag gtgatgaccc caggattgct gagaacagta
    12661 ttcagcgtgg aagcggaaat acaccccgag ccggtatctg gcaggccgat gtgcctaatg
    12721 aggtagattg cacaggccgt aagaaccaaa ccacgactga atgaaactgg actggcgcca
    12781 gcaagcctgt tcagactggg gctgaacttt tccggactct gaaagattac caatactcat
    12841 cgtccatccg cttgctttag gctgacaggt tcataatcaa cgcaaaccag agctgtacag
    12901 gcttgggcgc ggctttcaaa ccagtcgtga tcacggcaat caattttgaa ctctgcttaa
    12961 cggacatttc tgtataaccc ttacggcaac gaaaaacgcg aagttaaaat tttagaaacc
    13021 caaaaacgtg acatgactaa gtttagattt caggggggga gatcaaaaaa tttcgctctg
    13081 tgccagagcg gacattcacg gagctggttc attaccaatg aggttgggct tttgaggata
    13141 aatcaatgat cagacgccaa cgtaaatcaa aagcaaccct ggaacggaat tgctaatcca
    13201 gtttctgacc atcgattttt ctaaaaagtg tgcgtttgct actacttagg taggtgcagc
    13261 tttcttaatc accggcagcc acgttataca ggccagttga tggatcgatt gttatcaatg
    13321 atatcttcat gagtcggtgt ctcacccagc ttaccgaagc tggcataaag tgaaggcaga
    13381 cgggcccgtc cttctccctt tttcgccaga gggaaagcgc gaagcatggt ggcagcctcc
    13441 cagtcacaac aataggatgg tgtgcacggc tgctgacgcc atgattcagc gatagagccg
    13501 gaaaatacgg ggtcaaagcc ggtatcatta accagagtca tcgtgacctg tactgcggct
    13561 ggatgatctc ctgctactgc aactgctagg cgaccgcggc tcccttcagg tagtgaagtg
    13621 gtcaacttaa actggccacc gatttagagt ttttccagta gcggtgttcc gattcgtttg
    13681 ggggcaaccc gccgttattg tcatgcggtc ttagcgagct gtaatacccg atgatatagt
    13741 ctgtgatcgc atgagcagct tcgctaaagc ttatgcgtta gcgctctaca gaagtacaga
    13801 gcatgtctgc acggttgctc tgggttatag agcaaaattg cgggtatatt gaaaggtggt
    13861 acgcaattta ttacgtgaaa aagctgtaat ttctagccag tgttacgtaa aatataacgt
    13921 aacaaaattc accttatgct tttatggatt acagcatggc tagtgttgat gcagattact
    13981 gctactcgtt tccggctata cgtggcattc aggctggtcg ccctttctat atcgccacat
    14041 gcccgatgcg tatcatcccg aaaatattca gttttgatga aaatgatgtg ccaccggaat
    14101 tacgcgcaca gcgcacgcta aataaatcgc gcattcccga gatggtacgt tatttgttgg
    14161 aaaaccctaa agattatgtg ttttcagctc tgacggcttc cattgccgta gacgttcaat
    14221 ttgatgagtt tccaggttct aacaatctgg ggacgttgtg cgtaccaatg gatgcgcaaa
    14281 ttctaatcaa tgatggacaa catcgccgta aagcgattga agacgcttta gacgctcgtc
    14341 cggagctggg ccaggataat atcccggtgt tgttctttgt cgatgaaggc ttaaagcgta
    14401 gccagcagat gttcgccgat ctgaataaat acgccattcg ccccagccca tcgctggcgt
    14461 cgctatacga tcatcgcgat gcctgttcga atctggcgcg ttatctggca atgtcagtag
    14521 agccgtttgt tgggatgacg gaactggaaa aatctggcat tagccagagg tcgaataagc
    14581 tatttacgtt gagcagcatc aagcaggcta cccgcgcatt gttgggcaaa gatcccaaag
    14641 aggataactt tgaggaatgc acccacattg ctacccaata ttggcaggct atttttagtg
    14701 taatgcctga ttggcaatta gcggctaaaa aggaagtttc accggttcaa ttgcgacaag
    14761 attatgttca cgctcatggc attggattgc aggcacttgg gcaactagga tataccctgc
    14821 tgacggatta tcctgagcaa tggccggaga aaatcgctgc actcaaaaca tttaactggc
    14881 gtagaaacaa tccggacctc atcaagcgag cgatgcagca tgggaagttg agtaaaacca
    14941 gtaccgcgat ccagttaacc tgcaatgcgc taaaaaccgc actctcaatt cccctcactc
    15001 ctgaagaaca ggagcttgaa gctcaaatgg ttgtctcatg agtaaattag ttcaggccta
    15061 tgatttggcc gaatacgaag attttattaa tcaggaacag ttcgccggac gtcctctggc
    15121 cgaatatgtt gctgaggttc aacgaattta ttgtgcggat aaacgtccgt gggtgattgg
    15181 ctacagcggt gggaaagatt cttccgctgt gatcacgctg gtgtatctgg ccttgctcgg
    15241 cctgcccccg gaaatgcgca gcaaagatat ttttgtggtg tcgtccgata cgctggtcga
    15301 aacaccggta gtcgtggatc tgattaagaa aacgatgttg cagattgagg cgggagcgaa
    15361 gcgtaacggc ttgccgatca ctcaacacgc ggtaacgcct aaaacgaatg aaaccttctg
    15421 ggtcaatttg ctggggaaag gttatccggc tccaactcgt agtttccgtt ggtgcactga
    15481 gcgcatgaaa atcaacccgg tgagtgactt tattaaagat aaggtcagcc agtttgatga
    15541 agtcatcgtc gtacttggtt cgcgcagtag tgaaagtgct tctcgtgcgc aggtgatcgc
    15601 aaagcacaag attgatggat cacgccttgc ccggcatacc acgctggcaa atgcattcat
    15661 ctataccccc attgatacct gggatgtaga agatgtctgg aagctgctgc ggggcgcgtt
    15721 ccgctatgcc cctgaagata tcgacgagtg ggaaagcccg tggggtggaa acaaccgtcc
    15781 actgtggacg ttgtatatgg attcttccgc acagggtgaa tgtcctctgg taattgatga
    15841 tagtacccct tcctgcggca actcgcgttt tggctgttgg acctgtaccg ttgtcactaa
    15901 agataaggcg atggaaagcc tgatcaaaaa cggtgaagaa tggatgtctc cgttgctgaa
    15961 atatcgtgat ctgctggcat ttaccaccga tccggtgaat aaagacacgt ttcgaaacta
    16021 taagcgccgt actggtaagg tgagctatca gtacgctaaa gaaagtgatg agccgggggc
    16081 tgagcgtaag catgttccag gtccttactg gctaaaatac cgtcagcaat ggctcaaaga
    16141 attgctggag atagagcgcg atttgaatgc tcaaggccat accattacgt taattaccaa
    16201 accagagctg catgcgattc gtcaggagtg gttgaaagat cctaatgaac cagactggta
    16261 tgacacgctg ccgggtattt accgtgaagt gtatcaacag gatttagact gggtggtgga
    16321 cgatcagtcg cgctttgatg ccagcgatgc agatctgttg gtgcagatag cgcagggatt
    16381 tgacgttgtg cctgaaatgg tgatgaaact catcgagctg gaagtttcaa tggaaggctt
    16441 aagccgtcgt cagggcattt ttgacaaact tggcacgatt ttaaagcagg actggggcac
    16501 tctcgaagaa attgaacaac aacaggtcac tctgcaaaaa cgagttgaac gagatattca
    16561 tcaggaagaa cttacaaagc tggaaactga acttcaggtt ttacagcgca gaattgttga
    16621 tgccagtgaa tcctcgttac tgattgggga ggagaaataa cgtgctcatt aaacagctgg
    16681 tattacataa ttttcgggta tttaacggta cacataccat tgatctggca cccagaaagc
    16741 gtccgcacga tttgaatcca cgacctattg tgctgtttgg tggtttaaac ggcgcaggga
    16801 aaacgtctat cctgtcggcg atccgcatag cgttgtatgg acgactggcg tttgggctag
    16861 cgactcaaca gcaagagtat atcgaacaac tcagtgcatt gattcataac ggcgcgtact
    16921 atattgagca gccggaagaa gcatctgtag aactgacgtt tacctataat aaaggcggtc
    16981 atgaggccga atttaccgtt acgcgaacct ggaaaaaggg gaagagggac cgcctttctc
    17041 tgcaacagga tgggcagcca ctcagtgagc tggattacga tcagtgccag ggatttctaa
    17101 atgagttaat tccgcatggc attgctgatt tgttcttttt cgatggcgag aaaattgcag
    17161 aacttgctga ggacgaatcc ggtaatattt tacgtactgc cgtacgacgc ttattggggc
    17221 tggatctcat ctctaaactg cgtaacgatt taatgatttt tgttaagcgt cagcaatcca
    17281 gccagctagc aagttctcag caacaacaga ttgcagagct tgaaaaacag agtaaagaat
    17341 tagcctgcca gacagaagca ctgctggaaa gcgccgattt tgccaaatcg cggattgagt
    17401 ttctatctaa agatataacc cattatgaag gcttgctaaa tgctcagggg ggcgcttttg
    17461 cccagactaa agcgcaggaa aaacagaaag tcgaaacgct gctaaaagat aaagaacatc
    17521 tggaaaaagc actgagacaa gaatgcgatg gctcgctacc ttatgcgctt gcgcccacta
    17581 ttttgtctca tttgctgcaa caaattgctg atgaaacgca aattaaacag gcaaaaagct
    17641 ttgaaaaaga gctgagccag tttttaacgc aactcagaaa tgatattgca tttcgttctg
    17701 cggggactgg caaaatagca gctgaagcga ttaccgataa tctaaaagat tatatggcaa
    17761 cgaaaccaaa aggtgatttg ctctttgata tctctgagcg tgaagcaggt atgcttcaac
    17821 agtctattga gatagatagt aaaagagcat ggcagcgttt cgagctttat cgcacacagt
    17881 tatctgagat tgaacaacag ttagaacaag cggctgctaa tattgcccgc gctcctgaag
    17941 atgagcagct gatggatctc tttgaaaaat tacgtgacct ggataggcag cgcgaagccc
    18001 aattgcagaa atatcggtca ttgctagaag atgctaaacg taaaaaacag cagcaactgg
    18061 attgtgttcg tcagattcaa aaagcgcatg acgccgctcg ttatcagcac agttatagca
    18121 gtgcgtttaa gaatgctcag gaaacgataa atttactcga tcgctacagc gatgtattaa
    18181 cccaggctcg ggtaaaaacg ctgtcgacaa acttcgagct tgcttaccgt aagttggctc
    18241 gcaaagagga tttacaactt agtgcgcata tcaaccctca aacgtttgat gttgaattaa
    18301 ttgatgagaa tggctcagta attaaccgta agttactttc tgctggtgaa aaacagattt
    18361 atgcaattgc tattcttgag gcgctggcga aaacctctgg ccgtgattta ccagtgatca
    18421 ttgatacacc attgggtcgt ctggactctc aacatcgaga taaattgatt aatcactact
    18481 tccctgaagc cagtcatcag gttgtgttgc tttctaccga tactgaagta gatgaacgtt
    18541 attttgttga tcagttgcgt gatgatatct ctcatgctta tgagattgtg tttaatgcac
    18601 atactaagtc atcaacgctg aagcctggtt atttctggga attaactaag gaggctatct
    18661 gatgctcccg aatcgaatgg cgcttagccg tcagactgaa gatcagctta aaaagcttaa
    18721 agggtatact gggattacac ccaatattgc tgcccggcta gcatttttcc gctcggtgga
    18781 gagtgagttt cgctattcac ctgaacgaga tagtaagaag ctggatggta cgctggtgct
    18841 ggataaaatt acgtggctgg gggagacact acaagctaca gaattggtgc tgaagatgct
    18901 ttatccacaa ttagagcaaa aggcgttgat taaggcatgg gctgctcatg ttgaggatgg
    18961 gattgcggca ttgagaaatc ataaaagttt aattagctta ttaaatgcta tataaaacat
    19021 ttaaatttat tggtatgacc ttttgttttc aacctaaaac cacctcctag ggaggtggtg
    19081 attgagtttt ataattgtca tattgctaat tttaaaatta attcaccggc atcaggcgaa
    19141 tgtatcagcg tagctaaatg tgaaccacac ttgcaagaat tgaagttgct gtccacttta
    19201 aatttaaagc gtccgttagc aacc
//
LOCUS       DAFAVF010000076        19138 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFAVF010000076 DAFAVF010000000
VERSION     DAFAVF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.076"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..110
                     /locus_tag="LGK42_002657"
     CDS             <1..110
                     /locus_tag="LGK42_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HBK2658890.1"
                     /translation="PTIYYNPSTGYPMHGGFDSAGNTFGTRWQDYYDRQ"
     gene            142..873
                     /locus_tag="LGK42_002658"
     CDS             142..873
                     /locus_tag="LGK42_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBK2658891.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1090..3243
                     /gene="traD"
                     /locus_tag="LGK42_002659"
     CDS             1090..3243
                     /gene="traD"
                     /locus_tag="LGK42_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBK2658892.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGSEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEMNINVHRERGEDVEPGDDF"
     gene            complement(3252..3650)
                     /gene="vapC"
                     /locus_tag="LGK42_002660"
     CDS             complement(3252..3650)
                     /gene="vapC"
                     /locus_tag="LGK42_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBK2658893.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(3650..3877)
                     /gene="vapB"
                     /locus_tag="LGK42_002661"
     CDS             complement(3650..3877)
                     /gene="vapB"
                     /locus_tag="LGK42_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBK2658894.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            3959..9229
                     /gene="traI"
                     /locus_tag="LGK42_002662"
     CDS             3959..9229
                     /gene="traI"
                     /locus_tag="LGK42_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBK2658895.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHT
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAE
                     WMQRLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARTGIDEAISREQLIPLDREKGLFTSGIHVLNELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGLRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            9249..9995
                     /gene="traX"
                     /locus_tag="LGK42_002663"
     CDS             9249..9995
                     /gene="traX"
                     /locus_tag="LGK42_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBK2658896.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            10054..10914
                     /locus_tag="LGK42_002664"
     CDS             10054..10914
                     /locus_tag="LGK42_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK2658897.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            11017..11577
                     /locus_tag="LGK42_002665"
     CDS             11017..11577
                     /locus_tag="LGK42_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBK2658898.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAGRLEKIRRQNRTKAELQAVLDGK"
     gene            11713..11925
                     /locus_tag="LGK42_002666"
     CDS             11713..11925
                     /locus_tag="LGK42_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBK2658899.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            12171..12245
                     /locus_tag="LGK42_002667"
                     /pseudo
     CDS             12171..12245
                     /locus_tag="LGK42_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            12549..12755
                     /locus_tag="LGK42_002668"
     CDS             12549..12755
                     /locus_tag="LGK42_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBK2658900.1"
                     /translation="MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
     gene            13039..13296
                     /gene="repA"
                     /locus_tag="LGK42_002669"
     CDS             13039..13296
                     /gene="repA"
                     /locus_tag="LGK42_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBK2658901.1"
                     /translation="MSQTENAVTSSSGTKRTYRKGNPVPARERQRASLARRSNTHKAF
                     HAVIQARLKDRLSELADEEGITQAQMLEKLIESELKRRATS"
     gene            13528..13602
                     /gene="tap"
                     /locus_tag="LGK42_002670"
     CDS             13528..13602
                     /gene="tap"
                     /locus_tag="LGK42_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBK2658902.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCG"
     gene            13595..14077
                     /locus_tag="LGK42_002671"
     CDS             13595..14077
                     /locus_tag="LGK42_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658903.1"
                     /translation="MANHETHSVIAGGIESDLHIDSSKYPHPFCSLLQKRAHFDSFKH
                     LIFIRGLLAIAHGKRKNKISASMVSFYVSLVQVMCAHYIPDTNKVNLSVKELSVCSGG
                     SYTRVWRALKTLDNDLHLIAFDGGTIWFRPDMFETLRVGPDELVTARRRGNSVGGGHG
                     "
     gene            14070..14927
                     /gene="repA"
                     /locus_tag="LGK42_002672"
     CDS             14070..14927
                     /gene="repA"
                     /locus_tag="LGK42_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBK2658904.1"
                     /translation="MADLLQKYYSQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKDRMILSRNRN
                     YSRLATASP"
     gene            15627..15767
                     /locus_tag="LGK42_002673"
     CDS             15627..15767
                     /locus_tag="LGK42_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658905.1"
                     /translation="MAESGYSLAGLGMGKVKSINQYRLTPGFGGFTPVSYATTEYRLP
                     CR"
     gene            15891..16142
                     /locus_tag="LGK42_002674"
     CDS             15891..16142
                     /locus_tag="LGK42_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK2658906.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            16139..16426
                     /locus_tag="LGK42_002675"
     CDS             16139..16426
                     /locus_tag="LGK42_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2658907.1"
                     /translation="MTYTVKFRDDALKEWVKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            16596..17000
                     /locus_tag="LGK42_002676"
     CDS             16596..17000
                     /locus_tag="LGK42_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HBK2658908.1"
                     /translation="MTILVTAQTQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESAEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNREWSVQGHAWDDYVDNFFR"
     gene            17196..17984
                     /locus_tag="LGK42_002677"
                     /pseudo
     CDS             17196..17984
                     /locus_tag="LGK42_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18006..18341)
                     /locus_tag="LGK42_002678"
     CDS             complement(18006..18341)
                     /locus_tag="LGK42_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBK2658909.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTPLHFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSLIYILS
                     VIIKRLSVR"
     gene            18470..18817
                     /locus_tag="LGK42_002679"
     CDS             18470..18817
                     /locus_tag="LGK42_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658910.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYRGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPSWDDV"
     gene            complement(18837..>19138)
                     /locus_tag="LGK42_002680"
     CDS             complement(18837..>19138)
                     /locus_tag="LGK42_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658911.1"
                     /translation="RAEQRAAEVVVLPLVPATGLPADGTSATDLAVQLNGITYQACRG
                     DFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
BASE COUNT         4823 a         4518 c         5437 g         4360 t
ORIGIN      
        1 ccccaacgat ctactataac ccttcaaccg gatatcctat gcatggaggc tttgattcag
       61 cgggaaatac ttttgggact cgttggcagg actattatga caggcaataa gaaaactgta
      121 gtttgtaaaa aaggtaattt gatgaaaaca aaaaaattga tgatggttac actggtcagt
      181 tccactctgg ccctttcagg gtgtggtgcg atgagcacag caatcaagaa gcgtaacctt
      241 gaggtgaaga ctcagatgag tgagaccatc tggcttgaac ccgccagcga acgcacggta
      301 tttctgcaga tcaaaaacac gtctgataaa gacatgagtg ggctgcaggg caaaattgct
      361 gatgctgtga aagcaaaagg gtatcaggtg gtgacttctc cggataaagc ctactactgg
      421 attcaggcga atgtgctgaa ggccgataag atggatctgc gggagtctca gggatggctg
      481 aaccgtggtt atgaaggcgc agcagttggt gcagcgttag gtgccggtat caccggatat
      541 aattccaatt ctgccggtgc cacactcggt gtgggccttg ctgccggtct ggtgggtatg
      601 gctgcagatg cgatggtgga agatgtgaac tataccatga tcacggatgt gcagattgca
      661 gagcgtacta aggcaacggt gacaacggat aatgttgccg ccctgcgtca gggcacatca
      721 ggtgcgaaaa ttcagaccag tactgaaaca ggtaaccagc ataaatacca gacccgtgtg
      781 gtctcaaatg cgaacaaggt taacctgaaa tttgaagagg cgaagcctgt tctcgaagac
      841 cagctggcca aatcaatcgc aaatattctc tgattatctt ccggaggctg gtctgaccgg
      901 cctcctgact gacgcctcgc tttgctcgtt gtccaacccc ggggagaaat aaatatgaaa
      961 acactgagcg gttgtgcaaa ttctttgtgc gaccgtgaag tgttgccggc gaagccggaa
     1021 tttaacggtt attaatcttt tcatcattaa taatcagtcc gactgacttt ctttcttcag
     1081 aatatcatca tgagttttaa cgcaaaggat atgacccagg gcggtcagat tgcgtccatg
     1141 cgtatccgca tgttcagtca gatcgccaat atcatacttt actgcctgtt tatttttttc
     1201 tggatcctag ttggtctggt tttatgggta aaaataagct ggcagacgtt tgtgaatggc
     1261 tgcatttact ggtggtgcac cacgctggaa ggcatgcggg atttaatcag gtcccagccg
     1321 gtctatgaga tccagtatta cggcaaaacc ttccggatga acgcggctca ggtactgcat
     1381 gataaatata tgatctggtg cggtgagcag ttatggtcgg cattcgttct ggcgtctgtc
     1441 gtggccctgg ttatctgcct gatcaccttc tttgttgtct cctggattct ggggcgtcag
     1501 ggtaaacaac agagcgaaaa tgaaatcaca ggtggtcgtc agctgactga caatccgaaa
     1561 gaggtggcca ggatgctgaa aaaagatggc aaggattccg atatccggat tggcgatctg
     1621 ccgattatcc gggattctga aatccagaac ttctgtctgc acggcactgt cggggccggt
     1681 aagtcggagg ttatccgtcg tctggccaac tacgcccgtc agcgtggtga tatggtggtg
     1741 atttatgacc gttcggggga atttgttaaa agttactatg atccctccat cgataaaatc
     1801 ctgaatccgc tggatgcacg ctgtgctgcc tgggatttgt ggaaggagtg cctgacacag
     1861 ccagattttg ataataccgc aaatactctg atcccgatgg ggacaaaaga ggacccgttc
     1921 tggcagggtt caggacgtac catttttgcg gaagcggcat acctgatgcg taatgacccc
     1981 aaccgcagct acagcaaact ggtggacaca ctgctttcca tcaaaattga aaagctgcgt
     2041 accttcctgc gtaattcacc ggcggccaac ctggtggaag agaaaatcga gaaaacggcc
     2101 atatccatcc gtgctgtgct gaccaactac gtgaaagcca tccgttacct gcaggggatt
     2161 gagcataacg gtgagccctt caccatccgt gactggatgc gtggtgtccg ggaagatcag
     2221 aaaaacggct ggctgtttat ttcgtcgaac gccgacaccc atgcctccct gaagccggtg
     2281 atctccatgt ggctgtccat tgctattcgt ggtctgctgg caatgggaga aaaccgtaac
     2341 cgtcgtgtgt ggtttttctg tgacgagtta cccacgttac acaaactgcc ggacctggtg
     2401 gagatcctgc cggaagcccg taagttcggt ggctgttatg tgtttggtat ccagtcctat
     2461 gcccagctgg aagatatcta cggtgagaaa gcggctgcca cgctgtttga cgtcatgaac
     2521 acccgtgcct ttttccgttc tcccagccat aagattgcag agttcgctgc cggtgaaatt
     2581 ggtgagaaag agcacctgaa agccagcgag cagtattcct acggtgctga tccggtacgt
     2641 gacggggtat cgaccggtaa ggatatggag cgtcagacgc tggtcagtta ttccgacatt
     2701 cagtctctgc cggatctgac ctgttatgtc accctgcccg gaccgtatcc ggcagtaaaa
     2761 ctctccctga aatatcagac acgaccgaag gtcgctccgg agtttattcc gcgtgacatc
     2821 aacccggaaa tggagaaccg tctgagtgcc gtacttgccg caagggaagc agaaggtcgt
     2881 cagatggcca gcctcttcga accggatgtc ccggaggttg tttccggaga agacgtgact
     2941 caggctgaac agccgcaaca gccggtgtct cctgccatca acgataagaa gtcagatgca
     3001 ggtgtgaatg ttccggcagg ggggagcgag caggagctga aaatgaaacc ggaagaagag
     3061 atggaacagc aactgccacc cgggatcagt gaatccggtg aagtggtgga tatggccgtt
     3121 tatgaggcat ggcaacggga gcagaatccg gatatccagc agaagatgca gcgtcgtgaa
     3181 gagatgaaca tcaatgtgca ccgggagcgc ggggaggatg ttgagccggg agatgatttc
     3241 tgatgaacag gtcagctcca gtcttcaatt ctcagaccgt ccacacgttc aaattcccgg
     3301 gtgttattag tcacaataat cagtccccga ctgcgggcat gacctgcgat catttgatca
     3361 aatggcccga cagggcgtcc ctgacgggca agttctgctc ttatctggcc gctgtgtgtg
     3421 gctgcggcag cgtcgtaatc cagaacatca atgcgggaaa caaatccctc gatcacagcg
     3481 agattacgtt caggcatctg gcttttttct gcaccatata tcagctccat cagggtgacc
     3541 gaactgatac acattctccc ctggttcagg ttaaaacgct ccctgacgct ggcgggtttg
     3601 ttctttatcg tgaaaatgca gatgttggta tcgagcataa acttcagcat cagaatgact
     3661 ccctttcctg catgccgggc tgttccctgt tatccataaa atcggcgctg acgttgtgtc
     3721 cgtcgaacca ttcgtcccat gtctctccgg ctggcgtaat gattctggtt cgtccgacag
     3781 caatcacttc aacgcgtttt acgttttccg gcaatgcaac cgcttttggc agtctgaccg
     3841 cctggctgcg gttgctgaaa aatacggtgg tttccattat ttgcctcctt atgtggatat
     3901 gtcttgtgta tatccattgt gggattgata ccgggatatg tcaaagggat atacgtttat
     3961 gatgagtatc gcgcaggtca gatcggccgg aagtgccggt aactattata ccgacaagga
     4021 taattactat gtgctgggca gcatgggaga acgctgggcc ggcagggggg ctgaacagct
     4081 gggactgcag ggcagtgtcg ataaggatgt ttttacccgc cttctggagg gaaggctgcc
     4141 ggacggagcg gatctaagcc gcatgcagga tggcagtaat aagcatcgtc ccggctacga
     4201 cctgaccttc tccgccccca aaagtgtctc catgatggcc atgttaggtg gcgataagcg
     4261 cctgattgat gcacataacc aggccgtgga ctttgctgtt cgtcaggtgg aggcgctggc
     4321 ctccacacgg gttatgacgg acggacagtc agaaacggtg ctgaccggta atctggtgat
     4381 ggcactgttt aaccacgaca ccagtcgcga tcaggaacca cagctacaca cgcatacggt
     4441 ggtggctaat gtcacgcagc ataacggcga atggaagaca ctgagcagtg acaaagtggg
     4501 gaaaacgggg ttcattgaga atgtgtacgc taatcagatt gcctttggca ggctctaccg
     4561 ggaaaaactg aaagagcagg ttgaggcgct gggctatgaa accgaagtgg tgggtaagca
     4621 cggtatgtgg gagatgccgg gcgtaccggt ggaggccttt tccggacgct cacaggctat
     4681 ccgggaggcc gtcggggaag atgcctcgct gaaatcccgg gatgtggcgg cactggatac
     4741 gcgaaaatcc aaacagcatg tcgatccgga aatcaaaatg gctgaatgga tgcagaggct
     4801 gaaggaaacc gggttcgaca tccgggcata tcgtgacgcg gcggatcagc gtgcggagac
     4861 ccgcacgcag gcgcccgggg ctgtttcaca ggaggggccg gatgtgcagc aggcggtgac
     4921 acaggcgatt gccggattaa gtgaacgaaa agtgcagttt acgtacacgg acgtgctggc
     4981 cagaacggtc ggtatactgc cgccggaaaa tggtgtgatt gaacgggcac gcaccggtat
     5041 cgatgaggcc atcagtcgtg agcagcttat ccccctcgat cgtgagaagg ggctgttcac
     5101 gtccgggatt catgtgctca atgagctgtc agtccgggca ctcagtcgtg acatcatgaa
     5161 acagaaccgg gtgaccgtac atccggagaa aagtgtcccc cggacggccg gttacagcga
     5221 tgcggtcagt gttctggcac aggatcgccc gtcgctggct attgtgtccg ggcagggcgg
     5281 tgcagccggg ctgcgtgagc gggtggcgga gctggtcatg atggcccggg agcaggggcg
     5341 ggaggtgcag attatcgccg ctgaccgtcg ctcgcagatg aacctgaagc aggatgaacg
     5401 gctgtccggt gagctgataa ccggacgtcg tcagctgcag gagggcatgg ccttcacgcc
     5461 gggcaatact gttatcgttg accagggcga aaaactctcc ctgaaagaga cgttaaccct
     5521 gctggacggt gccgcacgtc ataacgtaca ggtcctgata accgacagcg ggcagcgaac
     5581 cggtacaggc agtgcactga tggccatgaa agatgccggg gtgaacacat atcgctggca
     5641 ggggggagaa cagcggccgg ccaccatcat cagtgaaccg gaccgtaatg tccgctatgc
     5701 ccggctggca ggagattttg ctgccagcgt gaaagccgga gaagagagcg tggcacaggt
     5761 cagcggggta cgggaacagg ccatactgac acaggccatt cgcagtgagc tgaaaacaca
     5821 gggcgtgctc ggacgcccgg aggtaaccat gaccgccctg tcgccggtct ggctggacag
     5881 ccggagccgt tatctgcggg atatgtaccg tccggggatg gtgatggagc agtggaaccc
     5941 ggagacacgc agtcatgacc gctatgttat cgaccgggtg acggcgcaga gtaacagcct
     6001 gaccctgcgg gatgcgcagg gtgaaacgca ggtggtgcgt atttcctccc tggacagcag
     6061 ctggtcgctg ttccggccgg aaaaaatgcc ggtggcagac ggcgagcgac tgagggtgac
     6121 agggaaaatt cccggactcc gcgtctccgg cggtgaccgc ctgcaggtgg catccgtcag
     6181 tgaagatgcg atgacggttg ttgtgccggg gcgggctgaa ccggcctccc tgcctgtgag
     6241 cgattcacca ttcatggcac tgaagctgga gaacggctgg gtggaaacgc ccgggcattc
     6301 cgtcagcgac agtgcgaagg tttttgcctc cgtcacacag atggcaatgg acaacgccac
     6361 cctgaacggt ctggcccgca gcggtcgtga tgtccggctg tattcctcac tggatgaaac
     6421 ccgtactgcg gaaaaacttg cccgccatcc ctcctttacg gtggtttctg agcagataaa
     6481 ggcgcgtgcc ggtgagacat tgctggaaac cgctatcagt ctgcagaaaa ccgggcttca
     6541 cacgccggca cagcaggcta ttcatctggc gcttcctgtg gtggaaagta aaaacctggc
     6601 cttcagcatg gtggacctgc tgacagaggc gaagtcgttt gctgcagaag gaaccagttt
     6661 tactgaactg ggaggggaaa tcaatgcgca gataaaacgc ggtgatttac tgtatgtgga
     6721 tgtggcaaaa ggctatggca caggcctgct ggtttcccgt gcgtcgtatg aggcagaaaa
     6781 gagcattctt cgccatattc tcgaaggtaa ggaggcggtc acgccactga tggagagagt
     6841 acctggcgaa ctcatggaga aactgacatc aggacagcgt gccgctaccc gcatgatact
     6901 ggaaacgtcc gaccgtttca cggtggtgca gggctatgcc ggtgtgggta agaccacaca
     6961 gttccgggcg gtgatgtcag ccgtgaacat gctgccggag agtgaacgtc cccgtgtcgt
     7021 ggggctgggg cccacgcacc gtgcggtcgg tgagatgcgc agcgccggcg tggatgcaca
     7081 gacgctggcg tcctttctgc atgacacgca gctgcagcag cgcagcggag aaacgccgga
     7141 tttcagcaac acgctgttcc tgctcgatga gagctcaatg gtgggcaata ccgacatggc
     7201 acgggcatac gccctgattg cggccggtgg cggtcgtgcc gtggccagtg gtgacacgga
     7261 ccagctgcag gccatcgcgc ccggtcagcc tttccgtctc cagcagacgc gcagtgctgc
     7321 cgatgtggtc atcatgaagg agattgtgcg tcagacgccg gaactgcggg aggcggtata
     7381 cagcctgatt aaccgggatg tggaaagggc actgtccggg cttgagagtg tgaaaccgtc
     7441 tcaggttccc cgtcaggagg gcgcatgggc accggagcac tccgtgacgg agttcagtca
     7501 cagccaggaa gcgaaactgg cagaagcgca gcagaaggcg atgctgaaag gtgagacttt
     7561 tccggatgtc cccatgacac tgtatgaagc cattgtccgc gactataccg gcaggacacc
     7621 ggaagcacgg gagcagacgc tgattgtcac gcacctgaat gaggaccggc gcgtactgaa
     7681 cagcatgatt catgatgcac gggaaaaggc cggtgagctg gggaaagagc aggtcatggt
     7741 gcctgtcctg aacacagcga atatacgtga cggggagctg cgtcgtctct ccacctggga
     7801 gaataacccg gatgcccttg ccctggtgga tagtgtgtat caccggattg ccggtatcag
     7861 caaggatgac gggctgataa ccctggagga tgcggaaggt aacacgcggc tgatttcgcc
     7921 ccgggaggcg gtggctgaag gtgtcacact gtacaccccg gacaaaatcc gggtggggac
     7981 cggcgaccgg atgcgcttca cgaagagtga ccgggagcgc ggttatgtgg ccaacagcgt
     8041 ctggacggtg acagcggttt ccggtgacag tgtcacgctg tcagacggac agcagacccg
     8101 ggtgattcgc cccggccagg agcgggcaga gcaacatatt gacctggctt atgccatcac
     8161 cgcccacggt gcgcaggggg cgagtgaaac ctttgccatc gcgcttgaag gcacggaagg
     8221 taaccggaaa ctgatggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca
     8281 gcatgtgcag gtgtacaccg ataaccgtca gggctggacg gatgccatta acaatgccgt
     8341 acagaaagga actgcccacg atgtgctgga gccgaaaccg gaccgggagg tcatgaatgc
     8401 gcagcggctg ttcagtacgg cacgggaact gcgggacgtg gcggcaggcc gtgccgttct
     8461 ccgtcaggca gggctggccg ggggagacag ccctgcacgg tttattgcac cgggacgtaa
     8521 atatccgcag ccgtatgtgg cactgccggc gtttgaccgt aacggcaagt cagctggtat
     8581 ctggctgaac ccactgacca cggatgacgg aaacgggctg cggggattca gtggtgaagg
     8641 acgggtgaaa ggcagcgggg atgcgcagtt cgtggccctg cagggcagcc gtaacggaga
     8701 gagcctgctg gctgataata tgcaggatgg cgtccggatt gcccgtgata atcctgacag
     8761 tggtgtggtg gtaagaatcg ccggtgaagg tcgcccgtgg aatcccggtg ccatcaccgg
     8821 tggccgcgtg tggggggata tcccggacaa cagcgtccag ccgggtgccg gaaatggcga
     8881 accggtcacg gcagaggtac tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac
     8941 ggaacgccgc gcagatgaaa ttgtcaggaa aatggcagag aacaaacctg acctgccgga
     9001 cggcaaaaca gagctggctg tcagggatat tgccggacag gagcgtgacc ggtctgccat
     9061 atctgaacgg gaaaccgcac tgccggagag tgtgctgcgt gaatcacaac gggagcggga
     9121 agcggtccgg gaggttgccc gggaaaatct gctgcaggag cgtctgcagc agatggagcg
     9181 ggatatggtt cgtgacctgc agaaagagaa aaccctgggc ggagactgat acaggaagac
     9241 aaacactgat gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga
     9301 ccgatacctg gttgcagtcg tttctggtct ggtcacccgg acagcgggat atcatcaaaa
     9361 cggtggcgct ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg
     9421 aatggatgtt tctggccggg cgaggagcct ttccgctgtt tgccctggtg tgggggctga
     9481 atctgtcccg tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa
     9541 ttattgccca gttcgcatat tatctggccg gctttccctg gtatgagggg aatatcctgt
     9601 ttgcctttgc agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta
     9661 cagcagcggc cattcttctg atggccctgt gggggccttt gtccggcacc agttacggca
     9721 ttgccgggct gctgatgctg gcggtcagtt accgactgta ccgggcggaa gacagagcgg
     9781 aacgtctggc gctgcttgca tgcctgctgg ccgttattcc ggcacttaac cttgcctcca
     9841 gtgatgcagc ggcggtggcc ggtcttgtaa tgacggtgct gaccgttggt ctggtgtcgt
     9901 gtgcaggaaa atcattaccc cgtttctggc ccggggattt tttcccggta ttctacgcct
     9961 gtcatctggc tgtgctgggc gttctggcgt tgtgactggt atgatactga cgctgattct
    10021 gattattaat aaacaacata acacaggata tttatgaaaa ttacggatca caaactctct
    10081 gaaggtattg ccctgacatt tcgtgttcct gaaggaaata taaaacatcc tttgattatc
    10141 ttatgccatg ggttttgcgg tatcaggaat gttctgctcc cttgctttgc gaatgccttc
    10201 acagaagcag gattcgcaac gattactttt gattatcgag gattcggtga gagtgatgga
    10261 gaacgagggc gtctggttcc tgcaatgcag accgaagata ttatttcagt tatcaattgg
    10321 gcagaaaagc aggaatgtat tgataatcag cgtattgggt tatggggaac atcgctggga
    10381 ggggggcatg tcttcagtgc agctgcgcag gaccaacgcg taaaatgtat tgtcagccag
    10441 ctggcttttg ctgacggtga tgttctggtt accggagaga tgaatgaatc ggagagagcg
    10501 tcttttctgt ccacgctgaa taaaatggca gagaagaaga aaaacaccgg gaaagagatg
    10561 tttgtggggg ttacccgggt actgagtgat aatgaatcaa aggtattctt cgagaaggta
    10621 aaaggtcagt atcctgaaat ggatataaaa atacctttcc ttacggttat ggagacgttg
    10681 caatataaac cggcagagag cgcagccaaa gtacagtgcc cagttttgat tgttattgcc
    10741 ggccaggata gtgttaatcc accagaacag ggaaaagcct tatatgatgc tgttgcttct
    10801 ggtactaagg aattatacga ggaagctgac gcatgccatt atgatattta tgaaggcgcg
    10861 ttttttgaac gtgtggcggc agtacagacg caatggttta aaaaacatct ttaattgtcg
    10921 gtattaatat gtcagaggtc gttgaggtca gcttaatatc atagtgagta gtttcaacga
    10981 cctggaggac atattttcac aaaggtcagg gcaatcatga cagagcagaa acgaccggta
    11041 ctgacactga aacggaaaac ggaaggggaa acaccgaccc ggagccggaa aaccatcatc
    11101 aatgtcacca cgccaccaaa atggaaggtg aaaaagcaga aactggcgga gaaggctgcc
    11161 cgggaagcag agctgacagc aaaaaaagcg caggccagac aggcgctgtc catttatctg
    11221 aacctgccct cgctggatga ggccgtgaac accctgaaac cctggtggcc gggattattt
    11281 gacggtgaca caccccgact tctggcctgc ggtatccggg acgtgttact ggaagacgtg
    11341 gcgcagcgga atatcccgct ctcgcataaa aaactgcgcc gggcgctgaa ggccatcacc
    11401 cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga cacggaaggg
    11461 tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cggcagggcg tttggagaaa
    11521 attcgccgcc agaaccggac aaaggcagaa cttcaggccg tgcttgatgg gaaataaaaa
    11581 aagcctcccc ggaaaccggg gaggctgaaa caataaggct taaaaggaaa taacttccgt
    11641 acccgaaagg aagccagtcc actgaattat ttaccacaat aatactgacg actaaaaggg
    11701 aggaacagaa aaatgaatgg attcagaaac agttcacgga acggtcaggt ctggcgttac
    11761 cagcgtgccg gcgggcgggc cgttattctg gaggtcagtg gacgctggat ggaagcggca
    11821 gaagcatggc gacgggctgc ctgcatagct ccccggacag actggcaaca gtttgcccgg
    11881 aaaagggctg aacactgcca ccggcgctgc cggggaaggg tgtaacgaaa aaagcagcct
    11941 tcattactcc aggagtcaga aggctgaaag gagtttatga gatttgcttt ttttaatatc
    12001 cggtcgggca gtatgaccgg ccagttctga gtagcacaga gttttttccg tattaagtcg
    12061 cgattttata ttttgtgatt aatttcacaa aataaggtct tattcaatgt gtgctgcgat
    12121 attcaggggg gctgaaatac aggtgccatt tatctgatat ggagaataac atgaggaaat
    12181 atattccact ggtattattt atcttttcat ggccggtatt gtctgcagat gttcacgggc
    12241 agatttatgc accagacaga atgaatgtta attagtttat gaatacaaca ggggctgatc
    12301 agcaatcacc ggaacatcgt agctgacgcg ttcgcaatgc tcagttgtcc cccccggaaa
    12361 cgggaaaaag taagttttcc cccgctcccg gcgtttttgt aacaaaaaca ggcaaatggg
    12421 ggtaatagtc agcaacaggg aatgtggtat tatcgcggcg ggtgtttgag cctttctggt
    12481 tcaggcaaga cgcaggtacc agaaatgcga agaccccact cgttaatcca ttaactcgtg
    12541 aggtctgcat gaagtacctt aacactactg attgtagcct cttctttgct gagaggtcaa
    12601 agtttatgac gaaatatgcc cttatcgggt tgctcgccgt gtgcgccacg gtgttgtgtt
    12661 tttcactgat attcagggaa cggttatgtg agctgaatat tcacagagga aatacagtgg
    12721 tgcaggtaac tctggcctac gaagcacgga agtaagctgc cgggcgggga cggaagtccc
    12781 cgctttccgg aagtgtgagg tatttcaggg gcagacaccc tatataccag aaacagccgg
    12841 tcccgcccgg ggccggcacc ctggctaagg catttccagc ttttcagtca tttcattatc
    12901 aaaatcacat taaacggtcg taatcagaca tgatttgtgc gccaacacag atcatcgtca
    12961 caactttcaa gtcgctgatt tcaaaaaact gtagtattct ctgcgaaacg atccctgttt
    13021 gagaattgag gaggcgagat gtcgcagaca gaaaatgcag tgacttcctc atcaggcacc
    13081 aagcgtacat accggaaagg taaccctgtt ccggccagag agaggcaaag agcttctctg
    13141 gctcgcagaa gcaacactca taaggctttt catgcggtta ttcaggcccg gttaaaagac
    13201 aggctgagtg aactggcgga tgaggaaggt attactcagg cgcagatgct tgaaaaactg
    13261 attgaatcag agctgaaacg cagagcgact tcgtaaatat tcacattctt gcttatctca
    13321 ggagtgagtg gtagattgct gatcgtttaa ggaattttgt ggctggccac gccgtaaggt
    13381 ggcagggaac tggttctgat gtggatttac aggagccaga aaagcgaaaa ccccgataat
    13441 cttcttcaag tttggcgact agaaagatta ccggggcccc ataaaaccgc atagccaaca
    13501 attcagctat gcggggagta tagttatatg cccggaaaag ttcaagactt ctttctgtgc
    13561 tcactccttc tgtgcattgt aagtgcagga tggtgtggct aatcatgaaa cacattcagt
    13621 aatagcgggt gggattgaat cagatcttca cattgattcc agcaagtatc ctcacccgtt
    13681 ttgcagcctt ctccagaaaa gggctcattt tgactccttc aagcatctga tcttcatcag
    13741 aggtttgctt gcaatagcgc atggcaaacg taaaaataaa atcagcgcgt cgatggttag
    13801 tttttatgtt tccctcgtac aagtaatgtg cgcacactac atccctgata cgaacaaagt
    13861 taacttatct gttaaagagc tttcagtttg tagtggaggt tcatacactc gtgtttggag
    13921 agccctcaaa actcttgata atgatcttca tctcatcgct tttgatggag gaaccatctg
    13981 gttcagacca gatatgttcg aaactttacg tgtcggccca gacgagctag ttaccgcccg
    14041 taggaggggg aatagtgttg gagggggaca tggctgatct ccttcaaaaa tactattcac
    14101 aggttaaaaa tccgaatccg gtgttcacac cccgtgaagg tgccggaacg ctgaagttct
    14161 gcgaaaaact gatggaaaag gcggtgggct tcacctcccg ttttgatttc gccattcatg
    14221 tggcacatgc ccgttcgaag ggactgcgtc ggcgcatgcc accggtactg cgtcgccggg
    14281 ctattgatgc gctgctgcag gggctgtgtt ttcactatga cccgctggcc aaccgcgtcc
    14341 agtgctccat cactacgctg gccattgagt gcggactggc gacggagtct gctgccggaa
    14401 aactctccat cacccgggcc tcccgagcct tgacgttcct tgcagagctg ggactgatta
    14461 cctaccagac ggaatatgat ccgcttatcg ggtgctacat tccgaccgat atcacgttca
    14521 caccggcgtt atttgccgcc cttgatgtgt ctgaggatgc agtggttgct gcgcgccgca
    14581 gtcgtgttga atgggaaaac agacagcgca aaaagcaggg gctggatacc ctgggtatgg
    14641 atgaactgat agcgaaagcc tggcgttttg tgcgtgagcg tttccgcagt taccagacag
    14701 agcttaagtc ccgtggaata aagcgtgccc gtgcgcgtcg tgatgcgaac agggaacgtc
    14761 aggatatcgt caccctggtg aaacggcagt tgacgcgtga aatctcggaa gggcgcttca
    14821 ctgccaatcg tgaggcggta aaacgcgaag tggagcgtcg tgtgaaagat cgcatgattc
    14881 tgtcacgtaa ccgtaattac agccggctgg ccacagcttc cccctgaaag tgacctcctc
    14941 tgaataatcc ggcccgcacc ggaggcatct gcacgcctga agcctgtcgg cgagcaaaaa
    15001 aacagcaccg catacaaaaa acaacctcat catccacctt caggtgcatc cggtccctcc
    15061 tgtttttgat acaaaacacg cctcacagac gaggaatttt gcttatccac atttaactgc
    15121 aagggacttc cccataaggt tacaaccatt catgtcataa agcgccatcc gccagcctta
    15181 cagggtgcaa tgtatctttt aaacacctgt ttatatcttc tttaaactac ttaactacat
    15241 tcatttaaaa agaaaaccta ttcactgcct gtcctatgga cagacaggta tgcacctccc
    15301 accgcaagcg gcgggccccg accggagcca ctttagttac aacactcaga tacaaccacc
    15361 agaaaaaccc cggtcccgcg cagaactgaa accacaaagc cccccctcat aactgaaaag
    15421 ccgccccgcc ccggccctaa gggccggaac agagtcgctt ttaatcatga atgttgtaac
    15481 tacatcatca tcgctgtcag tcttctcgct ggaagttctc agtacacgct cgtaagcggc
    15541 cctcacggcc cgctaacgcg gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca
    15601 ctccgaccgc gcacagaagc tctctcatgg ctgaaagcgg gtatagtctg gcagggctgg
    15661 ggatgggtaa ggtgaaatct atcaatcagt accggcttac gccgggcttc ggcggtttta
    15721 ctccggtatc atatgcaaca acggagtacc gccttccatg ccgctgacgc ggcatcagaa
    15781 taatacaaac agcatttaat gcatttgcaa aagtgtgagt aataaagact tgttaagtgg
    15841 tcgtttcagg gatatcgtta agacttgtta aatagtctca aaggtgcgtt atgcctaaca
    15901 tcattctgtc tgacaccagt gcaagtgtca gtgaactgaa aaaaaatcct atggctacag
    15961 tcagtgccgg agatggttac cctgtcgcca ttcttaaccg gaatcagccg gcattctact
    16021 gtgtgcctgc tgaactgtat gaaaggatgc ttgatgctct ggatgaccag gaactggtga
    16081 aactggttac tgaacgcagc aatcagccac tgcatgatgt ggacctggac agttatttat
    16141 gacttatacg gtaaaattca gggatgatgc gctcaaggaa tgggtgaaac tggataagac
    16201 cattcaacaa cagtttgtga aaaaactaaa aaaatgcagc gaaaatcctc atataccgtc
    16261 ggcaaaacta agagggctaa aggactgcta caaaattaaa ctacgtgcat cgggatttcg
    16321 tctggtttat caggtgattg acgatatgtt aattattgcg gttgttgcgg taggaaaacg
    16381 agaacgtagt aacgtttata atctcgccag tgagagaatg agatagtaac atataaatag
    16441 cctttcccgc agataactct cagaattgtt attttctctg tggactcgcc tgaggcaggg
    16501 gaagaaaagt tttgttgaga gtatgatttt acctgccggg atgagtcccg ggggatttat
    16561 atcatcagac aaactggaac ggagagacat gttttatgac aatactcgtg acggcacaga
    16621 ctcaactcat agttggtgag gctatggcga taaaaagttt agctcctgat ggaatgttgg
    16681 ccgctgtatt tgaagatgac gggaataccg gatatttcta tgcacttgat gagtccgctg
    16741 aaggtaaccc tgtcagggat gctgtgcata tatataacgt tgaagatatt tcagatgctc
    16801 acatcccctc tgatgtgaag attggctggt ctgaggactg cctgaagtgt gttctgctga
    16861 taaacggata tccacatggt gtatttgatt ttgaggggaa gaacggctac tgtcgaagtg
    16921 gattcccgcc tccggttaat cgtgagtggt ctgttcaggg gcatgcatgg gatgattacg
    16981 ttgataattt cttccggtga ttgaaccgaa aaattattag ctggagcggt gccggattaa
    17041 gtgcaggagc cggctttgtt ggcggcatga cagacaatgt attttttggt aatactgtaa
    17101 aaggagctat cgcaagcact gttttaagtt cttcagcatc aggagcaata aaagctacag
    17161 gtaaaaacgg ccaatcatcc tgtcattaaa ggagcattta taatgctgaa gttgctgaat
    17221 gggacaagtt acggcaacga ttgcttgatg ccagaaataa aatcacctct gctgaatctg
    17281 cggtaaattc ggcgagaaat aacctcagtg ccagaacaaa tgaacaaaag catgtaaatg
    17341 acgctcttaa tgccctgttg aaggaaaaag agaatatccg taaccagctt gccggcatca
    17401 atcagaagat agcggaagag aaaagaaaac aggatgaact gaaggcaacg aaagacgcaa
    17461 ttaatttcac aacagagttc ctgaaatcag tttcagaaaa atatggtgca aaagctgagc
    17521 agttagccag agatatggcc gggcaggcta aagggaagaa aatacgtaat gttgaagagg
    17581 cattaaaagc atatgaaaag taccaggctg acattaacaa aaaaattaat gcaaaagatc
    17641 gtgcagcgat tgctgccgcc cttgagtctg tgaaactgtc tgatatatcg tctgatctga
    17701 acagattcag tcggggaccg ggatatgcag gaaaatttac aaatcttgct gactggatca
    17761 ctgagtttgg taaggctgtc cggacagaga actggcgtcc tctttttgtt aaaacagaag
    17821 ccattatagc aggcaatgcc gcaacggctc ttgttgcact ggtcttcagt attcttaccg
    17881 gaagcgcttt aggcattatc gggtatggtt tactgatggc tgtcaccggt gcgctgattg
    17941 atgaatcgct tgtggaaaaa gcgaataagt tctggggtat ttaaataaaa tataagacag
    18001 gctgtctatc ttacagacag cctttttata ataacagaaa gaatatatat cagacttaac
    18061 ggaagagata atatgaaaac agtacccgta taaacagcaa gcaaacccat ttttccaggt
    18121 gtatcagcaa aaaaaccgct gttccagaaa tgaagtgggg taaatttcag agctgtatct
    18181 tcaatatacc attttgcaac cggatacaaa accattccgc agagagaaat acaccagaac
    18241 agtaatctgt atttaaaatc ataatcccat gacatataca gcatatatcc ccccgtcacc
    18301 catccccacc acatattatt aaaataatat tttctgttca tctgcctgtt ttcctttctt
    18361 ttgcactttt gtcagtgtac tgatgcatga caacataacc acagcatgat aataataatc
    18421 aataataata agctgtgtca cgtttacatc atggaacgga ggtgctcctg tgacttttga
    18481 gcagaaaaaa gcccgggcca ttgccctgat ggacagcaaa aagatgtggc gaagcaatta
    18541 tgcccctcct cttctgcgta ttctatggcg gttgggtatc cggcttccgc cattaccctt
    18601 tatgccgttc tggcaggtca cgctgttgat ggggagctta tggggcattt cctggggatg
    18661 tgcaatgtgg tttatctacc gggggccgtc cggaatggtg gcaggtgagg ccattattat
    18721 aagtatcacg ggtggttttt tgtttggact gctcatggcc tctttccact ggtggcgcag
    18781 gaaagttaac cggctgccat catgggatga tgtgtaaacc gtgcacaggc gtgttcttac
    18841 ggagcggcac tttcatttat ctgtacgcga acctccattc ctgcatcgtg acaggcctgc
    18901 agccactgcg ccacttccag cggatcgccc tcccggcgta ccactctgcc ttctttattc
    18961 cataactgca gacaggtgct gccgtcgaga cgcaccacaa aatccccacg gcaggcctga
    19021 tacgttatgc cgttcagctg caccgccaga tcagtggcag aagtgccgtc tgcgggcaga
    19081 cctgttgccg ggaccagcgg cagtacgacg acttctgccg cacgctgttc tgcccgga
//
LOCUS       DAFAVF010000077        18469 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFAVF010000077 DAFAVF010000000
VERSION     DAFAVF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.077"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            427..1033
                     /locus_tag="LGK42_002681"
                     /pseudo
     CDS             427..1033
                     /locus_tag="LGK42_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1136..1639
                     /locus_tag="LGK42_002682"
     CDS             1136..1639
                     /locus_tag="LGK42_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBK2658912.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1706..3163)
                     /gene="nuoN"
                     /locus_tag="LGK42_002683"
     CDS             complement(1706..3163)
                     /gene="nuoN"
                     /locus_tag="LGK42_002683"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBK2658913.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3170..4699)
                     /gene="nuoM"
                     /locus_tag="LGK42_002684"
     CDS             complement(3170..4699)
                     /gene="nuoM"
                     /locus_tag="LGK42_002684"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBK2658914.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4863..6704)
                     /gene="nuoL"
                     /locus_tag="LGK42_002685"
     CDS             complement(4863..6704)
                     /gene="nuoL"
                     /locus_tag="LGK42_002685"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBK2658915.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6701..7003)
                     /gene="nuoK"
                     /locus_tag="LGK42_002686"
     CDS             complement(6701..7003)
                     /gene="nuoK"
                     /locus_tag="LGK42_002686"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBK2658916.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7000..7554)
                     /gene="nuoJ"
                     /locus_tag="LGK42_002687"
     CDS             complement(7000..7554)
                     /gene="nuoJ"
                     /locus_tag="LGK42_002687"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBK2658917.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7566..8108)
                     /gene="nuoI"
                     /locus_tag="LGK42_002688"
     CDS             complement(7566..8108)
                     /gene="nuoI"
                     /locus_tag="LGK42_002688"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBK2658918.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8123..9100)
                     /gene="nuoH"
                     /locus_tag="LGK42_002689"
     CDS             complement(8123..9100)
                     /gene="nuoH"
                     /locus_tag="LGK42_002689"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBK2658919.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9097..11823)
                     /gene="nuoG"
                     /locus_tag="LGK42_002690"
     CDS             complement(9097..11823)
                     /gene="nuoG"
                     /locus_tag="LGK42_002690"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBK2658920.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11876..13213)
                     /gene="nuoF"
                     /locus_tag="LGK42_002691"
     CDS             complement(11876..13213)
                     /gene="nuoF"
                     /locus_tag="LGK42_002691"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBK2658921.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13210..13710)
                     /gene="nuoE"
                     /locus_tag="LGK42_002692"
     CDS             complement(13210..13710)
                     /gene="nuoE"
                     /locus_tag="LGK42_002692"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBK2658922.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13713..15515)
                     /gene="nuoC"
                     /locus_tag="LGK42_002693"
     CDS             complement(13713..15515)
                     /gene="nuoC"
                     /locus_tag="LGK42_002693"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBK2658923.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15609..16271)
                     /gene="nuoB"
                     /locus_tag="LGK42_002694"
     CDS             complement(15609..16271)
                     /gene="nuoB"
                     /locus_tag="LGK42_002694"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBK2658924.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16287..16730)
                     /gene="nuoA"
                     /locus_tag="LGK42_002695"
     CDS             complement(16287..16730)
                     /gene="nuoA"
                     /locus_tag="LGK42_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBK2658925.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17361..18299)
                     /gene="lrhA"
                     /locus_tag="LGK42_002696"
     CDS             complement(17361..18299)
                     /gene="lrhA"
                     /locus_tag="LGK42_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBK2658926.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4623 a         5125 c         4729 g         3992 t
ORIGIN      
        1 caaaagagtg agttaacatc attgttaatt aattcgacca gtcagcaaat atgctgactg
       61 gtcgaacata gtttgctaaa cagtcatcac tgactgatgt cagtcacacc atccgccagt
      121 tcaatcttcg ggtatttcca gcgtatctcg gcacgcgatt gctggtctgt tgatgtagtg
      181 gattgttgtt gctgactttt cttaccctca gagttctgcc ccctgtcaac tcaatctgcc
      241 aataaataac ataattatat ttttccttat atgaattcct ttttaaaata gcgaagccac
      301 tgtaaaagat actttttgta tagatctaac aaccgtaata aaacaaacac tcataactca
      361 ttataaataa tgataaaata acgtctttta ttgataattt atatatgaaa aaaatccttt
      421 tgttatatta tttctgattt cactccctct gattattttt accaaccctg ggtcactgca
      481 ttatcgccaa cgccccgaca cgtcagtcct gagcaattag aaaaaacagt tcgttatctt
      541 acacaaactg tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag
      601 tatataaaag agttctttgt cagtagcggt gccagagtta ccgcgcaggg cgtccccgat
      661 tggctattac gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt
      721 tatcccgatc ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc
      781 gtcaggtaaa aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc
      841 catggtttat tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc
      901 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
      961 atacagcaga cagattgaat tatcagaaaa tggctcagat ggcgttataa ccttgttaca
     1021 caacagtaaa taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta
     1081 ttttccctcg tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg
     1141 aatgattggt tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac
     1201 tgaaaaacct ttgatggata atatacatga cacgctttca ggattacgcc gactggatat
     1261 tgataaacgc tgggattttt tacactttgg gctgacaggc acctctgctt ttgatcccgc
     1321 gaagaatgat ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga
     1381 tggcttcctg ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc
     1441 actggatcga agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat
     1501 ttatccggga gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct
     1561 tgatatggaa aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct
     1621 gacggttatt gttgggtgat ggtgtcaggg caccaaaaaa aaccgccgaa catgtcggcg
     1681 gctttctgac ttacaaactg atagcttaca tcagcggcat tgctaaccgc acaatgctaa
     1741 tcagcggttg tggccataca cccagcacca gtaccaacag tgcagagatc agcaccacga
     1801 taccgcctgc gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggggcgt
     1861 gcaggtacag gctcaccgcc acgcgcaggt agtagtaaag gccgattgcc gaaccgacaa
     1921 ccacggcgcc caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact
     1981 taccaataaa gcccagcgtc atcggaatac cggccagaga cagcatcatc accgtcatca
     2041 ccgccgcgag gatcgggcga tgccagaaca gaccgcggta agagaacagg gaatcagcat
     2101 ccgggccacg gtacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga
     2161 acagataacc ggccaggtaa actcctaccg cttccatcga catctcgccc gtttgcagcg
     2221 caatcagcgc caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt
     2281 tgatattggt ctggctcagc gccatcaggt taccgaagat gatagaggca aaggcgataa
     2341 tcgccagcac tacccgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca
     2401 tcaccacacc gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag
     2461 gcgcgccctg gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc
     2521 cgaggccaac aatcatcagt ccgaaacctg ccagcaacag cggctcgttg agcataccgt
     2581 cgccgaggtt tttacccagc gcgacaaacg acaggtcgcc agactgtgca tacaccagcg
     2641 ccataccaaa cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg
     2701 cttccagcga acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga
     2761 tcagttcaat accgaggaac aaagacgcca gatggttggc attcgccagc aggatcccgc
     2821 ccagcgcggc aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa
     2881 gccacgggta ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa
     2941 gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccga
     3001 caaaccagag cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga
     3061 aatgattgcg tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca
     3121 gcaacggtag cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg
     3181 tagtagtaac ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat
     3241 ccagaatcgg ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca
     3301 taaacagctc acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt
     3361 taccgaagta agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa
     3421 aggtagagat aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt
     3481 cgccgacgaa gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca
     3541 gtgctggcag ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat
     3601 ggatacgttc ataaagctga ccacaaagaa taaacagacc cgccgccgac aagccgtgcg
     3661 caatcatctg gattaccgcg ccctggtagg ccaactggct gccggtgtag atagcaatca
     3721 gcacgaagcc catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctggg
     3781 cgaaagccat ccacgcaccg tagaagatgc cgataacccc cagccacata gcgattggcg
     3841 caaactctgc cgatgcgttc gggaacagcg gcagggagaa acgcagcaaa ccgtaagcgg
     3901 cagttttcag caagatcccc gcgaggtcaa cggaaccggc ggtcggagcc tgagagtgcg
     3961 catccggtag ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga
     4021 agcccagcat taacaggtat tccacaccac tggacattgg cgtattcagc agctcttcat
     4081 agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcaa
     4141 tcaacatcac caggccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac
     4201 gcgttttacc gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca
     4261 gcatcatttc ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa
     4321 cgccgcccag gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt
     4381 tccacgaaca tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg
     4441 acagcccgtc aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga
     4501 attcagactg ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc
     4561 acagttgcag cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca
     4621 ccttgacgcc aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata
     4681 ttagccaggg tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat
     4741 tttcaacgaa ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcacc
     4801 gcgaaggcgg cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta
     4861 actcaacgca gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc
     4921 acataccagc gcagatagcc gttctcactt aacagcagac ctttacctgc aaagcgggaa
     4981 agaaccgccg ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata
     5041 cccaggaacg gcttgacgaa cactttgtcg tacagccagt caaagcccca ggcgttgtac
     5101 caccaggtac tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagtacgt
     5161 ttacccagcc acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta
     5221 atttccaggg tcaacatgct gccatgcgcc agttccgtcg tttgcggaag cacgccctgc
     5281 agcggcggta caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc
     5341 aggctgtgag ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg
     5401 atgaaaatca tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc
     5461 atcagattga tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag
     5521 cccgcagtga ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa
     5581 accagcggaa tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag
     5641 gccagaatga cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc
     5701 aagtggaaaa tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc
     5761 tggctcatgg tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa
     5821 ccggccagca gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc
     5881 atcaggaaca ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg
     5941 gcgtggatca gcgcggagac aggcgtcggg ccagccatcg catcggcaag ccatgtctgc
     6001 aacggcaact gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc
     6061 agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc
     6121 agggtgccca gttcgttgta aagaatgaac agtgcgaaag cgaggaacac gtcacccaca
     6181 cgggtcacga cgaacgcttt cattgccgct gcgccattct tcggatcggt gtaatagaaa
     6241 ccgatcagca gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg
     6301 ttgtcggcaa gcaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag
     6361 cgagagtagc cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc
     6421 acaccagtga ccaccgagag catggtcagc gacaggccgt ccagcaccag attgaaaccg
     6481 atgttaaagt cgcctaccga catccacgtc cacagcggct ggctgtatga ctgctcgccg
     6541 ttagcgaaga aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca
     6601 acgccgacga tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg
     6661 aagccaatca atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta
     6721 ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga
     6781 tactcgcttc tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct
     6841 gaccccagta gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca
     6901 gaccaatcag cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga
     6961 ataagattgc cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt
     7021 tcttttcgcg ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc
     7081 gacgtggaag gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag
     7141 tacgtaaggc ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat
     7201 accctgatca ttaacaccaa ggatggcgta aacaatcacc accagcatga tggccgacaa
     7261 aattgccgga ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga
     7321 accgcccagg ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta
     7381 gacgataatt tccagcgcac cggcgaagta agcgcccagt gagaagaaca ccccggaaat
     7441 cgccagcagc gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac
     7501 tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc
     7561 tctccttacg gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc
     7621 ttatctttgc cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg
     7681 cccggaccgg agatcagcag atcctctttt tcgtaaacca gatcctggcg cttgtattcc
     7741 cccatttcga aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga
     7801 ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt ccgggtacca gcgaccgtct
     7861 ttggtttctg ctttttgcag cgagatacag ccgaccgggc aggctaccgc gcagaggtta
     7921 caggctacgc aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa
     7981 cggggcggca gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg
     8041 tgcaggccga tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt
     8101 aaggtcatgg tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca
     8161 gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acctggtcat
     8221 aacgcggacg cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt
     8281 tcagcgcgaa ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg
     8341 tcaccatcaa tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga
     8401 acttcatacc ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct
     8461 ggtcaaacgg gtgacggtga cataccgcca cgcccgcgat ggcaaaggta ataaaaccaa
     8521 agaattgcgg gataacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga
     8581 atgaaccggc ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca
     8641 gggtctgcgc agaagcacgc atcgcaccca gcaacgagta tttgttgtta cttgaccagc
     8701 ccgcaaacag caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga
     8761 tgttcaggtc ggcaaccacc caacccggac tgactggcac aatcgcaaag gccagcagca
     8821 gcgaggtaaa ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga
     8881 tccagtcttc tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc
     8941 agccaacacg gttaggtccg taacggttct ggaacagacc cagcaggcga cgttcgccaa
     9001 agctcatgaa tgccccgcag gtgacaacca ccagcaggat caccaccgct ttgaggatgg
     9061 tcagcaggat ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc
     9121 ctcaagatgc gcgccagcca gcaccggagc aatgccggac atacccatcg gcaaacccac
     9181 ctgccctgcc gtcagccctt cagcgatttc aaccggcagc gtgaccgtgt tgccatcgta
     9241 actaaaggag acgcgagcgc ctgcgttcac gcccaacttc gcggcatccg ctgggttgag
     9301 tttgatgtac ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc
     9361 gctgccaaac aggtggtaat acggcgcgat acgccatttc ccgtcctgcg gctggaagcg
     9421 tgccggtacg ctggtgaagt aatccagacc attttcgctg gtttcaaaca gacgcacgcc
     9481 cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg
     9541 ggagttccag cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcag tcggctggtt
     9601 gttaccttcc atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg
     9661 aacgctgata ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag
     9721 tttctgccca cgaatacgga atgtcgcatc cggcgcagca tctttgatac ctgccagttc
     9781 cgggattttc gccacaacag cgtcaatcac atggtcgagc tgcgtccagt ccacttcacg
     9841 gctcagcagg gtgctgtgca gcgagtgtaa ccagcgccag ctttccagca tgacagtttt
     9901 gctgtcgtaa taagcaggat cgtaaacctg gaagaaacgt tgggcgcggc cttcgttgtt
     9961 gatcaccgta ccgtcgcttt cagcaaagct ggcagcagaa agtaccagat gggcgttttc
    10021 cataatcgct gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc
    10081 attcacgcgg gtagcggaag cgtgacgatg cagatcgttt tccagcacca ccaccgcgtc
    10141 ggcgcgtccg gtttccagtt cggtcaacgc ttcttcaagc gaaccgccac ccataatgcc
    10201 cagccccatg ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc
    10261 tttcagggct ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt
    10321 cccggagata atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt
    10381 gctttgcagc tcaggttcga taccgtcaac cgctggcgca gagttatcca gcgcatgggc
    10441 gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat
    10501 atcatccaga cgggtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc
    10561 gatgttgagg attgccgcaa tctgccagtc agccactttc tgtgctgccg ccatttcgcg
    10621 cgctttacct ttcacagcct gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac
    10681 gtcttcgccc agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata
    10741 aatgccgcct tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc
    10801 gataccggtg tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac
    10861 gctggcacgc ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc
    10921 gccctgcatt gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg
    10981 ctgacgcgga cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag
    11041 gaagtagtgg tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg
    11101 ttcaccgggg ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg
    11161 catatcccat ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg
    11221 gcaaatttcg accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc
    11281 gaagtagacg ttgtcgtgcg caccgtaaac gcccagatct gtaccgtcag cgtaatcttt
    11341 gtagtaacgc acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg
    11401 ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc
    11461 ggtcatcacg gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc
    11521 gtgcgggtgg ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc
    11581 gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg
    11641 accacgcgtg tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac
    11701 gcttcccagc gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc
    11761 ttccagcagg ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc
    11821 cattagcatg cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc
    11881 agcgctcttt cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct
    11941 gtttgattcc cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca
    12001 ctgcaccagg tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct
    12061 caagtgtttc gatatcgccc ggctgacctt caccacgctc cagcgcacgc agaattttca
    12121 cgctccacgg cagaccgtcg cggcacggcg tacaccagcc gcaggattca cgggcgaaaa
    12181 actcttccag gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca
    12241 gcgccgtacc cagacggctt cccgctttac cgatactttc gaattccatc ggcagatcaa
    12301 ggtgcgcttc ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca
    12361 gaccatcacg cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg
    12421 gcagttccca cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag
    12481 catctttact tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa
    12541 cgttacacag ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg
    12601 ccgggaaggg gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg
    12661 ctgtttcttc cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga
    12721 aacctgttcc cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc
    12781 ggcgcagatt tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag
    12841 ctttcagcgc aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca
    12901 tcagcaggcg gtctttatag gtgcctggct ccatttcatc ggcgttacac agcaggtaac
    12961 ggatgttcat ggattcgtct ttcggcatca ggctccattt caggccggta gaaaagcccg
    13021 cgccaccgcg ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca
    13081 gcccggtcag cgccttacgc gcgccttcgt aaccgttttt gctgcgatat tcgtccagcc
    13141 acactggctg tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga
    13201 taatgttttt catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc
    13261 gtgagtatcc tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt
    13321 tggcagcagc gtaaagcgac cgtcaaatgt cgtttgccct ggtttgatgt ttagcttttt
    13381 ctcgagcgcc gcctgaatac cctggtaacc gttgatatga cagaccacgc tgtcgcaata
    13441 acggatcaca tggcggccaa ccggctggcg gaagatctgg ctgtagaacg ttgccacacc
    13501 ttcgacgtcg cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg
    13561 cacccagcca cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc
    13621 ttcgtagtgg tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc
    13681 ctcggtttgt ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa
    13741 tcgatactgc ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga
    13801 atttgctgca aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta
    13861 ccgtcgctgg tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat
    13921 tcattggcag gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg
    13981 atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac
    14041 gggccttccg gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct
    14101 tccactttca gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca
    14161 aagtcgaagt tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg
    14221 gtagcacgca ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg
    14281 gcaacgccct gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac
    14341 gccagacgtt tcggcatcca gtcgaggaac tcacgcagca ggcgatccca gccgcgcggc
    14401 aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc
    14461 gcttccacca gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt
    14521 gcgccgacgt cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt
    14581 tcggagagca taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc
    14641 tctaccgcca gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg
    14701 gtatacggaa tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg
    14761 tggtagccga tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata
    14821 cggaaagccc cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg
    14881 ttttcggtgc cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc
    14941 agatcctgtt tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa
    15001 tctttacgca gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg
    15061 tgaccgtcga aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc
    15121 gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc
    15181 atgatgtcgc ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca
    15241 ggtaaccctt cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg
    15301 acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg
    15361 atccacacaa cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa
    15421 cggttgcgca gttcgccaat caccggatca tcaagatgat cgcgggtctg ccaggcgggt
    15481 tcttgcgcgg ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg
    15541 gcgcagtaag cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca
    15601 ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc
    15661 ttgcgttcgc gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag
    15721 gagagcggac gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac
    15781 gcttcaggac gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg
    15841 ccctgcacaa cggaataaat atcgtacata ccgccggagt tggcacaggc acccattgag
    15901 ataacccatt ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg
    15961 gtaaagcagg ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact
    16021 tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa
    16081 cagcaggaaa gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg
    16141 tcattgagct tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg
    16201 atctcctgct tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc
    16261 gtataatcca tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc
    16321 gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc
    16381 agataaacca gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc
    16441 cagccgcttt cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag
    16501 ataacgaaga acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg
    16561 accgagtcga taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct
    16621 aaaaaccaac cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata
    16681 gcgaatgccc agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca
    16741 tcaaaagtag cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg
    16801 ggaggaataa aaaaaacctt acaatcactg tagaaattct tttatacagc taattgatgt
    16861 ggtcttttac tcctttctat aaccttttgt caactttaac aaaagtttct tcacattagt
    16921 ttacataata tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc
    16981 caggataatt agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc
    17041 tattttgaca ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt
    17101 gacacgcttt tgtcattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat
    17161 cattgtagca ggtgattttt ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg
    17221 tgattgaaaa atgaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa
    17281 aaaaagccgc tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg
    17341 aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct
    17401 tccggagcag acattgggct gtattgccac ggattatggt agctctccat cgcctgatag
    17461 atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca
    17521 ggtagcggcg gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca
    17581 accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc
    17641 gaggcgacat aagccagtcg ccatggaata tctgctttat taagcgtcgc cagcaccata
    17701 tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat
    17761 acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac
    17821 gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc
    17881 atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg
    17941 cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac
    18001 acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt
    18061 ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga
    18121 cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg
    18181 gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca
    18241 acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata
    18301 tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc
    18361 aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa
    18421 tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatg
//
LOCUS       DAFAVF010000078        18465 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFAVF010000078 DAFAVF010000000
VERSION     DAFAVF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.078"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..780)
                     /gene="umuC"
                     /locus_tag="LGK42_002697"
     CDS             complement(<1..780)
                     /gene="umuC"
                     /locus_tag="LGK42_002697"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HBK2658927.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITD"
     gene            complement(780..1217)
                     /gene="umuD"
                     /locus_tag="LGK42_002698"
     CDS             complement(780..1217)
                     /gene="umuD"
                     /locus_tag="LGK42_002698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBK2658928.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1214..1462)
                     /locus_tag="LGK42_002699"
     CDS             complement(1214..1462)
                     /locus_tag="LGK42_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBK2658929.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            1880..2782
                     /locus_tag="LGK42_002700"
     CDS             1880..2782
                     /locus_tag="LGK42_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBK2658930.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            2779..3090
                     /locus_tag="LGK42_002701"
     CDS             2779..3090
                     /locus_tag="LGK42_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658931.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            3167..3850
                     /locus_tag="LGK42_002702"
     CDS             3167..3850
                     /locus_tag="LGK42_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBK2658932.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            3851..4072
                     /locus_tag="LGK42_002703"
     CDS             3851..4072
                     /locus_tag="LGK42_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658933.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            4084..4518
                     /locus_tag="LGK42_002704"
     CDS             4084..4518
                     /locus_tag="LGK42_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBK2658934.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            4569..5339
                     /locus_tag="LGK42_002705"
     CDS             4569..5339
                     /locus_tag="LGK42_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658935.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            5880..6182
                     /locus_tag="LGK42_002706"
     CDS             5880..6182
                     /locus_tag="LGK42_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2658936.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            6229..6651
                     /locus_tag="LGK42_002707"
     CDS             6229..6651
                     /locus_tag="LGK42_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBK2658937.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            6648..6839
                     /locus_tag="LGK42_002708"
     CDS             6648..6839
                     /locus_tag="LGK42_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658938.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(7172..7240)
                     /locus_tag="LGK42_002709"
                     /pseudo
     CDS             complement(7172..7240)
                     /locus_tag="LGK42_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            7609..8136
                     /locus_tag="LGK42_002710"
     CDS             7609..8136
                     /locus_tag="LGK42_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK2658939.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            8194..8427
                     /locus_tag="LGK42_002711"
     CDS             8194..8427
                     /locus_tag="LGK42_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBK2658940.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            8486..10444
                     /locus_tag="LGK42_002712"
     CDS             8486..10444
                     /locus_tag="LGK42_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK2658941.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKSRL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            10499..10933
                     /gene="psiB"
                     /locus_tag="LGK42_002713"
     CDS             10499..10933
                     /gene="psiB"
                     /locus_tag="LGK42_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBK2658942.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            10930..11649
                     /locus_tag="LGK42_002714"
     CDS             10930..11649
                     /locus_tag="LGK42_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBK2658943.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            11646..12242
                     /locus_tag="LGK42_002715"
     CDS             11646..12242
                     /locus_tag="LGK42_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658944.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            12705..13205
                     /locus_tag="LGK42_002716"
     CDS             12705..13205
                     /locus_tag="LGK42_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK2658945.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            13934..14368
                     /locus_tag="LGK42_002717"
     CDS             13934..14368
                     /locus_tag="LGK42_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBK2658946.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            14460..14726
                     /locus_tag="LGK42_002718"
     CDS             14460..14726
                     /locus_tag="LGK42_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658947.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            14791..15732
                     /locus_tag="LGK42_002719"
     CDS             14791..15732
                     /locus_tag="LGK42_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBK2658948.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            15794..15964
                     /locus_tag="LGK42_002720"
     CDS             15794..15964
                     /locus_tag="LGK42_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658949.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(15995..16246)
                     /locus_tag="LGK42_002721"
     CDS             complement(15995..16246)
                     /locus_tag="LGK42_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2658950.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(16280..16477)
                     /locus_tag="LGK42_002722"
     CDS             complement(16280..16477)
                     /locus_tag="LGK42_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658951.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            16826..17674
                     /locus_tag="LGK42_002723"
     CDS             16826..17674
                     /locus_tag="LGK42_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBK2658952.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREAQDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            complement(17759..18094)
                     /locus_tag="LGK42_002724"
     CDS             complement(17759..18094)
                     /locus_tag="LGK42_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBK2658953.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            18328..>18465
                     /gene="nikA"
                     /locus_tag="LGK42_002725"
     CDS             18328..>18465
                     /gene="nikA"
                     /locus_tag="LGK42_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBK2658954.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YI"
BASE COUNT         3980 a         4990 c         5418 g         4077 t
ORIGIN      
        1 gtctgtgatt cgttcaccaa aactgcgact acagacaatc tgctgttttg ccggtggtgc
       61 ttcttccagg gatatgcagg actcgccgtt gagttcgcgt accgtacgct caagaatgac
      121 gctgaagttt ttccggatga atgccgtgtt agcctgcgcc agctgcagtg ctgtgttaat
      181 acccagcgca ttcagctttt ccgtcagtct gtgtcctact ccccagacct caccaactgg
      241 ctgcagcccc agtagcttca agatccgatt acggttttct gccgtcagcg cgaccactcc
      301 ggaaaactgt ggccattgct ttgttgccca ctgtgcactt ttagccagcg tttttgtagg
      361 cgcaatgccc acccccatgg tgagtcctgt ccagctcttt acctgttccc tgagctgatg
      421 accaaaaaac tccggagaga tgcaatgatt tatcccccgc aaatcaatga acatttcatc
      481 aattgagtag ggctcaactg cgggagaaag cgactccaga acagccataa cccgttggct
      541 catgctgtgg tacagcgcat aattgctgga aaatacatgt attttcttct ccaggcgcat
      601 ttgtctcacc tgaaaccagg gctgccccat tctgatgcca agggcttttg cctccgggct
      661 gcgcgcgatc acacagccat cgttattgct gagtacgatg accggttcgt tgcgaaggtc
      721 cgggcggaaa actttttcac atgaggcgta gaaactgttg atatcagcca gtgcaaacat
      781 cagcgtaact cccgcgtcct gtgtatcacg tgagtgacaa caccaaaaat acagatgttt
      841 tccggataca gtgtgcggaa ctccgggctg tctgaaaccg gctccagtgc cgggcgtggg
      901 cgcaacagca gtcgtttgac ggtgaactca ccgtcgatct cagcgataac gatgtccccg
      961 tgttgtggtt tttcggccct gtccactacc agcagatcac cattctgcac gccagcctgg
     1021 ttcatcgatt caccgctggc gcgcagaaag aaggtggctg caggtctgct gatgcaatag
     1081 ctgttcagat ccagttcctg ctcagcataa tcagtggcag gcgaaggaaa accggcctgg
     1141 caacgatcgg caaacaacgg tcgcacgtaa ctgtcatcgc ctgaagggtc agccggacgg
     1201 tgataaacgg tactcatgct gcaggcagcc agctgtcgtc ctgccagatt tcttccagca
     1261 gagacaggat acgttcttta tccttgtcgt tgcgggctcc tgtgacagat acagacgggg
     1321 cgctgccctg gtctatacgc agccagattt ccgggtagtg tggtttcaga cgttgttcga
     1381 tttcattctg tagcgcctga agtgtgcctg atttgaggtt ttttgtgctc tggcggtcga
     1441 aaagaatttc aatgcggatc atgttctcat ctcccttttc ttttgctgta tgtatataca
     1501 gtattctaat aactgaatcc gcacacagtc aaatgtcatt gttgtgacta aaaaacgctc
     1561 aattctctga gtgtgtggct gtatgcctgc tgtgggctgc tgtgtccagc ttatccacag
     1621 cattttgtgc acggttctgt ggacaaaata cctggttacc caggccgtgc cggcacgtta
     1681 accgggctgc atccgatgca agtgtgtcgc tgtcgccggc ctcctcaccc ggtcacgttt
     1741 cgtcgttcct cctccacgcg ctgcggcttc ggggccgcac ctgcattcgt atgcggtcgc
     1801 ccggtaacag gtgcggcacg gcctgatgga ggccgcatgt gagaggagaa ttcccatgcc
     1861 aaactggtgc tcgaatcgta tgtatttttc tggtgaaccg gcacagatcg ctgagattaa
     1921 acgactggcc agcggtgcag ttacaccgct ttatcgccgc gccacaaatg aaggtattca
     1981 gctgtttctg gccggaagtg ccggacttct gcagatcact gaaaatatcc gctctgaaca
     2041 gtgccccgga gtgacggctg ccggacgtgg tgctgtatcg acggaaaata tcgcgttcac
     2101 ccgctggctg acacacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     2161 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggatga
     2221 tgtcagggaa accatcactg tgcatttcac cgcaaaaaga ggtgactggt gtgacatctg
     2281 gggtagcgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtag tgccggaaaa
     2341 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     2401 ctttaatggt ggtgttctga acggtgttcc ttctgcatat cactggtata cagaacggta
     2461 tggcgtgaag tggccttgtg ggtatgacct gaatatcagt agtcagggag acaactgcat
     2521 tcaggtggat ttcgacacgc cgtggtgtca gccggaaagc gacgttgttg cagcattaag
     2581 ccgccgtttc ggctgcacgc tggagcactg gtacgccgaa cagggctgca atttctgtgg
     2641 ctggcagttg tatgagcgcg gagagctcgt tgatgtgctc tggggggaac tggaatggtc
     2701 ttccccgaca gatgacgatg agctgccgga agtcaccgga cctgcgtgga tagtcgacaa
     2761 ggtggcgcat tacgggggat gaagtatgac ggagacgggc gggcaaccgc ccgtttcttt
     2821 tccgacaaag gatgtcgccg tgctcctctg tttactgcgc cggctgatgc ggcgcggcat
     2881 aaacgctgcc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     2941 tcccggctgg cgtcggtgac agtgtacgcc agcctgtcgc ccttttctga tgaacgccct
     3001 tcaagccgct ttcgcggcat attctcgccg tcaaaaagac gacggctgcg atattccacg
     3061 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc
     3121 acaggggtgc cctcccggtg ccctctgaga aagaaggagt gttgttatgt cccgttttgt
     3181 cctcggtaac tgcattgatg ttatggcgcg tattcctgat aacggcattg atttcatact
     3241 gactgacccg ccatacctcg tcggttttcg tgaccgacag gggcgtacca tcgccggcga
     3301 taaaaccgat gaatggctgc aaccagcctg taatgaaatg tatcgcgtac tcaaaaaaga
     3361 tgcgctgatg gtcagcttct acggctggaa ccgcgtcgat cgctttatgg ccgcctggaa
     3421 aaatgcggga ttcagtgtgg ttgggcacct ggtcttcacc aaaaactaca catcgaaggc
     3481 cgcatatgtg ggttatcgcc acgaatgcgc ctacgttctg gcaaaaggcc gtccggcgct
     3541 gcctcagaag ccattacccg acgtgctggg ctggaaatat tcaggcaatc gtcatcaccc
     3601 gacggaaaag cctgttacca gcctgcaacc actgattgag agcttcacac atccgaacgc
     3661 gattgtgctg gacccgtttg caggcagcgg ctcaacctgc gtggctgccc ttcagtccgg
     3721 acgccggtat atcggtatcg agctgcttga gcagtatcac cgtgccggac agcaacgtct
     3781 ggctgccgta cagcgggcca tgcagcaggg ggccgcaaat gatgactggt ttatgccgga
     3841 ggctgcgtaa atgaactatg ccggacatga aaaacttcgc gccgaagtgg cggaggtggc
     3901 caatgccatg tgcgacctgc gtacaaccat gaatgagatg gagcggcggt acagctttaa
     3961 tgctgacacc ctgccggaac gtctggtgcg tcagacgttg tttcgcgcaa accgcctcct
     4021 gatggaggca tataccgaaa ttctggaact ggattcctgc ttcaaagatt gaggggagag
     4081 agtatgtacg gaacatgcga aatactctgc cgggagctgg cagcaaagta tccggcagac
     4141 acgccactga tgctggttgt ctggtcccct gaagagattc aggccctcgc tgacggaatg
     4201 gatatttccc tgtccgatca tgaaatcagg accgtcctgg cgcgcctgga ggacatcccg
     4261 gaagaccagc ggattgagtc cggtatttct tccggcgtgg cgatggagat tatcagcaac
     4321 gtgagagaaa accgccaggt gaccgtccct gctgaactgc tggcgtccct gattcagacc
     4381 gctgaacagg cactgtggaa acgtgaatgg gccgcccgtg ataacggcct tgccgtcccg
     4441 gaatgtgtta cccgccgtca ggcagtgatt aatcaggccc gcaccctgct gaaaaacaac
     4501 acacacgaaa acaactgatg ttatcgccgc ctgccctgcg ggcggcgata cagggagaac
     4561 gcttaatcat gaacaaaacg ctgaatgcac tggtttgtcg tcacgcccgt aacctgctgc
     4621 tggcgcaggg ctggccggaa gagacggatg ttgaccagcg aaatccgaac tatccgggct
     4681 ggatcagcat ttacgtgcgg ctggatgcgc cccggctggc gacgttactt atcaaccgtc
     4741 acggcggcgt actgccgccg ctcctggcct ccgccattca gagactgacc ggaaccgggg
     4801 cggaactggt actgtccggc agtcagtggc agtcgctgcc ggtacttccg gcagacggaa
     4861 cgcaggtgtc tttcccgtat gccggagaat ggctgaccga agacgaaatc agggctgttc
     4921 ttgatgcggt gcacgatgcg gtacgaagca tctgttacca ggtggcagaa gatgcgcggc
     4981 gtatccgtgc ggcgctgacc accaccggtc agacgttgct gacccgccag acgcgccgct
     5041 ttcgcctggt cgtgaaggaa agcgatcacc cctgctggct cgatgaagat gacgaaaacc
     5101 tgcccgtggt gctcgatgcc atcgtgaacc ggggagcacg tttttcgtcg gtggaaatgt
     5161 acctggtcag cgattgtatt gagcatatcc tgtcctgtgg gctggcctgc gatgtgctgc
     5221 gtatacccga tgaaccgccc cgccgctggt ttgaccgtgg tgttctgcgg gaggtggtcc
     5281 gggaagcccg gaccgaaatc cgcagcatgg cggatgccct ggcaaaaatc cggaaatgat
     5341 gacactggcg gggtaaatcc ccgccttttt cctgccggtc cccggacaga gcacaaagga
     5401 agtcgccgcc ggctccgctt tacccggcca tgcggggcat ggccttgtgg gttttcagtt
     5461 atgtggccac cgtgtcgtgt gcgggctgtg ccgtgcctcc atcttagccg ggctggcagg
     5521 gatgcaaggg tacgcttcgc cgctgcggtc acccggtccc tccttcccgt ctgccgtgat
     5581 tttccggtcg ttcacccgct caggtttcgc ggtctcaccc tccaactccc gccagccgtg
     5641 tgcgtcaggc tgcggcttcc cttgcgccct gtgcatcccc gccttatcgc ccggctttta
     5701 tggaggcacg gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa tacatcatgc
     5761 aatatgcgaa acctgtcact ctgaatgttg aagagtgcga ccgtctgttt tttctgcctt
     5821 acctgtttgg ccaggatttt ctgtatgtcg aagcgtctgt gtacgcgctg gcgaaaaaaa
     5881 tgatgccgga atatgaaggc ggattctggc acttcatccg cctgccggac ggtggcggct
     5941 acatgatgcc ggatggcgac cgtttccaca tagtgaacgg tgcaaactgg tttgaccgta
     6001 ccgtgagtgc tgatgcctgc ggcatcattc tgacttccct tgtgattaac cgccagttgt
     6061 ggctgtacca cgacagcggt gatgcaggac tgacccagct ttaccggatg cgcgatgcgc
     6121 agttgtggcg tcacatcgaa tttcaccctg aatgcaacgc gatttacgcg gctctggact
     6181 gattaacgga cggggcggca atgccgcccc tgtaaaggag acgagaaaat gtactgtact
     6241 gttaaagaaa ttatccgcga agtgctggat acagacgtgc cggacagtga atgcgttttt
     6301 gccgtggtgc tgacccgtgg ggatgtgcgt cacatagccc aggactggaa cctgtcagac
     6361 gatgagctgg aaaccgtcat gcagcggctg gacgatgcct ttgagcatgg tgcggatgtc
     6421 agcgttgttc acgacgttgt tcgtgaactg atggaagaaa agcgcgtcag ccgtcaggtg
     6481 acagtcccgg cggtgatgct ggaaaaagtg gtggcgctgg caggcagtga aatgaagcgc
     6541 ctgtatgccg tcgggagtga gaacgggggc gacggtgacg cattcgtcag ggaagaacgc
     6601 gaagcgatgg acgttgtgtt acaggcgctg gacggggagc acatgtcatg aatatcagca
     6661 cagaaacccg cgaaattctg cgcaattaca aagccgtgat taatgcgcgg cgtcgtgaga
     6721 tggggcagaa accgctcacc actgcgcaga ttgttgatga aatctgcgat tttgtggtga
     6781 atcagcaggc ggttttcctc ggtggtcact atatccttca gggcagcaga aacaggtgat
     6841 acagggcagg cggtggaggc cgcctgtcag cccgcgcgaa aacccctggc aggctgacgc
     6901 ctgccggaaa tgcggggtgc agcgcctgcg gcgctgccgg cccggctttc cggtcagaag
     6961 atagtgtatg gctgtatgcc aggaacaggt gcgatattcc gtgacactgt cactgtttct
     7021 ggctgtcttt tattgcgccc acttcggctc ccggctgcct ggcggcatcc ggtcgtcagg
     7081 cccgactacg acaggcagct gcttgcgcac ccgccagtct ccgccgaacc cgctggcgcg
     7141 gtttcgggct gtcgctacgg tccgatgcgt gaaaaaccgg gcttgcagcg accgttccgg
     7201 tcgcgccgcc tctgtcgcgc cgtgacctgc gctccctgca cacctgacgg tgtgtgagga
     7261 aactgcgcct gatggcgcag cagtcttccc tcctgccgcc acctctgcgg cctgtttccc
     7321 gctgtggact ttccgggggg cagacgtaac cccctcccgt tccatctgtc cagggtgaga
     7381 tgcaccggcc acaaaatttt tagccatgaa cccggcaaaa aattttgcgt ccgcccccgg
     7441 acagctgtcc cgtgaggcgg taaacgggcc cccatcggaa aggtccactg cggggagaaa
     7501 cccctgtgaa gtggacggca ggaggagctt tcgctcctct gaaggggcac atgaacgtcc
     7561 cgtctccggt atccggttta tctcacatct ttcagtcagg aggttcttat gagtgcacgt
     7621 ggtatcaaca aggtcatcct cgtcgggcgt ctgggcaatg atccggaggt ccgttacatc
     7681 cccaacggtg gcgcagtggc aaacctgcag gtggccacgt cagaaagctg gcgcgacaaa
     7741 cagacggggg agatgcggga gcagacggaa tggcaccgtg ttgtgctgtt cggcaagctc
     7801 gcggaagtgg caggtgaata tctgcgcaag ggcgcgcagg tctacatcga ggggcaactc
     7861 cgtacccgta gctgggacga caacggcatc acccgctaca tcactgaaat tcttgttaag
     7921 accacgggca ccatgcagat gctggggagt gcaccacagc agaacgctca ggcgcaaccg
     7981 aagcctcagc agaatgggca gccacagagt gctgacgcga cgaaaaaagg tggcgcgaaa
     8041 acgaaaggcc gtggacgtaa ggccgcgcag cccgagcctc agccgcaaac gccggagggg
     8101 gaggattacg ggttttcaga cgacatcccg ttctgatcgg gctgactgtg acaaccgccc
     8161 cgccctgtgc ggggcatcac cggagatatg aggatgagcg aatatttcag aatacttcag
     8221 ggactgccgg acggctcctt tacccgcgaa caggcggaag ccgttgccgc acagtaccgg
     8281 aacgtcttta tcgaggatga ccacggagaa cattttcgcc tggttgtccg taataacgga
     8341 acaatggtct ggcgtacctg gaattttgag gacggtgccg ggtactggat gaaccatgcc
     8401 atccgtgatt tcgggattct taagtaagag aggtgccgga cgcgcgaacg tccggcagga
     8461 cataagcaat tataagggga tgattatgcc tgtaacgaag tgtgaaccag aaaccacccg
     8521 caaagcaagc cgtaaatctg caaaaacgca ggaaactgcc ctgtctgccc tgctggcgca
     8581 gacggaggaa gtgagcgtgc cgctggcctc actgattaaa tcaccgctga atgtgcgcac
     8641 ggtgccgtat tctgtggagt ccgtcagcga actggctggg tccattaagg gcgttggcct
     8701 gttgcagaat ctggtcgtgc ataccctgcc tggtgaccgt tacggtgtcg ccgcaggtgg
     8761 tcgccgactg gctgcactca acatgctggc agagcgcaac atccttccgg ctgactggcc
     8821 tgtccgtgtg aaggtcatcc cgcaggaact ggcgactgcc gcatcgatga ccgagaacgg
     8881 tcatcgtcgg gatatgcacc ctgccgaaca gattgccgga ttccgcgcat tggcgcagga
     8941 aggcaaaaca cctgcacaga ctggcgacct gctgggctat tcaccccgcc acgttcagcg
     9001 aatgctgaaa ctggcagacc ttgcgcctgt catcctcgat gcgctggcag aggaccgcat
     9061 caccaccgag cactgtcagg cgctggcgct ggagagcgac accgcgcgtc aggtgcaggt
     9121 gtttgaagcg gcctgccagt caggatgggg cggtaaaccg gatgtgcggg ttatccgcaa
     9181 cctgattacg gaaagtgaag tggcagtgaa ggacaacact aaattccgct ttgtgggggc
     9241 tgatgccttc tcgccagacg aattgcgcac cgatttgttc agcgatgacg gggacggtta
     9301 tgtcgaccgc gtggcgctcg atgccgccct gctggaaaaa ctccaggctg ttgcagagtt
     9361 ccttcgggaa gccgaaggct gggaatggtg cgccgggcgc atggagcctg tcggtgagtg
     9421 ccgtgaggat gccggaacat accgctgtct gccggagccg gaagcggtgc tgacggaggc
     9481 ggaagacgaa cgcctgaacg aactgatgac gcgttacgac gcgctggaaa accagtgtga
     9541 ggaatccgac ctgctggaag cagaaatgaa gctgatgcgc tgcatggcga aggtcagagc
     9601 gtggacgccg gagatacgtg ccggaagcgg tgtggtggtg tcctggcgtt atggcaacgt
     9661 atgtgtccag cgtggtgtgc agttgcgcag tgaagatgac gcgactgacg acgctgaccg
     9721 cacggaacag gtgcaggaga aagcgtcagt ggaggaaatc agcctgccgt tgctgacgaa
     9781 aatgtcctca gagcgcacgc tggcagtcca ggcggcactc atgcagcagc cggacaaatc
     9841 tctggcactg ctggcatgga cgctctgcct gaatgtgttt ggcagcgggg cgtacagtaa
     9901 accagcacaa atcagcctgg aatgtgaaca ttattcgctg accagcgatg cgccatcggg
     9961 gaaggaaggt gccgcattca tggcgctgat ggcagaaaaa tcccgtcttg cagccctgct
    10021 gccggaggga tggtcacggg acatgacgac attcctgtcc ctcagtcagg aggtgctgtt
    10081 atccctgctc agtttctgca ccgcatgcag ccttaacggt gtccagaccc gtgagtgtgg
    10141 tcacacgtca cgcagtccgc ttgactcgct ggaaagcgct atcggattcc acatgcgcga
    10201 ctggtggcag ccgacaaaag caaacttctt cggacacctg aaaaagccgc agattatcgc
    10261 agccctgaat gaggccggac tgtccggtgc cgcacgggac gcggagaaga tgaagaaagg
    10321 tgatgcggct gaacatgcag agcaccatat gaaagacaac cgctgggttc caggctggat
    10381 gtgtgcacca catccacaga cagatgccac tgaacgcacc gataacctgg ctgatgccgc
    10441 ctgatgaaca accacaccgc cccgccggag acggggcggc agcaagggag ataccgtgat
    10501 gaaaactgaa ctgaccctga atgccctgca gtccatgaac gcacaggaat atgaagatat
    10561 ccgtgctgcg ggaagcgata tgcgccgtaa tctcactcac gaggtgatgc gtgaagtgga
    10621 cgcaccggct aactggatga tgaatggcga gtatggcagt gagttcgggg gctttttccc
    10681 cgtccaggtt cgtttcacgc cagcccacga acgtttccac ctggcattat gttcgccggg
    10741 agacgtctct cagctctgga tgctggttct ggtgaattgt ggtggacagc ctttcgccgt
    10801 cgttcaggtg caacatatct tcacgcctgt cgctatcagt cacacgctgg cgcttgccgc
    10861 gacacttgat gcgcaggggt acagtgttaa cgacatcatc catatcctga tggcagaagg
    10921 aggtcaggca tgagcgcacg ttcacgggca ctgatccccc tcagcgcaga gcaacaggcc
    10981 gccatgcagg ctgtggctgt caccgaacaa cgtcgtcgtc agggacgcac actttcagca
    11041 tggccttatg ccagcgcttt ctttcgctgc ctgaatggca gtcgccggat ttcgctgacc
    11101 gatctccgct tttttgctcc tgcgctgacg aaggaggaat ttcatggcaa ccgtctcctg
    11161 tggctggctg ccgtggataa actgattgaa agttttggtg aagtctgtgt tcttcccctg
    11221 ccatccgatg cggggcatcg tctgttcccg tccgttcctt ttcgtgaagg tgagcggcgt
    11281 cgtcagaaaa ccacgctgac agagcagaaa tacagccgcc agcgggaacg tgaggcagaa
    11341 cgacgggaac tggaatacca gacatgtttt gctcaggcgc agattgacct tgcgtttcat
    11401 actcccgcca cggtcggaag ctggttgtcc cgctggtctg gtgttgttga ggagcatgat
    11461 ctggaaacga ttttctgggg gtggtgcggg cgttttccat cactgtcatc atttgaccgg
    11521 tttttctggc aggaggaacc actctggcgg ctgatttttg aagccggtga ggccggtcgt
    11581 ggtgcaccgg tacaggtacg tgcacttgag cagtggatga tcccgaacaa gctggagaac
    11641 gcaatatgat gaaatcagac gaacaatatc aggttcccgt gtggatgcga cctctgttgc
    11701 cgttgctctg caataccggt ggtaatgatc cggaggaact gctgaatgat acagaaacca
    11761 ccgccagtgc gaatattgtc cgttatgtac tgatagttgc agtgcggtcg cagattgatc
    11821 tgctgcagct actgtacagg aaaggactgt tgcgcacaga gataccaggt ggcttttcac
    11881 cggaagaagc gcaggaactc ctggataatc tggtgcgcag ccatatcagc aaggcgttgt
    11941 cgggtgagcg aatggcagcc cgtgacagaa atgccgatct gacctggatt cgccagcaac
    12001 tggtcgatgc cgcctggttt gtccgtgcca cactggaagc gcatgggatg ggcgtcggaa
    12061 atgagagtcc ctctgctccg ccggagacaa tgccggacat acagacacgg gaactggtta
    12121 tgttgatcaa gcgactggca tcatcgctga aagcggtgaa acccgacagt agcgtggtgc
    12181 gtgaagcgca ggactggctg tgcgacagaa aacttgtgga tatcacagat attctccggt
    12241 gaaccagaca tgtatatttt acgcagggac acgccgatca tgatcgtttc atatcagggg
    12301 tggtctggag gtcatgcggc gtgtcctctg cactcgccgg aataaggaag tcgccggcgg
    12361 ctccgctttt acccggccat gcggggcatg gccttgtggg ttttcagctc tgtggcctca
    12421 gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg gctggcaggg atgcaagggt
    12481 acgcttcgcc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
    12541 tcacccgctc agatttcgcg gtctcaccct ccaactcccg ccagccgtgt gcgtcaggct
    12601 gcggcttccc ttgcgccctg tgcatccccg ccttatcgcc cggcttttat ggaggcacgg
    12661 caccgcccgg tgtcgcagaa catgtgacta tggaggattc gggaatgtct gttgttgcac
    12721 ctgctgtata cgttggaacc tggcacaaat acaactgtgg aagcatcgct ggccgctggt
    12781 ttgacctggc cacgtttgat gatgaacgcg actttttcgc cgcctgccgc tctcttcacc
    12841 aggatgaagc cgatcctgaa ctgatgtttc aggattatga gggattcccg gggaatatgg
    12901 cctcagaatg ccatatcaac tgggcctatg ttgaaggctt ccgccaggcg cgggatgaag
    12961 gctgcgaaga ggcttatcgt ctctgggtgg atgataccgg tgagacggat tttgacacct
    13021 tccgcgatgc ctggtggggc gaggctgaca gtgaggaggc ttttgcggtt gagttcgcca
    13081 gtgataccgg cctgctggct gacgtgccgg agacggtggc gctctatttt gactatgagg
    13141 cgtatgcgcg ggatttattc ctggactcct tcacctttat tgacggtcat gtgttccgtc
    13201 ggtgatttac tcccccgctc cggcgggggt atggcctgcc aggccgccgg aaaaccctgg
    13261 caggcttacg cctgcctgaa atgcaggagg gccgcgcctg cggcgctgcc cttcctgccc
    13321 gtcatggtgt gaggcatttt tctgttatgt cgtttctgtt gttctgattt ccggaggcgg
    13381 ggacaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga ctccgacagg
    13441 cagctgctgg cgcacccgcc agtctccgcc gaacccgctg gcgcggtttc gggctgccgc
    13501 tacggtccga tgtctgaaaa accaggcttg cagcgaccgt tccggtcgcg ccgcccctgt
    13561 cgcgccggaa acggcgctcc ctgcacacct tacggtgtgt aaggcaactg cgcctgatgg
    13621 cgcagtcaac ccttccgccg ccacccctgc ggcctgtttc ccgctgtgga ctttccgggg
    13681 gctggagtaa ccacatcccg tttcagcagt ccaggggaag atataccgtc cacaaaattt
    13741 ttagccataa acccggcaaa aaattttgcg tccgcccccg gacagctgcc ccgtgacgtg
    13801 gtgacggacg ccccatcgga aaggtccact gcggggagaa accccgaaga agtggacggc
    13861 agaaggggct ttcgcccctc cggggagcaa ccggtcggcc cggcgacctg attcagcaga
    13921 aggagatgtt ttcatgacag tcagcagcac gatttccgtt ttttgccgcg acggggtgtt
    13981 ccgtaccgtt tactgccacc tgcacgggga gccgacctgg aacggtcgca tcctgcatac
    14041 tcactatgcc accggtcagc aggccgaagc cctggttgaa cacggtgata tccgttgcct
    14101 cggtccccgt tgtgacaaac ccgccgggca tacgcttcag aacccggtgg acggtgtgac
    14161 ggcttattac ggacgtgaca gtggtttccg gatggacagt gaggcgcgtg agtaccgttc
    14221 tttcagggag gctattgcca ctgaaagcac tgaagaggtg cgcttccatt atgtgttcat
    14281 cgacggctac tggaaggtga tgtaccgcac gccggaaggc tggaagatga aagcgctcgc
    14341 gctggcactg cgtcgctgtc cgaaatgaaa aaaaggcagg gcgataaacc ctgcctctct
    14401 ccgccgttac ttccccgcca ggaagagcgg cagtcatcaa caacataagg agtaatattg
    14461 tgagaccatc aattatcttc gcaaccgccg agtatgtaaa gcgtctgcgt gaagagtgcc
    14521 tgcgggagaa taaacccctg caccgccata cccgcttcag acgtcaggag ctggcacagg
    14581 atgagattaa cccggacgtc ctggcgatga gcggccatat cgccagacgc tgcagtgagc
    14641 agaagcgggt gcgtatcccc gctatgaaag tcagcgaatg gggccacctg ctccgcgcgc
    14701 ttgaaattga gcgggtctgc cactgattaa cccttgccgt ctgcctgtgc aggcggcagg
    14761 atacgttcag acatctcacc aggaggaacc gtgagtaaga agaaaaacac cacaacgccc
    14821 acgccgcatg atgccgcgtt ccggtcgttc ctggcgaatc ccgacgtcgc cagagatttt
    14881 ctggaactgc atcttcctgc ggagtaccgg cagttgtgcg acctgtccac gctgaagctg
    14941 gaacccgcca cctttgttga gccggacctg catcagtacg ccagcgatat cctctggagc
    15001 gtgaaaacca ccgggggtga agatggttat gtttatacgc tcatcgagca ccagagcacc
    15061 gaaaatctgt acatgccttt tcgcatgtta cgctacagtg tggcggcgat gcagagacat
    15121 ctggagcagc acaaaacgtt gccactggtc attccggtgc tgttctatca cggtgagcgc
    15181 agcccgtacc cgtacagcat gaactggctg gactgttttg agaatccggc gcttgcggct
    15241 aaaatataca caaagccgtt tccgctggtt gatatcactg tcgttgatga caatgaaatc
    15301 atgaaccatc gccggatggc cgcactgacg ctgctgatga agcatatccg tcagcgggat
    15361 atgctgatgt gtctggacaa cctggtcaga gcactgcagg acatccagga tgaagagcag
    15421 attacagttc ttttcaacta tctgcttaat ggcagcgaac atgtaacggt tgaatttttg
    15481 cagacgctgg cgcagcgact gccgcagcac gaggacagca ttatgacact ggcagaacgt
    15541 cttaagcagg aaggtatcca gcaaggtatc cagcagggta tccagcaggg tatccagcag
    15601 ggagttcagc agggggcgct tcaaaaagca cgggaaatcg cccgggagct ccggaacgcg
    15661 ggaatgccgg cagcacaaat ctgtcagctg accggacttt ctgaagctga actgaagaac
    15721 atcactcact gatgagagcc aaaaggcccg gcaaaatgcc gggccttttg ccagcaggag
    15781 cagagaaaac agtatgacga ttgcagaacg tcttatacag aaaggttttg aaaaaggtgc
    15841 tcttgaagtg gcgcgggaaa tagcctgccg gcttcgggat atgggctgga cgccggaacg
    15901 gattcaggag gccacgggac tttccggtga agaactgaaa aaactgtttc ctgatgagca
    15961 gtagcctggc attctgccag gctgtgaacc acgctcacac atcctctgcc gtttccccca
    16021 gttcattttc cagacgcctg atgatatctg ccttgcgcat cccctgcatg tgaggcagca
    16081 ggtcacggac tgaaagtgcc tgggtggtcc tccagacggt gacatccacc tgttccgcat
    16141 tttcccaggc gtgccatgtt ctctgtgtca cgccaaattc tctggccgct ttttcgcgtg
    16201 accagagcat gctttttcgc cagattcgca gttcccagcc ggtcataaaa cacctttgaa
    16261 aaagtgaaaa aacttcactt tattttatca ccataaccac caaatcagcg cgaaaaataa
    16321 gtgtgaaaaa gtgaaggaat ttcacttttt ttgattgtag gtgtcgtgct gttttctctg
    16381 ttttcacctg tgcgggagtg gtgtgccagt gaccaccaca tgccattgtt gcgccgtcgt
    16441 ttttcttcag tcgcgccgga tgcggcgctc cctgcacacc ttacggtgtg taaggccact
    16501 gcgcctgatg gcgcagctgt ctctgcctgt ctgccgccgc actgcggcct gttctccgca
    16561 gatggacttt ccgggggtac atacacgacc acatcccgtt tcagtcgtcc aggggagaga
    16621 tgtaccagcc acaaaacttt ttgccgcagg cgtcaaaaac ttttgcgtct gcccccggac
    16681 aactgccccg tgacgtggtg agcgtccccc gtcggaaagg tccactgcgg ggaaatcccc
    16741 gaagacccgg acggcagaag gagcttcgct cctccgggaa atgacctggc tgacccggca
    16801 gtcgtgacga tgaggagaca atgtgatgaa tcagacttta cccactgctg acctgaatac
    16861 tgccggcacg acagatgtta ttccgtctgt ggccatcgac cgcatcatcg cgcagcgtaa
    16921 cgaaggtatt gcactgttca tgcaggcgat ggaatgcctg gcgacagcgc gcaggattct
    16981 gcttgatgcg tcaggtgata tttttcttta cggttttgaa gactgcgtga ccgactccgt
    17041 tcggtgcatg gataaaccgg aagaagcgaa aaggaatatc acccgtcttg ccgaccggaa
    17101 aatctgggac cgcctgatga cggatacggg gatgtacacc ttcatgagtt catgtcagcg
    17161 tgatgagtgg aacagccagc tgatgagcga cacctgtcct gaaatcaccc tggacaatgt
    17221 actggcaact tttcgccatc tgaatgccag taagatgcag acatttgaac agggactgat
    17281 tgatgtctac cggaaattgt catgggatta cagaaccaat aatccctgcc gtctgggcaa
    17341 gagaatcatt attgaaaacc tgctgtaccg ctggagtaac gggcgtgtta cgctggactg
    17401 cagcggacgg gaggcacagg atgacctggt acgtccgttt tatctgctgg aggggcgcaa
    17461 cgttcctgac ttccggagca gcatcggggc gcagtacggt gaatttatcg ggaacggcga
    17521 caatgtcggt aagctgttag aaggggaata ttttacggtg cgtggctacc agaaagggac
    17581 cgtacacatt gtctttaagc gtcctgacct tgttgaaaaa ctgaatgata ttattgcacg
    17641 gcattatcct ggttcattgc cgccacgagt ctgattaaac agaaaagccc tggtatttat
    17701 gccggggctt tttttctgta atcatcataa tatcagaaca tgatgtgaca ttgtcacatc
    17761 aattgttgtt catatcgatg ccatctttcc tggctgaatc tgtcttttta gatgctgttt
    17821 tagaggaaga acggcgggat ggctttttcg attctgcttc ccgaataatg tcacgaagtg
    17881 ccttctctcc tacttcatac cctgcatttt tgagcgtgtc acgaacatct gccagtgtat
    17941 aaccctttgt ccttaccaga acaataatgt catcacgaat ggcatcaaga aaatcacgca
    18001 atgttttccg ttgagcggtg agatctggca attccgataa agcagctttt gccagctgga
    18061 tgtcatcatc tgagtaaaat tttttagaag ccattggcac tttctctccg tttcatgttg
    18121 aagtctgatt ttatcatcaa aaactgactc ctggcagggc tgcgccctgc ctctgccgtc
    18181 tgcataagac tatgatgcac aaaaataaca ggctataatg gcctgaaaaa cgggacagga
    18241 tgtgcaattg taataccgtc acacgcgacg ctattacaat tgccatctgg tcagggcttc
    18301 gccccgacac cccgtaagga gcctgaagtg agtgatagtg cggtaagaaa gaaaagtgag
    18361 gtacggcaaa agacagttgt caggacactc agattctctc ctgttgagga tgaaaccatc
    18421 aggaaaaaag ctgaagattc cggacttact gtatctgctt acatc
//
LOCUS       DAFAVF010000079        18212 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFAVF010000079 DAFAVF010000000
VERSION     DAFAVF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.079"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..205
                     /locus_tag="LGK42_002726"
     CDS             <1..205
                     /locus_tag="LGK42_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2658955.1"
                     /translation="TLSYYESGRSTPPTDVMMNILQTPQFTKYTLWFMTNQIAPESGQ
                     IAPALAHFGQNETTSPHSGQKTG"
     gene            321..1334
                     /locus_tag="LGK42_002727"
     CDS             321..1334
                     /locus_tag="LGK42_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2658956.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     DTGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(1599..1916)
                     /locus_tag="LGK42_002728"
     CDS             complement(1599..1916)
                     /locus_tag="LGK42_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2658957.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            2331..3230
                     /gene="yegS"
                     /locus_tag="LGK42_002729"
     CDS             2331..3230
                     /gene="yegS"
                     /locus_tag="LGK42_002729"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBK2658958.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(3312..4085)
                     /locus_tag="LGK42_002730"
     CDS             complement(3312..4085)
                     /locus_tag="LGK42_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK2658959.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4191..5231)
                     /gene="gatD"
                     /locus_tag="LGK42_002731"
     CDS             complement(4191..5231)
                     /gene="gatD"
                     /locus_tag="LGK42_002731"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK2658960.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5280..6635)
                     /locus_tag="LGK42_002732"
     CDS             complement(5280..6635)
                     /locus_tag="LGK42_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK2658961.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(6639..6923)
                     /gene="gatB"
                     /locus_tag="LGK42_002733"
     CDS             complement(6639..6923)
                     /gene="gatB"
                     /locus_tag="LGK42_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBK2658962.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(6954..7406)
                     /gene="gatA"
                     /locus_tag="LGK42_002734"
     CDS             complement(6954..7406)
                     /gene="gatA"
                     /locus_tag="LGK42_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBK2658963.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(7416..8678)
                     /gene="gatZ"
                     /locus_tag="LGK42_002735"
     CDS             complement(7416..8678)
                     /gene="gatZ"
                     /locus_tag="LGK42_002735"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBK2658964.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(8707..9561)
                     /gene="gatY"
                     /locus_tag="LGK42_002736"
     CDS             complement(8707..9561)
                     /gene="gatY"
                     /locus_tag="LGK42_002736"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBK2658965.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(9871..10923)
                     /gene="fbaB"
                     /locus_tag="LGK42_002737"
     CDS             complement(9871..10923)
                     /gene="fbaB"
                     /locus_tag="LGK42_002737"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBK2658966.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11180..12457
                     /locus_tag="LGK42_002738"
     CDS             11180..12457
                     /locus_tag="LGK42_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBK2658967.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            12454..13458
                     /locus_tag="LGK42_002739"
     CDS             12454..13458
                     /locus_tag="LGK42_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK2658968.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            13455..14420
                     /locus_tag="LGK42_002740"
     CDS             13455..14420
                     /locus_tag="LGK42_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK2658969.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(14394..15140)
                     /locus_tag="LGK42_002741"
     CDS             complement(14394..15140)
                     /locus_tag="LGK42_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2658970.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(15192..16010)
                     /locus_tag="LGK42_002742"
     CDS             complement(15192..16010)
                     /locus_tag="LGK42_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBK2658971.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYSYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(16075..16875)
                     /gene="thiD"
                     /locus_tag="LGK42_002743"
     CDS             complement(16075..16875)
                     /gene="thiD"
                     /locus_tag="LGK42_002743"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK2658972.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(16872..17660)
                     /gene="thiM"
                     /locus_tag="LGK42_002744"
     CDS             complement(16872..17660)
                     /gene="thiM"
                     /locus_tag="LGK42_002744"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBK2658973.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVATCCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(17695..17791)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(17883..18155)
                     /gene="rcnR"
                     /locus_tag="LGK42_002745"
     CDS             complement(17883..18155)
                     /gene="rcnR"
                     /locus_tag="LGK42_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBK2658974.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLIREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         4586 a         4684 c         4335 g         4607 t
ORIGIN      
        1 cacgctgagt tactatgaaa gtggtcgttc aacacctcca acagatgtca tgatgaacat
       61 cctgcagacc ccacaattca ccaaatacac tttatggttc atgaccaacc agatcgctcc
      121 tgagtccggg caaattgcgc ccgctctcgc acactttggg caaaacgaaa caacgtcgcc
      181 ccactccggt caaaagactg gttaacaatt catcgtgaat atattcatta caagtgccta
      241 ctattggtgg ctaaatttca gccaccacga aaaaagcgat tagtagtcgc aaaaaaacac
      301 accactcgga gggttttctg atggcaatca aaaaactcga tgatggtcga tatgaagtgg
      361 acatccgccc tactggacgt aatggaaaac gcatccgtag gaagtttgat aagaaaagcg
      421 aagctgtcgc tttcgagaaa tacacgttgt acaaccacca caataaagaa tggctatcaa
      481 aaccaacaga caagcgacgt ctgtcggagc tgacacagat ctggtgggat ttaaagggta
      541 aacacgaaga gcatgggaaa tctaatcttg gaaaaattga aatcttcaca aaaataacga
      601 atgacccatg cgcatttcaa attacgaaat cgcttatcag ccagtactgc gccacccgaa
      661 gaagccaggg tattaaacct tcgagtatca atcgtgattt gacatgtatt agcggcatgt
      721 ttacagccct gattgaagcg gagttattct ttggtgagca ccctatcaga gggacaaaaa
      781 ggcttaagga ggaaaaacca gacacaggct atctcacgca ggaagaaatt gccttactgc
      841 ttgcggctct tgacggcgac aacaaaaaga ttgcgattct ttgcctgagt actggagcac
      901 gttggggaga agcagctcgt ttgaaagcag aaaatatcat ccataaccgc gtcacgtttg
      961 ttaaaacgaa aacaaacaaa ccacgcaccg tcccgatctc agaggctgtt gccaaaatga
     1021 tcgcggataa caaacgaggt tttttattcc ctgatgctga ttaccctcgc ttcagacgaa
     1081 caatgaaagc aataaaaccg gatttgccaa tggggcaagc cacacatgca ctaaggcaca
     1141 gctttgccac tcatttcatg attaatggag gaagtattat cacgctacaa cggatactag
     1201 gtcacacacg gattgagcaa actatggttt acgctcattt tgcgccagag taccttcagg
     1261 acgccatttc tcttaatccg ttaagaggtg gtactgaggc cgagagtgtc cacacagtgt
     1321 ccacagtaga gtaacgttta agggctttca gtggtaattt atgccgctca aacccgcatt
     1381 gtaccgttga aagcccctac tggtgacacc ctaaatctcc cttacacggg cttatttttt
     1441 atgcataagc cctatccctg gtcaccgtct tccattgacc acatcgatag aatctccctt
     1501 catagcacga tgcctttcac gtaacggcat cgtgctcgca caggttccgg ctaagcacaa
     1561 ccagaacgcg catgtttgac gcttaccaaa aaatattctc actctccaca tttgaatgtc
     1621 agacgagcga cgccatgtaa tcctgcacct tctgtcttca ggtcaactat ctgcattttt
     1681 ttgccctgag taacacagaa atgggctgca tcatttttta ctatattttc tgcaccagat
     1741 attctgcccc tggctaaaga agcttcggct tcggtgtagt attggttatc gagtttacgc
     1801 tgaatattac ttttatatgc aagaccaaat ttaccgatac ttgtctcatc attatgcaca
     1861 gcacaaccag acataataaa aatactaatt aatgatatag cagctatctt tttcatctca
     1921 ccttccccca ttaaatacca acgacactct ctagtgttta aatataataa tggcatgatt
     1981 attataattg aataggatta taataaatgt tctgtacaac atttcctaca taagtaggaa
     2041 ttacggacat tgaggccctt cagggtaact ccatgggggc tttaatatat ttggaatagt
     2101 atatttaaga tgccactgtt tggttgaata ttaggcatat gctctttttt gaaatttatc
     2161 ggtggcagcc gttagtattc gctgtcccca ttgcaagctc ctggtggtaa ccactgaatc
     2221 ctccatactt gaactgactt tttatcctcc gactttcatc ctgttctgac tccacctttt
     2281 gttttctgct ctacactatc tacagaccaa tcataaaggc acatacgatc atggcagaat
     2341 ttcccgccag cttactgatt cttaatggca aaagtactga caatctaccc ttgcgcgaag
     2401 caattatgct gttgcgtgag gaaggaatga cgatccatgt gcgggtcacc tgggagaaag
     2461 gcgatgccgc acgatatgta gaggaggccc ggaagttggg cgtcgcaacg gtgattgccg
     2521 gtggtggcga tggcaccatt aatgaagttt ctacggcgtt gattcagtgt gagggggatg
     2581 acatacccgc gctgggaatt ttgccattag gaaccgccaa tgattttgcc accagtgtag
     2641 ggattcctga ggcactggat aaggcgctga aactggcaat tgccggtaac gccattgcga
     2701 tagatatggc gcaggtcaac aaacaaacct gttttattaa tatggcgaca ggcggatttg
     2761 ggacgcgtat taccacagaa acgccggaaa aattaaaagc cgcgctgggt ggcgtctctt
     2821 acatcattca tggcttaatg cgcatggata ctctgcaacc ggaccgttgt gaaatccgcg
     2881 gtgaaaactt tcactggcaa ggtgacgccc tggtcattgg tattggtaac gggcgtcagg
     2941 ccggtggcgg tcagcaattg tgtccgaacg cgttaattaa cgatggcttg ctgcaactgc
     3001 gcatttttac cggcgatgaa attcttccgg ctctcgtatc aaccttaaaa tctgacgaag
     3061 ataacccgaa tattatcgaa ggcgcttcgt cgtggtttga tatacaagcc ccacacgaaa
     3121 tcacttttaa tcttgatggc gaaccgttga gcggacaaaa ctttcatatt gaaatacttc
     3181 cggcggcgtt gcgttgtcga ttaccaccag attgtccatt attgcgttaa tcaaactatt
     3241 ctctcttgaa ccagggagaa tccgtaatgc cggatgcggt gtaaatacct tatccggcat
     3301 acagaacaat actacaccgt aattaatttc acccgtgtct tctgcaatgc cagtttatcc
     3361 acctctgata cagcatcatc ggtaataata atgtcaattt tttcgattgg cagtacctga
     3421 ttaaaaccgc gacggttaaa tttcgatgaa tcgagaacgg caatcacttt attagcggct
     3481 gtgaccatcg caccgctaac ggaataaccc tcattaaagg tcgtaatacc attaaccgca
     3541 tcaataccat cagcaccgac aaacattaaa tcggcattaa tatcctgcaa tgaacgctcg
     3601 gcaatggaac catgcatcga gcgcgtttta tggcggaccg tgccaccaca gacgaccaga
     3661 gtaatgtctt tattttcgga aagggcaaac gctgccggga gactgttggt aatcaccgtg
     3721 atatttttag cggtcattaa tccttcggcg ataagcattg tggtacttcc gctgtcgaga
     3781 ataacggtca tcccttcatg gatcattttg actgccgcct gcgctattct gttttttggc
     3841 gcgctggcga gctgaaatcg ctctttaaac ccgacttcct gggtctcggt ttcactatta
     3901 ccggacatta ttttcgccgc accgccatga aagcgcgtaa ccacgccttt ttgttcgaga
     3961 aagcgcaaat cggcacggat tgtcgcttcc gaggcagcaa atactcccgc cagatcctga
     4021 acaagcacgg ttccctgttc attcactaat tggatgatct tattccttcg ctcgaatgaa
     4081 ttcatcgtca tttacccata ttcaattgta gctagtgtaa acgaaggggg accattggtg
     4141 aatgatctgt tttgcacaat accgggccag catcacgctg gcccgcggtt tcagggaatg
     4201 agcaacactt tgcccggcat ggcattacga gcgatgtcac gcaccgcctg ggcgaagctt
     4261 tcaaagcttc cacggtgagc gattaatggc tccaggctta acttacgttc tgtcagcaac
     4321 cggctcgccg tttcccactc ctgccccggc caagggctgg agtagttcat ccaactgccg
     4381 ataaccgtca gctctttacg caatattttg ccaaacgttg ccgatgttaa atgcagatcc
     4441 tgatgcaacg tgcccaccag cgccagttgg gcatgaggac cggcaatctc taccgccagt
     4501 tcgacagttt gcggtacgcc agccgtctcg aggataagct gattaaagcg cagttcgcgt
     4561 aaaacgctct gcatttgcgg cgcgctcatt tcgctactgt taaatgtttg catcgcaccg
     4621 aaagattttg ccagtgccag tttttctgag ctaatgtcga tagccgtcac actctttgct
     4681 cccagcgcga cagcgcactg aatagccagc aggccaatgg ttccggcacc aataataata
     4741 acgtttttat tctcacaacc ttgcgctaaa tgaaaagcat gcagtccaac ggtaatcggc
     4801 tcaataaaag ccccatcctc aataggcata tccgtgggta gagcaaagac attttttcgc
     4861 ttaacgacaa tatattcagc aaatccacca tcacgccgcg agccaataaa atcatatttt
     4921 gcgcactggg aataaaaccc tttcaaacac tctggacaag taaaacaggg taataacggc
     4981 acacaggcaa ccgcatcgcc agggtgtaaa tcatcaacac cggatcccac cgcatcaata
     5041 tagccgctaa attcatggcc taacgttatt ggataataat gtgcaccatt tttaaatatc
     5101 ctgggtaaat cggaaccaca taagcccgag ctggcaattt ttacccgtac ctcatcctga
     5161 tgtttaattt caggaatgac gctttctgca acgcgcacga taccatcagt atcattcacc
     5221 actgatttca taaaaactcc tgattgttaa ggggggataa actccccctc aggtaaaaat
     5281 tattctgcga gaacgacttt ctcttgttta ataaagccac gcgctctacg ccaggtcatg
     5341 aaaataccgg tcagataaat tgcaccgata ataatgaaac cgggaatatt ttgcggggag
     5401 aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga agccaccata
     5461 cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc aatagtttgc
     5521 gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt gcggaacaga
     5581 tttccacgat gcacggcgac cgccatcgcc acgaagaagc cgatggtggc aagatcgcca
     5641 aacggcagca cctgattacc cggcacacaa acagcaatta aaatggtgag tgggataaaa
     5701 atcaggcttg ccgataccac cgccgtatgt cccagcagca acgccggatc aaggccaatc
     5761 aggaactcct gaccgccgaa cttcgcctgt aaacgactac gagcctgctt agcgatgggc
     5821 gttaaaccat ccatgatggg tttaatcacc cgtggcatta gcagcatcac tgccgccgtt
     5881 tttaccgcca gctgcaatac acctttgaca tcgtaacccg cgaggatgcc gataatcagc
     5941 cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg ctgaatatcg
     6001 tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc atcgaccagc
     6061 accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc ttccagctca
     6121 aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga
     6181 attaccacac ctgccatccc tatcatccat gaaccggttg ccaggtgcag caacgcgccg
     6241 gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt catacgggtc
     6301 agtaacatcg ccacgttaac caatatcgca atcggaatcg ccaccagcgc aatttgcgac
     6361 gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag attcaggtcg
     6421 aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa gccaatcaca
     6481 aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca atcgcctgcc
     6541 ttcatgccta atattttaga aaaaataatg atgacaaccg gcagcatcac tgtagggccg
     6601 aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa gatagtcaga
     6661 attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc gtgaactaac
     6721 ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg cacaccatcc
     6781 atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat attatgattc
     6841 tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa
     6901 gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg gatttattcg
     6961 tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg aagagtgatt
     7021 agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat
     7081 tgctgctgcg gattttccac aatcaacgca ataaccaacg ataccgccac gtcgttatca
     7141 tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat ggctgacgac
     7201 ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag cattatcccg
     7261 gtagggaatt ctgcttctct ggcaattaat gcctgtggcc aggtatcatg aaccacacct
     7321 ttggcgagca tctcattacc gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt
     7381 ccgctacgaa caaacaggtt agtcatatac cgtccttatt ccgcacagcc gtagcgatag
     7441 gcgcgcaaaa catcataaat tttatccata atcagctgat gcggtattgc tgataattcc
     7501 ccggactgaa tgcgttcaaa ttgtttggga agatactgac taatcatgcc cagtgggatg
     7561 tccacgcctt caagattcac catcatcgtt tcgacgctat ttttaatccg actatgcggc
     7621 cagtaataac gaatacgatc cgacaggctg taacgaatat ccagtaatga atcgttaaaa
     7681 cccgtacgat aatatttttt ccagtattgc ggttcgtcga gcatcacttc ttcaattacc
     7741 gccaggcaac cgctgcgatt ttcaggggcg ataagttcct gctcaatttg tgccagtgca
     7801 aatatcgcct cgcgtaaagc aaaggttaat gcgggaccga ctttcaatat tgcaaagtga
     7861 tcgcggacta attcccaata agccgtccgg gtctggtaat cggtagaatg tgcttcataa
     7921 accattcggg tgttttctat ccattgcgcc agcggctgcg cttcctgcgg ctgataatgg
     7981 ataatattgc tgtgatcaaa ttccacgccc ggctgcacca cgatggcaat cacgcgtgtt
     8041 aacgcctctg tcagcccacg ggcaataaag gccttttgat gcgtacgtaa agtattggcg
     8101 gtatcttcaa catgggtgat gtgtactgac tgaatggcgc tggcctcacc gcccggaacc
     8161 ggaacttcgg tgccaatgac atagctcagt tgctcacgct ggcaatctgt cgccacactt
     8221 tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg tttctggtgc taacggtatg
     8281 ggatcccccg cgcaggacat tgacgcatca agatgaattt tactgaagcc ggcacgaaca
     8341 tattccttta ccagctcgac ggatttttcc atcgccgcat ccgcattttc ttgctgccag
     8401 cagtttggcc ccagatgatc gccgccgaga ataatgcgtt cgcgtgcaaa cccaacttta
     8461 tcggcaatcg taaaaacaaa ttcgcgaaag tctgccggtg tcattccggt ataaccgcca
     8521 aattgattga cctggtttga cgttgcttca atcagcactt tgcgcgtgct gttgcgatca
     8581 aatgccagcg ccgcttcgat aaccaacgga tgggcagaac agactgaaca tatgccgata
     8641 tgttcaccag ctttatgccg ggcaattaac gttttcatgt tttttcctta ttaaatggca
     8701 ggtgcgttat gccctgccct cgcagccaca atcggcaatc actttgctca ccacatcgcg
     8761 cattgcggat ttagccgact gcaaataatc ccggggatcg gtcgcttcag ggtgctcggt
     8821 caggtaattt tttaacgcct gcgagaaggc atttttcagc tccgttgcaa cgttgatttt
     8881 gcatatcccc agtttgatgg tttgctgaat atccttagtc gataaccctg acgcgccatg
     8941 cagcaccagc ggtaagttca cccactggcg aatgttctcc agtcgagaaa aatcaagcgc
     9001 cggtgcgctg gcatacatcc catgagccgt gccgatggcg accgccaggg aatcaattcc
     9061 ggttgcctcg gcaaattcac gcgcctgagc ggggttggta taaaaagcat cggcttcatt
     9121 gacttgcaca tcatcttcct ggccgccaag ttgccccagc tccgcttcga cgctgacatc
     9181 aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa atattttgcg caaaaggcaa
     9241 atgcgaggcg tcaatcatga ctgagcgcac gccagaacga accttctgag cgatatcgtc
     9301 aaatttcgtg tgatggtcga gatgaattgc cagtggatgg tgatattgct tcgccatcgc
     9361 gctgaccagc gccaacagat tttctgtacc agcatgagta aatgtgccag gcgttccggc
     9421 gatgatgacc ggcgcatgca ggttggcagc ggtttccacc accacttgca tcgtttcgag
     9481 attgtgaata ttgaatgccg gaaccgcata accgccgcgc tgtgcgttgt tcagcatctg
     9541 ctttgtcgat accacgtaca ttttcatatc ctgtcgtttg ttttcgattt caaaatataa
     9601 tgaaatgatt tgtttttaaa tgtcgagata acgatcacaa aaacgacaat gtgaaaattg
     9661 ttcgaggagt aaaaggcaaa aaaaaacggc ctcccgatag ggaagccgta gcaaagtgcg
     9721 cgtgttttta tgccggatgc ggtgtaaaca tacgtggcag tgctatgtag gcatgataag
     9781 acgcttccgc gtcgcatcag gcataggttg ccggatgcag tgtaaacgcc ttatccgtcc
     9841 tacggggtgg tgctgttcgt atgaggcgta tcaggcaata gtaattttgc tatcgagata
     9901 aacgtcctgc acggcattaa tcagtttcac gccgtcagcc atcgatttct tgaacgcctt
     9961 acgtccaaga atcagcccca ttccgccagc gcgtttgttg ataaccgcag tacgcactgc
    10021 atcactgagg tcagtttcac cgcctgcagc accgccggag ttaatcagcc ctgcgcgccc
    10081 catatagcag ttagctaact gataacgcac cagatcaatc gggttttcgc tggttaactt
    10141 gctgtacacg cgatcgtcgg tataaccgta attaattgct ttatagccgc cgttattttc
    10201 cgccattttt tgtttgacga tatctgcacc gatggttgcc gccagatggt ttgcctgacc
    10261 ggtcaggtcg gcggaaacat ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa
    10321 ataggcccac agcactgtca ccatacccag ctcgtgcgca cgttcaaaag ccgcagaaat
    10381 ttcttcaatc tggcgacgtg actcttccga gccaaaatag atagtcgcac caaccgcaac
    10441 cgcgcccatg ttgaacgcct gctccacgct ggcatacagc gtttgatcgt aggtattcgg
    10501 gtaacttagc gtctcgttgt gattaagttt gacgaggaat ggaatgcgat gcgcataacg
    10561 ccgcgatacc gacgccagca cgccgtaagt tgacgccaca cagttacagc ccgcttcgat
    10621 cgccagttca acaatgtttt tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc
    10681 ggcagagtgc tcaacgccct ggtcaaccgg cagaatagaa agatatcctg tgccagccag
    10741 acgcccggtg ttgtacaacg tctgcatatt acgtaacacc gctggcgggc gattattgtc
    10801 aatcattacg cggtctacgt agtcatgtcc ggggagataa agctggtcag aaggaattgt
    10861 catacaacgg tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg caatatctgt
    10921 cattactatg ctcccgtaaa ttccgattgg atatcggcta tggattgtcc tggcccgcct
    10981 tttgcgggca atcataatcc tggtcgttac gggcgagttt ttccatcttt tggatgtttt
    11041 ttcagcgttt tctgttggct cgattcatca gaaaaaatgt tagcgcggtc aagttttcac
    11101 cgcaaaggta tttaaaaggt attattaagt ggtattgtca tcgcgtacct tacattacct
    11161 gtcatgaagg aattaaaaga tgaaaacaac agcaaagctg tcgttcatga tgtttgttga
    11221 atggtttatc tggggcgcgt ggtttgtgcc attgtggttg tggttaagta aaagcggttt
    11281 tagtgccgga gaaattggct ggtcgtatgc ctgtaccgcc attgcggcga tcctgtcgcc
    11341 aattctggtt ggctccatca ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt
    11401 gatgttcgca ggcgcgctgc tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt
    11461 ctttccgtta ctgctggctt actctctaac ctatatgccg accattgcgc tgactaacag
    11521 catcgctttt gccaacgtgc cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg
    11581 cactatcggc tggattgcct ccggtctggc atgtggtttc ttgccgcaaa tactggggta
    11641 tgccgatatc tcaccgacta acatcccgct gctgattacc gccggaagtt ctgctctgct
    11701 cggtgtgttt gcgtttttcc tgcccgacac gccaccaaaa agcaccggca aaatggatat
    11761 taaagtcatg ctcggcctgg atgcgctgat cctgctgcgc gataaaaact tcctcgtctt
    11821 tttcttctgt tcattcctgt ttgcgatgcc actggcgttc tattacatct ttgccaacgg
    11881 ttatctgacc gaagtcggca tgaaaaatgc caccggctgg atgacgctcg gccagttctc
    11941 tgaaatattc tttatgctgg cattaccgtt tttcactaaa cgctttggta tcaaaaaggt
    12001 attattgctt ggtctggtca ccgctgcgat ccgctatggc ttctttattt acggtagtgc
    12061 ggatgaatat ttcacctacg cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta
    12121 cgatttttac tacgttaccg cttacatcta tgtcgataaa aaagcccccg tgcatatgcg
    12181 taccgccgcg caggggctga tcacgctctg ctgccagggc ttcggcagtt tgctcggcta
    12241 tcgtcttggc ggtgtgatga tggaaaagat gttcgcttat caggaaccgg taaacggact
    12301 gactttcaac tggtccggga tgtggacttt tggcgcggtg atgattgcca ttatcgccgt
    12361 gctgttcatg atttttttcc gcgaatccga caacgaaatt acggctatca aggtcgatga
    12421 tcgcgatatt gcgttgacac aaggggaagt taaatgaaaa cagaacgtat tctcggtgct
    12481 ctttatgggc aggcgttagg ggatgcgatg gggatgccct ccgagctttg gccacgcagc
    12541 cgcgttaaag cacactttgg ctggattgac cgttttcttc ctggaccaaa ggagaataac
    12601 gcggcctgtt attttaaccg cgccgaattc accgacgata cctcgatggc gctgtgtctg
    12661 gcggatgcgt tactggaacg tgaaggcaag atcgatccgg atctgattgg gcgtaatatt
    12721 ctcgactggg cgctgcgttt cgacgccttt aacaaaaacg tactaggtcc gacctcgaag
    12781 attgcgctta acgccattcg cgacggtaaa cccgttgctg aactggaaaa caacggcgtg
    12841 accaacggcg cagcgatgcg cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt
    12901 gattccttta ttgatgatgt agcgctggcg tccagcccga cccataaatc cgatctggcg
    12961 gttgcaggcg cggtagtcat cgcatgggcg atttctcgtg ccattgacgg agaaagctgg
    13021 tcagcgattg ttgattcact gccttcaatt gcgcgacatg cccagcaaaa acgcaccacc
    13081 acgttcagcg cctcactggc ggcgcggctg gagattgcgc tgaaaattgt gcgcaatgcc
    13141 gacggcaccg aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagcact
    13201 attgagtccg ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaatcgc
    13261 tgcgccgtcc tgtgcgctaa ccttggcggc gacacagaca ccatcggtgc tatggcgacg
    13321 gcaatttgcg gcgcgttgca tggcgttaac gctatcgatc ctgcgttaaa ggcggaactg
    13381 gatgcggtaa atcagcttga tttcaaccgc tatgccacag cgttggcgaa atatcgtcaa
    13441 caacgggagg tggtatgagc ggcgctcgat tacacacgct gctgcctgaa ttaaccacgc
    13501 gtcagccggt gatggtcgtc ggcgcggcgg tcattgacgt gatcgccgac gcttatgccc
    13561 tcccctggcg tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg
    13621 cactgaatat tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc
    13681 tcggtcaggg cgtgtgggcg gagattattc gcaaccggat ggcaaaagag ggcttaatca
    13741 gcctgatcga taacgccgaa ggtgataacg gctggtgtct ggcgctggtt gagccggatg
    13801 gcgaacgcac ttttatgtca ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg
    13861 cgcgattaac cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc
    13921 cctgcggcga attgttagtg gaatggctgg aagagctgca agacgtgacg ccgtttatcg
    13981 attttggccc acgtattggc gatatcccgg atgcattact ggcgcggatc atggcctgtc
    14041 gacctttagt gtcgctcaat cgtcaagagg ctgagattgc cgccgaacgt tttgctttat
    14101 ccgcagagat aacaacactt ggcgagcaat ggcaggagaa atttgccgcg ccgttgatcg
    14161 ttcgtctcga taaagaaggc gcatggtatt tcagcaacga cgcttctggc tgcattccgg
    14221 catttccaac gcaagttgta gacaccattg gggcgggcga cagtcatgca ggtggcgtac
    14281 ttgccgggct ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg
    14341 cgtcgtgggt tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc
    14401 tcgcacacaa aaacgtatag atcgctgcga cagtggctaa tgctgtactc aataggccgt
    14461 tgctgttggt caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga
    14521 atatgtgact gaaactcggc atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt
    14581 gggtaaatgt gctggctgcg gaaatagtca taaagtgaaa tcccgatggc atcaacatca
    14641 tgaattaaat gcgccggaac ccacgattcc tcaatcgata ccgcttcttc atcgacatag
    14701 cgaatacgct taagcaaaaa gacatcactt cctgcttcga ccgccagctg ctgcgcgact
    14761 tcttcgggac atttcacgac acgcttgtta acccatagcg tatcgggctt tttcccgcgc
    14821 aataccacct gctgagaaaa acctcgcgct tctttcaacg agtattcgaa gatgttgttg
    14881 atctgcgtgc cgtaaccgcg cgaacgcgtc accacaccct cttcttccag cgcctgcatc
    14941 gccttgcgca ccgtaatgcg cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc
    15001 aaaatattgc catgctccag caccccgctg cgcacggcat tttttaccgt ttcggcaaac
    15061 ttaatataaa gcggcgtgtt atccgccgct aaaatacgct cgttcagttg ggcaatcagc
    15121 tgggtatgcg cttgttccat ttgtcttttt cctgccgagg agtctggtgg gagtatatca
    15181 ctgcttctta tttaaggcgt ttctttaatc ccatcaacga atgcctgcaa ctcctccacc
    15241 gtgccatgaa atacattaaa atccaccgga ccattgatgc catctacctg tccacggtcg
    15301 gaatgctgcc agaagcgcca ggccatgccg tcattgtccg gacgacgttg ataatagtga
    15361 gccacccacc acggatattc attgaaatag cccgccagat tggtgtgata aaaaacggct
    15421 cctgagtaaa taatcggctt ttttcccgta cttttttcga ccatttttag ccactgactt
    15481 actcgtttgc gtaattcttt tgccgataat tttccccgtt cttctacgtc cagtaccgca
    15541 gggaaatcgc cttgtgagaa atccaccgtt tgcagaaata atcgtgcctg aaccggcgct
    15601 gctaccgacg gggagaaata atgatacgcc ccgcgcagca ggccattttc gcggcttagt
    15661 tgccagttac gcgaaaaata cgggtccacc agcttttcgc cttccgtcgc cttaataaaa
    15721 gcaaactgta agcggatacc gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc
    15781 tcctgccagc gcgaaacatc tattccgtga atggtataac tggcaggaat gcgaattgcg
    15841 aaagatttaa ccgggcgata actgtagaag tggatgtagt ctgtaatctg aactgctgtc
    15901 gaatagaaaa aattcaccgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga
    15961 gcgcataata gagaggtgag tttttttcga ctggtaattc ttagctgcat tggtttcatc
    16021 cctgaatgtc agtgccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca
    16081 ccaggcgtgg aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg
    16141 ggctaatgct gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc agtttgtatg
    16201 gcgcgggcgt agtgcagcca acgccgcaga gagcgtacaa ccagtgccgt gggtgttttt
    16261 ggtcataatg cgcggtgcgg taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg
    16321 gctttgctca tcatccagat gaccaccttt cattagcact gcgccacagc ccatcgccaa
    16381 cagcgatcgc ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa
    16441 ggcggcagct tcgggcaagt ttggcgttat taatgaaacc tgtggcaata atcgactgcg
    16501 cagcgtagca accgccgaag gtgaaagcag cgggtcgccg ctttttgcca gcataacggt
    16561 gtcgagtacc acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat
    16621 atcggtttcc gccaacatac cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga
    16681 atcgagctgg gcggcgacaa aatcaggctc aatgcgatac accgactgta cgccacgggt
    16741 attttgcgcc accagtgcag taataactga gcaaccataa gcgccaagtg ccgagaaggt
    16801 tttaagatcg gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc
    16861 gttaattcgt ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt
    16921 ggaacaaaac tgcctggccc ctcgcttctg gcgactgcgc gctctccggc ttgtttcatc
    16981 cagtgacagg cagatgcgac attttccagc gtatcgcctg gtaacgcaca gcaggtagcg
    17041 acaaccgccg ataatgcaca gccagttcct accactttgg tcattaacgg atcgccaccg
    17101 tgaataccaa tgatacgatg tccatcggta acataatcca tctcgccagt gaccacgacg
    17161 attgcgccag tttcccgtgc cagtgtttgt gcagcgggta tcgcgttagc tgcggcgtca
    17221 gtggtatcca ctccccgtcc accattagca atgccagcta atgccatgat ttccgaagca
    17281 ttaccacgta tggcggttgg cttatgagac agaagttcca gacaaaaacg gcggcgataa
    17341 tcgagtgcac caaccgctac tggatcaagc gtccagggtg tttgagagct ttttgcttgc
    17401 tcaacggcgg cacacatcga ctgtgcgcgt agctgcgtca gcgtgccaac gttaatcagc
    17461 aacgcactgg caatagccgc aaactgactg gcctcttcgg tttcgataac catcgctggc
    17521 gatgcaccga gcgccagcaa ggtattggca gtaaaggttt gcaccacatc gttggtcatg
    17581 cagtgcacaa gaggggaatg ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa
    17641 ctcagcaggt cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt
    17701 ggtccgtgac ttccctacgc tggcattatc cagatcaggt gatacgggta tttctcagcc
    17761 ttcacgcaga agggcacccc gagtcgtttg gttgcgatga taaatcgcag aggaggatgg
    17821 taatgtccag cgcacgcgtt gtaaacgagg aaaaacggca gttttacaat cgcgttaata
    17881 aattatttga tatatgaatc cagcaccttc agaacgacat ccagatcttc ttcacgtttt
    17941 agctcatccc cctggtgaac gatgtgttcc gtcagatgac ctttaatcac ttcccgtatc
    18001 agaccgttta ccgcgccacg gatagcagca atctgttgta aaactgcagc gcattcgtgc
    18061 ggctcgtcga gcattttctt gagcgccacg acctggccct gaatcttact ggcacgcgct
    18121 ttcagtttct gtttatcacg gattgtatga gacatggcaa cacctggtta acaagaatat
    18181 gaaaaatcat agcactatta atctactggg gg
//
LOCUS       DAFAVF010000080        18053 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFAVF010000080 DAFAVF010000000
VERSION     DAFAVF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.080"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            5..511
                     /locus_tag="LGK42_002746"
     CDS             5..511
                     /locus_tag="LGK42_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBK2658975.1"
                     /translation="MTCISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAER
                     LLAGLPAH"
     gene            complement(521..1948)
                     /locus_tag="LGK42_002747"
     CDS             complement(521..1948)
                     /locus_tag="LGK42_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBK2658976.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1945..2652)
                     /locus_tag="LGK42_002748"
     CDS             complement(1945..2652)
                     /locus_tag="LGK42_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBK2658977.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2816..3793
                     /locus_tag="LGK42_002749"
     CDS             2816..3793
                     /locus_tag="LGK42_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBK2658978.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLSAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3863..4345)
                     /locus_tag="LGK42_002750"
     CDS             complement(3863..4345)
                     /locus_tag="LGK42_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBK2658979.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4580..7162
                     /gene="leuS"
                     /locus_tag="LGK42_002751"
     CDS             4580..7162
                     /gene="leuS"
                     /locus_tag="LGK42_002751"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK2658980.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7177..7758
                     /gene="lptE"
                     /locus_tag="LGK42_002752"
     CDS             7177..7758
                     /gene="lptE"
                     /locus_tag="LGK42_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBK2658981.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7758..8789
                     /gene="holA"
                     /locus_tag="LGK42_002753"
     CDS             7758..8789
                     /gene="holA"
                     /locus_tag="LGK42_002753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK2658982.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8791..9432
                     /gene="nadD"
                     /locus_tag="LGK42_002754"
     CDS             8791..9432
                     /gene="nadD"
                     /locus_tag="LGK42_002754"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK2658983.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9456..10067
                     /locus_tag="LGK42_002755"
     CDS             9456..10067
                     /locus_tag="LGK42_002755"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBK2658984.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10327..10644
                     /gene="rsfS"
                     /locus_tag="LGK42_002756"
                     /gene_synonym="rsfA"
     CDS             10327..10644
                     /gene="rsfS"
                     /locus_tag="LGK42_002756"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK2658985.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10648..11115
                     /gene="rlmH"
                     /locus_tag="LGK42_002757"
     CDS             10648..11115
                     /gene="rlmH"
                     /locus_tag="LGK42_002757"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK2658986.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11146..13047
                     /gene="mrdA"
                     /locus_tag="LGK42_002758"
     CDS             11146..13047
                     /gene="mrdA"
                     /locus_tag="LGK42_002758"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBK2658987.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13050..14162
                     /gene="mrdB"
                     /locus_tag="LGK42_002759"
                     /gene_synonym="rodA"
     CDS             13050..14162
                     /gene="mrdB"
                     /locus_tag="LGK42_002759"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBK2658988.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14173..15261
                     /gene="rlpA"
                     /locus_tag="LGK42_002760"
     CDS             14173..15261
                     /gene="rlpA"
                     /locus_tag="LGK42_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBK2658989.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15401..16612
                     /gene="dacA"
                     /locus_tag="LGK42_002761"
     CDS             15401..16612
                     /gene="dacA"
                     /locus_tag="LGK42_002761"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBK2658990.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16723..16986
                     /locus_tag="LGK42_002762"
     CDS             16723..16986
                     /locus_tag="LGK42_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBK2658991.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17087..17728
                     /gene="lipB"
                     /locus_tag="LGK42_002763"
     CDS             17087..17728
                     /gene="lipB"
                     /locus_tag="LGK42_002763"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBK2658992.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4588 a         4523 c         4590 g         4352 t
ORIGIN      
        1 agcaattacc tgtattagcc aacccaagaa aacaacgttg cgtgataaag ccatggtgaa
       61 ttatgccttt gattatttaa gctcaccggg cagtctaccg tttaccacgg cggccacgga
      121 gctttccgcg attcatggtc actcaacgtc gcaatatcgc cttggagaat tttatcttca
      181 tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg agcaatcagc
      241 agaacaggaa aatccgcgcg cacaaagcaa actggggtgg atctacctca aaggtctggg
      301 ggtcaaaccc gacacccgta aagcaattct ctggtataag gaagcagctg aacaagggta
      361 tgctcatgct caatatactt taggtttgat ctacagaaat ggctcaggta ttaatgttaa
      421 ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca aaaatgcgga
      481 aagattactt gccgggcttc ccgcacatta acgtcgactg tcaaccgaac agatggagta
      541 ttttccctat caaaccagca aagataaaaa tatagaaaat aattttaatg atgcccagac
      601 agcctggccc accatcgtca acgggcattg gctgcggttg cggctccggt tcaacagttt
      661 gcttctcggt aaaattcgcc gcctgaatta ataatcgacg aataattaat gcttccagtg
      721 gacaacgatc ttctgcatca aggcggctca caatcggcat taatgattct tcggttggtt
      781 ggttcgcctg cactaattgt ttggcaacta aatataatgg ccataaggga tgttctgtat
      841 cacgcgtgag tgagatccgc ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac
      901 gccccagagc agagagtttt tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg
      961 cgctgtcgcc ggggatataa agtagcggtt ttccctgatc gttaaccagt tcacgagctt
     1021 caatcatcat caccactaac gggtaataat cgacccgatg ctgacggcac cattgcaata
     1081 ttaaagcatc cgcctgggtg gagggatact cgcgccagta gtcacaaagt tctgccagca
     1141 agctttcgcc ttctccacaa tagacgcgtt gtgcgtattc ataataacgc gcatcttcat
     1201 tatcagattc agcttcccgc cacgaccggg cgtaatccag gagttcggga attcccaggc
     1261 gacctgcgct ataccagcgg agtaaatttc tgtgcagttt tgcatcatcg gggataatca
     1321 ctgcgcgatg ttgcgctaaa tacaatgtca gccattctgg atgcgatgac caaatccgag
     1381 cgaacatttg gtaaaaatcg atggtgtcat tttgcacggc aacgggcaga tgcagaatgg
     1441 tctggaagtt aaaaacattc cccttcgcaa tggcatataa aaaggattcc agttcttctt
     1501 cgtctatttc cccgacgttt tcctcctgca accaccgcaa tctttctgct aataacacca
     1561 cgcactcgaa ggataaatgg gcagtgttca tggcgattgt gcacagcgac cagcgtaatt
     1621 catcaatctc ttccatcgag caataattta attgctgaat gaatcgctgc caggcggagg
     1681 gcagaaaacg ttcgctatca gaggcaagta acgcacgaaa ggcgagcaga atttcatgtt
     1741 ctgctacctc atattcttct ggttgccaaa cagatttagc aggcgactgc gcaatccgta
     1801 gcgcctcctc atacgcctga cgaagttgtt taaaaccctg cggatcggtt tctggatgaa
     1861 aggacggtaa cagcgccaga taagcgcggc ggataatatc gacgtcagtc gtttcctcta
     1921 tttcgagaat cttccagcaa tttttcatag gtcgctcata tccttaagcg tttccgggca
     1981 tgcagaatag taagccaatg gttgccagga caaggtaggg taatacgcat catcactggc
     2041 aaataattga taaaacatat cattgccgga atcgtcgtat tctttacgcg ctaagaattc
     2101 gcgtaataac tgcagatgat catcttcggg agactgccaa tattgccgac caaaggtata
     2161 accggcaaag tattgccgcc agttacgata ataacgtaga gcccgtagat gaatgcgcga
     2221 ctggatccat agactttctt cttcgctcaa ccagttatta agtacgccca ttcggcttaa
     2281 aaaacccatc cggacataat cccaggcttt aattcctccc tcaccacagc attcggcggt
     2341 gctggcgaca aattgcgcat aagcctgacc ttgctcattt agttcagcaa gatagtcacg
     2401 ccattcacat ggcgaataac gaaaccagcg gtgataaaac cctgccaacc gagcggcatg
     2461 accgttatcg atcattcggt gaattgtgcg cagcaggtca tcacgggcat caattcccca
     2521 ggaggaagaa aggtcaaaaa agcatgtacc taaatacatt ttgggtgatg aatgcgaagg
     2581 taaacagcat tcattgcgta tttccatcgg agtggaaagt gcaaacaata ttttttgcga
     2641 ctccatgtcc atataattta tccttaaata ttcattaaat gaatattagc cgcattgcga
     2701 ggctcgttta tttatacttt tctgacgcga cataatacgc ataaaataag gcttacgcaa
     2761 aaatggaaca cataaaaata taatatgttt tatctgcaac cataaggaaa tgataatgat
     2821 tttcacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
     2881 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
     2941 ttctccggac gacgaaaaag ccttttactg gctgaagctg tccgctgagc aaggtcattg
     3001 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
     3061 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
     3121 cgcccttggc tggacactgg atcgtggaga agcccccaat tataaagaag cggttgtctg
     3181 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta
     3241 cagaaatggc aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc
     3301 tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa
     3361 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
     3421 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
     3481 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
     3541 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
     3601 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
     3661 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
     3721 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     3781 agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt
     3841 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac
     3901 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     3961 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     4021 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgata tctgccagct
     4081 cctgccataa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     4141 tctcttcata gctcatggcg aactcttcca ggtcacgtct tacagctcgc gtcagctcat
     4201 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     4261 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac
     4321 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     4381 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
     4441 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     4501 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     4561 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     4621 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     4681 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     4741 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
     4801 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     4861 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     4921 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     4981 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
     5041 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     5101 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     5161 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     5221 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     5281 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     5341 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     5401 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     5461 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     5521 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     5581 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     5641 gaacatcaaa ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc
     5701 cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc
     5761 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     5821 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
     5881 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
     5941 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     6001 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     6061 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     6121 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     6181 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     6241 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     6301 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     6361 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     6421 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     6481 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     6541 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     6601 gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     6661 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
     6721 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     6781 caaactggcg aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct
     6841 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
     6901 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     6961 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     7021 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     7081 ggtagcaaaa tatcttgatg gcgttactgt gcgtaaagtg atttacgtac caggtaaact
     7141 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
     7201 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
     7261 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     7321 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     7381 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     7441 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
     7501 ttgatccccg gccgtgatat ctacccgatt agtgccaaag tcttccgttc gttctttgat
     7561 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     7621 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     7681 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     7741 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     7801 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     7861 aggacgctgt tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca
     7921 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     7981 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     8041 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta
     8101 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
     8161 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg
     8221 caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc tactgttatg
     8281 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
     8341 gcaaattgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     8401 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     8461 aactgcgtct ggaaggcagc gagccggtta ttttgttgcg cacattacaa cgtgaactgt
     8521 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     8581 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagcc
     8641 agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     8701 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     8761 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
     8821 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
     8881 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     8941 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     9001 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     9061 gagtggcggc aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca
     9121 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     9181 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     9241 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     9301 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     9361 ggtgaatcgt gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc
     9421 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     9481 gcgaaacgca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg
     9541 cacgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt tcctttgatc
     9601 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc
     9661 gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     9721 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     9781 actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     9841 aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta gtcgtcagcc
     9901 atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc gaggctatgt
     9961 ggcattttcg cgttgaccag gggtgttgga gcaccattga tatcaaccaa aaattcgcga
    10021 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
    10081 ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc tccgcagcca
    10141 gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
    10201 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
    10261 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
    10321 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
    10381 ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc
    10441 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
    10501 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
    10561 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
    10621 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
    10681 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
    10741 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
    10801 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
    10861 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
    10921 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
    10981 tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
    11041 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
    11101 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
    11161 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
    11221 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
    11281 cgctttaccg actaccagac tcgctctaat gaaaaccgca ttaagctggt gcctatcgcg
    11341 cccagccgcg gcattatcta cgaccgtaat ggtatccctc tggccctcaa ccgcactatc
    11401 taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct ggacgctttg
    11461 cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
    11521 tcacaccgtt tcacctctat tccggtgaaa actaacctga ccgaagtaca agtagctcgc
    11581 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
    11641 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
    11701 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
    11761 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
    11821 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
    11881 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
    11941 ctggcgggta gccgcgcagc tgtggttgtc accgatccgc gtacaggtgg ggtgctggcg
    12001 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
    12061 tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
    12121 tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg
    12181 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg ttcggaaaaa
    12241 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
    12301 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
    12361 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
    12421 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
    12481 ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg
    12541 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttcctca tttgctgatg
    12601 agcaccgccg aagacggcaa acaggtgcca tgggtacagc cgcatgaacc gcccgtcggc
    12661 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
    12721 cctaacggta cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatcc
    12781 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    12841 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    12901 gctgtcgcca tgattctgga gaacggtggt gcgggtccgg cggttgggac actgatgcgc
    12961 cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca
    13021 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    13081 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    13141 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg
    13201 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    13261 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    13321 ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt gttcgttttc
    13381 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    13441 acgtttgccc gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca
    13501 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    13561 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    13621 tggcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    13681 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    13741 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat ggcactcagt
    13801 cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    13861 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    13921 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa
    13981 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    14041 ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt gtgctgatgg
    14101 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    14161 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    14221 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    14281 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    14341 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    14401 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    14461 tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct tccgatcccc
    14521 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    14581 cgcggcccct acggcaacga ccgcgtaatt tcgctttctc gcgcagcagc tgaccgtctt
    14641 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    14701 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    14761 cctcccgatt taagcggtgg cgcgggggca agttcagtgt ctggcccgca gggtgacatt
    14821 cttccggtca gtaactcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    14881 agcggtttcc tcggcgctcc aacgacctta gcgcctggtg tactggaaag cagcgaaccg
    14941 acacctgcac cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca
    15001 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    15061 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    15121 gtccccggtc gcgtaactca aaatggcgcg gtctggcgta tccagcttgg cccattcgcc
    15181 agtaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    15241 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    15301 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    15361 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    15421 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    15481 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    15541 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    15601 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    15661 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    15721 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    15781 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gcgtcgccat
    15841 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    15901 cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg ctgatggtca
    15961 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    16021 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    16081 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    16141 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    16201 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    16261 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    16321 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    16381 gaccattcca cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    16441 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    16501 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    16561 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa
    16621 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    16681 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    16741 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    16801 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    16861 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    16921 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    16981 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    17041 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    17101 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    17161 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    17221 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    17281 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    17341 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    17401 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    17461 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    17521 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    17581 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    17641 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    17701 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    17761 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    17821 tcacttgcag aaatgcggtg ccacgccttg ctccgcaact caccccattg attaacctat
    17881 tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt
    17941 aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa
    18001 tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tat
//
LOCUS       DAFAVF010000081        17914 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFAVF010000081 DAFAVF010000000
VERSION     DAFAVF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.081"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            10..4860
                     /gene="ehaG"
                     /locus_tag="LGK42_002764"
     CDS             10..4860
                     /gene="ehaG"
                     /locus_tag="LGK42_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HBK2658993.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSTDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            5228..6883
                     /gene="lldP"
                     /locus_tag="LGK42_002765"
     CDS             5228..6883
                     /gene="lldP"
                     /locus_tag="LGK42_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBK2658994.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6883..7659
                     /gene="lldR"
                     /locus_tag="LGK42_002766"
     CDS             6883..7659
                     /gene="lldR"
                     /locus_tag="LGK42_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBK2658995.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MTHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            7656..8846
                     /gene="lldD"
                     /locus_tag="LGK42_002767"
     CDS             7656..8846
                     /gene="lldD"
                     /locus_tag="LGK42_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBK2658996.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9000..9473
                     /gene="trmL"
                     /locus_tag="LGK42_002768"
     CDS             9000..9473
                     /gene="trmL"
                     /locus_tag="LGK42_002768"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK2658997.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9526..10347)
                     /gene="cysE"
                     /locus_tag="LGK42_002769"
     CDS             complement(9526..10347)
                     /gene="cysE"
                     /locus_tag="LGK42_002769"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK2658998.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10427..11446)
                     /gene="gpsA"
                     /locus_tag="LGK42_002770"
     CDS             complement(10427..11446)
                     /gene="gpsA"
                     /locus_tag="LGK42_002770"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK2658999.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11446..11913)
                     /gene="secB"
                     /locus_tag="LGK42_002771"
     CDS             complement(11446..11913)
                     /gene="secB"
                     /locus_tag="LGK42_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBK2659000.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11976..12227)
                     /gene="grxC"
                     /locus_tag="LGK42_002772"
     CDS             complement(11976..12227)
                     /gene="grxC"
                     /locus_tag="LGK42_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBK2659001.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12368..12799)
                     /locus_tag="LGK42_002773"
     CDS             complement(12368..12799)
                     /locus_tag="LGK42_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK2659002.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGLQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13044..14588
                     /gene="gpmM"
                     /locus_tag="LGK42_002774"
                     /gene_synonym="pgmI"
     CDS             13044..14588
                     /gene="gpmM"
                     /locus_tag="LGK42_002774"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBK2659003.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14598..15881
                     /gene="envC"
                     /locus_tag="LGK42_002775"
     CDS             14598..15881
                     /gene="envC"
                     /locus_tag="LGK42_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBK2659004.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15885..16844
                     /locus_tag="LGK42_002776"
     CDS             15885..16844
                     /locus_tag="LGK42_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBK2659005.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(16831..17865)
                     /gene="waaH"
                     /locus_tag="LGK42_002777"
     CDS             complement(16831..17865)
                     /gene="waaH"
                     /locus_tag="LGK42_002777"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBK2659006.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4424 a         4671 c         4719 g         4100 t
ORIGIN      
        1 gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact
       61 gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt
      121 tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat
      181 gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg ggttgctata
      241 ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac tgctgtgggt
      301 tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt
      361 ggcggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag aagtatagca
      421 ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg ttattcaaaa
      481 gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg agcaaacgcc
      541 attgccctgg gaaatgccac taaagctact gagattatga gtattgctct tggcgacacc
      601 gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa gtagcgtcgc atctgaagaa
      661 aacgcaattg ccctggggcg tagcagtgta gctagcggta ctgacagcct cgcatttggc
      721 agacaatcac ttgccagcgc agcgaacgct attgcgatag gtgctgagac cgaagccgct
      781 gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag ggactaatag catggcaatg
      841 gggttcggaa gccttgccga taaagtcaat actatcgcat taggaaatgg cagccaggct
      901 ctggcagata atgcaatcgc cataggccag ggcaacaaag ctgatggcgt ggatgccatc
      961 gctctgggta atggtagcca gtcgagaggc ttaaacacca ttgccttagg cacagccagt
     1021 aatgcaactg gtgataagag tcttgcgctt ggtagtaata gcagtgccaa cggtattaac
     1081 tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca ataccgtctc tgtcggcaat
     1141 agttcattaa aacgcaagat cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac
     1201 gatgccatta atggttcaca gctttatgcc attagcgact cggtagcaaa aaggcttgga
     1261 ggaggggctg cagtagatgt tgatgacggt actgttacag caccaaccta caatttaaaa
     1321 aatggtagca aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa
     1381 tgggaccaaa ccaaaggcaa atacagcgct gctcatggta ctagtagccc aactgccagc
     1441 gtaatcaccg atgttgcgga tggcacgatt tcagcctcca gtaaggatgc ggttaacggt
     1501 tcccaactga aagctaccaa tgacgatgtc gaagccaata ccgccaatat cgctactaat
     1561 accagcaaca ttgccacgaa tacggcaaat attgccacca ataccaccaa tatcaccaac
     1621 ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga aactaaaaag
     1681 gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt taaagatgcc
     1741 gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac caccaacgat
     1801 gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc cactaacacc
     1861 accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     1921 gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag caaaatcacc
     1981 aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg ctctcagctg
     2041 aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa caccaccaac
     2101 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     2161 gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc
     2221 ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac
     2281 ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg
     2341 tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg
     2401 gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc
     2461 gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc
     2521 gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc
     2581 gtgagcagtt atgttgttga tgcgctggga ggtggtgccg aagtcaatgc agacggcacc
     2641 atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg
     2701 aatgctatcg ataccactct tgacgacgct ctgctctggg atgcggacgc cggtgaaaat
     2761 ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct
     2821 aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc
     2881 aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc
     2941 accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat
     3001 gcgcttgatg ataacgcctt gctgtgggat gagactgcca atggcggtgc tggagcctac
     3061 aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt
     3121 gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag
     3181 cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatccaagaa
     3241 aacggtgcgg gtattaacta tgtgcgtact aacgacgacg gcttagcgtt caacgacgcc
     3301 agcgcacagg gtgttggcgc tacagctata ggttataact ctgtcgccaa aggcgatagc
     3361 agcgtagcta ttggtcaggg cagctacagc gacgttgata cgggtatcgc cctgggtagc
     3421 agctctgttt ccagccgagt gattgcaaaa ggctcccgtg acaccagcat aacggaaaat
     3481 ggcgttgtta ttggttacga caccacggat ggcgaactgc tcggtgcatt gtctatcggt
     3541 gatgacggta aatatcgtca aatcatcaac gtagccgatg gttccgaagc ccatgacgcc
     3601 gttacggttc gtcaattgca gaatgcgatt ggtgcggtcg caaccacgcc gactaaatac
     3661 ttccacgcta attcaacgga agaagattca ctggcagtgg gaactgactc gctggcaatg
     3721 ggtgcgaaaa ccatcgtgaa tggcgataaa ggtattggta tcggttatgg tgcctacgtg
     3781 gacgcgaatg cacttaacgg cattgccatt ggtagcaatg cgcaagtcat tcatgtcaac
     3841 agtattgcga taggtaatgg ttctacgacc actcgtggcg ctcaaaccaa ttataccgcc
     3901 tacaacatgg acgcaccgca gaactctgtc ggtgaattct cagtcggtag tacggatggt
     3961 caacgtcaga tcactaacgt cgcagcaggt tcggctgata ccgatgcggt caacgtgggt
     4021 cagttgaaag taacggatgc gcaggtttcc cagaataccc agagcattac taacctgaac
     4081 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgatatc
     4141 gtcaccaccg gtagcaccaa gtacttcaag accaataccg atggtgtaga tgccagcgcg
     4201 cagggtaaag atagcgtcgc gattggttct ggctccattg ctgccgcgga taacagcgtc
     4261 gcactgggta cagggtctgt ggcaaccgaa gaaaatacga tctctgtagg ctcatctact
     4321 aatcaacgtc gtatcaccaa cgtagccgca ggtaaaaacg acaccgatgc tgttaacgtg
     4381 gcacagttga agtcttccga agctggcggt gtgcgttacg acaccaaagc tgatggttct
     4441 atcgactata gcaatatcac cctcggtggc ggcaacggcg gtacgactcg tatcagcaac
     4501 gtctccgctg gcgtcaacaa caacgacgcg gtgaattacg cgcagttgaa gcaaagcgtg
     4561 caggaaacga agcaatacac cgatcagcga atggttgaga tggataataa actgtctaaa
     4621 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag
     4681 gcttacactc caggtgccag catggcctct attggtggcg gtacttacaa cggtgaatcg
     4741 gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa
     4801 ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa
     4861 tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca
     4921 tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga tgaggattgc
     4981 ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc ttcgcttatc
     5041 tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa tacataacat
     5101 ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc cacttttaca
     5161 caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg actccaggag
     5221 acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct ggctttccag
     5281 tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc tcaaactgaa
     5341 aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt tgctgttcta
     5401 taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct atggtttgtg
     5461 gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg
     5521 gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc gtctgcaaat
     5581 gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc
     5641 ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg
     5701 actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca ttccaatcct
     5761 ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg tggggcggca
     5821 gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg acggctggcg
     5881 cggtatcaag gagacgtggc ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca
     5941 gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt cgctggtatc
     6001 actgctctgc ctgacgctgt tcctcaaacg ctggcagcca gtgcgcgtat tccgttttgg
     6061 tgatttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca ctgcgggtca
     6121 ggtgttacgt gcctggacac cgttcctgtt cctgacagct accgtaacac tgtggagtat
     6181 cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat
     6241 tccggtgccg tacctcgata aactggttgc ccgtatgcca ccagtggtca gcgaggctac
     6301 agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg ccattctgtt
     6361 tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta tcagcacctt
     6421 cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg tgctggcgtt
     6481 cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac tggcgcacac
     6541 cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg
     6601 gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat
     6661 tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca ccggtaagat
     6721 gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc
     6781 tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg tgggcgtgat
     6841 caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt tttacccaga
     6901 cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa
     6961 gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat
     7021 tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc
     7081 ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat cgtccagccg
     7141 ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga agcccgctac
     7201 gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa
     7261 aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc ctcacaagcg
     7321 gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct gctgcaaacc
     7381 atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca gcggatgtat
     7441 ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat tgacgccatt
     7501 tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatga cgcaccttag ttttgttcac
     7561 accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac ccgcctgccc
     7621 ggtgagcatt atgagcattc gagggagaaa aacgcatgat tatttccgca gccagcgatt
     7681 atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg gatggtggtg
     7741 catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc
     7801 agcgtattct gaaaaacatg tctgacttaa gcctggaaac gacgctgttt aatgagaaat
     7861 tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg
     7921 aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc tcgacggttt
     7981 ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt
     8041 atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt
     8101 gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc
     8161 attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc
     8221 cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt aatatctcag
     8281 cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg aataacttcg
     8341 atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat ggcccgatgg
     8401 tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg
     8461 gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct tccgctcgtg
     8521 cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg gatagcggaa
     8581 ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc gtacttctgg
     8641 gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct aacctgctaa
     8701 atctgatcga gaaagagatg aaagtggcaa tgacgctgac tggcgcgaaa tcgatcagcg
     8761 aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctgcg gcactggccc
     8821 cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tccccctaac cctctccctc
     8881 agggagctgt ctcttgatca gatctcctga tcaagagaca gggggaggcc ggggtgaggg
     8941 gaaaaggccg cactgctcac caattttctg ctatcctgcc cccgcactaa gggggcagta
     9001 tgctaaacat cgtactttac gaaccagaaa ttccgccaaa tactggcaac atcatccgtc
     9061 tttgcgctaa taccggcttt cgtctgcata tcatcgaacc gatgggattt gcctgggacg
     9121 ataagcgcct gcgccgcgcg gggctggact atcacgagtt taccgccgtt acgcgtcatc
     9181 atgactttcg cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc ctcaccacga
     9241 aaggtacgcc tgctcacagc gccgtaagct atcaggatgg cgattatctg atgttcggcc
     9301 cggaaacacg cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc
     9361 gcattccgat ggtgccggac agccgcagca tgaacctgtc caatgcggtg tcagttgtgg
     9421 tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt gttgagagat tagttactgt
     9481 atgccggata gatgattaca tcgcatccgg cacgatcaca ggacattaga tcccatcccc
     9541 atactcaaat gtatggttaa taccgttgaa atgctggtcc atatccattg atggcttatc
     9601 gctgtctggt ttaccgacaa tacgagccgg aacgccagcg gcggttgtat gcggcggcac
     9661 cggttgcagc accacggaac ctgcgccaat cttcgcgccg cgaccaactt caatattgcc
     9721 gaggattttc gcgcccgcgc caatcatcac accttcacga attttcgggt gacggtcacc
     9781 accagattta cccgtaccgc caagcgtcac agattgcaga atcgatacgt cgttttcaat
     9841 taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg agcataatgc cacggccaat
     9901 ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa acctggtttt gcagaaagat
     9961 tgccaatgcg cgacgcccct gattccacaa ccagtgaccg atgcgatagg cctgcaaggc
    10021 atgaaaaccc ttcaggtata acaacggggt tgagtattta tcgactgccg ggtcgcgggt
    10081 acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt tccgggtcag cggcgtaggc
    10141 ttcttccacc acttcacgga tagcaatagc aggcataatt ggcgatgaca gcttgttcgc
    10201 cagcatgtag ctcagtgcac ttccaaggtt ttcgtgcttg agtagcgtcg cgtggtaaaa
    10261 actggccagc attggctcac agtccgccag cgttctggct tcggctttaa tattgttcca
    10321 gacaatttcc agttcttcac acgacattgc ttactccaca cgatgagata atgaccggcc
    10381 cgttctgcgc gggccgggtc atagcggtaa caaaggttcc ctggggttag tggctgctgc
    10441 gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc ctcgcgcgcg ttttttccgc
    10501 aatataatac ttgataaatt tcctcggtta ttggcatttc aacgccgaag cgatgcgcca
    10561 gttcgcggac ttctttcgta ttgcggtagc cttccaccac ctgaccaatc ttctcctgcg
    10621 cgctttgtac atccatgccc tgaccgagca tcatgccaaa acggcggtta cgcgactggt
    10681 tgtcggtaca ggtaagcacc agatcgccaa gccccgccat gcccataaag gtggcaggat
    10741 cggcacccag cgccgcacca agacgcgaca tttcagccag cccacgggtg atcagcgccg
    10801 tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc cgcgccaatg gcaatgacgt
    10861 ttttcaccgc accgccaagc tgcacaccga tgaaatccgg gttgctgtaa acgcggaaac
    10921 ttttgccaca gtgcagcaat tgttggagat catcggcaaa ggtctggtcg gtagaggcca
    10981 acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc aaacgttggg ccagagataa
    11041 ccgccagcgg aatttgatcg cctaaggcct cacgcgccac gtcctgtaac agacgtccgg
    11101 tttccgcttc cagccctttg gtcgcccaca ccagacgcgc atcaggacgc atcagcggtt
    11161 taatctggcg cagcacttca ccaaagacat ggctgggtac gacgacgaga atattacggc
    11221 tggctgccag cgcagtggcg agatcgcttt caagatggag cgtatcggga aaaggcacat
    11281 cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt tcagggtcat
    11341 ggccccagag gacaacctcg tggccatttc ttgccagggt gatggcaaga gcggtgccgt
    11401 acgagccggc accgatcaca gtcattgaag cattacgttg gttcatcagg catcctgatg
    11461 ttcttcagta ccttcgccag cctgctgctg caaatagttc atgaacagcg catcgaagtt
    11521 aaccggcgca aggttcagtt gcgggaatgt accgcgggat accatgctgg tgatgcactc
    11581 acgagcatac gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg ccatctgggt
    11641 gccttcgata cccgcgatgg agaaaatacc gccctgctga acttcacaca ggaacgcggt
    11701 ttcttcgccc agggaggccg ttacggtaac acgcagtacc acttcgtaaa cgtcatctgc
    11761 cagttgagta gatgccgtat ccagatcaag tttaacttct ggctgccaat ctttctggaa
    11821 aacgtgcggc gcgttcggcg cttcgaaaga aatatccttg gtataaatac gttggatctg
    11881 gaaagtcatt tcggtgttgt tttgttctga catgtgtaga aaacccttaa gtgttgtcct
    11941 taaatactgc gcaatgtaat gccaacgttc acacgttatt tcagcagggg atccagtcca
    12001 ccacgtgcat ccaatgcata caagtcatca cagccgccaa tgtgctgtgc gtcaataaaa
    12061 atctggggaa ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt ggcggcattg
    12121 ccatcgatcg gcaactcctg gaaactcacg cccttgctgc tcagcagtgc ttttgcacga
    12181 tggcaatacg ggcaggtttc tttagtatag atttcaacat tggccataaa ttagctcctg
    12241 ttttttaccc tgaaatttca tgttgcaggg cggcagcaaa atggagtgtc cccagaagct
    12301 tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac
    12361 aggtaaatta tttgccgcgc accaaaggca agttttcgcc agcccagcca gcgacgccct
    12421 ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcatttgca ggctcctggc
    12481 actgcaggcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac
    12541 caacattgtt ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt
    12601 tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac
    12661 gcgtagcttc accacgacta atcaccttca ctttcgaggt caggctctta aacgtagtca
    12721 caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa
    12781 attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg
    12841 aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt
    12901 tgcggggcgc gacgaaaaaa aagcgtaaat ctggcatcgg cttgccgcga gcggtcgtat
    12961 attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga
    13021 ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc
    13081 tggatggcta tggctatcgc gaagaacagc aggataacgc catttttagt gctaaaaccc
    13141 cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg
    13201 aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg
    13261 ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt
    13321 tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag
    13381 tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg
    13441 cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg
    13501 acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat
    13561 ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc
    13621 gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg
    13681 agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg
    13741 acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag
    13801 acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa atcactcgtg
    13861 cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa agtggttaac gtcgatttcg
    13921 tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc
    13981 tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg
    14041 aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca
    14101 aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg
    14161 aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg
    14221 acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag
    14281 cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg
    14341 agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg
    14401 atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttatg
    14461 ttggtgataa gaacgtgaaa gcggttgaag gcggcaaact ttctgacatc gcgccgacca
    14521 tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg
    14581 tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac
    14641 cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt
    14701 tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata
    14761 tcgccgcaaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac
    14821 aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa
    14881 acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg
    14941 agcagcaaaa agccgcccag gagcgcagcc tcgccgcgca actggatgcc gcattccgtc
    15001 agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc
    15061 gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga
    15121 agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa caaagcgagc
    15181 aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg
    15241 agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa
    15301 gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg
    15361 cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag
    15421 aagcgacgcg caaaggcacc acctataaac cgaccgaaag cgaaaaatcg ctgatgtccc
    15481 gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc
    15541 tgcatcgcta tggtgaacag ctacagggcg aactacgctg gaaaggaatg gttatcggtg
    15601 cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg gctgactggc
    15661 tgcaaggtta cggtctggtg gtggtggttg agcacggtaa aggcgacatg agtctttacg
    15721 gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg
    15781 cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa attcgccgcc
    15841 agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc
    15901 gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt gctggcaaac
    15961 ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga
    16021 tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga
    16081 ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggca ccactgagta
    16141 aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca
    16201 ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac cacatgggta
    16261 gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcaggcactg gagcgttaca
    16321 atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag
    16381 gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag aacgaagcgg
    16441 acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg
    16501 ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt tataacctgc
    16561 cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatactt
    16621 ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc
    16681 tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa
    16741 gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg
    16801 gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat
    16861 gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc
    16921 ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca taccggaagt
    16981 aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc gcaccgcatg
    17041 gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa tcgtaatttt
    17101 gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat agtgacgttg
    17161 ataattaagg tttttattcc cttgtctatg caaccgactc actgacgtat tatgcagata
    17221 atatttatat aatgattgct cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt
    17281 tgtccagaca atatcctgat gatgtaatcc ggcaataaat ttaatgttat ttttaacaat
    17341 aacatcacgg cgataaaccc ccatccagac aacgtgagtc caacggcgcg aagaaagccc
    17401 catccgcagc cagtccgggc cggttaatac gccggttgag cgaaggcgat cgctggggat
    17461 ggattgccag gtttctcccg tttcacgaaa acaccagtca gcgttgcact gcgccacgtc
    17521 gaggtcatcc tctaacgcca tggtcatcag cgtttcgtac atggtgggat agacctcatc
    17581 gtcagcatcg acaaaagcga catatttgcc cgtcgccacc tcgatcccac gattacgcgc
    17641 taccgatgcg ccagcattcg cctgatgcaa caaacgaacg tgcggatagt tttctgcgta
    17701 atgctttgct atttcaacag aattatccgt tgaaccatcg ttaataataa tgatttccag
    17761 agcagtccag gtttgcgtaa ttaaagattc catacaagtg cggaaatcat cgcccgcatt
    17821 atataacgga ataataacac taagtttatt ggtgctgttc atcataaatt accgacgacc
    17881 tctgaagtta tcctgacgtt ttacaggctt tgta
//
LOCUS       DAFAVF010000082        17824 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFAVF010000082 DAFAVF010000000
VERSION     DAFAVF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.082"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1383
                     /gene="tnaA"
                     /locus_tag="LGK42_002778"
     CDS             <1..1383
                     /gene="tnaA"
                     /locus_tag="LGK42_002778"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBK2659007.1"
                     /translation="RIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLTDSG
                     TGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVL
                     IKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNF
                     DLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSA
                     RFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSF
                     FDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGL
                     EEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGR
                     DPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVL
                     RHFTAKLKEV"
     gene            1474..2721
                     /gene="tnaB"
                     /locus_tag="LGK42_002779"
     CDS             1474..2721
                     /gene="tnaB"
                     /locus_tag="LGK42_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBK2659008.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2854..4029
                     /gene="mdtL"
                     /locus_tag="LGK42_002780"
     CDS             2854..4029
                     /gene="mdtL"
                     /locus_tag="LGK42_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBK2659009.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4004..4963
                     /gene="yidZ"
                     /locus_tag="LGK42_002781"
     CDS             4004..4963
                     /gene="yidZ"
                     /locus_tag="LGK42_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBK2659010.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5120..5869
                     /locus_tag="LGK42_002782"
     CDS             5120..5869
                     /locus_tag="LGK42_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBK2659011.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5891..6457
                     /gene="yieF"
                     /locus_tag="LGK42_002783"
                     /gene_synonym="chrR"
     CDS             5891..6457
                     /gene="yieF"
                     /locus_tag="LGK42_002783"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBK2659012.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6511..7848)
                     /gene="adeP"
                     /locus_tag="LGK42_002784"
     CDS             complement(6511..7848)
                     /gene="adeP"
                     /locus_tag="LGK42_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBK2659013.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8015..8680
                     /gene="yieH"
                     /locus_tag="LGK42_002785"
     CDS             8015..8680
                     /gene="yieH"
                     /locus_tag="LGK42_002785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBK2659014.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8747..9220
                     /locus_tag="LGK42_002786"
     CDS             8747..9220
                     /locus_tag="LGK42_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBK2659015.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9269..9856
                     /locus_tag="LGK42_002787"
     CDS             9269..9856
                     /locus_tag="LGK42_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBK2659016.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(9918..10640)
                     /locus_tag="LGK42_002788"
     CDS             complement(9918..10640)
                     /locus_tag="LGK42_002788"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK2659017.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10655..11824)
                     /locus_tag="LGK42_002789"
     CDS             complement(10655..11824)
                     /locus_tag="LGK42_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK2659018.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDECVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11851..13467)
                     /gene="bglH"
                     /locus_tag="LGK42_002790"
     CDS             complement(11851..13467)
                     /gene="bglH"
                     /locus_tag="LGK42_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBK2659019.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13553..14947)
                     /gene="bglB"
                     /locus_tag="LGK42_002791"
     CDS             complement(13553..14947)
                     /gene="bglB"
                     /locus_tag="LGK42_002791"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBK2659020.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14966..16843)
                     /gene="bglF"
                     /locus_tag="LGK42_002792"
     CDS             complement(14966..16843)
                     /gene="bglF"
                     /locus_tag="LGK42_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBK2659021.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAAKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(16976..17812)
                     /gene="bglG"
                     /locus_tag="LGK42_002793"
     CDS             complement(16976..17812)
                     /gene="bglG"
                     /locus_tag="LGK42_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBK2659022.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4483 a         4465 c         4240 g         4636 t
ORIGIN      
        1 cgcattcgtg ttattgagcc agtaaaacgt actactcgcg cttatcgtga agaagcaatt
       61 attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat cgatttactg
      121 accgacagcg gcaccggggc ggtaacccaa agtatgcagg cagcgatgat gcgcggcgac
      181 gaagcctaca gcggcagccg cagctactat gcgttagccg agtcagtgaa aaatatcttt
      241 ggttatcaat atactattcc gactcaccag ggccgtggcg cagagcaaat ctatattccg
      301 gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat ggtggcattc
      361 tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg taccgtgcgt
      421 aacgtctata tcaaagaagc cttcgatacg ggcgtgcgtt acgactttaa aggcaacttt
      481 gacctcgaag gattagaacg cggtattgaa gaagttggcc cgaataacgt gccgtatatc
      541 gttgcaacca tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc aaacttaaaa
      601 gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg taatggactc cgcacgcttt
      661 gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg gaccatcgag
      721 cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc caagaaagat
      781 gcgatggtac cgatgggcgg cttgctgtgc atgaaagacg acagcttctt tgatgtgtac
      841 accgagtgca gaaccctttg cgtggtgcag gaagggttcc cgacatatgg cggcctggaa
      901 ggcggcgcga tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct cgactggctg
      961 gcttatcgta tcgcgcaggt acagtatctg gtcgatggtc tggaagagat tggcgttgtc
     1021 tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct gccgcatatc
     1081 ccggcagacc agttcccggc acaggcgctg gcgtgcgagc tgtataaagt cgccggtatc
     1141 cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg
     1201 ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac tcaaacacat
     1261 atggacttca ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa tattaaagga
     1321 ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact gaaagaagtt
     1381 taattaatac tacagagtgg ctataaggat gttagccact ctcttaccct acatcctcaa
     1441 taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga aaaaaagcac
     1501 tctgcatttt ggggtgttat ggttatagca ggtacagtaa ttggtggagg tatgtttgct
     1561 ttacctgttg atcttgccgg tgcctggttt ttctggggtg cctttatcct tatcattgcc
     1621 tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa ttatcctgtc
     1681 ggctccagtt ttaacaccat caccaaagat ttaatcggta acacctggaa cattatcagc
     1741 ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc tgctaatggt
     1801 gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc acgtattgtc
     1861 gggatctgca cagccatttt cgttgccagc gtattgtgga taagttcgtt agccgccagt
     1921 cgcattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt gtttggttct
     1981 ttcttcttcc aggtcgatta ctccatcctg cgcgatgcca ccagcaccac tgcgggaacg
     2041 tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt tggtttccac
     2101 ggcaatattc ccagcctgat tatttgctat ggaaaacgca aagataagtt aatcaaaagc
     2161 gtggtatttg gttcgctgct ggcgctggtg atttatctct tctggctcta ttgcacgatg
     2221 gggaatattc cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa cgttgattcg
     2281 ctggtgaaat cgttcctcgg caccaaacag catgggatta tcgagttttg cctgctggtg
     2341 ttctccaact tagctgttgc cagttcgttc tttggtgtca cgctgggatt gttcgattat
     2401 ctggcggatc tgtttaagat tgataactcc cacggcgggc gtttcaaaac cgtgctgtta
     2461 accttcctgc cacccgcgtt gttgtatctg atcttcccga acggctttat ttacgggatc
     2521 ggcggtgccg gactgtgcgc caccatctgg gcggtcatta ttcccgcagt gctggcaatc
     2581 aaagctcgca agaagtttcc caatcagatg ttcacggtct ggggcggtaa tcttattccg
     2641 gcgattgtca ttctctttgg tataaccgta attttatgct ggttcggtaa cgtctttaac
     2701 gtgttaccta aatttggcta aatccttcag gaagccagcc atttcgctgg cttcttgcct
     2761 gtcaggaaat cacttatgtc caaatggcaa ctcgcctgat tctccttcac cacgtatgct
     2821 ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt
     2881 gccctggttt tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc
     2941 gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctctgt ttatctggct
     3001 gggatggcag ctgcgatgtt atttgccggt aaagtggccg atcgttcagg gagaaagccg
     3061 gtcgccatcc ccggcgcggc gctatttatt atcgcctcgg tgttctgttc actggctgaa
     3121 accagcacgt tatttcttgc agggcgcttt ctacaggggt tgggcgcggg ctgttgttac
     3181 gtggtggcgt tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc aaaagtgctg
     3241 tcattactca acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg
     3301 attatgctta aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg
     3361 gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc ctgcggcccc cgcagcttcg
     3421 gacaaaccac gagaaaatag cgagtcgctg cttaaccgtt ttttcctcag ccgtgttgtt
     3481 atcaccaccc tcagcgtttc ggtgatcctc actttcgtca atacatcgcc ggtattgctg
     3541 atggaaatca tgggttttga gcgcggagaa tacgccacca ttatggcgtt gactgctggc
     3601 gtcagcatga ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg
     3661 ttgatgatca cctcgcaggt gttattcctt gcagcgggga tcacccttgc cgtttcacct
     3721 tcccatgcgg tttctctgtt tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt
     3781 ggcgtagcga tgagtcaggc gttaggacca ttttcattac gcgcgggcgt agccagctcg
     3841 accttaggta ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt
     3901 ggtatcggcg catggaatat gctgatcggg attctgattg cctgtagcat agtgagcctg
     3961 ttgctgatta tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac
     4021 cacgcttgat ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac
     4081 caaagcggcg aagcggatga acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt
     4141 aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctgggcctgt cgcccacacc
     4201 gctaatggtc agcatggagc aaaatctggc ggaatggatg caaatgagca atctgctgct
     4261 ggataaaccg caccaccaga caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc
     4321 gctgatgatg atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc
     4381 gaccatcaaa ctacgtaact gggattacga ttccttagat gccattactc gtggtgaagt
     4441 ggatattggt ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct
     4501 accgttagcc attgattatg aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa
     4561 agatcatccg gcactgcatg aaacgtggaa tctggacacc tttttacgtt atccgcatat
     4621 cagcatttgc tgggaacaga gcgacacctg ggcgctggac aatgtgttac aggagctggg
     4681 gcgcgaacgc acgattgcta tgagcctgcc ggaatttgag cagtcactgt ttatggcagc
     4741 gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact
     4801 ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct
     4861 ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg
     4921 gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg
     4981 cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt
     5041 tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc
     5101 attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca
     5161 tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccttca
     5221 tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta
     5281 tggcattggc gaattgccgg aaattgtcac cctgccgaaa ggtaaaccgg ttttcagcga
     5341 taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac
     5401 aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag
     5461 cccacacgcg cccgataacc acaccttttc aagcaatgaa tcgctatgga tcagtaaaca
     5521 aaacgatcct aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact
     5581 aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg
     5641 cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggaag acgtgctggt
     5701 gtggtccgtg gcggtcacgc ccacgattga aaagctcagt gtctgggagt tagatggcaa
     5761 acacggctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat
     5821 gatgcgtttt gcccagctct ctaccgtgaa ggctttttcg ccaaattgat agacaaccac
     5881 aggagtcatc atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg
     5941 ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcaa gcatggaagt
     6001 caatgcgtta ccttccattg ccgacattcc tctgtatgac gctgacgtac agcaggaaga
     6061 cggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt
     6121 gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg
     6181 gctttcccgc ctgccggatc aaccgctggc aggtaaaccg gtgttgattc agaccagctc
     6241 aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct
     6301 cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga
     6361 tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac
     6421 cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat
     6481 gcatggcggg tttttaatgc gctatcgatt ttagtgagcg tcgataaata caatcttcag
     6541 gataaacatc agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc
     6601 aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct
     6661 gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt
     6721 cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc cagctgcagc
     6781 gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga acagcagacc
     6841 aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat
     6901 ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac
     6961 atacagcgcc tgcttcatgc gcgggaattt acccttctca tccgccaggc ctgctttatc
     7021 ggtcacgcca atcagcgtac cggaggagtc aaacaggttg accagcatga aagagaaaat
     7081 cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatggccca cgacagtcat
     7141 tacgctcggc ggcgcagaaa cgatgccatt gtagtggaca tcacccagca tccagcccag
     7201 cagcgttgtc accacgatgg aaaccagcac cgccgcgtga atgttgcgcg aggccagaat
     7261 agcaatgatg aagaagccga ggatccccag cagcacgctg tgagaagtca ggttaccgat
     7321 gctgaccagc gtttccgggt tagcgacaat cacacctgca ttcttcagtc ccatcatacc
     7381 aatgaacaga ccgataccgc tggtgatgcc cacacgcaga cttaccggaa tgttagcaat
     7441 catccagtaa cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc cccagaagat
     7501 tgcgcccatc ccgacttgcc acggcaagcc catagcctgt acaacgacaa aagcgaagaa
     7561 tgcattcagg cccatagcgg gtgccagtgc aactggcagg ttagcaaaca gtcccatcat
     7621 aatactgccg aatgcagcaa tcagacaggt agtaacgaag acggcgctgg tatccatgcc
     7681 agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt
     7741 aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg
     7801 ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta tctatttccg
     7861 aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg gacggctgaa
     7921 gataactttt tttcattgta tttgcttata cggcaacgat tgcgtcatgc gaattggcat
     7981 gacgaaaacg ttaaactaac gataaaggaa agacatgtcc cagatagaag cggtattttt
     8041 cgactgcgac ggtacgctgg tcgacagtga agtcatttgc tcccgcgcat atgtaacgat
     8101 gtttcaggaa tttggtatta cgctcgatcc tgaagaggta ttcaaacgtt tcaaaggtgt
     8161 aaaactgtac gaaattatcg atattgtttc ccttgaacat ggtgttacgt tagcgaaaac
     8221 agaagctgaa cacgtttacc gtgcagaagt cgctcggctg ttcgattcag aactggaagc
     8281 catcgaaggg gctggagcgc tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa
     8341 cggcccaaat aacaaaatgc agcattctat gggcaagctg aatatgttgc actacttccc
     8401 ggataaactg ttcagcggct acgatattca gcgctggaag ccagacccgg cgttaatgtt
     8461 ccatgcggca aaagcgatga atgtaaatgt agaaaactgc attctggttg atgactcagt
     8521 tgccggtgcg caatctggta tcgacgcagg tatggaagtg ttctacttct gcgccgaccc
     8581 gcacaataag ccgatcgttc acccgaaagt caccaccttt acccatcttt cgcagttacc
     8641 ggaactgtgg aaagcgcgtg gttgggatat tacggcatag ttcttcacac tcccttcact
     8701 taccccgctt aaattggcgc tcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg
     8761 tcgggtaata catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt
     8821 tcttgtcctc tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt
     8881 acttccattg tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg
     8941 cgtgatggtc gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc
     9001 tggtggcata ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa
     9061 gggcatggct tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg
     9121 catcattcct gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg
     9181 attggatatt tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg
     9241 gctactcaaa agcagacagg atgttactat gactcaaaat atcaggccgt taccccaatt
     9301 caaatatcat cccaagccac tggaaacagg cgcatttgaa caggataaaa ccgtagagtg
     9361 cgattgctgt gaacaacaga cgtcagttta ttactcgggt cccttttatt gcgttgatga
     9421 agttgaacat ctctgtccgt ggtgtattgc ggacggttct gctgctgaaa agtttgcagg
     9481 tagttttcag gatgatgcca gcatagaagg tgttgaattt gagtatgatg aagaggacga
     9541 atttgccggt attaagaaca catatcctga tgaaatgctg aaagagttgg ttgaacgcac
     9601 gccaggttat catggatggc aacaggaatt ctggcttgcg cattgtggcg atttctgtgc
     9661 ttttatcggc tatgtgggct ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct
     9721 tgaagaagat tgtgagaatt tcggtattag aaattctgat ctagctaaat gcctgcaaaa
     9781 gcgtggtgat tgtcagggtt atctcttccg ctgtctccac tgcggcaagc tgagactgtg
     9841 gggtgatttt tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatgcatcc
     9901 ggccttattt cttcagctta accgagcgct aattctgcgg cagcggcttt atcagcgatt
     9961 accatcagcg aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct
    10021 tgcagcacat ttttcagtgc ctgcgcttta cctgcgccac tgacaattat cagcaagttt
    10081 ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag
    10141 tcgccgccca actcaccatg cgccacaata tcaaccatct ctccctggat cgggaactcc
    10201 acggtttgct catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca
    10261 cctaatccca gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg
    10321 cggtagttat caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa
    10381 aacagattac gcagattggt aatcgttacg ccttctccct ctttgccacg aaagggaatt
    10441 tcatcgaaat tatagaaata gcagttatcg taccagggct tacctttaac cagggtggtg
    10501 aggtattcat acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc
    10561 gtcttcgaca tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct
    10621 tcggtaatga ttaatttcat cttaatgcct tattttattt aaataatttc tgtacgaagg
    10681 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
    10741 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
    10801 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
    10861 gatcgttcag ccgggccgcg acacattcat ccggtacggt tgtcgtggta acaccactga
    10921 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
    10981 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ttaaaacgct
    11041 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg
    11101 gataaaagac tttacgtctt tcctgaggaa cgaaatcttc gggaataatg cccttcgcat
    11161 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
    11221 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccggta tcaccaaaac
    11281 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
    11341 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
    11401 caccatgcgc aacagaacgc gtatccagat aactgcctgg caccagaatc atactggagt
    11461 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
    11521 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
    11581 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaccaccc accactacag
    11641 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
    11701 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
    11761 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
    11821 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
    11881 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
    11941 tttgatatac gtggcgtaga aacgaatttc cggacgcgag gtcaacatac tggtattgac
    12001 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
    12061 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
    12121 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
    12181 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
    12241 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
    12301 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttact
    12361 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
    12421 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatagc cgttattatc
    12481 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
    12541 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat tggtattaat
    12601 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
    12661 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
    12721 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
    12781 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
    12841 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
    12901 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
    12961 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
    13021 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
    13081 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
    13141 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
    13201 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
    13261 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
    13321 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gatgttccac
    13381 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
    13441 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
    13501 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
    13561 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
    13621 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
    13681 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
    13741 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
    13801 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt gcgcctaatc
    13861 cgttctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
    13921 gtaatccaac cgaatcaatt tgccaccccc actctgaact tttcagatgc ggattgggga
    13981 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
    14041 cagtcatgta ataactaaaa gagatgaaat cgacggtatg ttttaaatct tctgcgtcac
    14101 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
    14161 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
    14221 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
    14281 cgattttcgc ttcggggagc aggctatgac aggctttaac tgcccgcgca ctggcaacca
    14341 gttgatggtg gatagcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
    14401 ccgtgaatgg ctcgtgtaac gacatgttga tttcattaaa cgtcagccat aacgccactt
    14461 tatgttggta gcgagtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgctc
    14521 gattagccca accgccgtag tttttcacca gcccatatgg catttcgtaa tgggataacg
    14581 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    14641 accccgcttc attcggttcg acttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    14701 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    14761 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    14821 ttcccattac gccatgaggc tgtaaatctg aggtcgagat ccctttgcca tcttcctgcc
    14881 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    14941 ctttcataat taacttcttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    15001 ttatctgcgc cgtgccgtgg ggtaatacgt ccgtaaaatc atcgctatta ctgattaata
    15061 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    15121 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    15181 gttttacggt gtcgataccg acatgaatca ggagctccac accatcatct gactcaatgc
    15241 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    15301 caccaaccga gggcagaatg gcgatacctt tacccaacag gccactggca aacgtggtat
    15361 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    15421 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
    15481 tagcggtgat gaaatgaagc atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac
    15541 cgccaataac gctggcccag acggtgaaat caattcccgt tgacgggatg gtttgcatga
    15601 aggtgaaaat acttggcaaa ccaaaggagt acactttcgt ttgcgcgtag ccaatgatgg
    15661 tggcccccaa agccccactg atacaggcga taacaaaggg gtacttacgc ggcagattaa
    15721 cgccatatac cgctggttcg gtgataccaa acaggcccgt caacgccgct gatcccgcca
    15781 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    15841 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    15901 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    15961 cgcccattac cgcgcctgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    16021 caatcagttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    16081 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    16141 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    16201 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccagc tcatccgctt
    16261 tttgcccgtt ctcgaaagca gtcagaatta atggatgcac taacgctcca ccaatcacca
    16321 tggcagtaaa tggattaccg ccgaagcgtt tcgccgcggt gtatcccagg attatcggga
    16381 agaaccaaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    16441 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
    16501 tcaaaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    16561 tacctttatc atcattttcc ggtgcctgtt gcgctttttc gtcaaggcct gccacactgt
    16621 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tggccaccgc
    16681 tttccaccac cataataata ccgggggtct ttttcagtac ctctgcttgc gctttgcttt
    16741 catcctttaa cttaaaacgt aatcgcgttg cgcaatgcat cagactcaca atgttatctg
    16801 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    16861 tttgcggcaa aactcaaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    16921 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
    16981 gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgcg ggtgagattt
    17041 tacgttgata ctgcaaacca ataaaaatgg cgatccgctc cgcacattgc catgcttgcg
    17101 ggtaattttg ttttactgct tgttgtaatg actcatcact atcgttaatt gaagcatgtc
    17161 caagaatacg ccaggataaa aacttcagat gcgtaaccag tcgctgataa ctcaagcttt
    17221 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaatt
    17281 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    17341 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    17401 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcagcg
    17461 ggttgggcag taacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    17521 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataatac
    17581 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
    17641 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    17701 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    17761 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    17821 cctt
//
LOCUS       DAFAVF010000083        17678 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFAVF010000083 DAFAVF010000000
VERSION     DAFAVF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.083"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..84)
                     /locus_tag="LGK42_002794"
     CDS             complement(<1..84)
                     /locus_tag="LGK42_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HBK2659023.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESM"
     gene            complement(130..630)
                     /locus_tag="LGK42_002795"
     CDS             complement(130..630)
                     /locus_tag="LGK42_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBK2659024.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(716..895)
                     /locus_tag="LGK42_002796"
     CDS             complement(716..895)
                     /locus_tag="LGK42_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBK2659025.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1276..2082)
                     /gene="trpA"
                     /locus_tag="LGK42_002797"
     CDS             complement(1276..2082)
                     /gene="trpA"
                     /locus_tag="LGK42_002797"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBK2659026.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(2082..3275)
                     /gene="trpB"
                     /locus_tag="LGK42_002798"
     CDS             complement(2082..3275)
                     /gene="trpB"
                     /locus_tag="LGK42_002798"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBK2659027.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3287..4648)
                     /gene="trpCF"
                     /locus_tag="LGK42_002799"
     CDS             complement(3287..4648)
                     /gene="trpCF"
                     /locus_tag="LGK42_002799"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBK2659028.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPRASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(4649..6244)
                     /gene="trpD"
                     /locus_tag="LGK42_002800"
     CDS             complement(4649..6244)
                     /gene="trpD"
                     /locus_tag="LGK42_002800"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBK2659029.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6244..7806)
                     /gene="trpE"
                     /locus_tag="LGK42_002801"
     CDS             complement(6244..7806)
                     /gene="trpE"
                     /locus_tag="LGK42_002801"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBK2659030.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7828..7921)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7898..7942)
                     /gene="trpL"
                     /locus_tag="LGK42_002802"
     CDS             complement(7898..7942)
                     /gene="trpL"
                     /locus_tag="LGK42_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBK2659031.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8080..8961
                     /gene="yciV"
                     /locus_tag="LGK42_002803"
     CDS             8080..8961
                     /gene="yciV"
                     /locus_tag="LGK42_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBK2659032.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8958..9578
                     /locus_tag="LGK42_002804"
     CDS             8958..9578
                     /locus_tag="LGK42_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK2659033.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            9679..10551
                     /gene="rluB"
                     /locus_tag="LGK42_002805"
     CDS             9679..10551
                     /gene="rluB"
                     /locus_tag="LGK42_002805"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBK2659034.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(10591..11181)
                     /gene="cobO"
                     /locus_tag="LGK42_002806"
     CDS             complement(10591..11181)
                     /gene="cobO"
                     /locus_tag="LGK42_002806"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBK2659035.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11178..11936)
                     /locus_tag="LGK42_002807"
     CDS             complement(11178..11936)
                     /locus_tag="LGK42_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBK2659036.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12156..13205
                     /gene="sohB"
                     /locus_tag="LGK42_002808"
     CDS             12156..13205
                     /gene="sohB"
                     /locus_tag="LGK42_002808"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBK2659037.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13241..13492)
                     /locus_tag="LGK42_002809"
     CDS             complement(13241..13492)
                     /locus_tag="LGK42_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBK2659038.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13872..16469
                     /gene="topA"
                     /locus_tag="LGK42_002810"
     CDS             13872..16469
                     /gene="topA"
                     /locus_tag="LGK42_002810"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK2659039.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16679..17653
                     /gene="cysB"
                     /locus_tag="LGK42_002811"
     CDS             16679..17653
                     /gene="cysB"
                     /locus_tag="LGK42_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBK2659040.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4308 a         4426 c         4628 g         4316 t
ORIGIN      
        1 catggattca agcatagatt cggcttgctt ttccattgcg tgggcgtcac gtaaccagtc
       61 atgataatgt tcaatacgat tcatgaaaat gtcctcttta taaaatgact aaaagttaaa
      121 attcatattt caggctttat tttcggcttt cttatttacg ttattaaggg ccagatcagt
      181 cagtttgatg tcggtggcct tttcttcttc cagggtttct ttcagaagct tcgctgcttt
      241 acggtaacct aattgttcag ccagcgtcgc taatgtcccg taactggcaa tctcataatg
      301 ctcgactttc tgtgctgcgg caatcagtgc ggcatcacgc acttcgtttt tctcggtact
      361 ttcgatgacc tcattagctt cttcaataag accttccatt gccacacatt tcatgcgctt
      421 aattttcagg ttcgattcgg actccacaac ttgatcaata cgttcaatct gtccatgagt
      481 ttcctcgagg tgcgcatgaa aagcctgact taatttttca tttgatgttg ctcttgcgag
      541 ttttgccagt gcccgggtta attgtttttc tgcgctgtag gtatctgaaa gcaggtgaat
      601 aaatacatct tcaatggtct tcatattcat atttttctcc agtgaaatca ctgcgagcca
      661 tacggcccgc agatgcaatt atctttatca gctgatatta ataattaacg gtgaatcagg
      721 atttacctga tttattacca ccgctttgtt gaccgccttt tttacccgct tcagatgcgc
      781 gttgcggatc atttttaaaa ttaccgccgc tatgctgacc gcctttacgg cctgcgtcgg
      841 atgccttctc acggtcttcg gcgaaatttc ctgaaccacc acgatgttcg gccatgttat
      901 ttctcccgtt gcgttgcatt gtttcattaa tatgagtgtt gtgtgtcgac actcattaaa
      961 attagtcgct aatgagaatt agtcaaatta agcgcaacga gaagatagag ggaaaatata
     1021 ttttgaggaa cattctggat atattaacaa ttacctgagg aataagtgac ttagagaaaa
     1081 ttattgattt tactggtgtt atgttgcggg attaatttgt ttgattatat tactgttggg
     1141 cggaaaaatg acgtaagttg acgttcgacc ggggtaagcg aaacggtaaa aagataaata
     1201 ttaaatgaat ttaggatttt tccggcttca ttaaagaaag ttaaaatgcc gccagcggaa
     1261 ctggcggctg tgggattaac tgcgcgtcgc cgctttcatc ggttgtacaa aaactttcag
     1321 tgccgccagc attttctctg gctcattaat atgttgctcg atgattttaa caatggccga
     1381 accagaaatc gcgcccgcag ctcctgcatc aatcgctgct tttacctgat ccggggcgga
     1441 aataccaaac ccctgcaatg gaggtgcagc gttgtactct ttcagcttcg caaccagatg
     1501 attgaggggt aacgcggcgc ggttttctgc gccggtcacg cccgctcgtg acagcaaata
     1561 ggtgtaacca cgaccgtaag aggctatctg gcgcagcagg tcgtcatcgg catttggcgg
     1621 gcagatgaag ataggtgcga cattatggcg caacgcggcc tggcggaagg gcgcggactc
     1681 ttcaattggc acatcggcaa ccagcaccga atcgacgccg actttctcgc actgggcata
     1741 aaactcatca atgcctttgt taaacaccag attggcatac atcaacaggc caatgggaat
     1801 ggtcgggtgt ttctggcgaa tcagtgccag catttcaaaa cattgtgccg gagtcacacc
     1861 tgccgcaaag gcgcgcagag tggcgttttg aatcgtcggg ccatccgcca gtgggtcgga
     1921 gaaggggata cctaactcca gcgcgtcagc accggcttca attagcgtat cgataatttt
     1981 caatgactgc tcaatgcccg gatcaccgag cgtgacgaaa ggaacgaatg cgccttcttt
     2041 gcgctccttc aactgggcaa acagagattc gtagcgttcc atcagatttc ccctcgtgct
     2101 ttcaaaatat cgtgaacggt gaagatgtct ttatcgccgc gaccggaaag gttaaccacc
     2161 agtagctgct ctttatccgg gttttcgcgc atcattttca acgcatgggc cagggcgtgg
     2221 gaggattcca gcgccgggat gatcccttcg tgcaggcaca gcgttttgaa ggcttcaagg
     2281 gcttcatcat cggtaataga cacgtaatca gcgcgtccag tgctgttaag atacgcgtgt
     2341 tgtgggccga cagacgggaa atccagtccg gcggagatgg agtaagattc ttcaatctgc
     2401 ccgtcttcgg tttgcatcat cggcgctttc ataccgaaat agatgcccac gcgaccatgt
     2461 tttaacggtg cgccgtgctc gccagtttcg ataccgtgac cacctggctc cacaccaatc
     2521 aggccgacgt tggtttcatt gatgaaatca gcaaacatgc cgatggcatt cgaaccgccg
     2581 ccaacacagg cgataacggc atccggcagg cgaccttctc tttccagaat ctgcgctttg
     2641 gtttcttcgc caatcatccg ctgaaactca cgcacaatgg tcggataagg atgcgggcca
     2701 gctgcggtgc ccagcatata gtgcgcggtt tcgtaactac cggaccagtc gcgcagcgcc
     2761 tcgttacagg catctttcag cgtcgcggaa ccgctatgca ccgggatcac ttccgcaccc
     2821 attaagcgca tacgaaaaac gttaggcgac tggcgttcta cgtctttggc acccatataa
     2881 atacggcatt tcaggccgag cagggcgctg gcaagggccg acgccacgcc atgctgaccg
     2941 gcaccggttt cggcgatgat ttcggtttta cccatccgct tcgccagtaa agcctgaccg
     3001 agcacctggt tagttttatg cgcgccgccg tgcagcaaat cttcacgctt gagatacaga
     3061 gtggtgttcg tcccggctgt aatgttctgg catttggtca gcgcggttgg acgcccggca
     3121 tagtttttca gcaggtcgtt gaactgagcc tgaaattcag gatctttttg cgcactgaca
     3181 aaagcttctt ccagctggcg cagagcaggc atcaggattt gtggcacgta catgccgcca
     3241 aactcaccaa aataggggtt aagtaatgtt gtcattgttc ctttccttaa tatgcgcgca
     3301 gcgtctggaa aaccgaggcc aaaagacgtg cgtctttgat gcccggttgc gactctacag
     3361 cagaattaaa atcaagtccg gcgcagccgg tttgtgccgc ttccacgcag ttatctgcgc
     3421 ctaagccccc cgccagcaga acgttgccaa gcgattgacc atttaatagt gaccagtcga
     3481 aacgttgccc gcttccaccc tggccgttgt ctaaaacata tttatcaacg tgctgaagct
     3541 cgcgggcggg cagggtttca ccgacgctta atgctttcca gatggcaacg tgtgctggca
     3601 gagcttcacg cagcgtatcg atatacagct gatcttcatt accatgcagt tgcactgccg
     3661 ccagcgataa caccttagct ttgtccacca catcggcaat atcgtgattg cggaacacgc
     3721 caacatactg caacggtgcc gcagccatca cttcctgcgc ctgttcaacg ttgacgcaac
     3781 gcggtgatgt cgcaacaaaa atcaacccac cgtaaatcgc gcccgcgtca taagctgctt
     3841 tagcatcttg cccacgcgtc aggccacata ctttattctc acccagcaac acccggcgca
     3901 cggcggcgtg caaatcgtca tgggccatca acgccgaacc aatcagaaaa ccgttagcga
     3961 agtggcttaa ctcgcgcacc tgagcgtaag tattgatgcc ggattcgctg attaccgtta
     4021 cgttgtgccc cagtttcggc gcaagctcgc gggtacggtt gagatcaatc gacaaatccc
     4081 gcagatcgcg gttgttgatg ccaacgacct ttgcccccaa tgcaatggca cgctccagtt
     4141 cctcttcatt actgacttcg gtcagcacac ccatctccag actgtgggcg acggcggcaa
     4201 gctggcgata ttgttcgtca tccagtactg aaagcattaa taagcaggca tcagcctggt
     4261 aatagcgcgc cagatagatc tggtaaggat cgataatgaa gtctttacat aaaatcggct
     4321 gcggggcgat ttggctgacg atggggagga aatcaaagct cccctgaaaa tatttctcat
     4381 cagtcagcac cgaaattgcc gaagcgtaat gtttataaat ggcggcaatg cgtgctggat
     4441 cgaaatcatc acggatcacg ccttttgacg gcgacgcttt cttgcactcc agaataaacg
     4501 ccgtgcgcgc accctgtagc gcatcataaa aatgtcgcgt gctcggctga acctcattct
     4561 gaaaactggc ccgcggttgc tgctgtttgc gggtttctac ccaaatcgcc ttgtctgcga
     4621 cgattttcgc taaaacggtt tgcatcattt accctcgtgc cgccagcgcg gtaactctgt
     4681 cgtaagcgga accactgcgc agtacctcaa gaacggtttg cgcattggct tgcagatctt
     4741 catggccatg caggcgcatt aacatggcga cgttcgcagc gacggctgct tcatgggcgg
     4801 cgtcgccttt accttgtaac aaacgtgtta aaatgtcacg gttttcttcc ggtgttccgc
     4861 ctgccagttg ctcctggtgg tagggtgtca ggccaaaatc ttcagcggtc aattgatagc
     4921 tcttaatttc gccgtcatgc agctcggcaa cgattgtcgg cgcgtgtaat gaaacttcat
     4981 ccatcccgcc gctgtgcacc accgccgcgc gttgataccc cagcacgcgc aaggtttcgg
     5041 caatcggcag caccagttcc ggactataaa caccaattaa cgccagcggc ggatgcgccg
     5101 ggttaatcaa tggccccagc acattgaaca gggtgcgggt ttttagttgc tggcgaaccg
     5161 gcattgcatg gcgaaatccg gtgtgatatt tcggtgcaaa gaggaaacat acgcctaact
     5221 catccagcgc ctggcgcgat ttatcggcgt tcatatcaag attaataccg aacgccgcca
     5281 gcagatcgga cgaaccagat ttactggaga cgctacggtt gccgtgtttc gccactttca
     5341 gcccacaggc cgcggcgaca aacgcactgg cggtagaaat attgatactg ttgctgccgt
     5401 caccgccagt accgacgata tcagcaaaca gataatccgg gcgcgggaac ggcgctgcgt
     5461 tttccagtag cgcggttgct gccccggcga tctcgttcgg gtgctcaccg cgaattttca
     5521 tgctcaccag cgccgccgcc agttgttccg gcttcagctc gccacgcacc accgctgaaa
     5581 acagctggtg gctttcttgt tggctaagcg tctgcgcctg atacagtttt tccagaatcg
     5641 gttgcagcgt gttggctggc tctagtttct gctgcgccca ggccagcgtt tgttccagca
     5701 ggcgagcgcc ctgggtggtg agaatggatt ccggatggaa ctggaatcca caaacgcgat
     5761 ccgcatcgtg acgtactgcc atcaccatgc cattaaaatg ggcgttgatg gttaaaccgg
     5821 ccggaatgtt actgccaacc agcgagtgat aacgcgccac cggcagcggg tttgttaatc
     5881 cggcaaacat cgcctgaccg tcatgttcaa tgctcgacgc tttaccgtga agaatttcgc
     5941 ccgcctgacc gacatagccc ccgtaagctt cgacaattgc ctgatgtccg aggcaaatgc
     6001 caataatggg cagcttgcca cgcaagcggg tgaggagttc cggcatacaa ccggcttcgc
     6061 tcggcacacc ggggccagga gaaagcatca gtaccggatt gctcatggtc gccaggcgtt
     6121 caattaaggt ctgcgccgga atatggttgc ggtaaatcac cacgttatgc ccattgctgc
     6181 gcaactgatc tgccaggttg tacgtaaaag agtcgatatt atcgagcagc agtatgtcag
     6241 ccatcagaaa gtctcctgtg catgatgcgc ggtggcaata gcgcgcagta cagcgcgggc
     6301 tttattacgg gtttcgtcgg cttccgactg cggaatagaa tcaaggacta cgccagcacc
     6361 ggcttgcacg gtggcgatac cgttttccac cagcgccgag cggatcacaa tgcaggtgtc
     6421 gagatcgccg tgcgcggtga aataacctac cgcgccgccg tagctgccgc ggcgacgacc
     6481 ttccgcctcg gcaattaact gcatggcgcg cactttcggc gcaccgctta acgtccccat
     6541 attcatacag gcgcggtaag cgtgtagggc gtcgagatcg tggcgcagct caccgaccac
     6601 gcgggagacc aggtgcatca cgtaagagta acggtcaact ttggtgaggt cggcgacgta
     6661 gcggctgccg ggggtgcaaa tgcgtgccag atcattacgg gcaagatcca ccagcatcag
     6721 atgttcagaa agctctttat gatcggtacg catttccagt tcgatgcggc tgtcgaggtc
     6781 tctgtccagc gagccatcgg cacgacgacc gcgtggacgt gttccggcaa tcgggtagat
     6841 ctcaatctgg cggctggtgg cgtcatactt gagcgaactt tccggcgacg cgccaaacag
     6901 ggtgaaatca ttatcctgca taaaaaacat gtacgggctg ggattactct ttttcagcac
     6961 gtaataggcc gccagcggtg acgggcaggg cagagagaaa cggcgagatg gcaccacctg
     7021 gaaaatttct ccagcgcgaa tcgctttttg caacaaacgc actacgccac cgaactcttc
     7081 atcgctctga ttacattcac aacgcatatg cggcacggaa accaccggca gcggcggcgc
     7141 ggcttcggtc agttgctgac gtagttcgtt caggcgagca gtgagacgtt gtttttcttc
     7201 ttcattcgga gcaaacaggc tggcctgaat acgggtgctt tttttctgat ggtcaatcac
     7261 catcagagtt tcagcgagat aaaaacagaa atcagggcag ttattttccg ctgacagttg
     7321 cggtaaatct tcaaatcccg ccacaaggtc ataagagaac aggccgccga agaacattgc
     7381 ttctcgttct tccttcggta cattcaacag attctgcaat aaacggaaag cgtcaaaaac
     7441 cgaaagggag cataagcgag cgtcttcatc cagcagtgga ctgacagggg ggaagcgcag
     7501 cacacggcag tttggtaatt gttcattttc cacacccgca ggcagggcgt tatccagtag
     7561 tgtcagcagg gcttcgccgt tgccggaaag tgcctggatt gtgacagtgt cacctaaagc
     7621 tgtaatgcgc agcgcactgt ctaccagtag caggcttttt aaatcatctt tgctgtcgat
     7681 atctgcggat tccagcagca gcgttgccgg acgatcccca cacaactggt gaaaaagcgc
     7741 ggtgggattg tcgcgataag cgccttcgca ggttagcagt tcgagagtcg gtttttgtgt
     7801 ttgcattgtt attctctaat tttgttcaaa aaaaagcccg ctcattaggc gggctgggta
     7861 tctgattgct ttacgcatgg tgaatacact gcccgtttca ggaagtgcgc caccaacctt
     7921 tcagtacgaa aattgctttc attgtcgata ccctttttac gtgaacttgc gtactagtta
     7981 actagttcga tgattaattg tcaacagctc atttcagaat atttgccaga accgttatga
     8041 tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct tgagcgacac gaattacgca
     8101 gtgatttacg acctgcacag ccataccaca gcttccgatg gctgcctgac gccagaagca
     8161 ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg cgatcaccga ccatgacacc
     8221 acagctgcaa tcgcgccagc aagagaggaa atttcacgtt cgggactggc gctgaatctt
     8281 attcccggcg tggaaatttc cacggtctgg gaaaatcatg aaattcatat tgtcgggctg
     8341 aatattgata ttactcatcc gctaatgtgt gagttcctcg cgcagcagac agaacggcgc
     8401 aatcagcggg cgcagctgat tgccgaaaga cttgaaaaag cgcaaatacc tggggcgctg
     8461 gaaggcgcac aacgactggc gcagggcggc gcagtgacgc gcggtcattt tgcgcgtttc
     8521 cttgttgagt gcggcaaagc cagttcaatg gcggatgtct ttaaaaagta tctggcgcgc
     8581 gggaaaaccg gatacgttcc gccacagtgg tgtacaatag aacaagctat tgatgtcatt
     8641 catcattctg gcggtaaggc ggtgctagct catcctgggc ggtacaatct tagtgctaaa
     8701 tggctgaaaa gattggttgc gcattttgcc gaacaccacg gtgacgcgat ggaagtcgcg
     8761 cagtgccagc aatcgcccaa tgaacgtacc cagctggcgg cccttgcgcg tcagcatcat
     8821 ttatgggcat cacaaggatc tgattttcat cagccatgcc cgtggatcga actgggtcgt
     8881 aaactctggc tgcccgcagg cgttgaaggc gtctggcagc tatgggaaca gccgcagaac
     8941 accacagaga gggaattatg agccagtttt tttatattca tcctgataac ccacagcaac
     9001 gtctgatcaa ccaggcggtg gagatcgtgc gtaaaggcgg ggtgattgtt tatccaactg
     9061 attccggcta tgcgctcggc tgtaaaattg aagataaaaa cgcgatggag cgtatttgtc
     9121 gtattcgcca gctgccggac ggtcacaact ttaccctgat gtgtcgcgat ctttctgaac
     9181 tctcgaccta ttcatttgtt gataacgttg cgtttcgttt aatgaaaaac aacacgccgg
     9241 gcaactatac cttcatcttg aaggggacga aggaagtgcc acgccgtctg ttgcaggaaa
     9301 aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat tgcgcaggcg ttgcttgaag
     9361 cactgggcga accgatgctt tccacttcgc taatgctgcc aggcagcgaa tttaccgaat
     9421 ccgatcccga agaaattaaa gaccgtctgg aaaaacaggt ggatttgatt atccacggcg
     9481 gctatctcgg acagaaaccg acaacggtta ttgatcttac cgatgatacg ccggtcgtgg
     9541 tgcgtgaagg cgtaggtgat gtgaagcctt tcttataagt gcgtaactct gtatactacg
     9601 cggccttaaa atggcgctat acggcgccat tgattctcct gttcgacgcc tgtgaaggcg
     9661 acaccaaaag gaagctctat gagcgaaaaa ttacagaaag tgctggcccg cgctggccac
     9721 ggctcccgcc gtgaaatcga atctattatt gaagccggtc gtgtgagtgt tgatggcaaa
     9781 attgctaaac tcggcgatcg cgttgaagtt acccctgggc tgaaaattcg tatcgatggt
     9841 catctgattt cggtacgtga gtccgctgaa caaatttgtc gcgttctggc ctattacaaa
     9901 ccggaaggtg agttgtgtac ccgtaacgat ccggaagggc gtccgacggt gtttgaccgt
     9961 ctgccaaagc tgcgtggcgc acgctggatt gccgtaggtc gtctggacgt taacacctgt
    10021 ggtctgctgc tgttcaccac cgatggtgaa ctggcaaacc gtttaatgca tccaagccgt
    10081 gaagttgaac gtgaatacgc cgtgcgtgta tttggtcagg ttgacgacgc gaaactgcgt
    10141 gatttgagtc gtggcgtgca actggaagat ggtccggcag cttttaaaac catcaagttc
    10201 agcggcggcg aagggatcaa ccagtggtac aacgtgactc tgaccgaagg ccgtaaccgt
    10261 gaagttcgtc gtctgtggga agcggttggt gtgcaggtga gccgcctgat ccgtgttcgt
    10321 tacggtgata tcccactgcc aaaaggtctg ccacgcggtg gctggacgga gctggacctc
    10381 gcccagacta actatctgcg cgaactggtg gagcttccgc cagaaaccag ctctaaagtc
    10441 gctgtagaaa aagaccgtcg tcgcatgaag gctaatcaga ttcgtcgtgc ggtgaaacgt
    10501 cacagtcagg tgagcggcgg tcgtcgttct ggcgggcgta ataacggtta atcaaagtat
    10561 gccggacgtc atatccggca tttttacaga ttaataatca atccctatct gcgcttttac
    10621 accggcatca aacgcatgtt tgacggggcg taattcactt accgtatctg ccagttcaag
    10681 aatatcccga tgacaaccac gacccgtgat aatcaccgtc tgttgatgtg gacgttcatt
    10741 taacgcctgc accacttctt ccagtggcaa atagtcatac gccaccatat acgtcagttc
    10801 atcaagcaaa accatatcca gtgaggaatc agcaagcatc cgctttgcat gttgccagac
    10861 ttcgcggcag gcggcggtat cagactcgcg gttttgtgta tcccaggtaa agcccgttgc
    10921 catcacctga aactcaacgc catgtggctc cagcagattg cgttcgccat taggccaggt
    10981 gcctttaata aactgcacga cgcctacttt ttttccgtga ccaactgcgc gtgttgccgt
    11041 accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga taataccgcg
    11101 ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct gctgacgctg
    11161 ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg ggcgtcaaag
    11221 gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag cggcatgata
    11281 tcagcgggtg ttttaagttt ctgtggatct tcggtcggga aggcgctggc acgcattgcg
    11341 gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc gctgctgata ttcatcggcc
    11401 agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc ccagttggct
    11461 cgtccctgac gtccaacgct tgatgaagta aagaccagtg aaccggcgtc cgatttgagt
    11521 aataaaggaa gcagtgcctg ggtgagcata aaggtggcat taacgttgac ctgtatgacg
    11581 tcctgccaga cctgtggatt ttgttcgctc attgggcaaa catcgccgag caatccggca
    11641 ttatgcaaaa caccatccag acgcggataa ttaacggtaa tgcgctgtgc cagttgttgg
    11701 caatcttcgg acgtgcaggt cagcaaatcg agaataaacc actgtggctg acgtccagtt
    11761 tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc caacagaatc
    11821 actgtcgcac catagcgtgc atacgtcatc gcggcttcac ggccaatccc atcgctggct
    11881 cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta atgcatggct
    11941 actcctcaac gacgttgtct gtcgtgctgc cagtgtactt tatgactttg gggctttatg
    12001 cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa tggatacttt
    12061 gtcatacttt cgctgcaata acatctctgc gagacggctt aacatgcctg ttgtaaactg
    12121 tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct gaatatggtt
    12181 tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc gccattattg
    12241 tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgggtcaac aatctcagcg
    12301 aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggactca catcagcaaa
    12361 aacagtggca caaagcgcag aagaaaaagc acaagcaaga agcgaaagca gcaaaagcga
    12421 aagccaaact gggcgaggtg gcaactgaca gtaaacctcg cgtctgggtg ctggatttta
    12481 aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg gcggtactcg
    12541 cagcattcaa accgcaggat caggttgtgc tccgtctgga aagccctggt ggcatggtgc
    12601 atggttacgg gttggcggct tcgcagctgc agcgtctgcg tgataaaaac attcctttaa
    12661 ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt gtggcggaca
    12721 aaattgtttc cgcaccgttt gctattgtgg gttccattgg ggtggtggcg caaatgccca
    12781 actttaaccg cttcctgaaa agcaaagata ttgatatcga actgcacact gccgggcagt
    12841 ataagcgtac tctgacgttg ctgggtgaaa ataccgaaga agggcgggag aaattccgcg
    12901 aagagttgaa cgaaacgcat cagttgttta aagattttgt gaagcgtatg cgtccgtctc
    12961 tggatattga acaggtggca acgggtgaac actggtacgg acaacaggcg gtagagaaag
    13021 gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg gaaggccgtg
    13081 aagtggttaa tgtacgctat atgcagcgta aacgactcat tgaccgattc accggcagcg
    13141 cggcagagag cgccgatcga ttgttgctac gctggtggca gcggggtcaa aagccattga
    13201 tgtaaaagac aaacgcgagg ctaagacctc gcgttttgct ttaatcaacc agatgatatt
    13261 tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg cttttggctg
    13321 ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagcacg ccattacgct
    13381 gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga atgattttgt
    13441 tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt ctctttgttc attatttact
    13501 ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat accacgctgg
    13561 tggcaagagc gccttactgg taactttgga ttttgcatgc taataaagtt gcgtatcgga
    13621 ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct tgacattcga
    13681 ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca gtcacctgaa
    13741 ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagaacgagt ccatatcggt
    13801 aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga atcaaattag
    13861 gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa gccaaaacga
    13921 tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac atccgcgatt
    13981 tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccacc aagacggcta
    14041 aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt gacccgtggc
    14101 acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc tctgaactga
    14161 aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac cgcgaagggg
    14221 aagccattgc atggcacctg cgggaagtga ttggtggtga tgatgcgcgc tatagccgag
    14281 tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttaacaaa ccgggtgagc
    14341 tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc gtggttgggt
    14401 atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg tctgtctgcc ggtcgtgtgc
    14461 agtcggtggc ggttcgcctg gtggtcgagc gtgagcgtga aattaaagcg ttcgtgccgg
    14521 aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg ttggcgttgc
    14581 aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa actcaggctg
    14641 cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac aaaccgacaa
    14701 ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc agcacccgtc
    14761 ttggatttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa gcaggctata
    14821 tcacttacat gcgtaccgac tccactaacc tgagtcagga cgcggtaaat atggttcgcg
    14881 gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat cagtacgcca
    14941 gcaaagaaaa ctcacaggaa gcgcatgaag cgattcgtcc ttctgacgtc aatgtgatgg
    15001 cggaatcgct gaaggatatg gaagcagatg cgcagaaact gtaccagtta atctggcgtc
    15061 agttcgtagc ctgccagatg accccagcga aatatgactc cacgacgctg accgttggtg
    15121 cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ttttgatggc tggacaaaag
    15181 tgatgcctgc gttgcgtaaa ggcgatgaag atcgcatctt accagcagtt aataaaggcg
    15241 atgctctgac gctcgttgaa cttacaccag cccagcactt taccaagccg ccagcccgtt
    15301 tcagtgaagc atcgctggtt aaagagctgg aaaaacgtgg tatcggtcgt ccgtctacct
    15361 atgcgtcgat catttcgacc attcaggatc gtggctacgt gcgagtagaa aatcgtcgtt
    15421 tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct tgaagaaaat ttccgcgagt
    15481 taatgaacta cgactttacc gcgcagatgg aaaacagcct cgaccaagtg gcaaatcacg
    15541 aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag cagttagata
    15601 aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt ctgaccagca
    15661 ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc ggggtattcc
    15721 ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc attaacctgg
    15781 tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga tgctgaaacc aacgcgctgc
    15841 gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga cagctatctc atcgatccga
    15901 aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag atcgaagagg
    15961 gcgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa tgtggctctg
    16021 aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac gaagagtgta
    16081 aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa gatccggtgc
    16141 cattacctga gctgccgtgc gaaaaatcag atgcttattt cgtgctgcgt gacggtgctg
    16201 ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg tgaaacgcgt gcgccactgg
    16261 tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat ctggccgatg
    16321 cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt aaaaccaaac
    16381 agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca ttttatgttg
    16441 atggcaaatg ggttgaagga aaaaaataac ctttaattct gtcaggtttt tataaacaaa
    16501 gggtcgcgaa agcggccctt ttttattgca tattattttt tcttcacacc tatacactaa
    16561 ggctataaat gatatagtgg ttatagttag cacctttttt attattaaat cgtattagtc
    16621 acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt ctaagtggat ggtttaacat
    16681 gaaattacaa caacttcgct atattgttga ggtggtcaat cataacctga atgtctcatc
    16741 aacagcggaa ggactttaca catcacaacc cgggatcagt aaacaagtca gaatgctgga
    16801 agacgagcta ggcattcaaa ttttttcccg aagcggcaag cacctgacgc aggtaacgcc
    16861 agcaggacaa gaaataattc gtatcgctcg cgaagtcctg tcgaaagtcg atgccataaa
    16921 atcggttgcc ggtgagcaca cctggccgga taaaggctcg ctgtatatcg ccaccacgca
    16981 tacccaggca cgctacgcgt taccaaacgt tatcaaaggc tttattgagc gttatccccg
    17041 cgtttctttg catatgcacc agggctcgcc gacacaaatt gctgatgccg tctctaaagg
    17101 caatgctgat ttcgctatcg ccacagaagc gctgcatctg tatgaagatt tagtgatgtt
    17161 accgtgctac cactggaatc gggctattgt agtcactccg gatcacccgc tggcaggcaa
    17221 aaaagccatt accattgaag aactggcgca atatccgttg gtgacatata ccttcggctt
    17281 taccggacgt tcagaactgg atactgcctt taaccgcgca gggttaacgc cgcgtatcgt
    17341 cttcacggca acggatgctg acgtcattaa aacttacgtc cggttagggc tgggggtagg
    17401 ggtcattgcc agcatggcgg tggatccggt cgccgatccc gaccttgtgc gtgttgatgc
    17461 tcacgatatc ttcagccaca gtacaaccaa aattggtttt cgccgtagta ctttcttgcg
    17521 cagttatatg tatgatttca ttcagcgttt tgcaccgcat ttaacgcgtg atgtcgttga
    17581 tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc atgtttaaag atataaaact
    17641 gccggaaaaa taatttcttg cgttattttc ggcacctt
//
LOCUS       DAFAVF010000084        17622 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFAVF010000084 DAFAVF010000000
VERSION     DAFAVF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.084"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(43..1635)
                     /gene="lsrK"
                     /locus_tag="LGK42_002812"
     CDS             complement(43..1635)
                     /gene="lsrK"
                     /locus_tag="LGK42_002812"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HBK2659041.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1714..2667)
                     /gene="lsrR"
                     /locus_tag="LGK42_002813"
     CDS             complement(1714..2667)
                     /gene="lsrR"
                     /locus_tag="LGK42_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBK2659042.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2916..4451
                     /gene="lsrA"
                     /locus_tag="LGK42_002814"
     CDS             2916..4451
                     /gene="lsrA"
                     /locus_tag="LGK42_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBK2659043.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4445..5473
                     /gene="lsrC"
                     /locus_tag="LGK42_002815"
     CDS             4445..5473
                     /gene="lsrC"
                     /locus_tag="LGK42_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBK2659044.1"
                     /translation="MLKFIQNNREITALLAVVLLFVFPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKELSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            5473..6465
                     /gene="lsrD"
                     /locus_tag="LGK42_002816"
     CDS             5473..6465
                     /gene="lsrD"
                     /locus_tag="LGK42_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBK2659045.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6477..7499
                     /gene="lsrB"
                     /locus_tag="LGK42_002817"
     CDS             6477..7499
                     /gene="lsrB"
                     /locus_tag="LGK42_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBK2659046.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7526..8401
                     /gene="lsrF"
                     /locus_tag="LGK42_002818"
     CDS             7526..8401
                     /gene="lsrF"
                     /locus_tag="LGK42_002818"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBK2659047.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8425..8715
                     /gene="lsrG"
                     /locus_tag="LGK42_002819"
     CDS             8425..8715
                     /gene="lsrG"
                     /locus_tag="LGK42_002819"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBK2659048.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8772..9530
                     /gene="tam"
                     /locus_tag="LGK42_002820"
     CDS             8772..9530
                     /gene="tam"
                     /locus_tag="LGK42_002820"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBK2659049.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9534..10448)
                     /locus_tag="LGK42_002821"
     CDS             complement(9534..10448)
                     /locus_tag="LGK42_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBK2659050.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10655..12106)
                     /gene="uxaB"
                     /locus_tag="LGK42_002822"
     CDS             complement(10655..12106)
                     /gene="uxaB"
                     /locus_tag="LGK42_002822"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK2659051.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12333..13751)
                     /locus_tag="LGK42_002823"
     CDS             complement(12333..13751)
                     /locus_tag="LGK42_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2659052.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13890..14249)
                     /locus_tag="LGK42_002824"
     CDS             complement(13890..14249)
                     /locus_tag="LGK42_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBK2659053.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14249..15175)
                     /gene="glsB"
                     /locus_tag="LGK42_002825"
     CDS             complement(14249..15175)
                     /gene="glsB"
                     /locus_tag="LGK42_002825"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBK2659054.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15239..16627)
                     /gene="sad"
                     /locus_tag="LGK42_002826"
     CDS             complement(15239..16627)
                     /gene="sad"
                     /locus_tag="LGK42_002826"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBK2659055.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16728..17609
                     /locus_tag="LGK42_002827"
     CDS             16728..17609
                     /locus_tag="LGK42_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659056.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
BASE COUNT         4394 a         4540 c         4412 g         4276 t
ORIGIN      
        1 aaactaacag ccacggtcat catgatgtgg ctgtcaatga aactataacc caggcgcttt
       61 ccataacgac gtcgtcagtc catgatcaac cagccccagc tgatcctgat aaactgcctg
      121 ccacttatcg cgtgaatcct gataaagttc atgcttttcc gggtctggtg tgtgcgtccg
      181 ttcccagcga accaggcgtt ctccggtttc tgccattgat gaaaaaattc cggcaccgac
      241 gccagctgca atggcacatc ctaatgcagt ggcttctttg accaccggaa tattgacggg
      301 taatcccgag acatcagcga gaatttgact ccataatttc ccttttgaac ctccgcctgc
      361 aaagactaac gatgaaggat gaatattcga gaaatcagca atttgctgca agttacacgc
      421 tgatacaatc gccgcatttt cttccagcgc acggaacaat gtcgctttgt tacatttatc
      481 cgggtcaatg gacaagttaa taaaggaagg cgcagcgtga taccaggttt taaagcgcat
      541 tctgtcggag aagatcggca ttacgcccca cgacccaggc ggcacccgac tggccatctc
      601 ttccagcagc gtataggtgt cgatgcctaa acgttcggca atcagttttt cttcggcaca
      661 gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa aagcttatag attcagcttg
      721 taccatgcca ggaataacat gaggattaac gcgcacgttc atttctgggt ctgtcaccgg
      781 cgcggctaaa tttacaactt gctgccagaa tgtgccgcca agaaccgcgg tttgtgccgg
      841 acgcacaacg cctaacccaa ggcaaccaag ctgcacgtcg cctcctccaa cgaccaccgg
      901 agtgcccgcc ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc ccagcaatgt
      961 gccggtttct ttgacaggag aaagaatatc ggcacgtagg ccagccatat ccagcaatgc
     1021 aggtttccag tcacgggtgg ttagatcaag aagtcccgtg gtgccagcgt tagagggatc
     1081 caccgccagt tcgccgctga gcatataggc cagccagtcg ctgatcatgg tgatggttga
     1141 tgcctgacgg taaatatcgg aacgatggtg cgccagccaa agtaatctgg ggatggcact
     1201 taaagccagt gtttgtccgg tcgcgcgata aacttcgttt tcaaaggtat tgttgtgcag
     1261 ttctttaagt tcgctaactt cgcgtgccgc tctggcatcc acattggcgc aggcccagat
     1321 cggggctcct tcattattat ataaaacaat gccttcacgc atcgaacatg ccgaaacggc
     1381 agcgatatac tccggggcta tgccggcgtt gtgcagcgcc tggcgcatac actcacacgc
     1441 cagttgccag tttttgttga gatcaaattc catagaacca ggaacgtccg gtactgccag
     1501 gtgccgccac tccgcctgtc ccactgctat ttgattgcct tccaggtcga atatcacagc
     1561 ccgaatactt ccggtacctg catccagcgc catcaggtaa tactttgatt ctgaaggggt
     1621 aaagagtcga gccattagat tatcctcggc ttatgcgttc tgtctgttcc tctatacgtt
     1681 ctccatcatt cccggtaata aggtcatgca aatttaacta cgtaaaatcg ccgctgctgt
     1741 gtcctgatcg gtaaccagtg cgttgatata accgcctttc attgcagcgg caattgcttc
     1801 ggctttattt tctccccctg ccacgccaac ccggacgggt atggtcttca gcgagcttaa
     1861 aggtaagcca atcagttcgt tatgtatttt aatatccgtg acaacgtcac cttttgcatc
     1921 aaaaaagtag cctaaaatgt cgccaaccgc cccttttcga ccaatcatta actgttcgcc
     1981 ctggctgata taaccggagc gaatgatcgt cgcgtcgtcc tgttgactga cggccccaat
     2041 gccgacaatc gccacatcgg ctgcttgcgc ggctaacaga acatctttga cgcaattttc
     2101 attttttagc gtacgggcaa tgtcagcgga ggatgcccgc aacggagccg gaataatatt
     2161 cacactgcac gccgcgttaa gctgcccgat acccgtcata taagaaccga cgccaccgga
     2221 gagcgtgacc aggcgaattt gctgtgacga aataaaacca cttaagcgtt gcagcgtatt
     2281 catggttgcc tcgccaaaac caatcgccag catctgttgt ggttgaagta aactcatcaa
     2341 catatgcgcc gcgcctatcc ccagtcgccc gccgacatca gcatccgcaa gcccagggat
     2401 cacccggaca tgttgcagcg aaaactgacg acgtaattga gtttcatatt ccagacagcc
     2461 ttcaaagcga gaattaatct gtacgcgaat aatgccggac tgatgccctt tctccagcaa
     2521 gcgcgacact ttcaaacggg tcaggccgag acgatcgctg atctcgctct gggtcagccc
     2581 gtcgtgatag taaaaccacg cgatccgcgc gacctgttct tcttcacaca ttccctgttc
     2641 tgaaattgcc gaatcgttga ttgtcataat tcattcttca ctttgaacat atttaaatct
     2701 ttaatgcaat tgttcagttc ttgcgcattt atatctgtga tggcagccac agtttgactc
     2761 tacgagcatg aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg atctattcgt
     2821 cggaaatatg tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga aatacatttg
     2881 ttcaaaactc acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat acccgcgcgt
     2941 taccgctact ttgcgcccgc tcggtttata aacagtattc aggggtcaat gtcctgaaag
     3001 gcatcgattt tacgttgcat cagggggagg tccacgccct gctcggcggc aatggtgccg
     3061 gtaaatcgac gttaatgaag attattgccg gtattacccc tgctgatagc ggtacgctgg
     3121 agattggggg caacaactac gccagattaa cgccagttca tgctcatcag ctgggcattt
     3181 atctcgttcc ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa aacatcctgt
     3241 ttgggatggc aaaaaaacag ctctccatgc aaaaaatgaa gaacttgctg gcggcgctgg
     3301 gctgccagtt tgatctgcat agtctggcag gatcgctgga tgtcgccgat cgccaaatgg
     3361 tggaaatcct ccgcgggctg atgcgcgact cgcggattct gatcctcgat gaacctaccg
     3421 cctcgcttac ccctgcggaa accgaacgct tgtttagtcg cttgcaagag ctgcttgcta
     3481 ctggcgtggg tattgttttt atctcgcata agctgccgga aattcgccag attgccgatc
     3541 gaattagcgt gatgcgcgac ggaaccatcg ccttaagcgg caaaaccagc gaactgtcta
     3601 ccgacgacat tattcaggcc atcaccccag tggtacggga aaaatcgctc tctgccagcc
     3661 aaaaattatg gctggagtta cctggtaacc gcccacaaca tgctgccgga acgccggtgc
     3721 tgacactgga aaatctgacc ggcgaaggtt tcaggaatgt cagcctgacg ctcaatgccg
     3781 gagaaattct gggcctggct gggctggtgg gagccggacg cacagaactg gccgagacgc
     3841 tctatggtct gcgtactttg cgtggcggac gcattatgct gaatggtaaa gagatcaata
     3901 aattatccac tggagaacgt ttactgcgcg gtctggttta tctgccggaa gatcgccagt
     3961 catccggact gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt actcataacc
     4021 ttcgtggatt ctgggcgaaa accgcgaaag ataatgccac cctggaacgt tatcgtcggg
     4081 cgctgaatat taaatttaac caaccggaac aagctgcacg gacattatcc ggtggcaacc
     4141 agcaaaaaat cctcattgcc aaatgcctgg aagcttcgcc gcaagtattg attgtcgatg
     4201 agccgacgcg cggcgtggat gtctcggcac gtaatgatat ctaccagctg ttgcgcagca
     4261 tcgccgcaca gaatgtggct gtgctgctta tctcctctga tctggaagag atcgaactga
     4321 tggcagatcg tgtgtatgtg atgcatcagg gcgaaattac ccactctgca ctgaccgggc
     4381 gcgatattaa tgtcgagact attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg
     4441 catcatgctg aagtttattc agaacaaccg tgaaatcacg gcattgctgg cggtggtgct
     4501 gctgtttgta ttccccggtt ttctcgaccg ccagtattta agtgtgcaaa cgctgaccat
     4561 ggtttatagc agcgcgcaaa tcctgatcct actggcaatg ggcgcgacgc tggtaatgct
     4621 tacgcgcaat attgatgttt cagtgggttc gattaccgga atgtgcgcgg tgctattggg
     4681 gatgttactg aacgcaggat attcactacc tgttgcctgt gtcgcgactt tactgcttgg
     4741 tttgctcgcg ggatttttca acggtgtcct ggtcgcgtgg ctaaagatcc ctgccattgt
     4801 tgccaccctt ggcacgttag ggttgtacag aggcatcatg ttgctgtgga ctggcggcaa
     4861 atggattgaa gggttacccg ccgaactgaa agagctctcc gcaccgctgc tgtttggcgt
     4921 ttccgcgatt ggttggttga cgataattct ggtggcattt atggcctggc tgctggcaaa
     4981 gacggcgttt ggacgcagtt tttatgccac gggcgataat ttacagggcg ctcgtcaact
     5041 gggtgttcgt acggaagcca ttcgcattgt ggcattttcg ttgaacggct gcatggcggc
     5101 actggcggga attgtgtttg cttcgcagat tggttttatc cccaaccaga ccggtaccgg
     5161 gctggagatg aaagcaattg cagcctgcgt gctgggcggc attagtttgc tcggcggttc
     5221 cggtgcgatc attggtgcgg tactcggcgc atggttcctg acgcagatcg atagcgtact
     5281 ggtgctgttg cgcattccgg catggtggaa cgattttatc gcgggcctcg tgctgctggc
     5341 ggtgctggtg tttgatggac gcctgcgttg tgcgctggaa cgtaatctac ggcggcaaaa
     5401 atatgcccgc tttatgacgc caccgccatc cgttaaaccc gcttcgtcag gtaaaaatcg
     5461 ggaggccgca taatgcgtat tcgctacggt tgggaactgg ctcttgccgc actgctcgtt
     5521 attgagattg tcgcatttgg tgcaattaac ccgcggatgt tagatctcaa tatgttgctg
     5581 ttcagcacca gtgactttat ctgcattggc attgtcgccc taccgctgac gatggtgatt
     5641 gtcagtggcg ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg
     5701 ggcgtactgt ttcaaagtgg tgtgccgatg ccgctggcga tactcctgac cttactgctc
     5761 ggcgcattgt gcgggctgat caacgccgga ttaattatct ataccaaagt taacccgctg
     5821 gtgattacgc ttggcacgct gtatctgttt gccggaagcg ctctgctgct ttccggtatg
     5881 gccggagcga cggggtacga aggtattggt ggattcccga tggcgtttac agatttcgct
     5941 aacctggatg tgctgggact ccccgttccg ctgattatct tcctgatatg tctcctcgtt
     6001 ttctggctct ggctgcataa aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc
     6061 ccgcgcgtgg cgctttatag cgcgattcca gttaaccgca ccttatgtgc gctctatgcc
     6121 atgacggggc tggcgtctgc ggtcgccgct gtgctgctgg tgtcgtattt tggttcagca
     6181 cgttccgatc tcggtgcgtc gtttctgatg cccgccatca ccgccgtggt gcttggcggt
     6241 gccaatattt atggtggttc cggttccatt atcggcaccg ccattgcggt tttattagtg
     6301 ggatatttgc aacaaggttt gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt
     6361 tccggtgcgc tacttatcgt cgttgtcgta ggtcgttccg ttagcctgca tcgccagcaa
     6421 attaaagagt ggctggcgcg tcgggccaat aacccattgc cataaaggat atcttcatga
     6481 cacttcatcg ctttaagaaa atcgccttac ttagcgctct tggcattgcc gcaatctcta
     6541 tgaatgtgca ggccgcagag cgtattgcat ttattcccaa actggttggc gtgggatttt
     6601 ttaccagcgg tggcaacggc gcacaacaag cgggtaaaga gctgagcgtt gatgtgacct
     6661 acgacgggcc gacagaaccc agtgtttctg gtcaggtaca gttgattaat aacttcgtca
     6721 atcaaggtta taacgccatt atcgtttctg cggtttcgcc tgatggcttg tgtccggcac
     6781 tgaaacgcgc catgcaacgt ggtgtgagag tgctgacctg ggactctgat actaaaccgg
     6841 agtgccgctc ttactacatt aatcagggaa cgcccgccca gttgggaggt atgttggtgg
     6901 atatggcggc gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc tactcaagcc
     6961 ccaccgttac ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc
     7021 atcctggctg ggaaattgtc actacgcagt ttggctataa cgatgccact aaatcattac
     7081 aaaccgcaga aggaatatta aaagcgtata gcgatctcga cgccattatc gcccccgacg
     7141 ccaacgccct gcccgctgcc gcacaagccg cagaaaacct gaaaaatgac aaagttgcga
     7201 ttgtcggatt cagtacgcca aacgtgatgc gtccatatgt ggaacgcggc acggtgaaag
     7261 aatttggcct gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat
     7321 tattgaaaaa aggatcaatg aaaacgggcg acaagctgga tatccagggc gtaggtcagg
     7381 tggaagtctc gccaaacagc gttcagggct atgactacga agcggatggt aatggcatcg
     7441 tactgttacc ggagcgcgtg atattcaaca aagagaatat cggcaaatac gatttctgat
     7501 gtgcattact taaccggagt aagttatggc agatttagac gatattaaag atggtaaaga
     7561 ttttcgtacc gatcaaccgc aacaaaatat cccttttatc ctaaaaggtt gcggtgcgct
     7621 ggattgggga atgcagtcac gcttatcgcg gatatttaat ccgaaaacgg gtaaaaccgt
     7681 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
     7741 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
     7801 gcgcagcgta gttcccccag cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
     7861 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg acgccgtgcg
     7921 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
     7981 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
     8041 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
     8101 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
     8161 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
     8221 gcgcgaggcg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
     8281 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
     8341 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
     8401 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
     8461 gacaagattg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
     8521 gaaggcaatt tgcgcttcga tgtcttacag gacccagaag tgaattcgcg cttttatatc
     8581 tacgaagcct ataaagatga agacgcagtg gcattccata aaaccacgcc ccactacaaa
     8641 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     8701 gggttgatgc cgtgaggcga attcatcagt tttatctaca attggggtaa cgcgctgacg
     8761 ggagtaaata aatgtctgac tggaacccct ctttatatct gcactttgcc gctgaacgat
     8821 cgcgtccggc ggtggagctg cttgccagag taccgctgga gaatatcgaa tacgttgccg
     8881 atcttggctg tggcccaggt aacagcaccg cccttctaaa ccaacgttgg cctgctgcca
     8941 ggataacagg catcgactcg tctccggcga tgattgctga agcgcgcagt gctttgccag
     9001 actgccagtt cgtggaagca gacatccgca actggcaacc ggtacaggca ctcgatctga
     9061 tttttgctaa tgcctcactg caatggctgc ccgaccacta cgaattgttt cctcatctgg
     9121 tttctttact taatccgcag ggcgtgctgg cagtacagat gccagataac tggctggagc
     9181 cgacccatgt gctcatgcgc gaagttgcct gggaacaaaa ctacccggat cgcgggcgtg
     9241 agccgttggc tggcgttcat gcttactacg atattttgag cgaagccgga tgtgaggtcg
     9301 atatctggcg aaccacctac tatcaccaga tgccgtcaca ccaggcgatt atcgattggg
     9361 tgaccgccac tggattacgt ccgtggttac aggatctgac cgagagcgaa cagcagcttt
     9421 ttcttaagcg ctaccatcag atgctggaag agcagtatcc actgcaagag aacggacaga
     9481 tactgctggc atttccgcgt ctttttattg ttgcccggcg tatggagtaa atatcacgtc
     9541 agctggtaat gacgatcggg aagaattttt gctggaattt cggcttcatc gttcatctgt
     9601 aacaggtcaa tttcaatagc gttgcagatg gcatccagcg gtaaatcatt gttttcagta
     9661 ccgaacggat cttccagttc ttccgccaga caatccagcg aaataaaagt gtaggaaatc
     9721 agcacagaga taaaaggcgt catgtaatgc aggtccacga ccagcgcgaa cggcagcatg
     9781 atacaaaaca gataaacggt acgatgcaaa atcagggtgt aggcaaaggg aattggcgta
     9841 taggcaatgc gctcgcatcc tgccaggacc gctgaaatat cattaagccg atcgttgagg
     9901 ctaataaaca ggatatctga aagctgccca ttgcggcgct gaaccgccaa ccactccccc
     9961 attattaaca agatacggtt agctggagag ttcgaagcca gtacaggctg aagatcttca
    10021 gtcttgagat aatgagccag cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa
    10081 cagtgggcga aggcgatttg caaccgggca aactccctta cacttgccga atccggcaat
    10141 gtcgttttta cctcgcgcag taacgaccgt gaggcaatca tcaactgccc ccaaagtttt
    10201 cgcgcttcaa cgtaacgggc gtacccggca ttattacgaa aaccaagaaa aatggcgatg
    10261 gcgacaccga gaatgctgaa cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag
    10321 ggcagcatga aaataacagc gatagaaaag agaaaattga gtagtaagcg cgatgatatc
    10381 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
    10441 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
    10501 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
    10561 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
    10621 ccggtcgaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
    10681 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
    10741 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
    10801 gggtaccgat aacacggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
    10861 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aaggcaatca
    10921 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc ttttgccttc tgccctgcca
    10981 gcagctgcgg caggatgcga gtgcggaact tggtcatacc gttgagcgcg atagacagca
    11041 gctgatgctt aatgtacggg ttacggaaac gcccggtgac tgcactggcg aaagattcca
    11101 gttcatcacg aggcaaatcc agtaccggaa taatttcttc gtagatagct ttttcaacga
    11161 atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt atccagccct gcctggaacg
    11221 ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt tctttgtacg
    11281 gtttaatatc gtcaacaatc agcacgttga gcggatattt gtccagacgc agttcagtcg
    11341 ctaaggattt cggcccctga atcacaaaca ggtaaaagtg ttcagcggtg tcgaggaaac
    11401 catcgtgata acccagttct tcttccagtt tagccacttc atcgcgcgga taaccggtaa
    11461 cgatacggtc caccagcgta gaacagaagc tgttagcctg atccaaccat tgaataaatg
    11521 cttctggcag tgcccactct tgagcatagc gcagcaccag ttcacgcaac gcgtcgccat
    11581 tgtagtcgat caactcacac gggatgatga tccaaccttt atccagcgca ccgttgaaat
    11641 ggctaaaacg ttcgaacagc agacgggtca gttttgcggg atagcttacc gctggcgcgt
    11701 catcgaattt atcgcccgca tggtagctga tacccgcttc ggtggtgtta gagaaaacaa
    11761 agcgcatttc cggattgtgc gccagtttca ggaattcatc gtattcactg tagacgctga
    11821 tttcacgatt aacagagcga atcagacgcg cgtcgctgac cgcttccccc ttctcattca
    11881 ggccacggat aatggtggtg tacagaccat cctgcgtgct cagtgacggc gggaatgaag
    11941 tttcaatcgg acgaacaacg accacgccag aattcagatc ggtgtgctca ttcaggagat
    12001 cgatttgcca gtcaacaaag gcgcgcagga agttaccttc accaaactga atgatacgtt
    12061 ctggatactg tgcaccggga aaatcgcgac gatttagtgt tttcacaatg ggttcccttc
    12121 tgattagtca tacaacctgt ttgaattggt acgacaggtt agcaaacttt aatacgccga
    12181 acccctgttt tgatcaactc ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc
    12241 ggatcatggt ttaatcgagg aaatacgcct tttcctggat cataaagtgg tagaacacat
    12301 tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa cttcctcacc gtaacgcata
    12361 gtgctggtac ggttgcgccc atctttcttc gagcgataca gacacgtatc ggcttcgacc
    12421 atcaatttat taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact accgacgcca
    12481 atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct
    12541 ttacggattt tttccgccat tagcagacca tcgacaggat tcaccgacgg cactgcaaca
    12601 gcaaattctt cgccgcccat gcgcgccacc agccccttat caccgacaat cttctgaata
    12661 tgccgggcaa acacgcttaa cactttatcg ccacattcat gtccatagtt atcgttaatg
    12721 cttttgaaat agtcaatatc aagcagcatg accgtcagat gttgtgtctg tttcagcgat
    12781 ggacttttca gcgcctcata aagaccggac cgtgagtaaa cctgagtcag aaaatcaaag
    12841 tcggctcgca gcgcaacttg cttcattagc gaattgatcg ctgccacgct aaaagaaacc
    12901 ataattgggc atatcgccat cgtggcaata ccgagacgtg cggagaacat ttccggaatg
    12961 gagaacggcg aaccgaccga gatatcaatc accgaatttg ccaccagtac gatttccacc
    13021 gcaccggtga caaaggtcaa cagacatgtt acctgcggcg tatagcgcac tgcacaccag
    13081 attaatgcag gcagcgggaa cgccagactc cccgctccgc caatgactac cgaggcaata
    13141 accgacacaa taagcgcgat ggcgggcatc atctgttctg ctttaaagcg cggcagcact
    13201 ccaggaatag ccaacgtcag catacaaggc acgatcaaaa cgcccgttga gaattgctca
    13261 ctgaaccagt ccgcaagcaa aggccagaaa tccagactat caatactgac cgaaccaatc
    13321 gccccgacaa tagcgcataa taatgcggca atgagacagt aattaaatag ccgtaaggca
    13381 cttactggct catacttatt tttccccaga cgcttatccc gcacgaccag taaggcgacg
    13441 gtaacaataa acatcatatt ggagaaattg ataaccagtg aaaccagtcc ccattcggtg
    13501 gttatggcat cataaaccag catcgccaca taactgatcg catagtaatg cagtcgatta
    13561 agccagacat agcgggcaaa cacccccgcc attacgccat tcagcggcca gaaaagagaa
    13621 agttcgtgaa ccagccgtaa ctctgcacca ataaaataga acagcgtggt tagcgcaaaa
    13681 atagcgatgc tattacgtag cagatgacct tcctggaaca aacggaaagt tgaaatgggg
    13741 tgtacatgca ttaattatct gtccatacaa ttgcgggtgg caattatctc aacgtaataa
    13801 gtaaattctc ataaaggata gcatgttcgc taacagcggc ggcaaataaa ggtaccctgg
    13861 gaatgctatt ttatcgtttt ccgcaattct catggctgat tcatctgtac aaccagccag
    13921 tgatagatta ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac accctgcgga
    13981 atgttatgcc acttagccag acaaccgcga cagcaggtcg cggtggcgtg ctgagcgata
    14041 aacaccggat gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa tgccggagca
    14101 agacgtttag cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc tttctccagg
    14161 caatactgac gctctttcat gcccagacga aagcgagagc gaaattttga acgggataaa
    14221 cgcgcgaaga gtggatcgag agactgcatt aataaaccga acgccctaac tgtttcgtca
    14281 attgttcaag gacggcaata cccgcaagcg agttacctgc atcatccagt tccggactcc
    14341 agacagcgat ggccatttca tgcggaacaa tcgccacaat accgccacca acgccagatt
    14401 tcgccggtag ccccacccgc caggcaaact cccccgcgtt ctggtacata ccactggtcg
    14461 ccatcagcgc gttaatttgc cgcgcctgca ttggcgtcac cacaggttca tcaatatgaa
    14521 tagctttccc ctgattagcc agaaagacaa acgtccgggc cagctctaca cagctcattt
    14581 tcagagcgca gtaatgaaag tagttttgca gaacggttgt cacatcatga tggaaattgc
    14641 caaacgactt catcagccag gcgatagccg catttcgcgc ggaatgttca aattcggaac
    14701 gcgctaccac cgtatcgtat gaaatatcag acacaccgct taagccgcgc acgacttcca
    14761 gcatacgttg ccgtggtgcg cttaatcgcc cttgcaacat atcgcaaacc accagcgcac
    14821 cagcattaat aaacggatta cgcggaatac cctgctccat ttccagttgc actaaagaat
    14881 tgaacggtga tccagacgga tctttgccga cgcgttgcca gatttcctct tcggagtaat
    14941 gacgcatggc gacaacgaga ctcagcactt tggaaataga ctgaatggaa aaacgttctt
    15001 gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca gatagcaatg cccaatcggg
    15061 aaccgtctac tgtagccagc gccggaatat aatccgcgac tttaccctga ccaatgagcg
    15121 gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc catggcgact gccactttct
    15181 actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctgaat atgtcgcgtc
    15241 agatccggtc tttccacacc gtctggatat tacagaattc gtgtaagccg aaatgggaaa
    15301 gctcacgacc aaagccactc tttttcacgc caccaaaggc cactcgcgcg tcgctggcac
    15361 aataaccatt gataaacacc ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg
    15421 tttcgtcggt ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta gccagttcca
    15481 gcgcatgttc tgcatctttc gcaacggtga ttgccgcaac agggccaaac atttcttccc
    15541 gaaacgcggt catttctggg gtaacattcg ccagaaccgt tggcggatag tagttacctg
    15601 ccccagccat cttttccccg cccagtaaca aacgcgcacc ctgcgccagg gttttctcca
    15661 cctgatgatg cagctcatca cgtaaatcaa aacgagccat tggtccgaga gcgttctctt
    15721 catcacgggg atcgcccatt ttcaaggctg ccgcagctgc cacaaaacgt tcggtaaatg
    15781 cagaagcaat tccctcttcg ataataaagc gttttgccgc tgcacaaacc tgtccggtat
    15841 tctgataacg tccggctacc gccgctttaa ccgccagttc cagatcggca tcgttaagca
    15901 caataaatgg atccgaaccg cccagttcca gtacgcattt tttcagtgcc gctccagcct
    15961 gtgcgccaat agccgctccc gcacgaacac ttccggtcac cgtgacagca gcaatgcgcg
    16021 aatctttaat catttgactg acaccgtcgt tgtcggcatt cagccagcca tatacgcctt
    16081 gcgggattcc cgcatcttta aacacctggg caatgagctg agcacagccc atcacattcg
    16141 gcgcatgttt aagtaagtag ccgttacctg caagaatgat gggaaccgcg ccacgcatca
    16201 cctgccataa cggaaaattc cacggcataa tcgccagaat cgtccccaac ggtcgatact
    16261 caataaccgc ctgctgattt tccaccagcg taggttccgc cttcagcatt gccggaccat
    16321 gttctgcata ccagtcacac aaattcgccg atttcgccac ttcagcgcgc gcctggttga
    16381 ttggtttgcc catttcgcgg gtgatcattt gcgccatttc ttcgctacga gcgcgcagag
    16441 ccttaccgat atcacgcagt ttttcagcac gataatctat atttgtctcg cgccagtcgc
    16501 gaaagcctgc tgccgccagc tgaagtgcgt tttcgatatc gttagcgcca gcccacggca
    16561 gcacagaaag ttgttcaccc gtggcaggat ttattgaaat tgcatgagtt gccggagtaa
    16621 tggtcatcgg ggtatctcct ttatgagtca tggtatgaag atacgcagat ttactcttgc
    16681 tttaaaatga ataatattaa gccacttatt cacgaatcga gaatattatg gatctgaccc
    16741 aactggagat gttcaacgcg gttgccgaag ctggcagcat aacccaggct gcagcaaaag
    16801 tgcatcgcgt gccgtcgaat ctcactaccc gtctacgcca gctggaaaca gaactggggg
    16861 ttgatctgtt tattcgcgag aatcagcgtt tacgtctctc tcctgccggg cataactttt
    16921 tacgctatag ccaacaaatt ctcgcgttag tggatgaagc gcggagcgtt gtcgctggcg
    16981 atgagccgca aggtttattt tctcttggtt cgctggaaag caccgcagca gtgcgcattc
    17041 cggccacgct ggcggaattc aaccatcgtt atcccaaaat tcagttttca ctttccaccg
    17101 gcccttccgg cactatgctg gaaggagtgc tggagggaaa actgaatgcg gcatttattg
    17161 atgggcccat taaccatact gccattgacg ggataccggt ataccgcgag gagctgatga
    17221 tcgtcacgcc acaaggtcat gcgccagtaa tccgcgccag tcaggttaac ggcagtaaca
    17281 tttatgcctt ccgcgccaat tgttcgtatc gtcgccactt cgagagctgg tttcatgctg
    17341 acggtgccgc tccgggaact atccatgaga tggagtctta tcacggaatg ttggcctgtg
    17401 tggtcgcagg agcaggcatt gcgcttattc cacgctctat gctggaaagt atgccggggc
    17461 atcaccaggt tgaagcgtgg ccgttagctg agcaatggcg ttggttaaca acctggctgg
    17521 tctggcgtcg tggtgcgaaa acgcgtccgc ttgaggcatt tattcaacta ctggatgtgc
    17581 ctgactcggc aaaacagggt taccaatgag ctatttttga ta
//
LOCUS       DAFAVF010000085        17614 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFAVF010000085 DAFAVF010000000
VERSION     DAFAVF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.085"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            64..1203
                     /gene="arnB"
                     /locus_tag="LGK42_002828"
     CDS             64..1203
                     /gene="arnB"
                     /locus_tag="LGK42_002828"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBK2659057.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1207..2175
                     /gene="arnC"
                     /locus_tag="LGK42_002829"
     CDS             1207..2175
                     /gene="arnC"
                     /locus_tag="LGK42_002829"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBK2659058.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2175..4157
                     /gene="arnA"
                     /locus_tag="LGK42_002830"
     CDS             2175..4157
                     /gene="arnA"
                     /locus_tag="LGK42_002830"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBK2659059.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4154..5044
                     /locus_tag="LGK42_002831"
     CDS             4154..5044
                     /locus_tag="LGK42_002831"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBK2659060.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSSNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5044..6696
                     /gene="arnT"
                     /locus_tag="LGK42_002832"
     CDS             5044..6696
                     /gene="arnT"
                     /locus_tag="LGK42_002832"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBK2659061.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6693..7028
                     /gene="arnE"
                     /locus_tag="LGK42_002833"
     CDS             6693..7028
                     /gene="arnE"
                     /locus_tag="LGK42_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBK2659062.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7028..7414
                     /gene="arnF"
                     /locus_tag="LGK42_002834"
     CDS             7028..7414
                     /gene="arnF"
                     /locus_tag="LGK42_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBK2659063.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7408..7674)
                     /gene="pmrD"
                     /locus_tag="LGK42_002835"
     CDS             complement(7408..7674)
                     /gene="pmrD"
                     /locus_tag="LGK42_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBK2659064.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7784..9139)
                     /gene="menE"
                     /locus_tag="LGK42_002836"
     CDS             complement(7784..9139)
                     /gene="menE"
                     /locus_tag="LGK42_002836"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBK2659065.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9136..10098)
                     /gene="menC"
                     /locus_tag="LGK42_002837"
     CDS             complement(9136..10098)
                     /gene="menC"
                     /locus_tag="LGK42_002837"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBK2659066.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10098..10955)
                     /gene="menB"
                     /locus_tag="LGK42_002838"
     CDS             complement(10098..10955)
                     /gene="menB"
                     /locus_tag="LGK42_002838"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBK2659067.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10970..11728)
                     /gene="menH"
                     /locus_tag="LGK42_002839"
     CDS             complement(10970..11728)
                     /gene="menH"
                     /locus_tag="LGK42_002839"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBK2659068.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11725..13395)
                     /gene="menD"
                     /locus_tag="LGK42_002840"
     CDS             complement(11725..13395)
                     /gene="menD"
                     /locus_tag="LGK42_002840"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBK2659069.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13484..14779)
                     /gene="menF"
                     /locus_tag="LGK42_002841"
     CDS             complement(13484..14779)
                     /gene="menF"
                     /locus_tag="LGK42_002841"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBK2659070.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14858..15163)
                     /gene="elaB"
                     /locus_tag="LGK42_002842"
     CDS             complement(14858..15163)
                     /gene="elaB"
                     /locus_tag="LGK42_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBK2659071.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15218..15679)
                     /locus_tag="LGK42_002843"
     CDS             complement(15218..15679)
                     /locus_tag="LGK42_002843"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2659072.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSETLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15744..16661
                     /gene="rbn"
                     /locus_tag="LGK42_002844"
     CDS             15744..16661
                     /gene="rbn"
                     /locus_tag="LGK42_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBK2659073.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16849..>17614
                     /locus_tag="LGK42_002845"
     CDS             16849..>17614
                     /locus_tag="LGK42_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBK2659074.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSEDLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKC"
BASE COUNT         4162 a         4742 c         4442 g         4268 t
ORIGIN      
        1 tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc ggaaggaaaa
       61 gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga actcgctgca
      121 gttaaagagg ttctcgaatc cggttggctc acaaccggtc cgaagaatca ggcgcttgag
      181 caagcctttt gccagttgac gggaaatcag catgccatcg cggtcagttc agccaccgcc
      241 ggaatgcata tcacgctaat ggcgttggaa attggcaagg gcgatgaagt gattacgcct
      301 tccctgacct gggtttcaac cctcaatatg atttccttgt tgggtgcaac gccggtaatg
      361 gtggatgtcg accgcgatac gctgatggtc acgcctgaag cgatcgagtc agccataacg
      421 ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga tattgacgcc
      481 attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc ccatgccgtc
      541 ggtacgtatt acaaagggcg acatattggc gcaaaaggta ccgctatttt ttcatttcat
      601 gccattaaaa atattacctg tgcggaaggt ggcctgattg taactgataa tgaaaacctt
      661 gcccgccagc tacggatgct gaaatttcac ggtctgggtg tcgatgccta tgacagacaa
      721 acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg gctataagta caatctgacc
      781 gatattaacg ccgcaattgc cctgacacag ttagcaaaat tagagcacct caacacccgt
      841 cggcgcgaaa ttgcccagca atatcagcaa gcactggcag ctctcccctt tcagccatta
      901 agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg tgtcgatgaa
      961 caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaacg aggcattggt
     1021 accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg ttttcccacg
     1081 ctgtcgttac cgaataccga atggaatagc gaacgcatct gttctttgcc gctgttcccg
     1141 gatatgacta ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact cgcaggacaa
     1201 taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc cgtttataac
     1261 gagcaggaaa gcttaccgga attaatcagg cgcaccacca cagcctgtga atcgttgggg
     1321 aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc gcatatgctg
     1381 gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa ccgcaattac
     1441 gggcaacatt cagcgattat ggcgggattc agtcacgtta ccggcgactt aattattacc
     1501 cttgatgccg atctccagaa tccgccagaa gaaatccccc gcctggtggc aaaagccgat
     1561 gaaggttacg acgtggtagg gactgtacgc cagaaccgcc aggacagctg gtttcgtaaa
     1621 acagcttcga agatgattaa ccggcttatt cagcgcacca ccggcaaagc gatgggtgac
     1681 tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt gcactgccat
     1741 gaacgtagca cctttatccc gattctggcg aatatcttcg cccgccgtgc cattgaaatt
     1801 ccagtacatc attccgagcg tgagtttggt gaatccaaat acagttttat gcgcctgatt
     1861 aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat gctgagtctg
     1921 ctcggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt gattttacgc
     1981 ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc cgtgctgttt
     2041 acttttattg gcgctcagtt tatcggcatg ggattactcg gtgaatatat cggcaggatc
     2101 tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg tccatccagc
     2161 aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga tgcctcggta
     2221 ttgaagctct gctggctgcc ggttacgaaa ttagcgccat ttttacccat accgataatc
     2281 ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc agcggaaaga ggcattccgg
     2341 tttatgcgcc ggatgacgtt aatcatccgc tgtgggtgga acgcattgcc caactgtcgc
     2401 cagatgtgat tttctctttt tattatcgcc atcttatttg cgacgaaatt ttgcagctcg
     2461 ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc aaaatatcgt ggtcgcgcgc
     2521 cgctgaactg ggtgctggtc aacggtgaaa cggaaactgg cgttacattg caccgaatgg
     2581 tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg cattgccatt gcgccagacg
     2641 atatcgctat tacgctgcat cataaattgt gccatgccgc gcgccagcta ctggaacaga
     2701 cattacccgc cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa aacgaagcca
     2761 cctgttttgg tcgcagaacg ccggatgaca gtttccttga atggcataaa ccggcatccg
     2821 tactgcacaa catggtacgt gccgttgccg atccgtggcc gggtgccttc agctatgttg
     2881 gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc aaagcacagc
     2941 cggggagcgt gatttctgtt gcgccactgc tgattgcctg tggcgatggc gcgctggaaa
     3001 tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg ctcgcaatta gcgcagacgc
     3061 tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgcaccgcc cgacgccgta
     3121 cccgggtact catcctcggg gtgaatggct ttattggcaa ccatctgaca gaacgcctgc
     3181 tgcgcgaaga tcattatgaa gtttacggtc tggatattgg cagcgatgcg ataagccgtt
     3241 ttctgaatca tccgcatttt cactttgttg aaggcgatat cagtattcat tccgaatgga
     3301 ttgagtatca tgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata gccacgccga
     3361 ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag aatctgcgca
     3421 ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt cccgtcaact tcagaagttt
     3481 atgggatgtg tagcgataaa tacttcgatg aggaccattc taatttaatc gtcggcccgg
     3541 tgaataaacc acgctggatt tattcggtat caaaacaatt acttgatcgg gtgatctggg
     3601 cctatggcga aaaagagggt ttacagttca ccctcttccg cccgtttaac tggatgggac
     3661 cgcgactgga taaccttaat gcggcacgaa tcggcagctc ccgcgctatt acgcaactca
     3721 ttctcaatct ggtagaaggt tcaccgatta agctgattga tggcggaaaa caaaaacgct
     3781 gctttactga tattcgcgat ggtatcgagg cgttataccg cattatcgaa aatgcgggaa
     3841 atcgctgcga cggtgaaatt atcaacattg gcaatcctga gaacgaagcg agcattgagg
     3901 aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat catttcccac
     3961 cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga tatcaggacg
     4021 tagagcatcg taaaccgagc atccgcaatg cccaccgctg cctggactgg gagccgaaaa
     4081 ttgatatgca ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc gttgatctta
     4141 cggataaacc atcatgacca aagtaggctt acgcattgat gtcgatacct ttcgtggcac
     4201 ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag cataatattc aggccagcat
     4261 ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg tgaagccaca
     4321 gtttttgtgg aagatgctgc gctcaaatgc ggcatcgctt tatggctggg atattttact
     4381 ggcaggtacg gcctggccag gtaaagagat tggtcatgcc aatgccgata tcattcgtga
     4441 agcggctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct ggcaagcccg
     4501 tagcagtaac tgggatcggc aaacaatgat cgacgatatt gcacgcggtc ttcgcactct
     4561 ggaagagatt atcggtcaac cggtaacctg ttctgccgct gcgggctggc gtgccgacca
     4621 gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt gtcgtggggc
     4681 catgccgttc cgtccattgc tcgaatcagg aaaccctggc actgcgcaaa ttccggtgac
     4741 cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt ttaacggttg
     4801 gttactcaac cgcatcctgc gagataaagg cacgccggtt tataccattc atgcagaagt
     4861 tgaaggctgc gcttatcagc ataattttgt ggatctcctc aaacgcgcag ctcaggaagg
     4921 cgtgacattt tgccctttaa gcgaactgtt atcagagacg ttgccgctcg gacaagttgt
     4981 tcgcggaaat attgccggac gtgaaggctg gctgggttgc caacaaattg cgggtagtcg
     5041 ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg ctattacctg
     5101 ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa cgcgttatgc ggaaatcagt
     5161 cgagaaatgc tggcatccgg cgactggatt gtgccccatc tgttagggct acgttatttc
     5221 gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt tggcgcgaat
     5281 aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc gctggtgacc
     5341 tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccac agtaatttat
     5401 ctctcattgt ttattgtcta tgccatcggc acttatgccg tgctcgatcc gtttattgca
     5461 ttctggctgg tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca gacgtggaaa
     5521 ggcaaaagcg caggattttt actgctggga atcacctgcg gcatgggggt gatgaccaaa
     5581 ggttttctcg cccttgccgt gccggtatta agcgtgctgc catgggtagc gacgcaaaaa
     5641 cgctggaaag atctctttat ttacggctgg ctggcggtta tcagttgcgt actgacggtt
     5701 ctcccttggg gactggcgat agcgcagcgg gagcctgact tctggcacta ttttttctgg
     5761 gttgagcata ttcaacgctt tgcactggat gatgcccaac atagagctcc gttctggtac
     5821 tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg gattactccc cggtgcactg
     5881 tacacaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag ctggacgata
     5941 atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc ccacctatat tctttcctgc
     6001 tttgcatctc tggcaatgct gatggcgcat tacgctttgc tggcagcaaa aaataatcct
     6061 ctggcgctgc ggattaatgg ctggattaac atcgcttttg gcgtcactgg cattattgcc
     6121 acatttgtgg tctccccgtg gggaccaatg aacacgccgg tgtggcaaac cttcgagagc
     6181 tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg ctggtacacc
     6241 ttaacaaacg tcgaaaagac ctggcctttt gccgcgcttt gcccgctggg gctggcgttg
     6301 ctggtaggat tttcaattcc tgacagagtt atggaaggaa aacatccgca attttttgtc
     6361 gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag cgtcggtgtt
     6421 gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg ccagacaggt
     6481 gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag cggtgatgag
     6541 ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttactctcgt gctttcggtt
     6601 gaccgcgatg aagatatcaa cagtctcgcc attccgcccg cagatgccat cgatcgtcag
     6661 gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta gtctttgcca
     6721 gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt gtggcgataa
     6781 acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt cttggtcttg
     6841 ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc tacccgatgt
     6901 taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac gaaccggtat
     6961 cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg cattgtgatc ctcgggagta
     7021 cggtgtaatg ggcctgatat ggggattatt cagcgtcatt attgcctcag ttgcgcaatt
     7081 aagcctgggt tttgcggcaa gtcatctgcc gccgatgacg cacttatggg attttatcgc
     7141 ggctctgtta gcttttggcc tggatgcccg gatactgtta ctcgggctgc tgggttatct
     7201 gctttccgtt ttttgttggt ataaaacgct acataaactt gcgctaagca aagcctatgc
     7261 cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc ccggctggga
     7321 agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg ggttgatgct
     7381 gattttcctg cccacgacaa aacaacgtta ctgagttttc cctgccgctg tacactgccg
     7441 ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat gcagcttttc
     7501 acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca ctttcacggc
     7561 gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc agagcataag
     7621 tacatgtctg ttatcttgtt tattgcagca cgattttttg accagccatt ccattgcatt
     7681 atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac tctttatcaa
     7741 cggcaatgga atggcagaaa tgagaagtga aacggagggt taattattgc tgacgttgca
     7801 cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagc tccggcggta
     7861 aagtcagcca gcgcaccggt tgttgaaaac gagccagctt atctttcacc cattcgccaa
     7921 gatcaacggt ttgctggtca tactccacca ccgctaccgg ccgatgacca aactccttgt
     7981 cggcaacggg aacgataaac acctgcaaaa ccgcaggatg tgcagcaata acacgctcga
     8041 cttcttccgg ctgaataccc tcaccgccac tgaagaacag attgtctaaa cgtccgacaa
     8101 tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag ccttcgtcat
     8161 taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg gcagcccgca
     8221 gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa ccaacgtctg
     8281 ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga ccatagccgc
     8341 aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg atagccgcgc
     8401 cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa cgccagagtt
     8461 gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat ggctgtttat
     8521 cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc tgaccggaga
     8581 cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga atcaacgata
     8641 acaccccttc ggcactggca agatgggcct gataagtatg gacagcggct ttcggcaacc
     8701 cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac gtagcggcat
     8761 gtgcgccttc aacccgctga atgtgcagcg acgttaacgc cggaaacgtg ttttccccat
     8821 ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc aacggttgcg
     8881 gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc agccaggcga
     8941 gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca acaacgccct
     9001 gcgccgcaaa gcctgaggct aattcatcga cgcgagcaca aagctcgcgc cagttaagtt
     9061 gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag tgacgccacg
     9121 gccagtcaga gaagatcata acaaccgctc cagtgcatca acttccacga caggcagcgt
     9181 gctacccggc cagcgacgta cctgctgcgc ctgcatcaga tccagcgtgt ccagccctgg
     9241 aatggtgtcc ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc ctaagctcga
     9301 ttcaatggaa gaactgatca ccgccgtcag ccccagcgcg tgcgccgcct gtacctgctc
     9361 gcgtactttc tccagactgc ccgtgagcgt gggtttgata actaccgcgc gcacgccctc
     9421 ttcagccaca aaggcaaaat ccggctcgcg caggctttca tcccaggcaa tggcaatgcc
     9481 ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt cgagaaacgc
     9541 gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctggcctt tcagcggtgt
     9601 ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca gcaacagatt
     9661 aaccaccatg ccgtcgcgca cggcttcgta caatcccact ttgaccttcg ccactttctc
     9721 gcctggcata tcagcaagtt tgaggatcag atcgtcagga tcgccattac acagtggtgc
     9781 cgcacggtaa ttggccgcct gcgggagcgt atctgccagc tccgccaatg cacagcttac
     9841 gccaaaggca acggaaggca tctgcggtag ctcgcaatcg cctgccagcc agttatttac
     9901 ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc ccggcagtgg
     9961 ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca gcccgtcgcg
    10021 ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct gccagcggta
    10081 tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg ctgacgtttc
    10141 tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag catggtggcg
    10201 ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag cgcagctttc
    10261 aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg gacggtttct
    10321 ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag cgcctgtttt
    10381 gcgtcgtact gacggcacag gaaccagatt tctcgcgctt ttttctgccc gacgatacga
    10441 gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc agtctgaccg
    10501 aagatggcat tatctgctgc gatagtcagg tcgcacatca tgtgcagaac gtgaccaccg
    10561 ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg gatctgacgc
    10621 tggaagtcca gcacgttcaa gtgatgtacg ccggaatcat ctttatagcc gccgtaatca
    10681 ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc agtcagaatg
    10741 atcacgccga tattgtcgtc ataacgcgca tctgccagtg cctggatcat ctcttttacc
    10801 gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt tgcgataccg
    10861 tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg ccattcaacc
    10921 ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt cagaaacgca
    10981 agatctgcgc cagacttgcg ataacgccag cgggattttc ccgatgcgcg ttatgtccgg
    11041 cgcgaggaat gacatggcag tcggcagcca gttccgccgc cagggcgcgg aatttgctgt
    11101 cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta gcacgtaaat
    11161 caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg ccattattgt
    11221 tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca aaaacaggct
    11281 gttgatacca gtcggcaaat accgccgtta acggttctgt gcgaaaacgc tgcgcccatt
    11341 ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc ggatgcccgc
    11401 cttcgacaat aaccccacaa agccccgcca gcccctggca agccgccatc atcgccaccc
    11461 gtccgccaag cgagtacccc accagccaaa agtcaaggat gttgtaacta accaaggttt
    11521 tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc gaaccaccat
    11581 gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc acttcttgcc
    11641 attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa cctggttttc
    11701 cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa gttgctggag
    11761 cgtttgcgca ccatcggtgt cgttaaccac catttcaatc accgtggtgg ttggcgtgcg
    11821 ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt tgcggacgat gatatttcag
    11881 ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat agaaacgctc
    11941 acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc ccgccgttgt tgttcaccac
    12001 aattaatacc agcggcgcag aaacctgacg caataacgcc agcgcgttga gatcgtaaag
    12061 tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct gaacgcctgc
    12121 ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg ttgctgtaca ccgggtaacc
    12181 tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac caacaaacaa
    12241 ttgcccctgt tcaggcagat agtcgcagat gcgatgcgcc agttgcgctt cgccaaacgc
    12301 atcacggcgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga tttcaacgca
    12361 ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat tggcaattaa
    12421 gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa caatccagta
    12481 ctcttctggt tcacagcttg cctgccattg caggagccgt ttgcccgtca ggctgcttcc
    12541 cagttgcacc acaatttgcg cctgctgcag ctcgctggtc gctttggcat tgcctaacca
    12601 aagatcggca cacggcagcg gctgcccggt ttgtgacagc acatcgccaa tcagcggcca
    12661 gccaagagtt tgcgcccaca gggcaacttt tttgccctct tccgcactca tgcgcccggc
    12721 aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt cactttccag
    12781 acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcaccca gacgctgttg
    12841 ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg ggcagttgat
    12901 atggactccc cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa ccagccaacg
    12961 tgcggggata tcccgggtcg ggcgcggcaa tgaaatactg tgcgtggggt gagaggcgaa
    13021 cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg gcgggcgatc
    13081 ggcggttaac aggatcagtt tttctccggt taacccagct tcaatcagtg ccggatagag
    13141 atttgctacc gccgtgccgg aggtcacaat caccgccacc ggctgcttgc tgactttcgc
    13201 cagccccagc gccagatgtc ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgt
    13261 ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg cgatacagat
    13321 gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa
    13381 tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata aggagctaca
    13441 aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc atctgtaata
    13501 aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg gggtcggaac
    13561 cacggacaat gcccgcgcca gcatataatc gcacgacatt gccgctaatt tttgctgagc
    13621 gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa ccggcgtacc
    13681 attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg cgcggtaagc
    13741 cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc gctttgttga
    13801 gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt acctgcggcg
    13861 gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct tccaccacca
    13921 gcatgttctc gcgctggttt ttatcatccg ccatcagcca ctctcctaac tgctgcgcct
    13981 gcttatcatc aggattattt gctactgttc ccgccagcgc ttcagtacgc agcgctttgt
    14041 cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg ccatcaaagg
    14101 ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc atcgccgccg
    14161 cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact ttgtcgagct
    14221 caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc gttttgtccg
    14281 gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg atactcacca
    14341 gtgtggcgat aaattctttt gcctgaatcg catcgtgctg aagggagctt tcgctgaata
    14401 gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgg ggtaaaagta
    14461 aattgccctg cgacgggtca aaagcattca gcccccaaat gcgtaagtcg gcgtgttccg
    14521 ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca
    14581 ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg tacgtttgct
    14641 gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga atatcgataa
    14701 cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg
    14761 ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt atacggggta
    14821 ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc agcaacagtc
    14881 ctagtaccag cccaacggcc gcgcccacac caattccttg ccagggtttt tcgtggacgt
    14941 agtcatcagc acgataaact gcctgcttcg cccgatagta ataactgtct gaagcctggc
    15001 taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc tcaacatatt
    15061 tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca ctaagcagcg
    15121 tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt ctccatttgc
    15181 gtaaaacctg tcactaacta tagacaaggg ttgttgatta cgcctgaatt acctcgcgtg
    15241 ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg ataaaaccga
    15301 aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc ttatcaggcc
    15361 agtgatgcgt acatgtttcg agtgtttcac tcatcagctg ctgccccact ttttcgccac
    15421 gcaacgcttc actgacaatc acccgaccta taacgaccgg ctcaagatca tcatcacttt
    15481 tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata tggcgattat
    15541 cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg acaacaaaca
    15601 ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca gagtgatgca
    15661 gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac tagccggaat
    15721 attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca gccggtgtgc
    15781 caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg acccagagcg
    15841 gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat accgccttta
    15901 accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat ctttttggtt
    15961 tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta acgatttatg
    16021 gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg gattagcggc tcatggaccg
    16081 attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat ggcctgcgca
    16141 aagtaaccgc ttatccgctg gaacacccac tggaatgtta tggctatcgt attgaagaac
    16201 atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg ccgcctggcc
    16261 cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga aggcagatta
    16321 acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt ttcggcgata
    16381 ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag
    16441 cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc tctacacgcc
    16501 aggctgcgac actagcccgt gaggctggag tcggtaagct aatcattacc cacgtcagct
    16561 cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgcaggtca attttcccgg
    16621 cgactgaact agcgaatgat ttcaccgtgt ttaacgttta actctgctta ttattaaaaa
    16681 cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc acacgggcat
    16741 attgagcgat ttttgatatt tattcatctc ctcacgcact atattcagag caataagttg
    16801 cctctattta tcttgattca gcacaacctt tgcaactaaa aggagcccat gatggttaca
    16861 gttgtcagca attattgtca attatctcaa acgcaactca gtcagacatt tgcagaaaaa
    16921 tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc cggagatgaa
    16981 gaaagcattg agttactgca taatattgcg ttaggctatg atgaatttgg aaaaaaagct
    17041 gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc gattatccga
    17101 cttatcaaaa atgcttgttt aaaattatat aatttggcac atgccgcaac caactccccc
    17161 cttaaaccag ctggtccgga taattcagag gatctcctgt tcaaaaagct attctcccct
    17221 tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa acagtcgctt
    17281 tcgaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa cttctgggat
    17341 aaaaatcgtc aattaaccac agatgaaata gattgctatc ttcagaagat cgccgctaat
    17401 gcaaaaaata ctgaagtcaa ttatcctact ggtctctacg tcccctactc caccagaact
    17461 cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc gaaagcagtc
    17521 cagttattcc ccataaatac tggcggacac tggatattag tttcgctaca gaaaatagta
    17581 aatgaaaaaa ataatacaca acaaataaaa tgcg
//
LOCUS       DAFAVF010000086        17249 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFAVF010000086 DAFAVF010000000
VERSION     DAFAVF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.086"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            49..633
                     /locus_tag="LGK42_002846"
     CDS             49..633
                     /locus_tag="LGK42_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2659075.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            713..1408
                     /locus_tag="LGK42_002847"
     CDS             713..1408
                     /locus_tag="LGK42_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2659076.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1438..3954
                     /locus_tag="LGK42_002848"
     CDS             1438..3954
                     /locus_tag="LGK42_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2659077.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKRGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDAKGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3965..5056
                     /locus_tag="LGK42_002849"
     CDS             3965..5056
                     /locus_tag="LGK42_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2659078.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFNITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5099..5959)
                     /gene="rsmI"
                     /locus_tag="LGK42_002850"
     CDS             complement(5099..5959)
                     /gene="rsmI"
                     /locus_tag="LGK42_002850"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK2659079.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6023..8059
                     /gene="lpoA"
                     /locus_tag="LGK42_002851"
     CDS             6023..8059
                     /gene="lpoA"
                     /locus_tag="LGK42_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBK2659080.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGCTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8017..8412
                     /locus_tag="LGK42_002852"
     CDS             8017..8412
                     /locus_tag="LGK42_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBK2659081.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8432..9022
                     /gene="diaA"
                     /locus_tag="LGK42_002853"
     CDS             8432..9022
                     /gene="diaA"
                     /locus_tag="LGK42_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBK2659082.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9032..9607
                     /gene="yraP"
                     /locus_tag="LGK42_002854"
     CDS             9032..9607
                     /gene="yraP"
                     /locus_tag="LGK42_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBK2659083.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9721..10761)
                     /locus_tag="LGK42_002855"
     CDS             complement(9721..10761)
                     /locus_tag="LGK42_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK2659084.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10834..11469)
                     /locus_tag="LGK42_002856"
     CDS             complement(10834..11469)
                     /locus_tag="LGK42_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK2659085.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11597..12115
                     /gene="yhbO"
                     /locus_tag="LGK42_002857"
     CDS             11597..12115
                     /gene="yhbO"
                     /locus_tag="LGK42_002857"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBK2659086.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12095..12538)
                     /locus_tag="LGK42_002858"
     CDS             complement(12095..12538)
                     /locus_tag="LGK42_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBK2659087.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12589..12891
                     /gene="yhbQ"
                     /locus_tag="LGK42_002859"
     CDS             12589..12891
                     /gene="yhbQ"
                     /locus_tag="LGK42_002859"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBK2659088.1"
                     /translation="MTPWYLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(12878..13381)
                     /locus_tag="LGK42_002860"
     CDS             complement(12878..13381)
                     /locus_tag="LGK42_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2659089.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13375..13899)
                     /locus_tag="LGK42_002861"
     CDS             complement(13375..13899)
                     /locus_tag="LGK42_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBK2659090.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPIKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14108..15103
                     /locus_tag="LGK42_002862"
     CDS             14108..15103
                     /locus_tag="LGK42_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK2659091.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15112..15990
                     /locus_tag="LGK42_002863"
     CDS             15112..15990
                     /locus_tag="LGK42_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK2659092.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16196..17203
                     /locus_tag="LGK42_002864"
     CDS             16196..17203
                     /locus_tag="LGK42_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBK2659093.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4353 a         4555 c         4362 g         3979 t
ORIGIN      
        1 attcacatta aatatgatta tgtacttgtt acaaggataa ggttataaat gaataaagtt
       61 acaaaaacag ctattgtggg cttacttgcg cttttcgcag gtaatgccgc tgcaaccgat
      121 ggtgaaatag tttttgatgg cgagatttta aaatccgctt gtgaaatcaa tgactctgat
      181 aagaaaattg aagttgctct tggtcactat aatgccgaac agtttcgttc tgttggcgat
      241 cgtagcccaa aaattccatt cactattcct ttagttaact gcccagtgac tgggtgggag
      301 cacgataacg gcaacgttga agcgtctttc cgtttatggc tggaaacgcg ggataatggc
      361 accgtcccta acttccctaa tctggcaaaa gtgggctctt ttgctggcac agcggcaaca
      421 ggtgtgggta tccgtattga cgatgcggaa agcggaaacc ttatgccact gaatgctatg
      481 gggaatgata acacggttta tcagatccca gcggactcca atggtattgt caatgttgac
      541 ctcatcgctt actacgtatc aaccgttgaa gcatcagaaa tcaccccagg ggaagcagac
      601 gctgtcgtta acgtaacgct ggattaccgt taatacgtta cggcgttatc tgacctttca
      661 gataacgccc ttttccttcc tctttctcgt tgtatcaggt tgaaaaataa ctatgtcaaa
      721 acgaacattc gcggtgataa taaccttgtt gtgtagcttc tgtatcggcc aggcgcttgc
      781 agggggtatc gttttacagc gaacgcgagt gatctatgat gccagccgca aagaggctgc
      841 gttacctgtc gcaaacaaag gcgcagaaac gccttattta ctgcaatcat gggtagataa
      901 tatagatggt acaagccgtg ccccatttat tataacccca ccgctatttc gtcttgaagc
      961 tggcgatgac tcatcactgc gaattattaa aacagctgat aacctgcctg aaaataaaga
     1021 gtcgctgttc tacattaatg ttcgtgccat tccagcaaag aaaaaatcag atgatgttaa
     1081 tgctaacgag ttgacgctgg tatttaaaac acggatcaaa atgttttatc gccccgcaca
     1141 cctgaaggga cgggtaaacg atgcgtggac atcactggaa tttaaacgta gtgaccattc
     1201 actcaatata tataacccaa ctgaatatta cgtcgtattt gccggactgg cagtcgataa
     1261 aaccgatctc acaagcaaaa ttgaatatat cgcgcccgga gaacataaac agttaccact
     1321 tcctgcatct ggcggaaaga acgtgaaatg ggctgcaatc aatgattatg gcggcagttc
     1381 cgggacagaa actcgtccac tgcaataaca aatataaaaa acacaggtca tcagggaatg
     1441 ccacaacgac accaccaggg acataaacgc acaccgaaac agttggcgct catcatcaaa
     1501 cgctgtttgc cgatggtgct cactggcagc ggcatgcttt gcactaccgc taacgccgaa
     1561 gagtattatt tcgaccccat tatgctggaa actacaaaaa gaggtatgca aacaactgat
     1621 ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt tgatatctgg
     1681 ctgaataaaa agaaggtttc acagaaaaaa attacattta ccgccaatgc agagcaactt
     1741 ctgcagccac agtttacggt agaacaacta cgtgagctgg gtattaaggt ggatgaaatc
     1801 ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca aatcattccc
     1861 ggtacagctg ctgaatttga tttcaatcat cagcgactta atttgagcat tccccaaatt
     1921 gcactgtacc gtgatgcaaa aggttacgtc tccccttctc gttgggacga tggtatacca
     1981 acgctgttta ccaactactc gtttacaggt tctgataacc gttaccgcca gggcaatcgt
     2041 agccaacgac agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg gcgattacgt
     2101 aactattcta cgtggacacg caacgatcag gcgtcaagct ggaacactat cagtagttat
     2161 ttacaacgtg atatcaaggc gttgaagtct cagttgcttc tgggagaaag cgccaccagc
     2221 ggcagtattt tttccagcta cacctttact ggcgtgcaac tcgcttccga cgataatatg
     2281 ttgccaaaca gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa cagtagtgca
     2341 atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc agcgggtgcc
     2401 tttgaaatta acgatctcta cccctcttcc aacagcggcg atttagaagt cacgattgaa
     2461 gaaagtgacg gtacgcaacg tcgctttatc cagccttatt cttcattacc catgatgcag
     2521 cgacctgggc atctaaaata tagcgcgacc gctggacgct atcgcgctga tgcaaacagt
     2581 gatagcaagg aacccgaatt tgctgaagcc acggcaatat atggtttgaa taatactttt
     2641 acgctgtatg gcggcctgct cggttctgaa gattattatg cgctggggat cggtatcggc
     2701 ggcacacttg gcgcactggg cgcgttgtcg atggatatca acagagctga cacccaattc
     2761 gataaccggc actcttttca tggctatcaa tggcgtacgc agtacatcaa agatatcccg
     2821 gaaaccaaca ccaatatcgc tgttagctac tatcgctata ccaacgatgg ctattttagt
     2881 tttgatgaag ccaatacccg taattgggac tataacagtc gccaaaaaag tgaaattcaa
     2941 ttcaacatca gccagacaat atttgatggg gtaagtctgt atgcctccgg ttcgcagcaa
     3001 gactattggg gcaataacga taaaaacagg aatatctcag ttggggtttc cggtcagcaa
     3061 tggggaattg gttacagcct gaattatcaa tacagtcgct acacggatca aaataatgac
     3121 cgcgcactct ctttgaatct cagtattccg ttagaacgct ggttaccgcg tagccgggtt
     3181 tcctatcaga tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg tcttgatggc
     3241 tcactgctgg atgatggtcg cctgagctat agcctggaac aaagtctgga tgacgataac
     3301 aaccataaca gtagcctgaa cgccagttac cgttcacctt atggcacctt cagtgccgga
     3361 tacagctacg gtaatgacag cagccaatac aattacggcg ttaccggcgg cgtggttatc
     3421 catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct tatcgatgct
     3481 aatggggctt ctggcgtgag gatacaaaac tatccgggga ttgctactga tccctttggc
     3541 tatgcagtgg ttccttatct cacgacttac caggaaaacc gtctctcggt agatactacg
     3601 cagctgcccg ataacgtcga tcttgagcaa acaacacagt ttgtggtgcc caacagaggt
     3661 gcaatggtag cggcgcgttt caacgccaat atcggttatc gcgtacttgt tacagtcagc
     3721 gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca gcaacgatga gacggggcaa
     3781 caaagtatcg tcgatgaggg cggcatacta tatctctctg ggatatcgag taaatcacaa
     3841 agctggactg tacgctgggg aaatcaggca gatcaacaat gtcagtttgc ttttagtaca
     3901 ccggattcag aaccaacaac ctctgtatta caaggcacgg cgcagtgcca ttaaggataa
     3961 aaaaatgaaa agagcgcctc ttataacagg acttttgttg atatccacat cctgcgctta
     4021 tgcctcctca ggagggtgtg gagctgacag cactagcggt gcgacaaatt acagcagtgt
     4081 ggttgatgat gttacggtga accagacaga taacgtgaca ggacgggagt ttacctctgc
     4141 cacgctaagt agcactaact ggcaatacgc ctgtacctgc tctgcgggta aggcagttaa
     4201 acttgtctat atggtcagcc ctgtacttac caccactgga catcaagcag gatattacaa
     4261 actcaatgat agtctggata ttaaaaccac attacaggcg aatgacattc caggactcgt
     4321 gactgaccag accgtttctg ttaacacccg attcacacag ataaaaagca acaccgtata
     4381 ttctgctgca acccaaacgg gtgtttgcca gggtgacacg tctcgttatg gacccgttaa
     4441 tattggtgca aataccacct ttaccctgta tgtcaccaag ccatttctcg gctcgatgac
     4501 cattccgaaa acggatattg ccgtcattaa aggcgcatgg gtcgatggaa tgggaagccc
     4561 gtctacaggt gacttccatg atttagtcaa gttatcgatt cagggaaatc tcaccgcccc
     4621 acagtcgtgc aaaattaatc agggcgatgt tattaaggtt aattttggat tcatcaatgg
     4681 tcagaagttt accacccgca atgccatgcc agacggtttt actccagtag actttaatat
     4741 cacttatgac tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc gcatcgacgg
     4801 tacaactggg gtagtagacc agtacaacct ggtcgccaga cgaagaagtt cagacaatgc
     4861 gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga gttgcaaata ttccttttca
     4921 gaacggtatc cttcccgttg atccttccgg gcatggcacc atcaacatgc gcgcctggcc
     4981 agttaatctg gtcggtggtg agctggaaac aggaaaattt cagggcaccg ccaccattac
     5041 cgtcatcgtg cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt cggcactctt
     5101 acccctgctg ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatttctg
     5161 ccgccagcgc cgccgctttt ttcaacggca gttctgcctg tagcagcgcc agcgtgcgca
     5221 gggcatcggc gggtaagtct tcttcctgtg ctttatgacc ttcgataatc agcaccattt
     5281 cgcctttgcg acggttttca tcttctttta cccacgccag cagctcgcca acgggcgcgc
     5341 cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag aaccacgtag cgggattcgc
     5401 ctaataccgc aacgatatct tccaggctat ctaacagacg gtgggtggat tcataaaaaa
     5461 tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc acggcggcct tttgatttgg
     5521 caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa acccgcggcg cttaacgcag
     5581 tgatagcagc acacggcccg ggtagcggca ccacgcaaat ccccgcttca cggcaggtac
     5641 gcaccagatg gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa
     5701 tgttctgccc ttcttgcagc ttcgccagca gcgtttcggc tttttgttgt tcgttatggt
     5761 cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac
     5821 gagtatcctc ggcggcaatc agatcaacgg cctgtaatac ctctaacgca cgctgggtga
     5881 tatccgccag attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg
     5941 ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta
     6001 aaaaaatatc actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg
     6061 ttgtctgcct gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga
     6121 tcagtccact gcttatatgc agggcacggc gcaggctgat tctgcctttt atcttcagca
     6181 gatgcagcaa agctctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact
     6241 ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac cgcaagaact
     6301 gaacgattct cagcgtcgcg agaaaacact gctggcggta gagattaaac tggcgcagaa
     6361 agattttgct ggcgcgcaaa acttgctggc gaaaatcaca cctgccgatt tagaacaaaa
     6421 ccagcaagcg cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga
     6481 tttactgcgc gcgttaattg ctcaggaacc gctgctcggc gcgaaagaaa aacagcagaa
     6541 tattgatgcc acctggcagg cgctctcctc catgactcag gaacaggcga atacgctggt
     6601 gatcaacgcc gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga
     6661 taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc gactggcaaa aacgttatcc
     6721 gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc
     6781 agcctcgacc aacaaaatcg ccctgctgtt gccgctgaat ggccaggcag cggtatttgg
     6841 ttgcactatt cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcggc
     6901 tcaggtagct gccgcacctg ccgcagacgt agctgaacaa cctcagccgc aaaccgtgga
     6961 tggcgttgcc agcccggcac aagcctcggt tagcgatctg accggtgaac agcctgcggc
     7021 acagccagcg cctgtaagca ccccggcagc aaccaccaca gcggtaagcg cgccagcgaa
     7081 tccatccgca gaactgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag
     7141 ccaggttcag caggatggcg cgagtattgt ggttggtccg ttgctgaaaa ataacgttga
     7201 agagttgctt aagagcaaca ccccgctgaa cgtgctggca ctgaaccagc cggagaatat
     7261 cgaaaaccgc gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc
     7321 agcgcgtcat attcgtgacc agggtaaaca agcgccgctg gtgctgatcc cacgcagttc
     7381 attgggcgat cgcgtagcca atgcgtttgc gcaggagtgg cagaaactgg gcggcggcac
     7441 cgttctgcaa caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc
     7501 tggtatcgct ttaacgggta ccccgattac tcccagagcg acaaccgact ccggcatgac
     7561 gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac cagttcacca ataatggcgg
     7621 tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat
     7681 gatcgccatg cgtaacggta gccagagcgg tgcaacgctg tacgccagct cccgcagtgc
     7741 gcaagggacc gctggcccgg atttccgact ggagatggaa ggcttgcagt acagcgaaat
     7801 cccgatgctg gcaggcggta atctaccgtt aatgcagcag gcactcagcg cggtgaataa
     7861 cgattattca ctggctcgca tgtatgcgat gggcgtcgat gcctggtcgc tggcaaatca
     7921 tttctcacaa atgcgccagg ttcagggttt tgaaatcaac ggtaataccg gaagcctgac
     7981 ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg ctacagtacc aacaaggtca
     8041 ggtagtcccc gccagttaac caccaaacag accggcgatg cgtgggaagt acaagcgcgt
     8101 cgctggctgg aaggcaaagg actgcgattt gtcgccgcta acgtgaacga gcgtggcggc
     8161 gagatcgatc tgataatgcg tgaaggccgg accaccgttt ttgtcgaggt acgctatcgc
     8221 cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta
     8281 ttacagactg cccgcttgtg gctcgcgcgt cataatggga gttttgatac tgtggattgc
     8341 cggttcgatg tggtagcctt caccgggaat gaggttgagt ggattaagga tgcctttaat
     8401 gaccactcat aattaaggtt taaggattag cgtgcaagaa agaattaaag cttgcttcac
     8461 tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt ccggatgcca tctcccgtgc
     8521 agccatgacg ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg
     8581 aacttccgct gccaatgcac agcattttgc tgccagcatg atcaaccgtt tcgaaacgga
     8641 gcggcccagc ttacctgcca ttgcactaaa tactgataat gttgtcttaa cggcgattgc
     8701 caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg
     8761 agatgtattg ttagccattt ccacccgtgg caacagccgc gatattgtaa aagcggtaga
     8821 agctgccgtt acgcgtgata tgactattgt ggcattgacc ggctatgacg gcggcgaact
     8881 tgcaggtttg ttagggccac aagatgtgga gatccgcatt ccttcgcatc gtagtgctcg
     8941 cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct
     9001 tttccctcac caggatgttt aaggagaata catgaaggca ttatcgccaa tcgcagtcct
     9061 tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta ccgctgctgt
     9121 gggtaccaaa gccgcaactg acccacgcag tgtcggcacc caggtggacg atggtaccct
     9181 ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag attaagaaag aagcccgtat
     9241 taatgtcacg gcgtatcagg gcaaagtgct gctggttggg cagtcaccaa acgctgaact
     9301 ttcggctcgc gccaaacaga ttgctatggg cgtagacggt gccaacgaag tgtataacga
     9361 gattcgtcag ggccagccga ttggtctggg cgaagcgtct aacgatacgt ggatcaccac
     9421 caaagtgcgt tcgcagctct taaccagcga cctggtgaaa tcgtccaacg tgaaagtgac
     9481 caccgaaaac ggtgaagtgt tcctgatggg gctggtgact gaacgtgaag cgaaagcggc
     9541 ggcagatatt gccagccggg tgagcggcgt aaagcgggta accacagcgt ttacgtttat
     9601 taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa
     9661 cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa
     9721 tcagaacaac agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt
     9781 cagccataag gctttcgctg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac
     9841 cgccgggagc gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat
     9901 cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgctaccgc
     9961 catcgccatc acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca
    10021 gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa
    10081 aagcgtccat agcgccctgc cccagcggct aaaaaaaccg ccctgtgctt ccggtatgtc
    10141 aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc
    10201 aatcagcaac accatcacca gcccggccac cagacgaatc gccgcaaaac cccagccgag
    10261 gacaaacccc ataaacacca gcgtcgccgg gtttaacacc ggattgccca tccagaatgc
    10321 cagcgcaccg cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca
    10381 gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg
    10441 cgattgccca agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc
    10501 gaggatcacc cccagcactg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc
    10561 caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg
    10621 ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc
    10681 ggcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg
    10741 agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg
    10801 aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga
    10861 agagcttaaa atcgtcacac cttcatgccc gggccgcatc gcctctgcca gcatgacccg
    10921 cgccacgtct ctcgcatcaa tggatttcca gttaccgggt aataaacgga acagcggcgc
    10981 aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc
    11041 aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg
    11101 gttatagaaa aacggcgagt gggcattggc acccatagca ctgaccacca gcatatgctg
    11161 cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat
    11221 aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc
    11281 gatgggatcg gtgacctgcg ccagcgcgtc actcagttgc ggatcatggg gattaaacac
    11341 cccgggcata tcgcccaacg gacgtcgcgt cggcgcagca atggcgttaa ctttcggttc
    11401 gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac
    11461 ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca
    11521 ggcttaatag accatgaggt aattatcccc gattgtggga aattcgcctc atccaatgca
    11581 acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgagga
    11641 ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat
    11701 tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga
    11761 taaatccatc gatgaagtga cgcctgcgga gtttgatgcc ctgctgctac cgggcggcca
    11821 ttcaccggat tatctgcgtg gggacaaccg ttttgtcacc tttacccgtg attttgtgaa
    11881 tagtggcaaa ccggtgtttg ccatctgtca cggcccgcag ttgctgatca gcgccgatgt
    11941 gattcggggg cgcaagctga ccgcagttaa accgatcatt attgatgtta aaaatgcggg
    12001 cgcggaattt tacgatcagg aagttgtggt tgataaagat cagctggtta ccagccggac
    12061 accggacgat ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagtcgc
    12121 gtagccaaat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga
    12181 gccggatttc ccataccggg gccgacagca ttctggcaac cggaaagcga cggttgtacg
    12241 ccttgcgtgc gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca
    12301 caccgcgaat taacgctact gttttcggct gaccgtttac cgttccggca actgccgcct
    12361 gcggcccact catctgcgca tgacgtgttt tttcttccgt caaaatgtag aaggcgactt
    12421 tctgtgcatc aaaaagataa aaggcattgg cgcaccagag ttccccttcc tgctgcacac
    12481 accaggtgac aacatgttgt tttgccagcc aacgactgat ggcggtgagt gtttccattg
    12541 ctgttctctt ttatactgtt ggcctgacct taacatgtga cgcagacgat gacaccctgg
    12601 tatctttact tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc
    12661 gaacgccgct atcagcaaca tcaaagcggc aaaggggcga aagcactgcg cgggaaagga
    12721 gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt acgggcagaa
    12781 tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga aggcgcaggg
    12841 tttgcagagc tgttgagcag cctgcaaacc ccaaagatta aaagcgattg aaatgctcgt
    12901 gatactcaac caggccggta acgccattca gcgcgtcatc cgctaaacga tgtacctgga
    12961 aggcgctttc agtgcccggc cagcggcaac gcagatcgtg atgcgccgcc agttcaaagc
    13021 cgaaacgact gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat
    13081 taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt
    13141 catcaaccgc caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg
    13201 gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg
    13261 tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg
    13321 agcgacgcag cagggcatca atacccggcg catcaatggg aatttctact cgaattagca
    13381 tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa
    13441 atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc
    13501 gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg
    13561 ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc
    13621 gtcggcacta aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac
    13681 caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc
    13741 aagaaactcc agctcaccat catccagcgc ctgacggaat tgccagctta agacctgctc
    13801 aagaacctgg cgttttagcg caaatggcgt cagttttatc ggtacactca acagagatgg
    13861 ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa
    13921 caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat
    13981 atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc
    14041 aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg
    14101 aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga
    14161 aaacggcgca gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc
    14221 cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg
    14281 ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca
    14341 gcgcgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc
    14401 catgctggaa tacgccgccg agcgttatcc gcatattgag cgccacgtat cggtgcaggc
    14461 ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgcccgcgt
    14521 ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacag ctggcacggg tcacacctgt
    14581 accactggaa gtgtttgctt tcggcagcct gtgcattatg tcggaaggcc gttgctatct
    14641 gtcgtcgtat ctgacgggtg agtcgcctaa caccgtgggc gcgtgttctc cggcccgttt
    14701 cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga
    14761 ccgttatcag gacggcgaaa acgcaggtta tccgacgctg tgtaaagggc gttatctggt
    14821 ggacggcgag cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct
    14881 gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc
    14941 ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga
    15001 tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg
    15061 cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaagagc aatgaaatat
    15121 tccttagggc cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag
    15181 gccgccacca gcagcgccga cgtgatttat cttggtgaag cggtatgcag caagcgccgg
    15241 gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag
    15301 attgtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga actgaaacgc
    15361 tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc
    15421 gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgacg
    15481 ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc
    15541 gactggctgg tgaatctgct taatcagtgc gatgagctgg gcattcgcaa ccagtttgaa
    15601 gtggaagtcc tgagctacgg tcatctaccg ctggcctact ccgcccgctg ctttaccgcg
    15661 cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg
    15721 cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg
    15781 agcggctacg tttacaacct cggtaacgag ctggcatcca tgcaggggct ggttgatgtg
    15841 gtccgcctgt caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat
    15901 gaaaatggcg ctgcgccact accgttgacg gcaaacagcg actgtaacgg ctactggcgg
    15961 cggctggccg ggctggagct gcaagcctaa gtaaatatgc tcgtcatact tcaagttgca
    16021 tgtgctgcat ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggattca
    16081 ctctcttgtc gccttcctgc aactcgaatt atttagagca cagctcactt tgttaacaac
    16141 tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac
    16201 tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag gttcttcagc
    16261 gcgggaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca
    16321 tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg ccacgtcggt
    16381 attgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat
    16441 gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctca atacactcta
    16501 tccggggcga atcgatttgg ggctgggtcg tgcgccaggt agtgaccaac ggacaatgat
    16561 ggcgctgcgt cgccatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct
    16621 ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg
    16681 cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac tggcggcgca
    16741 gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct
    16801 gcatctttat cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat acgcgatggt
    16861 gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct gagtttctgt ttacctcaat
    16921 gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg caactgccgc caccgattca
    16981 aaatatggat cagttctggt cgccgtccga acagtatggt gtgcagcagg cgctgagtat
    17041 gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc tgcgcgaaac
    17101 cgacgccgat gagattatgg tcaacgggca gattttcgac caccaggcgc ggctgcattc
    17161 gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga
    17221 agccttatcc gagctggcaa cgctgtcct
//
LOCUS       DAFAVF010000087        16988 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFAVF010000087 DAFAVF010000000
VERSION     DAFAVF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.087"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..534
                     /gene="wcaD"
                     /locus_tag="LGK42_002865"
     CDS             <1..534
                     /gene="wcaD"
                     /locus_tag="LGK42_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBK2659094.1"
                     /translation="WAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLG
                     TEGSSSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYL
                     LIIYFSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYA
                     FLIVCPFILRKALNITR"
     gene            545..1291
                     /gene="wcaE"
                     /locus_tag="LGK42_002866"
     CDS             545..1291
                     /gene="wcaE"
                     /locus_tag="LGK42_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBK2659095.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            1307..1855
                     /gene="wcaF"
                     /locus_tag="LGK42_002867"
     CDS             1307..1855
                     /gene="wcaF"
                     /locus_tag="LGK42_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBK2659096.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            1881..3002
                     /gene="gmd"
                     /locus_tag="LGK42_002868"
     CDS             1881..3002
                     /gene="gmd"
                     /locus_tag="LGK42_002868"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBK2659097.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            3005..3970
                     /gene="fcl"
                     /locus_tag="LGK42_002869"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             3005..3970
                     /gene="fcl"
                     /locus_tag="LGK42_002869"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBK2659098.1"
                     /translation="MSKQRVFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            3973..4452
                     /gene="gmm"
                     /locus_tag="LGK42_002870"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             3973..4452
                     /gene="gmm"
                     /locus_tag="LGK42_002870"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBK2659099.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            4449..5672
                     /gene="wcaI"
                     /locus_tag="LGK42_002871"
     CDS             4449..5672
                     /gene="wcaI"
                     /locus_tag="LGK42_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBK2659100.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLVAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHIADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            5675..7111
                     /gene="cpsB"
                     /locus_tag="LGK42_002872"
     CDS             5675..7111
                     /gene="cpsB"
                     /locus_tag="LGK42_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBK2659101.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLKGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAIPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            7304..8674
                     /gene="cpsG"
                     /locus_tag="LGK42_002873"
                     /gene_synonym="manB"
     CDS             7304..8674
                     /gene="cpsG"
                     /locus_tag="LGK42_002873"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBK2659102.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANNFPPVDETKRGRYQQINLRDAYIDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKVLGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LALLNE"
     gene            8729..10123
                     /gene="wcaJ"
                     /locus_tag="LGK42_002874"
     CDS             8729..10123
                     /gene="wcaJ"
                     /locus_tag="LGK42_002874"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBK2659103.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            10125..11603
                     /gene="wzxC"
                     /locus_tag="LGK42_002875"
     CDS             10125..11603
                     /gene="wzxC"
                     /locus_tag="LGK42_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBK2659104.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGYSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            11879..13159
                     /gene="wcaK"
                     /locus_tag="LGK42_002876"
     CDS             11879..13159
                     /gene="wcaK"
                     /locus_tag="LGK42_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBK2659105.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSCERQTGMQMVQSVLERIGEAK"
     gene            13156..14376
                     /gene="wcaL"
                     /locus_tag="LGK42_002877"
     CDS             13156..14376
                     /gene="wcaL"
                     /locus_tag="LGK42_002877"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBK2659106.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            14387..15781
                     /gene="wcaM"
                     /locus_tag="LGK42_002878"
     CDS             14387..15781
                     /gene="wcaM"
                     /locus_tag="LGK42_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBK2659107.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPDQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQIFAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFILPERRE"
     gene            15939..16934
                     /locus_tag="LGK42_002879"
     CDS             15939..16934
                     /locus_tag="LGK42_002879"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HBK2659108.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHNPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
BASE COUNT         4122 a         4225 c         4651 g         3990 t
ORIGIN      
        1 tgggcatttc aatatctgaa taaagaggcg attaagaaaa agttaccgtt agcattgatt
       61 tctctggctg tattcctggt tggtgtggta atcgcgtttc cttatatttc cacccgtctg
      121 ggcgatttag gtacggaagg atcgtcatct tattatcgta ttgtcggtcc gctggtgatg
      181 gtcggttatt ctttgaccca tattgacggt gtagtcagat ttggctcact ttatgaatat
      241 gtcgcatcat tcggaatatt taacggtgcg gatgtcggaa aaaccataga caatggtttg
      301 tatctgctga ttatttattt ttcctggttc gcggtgtttt tatcactgtg gtacatgggg
      361 aaagtgataa aaatgatgat caacgctttt ggtgataacc gcaattttcg cgtgcaatta
      421 tatcttttta ctccggtatc gctgtttttt accggttcga tatttagccc ggaatatgca
      481 tttttaatcg tctgtccgtt tattttgcga aaagcgttaa atattacgag gtaagaataa
      541 gaacatgttg cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga tagtcaaaac
      601 acatgcctcg ctggcgcatc tggcgcaggt ggaagatatc agcttcgaat ggattgttgt
      661 cgatggcggt tccaacgacg gcacccgtga gtatctggaa aatctcaatg gtatctttaa
      721 cctacgcttt gtcagcgagc cagataacgg tatctacgac gccatgaaca aaggcattgc
      781 gatggcacaa ggcaagttcg cgttgtttct caattcgggc gatatttttc atcagaatgc
      841 cgcaaatttt gtccgtaagt taaaaatgca aaaagataac gtgatgatca ccggcgatgc
      901 gctgctggat tttggcgacg ggcataaaat taaacgtagc gccaaaccgg gctggtatat
      961 ttatcacagc ctgcccgcca gtcatcaggc gatatttttc cctgtatccg gcttgaaaaa
     1021 atggcgttat gacctggaat ataaagtttc ttccgactac gcgctggcag ccaaaatgta
     1081 taaagccggt tatgcattta aaaaactcaa tggcctggtg tctgaatttt ccatgggtgg
     1141 ggtatctacc accaataata tggaattgtg tgctgacgcg aaaaaagtcc aacgacaaat
     1201 attacatgtg cctggctttt gggctgaatt atcctggcat ttacgccaac gtactacctc
     1261 aaagacgaaa gccttatata acaaagtctg aatataagga aaaccaatgc aagatttaag
     1321 cggtttctcg gtgccgaaag ggttccgggg cggcaacgct attaaagtgc aattatggtg
     1381 ggcagtacag gcaacaatat ttgcctggtc gccacaagta ttgtatcgct ggcgggcttt
     1441 tttattacgt ttattcggag caaaaatagg aaaaaacgta gttattcgtc cgtcagtaaa
     1501 aattacctat ccgtggaaat taaccttagg tgattacgcg tgggtcggcg atgacgtcaa
     1561 tttatatacc ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc aaaaaagtta
     1621 tttatgcacc ggtagccacg accatgcaag tcaacatttc accattaacg ccacgcctat
     1681 tgtgattggc gagaaatgct ggctggcaac cgatgtcttt gttgccccag gcgtcacaat
     1741 cggcgacggc accgtcgtgg gtgcacgaag cagtgttttt aaatcgcttc cggcaaatgt
     1801 ggtttgccgg gggaatcccg cagtggtgat acgcgaacgc gttgaaactg aataaattca
     1861 aaaatacaga ggaataatac atgtcaaaag tcgctctcat caccggtgta accggacaag
     1921 acggttctta cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc
     1981 gtcgcgcatc gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct
     2041 gcaacccgaa attccatctg cattatggcg acctgagtga tacctctaac ctgacgcgca
     2101 ttttgcgtga agtacagccg gatgaagtgt acaacctggg cgcaatgagc cacgttgcgg
     2161 tctcttttga gtcaccagaa tataccgctg acgtcgacgc gatgggtacg ctgcgcctgc
     2221 tggaggcgat ccgcttcctc ggtctggaaa agaaaactcg tttctatcag gcttccacct
     2281 ctgaactgta tggtctggtg caggaaattc cgcagaaaga gaccacgccg ttctacccgc
     2341 gatctccgta tgcggtcgcc aaactgtacg cctactggat caccgttaac taccgtgaat
     2401 cctacggcat gtacgcctgt aacggaattc tcttcaacca tgaatccccg cgccgcggcg
     2461 aaaccttcgt tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt
     2521 cgtgcctgta cctcggcaat atggattccc tgcgtgactg gggccacgcc aaagactacg
     2581 taaaaatgca gtggatgatg ctgcaacagg aacagccgga agatttcgtt atcgcgaccg
     2641 gcgttcagta ctccgtgcgt cagttcgtgg aaatggcggc agcacagctg ggcatcaaac
     2701 tgcgctttga aggcacgggc gttgaagaga agggcattgt ggtttccgtc accgggcatg
     2761 acgcgccggg cgttaaaccg ggtgatgtga ttatcgctgt tgacccgcgt tacttccgtc
     2821 cggctgaagt tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa ctgggctgga
     2881 aaccggaaat caccctcaga gagatggtgt ctgaaatggt ggctaatgac ctcgaagcgg
     2941 cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat
     3001 aagcatgagt aaacaacgag tttttattgc tggtcatcgc gggatggtcg gttccgccat
     3061 caggcggcag ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc gcgacgagct
     3121 gaacctgctg gacagccgcg ccgtgcatga tttctttgcc agcgaacgta ttgaccaggt
     3181 ctatctggcg gcggcgaaag tgggcggcat tgttgccaac aacacctatc cggcggattt
     3241 catctaccag aacatgatga ttgagagcaa catcattcac gccgcgcatc agaacgacgt
     3301 gaacaaactg ctgtttctcg gatcgtcctg catctacccg aaactggcaa aacagccgat
     3361 ggcagaaagc gagttgttgc agggcacgct ggagccgact aacgagcctt atgctattgc
     3421 caaaatcgcc gggatcaaac tgtgcgaatc atacaaccgc cagtacggac gcgattaccg
     3481 ctcagtcatg ccgaccaacc tgtacgggcc acacgacaac ttccacccga gtaattcgca
     3541 tgtgatccca gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt
     3601 ggtggtatgg ggcagcggta caccgatgcg cgaatttctg cacgtcgatg atatggcggc
     3661 ggcgagcatt catgtcatgg agctggcgca tgaagtctgg ctggagaaca cccagccgat
     3721 gttgtcgcac attaacgtcg gcacgggcgt tgactgcact atccgcgagc tggcgcaaac
     3781 catcgccaaa gtggtgggtt acaaaggccg ggtggttttt gatgccagca aaccggatgg
     3841 cacgccgcgc aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat
     3901 ctcactggaa gcggggcttg ccagcactta ccagtggttc cttgagaatc aagaccgctt
     3961 tcgggggtaa tgatgttttt acgtcaggaa gactttgcca cggtagtgcg ctccactccg
     4021 cttgtctctc tcgactttat tgtcgagaac agtcgcggcg agtttctgct tggcaaaaga
     4081 accaaccgcc cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa
     4141 acgctggaag ccgcatttga gcggctgacg atggcggaac tggggctgcg tttgccgata
     4201 acagcaggcc agttttacgg tgtctggcag cacttttatg acgataactt ctccggcacg
     4261 gatttcacca ctcactatgt ggtgctcggt tttcgcttca gagtatcgga agaagagctg
     4321 ttactgccgg atgagcagca tgacgattac cgctggctga cgccggatgc actgctcgcc
     4381 agtgacaatg ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta
     4441 cccggattat gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg
     4501 gcaaatacac tggcgagatg gtggaatggc tggtagcaca aggtcatgag gtgcgggtca
     4561 ttaccgcacc gccttactac ccgcagtggc aggtgggcga gaactattcc gcctggcgct
     4621 acaaacgaga agagggggcc gccacggtgt ggcgctgccc gctgtacgtg ccaaaacagc
     4681 cgagcaccct gaaacgcctg ttacatctcg gcagttttgc cgtcagcagt ttctttcctc
     4741 tgatggcgca acgtcgctgg aagccggatc gcattatcgg cgtggtgcca acgctgtttt
     4801 gcacgccggg aatgcgcctg ctggcgaaac tctctggtgc gcgtaccgtg ctgcatattc
     4861 aggattacga agtggacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca
     4921 aagttgcaca gctggcaacg gcgttcgaac gtagcggact gcataacgtc gataacgtct
     4981 ccacgatttc gcgctcgatg atgaataaag ccatcgaaaa aggcgtggcg gcggaaaacg
     5041 tcatcttctt ccccaactgg tcggaaattg cccgttttca gcatattgca gatgccgatg
     5101 ttgatgccct tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttactccg
     5161 gcaatattgg tgaaaagcag gggctggaaa acgttattga agctgccgat ctcctgcgcg
     5221 atgaaccgct gatttttgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa
     5281 tggcgcaaca gcgtggtctg cgcaacatgc aattttttcc gctgcaatcg tatgacgctt
     5341 tacccgcact gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag
     5401 atgccgtatt gccgtcgaaa ctgaccaata ttctggctgt aggcggtaac gcggtgatta
     5461 ctgcagaagc ccacacagaa ctgggacagc tttgcgaaac ctttccgggc attgcggttt
     5521 gcgttgaacc ggaatcggtc gaggcgctgg tggcggggat tcgtcaggcg ctcctgctgc
     5581 ccaaacacaa cacggtggca cgtgaatatg ccgaacgcac gctcgataaa gagaacgtgt
     5641 tacgtcaatt tataaatgat attcggggat aattatggcg cagtcgaaac tctatccagt
     5701 tgtgatggca ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttaccccaa
     5761 gcagttttta tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa
     5821 aggcgtagag tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct ttattgtcgc
     5881 ggaacagctg cgccaactga acaaactcac cgagaacatt attctcgaac cggcagggcg
     5941 taacactgca ccagccattg cgctggcggc gctggcggca acacgtcata gtccggagag
     6001 cgacccgtta atgctggtgc tggcggcgga tcatgtgatt gccgatgaag acgcgttccg
     6061 tgccgccgtg cgtaatgcca taccgtatgc cgaagcgggc aagctggtga cctttggcat
     6121 tgtgccggat ctaccagaaa ccggttatgg ctatattcgt cgcggtgaag tatctgcggg
     6181 cgagcaggat acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga atctggaaac
     6241 cgcccaggcc tatgtggcaa gcggcgaata ttactggaac agtggcatgt tcctgttccg
     6301 tgccggacgc tatctcgaag aactgaaaaa atatcgtccg gatattctcg atgcctgcga
     6361 aaaagcgatg agcgccgtcg atccggatct cgattttatt cgcgtggatg aagaagcgtt
     6421 tctcgcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgccgt
     6481 tgtggtgccg atggatgcgg gctggagcga tgtcggttcc tggtcctctt tatgggagat
     6541 cagcgcccac accgcggaag gcaacgtttg ccacggcgat gtgattaatc acaaaactga
     6601 aaacagctat gtgtacgccg aatctggcct ggtcaccacc gtcggggtga aagatctggt
     6661 ggtggtgcag accaaagatg cggtgctgat tgccgaccgt aacgcggtgc aggatgtgaa
     6721 aaaagtggtc gagcagatca aagccgatgg tcgccatgag catcgggtac atcgcgaggt
     6781 ctatcgcccg tggggcaaat atgactctat tgacgcgggc gaccgctatc aggtgaaacg
     6841 catcaccgtg aaaccgggcg aaggcttgtc agtacagatg catcatcacc gcgcggaaca
     6901 ctgggtagtg gtcgcgggaa cggcaaaagt caccattgac ggtgatatca aactgcttgg
     6961 tgaaaacgag tccatttata ttccgctggg ggcgacgcac tgcctggaaa acccggggaa
     7021 aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt
     7081 gcgcttcgcg gatcgctacg gacgggtgta aacgtcgcat caggcgctgt ttgtcggatg
     7141 cggcgtgaac gccttatccg acctacggtt cggttttgta ggcctgataa gacgcggcag
     7201 cgtcgcatca ggcaacgaat gcgaaaccgc ggtgtaaata acgacaaaaa caaaattggc
     7261 cgcttcggtc agggccaact attgcctgaa aaagggtaac gatatgaaaa aattaacctg
     7321 ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag atatcgcctg
     7381 gcgcattggt cgcgcctatg gcgaatttct caaaccgaaa accattgtgt taggcggtga
     7441 tgtccgcctc accagcgaaa ccttaaaact ggcgctggcg aaaggtttgc aagatgcggg
     7501 cgtcgacgtg ctggatattg gtatgtccgg caccgaagag atctatttcg ccacgttcca
     7561 tctcggcgtg gatggcggca ttgaagttac cgccagccat aatccgatgg attataacgg
     7621 catgaagctg gtgcgcgaag gggctcgccc aatcagcggt gataccggac tgcgcgacgt
     7681 ccagcgtctg gctgaagcca acaactttcc tcccgtcgat gaaaccaaac gcggtcgcta
     7741 tcagcaaatc aacctgcgtg acgcttacat tgatcacctg ttcggttata tcaatgtcaa
     7801 aaatctcacg ccgctcaagc tggtgattaa ctccgggaat ggcgcagcgg gtccggtagt
     7861 tgacgccatt gaagcccgct ttaaagtcct cggcgcaccc gtggaattga tcaaagtgca
     7921 caacacgccg gacggtaatt tccccaacgg tattcctaac ccgctactgc cagaatgccg
     7981 cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat atgggcattg cctttgatgg
     8041 cgattttgac cgctgcttcc tgtttgacga aaaagggcag tttattgagg gctactacat
     8101 tgtcggcctg ttggcagaag cattcctcga aaaaaatccc ggcgcgaaga tcatccacga
     8161 tccacgtctc tcctggaaca ctattgatgt ggtgactgct gcaggcggca cgccggtaat
     8221 gtcgaaaacc ggacacgcct ttattaaaga acggatgcgc aaggaagacg ccatctacgg
     8281 tggtgaaatg agcgcccacc attacttccg tgatttcgct tactgcgaca gcggcatgat
     8341 cccatggctg ctggtcgccg aactggtgtg cctgaaagga aaaacgctgg gcgaactggt
     8401 acgcgaccgg atggcggcgt ttccggcaag cggtgagatc aacagcaaac tggcgcaacc
     8461 cgttgaggcg attaaccgcg tggaacagca ctttagccgt gaggcgctgg cggtggatcg
     8521 caccgatggt atcagcatga cctttgccga ctggcgcttt aacctgcgct cctccaacac
     8581 cgaaccggtg gtgcgcctga atgtggaatc gcgcggtgat gtgccgctga tggaagcgcg
     8641 aacgcgaact ctgctggcgt tgctgaacga gtaatgtcgg atcttccctt accccattgc
     8701 gggtaagggg ctaataacag gaacaacgat gacaaatcta aaaaagcgcg agcgagcgaa
     8761 aaccaatgca tcgttaatct ctatggtgca acgcttttca gatatcacca tcatgtttgc
     8821 cggactatgg ctggtttgcg aagtgagcgg cctgtcattc ctctacatgc acctgttggt
     8881 ggcactgatt acgctggtgg tgttccagat gctgggcggc atcaccgatt tttatcgctc
     8941 atggcgcggt gttcgggcag cgacagaatt tgctctgttg ctacaaaact ggacgttaag
     9001 cgtgattttc agcgccggac tggtggcgtt caacaatgat ttcgacacgc aactgaaaat
     9061 ctggctggcg tggtatgggc tgaccagcat cggactggtg gtttgccgtt cgtgtattcg
     9121 tattggggcg ggctggctgc gtaatcatgg ctataacaag cgtatggtcg cggtggcggg
     9181 ggatttagcc gccggacaaa tgctgatgga gagcttccgt aatcagccgt ggttagggtt
     9241 tgaagtggtg ggggtttacc acgacccgaa accgggcggc gtttctaacg actgggcggg
     9301 caatctgcaa cagctggtcg aggatgcgaa agcgggtaaa attcataacg tctatatcgc
     9361 gatgcagatg tgcgacggcg cgcgagtgaa aaaactggtc catcaactgg cggataccac
     9421 ctgttcggtg ctgctgatcc ccgatgtctt taccttcaac attctccatt cacgtctcga
     9481 agagatgaac ggcgttccgg tggtgccgct ttacgacacg ccgctttccg gggttaaccg
     9541 cctgctcaaa cgtgcggaag acattgtgct ggcgacgctt attctgttgc tgatctcccc
     9601 ggtgctgtgc tgtattgcgc tggcggtgaa actcagttca ccagggccgg ttattttccg
     9661 ccagactcgc tatggcatgg atggcaagcc gatcaaagtg tggaagtttc gttccatgaa
     9721 agtgatggag aacgacaaag tggtgaccca ggcgacgcag aacgatccgc gcgtcaccaa
     9781 agtggggaac tttctgcgcc gcacctcgct ggatgaattg ccgcagttta tcaatgtgct
     9841 gaccgggggg atgtcgattg tcggtccacg tccacacgcg gtggcgcata acgaacagta
     9901 tcgccagctc attgaaggct acatgctgcg ccataaggtg aaaccgggca ttaccggctg
     9961 ggcgcagatt aatggctggc gtggcgaaac cgacacgctg gagaaaatgg aaaaacgcgt
    10021 cgagttcgac cttgagtaca tccgcgaatg gagcgtctgg ttcgatatca aaatcgtttt
    10081 cctgacggta ttcaagggct tcgttaacaa agcggcatat tgatatgagc ttacgtgaaa
    10141 agaccatcag cggcgcgaag tggtcggcga ttgccacggt gatcatcatc ggcctcgggc
    10201 tggtgcagat gaccgtgctg gcgcggatta tcgacaacca ccagttcggt ttgcttaccg
    10261 tgtcgctggt gatcatcgcg ctggcagata cgctttctga cttcggtatc gctaactcga
    10321 ttattcagcg aaaagaaatc agccacctgg aactcaccac gctgtactgg ctgaacgtcg
    10381 ggctggggat cgtggtgtgt gtggcggtgt ttttgttgag tgatgccatt ggtgacgtgc
    10441 tgaataaccc ggacctggca ccgttgatta aaacattgtc gctggcgttt gtggtgatcc
    10501 cccacgggca acagttccgc gcgttgatgc aaaaggaact ggagttcaac aaaatcggca
    10561 tgatcgaaac cagcgcggtg ctggcgggct tcacttttac ggtggttagc gcccatttct
    10621 ggccgctggc gatgaccgcg atcctcggtt atctggtcaa tagtgcggta agaacgctgc
    10681 tgtttggcta ctttggtcga aaaatttatc gccccggtct gcatttctcg ctggcgtcag
    10741 ttgcgccgaa cttacgcttt ggtgcctggc tgacggcgga cagcatcatc aactatctca
    10801 acaccaacct ttcaacgctg gtgctggcgc gtattcttgg cgcgggcgtg gcagggggat
    10861 acaacctggc gtacaacgtg gccgtcgtac cgccgatgaa gctgaaccca atcatcaccc
    10921 gcgtgttatt tccggcgttt gccaaaattc aggacgacac cgaaaagttg cgcgtgaact
    10981 tctacaagct gctgtcggtg gtggggatta tcaactttcc ggcgctgctc gggctgatgg
    11041 tggtgtcgaa taactttgta ccgctggtct ttggtgagaa gtggaacagc attattccgg
    11101 tgctgcaatt gctgtgtgtg gtggggctac tgcgctccgt gggtaacccg attggttcgc
    11161 tgctgatggc gaaagcgcgg gtcgatatca gctttaaatt caacgtattc aaaacatttc
    11221 tgtttattcc ggcgattgtt attggtgggc agatggcggg cgcaatcggc gtcacgctcg
    11281 gtttcctgct ggtgcagatt atcaacacca ttctgagtta cttcgtgatg attaaaccgg
    11341 ttcttggtta cagttatcgc cagtacatcc tgagtttgtg gctgccgttt tatctctcgt
    11401 tgccgacgct ggtggttagt tatgcgctgg gcattgtgct gaaagggcaa ctggcgctgg
    11461 ggatgctgct ggcggtgcaa atagccgcgg gcgtgctggc gtttgtggtg atgattgtgc
    11521 tgtcgcgcca tccgctggtg gtggaagtga agcgtcagtt ttgtcgcagc gaaaaaatga
    11581 aaatgctttt acgggcgggg tgagttgctg ttttcggttc cgacaacggt tgccggatgc
    11641 ggcgtaaaac gccttatcca tcctacggtt ctgtgctcgg gacctgtagg cctgataaga
    11701 cgcgtcagcg tcgcatcagg cactgaatgc cggatgcggc gtgaacgcct tatccggcct
    11761 acggttctgt gctcgggtct tgtaggcctg ataagacgcg tcagcgtcgc atcaggcaaa
    11821 aagcggcaac gaaaaaaaca aacgcagcct cacctggcat caaatttatg aggaaaaaat
    11881 gaaattactt attctgggca accacacttg cggcaatcgt ggcgacagcg ccatcctgcg
    11941 cggcttactt gatgccatca acattctcaa tccacacgcc gaagtggatg tgatgagccg
    12001 ctatccggtc agttcttcct ggctgctcaa ccgcccggta atgggcgatc cgctgttcct
    12061 gcaaatgaaa caacacaaca gcgcagcggg cgttgtcggg cgcgttaaaa aagtcctccg
    12121 tcgccgctac cagcatcagg tactgctctc gcgcgtcacc gacactggca agttgcgtaa
    12181 tatcgccatc gcccagggat tcactgactt cgtgcgcctg ctgtcaggtt acgacgccat
    12241 tattcaggtc ggcggatcgt tttttgtcga tctctacggc gtgccacagt ttgaacatgc
    12301 actttgcacg tttatggcga aaaagccgct gtttatgatt ggtcacagcg ttggcccgtt
    12361 ccaggatgag caatttaacc aactggcgaa ctatgtcttt ggtcactgtg acgcgctgat
    12421 cctgcgcgaa tcggtcagcc ttgatctgat gaaacgcagc aatatcacca ccgcaaaagt
    12481 ggaacatggc gtcgataccg cgtggctggt cgatcaccac acagaagact tcaccgccag
    12541 ctatgccgtt caacactggc tggacgttgc cgcacaacag aaaacggtgg ccattaccct
    12601 gcgcgaactg gcaccgttcg acaaacgtct cggcaccact caacaagcgt atgaaaaagc
    12661 ctttgccggg gtggtcaatc gcattctcga cgaaggctat caggtgattg cgctctccac
    12721 ctgtacgggc atcgacagct ataacaaaga cgaccgcatg gtggcgctca acctgcgcca
    12781 gcacatcagc gatcctgccc gttaccacgt ggtgatggat gaactcaacg atctggaaat
    12841 gggcaaaatt ctcggagcct gtgaactcac cgtcggtacg cgcctgcact ccgccattat
    12901 ctcgatgaat ttctccactc cggcaattgc catcaactac gaacataaat ccgccgggat
    12961 tatgcaacaa ctggggctgc cagagatggc gattgatatc cgtcatttac tggacggcag
    13021 cttgcaagcg atggttgcgg ataccttagg ccaacttccg gcgctgaacg cgcgacttaa
    13081 cgaagccgtc agttgcgaac gtcagacggg aatgcagatg gtgcagtctg tactggaacg
    13141 catcggggag gcgaaatgaa ggtcggcttc tttttactga aatttccgct gtcgtcggaa
    13201 actttcgtcc tcaatcaaat taccgcgttt attgatatgg gatttgaggt ggagattgtc
    13261 gcgctgcaaa aaggcgatac agagaacacc cacgcggcat ggacgaaata caaccttgcc
    13321 gccagaaccc gctggttaca ggacgaacca acgggcaaag tggcgaagct gcgccaccgc
    13381 gccagccaga ccttacgcgg catttatcgt aaaaatacct ggcaggcgct caacctcaaa
    13441 cgctatggtg ccgagtcgcg gaacctgatt ttgtctgcca tttgtggcca ggtcgcaaca
    13501 ccgtttcatg ccgatgtctt tatcgctcat tttggcccgg cgggggtaac cgcagcaaaa
    13561 ctacgcgaac tgggtgttat tcgcggcaaa attgccacta tcttccacgg tattgatatc
    13621 tccagtcggg acgttctcaa ccactacact cccgaatatc agcaactgtt ccgccgtggc
    13681 gacctgatgc taccgatcag cgatctctgg gccggaaggc tgcaaaaaat gggctgtccg
    13741 agggaaaaaa tcgccgtatc gcgcatgggc gtggacatga cgcgctttag cccgcgtccg
    13801 gtgaaagcgc ccgcaacgcc gctggaaatc atttccgtcg cacgcttaac cgaaaaaaaa
    13861 ggcctgcatg tggcgatcga agcctgccgt cagttgaaag agcagggcgt ggcatttcgc
    13921 tatcgcatcc tcggcattgg cccgtgggaa cgacgcctgc gcaccctcat cgaacaatat
    13981 caactggaag atgtggtgga gatgccgggc tttaaaccga gccatgaagt gaaagcgatg
    14041 ctcgacgacg cggatgtctt cctgttgcca tcggttacag gtgcggatgg tgatatggaa
    14101 ggcattccgg tggcgctaat ggaagcgatg gcggtcggca ttccggtggt ttctactctg
    14161 catagcggaa taccggaact ggttgaggcc gataaatccg gctggctggt gcctgagaac
    14221 gatgctcgcg cactggcgca acgcctggcg gcatttagcc aactggacac cgacgaactg
    14281 gctccggtcg tcaaacgtgc gcgcgaaaaa gtcgaacacg attttaacca gcaggtgatc
    14341 aatcgagaac tcgccagctt gctgcaggct ttatagagga gaacgcatgc catttaaaaa
    14401 actctcccga cgcacgttcc tgacggcaag ctcggcgctt gccttcctcc ataccccttt
    14461 cgctcgcgca cttcccgacc aacagagcgt taacattaac gactacaacc cacacgactg
    14521 gatcgcctca tttaaacaag ccttcagcga aggacaaaca gtcgtcgtgc ctgccggatt
    14581 ggtttgcgac aatatcaaca ccggcatctt cattcctcct ggcaaaacgt tacacatcct
    14641 tggaagcctg cgcggcaacg gcagagggcg atttgtctta caggacggca gccaggtgac
    14701 aggggaggag ggcggcagta tgcataacat caccctggat gtgcgcggct ctgactgcac
    14761 catcaaaggt ctggcgatga gcggctttgg cccggtaacg cagatttata tcggcggcaa
    14821 aaacaaacgg gtcatgcgca acctgaccat cgataacctg accgttagcc acgctaatta
    14881 cgccatctta cgccagggat ttcataacca gattatcggt gccaacatca ccaattgtaa
    14941 gttcagcgac ttacagggcg acgccattga atggaatgtg gcgattaacg acagtgatat
    15001 tttgatatct gaccatgtca tcgagcgtat caactgtacc aacggcaaaa tcaactgggg
    15061 catcggcata ggccttgcgg gaagtaccta cgataacaac tacccggaag accaggcagt
    15121 gaaaaacttt gtcgtggcga atatcacggg atcggattgt cggcagttga tccatgttga
    15181 aaatggtaaa cattttgtta ttcgaaatat caaagcccgc aatatcacac cggatttcag
    15241 taagaaagca ggtattgata acgccacagt cgctatttac ggttgtgaca atttcgtgat
    15301 tgataatatt gaaatgatta atagcgccgg gatgttaatc ggctatgggg taattaaagg
    15361 caaatatctc tcgataccgc aaaatttccg agtgaataat attcagctgg ataataccca
    15421 ccttgcttat aaattgcgcg gcatccaaat cttcgccggg aatgctgtct cctttgtggc
    15481 actgactaac attgagatga agcgtgcctc gttggagcta cacaataaac cgcaacacct
    15541 ttttatgcga aatatcaatg tgatgcagga atcctctgtt ggacccgcat tgagcatgaa
    15601 cttcgacatg cgcaaagacg ttcgcggcgt ctttatggcg aaaaaagaaa cactgctgtc
    15661 tcttgcaaat gttcatgcgg tgaatgaaag agggcaaagc tccgtcgata tcgacaggat
    15721 taatcaccat attgttaatg tggaaaagat taactttata ttgccggaac ggagagagta
    15781 gatttgcgac cattcataaa cttactggat ataatgttct aaaagattat aatccttacg
    15841 ctaactgtta aagctggggc tgggcagcat tgactactca gggaaggtac tcatctttat
    15901 cttgtgtttc ttacgtaaca accagactaa agtgatttat gaacgataac gttttgctca
    15961 taggagcttc cggattcgta ggaacccgac tacttgaaac ggcaattgct gactttaata
    16021 tcaagaactt ggacaaacag cagagccatt tttatccaga aattacacag attggcgatg
    16081 ttcgcgatca acaggcactc gaccaggcgt tagctggttt tgacactgtt gtactgctgg
    16141 cagcggaaca tcgcgatgat gtcagcccta cttctctcta ttatgatgtc aatgttcagg
    16201 gtacccgcaa cgtgctggcg gccatggaaa aaaatggcgt taaaaatatc atctttacca
    16261 gttccgttgc tgtttatggt ttgaataaac acaaccctga cgaaaaccat ccacacaacc
    16321 ctttcaacca ctacggcaaa agcaagtggc aggcagagga agtgctgcgt gaatggtata
    16381 acaaagcacc aacagaacgt tcattaacca tcatccgtcc taccgttatc ttcggtgaac
    16441 gcaaccgcgg taacgtctat aacttgctga aacagatcgc tggcggcaag tttatgatgg
    16501 tgggcgcagg gactaactat aagtccatgg cttatgttgg aaacattgtt gagtttatca
    16561 agtacaaact gaagaatgtt gccgcaggtt atgaggttta taattacgtt gataagccag
    16621 atctgaacat gaaccagttg gttgctgaag ttgaacaaag tctgaacaaa aagatccctt
    16681 ctatgcactt gccttaccca ctaggaatgt tgggtggata ttgctttgat atcctgagca
    16741 aaattacggg caaaaaatac gctgtcagct ctgtgcgcgt gaaaaaattc tgcgcaacaa
    16801 cacagtttga cgcaacgaaa gtgcattctt caggttttgt ggcaccgtat acgctgtcgc
    16861 aaggtctgga tcgaactctg cagtatgaat tcgttcatgc caaaaaagac gacataacgt
    16921 ttgtttctga gtaatgcaga cgagtcagag aaaaaaggac caggttggac tacgattatt
    16981 cggaaaga
//
LOCUS       DAFAVF010000088        16673 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFAVF010000088 DAFAVF010000000
VERSION     DAFAVF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.088"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="LGK42_002880"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="LGK42_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBK2659109.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="LGK42_002881"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="LGK42_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBK2659110.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="LGK42_002882"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="LGK42_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBK2659111.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="LGK42_002883"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="LGK42_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBK2659112.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="LGK42_002884"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="LGK42_002884"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK2659113.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="LGK42_002885"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="LGK42_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK2659114.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="LGK42_002886"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="LGK42_002886"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK2659115.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="LGK42_002887"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="LGK42_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK2659116.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="LGK42_002888"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="LGK42_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK2659117.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVIEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="LGK42_002889"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="LGK42_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK2659118.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="LGK42_002890"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="LGK42_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK2659119.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="LGK42_002891"
     tRNA            complement(13705..13781)
                     /locus_tag="LGK42_002891"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="LGK42_002892"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="LGK42_002892"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBK2659120.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..>16673)
                     /locus_tag="LGK42_002893"
     CDS             complement(15480..>16673)
                     /locus_tag="LGK42_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBK2659121.1"
                     /translation="ILLLIVISGSLFSACQFAYKDAKNKKAFSPYILASRFATYTPFF
                     NLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDTYVLIVGESVRVDNMSLYGYTRS
                     TTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTADSVLSHDIHNYPDNIINMANQAG
                     FQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFDELLLPHLSQALQQNTQQKKLIV
                     LHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIHYTDSLLGQVFELLKDRRASVMY
                     FADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWYSPVLGDGVDRTTENNIFSTAYN
                     NYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDANHDVFDYVMLRKEFTEDKQGNPT
                     SEGQG"
BASE COUNT         3985 a         4545 c         4039 g         4104 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacaatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccactgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgtgcatcg cccagtaact
     1681 gcatgttcat cggcttgttg agaatgcgag tacgcgtaaa gtgttgcagc acttcacccg
     1741 gcataccttt cggcagttcg atggtggagt gagaagcaaa cagcttgatg ttaccaatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcaccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaca gctgcatatc gccaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcaccacgcg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggtacgatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatccagct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgaagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtacagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tatccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcagtg cagagatacc
     5161 gtcgcgggaa cctgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg attggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagaa gaaccgttgg attcggtgat ttcagacact acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcggta cccagcgttg cagtaaccag tgcctgcgtt tcaccgcggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc ggtttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcgataacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tacctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttcg gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ctgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggattacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaaca tggcaaattc
    13801 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcat
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctccccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcacgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccaattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtag cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaggct ccggggatgg cactgctaca tatcgcttat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca tgcacatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcagaggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat taaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tat
//
LOCUS       DAFAVF010000089        16605 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFAVF010000089 DAFAVF010000000
VERSION     DAFAVF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.089"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            141..629
                     /locus_tag="LGK42_002894"
     CDS             141..629
                     /locus_tag="LGK42_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBK2659122.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(749..1141)
                     /gene="flhe"
                     /locus_tag="LGK42_002895"
     CDS             complement(749..1141)
                     /gene="flhe"
                     /locus_tag="LGK42_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBK2659123.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(1141..3219)
                     /gene="flhA"
                     /locus_tag="LGK42_002896"
     CDS             complement(1141..3219)
                     /gene="flhA"
                     /locus_tag="LGK42_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBK2659124.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(3212..4360)
                     /gene="flhB"
                     /locus_tag="LGK42_002897"
     CDS             complement(3212..4360)
                     /gene="flhB"
                     /locus_tag="LGK42_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBK2659125.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(4562..5206)
                     /gene="cheZ"
                     /locus_tag="LGK42_002898"
     CDS             complement(4562..5206)
                     /gene="cheZ"
                     /locus_tag="LGK42_002898"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBK2659126.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(5217..5606)
                     /gene="cheY"
                     /locus_tag="LGK42_002899"
     CDS             complement(5217..5606)
                     /gene="cheY"
                     /locus_tag="LGK42_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBK2659127.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(5621..6670)
                     /gene="cheB"
                     /locus_tag="LGK42_002900"
     CDS             complement(5621..6670)
                     /gene="cheB"
                     /locus_tag="LGK42_002900"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBK2659128.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(6673..7533)
                     /gene="cheR"
                     /locus_tag="LGK42_002901"
     CDS             complement(6673..7533)
                     /gene="cheR"
                     /locus_tag="LGK42_002901"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBK2659129.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(7552..9153)
                     /gene="tap"
                     /locus_tag="LGK42_002902"
     CDS             complement(7552..9153)
                     /gene="tap"
                     /locus_tag="LGK42_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBK2659130.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(9199..10860)
                     /gene="tar"
                     /locus_tag="LGK42_002903"
     CDS             complement(9199..10860)
                     /gene="tar"
                     /locus_tag="LGK42_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBK2659131.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(11005..11508)
                     /gene="cheW"
                     /locus_tag="LGK42_002904"
     CDS             complement(11005..11508)
                     /gene="cheW"
                     /locus_tag="LGK42_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBK2659132.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(11529..13493)
                     /gene="cheA"
                     /locus_tag="LGK42_002905"
     CDS             complement(11529..13493)
                     /gene="cheA"
                     /locus_tag="LGK42_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBK2659133.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(13498..14424)
                     /gene="motB"
                     /locus_tag="LGK42_002906"
     CDS             complement(13498..14424)
                     /gene="motB"
                     /locus_tag="LGK42_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBK2659134.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(14421..15308)
                     /gene="motA"
                     /locus_tag="LGK42_002907"
     CDS             complement(14421..15308)
                     /gene="motA"
                     /locus_tag="LGK42_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBK2659135.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(15435..16013)
                     /gene="flhC"
                     /locus_tag="LGK42_002908"
     CDS             complement(15435..16013)
                     /gene="flhC"
                     /locus_tag="LGK42_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBK2659136.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(16016..16366)
                     /gene="flhD"
                     /locus_tag="LGK42_002909"
     CDS             complement(16016..16366)
                     /gene="flhD"
                     /locus_tag="LGK42_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBK2659137.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3923 a         4643 c         4010 g         4029 t
ORIGIN      
        1 cctgatcagg cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt
       61 tctgactgtt ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt
      121 tgaatttaag gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt
      181 gccaggctta cgcagaagat acggttatag taaacgacca tgacatttca gccatcaaag
      241 attgttggca aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc
      301 gacaagaata caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt
      361 atatagaaca agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc
      421 ttaaaaaatc acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt
      481 caaatgagga cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta
      541 tgatcataca gaccaatacg cgaactcgcc agttgcagct ataccataac tctgaagatt
      601 tttatccaag ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag
      661 ctattcctga taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat
      721 actcgcgagc gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt
      781 gtacctttag cggtggaatt aaccgcccac cgcctggcac ttcccagata aatcgcaacg
      841 gttctgctgc cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga
      901 cacaacgaga ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct
      961 gataacgcca cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg
     1021 cagacgacat cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca
     1081 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca
     1141 ttatttgccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt
     1201 cgacaggacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag
     1261 cgcgtggttc accaacaata ctggcggcgc acccagcatc tcctgacggg atagcgcttc
     1321 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag
     1381 cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
     1441 gccaggaaac cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa
     1501 ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
     1561 atcgcgaatc ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag
     1621 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
     1681 caacagctgt tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
     1741 gttaagatgc gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc
     1801 ctgttctttt agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt
     1861 cgcctcacca ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg
     1921 ataagcatca ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg
     1981 cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa
     2041 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat
     2101 cgggatcagt cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca
     2161 cgtcgcttcg acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc
     2221 tttttgttcg cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa
     2281 aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
     2341 caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
     2401 ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ctggaatttg
     2461 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct
     2521 catgccatgt tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag
     2581 gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa
     2641 atcggcttcc tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc
     2701 agcgttaagg tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg
     2761 cgcacccact tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac
     2821 gagaatgaca aacaccacga taccgatagc gaaattgcca ccaacgagga agtgaccgaa
     2881 tgcttcgacc accttcccag ccgccgccgc gccggtatgc ccttccatca aaatgatacg
     2941 ggttgaagcc acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa
     3001 cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa
     3061 ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat
     3121 cgacaagatc aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag
     3181 gtttgcgggc aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa
     3241 aatccagggc ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca
     3301 gacgccagcg tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt
     3361 gacccgggat ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg
     3421 cttcaagcgt cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc
     3481 ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta
     3541 ccgaatagtg ggtcgggtta ttgacaatga catccgcttt cggcacatcg gccatcatcc
     3601 gacgccgtgc agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt
     3661 cgctttgttt gaactcatca cgaatatcct gccgtgacat acgcagcttt ttcaggtggc
     3721 tgaatatttg gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca
     3781 gtgcgcatag ccctaccaaa tccatcgcat tacccatggc ggtaatcgga gactcggcca
     3841 tcaagcgcat catctgcggc cagtgatgcc agagaaaaaa ccccgtcacg ctgccaacca
     3901 ggatggtttt caaaattgct ttaagcaact ccgcgccagt ctgagccgag aacatccgtt
     3961 taatgcccgg tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata
     4021 ccagccctcc cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg
     4081 gcagcagcgc cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg
     4141 ggtcattgat aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac
     4201 gggccagcga cacaccgcca aaccagataa cactaacgcc caccagcaaa atcagcagtg
     4261 aggtcagttc acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag
     4321 gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag
     4381 tattaagcgt gaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac
     4441 aggcgggatt tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg
     4501 gcgtcatcgc cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa
     4561 atcaaaatcc aagactatcc aacaaatcgt ccacctgatc ctgactggct accacaccgg
     4621 ctttgctggt atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg
     4681 actcctgttc cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa
     4741 tgacatccat catccgctta atgacctgcc cggtgagatc ctgaaaatcc tgcgccatca
     4801 tgatttccag cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt
     4861 gtcgtgtatc tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt
     4921 catcccaacg ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg
     4981 acgcctcaac actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt
     5041 acaaacgatc gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc
     5101 ccagttcccg caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat
     5161 cgccagctga atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac
     5221 atgcccagtt tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc
     5281 ttcaccacat agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct
     5341 tctgcagtca ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc
     5401 agcaattcca ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg
     5461 cctgcctgca acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat
     5521 cccagctctt tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc
     5581 aaaaatttaa gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc
     5641 cggcactaat ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga
     5701 caccacccat attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt
     5761 gcgcaagggt ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc
     5821 ccataccggt caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga
     5881 acaacacatc taccgaaggc cgatgacggt taaccgccgg gccatcgtga attttgattt
     5941 ggtaatttgc gccactacgc gacagctcca tatgccgatc gcccggcgca atataggcat
     6001 gccccggcaa gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat
     6061 taagtctgtc ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg
     6121 ccgggctgga aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg
     6181 aagcaccaat cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg
     6241 gtgctgacaa tggcttatgt gctgcaaggc tcgcctttgc tgctgtacgc accttttcag
     6301 caatcatttc gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa
     6361 aatctatcgc ccccagctcc agcgcgcgca gcgtgacttc tgaccctttg ccagtcaggg
     6421 aagaaaccat cactacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc
     6481 cgtccatccg cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca
     6541 agtcacgcgc gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt
     6601 tgatgatttc tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga
     6661 ttttgctcat ctttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg
     6721 gcgctcaagg tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag
     6781 gagcggaaca aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac
     6841 gttacgacag aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt
     6901 cagcggggca aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg
     6961 cggtcccgtc cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc
     7021 ttcatggcga taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc
     7081 aaacactttc cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct
     7141 gtacggctct tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg
     7201 acgtgcgtga tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt
     7261 cgtggtcagc gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa
     7321 gttcagataa tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt
     7381 gtaaaccatg tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg
     7441 acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa
     7501 agacgtttgc ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac
     7561 cactggcgca atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt
     7621 aaataccgcc acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac
     7681 cgccgcttct tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc
     7741 ctgtgcaacc tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat
     7801 aatgtcgttc acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt
     7861 caccagtttc gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc
     7921 ttttgctgcc tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa
     7981 accacgcccc tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt
     8041 ggtctggaaa gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt
     8101 ggcgatctcc tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt
     8161 tgtcgcggca ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac
     8221 cgtggcggtt aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc
     8281 ggtacgactt gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc
     8341 ctggcttccc ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag
     8401 actggcaaaa atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt
     8461 acccgcagca atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt
     8521 gcgcgtccac cacagcgcac tgctgatgta gattgccgca acaataatca tgctgataaa
     8581 caccagtgcc gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg
     8641 gttgatctcc agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac
     8701 cggcgcagtg aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag
     8761 atcgttgtgc cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc
     8821 gttgcccgca gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc
     8881 gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt
     8941 gcccgctttg ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc
     9001 cgcacgttgc tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc
     9061 ccaaaaagac atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa
     9121 cagcgtggtc gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac
     9181 cgcgttagta aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag
     9241 tcgtggctga gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt
     9301 gagtgggctg gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc
     9361 ttccagcgca gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac
     9421 gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga
     9481 tgccgatgca atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt
     9541 cattgtttcc ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg agacggagtc
     9601 ttcaatgagg gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac
     9661 ttcacccgcc accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc
     9721 ggcattcagc gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc
     9781 ggcaattttc ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac
     9841 tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc
     9901 gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc
     9961 cagcgcggat gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc
    10021 acgggtaccg gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa
    10081 agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat
    10141 ggtcagggta ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc
    10201 cagcggagta agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac
    10261 caccagcgcg ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt
    10321 gacgatatcg cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat
    10381 tgcattttgc attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag
    10441 ataatcaatc agttcagtta acgctgtgta atagttttta tatttttcat caatattacg
    10501 actggtagcg accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc
    10561 tgcctgcgcc aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg
    10621 attagaggaa tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag
    10681 cattaaatcc caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac
    10741 aaagctcttc tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa
    10801 cagtgcgaat acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat
    10861 aaggcacctt cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt
    10921 gctgatgcca cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca
    10981 tttcaattga ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc
    11041 catctcttcg ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag
    11101 tgcgcccagt ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg
    11161 aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac
    11221 ccgctgtccg agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg
    11281 gctgaactta attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc
    11341 tttgataaac gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg
    11401 cactttcagg atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg
    11461 gcctgacggc tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt
    11521 ttactcattc aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg
    11581 gagacatcaa caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg
    11641 gggactttgc gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat
    11701 tgatccacca gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg
    11761 gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga
    11821 tattcacccc gcacttccag cacccgctcg ccgccggcca gtggatgcag atcggcttca
    11881 cggggttgca gtgattccat gacagcattc agcggcagaa tgaaaacttc atccgcaacg
    11941 cgtacggaca tgccgtcgag gatggccaac gtcagcggca gtaaaatgcg gatcgtagtg
    12001 ccagtaccct gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta
    12061 acgacgtcca tgccgacgcc gcgcccggag acgtcggtga cctgctccgc cgtggagaag
    12121 ccaggtgcaa atatcagcat tgcaacttcg tcgtcgctca tgttttcgct gacagtcaaa
    12181 ccttgcgagg ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc
    12241 acttcaatgc aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg
    12301 tttttacctg cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt
    12361 accaggtggg tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa
    12421 ctgcccacca gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc
    12481 cggggatagc gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc
    12541 tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta
    12601 accgggtcca gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg
    12661 acgaggttaa ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg
    12721 ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta
    12781 agcactggtg gggtggatat ttttggcgag acttctactg tttcaaaagt aatctgatcg
    12841 gcttcaatca caaaacagag taccgctgtg atgtcatctt cggcaatgtc gcccggtaat
    12901 attgccgaga gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt
    12961 tcttcttcca gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc
    13021 gactgactgc gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc
    13081 actgcggatg gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata
    13141 taatcgaagc tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc
    13201 atgatgtcct tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct
    13261 cgtctggctt catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag
    13321 ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt
    13381 tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt
    13441 tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca
    13501 cctcggttcg gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc
    13561 cagggcgctt actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc
    13621 ttgtttgttc agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc
    13681 gcttaagcgc atcgttgccg ccatgccgac aacacgtaac actttgccgc tatccaaccc
    13741 gccgaccatc agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct
    13801 gtaacctttc tcaccgctgg cgtagggaaa atcatcggta tgacctgaaa ggctaatacg
    13861 gttgggaata ccgttcagta caggcgcgat ggcgcgcaga atgtcgcgca tatagggttc
    13921 gacatcggca ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg
    13981 tagaccttcc tggaccagat cgattttgag atgtggacgc aacgcccgca gtttcggatc
    14041 ggactctatc aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg
    14101 ttttttcaac tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc
    14161 atcaccaccg ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc
    14221 agtcgccagt ggagtccgga agtactccgc aatctgaatc agctcttttg ggctagagat
    14281 ggagatcagc cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc
    14341 aatcttccac gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat
    14401 aatcggatgc gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc
    14461 accgcacgca catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt
    14521 ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg
    14581 actttgacgc actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct
    14641 aatggggaaa taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt
    14701 gcgataagcg cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgacc
    14761 cccattacag ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg
    14821 ttcgccggga cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg
    14881 atttcgaagg tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca
    14941 agcatgacgc tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga
    15001 ttttcaatat cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga
    15061 taaagcagag ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc
    15121 aacggcagcg ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac
    15181 ccaatccctg caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt
    15241 ccaccggtca tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag
    15301 ataagcacga catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt
    15361 aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg
    15421 cctcaccgta tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata
    15481 tcggcaggat tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag
    15541 ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag
    15601 ttgcagctgg aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg
    15661 gttaatgcca gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag
    15721 gctttgatca ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca
    15781 ttacagaaca tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat
    15841 ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt
    15901 ccgcgactta actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat
    15961 tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg
    16021 cccttttctt gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga
    16081 gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag
    16141 taatcgtctg gtggctgtca aaacggaagt gacaaaccag ttgattggtt tctgccagct
    16201 taaccatttg cggaagagtc agtgccgcta acgttgtcgc catttcttca tttatgccga
    16261 gacgaaacat agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg
    16321 acaagttgat gtcataaatg tgtttcagca actcggaggt atgcattatt cccacccaga
    16381 ataaccaact ttatttttat gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg
    16441 gtaaaaaata attagcatta gaatagttgc gataagctgc aataagcaga accacctttt
    16501 tggtttaata tgtccttaca aatagaaatg ggtctttaca cttatctaag atttttccta
    16561 aatcgacgca actgtactcg tcactacacg cacatacaac ggagg
//
LOCUS       DAFAVF010000090        16567 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFAVF010000090 DAFAVF010000000
VERSION     DAFAVF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.090"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(65..1615)
                     /gene="cueO"
                     /locus_tag="LGK42_002910"
     CDS             complement(65..1615)
                     /gene="cueO"
                     /locus_tag="LGK42_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBK2659138.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            1781..2128
                     /locus_tag="LGK42_002911"
     CDS             1781..2128
                     /locus_tag="LGK42_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBK2659139.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            2234..3100
                     /gene="speE"
                     /locus_tag="LGK42_002912"
     CDS             2234..3100
                     /gene="speE"
                     /locus_tag="LGK42_002912"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBK2659140.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            3116..3910
                     /gene="speD"
                     /locus_tag="LGK42_002913"
     CDS             3116..3910
                     /gene="speD"
                     /locus_tag="LGK42_002913"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBK2659141.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(3948..4310)
                     /locus_tag="LGK42_002914"
     CDS             complement(3948..4310)
                     /locus_tag="LGK42_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBK2659142.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(4485..7082)
                     /gene="acnB"
                     /locus_tag="LGK42_002915"
     CDS             complement(4485..7082)
                     /gene="acnB"
                     /locus_tag="LGK42_002915"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBK2659143.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            7437..9194
                     /locus_tag="LGK42_002916"
     CDS             7437..9194
                     /locus_tag="LGK42_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBK2659144.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(9265..10689)
                     /gene="lpdA"
                     /locus_tag="LGK42_002917"
     CDS             complement(9265..10689)
                     /gene="lpdA"
                     /locus_tag="LGK42_002917"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK2659145.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(11014..12906)
                     /gene="aceF"
                     /locus_tag="LGK42_002918"
     CDS             complement(11014..12906)
                     /gene="aceF"
                     /locus_tag="LGK42_002918"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK2659146.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(12921..15584)
                     /gene="aceE"
                     /locus_tag="LGK42_002919"
     CDS             complement(12921..15584)
                     /gene="aceE"
                     /locus_tag="LGK42_002919"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBK2659147.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIANFNIDADKVNPRLA"
     gene            complement(15745..16509)
                     /gene="pdhR"
                     /locus_tag="LGK42_002920"
     CDS             complement(15745..16509)
                     /gene="pdhR"
                     /locus_tag="LGK42_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBK2659148.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         3806 a         4633 c         4097 g         4031 t
ORIGIN      
        1 gatttgcgtt tgtcatcagt ttaatgcccg gagagatccg ggcatatttc cgaatacggt
       61 ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt
      121 gcgccatata agcatgttct ttcggtgcgt tgtgattaaa cttcaccagc acttcactga
      181 cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc
      241 cattttctga caagatacgg aactgggtgc cgtgaatatg gaacggatgc agcatcatgt
      301 cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct
      361 tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc
      421 catggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg ctatgatcca
      481 tcccggccat cgcctgatcg ccatatttct ccatcagcat ctgcatcccc atcatatcga
      541 gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg
      601 caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa
      661 tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg
      721 gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct
      781 cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga ccaccgtcgc
      841 tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac
      901 agccattgag caaacgcagg cgcagccaac cacgtggggc agcgtgttgt gggtagattg
      961 caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt
     1021 gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc ggaacatcat
     1081 cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca attaccacca
     1141 gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt ggatggaacc
     1201 agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
     1261 taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg
     1321 tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt
     1381 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
     1441 aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
     1501 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc cacagcggca
     1561 aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta
     1621 tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac
     1681 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
     1741 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
     1801 agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga
     1861 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
     1921 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
     1981 gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg
     2041 cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg cccgcgattc
     2101 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
     2161 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
     2221 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
     2281 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aagatctgat
     2341 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
     2401 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
     2461 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
     2521 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtatcgtt
     2581 ctgccgtcag tatctaccca accataacgc cggtagctac gacgatccgc gctttaagct
     2641 ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
     2701 ctccgactgc accgatccta tcggtcccgg cgaaagcctt ttcacttcgg cattttatga
     2761 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
     2821 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
     2881 tggcttttat caggcggcga ttccgaccta ttacggcggt atcatgactt ttgcatgggc
     2941 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
     3001 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
     3061 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
     3121 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
     3181 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
     3241 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
     3301 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
     3361 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
     3421 agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag
     3481 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
     3541 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
     3601 cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga
     3661 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
     3721 ggtggatgtg aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
     3781 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
     3841 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
     3901 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
     3961 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
     4021 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
     4081 ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg tgtattcatg ccctgcccgt
     4141 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
     4201 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
     4261 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
     4321 tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat cgcagcctta
     4381 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
     4441 caccgggcat tgtgtcgttt atgcgcagcg cgtgcgctta cttattacac cgcagtctgg
     4501 aaaatcaccc catcggcttt ttcggtgtac tgagaaagct ggttgaagtt cagataacga
     4561 taagtatcaa cggcagtttt atctacttgc gcaacgtagg tctggtactc ttccggcgtc
     4621 ggcagtttgc caatcagcgc cgcaacagcc gccagttctg cagaagccag gaagacattc
     4681 gcgccagtcc ccagacggtt cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg
     4741 tctgctacac gcgcctggtt acccatacac agagaacagc ccgggatctc gatacgagcc
     4801 ccgctcttac cgaagacgct gtagtagcct tcttcagtca actgtgcggc gtccatacgg
     4861 gttggcggtg ccacccacag gcgggtcggc aactggcctt tatgcgcatc cagcagttta
     4921 cccgcagcac ggaagtgacc gatgttagtc atgcaagaac cgataaatac ttcgtcgatc
     4981 ttctcgccct gtaccgcaga cagcggacgc gcgtcatccg ggtcgttcgg tgcacagagg
     5041 attggctctt taatatccgc cagatcgatg tcgatcactg ccgcgtattc agcatctgca
     5101 tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat acgacgttcg
     5161 agggtacgac gatcgccata accttccgcg atcatccact tcagcaggac gatgttagag
     5221 ttcaggtatt cgatgatcgg ttctttgttc agcttgatag tacaaccagc ggcagaacgc
     5281 tcagcggacg catcggtcag ttcaaatgcc tgctcaactt tcagatccgg cagaccttca
     5341 atttccagaa tgcgaccaga gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga
     5401 ccttgtttga tcgcatacag cgggatcgcg tgtaccagat cgcgcagggt gatgcccggc
     5461 tgcattttgc ctttgaagcg caccagaacg gattccggca tatccagcgg cattacgcct
     5521 gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc gatcgggaaa
     5581 cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg gttcagccag
     5641 gagtggatta cgccgtcacc cggacgcagc gacacaccac cacggttcat aatgaagtcc
     5701 ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt atggcagaaa
     5761 gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag ttcatcacgg
     5821 gtcatcgggc cggtggtgtc ctgagagcct acagaagtca ttttcggttc gcagtacgcg
     5881 cccggacgaa tgcctttcac gccacaggca cggcctacca ttttctgcgc cagcgagaag
     5941 ccgcgatcgc tctcagcgac atctttcgcc tgacggaaca catcactgtg cggcagacca
     6001 agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcggaat acgaccgcca
     6061 gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgccg
     6121 gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caatcacgtc gcccatgttc
     6181 aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt gttaaagaag
     6241 atgggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga
     6301 atatcatcgc ccataaacca cagaacggag ttagtggcgg atttacgcga agaacccgta
     6361 cccacaacgt caccgacgta cgccagcggg aaacctttct gttgcagagc ttcgatttgc
     6421 ttgatcggac caacaacacc tggctggtct ggctcaatac cttcacgggc gtttttcagc
     6481 atcgccagcg cgtgcagtgg gatatccggg cgtgaccacg catccggtgc cggagaaagg
     6541 tcatcggtgt tagtttcgcc agtgactttg aagacggtaa cggtcagttt ttcagccagc
     6601 gccgggcgat tcaggaacca ttcggcatcc gcccaggact gcataacctg cttcgcatat
     6661 tcgttgcctg ctttcgcttt ctcttctacg tcatagaagt tatcgaacat cagcagcgtg
     6721 tgagaaagtg ctttggcagc aataggtgcc agtttggcat catccagcgc gtcgatcagc
     6781 ggatgaatgt tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc
     6841 agcagagggg atttggcttc gcctttcgcg atagcagcca ggaagcctgc tttgacatag
     6901 gcggcttcat cgacgcctgg gggaacacgg ttggttaaca gatctaacag gaattcttct
     6961 tcgcccgcgg gcgggttttt cagcagctct acaagtgcgg ccatttggtt tgcatccagg
     7021 ggtttgggcg caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc
     7081 acgacggttc tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg
     7141 tgagacagca gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt
     7201 gacgatgtgt taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga
     7261 tggtctagag attgtttccc tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct
     7321 ttctcaacga atcccaagat tagtcaaaat ttaaactacc gcctctttat actcggattc
     7381 acagcacctg cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga
     7441 aaatgacttt gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg
     7501 ccgcctctac cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat
     7561 cgacacaaca gactgcgcct gacatcaccg cagttacgct tcctgcaacg gtttccgcac
     7621 cgcccgtaac gcccgccgtc gtcaaatccg cattcagcac tgcacaaatc gatcaatggg
     7681 ttgcgcctgt cgcgctgtac cccgattctc tgctttcaca agtgttaatg gcatcaacct
     7741 atccggcaaa cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg
     7801 atgctgctat tcaggcggta tctgaccagc cgtgggacgc cagcgttaaa tcactggtgg
     7861 cctttccaca attgatggca ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg
     7921 atgcttttct ggcgcagccg caggacgtga tggactcggt acagcgattg cggcaactgg
     7981 cgcaacaaac cggctcgctg aagtcatcaa ccgaacagaa agttattacc acaacgaaga
     8041 aagctgtacc ggtaacacag acagtcacgg ctcccgtcat accatccaat accgtttcaa
     8101 ctgccagccc cgtcattaca gagcctgcaa caaccgtcat ttccattgag cccgccaatc
     8161 ctgatgtggt ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata
     8221 ctgcgtatcc gccggtttat ctgccaccac cagccggaga accgtttatt gacagctttg
     8281 tgcgcggatt cggctatagc atgggtgttg ctaccacgta cgcactattc agcagcatcg
     8341 actgggacga cgacgatcat gaccatcatc atcatgacga tgatgattat catcaccacg
     8401 atggcggtca tcgtgacggt aatggctggc aacacaacgg cgacaacatc aatatcgacg
     8461 tcaacaattt caaccgtatc accggtgagc atcttactga taagaatatg gcatggcggc
     8521 acaatccaaa ctaccgtaat ggtgtgccct atcatgatca ggatatggca aagcggtttc
     8581 atcaaaccga tgtcaacggc ggaatgagcg ccacgcagct acctgctcca acacgcgaca
     8641 gccagcgtca ggcggcagca agtcagtttc agcaacgaac acacgccgcc cccgtcatta
     8701 cacgagatac ccaacgtcag gcagcggcac agcggtttaa tgaagctgaa aactatggga
     8761 gctatgacga cttccgcgac ttcagccgtc gccaaccact gacccagcaa caaaaggacg
     8821 ccgctcgtca gcgttatcag tcggcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa
     8881 tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt attcagtcag
     8941 cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
     9001 agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa cgtcaggttt
     9061 ttcgggaaaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
     9121 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
     9181 gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
     9241 ccggatgttc cggcaaacga acacttactt cttcttcgct ttcgggttcg gcaggtcggt
     9301 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
     9361 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
     9421 acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac ggtgagattc
     9481 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
     9541 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
     9601 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
     9661 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
     9721 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
     9781 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tccacttcca cgccagcttt
     9841 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
     9901 acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
     9961 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    10021 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    10081 cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc atttccagac cgatgatacc
    10141 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    10201 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    10261 aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt
    10321 gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    10381 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    10441 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    10501 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    10561 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    10621 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    10681 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    10741 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    10801 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    10861 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    10921 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    10981 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    11041 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    11101 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    11161 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    11221 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    11281 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    11341 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    11401 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    11461 cgacaacgaa ctgttgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    11521 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    11581 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgagtaa catgcgggat
    11641 catcacccag ttacggctca ggttcgcacc agaaattttc tggatacggc ccagttccac
    11701 ttcttcgatt tcaccaaact tgctgaagtc caccttcggc cacggcagca tgcctgggat
    11761 accgccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    11821 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    11881 aacgccaaac tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt
    11941 ttctgcaaat tcagatttgc cttccgcttt cgcagccggt gctgctgccg gagcttcagc
    12001 ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc
    12061 gccttcaact tcgaagatca taatcagcga gccagttttc actttatcgc caacgttgac
    12121 tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag ctttgtcgcc
    12181 ttctacggta atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc
    12241 ggtcacttca acttcgtcac cgccgatatc cggaacgtta acttctttca cgccagccgc
    12301 tggtgcaggg gccgctgccg gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc
    12361 ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt
    12421 cactttgatc tctttcacgg tgccagcaaa cggagccgga acttccatag acgctttatc
    12481 gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat
    12541 ttcggtcact tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgctgc
    12601 agccgctggt gctgctgccg gagctgtttc tttcttctct tctgcctgag caggtgcagc
    12661 gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc
    12721 gccaacagag actttgatct ctttaacgat acccgcctgc ggagacggaa cttccataga
    12781 ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt
    12841 gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat
    12901 agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg
    12961 aaattggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg
    13021 cccagcgccg ctacaaccac gtaggaagca tcaacttcga agtggtgacg caggttctca
    13081 cggctgtcgg aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc agccggtacg
    13141 taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac tgccggagcg
    13201 tcgttcatca cctgagcgat atacggaacg cgcggagttt ccagcgggtg cagcatgttc
    13261 cagcgttcac aatcctgacc atctcgcgcc agttcggtga aggaggtcac gctataaacg
    13321 tcagaaccta cgccgtaatc tttcgccagg atctctgccg cttcacggac gtgacgcagg
    13381 atggaaccgg agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg
    13441 tagatacctt tacggatacc ttcctcagca ccttccggca ttgccggcat gtggtagttt
    13501 tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt tttcaccgta catacgctcc
    13561 agaccgtcat gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag
    13621 ttcgggatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcaccg
    13681 ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca
    13741 gccgcccagc acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaac
    13801 ggaatcatcg gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct
    13861 gcgcccaact cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa
    13921 gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc
    13981 tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga
    14041 cgatctttga tcgacttgtt cttcagcatt acgttcaggg cacgaacgaa agcgatagtg
    14101 gtagagatct ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc
    14161 tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc
    14221 tgtgcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcagtttt
    14281 tcgatatctg catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc
    14341 atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg
    14401 taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc ctgcgctttc
    14461 ttgaatgcag cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgt
    14521 tcgtcagtcc agtctgcaac cagtgctgcg gtttccggat atttaccgaa gaagtgttca
    14581 cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc
    14641 atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc
    14701 cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg
    14761 atcttgccgt taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaag
    14821 accaggttat ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg
    14881 tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca
    14941 cggtgttcca gatatttcag gaatttagcc tggtaaatag caccaatcgg ccccagaccc
    15001 atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag
    15061 aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct
    15121 tccaggaaag cacgagcgta cacgcccggg gagatgtggc cctggaagta aaccaggtcg
    15181 ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt
    15241 gcggaagact ggaaggacgc catgtggccg cccagttcga gatctttttt cgacgcacgc
    15301 agcaccgtca tgatggcgtt ccagcgaata gctgaacgaa tacggcgttc cagttccaga
    15361 ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgatacct
    15421 gtgcctgcgg ctacgtttac accgcctttg cgggcttcag caagcagttg gtcgatcaga
    15481 tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg
    15541 cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct
    15601 atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccgg aaagctcaat
    15661 aagacaggtt ctacgtttag ttgccgcgct tttatatgcg cttgatttac aacatcttct
    15721 ggataatttt taccagaaaa atcactaatt ctttcgttgc tccagacgac gcagagaacg
    15781 ctcacggcgg ctctcttcac gacttctgtc gagcaaaatt tcttcgataa aggccagatg
    15841 gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg
    15901 ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt
    15961 ctggcggaca ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac
    16021 attgtgggcc gcttcggtga cggcaatctg atactggagt acggcgtttg attccgcgtc
    16081 cagatcgcca gactgctgcg ccagctctat ggcgtggtgg agttcacgga tgcgttcctt
    16141 gtcttcatcg gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg
    16201 tgtttcgagc aagtcatact gtgactcagg atggtcggag agcagctcca ccagcggatc
    16261 gctgaagctt tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa
    16321 caagcccttc gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa
    16381 ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag
    16441 gatcaaaaac tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct
    16501 gtaggccatg agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca
    16561 tgtctgt
//
LOCUS       DAFAVF010000091        16314 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFAVF010000091 DAFAVF010000000
VERSION     DAFAVF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.091"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(10..1293)
                     /locus_tag="LGK42_002921"
     CDS             complement(10..1293)
                     /locus_tag="LGK42_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBK2659149.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1406..3340)
                     /gene="yeaG"
                     /locus_tag="LGK42_002922"
     CDS             complement(1406..3340)
                     /gene="yeaG"
                     /locus_tag="LGK42_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBK2659150.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3776..4522
                     /gene="mipA"
                     /locus_tag="LGK42_002923"
     CDS             3776..4522
                     /gene="mipA"
                     /locus_tag="LGK42_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBK2659151.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGNHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4612..5466
                     /gene="yeaE"
                     /locus_tag="LGK42_002924"
     CDS             4612..5466
                     /gene="yeaE"
                     /locus_tag="LGK42_002924"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBK2659152.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5517..6401)
                     /locus_tag="LGK42_002925"
     CDS             complement(5517..6401)
                     /locus_tag="LGK42_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBK2659153.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6485..7480)
                     /gene="gapA"
                     /locus_tag="LGK42_002926"
     CDS             complement(6485..7480)
                     /gene="gapA"
                     /locus_tag="LGK42_002926"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK2659154.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7822..8235
                     /gene="msrB"
                     /locus_tag="LGK42_002927"
     CDS             7822..8235
                     /gene="msrB"
                     /locus_tag="LGK42_002927"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK2659155.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8277..8549
                     /locus_tag="LGK42_002928"
     CDS             8277..8549
                     /locus_tag="LGK42_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBK2659156.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8710..9351)
                     /gene="pncA"
                     /locus_tag="LGK42_002929"
     CDS             complement(8710..9351)
                     /gene="pncA"
                     /locus_tag="LGK42_002929"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBK2659157.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(9362..10378)
                     /gene="ansA"
                     /locus_tag="LGK42_002930"
     CDS             complement(9362..10378)
                     /gene="ansA"
                     /locus_tag="LGK42_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK2659158.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10545..12401)
                     /gene="sppA"
                     /locus_tag="LGK42_002931"
     CDS             complement(10545..12401)
                     /gene="sppA"
                     /locus_tag="LGK42_002931"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK2659159.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVAFTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12562..13113
                     /locus_tag="LGK42_002932"
     CDS             12562..13113
                     /locus_tag="LGK42_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBK2659160.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13230..14273
                     /gene="selD"
                     /locus_tag="LGK42_002933"
     CDS             13230..14273
                     /gene="selD"
                     /locus_tag="LGK42_002933"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBK2659161.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14278..16239
                     /gene="topB"
                     /locus_tag="LGK42_002934"
     CDS             14278..16239
                     /gene="topB"
                     /locus_tag="LGK42_002934"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK2659162.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         4038 a         4052 c         4225 g         3999 t
ORIGIN      
        1 ctgatagttt tagtctttag ctgttgcatt ttgtttatga aacagttcac ggaacaccgg
       61 ataaatatca tcctggtcgc ggatgtgctg catcgcaaag ttgtcgaaag tagattgcag
      121 atgctcatat tctcgccaca atgtctgatg tgcacgacgg gtaatttcga tatagctgta
      181 ataacgaaca acaggtaata attttttcgc caggatttca tggcaaagcg gagagtcatc
      241 ggcccagtta tcgccgtccg atgcttgtgc agcgtaaata ttccactgtg ccgggttata
      301 acgctctttc actacctcat ccatcagttt cagggcgctg gaaacaatgg tgccgcctgt
      361 ttcctgcgag tagaaaaact catgttcatc gacttctttc gcctgggtat gatggcggat
      421 gtatacgact tccacgttct tatacgttct gctgaggaac agatacagca gaatataaaa
      481 acgcttagcc atatctttag tggattgatc cattgaaccg gaaacgtcca tcaggcaaaa
      541 catcactgcc tggctggagg gatcgggccg cttctcgtag ttcttgtaac gtaaatcgaa
      601 ggtgtcaata aaagggacgc gttcaatttt ggcacgtaat tctgcaattt ctttgcgcag
      661 acgttcctct tccagcagct gcgcaggttc actgttgctg atgatggcca aattctcttc
      721 cagtgcatga agttcccgcc gcttgcctgc cgtcatggct gtgcgtcgcg ccagtgagtt
      781 ctgcaaggaa cgcacaacgc tgatattggc cggaacgccg ttagcagtat aacccgcccg
      841 atgcgtttta tattcggtca gctggcgttg ttggttttgt ttcagattcg gtaaggccaa
      901 atcttcaaag agcaaatcaa gatactcatc tttcgaaatc tgaaagacaa attcatcctg
      961 accttcacca tcctggctgg cctggccctg accactgccg gaacctccgc cgccaccctg
     1021 gggacgttca attcggtcgt tctggacgaa atggtcattg cccggatgca cgcggtggcg
     1081 cagaccgcca cgcccctgat gaaacatcgg ttcgctaata tcttccgtgg gaatggatac
     1141 ggattcgccg ctgtcgacgt cagtcaccga acgcttatta atggcctcgg agatcgactg
     1201 tttaatttgc gctttataac ggcgtaaaaa acgctggcga ttcaccatgc ttttgttttt
     1261 gccgttcaga cgccggtcaa taaaccaggt catatgcccc ccgtactaca tttgccaact
     1321 gtatgctacg cctgtgttgc cgcttctcag gcccacggtt cgctgtcgtt gtaggcccgg
     1381 taacatgtgt accgggccag ggttgttaag acgatttacg tacgcgcaaa taccattcgc
     1441 acagtaaacg cacctgttta cgggtgtagc ctttttccat catacggtcg acaaagtcgt
     1501 cgtgtttctt ctgctcgtcg gttgacgttt tggcgttaaa cgagataacc ggcaacagct
     1561 cctcggtatt ggagaacatt ttcttctcga taaccgtgcg cagtttttca tagctggtcc
     1621 agttcggatt gcgtccgctg ttattcgctc tggcgcgcag tacgaagtta acaatctcgt
     1681 tgcggaaatc ttttggatta ctgatccccg ccggtttctc gattttctcc agctcggcgt
     1741 taagagactc gcggtcaaac agctgcccgg tatccggatc gcgatactcc tgatcctgaa
     1801 tccagaaatc cgcgtaggta acataacggt cgaaaatgtt ttgcccatat tcggaatagg
     1861 attcaaggta ggccgtctgg atctctttgc cgataaactc ggcatatttc gggatcagat
     1921 aacctttcag gaactccaga tagcgttctg cctgctcttg tgggaactgc tcgcgctcga
     1981 tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga
     2041 agttgaacac gcgggagagg atcttaaacg caaaacgcgt cgacagaccg ttcatccctt
     2101 catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttggga tcggtgtctt
     2161 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
     2221 taaggcgcga aagaatggaa aaacgtgaca gcgtttccag cgtgccaggg gcgcaggggg
     2281 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
     2341 gtaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
     2401 tattacggaa agtgacccat tcggattcgt tcgagtgggc gagaataatc ccgttgaacg
     2461 gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacagcg
     2521 gatgcagcac ttttatcggt gctttaaaca tctcgacaaa ttccatgatc ccttggttgg
     2581 cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
     2641 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
     2701 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
     2761 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
     2821 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
     2881 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
     2941 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     3001 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     3061 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     3121 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggt tcctgggctg
     3181 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     3241 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
     3301 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
     3361 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     3421 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     3481 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     3541 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     3601 taaagaccag ggtagccagt aacgctaaat tcatttggct gtaagcgcgg tgtcatccgc
     3661 gtcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     3721 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     3781 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     3841 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     3901 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     3961 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gacaaacttt caattaccgc
     4021 ttattggtcg ccgctttact tcaaagcgaa agacagtggc aatcaccaaa tgcgtcacct
     4081 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     4141 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca tcgtctggga
     4201 tatggcctgg ttgtatcgtt acaccaacgg cggcctgacc gtgaccccgg gtatcggtgt
     4261 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     4321 tcgcagcggt ctgcgtggct ataacccgaa cgacagctgg agcccttacc tggagctgag
     4381 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct atacccgtct
     4441 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     4501 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag
     4561 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     4621 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     4681 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc gggcattgag
     4741 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     4801 gttggggaag cattaaccgg tctgcgcgat aacgtctttc tcgtctctaa agtctatccg
     4861 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     4921 actgattatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     4981 gtggcagcga tggaaaaatt gatcgcccag ggaaaaatcc gccgctgggg cgtttctaac
     5041 cttgatcatg ctgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     5101 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     5161 cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     5221 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgctcacaa tatcagcgcg
     5281 gcacaagtat tgttggcgtg ggtaatcagc catcagggtg tgatggcgat tccaaaagcg
     5341 gccacgattg cccatgtcca acagaatgcg gctgtgcttg aggtcgaact ttcgtcagcg
     5401 gaattaacca tgctggataa agcgtatccg gcaccaaaag gaaaaactgc gctggatatg
     5461 gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac acaacattaa cgcaaattaa
     5521 cgtttcgcaa cgcgaatgga ttgcgccaga tgtgcaggtt tctctttggt cactttttgc
     5581 gtttctgaag cgtaagccgt ttctacacaa acaaatgttt tgtagccatc atccggcatg
     5641 tcgcccatgc taactgaaag cgccggtccc gggttccagc cgacaacgtt cagatgatgc
     5701 tggtggccta cggcgataat acgattcagc gcttcatcat taatcacgct gcaatcttgc
     5761 ggattcagat acacgcgatc ggtacggtca gggaaggtct gaataccgtc ggtcagtaca
     5821 tcttctttcg cgtcatttac tttatcaatg aagcgatcgc ccagcccact gacgcttacc
     5881 ttagcgatat cacccacgtt aaagtaggta tgcagggcag aggtggtttc aaattcgcca
     5941 tgtgattcaa gatcgatttc acaggtttta cccacgcgga aatgcgctaa cagcgtaaag
     6001 tcgtgcggcc agaatttttt cgtctcttcg ctttgcgtca attcaaaagt cagcgctacg
     6061 ccatcagcat cttcacgatg tgatttcagc gtccacggca ggttgcgggc aaaaccgtgc
     6121 gcaggcagac cttgttgtgc cgccggacca aaccacggcc agcaaaccgg tacgccaccg
     6181 cgaatagcga cgccattttt gaacggtgtg ttgttgctca accacagaac ttcttcttca
     6241 cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc ttttacctgg
     6301 ggatgatcga ccacaatgag gtccagttca tccagtttac gacgggagag gacaggggag
     6361 atttgttcga tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc ctttaacttc
     6421 attttatcag gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac agtgtcatct
     6481 caacttattt ggagatgtga gcgatcaggt ccagaacttt gttggagtaa ccggtttcgt
     6541 tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag agcgatacca gctttagcat
     6601 cgaacacgga agtgcaaact tcgccgttga aatcggtaga tactacgtca tcttcggtgt
     6661 agcccagaac gcctttcatt tcgccttcag cagcagcttt aacggcagct ttgatctgct
     6721 cgtaagttgc agctttttcc agacgaacgg tcaggtcaac tacggatacg ttcggggtcg
     6781 gaacgcggaa cgccatacca gtcagtttgc cattcagttc tggcagtact ttacctacag
     6841 ctttagcagc accggtagag gacgggatga tgttctggga agcgccgcgg ccgccgcgcc
     6901 agtctttgtg agacgggcca tcaacggttt tctgagtagc ggtagtagcg tgaacggtgg
     6961 tcatcagacc ttcgatgatg ccgaagttat cgttgataac tttagccagc ggagccaggc
     7021 agttggtggt gcaggaagcg ttggaaacga tgtcctggcc agcatatttg tcgaagttag
     7081 cgcctttaac gaacatcgga gtgttgtctt tagacggacc agtcataacc actttcttcg
     7141 caccagcggt gatgtgttta cgagcagttt cgtcagtcag gaacagacca gttgcttcag
     7201 cgacaacgtc aacaccaact tcgtcccatt tcaggttagc cggatcacgt tcagcggtaa
     7261 cacggatttt tttaccgtta acgatcagat gaccgtcttt cacttcaacg gtaccgtcga
     7321 aacggccgtg agtggagtca tatttcagca tgtatgccat gtaatcagcg tctaacaggt
     7381 cgttgattgc aacgatctcg atgtcagaac gtttctgagc agcacggaaa acaatgcgac
     7441 cgatacggcc aaaaccgttg atacctactt tgatagtcat atattccacc agctatttgt
     7501 tagtgaataa aaggttgcct gtaaaattac aaaaacctta cgcagcgtca agcggaatcg
     7561 tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt acgcgactga ctcgcctcac
     7621 tcttcctttg ggcttgagac cacatggggt cggcgccccg aattttaaag ggcaattacg
     7681 ataaaaatgt gattttcatc acgatttagg tgcagccatt tcgtcgagat caagtttagg
     7741 acaaaatttt gccagttggt gttaatgttt tgttagaatc agtcacgtaa tgtgagcacg
     7801 attaaagtga gatgtgagca aatggctaat aaaccttcgg cagaagaact gaaaaaaaat
     7861 ttgtccgaga tgcagtttta cgtgacgcag aatcatggga cagaaccgcc atttacgggt
     7921 cgtttactgc ataacaagcg tgacggcgta tatcactgtt tgatctgcga tgccccgctg
     7981 tttcattccg aaaccaagta tgattccggc tgtggctggc ccagtttcta cgaaccggtg
     8041 agtgaagaat ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg catagaaatt
     8101 cgttgcggta actgtgatgc ccatctgggg catgtctttc ccgacgggcc gcaaccaaca
     8161 ggcgaacgtt attgtgttaa ctctgcttct ttacgcttta ccgatggcga aaacggcgaa
     8221 gaaatcaacg gttgaaaaaa cgattcagca acttattcca caggagcgga aaaattatga
     8281 atcttgatga cattatcaac agcatgacgc ctgaggtata ccagcgtttg tcgaccgccg
     8341 ttgaactggg gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa gagaattgcc
     8401 tgcaactggt gatgctgtgg caagcccgcc ataataccga agcacagcat atgacaattg
     8461 acacaaatgg tcagatggtc atgaagagca aacagcagtt aaaagaagat tttggtatct
     8521 cagcaaagcc tattgctatg tttaagtgaa tgagttatct agtatttaag cctgttatta
     8581 agggagggca aatatacttg ttaggacgtc gtgttttagc tgcaagtagg ttaaatgcaa
     8641 cactgacaag tattatctgg cctacaatgt cattgaggaa atgtaggcca gagatgaatt
     8701 caactaatat tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc
     8761 tgctgacatc tccataaacg cgtgcgcact gtcctggggc tggatattca cgccacgaca
     8821 accatcggta atcacgttta ctttataacc taactgtaac gcgtccagca cggtaaactt
     8881 cacgcaatag tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgtaa
     8941 ccagtcatcg agagaggttt tctgccgacg gccgttatca aaaaaggcac tgtaactgtc
     9001 aactaaagga ttttcgcctt tatggaacac cgctgcgatc gctttttggt tcagtaacgg
     9061 atgtaattgt gcgccttcac tgttctgcac acagtgatct ggccagaagg tttgtggcaa
     9121 accgtcgagt tgacctggca tataaggctc tacacagtgc tgactggcaa aactgccgtg
     9181 attcgccggg tgccagtcct gactggcgat aaccgcttca ccgcgcgact ggcaccagtc
     9241 aataaggcgg ttagcgacat ccaccgtact gtcaccttcc ggcacggcga gcgcgccacc
     9301 agcacagaaa tcattttgta aatcgaccag taacagggcg cgagggggca ttgcagtctc
     9361 cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt
     9421 cagtatccag ttcctggctc agcaggtaat gcagtttggt tagcgtagct tctacagtca
     9481 tatctgcacc gccaattacg ccggcatggg cgagggcgtt accggtggca taaccaccca
     9541 tgttcacttt accggacata cattgtgtca ggttaaccac cacaataccg cgatcgctgg
     9601 cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc
     9661 gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacatca gcagaaatcc
     9721 ccggataaat cgtcactacg ccaattggtt gtggcgtgat tggatgaacg attaattccc
     9781 cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta
     9841 acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc
     9901 ggttgccgcg atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg
     9961 ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg
    10021 ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg
    10081 cagaggcggt atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt
    10141 catagtgcgc tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag
    10201 aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg catctccggg cgatggaatt
    10261 ccggcatcag cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg
    10321 aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga
    10381 tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc
    10441 tgatactatg cggataagga aatatgggtc gagacagcgg gaagaggatt cgtcgaggta
    10501 aaaaataatg tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg
    10561 caggtcaggc aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt
    10621 tcacttttaa cggtaaaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca
    10681 tctggcaaca ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc
    10741 ggttcatcaa cgtagtattc cagatgccac tgtttcactt ttgccagctc tgctgctttg
    10801 gcaaccgcat catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga
    10861 ccggtccaga cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca
    10921 tcagcaacca gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc
    10981 tgcgcttccg gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca
    11041 ccatcagtat gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag
    11101 ataccgatag aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa
    11161 atccagtaac caccagatgc cgccatgccg cccatcgata caaccacagg cttacccgct
    11221 gcccgggctg ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg
    11281 ctattaacac gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt
    11341 gccgcagtgg tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca
    11401 ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg
    11461 taataactga tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga
    11521 gctttttcga tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat
    11581 ttcgcggtat cgccaccggt tttggttaaa cccacaagca acccttgcgc gccagggaat
    11641 acctgctgag cagggatcag ccggttagcg gcaacagtat tcagatagtt ttgccacagc
    11701 tcaccaatcc agcggctgtc agcttcgcgc gctgccggtg acatatcatc acgaataaac
    11761 ggttcaacgg cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta
    11821 tccagcaacg atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct
    11881 tgcggtgaca gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag
    11941 ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg
    12001 atgtactgca tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg
    12061 gtgatattgc ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt
    12121 tcctgcagac gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga
    12181 gaactgtcgg gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg
    12241 gccgtttctt tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg
    12301 aggaaaataa agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc
    12361 catttaaaaa atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg
    12421 tcacacctgt cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca
    12481 gcaggaattg cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat
    12541 atcacgacag gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc
    12601 tcccgcctgg ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt
    12661 atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa
    12721 gggcgcgagc gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgac
    12781 aaagctatcg acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg
    12841 gcgaaatgcg aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc
    12901 gcggtcatgg cgatgcaaat ggcagccgtt gcccaaggat ttggcggcat ctggcgcagt
    12961 ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa
    13021 attgtcggtt ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc
    13081 ccggacccga ctccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt
    13141 gagcaaaggc gcaggaattc agacactctc acttatcact tcacggaatg agggttacca
    13201 tacccggatt gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat
    13261 acagccacgg agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc
    13321 acagtgagca ggcgaagttt gttgatccga atttgcttgt gggtaatgag acccgcgacg
    13381 atgcggcggt gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta
    13441 tgccgatcgt tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg
    13501 atatcttcgc gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta
    13561 acaaactttc cccagaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc
    13621 aggcgggtat tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc
    13681 tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg
    13741 gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa
    13801 aatcactgtt gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca
    13861 tcgcaggtgc gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttactggtt
    13921 ttggtctgct gggccatttg agcgaaatgt gtcagggcgc aggtgtgcag gcgcgcgttg
    13981 actatgacgc gatcccaaaa ttgccggggg ttgaagagta cattaagttg ggcgcagtgc
    14041 ctggcggcac cgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg
    14101 aagtgcgcga tctgctgtgc gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc
    14161 cggaagcaga aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga
    14221 ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taattcaatg
    14281 cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa
    14341 ccgcaccgta aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacctggtgt
    14401 atcggtcacc tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg
    14461 aatcttgcgg atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg
    14521 accaaacaac ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc
    14581 ggggacccgg atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg
    14641 gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg
    14701 gttgagcggg cgatcgaccg tcttcgttcc aacagtgagt ttgtaccgct gtgcgtttct
    14761 gcgctggcgc gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcttacacc
    14821 attctcggtc gcaatgccgg ttatcagggc gtgctttccg tcggacgcgt gcagacgccc
    14881 gtgcttgggc tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc
    14941 tttgaagtca aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa
    15001 ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg
    15061 gcggagcatg tggttaaccg catcagcggt caaccggcta ttgtcaccag ctataacgat
    15121 aaacgggaat cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg
    15181 gcaaaacgct ttggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa
    15241 acgcacaagc taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt
    15301 gccggacgcc acgcggtgat gaatgccatc agcgttcatg caccagatct gttgccgcag
    15361 ccagtggtag atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac
    15421 cacgccatca tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag
    15481 gtctataacc tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc
    15541 aagtgtgtta tcgaactgga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt
    15601 gctgaagcag gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggt
    15661 acgccattgc ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta
    15721 gagcggcaaa cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc
    15781 gggatcgcgc gctttgtgca ggacaaagat ctgaaaaaga tccttcgtgc gaccgatggt
    15841 ctggggacag aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg
    15901 accaaaaaag ggcgctatat ccactctacc gacgccggaa aagcgttatt ccattcgctg
    15961 ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc
    16021 agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag
    16081 cttattgatc aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc
    16141 agtggtggca gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt
    16201 ccgccagcag atgaagtcgg aagcggggcg atagcgtaag cgagtgaatc tttcgtgcta
    16261 ttcgagtcat attctgaaat atccagcgga tcaagaaaat tcgttggata tttt
//
LOCUS       DAFAVF010000092        16225 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFAVF010000092 DAFAVF010000000
VERSION     DAFAVF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.092"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(73..449)
                     /gene="rnpB"
                     /locus_tag="LGK42_002935"
     ncRNA           complement(73..449)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LGK42_002935"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(482..1627)
                     /gene="garK"
                     /locus_tag="LGK42_002936"
     CDS             complement(482..1627)
                     /gene="garK"
                     /locus_tag="LGK42_002936"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBK2659163.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1724..2614)
                     /gene="garR"
                     /locus_tag="LGK42_002937"
     CDS             complement(1724..2614)
                     /gene="garR"
                     /locus_tag="LGK42_002937"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBK2659164.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2644..3414)
                     /gene="garL"
                     /locus_tag="LGK42_002938"
     CDS             complement(2644..3414)
                     /gene="garL"
                     /locus_tag="LGK42_002938"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBK2659165.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3430..4764)
                     /gene="garP"
                     /locus_tag="LGK42_002939"
     CDS             complement(3430..4764)
                     /gene="garP"
                     /locus_tag="LGK42_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBK2659166.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5139..6710
                     /gene="garD"
                     /locus_tag="LGK42_002940"
     CDS             5139..6710
                     /gene="garD"
                     /locus_tag="LGK42_002940"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBK2659167.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6859..7194
                     /gene="prlF"
                     /locus_tag="LGK42_002941"
     CDS             6859..7194
                     /gene="prlF"
                     /locus_tag="LGK42_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBK2659168.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7194..7658
                     /gene="yhaV"
                     /locus_tag="LGK42_002942"
     CDS             7194..7658
                     /gene="yhaV"
                     /locus_tag="LGK42_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBK2659169.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRFFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7713..8522)
                     /gene="agaR"
                     /locus_tag="LGK42_002943"
     CDS             complement(7713..8522)
                     /gene="agaR"
                     /locus_tag="LGK42_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBK2659170.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8771..10051
                     /gene="kbaZ"
                     /locus_tag="LGK42_002944"
     CDS             8771..10051
                     /gene="kbaZ"
                     /locus_tag="LGK42_002944"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBK2659171.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10074..10547
                     /gene="agaV"
                     /locus_tag="LGK42_002945"
     CDS             10074..10547
                     /gene="agaV"
                     /locus_tag="LGK42_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBK2659172.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10558..11337
                     /locus_tag="LGK42_002946"
     CDS             10558..11337
                     /locus_tag="LGK42_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBK2659173.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11327..12205
                     /locus_tag="LGK42_002947"
     CDS             11327..12205
                     /locus_tag="LGK42_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK2659174.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12223..12657
                     /locus_tag="LGK42_002948"
     CDS             12223..12657
                     /locus_tag="LGK42_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK2659175.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12654..13787
                     /gene="nagA"
                     /locus_tag="LGK42_002949"
     CDS             12654..13787
                     /gene="nagA"
                     /locus_tag="LGK42_002949"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK2659176.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14137..15291
                     /locus_tag="LGK42_002950"
     CDS             14137..15291
                     /locus_tag="LGK42_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBK2659177.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15304..16164
                     /gene="kbaY"
                     /locus_tag="LGK42_002951"
     CDS             15304..16164
                     /gene="kbaY"
                     /locus_tag="LGK42_002951"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBK2659178.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3886 a         4328 c         4173 g         3838 t
ORIGIN      
        1 gtcgtggaca gtcattcatc tttccgctcc tccaaaagca aaaacccgcc gaagcgggtt
       61 tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac agtcattcat
      121 ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac gggccgtacc
      181 ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg actgttacca
      241 gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct tgcgcgggcc
      301 atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg ttacctggca
      361 ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga agaggacgac
      421 gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt
      481 gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt gaagcacggc
      541 agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg gtcaatacgc
      601 tgaagaccgc atcgatgcca tgctgatgta caacgccaac atcatcggtc aggctacccg
      661 caatgccaat caccggttta tggtacttct tcgccacgtt tgcaacgcca atcggtacct
      721 tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt
      781 gaatatgttc ctccagattc agcgccgtag tgacgatttc aataccactt ttcagttccg
      841 caccaagaaa cgccatcagc gccgcaccca taccgcccgc agctcctgcg ccggggacat
      901 ctttcacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag aggttattgt
      961 ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc
     1021 cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg caatctttta
     1081 agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta ccgccgccaa
     1141 aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt accatgcctg
     1201 cgccgccatc atttgtagcg ctaccgccaa tgccgataat aatgtttgtc gcgccgctct
     1261 ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggtcacgagt ggatcgcgtt
     1321 tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac gcggttttgc
     1381 catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct gtaacccagg
     1441 cgtgacgttc agaaccctgg gtggctgcaa tcatcgcttc caccgttcct tcgccaccgt
     1501 cggcaaccgg aatagaaacg tactgtgcat caggaaaaat ttcccgaaat cctttttcta
     1561 tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg gcgattacga
     1621 ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta ttcggcctgg
     1681 attccaggcc cggattgcaa tacgccatcc gggcacgacg tcattaacga gtaacttcga
     1741 ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt cctaaaccat
     1801 ctgctcgcag tgcctgcatc atctccataa ccgcagctgt gagcggcagt tgcgcgccga
     1861 cgccgtggga agtatccagc gcatttgcca gatccttaat atgcagatca atacggaagc
     1921 ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg gtactgcccg
     1981 ccagtccacc gcgaattgcc tgatacacca ggtccgggtt aacgcccgct ttggttgcca
     2041 gcgttagcgc ttctgacatc gcggcaatat tcagcgccac aatgacctga tttgccagtt
     2101 tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc atcgctttca
     2161 tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact gacagcgtac
     2221 cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca atgcctttcg
     2281 ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc atatcgatca
     2341 ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc acctctttca
     2401 catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg atcgctttag
     2461 ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct gggttacggt
     2521 cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt ttacccataa
     2581 tccccaggcc aataaaacca actttcatag tcatatcaat catctctctt gttgcggtgg
     2641 tggttatttt ttaaaggtat cagccagttt ctgagtggca gagcggaaga cgccaagatc
     2701 gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat cggcttcgac
     2761 cggcgcgaga ataccgctgg gtttgccgtg cgcactggca cggttaaaaa tgtgctgaat
     2821 tgctttttgt acatccgggt gcgaggcatt gccgagatgg cctaatgccg cagccagatc
     2881 gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatggcat cgacgttatc
     2941 tacgccctgc tgactttcta tctggaccag aatggtgatg ttcttgttcg actgagcgaa
     3001 ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc cgcgaatgcc
     3061 ttccggtggg taacgggttg atgccaccgc ctgctctgct tcctcttttg tttctacaaa
     3121 aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta ccggctcgtt
     3181 ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta actgcggaat
     3241 aaacgtggag atatcgtttg gcgcatgttc gccatccagc accagccagt caaacccagc
     3301 caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac caatttgtac
     3361 ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat tcatcgctta
     3421 tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga agaggtagca
     3481 gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga aggagtgcag
     3541 ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat tgccaaagac
     3601 gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg
     3661 ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca tgaccaccag
     3721 cgtggtgttg ttggtgtagt tacataagat gatggtggaa gccagcaaca ttcccagcac
     3781 aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat aatccgagaa
     3841 gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc
     3901 cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc aggtgaggaa
     3961 gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca tcatgcggtt
     4021 agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc tgcccggctt
     4081 tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct cttccgcaga
     4141 catacgtggg tgatctgtcg ggttatgaat caatttgatc cacagcgccg tcagcacaaa
     4201 accaatcacc cccataacgg taaagacgtg ctcccagccc caggcgaaag tcagccagcc
     4261 aagcagaggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga tggcggaggc
     4321 agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg ccgggaatga
     4381 tggcgcttcc gagaagccga gcataaagcg cataaagaac atggagatcc ctgcccaggc
     4441 cagcgggaac atatcaacaa agccttgcag gaaagtgaat agcgaccaga aaaagaggct
     4501 ataggtataa acttttttcg agccaaactt atcaagcagc cagccgccgg ggatttgcat
     4561 cagcaagtag gcccagccaa aagcagaaaa gatgtaaccc atcgaaaccg cacttaactg
     4621 caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg cgtagttaac
     4681 ggcggtaaca ataaaaataa tcagtaatat taaatagcgg gtatgcacgc ctttcttttt
     4741 ttcgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg atttttatta
     4801 ttatggggaa ggtgttattt atgagtttca tttatgccgt aacggcaatg aactcgggaa
     4861 ttagtataag cagcgcgaga ataataatca ttgtgcaaat gctaatttaa ttaatactat
     4921 ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag atttgcgctt
     4981 taccttgccg ggcggcactg caatccctga aatgattgac attgatcaca tttctgcgtt
     5041 taaactcctg acattcttat ttcacccaat gaagtcattt atttttaaat gagaccaggt
     5101 cctcatttta ataacccctg gctggagaat attgcacaat ggccaacatc gaaatcagac
     5161 aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg gcaattattg
     5221 ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tgggctggaa ttaattgaac
     5281 atattcccca ggggcataaa gtcgcattgc tggacattcc ggctaatggt gaaattattc
     5341 gttatggcga agtgattggt tacgccgtgc gtgcaatccc acgcggaagc tggatcgacg
     5401 aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg gcaaccaaag
     5461 tcccggcccc cttaccgccg ctggaaggat acacctttga gggctatcgc aatgccgatg
     5521 gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt gtggcaggcg
     5581 tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac ccgaacgtcg
     5641 atggcgtggt ggggctgaat catttgtacg gctgtggcgt ggcgattaac gcaccggcgg
     5701 cagttgtgcc tattcgtacc atccacaata tttcgctgaa ccctaacttt ggcggcgaag
     5761 taatggtgat tggtctgggt tgtgaaaagt tgcagcctga gcgcctgctg gtcggcacgg
     5821 atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc
     5881 atgtcggttt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc catctacaaa
     5941 aactgaatca acgtcagcga gaaacctgtc cggcttcaga actggttgtc ggcatgcagt
     6001 gcggtggcag cgatgccttt tctggcgtaa cggcaaaccc ggcggttggc tatgcgtctg
     6061 atctactggt gcgctgcggc gcaacggtga tgttctcaga agtcacggaa gtgcgtgacg
     6121 cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg
     6181 agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc gccaaccctt
     6241 cgccgggcaa caagaaaggc ggtctggcaa acgtggtaga gaaggcactc ggctccattg
     6301 ctaaatcggg taaaagcgca attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac
     6361 gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg caacaggtgg
     6421 cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg tacgccgtac ggcctgatgg
     6481 cggtacccgt cattaaaatg gcgacccgca ccgagctggc gaaccgctgg tttgatttaa
     6541 tggatattaa cgcgggcact atcgccaccg gcgaagaaac cattgaagag gtgggctgga
     6601 agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat
     6661 gggggctgca taaccagctg gcagtgttta acccggcacc ggtgacctga tttcttttgg
     6721 aacgtccctc gcaaaacatg gccttagtgc catgttttta ttgtttaaag cccccacgtc
     6781 cattaataat gcatttgcat taccttagtt caagcttata atttgagcag aaaacaggct
     6841 gtaaaaggac agtgaatcat gcccgctaat gctcgctctc acgctgtact gaccactgaa
     6901 tcaaaggtca cgatacgcgg acaaacaact atccccgcgc cagtgcgtga ggccttaaaa
     6961 ctgaagccag gccaggacag cattcattac gaaattctgc ctggtgggca agtctttatg
     7021 tgccgactgg gagatgaaca ggaggatcat actatgaatg catttttgcg ttttctggat
     7081 gcagatatcc agaacaaccc gcaaaaaact cgtccattca acattcaaca aggaaagaaa
     7141 cttgtcgctg gcatggacgt caacattgat gatgagattg gcgacgacga ataatggatt
     7201 ttccacaaag ggttaatggt tgggcgctat atgctcatcc ctgttttcag gaaacctacg
     7261 acgctttagt tgccgaagtc gagacattaa agggaaaaga tcctgaaaat tatcagagaa
     7321 aagccgccac aaagttattg gcggtagtcc ataaagtgat tgaggagcat atcacggtca
     7381 atccatcatc accggcattc cgtcatggca agtcgttagg ctctgggaaa aataaagact
     7441 ggtcacgggt aaaatttggt gctggtcgtt atcgtttctt ctttcgttat agtgaaaaag
     7501 agaaagtcat cattctggga tggatgaacg atgaaaacac tctgcgcacc tacggtaaaa
     7561 aaacagatgc ctataccgta ttcagcaaaa tgttaaaaag aggacatcct cctgccgact
     7621 gggaaagcct cacccaagag acagaagaaa accattgatg gtgttcacaa tgcccgccga
     7681 acgtattcgt gttgaccagc gggcaccctt tctcactccc cgaccagaat cacttcaacc
     7741 ccagcctttc gcagtccttc cagactatcc gcaggaatgc cttcatcaac aatgatcatg
     7801 tcgatacgtt gagtatcaat gatcttatgt aaactggaac gattgaattt actggaatcg
     7861 gtgaccacga tgatccgttc cgcaacttcg cacatccgac ggtttaaacg ggcttcatct
     7921 tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag aaacagcata
     7981 tcgaagtggt aattttgcag cgattgctca gcctgatcgc cgtaaaaaga ttgcgactga
     8041 cggcgcaaat gcccgccggt catcagcagc tcaacgcctt ccgcttccag caacgcatta
     8101 gccacgttca taccgttggt catcgcaatt acgtcagtgt gcttacgcat cagacgagca
     8161 atctcaaaag tggtagtccc ggaatcgagg atcacccgat gaccaggctg aatcaactca
     8221 acggcagctt tcgcaacgct gcgtttcatc gcggtgttca gcgcgctttt atcttccact
     8281 gatggctcga ctgacggcgt cgtgctatcg cagatcaacg cgccaccata ggcacgcaca
     8341 gcgatcccct gcttttccag aaacgccaga tcattgcgga tcgtcacagt agatacgcca
     8401 tacaatgccg acagatcgtt aacctgcaca ctcccttgct gtcgcagacg ctgaatgatc
     8461 tgttctcgtc gctcgctggt gccagtcact cgcttctcac ctgaagcgtc ggtattactc
     8521 atagtaagtc ctttcgtaaa actttcgttt catttcgttt tgcctattaa cgcctttcta
     8581 ttaagcaaat gcaagcccac cttgcccatt ggcgcaagct actctcgttt cagtgacttt
     8641 cattatgttt cttttgtgaa tcagatcaga aaactattat ctttcgtttt atttttatct
     8701 caccatgacg cagtatcaac tgaaacaaaa cgaaagatta atatcgcagt aatctgaact
     8761 ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg gcaaaacaaa
     8821 tggaatttat gccgtttgtt ccgcacatcc gctggtgctg gaggctgcaa tccgctacgc
     8881 cagtgcaaac caaacgccgt tactgattga agcaacctcc aatcaggtag accagttcgg
     8941 cggttatacc ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc tcgccgactc
     9001 gttgaatttc ccgcaggatg cgttgattct gggtggtgac catctggggc caaaccgctg
     9061 gcaaaacctg ccggccgctc aggcaatggc caatgccgat gatttgatta aaagctacgt
     9121 tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg tcctgtcagg acgatccgat
     9181 tcccttaact gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag tggcggaaga
     9241 aacctgtctt gaacactttg gcgaagccga tctggagtat gtcattggta ccgaagtgcc
     9301 ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc cggatgccgc
     9361 ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag caaggtttga atgccatctg
     9421 gccacgcatc attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca ccaacgttat
     9481 tgattatcag cccgccaaag cgagcgcctt aagccagatg gtcgaaaact acgaaacgct
     9541 gattttcgaa gcgcactcta ccgattatca aacgccgcaa tcgctgcgcc agctggtgat
     9601 tgaccacttt gccattctga aagttggccc agcgctgacc ttcgccctgc gtgaagctct
     9661 gttctctctg gcggcgattg aagaagaact ggtgccagcg aaagcctgtt ctggtctgcg
     9721 tcaggtgctg gaagacgtga tgctcgaccg cccggaatac tggcaaagcc actaccacgg
     9781 tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac tcggatcgcg tgcgctatta
     9841 ctggccggac agccagattg atgacgcttt cgctcatctg gttcgtaatc tggcggattc
     9901 accaattccg ctaccgctca tcagccagta tctgccgctg caatacgtga aagttcgctc
     9961 cggcgagctg caaccgacgc cacgggaact cattatcaac catattcagg acatcctggc
    10021 gcagtactac acagcctgtg aaggccaata agcaaaacaa agaggaacac gctatgccaa
    10081 atattgtttt aagccggatt gatgaacgct tgattcacgg tcaggtcggc gttcagtggg
    10141 tcggatttgc gggggcaaat ctggtgctgg tagccaacga tgaggttgcc gaagatccgg
    10201 tacaacaaaa cctgatggaa atggtactgg cagaagggat cgccgtacgt ttctggacgc
    10261 tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg acagaaaatc ctgctggttt
    10321 gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg cgttccggtg aatcgcatta
    10381 acgttggcaa tatgcactac gccaatggca aacaacaaat cgccaaaacg gtttctgtgg
    10441 atgcgggcga tatcgcagca tttaacgacc tgaaagccgc tggggtggaa tgcttcgttc
    10501 agggcgtccc gacagagcct gctgtggacc tctttaaatt actttgaggg attcatcatg
    10561 gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg cctttatcgc tggcctggat
    10621 atgtttaacg gcctaacaca tatgcaccgc ccggtggtcc tcggcccgtt ggtcgggctg
    10681 gtacttggcg atctgcatac cggaatttta accggcggta cgctggaact ggtgtggatg
    10741 gggctggccc cgctggcggg cgcacagccg cctaacgtga ttatcggtac tatcgtcggc
    10801 acggcgtttg ccattactac tggcgtgaaa cccgatgtcg cggtcggtgt cgccgtacct
    10861 ttcgctgtcg cagtacagat ggggattacc ttcctgttct cggtgatgtc tggcgtgatg
    10921 tctcgctgcg accggatggc ggagaacgcc gatactcgcg gcattgaacg cgtgaactat
    10981 ctggcactgc tggcactcgg cactttctat tttctctgcg ctttcctgcc tatctacttc
    11041 ggcgcggaac atgcaaaaac catcattgat gtcctgccgc aacgattaat cgacggcctc
    11101 ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg tgctgctgaa aatcatgatg
    11161 aaaaacgtct acatccccta cttcatcctg ggctttgttg ccgcagcctg gctcaagtta
    11221 ccggtgctgg ctattgctgc cgctgcgctg gcgatggcgc tgatcgacct gctgcgtaaa
    11281 tctcctgaac cgactcaacc tgcggcacag aaagaggaat ttgaagatgg catctaatca
    11341 aaccaccctg ccgaacgtca gtgaaaacga agaaacattg ctgactggcg tcaatgaaaa
    11401 cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa aaagatatca accgcgtcgc
    11461 ctggcgttcc atgctgttac aagcgtcttt taactacgaa cgtatgcagg cttctggctg
    11521 gttgtacggt ctgctgcctg ctctgaaaaa gatccacact aataaacgcg acctggcgcg
    11581 cgccatgaag gggcatatgg gtttcttcaa tacccatccg tttctggtga catttgttat
    11641 cggcattatc cttgcgatgg agcgttctaa gcaggacgtt aacagtattc agagcaccaa
    11701 aattgccgtc ggtgcgccgc tcggcgggat tggcgatgca atgttctggc taacgctact
    11761 gccgatttgt ggcgggattg gagccagtct ggcattgcaa ggctccattc ttggcgcagt
    11821 cgtctttatt ctgctgttca acgtggtaca cctggggctg cgttttggtc tggcgcatta
    11881 tgcttaccgc atgggcgtgg cggcgattcc actcattaaa gcgaatacca aaaaagtcgg
    11941 ccatgctgcg tctatcgttg ggatgacggt aatcggcgcg ctggtggcaa cctatgttcg
    12001 tttaagcacc acgctggaaa tcacagcggg cgacgcagtg gttaagttac agactgatgt
    12061 tattgacaaa ctgatgcctg ccttcttacc gctggtctac accttgacca tgttctggct
    12121 ggtacgccgc ggctggagtc cgctgcgtct gattgcagtt accgtggttc tcggcatcgt
    12181 cggtaaattc tgccatttcc tttaaataca atgaggtatt agatgttaag tattattttg
    12241 acagggcatg gcgggtttgc cagcggcatg gaaaaagcaa tgaagcaaat tctcggtgag
    12301 caatcgcagt ttatcgccat cgattttccg gaaacctcaa gcaccgcgct gcttacatcg
    12361 cagcttgaag aggccatcgc ccaactggat tgtgaagacg gcattgtttt tctgacggat
    12421 ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg cgatgcaaaa accgggctgt
    12481 gaagtgatca ccggcaccaa tttgcaactg ctactggaga tggtgctgga gcgcgaaggg
    12541 ttaagcggtg aagaattccg tgtacaggcg ctggagtgtg gacaccgagg gctgaccagt
    12601 ctggtggacg agttaggccg ctgccatgaa gaatgtccgg tcgaggaagg aatatgacac
    12661 acgttctgcg cgccagaagg ctgctgactg aagagggatg gctcgatgac catcagttgc
    12721 gtattgctga cggtgtcatc gcagcaatcg aaccgattcc agtgggcgtg actgaacgcg
    12781 atgcggaact gctctgcccc gcttacatcg acacccatgt acacggtggt gcgggcgttg
    12841 atgtaatgga tgacgcgccg gatgtactcg acaagctggc aatgcacaag gcacgcgaag
    12901 gtgtcggcag ttggttgccg actaccgtaa ccgcgccgct taacagcatt catgcggcgc
    12961 tgaaacgtat tgctcaacgt tgccaacgcg gcggacctgg tgcgcaagtg ctggggagtt
    13021 atctcgaagg accgtacttc acgccgcaga ataaaggcgc gcatccgccg gaattgtttc
    13081 gcgagcttga aattgccgag ctggatcaat tgattgccgt ttctcagcac accttacgcg
    13141 tggtagcgct ggcaccggaa aaagaggggg cattgcaggc catccgccat cttaaacagc
    13201 aaaatgtacg agtgatgctg gggcatagcg cggcgacctg gcaacaaact cgcgccgcgt
    13261 ttgatgctgg tgccgacggc ctggtgcatt gctataacgg gatgacaggt ttacatcacc
    13321 gcgaaccggg aatggttggc gcgggattaa cggacaagcg cgcctggctg gaactgatag
    13381 ccgatggtca tcatgtgcat ccggcggcga tgtcgctgtg ttgttgctgt gcaaaagaga
    13441 gaatcgtgct gatcaccgac gcgatgcagg cagccgggat gccggatggt cgctatacgt
    13501 tatgtggcga agaagtgcag atgcacggtg gcgttgtccg taccgcgtcc ggtgggctgg
    13561 cgggcagtac gctgtctgtt gatgcggcag tgcgcaacat ggtcgagttg acgggcgtaa
    13621 cgcctgcgga agccattcat atggcatcgc tgcatccggc gcgaatgctg ggtgttgatg
    13681 gtgttctggg atcgcttaaa ccgggcaaac gcgccagcat cgttgcgctg gatagcgggc
    13741 tgcatgtgca acaaatctgg attcagagtc aattagcttc gttttgatag tttgctcctt
    13801 tattgggcct tcacttcccc cgtaaggcct ttctttttct ttcgttttga tctgtgcagc
    13861 ggtgtcggat gcgacgctaa cgcgtcttat gcgacctacg gtacgccgca tccggcaatc
    13921 ggtgaacata gcgacgtcat tacgcaattc ttaatcttcc cttgtctttt ctttcaacga
    13981 tcacagattt cgttttattt cctttttctc cattgaactt tcagtttctt ttctatagat
    14041 tttaatcaac gaaagacatc accaagtgaa atgaaacgaa aggcaagtga aagcgacaac
    14101 gcccgacgtc aagttcatca gactaaggat tgagttatgc cagaaaatta cacccctgct
    14161 gccgccgcaa ccggtacatg gactgaagaa gagatccgcc atcagcctcg cgcatggatc
    14221 cgttcactca ccaacatcga cgcgctacgt tccgcgctca ataacttcct tgaaccgtta
    14281 ctgcgcaaag agaatctgcg gatcatcctg accggagccg gaacgtcggc atttatcggt
    14341 gacatcatcg cgccgtggct cgccagccat accggtaaaa acttcagcgc cgtaccgacc
    14401 accgatctgg tcaccaatcc gatggactac ctgaatccag ctcatccgct gctgttgatc
    14461 tccttcggtc gatccggcaa cagcccggaa agcgtcgctg ccgtggaact ggcaaatcaa
    14521 tttgtaccgg aatgctatca cctgccgatc acctgcaacg aagcgggcgc tctttaccaa
    14581 aacgcgatca acagcgataa cgcatttgcc ctgctgatgc ccgcagaaac gcacgatcgc
    14641 ggctttgcga tgaccagcag cattaccacc atgatggcca gctgcctcgc ggttttcgca
    14701 cctgagacga tcaacagcca aaccttccgc gacgtggcgg atcgttgcca ggcgatcctg
    14761 acctcactgg gcgatttcag cgaaggtgtg tttggttacg caccgtggaa acggatcgtt
    14821 tatctcggca gcggtggctt acagggcgca gcacgcgagt cggcgctgaa agtgctggaa
    14881 ctgacggcgg gtaaactggc ggccttttat gattctccaa ccggattccg tcatggacca
    14941 aaatcgctgg tcgatgacga aacgctggtg gtggtatttg tctccagcca cccttacacc
    15001 cgtcagtatg atcttgatct gctggctgaa cttcgccgtg acaaccaggc aatgcgtgta
    15061 atcgccatcg ccgcggaaag cagcgacatc gtcgctgccg gtccacatat catcctgcca
    15121 ccgtcacgtc actttatcga cgttgagcag gcattttgct tcctgatgta cgcccagacg
    15181 tttgcactga tgcagtcgct gcacatgggc aatacgccgg ataccccatc agccagtggc
    15241 accgttaacc gcgtggtgca aggcgtaatc attcatccgt ggcaggcata agaggatcgc
    15301 attatgagca ttatctccac taaatatctg ttacaggacg cccaggccaa tggctacgcg
    15361 gtgcctgctt ttaacattca taacgccgag acgatccaag cgatcctcga agtgtgcagt
    15421 gaaatgcgat cgccggtgat cctcgccgga acgccgggga cctttaaaca tatcgcgctg
    15481 gaagagatct acgccctgtg cagcgcctat tccacaacct acaacatgcc actggcgctg
    15541 catctcgacc accacgaatc gctggatgat attcgccgta aagtccacgc aggtgtgcgc
    15601 agtgcaatga tcgacggcag ccacttcccg tttgccgaga acgtgaagct ggtgaaatcg
    15661 gttgttgact tctgccactc acaggattgc agcgtggaag cagaactggg ccgcctgggc
    15721 ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat tcctgaccga tccacaagaa
    15781 gctaaacgct ttgtcgaact gactggcgtc gacagcctgg cggtagcgat tggtacggcg
    15841 cacggcttat acagcaaaac gccgaagatt gatttccagc ggctggcgga aattcgtgaa
    15901 gtggtggatg ttcctctggt gctacatggt gccagcgatg ttccagatga atttgtccgt
    15961 cgcactattg aacttggcgt cacaaaagtg aacgttgcca cagaattaaa aatagccttc
    16021 gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg gtaatgatcc tcgttattat
    16081 atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa ataaaattaa tgtctgtggt
    16141 tcagcgaatc gaatttcagc ataatcatta gttttttatg atttatccca atgtacttcc
    16201 cgattaatat cggggagtgc cttaa
//
LOCUS       DAFAVF010000093        16138 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFAVF010000093 DAFAVF010000000
VERSION     DAFAVF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.093"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..704)
                     /locus_tag="LGK42_002952"
     CDS             complement(<1..704)
                     /locus_tag="LGK42_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBK2659179.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLARE"
     gene            complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="LGK42_002953"
     CDS             complement(882..1793)
                     /gene="gsiD"
                     /locus_tag="LGK42_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBK2659180.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="LGK42_002954"
     CDS             complement(1796..2716)
                     /gene="gsiC"
                     /locus_tag="LGK42_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBK2659181.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="LGK42_002955"
     CDS             complement(2734..4272)
                     /gene="gsiB"
                     /locus_tag="LGK42_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBK2659182.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="LGK42_002956"
     CDS             complement(4292..6163)
                     /gene="gsiA"
                     /locus_tag="LGK42_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBK2659183.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTVQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="LGK42_002957"
     CDS             complement(6150..7115)
                     /gene="iaaA"
                     /locus_tag="LGK42_002957"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK2659184.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7319..8554
                     /gene="moeA"
                     /locus_tag="LGK42_002958"
     CDS             7319..8554
                     /gene="moeA"
                     /locus_tag="LGK42_002958"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBK2659185.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8554..9303
                     /gene="moeB"
                     /locus_tag="LGK42_002959"
     CDS             8554..9303
                     /gene="moeB"
                     /locus_tag="LGK42_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBK2659186.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9482..10144)
                     /gene="fsa"
                     /locus_tag="LGK42_002960"
     CDS             complement(9482..10144)
                     /gene="fsa"
                     /locus_tag="LGK42_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK2659187.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQAVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10275..11174
                     /locus_tag="LGK42_002961"
     CDS             10275..11174
                     /locus_tag="LGK42_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBK2659188.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11180..13612
                     /locus_tag="LGK42_002962"
     CDS             11180..13612
                     /locus_tag="LGK42_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBK2659189.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13758..14573
                     /gene="ybiV"
                     /locus_tag="LGK42_002963"
     CDS             13758..14573
                     /gene="ybiV"
                     /locus_tag="LGK42_002963"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBK2659190.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14725..15990
                     /locus_tag="LGK42_002964"
     CDS             14725..15990
                     /locus_tag="LGK42_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBK2659191.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTADQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3936 a         4426 c         4072 g         3704 t
ORIGIN      
        1 ttttcacgag cgagttgatt cttggcaatg atctcatgac cttgtttcaa aaatagaact
       61 tcatcattag cgctgatttg atcaagatac tcgttcagat accgccagtt taaatcgaca
      121 tgaattttat ctaccgctaa caaatcgtga tctttgccgt tgatacgtag cgtaaaagag
      181 atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag
      241 tcatctgaag ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc
      301 cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca
      361 ttcgccctcc gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct
      421 aaaaatggca tcatttccat tttcagaatc acggcgcgtt gaatctcctc tgtatttttt
      481 aataaataca gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag
      541 ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat
      601 accagttgat agttaatgat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat
      661 aaaattatca gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca
      721 aaataactat ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc
      781 aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattg
      841 cctgtgaggt gattaccctt tcaagcaata ttcaaacgta actatccttt aattttcgga
      901 tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt cagaaaaata
      961 gccagggccg gaaaaacagc gacatgcggc gcgataacca tatccgctcg tgcctcattg
     1021 agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg
     1081 gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa acaccacgat agacgagacg
     1141 gtcccaggca agatatgacg caataaaatg gtcatatcgc tggcaccaat actgcgtgct
     1201 gactcaataa aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac
     1261 gcggggatgg aaaaaatggc gactgcgata atcacgttag caatgccgct tcccaacacc
     1321 gcaacaacag cgatcgccag taaaataccc gggaaggcaa acagcacatc gcaaatgcgc
     1381 atgatcaacc gatcccacca gccttcataa tatccagcca acaagcccag caacgtcccg
     1441 atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc
     1501 aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa
     1561 ggtccgttat tcagattgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag
     1621 cgtgcaaaaa tggccaccac aatcaataaa ataacgaata atgcggcggt catcgccata
     1681 tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgcacctg gtcaggtttg
     1741 accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact
     1801 tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa
     1861 taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta
     1921 cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aactttctcc accacgatag
     1981 aaccaccgag caaaaagcca aactgtaagc ccatcatggt cactaccggg atcatcgcgt
     2041 tacgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac
     2101 gcatataatc ttcgcttaaa acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg
     2161 ctgccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc
     2221 ccacggtagg cagccagccc agttcaacgg agaatacctg aattaaaagc atccccagcg
     2281 caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac
     2341 gattacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca
     2401 tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaaccg
     2461 gacgacgcga caccatcgac aggccaaaat ccccctgcac agcattgctg atatagtgcc
     2521 agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct
     2581 gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atatgcacaa
     2641 ataaaaacac cagcaccgag acgataaaca gcgtcggaat caaccccagt aagcgtttga
     2701 taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct
     2761 gaagccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag
     2821 tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct gcgccgcctt
     2881 atacaagcgg gtcttttccg ccggatcatt agttttcagt gcctgagcca ggaagtcgtc
     2941 cacctgttta ttgctgtaaa atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc
     3001 gaacagtggc gatagcgccc agtccgcttc gccggttgaa gccgaccagc cagtgtagaa
     3061 catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc
     3121 atccatcgcg gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg
     3181 cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt
     3241 gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt
     3301 ataactttgc gcgtaggcga tacttggcgg taccacacca gtagctggcg ttgcatagcc
     3361 cgcaaaggca actttcacca gcgccggacg gttaatggcg taattcagcg cctcacggac
     3421 cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct gcataattga
     3481 cggactggcc atcaactcga tatttttgtt tttctccagc agtgtggcct gctcgtaggg
     3541 aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc
     3601 cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc agtaacccgc
     3661 gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat acggtccggt
     3721 tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg gtgaaatcat
     3781 cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt tgagggtaat
     3841 ctttactgtt gttggatcga tcgcctccgt tttggcaata ttcttataca ggttatagcg
     3901 tttaagatga ttcgccggat cgctggcccg gtccagattc gctttcaccg ccgcggcgtt
     3961 gaaatcggtg ccatcctgga atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc
     4021 gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct ctttatccag
     4081 accgaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtgt cattcgcgtc
     4141 atacggatcg agcgtggtga aattcgatcc taccgccacc accacatctt tggcagcgaa
     4201 tgcacaagat gccatcaacg ctgtcgcaat gcccagcgcc actaacccac tacggtgtac
     4261 agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt
     4321 gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt
     4381 cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt
     4441 gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgctgcg
     4501 ggttttcgaa gaccgcgcgc cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg
     4561 ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac agatacgcaa
     4621 tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat
     4681 ccagcgccga gacggcttcg tcggcaatga tcactttcgg attcaatgcc aacgcgcgag
     4741 caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt
     4801 caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgct tctttacctg
     4861 gcaataaacc gtgtacacgc agcggttcga taatcgaatc accgatggtc tgacgtgggt
     4921 ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt
     4981 tgccgggtga caaggtatcg attctctgac cgttaaagat aatttcgccg ccctgcgatt
     5041 cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca
     5101 gcgatagcgt ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc
     5161 gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc
     5221 gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggggcc tgtttcgctg
     5281 gatgttcaag cgatatcaac gggaaacgtc ggggataatc taaccctttc atcgcaccaa
     5341 gttgcggaac agcagctaac agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct
     5401 gttcgacggt acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa
     5461 tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt
     5521 gcaatacttt gattaattgc aggatctgcg cctgaacagt gacatccagc gcggtggttg
     5581 gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct
     5641 ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa
     5701 tgcgtacctg atccagcatc cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct
     5761 gatgcagacg aattgattcg gcaatctgtt cacccacagt aaataccggg ttcagcgatg
     5821 tcatcggttc ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc atttgtgcag
     5881 cgctctgctc gctaagttca atcacttcgc gactgcgccg ctgcaacagc attttatcgc
     5941 actgtactaa accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca gtcactgact
     6001 taccggagcc ggattcgcca acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga
     6061 gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc agattttcaa
     6121 ccgccagcac attaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca
     6181 cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg
     6241 gtgttaaacg gtagcgcgac attcccttca tggtcgatag cgattaagcc accgctaccg
     6301 ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg
     6361 ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgccc
     6421 gtgccggtac aagaaaccgc cacactggcg ttattggcgt agcatccggc acccactaag
     6481 ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc
     6541 gccaaattgc cgtctaaatc caacgccacg gcccccacgg tgcccatttt ctgtttttca
     6601 tccagtggcg caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt
     6661 tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat gccacgagca
     6721 aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc
     6781 agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc
     6841 agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca
     6901 gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc
     6961 acatccagcg cactttcgcc cgcttccagc attttctgcc cggtttcaac aatggcagac
     7021 aacgcctcaa tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct
     7081 gcgccaccat gaattgcaat gactgctttg cccatagtat tcgtccatta agggtggctc
     7141 gccacttttg ctataaatat cagaattttt atgaattact cattcaattt ttgaatatag
     7201 aaagatggat atggcatgta aaggcaggag ccaacgctac gcccgaggtg gcatacagtc
     7261 tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat
     7321 ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt
     7381 caccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc gtattctggc
     7441 gagcgatgtc gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa tggacggcta
     7501 cgcggtgcgt ttagccgata ttgcctccag gcaaccgctg cccgttgccg gtaaatcctt
     7561 tgccggtcag ccgtaccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg
     7621 tgcgccggtg ccggaaggct gcgaagcagt ggtgatgcag gagcagactg aacaaacgga
     7681 caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga
     7741 agatatctct gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta ccgctgaact
     7801 gccagtgatt gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc
     7861 gcttttttct accggtgatg aactccagtt gcccggtcag ccactgggcg atggccaaat
     7921 ctacgatacc aaccgtctcg ccgtacacct gatgttagaa cagttgggat gcgaggtaat
     7981 caacttaggg attatccgcg acgatccaca tgccctgcgc gccgcattta ttgaagccga
     8041 cagccaggcg gatgtggtga tcagttccgg cggtgtttca gtgggtgaag cggattacac
     8101 caaaaccatt cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccggg
     8161 taaaccgttc gcgttcggta aactttctaa tagctggttc tgcggcctgc cgggcaaccc
     8221 ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg
     8281 caacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa
     8341 aacgccagga cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact
     8401 tgaagtgacg accaccggac atcagggttc acatatattt agctccttta gtctcggcaa
     8461 ctgctttatc gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt
     8521 agaaccgttt aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg
     8581 ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgcta
     8641 aaagattctc gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc ttcgcagtat
     8701 ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctcg
     8761 aatctgcaac gccagacact gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc
     8821 gcccgttacg ccctgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg
     8881 ctagatgacg cagaacttgc agcattgatt gctgaacacg atctggtgct cgactgtacg
     8941 gataacgttg cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg
     9001 gtttccggcg cggcaattcg tatggaaggg caaatcacgg tcttcactta tcaggacggc
     9061 gaaccgtgct atcgctgcct tagccgtttg tttggcgaga acgcattaac ctgcgtagaa
     9121 gcaggcgtga tggcaccgtt gatcggcgta attggttcgt tgcaggcaat ggaagcgatc
     9181 aaactgctgg caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg
     9241 acctgtcagt ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag
     9301 taattgccac ctccaggcct gataagacgc gacaagcgtc gcatcaggca tttgctccag
     9361 acgccagatg cggcgtgaac gccttatctg gcctacggtt cgggcacaga tttccaagcg
     9421 cggcaggcat cggtgcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
     9481 gttaaatcga cgttctgcca aacgctccca gccagtcctg ttcaaacttc gccacagcgg
     9541 catcaaccgc cggatagcta atcatctgtt gtgccacatc cagtggcagc gtaattgatt
     9601 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc gctgccagca
     9661 ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gcctgaatgc
     9721 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
     9781 gcgccgacag cagcccttgt gctgcgccat ataccgcggt tcccagcgtt ggaatccctt
     9841 ccgcttttaa catcttaata gctgccagcc cctcggcggt caccggaact ttcaccacga
     9901 tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca gcagtggtag
     9961 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca
    10021 caacatccag cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca
    10081 gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt
    10141 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
    10201 tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa
    10261 acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta
    10321 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
    10381 aaagccgcgc ccgcacgcag gatctgctgt atgacgcacg actgtgtctg gaaggctgcg
    10441 agctgtgcgc taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc
    10501 atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgt ccgacacagg
    10561 cattaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg
    10621 ataaaccgtt ttatgatcgc agcggcggcg gtttaacgct ttcgggtggt gaacccttta
    10681 tgcagccgga aatggcgatg gcgctactgc aagccagcca cgaggccggc attcatactg
    10741 cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
    10801 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
    10861 gtaacgccgc cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
    10921 ttatccgcgt gccgctgatt cagggcttta atgccgacga aacctctgta aaagccatta
    10981 ccgattttgc cgccgacgag ctgcacgttg gcgaaattca ttttctgccc taccacacgc
    11041 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg
    11101 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
    11161 ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa
    11221 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca
    11281 gcactatacc gagatgtatc aacaacatct cgataagccg atcccggtac gtcgcgcgct
    11341 ggcactggcg catcacctgg cgaatcgcac catctggatc aaacacgatg agttgatcat
    11401 tggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg
    11461 gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga
    11521 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga
    11581 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa
    11641 agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct
    11701 ggaaaaaggt cttgatggtc tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct
    11761 gacggtgctg gaagatttac atggcgaaca attcctgaaa gcgattgata tcgtgctggt
    11821 ggcagtcagt gaacacattg aacgtttcgc tgccctggcg cgtgaaatgg ctgctaccga
    11881 aactcgcgaa agccgtcgcg atgaactgct gacgatggca gaaaactgcg atcttatcgc
    11941 ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgttacttca tccaattgat
    12001 tttgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc agtatctcta
    12061 cccgtactat cgccgcgacg ttgaactcaa ccagacgctg gatcgcgaac acgccatcga
    12121 gatgctacat agctgctggc tgaaactgct ggaagtgaac aagatccgct ccggctcaca
    12181 ctcaaaagcc tctgcgggaa gtccgctgta tcagaacgtt accattggcg gacaaaatct
    12241 ggttgatggt caaccaatgg atgcagtgaa tcccctctct tacgcgatcc tcgaatcctg
    12301 cggtcgcctg cgttccactc agcctaacct cagcgtgcgt taccatgcag gaatgagtaa
    12361 tgatttcctc gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa
    12421 caacgacgaa atcgtgatcc cggaatttat taaactcggt attgaaccgc aggacgctta
    12481 cgactacgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg
    12541 taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg
    12601 tgatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt
    12661 taacaatttc gatgaagtaa tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa
    12721 atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct
    12781 ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc
    12841 gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca acagcctggc
    12901 ggcagtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc tggctgccgc
    12961 actggcggat gacttcgatg gcctgactca tgagcaactg cgtcagcggc tgattaacgg
    13021 tgcgccgaag tacggcaacg acgatgatac tgtcgatacg ctgctggctc gcgcttatca
    13081 gacctatatc gacgaactga aacagtacca taatccgcgc tacggtcgtg gtccggttgg
    13141 cggcaactat tacgcgggta cgtcatctat ctccgctaac gtaccgtttg gcgcgcaaac
    13201 tatggcaacg ccggacgggc gtaaagccca caccccgctg gcagaaggcg ctagtccggc
    13261 ctctggtact gaccatctcg gccctactgc ggtcattggc tcggtgggta aactgcctac
    13321 ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccggcaa cgctggagaa
    13381 cgaatctgac aagcagaagc tgatgatcct gctgcgcacc ttcttcgagg tgcataaagg
    13441 ctggcatatt cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc
    13501 ggatcagtac cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct
    13561 ctctccagac gctcaggacg atatcatcgc tcgtactgaa catatgctgt aatctcttcg
    13621 gcccggctgc ggcgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca
    13681 ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc
    13741 gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac ggtacttttc
    13801 ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa
    13861 agcgcggcat tgagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct
    13921 ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac
    13981 atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg
    14041 agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca
    14101 gcgaaaatgc ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaag
    14161 ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccag
    14221 atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctcgatggc attatgaaac
    14281 ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg
    14341 gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg
    14401 acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg
    14461 ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg
    14521 cgctgaatgt gattcaggcg gtgctggata acacatcccc ttttaacagc tgacctctct
    14581 accggagccg tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca
    14641 tcaaacttgc attaacttat ttataactta tattgtcaca tgcaaatcag aatggtttcg
    14701 aatgaaagtt aatgagaggt ggttatggct tctactttta ccagcgacac attgcctgcc
    14761 gatcacaaag cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc
    14821 cagcagatct ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca
    14881 ctcaaagcac agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt
    14941 catgttactg cagatcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc
    15001 catttccccc gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctggaccgc
    15061 aaccgctttg acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct
    15121 tcacgtcccg agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt
    15181 gaagaaatgg cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga
    15241 aagcaatggt ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccgtccg
    15301 cccggaacga cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg
    15361 ctgcttccag cgtatcagcg cgttttcgcc aacgtcttta atggcaatct ggcgcaatat
    15421 gatccctggc atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc
    15481 aaatgttccg tgtttcggac attccagggc tggacagcgc tctctgatat gctgcctggt
    15541 caggggctgc tgcacgtcgt gcccattcct gaagccatgg cgtacgtact gttacgtccg
    15601 ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtattgccg
    15661 gtatcagagc aatggcatcc actgttgatt gaggcgttaa ccagcattcc aaaactcgaa
    15721 gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat
    15781 caacagggct ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt
    15841 gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg
    15901 cgcgaggact atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac
    15961 ggtaagcgag cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt
    16021 aagaaaatcg attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg
    16081 cggcattaac gccttatctg gcctacaaag tcctgcagat tcaatatatt acaggtac
//
LOCUS       DAFAVF010000094        16061 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFAVF010000094 DAFAVF010000000
VERSION     DAFAVF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.094"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(108..1757)
                     /gene="pgi"
                     /locus_tag="LGK42_002965"
     CDS             complement(108..1757)
                     /gene="pgi"
                     /locus_tag="LGK42_002965"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK2659192.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2282..3631
                     /gene="lysC"
                     /locus_tag="LGK42_002966"
     CDS             2282..3631
                     /gene="lysC"
                     /locus_tag="LGK42_002966"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBK2659193.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3776..4702
                     /locus_tag="LGK42_002967"
     CDS             3776..4702
                     /locus_tag="LGK42_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK2659194.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4738..5529)
                     /locus_tag="LGK42_002968"
     CDS             complement(4738..5529)
                     /locus_tag="LGK42_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK2659195.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5535..6662)
                     /locus_tag="LGK42_002969"
     CDS             complement(5535..6662)
                     /locus_tag="LGK42_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HBK2659196.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            7192..7464
                     /locus_tag="LGK42_002970"
     CDS             7192..7464
                     /locus_tag="LGK42_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBK2659197.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(7465..8337)
                     /gene="rluF"
                     /locus_tag="LGK42_002971"
     CDS             complement(7465..8337)
                     /gene="rluF"
                     /locus_tag="LGK42_002971"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBK2659198.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            8549..9238
                     /gene="pepE"
                     /locus_tag="LGK42_002972"
     CDS             8549..9238
                     /gene="pepE"
                     /locus_tag="LGK42_002972"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBK2659199.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(9329..10960)
                     /locus_tag="LGK42_002973"
     CDS             complement(9329..10960)
                     /locus_tag="LGK42_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBK2659200.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(11180..14863)
                     /gene="metH"
                     /locus_tag="LGK42_002974"
     CDS             complement(11180..14863)
                     /gene="metH"
                     /locus_tag="LGK42_002974"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK2659201.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            15063..15887
                     /gene="iclR"
                     /locus_tag="LGK42_002975"
     CDS             15063..15887
                     /gene="iclR"
                     /locus_tag="LGK42_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBK2659202.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3852 a         4292 c         3986 g         3931 t
ORIGIN      
        1 ttttaggatt aacctcacgg tatgatttcc gttaaatcac agacaagggg atttatctga
       61 taaaacaatg ccgacataac gtcggcattg ttattaaggt ttttaaatta accgcgccac
      121 gctttatagc ggttaatcag accattggtc gagctatcgt ggctgctgat ttctttatca
      181 tctttcagct ctggcagaat acggtttgcc agttgtttac ccagctccac gccccactgg
      241 tcgaaggtga agatgttcag gatcacgccc tgagtaaaga ttttgtgctc atacagcgca
      301 atcaacgcac ccaggctgaa cggagtgatt tcacgcagca ggatggagtt ggtcgggcgg
      361 ttaccttcga acactttgaa cggcaccacg tagtcaagcg ttgccggatc tttaccctga
      421 tcgcgatatt cctgctcaac cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg
      481 gcgaagaagt tagacagcag tttctggtga tgatcagaga gcgggttatg ggtgatagcc
      541 ggagcgatga aatcgcacgg taccattttg gttccctggt ggatcagctg gtagaacgcg
      601 tgctgaccgt tagtgcctgg ttcaccccag ataatcgggc cagtctggta atccacaacg
      661 ttaccgttac ggtcaacata cttaccgttg gactccatat tgccctgctg gaagtacgcc
      721 gcgaaacggt gcatatactg gtcatacggc agaatcgcct cagtttccgc accaaagaaa
      781 ttgttgtacc agatgccaat cagcgccagc agtacaggca ggtttttctc ggcaggcgtg
      841 gtggagaaat gcttgtccat cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag
      901 ccgatggaga gaacaatcga caggccaatc gctgaccaca aagagtaacg gccgccgacc
      961 cagtcccaga actcgaacat gttggcagta tcaataccaa actcgccaac ggctttggca
     1021 ttggttgaga gcgccgcaaa gtgtttcgca acgtgttttt catcacctgc cgctttcagg
     1081 aaccagtcac gcgcgctatg ggcgttggtc atggtttcct gagtggtgaa ggttttagat
     1141 gctaccagga acagcgtggt ttccgggttt acttttttca gcacttccgc gatgtgagtc
     1201 ccatcgacgt tagaaacaaa gtgcatgttc aggtgatttt tgtacggacg cagtgcttcg
     1261 gtcaccatgt acgggccgag gtcagaaccg ccgatcccga tgttcactac gtcagtgatt
     1321 gctttgccgg tataaccctt ccactcaccg gagataatcg cttccgagaa ggttttcatc
     1381 ttctccagca ccgcgttgac ttccggcatt acgtctttgc catcaaccaa aatcggggta
     1441 ttgctacggt tacgcagcgc tacgtgcagc acagcgcggt tttcagtgcg gttgatcttc
     1501 tcgccagaga acatcgactt aatcgcgccc gccagatcgc actctttcgc cagatcttgt
     1561 aatttcgcca gcgtctcttc agtgatgcgg tttttggagt aatccaccag catctgatcg
     1621 ccgaaggttg cggagaactt agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc
     1681 gtaacgtctt tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt
     1741 ggattgatgt ttttcattag caatactctt ctgattttga gaattgtgac tttggaagat
     1801 tgtagcgcca gtcacagaaa aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt
     1861 aaacccttag cgcagtgaag catttattag ctgaactact gaccgccagg agtggatgaa
     1921 aaatccgcat gaccccgtcg ttgacaaccg ccccgctcac cctttattta taaatgtact
     1981 acctgcgcta gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt ggaggagcca
     2041 gtcctgtgat aacacctgag ggggtgcatc gccgaggtga ttgaacggct ggccacgttc
     2101 atcatcggct acaggggctg aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg
     2161 tttcgcaagt ggtggagcac ttctgggtga aaatagtagc gaagtatcgc tctgcgccca
     2221 cccgtcttcc gctcttccct tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt
     2281 tatgtctgaa attgttgtct ccaaatttgg cggtaccagc gtagctgatt ttgacgccat
     2341 gaaccgcagc gctgatattg tgctttctga tgccaacgtg cgtttagtag tcctctcggc
     2401 ttctgctggt atcactaatc tgctggtcgc tttagcggaa gggctggaac ctggcgagcg
     2461 attcgaaaaa ctcgacgcta ttcgcaacat ccagtttgcc attctggaac gtctgcgtta
     2521 cccgaacgtt atccgtgaag agattgaacg tctgctggag aacattactg ttctggcaga
     2581 agcggcggcg ctggcaacgt ctccggcgct gacagatgag ctggtcagcc acggcgagct
     2641 gatgtcgacc ctgctgtttg tcgaaatcct gcgcgaacgc gatgttcagg cacagtggtt
     2701 tgatgtacgt aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc cagatgtagc
     2761 cgcgctggcg gaactggccg cgctgcagct gctcccacgc ctcaatgacg gcttagtgat
     2821 cacccaggga tttatcggta gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg
     2881 cagcgattat acggcagcct tgctggcgga ggctttacac gcatctcgtg ttgatatctg
     2941 gaccgacgtc ccgggcatct acaccaccga tccacgcgta gtttccgcag caaaacgcat
     3001 tgatgaaatc gcgtttgccg aagcggcaga gatggcaact tttggtgcaa aagtactgca
     3061 tccggcaacg ttgctacccg cagtacgcag cgatatcccg gtctttgtcg gctccagcaa
     3121 agacccacgc gcaggtggta cgctggtgtg caataaaact gaaaatccgc cgctgttccg
     3181 cgcgctggcg cttcgtcgca atcagactct gctcactttg cacagcctga atatgctgca
     3241 ttctcgcggc ttcctcgcgg aagttttcgg catcctcgcg cggcataata tttcggtaga
     3301 cttaatcacc acgtcagaag tgagcgtggc attaaccctt gataccaccg gttcaacctc
     3361 cactggcgat acgttgctga cgcaatctct gctgatggag ctttccgcac tgtgccgggt
     3421 ggaagtagaa gaaggtctgg cgctggtcgc gttgattggc aatgacctgt caaaagcctg
     3481 cggcgttggc aaagaggtat tcggcgtact ggaaccgttc aacattcgca tgatttgtta
     3541 cggcgcatcc agccataacc tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt
     3601 gcagaagctg cattttaatt tgtttgagta atatttccct actggcgtat aacgctcacc
     3661 gattgttata cgccatcctc actgaaaaca tagattaaat aatatctgaa aagcaaaatc
     3721 actcctgtag aacagtatta taatcaccga aaaatatcat ttaacaggag tgttaatggc
     3781 tactttaatg gaaaaagatg cacttttaaa cggagccagt cagtgcatcg cgtttttatc
     3841 aaatatcatt gataattgct cagtttcgtc gcatcaggat tcaggcgatg ctctcaagcg
     3901 attagtcagc tatcgcgatt atctttattc tacccccgct gaacttgttg attttacaca
     3961 aggtaagatt ctccttcagc aggtaagaac tcaatatcag catgaattca acaatactac
     4021 gcattcagaa aataaagcat cttttgattc tatttggcag cgtctgacaa atcatgaagt
     4081 cacaccgcaa cagcatccca ttggttttgt tttaggtggg caacccggag caggtaaatc
     4141 atctctgatt gaattagcca aaagagagac aaaagataat ataatgatta ttaacggtga
     4201 tgattttcga tttctacacc ctgattttaa ttatatctac caaaattatg gggatgattt
     4261 tgttacccat acagcgaaat tttccggcga aacggtcgaa cgtgcaattg agagagcgat
     4321 agtcagtaaa ctcaacattg ttgtcgaagg aacatttcgt aatgccgcga cgccgttaca
     4381 aacattaaaa aaactcaaag atgcaggcta ccaaacagaa gtcatgataa aaacaacctc
     4441 agcggcatta agctgggaaa gcaccaatga acggtatagt aaggacaaag aggcgggaaa
     4501 tattgcccga aaagtggata aaaaccacca tgatatcgtc accggtttgc tggcagaaaa
     4561 tgcccgcaag gtgtttgctt cgaacctgtc agataagttt gcggtgtatt cgcgtgagaa
     4621 gatgatattt agttcacaag cggcaacgaa tgacgatatt gcaacactta tacagaatga
     4681 aatcagcggg aatacacaat aataataaga gctgatggcg tgaaataacg ccgggcatca
     4741 cgcccggcgc attaccgtct ggtacattaa gctatccagt tccttaacgt ccccttctct
     4801 gctatccggt aacagcggtg gaatttcccc acgcgccagt tccgcacgaa taaacgcatg
     4861 taacaatgga cggcgcggac catattccgc ttctcctgcg cgctgtttac gttccagtaa
     4921 ttcgtctatt tctgcacgta acggcgcatc aagttcgctt cccgccagta gctcagcaaa
     4981 gcgcattggc ggtacgcctt ttcccgcttc aacccaacgc acggccagca gcggacgcag
     5041 cacgtaaaag tattttttca gccgcacttc gtcgccttgt aaataaccgc gaaagttttt
     5101 ccgcgccatc gaataataat gccaacgcgc acgcagcgga gaaaaccact tcggcaccat
     5161 tgccttaagc gccgttatcg tttcttcatc ttgttgatag accacaggag aatccagcca
     5221 ttctatcagc gtcggattcg cacctttcaa taatcccagc gccttgcgcc actcccagcc
     5281 tgaaacatct aactcgtcgt caataggcaa ctcaataaca tcgcgcggcg cctctactcg
     5341 cagataccat tccagcgggt gaacatacaa aaaccgtaca tcgtagtcgc tgtcgggcga
     5401 ggcaaatccc cagccgcggc tccctgattc acaggcgtac agtactttca catcataccg
     5461 ccgttcaatt tctcttaact gctgcgatac cctttctcgc atcgcagcac tgactccatt
     5521 taatggcatg ttactcaccc tttgatatta accaacccat gcagaacctg atccagaccg
     5581 ccaaatacag agatgttatc gatccgttca gccactctct ccagcgtttc cagttctttt
     5641 aagcgtaacg ctaccgggtt gttttccatg actttagcgg tattgagtaa cgaacgcgtc
     5701 gctgccgttt cttcacgtcg gcgaatcaca ttggcctgcg cggatttctc cgcctcaacc
     5761 agctgcgcaa ggatattttt catatcgcca ggcaggacga tatctttcac ccctaacgag
     5821 gcaatctcca taccaaatgg caacatgcgc gatttcacct gttcgctgac aacgtcatca
     5881 atgacctgtt tatcttccag caattcatcc aacgtacgcg tacctaccgc ttcacgcagg
     5941 gcaaattgca attcccgata caaatgatca atcggcttcg ttaactgact aaatgccagc
     6001 aacacgtcgc tgtagcgcca gttagcggcg aggttaatcc gcagattgac cttatctttc
     6061 gttaagattt cttgcccgct gacttccagt acctgcaagc gggtgtcaac cacttcagcg
     6121 tcaaccagat ggttaatttt ccagtatgcg gtcagccccg gcggtaacag cgcctgcgtt
     6181 tcaccatcga ttttcagcac gcctacatgc caggccggaa cctgcaccgt gagtatcgca
     6241 tcgcggcctt taactgcccc tttgcgccgt ggttgcaata ccgcattcat cacctccgtt
     6301 tgtacctgga cttgctgcgt attcatccgc accagtgtca aatcatcctc tacacgccag
     6361 tacaaacggc gcgttgatgg cggcaggatc tcctgcaaaa taccgtccat atacaacgcc
     6421 ccggcttcaa tctccgacaa gtctgccacc aggcaatatt tctccaccca atcaggctga
     6481 aagcggcgca aataatccgc cagcacgtcg ggaacttcgc tgccatcgag cgtaatcagc
     6541 aagacttctg gcgtatttaa ccagggtaaa agatgctcgc ctgcatgtag cactttgtag
     6601 taatcgccgt ttctggagag taatgccagc tgatcttttc ttacgcttat ttttttaatc
     6661 attatctatt ccttgcttaa cagccgtcgc ggaagcagac acgaagcgac aggggaattc
     6721 ccttttgcct gttacccggc atctttatcg gcgacgttat cggggatcgc tgactgcgca
     6781 atccctcact ttttatttaa taagttaaaa ccagtattca tttcaggaat gaatgtggga
     6841 atcgaaccca taattggcgt caaaggctga cggaacacgc ccggaaaaca gcctggccgt
     6901 tcttggtgtg ccgacggggt ttgaccccct ttgtaattca gtttcaacgc gtacgagata
     6961 ttgcgagaag cgtgccagaa ttgagaaaaa attattaacc aaatgaaaaa taaagattta
     7021 ataaattatt tttgtttgta gagggaaaac ttttcgctaa atttgtatct gtatttatcg
     7081 tcaattatca agaaagataa atatttaaga agagaacaaa ggaatattat cagttcagct
     7141 cgcaaacgaa ttcccgtaca ctgaactctc tggctcactg agggcaccat catggcactc
     7201 ccccgtatta cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa aacactgctg
     7261 gatcgggcgc ggattgcgca tggtcgcgta ctgaccaact ccgaaaccaa cagcattaag
     7321 aaagagtaca tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa agcccgccag
     7381 ttgaagaaaa agcaggctta taaaccggat cctgaagcgt catttagctg gtcagcaaat
     7441 acgtcgacgc gcggcaggcg ctgattagcg ccccttcttt ttacgccccg gagaggtaaa
     7501 acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca tcttaacgac
     7561 tggacgtttg atgcccgctg ttttcggttt ggccttcact ttaggttttg cctcggaaga
     7621 ggaattttcg atgagcttaa agaggtcgat taactcatca tcagttaaat cgcgccactc
     7681 acccagcgga atgccgctta aactgacgtt cataatgcgc gtacgttcca gctttttcac
     7741 ttcatagccg aaatgctcgc acatgcgccg gatctgacgg ttcagcccct gcaccagggt
     7801 aatgcggaag acaaacggcg cttctttttt gactttacac tttttagtca ctgtcccgag
     7861 gattggcacc cccgccccca taccacgaat aaattcatcg gtaatcggtt tatcgaccgt
     7921 aaccagatac tctttctcat gatcgttacc cgcacggagg atcttattca ccagatcgcc
     7981 gtgattggtg aggaaaatca gcccctggga gtctttatcc aggcggccaa tcgggaacac
     8041 gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgccatctt cggtggtgct
     8101 gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggctt cccgaggctc
     8161 aatcaactga ccgtttactt tcacaacgtc gccgggtttc acctgatcgc caatggtggc
     8221 tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt cgcggcgtga
     8281 gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg gcagcataaa
     8341 ttctcctgta aaaaacggaa tataccgtac ctttgggttg ataaaaaata gtcatgagcg
     8401 aactatttgt tatttccatt ggcttgaatt cgttacctgc tcaaatcact ttccagaagc
     8461 aatgtgcgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctc
     8521 ccgtgatttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
     8581 gccgggtaaa gcctggctgg aacatgcact gccgctaatt gctgaacagt tgcagggtcg
     8641 ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag acctgggatg attacacagc
     8701 gaaaacggct gcggttctcg ctccgctggg tgtttctgtc accggtattc atagcgttgt
     8761 cgatcccgtt gccgcgattg aaaatgctga gatcgtgatt gtcggcggcg ggaatacttt
     8821 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggttaa
     8881 acgtggcgct ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg
     8941 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc
     9001 gttgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
     9061 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctacc
     9121 ggaaggtaac tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac
     9181 ttatgtgttt aaggctggtg aagaagcggt gccgctagaa gctggtcacc gtttttaagt
     9241 gaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg
     9301 tcttatcagg cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg
     9361 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc
     9421 ccagtaagcc taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc
     9481 gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg aaagcgatgc ttactacggc
     9541 gcaaacgacg agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat
     9601 tactgagcaa ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc
     9661 gccgtgctgc cagtgatttg tcggcaattt cactccccat gcgctcgacg atatcggagg
     9721 cctgttcaag gttgagcgac atttcgatga tctctgccca gcggcgcgac tcttcctccg
     9781 ccagctcttc ttttggcatc cgcgccagat acagcttaat ggcggtgtag agaacgttga
     9841 tatcatctgc cagcttacgc agctctttct cctgccgtgg ctcaccgtgc atcactttat
     9901 tcaacccttc catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt
     9961 tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgttta ggccgtagct
    10021 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
    10081 caaatggcag catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca
    10141 gttccgcttt tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca
    10201 ggataatcag gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg
    10261 cggcattggc ggcactgttg ttgagcatcg ccagcagacc ggaaccgagg ttagcaccaa
    10321 tcaccagaca gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcggtca
    10381 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
    10441 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
    10501 tcggcgttac ggcctgcaca atcaactcca gcgctagcaa aatcagccca agaccaatac
    10561 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
    10621 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
    10681 ccgtcccgac atctgcaccc agcacaatga ccagagccgg tgcgagggct accagatcct
    10741 gagcgacaaa cgaggtcacc agcatggtgg tggcattact gctctgtacc agtgcggtaa
    10801 cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac
    10861 gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca
    10921 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
    10981 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgttg actggcatct
    11041 ttactgtgcc ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat
    11101 aagtgtttca tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga
    11161 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
    11221 caacttcggt aacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa
    11281 tttgcgctac cgcgtaatac ttgctgtccg ggtggctgaa gtaccaaccc gaaaccgatg
    11341 caccaggcca catggcgaaa gattctgtga gtttcatgcc agtgtgtttt tccacttcca
    11401 gcagctccca gatggtggct ttttccgtat gttccgggca ggccggatag cccggtgccg
    11461 gacggatgcc ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg
    11521 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta
    11581 aacggtcggc aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
    11641 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
    11701 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcccgttt
    11761 tttcggtctg ttgacgcaga tggtggctga cgttgatcac atgagtgcgc gtttcgtcac
    11821 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg
    11881 gattcagcgt tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
    11941 gctgcgcctc aacgcctacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
    12001 accaggtcat aaagaacggt gtccagtcga tgtaattgcg cagcgtttcg atgctggctt
    12061 cgacttcctg cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag
    12121 cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgcggc ttcttgcgcc
    12181 cgtgctgaat acgtacggtt tcgtactcct tgcgggtacg agcgacaaaa tcatcacgct
    12241 gggtatcgga aagcagcgcc gccaccacac caacggtacg cgatgcgttc tgcacataca
    12301 ccgtcgggcc gctgtagttc tgctcgattt tcaccgccgt gtgcgctttt gaggtggtcg
    12361 cgccgccaat caatagcgga atagtgaagc cctgacgctc catctctttc gccacgttaa
    12421 ccatctcgtc cagcgacggc gtgataagcc ccgaaagtcc aatcagatca gcattcactt
    12481 ctttagcggt acggagaatt ttttccgcag gcaccataac gccgagatcg acaatttcgt
    12541 agttgttaca ttgcagcacc acaccaacga tatttttacc gatgtcgtgg acgtcgccct
    12601 tcacggtggc gatcaccatc ttgccgttgg ttttaccctg ctctttgctg gcttcaataa
    12661 acggttcgag atacgccact gcctgtttca tgacgcgcgc cgatttgacc acctgtggca
    12721 ggaacatttt cccttcgcca aacagatcgc cgaccacgtt cataccgtcc atcagcgggc
    12781 cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc ttcttcggta tcctgctcga
    12841 taaactcggt aatgccttta accagcgagt attccagacg tttattcact tcccacgagc
    12901 gccactccgc ctgctgggcg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg
    12961 caagctccag taaacgctca gtgccatcgt cgcgacggtt aagaatcaca tcttccaccg
    13021 cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc
    13081 ccatatccat gccattgcga atagcgtagt agaggaacac cgcgtgaatg gcttcgcgca
    13141 ccggatcgtt gccacggaat gagaaagaaa cgttagatac accgccggaa atcagcgcgt
    13201 gcggcagttc gcgtttgata tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt
    13261 gctcttcaat gccggttgcg accgcgaaga tgttcgggtc gaagatgata tcttctggcg
    13321 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg
    13381 cgcgggtatc ggcctgtccc tgttcgtcaa aggccattac caccactgcc gcaccgtaac
    13441 gacgcaagag cttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt
    13501 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
    13561 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
    13621 gcaccatcgc cgcttcggcg tcgagcatcc cttcatccat gttgatatcg ataatctgcg
    13681 caccgttttc cacctgctgg cgggccacat ccagtgcctc gctgtatttc tcttctttga
    13741 tcaggcgctt gaacttagcg gaaccggtga cgttggtgcg ttcacccacg ttcacaaaca
    13801 ggctatcttc gccaatgttc agcggctcca ggccggacaa acggcaggct acggggattt
    13861 ccggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg
    13921 gcgtggtgcc acagcagccg ccgacgatat tgagaaaacc cgcttgcgcc cattcacgta
    13981 tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg
    14041 cgttcgggtg cgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc
    14101 gcagttcatc tggccccagc gcacagttca ggccaaaggt cagagcttcg gcgtggcgca
    14161 atgagttgta aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
    14221 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
    14281 caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
    14341 caccaccttc caccagcgct ttggtggact ctcgataagc cgccaccagc ccgtcaaaag
    14401 tgatattacg aaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggttg
    14461 ggccgagaac accggcaacg tagcgcggtt tctccggggt gcgcgcggtc cactcatcag
    14521 cacaggcgcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
    14581 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
    14641 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttactg agtaccagca
    14701 ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
    14761 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac
    14821 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
    14881 tcaacccaga tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa
    14941 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    15001 tattgcatta gctaacaata aaaatgaaaa tgatttccac gatacagaaa aaggagactg
    15061 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    15121 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    15181 ccaatggcag tgtggcactc acggaactgg cgcaacaagc cgggttaccc aattccacga
    15241 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtt ggcgaactgg
    15301 gacactgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    15361 atttgttagc aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg
    15421 tcaatatggc ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt
    15481 gtacccacct gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttccg
    15541 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    15601 gcaaagggtt acatgcctat acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag
    15661 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc
    15721 tacgttgcct tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcaa
    15781 tttccggacc gatttcacgt attaccgatg accgcgtgac cgagtttggc gcaatggtga
    15841 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt ttttctggcg
    15901 ggcagaggca atattctgcc cgtcatatct gagtggcaat agaataaggg tgtctgttaa
    15961 tcgcattgac gccaaaataa cttattgtca tacacttcgc acacttttat taactcagca
    16021 ttatttttaa acatcaaacc acttaattat taacaaaatc g
//
LOCUS       DAFAVF010000095        15869 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFAVF010000095 DAFAVF010000000
VERSION     DAFAVF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.095"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..152)
                     /locus_tag="LGK42_002976"
     CDS             complement(<1..152)
                     /locus_tag="LGK42_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2659203.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIP"
     gene            160..1122
                     /gene="tnpA"
                     /locus_tag="LGK42_002977"
                     /pseudo
     CDS             160..1122
                     /gene="tnpA"
                     /locus_tag="LGK42_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1331..1969)
                     /gene="leuE"
                     /locus_tag="LGK42_002978"
     CDS             complement(1331..1969)
                     /gene="leuE"
                     /locus_tag="LGK42_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBK2659204.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2096..3019)
                     /gene="dmlR"
                     /locus_tag="LGK42_002979"
     CDS             complement(2096..3019)
                     /gene="dmlR"
                     /locus_tag="LGK42_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBK2659205.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3122..4207
                     /gene="dmlA"
                     /locus_tag="LGK42_002980"
     CDS             3122..4207
                     /gene="dmlA"
                     /locus_tag="LGK42_002980"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBK2659206.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4458..6068
                     /gene="yeaV"
                     /locus_tag="LGK42_002981"
     CDS             4458..6068
                     /gene="yeaV"
                     /locus_tag="LGK42_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBK2659207.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6100..7224
                     /gene="yeaW"
                     /locus_tag="LGK42_002982"
     CDS             6100..7224
                     /gene="yeaW"
                     /locus_tag="LGK42_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBK2659208.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7280..8245
                     /gene="yeaX"
                     /locus_tag="LGK42_002983"
     CDS             7280..8245
                     /gene="yeaX"
                     /locus_tag="LGK42_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBK2659209.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLKENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8299..9414)
                     /gene="rnd"
                     /locus_tag="LGK42_002984"
     CDS             complement(8299..9414)
                     /gene="rnd"
                     /locus_tag="LGK42_002984"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBK2659210.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9496..11187)
                     /gene="fadD"
                     /locus_tag="LGK42_002985"
     CDS             complement(9496..11187)
                     /gene="fadD"
                     /locus_tag="LGK42_002985"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBK2659211.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFL
                     RIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDP
                     SLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11392..11973)
                     /gene="yeaY"
                     /locus_tag="LGK42_002986"
     CDS             complement(11392..11973)
                     /gene="yeaY"
                     /locus_tag="LGK42_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBK2659212.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12013..12708)
                     /gene="tsaB"
                     /locus_tag="LGK42_002987"
     CDS             complement(12013..12708)
                     /gene="tsaB"
                     /locus_tag="LGK42_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK2659213.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12766..14676)
                     /locus_tag="LGK42_002988"
     CDS             complement(12766..14676)
                     /locus_tag="LGK42_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK2659214.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14808..15152
                     /locus_tag="LGK42_002989"
     CDS             14808..15152
                     /locus_tag="LGK42_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBK2659215.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15515..>15869
                     /locus_tag="LGK42_002990"
     CDS             15515..>15869
                     /locus_tag="LGK42_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBK2659216.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKAL"
BASE COUNT         3909 a         4135 c         3885 g         3940 t
ORIGIN      
        1 gggatgtatt tattaccggc ctcatgattc tcattctgaa gcgccagagc acgattgaaa
       61 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgatc acctggtctt
      121 aactggaatt taaatgcctg tagtcgtttc acaggccaaa tgatactttg gtctgtgaaa
      181 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      241 tttgtcacca gatacagacg tcagattttt gaccatgacg cgacagaaaa actacgcgct
      301 tacttttcaa atgtatgtgc tgattttgaa gctgaacttg ttgaaatgga tggcgaacca
      361 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac
      421 agcctcaaag gcgtatcggg taggtgactg cgacgagatc gaccagatat tgcagtcagg
      481 tattactaca aaggcgtttt gtggagtgct ggctattttg ccagtagctg cggaggtgcg
      541 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtca ggtggaaaac
      601 cgcgccttat atccctgccc tgaaggatgg ggttttacgg cgcaccggat aagaacgcac
      661 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
      721 cgtctctgtt tcataatcga cagaaacgcc attgctgtta ttggtgacgg tcacggtata
      781 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
      841 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
      901 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
      961 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     1021 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     1081 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     1141 ctgtgggatt tgcagcatcc tattccctcc ttcggggaat gcgctgaaaa cggtttattc
     1201 cagccgtttc agggtacgcc tgataatatg cattttaaat accatttatt ggttactttt
     1261 tagcaccata tcagagaaaa atcagggagg attacaagtg gggagcccgc gacaacgcgg
     1321 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     1381 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     1441 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     1501 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     1561 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     1621 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     1681 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     1741 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     1801 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     1861 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     1921 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     1981 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     2041 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaatc attaatcagg
     2101 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
     2161 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     2221 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     2281 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     2341 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     2401 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcagaaa
     2461 gatcggttaa atgtttcggc gcaccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     2521 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg tgcgatgtca tcgccaatgc
     2581 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     2641 gtaattccag ttgcggatac gccttcgcca gcgctgataa cgccggagcc accacctgtc
     2701 gtccaaaacc aaagctgctg ataatacgca gcatcccctg gggcacctga cgcacgtcag
     2761 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     2821 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     2881 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     2941 ccgccacggc ggcaaaacca gcccgacgag ccaccagcat aaagacgcgc aaatcattca
     3001 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     3061 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     3121 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
     3181 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg acttgagttt
     3241 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
     3301 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
     3361 ggataccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
     3421 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
     3481 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
     3541 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
     3601 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
     3661 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
     3721 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
     3781 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
     3841 ggtggcgtcc aatttatttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
     3901 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
     3961 gccagtccac ggttccgcgc cggatatcta cgggaaaaac attgctaacc ctatcgccac
     4021 gatttgggcc ggtgcaatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
     4081 agcgcataac ggtattctgg cagcaattga agaagtgatt gctcacgggc cgaaaacacc
     4141 tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
     4201 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
     4261 tttgcagtaa ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
     4321 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
     4381 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
     4441 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
     4501 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
     4561 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
     4621 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
     4681 ttcgtcttgg cgaaggaaaa ccggaataca gcacactctc ctggctgttt atgtttattt
     4741 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
     4801 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
     4861 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
     4921 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
     4981 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
     5041 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
     5101 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
     5161 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
     5221 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggcc
     5281 caaccgaatt tattaccaac agcatcatca atgccatcgg tctgaccacg caaaacttcc
     5341 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
     5401 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
     5461 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
     5521 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
     5581 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
     5641 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
     5701 tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt acgcgtaatc
     5761 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
     5821 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
     5881 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
     5941 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
     6001 caccggttga agccctggaa aaaacgccag tgctccctgc gggaaccgta ttcaaaggcg
     6061 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
     6121 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
     6181 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
     6241 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
     6301 attggtgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
     6361 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
     6421 acctgcccgt atcacgcatg ggcattcaaa ctcgatggca acctggccca tgcacgtaac
     6481 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
     6541 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcaa
     6601 ttacccggcc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
     6661 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
     6721 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
     6781 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
     6841 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
     6901 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
     6961 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
     7021 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
     7081 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
     7141 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
     7201 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
     7261 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagccaggt
     7321 tgaacccctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccatt
     7381 acctgcgttt actggaggaa gtcacgtcat tgtgcagatg agcgatggtg ataaccagta
     7441 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
     7501 tcggctgaag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
     7561 caatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
     7621 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagct
     7681 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
     7741 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcatc
     7801 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
     7861 ttatacctgt ggccccgagg cgctaattga agcggtaaga agtgaagctg cgcgtctgga
     7921 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
     7981 atttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
     8041 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
     8101 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcaatattt
     8161 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
     8221 taaacgcctg gtgttggatt tgtagtttgc cgaagccgga tgtggcgctg agcgcgccca
     8281 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
     8341 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
     8401 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
     8461 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
     8521 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
     8581 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
     8641 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgctaccg accacaaatg
     8701 ctcttcacgc acgacaaagt tcaccgccag atcgcgctct cgcgccttgc gcagtcgcca
     8761 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
     8821 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
     8881 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
     8941 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
     9001 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
     9061 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
     9121 gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
     9181 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
     9241 gtcggtgatc ccgagaggat caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
     9301 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
     9361 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
     9421 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
     9481 cgactgactt aacactcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
     9541 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
     9601 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
     9661 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
     9721 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
     9781 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
     9841 gaacccttct tcatccatca ccgcaatgtc gccggtgtgt aaccagccat ttttgatgat
     9901 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
     9961 aagctcaccc ggttgacccg gtggtacttc attatcatta tcatcatcat ccaccagttt
    10021 ggcttccgtc gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg
    10081 gttaacgctg accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc
    10141 tgtcagtttc acccaacgct ctgccaccac ttgctgcact ggcatccctc cgcctgcgga
    10201 aagatgcaga ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa
    10261 caaggtgtta acgcccgtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc
    10321 tggaatatcg cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag
    10381 gcagttaatg gtcagggcaa aaatgtgata cagcggcagc gccgtcacca ccagctcttt
    10441 gcccggatgc aacagcggac cataggtggc gttgacctgt tccaggttcg ccagcatatt
    10501 gcggtgagtc agcatcgcgc ctttcgccac acctgtggtg ccgccggtgt attgcaggaa
    10561 ggctaaatct tccggcacca gttcgggttt gacgtactgc atccggtagc cgttatgcag
    10621 tgcgctacga aatgaaatgg catctggcag atggtatttc ggcaccaaac gcttgatgta
    10681 tttaacaacg aaattgacta ccgtgccttt tgccgtagat agctgatcgc ccatacgggt
    10741 cagaattacg tgctgaacgg cggttttatc aaccactttt tccagtgtgt gagcaaagtt
    10801 agacacgata acaatcgccg atgcgccgct atcgttaagc tgatgctcaa gctcacgcgg
    10861 ggtatacaac gggttaacgt ttacgacgat catcccggca cgcaaaatgc caaacagcgc
    10921 caccggatat tgcaataaat taggcatcat caacgcaacg cgatcgcctt tcttcagccc
    10981 caacccttgt tgcaaataag cggcaaacgc gcgactgcgt tcttccagct tgcggaaggt
    11041 cattacctcc cccatattca caaacgcagg ctgatcggcg tagcgcgcga ccgactgctc
    11101 aaacatatct accagagatt gataacggtc agggttgatc tccgtcggaa cgtccgcggg
    11161 ataacggtta agccaaacct tcttcaattc ttcacctcta aaatgcgtgt tcgtcgtcat
    11221 cgcaacccca aatgatatac atgccgttaa cataatatta actcatcata ccagcttgat
    11281 aattacccaa cgaaaaggtt gcgaagcgcg tcactattta tttttatctt taccgtaaga
    11341 atgcagaaac agcggaccag ccgctgtttc tttttcttta aaaacaagca attattcagt
    11401 tacaactgtt tgtactctcg cggggccggg attataccag ccccatccgc catagccata
    11461 gggccagcca cgaccgccat aaaaccatgg atcaatcggc tgaggcggca taatcacctg
    11521 ctgggttaaa tgccaacgtt tgtaacccgt tacttgcatc accataaatt tatagggcgt
    11581 gttgccgatt tttccgtcaa ccgcaccagt gattggcccg actaccgtaa ccagttgtcc
    11641 acggaaatcc accgggtcca gaaaaccgtt catatcggca taaatgcgac cgcgagaagg
    11701 ttctcccagc gtcggtctgg ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt
    11761 tttcccttgc tggttttgta ccgcaaccac tttgccacca aagcgtgcct cctgaccaac
    11821 gtacagctgc ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg gactgctgcc
    11881 tttaatggcg tccggcacag tgacacaacc gctcagcatt agcgcaaacg tacctgccag
    11941 tatgcctttg ataacattct tttgaaccgc catggtgcga ctcctttttt tctcagggca
    12001 tactcttaag attcattctt tgcccggaag tttcttccat gcgacgttgt tacgtaaata
    12061 aaccggttcg gcatgttcca ccgccaccgt tttaccctcg gcaaacatct gacacgcaat
    12121 cggcagcata tcttccgccg ctggcagtaa aacttctccg tcgcgcaaaa ccagcccgct
    12181 ctctttaccg agatccggcc aggcttgcca gcccgtccct accgttaccc attcgccgga
    12241 aagctgttgc attcgttcat gaacgagttc gggtttgagt acggcttcgg tttcttcacc
    12301 gcgccagata ccgttttcat cacgctgata ttcggcccag taaacttcgc ccattcgcgc
    12361 gtcaatggct gacagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccatcgt
    12421 cattagcgtg gagacgccaa tcatcggtaa ttccgcgcca agcgccagcc cttgcgcgat
    12481 gccaatacca atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt
    12541 tatgtcagtc agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg
    12601 ttgagtatgt tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag
    12661 ggcgacagag caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc
    12721 ttttttcaat aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg
    12781 tatcgcaagg aaacgaaccg cacgggcaat gtcacgagtg cgtggcgcgg gcggcagact
    12841 ggcgagaaac gtcgcgccgt aaggacgcat caccagccga ttgtcacaaa tcaccagcac
    12901 gccacgatcg tcggcgtcgc gaatcagtcg ccctaccccc tgtttgagag taatgacggc
    12961 atctggtagt tgcacttcat cgaacgggtc gccaccgcgc aaacgacaat cttccatgcg
    13021 cgcttttaac agtggatcat ccggcgaggt aaacggcaac ttgtcgataa tcaccaatga
    13081 caatgtatcg ccacgcacgt ccaccccttc ccagaaacta ctggttgcca caagaagcgc
    13141 gttaccggcg ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa
    13201 aacgggaagc gtcatggtag cgcggaactg ctcggccaga tcacgcatca tggcgtgcga
    13261 ggtacaaagc ataaaacaac gaccgttgtt agcttcgatg atcggtcgca gcattgccgc
    13321 taactggcga gcagaacctg gctggttggt ttgcggcaga ttgcgcggca cacagagtaa
    13381 cgcctggcgg ctgtaatcaa acgggcttgg caatagcaac gactccgcct gttctatccc
    13441 aagccgcgag gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtgaagat
    13501 ccagctacca ggcttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt
    13561 gagagccaga gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt
    13621 gatctctttt agccgcttca gccgtgtgcg atacaacgta gcgcgctcaa atgccgcatc
    13681 cagcaaggcg gaacgcccca gtgacagttt cgccacgtca taacaaagtt ccagggtgtc
    13741 atcgagcagt aaaaatgccc gctgaatttg cggattagct aacagctcac gcaggttacc
    13801 gcgataacct ggctcaccga gttgcagacg aaaatcctgc gcactctggg caagacgatc
    13861 agcgcacttt tgtaactgct gggtgtcttt taattcggtg cggtaagcga tggtgatgtc
    13921 ttttgccagg tcgagcagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat
    13981 gtccggtagc tggtgggctt cgtcgaagat catgacgtcc gcttccggga tcagttcgcc
    14041 aaatccactc tctttaacca ccatatccgc cagaaagaga tgatggttta ccaccaccac
    14101 atcggcgtcc atcgcttttt tacgtgcttt gaccacaaag caatctttat acatcgggca
    14161 gtcgctgcca agacagttgt cgttggtgct ggtgaccagc ggccacgcct gtgaatcttc
    14221 cgccacgctg acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag
    14281 caggatcaca tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc
    14341 gagacgttcg aggcagaggt agtttgagcg ccctttcagc agcgccacgt tgcccgtata
    14401 tttcaatgcc tttgagactg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt
    14461 tgagccggtc gagataatga ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta
    14521 ggttttgccc gtaccggttc ctgcttccac caccagcggc tggccttttt ctatcgcctg
    14581 ggtgacggct accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt
    14641 cgccagctga ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacag
    14701 ggattatgtc aggatgaggg cgctttcgcc agttgaagtg gtgacggcga cctcacattg
    14761 tggcagtctt tgcagcacga aaatggaagg ataatgagga caaaatgatg actatcgttc
    14821 gtatcgatgc tgaagcccgc tggtctgatg tggtaatcca caacaacacg ctctactaca
    14881 ctggtgtacc ggaaaacctc gacgccgatg cctttgagca aaccgccaac acgctggcgc
    14941 agattgatgc cgtgctggaa aaacagggca gcaataaatc gagcattctg gatgccacca
    15001 ttttcctggc cgataaaaac gacttcgcgg cgatgaataa agcgtgggat gcctgggttg
    15061 tcgcgggtca tgcgccggtg cgctgcacgg tacaagcggg tttgatgaac ccgaagtata
    15121 aagttgaaat taagattgtg gctgcggtgt aagctttatc gaagcaaaat aagtcagacg
    15181 ataatttatc gataatactg gtcggtttta cataaatcga ccagagatta agatattacc
    15241 tgttgatgct cctgctaatt cattatttat ttttataatt tcaattttat ctataaaact
    15301 aactatataa ataaccataa ccatgccgtt gtgttcgccc tctgactagc aactctggtt
    15361 ttctttctta gctgaataat ctggttatag cgtgaggact gaccgttaaa tttctccatc
    15421 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
    15481 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagtct
    15541 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
    15601 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
    15661 agcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat
    15721 tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg
    15781 ggcaaaaaaa atggagtctg gtgaacgcac tgtaattaaa aatcctgaat acttttcaac
    15841 atatatgcag gaagaactaa aagcactgg
//
LOCUS       DAFAVF010000096        15734 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFAVF010000096 DAFAVF010000000
VERSION     DAFAVF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.096"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(140..670)
                     /gene="cybB"
                     /locus_tag="LGK42_002991"
     CDS             complement(140..670)
                     /gene="cybB"
                     /locus_tag="LGK42_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBK2659217.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            859..1860
                     /gene="gap"
                     /locus_tag="LGK42_002992"
     CDS             859..1860
                     /gene="gap"
                     /locus_tag="LGK42_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK2659218.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1902..3341)
                     /gene="aldA"
                     /locus_tag="LGK42_002993"
     CDS             complement(1902..3341)
                     /gene="aldA"
                     /locus_tag="LGK42_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBK2659219.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPTNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3538..4338)
                     /locus_tag="LGK42_002994"
     CDS             complement(3538..4338)
                     /locus_tag="LGK42_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK2659220.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4610..8512)
                     /gene="hrpA"
                     /locus_tag="LGK42_002995"
     CDS             complement(4610..8512)
                     /gene="hrpA"
                     /locus_tag="LGK42_002995"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBK2659221.1"
                     /translation="MTEQQKLTFTALQQRLDSLMPRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGVKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            8713..9318
                     /gene="azoR"
                     /locus_tag="LGK42_002996"
     CDS             8713..9318
                     /gene="azoR"
                     /locus_tag="LGK42_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK2659222.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(9369..10685)
                     /locus_tag="LGK42_002997"
     CDS             complement(9369..10685)
                     /locus_tag="LGK42_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBK2659223.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(10675..12432)
                     /locus_tag="LGK42_002998"
     CDS             complement(10675..12432)
                     /locus_tag="LGK42_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBK2659224.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(12448..13344)
                     /locus_tag="LGK42_002999"
     CDS             complement(12448..13344)
                     /locus_tag="LGK42_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK2659225.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLISATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMFPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            complement(13344..13949)
                     /locus_tag="LGK42_003000"
     CDS             complement(13344..13949)
                     /locus_tag="LGK42_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK2659226.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNALLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVSIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(14120..>15734)
                     /locus_tag="LGK42_003001"
     CDS             complement(14120..>15734)
                     /locus_tag="LGK42_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HBK2659227.1"
                     /translation="QMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIM
                     RTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDR
                     EPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPE
                     SIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLTFKLAEMKHHGQLLR
                     MTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQV
                     IHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYET
                     SADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTP
                     RNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNL
                     TFFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNP
                     ELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         4132 a         4137 c         3615 g         3850 t
ORIGIN      
        1 aagccagatg gacgtatggg caaggattat ggttttattt gtcatacaaa taaatataat
       61 aggcgcttcc gatgtagacc cgtattcttc gcctgtacca cgggtcggtt ttagtacagg
      121 cgttttcttg aaatatcctt caggaacgtt tacgcggcat catgcgtaga agtgtgttgt
      181 ccttccagaa atagtggtgt gccagtgcag ccgcagcgtg caacccgatc acaaaatatc
      241 ccagattcgc cagcgtctca tgccacgact ttaagctatc aacccgttcg aaattggcct
      301 ctgaagcgta aggcatcgtc aaaccaaacg caaaccacgg gttgccccgg ttatacatca
      361 tcaccaaacc aatcactggc agcgcaataa acaggagata aatcaccaaa tgtcccaaat
      421 gcgccagtcc cgtcatcatc ggttttggct taggtataat cggcggggtt gggtatttca
      481 gtcttaacaa aagacgaacc accatcagca cgagaattga gataccacag gaaacatgaa
      541 ccatgttgat gagtggccga tcgctacgtg ggaagaaacc acgaaactcc attgcgcaat
      601 acgctgcgat aaccagcaaa aagaccagcc agtgaatgct gatttgtaac cttgagtatt
      661 tattttccat aacatttcct gctttaacat aattttccgt taacataacg ggcttttctc
      721 aaaatttcat taaatattgt tcacccgttt tcagtacgtt acgtttcact gtaagaatgg
      781 agattgccca tcccacaatc ctggtctaag cctggaaagg atcaattttc atccgaatgt
      841 tcctgacagg agaaaaccat gagtaaagtt ggtattaacg gttttggtcg tatcggtcga
      901 ctggtgttgc gtcgattact tgaagtcaaa agcaacatag acgttgtcgc tattaatgat
      961 ctcacttccc caaaaattct cgcctacctg ctgaaacatg attcaaacta cggaccattc
     1021 ccctggagcg ttgattttac ggaagattca cttatcgttg atgggaaaag tatcgcggtt
     1081 tacgccgaaa aagaggctaa aaatattccg tggaaagcga aaggtgcaga aatcattgtc
     1141 gaatgtactg gcttttatac ctccgccgag aaatcgcagg cgcatcttga tgctggtgcg
     1201 aagaaggtgt tgatttccgc ccctgccggt gaaatgaaaa ctatcgttta taacgtcaat
     1261 gacgacactc tggatggcaa cgacaccatt gtttccgtgg cgtcatgcac aactaactgt
     1321 cttgcgccga tggccaaagc cttgcatgac agtttcggga tagaagtcgg cacgatgacg
     1381 accattcatg cctatactgg cacccagtca ctggtggatg gcccacgtgg taaagattta
     1441 cgtgcttcac gcgcagcggc agaaaatatc attccccaca ctacgggggc ggcaaaagcc
     1501 attggtctgg tgatcccgga actgagcggc aaactgaaag gtcatgcgca acgcgtgccg
     1561 gtgaaaacag gttcggtcac tgaactggta tcgattctcg gaaaaaaagt gactgccgaa
     1621 gaggtgaata acgcacttaa acaagcaacc accaataacg agtcatttgg ttataccgat
     1681 gaagaaatag tctcttccga tatcattggc agccatttcg gttcggtgtt tgatgccacg
     1741 caaacggaaa ttaccgccgt gggcgattta caactggtga aaacggtcgc ctggtacgat
     1801 aacgaatatg gcttcgtcac gcagcttatt cgcaccctcg aaaaattcgc taaactctga
     1861 cgcgcacagg cggaggaaaa aacctccgcc tcttttactc attacgactg taaataaacc
     1921 acctgggtct gcagatattc atgcaggcca tgtttaccat ctgcgccgcc aataccggat
     1981 ttacgccatc cggcgtggaa gccttgcata gcttcgaagt tttcacggtt gatgtaagtt
     2041 tcaccaaact tcagcccttt aatggctttc atcgcgacgt tcagattttg ggtatagatt
     2101 gatgaggtca ggccgtaatc actgtcatta gccattgaga tagcctcttc cagcgtgtca
     2161 aatgcgacca ccggcagaac cgggccaaag gtttcctcat gcataatcga catttcctgg
     2221 cgaacatcca gcagcaatgt cggcggataa taatatcctt tcccctctac cgctttgcca
     2281 cccaacgcca ctctcgcccc ttcttctact gcgcgcgcca ctttttgctc aaccctttcc
     2341 agcgccgcgg cgttaatcaa cggccccatc gcaatgtcgt tgcgttcagc ggggttacca
     2401 aattgaaccg cctgcatcgc ttcacccagc cgattgacga actgatcata aatgcctttc
     2461 tgtacataaa tacgttctgc acagttacac acttgcccac tattaatgac gcgtgaatca
     2521 acgatggctt tgactgccag ttcaagatcg gcatcgtcca ttacgatagc tggtgcttta
     2581 ccccccaatt ccagacacac tttggtgatg tttttcgccg cagtcgccat gatcttctca
     2641 cctgcagaga cgctgcctgt catactgacc attgcgacct ttgggttacc cgccagttct
     2701 tgcccaacgg tttcaccacg ccccagtaca aggttaaaca cgccgcgcgg aaggcctatt
     2761 tcatcgacga ttttggcgaa tgcaatcgca ttggttggcg taaattcact aggtttaatg
     2821 acgatggtat taccggtcaa aagagcggga gccattttgc gggcaatgag gaagaacggg
     2881 aagttccacg gcagaatgcc ggtagtcaca ccaagcgcac gtttaaacaa aagaatattt
     2941 tctcctggac gatcgctttg aataatctcg ccctcgtaac gccgtgccca ctccgccatg
     3001 taatcgatat agtcggcagt aaaagccact tcgacttcag ccagctgctg gatcttgccc
     3061 ccttcttcaa caatcagcgc actgatttca ctggcgcgtt cgcggatccc ggcggagatt
     3121 ttgcgcaacc aactggcgcg ttcaatagca ggcaacgctt cccattctgg ttgtgcacgt
     3181 tctgctgcat cgattgcctt acgggcatcc tcggcctgac catcgggtat gcgggaaatg
     3241 acagcctctg tagcagggtt taccacatca atccatgcgt ctccacgcca ggtaacaaac
     3301 tgtccatcga tatacatagg atgttgaacg ggtactgaca tgggcgactc ctgtgattta
     3361 tatgtttgtt ttctgtgaat gcattgttaa ttaacatcaa tcatcagaga gacggcatcg
     3421 tgaaaggaca attctgtgaa gggcttcata aaaacaagtg taaaaggaat aaaaggcaga
     3481 aataaaagtc aggagtcatg cccgtttgca aacaagtggt tcacaaacgg gcaattttta
     3541 acgtaacgag cgactttcca tcgcctcgat gagcaccgca tcatgtttca gcgtttgcca
     3601 tgcatggatg acttctgccg gaaaatcaac gtgaacgata aaatctcgcc cgcggggacc
     3661 gtagccatcg ctatcatcgc gtaaacgcgg cagctcgcca gtgagtagtg agagataacg
     3721 ctcaaccggc cataatcctt gtaactgatt aataaagatt acactgtctg cgttatttcc
     3781 taactgagga acgaatccgg gataacttaa ccagcgtggt gcatcgggat tgtccccagt
     3841 catacggcgg aacgtcgcca tcgtgcgccg ctgcatggtg gggtcctgaa caacgatagc
     3901 cgtatgaact cgttctacgg cctgtttcaa tagcgcgatg ctaaagcgtg cgttttcacc
     3961 gcagtttgtt gactggtctt caatccagat tttttcatgc ggaatgtgcc agaactgatg
     4021 agcgatatcc gccaggatgg tcgcttctgc tctgccagtg gtgcggatag tgttgtagtg
     4081 cggatgctgt gcgatggcgc tatacaaaaa agttgtcgag tgaccgatac caccactaat
     4141 cagtaaagga atttgctgat cgcgagcaat cttacatgcc gcgtcgatag tcggcataac
     4201 cgcattgcct gcaaggatca cgcaatcggc ctgatacggc acctcaccgg agaaatcatc
     4261 ctgcgccagc cactgtccga taacatttat ggcatctata gttgccggcg aaagcgtcgg
     4321 aaacggggta atgttcatgg ttcctcctta tcgttccctt aagaatactg cgtgagataa
     4381 tagtgagaag cggagcactc tgaaaggagg aagtcgtatg tccttaaccg ccactccgca
     4441 agcctgacat atcttttaag gaaatgctgt agataaacat tattcatctt gcagcagaga
     4501 attgtatttt tctgaattcc tgaattttta cccttaccgt tttagaatta ataaaaaacc
     4561 cggcgctaaa tgcttaccgg gttttttctt tatcaggcaa atagcagggt taaccgctaa
     4621 tctgctccat cgcctgcaaa atacgcttat ctgaaatcgg ataaggcgta ccaagttgtt
     4681 gagcgaagta actaacgcgc aactcttcta tcatccaacg gatctctttc acgtcttcat
     4741 cctcacgacg tgcaggcggc agtttgttga tccattgctg ccacgcctgc tggacgtttt
     4801 cgactttcag catctgagca cggtcgcgat gtggatcaac cgccagtttt tccagccgtt
     4861 tttcaatcgc ctgcaaatat cgcagcgtgt cgcccagccg tttgaagccg ttaccagtga
     4921 caaatccgcg atataccaac ccgcccatct gcgctttaat gtcagaaagc cccagtgcca
     4981 tggtcatatc cacccgccct ttcagacgtt tgttgatatt gaacactgcc gtaagaattt
     5041 gctcgacctg cttcgcaata tctaccaccg tgtcgttcag ttcggcacgc actttctcat
     5101 gcagcgcagc aaagccttct tccgtccaga ccgggccacc attggcgtcg atcagttgat
     5161 ccacaccgca ggagatacag tcgtcgatca gctccagcac tttgccatac gggttaaagt
     5221 acagtccaag cttggctttg tttggtaact tctcatgtaa atacttgatc ggcgagggaa
     5281 tatttagcag cagtagacgg cgaagaccgt tccacattgc ctgtttttgc tccagcgggt
     5341 tatcaaacag tttgatcgcc acactgtcac gttcatccac cagcgccggc cacgccttca
     5401 ctttatagtt gccgcgcttc tgttcgtagc tttccggcag ttgcccaaag ctccagatat
     5461 gcagcccgct ctgctcgata ccgtcatccg ccaccgcgga cagcgtttcc tgcactttgc
     5521 ctttcagcgc atctttcaga tcctgtaatg agcgcccttc ttttagcttc ttatttttgt
     5581 cgtccaccac gcggaaggtg attttcaggt gatcgggcac ctgatcccag tgccagtctt
     5641 cgcggtcaac ggtaacaccg gtcatccgcc gtaactcgcg ctcaaggctg tcgagcaacg
     5701 gtaactccag cggtgtgacg cggcctaaaa acgcttcggc gtagtttggt gcgggtacaa
     5761 aattacggcg taccggtttc ggcaacgatt taatcagagc aatcaccagt tcgcggcgca
     5821 gaccggggat ctgccattca aacccgcttt cctcaacctg gttaagtaac ggcagcggaa
     5881 tatgtacggt cacaccgtca gcatccgcgc cgggctcaaa ctgatagctc aaacgcagct
     5941 tgagattgcc ctgatgccag aagttcgggt aatccagctt gctgattttt tctgcgccct
     6001 ctttgatcaa catgcttttt tcaaagttga gcaaatcagg ggtttcgcgg ctgacttttt
     6061 tccaccagct gtcgaagtgg cgtgcagaga tcacatcgtg gctgatgcgc tggtcgtaga
     6121 actcaaacaa cgtttcgtca tcaaccagaa tatcgcggcg acgtgatttg tgttccagct
     6181 cttccacttc ggcccgcagt ttcaggtttt cacggaagaa tgcgtgacgc gtctgccagt
     6241 caccttccac cagcgcatgg cgaataaaga gttcacggca taacgccgga tcgatctggc
     6301 tgtagttgac cttgcgcgcg gcaacaatcg gcaaaccata aacagtgact ttttccgttg
     6361 ccatcaccgc gccctgcgcc cgttcccagt gaggttcgct gtaggtgcgt ttaatcaaat
     6421 gctgagcgac aggctccacc cattccgggt cgatacgcgc agcaatgcgc ccccacaggc
     6481 ggctggtttc taccagttcc gccaccatta cccatttcgg cggttttttg aataaaccag
     6541 aaccgggaaa gatggagaaa cgcgcgttac gtgcgccggt atattcttgt ttatcggcat
     6601 ctttcatgcc gatatgggaa agtaaacccg tcagcaacgc aatgtgaatt tcgcgatact
     6661 ccgccggttc gctgttaacc ggaatgccaa gttctttcac cacctggcgc aactgggtgt
     6721 agatatcctg ccattcgcgc acgcgcagat agttgagata atcggtacga cacaggcgac
     6781 ggaaggcgtt ggaagaaagc gccttttgct gctcgccaag ataattccac agattcacaa
     6841 acgcgaggaa gtcagactct ttgtcgtgga agcgacgatg tttttcgtcc gatgcctgct
     6901 gtttatccat cggacgctcg cgcggatcct gaatggaaag cgcggacgtg ataatcatcg
     6961 cctcacgcac gcagccatgt ttttgcgctt ccagcaccat acgcgccaga cgtggatcga
     7021 caggcaactg cgagagctgg cgaccgagcg gcgtcagttt ataggcgctg gcctgttcat
     7081 ccgtggtgat cgcgcccagt tcttcgagca gacgcacgcc atcctggata ttgcgtttat
     7141 ccggtgcttc gacaaacggg aacgcagcga tatcgcccag ccccagcgcg gtcatctgca
     7201 aaataaccga ggccaggttg gtacgcagaa tctccggatc ggtaaactcc gggcgcgaaa
     7261 ggaaatcgtc ttcggaatag agacgaatac agatcccttc ggacacacgc ccacagcggc
     7321 ctttacgctg attggcagac gcctgcgaaa tgggttcaat cggcaaacgc tgcactttgg
     7381 tgcgatagct gtagcggctg atacgtgctg taccggggtc gataacgtat ttaatccccg
     7441 gtacggtcag cgaggtttcc gcgacgttgg tcgccagcac aatgcgccgt ccgctgtgcg
     7501 actggaacac gcggttctgt tcgctgttcg aaagccgcgc ataaagcggc aagatttcgg
     7561 tatgacgcag atttagcttg ttcagcgcat cggcggtatc gcgaatttcc cgctcaccac
     7621 tcataaagat cagaatgtcg ccagggcttt cctgactcag ttcgtctacg gcgtcaaaaa
     7681 tcgcctgcaa ctgatcgcgc tcggtgtcgt cggcttcttc aacaatcggt cgatagcgca
     7741 cttccaccgg ataggtccga ccggagactt cgataatcgg cgcattatta aagtggcgcg
     7801 aaaagcgttc cgggtcgata gtcgcggaag tgataatgat ttttaggtca ggacgccgcg
     7861 gcagcaactc tttcaaatag ccgagcagaa aatcgatatt caggctgcgt tcgtgtgctt
     7921 cgtcaataat gatggtgtcg tactgcatca gcaggcggtc ttgctggatc tccgccagca
     7981 ggataccgtc ggtcatcagc ttgaccatcg tgttatcact aacgtgatcg ctgaaacgca
     8041 ctttgtaacc gatgcaaccg cccggctccg ttttcagctc ttccgcaata cggttcgcca
     8101 ccgttcttgc cgccaggcga cgcggctggg tatggccgat cagcccttta accccgcgcc
     8161 ccagctccat acagattttc ggtaactgag tcgttttacc agaacccgtt tccccggcga
     8221 cgatcaccac ctggtgatca cgaatcgctt cgagaatgtc ctgttttttc tgactaaccg
     8281 gtaaattgtc aggataagta atttccggtc gtgccgcttc acgcagcagg actttccctg
     8341 ccgcctggtc aatctctttc gccatctcct ggaaaatggc ctgttgtgca tcaggatttt
     8401 taaccttctt aacaccgtgc agacgccgag aaaaacgcag tctgtcgcgc ggcatcagcg
     8461 aatccagccg ctgctgcaag gccgtaaagg tcaatttttg ttgttctgtc ataacgttag
     8521 tgggcagtag tctgcctgat taattttctt ttaaatgata gatatagagt accacatcgc
     8581 ggctttctat gcgttgttca ataaattcga acataggctt cgatttattg cgctatctct
     8641 gtggcaggat tgtgaataaa gtgtcagcaa gcaacggggc atcgcccatt caaacatcta
     8701 taaggaaaca ccatgagcaa ggtattagtt cttaaatcca gcatcctggc agggtactct
     8761 cagtctaatc agttgtccga ttattttgtt gaacaatggc gcgaaaagca ctccgctgat
     8821 gaaatcaccg ttcgcgacct ggctgcaaat ccgattccgg tactggatgg cgaactggtt
     8881 ggtgcactgc gtccgagcga tgcgccgctg actccgcgtc agcaggaagc tctggcgctt
     8941 tccgatgagc tgattgccga gctgaaagcc cacgacgtta tcgttattgc ggcaccgatg
     9001 tataacttca acatctcaac tcagttgaaa aactattttg acctggttgc acgcgcaggc
     9061 gttactttcc gctataccga gaacggtccg gaaggtctgg taacgggtaa aaaagccatc
     9121 gttattacca gccgcggtgg gatccacaaa gatggaccaa cggacctggt gacgccgtat
     9181 ctgtccacgt tcctcggctt tatcggcatt accgatgtga aatttgtctt cgccgaaggg
     9241 atcgcatacg gtccggaaat ggcagcgaaa gcgcagtctg acgcgaaagc ggccatcgac
     9301 agcattgttg ctgcataacc ggacacgatc ttgatgtgga ctgtagttca gtccacatct
     9361 caatccactt acctgttttc ccataatttc agcatcgctt tgtgatcttc cttaagcaca
     9421 atatgcgaac gtctggctcg aatataacta atcgcttcat caacggtttt acagtgtccg
     9481 taacataaca accatgccgc cactaccagc gcactgcgcg ataatcccaa cgcgcaatgg
     9541 accagaacgc tgccttgctc ttcgcgtaat gtttccagca tcgccacggc ctgtcggagc
     9601 tccccctctt ccggaaccac cagatccagc atcggtacac agaaatagag tcgatctttt
     9661 gtcgctcgtc cgcgagggaa ttcaaaggtg acgtccagaa ccgcattctg tgccggaata
     9721 tgtcgtggaa acgcccctaa ataaacacca gcagtcattt tgctcaccgg ctccaggcga
     9781 cgacaaaacc aacgcataga cagccacatc ccgatgcggc agggcaatgt cagccagtaa
     9841 acggcgggcg ggagtttccc ctgactatct ttccctgttg ttatcgcgcc aagcccaccg
     9901 tacccacggc caataatgag tagcgataat actggccaac ataaccagac tgaccaccat
     9961 aactgaatca tcaccattag ctccatcaac acaatgcacg agcccgcgcc tacgacatat
    10021 ggcagtgcta ttttgattcg acgttgatca ggtttctgat aattccaacg acgatcgacg
    10081 ggcaccatcc agtcaatcaa catacctacc gccagccctg tgatgacatc aataaaatga
    10141 tgctgccagg tcgtcagagt agaaatagcg atgagtaaaa accatccgcc acagacttta
    10201 cgccacctct cagccagatg ctgacgaaag tgacgccaaa gtagccagca gagaataata
    10261 tgcagcgaag gagactggtt ataaggcaga tcaaacagtt caagttgcga aaatagccat
    10321 cccgtcaccc cactcacttc aggacggata aaactaaact tcagcggata gagcaaaaaa
    10381 ccgcagcagg ccattaccgt tgccagaata agccggtgga caagtcggcg ctgttcgaat
    10441 gtcgtgctac aaacgaacag cgaaaatcca tataaaagat ccagactcca gtaaggaaca
    10501 atagtccagg gaagaaaagg gatcgccgtt tcccaaccga atacctgact ggggatatca
    10561 tggctgttaa ggtcctgaac cgcggtgaac tgattaagag atccataggt gaagaagaaa
    10621 aacggggcca gcaacaataa ccagccagcg ccttgtagca atacgttccg acgttcagtt
    10681 atcacgacgc accgccatcg aaaccgtaaa aataccccac tcatcaatcc gttgtgtgca
    10741 tttatcaaat ccggcatcac gcacgagtga atccatctcc ccttgcgaac gcacgcgcat
    10801 cacccacggt ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg
    10861 gtgccacggc tgcccggtgt agatgagaat gccacccggt tcgatggcat tggcaagacc
    10921 tgcgagggag ttttttacct gctcattttc gggaaaaagc tcatacaggc cagagacaat
    10981 cgccagcgta ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg
    11041 ttcaaaacgc acctgcccag acattccccg ttgggcaatc atctcttgcc cctgtgcaac
    11101 atttaactca ctgtaatcac gtaacaaaat atcgcatacg gcaggttcat tctccagcgc
    11161 atccagtaca tagcgcccat gccctgcggc aatgtcaacc acgcggatgg ctaaaccttt
    11221 ggcgtggaga tcggcaacgg cttgtttaat cagcatttgt aaatgggttt tgcgctggcg
    11281 aataccgcgc cagccaacac tgttcaggta gattttgtcg actaaacgcc cgaatgcgtt
    11341 actaccttgt ggttgatttt gatagacata atccagcgaa ctgcctgaat caaagccggt
    11401 gctcattccg agatgcaggc ccgcagagtg cgccccgaac aatttcatcg ccatacgcat
    11461 aaagcgatac gccaaatcaa ccggcgataa tggcacgggt ccaccagaaa gtagccgcca
    11521 tcgatccgct gatggtcctg tgcagtcttc atgctgataa tcaaattttt gcgatttgtt
    11581 agcatataaa tgactaataa agctttgcat tttttcaaat gccagcgccc tgttctcttc
    11641 acccaacgtg tcgtgataaa agcctggcag cagatgcagc tctttgagag ggctacgtaa
    11701 acgctgataa aaatcgatct gcggttggcg atgcaccaca tagtcatcgc ctgatatcag
    11761 aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca atacgttcag acgttttata
    11821 gagatcgagc aagatattga cggcaatcgc ccgtgtgatc agcggatcat tattgaaact
    11881 cgccacccgt tgccgatcgt gggtcaaata gcgtcctttc acataggaat taataaaaaa
    11941 cagaccacgc agacgatgcc ataacgccag cgcaggacgt gccagcggca catacaattt
    12001 aaccttaaag gccggagaag ccagcaccag cccgcgaatt gcaggcgcat aatcatgaac
    12061 ccatgtggca accagcactg cgccgacgct ttgcgcgatc acaaccacct cttccagtcc
    12121 aacctggctg tcgctggcag caaaacggac aaattcatcg acatcctgca ctgaacgcgc
    12181 aagagatggg ctataaccac gcggccccga agtttttcca tgcccccggg catcccatgc
    12241 ataaaaagca gtatctggca tcgccagttc atcaacgaga tgttgtagac gaccagaatg
    12301 ttcatgaccc cgatgaaata agacgatgac ctttttcgcc ccgggctgta aagcgggcca
    12361 gtgccgataa aacaacgctg tattatcact ggtggtaaaa aaatgttccc cagggatgcg
    12421 tgaattttcc attttctcta ttttccttca gtaacagctg tagcgaataa aataaaaaaa
    12481 taccggagcg gtgaaaatta atgagtcaat cctgtcaagg agtccgccat gtcctggtaa
    12541 tagttttcca ctatctttaa caccaatatc tcgtttgatg gctgacatca cgacatcgcc
    12601 gcaaaaacca ctaataccaa ttaacaaacc cgctaataat gcctgtaatg tatttagcgg
    12661 tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg gtgatgacgc cccccaccag
    12721 accctccagc gtttttcccg ggctgacttt ggggaccact tttcttctgc cgcaggattt
    12781 tccccataaa tactgtgcaa tatcgtttga ctcggttaat gccaaaagaa ataacaccag
    12841 taacgccccg ccctggatat tcatggatgg aaacattaat aaccaggcgg catgactcaa
    12901 ggcgaaaacg gtcattatcc agccccaaaa aatagcgctc acggtatgca gaaaaccgga
    12961 gggttcaccc acaaaaactc gccaggtggc tgatatcaga aacccagaca aaggtataaa
    13021 taacagaaac agctcaaagc aattaaaacc gataagcaaa tagttaagag gaataaccca
    13081 atataaccac cgcggaaaat gtatggatat aagcgtacaa tattctttca atgccagaaa
    13141 acttatcagc gcaaaaaacg tcaacgtcat ccagcgtgga cctgaaatca ccagtgaaaa
    13201 acaaatgacg atgccccacc aggtacgaat acgtaatgta acctcacccc cttttctctc
    13261 cggtaaccgc catagcagaa aacggtttat cagcgttgct aaaattaaca acgcgaaaag
    13321 tgttgccaga gatttttcca gcattatctt tcagcactca taaaaagcac actccgacaa
    13381 cgattaatcg cagtccagag aagcagaatt gaagcaatgc tccataaaag attattccat
    13441 tgcatccact gtggataaat ggaaacggcc agcccccaca acccaaatat taacgcacga
    13501 tcgcttttgc cgaatggacc ggcataactg cgaataccgt taattgtctg cgcgagtaaa
    13561 ccacaaaact cagtcaatat ggtgcaaaat aacatgagta ttacgagtaa tgcgttactt
    13621 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtttca
    13681 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
    13741 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
    13801 gccaccaaca ccaacagcaa tccggtaaga agagaaagcg ccagtgcagc aagagtgatg
    13861 tgattcgctg taacgtggtg cttgtaaagc caaaacatcg tcggccttaa cagcgactga
    13921 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
    13981 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
    14041 atatcaaggt attggggact cactgtcagg aataattatc cccaattaat caaagggtta
    14101 agaaatatta cgttgtggat caatacaact catcaagttt tctcatcgct tgttcccgta
    14161 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
    14221 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt taatacatca actggaaaac
    14281 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtactgcag
    14341 gcggtgtgtg gcggttgtta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
    14401 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
    14461 cggggtcgag tgcataatag acgcgtaatg cttcatcaac ctgcgcacgg gagctgtttt
    14521 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtattttgc tgtaattcct
    14581 tgaggacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
    14641 cttccaaacg tatcagggcg gtggcattta atagcatctg gtcatcaact tcaccgtggc
    14701 tgataagctc agacaaaatc atatcagcgc tggtttcata cgcagctgaa tctgctttcg
    14761 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
    14821 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
    14881 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
    14941 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
    15001 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
    15061 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
    15121 acgttaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
    15181 aggtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
    15241 catcaataat ggattcaggc aataaccacg catctttata aagctcctga atgatcatat
    15301 cccaatctct tttcgccaga gtcattacga tatccagagg gagattttca cgtagcgcaa
    15361 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
    15421 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
    15481 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
    15541 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
    15601 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
    15661 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
    15721 cttttttcat ctga
//
LOCUS       DAFAVF010000097        15645 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFAVF010000097 DAFAVF010000000
VERSION     DAFAVF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.097"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            338..742
                     /gene="sdhC"
                     /locus_tag="LGK42_003002"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             338..742
                     /gene="sdhC"
                     /locus_tag="LGK42_003002"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBK2659228.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            736..1083
                     /gene="sdhD"
                     /locus_tag="LGK42_003003"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             736..1083
                     /gene="sdhD"
                     /locus_tag="LGK42_003003"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBK2659229.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1083..2849
                     /gene="sdhA"
                     /locus_tag="LGK42_003004"
     CDS             1083..2849
                     /gene="sdhA"
                     /locus_tag="LGK42_003004"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBK2659230.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2865..3581
                     /gene="sdhB"
                     /locus_tag="LGK42_003005"
     CDS             2865..3581
                     /gene="sdhB"
                     /locus_tag="LGK42_003005"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBK2659231.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3782..6583
                     /gene="sucA"
                     /locus_tag="LGK42_003006"
     CDS             3782..6583
                     /gene="sucA"
                     /locus_tag="LGK42_003006"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBK2659232.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6598..7815
                     /gene="odhB"
                     /locus_tag="LGK42_003007"
     CDS             6598..7815
                     /gene="odhB"
                     /locus_tag="LGK42_003007"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBK2659233.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7909..9075
                     /gene="sucC"
                     /locus_tag="LGK42_003008"
     CDS             7909..9075
                     /gene="sucC"
                     /locus_tag="LGK42_003008"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBK2659234.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9075..9944
                     /gene="sucD"
                     /locus_tag="LGK42_003009"
     CDS             9075..9944
                     /gene="sucD"
                     /locus_tag="LGK42_003009"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBK2659235.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10048..10770)
                     /gene="mngR"
                     /locus_tag="LGK42_003010"
     CDS             complement(10048..10770)
                     /gene="mngR"
                     /locus_tag="LGK42_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBK2659236.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQAKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10939..12855
                     /gene="mngA"
                     /locus_tag="LGK42_003011"
     CDS             10939..12855
                     /gene="mngA"
                     /locus_tag="LGK42_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBK2659237.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12873..15506
                     /gene="mngB"
                     /locus_tag="LGK42_003012"
     CDS             12873..15506
                     /gene="mngB"
                     /locus_tag="LGK42_003012"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBK2659238.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         3743 a         3926 c         4317 g         3659 t
ORIGIN      
        1 attgtcaaat tagatgatta aaaattaaat aaatgttgtt atcgtgacct ggatcactgt
       61 tcaggataaa acccgacaaa ctatatgtag gttaattgta atgattttgt gaacagccta
      121 tactgccgcc aggtctccgg aacaccctgc aatcccgagc cacccagcgt tgtaacgtgt
      181 cgttttcgca tctggaagca gtgttttgca tgacgcgcag ttatagaaag gacgctgtct
      241 gacccgcaag cagaccggag gaaggaaatc ccgacgtctc caggtaacag aaagttaacc
      301 tctgtgcccg tagtccccag ggaataataa gaacagcatg tgggcgttat tcatgataag
      361 aaatgtgaaa aaacaaagac ctgttaatct ggacctacag accatccggt tccccatcac
      421 ggcgatagcg tccattctcc atcgcgtttc cggtgtgatc acctttgttg cagtgggcat
      481 cctgctgtgg cttctgggta ccagcctctc ttcccctgaa ggtttcgagc aagcttccgc
      541 gattatgggc agcttcttcg tcaaatttat catgtggggc atccttaccg ctctggcgta
      601 tcacgtcgtc gtaggtattc gccacatgat gatggatttt ggctatctgg aagaaacatt
      661 cgaagcgggt aaacgctccg ccaaaatctc ctttgttatt actgtcgtgc tttcacttct
      721 cgcaggagtc ctcgtatggt aagcaacgcc tccgcattag gacgcaatgg cgtacatgat
      781 ttcatcctcg ttcgcgctac cgctatcgtc ctgacgctct acatcattta tatggtcggt
      841 tttttcgcca ccagtggcga actgacatat gaagtctgga tcggtttctt cgcctctgcg
      901 ttcaccaaag tgttcaccct gctggcgctg ttttctatct tgattcatgc ctggatcggc
      961 atgtggcagg tgttgaccga ctacgttaaa ccgctggcat tgcgcctgat gctgcaactg
     1021 gtgattgtcg ttgcactggt ggtttacgtg atttatggat tcgttgtggt gtggggtgtg
     1081 tgatgaaatt gccagtcaga gaatttgatg cagttgtgat tggtgccggt ggcgcaggta
     1141 tgcgcgcggc gctgcaaatt tcccagagcg gccagacctg tgcgctgctc tctaaagtct
     1201 tcccgacccg ttcccatacc gtttctgcgc aaggcggcat taccgttgcg ctgggtaata
     1261 cccatgaaga taactgggaa tggcatatgt acgacaccgt aaaagggtcg gactatatcg
     1321 gtgaccagga cgcgattgaa tatatgtgta aaaccgggcc ggaagcgatt ctggaactcg
     1381 aacacatggg cctgccgttc tcgcgtcttg atgatggtcg tatctatcaa cgtccgtttg
     1441 gcggtcagtc gaaaaacttc ggcggcgagc aggcggcacg cactgcggcg gcggctgacc
     1501 gtaccggtca cgcactgttg cacacgcttt atcagcagaa cctgaaaaac cacaccacca
     1561 ttttctccga gtggtatgcg ctggatctgg tgaaaaacca ggatggcgcg gtggtgggtt
     1621 gtaccgcact gtgcatcgaa accggtgaag tggtttattt caaagcccgc gctaccgtgc
     1681 tggcgactgg cggggcaggg cgtatttatc agtccaccac taacgcccac attaacactg
     1741 gcgacggcgt cggcatggct atccgcgcag gcgtaccggt gcaggatatg gaaatgtggc
     1801 agttccaccc gaccggtatt gccggtgcgg gcgtactggt caccgaaggt tgccgtggtg
     1861 aaggcggtta tctgctgaac aaacatggcg aacgctttat ggagcgttat gcgccgaacg
     1921 ccaaagacct ggcgggccgt gacgtggtgg cgcgttccat catgattgaa atccgtgaag
     1981 gtcgcggctg tgatggtccg tgggggccac atgcgaaact gaaactcgac cacctgggta
     2041 aagaagttct cgaatcccgt ctgccgggta ttctggaact ctcccgtacc ttcgctcacg
     2101 ttgatccggt gaaagagccg attccggtta tcccaacctg tcactacatg atgggtggta
     2161 ttccgaccaa agtgaccggt caggcgctga cggtgaacga gaaaggcgaa gatgtggttg
     2221 ttccggggct gtttgccgtt ggtgaaatcg cttgtgtatc ggtacacggc gctaaccgtc
     2281 tgggcggcaa ctcgctgctg gacctggtgg tctttggtcg cgcagcgggt ctgcatctgc
     2341 aagagtctat cgccgagcag ggcgcattgc gcgatgccag cgagtctgat gttgaagcat
     2401 ctctggatcg tctgaaccgc tggaacaata atcgtaacgg tgaagatccg gtggcgattc
     2461 gtaaagcgct gcaagaatgt atgcagcata acttctcggt cttccgtgaa ggtgatgcga
     2521 tggcgaaagg gcttgagcag ttgaaagtta tccgcgagcg tctgaaaaat gcccgtctgg
     2581 atgacacttc cagcgagttc aacacccagc gcgttgagtg cctggaactg gataacctga
     2641 tggaaacggc gtatgcaacg gctgtttctg ccaacttccg taccgaaagc cgtggcgcgc
     2701 atagccgctt cgacttcccg gatcgtgatg atgaaaactg gctgtgccac tccctgtatc
     2761 tgccagagtc ggaatccatg acgcgccgaa gcgtcaacat ggaaccgaaa ctgcgcccgg
     2821 cattcccgcc gaagattcgt acttactaat gcggagacag gaaaatgaga ctcgagtttt
     2881 caatttatcg ctataacccg gatgttgatg atgctccgcg tatgcaggat tacaccctgg
     2941 aagcggaaga aggtcgcgac atgatgttgc tggatgcgct tatccagctg aaagagaaag
     3001 atcccagcct gtcgttccgc cgctcctgcc gtgaaggtgt gtgcggttcc gacggtctga
     3061 acatgaacgg caagaatggt ctggcctgta ttaccccgat ttcggcactc aaccagccgg
     3121 gcaagaagat tgtgattcgc ccgctgccag gtttaccggt gatccgcgat ttggtggtag
     3181 acatgggaca attctatgcg caatatgaga aaattaagcc ttacctgttg aataatggac
     3241 aaaatccgcc agctcgcgag catttacaga tgccagagca gcgcgaaaaa ctcgacgggc
     3301 tgtatgaatg tattctctgc gcatgttgtt caacctcttg tccgtctttc tggtggaatc
     3361 ccgataagtt tatcggcccg gcaggcttgt tagcggcata tcgtttcctg atcgatagcc
     3421 gtgataccga gactgacagc cgcctcgacg gtttgagcga tgcattcagt gtattccgct
     3481 gtcacagcat catgaactgc gtcagtgtat gtccgaaggg gctgaacccg acgcgcgcca
     3541 tcggccatat caagtcgatg ttgttgcaac gtaatgcgta attaaatgtt gatgccgggg
     3601 aaatcccccg gctattcagg aaatctttaa aaactgcccc tgacactaag acagttttta
     3661 aaggttcctt cgcgagccac tacgtagaca agagctcgca agtgaacccc ggcacgcaca
     3721 taactgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt aagggatcac
     3781 gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg gcgcaaacca
     3841 gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg ttgacgctaa
     3901 ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc aattccactc
     3961 tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact cttcaacgat
     4021 ctccgaccct gacaccaatg taaagcaggt taaagtcctg cagctcatta acgcataccg
     4081 cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc aagataaagt
     4141 ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg agaccttcaa
     4201 cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc tggaagccct
     4261 caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacattacca gcaccgaaga
     4321 aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata gcgaagagaa
     4381 aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc tcggcgcaaa
     4441 attccctggc gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc cgatgcttaa
     4501 agagatgatc cgccacgctg gcaacagcgg cacccgggaa gtggttctcg ggatggcgca
     4561 ccgtggtcgt ctgaacgtgt tggtgaacgt gctgggtaaa aaaccgcaag acttgttcga
     4621 cgagtttgcc ggtaaacata aagaacacct cggcacgggt gacgtgaaat accacatggg
     4681 cttctcgtct gacttccaga ccgatggcgg cctggtgcac ctggcgctgg cgtttaaccc
     4741 gtctcacctt gagattgtaa gcccggtcgt tatcggctct gttcgtgccc gtctggacag
     4801 acttgatgag ccgagcagca acaaagtgct gccaattacc atccacggtg acgccgcagt
     4861 gaccggacag ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg gttatgaagt
     4921 tggcggtacg gtacgtatcg ttatcaacaa ccaggttggt ttcaccacct ctaatccgct
     4981 ggatgcccgt tctacgccgt actgtactga tatcggcaag atggttcagg caccgatttt
     5041 ccacgttaac gcggacgatc cggaagccgt tgcctttgtg acccgtctgg cgctcgattt
     5101 ccgtaacacc tttaaacgtg atgtcttcat cgacctggta tgctaccgcc gtcacggcca
     5161 caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa tcaaaaaaca
     5221 tccgacaccg cgcaaaatct acgctgacaa gctggagcag gaaaaagtgg cgacgctgga
     5281 agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg attgcgtggt
     5341 agcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtacc tcaaccacga
     5401 atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggaac tggctaaacg
     5461 catcagcacg gtgccggaag cggttgaaat gcagtctcgc gttgccaaga tttatggcga
     5521 tcgccaggcg atggctgccg gtgagaaact gttcgactgg ggcggcgcgg aaaacctcgc
     5581 ttacgccacg ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag actccggtcg
     5641 cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt ccacttacac
     5701 gccgctgcaa cacatccata acggccaggg cgcgttccgt gtttgggact ccgtactgtc
     5761 tgaagaagca gtgctggcgt ttgaatacgg ttatgccacc gcagaaccac gcaccctgac
     5821 catctgggaa gcgcagttcg gtgacttcgc caacggtgcg caggtggtta tcgaccagtt
     5881 catctcctct ggcgagcaga aatggggccg gatgtgtggt ctggtgatgt tgctgccgca
     5941 cggttacgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt atctgcaact
     6001 ttgtgctgag caaaacatgc aggtttgcgt accgtctacc ccggcacagg tttaccacat
     6061 gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt cgccgaaatc
     6121 cctgctgcgt catccgctgg cggtttccag cctcgaagaa ctggcgaacg gcaccttcct
     6181 gccagccatc ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg tagtaatgtg
     6241 ttctggtaag gtttattacg acctgctgga acagcgtcgt aagaacaatc aacacgatgt
     6301 cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc aggaagtgtt
     6361 gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc tcaaccaggg
     6421 cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg cttctctgcg
     6481 ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg ttcaccagaa
     6541 acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg atacacaatg
     6601 agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc caccgtcgca
     6661 acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt agaaatcgaa
     6721 actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga tgcggttctg
     6781 gaagatgaag gtacaacggt aacgtctcgt cagatccttg gtcgcctgcg tgaaggcaac
     6841 agcgccggta aagaaaccag cgccaaatct gaagagaaag cgtccactcc ggcgcaacgc
     6901 cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat ccgtcgcctg
     6961 ctggctgaac acaatctcga cgccagcgcc attaaaggca ccggtgtggg tggtcgtctg
     7021 actcgtgaag atgtggaaaa acatctggcg aaagccccgg cgaaagagtc tgctccggca
     7081 gcggctgatc cggcggcgca accggctctg gctgcacgta gtgaaaaacg tgtcccgatg
     7141 actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc caccgccatg
     7201 ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa gcagtacggt
     7261 gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta tgtccttcta cgtgaaagcg
     7321 gtggttgaag ccctgaaacg ttacccggaa gtgaacgcgt ctatcgacgg cgatgacgtg
     7381 gtttaccaca actatttcga cgtcagcatg gcggtttcta cgccgcgcgg cctggtgaca
     7441 ccggtactgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa aatcaaagag
     7501 ctggcagtca aaggccgtga cggcaagctg accgttgaag atctgaccgg tggtaacttc
     7561 accatcacca acggtggtgt gttcggttcc ctgatgtcta cgccgatcat caacccgccg
     7621 cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc cgatggcggt gaatggtcag
     7681 gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgtct gatcgatggt
     7741 cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc gacgcgtctg
     7801 ctgctggacg tgtagtagtt taagcttcac ctgcactgta gaccgacgtg gatagggtgt
     7861 cggtctacgg tttaaaagat aacgattact gaaggatgga cagaacacat gaacttacat
     7921 gaatatcagg caaaacaact ttttgcccgc tatggcttac cagcaccggt gggttatgcc
     7981 tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg cccgtgggta
     8041 gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa agttgtaaac
     8101 agcaaagaag acatccgtgc ttttgcagaa aactggctcg gcaagcgtct ggtaacgtat
     8161 caaacagatg ccaatggcca accggttaac cagattctgg ttgaagcagc gaccgatatc
     8221 gctaaagagc tgtatctcgg tgccgttgta gaccgtagtt cccgtcgcgt ggtctttatg
     8281 gcctccaccg aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc gcacctgatc
     8341 cataaagttg cgcttgatcc gctgacaggc ccgatgccgt atcagggacg cgagctggcg
     8401 ttcaaactgg gtctggaagg taaactggtt cagcagttca ccaaaatctt catgggcctg
     8461 gcgaccattt tcctggagcg cgacctggcg ttgatcgaaa tcaacccgct ggtcatcacc
     8521 aaacagggcg atctgatttg cctcgacggc aaactgggcg ctgacggcaa cgcactgttc
     8581 cgccagcctg atctgcgcga aatgcgtgac cagtcgcagg aagatccgcg tgaagcacag
     8641 gctgcacagt gggaactgaa ctacgttgcg ctggacggta acatcggttg tatggttaac
     8701 ggcgcaggtc tggcgatggg tacgatggac atcgttaaac tgcacggcgg cgaaccggct
     8761 aacttccttg acgttggcgg cggcgcaacc aaagaacgtg taaccgaagc gttcaaaatc
     8821 atcctctctg acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg tatcgttcgt
     8881 tgcgacctga tcgctgacgg tatcatcggc gcggtagcag aagtgggtgt taacgtaccg
     8941 gtcgtggtac gtctggaagg taacaacgcc gaactcggcg cgaagaaact ggctgacagc
     9001 ggcctgaata ttattgcagc aaaaggtctg acggatgcag ctcagcaggt tgttgccgca
     9061 gtggagggga aataatgtcc attttaattg ataaaaacac caaggttatc tgccagggct
     9121 ttactggtag ccaggggact ttccactcag aacaggccat tgcatacggc actaaaatgg
     9181 ttggcggcgt aaccccaggt aaaggcggta ctacccacct cggcctgccg gtgttcaaca
     9241 ccgtgcgtga agctgttgct gctactggcg ctaccgcttc tgttatctac gtaccagcac
     9301 cgttctgcaa agactccatt ctggaagcta ttgatgcagg catcaaactg attatcacca
     9361 tcactgaagg catcccgacg ctggatatgc tgactgtgaa agtgaaactg gacgaagccg
     9421 gcgttcgtat gatcggcccg aactgcccgg gcgttatcac tccgggtgaa tgcaaaatcg
     9481 gtatccagcc gggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc cgttccggta
     9541 cactgaccta tgaagcggtt aaacagacca cggattacgg tttcggtcag tcgacctgtg
     9601 ttggtatcgg cggtgacccg atccctggct ctaactttat cgacattctc gaaatgttcg
     9661 aaaaagatcc gcagaccgaa gcgatcgtaa tgatcggtga gatcggcggt agcgctgaag
     9721 aagaagcggc agcgtacatc aaagagcatg ttaccaagcc agttgtgggt tacatcgctg
     9781 gtgtgactgc gccgaaaggc aaacgtatgg gccacgcggg tgccatcatt gccggtggga
     9841 aagggactgc ggatgagaaa tttgctgctc tggaagccgc aggcgtgaaa accgttcgca
     9901 gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct gtaataagaa
     9961 atagccctcg ccgcttccct ctacaggaat ggcgaagggc tgtcggtttc gacatggttg
    10021 gccatcgtat gatggccttt tttgtgctta tcgcgatgat tttcgttgcg ctatcagggt
    10081 aaatttatag tcatcggtat taaaagcgtt gcggctatat tcaaacaccc gaccatcaac
    10141 taaatatcca cgcgatactt tttcaagaat cggctttgcc tggctgatat taagcagacg
    10201 gctcatctct tcggttggca tcagaggaat gatttcctgt tcgctacgat cgataaccat
    10261 tttcttcact tcttcaataa agtgatattt tgaattttcc atgacctgcc aggtgagatc
    10321 cgggaacaac gcaagcggca tccaggtttc ttccagcgcc attggctttt gcttgcgata
    10381 gcgcacgcgc ttcacatgcc acacacgatc ctgcgcggtg atttgtagct gttgctgaag
    10441 aaaatcgtca gccggaatca cttcaaatat caggacttca ctgtgtgtat cgacgtgacg
    10501 gtccgacagt ttttcatcaa aactggttaa ctgaaaaata tcgtaattga cccgctcttc
    10561 tttgacgtaa gtcccgctgc cctgaatgct ttcgaggatc tgttgctcga ctaactggcg
    10621 caaagcctga cgcaccgtaa cccggctgac gccaaactct gtttgtaatg ccgattcggt
    10681 aggtaacgca tcgccaggtt taagctcgcc acgcgcaatt tgttcacgaa tgcgatcggc
    10741 aatctgccgg tataagggct tgtgtcccat ttttagtatc tcattaatac gaatttagcc
    10801 attatgcccg ataaattcat cctgtaaata atacaaatac aatacaaata atttcaatca
    10861 agtgaaattg atcacataat ggtattgttt tatcgggcac actggcgcga ctataaaaac
    10921 gatcaagtga ggatcatgat gaatctgacg actctgaccc accgcgatgc gttgtgtctg
    10981 aatgcgcgct ttaccagccg tgaagaggcc atccacgcgt tgactcaacg tcttgctgct
    11041 ctggggaaaa tttccagtac tgagcaattt ctggaagaag tgtatcgccg tgaaagcctt
    11101 ggcccgaccg ccttaggtga agggttggct gtgccgcatg gcaaaactgc tgcggtaaaa
    11161 gaagcggcgt ttgcggtcgc cacactcagc gagccgcttc agtgggaagg cgttgatggc
    11221 ccggaagcgg ttgatttagt ggtgctgctg gcgattcccc ccaatgaagc gggtactacg
    11281 catatgcaac tgctgacagc gctgaccacg cgcttggcgg atgatgagat tcgggcgcgt
    11341 atacagtcgg cgacgacgcc tgatgagttg ctctcggcgc tggatgacaa gggaggcacg
    11401 caaccttctg cctctttttc caacgcgcca actatcgtct gcgtaacggc ctgtccggcg
    11461 ggtattgctc acacctatat ggctgcggaa tatctggaaa aagccggacg caaactcggc
    11521 gtaaatgttt acgttgaaaa acaaggcgct aacggcattg aagggcgttt aacggcggat
    11581 caactcaata gtgcaaccgc ctgtattttt gcggctgaag tcgccatcaa ggagagtgag
    11641 cgttttaatg gcattcccgc gctttcagtg cctgttgccg agccgattcg ccatgcagaa
    11701 gcgttgatcc aacaagcgct taccctcaag cgtagcgatg agacgcgtac cgtacagcaa
    11761 gatacgcaac cggtgaaaag tgtcaaaacg gagctgaaac aggcactgtt gagcggaatc
    11821 tcttttgccg taccgttgat tgtcgcgggg ggcacggtgc tggcggtcgc ggtattactg
    11881 tcgcaaatct tcgggctaca agatctgttt aatgaagaaa actcctggct gtggatgtac
    11941 cgcaagctgg gcggcgggct gctcggaatt ttgatggtac cggtgctcgc ggcctatacc
    12001 gcctattctc tggcagataa accggcgtta gcgccaggct ttgcggctgg acttgccgcc
    12061 aacatgatcg gctccgggtt tctcggcgcg gtcgttggcg gattgatagc cggttacttg
    12121 atgcgctggg tgaaaaatca cttgcgtctt agcagtaaat tcaatggatt cctgactttt
    12181 tatctctacc cggtgctcgg tacgttggga gcgggcagtc tgatgctgtt tgtggtggga
    12241 gaacctgtcg cctggatcaa taactcgctt accgcctggc tgaacggtct gtcaggaagt
    12301 aacgcgctgt tgctgggtgc cattctcggt tttatgtgtt cctttgacct tggagggcca
    12361 gtgaacaaag ccgcttatgc attctgcctg ggcgcaatgg cgaacggcgt ttacggcccg
    12421 tatgccattt tcgcctccgt caaaatggtt tcggcattta ccgtaaccgc ttccacgatg
    12481 ctcgcaccgc gcctgtttaa agagtttgaa attgagaccg ggaaatccac ctggctgtta
    12541 gggctggcag gtattaccga aggggcgatc ccgatggcga ttgaagatcc gctgcgggtt
    12601 attggttcgt ttgtgctggg ctctatggta acgggcgcta ttgtcggtgc gatgaatatc
    12661 ggcctttcga cacccggtgc cggcattttc tcgctctttt tacttcatga taatggcgcg
    12721 ggcggtgtta tggcggcaat tggctggttt ggtgcggcat tggtgggggc tgcaatctcg
    12781 actgcaattc tcctgatctg gcggcgtcac gcggttaagc atggcaacta tctgactgat
    12841 ggcgtaatgc cataaacaaa aaggaaacga cgatgaaagc ggtatctcgc gttcacatca
    12901 ccccgcatat gcattgggat cgggagtggt atttcaccac cgaagagtca cgcattctgc
    12961 tggtcaataa tatggaagag atcctgtgcc gactggaaca ggacaacgaa tacaaatatt
    13021 acgtactcga cgggcaaacg gcgatcctcg aagattattt cgcggtgaaa ccggaaaaca
    13081 aagaccgcgt gaagaaacag gtagaagccg gtaagttgat tatcggcccc tggtataccc
    13141 agaccgatac cacgattgtt tctgcggaat ccatcgtccg taatctgatg tacggaatgc
    13201 gtgactgcct cgcgtttggc gagccgatga aaataggtta tttaccggat tcctttggca
    13261 tgtccgggca actgccgcat atctacaatg gatttggcat tacccgcact atgttctggc
    13321 gcggatgttc ggagcgccac ggtactgata aaaccgagtt tttatggcaa agcagtgacg
    13381 gtagcgaagt gacggcgcag gtgctgccgt tgggttatgc catcggtaag tacttacctg
    13441 ccgacgaaaa cggattacgc aaacgcctcg acagttattt tgacgtgctg gaaaaagcgt
    13501 ctgtaaccaa agagatttta ttgccgaatg gtcatgacca gatgccattg cagcaaaata
    13561 tcttcgaagt gatggagaaa cttcgcgaaa tttatcctca acgaaagttt gtgatgagcc
    13621 gctttgaaga ggtattcgag cagattgaag cgcagcgtga aagtctggca accctgaaag
    13681 gggaatttat tgatggcaaa tatatgcgcg tgcatcgcac catcggttct acgcgtatgg
    13741 atatcaaaat tgcccacgcg cgtattgaaa ataagattgt taatctgctg gaaccgctgg
    13801 caacactggc ctggacgttg ggttttgaat accaccacgg cttgctggag aaaatgtgga
    13861 aagagatctt aaaaaatcat gcccacgaca gtatcggttg ttgttgcagt gacaaagttc
    13921 atcgcgaaat cgtcgcccgc ttcgaactgg ctgaagacat ggcggataat ctgattcgtt
    13981 tctacatgcg caaaattgcc gacaacatgc cgcagagcga cgccgacaaa ctcgtcctgt
    14041 ttaacctgat gccctggccg cgtgaagaag tgatcaacac cactgtgcgc ctgcgcgcca
    14101 gccagtttaa tttgcgggac gatcgcggcc agcctgtgcc gtattttatt cgccatgccc
    14161 gtgagatcga tccaggccta atcgatcggc aaattgttca ttatggcaat tatgatccct
    14221 ttatggagtt cgacatccag atcaaccaga ttgtcccttc tatgggctat cgcacgcttt
    14281 atattgaagc gaatcagcct ggcaacgtga ttgcggcaaa aagtgacgct gaagggattc
    14341 tggaaaatgc tttctggcag attgcgctca atgaggatgg ttctctgcaa ctggtagata
    14401 aagacagcgg tgtgcgctat gaccgggtat tgcaaattga agaaagctct gatgatggtg
    14461 atgaatatga ctattcaccc gcaaaagaag agtgggtaat taccgcggcg aacgcgaaac
    14521 cgcaatgcga tattattcat gaagcctggc agagcagggc tgttatccgt tatgaaatgg
    14581 cggtgccgcg taatctgcaa gaacgcagcg caagacaatc cactggcaga gtaggggtgg
    14641 aaatggttgt cactttgagt gataacagca ggcgcattga tgtggatatc aaccttgata
    14701 accaggctga cgatcatcgt cttcgcgtcc tggtccctac accttttaac accgacagtg
    14761 ttctggcaga tacccagttt ggttcgctaa cgcgccccgt gaacgacagt gcaatgaaca
    14821 actggcagca agaaggctgg aaagaagcgc cggttccggt atggaatatg ctcaactatg
    14881 ctgccttaca ggaagggcgt aacggcatgg ctgtctttag cgaagggtta cgtgaatttg
    14941 aagtcatcgg tgaagagaag aaaacctttg ccattacgtt gctgcgtggc gtgggcttac
    15001 tggggaaaga agatctgctt ttaaggcctg ggcggccttc gggaattaaa atgccagtcc
    15061 cggactcaca actacgtggt ctgctttctt gtcgcctaag tttattgagt tataccggta
    15121 cgccaaccgc cgctggtgta gctcagcagg cgcgagcatg gctgactcca gtacagtgtt
    15181 acaacaaaat cccatgggat gcgatgaagc tcaacaaagc cggattcaac gtgccggaaa
    15241 gttatagttt gttgaaaatg cccccagtgg gatgcctgat aagcgcactt aagaaagctg
    15301 aagaccgaca agaagtgatt ttacggctgt ttaatccggc tgaatcagca acctgtgatg
    15361 cgactgttgc tttcagtcgc gaggtgattt cttgctcaga aacgatgatg gatgaacaca
    15421 ttaccaccga ggaaaatcaa ggttcaaatc tatcggggcc ttttttaccc ggccagtcac
    15481 ggacgttcag ttaccggctt gcctgaatag caatcaaacc gaagccacat atgcgcggcc
    15541 agattgttga caaagggcgc tttgttcatg ccggatacgg catgaacgct ttattcggtc
    15601 tacaaaagca ggcaaattca atatattgca gagatgatgt agaca
//
LOCUS       DAFAVF010000098        15634 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFAVF010000098 DAFAVF010000000
VERSION     DAFAVF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.098"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..217)
                     /locus_tag="LGK42_003013"
     CDS             complement(<1..217)
                     /locus_tag="LGK42_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659239.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLF"
     gene            complement(384..1124)
                     /locus_tag="LGK42_003014"
     CDS             complement(384..1124)
                     /locus_tag="LGK42_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBK2659240.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1217..1852)
                     /gene="argO"
                     /locus_tag="LGK42_003015"
     CDS             complement(1217..1852)
                     /gene="argO"
                     /locus_tag="LGK42_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBK2659241.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(1991..2851)
                     /gene="mscS"
                     /locus_tag="LGK42_003016"
     CDS             complement(1991..2851)
                     /gene="mscS"
                     /locus_tag="LGK42_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBK2659242.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3209..4288)
                     /gene="fbaA"
                     /locus_tag="LGK42_003017"
     CDS             complement(3209..4288)
                     /gene="fbaA"
                     /locus_tag="LGK42_003017"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBK2659243.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4503..5666)
                     /gene="pgk"
                     /locus_tag="LGK42_003018"
     CDS             complement(4503..5666)
                     /gene="pgk"
                     /locus_tag="LGK42_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK2659244.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5716..6735)
                     /gene="epd"
                     /locus_tag="LGK42_003019"
     CDS             complement(5716..6735)
                     /gene="epd"
                     /locus_tag="LGK42_003019"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBK2659245.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7107..7538
                     /locus_tag="LGK42_003020"
     CDS             7107..7538
                     /locus_tag="LGK42_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK2659246.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7561..8139
                     /locus_tag="LGK42_003021"
     CDS             7561..8139
                     /locus_tag="LGK42_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK2659247.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8140..8847
                     /locus_tag="LGK42_003022"
     CDS             8140..8847
                     /locus_tag="LGK42_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK2659248.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8835..9512
                     /locus_tag="LGK42_003023"
     CDS             8835..9512
                     /locus_tag="LGK42_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK2659249.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9506..10183
                     /locus_tag="LGK42_003024"
     CDS             9506..10183
                     /locus_tag="LGK42_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2659250.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(10155..10868)
                     /locus_tag="LGK42_003025"
     CDS             complement(10155..10868)
                     /locus_tag="LGK42_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBK2659251.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10865..11374)
                     /gene="fumE"
                     /locus_tag="LGK42_003026"
     CDS             complement(10865..11374)
                     /gene="fumE"
                     /locus_tag="LGK42_003026"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBK2659252.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(11396..12361)
                     /gene="yggF"
                     /locus_tag="LGK42_003027"
     CDS             complement(11396..12361)
                     /gene="yggF"
                     /locus_tag="LGK42_003027"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBK2659253.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(12358..13635)
                     /locus_tag="LGK42_003028"
     CDS             complement(12358..13635)
                     /locus_tag="LGK42_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBK2659254.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(13650..15038)
                     /gene="cmtA"
                     /locus_tag="LGK42_003029"
     CDS             complement(13650..15038)
                     /gene="cmtA"
                     /locus_tag="LGK42_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBK2659255.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(15066..15509)
                     /gene="cmtB"
                     /locus_tag="LGK42_003030"
     CDS             complement(15066..15509)
                     /gene="cmtB"
                     /locus_tag="LGK42_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBK2659256.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
BASE COUNT         3802 a         4218 c         3773 g         3841 t
ORIGIN      
        1 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
       61 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      121 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      181 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      241 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      301 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      361 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      421 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      481 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      541 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      601 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      661 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      721 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      781 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      841 ggctgggtgc gtaaattcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      901 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
      961 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1021 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1081 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1141 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1201 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1261 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1321 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagccca aagaaccaca
     1381 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1441 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1501 ctgccaacat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1561 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaaga
     1621 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctac
     1681 caccaaaaat ccccgcgcaa atcaggacca aatcgctgat agcgcaaagt aaggcaatca
     1741 taatgtgata ctgacgacga atgccctgat tcatcacaaa agcattttgt gggccgagcg
     1801 gcagaatcat agctgcccca agagcaagac cttgaaagta ataagaaaac acgttggata
     1861 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1921 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     1981 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2041 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2101 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2161 atgcaccaag ttcgttcagg cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2221 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2281 caataataaa ttcgttacgg cgaactggct cgcgggagaa gttaataata tttccggcaa
     2341 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2401 aaatctgcac actcagcaca gtaccggcta cgccgccgag gtcaacatat tctccggcac
     2461 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagacagt gacccctgca
     2521 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgacggac gcggtttgta
     2581 cacccacgcg tcccagtgca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2641 cagagagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2701 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2761 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2821 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2881 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     2941 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3001 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3121 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3181 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3241 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3301 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccagctga ccctgcagat
     3361 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3421 tatcgatgtt cattttcact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3481 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttag
     3541 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3601 aaacaccgtg tacgttaccg aaggacgctg cgatggtgaa acgcgggctg attttgctca
     3661 gctcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3721 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3781 tgccgatttt ggacatacgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3841 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttgccggta gcagcgaagt
     3901 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     3961 cggtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4021 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4081 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4141 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4201 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4261 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4321 cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4381 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4441 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4501 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4561 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4621 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4681 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4741 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4801 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4861 gccggtgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4921 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     4981 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5041 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5101 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5161 gctgccagca gcgggcctgc gcacgcaacg tcagcgaatt taccgatacc gtgagtagaa
     5221 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5281 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5341 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5401 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5461 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt tgcgccttgt
     5521 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5581 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5641 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5701 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5761 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5821 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5881 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     5941 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6001 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6061 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6121 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6181 catggcggag tgaattgtgg tcactgtgcc ggactcaata ccgtacgcat catctaacaa
     6241 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6301 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6361 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6421 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6481 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6541 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6601 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6661 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6721 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6781 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6841 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6901 aatgattgtc cagtcaaaga aagcaattat ctgacttacg tcacgttttc agccccgccc
     6961 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7021 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7081 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcctccg
     7141 cacgctttta cgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7201 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7261 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct cctgaacggt acattcgaag
     7321 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7381 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7441 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7501 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7561 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7621 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7681 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7741 accaatttac tctggggatt gctgaccaac ccgatcgccg ccgcatttgc acccgtcgcg
     7801 atggtcatcg gcctggtcgc cggttgcctg gcgcgagcgg gttggtttcg caccctgcca
     7861 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7921 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     7981 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cggtgatagg cgcgaacctg
     8041 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8101 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8161 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgcca
     8221 atctatagcg cggcgacctt tttctgcctt attgcgctaa aggccacgcg gcggcgggca
     8281 aaatatgtcg tctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8341 tggctaccag aatggctaag cggcacgcca cgctcgccag aacggtggtc gcatgctatt
     8401 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8461 cccgtgcaat gttttattcg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8521 ctgtttgccg ggccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8581 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8641 atgcccgcgt taattattcc gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8701 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8761 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctgacagaa
     8821 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8881 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     8941 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9001 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9061 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9121 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9181 aagcagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9241 gccatcgtca cccggcaaca ctctccggtg gagagacaca gcgcgtggtc atcgcctgcg
     9301 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagccgc ctgactccgc
     9361 aggccagcga aatgctgttg cagcgcttac aacactgggc gtttgaacgt ggttcgctga
     9421 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9481 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat aagatatctt atcgctggcc
     9541 tggcgcggca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9601 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9661 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9721 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9781 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9841 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9901 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
     9961 actctctccg ccactattgc tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10021 gagcgtgttt gaaagctggc tggagaagtg cggccagcga ggcacgagcg tcgtagcaat
    10081 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10141 tctaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10201 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10261 taaaaagctt ctgcgtctgc cagcgatacc cctcccgcca gcttgcgccc taccagtcgt
    10321 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10381 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10441 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10501 caatctcctt ccacgacctg gcgcagattt tccgccagtt tcacaacgtc aaatgtctct
    10561 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10621 ccatccatcg ggagcgtctg aatagcgggc agttccgggt cttgttgcgc gagatattcc
    10681 cagaaggtag ttaaggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10741 cgtcgctgcg aacttaccgc ctgtaaggcc gccagcatac gcagtagcgg tttatgcacg
    10801 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10861 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10921 ccattccgct aaaacgcgcc tctgaccaga cggcgaaatc ggcgaacttc atcgctttta
    10981 acgcatcata gagcgtcgca ttgcaggtta tgctattgac gttgctgata aaatcccagg
    11041 tcatgtcatc ggcaaatccg ggcgtttcat cctgttcgtt caggtaatgc cagtactggg
    11101 aaaagtgcgt gatatcggca tacagccatt tatccatttt ccccagcgca taaatcaaac
    11161 gcagcgcaac atcaatatcg tcaagcgggc cgctttgggc gagtaacggc ttcactgcat
    11221 attcgacgat ctcttcatcg ttatcgacaa acagcgacgg cagaaactgg cgtatcccct
    11281 ggagcaaaat agaatgcgca gttttcataa atgaaaataa attgtcctgt gcatccagtt
    11341 gctcaagcac atcatcttct gtcagcgtcg ccattggtct tccgatagat caccatcaat
    11401 gcagggagtc tattatatta cacgtttggt ccgcgccgcc gatcagtaat gtctgcgtcc
    11461 gcaccccatt cgccgtctgt tggataccgt tcaccaactc gccgcccgtc acgcccgtag
    11521 cgctaaagag gatatcgttc cccctcacca gttcatcgag cgagtacaca cggttaacgt
    11581 caacgcccat tgccttacaa cgcttgcgct cctgctctgc aatctgccga ttttccgtgt
    11641 aatccccttt agcctgacaa aagtcaatca gctcggcctg catatcccca cctaatgctt
    11701 taacggcaca ggcagaaatc acgccttctg gcgcaccgcc gatggtgtac atcacgtcgt
    11761 aaggattatc ctgccagcag gtcagcacgc tggcggcgac atcgccatcc ggcagagcga
    11821 aaaccttcac gcccagctgt gttgcttctt caatcgcggc actcaggcgc ggtttatcca
    11881 gcgtaaccat gcgcagcttg tccagcggtt tacctaacgc ccgcgccacg ttacgcaagt
    11941 tgtccgccag aggcaaagac agatcaatcg ccccggcagc cagtcgatta acaaccagct
    12001 ttttcatata catatcgggc gcatgtaaca ggctatctcg aggggcgaaa gccatgaccg
    12061 ccagcgcatt gctctgcccc atcgctacca tccgcgtgcc ttcaatcggg tcaaccgcga
    12121 tatcgacttc tggcccatcg cctttgccca cctcttcgcc aatccacagc atcggcgcat
    12181 ggtcaatttc tccttcgccg ataaccactc gtccacgaaa agcgacgtcg tttaatgcct
    12241 ggcgcatcgc ggtgaccgcc aggccatcaa ttttattttt gtcgccacat ccggtttgcg
    12301 gccaggcagc caacgctgcc tgttccgtaa cgcgaaataa tggccacgcc agggacatca
    12361 ttgcgcggcc tccccgatat caacgccaaa ttgtgccagc agatatttct cggcctgttc
    12421 attccagatg ccgtgcgttt cctcaaccag ccgcgccaac tctttaaaca gcggatcggt
    12481 tttgcctttt tcggcaaaat cggcaatggc agtgagcggc atggtcacgc cgttataaat
    12541 gagtttttta ccgccaggga tatccggcag attgagcacg gtatctggca ccgcatccag
    12601 cccaccgata tgcgtgacca taaaggacgg ctgcaactgc cccgtggcgc ttaacgcaat
    12661 cgcctctttc atgtcgtccg ttgaaccgcc ggatgtaccg actacgtgcg tgctgttgta
    12721 atggacgttg tagaaattaa acggcacttt gaagttttta tccgtcggcc cggcaaagaa
    12781 gttcagacag ccatcttccg ccagtaattc gtcagccatc tcaacgacag caggcaccgc
    12841 cgcataaaca aaaatgtcat cgaacccgac atctcctgtc agcgcccgca gcgtctggac
    12901 cggatcgctc atccctttgg tattcacata caccagctca atgcctttac tggccgccag
    12961 atccaccggc agcagcttct gcacctgcgc cagacgtttg tcgtcgatat cgaccaccac
    13021 cacccgcgac ggttgtatgc cgccgttaat ggcgtaatcg atagcgccaa tgcccatcgg
    13081 acctgcacac gccagcagtg caatattgcc gccaggtttg acgcccatgc gatgctcata
    13141 aacatattgc gtggtgtgat aattggcatg ataagcacca ataatgcagc acataggctc
    13201 tgccagcgac gccgcagcaa aataagagcc gtgatacggt aatacgcagc ccaaattaat
    13261 ggcgatttcg ggaataatca tataagtggc attgccgcca aaatattcgt agctgtagcc
    13321 cgctgaatat ccgcttggta accccatcgc tggttgcaat acaaaacgct ggcctttttt
    13381 atatttgcca gtgagatttt tacccacttc gacaataacc ccggcacatt catgcccggt
    13441 aatgaccgga tgattttcta aatcgtcggg tacgcgttta tgttcactac cgagtaacgc
    13501 agctttccag gtggataaac agacgctgtc agaaattaca ctcaccagta attcattatc
    13561 ggtaatttct ggcagttcaa attcgcgcag acggacatcc cgcttgccat aaatagcagc
    13621 aactttggtt ttcattttta cctctgtaat cagtgtttat gttcggcggt taattgatta
    13681 aataaagtgt cgagttttgg atcgccaata tagttattaa tcaatatcag cggtttatcc
    13741 gtaacacgtt tcacgcgccc ttccagactg gcatgagtaa cgacgatatc ggcatccgca
    13801 ggcacgtttt ctatcgcgta atgttttact tctattgcca gccccgcttt ttccaggcgt
    13861 ttacggaagg tggtcgcgcc catcgcactg gagcccatcc ccgcatcgca aacaaaggcg
    13921 atacgcttaa ccctgcttaa cgagaatgca ccctcttgtt tcatcgcctt aaccgcattg
    13981 gctgactgag caaactcatc ttcgctctcc gtttccaccg ttttttccat cttcagtatc
    14041 agcgaagtaa tagcaaagga caccagggta cctaccgtaa caccggcaat tgtcgccagg
    14101 aacgagcctt tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc
    14161 accagaccac cgtccagtaa gttaaacatc caggtaccag acataccgcc cgcaatcatg
    14221 gcaataatag tcagcggctt catcagcaca tatgggaaat acagttcgtg gatcccaccg
    14281 aggaagtgaa taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag
    14341 gtaaacgcca gcagcagccc caggcccgga cctgggttag aggccaccat aaagaagatg
    14401 gatttaccgt taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc
    14461 gcattattaa ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa
    14521 cccgcatgta ccagcgcctc gatacactct ttgacgaaag tattggcaat taacaccgcc
    14581 gggccgatca cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag
    14641 ttattgataa ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcgacatat
    14701 tttatgacca acccaccgag cggccccatg atcattgagc caaggaacat cgggatctct
    14761 gcgccaacga tcacaccaat agtacctatt ccgcccatga ccgccccgcg tttaccgccg
    14821 accagatgac cacctgtaga accaatcatc acgggcaata aataggtaat catcgggccg
    14881 acaattttgg cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata
    14941 aaaccccagg caataaaagc accaatattg gggatgacca ttgcagtcaa aaagccccca
    15001 aaagcctgga cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag
    15061 agtaattatc cgcggctgat aatgtccgct aattgttttt ctgatgatgc tgttaagagt
    15121 tgttcgagta tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc
    15181 gaatcggcat cggcagcaga gagtccgatg agtaatttaa ttggttcgtc attccccgga
    15241 aaataaacac cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc
    15301 ggtcgcgcat gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg
    15361 gtggaatctt taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat
    15421 accatcgaga aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat
    15481 gattctggaa aataatcact aagccgcata aattatcctt attttatttt tagggtaccg
    15541 tccggcggta aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg
    15601 atgttttgaa aacagtgaat tattaatttc ttat
//
LOCUS       DAFAVF010000099        15619 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFAVF010000099 DAFAVF010000000
VERSION     DAFAVF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.099"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(61..504)
                     /gene="mioC"
                     /locus_tag="LGK42_003031"
     CDS             complement(61..504)
                     /gene="mioC"
                     /locus_tag="LGK42_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBK2659257.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(594..1052)
                     /gene="asnC"
                     /locus_tag="LGK42_003032"
     CDS             complement(594..1052)
                     /gene="asnC"
                     /locus_tag="LGK42_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBK2659258.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            1204..2196
                     /gene="asnA"
                     /locus_tag="LGK42_003033"
     CDS             1204..2196
                     /gene="asnA"
                     /locus_tag="LGK42_003033"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBK2659259.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(2201..3652)
                     /gene="viaA"
                     /locus_tag="LGK42_003034"
     CDS             complement(2201..3652)
                     /gene="viaA"
                     /locus_tag="LGK42_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBK2659260.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3646..5142)
                     /gene="ravA"
                     /locus_tag="LGK42_003035"
     CDS             complement(3646..5142)
                     /gene="ravA"
                     /locus_tag="LGK42_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBK2659261.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            5365..7233
                     /gene="kup"
                     /locus_tag="LGK42_003036"
     CDS             5365..7233
                     /gene="kup"
                     /locus_tag="LGK42_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBK2659262.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            7400..7819
                     /gene="rbsD"
                     /locus_tag="LGK42_003037"
     CDS             7400..7819
                     /gene="rbsD"
                     /locus_tag="LGK42_003037"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK2659263.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            7827..9332
                     /gene="rbsA"
                     /locus_tag="LGK42_003038"
     CDS             7827..9332
                     /gene="rbsA"
                     /locus_tag="LGK42_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBK2659264.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            9337..10302
                     /gene="rbsC"
                     /locus_tag="LGK42_003039"
     CDS             9337..10302
                     /gene="rbsC"
                     /locus_tag="LGK42_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBK2659265.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            10327..11217
                     /gene="rbsB"
                     /locus_tag="LGK42_003040"
     CDS             10327..11217
                     /gene="rbsB"
                     /locus_tag="LGK42_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBK2659266.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            11343..12272
                     /gene="rbsK"
                     /locus_tag="LGK42_003041"
     CDS             11343..12272
                     /gene="rbsK"
                     /locus_tag="LGK42_003041"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK2659267.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            12276..13268
                     /gene="rbsR"
                     /locus_tag="LGK42_003042"
     CDS             12276..13268
                     /gene="rbsR"
                     /locus_tag="LGK42_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBK2659268.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAISAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(13234..14661)
                     /gene="mdtD"
                     /locus_tag="LGK42_003043"
     CDS             complement(13234..14661)
                     /gene="mdtD"
                     /locus_tag="LGK42_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBK2659269.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(14684..15376)
                     /locus_tag="LGK42_003044"
     CDS             complement(14684..15376)
                     /locus_tag="LGK42_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK2659270.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         3761 a         3837 c         4098 g         3923 t
ORIGIN      
        1 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
       61 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
      121 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
      181 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc
      241 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
      301 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
      361 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
      421 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
      481 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
      541 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
      601 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
      661 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatc
      721 acttttataa agatgctgta gtggccggtt gtgtaatagg cttcagtgac ttcatcaagg
      781 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
      841 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
      901 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
      961 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
     1021 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
     1081 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
     1141 ttattgcatg tgtgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
     1201 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
     1261 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
     1321 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
     1381 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
     1441 accttagggc aacacgactt cagcgcgggc gaaggactgt acacgcacat gaaagccctt
     1501 cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
     1561 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
     1621 atctgggcgg gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg
     1681 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
     1741 ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc ttggcgcggt attccttgtc
     1801 gggattggcg gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat
     1861 gactggagca ccccgtcaga gctgggtcat gcgggtctga acggcgatat tctggtgtgg
     1921 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
     1981 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctggagct ggagtggcat
     2041 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
     2101 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
     2161 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
     2221 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
     2281 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
     2341 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
     2401 ccaccgcgtc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
     2461 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
     2521 tgccttgtgg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
     2581 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
     2641 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
     2701 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
     2761 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
     2821 ccagttccgt tatccctagt gtcgccagtt ctggcggcag gagacgtaaa atatcatcgc
     2881 tttgttgcag accatcaacc tgctcaggaa cagttgccgg ttcgcgcacc atggtgcgga
     2941 aggcttccat ctgcgcatcg ttacgcggta ttgatttggc ttcccgggaa cgccccagct
     3001 gttctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
     3061 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcgg
     3121 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcctgaactt
     3181 cacttaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
     3241 cgatcagact taaacgccaa cgctggagga aaagcgtatg taacgctgaa gtgatcgtgc
     3301 tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
     3361 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
     3421 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
     3481 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagt cgtgggaatt
     3541 tttcaaagaa gactgccagc tgcggtgagg ccagcagcgc gatgatcatc tcttcgatca
     3601 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
     3661 ctggcgaatc tgtgcgccga catcctgtag gctggcttca atgcgaccta accagtcgcc
     3721 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcact
     3781 ttcaagctct tccagttgtt gttttatttc acctggcaga ccttcagccg atgaaccggg
     3841 aagtgccagc gtactgcctt gtaaacttac atcgcgtaca acaagatgtt gggcgctatc
     3901 cacttccaga ttcaatttct gggcaaagcc gataccgttc agtttgccgc gaatttcacc
     3961 acctttactc agccactgtt ccagcgcgtt acgctcaaag gagatatgaa ccacttccat
     4021 atcatgcaat tttaacggtt tttgcagcag cagagtcaga gtggaagcag taacgttaac
     4081 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
     4141 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
     4201 agtcaacatc ccttgctgtt gccaggcgtg accggtcatc aatacatcaa tttgttgttg
     4261 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
     4321 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataagc ggatcgcttt
     4381 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gctggcgcag
     4441 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
     4501 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
     4561 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
     4621 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
     4681 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
     4741 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
     4801 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
     4861 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
     4921 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
     4981 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
     5041 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
     5101 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
     5161 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
     5221 tcaaaggggg ggtatttttc tttgagccag gttaatgtgg ccgcatttag gagtacgatt
     5281 ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc aagggactaa gcacacattt
     5341 catatttcaa cgaaagacta gtctatgagc actgataata agcaatcatt gcccgcgatt
     5401 accctcgcgg cgattggagt tgtctacggc gatattggta ccagcccgtt atatacactt
     5461 cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt gtttggcttt
     5521 ttatcgctga tcttctggct gctaatcttt gtggtttcca ttaaatatct caccttcgtg
     5581 atgcgggcag ataacgccgg tgaggggggg atcctgacgt tgatgtcgct tgccgggcgt
     5641 aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg cggcagcttt
     5701 ttctatggtg aagtcgtcat aacacccgct atttcggtga tgtcagccat tgaaggtctg
     5761 gaaatcgtcg ccccgcagct ggatacctgg atagttcccc tctcaattat cgttctcact
     5821 ttattattta tgattcaaaa acatggcacg gctatggtcg gtaagctgtt tgcgccgatc
     5881 atgctgacct ggtttttgat tctggcagga ctggggttac gtagcattat tgctaacccg
     5941 gaagtgctgc atgcgctgaa tccaatgtgg gcggtgcatt tcttccttga atacaaaacg
     6001 gtttctttta ttgcattagg ggcagtggtg ttgtcgatta cgggggtcga ggcgctgtat
     6061 gctgatatgg ggcactttgg taagttccct attcgcctgg cgtggttcac cgtcgtatta
     6121 ccttccttga cccttaatta cttcggtcag ggagcgctgt tgttaaagaa cccggaagcg
     6181 attaagaacc cgttcttcct gttggcaccg gactgggcgc tgatcccgct gctgatcatc
     6241 gccgcactgg cgacggtaat tgcctcgcag gcggttatct ctggcgtctt ctcattgacg
     6301 cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca ttattcacac ctccgaaatg
     6361 gagtcagggc aaatctatat tccgtttgtg aactggatgc tctatgtcgc ggtcgtgatt
     6421 gtgattgtca gctttgagca ctccagcaac ctggcggcgg cgtacgggat tgcggtgacc
     6481 ggaaccatgg tgctgacgtc tattctctcg actaccgtgg cacgtcagaa ctggcactgg
     6541 aataagtatt ttgttgcgct gatcctgatt gctttccttt gtgtcgatat tccattgttc
     6601 accgctaacc tcgataaact gctctccggc ggctggttgc cattgagcct cggtactgtg
     6661 atgtttatcg tgatgaccac ctggaagagc gagcgtttcc gcttgctgcg gcggatgcat
     6721 gaacatggta actctctgga agcgatgatt gcttcgctgg agaaatcacc gcctgttcgc
     6781 gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg tcattccctt tgcgctgatg
     6841 cataacctta aacataacaa ggtattgcat gagcgggtga ttctgttaac tctgcgtacc
     6901 gaagacgcgc catatgtcca taatgtccgt cgggtacaga ttgaacaact gtcgcccact
     6961 ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc caaacgtgga agaagttttc
     7021 caccgctgcg gtctggaagg attaagttgc cggatgatgg aaacctcctt ctttatgtcg
     7081 catgagtcgt tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg caagctgtac
     7141 ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat ttgaaattcc gccaaacagg
     7201 gttatcgaac tgggtactca ggtcgaaatc taacgccaga cgcctccttt ctccataagg
     7261 gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg atcacatttc
     7321 cgtaacgtca cgatggtttt cccaactcag tcaggattaa actgcgggtc agcgaaacgt
     7381 ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata tttcatcggt
     7441 gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt tacccatccc
     7501 caaaagtaca acgcgtatcg atatggcatt aacccagggt gtaccttctt ttatgcaggt
     7561 gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg aagagatcaa
     7621 acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc tgcaaaaaca
     7681 ccagggaaat accattgaaa ttcgttacac cacgcatgaa caattcaaac aacaaaccgc
     7741 agaaagtcag gcggtaattc gcagcggaga atgttctccg tatgcgaata tcattctctg
     7801 tgctggcgtg acgttctgag gccgtcatgg aagcattact tcagcttaaa ggcatcgata
     7861 aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat ccgggccgcg
     7921 tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa gtgcttactg
     7981 gtatctatgc tcgcgatgcc ggcacgcttt tatggctggg gaaagaaacg acatttaccg
     8041 ggccgaaatc ttcccaggaa gccgggattg ggattatcca tcaggaactg aacctgatcc
     8101 cgcagttgac cattgccgaa aacattttcc tcggtcgtga gtttgttaat cgctttggca
     8161 aaattgactg gaaaaccatg tatgccgaag cggataaatt gctggctaaa cttaacctgc
     8221 gctttaaaag cgacaagctg gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa
     8281 tcgccaaagt gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc
     8341 tgaccgatac cgaaaccgaa tccctgttcc gcgtcatacg cgagctgaaa tcgcaaggcc
     8401 gcggtattgt ctatatctcc caccgcatga aagaaatctt cgagatttgc gatgacgtta
     8461 ccgtttttcg tgacgggcaa tttattgctg agcgcgaagt ggcatcactg accgaagatt
     8521 cgctgattga gatgatggtg ggccgcaagc tagaagatca atatccacac ctggacaaag
     8581 cgccgggaga tatccgcctg aaagtcgata atctctgcgg acctggcgtt aacgatgtct
     8641 cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta
     8701 ccgaactgat gaaagtgctc tacggcgcac taccgcgcac cagcggttac gtcaccctgg
     8761 atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaagcggc attgtgtata
     8821 tctccgaaga ccgtaaacgt gacggtttag tgttgggcat gtcagtaaaa gagaacatgt
     8881 cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac
     8941 agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacaccgtcg atggaacagg
     9001 caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga
     9061 cacgccccaa agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc ggcgcgaaaa
     9121 aagagatcta tcaactgatt aaccagttca aagccgatgg cttgagcatc attctggtgt
     9181 catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg catgaagggc
     9241 atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg gctgccgctg
     9301 tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac tgtctctggt
     9361 cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc tctgctggtg
     9421 ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa cttattcaat
     9481 attctccagc aaacctcggt gaacgccatt atggcggtcg ggatgacgct ggtgatcctg
     9541 acgtcgggca tcgacttgtc tgtaggttct ctgttggcgc taaccggcgc agttgctgca
     9601 tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgccgctct cgcgttaggt
     9661 gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgttca ggcgtttatc
     9721 gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac caacggtagc
     9781 ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg tattggtcgt
     9841 ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc ggcctggtac
     9901 atgctgcatc acacgcgtct ggggcgttac atctacgcgc tgggcggcaa cgaagcggca
     9961 acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca tcgtctattc tctttgtggt
    10021 ctgctggcat cgctggccgg gatcattgaa gtggcgcgtc tctcctccgc acaacccacg
    10081 gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg tacgagtctg
    10141 gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct tggcttcctt
    10201 aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt caaagcggtg
    10261 gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact acaggacatc
    10321 ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct aagcgccacc
    10381 gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac gcttaacaac
    10441 ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct tggctataac
    10501 ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt gcaggactta
    10561 accgttcgcg gcaccaaaat tctgctgatt aacccgaccg actccgacgc agtaggtaat
    10621 gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg ccaggcaacg
    10681 aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa aatcgccggt
    10741 gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca aggcattgct
    10801 ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc tgctcacaag
    10861 tttaatgttc ttgccagcca gccagcagat tttgatcgca ctaaaggttt gaacgtaatg
    10921 cagaacctgt tgaccgctca tccggatgtt caggctgtat tcgcgcagaa tgatgaaatg
    10981 gcgctgggcg cgctgcgcgc actgcaaact gccggtaaat cggatgtgat ggtcgtcgga
    11041 tttgacggta caccggatgg cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc
    11101 gctcagctac ccgatcagat tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa
    11161 ggcgagaaag ttcaggctaa gtatccggtt gatctgaaac tggttgttaa gcagtagttt
    11221 taatcaggtt gtatgacctg atggtgacat aaatacgtca tcgacagatg aacgtgtaat
    11281 ataaagaaaa gcagggcacg cgccacccta acacggtggc gcattttatg gacatcccga
    11341 atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat taatgctgac cacattctta
    11401 atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg taaccactat caggttgcat
    11461 ttggcggcaa aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg aatatcgcgt
    11521 ttattgcctg tacgggtgat gacagcattg gtgagagcgt tcgccagcag ctcgccactg
    11581 ataacattga tattactccg gtcagcgtga tcaaaggcga atcaacaggt gtggcgctga
    11641 tttttgttaa tggcgaaggt gagaatgtca tcggtattca tgccggcgct aatgctgccc
    11701 tttccccggc gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca gcattattaa
    11761 tgcagctgga atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc catcaaaata
    11821 agactatcgt tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa ctgctggcgc
    11881 tggtggacat tattacgcca aacgaaacgg aagcagaaaa gctcaccggt attcgtgttg
    11941 aaaatgatga agatgcagcg aaggcggcgc aggtactgca tgaaaaaggt atccgtactg
    12001 tactgattac tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa ggtcagcgcg
    12061 ttcctggatt ccgggtgcag gctgtcgata ccattgctgc cggagatacc tttaacggcg
    12121 cgttaatcac ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg tttgcccatg
    12181 ctgccgctgc gattgccgta acacgtaaag gcgcacaacc ttccgtaccg tggcgtgaag
    12241 agatcgacgc atttttagac aggcagaggt gacgcttggc tacaatgaaa gatgttgccc
    12301 gcctggcggg cgtttctacc tcaacagttt ctcacgttat caataaagat cgcttcgtca
    12361 gtgaagcgat ttccgccaaa gttgaagcgg cgattaaaga actcaattac gcgccatcag
    12421 ccctggcgcg tagcctcaaa ctcaatcaaa cacataccat tggcatgttg atcactgcca
    12481 gtaccaatcc tttctattca gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg
    12541 gttatagtct cgtcctttgc aataccgaag gcgatgaaca gcggatgaat cgcaatctgg
    12601 aaacgctgat gcaaaaacgc gttgatggct tgctgttact gtgcaccgaa acgcatcaac
    12661 cttcgcgtga aatcatgcaa cgttatccga cagtgcctac tgtgatgatg gactgggctc
    12721 cgttcgatgg cgacagcgat cttattcagg ataactcgtt gctgggcgga gacttagcaa
    12781 cgcaatatct gatcgataaa ggtcataccc gtatcgcctg tattaccggt ccgctggata
    12841 aaactccggc gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca
    12901 gcattcctga tggctatgaa gtcactggtg attttgaatt taacggcggg tttgacgcca
    12961 tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta
    13021 tggctgttgg cgtttaccag gcgttatatc aggcagagtt acaggttccg caggatatcg
    13081 cggtgattgg ctatgacgat atcgaactgg caagctttat gacgccacca ttaaccacta
    13141 tccaccaacc gaaagatgaa ctgggggagc tggcgattga tgtactcatc catcggataa
    13201 cccagccgac ccttcagcaa caacgattac aacttactcc gattctgatg gaacgcggtt
    13261 cggcttagat ttacgctgtc ttttgatcaa attattacca tcggttgttt tcagaagcat
    13321 gaacattgct gctgaagcaa cagtaataat gcccatcgtg ataaacgtat agtggaattg
    13381 ttcgacagtc gttgtgcctt ccatcccttc ataaacgcga aggacggccg cacttacagc
    13441 aacgcctaaa ctaatcgaca gttgctgcgt gaccgccaga acactgttac cgctgctggc
    13501 gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc gcggtaaatt gcgtcgacat
    13561 agccatccct aatataaaca acggcaagat cagcatccat atcgccattg ccggtgattg
    13621 caaagagaac tgagcgatca ttagcccaat aatcaccgtg atccccacta acgtatggcg
    13681 atagcccaga cgacgtaaga cttgggtaac catcgatttt gcaataatgg aacctaacgc
    13741 tgtcggtgcc atcatacagc cggcaataaa cgcctgataa ccaaatccta cctgtaacat
    13801 caatggcata aggaacggta cacaaccggt ccccagacgg gttgcaatat tgcctacgat
    13861 accgatcgag aaagtgcggg ttttaaataa atctaatgaa attaatgggt ttggcgtgtg
    13921 tcgcgcatgg agaatataga gaagcagtaa cccgatgctg gtgacaatta ccgtcaaggc
    13981 aatccagctg gcgacaatcc tttccccgaa tagctctatt ccgcttgaga agagaacaag
    14041 gctgaggcca aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg tggtgaaatt
    14101 gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat tgattaaaaa
    14161 tatccagtgc caggttgccc aggtgaccag cacgccgcca agaacggggc ctaaaattgg
    14221 ccccaccaga cccggcatgg cgacaaaatt caatactgga agaagttcat tacgaggata
    14281 agcgcgcagt aaggccagcc gagcaacagg catcatcatt gcgccgccta tcccctgaat
    14341 aacccggaag acaaccagct gtggtagcga attagaaagt gcgcaggcca gagaacccaa
    14401 tgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat cggctagcca
    14461 tccgcttacc ggaataagca tcgccaccgt cagcgtataa ctgatgatgg ctgattgcat
    14521 cgcgagagga gaacgattaa ggctatgagc gattgcgggt aaggcggtat tcagaatagt
    14581 ggcatcaagt gcctgcatga agaaggccat cgccgcgatc cacggcaaac ccgccatact
    14641 gcgcttcttt ttatcgctca ttcaatgtcc tgttatcggg ttgtcactta tcaggtgagc
    14701 gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc gctttggaca atcgcatcga
    14761 caatcgcctg gtgcagatcc agctttatca ctgtgtcgct ggtaattgac gtgaagtaag
    14821 tgtgataaac cgaatggaat agcgaggcga atgaggtcaa aaacggattg gcgctcattt
    14881 catagatatg ctcatgccag gccatatcga cttcgatcca gcgttcacgg cgaaagttct
    14941 cttttaatgc cgccatttcg gccattaacg tattgagatg cgccttctgt tccgcggtgc
    15001 caaccgttgc agccagtagg caagcttgcg gctccagaca gatacgcata accagaaagt
    15061 gatcgatgac ctgatgaaag ttctcttctg tcatccacca ggtaagcaat tcctgatcaa
    15121 gaaaattcca gtttgattgt ggcatgaccc gggtaccaat tcgcggtcgc ggtaaaacca
    15181 tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc actccaaatt
    15241 gctcgcccag ctcaatctca ccaggcaaaa tggtgccggg ttcatattca ccttttaaga
    15301 tgcgttgcgc cagcttctca gccagaacat acgaaaggtt tttctgtgca gctaactgtt
    15361 gtgcgcttaa aggcattact aatcttcctt tttcttttta ttcctcctta gtatgccacc
    15421 aggaagtgtg attacggttg caaaaacggc aaattgcttg ttttatggca cattaacggg
    15481 gcttttgctg aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
    15541 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc
    15601 agcggggttc tcctgagaa
//
LOCUS       DAFAVF010000100        15471 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFAVF010000100 DAFAVF010000000
VERSION     DAFAVF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.100"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            66..668
                     /locus_tag="LGK42_003045"
     CDS             66..668
                     /locus_tag="LGK42_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBK2659271.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(670..1191)
                     /gene="ruvC"
                     /locus_tag="LGK42_003046"
     CDS             complement(670..1191)
                     /gene="ruvC"
                     /locus_tag="LGK42_003046"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBK2659272.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1226..1966)
                     /locus_tag="LGK42_003047"
     CDS             complement(1226..1966)
                     /locus_tag="LGK42_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2659273.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1995..2447)
                     /gene="nudB"
                     /locus_tag="LGK42_003048"
     CDS             complement(1995..2447)
                     /gene="nudB"
                     /locus_tag="LGK42_003048"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBK2659274.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAVREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2440..4212)
                     /gene="aspS"
                     /locus_tag="LGK42_003049"
     CDS             complement(2440..4212)
                     /gene="aspS"
                     /locus_tag="LGK42_003049"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK2659275.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4522..5088
                     /locus_tag="LGK42_003050"
     CDS             4522..5088
                     /locus_tag="LGK42_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK2659276.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5085..5903
                     /locus_tag="LGK42_003051"
     CDS             5085..5903
                     /locus_tag="LGK42_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK2659277.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLVQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5956..6351
                     /locus_tag="LGK42_003052"
     CDS             5956..6351
                     /locus_tag="LGK42_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBK2659278.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6392..7135
                     /gene="cmoA"
                     /locus_tag="LGK42_003053"
     CDS             6392..7135
                     /gene="cmoA"
                     /locus_tag="LGK42_003053"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBK2659279.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7132..8103
                     /gene="cmoB"
                     /locus_tag="LGK42_003054"
     CDS             7132..8103
                     /gene="cmoB"
                     /locus_tag="LGK42_003054"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBK2659280.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8268..10697)
                     /gene="torZ"
                     /locus_tag="LGK42_003055"
     CDS             complement(8268..10697)
                     /gene="torZ"
                     /locus_tag="LGK42_003055"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBK2659281.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10722..11822)
                     /locus_tag="LGK42_003056"
     CDS             complement(10722..11822)
                     /locus_tag="LGK42_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBK2659282.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGAD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12210..12956)
                     /gene="cutC"
                     /locus_tag="LGK42_003057"
     CDS             complement(12210..12956)
                     /gene="cutC"
                     /locus_tag="LGK42_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK2659283.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDSLQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(12970..13536)
                     /locus_tag="LGK42_003058"
     CDS             complement(12970..13536)
                     /locus_tag="LGK42_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2659284.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13752..>15471
                     /gene="argS"
                     /locus_tag="LGK42_003059"
     CDS             13752..>15471
                     /gene="argS"
                     /locus_tag="LGK42_003059"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK2659285.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIET"
BASE COUNT         3652 a         4085 c         3781 g         3953 t
ORIGIN      
        1 ctcctgaaaa aataaatttt ttatgctatt acgtatattc gtatcgattt caatggaatg
       61 acaacgtgaa tattaattac cctgctgaat atgaaattgg agatatcgtc tttacatgta
      121 taggtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt aatcacgtcg
      181 ggatcattat cggtcataac ggtgaagact tcctggttgc agaaagccgg gttccccttt
      241 caaccatcac cacgctatct cgttttatta aacgttcgtc taatcaacgc tatgctataa
      301 agcgattaga cgctgggctg acagaacaac aaaaacaacg aattgttgaa caggttcctt
      361 cccggctacg caaactttac cacacgggtt ttaaatacga atcttcccgc cagttctgtt
      421 caaaatttgt ctttgatatt tataaagagg cgctatgtat tccggtgggt gaaatagaga
      481 cgtttggaca actgttaaat agcaatccga atgcaaaact cactttctgg aaattctggt
      541 tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg tggcatcatc
      601 cgggtttggt gttgattcac gcggagggag ttgaaacgcc tcagcctgaa ctgaccgagg
      661 cggtataact taacgcagtc gccctctcgc caggttcagc cgcgattcgc tcatctgcat
      721 cgcattctga ctgacgtgac aatgggtaat cgcgatagct aacgcatcgg cggcatccgc
      781 ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct ggcttttttc
      841 ggcactacca atacctacca ccgtttgctt tacctgacgt gccgcgtatt caaataccgg
      901 caattcctga ttcaccgccg caacaatcgc cacgccgcgc gcctggccca gtttcagggc
      961 tgagtcagcg ttcttcgcca taaagacttg ttcaatggcg aaataatcag gctggaactg
     1021 ggtgatgatt tccgtcacgc ccgcatagat gagcttcaga cgagacggta aatcatccac
     1081 tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctgcctacct ggcgaattac
     1141 gccatagccg gtcacgcgcg aacccggatc aatgccgaga ataatagcca tcacgcgtct
     1201 ccgttttgct gtttagcagg cctcatcaga gagtcgctgc gacctcatca gagatttcac
     1261 cgttatggta aacttcctgc acgtcgtcgc aatcttccag catatcgatc agacgcatca
     1321 gtttcggtgc ggtttctgca tccatatcag ctttggtgga cgggatcatg gaaacttccg
     1381 cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg cactttaccc atttcttccc
     1441 atgcagtgta gacatcaatc gcgccgtcat cataggtcac aacgtcttca gcacctgctt
     1501 ccagagctgc ttccatgatg gtgtcttcat cgcctttctc gaaggagatc acgccttttt
     1561 tgctgaacag ataggctacg gaaccatctg taccgaggtt accgccacat ttgctgaatg
     1621 catgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca atcatgattg
     1681 ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgtttgca tcatcatcac
     1741 cgcccacacc acgtgcaatt gcgcggttca gtgtgtcgcg ggtcatgttg ttagacagtg
     1801 ctttatcaat tgctgcacgc agacgcgggt tagcgtccgg atcgccaccg cccagcttag
     1861 ccgcggttac cagctcacga atgattttag tgaagatttt accgcgcttt gcatcctgcg
     1921 cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa atatctccag
     1981 atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg ccggttgctc
     2041 caggacttag tgagcgccgc cgcagcaggc gcatcaagcc acttgtaagc cagatgttca
     2101 gtgaaaacga tctgccgctc gtgtgggagc gcaagacaga accatgattc cgtattacgc
     2161 gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc taccgtgcgc
     2221 tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtgacctc ttcctttact
     2281 tcgcgcacgg cagcttgcgg cgcggtttcc ccctcttcca cgctgccggt taccgactgc
     2341 cagaaatcgg gatcgtcacg ccgctgcaac atcagcaccc gtttcgtatc ttgtgcgtag
     2401 atgaccacta agatcgaaac gggacgctta taagccatat cagttattct cagccttctt
     2461 cacaacctga atgctcagct cagccagtgc agtcgggtta gcaaagctcg gtgcttcagt
     2521 catcagacac gctgccgccg tggttttcgg gaaggcaata acgtcacgga tattgtcggt
     2581 gccggtcagc agcatggtca gacggtcaag accgaatgcc agacctgcgt gcggcggagt
     2641 accgtatttc agagcgtcga gcaggaagcc gaatttctcg cgctgttcct cttcgttgat
     2701 acccagaata ccaaacaccg tctgctgcat atcaccatta tggatacgta ctgaaccacc
     2761 gcccacttcg taaccattga tgaccatatc gtaagcgttc gccaccgcat tttccggtgc
     2821 agctttcagt tctgcagcag tcatgtcttt cggtgaggtg aacggatggt gcattgccgt
     2881 caggccgcct tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca gcggtgccca
     2941 tttgctttcg tcggtcagac caaggtcttt acccactttc aggcgcagtg cgcccatcgc
     3001 gtcggcaaca attttcttgt tgtcggcacc gaagaaaatc atatcgccat cttgcgcggc
     3061 agtacgatcc aggatggctt cgatgatttc tgcattaagg aacttcgcta ccgggctgtt
     3121 gataccttcc agacctttcg cgcgttcgtt aactttgatg taagccagac ctttcgcgcc
     3181 gtagatttta acgaagttac cgtattcgtc gatctgctta cgggtcagcg atgcgccgcc
     3241 cggaacgcgc agagcggcaa cgcgaccttt cggatcgttc gccggacctg caaatactgc
     3301 aaactcaaca gatttcagca gatcagcaac gtcggtcagt tccatcgggt tacgcagatc
     3361 cggtttatca gaaccataac ggcgttctgc ttccgcaaag gtcattaccg ggaaatcgcc
     3421 cagatccacg cccttcactt ccagccacaa atgacgcacc agcgcttcca tcacttcacg
     3481 cacttgcggc gcggtcatga aagaagtttc cacatcgatc tgagtaaatt caggctgacg
     3541 atcagcgcgc aggtcttcgt cacggaagca tttaacgatc tgatagtaac ggtcaaaacc
     3601 ggacatcatc agcagctgtt tgaacaactg tggggattgc ggcagtgcgt agaatttacc
     3661 tttgtgcaca cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag
     3721 catcggagtt tcgatgtcga ggaagccgtg gtcatccata aaacggcgca ccaggctggt
     3781 gattttagcg cgggttttca ggcgctgcgc catttccggg cgacgcaggt cgaggtagcg
     3841 gtatttcaga cgcgcttctt cggtgttgac gtggttagag tcaagcggca gaacatctgc
     3901 gcggttgatg atagtcagcg aggacgccag cacttcaatt tcgccggtcg ccatatcgcg
     3961 gttaatattt ttttcgtcac gcgcacgtac ggtgcccgta acctgaatgc agaactcatt
     4021 acgcagttca gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac
     4081 gataccttcg cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac gacgacggtt
     4141 gacccaacca cacagagtca cctgctgccc cacgtgggac aaacggagct gtccacaata
     4201 ttctgtacgc atgagatatc ccttaactta gctgccggcg gatgccccct gctgcgcagg
     4261 tgaccaagtc gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg gctattatac
     4321 tggaaattct gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt taccacttta
     4381 tatcgggcct gaaatcagac tctacgccag tttgctataa aggtgttgcc cgaactcata
     4441 aaaattaaca aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt gaggcacgat
     4501 tttgtttttt caggagtcag catgcttgaa cttaatgcta aaaccactgc gttggtggtg
     4561 atcgatttac aagaaggcat cttacctttt gccggtggtc cacatactgc cgatgaggta
     4621 gttaatcgcg ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg
     4681 gtgcgcgttg gctggtctgc cgatcacgcc gaagcattaa aacagccggt tgatgccccc
     4741 tcccccgcta aagtgttgcc cgaaaactgg tggcaacatc cagctgcatt aggtgcaact
     4801 gacagtgata tcgaaatcat caaacgtcag tggggtgcgt tttacggtac ggatctggag
     4861 ttgcaactgc gccgccgagg tatcgataca atagtgttat gtgggatctc gaccaatatc
     4921 ggtgttgaat ccaccgcccg caatgcctgg gaacttggct ttaatctggt gattgccgaa
     4981 gacgcctgta gtgccgccag cgccgagcag cacaataaca gcatcaacca tatctaccca
     5041 cgcatcgccc gtgtgcgtag cgtggaagag atccttcacg cgttatgatt tacatcggtc
     5101 taccgcaatg gtcgcatcct aaatgggtgc ggttgggaat caccagcctt gaagagtatg
     5161 cccgccactt taactgcgtg gagggcaaca ccacgcttta cgccctgccg aaacccgagg
     5221 ttgtcctgcg ctggcgtgaa cagaccacag atgacttccg cttctgtttt aagtttccgg
     5281 cgaccatttc gcatcaggca gcattacgac attgcgatga tttagtgact gaatttttga
     5341 cccgcatgtc accgttggct ccgcgcattg ggcaatactg gctgcaactg cctgccacat
     5401 tcggcccacg ggagctgcct gcgctttggc attttctcga ttctctccct ggcgaattta
     5461 attatggtgt ggaagtccgc catccacagt ttttcgccaa aggggaagag gaacaaacgc
     5521 ttaatcgcgg tttacatcag cgcggcgtta atcgggtgat tttagacagc cgcccggttc
     5581 atgcagcacg tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc
     5641 cggtacatgc tgtactgacg gcgacaaatc cgctgatccg ttttatcggt agtgatgata
     5701 tgacgcaaaa ccgggaatta tttcaggtct ggttacaaaa attagtgcag tggcatcaga
     5761 ccactacgcc ttatcttttt ttacatacgc cagacattgc ccaggccccg gaactggtac
     5821 ataccctgtg ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc
     5881 cacagcaatc ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt
     5941 ttaaagggag tttgtatggt aagcgcgctg tatgccgttt taagtgcgtt gttattaatg
     6001 aagttctctt ttgatgtcgt tcgcttgcgt ttgcagtacc gcgttgccta tggtgacggt
     6061 ggttttagcg aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc
     6121 atcgcgattg tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat
     6181 atttgcggca tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt
     6241 ctgttccgct ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg
     6301 ctggcgaatc tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata
     6361 cgccactttc tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc
     6421 ccctatcgcc agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga
     6481 tatgatccag cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc
     6541 cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc
     6601 gacgctctcg gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa
     6661 ctccccggcg atgattgaac gctgccgtcg tcatattgac gcctataaag cccctacgcc
     6721 agtagacgtc attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt
     6781 gctgaatttt accctgcaat tcctggaacc ttccgagcgc caggcattac tggataaaat
     6841 ttatcaagga ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga
     6901 tgccaaagtt ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta
     6961 cagcgaactg gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc
     7021 cgtggaaacc cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt
     7081 ccagtgcttt aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac
     7141 tttggtaact tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg
     7201 cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc
     7261 aacgcggtgg aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta
     7321 accgccgaaa gcgaagagcc actgagcacc gggcaaatta aacgcattga aacgcttatg
     7381 cggaacctga tgccatggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc
     7441 gaatggcgtt ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga
     7501 cgcaccattc tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca
     7561 ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg
     7621 gtgcgtaaac tactgggtaa cgatcagcgc gcacatttgt taccgttagg tattgaacag
     7681 cttccggcac tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt
     7741 tcgccgctgg agcatctctg gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg
     7801 ctggaaacgc tggttattga tggcgatgag aacacggtgc tggtaccagg cgatcgttac
     7861 gcgcaaatgc gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag
     7921 aagtgtggtt ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag
     7981 cgacgcactg aatggatggt taccgaatct ctgtccgatt tcctcgaccc acatgatccg
     8041 agtaagacag ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg
     8101 taaaggtctg gtattactgc cggatgcggc ttgaatgcct tatccggcct acaaaccctt
     8161 gctaattcaa tctattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagtt
     8221 ttacgtttgc ataacctcag cgcccgtttc cgggcgctat tcacttctta tgcctgaata
     8281 gctggctgat caaacgccgt taacttcggc gcattgcccg tatatttttc gatatacacc
     8341 agcgcagagt taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac
     8401 acgttcgcac tgccgttttt acacaagcca ttttccagat ctggccatgc accttcatgc
     8461 acgcatacca cgcctttttt gatcccgtca gtcaccaccg cgcctgtcag aatctgacca
     8521 cgtttgttcc acacacgcac cagatcacca ttcgcaatac caaagcgagc agcatcttcg
     8581 gtgtgaatag tgattggttc acgatctgca accgcatatt ttttacgcag ttccgcatag
     8641 ttaagctgac tatgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg
     8701 tcggcggtac ccttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta
     8761 tagccaaatt tttccagcgt tttggagtaa atctcaattt tgccgcttgg cgtacccagc
     8821 gcatttttca ccggatcggc gcggaaatca gcataacgaa cgtactgttc gttcttctcg
     8881 ctgcggcgca tttcgatcag tttattttgc tgccagaagg cattaaacat cggcatagtc
     8941 acgcgttgcg cacgagcacc tttctgagcg gcatcataga aaaatttcag ccacgccatt
     9001 tcatctttac cttcggtata gatctctttt ccgccaggtt tcagtaattc agcaagatcg
     9061 gcaaatacgt caaaatcgtt acgcgcttca aattgcggag caacagcctg cttcatcggc
     9121 acaatatgct ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg
     9181 gtgatcggta atacgatatc tgcatgttta gcagctgcgg tccagtagca ttcagaaacg
     9241 acgatcatct ccggtttctg ccatgctttg atcagacgat tagtatcctg gtgatgggta
     9301 aagttaccgc cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg
     9361 ttatgctgat atttgccacc aggattttcc agcgcatcga cgatacgggc aacaggaatc
     9421 gccgtcattc cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt
     9481 tccggcagca cgccgccaac acgcgtcgga ttgccaccgt tggagtaatg ataagagaaa
     9541 ccaaaaccgc cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc
     9601 cagtgtttct gttcaccata ttgctggcgc tgaattcccc agccagccat cagcattgtg
     9661 cgattcgccg ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt
     9721 tccgccgccc aggccgcgct cttcggagta ttatcgctct tacctgtcag atactcttca
     9781 aactgcggat acccggtggt gtatttttca aggaacactt tatcgtgttt accttgtgtc
     9841 atcagggtat gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc
     9901 caggtggcat tatcaccaaa gaactcgatg gtttcggagc ggataggatc aatggcaatc
     9961 actggtttgc cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg
    10021 ctccaggcaa tttttaaggt attcagcggg ttcatccccc acagcaccac agcctggctg
    10081 ttttccagaa tcagcggcca ggaggtctgc tgttcataca cctctacaga accgaccaca
    10141 tgcggcatga tcacctgtgc tgccccggtt gaataatcgc cgctatgccc ggaataaccg
    10201 cccgccaggt tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag
    10261 cgccagccgt atgatccggc aaaaatagcc gatggtccgt tagctttgcg aatgcgatcg
    10321 tgttgctcat gaatcagttt taatgcttgc tcccagctca cctgtacata agtatcttcg
    10381 ccacgacctt tcgccggttg cagtggatta tcgagatagc tttttcttac catcggatgc
    10441 tgaatacgtg ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg
    10501 gttttcgcca gcgcgcctgt cgaagaaaca atcttgccgt ccttcacttc tacgctcatc
    10561 gctccccaac ggcccgcggt gaggatttta ccgcccttct cttctgccca tgcgggtagc
    10621 ggtgctgccg atgtcaccac cagcgcccca gcggcaatac cgctgtgttt aataaattca
    10681 cgtcttgtta atgtcataac ttcctccctg atcaacgagg atcactgttt ctcggtaata
    10741 tctttggcgt tgtactggaa gtaccgcgtc aaaatgtcca gttcgttttc gctcatgctg
    10801 gttcgcgcac ccattccttt ggcaatggac ggccatgcat tgacggtgta atggtcagcg
    10861 gcaataggcg catggcaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat
    10921 agcggtttac ggtccgctaa tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc
    10981 cattgattgc cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct
    11041 tcttcactta atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca
    11101 ctgccctgca tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg
    11161 acggtgagat cggcaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa
    11221 ggataaatat gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc
    11281 gcgttgtcat ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata
    11341 caggtttcgc cgcccttttg tgctttgtta tgcattttct gcgcagattc actttgcgag
    11401 gcaatatcca tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt
    11461 aattctttcc atactgtttc ggccatttcc tggcgatgag tttcgaactt atcgtcactg
    11521 tctattttgc cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat
    11581 aaataatcca tccctgactt tggaatatgg caatcggcac attccgcacg tatccctttc
    11641 tggttcgaaa agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag
    11701 gaaaggcaaa atgctgtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg
    11761 ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc
    11821 atagattcct ctaaataaat tccagaacac cgaaatcatt gtataattaa atacacaacg
    11881 aattaaccac ttgcataact cggcacaaca ctgcctgaaa taatcgataa agaatatgat
    11941 ttattacaat gtaatcatta attgctaagg aataacccag ttgctattta gaatatatat
    12001 ttatcagttt tagcaattta aatcccataa tcaatgtgaa tataaaaaat attattagca
    12061 caacgctatg ttcactgtca cccgctcaac agatcacggt tatcgttcgt tttttatact
    12121 gctcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca
    12181 tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct
    12241 ttcatttcag caaccgccgc cccgtctacg atataacgcg aatactcgtc cgcgtgttca
    12301 tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc
    12361 gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg
    12421 actcctgctc cggccataat gattggagca tcacgatggg caataagttc cataattttt
    12481 gataaacctt gcagtgagtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat
    12541 tccgcaagat tattgagtgt atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa
    12601 gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc
    12661 ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc
    12721 acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg
    12781 cgaattatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag tacccccaac
    12841 gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt
    12901 tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa tttccagtaa tgccattttt
    12961 gctccttaat tacgctgact gctcgctggc aacgatctct tcaatggacc acggatgaaa
    13021 cttaatagtt gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc
    13081 accttttggt gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag
    13141 caaagccagc gcacgggcat tcagcgtttc tggatcaccc ggcagaacga tttcaatatg
    13201 ttcccaacct tcgtgtgggt aacgtttttc cccgggccac ggtagctcca caatagaaaa
    13261 ctgccagtgc gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt
    13321 gatcatattt tctgacaaaa gctcgccaca ctgttcaaac ccgcgacgcc agcgctcagc
    13381 ggtgacgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatattcaa
    13441 ccccagacgg cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat
    13501 atcctgcagc tcatcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa
    13561 agcgttaatt taccctgttg ccctgtgtca accaaccgct gatttcacgc cgcttctgat
    13621 gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag
    13681 tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt
    13741 aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg
    13801 cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca gtcagcaaaa gttcagttcg
    13861 gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac
    13921 aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga
    13981 tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc
    14041 agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg
    14101 ttgactactc tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca
    14161 ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa
    14221 accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc
    14281 agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga
    14341 aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac
    14401 tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga
    14461 cgcagaacca gatcacctac gatcgtctca acgtgacgct gacccgtgat gacgtgatgg
    14521 gcgaaagcct ctacaacccg atgctgccag gaattgtggc ggatctcaaa gccaaaggtc
    14581 tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa aacaaggaag
    14641 gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg
    14701 atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctatattaca
    14761 tcgactcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcaggct
    14821 atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg
    14881 gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact ggccgatctg ctggatgaag
    14941 ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc ggatatgcca gctgacgagc
    15001 tggaaaaact ggctaatgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa
    15061 accgcaccac agactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg
    15121 cgccatacat gcagtatgct tacacgcgtg tattgtccgt gttccgtaag gcagaaatta
    15181 acgaagagca actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg
    15241 cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc
    15301 atgtgatgtg tgcttacctg tacgatctgg ctggtctgtt ctctggcttc tacgagcact
    15361 gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac
    15421 tgacagcgaa gacgctaaag ctgggtctgg atacgctggg tattgagaca g
//
LOCUS       DAFAVF010000101        15441 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFAVF010000101 DAFAVF010000000
VERSION     DAFAVF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.101"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(72..1052)
                     /locus_tag="LGK42_003060"
     CDS             complement(72..1052)
                     /locus_tag="LGK42_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBK2659286.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1060..1770)
                     /locus_tag="LGK42_003061"
     CDS             complement(1060..1770)
                     /locus_tag="LGK42_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBK2659287.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSSVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1987..3810)
                     /gene="typA"
                     /locus_tag="LGK42_003062"
     CDS             complement(1987..3810)
                     /gene="typA"
                     /locus_tag="LGK42_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBK2659288.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4183..5592
                     /gene="glnA"
                     /locus_tag="LGK42_003063"
     CDS             4183..5592
                     /gene="glnA"
                     /locus_tag="LGK42_003063"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBK2659289.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5911..6960
                     /gene="glnL"
                     /locus_tag="LGK42_003064"
     CDS             5911..6960
                     /gene="glnL"
                     /locus_tag="LGK42_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBK2659290.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6972..8381
                     /gene="glnG"
                     /locus_tag="LGK42_003065"
     CDS             6972..8381
                     /gene="glnG"
                     /locus_tag="LGK42_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBK2659291.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8493..8603
                     /locus_tag="LGK42_003066"
     CDS             8493..8603
                     /locus_tag="LGK42_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBK2659292.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8793..10166)
                     /gene="hemN"
                     /locus_tag="LGK42_003067"
     CDS             complement(8793..10166)
                     /gene="hemN"
                     /locus_tag="LGK42_003067"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK2659293.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10355..10864)
                     /gene="yihI"
                     /locus_tag="LGK42_003068"
     CDS             complement(10355..10864)
                     /gene="yihI"
                     /locus_tag="LGK42_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBK2659294.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11446..12078
                     /locus_tag="LGK42_003069"
     CDS             11446..12078
                     /locus_tag="LGK42_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK2659295.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12460..15246)
                     /gene="polA"
                     /locus_tag="LGK42_003070"
     CDS             complement(12460..15246)
                     /gene="polA"
                     /locus_tag="LGK42_003070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK2659296.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         3628 a         4003 c         3859 g         3951 t
ORIGIN      
        1 gttttaaagg caataagata ccgaccattc atatggaatg gttttattaa ctgcgtatga
       61 gttaaaactt attagtggtt cagaagatgg tttgcctctt gtttgatttg ttgtagtacg
      121 ttacgcacat gatttatctg attaattgca tcttcttttt ctttacgtaa ccgcgcatcc
      181 agtaaatgat tttctggcaa tggtgtttca ctcatctggc gataagggat ccacggatta
      241 tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat caacctcttc ttccaggcca
      301 tatgccgtca cctgcggctc atcatcaccc aggcatataa ggtgcgttaa taacgctttg
      361 aacggcatat caccctgacc tgaaatacag gctttatgac ccaggccacc cggttcttta
      421 acgatcaagg catctttgat atggacctgg gtaatatgcg gcgccatcgt ttttaaagcg
      481 tcgatgggat gctcatttgc attgatcatg ttcgcaaaat caaacagtaa ggaaagagat
      541 tccatctcac tttctttgac cagcgacacc agctcatggc tttttaaatc ttcatgctgt
      601 tcgatcgtaa aagtcaggcc gctgtcctga tacgtttccc gtacataggc aatgtcgtta
      661 gcgatgatcg ataatacgtc gcgcaaatta ccttcataac gtggataaaa acgtacggac
      721 gatgccccag ttttcaacgc aatggcgacg gcttcgtcaa tagatgcctt atctgaggcg
      781 ctggtttcaa tatgaatatc aaggttcaga cggcgggctt tatctccaaa ggcagagagt
      841 tctttatcgt ccatattacc aagagaaaaa cgctcgccat ccagaacatg gattttgacg
      901 ccacgtagat tattttcact tgcgatatca agtaaatcag caggcaacac tctttccagc
      961 cgcatattta aatgaaaggc ataagcatgt aaataaagag gaagagtgtc gacacgttgt
     1021 agaatctttc ttgcgttatt tattgtcacc ataaaacacc tacagattaa tgatgaggtc
     1081 tacggcctca aatcgccaat attcaatatc tatctggaca atattgcctt gctggtcggc
     1141 gcgatgcttt tcaattaaga tcgctggcat acccgcagaa ccgccaagat acttgctgat
     1201 atcgcccggc attctgacgg gtttgaaaat caattttttt actaccgtct ctttttggta
     1261 gcacttttcc cagactgaag aaaatgagtg gttttcaagt tgttcaataa aacccggagc
     1321 aacttcagga ttaatatatg tttcgtgata gaaaacttta tgtccttcca gcgcccccca
     1381 gccggtgatg cgatagagtt cagttgatgg cgtcactgca atcagtggcg cgagcgtctc
     1441 gggaatatcg ctggtacggc ttttctcggt aaacccccag gaaggttccc gcccttgctc
     1501 aattgcggcc cgctgaaagc tcgccgacag ctccgggctg taattaaatc gcggctgagt
     1561 cacgaaccac cctttacgat ccttacggaa gattttggat tctgcttcga ggttcagcaa
     1621 cgcctggcgc agcgtcatac gtttaatgcc cagcagctct cccagctctc tttctgaagg
     1681 gagttttcca cccggcgtag tgatgccatc ttttaaccac cgatccagtt tctcttttgc
     1741 attttctacg gtggattgat ttcccgtcat tgtttaatcc ttttggttta aaccagatga
     1801 ggcatcttac gcttacgcca aaaaagaaag caattaacgg cgtatgactt tgcagataat
     1861 tgtgacaaag ctctctattg acgtaatccc agacttataa gggagatact cgcattggtg
     1921 tgcgctcacg acaggtaaaa aaaaacctgc cagcgatggc aggtttttta taactaaaaa
     1981 acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga cgatcgtttt ccgtcaggtg
     2041 acgtttacga atacggatag aggtcggagt cacttctacc agttcgtcat catcgatgaa
     2101 ctccagagct tgttccagag tcatgcggat aggcggaacc agaacaacgg cttcgtcagt
     2161 accggaagca cgcatgttgg tcagtttctt accggtcagg cagtttacag tcaggtcgtt
     2221 agagcggcta tgaataccga taatctgacc ttcgtaaact tctgcaccgt gaccgaggaa
     2281 cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg accgctttac cctgaccgtt
     2341 agagatcagt acgccgttct gacgctgacc cacttcaccc ggacgtacgt cgtcgtagtg
     2401 gctgaaggtg gagtacagca gaccagtacc ggaagtcatg gtcatgaact cagaacggaa
     2461 gccaatcaga ccacggcttg ggatcacgta gtcgagacgt acgcggcctt taccgtctgg
     2521 attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc tgcattacag aaccctgatg
     2581 ctgttcttca acgtccagag tcacgttttc atatggctct tgtttacgac cgtcgatttc
     2641 acggaagata actttcggac gggataccgc cagttcgaaa ccttcacgac gcatgttttc
     2701 gatcagaaca gacaggtgta gttcgccacg accagaaacg cggaacgcat cagcgtcttc
     2761 ggtttcttct acgcgcagcg caacgttgtg taccagttct ttgttcagac gatccaggat
     2821 ctgacgagac gttacgaact taccttcttt accgcagaac ggcgaggtgt taacgcagaa
     2881 gaacatagaa acggtcggct catcaacgga gagtgccggc agcgcttcaa cgttttgcgt
     2941 gtcgcaaacg gtgtcagaaa tgttcagttc gccaaggccc gtgatcgcaa cgatatcgcc
     3001 agcttccgcc aggtcggttt cgatacgttc cagaccgagg tggcccagca ctttaccgac
     3061 tttcgcgttg cgggttttgc cttcgctatc gatgatagtg acctgctggt tcggcttcac
     3121 tttaccgcgc ttgatgcggc caatgccgat aacgccaaca tagctgttgt aatcgagctg
     3181 agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc ggcgcaggaa cgtggtcaac
     3241 aatcgcctgg tacagcgggg tcatgtcttc cgccatatct tcgtggtcca gacccgcgat
     3301 accgttcagc gcagaagcgt aaacgatcgg gaagtccagc tgctcgtcgg tcgcgtcgag
     3361 gttaacgaac agatcgaata cctgatccac aacccaatca ggacgcgcgc cagggcggtc
     3421 aactttgttg ataacaacaa tcggcttcag gccgtaagca aacgcttttt tggttacgaa
     3481 gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc agcagcactg agtctaccat
     3541 ggacattaca cgttcaactt caccaccgaa gtcggcgtgc cccggggtat caacgatgtt
     3601 gatacggtaa tcattccatt tgatagcggt gtttttcgcg aggatggtaa tcccacgctc
     3661 tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt tcggcacgag agtcgaacgt
     3721 accggattgt tggagcagct tgtctaccag ggtggtttta ccatggtcta cgtgcgcgat
     3781 gatggcgata ttacgcaatt tttcgatcac aactttgcct caggcattag aaatagcgcg
     3841 ttattgtaca cggattaatc gcactacaaa acaggatcac aaacatcctc cgcaaacaag
     3901 tattgcagag tccctttgtg atcgctttca cggagcataa aaagggttat ccaaaggtca
     3961 ttgcaccaac atggtgctta atgtttccat tgaagcacta tattggtgca acattcacat
     4021 cgtggtgcag cccttttgca cgatggtgcg catgataacg ccttttaggg gcaatttaaa
     4081 agttggcaca gatttcgctt tatctttttt acggcgacac ggccaaaata attgcagatt
     4141 tcgttaccac gacgaccatg accaatccag gagagttaaa gtatgtccgc tgaacacgta
     4201 ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt tgcgcttcac cgatactaaa
     4261 ggtaaagaac agcacgtcac tatccctgct catcaggtga atgctgaatt cttcgaagaa
     4321 ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag gcattaacga gtccgacatg
     4381 gtgctgatgc cagacgcatc caccgcagtg attgacccgt tcttcgccga ctccaccctg
     4441 attatccgtt gcgacatcct tgaacctggc accctgcaag gctatgaccg tgacccgcgc
     4501 tccattgcga agcgcgccga agattacctg cgttccactg gcattgccga caccgtactg
     4561 ttcgggccag aacctgaatt cttcctgttc gatgacatcc gtttcggatc atctatctcc
     4621 ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga actcctccac ccaatacgaa
     4681 ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt acttcccggt tccaccggta
     4741 gactcggctc aggatattcg ttctgaaatg tgtctggtga tggaacagat gggtctggtg
     4801 gttgaagccc atcaccacga agtagcgact gctggtcaga acgaagtggc tacccgcttc
     4861 aataccatga ccaaaaaagc tgacgaaatt cagatctaca aatatgttgt gcacaacgta
     4921 gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac cgatgttcgg tgataacggc
     4981 tccggtatgc actgccacat gtctctgtct aaaaacggcg ttaacctgtt cgcaggcgac
     5041 aaatacgcag gtctgtctga gcaggcgctg tattacattg gcggcgtaat caaacacgct
     5101 aaagcgatta acgccctggc aaacccgacc accaactctt ataagcgtct ggtcccgggc
     5161 tatgaagcac cggtaatgct ggcttactct gcgcgtaacc gttctgcgtc tatccgtatt
     5221 ccggtggttt cttctccgaa agcacgtcgt atcgaagtac gtttcccgga tccggcagct
     5281 aacccgtacc tgtgctttgc tgccctgctg atggccggtc ttgatggtat caagaacaag
     5341 atccatccgg gcgaagccat ggacaaaaac ctgtatgacc tgccgccaga agaagcgaaa
     5401 gagatcccac aggttgcagg ctctctggaa gaagcactga acgaactgga tctggaccgc
     5461 gagttcctga aagccggtgg cgtgttcact gacgaagcaa ttgatgcgta catcgctctg
     5521 cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc cggtagagtt tgagctgtac
     5581 tacagcgtct aatagttgaa gttgtactac ccggcgcaac aacgccggag gttcaaacca
     5641 ggcccatctg gcgtaattgt tgcagccagt ttgaacacgg acagcgcgca gaacccggag
     5701 cgtacactgg tacgtgagga gttcgagcac tgcccaggtt caaaatggca aataaaatag
     5761 cctgatggga ctggttttta gttgccgtgg aaatttcagc ccatctctgc atgggctttt
     5821 ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat aatgcactaa
     5881 aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc cgatgctggg
     5941 cagatcctca actcgctgat taacagtatt ttgttaatcg atgacaacct ggcgatccat
     6001 tacgccaacc ctgccgcgca acaactgctc gcccaaagct cccgcaaatt gtttggtaca
     6061 ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagtctg
     6121 gaggcggggc aaggttttac cgataacgaa gtgacgctgg tcatcgacgg gcgctcgcat
     6181 atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga gatggctccg
     6241 atggataacc agcgccgctt aagtcaggaa cagctacagc acgcccagca ggttgctgcc
     6301 cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg tttacgtggc
     6361 gcggcgcagc tgctcagcaa agcgttacct gacccatcac tactcgaata taccaaagtg
     6421 attatcgaac aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg gccgcagctg
     6481 cccggtacgc gcgttaccga aagtattcac aaagtggctg aacgcgtggt aacgctggtg
     6541 tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag cctgccggaa
     6601 ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta
     6661 caggcgctgg ggccggaagg cggtgaaatc attctgcgta cccgcaccgc gtttcaactg
     6721 accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga taacgggccg
     6781 ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg ccgcgaaggt
     6841 ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc aggcaaaatt
     6901 gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat caggaaataa
     6961 aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt
     7021 gggtgcttga acgtgcgctc gctggggcag gtttaacctg tacgacgttt gagaacggcg
     7081 cagaagtgct ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca gatatccgta
     7141 tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat ccaatgcttc
     7201 cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc tatcaacaag
     7261 gggcgtttga ttatctgccc aaaccgtttg atatcgacga agcagtggcg ctggttgagc
     7321 gcgctatcag tcattaccag gaacagcagc agccgcgtaa tgttcagctt aacggcccaa
     7381 cgaccgatat catcggcgaa gcgccagcca tgcaggacgt gttccgtatt atcggtcggc
     7441 tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt aaagaactgg
     7501 tcgctcatgc cctgcatcgc cacagtccgc gcgccaaagc gccgtttatc gcgctgaata
     7561 tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag aaaggcgcgt
     7621 ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc ggtacattat
     7681 tcctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg cgcgtgctgg
     7741 cagacggtca gttttaccgc gttggcggct atgcgccggt gaaagtggat gtgcggatta
     7801 tcgctgccac tcaccagaat ctcgaacagc gagtgcagga aggtaagttc cgtgaggatc
     7861 tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa cgtcgggaag
     7921 atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg ggcgtagaag
     7981 cgaagttact gcatccggaa accgaagctg ctctgacgcg tctggcgtgg ccaggcaacg
     8041 tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg caggaagtgt
     8101 tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt acttcgcaaa
     8161 tgcaaccgga cagctgggcg acgcttcttg cgcagtgggc agacagagcg ctgcgttccg
     8221 gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta ctgacgaccg
     8281 cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc tggggccgca
     8341 acaccctgac gcgtaagtta aaagagctgg ggatggagtg attcacagct tgtgtgtaaa
     8401 gattgattat tgagcgcaaa ttgctggtat tttacgcttt actgttccga taagttcagt
     8461 atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt aatctggtat
     8521 cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg ctgtgtgccg
     8581 caatgattgg gctgtttagt tgagtttcag attgctgatg aagtgctctt tgttcatgcc
     8641 ggatgcggcg tgaacgcctt atccggccta caaaagcaat attttcaaca ggttttgtag
     8701 gcctgataag cgtagcgcat caggcaatct tgttctcagc acattgatgg cgctttcgtt
     8761 ttctactttg taaacgaagc gccattcact atttaaatca cccgtgagaa ctgctgcatc
     8821 cgcgctttct ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga
     8881 cctttcgccg tcacctgtat tcccttctca tccacatcca ccagcccatc ttttgctaac
     8941 ggggcgagca gcttgagatc ttccgcaaag taatcagcga agtgcaaatc ccactgtttc
     9001 tcaataggag cgtaatccag acggaagttg cagatgagcg acttaatcac atcgcggcga
     9061 atacagtcat cacgcgttag cgcaatacca cgccacagcg cattgccttg ttcatccact
     9121 tgctgatagt actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg
     9181 atggcggaaa cgcccatccc cagcagatcg gtatcgccct gagtggtgta gccctggaag
     9241 ttacgatgca gcacgccttc acgctgggcc accgccagct cgtcatccgg acgggcaaag
     9301 tgatccatac cgataaactg atagcccgat tgcgtcagga aggcgatggt ttcctgcagg
     9361 atatcgagtt tttgctgcgg actcggcagg tcagcatctt tgattttgcg ctgagcagca
     9421 aaaatggtcg gcagatgcgc gtagttaaag acactcagac gatcggggtt cagctccgcc
     9481 acacgtttca gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg
     9541 tcgatgttgg tggaggtaaa accaatctca cgcgcatggt taagcagtgc aaagatgaac
     9601 tcttcatcct gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc
     9661 atgctcaggc gattaaagcc ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc
     9721 cgcggatcga cttcgatcga aatctccgca tcggcattga actggaagtt ttcgcgcagc
     9781 agcttcatca ggcggctgat ttgcgcttta ttcagatacg tcggcgttcc gccgccccag
     9841 tgcaattggc tgacgtgacg cccggcgaac agcggtgcac gatggacgat ttcttgctcc
     9901 agcgcgtcca gatactgatc ggccttgtgc tgctggcgag taacaatctt attgcaaccg
     9961 cagaagtaac aaagcttatg gcagaacggg atatgtacgt agagagataa tggacgctca
    10021 ggatagcgcg ccacggcttg taaaaacgcc tgttcgccga agtcttctga aaactccagc
    10081 gcggtcgggt acgaggtgta tcgtggcccg gaatagttat atttctggat cagggccaga
    10141 tcccagtcga tttgctgtac agacatgctc actccttccg atggcgtctc tggcggctac
    10201 ggcgtaccgg cggatgcccg tttcgggtca tcagccggtt gcgcagccac ttttgtcgcc
    10261 gcgacaaccg ctgtagttta acgaataacc acaccagata acatataacc ggaagggtta
    10321 tcagcagaac gggaaggccc acggcggcaa tccgttagtt gccccgcagc agacgcatca
    10381 tgtcttcttg cttctcgtct tcttcctctt cttcgtcatc gtcataagag aggccgagtt
    10441 tctgcatcaa ctcatcaata cgatccagtt tagcatccac ccaggattgc tcttcggcac
    10501 tcagggtttc gcctgcttcc agacgttcca gcagcgcatc cagacgctca tccgtttcca
    10561 gtaattccaa ctccgcctgc ggtgaaagca taggtttctc actcttcggt ttgtgctgtt
    10621 tggtgacttt ttcagtcacg cccaatggaa taggggtttt actgccaata cgtggatctt
    10681 ttggtgcgtt ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg cggctgcccg
    10741 gcgcatgacc acgacgtttt ttctgacgct tgcgatcacg agcttcctga tccagctcct
    10801 cgcgtgtttt tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt
    10861 tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag
    10921 aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac
    10981 ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc ctttagccgg
    11041 aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg tcttcctgac
    11101 aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta
    11161 gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa tcaaccgcca
    11221 tcagtatttg cacattttca gagttttata taaaagcaat tcactgattt atatagctta
    11281 tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg gcaatgtctc
    11341 acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt ttattatggt
    11401 tcaggtataa tcgccacaaa gctttgactg acggagacga ccgctttgac taatttgaat
    11461 tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc ttccgatacc
    11521 ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc gctgaacacg
    11581 ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac ccagcttatc
    11641 aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta cggttatgcg
    11701 gaagtcccgg aagagatgaa gcgcaaatgg cagcgtgcgc tcggcgaata cctcgaaaaa
    11761 cgtcagagcc tgcaaggtct ggtggtgcta atggatattc gccatccgct gaaagatttg
    11821 gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgttctggt gctgctgacc
    11881 aaagcggaca aactggcaag cggcgcacgt aaagcgcaat tgaatatggt gcgtgaagct
    11941 gtactggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgttgaa gaaacaaggc
    12001 gtggacaagc tgcggcagaa actggatacc tggtttagcg agatgcagcc tgtagaagaa
    12061 acgcaggacg gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc
    12121 ccgtaaattc aatagattac aattctaccg ttggcctgat aaacatagcg catcaggcat
    12181 tacggatctt ttctttcgcc caataaaaaa cgccccagtc attactgact ggggcggcta
    12241 aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga acatcttacc
    12301 tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc actttttgta
    12361 attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt gacagcttat
    12421 gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct gatcccagtt
    12481 ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt tttccatcag
    12541 ttgatgaatc tgcttcgcga cggcatcaac atcatcttta tgaacttcaa ataccagttc
    12601 atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc acgcatcaac
    12661 ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg cgttaatggc
    12721 tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg gcagatacag
    12781 acggcgtccg tccagcgttt caacgtagcc ctgctctttc gcctgagcac gggtgcgttc
    12841 catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc
    12901 tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat aaatcagacc
    12961 aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca gtggcaaacc
    13021 aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg cggtcagcaa
    13081 gcctttgtca cgcgagagat gcgccataat gcgcagttca atctgcgagt agtccgctga
    13141 gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac cttcttcgtt
    13201 acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg ttgcagttac
    13261 tgcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca gcggcagctt
    13321 gtcggtgtag gtcgatttca gcttcgccag accacgatac tccagaatca cttttggcaa
    13381 cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg cgccacccgg
    13441 cgttttcttc agcggtttaa taccctgttt ttcaaacaga atggtttgca actgcttggt
    13501 ggaagaaagg ttaaattctt cacctgcaat ttcatgcgct ttcttttcca gctcagccag
    13561 acgcaaggtg agctcttcag aatgattgtg cagcactttc ggatcgatct tcacaccgtt
    13621 acgttcaatg cgtgaaagca ccggcaccag cggcatttcg atattctcga agacgttcaa
    13681 cggccctttg tgtttttgca ggtccggcca cattttcaga tgcaactgca aggtgacatc
    13741 agcatcttcg gcggcgtaac gtccggcctc ttcgagggca atctggttaa aggtcagttg
    13801 atttttgcct ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca accaacgttc
    13861 cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg actccagcat
    13921 ggtatcaaac gcaatcccac gcagctcaat gccgtaattc gccagaatac cgcgatcgta
    13981 tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc
    14041 gagtgcacgc tcgcgagaga tttgatcggg cgcatcaaga taatcatgag caaccggaat
    14101 atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag cagagatgtt
    14161 atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca gcttcgcaat
    14221 ccacgctttc agtgtttctt catcaaggat ggtgacgtag ttgtcataag aaatcaccgt
    14281 tgccgtcact tctggcgctt cgtctgcaac actggtttcc tgcggcttcg cggctggttt
    14341 tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc gtttgaactc
    14401 atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt ccagttgttc
    14461 acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg agagataagc
    14521 aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga agctcaaccc
    14581 agcaattttt tctggctcgg catacagcgt atccagcccg ccaagacctt gcagcaatgc
    14641 ctgcgcggtt ttttcaccga cgcccggtac gccaggaatg ttatcggagg agtcacccat
    14701 cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca ccacctcttc
    14761 cggtccgagg atggtattcg tcatggtgtt gataagcgta atatttggcg tcaccagctg
    14821 cgccatatct ttatcgccag tgctgatcag caccggacgc ccggcttttt cggcttcgcg
    14881 cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca gcggcagtcc
    14941 catcgcttta accatcgcgt gcaggggttc gatttgtgct cgcagatcgt ccggcattgg
    15001 cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc ccttggcgtc
    15061 aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca gcatgttgag
    15121 gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg gaaacgcgtg
    15181 atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt gggggatctg
    15241 aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca gacaggatga
    15301 aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg gatcgctcgc
    15361 aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa tttattatca
    15421 tgctttttac agcatgatgt t
//
LOCUS       DAFAVF010000102        15339 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFAVF010000102 DAFAVF010000000
VERSION     DAFAVF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.102"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(95..2086)
                     /gene="tkt"
                     /locus_tag="LGK42_003071"
     CDS             complement(95..2086)
                     /gene="tkt"
                     /locus_tag="LGK42_003071"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK2659297.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2364..3122
                     /gene="loiP"
                     /locus_tag="LGK42_003072"
                     /gene_synonym="yggG"
     CDS             2364..3122
                     /gene="loiP"
                     /locus_tag="LGK42_003072"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBK2659298.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3328..4248)
                     /gene="speB"
                     /locus_tag="LGK42_003073"
     CDS             complement(3328..4248)
                     /gene="speB"
                     /locus_tag="LGK42_003073"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBK2659299.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4384..5115)
                     /locus_tag="LGK42_003074"
     CDS             complement(4384..5115)
                     /locus_tag="LGK42_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659300.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5261..7237)
                     /gene="speA"
                     /locus_tag="LGK42_003075"
     CDS             complement(5261..7237)
                     /gene="speA"
                     /locus_tag="LGK42_003075"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBK2659301.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7246..7377)
                     /gene="yqgB"
                     /locus_tag="LGK42_003076"
     CDS             complement(7246..7377)
                     /gene="yqgB"
                     /locus_tag="LGK42_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBK2659302.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7513..7728
                     /locus_tag="LGK42_003077"
     CDS             7513..7728
                     /locus_tag="LGK42_003077"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659303.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8032..9186
                     /gene="metK"
                     /locus_tag="LGK42_003078"
     CDS             8032..9186
                     /gene="metK"
                     /locus_tag="LGK42_003078"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK2659304.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9622..11016
                     /gene="galP"
                     /locus_tag="LGK42_003079"
     CDS             9622..11016
                     /gene="galP"
                     /locus_tag="LGK42_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBK2659305.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11093..11590
                     /locus_tag="LGK42_003080"
     CDS             11093..11590
                     /locus_tag="LGK42_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBK2659306.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            11685..12392
                     /gene="endA"
                     /locus_tag="LGK42_003081"
     CDS             11685..12392
                     /gene="endA"
                     /locus_tag="LGK42_003081"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBK2659307.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12472..13203
                     /gene="rsmE"
                     /locus_tag="LGK42_003082"
     CDS             12472..13203
                     /gene="rsmE"
                     /locus_tag="LGK42_003082"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK2659308.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13216..14166
                     /gene="gshB"
                     /locus_tag="LGK42_003083"
     CDS             13216..14166
                     /gene="gshB"
                     /locus_tag="LGK42_003083"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBK2659309.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14275..14838
                     /locus_tag="LGK42_003084"
     CDS             14275..14838
                     /locus_tag="LGK42_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBK2659310.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14838..15254
                     /gene="ruvX"
                     /locus_tag="LGK42_003085"
     CDS             14838..15254
                     /gene="ruvX"
                     /locus_tag="LGK42_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK2659311.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
BASE COUNT         3731 a         4060 c         3961 g         3587 t
ORIGIN      
        1 tctacaacac gatttatcta ttgcttgtcc gcaaacggac atatcaaggt aataaaaaag
       61 gtcgccgaag cgaccttttt acccgaaatg ctaattacag cagttctttt gctttcgcaa
      121 caacgttatc aacagtgaag ccgaactctt caaacagcag ctctgccgga gcagattcac
      181 cgaaggtggt cataccgacg atagcaccgt tcaggccaac atacttgtac cagtagtcag
      241 caatacccgc ttctacagca acgcgtgcag taaccgcttt cggcagtacg gattcacggt
      301 aagcagcatc ctgcttgtca aatgcgtcgg tagacggcat ggacaccacg cgcgctttca
      361 cgccttcggc agtcagtttt tcgtaggcag caacagccag ttcaacttct gaaccggtag
      421 cgatgaaaat cagttccggc tgaccggcgc agtctttcag cacataacca ccgcgcgcga
      481 tgtttgccag ttgctcttca gttcgttcct gctgcgccag gttctgacgg gagaggatca
      541 gtgcggtcgg gccgtcctga cgctcaacac cgtatttcca cgcgaccgcg gattcaacct
      601 ggtcacacgg acgccatgta gacatgttcg gggttacgcg cagagaagcg acctgctcta
      661 ccggctggtg agtcgggcca tcttcgccca gaccgatgga gtcgtgggtg taaaccatca
      721 cctgacgctg tttcatcagc gcagccatac gtacggcgtt acgtgcgtat tccacgaaca
      781 tcaggaaggt ggaggtgtac ggcaggaaac caccgtgcag ggagataccg ttagcaatcg
      841 cggtcatacc gaactcgcga acaccgtagt ggatgtagtt acccgcagca tcttcgttga
      901 ttgctttaga accagaccac agggtcaggt tagacggtgc caggtcagca gaaccgccga
      961 ggaattccgg caacagcgga ccgaaagctt cgatagcatt ctgagacgct ttacggctgg
     1021 cgattttcgc cgggttagcc tgcagtttag caatgaactc ttttgctttc gcgtcgaagt
     1081 cagacggcat ttcgcctttc atacggcggg taaattcagc ggcttcctgc ggataagctt
     1141 tcgcgtaagc agcgaatttc tcgttccatg cggattcttt cgcctggcct gcttctttcg
     1201 catcccactg agcatagatt tcagacggga tttcgaacgg tgcatatttc cagcccagct
     1261 gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc accgtgggag tcgtgagtac
     1321 cggctttgtt cggggaaccg aaaccgatga tggttttgca catcagcagg gacggtttgt
     1381 cagtcactgc gcgcgcttct tctactgcgc gtttgatgga tgccgcgtca tgaccgtcga
     1441 tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat tgcggtgtcg tcggtgaacc
     1501 agccttcaac gtgaccatca atagagatac cgttgtcatc gtagaacgca atcagtttac
     1561 ccagcttcag cgtacccgcc agagagcaaa cttcgtggga gatgccttcc atcatgcagc
     1621 cgtcgcccat gaaggcatag gtgtagtggt cgacaatgtc gtggcccgga cggttaaact
     1681 gcgccgccag cgttttttct gcaatcgcca taccgactgc gttggcaata ccctgaccca
     1741 gcggaccggt ggtggtttcc acaccagcgg tgtaacccac ttccgggtga cccggagttt
     1801 tagagtgcag ctgacggaag tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt
     1861 gcagcaggct gtagatcagc atggagccgt ggccgttgga cagcacgaag cggtcacggt
     1921 cagcccagga cggattctgc gggttgtgtt tcaggaaatc acgccacagg acttcggcaa
     1981 tgtcagccat acccataggg gcacccgggt gaccggattt ggctttctgt actgcgtcca
     2041 tgctcagcgc acgaatagca ttggcaagct ctttacgtga ggacattttg actccagatc
     2101 ggatgatgaa gggcacgccc ttaacgactt gacgacagcg cgttttgggc tacgccggaa
     2161 aatttgccaa caatttaccg caagccgcgc gtcatgtaca tggaacatcc ttttgccgct
     2221 tcagaaatct ctggatcatg ctcgcatgtt gcgcaatcta ctcgcccgtc cgctgcgctt
     2281 ttccttatac tgagactgag cgtcgattca cctgcaaacg gcgcattttt agaataatcc
     2341 tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg
     2401 caacggtact gactggttgc cagaatatgg actccaacgg actgctctca tcaggagcgg
     2461 aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat
     2521 gtcaggagat ggacagcaag gcgacgattg cgccagccaa tagcgaatac gctaaacgtc
     2581 tgacaactat tgccaatgcg ctaggcaaca atatcaacgg tcagccggta aattacaaag
     2641 tgtatatggc gaaggatgtg aacgcctttg caatggctaa cggctgtatc cgcgtctata
     2701 gcgggctgat ggatatgatg acggataacg aagtcgaagc ggtgatcggt cacgaaatgg
     2761 ggcacgtggc gttaggccat gtgaaaaaag gaatgcaggt ggcacttggt acaaatgccg
     2821 tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag tttatctcaa tcacaactcg
     2881 gtgatctggg cgagaaatta gttaattcgc aattctccca gcgccaggaa gcagaagccg
     2941 atgattattc ttacgatctt ctgcgccaac gcggcatcag cccggcaggt cttgccacca
     3001 gctttgaaaa actggcgaaa ctggaagaag gccgccaaag ctcaatgttt gacgaccatc
     3061 ctgcatccgc cgaacgcgcc cagcatattc gcgatcgtat gagcgcggat gggataaagt
     3121 aaagcctggt ggtgtcgaac gtaggtcaga taaggcgttc accccgcatc tgacatgaat
     3181 ggcacatttg ttaccttgtg cgcattgccg gatgcgatgc tggcgcatct tatccggcct
     3241 acgggtgccg aacgtaggtc ggataaggcg ttcacgccgc atccgacatt aatggcacgt
     3301 tttacccgtg cgcatcgcat ctggtgctta ctcgcccttt ttcgccgcct gaatatacag
     3361 catctccagc gccagcgtcg ccgcagccag agcagtgatt tccgactgat cgtatgccgg
     3421 agccacttcc actacgtcca tcccaacgat gttgagatct ttcaggccgc gtaccagttt
     3481 aatggcgcga tcggaggtca gaccgccaat taccggcgta ccggttcccg gcgcaaatgc
     3541 aggatccagg cagtcgatat caaaggtcag gtaaaccggc atatcaccca caatttgttt
     3601 cacctgggcg ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt
     3661 aaagccgttg tctttatcaa attcggtacg aataccaatc tgcacggaat gattcggatc
     3721 gatcagacct tctttcggcg cggtatagaa catggtgccg tggtcaaatt cacaaccgtt
     3781 cgcgtaggta tcggtgtggg cgtcaaagtg taccagcgcc attttgccga aatgcttcgc
     3841 atgagcacgc agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga gcatacgctt
     3901 accggcagcc agcagcttct cggcgtgcgc ctgcagcttc tcgctcatct cacgggcatc
     3961 gccgaaagca tataccagat caccgcagtc cacgacgttc agacgctcac gcatgtcgaa
     4021 attccacggg aagcggttgt gttcccaggc cagattcgtc gaaacctgac ggatagctgc
     4081 cggaccgtgg cgtccccccg cacgaccaga agtggccata tcgaacggca caccagtaat
     4141 cacccagtca gcatcgctgt catacggctg gaagttcatc ggcaggcgta aaaaaccaaa
     4201 ggcattggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg atggactcct
     4261 attacaaagt cgatataaaa aaacccttcc gcgtcgttag gcccgacgag gaagggttgg
     4321 atttgcgaaa cacaagtttg tgctaattat cgccttaaat gggcgaggca tcaagcgagg
     4381 ttgctatttc cgacacggtg gcggcgcata gccattgtta tctgccggaa aggcttgtcc
     4441 agagcacggc ggcagcgcgt tttgcgcctg gccttgtaat tcaaaggcgg caaagcctat
     4501 tacaccggta ttacgcacat cgccttgcgc cgaattggct gcgtaggaat cagcaacgtt
     4561 ggcgaactga aaagccgctt cggtatgttt atctttgcgg aatcctttaa tcgctaaaga
     4621 atctccggca ttcacaatat agccgttgtt attcagcgaa ccttgcttgc cgttcagcac
     4681 atccaggcca tcaacagtag caacgatctc gtagttggtg ttacgactgt aattacggac
     4741 atataattga taattcattc cgactctggc atggacaata tagcgtggtt cattattgga
     4801 tgaatcataa acacgcgtaa taggtagaga attaaaatta ccatcgcgaa tagcatattc
     4861 cagttcgtta atacgaatgc tataaacgcg gtcatagccg gaaggaacat tagtcgaata
     4921 actaataacg atcatctctg ccggatcgcg ccaggctcgc tcagcagata cgccctgaac
     4981 cgatgagtgc acatccccac cccaggcagt gccgacattg cccgatgcac tgctattata
     5041 ctgcgcatta ctggaacaac cggccagcag cacgattaat gccaccaacg cgctacgtac
     5101 tttccatttt ttcataaccg ttccctctgc atttaattaa tgcagcgatt gtagggagat
     5161 tatttaataa gaaaagcagc gagattattt ttaagaatat ataaaaagaa agcgggccga
     5221 tttacgtaaa taaatcgacc cgccagcgga ataatcttaa ttattcatct tcaagatagg
     5281 tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt tcagcatcaa
     5341 gatcggtttt cttcacttga tcgcggaact gggttaacag cgttttcgga tcgagctgta
     5401 catattgcag catgtccgcc acggtatcgc cttcgtcaga cagttctact tctacgctac
     5461 cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc gaacaggttg tgcatattgc
     5521 caaggatctc ctgatatgcg ccgaccataa agaaaccgag catcggcgga ttctctggat
     5581 cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcaatatag tggtcgatag
     5641 caccgtcaga gtcacaggta atatccagca gcacagcgcg acgttccggc acttgatcca
     5701 gcccttccag cggcagaacc gggaacaact ggtcgatccc ccatgcgtcc ggcattgact
     5761 ggaacagcga gaaattgacg tacattttgt ccgccatacg ttcctgcagt tcgtcgatta
     5821 tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg cacatgctca
     5881 gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa gagtagccga
     5941 tatgaatgtc gtgcagatcc atctgactgt cgtgtaacca ttcacgcaga gaacggcgag
     6001 ttcccggttc gtgcatctcc tgccaggttt cccacatgct ttgcagcgcg cgcggcgcat
     6061 cttctgcagg cgcggtcggc accgtgtatt cgttacgttc cacgccgatg atattagaca
     6121 ccagcacggt gtgatgcgca gtcaccgcac gacccgattc ggtgattacc gtcggatgcg
     6181 gcagaccgtt ttcttcacac gcatcaccaa tcgcccagat gatgttgttg gcatattcat
     6241 tgaggccgta gtttacggag cagtcagact gcgaacgggt gccttcatag tccacgccca
     6301 gaccgccgcc gacgtcgaag cactgaatat tgacgcccag cttgtgcagt tccacataga
     6361 aacgcgccga ttcacgaacg cctgtggcga tatcgcgaat attcgccatc tgcgaaccga
     6421 ggtggaagtg cagtagttgc aggctgtcga gacgcccggc ttcacgcagg gtttcaacca
     6481 gttgcagtac ctgagtcgca gccaggccga acttcgattt ttccccgccg gaggactgcc
     6541 atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga acgacattca
     6601 gacgttctgc ttcatccagc acaatggcga tttctgacat cttctcaatg accagataga
     6661 ccttgtgccc catcttctcg ccaattaatg ccaggcggat atattcgcgg tctttataac
     6721 cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc cagcactgcc atcaactcgg
     6781 ctttggaacc ggcttccaga cccagcggtt cgcccgaatg aatcagggac tcaatcacgc
     6841 gacggtgctg gttaactttg atcggataaa caaggaagta atcgccgtta tagccgtagg
     6901 attcccgcgc acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc aggatctgtg
     6961 ggaaacagaa cagtgcaggc agacgctggc cctgtgcttc acgagttttc actaactgcg
     7021 cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctgatg tggcccagct
     7081 cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc agcatcttgc
     7141 tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg tgttcgcccg
     7201 ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa ttattttatt
     7261 aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc gataacaacc cataagcaca
     7321 tggattttcc agcagtgaat gctgacgctc caactgcgca accggtttct ttttcataac
     7381 attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg agaaaactct
     7441 tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc ttgaatcctg
     7501 agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc agagtgcggg
     7561 gaacgaatct tcaccagaac ggtgagacag gttaagcagc agacaacggt tcattatttc
     7621 gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct gatttcaacc
     7681 cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc cgcgcgccgc
     7741 gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat gccggcattg
     7801 tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa aaaaagtgga
     7861 aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc ataccgatta
     7921 acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc taccacagaa
     7981 aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa tatggcaaaa
     8041 caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc tgaccaaatc
     8101 tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt tgcttgcgaa
     8161 acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag cgcctgggta
     8221 gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca ttccgacatg
     8281 ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc tcctgacatc
     8341 aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca gggtctgatg
     8401 tttggctacg caactaatga aaccgacgtg ctgatgccag cacctatcac ctatgcacac
     8461 cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg gctgcgcccg
     8521 gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg tatcgatgct
     8581 gtcgtgcttt ccactcagca ttctgaagag atcgaccaga aatcgctgca agaagcggta
     8641 atggaagaga tcatcaagcc aattctgccc gctgaatggc tgacttctgc caccaaattc
     8701 ttcatcaacc cgaccggtcg tttcgttatc ggtggcccaa tgggtgactg cggtctgact
     8761 ggtcgtaaaa ttatcgttga tacctacggc ggcatggcgc gtcacggtgg cggtgcattc
     8821 tctggtaaag atccatcaaa agtggaccgt tccgcagcct acgcagcacg ttatgtcgcg
     8881 aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc ctacgcaatc
     8941 ggcgtggctg aaccgacttc catcatggta gaaactttcg gtactgagaa agtgccttct
     9001 gaacaactga ctctgctggt acgtgagttc ttcgacctgc gcccatacgg tctgattcag
     9061 atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca ctttggtcgt
     9121 gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc tgccggtctg
     9181 aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc gctggccttt ttcttttacc
     9241 tcaccgcggt ggataggcat tcacgccgca tccggcaaaa agctgcccgc acaataacat
     9301 cattcttcct caacacgttt tatcgcaaat tatcatcact actgaaaccg attacaccaa
     9361 taacaccagc caaatatttg taatattttc atattattat tcagttttca cagttgttac
     9421 atttcttttc agtaaagtct taattgcaga taacagcgtt taatctatga tgatataact
     9481 caattatttt catgcactta aaccataact aagataaatg ttagtgtaag cgattacact
     9541 gatgtgattt gcttcacatc tttttacgtc gtactcacct atcttaattc acaataaaaa
     9601 ataaccatat tggagggcat catgcctgac gctaaaaaac aggggcggtc aaacaaggca
     9661 atgacgtttt tcgtctgctt ccttgccgct ctggcgggat tactctttgg cctggatatc
     9721 ggtgtaattg ctggcgcact gccgtttatt gcagatgaat tccagattac ttcgcatacg
     9781 caagaatggg tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt gggcagcggc
     9841 tggctctcct ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat tttgtttgtt
     9901 gccggttcgc tgttctctgc ggctgcgcca aacgttgaag tactgattct ttcccgcgtt
     9961 ctactggggc tggcggtggg tgtggcctct tataccgcac cgctgtacct ctctgaaatt
    10021 gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc agttgatgat cactatcggg
    10081 atcctcggtg cttatctttc tgataccgcc ttcagctaca ccggtgcatg gcgctggatg
    10141 ctgggtgtga ttatcatccc ggcaattttg ctgctgattg gtgtcttctt cctgccagac
    10201 agcccacgtt ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt gctgctacgc
    10261 ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg aaatccgtga aagtttgcag
    10321 gttaaacaga gtggctgggc gctgtttaaa gagaacagca acttccgccg cgcggtgttc
    10381 cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga tgaacgtcat catgtattac
    10441 gcgccgaaaa tcttcgaact ggcgggttat accaacacta ccgagcaaat gtgggggacc
    10501 gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg caatcggcct tgttgaccgc
    10561 tggggacgta aaccaacgct aacgctgggc ttcctggtga tggctgctgg catgggcgta
    10621 ctcggtacaa tgatgcatat cggtattcac tctccgtcag cgcagtattt cgccatcgcc
    10681 atgctgctga tgtttattgt cggttttgcc atgagtgccg gtccgctgat ttgggtactg
    10741 tgctccgaaa ttcagccgct gaaaggccgc gattttggca tcacctgctc caccgccacc
    10801 aactggattg ccaacatgat cgttggcgca acgttcctga ccatgctcaa cacgctgggt
    10861 aacgccaaca ccttctgggt gtatgcggct ctgaacgtac tgtttatcct gctgacattg
    10921 tggctggtac cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa
    10981 ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc ctcccatcgc
    11041 ggaggaagcc acctcttgca gtcatctttt cttcgctcta tcctctgccg ctatgaaaac
    11101 atcccgtctc cctatcgcca tccagcaggc cgttatgcgc cgcctgcggg aaaaactcgc
    11161 ccaggccaac ctgaagctag ggcgtaacta cccggagcca aaactctctt acacccagcg
    11221 cggaacctcc gccggaacag cctggctgga aagctatgaa atccgcctca atcccgtttt
    11281 gctgttggaa aacagtgaag cttttattga agaagtggta ccgcacgaac tggcacattt
    11341 gctggtatgg aaacatttcg gccgcgtagc gccacatggc aaagagtgga agtggatgat
    11401 ggaaagcgtg ctgggcgttc ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg
    11461 tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat cagcttaccg tacgccgcca
    11521 taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt cactgcggtg aacagctggt
    11581 tgcgaaataa ccatctgaac tatcaggaac tttcctgatc tggctgattg cataccaaaa
    11641 caactttcgc tacgttgctg gctcgtttta acacggagta agtgatgtac cgttatttgt
    11701 ctattgctgc ggtggtactg agcgcagcat tttccggccc ggcgttggcc gaaggtatca
    11761 atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca cgctgacgcg cccggtacgt
    11821 tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt
    11881 gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg cgtagagtgg gaacacgtcg
    11941 ttcccgcctg gcagttcggt caccagcgcc agtgctggca ggacggtgga cgtaaaaact
    12001 gcgctaaaga tccggtctat cgcaagatgg aaagcgatat gcataacctg cagccgtcag
    12061 tcggtgaggt gaatggcgat cgcggcaact ttatgtacag ccagtggaat ggcggcgaag
    12121 gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag
    12181 cgcgtgcacg cggtgccatt gcgcgcacct acttctatat gcgcgaccaa tacaacctga
    12241 cactctctcg ccagcaaacg cagctgttca acgcatggaa caagatgtat ccggttaccg
    12301 actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca aggcaatcat aacccgtatg
    12361 tgcaacgcgc ttgccaggcg cgaaagagct aacctacact agcgggactc tttttgttaa
    12421 cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc
    12481 ccccgcattt atcatcctga accactgacc agccattctc acatcgcgct ttgcgaagat
    12541 gccgccaacc atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt
    12601 gacggtagca accaggtctt tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa
    12661 gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc cgctgcatat tcacctcggt
    12721 caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc agaaatcgat cgaactcggt
    12781 gtaagcctca ttacgccact tttttctgag cgctgcggcg ttaaactgga tagtgaacgt
    12841 ctgaacaaga agctccagca gtggcagaag attgcaattg ctgcctgtga gcagtgtggt
    12901 cgtaaccggg tgccggaaat ccgcccagcg atggatctgg aagcctggtg tgcagagcag
    12961 gatgaaggac tgaaactgaa tcttcacccg cgcgccagta acagcatcaa tacgttgccg
    13021 ttaccggttg aacgcgtccg cctgctgatt ggcccggaag gcggtttatc ggcagatgaa
    13081 attgccatga ctgcccgcta tcaatttact gatatcctgt tgggacctcg cgttttgcgt
    13141 acagagacaa ctgcgctcac cgccattacc gcgctacaag tacgatttgg cgatttgggc
    13201 taacggagaa gaataatgat caagctcggc atcgtgatgg accccatcgc aaacatcaac
    13261 atcaagaaag attccagttt cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt
    13321 cactatatgg agatgggcga tctgtatctg atcaatggcg aagcccgcgc ccatacccgc
    13381 acgctgaacg tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga acaggatctg
    13441 ccgctggccg atctcgatgt gattctgatg cgtaaagacc cgccgtttga taccgagttt
    13501 atctacgcga cctatattct ggaacgtgcc gaagagaaag gaacgctgat cgttaacaag
    13561 ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg cctggttctc tgacttaacg
    13621 ccagaaacgc tggttacgcg caataaagcg cagctaaaag cgttctggga gaaacacagc
    13681 gacatcattc ttaagccgct ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa
    13741 ggcgatccaa acctcggcgt gattgccgaa accctgactg agcatggcac tcgctactgc
    13801 atggcgcaaa attacctgcc agccattaaa gatggcgaca aacgcgtgct ggtggtggat
    13861 ggcgagccgg taccgtactg cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat
    13921 ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc
    13981 cgtcagatcg gaccgacgtt gaaagaaaaa gggctgattt ttgttggtct ggatatcatc
    14041 ggcgaccgtc tgactgaaat taacgtcacc agcccaacct gtattcgtga gattgaagca
    14101 gagtttccgg tgtcgatcac cggaatgtta atggatgcca tcgaagcacg tttacagcag
    14161 cagtaaccca ccttagcgag aaggatctcg ttgagactct gagtgacagc gcccttcttt
    14221 ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa cagaacctct gacaatgaat
    14281 ttacagcatc actttcttat tgccatgcct gctctccagg atccgatttt ccgtcgttcc
    14341 gtggtctaca tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt caacaagccg
    14401 ctggaaaatc tcaaaattga agggattctg gaaaagctga agatcacgcc ggagccgcgt
    14461 gatgaatcaa tccgtctgga taaaccggtt atgctcggcg gtccgctggc tgaagatcgc
    14521 gggtttattt tgcatactcc gccctccaat tttgcttcca gcattcgcat ttcagacaac
    14581 acagtgatga ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct
    14641 gacgtattgg tggctctggg ttatgcctcc tgggaaaaag gtcaactgga acaagaaatt
    14701 ctcgataacg cgtggctaac ggccccggcg gatctgaata ttctgttcaa aacgccgatt
    14761 gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg atattctcac catgcctggt
    14821 gtggcagggc acgcctgatg agtggaacct tactcgcctt cgacttcggc accaaaagca
    14881 ttggcgtagc ggtcggccaa cgcattaccg gcaccgctcg ccctttgcct gcaattaaag
    14941 cacaggacgg tacgccggac tggaacatta tcgagcgttt actgaaagag tggcagccgg
    15001 acgaaatcat cgtcggtttg ccgctgaata tggacggcac cgagcaacca ttgactgcca
    15061 gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg tgttgaagta aagctccatg
    15121 acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt tgaacagggc ggctatcggg
    15181 cgctcaacaa aggcaaagtc gactctgcct ctgcggttat tattctcgaa agctatttcg
    15241 agcagggata ttaaggcgat ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg
    15301 atgcggcgtg aacgccttat ccagcacaca tctggcagc
//
LOCUS       DAFAVF010000103        15172 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFAVF010000103 DAFAVF010000000
VERSION     DAFAVF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.103"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(124..2325)
                     /gene="iutA"
                     /locus_tag="LGK42_003086"
     CDS             complement(124..2325)
                     /gene="iutA"
                     /locus_tag="LGK42_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBK2659312.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(2407..3684)
                     /gene="iucD"
                     /locus_tag="LGK42_003087"
     CDS             complement(2407..3684)
                     /gene="iucD"
                     /locus_tag="LGK42_003087"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBK2659313.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            complement(3681..5423)
                     /gene="iucC"
                     /locus_tag="LGK42_003088"
     CDS             complement(3681..5423)
                     /gene="iucC"
                     /locus_tag="LGK42_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBK2659314.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            complement(5423..6370)
                     /gene="iucB"
                     /locus_tag="LGK42_003089"
     CDS             complement(5423..6370)
                     /gene="iucB"
                     /locus_tag="LGK42_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBK2659315.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            complement(6371..8095)
                     /gene="iucA"
                     /locus_tag="LGK42_003090"
     CDS             complement(6371..8095)
                     /gene="iucA"
                     /locus_tag="LGK42_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBK2659316.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8231..9424
                     /locus_tag="LGK42_003091"
     CDS             8231..9424
                     /locus_tag="LGK42_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2659317.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            complement(9804..10184)
                     /gene="crcB"
                     /locus_tag="LGK42_003092"
     CDS             complement(9804..10184)
                     /gene="crcB"
                     /locus_tag="LGK42_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBK2659318.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(10525..10938)
                     /locus_tag="LGK42_003093"
                     /pseudo
     CDS             complement(10525..10938)
                     /locus_tag="LGK42_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            10936..11193
                     /locus_tag="LGK42_003094"
                     /pseudo
     CDS             10936..11193
                     /locus_tag="LGK42_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(11427..12284)
                     /gene="sitD"
                     /locus_tag="LGK42_003095"
     CDS             complement(11427..12284)
                     /gene="sitD"
                     /locus_tag="LGK42_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBK2659319.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(12281..13138)
                     /gene="sitC"
                     /locus_tag="LGK42_003096"
     CDS             complement(12281..13138)
                     /gene="sitC"
                     /locus_tag="LGK42_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBK2659320.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGVWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(13135..13962)
                     /gene="sitB"
                     /locus_tag="LGK42_003097"
     CDS             complement(13135..13962)
                     /gene="sitB"
                     /locus_tag="LGK42_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBK2659321.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(13962..14876)
                     /gene="sitA"
                     /locus_tag="LGK42_003098"
     CDS             complement(13962..14876)
                     /gene="sitA"
                     /locus_tag="LGK42_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBK2659322.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPINAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         3813 a         3904 c         3840 g         3615 t
ORIGIN      
        1 atctgtagtc agtaaagagt ctatcctccc tgactacaga ttatgtcgtg tattatatga
       61 tatcaacttg gctgtcagcg gccatgttga tatcagcgta cctttgttgt aaaggaatac
      121 cggtcagaac agcacagagt agttcagacc aaaggttcgg cccctgcctt tgtagtcgta
      181 cagtgacgct gggccgtaac ccgggctgta gtacagtggt gcacgctgcc cccagacagt
      241 ggtgtagtca cggtcgaaga ggttttcaat gctgaagctg agtgtaccca ccggaagctg
      301 ataactgccg agcagatcca cggtggtata gccatcgacc ttgtagccct gcgcgtcgct
      361 cacgtcaaag gaggtggtgc tctgcacgcg cagactccac gggtccggtg cccagccaat
      421 gtaggctgtc gcttttgatg ggcttgctgt cttcacatcg tatttctgcc aggtaccgtt
      481 cactttcgac tcagttttca gcacgttgaa gttcactccg gtactccagt cagtatcagg
      541 aatcaggtag tccaccgcac cttccacgcc gtaaatacgg cgtttgtcgt ccaccacgct
      601 gatggtcaga tctttattcg ccaccacgct cttatcagaa atcgaatagt aggccgcgat
      661 ttgggtacgc agattattgc cagtaaagcg ccagcccagc tcataagaat cgactttcac
      721 gccttccagc ttgctgtcgc tgacgttcac actctttgtt agaggaagat ggccgttcac
      781 tgcagcacca tagatgccgc gaccatagta tttacccggg tccggcagct ccacgccctg
      841 ggagaagttg agccatgcct gctggcgttc ggtgatgtgc atcagcagac cggcgttgaa
      901 caggaagttg tcgtaatcga ctgagccgcc aggaatggcg tcggcggatg tagccttccc
      961 ggcggcaatc tgccgttgct gcgcgtagcc gatgaaatca tcaatcttgt tttcagtgta
     1021 ctgatagcgt acgccaccgt tgagggtaaa gagattattg atgtcatagc ctgattgcag
     1081 gaaggccgcc aggttggtga tgtcatacga cggatagcgc ccggtggtgt aaatcttctt
     1141 gttgttcagc cctccggaag cgcttgcctg agccaggtcg aagaacatct ggttggaggt
     1201 aaagcgctca tgatcagcat ccagccccca ggtgatttgc cagccgtcca tcggtttgct
     1261 gttcagagtc agtttcatgc cgtactggtc ggtgtcctgc tgtgacgaag agaaagccgt
     1321 cacctgtttg ttcgcattta ccgtcgggaa cgggtagaat cgcaacgact catcgcggta
     1381 gtaaacctga ccgaccagct cctgtcccag aaaagcgctg tcagagtact gcaggctgat
     1441 caaatgccgc tcagtgccgg gaatacggtc ggaattcagc ccgttactga cgaatggcgt
     1501 gctggtccct ctgatggcag agaagccttt cccgagatta agcccgtaat cgtcgtcgcc
     1561 ctggctttta tagtactgtg tgatcaactg aagctgccgg gattcatcga tgttcagcgt
     1621 accagttccc atgatgtcca gccgatcgga gtactgcagg ccggtctggg tgttatcaag
     1681 caaggtggca tcgccgttac cgtcaaacca gccgccaaat ttctgatatg ccacggaaag
     1741 acgtccggag atatgctcat ttccgccgga gacagctccg gcaatgcgtt catcgtgatc
     1801 tttactgctg ctaaagccac ttttggtgcc agcctcaaac tccatcatgg tttccggctg
     1861 gccttttttg gtcacgatgt tgatcaggcc accggtactg ccgccgccgt acagggacgt
     1921 cgcaccggag atcacttcaa tatggtcgat attaaaagga tctatagagt ccagttgtcg
     1981 gctgtcggta cgtgaagagt tgagacgcac gccgtcaacc agcacgacca gcgggcggcc
     2041 acgcacattc ataccgtagt tggtgcggct ccggctgctg acgtcaaggc cagggatcag
     2101 ctgagccagt gcgtctttaa gctctttgcc gccctgaatc tgctgttcca gttcggcgtt
     2161 ttcgataacc caggtggttt gcgccatctc cgctacggtg cgattgctgc ggttggcaga
     2221 caccacgaac gtttcatcat cggtttgttg agcgactgct ggcgccatca tggtaagaag
     2281 cagtgggttg agagcccaaa gcgtatactt tttgcttatc atcattattc ctccatacgg
     2341 gcggaaaaaa tcagcccaaa gatgctgtgt agcgagttaa agaagcagcc tccggctgcg
     2401 ttttccctag gtgccgctgc gccactgaat cagggcgggc ggcatactga gatcgaataa
     2461 atcacgtccc attacgcgat taagaatacg tgcagatctc catgccatca ggctgagctg
     2521 gggttcggcg atgccatggg tttgcatact ggcgttgacc acgaagatgt tgttctcttt
     2581 cgggccactc cattccagag tgaagtcatc acgcacttta aaggtgttct tatcgtgcat
     2641 ggtgatcagg ggcatcagtg agggaagtat ttgtggcaac gcagaacggt aacctgtggc
     2701 gaaaatcacc acatcacttt ccaggctctc cctgccctga tccagatgat gctccatcag
     2761 caacttccag ccaggaccac tactttccag agttgttacc gagcggctgg gtagcagacg
     2821 gatatttctt ggttttctca gaacttcaaa acggtggtac aactcacgat aaatggtcag
     2881 taaagaatcg gcagtgatgc catccgatgt cattttctgc tcatccagta actgatggcg
     2941 aatatcttcc tccagtccgg agaagcctga aatatattca ggtgtaaaat actcatcagc
     3001 aaaagcagcc tcatccagtg cgttaaaatt attacgccgg gagacccagt ttatttccgc
     3061 cgcttctccc cattccccgc gtaatgcatt aaggaacagg tctgcaccac tctgtcctcc
     3121 accaaccacg gttatccgtt ttccactaag gtccggccga cgaagattca tttcactggc
     3181 atggaaacag gattgtgtca tatgcttcac acagggtggt aaataaggtt gttttcctgt
     3241 accaaggcag atattgcggg caaaatattc tccctggctg gtttgcacca gaaacaatcg
     3301 acgtttttta tcgaaatcaa tgttttcaac ggtatgactg aaatacaggt tattcatatc
     3361 ttcagcagcc cagcggaggt agtcagaaaa ctcttcacgg gatactgtac gtagtctgct
     3421 tgtaaggaag cgatagaact ttttgtgctt caccagatag ttaacaaaac tgtagggatt
     3481 tgtaggtgca acagcactga ccagatcttt cagaaagacg gtctgcatat gacaatccgg
     3541 taccagcata cccggatgcc aggaaaaatg aggatgttca tcaaagaaga gacagtccag
     3601 ttcttcgatc tgatgtgaca acgcagcaat gctgagatta aatggccctg tccctacacc
     3661 aataaaatcg acactttttt tcatgattca tattcctgag ttaccagcca cagcggattt
     3721 tgcagatcct caaggtaatt cggcagcatg cggctgccgc catccagatc aggccaggtc
     3781 agttttaccg ggttcagcac cacgcgaatg atttgtggcc tgaaaagtga gaaaagcgca
     3841 aaacgctctg acatttgtgg atgttttttc atgtaatcac tcaacactgc tgccagcagt
     3901 tgataaaatc gcctttcagg tacgccaaga cgaaccatca gtggcgaaat aaaacgcagt
     3961 actgtcacga agtgacccgt ctgcaaatca tggattaagt agtccgcact caggcgggat
     4021 gtaacatcac gaacctcctg aggcaaagag tccatttcgg ggaactcttc tttcaccagt
     4081 cgcatatcgc cctggaagtc tttcagcaga acacgctgtg gaaccccctc tttcatggcg
     4141 agagttatat tttgtccatg tgcaataagc gcgacaccgt agcggcaaag caggtgatac
     4201 agaggaacca cgaccacccg gaacagttgc gtaagccagg tttcagcgtc cagcccggag
     4261 cggtctatat atgcgcctgc cagcggctga ttgttttcgt cgcactccat cagtgttgcc
     4321 atcagaaccg gactttcatc cggtttcagc cagcggcacg gattctcccg ccagataaca
     4381 ccaagcattt cctggtagcg atagggagcc cgggcaagcg cggcatagcc ttcatgggag
     4441 acatagcctg cagccggttc accaagtatc actgcgccgc tttgcactag ggtggcgtcg
     4501 gtcgcaaaaa cctgttgtag ccagcgtgaa gccagtggtc cggcagcgat gtatctgcca
     4561 gggatccccc ggtagcatga ggtgttgtag atggtcagcg gcagcttgat atccagcccg
     4621 ccccggcgac tggcgttggt cagggtacgc agcgactgct gggcgagcca ctggtcgcca
     4681 aactcgccga gagacaccat cctgccttcg gcaaaatcag cgatgaagtc ggtagcgatt
     4741 ttttgctgcc actgccacgg atgtaccggc agcggcagcc agttatgatc cagtccgttt
     4801 tcctgccaga cctgactgaa gcgggcaaac tcctgcggat ccattgcggc cgtcaacaac
     4861 tgatgaatat ccatctcgtt atcacagcgc cagatcatat gttcacgttt taccgccagc
     4921 cagtgcagtc tgaaggtgtt ggcatactct ggcgcatatc gttccagcgc ctctttaccc
     4981 cagccacggc gacctttatt aaaaacgaat ttaggatgac cgctcagcag gcattgcagg
     5041 cggtcggcat taagattaat caggtcactg gcgctcagcc cgcgacgggc tttcagtagt
     5101 tgcaggtcgc ccagcagcgt ggcatacaaa tcctgcatat gctcagcaac ggttgcatcg
     5161 ctcattgaca gtacctgctt tagctgcatc agcagcgtct gagccagtac tggctcgtcc
     5221 gcgcagcgca gagtttgagc atcaatccag agccagcccc agataccacg ttcagcgatg
     5281 aagcgccatt gtgctcccgg caggttaatg cagtagcggt catcgccctg agattccgcg
     5341 tggaaaacct gctcatactc cagctcagac aacatttttg ccaccaggcg gcggttgacc
     5401 aaatcccagt ctttgtgatt cattacagac cgacctccgt gaagaagtga tgacgatccg
     5461 ccatcaccat gcgcgagcgt ttatgtggga agtcgaactc tttaattgtc cggtatcccg
     5521 caggctcaag atggcggaac aggcgctggt tatcggtgcg tggctccagt actgtgcgct
     5581 gcgtacgtgg ctcattcagc agcaggtaat gtgtcacccc gcgcagccag ctttgcacat
     5641 agtgggctcc gcgccattgc tgttcaccaa ccagcagatg caggccacgg tcaaagggct
     5701 gccacgaata gtggcgacca atgcggtctt ccgccgccca gtagatttcg aaataactga
     5761 acggccgatc gtcgaaacaa ccaatcagcg ggaatgcatg tttactggtg agctggcgtt
     5821 ccagataggc gatctgtacc tccagtgagc cactttgctc ccagaaatac tcaacgcgcg
     5881 gatcgttcat ccagcgagtg aaacgttctg catcagaaac gggatcggca atgcggaaac
     5941 tcagcatcct gcgaatgcgt ggatcgtaac gacgataaac ttcaccctgc ggacgggggg
     6001 ggcgctgcgg gaaataaatc tcacgctctg catcaaaaac catttcaccg gaggcccggt
     6061 ttgccggaga actcagccat aacggtaact gccagaaagc ggaacgatgg ataatgtcgc
     6121 tttgtacctg tccgaaaagc gtcagcgcct gtggctcctc acaccattcg gaccagggaa
     6181 gaaccactgc tgaaagttgt ggtgcggcga taaatatctg atccagcgcg tcgcacagcc
     6241 accctgttgg cagctccccg ggccagtgca acaccgcgct attgtccagc ccccagccaa
     6301 gattcagagg cttgtcgagt ttttcacagc gcagtccata tccgctgtga acaatgtttg
     6361 ccccagacat tcagacctcc tgagcctgca gcgggttagc gaaatcaaaa tagatcaccg
     6421 aagggtcaac gatggtgttc tcgttatgat cgttcagata acagaaaaag ttacctttta
     6481 cgttccagta cggactttcc agaacatagt tgagacaggt tttatgagtc acctggtcac
     6541 gcacttcagc cagcaatgtt cgtacacgag ccatcagatt cgcttcgctg tccagcccgg
     6601 cagcacccag cgcagcagtt acggcaaaag tggagttaac cagcaggtaa taagggaaat
     6661 agcgcagcaa ctgctcacgg gtgaaaatat tttccgcctg cgcctcgtca atggtatcga
     6721 gccactctgt cgcgtgaggc ataaacgcgc tgccctggca gtcacggtaa ataaatccga
     6781 ccggcagatc cccaagcatc tgcacaagaa tattttgctg atgcgccagc agcaccaggc
     6841 cgtaatccgc ttcagccgta aacagaggct tcagcacctg ctgacagtaa gcatcgaccc
     6901 atgcgtgggc ggcctgttgc acagtgatac cgaggcgatc gctcaggcgt tttaccgccg
     6961 aaaccagcag cgaatcaccg ccatccggtg cggcctgagt cagggaaacc agaacgttgg
     7021 tctggctttg cggctgctcc agcagcaggt tttcacgcag ggaaaacaaa ctttcctgca
     7081 tgatgttgcc gttaagatcg cgcagcccgg cccagccgtc ctcctgcatt acccggaaag
     7141 tagggaagcg ggcctgtagc atctgccagc cgtcggtttg cgccagacgt gccaggcgca
     7201 ttcctcgctc cacctctttc acagacagag tacggacgga gttggtcagc cgaacgctca
     7261 gggagaactt gatcatatcg cggctggtag cacagtagag ggagcgggaa gaggtggtcg
     7321 gcagccacga cgtgccggcc tcgcctaagt ctctgataag tcctttagca aaaagtgcct
     7381 ggcaccacac ttgctgcaaa agatattctc cctgccacgg gtgcagcggg aacagccatt
     7441 gattgtcact taattcgttg agtaactggg gcgcattttc tgcggcaaat cgcgtcaacc
     7501 gctgttgaag gttaagatgc agactttcac cagcgatttg cgttttatcc accgaaaacc
     7561 accgcagcgg gaagtgaggt gccatgtcag gcaggtatcg ttcagcttcc tggcggttaa
     7621 acggttcatg agacttaggt gcagggtgga aagcgtgtcc tgtcagcagt gcctgctcag
     7681 cctcgccaaa attcaacgct ttttcacgca ggatggccca gtcatggcgg gcatcaattg
     7741 tctgttgcgt atgggcgtag ctttccagca cgcgttggtg gaaagtttcg cagaggggaa
     7801 ctggcagaaa aagttgatgc cggagcttat caataataag ccgggagaga gtggtaaagt
     7861 cgacaggata actgcctgat gccgtgacca gatgagcagg aaatgcaaag cggtgatgct
     7921 gggttgggga gaaataagcc actctgaaat ggagcgactt ctgttcgtcc agcgggataa
     7981 tcaattcgtc tggcgggtat tccgccagtt gccagtcttt ggtttcacga atcaaggcat
     8041 tcaggaagca ctgcgcagcc acatctgtgg cggatttttc agagggcagg atcataatca
     8101 aacagctcct acacagtaaa ataataacaa ttatgtcaat aatgattctc attatcaacg
     8161 agaaatgtta ttgtcagaaa aattaatttt acaaataaac acaattaata catggtgttg
     8221 aaacattaca atgtcatcta agatcgaaga tacgccccaa aaaaccctgt cctgctggcc
     8281 actggcgttc agtgccggtc ttctcggtat cggacagaac ggtctgctgg ttgtactccc
     8341 tgttctggtc atacagacaa atctgagtct gtctgtatgg gctgccctgc tgatgctggg
     8401 ctcaatgctg tttctgccat cttccccatg gtggggaaag caaatttccc ttactggcag
     8461 taagactgtg gtgctgtggg ctctgggagg atatggcgta agctttaccc tgcttgggct
     8521 gggaagcgtg ctgatggcta ccggtgccgt aacaacagcg gtggggttgg gaatattaat
     8581 catcgcccgg atcgtctacg gtctgaccgt gtcagcaatg gtgccagcct gtcaggtctg
     8641 ggcattgcag agagcgggag aagggaatcg catggccgct ctggcaacca tcagctccgg
     8701 cctgagctgc ggcaggctat tcgggccgct gtgcgcggcg gcaatgttgg tcattcaccc
     8761 tctggcgcca gtgtggatgc tgatggcagc tccggtgtgg gctgtggtga tgcttctgcg
     8821 gttgcccggc acaccaccac agcccacacc ggagcgcaag agcgtcagcc tgaagcggga
     8881 ttgcctgcct tatctgcttt gcgcaatgtt actggctgcg gcaatgagca tgatgcagct
     8941 tggactttcg ccagccctta ctcgccagtt cgatactgat accaccacta ttagccaaca
     9001 ggtggcgtgg ttgttggggc tgtccgcaat agctgcgctt atcgcgcagt tcgtggtact
     9061 ccgtccacag cgcctgactc cagtggctct gctcctgagt gccggggtgt tgatgagtag
     9121 tggtctggct atcatgctca ctgaacagct atggttgttt tacctgggct gtgcagtgct
     9181 gtcatttggg gccgctctgg caacacccgc ttatcaactt ttactgaatg ataagctggc
     9241 cgacggcgca ggcgcgggct ggcttgcggc cagtcacaca cttggctatg ggctgtgcgc
     9301 attgttggtg ccactggcgt cgaaaacagg tgtctcaatc gcgctgattg tgacggcatt
     9361 atttgccgct gtattattta ccatagtgtc tgcatgtatt tggcattacc gtacaatcaa
     9421 gtaaggctat gatgttgtac tcacagccgg cataacgtcc tgtgcaattt cgctgtcgaa
     9481 accgcctgtg ctgaccgccc agtgcactca cctgaagcac atcgcagcat gccaccagga
     9541 tgccagatcg tcttctgaaa tgggtggata cattggcaac gttaaccgca acaatttttc
     9601 agagagaatt ttggggggtg agggccagtg atgtaaaaaa tacgccaaga ggttaattca
     9661 ttgtttatac tgatatgtca ggtaatgcgt ttgcagtcat tacagtcgtc atggagactg
     9721 taatgactgc aaacgcatac catggagcga aattctatcc taagcgtcac attagttgat
     9781 aaacctgtaa cgttaatgaa ttctcatgcg aaaagtatgg ttatgataaa aaagcctaaa
     9841 gctgtcatga tgagggagcc aatgacgtga acaagtacag acgtcaaagc ccatatataa
     9901 tttcccgcct ggagtaatgc gaatacttca acagaaaatg tagagaaagt actcagacct
     9961 ccacaaagtc ccgttgttat cattaatttc caaaaaggat caaggtgtgg ttgtcgtaaa
    10021 aagtacgcca acgcagtacc aataattaac ccggcaagga ggttcaccac cagtgtacct
    10081 ggaggtagat ttggaaaaag actgttaaag cgtgtggaca gtaaccagcg cagagtacat
    10141 ccaacagaac cgccgataat gacggcaaaa agtgatttaa tcatgtaccc ctcataaaat
    10201 aattttttat cgtaacaata tcagaatatg ttctcatttg actgatattg ttaatttata
    10261 tcctttgact acatgcaaga taagggaagg ttctatttat tgacattaat accctcgaca
    10321 attgctgagc agaagccccc acgacaagtg acagaatgac aacatctaca gtggggagcg
    10381 ttttatcgat gaacaagcaa ccaagaagta acacggcata ggtgacgaca atgccagtaa
    10441 gcatagaaat aacaaaaacg tgagctttca tcattcctcc agaataaccc cgacgtgtaa
    10501 gcctggggga ttggcacaat aatgtcattt aaaggcagac tttccggcaa attgcgccga
    10561 tgaacccagt tcatcctcaa ttctcataag ctggttatat ttttcaatac gctcaccccg
    10621 gcatggagct cccgttttga gatgacctgc gcgcattgca actgtcatat cggcgataaa
    10681 gctgtccact gtctcaccac tgcggtgtga gataaaggtg ccccagttgt tgtcatggca
    10741 aagttgtacc gcatcaaagg tttcactcag ggaaccaatc tggttgagtt taatcaatgc
    10801 tgaattggca aggttttcgt caatgccgcg ctgaatatac tttacgttgg tcacaaacag
    10861 atcgtcgcca accaattcta tctgctctcc caatgcacca tgcaggattt tccatccagc
    10921 ccagtcatct tcagctaccg ttaagggcag gaagatcaac taggtgctct ggggctggtg
    10981 accaatgcgg ttgtgctgtg aaacacactt tacatgcagg aggcgttgtc acatcttcgc
    11041 aaatcgggag aaactcctga ggagtagcat ccggcaagat tatcgctgct gatacatagc
    11101 catataaata tgctggggca ttatacgtct tcgttaccgg aagatattct taagggtgag
    11161 ttaagatcat taaacttcaa tacaaacaat taattaaact cttagcgtgg ttttttacat
    11221 cactggccct cgccccccaa ttttctctat gttttgccac tacttccctc attttactgc
    11281 gctgtttgct acccctgaga atcgacatta cttgctatcc agtccgcagc aactctctgg
    11341 caacaaaact tatcagaatc ataagtctgt gcattattcg ttcaagcgca tcgccaataa
    11401 gcggatgttg ctggccgagt gttctgctaa tcggatgata acaaatcccg tggttcggca
    11461 ttttcctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa catgaccgcg
    11521 aataagacaa caattgtcgg agctggcgcg ctgtcgataa aaaaggacag ccagacacca
    11581 gtcattgaga ccaatatcga tatgccagtc gccagcagga gcgcaatatg aaagcgttgt
    11641 gtcaggagta ccgcaatggc accaggcgca atgagaagag aaatagagag aatgatacca
    11701 accgctttca gcgtcgccac aatggtcagg gagaccatgc acagcagccc ataatgcagc
    11761 catctcgtat gcaaaccgct tgcctgcgcc tgttgataat caaagctgaa cagcaaaaaa
    11821 tcccgccatt ttacgctaat aacaagtgta accagtccgg caataatcac cgtctggatt
    11881 atatcgccga tggttatccc gagcatgtcg ccgaaaagaa tatgatcaag atgtacgtct
    11941 ggtttgactg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
    12001 atcactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcacag
    12061 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggcaagcacg
    12121 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaact tttcaggacc
    12181 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
    12241 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
    12301 ctttatgcgc acgccgacga tttgccaaaa gcccatgggt gggcgcaaag acaaatgcta
    12361 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
    12421 aactgaccca cacgccgagg aaactggtca cactgccaat agttacagca atcatgagca
    12481 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
    12541 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
    12601 agaagaggat ttttaatctg cccggatgca atccaatagc acgggcgtga ttctcgtcga
    12661 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
    12721 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
    12781 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
    12841 acgagaagat caggccgatg atggcatctt cttttaagcg agtgagttgg ttaagaaaca
    12901 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
    12961 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
    13021 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accatcgccg
    13081 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
    13141 ttcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
    13201 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
    13261 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
    13321 catgaccgta taatcgcaaa atgtcgtgac ggatccgaga ttgtgcgttg agacaagcat
    13381 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
    13441 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caatcgctct
    13501 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgtc gatggcgaaa
    13561 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
    13621 gatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
    13681 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
    13741 cgcctgtcgt gtgggaatac ccagaacaga tatcttcccg ctggtcagac gcacaaatcc
    13801 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
    13861 agatccgcct ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
    13921 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc cctttttccg
    13981 gctttaattc cctgtaccag cgtactggta gtaaccttca gaaggtcgat atacgtaggt
    14041 accgggccgt tttctgtgct cagggaatcg acatagagca caccaccgta gtgcgcgccg
    14101 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
    14161 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
    14221 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
    14281 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgcaatctgc
    14341 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
    14401 taggtttgtg catttatcgg atcgtatttt ataaacgcat cacgaatatt atcgacgtaa
    14461 atcagagcat tatctggcga catccaggca tgggggttag gtttgccctc atagggccct
    14521 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
    14581 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
    14641 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
    14701 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatctgcg
    14761 atgatggtaa aagttgtaat gaccttgaat ttttcagctg cactcgcctg aaatgctatc
    14821 gagcaggtga ggactaggca ccccaagagc atggttactt tttttattga gagcatgatt
    14881 tagtacctat agtgagcaaa attagaataa acatagcaac tactatgttt tatagcagaa
    14941 gctataaaat gtaaataatt atcattagcg attataagat cccaacacag ggattaggtt
    15001 atttcctgct gacgatgtgg tattgaggat aaaagttggg cagcgctttg gctttaaaat
    15061 gaacttgttc aggacgattg gtaatggcaa agcaaaaata atgtaggtta ttggcaagtt
    15121 ctctaggacg caggagagca tgaaaaaccc aggggcagtc actaaatgaa gg
//
LOCUS       DAFAVF010000104        14378 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFAVF010000104 DAFAVF010000000
VERSION     DAFAVF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.104"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            49..405
                     /locus_tag="LGK42_003099"
     CDS             49..405
                     /locus_tag="LGK42_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2659323.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(456..1352)
                     /gene="yhaJ"
                     /locus_tag="LGK42_003100"
     CDS             complement(456..1352)
                     /gene="yhaJ"
                     /locus_tag="LGK42_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBK2659324.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1457..2158
                     /locus_tag="LGK42_003101"
     CDS             1457..2158
                     /locus_tag="LGK42_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBK2659325.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2181..2345
                     /locus_tag="LGK42_003102"
     CDS             2181..2345
                     /locus_tag="LGK42_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659326.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2557..3867)
                     /locus_tag="LGK42_003103"
     CDS             complement(2557..3867)
                     /locus_tag="LGK42_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBK2659327.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3895..5226)
                     /locus_tag="LGK42_003104"
     CDS             complement(3895..5226)
                     /locus_tag="LGK42_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBK2659328.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5501..6865)
                     /gene="tdcG"
                     /locus_tag="LGK42_003105"
     CDS             complement(5501..6865)
                     /gene="tdcG"
                     /locus_tag="LGK42_003105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBK2659329.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6937..7326)
                     /locus_tag="LGK42_003106"
     CDS             complement(6937..7326)
                     /locus_tag="LGK42_003106"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBK2659330.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7340..9634)
                     /gene="tdcE"
                     /locus_tag="LGK42_003107"
     CDS             complement(7340..9634)
                     /gene="tdcE"
                     /locus_tag="LGK42_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBK2659331.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9668..10876)
                     /gene="tdcD"
                     /locus_tag="LGK42_003108"
     CDS             complement(9668..10876)
                     /gene="tdcD"
                     /locus_tag="LGK42_003108"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBK2659332.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10902..12233)
                     /gene="tdcC"
                     /locus_tag="LGK42_003109"
     CDS             complement(10902..12233)
                     /gene="tdcC"
                     /locus_tag="LGK42_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBK2659333.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12255..13244)
                     /gene="tdcB"
                     /locus_tag="LGK42_003110"
     CDS             complement(12255..13244)
                     /gene="tdcB"
                     /locus_tag="LGK42_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBK2659334.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13343..14281)
                     /gene="tdcA"
                     /locus_tag="LGK42_003111"
     CDS             complement(13343..14281)
                     /gene="tdcA"
                     /locus_tag="LGK42_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBK2659335.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3717 a         3862 c         3363 g         3436 t
ORIGIN      
        1 caatacccgg aatagacttt cgtcactttt aattaaaggg atgtttttat gcagtggtat
       61 ttagccgtat taaaaaatta tgttggtttc tctggtcgcg cacgtcgtaa agagtactgg
      121 atgtttactc tgattaacgc catcgtcggc gctattatca atgtcattca attgatttta
      181 ggtttagagt ttccattcct ttctctcatt tacctggcag caacaatcat tccggtcatt
      241 gctctgtgtg ttcgtcgtct gcacgatact gaccgttcag gtgcgtgggc gcttctctat
      301 ctggtaccga tcattggctg gttagttctg tttgtatttg cctgtttaga aggcaattct
      361 ggcagcaatc gttacggaaa cgatcctaag tttggttcaa attaatctta gaattggggc
      421 gacatttcgc ccctttttat taataataac aaatattatt ttccgctaaa aagtttggga
      481 atttcccgca gacaccagga tttcgcttcc cccatactgt cacgacgcca ggccataata
      541 atatcgatct cgctggtcga ttccgggctg acgacacgca accgcccttc cgcaatatct
      601 ttttcgacca tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt
      661 ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg tacggtcaac
      721 accgggcgct cacgagcggt atccgctacc gcaattccac gatatttcac gcgcgtcact
      781 tcagataacg gttccggctc ctgatgaatc gggtgatcag gcgcggcaac gtagacgttc
      841 attaacgtat agagcttgcg cgagttgatc tccgacgagg aacgaaaatg catatccggc
      901 gcgataacaa tatccgcccg cccctgctcc agtcgttccc acgcccccgc cagcacttcg
      961 gtgatgattg ccagttgggt attggctttt gccgccagtt tgtcgattaa cgggaaaaag
     1021 gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg
     1081 agggcttccg catcggtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc
     1141 agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc
     1201 acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc
     1261 agctcatccg ccgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt
     1321 gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat
     1381 accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa
     1441 agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta
     1501 cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt
     1561 aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca
     1621 gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta
     1681 tcgcgacagc gaaggcaatc atgttcaggc cagcgccggt gaggcgttgc tgctctctac
     1741 ccagccgggt gtcagctaca gcgaacacaa tctcagcaaa gacaaaccgt taacacgaat
     1801 gcagctttgg ctggacgcct gcccgcagcg agagaatccg ctgattcaaa aactggcgct
     1861 taatatgggc aagcagcaat taatcgcctc gccagagggt acgatgggaa gcctgcaatt
     1921 acgccagcaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca
     1981 gttgcatggg ccacgcgcgt atttgcaatc gattcacggg aaatttcatg cgcttacgca
     2041 tcatgaagag aaagcggcgc tgacctgcgg tgatggggcg tttattcgtg acgaggctaa
     2101 cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt
     2161 gtttttacag ggagatgatg atgagtaaaa aatcggccaa aaagcgccag ccagtgaagc
     2221 ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga
     2281 gcgagctgga agccatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg
     2341 cgtaaagaca ggttgccgga tgcggcgaaa acgccttatc cggcctacaa aatccaacaa
     2401 cattaacgaa ttaaacaacc cgtaggccgg ataagatgcg ccagcatcgc atccggcatc
     2461 aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg taggccggat aaggtgcgcc
     2521 agcatcgcat ccggcacgat acccaaaacc tggcgtttat ctggccttgc tcgccataat
     2581 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2641 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2701 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     2761 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2821 catcgagatg gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2881 tgctgcggtc gttgcggcac acagcgcaga caaacgcggt aactggttat ggatgtaaat
     2941 tgcgctcaaa tgagaaagca tcagcgcacg cgccagccgt tcatcatccg ctccgaagtg
     3001 ttctgctacc accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt
     3061 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     3121 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     3181 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     3241 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     3301 gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc
     3361 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     3421 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     3481 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3541 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3601 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3661 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3721 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     3781 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     3841 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     3901 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     3961 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     4021 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     4081 tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     4141 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     4201 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     4261 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     4321 cgccagcgcg gaaatattct gctcataggc ttttaccgct tcgtcatgtc ccatcgccag
     4381 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     4441 tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgagc gataagagat
     4501 caccatcggg cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat
     4561 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     4621 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     4681 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     4741 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     4801 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     4861 cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     4921 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     4981 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     5041 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     5101 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     5161 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     5221 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     5281 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     5341 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     5401 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     5461 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     5521 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     5581 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     5641 tccgcgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     5701 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     5761 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     5821 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
     5881 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
     5941 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     6001 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     6061 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag
     6121 aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca
     6181 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     6241 accctgcgtc aatttccgct ttactgcgca gcgctagctc gttgtgcatc atcaggccag
     6301 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     6361 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     6421 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
     6481 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     6541 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
     6601 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     6661 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca
     6721 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     6781 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
     6841 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     6901 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
     6961 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
     7021 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     7081 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
     7141 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
     7201 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     7261 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     7321 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
     7381 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     7441 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     7501 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     7561 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
     7621 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
     7681 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
     7741 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
     7801 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
     7861 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     7921 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
     7981 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
     8041 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt
     8101 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
     8161 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
     8221 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
     8281 taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
     8341 taccaatcac cattgggctg acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag
     8401 tacgcatcag atcgtcattt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
     8461 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
     8521 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
     8581 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
     8641 acagcgaatc aaattccggt gtacgcagga agcgtaccat acggatcttc atgatgaagt
     8701 gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc agctttaaag tcgcgctcaa
     8761 tgtagatatc gaggaacgat gccgtgcggc ccagcgacat cgcgccgcca ttttgcgatt
     8821 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg
     8881 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
     8941 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcctcg cctttttcca
     9001 gacgagactg gagatcggca aattgcagtt cgcgttcacg taccagataa ctgatgccat
     9061 acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac
     9121 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     9181 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttctc
     9241 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacggg tgtagcgcac
     9301 gtttcaacgg cgcatccgtt tgcaggccaa caattttttc cagcggctgg ttaatatatc
     9361 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
     9421 ttgcattttc gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg
     9481 tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
     9541 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     9601 acagcttatc gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc
     9661 aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag
     9721 caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg
     9781 aaacaattcg ctcaccacag gagttagagc gattattcat ttctgtatca atctctacgc
     9841 ctaatacagc caaatgttcc atgaccagac gacgaattaa gcttgaattc tctcctattc
     9901 cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac
     9961 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
    10021 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
    10081 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
    10141 acatcgcacc aaagtcgaca tcgccactgc gggtacccat catcaagcct tccagcggcg
    10201 tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
    10261 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agcgaatgtg
    10321 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
    10381 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
    10441 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
    10501 taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    10561 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    10621 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
    10681 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    10741 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
    10801 cgctggcatc gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    10861 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    10921 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    10981 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat
    11041 gcgataatcg gtgcgcccat ggcttcaata aggtcgagga tgttcgggtt ggcgtaggca
    11101 acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac
    11161 actttagttt tgtcgccttt ataaccgaac ttcagaatca gaccattcaa gccttccagc
    11221 gtccccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    11281 gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    11341 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    11401 agcgtaaaca ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    11461 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    11521 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc
    11581 atgatggaaa tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc
    11641 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    11701 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    11761 tccttaccaa accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg
    11821 cgattcagcg gtgcaaatcc gagctggttt tcccagaacg tcataaaggt attggtaata
    11881 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    11941 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    12001 gagccagaaa gacacagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    12061 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    12121 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    12181 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    12241 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    12301 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    12361 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    12421 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    12481 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    12541 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    12601 ccgtgaacgt tttctgactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    12661 gccaccgcaa taccagcaat taaaccacca ccaccaattg gcacaatcac gttatcgaca
    12721 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    12781 tcatcgtaag gtgggataaa aatacgacct tccatttcga caatttcgct cactttagcg
    12841 atagtgtcgt tgaaattatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    12901 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    12961 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac tacaccttta
    13021 cgtttttccg catcagtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    13081 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    13141 tagtttgagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    13201 ataatgtcat caatagcaac cggcagatcg tatgtaatat gcattataaa acctcttcgc
    13261 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt
    13321 atgtttaaat aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat
    13381 tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat
    13441 aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag
    13501 taataaattg atttgaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat
    13561 cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa
    13621 tactgatgcc atttctttgt aacgtagtaa gaagctcgct gtagtacccc atattggttt
    13681 gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc
    13741 gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct
    13801 gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg
    13861 ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct
    13921 ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa
    13981 atgagacttc caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca
    14041 tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta
    14101 caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga
    14161 ttttactgac ggctggttga gttaacccta attcttttgc agccgagccg atagagccac
    14221 ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca
    14281 taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag
    14341 gatatgtgcg accactcaca aattaacttt caatactt
//
LOCUS       DAFAVF010000105        14321 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFAVF010000105 DAFAVF010000000
VERSION     DAFAVF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.105"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            131..1369
                     /locus_tag="LGK42_003112"
     CDS             131..1369
                     /locus_tag="LGK42_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK2659336.1"
                     /translation="MTNYRVKSSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(1510..1836)
                     /locus_tag="LGK42_003113"
     CDS             complement(1510..1836)
                     /locus_tag="LGK42_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBK2659337.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(1951..3207)
                     /locus_tag="LGK42_003114"
     CDS             complement(1951..3207)
                     /locus_tag="LGK42_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBK2659338.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            3411..4376
                     /gene="glk"
                     /locus_tag="LGK42_003115"
     CDS             3411..4376
                     /gene="glk"
                     /locus_tag="LGK42_003115"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBK2659339.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            4595..4921
                     /locus_tag="LGK42_003116"
     CDS             4595..4921
                     /locus_tag="LGK42_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBK2659340.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            4943..6190
                     /locus_tag="LGK42_003117"
     CDS             4943..6190
                     /locus_tag="LGK42_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBK2659341.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            6205..7290
                     /gene="ypdF"
                     /locus_tag="LGK42_003118"
     CDS             6205..7290
                     /gene="ypdF"
                     /locus_tag="LGK42_003118"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2659342.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            7290..8327
                     /gene="ypdE"
                     /locus_tag="LGK42_003119"
     CDS             7290..8327
                     /gene="ypdE"
                     /locus_tag="LGK42_003119"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK2659343.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            8352..10847
                     /gene="ptsP"
                     /locus_tag="LGK42_003120"
     CDS             8352..10847
                     /gene="ptsP"
                     /locus_tag="LGK42_003120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK2659344.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(10850..11707)
                     /locus_tag="LGK42_003121"
     CDS             complement(10850..11707)
                     /locus_tag="LGK42_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2659345.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(11720..12454)
                     /gene="ypdB"
                     /locus_tag="LGK42_003122"
     CDS             complement(11720..12454)
                     /gene="ypdB"
                     /locus_tag="LGK42_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBK2659346.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(12469..14166)
                     /gene="ypdA"
                     /locus_tag="LGK42_003123"
     CDS             complement(12469..14166)
                     /gene="ypdA"
                     /locus_tag="LGK42_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBK2659347.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3334 a         3642 c         3920 g         3425 t
ORIGIN      
        1 catcaatctt ttcgtagtca taatcctggt ctatcaaaga aatcaccaca acccattcaa
       61 atgaatttga taatcattct cgtttggcat agcatgaaac atagcaaagg ctatgtttta
      121 gaggcacaag atgacgaact atcgcgttaa gagtagcagc ggacgggcgg cgcgcaagat
      181 gaggctcgca ttaatgggac ctgcgttcat tgcggcgatt ggttatatcg atcccggtaa
      241 ctttgcgacc aatattcagg cgggtgctag cttcggctat cagctactgt gggttgtcgt
      301 ttgggccaac ctgatggcga tgctgattca gatcctctct gccaaactag ggattgccac
      361 cggtaaaaat ctggcggagc agattcgtga tcactatccg cgtcccgtag tgtggttcta
      421 ttgggttcag gcagaaatta ttgcgatggc aaccgacctg gcggaattta ttggtgcggc
      481 gatcggtttt aaactcattc ttggtgtttc gttgttgcag ggcgcggtgc tgacggggat
      541 cgcgactttc ctgattttaa tgctgcaacg tcgcgggcaa aaaccgctgg agaaagtgat
      601 tggtgggtta ctgttgtttg ttgccgcggc ttacattgtc gagttgattt tttcccagcc
      661 taacctggcg cagctgggta aaggaatggt gatcccgagt ttacctactt cggaagcggt
      721 cttcctggca gcaggcgtgt taggggcgac gattatgccg catgtgattt atttgcactc
      781 ttcgctcact cagcatttac atggcggttc gcgtcaacaa cgttattccg ccaccaaatg
      841 ggatgtggct atcgccatga cgattgccgg ttttgtcaat ctggcgatga tggctacagc
      901 tgcggcggcg ttccactttt ctggtcatac tggtgttgcc gatcttgatg aggcttatct
      961 gacgctgcaa ccgctgttaa gccatgctgc ggcaacggtc tttgggttaa gtctggttgc
     1021 tgccggactg tcctcaacgg tggtggggac actggcgggg caggtggtga tgcagggatt
     1081 cattcgcttc catatcccgc tgtgggtgcg tcgtacagtc accatgttgc cgtcatttat
     1141 tgtcattctg atgggattag atccgacacg gattctggtt atgagtcagg tgctgttaag
     1201 ttttggtatc gccctggcgc tggttccact gctgattttc accagtgaca gcaagttgat
     1261 gggcgatctg gtgaacagca aacgcgtaaa acagacaggc tgggtgattg tagtgctggt
     1321 cgtggcgctg aatatctggt tgttggtggg gacggcgctg ggattgtagt tgaatgagcg
     1381 tcgcatctgg cactattggc ggatgcggcg taaacgcctt atccgcccta cgcgttaaag
     1441 agccggtttg taggcctgat aagacgcgct agcgtcgcat caggcattgt gctccattgg
     1501 aggacgcgtt taatggcggt gcccatgtcc gcggcctttt ccgcgatgat cgtcacgatc
     1561 gcgccagcct tcacgatagc cacgctcata tgctttacgc ttatcccagc cacgatgata
     1621 gccattatca tgacgccacc agcggttttt gcgccattca taattacgat gccagtaatc
     1681 acgatctcgc cagtgaccac cgtcccagta attaccgtaa tgatcgcgat cgccaatttg
     1741 taatttgatc gatggcagta gggtgatttc acctgcattt gctgcaagcg gggtaaatgc
     1801 catcagggcg gccgccagaa acagtgacct gaacattgtt attctccttc acgatcgaag
     1861 ccgtcagcgg cctgttaacg caatattacg gggaggtaaa gccccgtatc atcgccataa
     1921 ctcttaaatc ataaaggaga gcattttttg ctaaacatta tcgtggggtg gctgttgctt
     1981 cggacgattg accatcatca tcggcttacc tgctaataac agccatgccg gaagcatgac
     2041 gatacagagc agcggtagca atgtggtatt gggtacaacg actgccgcca taaaaagact
     2101 taaccagcca tcgcgtgtta ccaccagcac gatgccgaga atagcgcagg aaacggttat
     2161 tgccgctgga acggcgggaa cgtgctcatg cagcatcaac cctaatgcca cgccgacaaa
     2221 cactgccggg aagattcgcc caccgcgaaa gccactggcg gcagcaacga ccagggcggc
     2281 aagtttaatt accgccagca aaaagtaatc gctggtgctg aaagcctgat ttgccaccat
     2341 ctgctgcatc tcatccagcc ctttaaacag cgaaaccggc ccaccaataa ctcccagaat
     2401 accgagaata aatccgccaa tacctagcac gagcaccgga tttttcattt gatgcatcat
     2461 cgcgtgcaac cgtggtaagc accatacggc gaccattcct gctgcgatgg caatcgccgc
     2521 gacaattgca ccgctgagaa tatcggtcat ttccatctgt ccgtaatgcg caatgggcag
     2581 tgaaaaatgg ggatggaaaa ataatccggt agtaagtgca ccagctgctg ccgccattaa
     2641 tggcgcaaaa agacgatccc atagcggaac ttcactactg ccatttaagg tttgcgaaaa
     2701 tatcaacgcc gccgcaacag gtgtaccaaa cagcgcgccg atggttccgg cagaggctaa
     2761 aatagtccac tccattcggt tgacgcgcgg taacagacga gcgccaatgg caaccgcaag
     2821 ggcgatattg acggtcatga tcggatgttc cggccccagg ctgacgccgc cagcaagacc
     2881 gagaattaat gctacgataa gtcctggtag cgcagagggc ggaactggtg cgccgatcag
     2941 cggttcacag gcggggtctg gtccggcatg accctggctg aaacggataa ccaaccccac
     3001 cgcaataccc gttagcgtta atacaccgat gatccagaga ggtgaatcct gggctatccc
     3061 cagagttccc ggcagtcgtt gccagagcaa attctgtaat accgaggcga ttttcatcac
     3121 cacaatcaga ataagactgg acgcaatccc aattgccacg gcggggagcg ataataacaa
     3181 catggttctg gctcgcggat ggagcatgtt ctattcctta tgcggggtca gatacttagt
     3241 ttgcccagct tgcaaaaagg catcgctgca attggtgctg aaacgataaa gtaaatgtgt
     3301 gacccagatc gatatttaca gggagcctgc ctttccggcg ttgttgttat gcccccaggt
     3361 atttacagtg tgagaaagaa ttattttgac tttagcggag cagttgaaga atgacaaagt
     3421 atgcattagt cggtgatgtg ggcggcacca acgcacgtct tgctctgtgt gatattgcca
     3481 gtggtgaaat ctcgcaggct aagacctatt cagggcttga ttaccccagc ctcgaagcgg
     3541 tcattcgcgt ttatcttgaa gaacataagg tcgaggtgaa agacggctgt attgccatcg
     3601 cttgcccaat taccggtgac tgggtggcaa tgaccaacca tacctgggcg ttctcaattg
     3661 ccgaaatgaa aaagaatctc ggttttagcc atctggaaat tattaacgat tttaccgctg
     3721 tatcgatggc gatcccgatg ctgaaaaaag agcatctgat tcagtttggt ggcgcagaac
     3781 cggtagaagg taagcctatt gcggtttacg gtgccggaac agggcttggg gttgcgcatc
     3841 tggtccatgt cgataagcgt tgggtaagct tgccgggcga aggcggtcac gttgattttg
     3901 cgccgaatag tgaagaagag gccattatcc tcgaaatact gcgtgcggaa attggccatg
     3961 tttcggcgga acgcgtgctt tctggccctg ggctggtgaa tttgtatcgc gcaattgtga
     4021 aagctgacaa ccgcctgcca gaaaatctca agccaaaaga tattaccgaa cgtgcgctgg
     4081 ctgacagctg caccgattgc cgccgcgcgt tgtcgctgtt ttgcgtcatt atgggccgtt
     4141 ttggcggcaa tctggcgctc aatctcggga catttggcgg cgtgtttatt gcgggcggta
     4201 tcgtgccgcg cttccttgag ttcttcaaag cctctggttt ccgtgccgca tttgaagata
     4261 aagggcgctt taaagaatat gtctatgata ttccggtgta tctcatcgtc catgacaatc
     4321 cgggtcttct cggttccggt gcacatttac gccagacctt aggtcacatt ctgtaaatcc
     4381 ttcctgttat atcgggaggt aactctcccg ataatctttt aaatcataca gttaattcaa
     4441 tttttctttg tgtcccctca caagaccgac ctgcgtcaca cttccgtaca gcgggattaa
     4501 ttctccagta aatgcattat ttgtctggta acggcgattt gttttgcacg ttcataattt
     4561 cactcgtcaa ctatcactga atgaggaatt aacgatgagt aagaaactaa ttgccttatg
     4621 tgcctgccca atgggcctgg ctcacacttt tatggccgct caggcgctgg aagaagcggc
     4681 ggtagaagcc ggttatgaag tgaaaattga aacccagggc gcggacggta tccagaatcg
     4741 cctgacggcg caggatatcg ccgaagcaac catcatcatc cactccgtgg cagttacccc
     4801 ggaagataat gagcgtttcg aatcacgcga cgtttatgaa atcactttgc aggacgcaat
     4861 taaaaacgct gcgggcatca tcaaagaaat cgaagagatg attgcctctg aacaacagta
     4921 atattcgaca gggataattg ttatggccat taaaaaacgc agtgcaaccg ttgtgcctgg
     4981 cgcatccggt gcggcagcgg cagttaagaa tccgcaggcc tctaaaagca gcttctgggg
     5041 tgaactcccg cagcatgtga tgtcagggat ttcacgcatg gtgccgacct taattatggg
     5101 cggtgtgatc ctcgctttca gccagctgat tgcttatagc tggcttaaaa ttcctgctga
     5161 gatcggcatc atggatgctc ttaatagcgg gaaattctcc ggtttcgacc tctccttact
     5221 gaaatttgcc tggctgtcgc agtccttcgg cggcgtgttg tttggttttg ccattccgat
     5281 gtttgctgct tttgtggcga actctatcgg tggcaaactg gcgttcccgg ctggttttat
     5341 cggcggcttg atgtctaccc agccgacgca actgctgaac ttcgatccca gcactatgca
     5401 atgggcgacc tcttcgccgg tgccgtccac cttcattggc gcgctgatta tttctatcgt
     5461 tgccggttac cttgtgaaat ggatgaacca gaaaatccag ttaccggatt tcctgttagc
     5521 gttcaaaacc acatttttgc taccgattct ttccgccatt tttgtcatgc tggcgatgta
     5581 ctacgtcatc accccgtttg gcggctggat caacggcggt atccgtactg tgctgactgc
     5641 cgcaggtgag aagggcgcgc tgatgtacgc aatggggatc gccgcggcga cagcaatcga
     5701 ccttggtggc ccgatcaaca aagccgcagg tttcgttgcc ttcagcttta ccactgacca
     5761 cgtattaccg gtcaccgctc gttctatcgc tatcgttatt cctccgattg gtctgggtct
     5821 ggcgaccatt attgaccgtc gtttaaccgg caaacgcctg ttcaacgctc aactttatcc
     5881 gcagggtaag accgcgatgt tccttgcctt tatggggatc agtgagggtg cgattccgtt
     5941 tgcgctggaa agccccatca ccgccattcc gtcgtatatg gtcggcgcta ttgtcggctc
     6001 aaccgccgct gtctggctgg gcgcagtgca atggttcccg gagtccgcta tctgggcatg
     6061 gccgctggtc actaacctgg gcgtctatat ggcggggatc gcgctggggg cagtcataac
     6121 tgcgctgatg gtggtgttcc tgcgtctgat gatgttccgt aaaggcaaat tgttaatcga
     6181 tagcctgtaa gaaggaaaat ccagatgaca ttactcgctt cgctgcgcga ctggcttaag
     6241 gcgcaacaac tggatgcagt gcttctctcc tcacggcaga acaaacagcc gcatctgggg
     6301 atctccaccg gatcaggtta tgtggtgatt agccgtgaaa gtgcgcacat tctggtggat
     6361 tcgcgctatt acgttgaggt ggaagcccgt gcgcaaggct accagctgca tttgcttgac
     6421 gcgacgaaca cgcttaccac tatcgtcaat caaatcattg ccgatgagca gttgcaaacg
     6481 ctcggttttg aaggccagca ggtgagttgg gaaaccgcgc accgctggaa gtctgaactc
     6541 aatgcgaaac tggttagcgc cacgccggat gtgctgcggc aaatcaaaac gccagaggag
     6601 gtggagataa tccgcctcgc ctgtgggatt gccgatcgcg gagcggagca tattcgccgc
     6661 tttattcagg cggggatgag cgagcgcgag atagccgctg aactggagtg gtttatgcgc
     6721 cagcagggcg cagaaaaagc ctcttttgac accattgtcg ccagcggctg gcgcggggcg
     6781 ctgccgcacg gcaaagccag cgacaagatt gttgcagcgg gcgagtttgt cactctcgat
     6841 tttggtgcgc tgtatcaggg ctactgctct gatatgacgc gcaccttgct ggtgaatggc
     6901 gaaggggtga gcgccgaatc tcacccgctg tttgacgtct atcagattgt cctgcaggca
     6961 cagctcgcgg caatctctgc aattcgcccc ggcgtgcgct gccagcagat tgacgacgcc
     7021 gcgcgccggg tcattaccga ggctggattt ggcgactatt tcggtcataa cactggtcac
     7081 gctatcggca ttgaagtcca tgaagatccg cgtttttcac cgcgggacac cacgacgcta
     7141 cagccaggca tgttgctgac cgtggagccg gggatttatt tgccagggca agggggcgtg
     7201 cgcatcgaag atgttgtgct ggttaccccg caaggcgcag aagtgctcta cgccatgccg
     7261 aaaacagtgt tgctcacggg agaggcataa tggatttatc gctattaaaa gcgttgagcg
     7321 aggcagatgc gatcgcctcc tcggaacagg aagtgcggca gatcctactg gaagaagcgg
     7381 atcgcctgca aaaagaagtg cgatttgatg gtctgggatc ggtgctgatc cgcctcaatg
     7441 aatcgacagg tccgaaggtg atgatctgcg cccatatgga cgaagtggga tttatggtgc
     7501 gcagcatctc ccgcgaaggc gctattgacg tgctgccggt tggcaacgtg cgcatggctg
     7561 cccgccagct gcagccggtg cgcatcacca cccgtgaaga gtgcaaaatt ccaggcctgc
     7621 ttgacggcga ccggcagggg aatgacgtca gcgccatgcg cgtggacatt ggtgcgcgct
     7681 cctatgacga agtgatgcag gcgggaattc gtcctggcga tcgcgtcacc tttgatacaa
     7741 cttttcaggt tctccctcac cagcgggtga tggggaaagc ctttgatgac cgcctcggtt
     7801 gctatctgct ggtgacgtta ctacgtgagc tgcacgacgc cgaactgcct gcggaggtgt
     7861 ggctggtggc aagttccagc gaagaggtgg gattacgcgg cgggcaaact gccacccgcg
     7921 cggtgtcgcc ggatgtcgcc attgtgcttg ataccgcctg ctgggcgaaa aactttgatt
     7981 atagcgcggc taaccatcgc cagattggta acgggccgat gctggtgtta agcgacaagt
     8041 cgctgattgc gccgccaaaa cttaccgcct ggatcgaaac cgtggcggca gaaattggcg
     8101 tgccgttgca ggcggatatg ttcagcaacg gcggcacgga cggcggagcg gtgcatttaa
     8161 ccggcaccgg cgtgcccacg gtggtgatgg ggccagcgac gcgccacgga cattgtgcgg
     8221 catcgattgc cgattgccgc gacattttgc agatgcagca acttttatct gcccttattc
     8281 aacgtcttac ccgtgagacg gttgttcaac tgacggattt cagatgatct cctgattaac
     8341 ccggagcggt tatgttaacg attcaatttc tctgtccttt gcctaacggt ctgcatgctc
     8401 gtccggcgtg ggaacttaaa gaacagtgca gccagtggca aagtgaagtc acctttatta
     8461 accatcgcca gaacgcaaag gccgatgcga aaagttcgct ggcgctgatt ggcaccggca
     8521 ccctatttaa tgacagttgc agcctgaaca ttagcggcag cgatgaagag caggcgcggc
     8581 gcgtgctgga agagtacatc caggtgcgct ttatcgacag cgacagcgtc cagcctacgc
     8641 aggcagaact gacggcgcat ccgctgccgc gttcattaag ccgccttaac ccggatttac
     8701 tgtacggcaa tgtgctggca agcggcgtcg gcgtgggtac gctgaccctg ttacaaagcg
     8761 acagcctcga cagttatcgg gcaatccccg ccagtgtgca agattccacc cggctggagc
     8821 acagcctggc aacgcttgcc gagcaactga accagcaatt gcgtgagcgt gacggcgaaa
     8881 gcaaaactat cctcagcgcc catttgtcgc tgattcagga tgatgaattt gcaggcaata
     8941 tccgtcgcct gatgacagaa cagcatcagg gactgggggc ggcgatcatc agcaatatgg
     9001 agcaggtttg cgccaagctt tctgcttctg ccagcgatta tctgcgcgaa cgtgttagcg
     9061 acattcgcga tattagcgaa cagttgctgc atatcacctg gccggaactg aagccgcgca
     9121 acaatctggt gctggaaaaa ccgaccattc tggtggctga agatttaacc ccaagccagt
     9181 ttttgagcct tgatttgaaa aatcttgcgg gcatgattct ggagaaaacc gggcgcacct
     9241 cgcataccct gattctggcc cgcgcctcgg cgatcccggt actgagcggc ctgccgctgg
     9301 atgcgattgc ccgttatgcc gggcaaccgg cggtgcttga cgcccagtgc ggcgtgctgg
     9361 cgattaaccc gaatgacgcg gtgagcggtt attatcaggt tgcgcagacg ctggcggata
     9421 aacgccaaaa acaacaggcg caggctgccg cgcagctggc ctattcccgt gataacaagc
     9481 gtattgatat cgcggcgaat attggcaccg ctctggaagc accaggcgcg tttgccaacg
     9541 gcgcggaagg cgtcgggctg ttccgtaccg aaatgctgta tatggatcgt gacagcgcgc
     9601 cggacgagca ggagcaattt gaagcctacc agcaggtgct actggcagcg ggcgacaagc
     9661 cgattatctt ccgcacaatg gacatcggag gcgataaaag cattccttat ctgaatattc
     9721 cccaggaaga gaacccgttc ctcggctatc gcgcggtacg tatttacccg gaatttgctg
     9781 gcctgttccg cactcaacta cgggcgattt tgcgcgctgc cagttttggc aacgcccagt
     9841 tgatgatccc gatggttcac agcctcgatc agatcttatg ggtgaagggc gagatccaaa
     9901 aagcgatcgt tgagcttaag cgcgatggcc tgcgtcatgc agagacgatt actctgggta
     9961 tcatggtgga agtgccgtcg gtgtgttaca tcatcgacca cttctgcgat gaggtcgatt
    10021 tcttcagtat cggttctaac gatatgaccc agtatctgta tgcggtcgat cgtaataacc
    10081 cgcgcgtatc gccgctgtat aacccgatta cgccatcgtt cctgcgcatg ttgcagcaga
    10141 ttgttaccac tgcgcatcag cggggcaaat gggtaggcat ttgcggtgaa ctgggcggtg
    10201 aaagccgtta tctgccgcta ctgcttgggc tgggcctgga tgagctgagt atgagtagcc
    10261 cgcgtattcc ggcggtgaaa agccagcttc gtcaactgga tagcgaggcg tgtcgggaac
    10321 tggcgcgtca ggcatgtgaa tgccgcagtg cgcaggaaat tgaagcgtta ctcaccgcct
    10381 ttacgccgga agaagacgtt cgcccactgc tggcgctgga gaatatcttt gttgatcagg
    10441 attttagcaa taaagagcag gcgatccagt tcctgtgcgg caacctcggc gttaacgggc
    10501 gcactgaaca tccgtttgag ctggaagaag atgtctggca gcgggaagag attgttacca
    10561 ccggcgttgg ttttggcgta gcgatcccgc acaccaaatc tcagtggatc cgtcattcca
    10621 gtatcagcat tgcccggctg gcgaaaccga ttgactggca gtcagaaatg ggcgaagtcg
    10681 aactggtgat catgctgacg ctgggtgcta acgaagggat gaatcatgtg aaagtcttct
    10741 cgcagctggc gcgtaaactg gtgaataaaa acttccgcca gtcgttgttt gccgcgcaag
    10801 atgcacaaag tatcctgacg ctgctggaaa cagaattaac cttctgacgt tagccctgaa
    10861 aacgggcgct gtactctccc ggcgtcagac caaactgacg ccggaaaacg cgacaaaaat
    10921 agtcgctatc cggaaaaccg caacgctgtg ccacttcatg aattgacaga tgatatttct
    10981 gcaaaatcat cctcgccttc gccattcgca cccaacgcac atactcgata aaacgcatcg
    11041 ttccatgctg agcaaacagt tttgacagat gattgggcgt gatattaaaa aactgcgcca
    11101 cgctctcgcg ggtgagcggc tgggcataat tatcctgaac ccagttacaa atgctgtgat
    11161 agagaaaatc gccgcgcggt tgcgaattac ccggcggcat attcaccacg ttgcggcaaa
    11221 gatgcagcaa acttaacacc agtggctgaa tgatattctg ctccagcggc gaacggctaa
    11281 gatgggtgag ggcggtaagc atcgcttcac cttcaccgcg ttgcaggctg ggaagctgga
    11341 tctggcgagc aggatgcaac aacgacgtgg tgcggctatc gtaaaacgat aacccaagcc
    11401 aggtcggtgc gaacaccagg cttaacagca tcaccggttt gttgttgatc ggtaaattag
    11461 ccgcgcgtgc cggaataaac agcatttctc cttccaccat tcgctgctgc tttgcttcca
    11521 gcaggttgcc gtattcgccg cgcagcacga tatccagacg gggaaaatca atacataaac
    11581 tgcccaccgg taacgaggca ggctggctgg caaaccagac gcgccccagt agttgcgggt
    11641 taagcaccag gccgctgaac agatcggcga aaaattgttg atcggcgggc aagcctggtg
    11701 ctttcatgaa agttcccgat taaagatgca ttaactggcg aaattctttc actttgctgc
    11761 gactgaccgg cacttcaaaa tccagatctt tcagtcgcag aatgtaggtg ttattaaacc
    11821 acggttcgat ttcgcgtatt ttgttcagat tgacacaaaa tgagcgatgg cagcggaaaa
    11881 aatgcgacgg cggcagtttg ctgcaaaatt cggtaatgtt catcggcatt acgtaggatt
    11941 cacgccgcgt atagacaaag gtcattttct cgtgcgcttc ggcgtaatag atatcgttaa
    12001 ttggcgtgac gattattcgc tcatctttca ccagattaat ggtgtcattc tcacgcgtta
    12061 ccgtcgcggc aggcgtactg ctggtctgct gttgttgcca ggccgcttcc agtttttgca
    12121 gcatcccggt aatacgtgac tcctgatacg gtttgagaat gtagtcaaac gcctccagtt
    12181 caaacgcttc taccgcatgt tctttccacg cggtgatgaa cacaataaac ggtttatggg
    12241 cgaactggct gatgttttgc gccagcaaca cgccatccag cgacggaata ttgatatcca
    12301 gaaaaatggc gtcgacgcgg ttatgctgca aaaacttcaa cacgtccaga ccgtcgtcaa
    12361 aggtgccgac aatctccatc tggctgtgct ctttaattag ccagctcagt tcctgttgtg
    12421 ccaggaattc gtcttcaaca atgatgactt tcacaatatc actccggctc aaagcaataa
    12481 cgtagcctgt gaggcgactg gggtgcgttg gttaggaatg taaaacgcaa tttccgtccc
    12541 cggctccagg cggcggatat gcagcccctc gccatacaat aacttcacgc gatgatggac
    12601 attcagcagg ccaattttat tgcccggcat ttcattcgct tcgacccgct caatcacctt
    12661 tggatcgatg ccgtgcccgg tatctcgcac cgcaatgcgt acccgatttc cgcactctgc
    12721 aacgctgatg gtgacaacgc ctttaccttt gcaaggctga ataccgtgga caatggcgtt
    12781 ctccaccaac ggctggatca gcaggctggg aatgcagcaa ttcacctctt catcaatatc
    12841 atagataacc gtcagcttgt caccaaagcg ggcctgctca atggcaatat agtctttaat
    12901 ttgatacagc tcttttttga tatcgatttg ctcatcgtct tttaattcaa tgttatagcg
    12961 cagataacgc gacagattaa agatcagctg gcgagcagta tccggattca gacggattga
    13021 tgatgaaata gcgttcaaag cgttaaacag gaaatgggga ttaattttgc tttgcagggc
    13081 gcgaagctct gccttatttg ccatttcacg tagctgctcg gcgcgtgaaa cctccagttg
    13141 ggtggagatg atttgcgaca gaccgaccgc catttcctgt aatgacgagg tgatctgatg
    13201 cgcgtggcag tagtaaattt tcagcgttcc ggtcacaacg cctttctccc acaatgggat
    13261 caccagcatg gaatgaatct ctggtgtgcg gtgggcttca tcattgtttt taatgatgat
    13321 ttttccgtaa ttcatcgcct gacgggtagt cgggctaatg aagtcatcgc cattctgata
    13381 gttatgttca cccacgccaa cataggccag cacatgatcg gtattggtaa tcgccaccgc
    13441 atcggcgtga atgtcatcgc gaataatttc acaaactttg cgtaatgact cgctattgac
    13501 atggcgaaac agaggtagcg ttttgttggc gatatccagc gccagcttgg cctgccgcgc
    13561 ggcgctggcc tctttttcgc cctcaacgct ttgcaccaga agcacaataa agccgataca
    13621 gacgctaccg aggatcattg gaatgccgat tttagagacg atatcgatcc ccaacgcggt
    13681 agttggtgcc cagacaatta ccagaatcat ggtcaacgtc tcacacaaca tgccgccgag
    13741 aataccgacg cgccagcgct gtgctttggg gattttcagg tttatccagc ctgatataca
    13801 acccgccaga atgctggtga taaagcaggg gatcgccgtc acgccgccga tatcaattaa
    13861 ataccggtga atacccgcaa tcacgccggt aatgatgcct acccacgggc caaacagaat
    13921 cccgccggac atcaccgcga taatacgtac gttcaccagc gagccttcta cgggaacgcc
    13981 ggaccaggtg ctgaacaggg cgaacagcga gaaaatggcg gtaacggcga gcaattcttt
    14041 tggggagtgc gccgacttgt gcaacagttc gcgaaacagg cggatacgga tgagaaagaa
    14101 caggcagata agcattaacg ctgcccgatc gaagaccgcc agcagcatgt tgaatatttc
    14161 gtgcacgggt gactctgaat aaggattaaa cctctatgat aggtaacctg aaggctgatg
    14221 accagcaggc cgtttttgag gaaaaaccat aaatttaaaa ggggattgaa agataaatgg
    14281 tgtattagca atgtatctct tgataatgca cttctggctg g
//
LOCUS       DAFAVF010000106        14308 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFAVF010000106 DAFAVF010000000
VERSION     DAFAVF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.106"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(221..1603)
                     /locus_tag="LGK42_003124"
     CDS             complement(221..1603)
                     /locus_tag="LGK42_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK2659348.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(1613..2563)
                     /locus_tag="LGK42_003125"
     CDS             complement(1613..2563)
                     /locus_tag="LGK42_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBK2659349.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(2706..4124)
                     /locus_tag="LGK42_003126"
     CDS             complement(2706..4124)
                     /locus_tag="LGK42_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBK2659350.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4124..5671)
                     /gene="fdrA"
                     /locus_tag="LGK42_003127"
     CDS             complement(4124..5671)
                     /gene="fdrA"
                     /locus_tag="LGK42_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBK2659351.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(5661..6524)
                     /locus_tag="LGK42_003128"
     CDS             complement(5661..6524)
                     /locus_tag="LGK42_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBK2659352.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHHFIHDIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(6564..7169)
                     /locus_tag="LGK42_003129"
     CDS             complement(6564..7169)
                     /locus_tag="LGK42_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBK2659353.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7427..7924
                     /locus_tag="LGK42_003130"
     CDS             7427..7924
                     /locus_tag="LGK42_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBK2659354.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8016..8948
                     /locus_tag="LGK42_003131"
     CDS             8016..8948
                     /locus_tag="LGK42_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659355.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAADELGLTTSAISYTIKRME
                     TGLDVVLFTRNTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFMPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(8990..10078)
                     /locus_tag="LGK42_003132"
     CDS             complement(8990..10078)
                     /locus_tag="LGK42_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2659356.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10220..14203)
                     /gene="ehaA"
                     /locus_tag="LGK42_003133"
     CDS             complement(10220..14203)
                     /gene="ehaA"
                     /locus_tag="LGK42_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HBK2659357.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVTIEKNGAL
                     GITQNIGSAEITNRGKLHLKAEDSMTFANKISGNGTISIDSGTVELTGNNYAFYGYID
                     VASDAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALAAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKISGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGIGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGRNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3245 a         3926 c         3404 g         3733 t
ORIGIN      
        1 ttttcgatac tgaatctttt ttatccgaag caggtcacat ttatggcctg ataaatacct
       61 ttgttaaaac tcacttttgt gtatttctct caaataatcc cctggaaatt atgccgcgag
      121 attataaagt tcaggatcat tcattagcaa accattcccg ccccactccc tggggcttaa
      181 atctcttaca attcaccgtt gcaggcgttt caaatgcgca tcaacctgcc acactccccc
      241 acaccagttg ccctttatgg aacgttgcgg ttctcggcga gatacgcgcc accgcctcgg
      301 cggaacagga ggcgtccacc agcacaaagc tggcatcatc ctgcgctttt ggccatacac
      361 gctcgccttt ttcattcagc ggcaatacat cgccagtggc tagaaacagc gaacgggaga
      421 ggttctgttc gttaggacga atatagagct gcgcgtagag attggctttt tccagcatgt
      481 cgcccagacc ataaggcgac cagtggtcga taacgctgtc agtgccagtc atcactttta
      541 cgcctttgtc gtgcaactgt ttgagcggca tatgcagcgt gccaatcggc accgtcgagg
      601 cgatagaaat ttgctgcgcc gccatccgat tcgccagcgc atcgacctgt tgttcgttga
      661 gcgtagccag cgcaaaggcg tggctgatgg tcaacttacc tttcagttgc ggcgttttct
      721 ctaccgtttc aaccatataa ttgatggctg ccacgcccga cggagtggtt tcgtgcaggt
      781 gaatatcgac acctttgtcg tagtccagcg caatctgaaa catggtgtcg agggattttt
      841 ccatcgcgcc atcaacactg gtcgggtcca gcccgccgac gtaatgcgca cccgcctgca
      901 tcgcttcgcg cattaagggt tccgatttcg acagcagcaa accgtgctgc gggaaggcga
      961 cgatttcaca ctcgaagccc gcctgacgtc gcgccagcac cgcctgcaaa ttctgcagat
     1021 ttttcaggcc ggaaaccggt tcgatattgc agtggctgcg ggcaatggtg gtgcctttcg
     1081 actgcaatag atcgatcagt ttttccgccc gctcctgcgt atacggttgt aactccggca
     1141 gcattttctg ctcgagtttg atcatgtcct ggatagtggt gcctgccgga cgattgagcg
     1201 agcgccacgg cccgccgtag aaggttttgt cgagatgaat atgcatgtcg cgagtggtgg
     1261 gtagcatcaa cttaccgcca gcgtcatagt gcggcagcgt ggcgtccgga tgctgcttgt
     1321 tctcgcgcag ggcgacaatt ttgccgtcct gaatctccac ggtctggcgc gccgtgcggg
     1381 tctgcaccgc cacaccgttt tcgtagtcaa aaccggtttc cagcagcacg ttatcgagat
     1441 agtaatgcgg gtcagtgatt ttcatggtgt tgttcgcttc gcagtttagg gtgccagcta
     1501 ccgccgcatg ggcgagcggc acagaggtac caaacagcgc ggcggcggtg accatcttac
     1561 cgctctggct cagaaattca cggcggctat tgctttcttt catcctacgt ccttacgttt
     1621 taataatatg ggtgcccgtt tcaccgcgca gcgccgcagg caggcattcc ggcgtggtga
     1681 taatcacttc tttgccgccc tgctccagga acgtcaggct ggcgatgatt tttggcaaca
     1741 tgctgccggg cggaaaatgc ccttcctgca tatagcgagt catggtggca atatccaccc
     1801 gatccagcgc ctgctgctgc ggtttaccaa agtggataca gactttttcg acgccggtgg
     1861 tgatcaccag aatgtcggcg tgaatttcac gggccagcag cgcggtagag agatctttgt
     1921 caataaccgc gtccacgctt tggtaatccc ccgcttcagt acgcactacc ggaattccac
     1981 cgccgcccgc gccaattacg acaaaacctt gttggatcag cgccttaatg gcgggtgctt
     2041 cgacaatacg tttcggttcc ggcgaggcga ccacgcggcg atagccccgt ccggcatctt
     2101 caacaaaaca ccagtcaggg tttgcctttt gtagtttgtc acgctggctt tcactgaaga
     2161 atgcaccaat gggcttggtg ggatgggcaa aacctggatc gtttttatcc acttccacct
     2221 gagttaccac ggtgacggct tttttctcgc cgtgacgcgc cagccggtta ttcagcgcct
     2281 gttggatcag atagccgatg ccgccctgcg tatctgccac acagttcgcc agcggcgtta
     2341 agggcagccc ttcgcgctcg tgggcaatct ccgcacggcg taaatccagc ccgacctgcg
     2401 gcccgttacc gtgggtcagc acaatgtcgt aatcggaagc cagcatttcc agcaccgtat
     2461 cggccacggc tttcaccgcc tccgcctgat gctcaatcga ctggctggcg ttatctttga
     2521 taatgctgtt gccaccaatg gcaacgacca caagctcttt catgttgtta tgtccgctac
     2581 agagggtgat taggttatga ccaacgccag actgcctgat gcgctacgct tatcaggcct
     2641 acaattctcg ggccatccgt aggccggata aggcgttcac accgcatccg gcataaacat
     2701 gtaagtcaga cgccgtaacg ttcgcaccag ccgagaatgg ctttctcgaa gcaggcgaat
     2761 ggtggatgca caatgcccgc gccaatcatg ccgacgcccg catctttatg ggcgatggcg
     2821 gtgttgatca ccggcagaat gccgctgcta cccacgcggg tgatgtcgat tgccgacggc
     2881 acgcccataa aaccgagcag cggaatggtg acgttggggt tttcgccaag ggtgatttcg
     2941 cgcatctgac gggagaaatc gatagcctct tccaccgtgc cgcccaccag tgcgacgatt
     3001 gcaggcgcgg tcgccatggc aaacccgcca ataccgtagg tttctgtgat agcgctgtcg
     3061 ccgatatcca gccccgaatc ttccggctta taaccagcaa acatcgggcc gatgacctgc
     3121 tgcgctgggc cggtaaacca ctgccccggc aggccgctga cccgcaggcc gaactcgacg
     3181 ccgttacgcg ccatggtggt gaccacggtg ctgtattcga tgccgtgcgc cgcatccatc
     3241 gccgctttac acatcgccat ccacgtcggg ccggagaagt agtcgctgct ggcgacaaac
     3301 tcgaacactt cgcgctgttg ctcgacggaa tagcctgcct gaatgatccc cggcgtcagt
     3361 gcctgaatca gcagcgtcgt cccggcgtta ttgcggttat ggcactcatc gcccatatgc
     3421 agcgcctgcg ccagcattaa gcgcagatcg atctcaccga taattttcat cgcgtcgcgc
     3481 agcattggcc cctgcacatc gcgcatccag ttgaggcggt cgatcacgct ctgatcgttg
     3541 gcacccatac gcaaaatctt cgccatctgc tcgctcatat tggtataagc gatgttgccg
     3601 taggttttgt ttttcacgat gtgcataaac atcgaggccg aggtaacgcc cgccatcgat
     3661 cccacgcagt cgtgttcgtg gcacggcgag aaggtgatct ccccggaggc cgccagttca
     3721 gtcgcctcat cgagatcttt cgccagccct tcgaacacca acgccccggt gaccgcgcct
     3781 ttcatcgcgc cgcacatttt ttcccaggtg atcggcggcc cggcgtgaag aatggttttc
     3841 ggcgtcatgc ccggcaccac gttaatcgcc tgatcaaaac caatcagcac cggatgcgac
     3901 tggataatgc gctccagcgc ctgctggtta gcggcggcga ttttgtctgc cagcggcgaa
     3961 tcggcaatgt tatccagcgc ctgcaccacc tgcatattgc cctgccccgg cggcgtccag
     4021 tcgagttgag tcacttctac atgctgcttt ttcaggtcat cgctaaacat ggcgataccg
     4081 acgttaataa cgttcaacgg ttggctaaac agtgactggc tcatcaggct tcctcccctt
     4141 tgcagataaa ttcacgcgcc agcaatccgg tattggtgct gctgctcgcc agaatcactc
     4201 cggcatcaag cagcatctgg ctttgttgtt ctaatgatgg cgtatcgaga tcggtgccca
     4261 gcacataggc gagaatgatc aactcgcgtc cttcggcggc ggcgatcgct ttcgcttctt
     4321 tgatcgcctc gatggtggag ccgaccggat cttcatgcga tccaaatccg agcacaaaat
     4381 ccatcacgat caccgccact tctggatcgc gcgcttcttc gatcaagcga ctgatgcggt
     4441 tggtggggtc aatcattgga tgcggcttgc cgttggtgaa gtcgtcatcg ccaaagtcga
     4501 ggaaggtgtg tttgatgctg cggttgatat cttgcaggcg gaattcagga tcgggctgaa
     4561 tgttgctgta gacatcgcca tgtttttcca tcaccgcgaa catggtttcg tcgcacagcg
     4621 tgccgccgca gaacaggcca cgtatgtatt tctgctgcgg ttgcagacgc gcacgcacat
     4681 cagcaatcaa cggctggtta agcgtatgca ggtcgagatt ttcctgtttc acgccggaga
     4741 gcatcaccgc ttttagcgcc gcctctttgc tgccgcgggc aaactgtagc ccctgctcat
     4801 ccaccggcgt ttcaacgcga ccgaggaagc agaccaccac cggcttgcgg caggcgcgcg
     4861 cgcgttccag cactttacgg gccaccgcag gcgcgggcgg tttggagata agcacaatga
     4921 tttcagtttg cggatcgttt tccagcatcc cgatggcgtc gagcatcatc aggccgccga
     4981 ttttctcgct caggtcgcgc ccgccggtgc caatcagttg tgaaacgccg ccaccaaatt
     5041 catgaatgcg gacgctcagt tcctggctgc cggtgccaga tgcgccaaca ataccgatgt
     5101 tgccacgacg cacggcgttg ccaaaacaga gcgccgcgcc gttaataatc gccgtaccgc
     5161 agtctggccc catcatcagc agcccttttt cgtgggccag ttgcttgagc gccagttcat
     5221 cttcaactga gacgttatcg gaaaacagca tcacgttgag atcgttttgc agcgcctgac
     5281 gcgcttcgcg agcggcaaac agaccgttga ccgaaatcac cgccaggtta ctttccggga
     5341 tatgcttttt ggcgctggca atagtggcgt aacgcgcctc gtgcgagccg ctttgcgctt
     5401 tgctattgaa cagttcttca atctccgcca gcaactgctc attgtccgca cccgatttac
     5461 cattgatgac aatcatcagg tcgccgtttt tcgcctgttc cagctccggc gtcagcagtc
     5521 ctaaattttt cagcacacct ttgttcattt cggtcgccat cgccacaaat gcctgctcga
     5581 cgccgtcgag tttatttgca cgcgtggaga tagacatcag cgagacagaa tcaaaatagg
     5641 tattcggttt aatgactatt ttaactgaca taattccctc cgtaggtttg gctcagcgca
     5701 caaccatcgg ccatgccata cagcatgtcg cagccggaac tggagccaat atgtttaatt
     5761 ttttctattg cctgagttga attcccaggg acgccataaa taatatcgtg aataaaatga
     5821 tgaatatttt cccggcagcg ttgttgtaat gcggcttcca gcgttatggc acttaataat
     5881 gtggtattat ttctgccgcg ttgcagaccg cgataaaatt cctctttgta tttttccgcc
     5941 ggatgcccgg aaataaataa aataagtacc agtccggtca gataatcatc ggctgatggc
     6001 gtaaggccaa tccctaaccc catcatactg gcgaccgctg cggcgatatt ttgtttttcg
     6061 tttactgcct gaataactgc ccgtcgttta atatgtagtt ctttatttaa cgcctgataa
     6121 aacggattat cgccgcgata attaaacagg gtttcatttt cccccaggtg ctgatgaata
     6181 atgtcgtgcc actgttgcca ggcgataagg tgaaaattca cagcggtcat ttgcaaggtt
     6241 ggggacagcc aacgccgaca acgactcatc tctatatgaa gatgttgacc aaccgtaata
     6301 ccttgatcgt caaactgaac cttatcacca tgccggaaca gatcatcaaa gtgagtgagt
     6361 gccaaccgac aactgtttgg cgcattgtcg tactcttcac aaagcagcgt cagcaactgc
     6421 tggcgagccg ggagtgcgat atttaccgct ctggcaaaga cctgttcaac ctgtccttgc
     6481 ccccgctgcg ccagaaaatc cgcatccgca gttagcgccc acacacaccg tctttctggt
     6541 gtgttgatag caagcctccc ggtttatgca cctgcggcaa tcagtaactg cgcaatctct
     6601 tcaaagcccc gttcccgcgc cagttccagt ggcgttttgc catatttatc ggtcagatgc
     6661 gggctggcac cgtgttccag caataactgc acaatcgcct gctgtttaat accaccatca
     6721 ttaagcacaa tcgcttccag tagcggcgtc cagcctacat ggttggtctg gttaacgtta
     6781 atctccgtat gcgccaaaag ctcttttaca atacttaaat ggcctttttc acaggcaggc
     6841 gtcaggccga caccgccaaa acgggttacg caattaagat cgggtttagc cggtaaaata
     6901 attcgtagta gcgttaaatc atcgttcaga cagctaatta aaaaaggatt taaacaggta
     6961 tgatcttgct tattaatatc cgctccggca tcaattaatg cctgaacgca agcatattgc
     7021 tgatataaac ttgccagcgt aattgccgtt ttcccctgac gatcgcaggt attaatatcg
     7081 acacccagcg caaggcaggt ttttacttta tcgatatcac cctgttgtgc agccaataaa
     7141 tagtcggcag gtagattttt tatactcatt cacattcccc tggtttttgt ggctgttttc
     7201 gatgaattga gaatagcaac gataatctga taaaagaacc cgcttaaatt taattcgctg
     7261 tatttcgcct ttttgttgaa tgatgttcaa caatcttttc gcagtgtgcg gtagtgcaat
     7321 atttgtttct ttgacccgat accctttcac tcaatcaggt tttatttctc tatagtctgc
     7381 acatgtaaga gtagctttcg tccatgtcta accagagggg aacaagatga acggactcac
     7441 ggcaacgggt gtcaccgtcg gtatttgtgc agggctttgg cagttagtgt cttcacatgt
     7501 cgggctctct cagggatggg aattattagg aaccattggc ttcgtcgcct tttgcagttt
     7561 ttatgccgca ggcggcggta aatcggggtt tatcagaagt ctggcggtta attattccgg
     7621 gatggtgtgg gcatttttcg ccgcgctggc tgccggttgg ctggcctctg taagtggact
     7681 gtccgcgttc tgggcgagcg taattactac cgtacccttc tcggcggtag tggtctggca
     7741 agggcgtttc tggttactgt cctttattcc cggtggcttt ctcggcatga cgctgttttt
     7801 tgccagcggg atgaactgga cggtgacgtt actgggtttt ctggcgggta actgcgtggg
     7861 gataatttca gaatatggtg gacaaaaatt aagcgaagcg acgactaaac gcgacgggta
     7921 ttaaaagtta ttcaaaaatg attatctatc tttaaccgat acctctacag tcatagctgg
     7981 agaaattcat agtagataat ggcgagaatg gtttaatgaa ctcaattttt accgaagaga
     8041 atctgttggc atttactacc gctgcgcgtt ttggcagctt cagtaaagcc gcagacgagt
     8101 tgggtttaac cacttccgcc attagctaca ccatcaagcg tatggagacg gggctggatg
     8161 tggtgctgtt cactcgcaac acccgcagca ttgagttaac cgagtccggg cgttattttt
     8221 tccgcaaagc taccgacctg ctgaatgatt ttcacgccat caaacgcagt atcgatacta
     8281 tttcgcaggg catagaagca cgggtgcgca tttgtattaa tcagctgctt tatacgccca
     8341 aacacaccgc gcgcttatta caggtgctga aaaagcagtt tcctacctgc cagattaccg
     8401 tgacgaccga agtgtataac ggcgtctggg atgcgattat taataatcag gccaatatcg
     8461 ccatcggcgc acccgataca ctactggacg gcggcggtat tgattacact gaaattggcg
     8521 cgattcgctg ggcttttgct atcgccccgg atcacccgtt agcctttatg ccggaaccca
     8581 tcgccgaaag ccaactgcgt ctctacccta atattatggt ggaggacacc gcgcatacga
     8641 ttaataaaaa agtgggctgg ttgctgcacg ggcaggagtc aattctggtg ccagatttca
     8701 acaccaaatg tcagtgccag attttaggtg aaggaattgg ttttttgccg gattacatgg
     8761 tccgtgaggc gatgacgcaa tccctgctgg ttacccgaca aatccataat ccgcgccagg
     8821 attcgcgcat gttactggcg actcagcatt ctgctacggg tcaggtgacg cagtggatta
     8881 aaaaacaatt tgcgccaaat ggcatattaa cggggatcta tcaggattta ctgcatcggg
     8941 agaattaatc gctgccaacc tcccgatgcg ctgtgaatag cggtttacat caaccacctg
     9001 ctttcattac ccattcagag ataaatttat tacccggtac tggcggagag tacaaatagc
     9061 cctgcaaaaa gtgaacgcct ttaccgatca ttaaatccgc ctgctcctgg gtttctaccc
     9121 cttccgccac gatactcaga ccaggcttac gcgctagttc gacaatattg tccacaatat
     9181 gaccggagat ttcgtcgaca ctcgccattt gcacaaatga cttatcgatc ttaataaaat
     9241 cgaccgggaa cgcctgcaag taacgatagg tcgcataacc cgtaccaaag tcatccagcg
     9301 caaaggtaat gttgtgctga tgaaggctgt caaatatcgc tctggcttct ggcgttaccg
     9361 gaataggatt acgttccgtt agctcgagaa ccagcttgat tttatcgtta cctaatttct
     9421 taaccaggtt cagacactct ttttcaaatc ccgctgccaa aaaacaaccc gccgagacgt
     9481 tgatgccaat atggaaattg tccggcagca aatgttttac cggcatcaga atatccgcag
     9541 tctgtttcat cagttggcgg gtcattatga caataagacc ggatgactcc gccagaggaa
     9601 taaactgatc cggtgggata attcccgttt gtggatgttc ccagcggaca agtacctcac
     9661 agcccgtcag taccccagtc tgcgcgcaga aaaccggttg gatccacggt ttgaattcgt
     9721 ggttttccaa cgccagactg atggcttcag gcgtgacgat atggtgacaa cggttatcag
     9781 ggatataaaa attattttta tttcccgtgc cactgatgac ttgcggatga tattgaaaaa
     9841 agatatcccg ccagaaggta atatcactct ggatgcctag tttttcatac agctgcttct
     9901 tttgatgtga aattgtcttt gcactgcgat ttctgtactg tgagatttct gtaaccgaca
     9961 ttccctccat tagcagcttg agtaaaagcc gttctttttc ggacacgata ccaggcaacg
    10021 tcaaattcac cgttgccccg aactcttcca aaaaaacact caaatcatgc gaagccattg
    10081 aacacacctt gattaaatct tgtgcgccag ctaaatctgc gagtcttcac tctgactgat
    10141 aaagctggct gcaagaaaat gacacagaga gccgaattat ctcccgttcg gctctctctc
    10201 cttttactca ttattcagat cagaattgcc acttaatgcc aaccattgca gaggtgtcat
    10261 tatatcccct atcggcaacc tgaacgccca cattccccca gacattcagg ttggcattta
    10321 gctgtccttc caccccggtt tttatctcag caatatttcg ggctccatcc tgagtgacgg
    10381 acacgccatc catactggtg ctgaaatcct tactgttatg cagccagttc acttctacaa
    10441 acggctggaa ctcgcgggat ttaccgtcat ccattttatg gtggctcttc agccaggttt
    10501 ttacgccaag tcgggtttga acattgccat caccgttgct atgaacgagg gttccgttgc
    10561 tttcgcggtg cttatcggct ttgactccca tccaggtaac ctgtgcttgc ggctgaacat
    10621 accattcatt acgcgttccc tggctgccat taaattcagc taatttgtgt ttgtatccag
    10681 cttccagtga agcggtaaat ccttttgatt tataggattc actttgtaag tcatcccctt
    10741 tcactgtgtt atcaaaccag ctgtactgcg cccaactgtc gagatacgcg ccattacgcg
    10801 actcgtcatc ggcataccag gtggcataga ggccggtgct atatccgttc acactcgctt
    10861 ttgcacgata accggttcgc gaggaaatgg ttttgctgtc gctgttgcca tatcccgcca
    10921 tgaccccaac atgccagctg tcgctgccgt tttggctcca ctgcgcgaca tcgcctccca
    10981 gttgcagaac atagcgattg ctttgggttt tcagctggcc gctgccatca cgccatttat
    11041 tacgaccacc ttcatggcgc atccacatag tggtttgttt ctgctcaccc gtcaccatgt
    11101 cggtatagta cgtattaccc agacgctcat gcagacgcgt ggtgaacatt gtattcgctg
    11161 ctgcaaggtt cgcaatgtag ctacccgcct ccggtcgcag gtcattatcc acattcagat
    11221 ccggacccgg cgtaggtgtc gggttagggt tcggctctgg attcgggttt ggctccggat
    11281 tcggcatcgg gtctggctcc ggctgcagtt caggagaatc actgccgctg gtcagatacc
    11341 agttaccact atttgcccct tgtccacgcg cgagagtgta gtcatacgcc cccgcaacga
    11401 tacgaccagc ctgaacaaat tcgccctcag acttaccgtc tacatggata agttcgatac
    11461 cgttaagggt tttcgcacct ataccgccag cgttattcac cgtaacggca gtcgtgccgc
    11521 tagtatcgcc ctcgacaacc aatttatcgg ttactgagtc atcgccattc agtaccgtat
    11581 tcattactag ttgaccgtta ttaccgtggt agttcccctt caccactaac acattgccag
    11641 cttgctgtcc tgacattccg gtcatcagcg taccgttgtt ggtcagattg ccgcccaggg
    11701 tgtaattcac agacgcagcc gctctttttt cagtgctatt attcaacgcc agggtaccgt
    11761 tgttctcaac gtttgcgcta cccaatccct gcgcatgact taccaggaca ttagccgaat
    11821 tagcaatgtt cacattcgcc gtcaggttgc tgttatcgcc cgcgatgtcc agcgtgccgc
    11881 cctgaatatt cagctctccg ctccccgtta acgaaccggt tgaagtgccc ccgttggtaa
    11941 ccgtcagact gccgccattt aagctcagca gtgaatcggc gctgccattg agcgtgccca
    12001 cggtctggct gtgaccattc atatccagtt cagtctctgc cgccagacgc agttcatggg
    12061 tattacccaa cacgttatcg ttattcaaca aaagcgtccc actgcgcaga tcggtaacac
    12121 cggtatagtc gttgtctttg ttagaaagcg atacggtctg acctttctgg ctgctgaatg
    12181 ccaggtcacc actgccgcta atctttgcgc tgagatcggc ggcattctcg cttttaccgt
    12241 tagagctcag caccagagca tcgctgtcgg tagcgtgtaa atcaagctgg gtcaggccgt
    12301 aaccgatata cagaccgtca ttgtttaccc cactcgtcag acgatagtcg taagtgccga
    12361 cggccgcttt ttgcccattc tgctgaatat cacgttgggc gctgctggag aggatttgcc
    12421 catttttatc ctgcaattgc agattgcccg catcgcccag aacttccgca ccttgcgcat
    12481 caaccaactt aatggtgctg ttaccatcat caacttcagt aagcgataac gcggagtcaa
    12541 tatcacgaga tactgagcgg acaacgtcac tgtcagaaac ctgaatcaca ccctcgccgc
    12601 gcaaatccag cgttttacta acgttgactg tcccttctgt catctgtgca cctgctgcca
    12661 gggcaccaaa atcaaccgtt ccgccggaga atcccaaagt gctaatggag tgagcgccct
    12721 tacccactgt taccaggctt ccctgacccg cagtgatccc gcctttctgc agaaactcag
    12781 cattcttttc cagattaaat gtcgtgttct ggaacgccag tgaaccctgg aaccagtctg
    12841 tataagcaaa ctcatcaaag gagaattcgt gattccccat gtttatttca acaatgcctc
    12901 taccttcaag atcgttatca aatacccaat ctttattggc attgatttgc agtttgccat
    12961 caacatccag ttccgcacgt ccgatgttct tgtcttcaga aataacagct acagcatcgg
    13021 aagcaacatc aatatatccg tagaatgcat agttattgcc ggtcaactcc accgtcccac
    13081 tgtcgatact tattgtaccg ttgccggaga tcttattggc aaaggtcatg ctatcctcag
    13141 ccttcaggtg taatttcccg cggttggtga tttctgcaga gcctatattt tgggtgatcc
    13201 ccaaagcgcc atttttctca atcgttactt tgccgctaaa ggcagagtta tccccggtta
    13261 agtaagtcgt accattgagc gcattgatat agcccaggcc ctcgctgtca ctgacaatat
    13321 ccgcggaaat agtcgcgttt ttatccgtgt ggtttaaggt gattttcttc tcggatgtct
    13381 ttgcccacac aaactcaatt ttttcggcat caataatccc tgcagccttc gcagcacttc
    13441 cttcctgtgc acctaacgct aactcagagt tggtgcttaa actaatagtc ggcgcagaga
    13501 ttttaccacc ttccgaaaca gcaagtgttg tatcggagtt ttgatcataa taacccaggc
    13561 taattttctc actggcatta acttcaccgc cgttattaac tgacaggtac tgattaccac
    13621 cgtaactgcc gtaaacgccc ataaagaagg ttttagaatt tacctggctg ccttcaccac
    13681 tgacatttcc ctgcaaatag gtcagacggc tggtgccctt aacatggatt ttgccgccgt
    13741 tggaaacatt aaaatagccg cgatcgtgtc tcatgcctga gtttgattca tacaaatcat
    13801 cagcatataa ctcgctgcca gcattggaga cattcaggaa ccccgtccct tttaacaaaa
    13861 agttaagtgg gccattgatt gttacctttc ctccggtaat atccaaccca ccaccagaag
    13921 cgagagtcag acgcggacgg gaagtatctg tcgcattgat taccagattg gcattgtcag
    13981 tcaccgtaat acggtaatcg atgctctggt tctctttgcc aaaatcaata gttttatcct
    14041 ggtttacgat gacatcatcg gcgaatacca gagatgaata taaacttatc gcgctaatta
    14101 aaacagcccg ggactttttt ccttttactc tgctccccag ctccgagcag acgctccaac
    14161 agttacggga cctgttccac tttagccgat atattttatt catcacaatc tccagcaata
    14221 catccaaata aaaccaagat tgcataaaca gcaacgcatt aaatgccatt ccggttactg
    14281 ttcatcgcat ataaaaattc aaaaattt
//
LOCUS       DAFAVF010000107        14154 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFAVF010000107 DAFAVF010000000
VERSION     DAFAVF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.107"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..315
                     /gene="ecpR"
                     /locus_tag="LGK42_003134"
                     /gene_synonym="matA"
     CDS             <1..315
                     /gene="ecpR"
                     /locus_tag="LGK42_003134"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBK2659358.1"
                     /translation="IAARKSEALANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFL
                     RKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKL
                     VQ"
     gene            390..977
                     /gene="ecpA"
                     /locus_tag="LGK42_003135"
                     /gene_synonym="matB"
     CDS             390..977
                     /gene="ecpA"
                     /locus_tag="LGK42_003135"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBK2659359.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1035..1703
                     /gene="ecpB"
                     /locus_tag="LGK42_003136"
                     /gene_synonym="yagY"
     CDS             1035..1703
                     /gene="ecpB"
                     /locus_tag="LGK42_003136"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBK2659360.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1729..4254
                     /gene="ecpC"
                     /locus_tag="LGK42_003137"
                     /gene_synonym="yagX"
     CDS             1729..4254
                     /gene="ecpC"
                     /locus_tag="LGK42_003137"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HBK2659361.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4244..5887
                     /gene="ecpD"
                     /locus_tag="LGK42_003138"
     CDS             4244..5887
                     /gene="ecpD"
                     /locus_tag="LGK42_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBK2659362.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5856..6566
                     /gene="ecpE"
                     /locus_tag="LGK42_003139"
     CDS             5856..6566
                     /gene="ecpE"
                     /locus_tag="LGK42_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBK2659363.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6879..7208
                     /locus_tag="LGK42_003140"
     CDS             6879..7208
                     /locus_tag="LGK42_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBK2659364.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7456..8070)
                     /locus_tag="LGK42_003141"
     CDS             complement(7456..8070)
                     /locus_tag="LGK42_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBK2659365.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8488..9177
                     /gene="paoA"
                     /locus_tag="LGK42_003142"
     CDS             8488..9177
                     /gene="paoA"
                     /locus_tag="LGK42_003142"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HBK2659366.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9174..10130
                     /gene="paoB"
                     /locus_tag="LGK42_003143"
     CDS             9174..10130
                     /gene="paoB"
                     /locus_tag="LGK42_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HBK2659367.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10127..12325
                     /gene="paoC"
                     /locus_tag="LGK42_003144"
     CDS             10127..12325
                     /gene="paoC"
                     /locus_tag="LGK42_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HBK2659368.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLIALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12335..13291
                     /gene="paoD"
                     /locus_tag="LGK42_003145"
     CDS             12335..13291
                     /gene="paoD"
                     /locus_tag="LGK42_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HBK2659369.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHQEEDSCLPPSS"
     gene            13342..13680
                     /locus_tag="LGK42_003146"
                     /pseudo
     CDS             13342..13680
                     /locus_tag="LGK42_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         3494 a         3815 c         3775 g         3070 t
ORIGIN      
        1 attgcggcca gaaagtcaga agccttagca aattattggt attacaatag caatattagg
       61 ggcgtggtat acgctggact gagtcgtgat attagaaaag aactggccta tgtgattaat
      121 ggcaggttcc tgagaaaaga tattaagaaa gataaaatca cggaccggga aatggaaatt
      181 atccgcatga cggcccaggg aatgcaacct aaatcgattg ccagaattga aaattgtagt
      241 gtgaagacag tgtataccca tcggcgtaat gctgaggcca agctgtactc aaaaatatat
      301 aagttggttc agtaaactcc aggcaagtta gttttaaaaa atgactcact gggacatcac
      361 gtcctcaatt caactcggga agaaatgcaa tgaaaaaaaa ggttctggca atagctctgg
      421 taacggtgtt taccggcatg ggtgtggcgc aggctgctga cgtaacagct caggctgtag
      481 cgacctggtc ggcaacagcc aaaaaagaca ccaccagtaa gctggttgtg acgccactcg
      541 gtagcctggc gttccagtat gccgaaggca ttaaaggttt taactcacag aaaggtctat
      601 ttgacgtggc tatcgagggt gactcaacgg ctaccgcctt taaactgacc tcacgtctta
      661 tcaccaacac attaacccag ttggatacct caggttccac actgaatgtg ggcgtggatt
      721 ataacggcgc ggcagtcgaa aaaactggcg ataccgtgat gatcgatacc gccaacggcg
      781 tactgggcgg caaccttagc ccgctggcta acggttacaa tgccagcaat cgtaccaccg
      841 cacaggatgg tttcaccttc accatcatca gcggcaccac caatggtacc accgcagtaa
      901 ccgattacag cactctaccg gaaggcatct ggagcggcga cgttagcgta cagttcgacg
      961 cgacctggac cagttaatct ctctgatgta ccagcagggg tagcccccct gctttattcc
     1021 tggacggaat tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt tccggaatat
     1081 ctccggccca ggcgctggat gtcggcgata tatcatcgtt tatgaacagt gacagcagca
     1141 cgctgagcaa aacgatcaaa aacagtaccg acagtggtcg ccttatcaat atccgtctcg
     1201 aacggctctc ttcaccgctt gacgacgggc aggttatctc aatggacaag ccggatgagt
     1261 tgctactcac tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg atccgcttct
     1321 tctataaggg acccgcagat gaaaaagagc gctactaccg cattgtctgg tttgatcagg
     1381 ccctcagtga tgcgcagcgc gataatgcca accgcagcgc tgtggccact gcttccgccc
     1441 gcatcggcac cattctggtc gtcgcccccc gccaggcgaa ctaccacttt cagtacgcca
     1501 acggctccct gacaaataca ggaaatgcga cgctgcggat cctcgcctac ggcccttgcc
     1561 tgaaagccgc caacggtaag gagtgtaaag agaattacta cctgatgccg ggcaagtcgc
     1621 gtcgttttac ccgcgtggac acggcggata acaaaggacg ggttgcactt tggcagggtg
     1681 ataagttcat tcccgtgaaa tagatagctg tgcagatgga taacgacaat gcctttacga
     1741 cggttctccc caggactgaa agcccagttt gccttcggca tggtcttttt gttcgttcag
     1801 cccgatgcca gcgctgctga cataagtgcg cagcaaatag gtggggtgat tattccgcag
     1861 gccttcagtc aggcgcttca ggacggcatg agcgtcccgc tctatattca tctcgccggt
     1921 agccagggtc gccaggacga tcagcgaatc ggcagcgctt ttatctggct ggatgatgga
     1981 cagctacgca tccggaaaat acagctggaa gagagtgaag ataacgccag tgtcagcgaa
     2041 caaactcgac agcagctgat ggctctggcc aacgccccgt tcaatgaggc ccttaccatc
     2101 cccctgactg acaacgcgca gctggatctc agcttgcgcc aactgctgct gcagctggtg
     2161 gtcaagcgcg aagcgctggg caccgtacta cgctcacgta gcgaagacat cgggcagtcc
     2221 agtgttaaca ccctcagcag taatctgagc tataacttgg gcgtctataa caaccagttg
     2281 cgtaacggcg ggagcaacac atccagctat ctgtcgctga ataacgttac tgcgctgcgc
     2341 gaacaccatg tggtgctcga cggctcgctg tacgggatcg gtagcggtca acaggacagt
     2401 gaattatata aagcgatgta tgaacgcgat tttgccggtc accgatttgc cggtggaatg
     2461 ctcgacacct ggaacttgca gtccttaggg ccgatgaccg ccatttcagc agggaagatt
     2521 tacggccttt cctggggaaa ccaggccagc tccaccatct tcgacagcag ccagtcagcc
     2581 acgccagtga tcgccttttt accggcggcg ggcgaagtac atctcacccg tgatgggcgg
     2641 ctactaagcg ttcagaactt cactatgggc aatcatgaag tggatacccg gggtctaccg
     2701 tacgggattt acgatgtgga agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgc
     2761 ccccagcggg tcaataagct gtttagccgg gggcgcggcg tcggtgcacc actggcgtgg
     2821 caggtatggg gcggtagctt tcatatggat cgctggtcgg aaaacgggaa aaagacgcga
     2881 ccagctaaag agagttggct agcaggtgcc tcgacctccg gctcattgag tacgcttagc
     2941 tgggcggcaa cgggatatgg atacgataat caggcggtgg gtgaaacccg tctgacgctg
     3001 ccgcttgggg gggcgatcaa cgttaacctg caaaatatgc tggccagtga cagctcatgg
     3061 agcagcatcg gcagcatcag cgccactcta ccgggaggct ttagttcgct gtgggttaac
     3121 caggaaaaaa cccgcattgg caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc
     3181 ggcggcacac tcaacctgaa ctcactgtgg tcgaagctgg gcacattcag catcagctac
     3241 aatgatgacc gccgttacaa cagccattat tacacggcag attactatca aaatgtctac
     3301 agcggtacct ttggttcgct tggcctgcgg gccggtattc agcgctataa caacggcgac
     3361 agcaacgcca atacagggaa atatatcgct ctcgatctct cgctaccact gggcaactgg
     3421 tttagcgcag ggatgaccca tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa
     3481 cagtttgatg aaggaaccat tcgcactgtt ggtgccaatc tgtcacgtgc catctccggc
     3541 gataccggtg atgacaaaac cctcagcggt ggggcgtatg cacagttcga cgctcgttac
     3601 gccagcggaa cgctgaacgt caatagcgcg gcggacggct acatcaatac caacttgacc
     3661 gccaatggca gcgtcggctg gcagggtaaa aacatcgctg ccagcgggcg gactgatggc
     3721 aacgctgggg tgatattcaa caccgggctg gaggacgacg gtcagatcag cgccaaaatc
     3781 aacgggcgga ttttcccgct taacggcaag cgtaactatc tcccgctctc tccctatgga
     3841 agatatgagg tggagttaca gaacagcaaa aactcactcg acagctacga tatcgtcagc
     3901 ggccgcaaaa gccatctgac tctctatcca ggcaatgtcg ctgtcattga gccagaggtg
     3961 aagcagatgg ttaccgtctc cggtcgtatc cgtgcggaag acggcacact gctggctaac
     4021 gcacggatta acaaccatat cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg
     4081 gacgtggata agaaatatcc cactatcgat tttcgctaca gtggcaataa aacctgcgaa
     4141 gtggcactgg aactcaacca ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc
     4201 ggcctctcat cgtgggcggc ggtgacgcag acaggagaag agaatgagag ttaacctact
     4261 aataacgatg ataattttcg cgctaatctg gccagtaact gagctcagag cggcagtgag
     4321 caaaacaacc tgggcggatg caccggcacg cgagtttgtg tttgtcgaaa acaactcaga
     4381 cgacaacttt ttcgtcactc ctggcggggc gctggatccg cgcctgaccg gtgccaaccg
     4441 ctggaccggt ttaaaataca ctggttcagg aaccatctat cagcaaagcc tcggctacat
     4501 tgataacggt tacaacaccg gcctttatac caactggaag tttgatatgt ggctggaaaa
     4561 ttcaccagtt tcatctcctt taactggctt gcgctgcatc aactggtacg ctgggtgtaa
     4621 tatgaccacc agtcttatcc tgccgcaaac caccgacgcc agtggatttt atggcgcgac
     4681 ggtgaccagc ggcggcgcga agtggatgca cggcatgttg tcagacgcgt tttaccagta
     4741 tctgcaacaa atgcccgtcg gcagcagctt tacaatgacc atcaatgcct gccagacctc
     4801 tgtgaactat gacgccagca gcggcgcacg ctgtaaggat caggccaccg gcaactggta
     4861 tgttcgcaac gtcacccata cgaaagcagc aaatctgcgg ttaataaata cccactcgct
     4921 ggcggaggta tttatcaaca gcgacggagt accgactctg ggcgaaggga acgccgactg
     4981 ccggacacag accatcggca gcctttcagg attaagttgt aagatggtca actataccct
     5041 gcaaacaaac ggactcagca acacctcaat ccatatattc ccggcgatcg ccaactcgtc
     5101 gttagcctcg gccgtcgggg cgtacgatat gcagttcagt ctgaatggca gttcatggaa
     5161 accggtgagc aataccgcct attactacac cttcaacgag atgaagagcg cagactcgat
     5221 ctatgttttc ttctcgagca acttctttaa gcagatggtg aacctcggga tcagcgatat
     5281 caacaccaaa gatctattca actttcgctt tcagaacacc acatcaccgg agtctggctg
     5341 gtatgaattt tctacctcca acacgctgat tatcaaaccc cgtgatttca gcatcagtat
     5401 tatctccgat gaatatactc agacaccgtc gcgggaggga tatgttggca gcggcgagtc
     5461 ggcactcgat ttcggctata tcgtaaccac cagcggtaaa acagctgccg acgaagtgct
     5521 gatcaaggtg accggacccg cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc
     5581 cgatgacggt aaggcgaaag taccgttccc ggcgacactt tcctttatta cccgcaacgg
     5641 agctacaaaa acctacgatg ccgggtgcga tgatagctgg cgggatatga ccgatgcgct
     5701 gtggttgacc acaccgtgga ctgatatctc tggcgaagtg gggcagatgg ataagaccac
     5761 agtcaaattt tcgattccaa tggataacgc catttcgctg cgtacggtag atgataacgg
     5821 ctggtttggc gaagtcagcg cttcaggaga gattcatgtt caggcgacgt ggcgtaacat
     5881 taactaaagc cctgctgaca gcggtctgta tgctggcggc acctttgaca caggcgattt
     5941 cggtcggcaa tctgacattt tcgctgccgt ccgagactga ctttgtcagc aaacgtgtag
     6001 tgaataacaa caaaagcgca cggatatacc gtattgccat cagtgctatt gatagcccgg
     6061 gcagcagtga attgcgcacc cgaccggtgg atggtgaact gcttttcgcc ccccgccaac
     6121 tggcgttgca ggctggtgag agcgagtatt ttaaatttta ctatcatggt ccacgggata
     6181 accgcgagcg ctactaccgg gtctcatttc gcgaagtccc cactcgtaac cagacaagac
     6241 gtagcccaac cggcggcgag gtcagcacgg agccggtggt ggtgatggat accattctgg
     6301 tagtacgacc acgtcaggtt cagtttaaat ggtccttcga caaggtgaca ggaacggtaa
     6361 gtaacactgg caacacatgg tttaagctac tgattaagcc aggatgtgat tcgaccgaag
     6421 aggaaggcga tgcctggtat ctacgtccgg gagacgtggt tcatcagcct gagttacgtc
     6481 agccggggaa tcattatctg gtctataacg acaaattcat taagattagc gattcctgtc
     6541 cggctaagcc cccttcggcg gactaagact tatccatcgg tcgagggaag aattccacct
     6601 cagagcccca aaaatcgttt aaatgatgtg gaagcgatcg tcaatatcac ctgtaacgat
     6661 gggctttctc atatgcacac catagtgttc aggagacacg gaaaggtatc aggcagcctg
     6721 ctaacgagtg gtttaatcac gaattagtac gtaaaatcgg taacggctgg aaatcattca
     6781 atactcacac tatcgaaagt tccccagcca accgcggtac gttcttacat acgatgtacc
     6841 gctgttctct ttacgatttt tagctgtact ggtgaattat gagcaatctg aatccatgca
     6901 tgacgtgtgg tgcctgttgt gcatttttcc gcgtctcttt ttactgggcc gaagccgacg
     6961 atgctggcgg tactattccc gccaggctca ctgaacaaat atcccctttt caccgatgca
     7021 tgagcggtac caatcagaaa aacccccgat gtattgccct tgcaggaacc ccgggcaaaa
     7081 atgcctgctg cacgatatat aaaaatcgat cgtccacatg cagagaattc gccatgtctg
     7141 gtgagaacgg agtcgtcaat gaggcttgca atcgtgcaag ggctaaatac gggctgacac
     7201 cgttataaac atacaataat taattgcaat gccccgccag cgataatagc ggggcttcgt
     7261 ttttcagggg taacaaaacc cgatacttct tctatttgcc agcaacaatg cctctcttct
     7321 gtagcgttct tcggatctac atcatcctga agtagcgcga tatactcact gactatctgc
     7381 atcagaatat aaaaagcaat gtttttaacc tataaaaatg gcgctgtatt tgcgccattt
     7441 ttatcattca atgcattatc tgtttgagcc taaagggatc tcagggtctg gctcatgagt
     7501 aattctgttt cgtaaatctc tgcgaataat ttcaatagac cagaaccaga ctaaatgtcc
     7561 aaaaatttca gaaacattct cataccacgg gagatcaaac agaggtggcg tcagtcccat
     7621 gagagggaat gaaatcatat gaacaaaaag ttgggctaaa gcacctgcca gtaagccctg
     7681 ccagagttta atttttggaa atacttcagc gaccacacaa taaccgacag caaacactat
     7741 cgaaaagata atgtgcgtaa caccaaccca gttaaagaca tgcccggcaa aggtataaac
     7801 agccgcattg ggatctgtca gccccaacca gtctcgaaga aaaatatacg gtggattgag
     7861 aaaattacgc gagcaatcaa tttggcctgc agccctgatt aatgattccg ggccacacgc
     7921 tgcattaaac atatccaccg ggctacgtgg cggcaatgga acttcagccc cccacttcac
     7981 gaatgcggaa acaacgccag caatcagccc aatgaatgca gcaagaccat aacgtctgcg
     8041 gttcggtgga gtttgttcaa atatattcat atctaccctg cttgtaccat tatgttatac
     8101 acctcttcag gagtattcat aaaacaaggc aaatgtaaag aactgtattg ttttgtataa
     8161 caagatggtt tcctaatcgc caatgaatat aagctccatc atttctccct atttttatat
     8221 tagaagtgac agagatttgc agggtgatgc agagctgaaa atcacaggtt tccttattgg
     8281 tttttgcatc gtacaactaa agcaataaac cagctcccat catcatcaac atcccggcga
     8341 acatgaattt attagccggg ctcatagctg cattcgcgtt ttaagaataa tcctcctgct
     8401 gtcgccgact atgcttaaca tttaaaaaag catcagcact ctcgcaacgc actcttattt
     8461 tcccctttag aataccggag gcctggtatg agcaaccaag gcgaataccc cgaagataat
     8521 cgggttggga agcacgagcc gcacgattta agtttgaccc gtcgcgatct gattaaagtg
     8581 agcgccgcaa cagcggcgac cgccgtggtt tatcctcatt ctacgctggc ggcaagcgtt
     8641 ccggcagcta cacccgcgcc agagataatg cccctgacac tgaaggtgaa cggcaaaacc
     8701 gagcagcttg aggtggatac ccgaaccacg ctactggacg ctttgcgtga aaatctgcat
     8761 ttgatcggta ccaagaaagg ttgcgatcac ggacagtgcg gagcctgtac cgtgctggtc
     8821 aatggtcgca ggcttaatgc ctgcctgacg cttgcagtca tgcatcaggg ggccgagatc
     8881 accaccattg aaggcctggg ttcgccagat aatcttcacc ccatgcaggc ggcctttatc
     8941 aagcatgatg gcttccagtg cggctactgc acctccgggc aaatttgctc atcagtagcg
     9001 gtgctaaaag agattcagga cggcattccc agtcacgtca cggtcgattt ggtttccgct
     9061 ccagaaacaa ctgccgatga gatccgtgaa cgtatgagcg gcaacatctg tcgctgtggt
     9121 gcatacgcta acatccttgc cgccattgaa gatgctgcgg gggagataaa atcatgaagg
     9181 cgtttaccta tgaacgagtg aacaccccag ccgaggcggc acttagcgct cagcgcgtac
     9241 ccggcgcaaa atttatcgcg ggcgggacca atctgctgga cctgatgaag ctggaaattg
     9301 aaacgcccac ccaccttatc gatgtgaacg gcctcgggct cgataagatt gaagtgaccg
     9361 acgcgggtgg gctgcgcatc ggcgcactgg tacggaacac cgacctggcg gctcacgagc
     9421 gcgtgcgtcg tgattacgcg gtactctccc gcgccctgct cgctggcgca tctggtcagt
     9481 tacgtaatca ggcaaccacc gcaggtaatc tgctccagcg cacgcgctgc ccctattttt
     9541 acgacaccaa tcagccctgc aataagcgcc tgcccgggag cggctgcgcg gcgcttgaag
     9601 gctttagccg tcagcacgcg gtggtaggcg taagtgaagc ctgcatagcc acccatccga
     9661 gcgatatggc ggtcgcaatg cggttgctgg atgcggtggt ggaaaccatc acgccggagg
     9721 gaaagactcg cagtatcaca ctggctgatt tttatcaccc accggggaaa acgccgcaca
     9781 ttgaaaccgc cctgcttccc ggtgagctta tcgttgcggt gacgttacct ccaccgctcg
     9841 gcggaaaaca tatctaccgt aaggtgcgcg atcgcgcctc ctacgccttt gccctggtat
     9901 cggtcgcggc gattattcag cctgacggca gcgggcgcgt cgcgctgggc ggagtagcac
     9961 ataagccctg gcgcattgag gctgcggatg ctcagctatc ccagggggcg caggccgtat
    10021 atgacacgct gttcgccagc gcccatccca ccgctgaaaa cacctttaaa ctcctgttgg
    10081 cgaagcgaac gcttgcctcc gtactggctg aagcgagggc acaggcatga aatttgataa
    10141 acccgcaggg gaaaacccga tcgatcagct gaaggttgtc ggtcgtcccc atgaccgcat
    10201 cgacggaccg ctgaaaacta ccggcacggc acgctacgcc tacgaatggc atgaagaggc
    10261 ccccaacgcc gcctatggct atatcgtcgg ttccgccatt gccaaaggac gcctcaccgc
    10321 ccttgatacg gacgccgcgc aaaaagcgcc gggcgtactg gctgtcatta ccgccagtaa
    10381 cgccggggca ctcggcaaag gcgacaaaaa caccgccagg ctgttaggcg gccccactat
    10441 tgagcactat catcaggcca ttgcgctggt agtggccgag accttcgaac aggcgcgagc
    10501 ggcggcctcg ctggtgcagg cacactatcg ccgtaataaa ggagcttact ccctggcgga
    10561 cgaaaaacag gccgtcaatc agccgccgga agacacgccc gacaaaaacg tcggtgactt
    10621 tgacggggct ttcacctccg ctgcggtgaa gattgatgct acctacacga ccccggacca
    10681 gagccatatg gcgatggagc cgcatgcctc gatggccgtc tgggatggaa ataagcttac
    10741 cctctggacc tcaaatcaga tgattgactg gtgccgcacc gatctggcaa aaacgctgaa
    10801 agttcccgtg gagaatgtgc gtattatctc cccgtatatc ggcggagggt ttggcggcaa
    10861 gctgttcctg agaagcgatg cgctgctggc ggccctcgcc gcccgagcgg tgaaacgtcc
    10921 ggttaaagtg atgctccccc gcccctctat tcccaataac accacgcacc gccccgccac
    10981 ccttcagcac ttgcgtatcg gtgccgacca gagcgggaaa atcaccgcta tctcacatga
    11041 aagctggtct ggaaacctgc ccggcggcac gccggaaacg gcggtacagc aaagcgaatt
    11101 actctacgcc ggggcgaatc gtcataccgg cctgcggctc gccacgcttg atttgccgga
    11161 agggaacgcc atgcgtgcgc ccggcgaagc ccccggtctg atagcgctcg aaatcgcgat
    11221 cgacgaactg gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac
    11281 tcaggttgac cccgccgacc cgacgcgctg cttctctcgc cgtcagctta tcgagtgctt
    11341 gcgcaccgga gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtgcg
    11401 cgacggggag tggctagtcg gccacggtgt tgcggcgggc tttcgcaata atctgctgga
    11461 aaaatcgggt gctcgggttc acctcgaaca aaacggcacc gttaccgtag aaacggacat
    11521 gaccgacatt ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg
    11581 cgtaccgctg gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg
    11641 ttctggtgga caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtatgaa
    11701 gcttcgcgaa atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga
    11761 cggcaagatt accaacggta cccgaagcgc cacgctacat gaagccaccg caggcggcag
    11821 actgacagcg gaagagagca ttgaattcgg aacactgagc aaagagtacc agcagtcgac
    11881 ctttgccggg cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg
    11941 gcgtatgctc gctgtgtgtg ctgcaggacg catcctgaat ccgaaaactg cgcgcagcca
    12001 ggtcattggc gcaatgacta tgggcatggg cgcggcactg atggaggagc tggcggtgga
    12061 tgaccgtttg ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc
    12121 ggatatccca aaacaggagg tgattttcct ggatgatacc gaccccatat cctccccgat
    12181 gaaggccaag ggtgtcggtg agctgggcct gtgcggcgtg agcgcggcta tcgccaacgc
    12241 ggtgtataac gccaccggta ttcgggtacg cgattatccc atcactctgg ataagctgct
    12301 cgataagctg ccggatgtgg tttaaggaga aacaatgtca tacccgcttt ttgataaaga
    12361 cgaacactgg cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg
    12421 cttcgccgta gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat
    12481 acgcggcggc gcggcgcgcc cgctcggggc gcagatggtg gtgcgcgaag atggtcgtta
    12541 ctgcggtttt gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat
    12601 gatgggctca ggccgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat
    12661 cgttctgccc tgcggcggtg ggatcacgct gacgctccat aaactacgct cggcacagcc
    12721 tctgctcgcc gtgctgaacc gcctggaaca gagaaaaccg gcggggctgc gctacgatcc
    12781 gcaagcacaa tcgttggtgt gcctgcccac gcaaacccga acgggctgga atctcaatgg
    12841 ctttgaggtg gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc
    12901 gcaggcaacc gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt
    12961 tccggcctca gccagcgctc agatcgatac cgatacggcg gtcattttgc tgtgccatga
    13021 tctcaaccgg gagctgccag tgttgcaggc cgcgcgagaa gcaaaaccct tttatctcgg
    13081 cgcattgggc agccatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg
    13141 gtccagggag gagacagcgc aaatccgggc ccccgtcggg atattcccca aagcccggga
    13201 tgcgcatact ctggcactct ccgtgctggc agaaatcgcc tctgtacgtc tccatcagga
    13261 ggaggattca tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg aaagcgcttt
    13321 ctcgcctccg agggaaatac ccataagtgt atcggctggc gtcagtcccc ggaggttgcg
    13381 ccttatcgct ggccatttga agaaaacggg agaactttcg accttgcgat tgaaccgcag
    13441 attacgacta atgatctgcg tctgatgttg aggctggttc ttgccggcgg aggaataaca
    13501 attgccactc aggaaacttt caggccatat attgaaagcg gtaagcttgt atcgctgctt
    13561 gatgactttc ttccacaatt tccgggcttc tatctgtatt tcccacagcg tcgcaatatt
    13621 gcaccaaagc tccgcaccct gattgactac gtcaaagaat ggcggcagca attggcttaa
    13681 atagctgcac ctgcactgcc tgatgtcaga acagtatttt gatgaattgc caaggttaca
    13741 atggcacaaa tgcggcacag gaggaaacgt ggtgtattta aatatggggt aactctttga
    13801 ttttaatggt gccgataata ggagtcgaac ctacgacctt cgcattacga attataagaa
    13861 tccgcttcta attcaaagca ttaccccatc aacactgcgc tcacacgtcc caccacatca
    13921 aaacatgtaa agccttgcaa gccattgcga ggccttatgt gtctcagttt tgtcccaact
    13981 tgtattacga cttgcatagc caatgaagat aaatgtgacg acaaacggcg cagcagtctt
    14041 cttttccttc atactttccc cacccagcat gcataccttc taccataact gtagtgaatg
    14101 tctgttatga gcgaggagcg gacgttttga atcgaaaaat acaattaatg gcgt
//
LOCUS       DAFAVF010000108        14136 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFAVF010000108 DAFAVF010000000
VERSION     DAFAVF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.108"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            164..1222
                     /gene="citC"
                     /locus_tag="LGK42_003147"
     CDS             164..1222
                     /gene="citC"
                     /locus_tag="LGK42_003147"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBK2659370.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1237..1533
                     /gene="citD"
                     /locus_tag="LGK42_003148"
     CDS             1237..1533
                     /gene="citD"
                     /locus_tag="LGK42_003148"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBK2659371.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1530..2438
                     /gene="citE"
                     /locus_tag="LGK42_003149"
     CDS             1530..2438
                     /gene="citE"
                     /locus_tag="LGK42_003149"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBK2659372.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2449..3981
                     /gene="citF"
                     /locus_tag="LGK42_003150"
     CDS             2449..3981
                     /gene="citF"
                     /locus_tag="LGK42_003150"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBK2659373.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3985..4536
                     /gene="citX"
                     /locus_tag="LGK42_003151"
     CDS             3985..4536
                     /gene="citX"
                     /locus_tag="LGK42_003151"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBK2659374.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4511..5389
                     /gene="citG"
                     /locus_tag="LGK42_003152"
     CDS             4511..5389
                     /gene="citG"
                     /locus_tag="LGK42_003152"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBK2659375.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5440..6903
                     /gene="citT"
                     /locus_tag="LGK42_003153"
     CDS             5440..6903
                     /gene="citT"
                     /locus_tag="LGK42_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBK2659376.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7017..7823
                     /gene="rna"
                     /locus_tag="LGK42_003154"
     CDS             7017..7823
                     /gene="rna"
                     /locus_tag="LGK42_003154"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBK2659377.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="LGK42_003155"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="LGK42_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBK2659378.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8694..9932)
                     /locus_tag="LGK42_003156"
     CDS             complement(8694..9932)
                     /locus_tag="LGK42_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBK2659379.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10153..10581
                     /gene="uspG"
                     /locus_tag="LGK42_003157"
     CDS             10153..10581
                     /gene="uspG"
                     /locus_tag="LGK42_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBK2659380.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="LGK42_003158"
     CDS             complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="LGK42_003158"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK2659381.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="LGK42_003159"
     CDS             complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="LGK42_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBK2659382.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13331..14077
                     /gene="dsbG"
                     /locus_tag="LGK42_003160"
     CDS             13331..14077
                     /gene="dsbG"
                     /locus_tag="LGK42_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBK2659383.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3349 a         3747 c         3559 g         3481 t
ORIGIN      
        1 ttcacgccga aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatca
       61 cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg
      121 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      181 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      241 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      301 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      361 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      421 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      481 ccagtgcggt ttttccacgc tgacctgcgt acccggcgtg atggtgctga tggaaaacag
      541 cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa
      601 gattggctgc attgtgatga acgccaatcc cttcacgaat ggtcaccgtt atctgattca
      661 acaagctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      721 cttcccctat gaagaccggt tggatctggt gttaaaaggc accgccgata ttccacgcct
      781 gactgtgcat cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat
      841 taaagaacag agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca
      901 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg
      961 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
     1021 accgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1081 ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1141 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1201 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1261 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1321 atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1381 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa
     1441 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1501 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1561 ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact
     1621 ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgta gcattgcgtg
     1681 aaaaagacac cgcccgccgc atggtttacc acgcgctgca acatccgctg tatcgcgata
     1741 ttgaaaccat tgtgcgtgtc aacgcgctgg attccgaatg gggtgtcaac gacctggaag
     1801 ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg
     1861 ttctggatat cgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca
     1921 gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1981 ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc
     2041 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc
     2101 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2161 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2221 tgatcaaccc gcgtcagatt gatctgttgc acaacctcta cgcaccaacc cagaaagaag
     2281 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2341 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc
     2401 tctctcgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2461 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2521 cttgccgctt tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2581 aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg
     2641 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2701 atcgcgaaga tgggctttaa aaacctgacc ctggcgtcca gctccctgag tgattgccat
     2761 gcgccgctgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctctggccta
     2821 cgtggtccac tggcggaaga aatctcccgc ggtctgctgg cggaaccggt gcaaatccac
     2881 tctcacggcg gtcgtgtgca tctggtacag agcggcgaac tgaatatcga tgtggctttc
     2941 ctcggcgtcc cgtcctgtga tgaattcggt aatgccaacg gctacaccgg taaagcctgc
     3001 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaaacaggt tgtgatgctt
     3061 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat
     3121 ttaatcgtca aagttgatcg cgttggcgat gctgcaaaaa tcggcgctgg tgctacccgt
     3181 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac
     3241 tcaggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc atcgctggcg
     3301 gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc
     3361 ggtggtatta ccgcgacgat ggttgatctg cacgaaaaag gtctgatccg caaactgctg
     3421 gatgtacaga gctttgacag ccacgctgcg caatcgctgg cccgtaaccc caatcacatc
     3481 gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc
     3541 gacgtggtgg tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3601 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3661 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgactct ggtggataac
     3721 gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggtatc
     3781 gcagttaacc cggcacgtcc ggagctggca gaacgtctgc aggaagcggg cattaaagtg
     3841 gtttccattg agtggctgcg tgaacgtgcg cgtctgctga ccggtgaacc acagccgatt
     3901 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3961 gtacatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     4021 gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgtctgg
     4081 ctcaagcgtc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa
     4141 gacagtgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4201 gcaaaacagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4261 ggtatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4321 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt
     4381 ctctcccgcc gcgactattc actgccgcct cgcagctgcc tgttgtgcga gcaaagcgca
     4441 gccgtctgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggca
     4501 ctgctgaacg atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt
     4561 aatcgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca atctgtcacc
     4621 gaaaccaggc ctcgtggatc ggattaactg cggtgcgcat aaagatatgg cgctggaaga
     4681 tttccaccgc agcgcgctgg cgattcaggg ctggctaccc cgtttcattg aatttggtgc
     4741 ctgtagtgcg gaaatggcac cagaagcggt actccacgga ttacgcccaa ttggtatggc
     4801 ttgcgaaggt gatatgttcc gcgccactgc gggcgtaaac acgcataaag gcagcatttt
     4861 ttctttaggg ctgctatgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac
     4921 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggtctgaccg atcgcgaact
     4981 gcgtaccaat aattcacaac tgacggcagg tcaacggttg taccagcagc ttggccttac
     5041 cggcgcacgc ggtgaagccg aagcgggtta tccactagtg ctcaaccacg ccctgccgca
     5101 ttacctcact ctgctggatc aggggttaga tcctgaactg gcattgctcg ataccttgct
     5161 cctactgatg gcgatcaacg gcgataccaa cgttgcatcg cgcggtggcg aggggggcct
     5221 gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc
     5281 cgatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5341 cggcagtgcc gacctgctga tccttacctg gtttttagca cagatttaat tatttaagca
     5401 cttgataaat ttggaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5461 atatggaaac tattggcccc actggtggtg atgggtgtca tgtttcttat ccctgtcccc
     5521 gacggtatgc cgccgcaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
     5581 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac tatttgcgtt
     5641 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5701 caaaaacagg cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta
     5761 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
     5821 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
     5881 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5941 tttccggtca ttaaaaacct gccgccgctg tttaaatcat tccccaacga tccgtccgcg
     6001 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6061 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6121 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6181 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6241 gaagtggcaa cctgggcggg tgatgaactg aaaaccatgg gtgcgctgac acgcagagag
     6301 tggacactga ttggccttgt attgctcagc ttaggtttgt gggtttttgg cagtgaagtc
     6361 attaatgcta ctgcagttgg tctgctggca gtttcgctaa tgctggccct gcacgttgtg
     6421 ccttggaaag atattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact
     6481 ctggttgtga tggctaacgg cctgactcgt tctggtttta ttgactggtt cgccggtacc
     6541 atgagtacgc acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg
     6601 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6661 gttattctgg ccgttggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg
     6721 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     6781 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6841 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     6901 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg
     6961 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     7021 aagcattctg gcgtaatgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7081 acgccgtagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7141 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7201 gtcgcctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     7261 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7321 gttgcgctac tcgcccaatc ccgaatctac cagaagcgca cgccagccgg atgtgttcat
     7381 cgccggaaac cggattatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     7441 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7501 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg
     7561 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     7621 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7681 gtaaccctgc gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc
     7741 cgctttctgc aaactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg
     7801 tgattgataa agtgggttat taatcatctg atttacaaaa cacgctaatt cgtcttccat
     7861 aaaccgtgac gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8461 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     9001 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactggcgc
     9061 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     9121 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     9181 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
     9241 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     9301 gcaccgagca accgtgcaca ggcgattgtc aacaatccca ctggaccggc accatagact
     9361 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
     9421 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     9481 acattccctt tagggacgcg gacatattcc gcctgcccac caggaacgcc gccatacagg
     9541 tgactataac caaacaatgc cgcgggagct ggtatctgtt ttttattgag cgcagcgcct
     9601 ttacccgcat tggtattttc gcaggcggca tattgctgca ttcgacagaa aaaacagtcg
     9661 ccacaagcaa tgacaaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc
     9721 tttccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
     9781 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgtaata
     9841 cgcagaataa tatcatcagc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
     9901 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
     9961 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
    10021 aattcctggt gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac
    10081 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
    10141 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
    10201 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
    10261 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
    10321 cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac
    10381 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
    10441 gtcaatgagt tggcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
    10501 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg
    10561 ccggtgctgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc
    10621 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
    10681 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
    10741 cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt
    10801 acagtcaccc gctgcgaaca cgcctttcac gttggtttcg catttcgcat caatgataat
    10861 ctcgcccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc
    10921 aatctgcacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatctcg
    10981 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
    11041 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
    11101 ttctggcgca aattccagca gagttacgtg ctcaacgata cccgccaggt cgattgccgc
    11161 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcgggcc
    11221 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc ccggaacgtt
    11281 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
    11341 agaagctgtt tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc
    11401 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
    11461 cagcttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
    11521 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
    11581 aattgctgcc gctgcacccg ccgggccgga accgacgatt aatacgtcat aagcatcacg
    11641 cttgttcagc tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc
    11701 agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc
    11761 catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
    11821 gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttgtg
    11881 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
    11941 cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag
    12001 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg
    12061 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
    12121 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
    12181 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
    12241 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
    12301 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
    12361 gccgcagcac ccgattaatt acgcgattgc ggaacttaga ttttaccaac caggtccaga
    12421 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    12481 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg
    12541 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    12601 cgatcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    12661 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    12721 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
    12781 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    12841 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    12901 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    12961 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa
    13021 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    13081 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    13141 atctgaacta ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca
    13201 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    13261 gctcttacct atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa
    13321 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    13381 agaggaactt ccttccccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac
    13441 gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
    13501 catctacctg actcccgatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
    13561 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
    13621 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
    13681 tgtctacgtg ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
    13741 cccgtgggta gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc
    13801 agaaagcccg gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    13861 agaatatgaa tcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
    13921 acaaatgaaa gtgttaagcg ccaacgagaa actcatggac gatctggggg caaatgtcac
    13981 accagcgatc tattacatga gcaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
    14041 tcagaaaacg cttaatatca ttatggggaa taaataaaag ataattcatg gatggttgaa
    14101 atacatccct gcttttcaac catcctaaaa aagcat
//
LOCUS       DAFAVF010000109        13988 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFAVF010000109 DAFAVF010000000
VERSION     DAFAVF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.109"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            124..690
                     /locus_tag="LGK42_003161"
     CDS             124..690
                     /locus_tag="LGK42_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2659384.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            741..3200
                     /locus_tag="LGK42_003162"
     CDS             741..3200
                     /locus_tag="LGK42_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2659385.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3215..3946
                     /locus_tag="LGK42_003163"
     CDS             3215..3946
                     /locus_tag="LGK42_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2659386.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3943..5004
                     /locus_tag="LGK42_003164"
     CDS             3943..5004
                     /locus_tag="LGK42_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2659387.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5156..6202
                     /locus_tag="LGK42_003165"
     CDS             5156..6202
                     /locus_tag="LGK42_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK2659388.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6199..6990)
                     /gene="nei"
                     /locus_tag="LGK42_003166"
     CDS             complement(6199..6990)
                     /gene="nei"
                     /locus_tag="LGK42_003166"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBK2659389.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7026..7760)
                     /gene="pxpA"
                     /locus_tag="LGK42_003167"
     CDS             complement(7026..7760)
                     /gene="pxpA"
                     /locus_tag="LGK42_003167"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBK2659390.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7750..8682)
                     /gene="pxpC"
                     /locus_tag="LGK42_003168"
     CDS             complement(7750..8682)
                     /gene="pxpC"
                     /locus_tag="LGK42_003168"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBK2659391.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8676..9332)
                     /gene="pxpB"
                     /locus_tag="LGK42_003169"
     CDS             complement(8676..9332)
                     /gene="pxpB"
                     /locus_tag="LGK42_003169"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBK2659392.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9355..10098)
                     /gene="ybgI"
                     /locus_tag="LGK42_003170"
     CDS             complement(9355..10098)
                     /gene="ybgI"
                     /locus_tag="LGK42_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBK2659393.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10369..11850
                     /gene="dtpD"
                     /locus_tag="LGK42_003171"
     CDS             10369..11850
                     /gene="dtpD"
                     /locus_tag="LGK42_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBK2659394.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11892..13310)
                     /gene="phrB"
                     /locus_tag="LGK42_003172"
     CDS             complement(11892..13310)
                     /gene="phrB"
                     /locus_tag="LGK42_003172"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBK2659395.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGDYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13307..13816)
                     /locus_tag="LGK42_003173"
     CDS             complement(13307..13816)
                     /locus_tag="LGK42_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBK2659396.1"
                     /translation="MNQQRFDDSTLIRIFALHELYRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYRQRVIVLLSHPANTRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3390 a         3747 c         3405 g         3446 t
ORIGIN      
        1 attaattact tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc
       61 gacaatcaaa taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactcc
      121 gttatgttta aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca
      181 cctgtttatg cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa
      241 gcaccgtgtg aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc
      301 accagccaca ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gcgcttagtc
      361 aactgtgatc tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc
      421 acctttgata gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg
      481 ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta
      541 ggcaccgcta ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc
      601 tttgcctgga tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg
      661 aatgcaacct acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt
      721 tcataaaata tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct
      781 gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt caaccttaat gttctcgata
      841 aatcaatgcg cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg
      901 gtgagtattt tgttagcgtt gcggtaaata ataaccaaat cagtaacggg caaaagatta
      961 actggcacaa aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat
     1021 ttggcttaaa acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta
     1081 gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa tcagcaacta aatatcacca
     1141 ttccgcaagc ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag
     1201 aaggtgtcgc cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg
     1261 atggcagcag cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat
     1321 ggcgcttacg cagtgattat cagttgaatc agactgatag cgatgataac catgaacagt
     1381 caggcgaaat atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa
     1441 ccctcggcga aacggatttt agttccaata ttttcgacgg tttttcttat accggcgcgg
     1501 cactggcaag tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta
     1561 gcggtattgc acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc
     1621 agaaaaaagt cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca
     1681 cactggatgt caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcggcag
     1741 catcgacgcc cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc
     1801 cacggccctc catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct
     1861 gggggatgct gtcaaacacc tcgctgtacg gcggcctgct gctttctggt gatgactacc
     1921 attctgccgc aataggtatc gggcaaaata tgctgtggct tggcgcgctg tcgtttgatg
     1981 tcacgtgggc cagtagtcac tttgatactc agcaggacga gcggggctta agctaccgtt
     2041 ttaattacag caaacaagtg gatgccacca acagcacgat ttccctcgcc gcttatcgtt
     2101 tctccgatcg tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg
     2161 atgcgcagga cgaaaaacag acgatcagct tatctgtggg ccaaccgatt accccactaa
     2221 acctcaatct ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga
     2281 ccgccaacat cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt
     2341 cgacgtcatt caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt
     2401 cgatttcgct ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc
     2461 acagcaccac acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca
     2521 tgtctgccgg actgcaatcc gaccgtccgg acaatggagc ccaggtgagc ggtaactatc
     2581 agcacctgag ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca
     2641 gttccgtcag cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc
     2701 atcgccgcag ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata
     2761 ttccggttca gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga
     2821 tttccagtta ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa
     2881 cagttgcaga aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac
     2941 tggcttcccg ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc
     3001 ctcccctcgg agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg
     3061 aggaaggaca tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg
     3121 gtgatagcca gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc
     3181 tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct
     3241 tttgttgttg gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat
     3301 tgtgtttaac gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact
     3361 cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct
     3421 ggtcgccctg ccgccgatac aacgtctgga accgaaagca acaacgcagg tacggatagt
     3481 aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat
     3541 gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat
     3601 tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa
     3661 ggtcgaactg cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac
     3721 cgcgtattat ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt
     3781 caaaaccgtg atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg
     3841 cagccagttt tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa
     3901 ctgcagcgga caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga
     3961 gtgttgctcg tcatctgtct gttatttctg ccgttgaagt ctgcgcttgc cctgaactgc
     4021 tactttggtt catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta
     4081 ccaggcaatg ccaaactcgg cgataagatt tgggaatctg acgatattaa aattcccgtc
     4141 tattgtgaca acaataccaa cggcaatttt gaaagtgagc atgtttatgc ctgggtaaac
     4201 ccctacccgg gggtgcagga tcgctattat caactcggcg taacttacaa tggcgttgat
     4261 tatgacgcca accagggaaa aagccgcatc gacactaacc agtgtattga cagtaagaac
     4321 attgacattt atacccctga gcagattatc gcgatgggat ggcagaacaa aatttgctcg
     4381 ggtgatcccg ctaatattca catgtcgcgt acctttctcg ctcgcatgcg gttatacgtc
     4441 aaaatacgag aaatgccgcc gcatgattat caaagtacgc ttagcgacta tatcgtcgtg
     4501 caatttgacg gtgccggtag cgttaacgaa gaccctactg cccaaaacct gaaatatcat
     4561 attactggtc tggaaaacat tcgcgtgctg gattgcagcg tcaatttttc catttcaccg
     4621 gaaacacagg tgattgattt tggtaaattt aatttgctgg atattcgtcg ccacacaatg
     4681 tcgaaaacat tcagtattaa gacgacaaaa tcacaaaatg accaatgcac cgacggattt
     4741 aaagtcagtt cctcctttta taccgaagaa acgttggttg aagaagataa agcattgttg
     4801 attggtaatg gtctgaagct acgtttgctg gatgaaaacg cctcgcctta taccttcaat
     4861 aaatatgctg aatacgccga tttcaccagc gatatgctga tctacgaaaa aacctatacg
     4921 gctgaacttt cgtccattgc aggcaccccc atcgaggctg gccccttcga tacagtggtg
     4981 ctttttaaga ttaactataa ctgatttgcc atgatgccct ttgcagccgg aaagggcatc
     5041 tctttcagaa aattgctgat tttccccttt tccttgattg ctgttatttt ttcctggccc
     5101 ggctttcttt atgctgtatc gatacaccaa agagaaaata atgagggagc gaaggatgcc
     5161 agttttgcag tggggaatgt tatgtgtgct atcacttctc ctttctattg gtttcctcgc
     5221 ggtacatctc ccggcggcgc tattacttgg gccgatgatc gccgggatca tcttcagtat
     5281 gcgcggaatt actctgcaac ttccccgctc cgcttttctc gccgcgcagg ccattcttgg
     5341 ctgcatgatt gcgcaaaacc tcactggttc gattctcacc acgctggccg ccaactggcc
     5401 gatcgtgctt gctattttac tggtgacgct gctttccagc gccatcgtgg gttggttatt
     5461 ggtgcgctat agctcactgc ctggaaatac cggtgcctgg ggttcttctc caggcggcgc
     5521 agcggcaatg gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat
     5581 gcaatatctg cgagtgctgt tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct
     5641 gggtgataac gctgaagcgg ttaaccagca aatcgtctgg ttcccgccag tgagcattaa
     5701 tctcctgctt acgattttgc tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct
     5761 tccttccggc acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct
     5821 catcaccatc gaactaccgg aatggctgct ggcgatggcg tatatggcaa ttggctggcg
     5881 gattggtctt ggtttcgata aacaaatctt actgcgggca ctacgcccgc taccgcaaat
     5941 cctgctgtcg atttttgctc tgctggctat ttgtgcgggt atggcgtggg ggctgacccg
     6001 gtttatgcac attgatttta tgactgccta cctcgccacc agtcccggcg ggcttgatac
     6061 cgtggcggtc atcgccgcag ggagcaatgc cgatatggcg cttattatgg cgatgcaaac
     6121 cctgcgcctg ttcagtattt tgctgacggg gccagccatt gcgcggttta tttcaaccta
     6181 tgcgccgaag cggtcggcct agtgctggca gcctggacac cagtaaaacg ggcgcgagga
     6241 caacgtggtt ttctcaatga tgccgccaca gcgttcgcac agttcgccat cacgatgaaa
     6301 aaccttaaag cgaaacagcg ccccatgatg cttattctca tccacctgcc cccgcgtagc
     6361 gtaagaaaac cgaggaatct ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt
     6421 gagatctttc gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa
     6481 ataattgcca agcccagcca gaaacgcctg atcgagcagt aatcctgcaa actgacggtt
     6541 acgaaagcgc ggtgacaata accgttcctt taccacctcc ggcgtcagat tcggatccag
     6601 cacatcggga ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat
     6661 ctcaatatcc gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg
     6721 caatactcgc gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag
     6781 ctgattatgg ctgtagagcg ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg
     6841 cgtttccaca tgggtaacgt gttgaccgat aagttgtgat tgataagttt ttaactgcgg
     6901 gaaggcaaac cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc
     6961 tgcacggcgg atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaat
     7021 cggggttatg ctgcgacaac aatctccttt tcggcaaatg tagagcgtag tcggcgggcg
     7081 aacgccagtg cgtgctcgcc gtcgccatgc aggcagactg tttgcgccgt gaccgttgcc
     7141 cattcgccgg tgatactttt gactctgccg tgttgcacca tctctagcgt ttgcgccagc
     7201 gcctgttctt cgttttcaat caacgcgcct ggctgacttc gcggcaccag cgagccgtca
     7261 gcctgataac cgcgatcggc aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct
     7321 gcacgaatca gctcgcttcc tgccagccca acgagaatca atgccggatc gcaagcgtat
     7381 accgctctgg cgatggcgtc tgccagttgt gcttctttcg ccgcctggtt gtacaacatg
     7441 ccgtgcggtt tgacatgacg cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg
     7501 ccaatttgat acagcgtctg ggcgtaaacg gtttctggcg gcaactgcat ggcgctgcga
     7561 ccaaaatttt ccctgtcggg aaaactcggg tgagcgccaa ttgcaacacc attttttatt
     7621 gcttcacgca cgcaagcctg catggtttgc gcatcgcctg catgaaatcc acaggcaata
     7681 ttggcagagg aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg
     7741 gcgttcaggt caattttcat tgtgcagccg ccacgctaat tgttcaaaat aacgttgctg
     7801 atcttgccgc gctttcagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc
     7861 gagcggaatt tgcgcaagat ggtacatatc agcctcaatg atgcaggcaa tacgcgggta
     7921 accaccggtg gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gtggcacctg
     7981 caccacgccc ggtaacaaac cgtgagataa cagttcgcga tcggtggtgc gttttaaaat
     8041 ttgcccctgt aagcgatagc ccatgcggtt actttgcgag ctaagctgcc agggcgaacg
     8101 ccagaatgca tcctgcgagg cacgatcgaa ctcatgatat tccggccccg gcaaggcgcg
     8161 aatgcggttg ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcgc
     8221 tttgccaatc gggagtcggt caccatcctt cagtaaacgg ccttccagcc cgccaatccc
     8281 cactttgaga tcggtgctgc atgaccccat taccggcgga acatcaatac cacccgcgac
     8341 cgccagataa ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt
     8401 catcggcaat cgccagccgg tccagacggc attatcatcc agccgcgctt cgcaaccggc
     8461 acccgtcaga gcaaaccacc catcagtttc gaactcaaca gttaactgac cgagcgtgat
     8521 ctccagcgcg ggggcattgg cgtcattacc caccagtagg ttagcaatgc gtaacgcggg
     8581 catatccagt gcgccgcagt ggctgatacc cgactggcga aaaccgtgac gaccgccatc
     8641 ctgcacagtg gtatacatgc ccgcacgaat aatcttcagc atactccctc cttctgcggt
     8701 acaaagcgca cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac
     8761 aggctgagtg aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca
     8821 ccagtctgcg gcccgccgat cccgacagaa cctgccggaa cgagtaagcg cggttcagcg
     8881 cgccgtggcg tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa
     8941 cctaaaaacc agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc
     9001 aacccgcaat gcgccgcgac caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc
     9061 ggaatttcaa taaagcgaga ctcaggttcc agggcctcgc tctcctccca ccagcgttgc
     9121 aaacgctcta tggcatccag cgccagcgac tcaggattac gcaaaatcac cgtaatattg
     9181 ttcatgccgg gaatggcttc aaccacattc ggcatatcca ccagacgctg cgccagtcgc
     9241 cagatccgtt tctggctagc cagcgtcacc ggcggttcca gttccagcac taccgccgtt
     9301 tcacctatca gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc
     9361 aggattagga atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct
     9421 caatgcgcga ataccaccac gttcagtggc atggtgacct gcagcataaa aatgcaatcc
     9481 ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac
     9541 gccaaaacgc gcggcgctat cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg
     9601 ctggaccacc tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc
     9661 aatccaggag gctagttcca gtcccggcac aggcatggtc agctctcccc acggcaccaa
     9721 cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc
     9781 cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac aggttgatat cattcgccag
     9841 caacgttttt aaacggttac gcttcattcc gcgaatgacc ggagactctc ctttccagaa
     9901 gtagccatga tgcacaatga ccgcatccgc gcccagacgc actgcctcat cgagcaaagc
     9961 ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ccacctgcaa
    10021 accattcggc gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc
    10081 cagttcggtg tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag
    10141 cgttacctcg ccagttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg
    10201 ccaaaagacg caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag
    10261 acagcatatt gcataaataa tcagatctgt atcaaagttt taactttaag gaaacacaag
    10321 cacgccgcgc aactattacg tgacgttgcc gtcaattgtg gaattattat gaataaacac
    10381 gcatcacagc cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc
    10441 ttttacggca tgcgtgccct gctgattctc tatctcacca atcaactaaa atacaacgat
    10501 actcacgcct acgagttatt tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc
    10561 ggtggctttt tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgtta
    10621 ttgatggcga tcggtcatgt ggtgttgggt gccagtgaga tccatccgtc attcctctat
    10681 ctgtccctgg cgataatcgt ttgtggctat ggtctgttta aatccaatgt cagttgcctg
    10741 ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat
    10801 gcggcgggta acgtggggtc tattatcgca cctatcgcct gtggttacgc ccaggaagag
    10861 tacagctggg cgatgggctt tggcctggcg gcggttggca tgatcgcggg tctggtcatt
    10921 ttcttatgtg gcaatcgtca tttcactcat acccgcggcg ttaataaaaa agtactgcgt
    10981 gcgacaaact ttctcctgcc taactgggga tggctgctgg ttctgctggt ggcaacgcct
    11041 gcgctgatta ccgtattgtt ctggaaagag tggtcggtat acgccttaat tgtcgcgact
    11101 attattggcc tgggtgtact ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgt
    11161 aaggagctgg ggctgattgt gacgctcacc ttcttcagta tgttgttctg ggccttcgca
    11221 caacagggcg gcagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatgttt
    11281 ggttataccg ttccgactgc tatgttccag tcgattaatg ccttcgcagt tatgctgtgc
    11341 ggtgtgttcc tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc
    11401 tggggaaaat ttgctcttgg tctcggcctg atgagcgccg ggttctgcat tctgacctta
    11461 agcgcccgct ggtccgcaat gtatggtcac tcttctctgc cactgatggt attaggcctg
    11521 gcggtgatgg gctttgcgga actgtttatc gacccggttg ccatgtcgca aattacgcgc
    11581 attgaaattc cgggcgtgac cggcgtatta accggcatct acatgctgct ttctggcgcg
    11641 atcgcgaact atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct
    11701 tccggggcga tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg
    11761 ggcgcactgg cgtgtgtagg actggtactg atgatttggc tgtatcaggc gctgaaattc
    11821 agaaaccgcg cgctggcgct ggagtcataa taggccagca ccatacctga taaaacgctc
    11881 gctgcgtttt atcaggctcc tttcctcgct tcttcgtacg ctgccagcgt tcgcagcctt
    11941 gcctctttgt gatcaactat cggttgcgga taatccagct tcacacctgc tttctgcgcc
    12001 cacttccacg gctcatgcac cgctttccct ggtacatcgc gcagctccgg taaccatcga
    12061 cgaataaact cgccctcacg gtcaaatttc tcgccctgag ttatcggatt gaaaatacga
    12121 aaatacggcg cagcatcagt acccgtagaa gccgcccact gccagccacc gttattagct
    12181 gccaaatcac catcaatcag ctgcgacatg aaatatcgct cgccttcgcg ccagtcgatc
    12241 aacaaatctt tcaccagaaa actggctgta atcatccgta gccggttatg catccagcca
    12301 gtgctgttaa gctgacgcat agcggcatca acaatcgggt atcccgtttt gcctttctgc
    12361 caggcctgta aatgtgcggg attgctttgc cactgcacac gatccgtcca ggcaataaat
    12421 ggacgatgtt tacacaacga ggggtaatac gtcatcagat gacggtagaa ctcgcgccag
    12481 atcagctcac taagccagac actaccggcc ccaccgtcca gcgcctgcgg ctgttcagcc
    12541 aacaagcgat gcaagcactg gcgaggcgat aacccgcccg ttgccaggct ggccgacaaa
    12601 cggctggtgc cttccactgc cggaaaatct cgttgttgct cataatctcc ggcaccgttc
    12661 tggcaaaact ggcgtaattg cgcaatcgcc gctttttctt ccaccggaaa atgcgcagta
    12721 tcgaaagact gacgaggata attcagcgta atggatggcg agggctctat cgatccgcta
    12781 ctacgaactt ttggtgcagc gacgcactcc ggcatccctt cccgcagccg tttcagccag
    12841 gcattcttaa aaggcgtaaa gactttgtac atctcgtgat tgccggtcat caccgcgcca
    12901 ggcggcagga tcacgctgtc atcaaatcct tcacacacca cgttacgcag cgctctttca
    12961 acttccacat cccgcgcccg ctcattcact tcatactgat agttataaaa caggtgggta
    13021 acgctgtttt ccgcgcacac ctgtttaact atttcgacac tggcgacaaa gtcatccact
    13081 tcacggaaca ataaaggaat acctttttcc gcaagcgcta tttgtagccc attcagttga
    13141 gcattgataa gttcagcctg acgcggcgac atgttatgcg tctcccactg gcgtggtgta
    13201 gcgatataca gcgccagcac gcgtgcagac gaattgcggc aggcggcagc aagcgcgaga
    13261 ttatcatgca gacgtaaatc ctggcgaaac cagaccagat gggtagtcat aaaactcctg
    13321 aatggcgcaa gttaatcaca cgcggccaaa gttcgaaata acgctgccct gaaagccagg
    13381 cgtcaggata aagcgccata tagtgtttga tacgcatcag cggcgcaaga agcggttgct
    13441 cgccacggcg ataactgtcg ataagcgcag ccagctgctg gcgttctgtg gaatcaatat
    13501 gtgggcgaaa ataaccctga acgtgcatca aaacgttggt gtgatcgcgc gtgttggcgg
    13561 ggtgagaaag taaaacgatt acgcgttggc ggtactggtt gtaatagtca tccagatttt
    13621 gccaccgatg aatatcagcc acaaacgggc caagtttgcg gtattccggc tgagaatgcg
    13681 ccagaaagac gagtttatag cggctgtgat aatcgagaag cgccccgcgc gttaagccat
    13741 gttctttcag tcgatataac tcatgaagcg cgaagatacg gattagggtg ctgtcatcaa
    13801 atcgttgctg attcattact gctcctgaac ggtttacagg agtaagtgta gacgttgaga
    13861 aatgatattt gtgaagttaa tcctgttgta gcaggacaaa tttacgaaag cctgctcccc
    13921 gcaaggactg acgtcaggta gtttctaacc atagctgccc tttctattca aacgtgaaat
    13981 caataagc
//
LOCUS       DAFAVF010000110        13754 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFAVF010000110 DAFAVF010000000
VERSION     DAFAVF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.110"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..312)
                     /locus_tag="LGK42_003174"
     CDS             complement(<1..312)
                     /locus_tag="LGK42_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBK2659397.1"
                     /translation="MQDIQQETLNECTKSEQSALVVLWEVDLTEVGGERYFFCNEQNE
                     KGESVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VV"
     gene            complement(312..653)
                     /locus_tag="LGK42_003175"
     CDS             complement(312..653)
                     /locus_tag="LGK42_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2659398.1"
                     /translation="MPESEVFNWLPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGMRAFLWRPPKYNRTVRVVCREWSISDNAMYT
                     DFNCTFEEVTH"
     gene            complement(646..3888)
                     /locus_tag="LGK42_003176"
     CDS             complement(646..3888)
                     /locus_tag="LGK42_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2659399.1"
                     /translation="MASLRELIIKISANSQSFQSEIARASRMGQEYYRTMQNGGRQSA
                     AASRDMRRALAEVTEQINTAKSSAMNMAGAFAGAFATGHLISLADEWSSVNARLKQAT
                     QSSDDFQASQRELMAISQRTGTAFADNASLFARSASSMREYGYRSEEVLKVTEAISTG
                     LKLSGASSAEASSVITQFSQALAQGVLRGEEFNSVNESGDRVIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGTLRDEYAAMPDTVSSSATKVENAFMAWVGGANDASG
                     ATKTLTGIMNGVANNIDTVATAAGALVAIGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAALYRAQQAVVAARGTEKQAAAEAKLATAQASLTRNIAART
                     AAQATLNNVTSVSSRLLSGALGLVGGVPGLVMLGAAAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEAADNEEKTRQALEEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLVEATSQLAVEQSRLSGMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQAALD
                     DKQQNALNNTERELTLSRMKGEARERTRLGYVADDLGFVGDAYQTARQTYINNALEAW
                     RNNQANKPKVRGGKSEAEKTEDAYNRLIKQQKEQIALAGQNTELAKMKYQVSQGELST
                     LSEVQKQTLLQNAALIDQKKIREQIAAYESSLADSNASVRASNEAQLLGYGDGTRIRE
                     RLQEMWNIRQEFEQKNNELLRQYQTGEIEEALWKQEKTLNEKYLEERLNDQQDYYAKA
                     DALRSNWNAGLKEGMTNWADSATDYASQAADAVVSTMDGLVSNISEALAGNVVDWRNW
                     GSSILQEVSKILMNAAIVNGLKSLSRTMSGAGGWIGSVGDWLSGAVANAKGGVYTSAN
                     LSAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVSGGGGFV
                     YSPVYHISIQNKGSNGEIDTQSARGLVDLIDSRVVSIMQSSRRDGGLCSA"
     gene            complement(3934..4275)
                     /locus_tag="LGK42_003177"
     CDS             complement(3934..4275)
                     /locus_tag="LGK42_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK2659400.1"
                     /translation="MEVILIALVLGLIPAIIAQSKGRSFWGWWIYGALLFIVALVHSL
                     VIQKDEKTHEQQMVSNGMKKCPYCAELIKEEAIKCKHCGSDLTHNPDSTVSQKTDDEY
                     LEEARRKAGLL"
     gene            complement(4334..4612)
                     /locus_tag="LGK42_003178"
     CDS             complement(4334..4612)
                     /locus_tag="LGK42_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HBK2659401.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRISPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNEAILQWEVVPANNIKEPFTAFENALFAATQ"
     gene            complement(4636..5007)
                     /locus_tag="LGK42_003179"
     CDS             complement(4636..5007)
                     /locus_tag="LGK42_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HBK2659402.1"
                     /translation="MSKNIRNLALATMSGFRHKTVDVPEWEGTTVVLREPSAEAWLRW
                     QEIVKTGDDDTPLSVAERARRNLEADVELFIDVLCDTGMQPVFSADDREQVLAVYGPV
                     HARLLRQSLELITDAEVIKKK"
     gene            complement(5022..5726)
                     /locus_tag="LGK42_003180"
     CDS             complement(5022..5726)
                     /locus_tag="LGK42_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HBK2659403.1"
                     /translation="MSSNFERSQQTKVMISSAPVTAETLSSASFLELSCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSVGTNGVVAATFSLRLKGKPSLTTEPLKLLSDLKSTLQVS
                     ASETLNMSVEASGGVPPYSYVWKKGSSPVSGQTSATFNKASVTSGDAGAYTCEVSDSA
                     SPINKVTSTSCTVTVS"
     gene            complement(5786..6130)
                     /locus_tag="LGK42_003181"
     CDS             complement(5786..6130)
                     /locus_tag="LGK42_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HBK2659404.1"
                     /translation="MREATLYALLSKLAGGQVYPYVVPLTEGKPAVFPPWLVFSVVSD
                     IASDVLDGQAESRITVQIDIWATAPDDADDIREQALDAVRELAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(6127..6576)
                     /locus_tag="LGK42_003182"
     CDS             complement(6127..6576)
                     /locus_tag="LGK42_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK2659405.1"
                     /translation="MTDFSLDFSGLADIARDLELLSRAESNEVLRDASRAGAEVIRGA
                     VIDHAPERTGKLKKNVVILTQRSRRRGEIISGVHIRGRNLRTRNSDNSMKASDPRNAF
                     YWRFVELGTINMPAHPFIRPAFDMTEELAAQVAMKHMNQAIDGVLSK"
     gene            complement(6573..6911)
                     /locus_tag="LGK42_003183"
     CDS             complement(6573..6911)
                     /locus_tag="LGK42_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK2659406.1"
                     /translation="MQAGRLRDRVVILNITTSRTPSGHPEETLKEWATVWAEVKGISG
                     RERISGGAETAQTTVRVWMRFRRDVTAASRLKVLTGAFKGAILNIDGPPIPDARSSRL
                     EILCSQRGGM"
     gene            complement(6921..7226)
                     /locus_tag="LGK42_003184"
     CDS             complement(6921..7226)
                     /locus_tag="LGK42_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK2659407.1"
                     /translation="MMPTLEELRVQCRIDDDNEQENALLMMYLAAAREEAEKFLNRTL
                     HDETVPDQDTTGLVITPLIKLRLMQLVGYWYESREMQDAVPDFFYTGLRMYRFHPGT"
     gene            complement(7238..7426)
                     /locus_tag="LGK42_003185"
     CDS             complement(7238..7426)
                     /locus_tag="LGK42_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659408.1"
                     /translation="MILKQDLKWSPDGMRVEIIRAGEYEDKELPERVREIATAAGIVS
                     DKRTPVARVSDKAKKQHS"
     gene            complement(7476..8681)
                     /locus_tag="LGK42_003186"
     CDS             complement(7476..8681)
                     /locus_tag="LGK42_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK2659409.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATTVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(8696..9346)
                     /locus_tag="LGK42_003187"
     CDS             complement(8696..9346)
                     /locus_tag="LGK42_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK2659410.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMNEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(9324..10565)
                     /locus_tag="LGK42_003188"
     CDS             complement(9324..10565)
                     /locus_tag="LGK42_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2659411.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(10565..10747)
                     /locus_tag="LGK42_003189"
     CDS             complement(10565..10747)
                     /locus_tag="LGK42_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659412.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(10759..12255)
                     /locus_tag="LGK42_003190"
     CDS             complement(10759..12255)
                     /locus_tag="LGK42_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK2659413.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGTRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKTIPGND
                     DVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            complement(12489..12983)
                     /locus_tag="LGK42_003191"
     CDS             complement(12489..12983)
                     /locus_tag="LGK42_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK2659414.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(13108..13458)
                     /locus_tag="LGK42_003192"
     CDS             complement(13108..13458)
                     /locus_tag="LGK42_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2659415.1"
                     /translation="MPQRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRSGVVREAKTVDHIIPKAHGGTDSDSNLQSLC
                     WPCHKAKTARERLK"
BASE COUNT         3112 a         3937 c         3204 g         3501 t
ORIGIN      
        1 gaccaccgtt ccgccgacca gactctgcag gtcttccgcc atcccggtga ccatgccgtg
       61 caggttagag actttcagcg ttggtcttgc actggctcct ttgccgttca tctcaaagcc
      121 gcttccctga atagggtacg cctgatactg tcgcccctgc caggtgaccg actcaccttt
      181 ttcgttctgc tcattacaga aaaaataacg ttctccgccg acctctgtca gatcgacttc
      241 ccagagcacg accagcgcgg attgctccga ttttgtacac tcattcagtg tttcctgctg
      301 tatatcctgc atcagtgagt gacctcttca aaggtacagt taaaatcggt atacatggca
      361 ttatccgaaa tactccactc cctgcagaca acccggacgg tcctgttgta ttttggcgga
      421 cgccacaaaa aagcacgcat cccggcatga cgggataaaa aactgtccag cgcagcgcgg
      481 gaatattcat ctgtgacccg aaataccggt ttaaacgttt tcagatccgc attcagacca
      541 ccagcccgtc gctgttcata tccgtcaccg aactttaccg taataacaga tggctttcgt
      601 gtcgtctcca tcccctcgcg ggggagccag ttaaaaactt cagactcagg cactgcataa
      661 tcctccgtcc cgacgtgatg actgcataat tgacacaacc ctgctgtcga tcagatccac
      721 cagccccctg gctgactgcg tatctatctc gccattgctc cctttattct gaatactgat
      781 gtgatacacg ggagaataga caaatccccc gccaccactc acattcccaa tggctctaac
      841 ccctagggag ccgtcagctg cccgagtcag tggcatgata gcttcaggcc cggcctcgcc
      901 catcagcccg gcacctttcg caaaagcaaa atacgtcggt gtatccacaa tggtgttact
      961 gtaagcactc agatttgccg atgtgtaaac accgcctttt gcgttcgcta ctgcaccgga
     1021 aagccagtcg ccaacactac caatccatcc ccccgctccc gacattgtcc tggagagtga
     1081 tttcagcccg ttaacaatgg ccgcgttcat caggattttt gaaacttcct gaaggatcga
     1141 actcccccag tttctccagt ccacaacgtt tccggccagt gcctcagaaa tatttgaaac
     1201 cagaccatcc atagtggaaa cgaccgcatc tgccgcttgt gaagcataat cagtggcact
     1261 gtctgcccag ttcgtcatcc cctctttgag cccagcattc cagttactgc gtaaagcatc
     1321 ggcctttgca taataatcct gctgatcgtt gagacgctcc tccagatatt tttcattcag
     1381 cgttttttcc tgtttccaca gggcttcttc gatttctccg gtctggtact gtctcagcaa
     1441 ctcattattt ttctgctcaa actcctgtcg gatgttccac atttcctgaa gccgctcacg
     1501 tattcgtgtt ccgtctccat accctaacaa ctgagcctca tttgatgccc tgacactggc
     1561 gttactgtcc gccaggctgc tttcataagc agcaatttgc tcacgaattt ttttctggtc
     1621 gatgagtgct gcattctgta aaagggtttg tttctgcact tctgataagg ttgataattc
     1681 gccctggctg acctggtatt tcatcttagc cagttcagta ttctgtcctg ccagtgctat
     1741 ttgttctttt tgttgtttaa tcagtctgtt ataagcatct tccgtttttt ctgcttccga
     1801 ttttccacca cgtacctttg gcttattcgc ctggttgtta cgccatgctt ccagcgcgtt
     1861 gttgatataa gtttgccttg ccgtctgata ggcatccccc acaaaaccga gatcatccgc
     1921 tacatagccc agacgagtac gctcacgcgc ttcccccttc atacgggata aggtcagctc
     1981 tcgctcagtg ttattcagcg cgttctgctg tttatcatcc agcgcagcct gtggcagccg
     2041 taacggtaca ttcaccagcc cctgtcgctg ctgaagtaat tcattaccaa gcccaagaag
     2101 acgattaaac tcggtatgtt ggccattcat gatcagtaat gactgatatg ctttgttttg
     2161 ttccgctgcc tgttggcgga tcagcaccac tcgtcgttct tccagcccgg caagcacatc
     2221 ctggattgac tgggcttttt cctgcattcc ggaaagtcgg gactgctcaa cagcaagctg
     2281 acttgttgct tctacaagcc cttctgtgac agttttcacc gacgtcatgt ggttaatcat
     2341 aaaaccgtta tcggttgtcc agcccgggtt tgccaagaca tactgatagc cagcaatttt
     2401 ttcctgtaag gatttaatct tacttttctg ctcgtcaatt aacctgttct gctcctccag
     2461 agcctgccgc gtcttttctt cattatctgc cgcttcagga agtgacattg cagacgtttt
     2521 ctggcgaatt tcgtcgattg ttgcggcata ctgacgcgct gattctctgg cctgctcctg
     2581 gttctgatac attgtgtacc aggccgccgc ccccagcatg acaagtccag gcacaccacc
     2641 aaccagcccc agagcaccgc ttaacaaccg actactaact gacgtaacgt tattcagcgt
     2701 tgcctgtgcc gctgttctgg cagcaatatt acgggtaagt gaagcctgtg cggttgccag
     2761 tttcgcttct gctgcagcct gtttttctgt accacgggca gccacaaccg cctgctgcgc
     2821 acgatacaat gccgcccgcg ctctggctgt agctatctgt gtccctctga gctgtgcctc
     2881 agcaagagcc acttcgtttc tggcagcagt gattaacccg gcagttgcag agcccgcaga
     2941 agacgccata ttgccaaaat atcgggctac accaattgca accagagctc ccgccgccgt
     3001 ggcaaccgta tcaatattat tcgcaacacc attcattatt ccggtaagcg ttttcgttgc
     3061 accgctggca tcattcgcac caccaaccca ggccataaag gcgttttcaa ctttggttgc
     3121 agaggatgat acggtatcag gcatggctgc atattcatcc cgcagcgtac caagctggct
     3181 aatcagtgca ggaacaacct tatcggcggt cagctttcca tcatccgcca ttgccttcag
     3241 atctttacgg gcaaccccca ttcctgcagc cagagcacga ataacacgat caccgctttc
     3301 atttactgaa ttaaactctt caccgcgcag aaccccttgc gccagtgcct ggctgaactg
     3361 agtgatcacc gagctggctt ccgcagaact ggctccggac aatttcaatc ctgtagaaat
     3421 ggcctcggta actttcagta cttcctcaga cctgtaacca tattcccgca tggaagaagc
     3481 cgaacgtgca aaaagactgg cgttatcagc aaaagcagta cccgttctct ggctgatcgc
     3541 cattaattcg cgctgtgatg cctggaaatc atcgctggac tgcgtggcct gcttcagacg
     3601 ggcatttact gagctccatt catccgcaag agatataaga tgtccagtgg caaaagcacc
     3661 ggcaaatgcg ccagccatat tcattgccga agacttcgcc gtatttatct gctcggtcac
     3721 ttctgccagt gctcgccgca tatcacggga tgcagctgca gactgtcgac ctccgttctg
     3781 catggtacgg tagtactcct gtcccatacg tgaagctcgc gcaatttctg actggaatga
     3841 ctgcgaattt gccgagattt ttataatcag ttcgcgtagt gacgccattc tcgttctcca
     3901 ggcaaaaaaa aaccgccgaa gcggtttttg cttttaaaga agcccagcct tacgccttgc
     3961 ttcttcaaga tattcatcat ccgttttttg cgaaacggta ctatcagggt tatgtgttaa
     4021 atcactaccg caatgcttac acttaatggc ttcttctttg atcaactccg cacagtaagg
     4081 gcattttttc atgccattgc tcaccatttg ttgctcatgc gttttttcat ctttttggat
     4141 tacaagtgaa tgaaccaggg caacgataaa gagtaaggct ccgtatatcc accatcccca
     4201 aaatgaacgc cctttacttt gcgcgataat agccggaatt aaccccagga ctagcgcaat
     4261 aagaataact tccatcaata tctccttatt tacttaataa aaatcggaat cataatccag
     4321 taaagtaaga agatcattgt gtcgctgcaa ataatgcatt ttcaaacgct gtgaacggtt
     4381 ctttaatatt atttgcagga acgacctccc actgaagaat cgcttcattc agtggaactt
     4441 tgacaccctg cgccccgtaa actgcagaaa ctatctgggc agcccggata tcagcccgct
     4501 cgtcacccag cgggctgatc ctgtcaaatt ctgcccacat catgatttcc gatgcagaca
     4561 tttcccggcg taactctgac aatgtgcgcc ccatcctgag cgccagcatc atcagaaaac
     4621 gaatacccgg aagtgctact tttttttaat aacttcagca tcagtgatca gttccagtga
     4681 ctgacgaagc agtcgcgcat gtaccggacc atatactgca agtacctgct cccgatcatc
     4741 cgctgaaaac acgggttgca tcccggtatc acacagaaca tcgatgaaca gttcaacatc
     4801 cgcttccaga tttcggcggg cacgctccgc aactgataac ggtgtatcat catctccggt
     4861 tttaacgatc tcctgccaac gtaaccaggc ttctgcagag ggttcccgta atacaaccgt
     4921 tgtaccttcc cactcaggaa catcaactgt tttgtggcga aaaccagaca tcgtcgccag
     4981 tgccagattg cggatatttt tagacataga tttcatcctc cttaactgac agttacggtg
     5041 caggaagtgg aggtcacctt attaatcggg cttgctgaat cagaaacctc acaggtatac
     5101 gcaccggcat ctccggatgt cacggaggcc ttgttgaaag ttgccgatgt ctgcccggaa
     5161 actggagagc tgcctttctt ccagacatat gaataaggag gcacaccacc tgatgcctca
     5221 acagacatat tcagcgtttc actggcagaa acctgtagtg tacttttcaa atcagagagg
     5281 agtttcaaag gttcagtggt cagagagggc tttcctttca ggcgcaggga aaatgtggca
     5341 gcaacaacac cgttggttcc cacagaccag gtatgctggc gaacttctgc cataaaggta
     5401 aatccatttc ctgacgggaa aataactttg aaaccgtatg tggtgtcgtt gtcataagca
     5461 ctacgcaacg cgttctgggc agcgttgaga taaaagtttc ctgacatgga aatctctgaa
     5521 gcagccccaa gaccgttgat attctcctgc tcggtagaac acagcgtagt gacatcaata
     5581 tcctgtttct gcccggcggt aaactgaacc tctttaatcg tacagctcag ttcaagaaaa
     5641 ctggcagaac tcagcgtttc tgccgttacc ggtgcagacg agatcatgac tttggtctgc
     5701 tgagaacgtt caaaattaga ggacatactc gtctcctgaa aataaaaaac ccgccagcgg
     5761 cgggtgggta aaatcattaa cgacctcagg caattacctg aaattcaagc gtagctctgc
     5821 tcagacggga atcaggatca taaccctgag ttttagaaat aacggatggt gccagttccc
     5881 ttaccgcatc aagcgcctgc tcacggatat catctgcgtc atcaggagcc gttgcccaga
     5941 tatcaatctg cactgtgatt ctggattcag cctgaccatc aagcacatca gacgcgatgt
     6001 cagacaccac tgaaaacacc agccatggtg gaaataccgc aggctttccc tccgtcagcg
     6061 ggaccacata aggataaacc tgccctccgg ccagtttgga cagaagagca tacagcgtgg
     6121 cttctctcat ttactcagca ccccatcaat agcctgattc atatgcttca tggcaacctg
     6181 tgctgccagc tcctctgtca tatcaaaggc cgggcgaatg aacggatggg caggcatatt
     6241 gatagtcccc agctccacga aacgccagta aaacgcgttt cgcggatcgc tggctttcat
     6301 gctgttatca ctgtttctgg ttcgcagatt ccgcccacga atgtggacac cagagataat
     6361 ttcaccacga cgccgtgaac gctgagtcag aataaccacg tttttcttca gttttccggt
     6421 tcgctctggc gcatggtcaa taaccgcccc ccggataacc tctgcaccgg cacgggaggc
     6481 atcgcgaagc acctcattac tctctgctct gctgagaagt tccagatctc ttgcaatatc
     6541 cgccaggcca gaaaaatcca gactaaaatc cgtcacattc cacctctctg gctgcaaagt
     6601 atttcaagcc tggaggatcg tgcgtccggg atcggcggcc cgtcgatatt cagaatggct
     6661 cccttgaatg ccccggtcag cactttcaga cgtgaggcgg ccgtcacatc gcgccgaaat
     6721 cgcatccaga ctcttactgt tgtctgcgcg gtttccgcac cgcccgatat acgttcccgc
     6781 ccactgatcc ccttaacctc tgcccatacc gtagcccact ctttcagggt ttcctccggg
     6841 tgaccggaag gggtacggga ggtcgtaata ttcagaataa ccacgcgatc acgtaatctt
     6901 cctgcctgca tgaaatcctc ctacgttccg ggatgaaacc gatacattcg gagtccggta
     6961 tagaaaaaat caggtacagc atcctgcatt tccctgctct cgtaccagta gccaaccagt
     7021 tgcataagac gcaactttat cagcggtgtt attacaagcc cggtcgtatc ctgatcagga
     7081 acagtttcat cgtgaagtgt ccggtttaaa aacttttcag cctcttctct ggctgctgcc
     7141 agatacatca taagaagtgc attctcctgt tcattgtcat catcaatccg gcactgaaca
     7201 cgaagctctt ccagagtagg catcatttgg gctacctcta tgaatgctgt tttttagcct
     7261 tatcagatac ccgtgcaaca ggtgttctct tatcagaaac aatccctgct gcagtggcaa
     7321 tttcacgtac ccgttcgggt aattctttat cttcatactc accagcacga atgatctcaa
     7381 cgcgcatacc gtccggtgac catttcagat cttgtttcag gatcatgatt ctttcacctg
     7441 tcagaacagg ggcgcaattc agcgcccctg agtgattacg ccgctgcaat cttcagcagt
     7501 ttgatggcct gcgaatcgac cagcatgccg ccggtgcgct tggtggtata aaaaccgaca
     7561 aacggtttat tggtgtacgg atcacgcaga atgcgggtac cgatacggtc aacgatggtg
     7621 taaccccgtt tgaagttacc aaatgcaata gctttcgcat cagcggcgat atccggcatc
     7681 tgttcgtttt cagcgatacc gtaacccgcc agagaggacg gttgccccag ctccagcccc
     7741 ggacgccaca gatagttacc ctcggtgtct ttcagcagac ggatggcaaa caggctgttg
     7801 ttgttcatca tgaacttcgc gccagtgcgg tgtgccttac gcagcgtgta aatcagtttg
     7861 ataatggcgt ctgcggtcac cgtcgtcgct tcaccggata caatatgctg aagtttaccg
     7921 aacgcacgaa ccttatcggt ttcatcggtg gattcatacg ccaggaaccc ttttggcttc
     7981 ttggtgccat caccggtggt aaaggcaatt tcttcctgtt cggcaaattc ggtcgccagc
     8041 tcgctgttga tccaggcctc cacgttgaag aaggcatcat ccagcatttt ctgagtggcc
     8101 tgcgggttac cgtagatttc ccccatgaaa ggttcaatca gtcccagttt tgaggtggca
     8161 gtctgggagc gcgcgtcagt ctcgcccacc catccggaag ccgtgccgcc cagattcacc
     8221 agttttttgt agtcggaacc gccaacagtg atcaccgtgg cttcctggcg catcaccact
     8281 tcatctttca gcagggtgag aatgttgcga tccagtgctt ccggcacggc atagccaccg
     8341 tcttcatcgg tgcccacctg caatgccttg cgctccagat cgcgcagacc gtcttcacgg
     8401 cctttacgca gaaagcccac aaacgcctct ttgtgctctg tggccagttt attttgcgca
     8461 ccacctgccg gacgtttcag ctcaagcagc tctttttcaa ggtcgctttt gagattttcc
     8521 agctcgctga gtttcccgtt cagggtttcc acctgcccgg caagtttgcc tttttcctgc
     8581 tcaatcgcat ccacgcgctt gtcgttcttt gctttgaagt cgtcaaactt ctgctgcagc
     8641 tcctgcgcga cctgttcgac atctttaata tcaaccgcca tcgtatttct cctgattaga
     8701 agttcagatt tttcagtgca ttcagtgcag agcccacatc ctcagcgtcg cgcagggaca
     8761 gtgcgccata gcccccggcc atgaatgctt tggcctgggt acgggagagt ccgacatcac
     8821 gcaggactct ttcgattttt ttctgttcgg ggatttcccc gcgggccagt gcgttcttga
     8881 cgtcgctgat ccgcgcctcg tcgttagacg ggaacgtcac caggctgact tcccagaggt
     8941 cgatttcttt cagcagaaag gcttctttgc tccggtcgta ttcccagtct ttcaggacgt
     9001 acccaataga aaggccggtt aacgaaccgg ccttcatgtg tgcatgtgcg cgttttgcga
     9061 ggggatcatc atcaatgagc aaccgccccc tgacgtaaag cccgacatcg tcttcgttca
     9121 tttcggtgta aacaccgatg ggctcatcca tgcggtgctg ccagagcagc gcaggtaacg
     9181 cttttctgtc actccacgcc cgcagggaag cagcaaatgc cccggacatc accacatcat
     9241 cgtggctgtc ctttacacca aagacggagc catacccttc aaactcaccg gagtcactga
     9301 cagatttcag actcagcggt acatcaagac gttgtttcgt ctgcattggc gttatccttc
     9361 tgcttaccgg ctttactacc atcggagggt ttcgtggtca tgttcatcgg tgtgagatag
     9421 acatcaccac cgggacgcgg attcatatct tccaggtcgc ggcagtcatt gggagagtaa
     9481 attccccagt tgatcccggt ggcgtaggct tcaaaacggg acttcatatc cccgcgcagt
     9541 aacgccccgg cgttaaattt ggcgtaataa acgccctgct tactttttcg taccagtccg
     9601 gtgttgatcc gctgctcaat acgggtcaga tacggcacca gtgaatagtt gataaatccc
     9661 agccccagct cttcgatatt gttgaaggtg gcgcgatcgg tgttctgcac catgtgcaag
     9721 ggcacacgga acagacgaca gatttcttca agctgaaact tgcgggtttc caggaactgg
     9781 ctgtcctcgg cgttcaatgc catcgacttc cagtccagcc ccatctcaag gatcatcggg
     9841 cggtgagcat tgccaagccc ggtgtgacgc tcctcaaaat ctttcttcag gcgctcataa
     9901 gcctgatccg acagcgtctg ttctgtacgc aacacaccgg acgtcaccgc gccattgctg
     9961 aacagtctgg caccgtgctc ttcagtcgct gctgccagcg atattgcctc gcgggcatag
    10021 gcgatgggat tcagtcccac cagaccgtcc agcgtcagcg tgcgcacatg ccagatatcc
    10081 tcctgggtca gcacatccgt tgagccgtcc gggaatgtga cctgatagac cggctcccag
    10141 ctactgttaa gcttcggtac cacacagccg ggatcgacgg gcagcagttc agccacttcg
    10201 ccaaatgctt tcactttgta ggcgtaaaag tttccccgca ggcacagaca ggtgaccacc
    10261 agctcccaga actcctgcgg cgtcatatag ccattgggat gcgtggagat cagcttatgc
    10321 agacgttcgc cagtggctct ctgcttcagg ctgccgttca ggtgatacag attgcagggc
    10381 aacatcccga ccgactctgc cagcaccctg acgcaggaaa aaaccgccgt cagtcgcatg
    10441 gcccgctggc tgctgatctg ctttccggta taggtgtcat atgacagccc gatagcatcc
    10501 gccagctctg ctggcgtggt caccggcgcg tcactttttc gttgaaataa tcccgaaaag
    10561 aacactattt acctccgccg acagacgact gtgtacggtc gagatatcgc gccaccagcc
    10621 acgaccagaa caggcacaac gccccggcaa caacaaaccc cgccggggga taaatcagcc
    10681 aggcaccata cgccagcaaa agcgccccca gcacgcccac cagaggcgcg agaatcagca
    10741 tgatcataat tacctcagtt aaagcgagcg gatcccatag gactcaatgt ggtcagacag
    10801 cgtgtcttct ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc
    10861 atcgattttg ttttccgcct gctctttgac gggcttcacc acatcatcgt tacccggaat
    10921 ggttttgccg accacgttgc cgatacacca ggtcatgatg ggattgccat catgatgaaa
    10981 gcgccccgat tcaattgccg cttccagctc tttcatcggg tcggacatgt tggtgtagtt
    11041 ctgaatgata gtgatggggt tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc
    11101 cccgaagggg tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc
    11161 ctcgaggatg tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc
    11221 cacccatttc tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac
    11281 cgtgtcatac ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat
    11341 ctcgcgggta taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc
    11401 cagaatgcac ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg
    11461 ccagctcacc aggttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt
    11521 tttaaagacg tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa
    11581 ttcgcgataa accgacacgc caatatttgg attggctttt tccagcacct gcgggtcggt
    11641 ccagtcgtca ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga
    11701 gccattgagc atctcgataa cttcccgccg cttgtcgtag cacggcccct caatgttgta
    11761 cccggcggtg gtgatagccc acatcagtgg ctggcgtcgc gcccccatcc cggtaagcat
    11821 tgtggtgtaa agcgcatctg tggcgtgctc gtgatattca tccaccacgg cacagtgggg
    11881 tgatgaacca tcaccggggt taccgatcag cggttcaaac cgcgcaccat cctccggacg
    11941 gttcatgttt gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt
    12001 acacatcagt cgtgccggac gaaagacttc ccatgcctgt ttctccgtcg tggcaccgga
    12061 atacacttcc gcgccgaact cgttatcaca ggcaaaacaa tacagggcga caccggcaga
    12121 gattgccgat ttgccgttct tacgggggat ttcggtatac acctcacgga agcggcgcag
    12181 ccgggtacct ttattgaccc agccaaacgc acagcagatc acaaagagct gccacggctc
    12241 cagcgtgatg ggcatccgct tgaatgccca ttcccccttg gtatgcggca acagctgaat
    12301 aaatttggcg gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt
    12361 ttccgccatc aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac
    12421 aatctttccg cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga
    12481 tttccggctc atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc
    12541 aggccaatca gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctactc
    12601 atctcggact cctgttcttt tttggctgtc agctccggat ttttgaccct gccgcccatt
    12661 gcgccggtga tggtgttgcc ctgtatggca atatttttca cagcacgtcg ccagaactca
    12721 taggccacgc accaccgctc aagcaccgcg aggtcagtca cgcacagcag gccctgaccg
    12781 cagagttctt tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg
    12841 aaccactccg gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg
    12901 gctcgcttgc cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg
    12961 gaacgccccg ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt
    13021 ataaaaaaac gatggggcgg gcagtcctga aggcgtcagg tcacagggat ttgacccgcc
    13081 ctcccctctg gcagtgggaa ctggttctta cttcagccgt tcacgggccg tcttcgcctt
    13141 atgacacggc cagcacaggc tctgcagatt gctgtctgaa tctgtgccgc catgcgcttt
    13201 aggaatgatg tggtcaacgg ttttcgcctc acgcaccaca ccagaacgca gacataactg
    13261 acacaggcct ttgtcacgct tcaggacacg cgcgcggata ctgtcccact tcgaaccgta
    13321 gccgcgctga tgacgggatt gtccaggttt gtattgcttc cagccttcgc ttttgtggct
    13381 ttcgcaatag cctgacgggt cagtggtggt atggcggcag ccgcgaacac ggcaggcttt
    13441 tggggttcgt tgcggaatgt tattcctcct gtgtaattac tgattccatt aatgtaatgt
    13501 aatatattgt attgtaagaa aaaattgttt ttttacagtg atatattttt atagaagact
    13561 attcgctcaa ggtgcattaa gattaatctc acccccacga agaacgttaa ggataagaag
    13621 atgataaaaa tgattaactg tactgcttcc aattgtggcg ttggatacgt ttttggcaaa
    13681 ggcgttgatg ctgagctaat caatagtcac gcaagagcat gtaaaatcgg ttttgtgcaa
    13741 gcagggaatg aaaa
//
LOCUS       DAFAVF010000111        13726 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFAVF010000111 DAFAVF010000000
VERSION     DAFAVF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.111"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            289..1341
                     /locus_tag="LGK42_003193"
     CDS             289..1341
                     /locus_tag="LGK42_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBK2659416.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1657..2859)
                     /locus_tag="LGK42_003194"
     CDS             complement(1657..2859)
                     /locus_tag="LGK42_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK2659417.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3091..4590
                     /gene="pitA"
                     /locus_tag="LGK42_003195"
     CDS             3091..4590
                     /gene="pitA"
                     /locus_tag="LGK42_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBK2659418.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4661..4996)
                     /gene="uspB"
                     /locus_tag="LGK42_003196"
     CDS             complement(4661..4996)
                     /gene="uspB"
                     /locus_tag="LGK42_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBK2659419.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5387..5821
                     /gene="uspA"
                     /locus_tag="LGK42_003197"
     CDS             5387..5821
                     /gene="uspA"
                     /locus_tag="LGK42_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBK2659420.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            6139..7608
                     /gene="dtpB"
                     /locus_tag="LGK42_003198"
     CDS             6139..7608
                     /gene="dtpB"
                     /locus_tag="LGK42_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBK2659421.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7657..8409)
                     /gene="rsmJ"
                     /locus_tag="LGK42_003199"
     CDS             complement(7657..8409)
                     /gene="rsmJ"
                     /locus_tag="LGK42_003199"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBK2659422.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8417..10459)
                     /gene="prlC"
                     /locus_tag="LGK42_003200"
     CDS             complement(8417..10459)
                     /gene="prlC"
                     /locus_tag="LGK42_003200"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBK2659423.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10662..11504
                     /gene="rlmJ"
                     /locus_tag="LGK42_003201"
     CDS             10662..11504
                     /gene="rlmJ"
                     /locus_tag="LGK42_003201"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBK2659424.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11576..12928
                     /gene="gorA"
                     /locus_tag="LGK42_003202"
     CDS             11576..12928
                     /gene="gorA"
                     /locus_tag="LGK42_003202"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK2659425.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12982..13065)
                     /locus_tag="LGK42_003203"
     CDS             complement(12982..13065)
                     /locus_tag="LGK42_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBK2659426.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
BASE COUNT         3187 a         3588 c         3458 g         3493 t
ORIGIN      
        1 gttcgatgat tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc
       61 atccctggaa gataattgta aaaaaaacaa cagaataaga attaatctta ggataaatat
      121 ttatttatca tggcctttta accggtaaaa aatggcactc ggcacagtaa accccgctat
      181 agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta
      241 tcattaatgc acatttcatt attagcaggg atacctgagg agtttaaagt gaacatatat
      301 atcgggtggc ttttcaaatt aatccctttg attatgggat taatttgcat cgcgttaggt
      361 ggctttgtgc tggaaagttc agggcaaagc gagtatttcg tcgcgggtca tgtgctgatt
      421 tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcagctc
      481 acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca
      541 attatcacca tgatatgggg ttgggcactg ttagcaggca atgatgtgat ggcagacgag
      601 tttgtcgccg gccatgttat tttcggcgtt ggtatgattg ccgcctgtgt atcgacggtg
      661 gcagcgtcat ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgat
      721 ggaacgccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt
      781 ttactcaccc tgctcggttt catttggtct attacgctgt tacgtagtgc tgacataact
      841 ccgcattatg tcgcgggtca cgtattgctt gggttaaccg caatctgtgc ctgtctaatt
      901 ggccttgttg ccacaattgt ccatcaaaca cgtaatacgt tttcaactaa agaacactgg
      961 ctgtggtgtt attgggttat ttttctcggc tcaatcacgg tactgcaggg gatatacgtc
     1021 ttagtcagtt ccgatgcaag cgcccgactg gctcccggca ttattcttat ttgcctcgga
     1081 atgatctgtt acagcatatt ctcaaaagtc tggctactgg cactggtatg gagacgtacc
     1141 tgttcgttag ccaacagaat accgatgatt cccgtcttca cctgcctgtt ttgccttttc
     1201 ctggcatcgt ttcttgcgga aatggcgcag accgacatgg gatattttat tccttcgcgg
     1261 gttttggtcg gtttaggagc ggtatgcttt acgttgttct caatcgtttc aatattagaa
     1321 gcaggttctg ctaaaaaata attgcaacgt accggataaa accagcgttg accatttgcg
     1381 taacgctggt ttttcttagg catcatgaaa taacgcagca ttaatgcata gtggttaagg
     1441 ataaaaaaac aacaaagtta ctgttttttt caacctgttc atattttata aagatctggc
     1501 cactgggtga gttttcaatc acctttcctt ccaccttata ttaagcctgg agggtttcag
     1561 ttcacgggct cattagaaaa taatcacaag atagccacta tcaaaattag ctcattttta
     1621 atgcggctct aataattttc atctttaggt aacaggtcag gacgacttcg ctgcaatcaa
     1681 atcctgcgca caagcccacg cactcgacca cgcccactgg aagttatagc cccccagcca
     1741 gccggtaacg tccatcactt cgccgatgaa gtacagccca ggcactttgc gcgcttccat
     1801 cgtccgtgaa gagagttcgt tggtgtccac gccgccgagc gtcacttcgg cagtgcgata
     1861 gccttcagtg ccgttgggtt gtacgcgcca gtcggtcaat gtgctaatca gtgcctgttg
     1921 gtcacgcacg ttgagctgtt ttagcgaaac atccgggatt tgcccgagtt gctgtaagcg
     1981 ttcaaccaac cgcttcggta gatgaaccgc cagtgtgttt ttcaggctct gattcggatg
     2041 tgcgttacgc tgctcattca ggaaggtttc gagatccaca tccggtagca gattgatact
     2101 gacaaattca cccggttgcc agtagctgga aatctgcaac accgccggtc cagacaagcc
     2161 gcgatgggtg aagagtaagt tctcacggaa aacgatgccg ttttcagcgg taatcacgga
     2221 aggcaccgcc acgcccgcca gcacctgtaa ctcttcgagc aacggtttat gcagagtgaa
     2281 tggcaccaga cccgcgcggg tcggcagcac gttgaggcca aattgttcgg caatcttata
     2341 accaaacggc gacgcgccca gccccggcat tgacagccca ccggtcgcga tgaccagctt
     2401 ttcgcaaccg acagtcatgc cgttcagttc aagcgtgaag cctgtttcat ccttcgccac
     2461 actcagcact tcgctacgca atctgaaggt tacattgccc ttctcgcact catccaccag
     2521 catgtcgaca atctgctgcg cggagtcatc gcagaagagt tgtcctaacg ttttctcgtg
     2581 ccaggcgatg ccgtgtttat tgaccagatc aatgaaatcc cactgggtaa aacgcgcgag
     2641 tgcagactta caaaaatgcg gattctggct cagataagcg cctggttcga cataaaggtt
     2701 ggtaaagttg cagcgcccac cgccagacat aaggattttg cgccctggtt ttttaccatt
     2761 atcgatcagc agaacccggc gtcctgcctg acctgccagc gcagaacaga acatacccgc
     2821 cgcaccagcg cctataataa tggcatcaaa cctttccacg ttgcgctcct cttagaaaaa
     2881 acgggcgtga attgtaaaga ttcctcagtg gtcgcaccag catcaatatt actaaaagga
     2941 agtatttgcc tgaattatat aagataatta ttttttgagt gaaatccata caggaggcaa
     3001 atcaaaaaaa gtctatattt cactttgccc gcgccgcgaa agtcactgat aatgcgccgc
     3061 gttcatgtcc tcaaaatggc gtaacgtcct atgctacatt tgtttgctgg cctggatttg
     3121 cataccgggc tgttattatt gcttgcactg gcttttgtgc tgttctacga agccatcaat
     3181 ggtttccatg acacagccaa cgccgtggca accgttatct atacccgcgc gatgcgttct
     3241 cagctcgccg tggttatggc ggcggtgttc aactttttgg gtgttttgct gggtggtctg
     3301 agtgttgcct atgccattgt gcatatgctg ccgacggatc tgctgcttaa tatgggatcg
     3361 tctcatggcc ttgccatggt gttctctatg ttgctggcgg cgattatctg gaacctgggt
     3421 acctggtact ttggtttacc tgcatccagc tctcatacgc tgattggcgc gatcatcggg
     3481 attggtttaa ccaatgcgtt gatgaccggg acgtcagtgg tggatgcact caatatcccg
     3541 aaagtattaa gtattttcgg ttctctgatc gtttccccta ttgtcggcct ggtgtttgct
     3601 ggcggtctga ttttcttgct gcgtcgctac tggagcggca ccaagaaacg cgcccgtatc
     3661 cacctgaccc cagcggagcg tgaaaagaaa gacggcaaga aaaagccgcc gttctggacg
     3721 cgtatcgcgc tgatcctttc cgctatcggc gtggcgtttt cgcacggcgc gaacgatggt
     3781 cagaaaggca ttggtctggt tatgttggta ttgattggcg tcgcgccagc aggcttcgtg
     3841 gtgaacatga atgccactgg ctacgaaatc acccgtaccc gtgatgccat caacaacgtc
     3901 gaagcttact ttgagcagca tcctgcgctg ctcaaacagg ctaccggtgc tgatcagtta
     3961 gtaccggctc cggaagctgg cgcaacgcaa cctgcggagt tccactgcca tccgtcgaat
     4021 accattaacg cgctcaaccg cctgaaaggt atgttgacca ccgatgtgga aagctacgac
     4081 aagctgtcgc ttgatcaacg tagccagatg cgccgcatta tgctgtgcgt ttctgacact
     4141 atcgacaaag tggtgaagat gcctggcgtg agtgctgacg atcagcgcct gttgaagaaa
     4201 ctgaagtctg acatgcttag caccatcgag tatgcaccgg tgtggatcat catggcggtc
     4261 gcgctggcgt taggtatcgg tacgatgatt ggctggcgcc gtgtggcaac gactatcggt
     4321 gagaaaatcg gtaagaaagg catgacctac gctcagggga tgtctgccca gatgacggcg
     4381 gcagtgtcta tcggcctggc gagttatacc gggatgccgg tttccactac tcacgtactc
     4441 tcctcttctg tcgcggggac gatggtggta gatggtggcg gcttacagcg taaaaccgtg
     4501 accagcattc tgatggcctg ggtgtttacc cttccggctg cggtactgct ttccggcggg
     4561 ctgtactggc tctccttgca gttcctgtaa tcgtacacac caaaacgagc gggtcagttt
     4621 actggcccgc tttttttatg cgttattatt gctgaagtgc ttaatgccaa atcatcaatg
     4681 caatcaggct gaccaccacc agaccacaca atgcgctggt cagaataaac tgccgacgca
     4741 cccgctcaca gcggcgaata aattcatcat cgtgatgatc gcgataacgt tgggcataga
     4801 tataccaaac gagacgcacc tgtttgttgg gttggccatg tgaggtaaaa aagccccctc
     4861 catcaacata ttgatagagc aatggatcgc agttacgcag taccactaac aacgcgcgta
     4921 gtgatgagaa atagcgcgcc atgttaacaa tgcaaacgac acataaagcc caaaataatg
     4981 cgacggtgct tatcatacct cctccccggc gacctgcccg cggagttcca ccccggggct
     5041 accgctcccg atacgctgcc aatcagttaa caccaggtcc tggagaaacc gcttttgtgg
     5101 tgaccaacat ccgagcggct ctatagatag tgtaggagat caggttgttt tttttccaga
     5161 aggttaacca ctatcaatat attcatgtcg aaaatttgtt tatctaacgg gtaagcaagg
     5221 cggattgacg gatcatccgg gtcgctataa ggtaaggatg gtcttaacac tgaatcttta
     5281 cggctgggtt agccccgcgc acgtagttcg caggacgcgg gtgacgtaac ggcacaagaa
     5341 acgctagctg gccagtcatc gacaacttta tggaaggagt aacactatgg cttataaaca
     5401 cattctcatc gcagtcgatc tctccccgga aagcaaagtt ctggtagaga aagcagtctc
     5461 tatggctcgc ccctacaatg cgaaagtttc tctgatccac gtagatgtaa actactctga
     5521 cctatacacc gggcttattg atgtgaatct gggtgatatg cagaaacgca tctctgaaga
     5581 gacacatcat gcgctgaccg agctttccac taatgcaggc tacccaatca ctgaaaccct
     5641 gagcggcagc ggcgacctgg gccaggttct ggtcgatgca atcaagaaat acgatatgga
     5701 tctggtggtt tgtggtcacc accaggactt ctggagcaaa ctgatgtctt ccgcacgtca
     5761 gctgatcaac accgttcacg ttgatatgct gattgttccg ctgcgcgacg aagaagaata
     5821 atcatccctc cacgacgtgt tcctgaacgc ccgcatatgc gggcgttttg ctttttggcg
     5881 cgccttgtta cctgatgcag cgtaaacccc ttatctggcc tacgatctgc gtacgcaatc
     5941 aaaatctcct gccaatacaa catttaacac catcatattt tccatcatta gtgtgatcag
     6001 ctggttattt tctgttgtaa tagtgtatta atctattcac cgcatcaata ttaagaatct
     6061 ctgacagatg taaacttttt cgcgcgttat cccttacgcg ttcatacttt tcaggatggt
     6121 attggaaggt taataaatat gaatacaaca acacccatgg ggatgctgca gcaacctcgc
     6181 ccatttttca tgatcttttt tgtcgagtta tgggagcgat tcggctacta cggcgtgcag
     6241 ggcgtactgg cggttttctt cgttaaacag cttggattct cgcaagagca ggcttttgtc
     6301 acttttggtg cttttgctgc gctggtctat ggcctcattt ccattggcgg ctatgtcggc
     6361 gaccacctgc tggggaccaa acgcaccatt gttctcggag cacttgtgct ggcgattggc
     6421 tacttcatga ccggcatgtc gctacttaag cctgacctga ttttcatcgc cctggggact
     6481 atcgctgtcg gtaacggcct gtttaaagct aacccagcca gcttgctttc gaagtgctat
     6541 ccgccgaaag atccgcggct tgatggcgca ttcaccctgt tctatatgtc gatcaatatc
     6601 ggctcgttga tagcgttatc gctggcccct gtgatcgctg atagattcgg ttattcagtc
     6661 acctacaacc tgtgcggggc ggggttaatt atcgcattac tggtttacat cgcctgtcgt
     6721 ggaatggtga aagacattgg ttctgaaccc gacttcaagc cgatgagctt cagcaaactg
     6781 ttgtacgtat tacttggcag cgtggtgatg atcttcgtat gtgcatggct gatgcacaac
     6841 gtagaagtcg ccaatctggt gctgattgtt ctctccatcg tcgtcaccat catcttcttt
     6901 cgtcaggcat tcaagctgga taaaaccggg cgcaataaaa tgtttgtcgc ctttgtcctg
     6961 atgctcgaag cggtggtgtt ttacattctc tacgcccaga tgccaacatc gctgaacttc
     7021 tttgccatca acaacgtgca tcatgaaatt ctcggttttt ccatcaaccc ggtcagcttc
     7081 caggcgctta acccgttctg ggtggtactc gccagcccaa tactggcagg catttacacg
     7141 catctgggta acaaaggcaa agacctctcg atgccgatga aatttactct cggcatgttt
     7201 atgtgctctc tgggcttttt gacggctgca gctgcgggaa tgtggtttgc ggatgcccag
     7261 gggctgacat cgccatggtt tatcgtgctg gtgtacttat tccagagctt aggtgaactg
     7321 tttattagcg cccttggcct ggcgatgatt gctgccctgg tgccgcagca tttgatgggc
     7381 tttattctcg ggatgtggtt cctgacgcag gctgccgcgt tcttgctggg cggctatgtg
     7441 gcaacattta ccgcggtgcc ggacaacatt accgatccgc ttgagacgtt gcccgtctat
     7501 accaacgtgt ttggtaagat tggtctggtc acactgggcg ttgcagtagt gatgctgttg
     7561 atggtgccgt ggctgaaacg catgattgcg acgccggaaa gccattaatt attcttgcag
     7621 aaagcagggt agcgttatcg ctaccctggt ttacttttac actggcgtac ctgcataaat
     7681 atcaaaccta tgccctttag tgactaccgc gtttggcgtg gcgacattcg ccagcggtgg
     7741 cgcgtagtcc gggcgcttta ccaccacgcg tttggtcgcc agcaagcgtg caggctccag
     7801 taatccatcg gcatcaagat ccggtcccac cagcgactga aagacacgca tctctttttt
     7861 cactagcgcg cttttctgct tatgcgggaa catcgggtcg agataaacca cctgcgggcg
     7921 cggggtaata tcagtcagcg ccgtcaggct ggaggcgtga attaactgca accgctcctg
     7981 caaccagccg ccgatttccg catccgcata accacgcgcc aggccgtcgt cgagcaatgc
     8041 ggcaacgact ggattacgct ccagcatccg cacgcggcag ccgactgaag ccagcacaaa
     8101 ggcatcgcgc cccagccctg cggtggcatc caccacatcc ggcaaataat cgcctttaat
     8161 gcccaccgct ttcgccaccg cctcaccgcg accaccgccg aatttgcgtc ggtgcgccat
     8221 cgctccgcca acaaaatcaa caaagatgcc gccaagtttt ggctcatcac gcttgcgcaa
     8281 ttccagatgt tccggcgtta acaccagcgc catcaggttg tcttcatcgt gctccagccc
     8341 ccagcggacc gccagaacag ataaggcacc gtctccggtg cctgtttcat caattaagca
     8401 gattttcact gaatgatcag cccttaatgc cgtaatgctc cagcatcgca tccagctgcg
     8461 gttcacgacc acggaagcgt ttgaacagat ccatcggctc ttctgaaccg ccacggctca
     8521 gaatgttgtc gaggaacgac tgcccggttt cacggttgaa aatgccctct tcctcaaagc
     8581 gcgagaaagc atctgccgcc agtacgtcag cccacaggta gctgtagtaa cctgcggcat
     8641 aaccaccggc gaaaatatgg ctgaaagcgt gcgggaaacg gccccaggat ggagacggta
     8701 ctacggcaac cagtttcttg atttctgcca gagtttcgag gattttcgct ccctgatccg
     8761 ggcggaactc ggcatggagg cggaaatcga acaggccgaa ctccagctgg cgcagaataa
     8821 acagcgccgc ctggtagttc ttcgccgcca gcattttatc cagcaactct ttcggcagcg
     8881 gttcgccggt ttcgtagtga ccggagataa acgccagcgc ctccggctcc cagcaccagt
     8941 tttccataaa ctgactcggc agttcgaccg catcccacgg cacaccgctg ataccggaaa
     9001 caccagcggt ttcgatgcgg gtcagcatat ggtgcaggcc gtgaccgaac tcgtggaaca
     9061 gggtgatcac ttcgtcgtga gtgaacagcg ccggtttacc atttaccggg cggttgaagt
     9121 tacaagtcaa atacgcgacc ggtttttgca gcgaaccatc agctttacgc atctggccta
     9181 cgcagtcatc catccacgcc ccgccgcgct tgttttcacg ggcatacaga tcgaggtaga
     9241 agctgccgcg cagttcgtta ttttcgtcat acagttcgaa gaaacgtaca tccggatgcc
     9301 agacatcaac atctttacgc tctttagcgg tgatgccgta aatacgtttc accacttcaa
     9361 acaggccgtt aaccgctttg ttttctggga agtacggacg cagctgctcg tcgctgatgc
     9421 tgtagaggtg ctgtttttgt ttttcgctgt agtacgcgat atcccacggc tgcaactcat
     9481 cgacgccaaa ttccgctttg gcgaaggcgc gcagttgtgc cagctctttt tcgccttgcg
     9541 gacgcgcgcg ttttgccaga tcggttaaga aatccagcac ctgctgcggg ttttctgcca
     9601 ttttagtggc aagggattta aaggcgtagt tttcaaagcc cagcagttgc gccagttcgt
     9661 gacgcagagc gaggatctct tccatcacct tgctgttatc ccatttaccg gcgttcgggc
     9721 cttgatcgga ggcgcgggtg ctgtaagcgc gatacatctc ttcacgcaag gcctggttgt
     9781 cgcagtaggt cattaccggc aggtagcttg ggatatccag cgtcagcagg tagccttcca
     9841 gctctttcgc ttcggcctgg gcttttgccg cagccagcgc gctttcaggc atccccgcca
     9901 gctccgcttc gtcggtaacg agtttggtcc agcccatcgt cgcatcgagg acgttgttgc
     9961 tgtactggtt gcccagctcg gaaagacgag tggcaatctc accgtagcgt tgttgtttct
    10021 ctattggcag accgatgcca gagagttcga agtcgcgcag tgcgttatca accgcttttt
    10081 tctgagccgt gttcagcgtg gcgtaatgat cgccatcgcg caggtcgcga tacgctttat
    10141 acagcccttc atgttgccct acccaggtgc tgtattccga cagcagcggc agggtttgtt
    10201 cgtacgcttc acgcagttcc gggctatttt tcaccgagtt caggtggctg accggggaga
    10261 agatacgccc cagcacatcg tccacttccg ccaacggctg gcagagattt tcccaggtgt
    10321 acggtgctcc ttgcgctact acgcgctcca cattttcgcg gcagtcgttc agcgccttag
    10381 tcacggctgg aacgacatgt tccgggagaa ttttagaaaa cggaggcaat tcaaagggag
    10441 tcagtaacgg attcgtcatt cgcgcagtcc tggttaaaga ggttaaggaa gcgctcaaca
    10501 ggcgctttac ataatgtgtg tagcatgggg ttaagtgtag tgaatttcaa tgagaaacgt
    10561 tacgctttcg cggcggcggc ctcttttcgg tatactgtcc tgatacgctt ttgtgcgccc
    10621 cgaatacggg ccgattttta cttaccggaa cacctttacc catgctcagt tatcgccaca
    10681 gctttcacgc tggcaaccac gccgacgtcc ttaaacatac cgttcagagc ctgatcatcg
    10741 agtcgctgaa agagaaagat aaaccgtttc tctatctcga cacccacgca ggggccgggc
    10801 gttatcagtt aggcagcgaa catgccgagc gtaccggcga atatctcgaa ggcatcgccc
    10861 gtatctggca gcaggacgat ttgcccgcag aactggaggc gtacatcaat gtggtaaaac
    10921 acttcaaccg tagcggtcag ttgcgttact accccggttc gccattgatt gctcgccagc
    10981 tactgcgcga acaagacagc ctgcaactga ccgaactgca cccgagcgat tacccgttgc
    11041 tgcgttctga atttcagaaa gacagccgtg cgcgcgtcga aaaagccgat ggtttccagc
    11101 agcttaaggc caaactgccg ccggtttccc gccgtggttt aatccttatc gacccgccgt
    11161 atgaaatgaa aaccgactat caggcggtgg tcagcgggat agcagaaggt tacaaacgtt
    11221 tcgccactgg tacttacgca ctgtggtatc cggtggtgct gcgtcagcaa attaagcgca
    11281 tgatccacga tctggaagcg accggtattc gcaaaattct gcaaattgaa ctggcggtac
    11341 tgccagacag cgatcgccgt ggcatgaccg cttccggcat gattgtgatt aacccgccgt
    11401 ggaaactgga acagcagatg aataacgtgc tgccgtggct gcacagcaaa ctggttccgg
    11461 caggcaccgg gcacgccacc gtaagctgga tcgtgccgga gtaattgcag ccattgctgg
    11521 cacctattac gtctcgcgct acaatcgcgg taatcaacga taaggacact ttgtcatgac
    11581 taaacactat gattacatcg ccatcggcgg cggcagcggc ggtatcgcct ccatcaaccg
    11641 cgcggctatg tacggccaga aatgtgcgct gattgaagcc aaagagctgg gcggcacctg
    11701 cgtaaatgtt ggctgtgtgc cgaaaaaagt gatgtggcac gcggcgcaaa tccgtgaagc
    11761 gatccatatg tacggcccgg attatggttt tgataccact atcaataaat tcaactggga
    11821 aacgttgatc gccagccgta ccgcctatat cgaccgtatt catacttcct atgaaaacgt
    11881 gctcggtaaa aataacgttg atgtaatcaa aggctttgcc cgcttcgttg atgccaaaac
    11941 gctggaggta aacggcgaaa ccatcacggc cgatcatatt ctgatcgcca caggcggtcg
    12001 tccgagccac ccggatattc cgggcgtgga atacggtatt gattctgatg gcttcttcgc
    12061 ccttcctgct ttgccagagc gcgtggcggt tgttggcgcg ggttacatcg ccgttgagct
    12121 ggcgggcgtg attaacggcc tcggcgcgaa aacgcatctg tttgtgcgta aacatgcgcc
    12181 gctgcgcagc ttcgacccga tgatttccga aacgctggtc gaagtgatga acgccgaagg
    12241 cccgcagctg cacaccaacg ccatcccgaa agcggtagtg aaaaatgccg atggtagcct
    12301 gacgctggag ctggaagatg gtcgcagtga aacggtggat tgcctgattt gggcgattgg
    12361 tcgcgagcct gccaatgaca acatcaacct ggaagccgct ggcgttaaaa ctaacgaaaa
    12421 aggctatatc gtcgtcgata aatatcaaaa caccaatatt gaaggtattt acgcggtggg
    12481 cgataacacg ggtgcagtgg agctgacacc ggtggcagtt gcagcgggtc gccgtctctc
    12541 tgaacgcctg tttaataaca agccggatga gcatctggat tacagcaaca ttccgaccgt
    12601 ggtcttcagc catccgccga ttggtactgt tggtttaacg gaaccgcagg cgcgcgagca
    12661 gtatggcgac gatcaggtga aagtgtataa atcctctttc accgcgatgt ataccgccgt
    12721 caccactcac cgccagccgt gccgcatgaa gctggtgtgc gttggatcgg aagagaagat
    12781 tgtcggtatt cacggcattg gctttggtat ggacgaaatg ttgcagggct tcgcggtggc
    12841 gctgaagatg ggtgcaacca aaaaagactt cgacaatacc gtcgccattc acccaacggc
    12901 ggcagaagag ttcgtgacaa tgcgttaaat gttaaagggc taagagtagt gtgctcttag
    12961 cccttaatta cgtttccgct atcagttcag aagctgaagc agaaagcgga tcagttccag
    13021 cagcgcaatt aacgccccaa gaacgatgat tgctttatca atcacccgtt ttctccatgc
    13081 gatggagtga gaatgcatcc gcttactcat ccactgcctg tcacggcgca tgccctattg
    13141 ttagataatg gcattctcac tccggccaga gcatcagtta acggcaccac ccgtacttct
    13201 gaccaggact ttgaaagcgt ttatgcgcat taccagagtg aaaatgcctc agagctaact
    13261 ggataatcat acagtacatt caggttataa aaccagcatg tccttgcaat agtttcagta
    13321 tggtattagc attgatgcgt tagataatgg cattctcact ccagccagtg ccaccaactc
    13381 agggctggaa agtaaaaaac cgacgcaaag tcggtttttt tacatccgga ttcggacaag
    13441 gcttaatatg acgatgaccc agtgaaagta tataaatcgt cattgcgata tataccgaag
    13501 tgccccctcc gccagctgaa gaaatcgcta attcttgcaa tgttagccac tggctaatag
    13561 tattgagctg ttagataaga actctctcac tccagccaga gccaccaact cagggctgga
    13621 aagtaaaaaa ccgacgcaaa gtcggttttt ttacgtcctg attcagacct cctttcaaat
    13681 gaatagccaa ctcaaaattc acacctatta ccttcctctg cactta
//
LOCUS       DAFAVF010000112        13549 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFAVF010000112 DAFAVF010000000
VERSION     DAFAVF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.112"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(17..760)
                     /gene="traF"
                     /locus_tag="LGK42_003204"
     CDS             complement(17..760)
                     /gene="traF"
                     /locus_tag="LGK42_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBK2659427.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     IRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(753..1010)
                     /gene="trbE"
                     /locus_tag="LGK42_003205"
     CDS             complement(753..1010)
                     /gene="trbE"
                     /locus_tag="LGK42_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBK2659428.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(1037..2845)
                     /gene="traN"
                     /locus_tag="LGK42_003206"
     CDS             complement(1037..2845)
                     /gene="traN"
                     /locus_tag="LGK42_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBK2659429.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGGDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(2842..3480)
                     /gene="trbC"
                     /locus_tag="LGK42_003207"
     CDS             complement(2842..3480)
                     /gene="trbC"
                     /locus_tag="LGK42_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBK2659430.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3489..4481)
                     /locus_tag="LGK42_003208"
     CDS             complement(3489..4481)
                     /locus_tag="LGK42_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBK2659431.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPALPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4478..5110)
                     /gene="traW"
                     /locus_tag="LGK42_003209"
     CDS             complement(4478..5110)
                     /gene="traW"
                     /locus_tag="LGK42_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBK2659432.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5107..5493)
                     /gene="trbI"
                     /locus_tag="LGK42_003210"
     CDS             complement(5107..5493)
                     /gene="trbI"
                     /locus_tag="LGK42_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBK2659433.1"
                     /translation="MSSTQKPADVMAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDRASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(5490..8117)
                     /gene="traC"
                     /locus_tag="LGK42_003211"
     CDS             complement(5490..8117)
                     /gene="traC"
                     /locus_tag="LGK42_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBK2659434.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8277..8498)
                     /gene="traR"
                     /locus_tag="LGK42_003212"
     CDS             complement(8277..8498)
                     /gene="traR"
                     /locus_tag="LGK42_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBK2659435.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPV
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(8633..9148)
                     /gene="traV"
                     /locus_tag="LGK42_003213"
     CDS             complement(8633..9148)
                     /gene="traV"
                     /locus_tag="LGK42_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBK2659436.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9145..9396)
                     /gene="trbG"
                     /locus_tag="LGK42_003214"
     CDS             complement(9145..9396)
                     /gene="trbG"
                     /locus_tag="LGK42_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HBK2659437.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(9408..9605)
                     /locus_tag="LGK42_003215"
     CDS             complement(9408..9605)
                     /locus_tag="LGK42_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBK2659438.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     SESLPDNFNMTAVGVMKNSEI"
     gene            complement(9602..10174)
                     /locus_tag="LGK42_003216"
     CDS             complement(9602..10174)
                     /locus_tag="LGK42_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBK2659439.1"
                     /translation="MANNTSSRQAGHAARYVVARVLRGLFWCLKYAVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPVRPGKMKCVRVATADERLKEV
                     LAEDTSLPGKIRECHVYFPDDRTIRNNGEK"
     gene            complement(10164..11591)
                     /locus_tag="LGK42_003217"
     CDS             complement(10164..11591)
                     /locus_tag="LGK42_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBK2659440.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDISGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(11591..12295)
                     /gene="traK"
                     /locus_tag="LGK42_003218"
     CDS             complement(11591..12295)
                     /gene="traK"
                     /locus_tag="LGK42_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBK2659441.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTSYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(12306..12872)
                     /gene="traE"
                     /locus_tag="LGK42_003219"
     CDS             complement(12306..12872)
                     /gene="traE"
                     /locus_tag="LGK42_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBK2659442.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(12894..13205)
                     /gene="traL"
                     /locus_tag="LGK42_003220"
     CDS             complement(12894..13205)
                     /gene="traL"
                     /locus_tag="LGK42_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBK2659443.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(13220..>13549)
                     /gene="traA"
                     /locus_tag="LGK42_003221"
     CDS             complement(13220..>13549)
                     /gene="traA"
                     /locus_tag="LGK42_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBK2659444.1"
                     /translation="PVKKKSFFSKFTRLNMLRLARAVIPAAVLMMFFPQLAIAAGSSG
                     QDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAGFAIISVFIAV
                     GMAVVGL"
BASE COUNT         3040 a         3856 c         3332 g         3321 t
ORIGIN      
        1 taaatcaaat ccgcgattaa aaattgggtt taaaatcttc agaaacgttc aggaactgtt
       61 ttgccaggtc gtcctgcgaa ataaagccgt atgataacgg gcgaatactg ccctgtttcg
      121 ggtcaaccag catcatggcc gggaaatatt tcacgccgag gcgctgcgcc tgcccctggt
      181 cagtccggga atccggcaac agcggattaa tcacgccatc cacggaaacg ggaataacac
      241 tcagaccata cgtatcccgg aagccattaa tgacctgcgc cagttgcccg tcgatggggt
      301 cctgcccccg gtaaaaaaac atgatgccgt agtgttcagc cagtttcgca atggcctgtc
      361 gctgctgcgc ctggtctgct gccagctggt tccgtaccgt gccgttgtaa tggctgtact
      421 gcaggttata gtccagttca ggatgtgcca gcatggcctt tctggcgctc atggtgaaaa
      481 gcccggcctg ctgagtccag taattttgca gccggaaata tttcacaaaa ttatccacgc
      541 cgggatacag aatggcttcg tacagcgccc gctttgttgc cgtctgcagt gcggccagtt
      601 tctgcataat atccggttcc tgacgggggg ctgcaggtac aggtttattt tctttttcct
      661 tcggatttat tttctcgtta taccactgcc agcctgcatc ttttcctgac gccggaaaaa
      721 taaagcagca gagtaacagt ggcagtaatg ctttattcat cttcattcct ttcatcactt
      781 tttctttcat caggtttctg caggtcagtc agggcagaaa aacaaaaata cagaataaac
      841 aggagcacac cggagtaaag gataacgaaa gcagcggcaa gcatcccgtc tgccgtggtt
      901 tcttttaccg tatcccacga ccagataatt accggagaaa tgacaatcgc tgtaatcaaa
      961 agccggataa gaaaatcaat aaaacggttc gacgtgaata tgactttcat catttccccc
     1021 ggttatttcc cataccttac tgtcctgcct gtttcagctg gtcagcaatc tgctctttca
     1081 ctttctgcgt cagaacgcca ctgtccggga ttttctggtt attcatcagg tcttcgtaga
     1141 agttagtgaa atccagtctg ttgaactgaa ttttctgcag ctcatcaacc gtaatccccc
     1201 gacagtcagg atgcttcgca ctaccaaaac tgatacgcaa ctgaccgttg cgcccctgct
     1261 gctggactat ctgcgccagc ttcgaatcaa actgacagta gctgcgtttt ttctccagac
     1321 agacacccag cactttcttc gaacagaact ccccgacgct gacggtcagt ttgtttgatt
     1381 ttgctttagc cagggccttt tcatcactgc tgcatttggc cagcccgata tcctgtcccc
     1441 agccgctgtc cttacagcag ttgctgtatc cggctgcagc cttcttacaa aatttcgcct
     1501 gaccggtgaa agcacggaca tcccccccgt tcagcgccgc aacatcctta cctgctgcgg
     1561 ccagtgccgc cagctgagaa acggcttcgg caaaatcatt actctgcccg ctctgagcct
     1621 tatcgcattc gccatcgaga cagaatacgt ctcccccaca gaccatcact ttcccggatg
     1681 tttttgactc acaggagtac gtggcatatt catgcagaca ggcaccttct tcggagtaaa
     1741 aggcacactg gtgtgacacc agggtacagg caggattgtc ggtgtaggtc tgacaggttc
     1801 ccttgtctgc tgactgcgtg acatacgtgt cccggtatgc ccagcagctc tggtacatgc
     1861 tgtatgactg accgtctttc accagcgtcc tgttcccgcc agcctccgta cattctgttt
     1921 ttgtcagttt ccccagctcc ttgctgaacg ggcagctttc agaccagaca acatacgggg
     1981 tgtacgaggc tttttttacc ctgacaacca gacgcagctt cattgccgta tgacgtttga
     2041 attttgtatt cccgacccgg acggggtgag atgacgtaaa agccacaccg gcagtcagtg
     2101 ttttctgtac tggtgtaaat gaggcatctg ctgagtattt ggcattccag ctcagtgggg
     2161 ttcccagtac tgtaattgtc ataggtacac tcttctgcaa gtaagtacga ctccagctgt
     2221 aatgcgcatc gacaatttca ccggtaacgg gagaggtaat ggagacaaca tactgaccat
     2281 tcacttcacg tgcaggtagc tgactcattt catactccag cgtccgggtt tcccatgtgg
     2341 tactcccctg cagctccata cgggcagttc gtgtgcagta ctgctccacc tgtaaatccc
     2401 gctcacaggt gtaattcgtg tattcactcc tgctaatctc ctgcgcactg cactgctgcc
     2461 cggtgtttcc cacaatgctg tcagcccggt tcaccacatc cctgccggtc tggataaacg
     2521 gtgcatccgg tgaaagaatg tctttcggct tgttcatgaa tgactctgta atggtttttc
     2581 cggtttcacc ggtcgcccat tccgtggtgc cgtcattttt caggccgcca tccccgccgg
     2641 cagtcacgcc accgtaatat tttgtctcgt ccggattcgc gttatagccg gggatactct
     2701 cctgtggctt aaagccctga atactgctgc ttccctgccc tttgatctgg tgagcaaaat
     2761 cagaaccggc ccggtaatca ctgttgctgt cagcctgtgc catgccggca accagagcca
     2821 gtatcagagg taaaatacgt ttcatttccc ggaatctcct ttccctgcca gtaaatcatg
     2881 agccacctgc cggcagtcac ccgtggcggc cactttctcc agcgcctggc cgacccgcag
     2941 gtttccccgg atgatgtcgt atccctgact gcagaacacc accagtgccg gtacagtgcg
     3001 aatgccgtac tgtgaaaaca gcgtcgggtc gatctgaaca ccatcagtcg cgccgtcttt
     3061 caccagggac aacacagctt cagcggtggt cttcaggtca ttgttcacca tgccccgcag
     3121 tgtggccgga ataccgtagt gccgggtttc gcccagcatt cgtttcagcc cctcttcggg
     3181 aatggaaaac gacacaaaat acagagcacc ctgccggggt ttcccgtcct gcgaagcctg
     3241 ctgtttacga accagctcat ccaggaaatg gttatctgaa cgctgaaggg ggttttccag
     3301 cacctgtttc tccgcccagg ctttcagctg atggtcaggt ttttcacgca gttgtctgct
     3361 taaattttcc tgctgcttca ggaactggcg gttttcagga gtgttcacgt tttctgacgc
     3421 cataaccgcc ccgttcagca tcatcagcag tgctgccaga gatttcatac tcagcttcat
     3481 cattcacctc acaggaagac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
     3541 tttgtgttgg gcggattttt cccggtttcc cagcgggtca cgctgcgccc gaacgggtgg
     3601 cactgcccgc tgtccggata catattcacc atctggtaac gccagcgttc tttgggcagg
     3661 attggggacg gatattcatt acagacggcg ttattttttc cgatggtttc cataatcatg
     3721 ccctgacggt gcagcttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
     3781 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
     3841 cagaacagaa catcgagagg catattaaag gcgctggcaa tcgcatctgc tgcgcaggct
     3901 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
     3961 ctgtccgtcc aggtggggtc gatttcagaa agataagcga tatccaggtc accaccttcc
     4021 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
     4081 acgtgataga acgccccatt tacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
     4141 ttaccaaaag ccggcaggct gaagcccagg ttcaccatgc atcccggtga ccgggtgacg
     4201 tccgtcaacg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
     4261 ggcagtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttcccc
     4321 tgactgactt tgatactgcc cagcgagagc gggaaaatac agctccagca gatatctgtg
     4381 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgccgcagg gacatgaccg
     4441 gcgaaaagga ataacatcag cagccacagc cttcgcttca ttttctgccc tcctctgccg
     4501 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
     4561 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
     4621 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
     4681 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
     4741 gcgtctggtt aaaaggaaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
     4801 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
     4861 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
     4921 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
     4981 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
     5041 aggtaccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
     5101 ggcatctcat ggttccgccc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
     5161 atccggtgcc ccctgtacca cagcaggcga caccagaatg accgcgtggt gtttctgctg
     5221 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
     5281 cgcttctgac agttgtttct gactggctct gtcaaaaaac gcatccacgg tctgtttcat
     5341 gttgaaggcg acagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
     5401 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcgttctgc
     5461 catgacgtcg gcgggtttct gcgttgaact catgccacac tcctgtattt ctcatgttct
     5521 tccagccagg cttccagcga agccatttcc ggccctgact tcttccacgc cagctgccac
     5581 actgcctcat gaatactcag cccctcttta cgcttctgct gcacaaactc aaaatccggg
     5641 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
     5701 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaactta
     5761 ccaatcatgt cgcgctgcag tggcaggaac tggtccggat acagttggtt gtatttcgcg
     5821 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcgcgg
     5881 gcagcactgg aggccttatc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
     5941 gcaccggtat gacggcgggc cgtacggtag cctttctcaa taaattcacc gaccttgtga
     6001 tttttgaagt ccagcagacg ccagccttca tcaataacgt tcagtttctt gaggttacgc
     6061 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
     6121 agtgacggac ggtcttccag tccgcccagc tccagcacca ccatttttgc gtcatcccgc
     6181 agggacggct catcagagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
     6241 agcagcacaa tcatttcgtc cagacggctg cggatagtcg gtgactcggc atactgctcg
     6301 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgctct gttctctttg
     6361 gccagccagg aggctctgac cgcctgcaac agcagacctt catgcacttc atccaggtta
     6421 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
     6481 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata caccccgccc
     6541 atgttctcac acagggactt gtatccatcc cccatgtcga acaccacggc aaagcctccg
     6601 gagtccagca cgctgcggat aagtggctgt atcagcccgg ttttaccggc cccggaggtg
     6661 ccacagaccg ccatgttgta gttggtgtta ttcatccccc ggaagaaaat atcgataaac
     6721 gccagctggt tacggtaggt gggtgccagc agacctgccg gtgtcagggg gttatccgcc
     6781 actaacggca tcaggttggc aacattaaag ctctctgcgc gctgaaccac tccggcctct
     6841 ttcaactgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
     6901 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacgaaagct gttcaggatg
     6961 tcctgctcca cttccagcgc cgtttcatta ttgtccttgc agaatgctgt gatgttgagg
     7021 aagtaggaca ccacggagga ctgaccggag cccagccgct gacgcagttc cccccactcc
     7081 ttcgcctctt tctccacgga cggaaaccat ttggcatagg aggtcttcga ctttttctcc
     7141 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
     7201 gtcagcgtca ggatgaacgg acaggagatg gacagttccg ggttcagcag gttgctgtag
     7261 ttgtcggcca cgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
     7321 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
     7381 actttcagat caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
     7441 tgacgtcttt tcgggtacag ggagtccggg ttatggttaa tcatctcccc gacaatatcg
     7501 ataaaggcct gtgcatccac cgtctgtgtg gtgatactgg ccccctgtaa cgacgcccgg
     7561 atgattttca ccaggttttc catttccaga atgtcggccc ggcttttttt cttcgaagga
     7621 gaacagtacg agataaacac ccggtaatgg cgcagggtca ggggcagatt catcccctcc
     7681 ggcagcggaa actgtgtcgc tgccgctttc atataatagg cccgggtaat ggcgttaaac
     7741 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
     7801 acaagctgac tggacatcag atggatgcac aacggaatac cgcgcggcag tttggtgcgc
     7861 agcatgtgat cgagggcctc cacaatggac tcattcgccc cattgatggg gatggcttcc
     7921 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
     7981 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
     8041 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
     8101 tcaagtgggt tattcacatg aattccttag ttgaaaaata aagcgagaaa tgcggtagcc
     8161 atgaagggaa tacctgtaat taacgcgcca tttcccggcg catatacaaa gatttctgtt
     8221 ttctgaatat ccactcatgt tcatccggaa ttaaatcact atgttctgcg actgacttat
     8281 gcataatgtt ttctctgtct ttcctgatac gcctgacatt caacgcacaa cgtcacaccg
     8341 ggaaatattt tccgccgggc ttccggaaca ggatttccgc atgcttcaca gagataaaca
     8401 ggaataccat gagcatttat tttctggcgt atccggttta ttcctgtcat ggtcagttgt
     8461 tctgtcactg aatatgcttc atcggcttca tcactcacgg aatattcctc ccgacatgtt
     8521 ctgacacgct accgcccatc ccttttcacg cacagggcac tcgttttaca ggcagatttt
     8581 gaatacagaa agcccgccgg cattgccggc tgatatacaa cttcagggcc agttaattaa
     8641 tacgtggttt tccccacgca gacggtttaa taacaaaaaa cacgctggat ggctgatgat
     8701 aaacatcctg attatcaata taaggcgcta tccataatgc agccgtctgc tcacccgttc
     8761 ttagcggacg aggaggtgtt accggcgcaa ctgaactgac cggaacaatc gttttcactt
     8821 cccgggcagc ggtaaacagc gggcgtggtg ccagcagttt ctgctccgga tgtgctgtca
     8881 ccgcaggtct gctgccggac ggagtggtcg cggtgacggt ctgcaccggc attgtccgga
     8941 agttcccttc agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct gaggactgct
     9001 ccagtttttt ggccttctca ttggcctgct ccatcgtcat acaggtatcg gatgtggtgg
     9061 cgttacactc aaattccgta ctggttccgg cacagccgga aagtaataag gtccccagca
     9121 gaggaataaa taaagaaatc tgtttcattg tccctcacct ttctcatcgc agttcacaaa
     9181 atccttcttc aaatccctga ccgtacaggt ttttacaatg tcaggatttt caaaaaccgt
     9241 actccgtgat gatttataaa catatgccac aatgcgtttc ccacccgcat caggttctgg
     9301 tgcactgact tcacataacg gaggtgttat tccggactca tacagcaccg gataatttat
     9361 ttttttgacg ctgccatcag aaaccagctt attcatcgaa gggttcctta tatttctgaa
     9421 ttcttcatca caccgacagc agtcatatta aaattgtccg gcaatgactc agaaccgaat
     9481 ttctccggtg caggaagtac gattcttgtg gcgcagttac tgagaacagc atgcataaaa
     9541 tcatctgaca cggttttacc cggcacagaa agggctgtaa taacattaat attacgcatg
     9601 ttcatttttc tccgttgttt ctgattgttc tgtcatcggg gaaataaaca tggcattccc
     9661 tgatttttcc cggcagggaa gtgtcttctg caagaacttc cttcagacgc tcatctgctg
     9721 ttgccacacg gacacatttc atttttccgg gtcgcaccgg atatttcccg gtgcctgccg
     9781 caaacgacat atacataagt gccagcgttg cccagaatgc ggtgaccagt tgcatcagtg
     9841 accggtcagt ctccctgaca taatctgcag catcagcagc acggtaacgg cagattttct
     9901 cttctgctcc tggaagatca tgcagagaag gcaaacattc tcttacagga aactgcccgg
     9961 caggggctgt ttgtcttacg aagtcaattt cctgaaccag taattttcct ggtgtggtgt
    10021 tgtccttcca taaaacaaac agagccatca gggccattgt ggccagtggc agaatgacgg
    10081 cgtatttcag acaccagaac agtcctcgca gaacccgggc gacaacatac cgggcggcat
    10141 ggcccgcctg acgggacgac gtattatttg ccatcattgc cccctccctg actgccggac
    10201 ataaactgct gcggattcag gttgcccagc tgcttcagca tctggtcagt attaaagccg
    10261 ttaagatgac tttcagctga tggcggaaca ggaaccggac tttccggatt gtcttcttcc
    10321 gctctgctct gcgtgcgttc cagcgccatc tcttccacgg ttttcagctg gaagccgtcc
    10381 tggaacacca ctgtcacttc gttgcccgca ccaatcggta tcaccgggtg atactgttcg
    10441 gcacgtttga tgtagtagtc actgagcgtc tgtgcggctt tcgatgcgcc gccaccgata
    10501 cccattttaa ggacatcgcc ggccccgtaa gcagcagtgg cacccagccc gacagccggc
    10561 tgggaggcgc gctccattcc ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag
    10621 ccgaggattt tgccgttacg catcaccact tcgcccttga taccgttttt accccggaag
    10681 ctgacatgcc ccttaatcgg catatcaata gttttgccgt ccttcaggca actgatattg
    10741 cgggtgcgta caatggcacg ctcactggac acatcccccc aggcttccag acccacaaaa
    10801 catcccgttg cgtcatacgt cttgctgttc ggcatttcca ccaggccggt gatacgcagc
    10861 tgcatcggca ccgtggattc attaccggtg acagaggcat tggcatccgc cccttcaatc
    10921 agcatggctt tcgcaaaact tcctgacgga atgtacggca gtgatggtcc ctgttttccc
    10981 tcattacggg taaacacctt acgctgtatc cggttaggca ccggcacaga ctggtacgtc
    11041 acctgtggtg gtggcgtgac accattcccg ggataaaacg ccgtcggcgg tggaacagca
    11101 acgctgccct gcggcgggaa ggatacgggg gtgtttcctg gctgtggttc gccttccggg
    11161 ccgggcggtg aggcaggcat ctgcggtaca ggctcaccgg tcgccgtgac gggattagca
    11221 cccagggctt ttacctgctc tgccagggcg gcattgtcct gtcccagctt ttcaatacga
    11281 cgctggtcat caccgcgctg tttgttcaga acatccagct cacgacggat ttcctcatac
    11341 tgcttctgca tctgcgctgc cgtcacctgc atctctgtgg tggcatgctg acgcaccttg
    11401 tcatcaaagg tcgtatccac cacaccggtc atatccggca caggctcctg ttcagccacg
    11461 gtttcaccgt taccggatat gtccacatca gacagataca gtgccccacc aatcgcggag
    11521 gctgtaccga caaccacaat ccccagccac aggtactgct tgcgtttcac aatggtattg
    11581 atactggcca tcagttgccc tccccgtcac ggatgacata cacatccatg cgcgcacctg
    11641 ccagtaactg gctggcaggc tggctgaaca tcacggcacg ggttcccggc tgccagaagt
    11701 cactttcccg gatattcagg gcagacagcg ttttattttc cacggcaaag cggaccatct
    11761 tcaggtgatt ccccgtccat acggcatctg ccactgaaga cagccccgcc ggggcctgca
    11821 gggtttcctt tgtcactggg acctgatacc agcctgcggg taattttccg ccacggacgg
    11881 cctggctgat ggtcacaagc agggattcgt aagacgtgga cgtttcccac gcaccggctt
    11941 cttctccggt tccgcgcagg tcactgacca gctgaatggt acgccccgcg ccttcacggg
    12001 gaacggcctg aatggaaaga ttcagaccac gttctgtttc cagaatgaac gtaaagggct
    12061 ttttgttgac ggtggcaacc accacaccgc cggaggcggt ctgctcatta ttggtcaggg
    12121 caccatccag actgttcacg gcgataatcc ggtcaccggg aacggtaaac agattcgggc
    12181 tggtattgct gatggcaata ctggcctgac caccgttcac cattggcacc acggtggggg
    12241 ccagcgtacc gtttgcggcc atcacgctgc aggaaaaaaa caggctgccg aatattattg
    12301 ccgtattatt ttttctcatc gtctgtttca ccgaaattat ccagccaggt caccccgttt
    12361 tcccgcttca gaataaggat gtaatgtttg atatcagtga aaggttttga atcaccaatc
    12421 caggttttaa gcacacctcg aatttccaca cggccatact gaggccagac acgaacactg
    12481 gtctggaaaa aggctgagtt cacgttatcg tttttaatac gcttcgcttc ttcagccaga
    12541 ataactttca tctggttctg tgcgcccggg cggatatatt gcagaagcgc ctgatgtgag
    12601 gcatcgacgg tttctgatga aacattcaga cggagggcaa taaatgacag cgccatctgc
    12661 tgtaaataag aggcgtcggc actgttctgt gacacggcaa agggggcatt aaatgccatt
    12721 ggcgtcacgg cagtgcgttg ctcattctga agacggtagt tattcacccc ctgaatgacg
    12781 ttaacagaga ggctgagaac aataagcact gacataaata taaaggcgat ggccattaca
    12841 cgactggtac ttaaacgggc accgtgttcc atataacaat cctggtatca gttctattta
    12901 atccactgcc ggaaacacga atcgggaaca ttatgaaaaa taccgcgcag cagggctgtt
    12961 ggcatatacc agtaaatcag gtcacgtaac caggaactgc cccgcccttt tttcagtttt
    13021 ttaatcccga aataaaccag aaccgctgca ccaataccga acagatattt cgatgttgtg
    13081 ataccccagc caatacagat tgctgcgggg atcagttcat ccagtggcag gccaaaccag
    13141 cggctctggt tggtcagtgt ttccgggaaa cgatatttct taagtttatt ctcgtctccc
    13201 gacatcgttt tatttcctgt cagaggccaa cgacggccat acccacagca ataaatacag
    13261 agatgatggc aaaaccggcc aggaacttga cgtttttggt catcatgtac atgacagcac
    13321 cgaccagaac ttcagccaga acaacccatt taacaacact ggagtcctta ccgaaggtcg
    13381 ccttaaccgt ggtgttaccg cttgccatca ggtcctgacc actgctgccg gcggctatcg
    13441 ccagctgcgg gaagaacatc atcagaacag cagccgggat cactgcgcga gccaggcgaa
    13501 gcatattcag acgagtgaat ttagaaaaaa acgacttctt tttgacggg
//
LOCUS       DAFAVF010000113        13378 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFAVF010000113 DAFAVF010000000
VERSION     DAFAVF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.113"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            72..4034
                     /gene="putA"
                     /locus_tag="LGK42_003222"
     CDS             72..4034
                     /gene="putA"
                     /locus_tag="LGK42_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2659445.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4074..4712)
                     /gene="rutR"
                     /locus_tag="LGK42_003223"
     CDS             complement(4074..4712)
                     /gene="rutR"
                     /locus_tag="LGK42_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBK2659446.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            5000..6091
                     /gene="rutA"
                     /locus_tag="LGK42_003224"
     CDS             5000..6091
                     /gene="rutA"
                     /locus_tag="LGK42_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBK2659447.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            6091..6783
                     /gene="rutB"
                     /locus_tag="LGK42_003225"
     CDS             6091..6783
                     /gene="rutB"
                     /locus_tag="LGK42_003225"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBK2659448.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6795..7181
                     /gene="rutC"
                     /locus_tag="LGK42_003226"
     CDS             6795..7181
                     /gene="rutC"
                     /locus_tag="LGK42_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBK2659449.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            7189..7989
                     /gene="rutD"
                     /locus_tag="LGK42_003227"
     CDS             7189..7989
                     /gene="rutD"
                     /locus_tag="LGK42_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBK2659450.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            7999..8589
                     /locus_tag="LGK42_003228"
     CDS             7999..8589
                     /locus_tag="LGK42_003228"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBK2659451.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            8600..9094
                     /gene="rutF"
                     /locus_tag="LGK42_003229"
     CDS             8600..9094
                     /gene="rutF"
                     /locus_tag="LGK42_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBK2659452.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9115..10443
                     /gene="rutG"
                     /locus_tag="LGK42_003230"
     CDS             9115..10443
                     /gene="rutG"
                     /locus_tag="LGK42_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBK2659453.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(10526..10699)
                     /locus_tag="LGK42_003231"
     CDS             complement(10526..10699)
                     /locus_tag="LGK42_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBK2659454.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            11072..11668
                     /gene="wrbA"
                     /locus_tag="LGK42_003232"
     CDS             11072..11668
                     /gene="wrbA"
                     /locus_tag="LGK42_003232"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBK2659455.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            11689..11916
                     /locus_tag="LGK42_003233"
     CDS             11689..11916
                     /locus_tag="LGK42_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBK2659456.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(11954..13195)
                     /gene="agp"
                     /locus_tag="LGK42_003234"
     CDS             complement(11954..13195)
                     /gene="agp"
                     /locus_tag="LGK42_003234"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBK2659457.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         2953 a         3549 c         3684 g         3192 t
ORIGIN      
        1 caaagttgca acatcatgga tatttcacga taacgttaag ttgcaccttt cagaacaaca
       61 ggagtaatgg catgggaacc accaccatgg gggttaagct ggacgacgcg acgcgtgagc
      121 gtattaagtc tgccgcgaca cgtatcgatc gcacgccaca ctggttaatt aagcaggcga
      181 ttttttctta tctcgaacaa ctggaaaaca gcgatactct gccggagcta cctgcgctgc
      241 tttctggcgc tgccaatgag agcgatgaag caccgactcc ggcagaggaa ccacaccagc
      301 cattcctcga ctttgccgag caaattttgc cccagtcggt ttcccgcgcc gcgatcacag
      361 cggcctatcg ccgcccggaa accgaagcgg tttctatgct gctggaacaa gcccgcctgc
      421 cgcagccagt tgctgaacag gcgcacaaac tggcgtatca actggccgat aaactgcgta
      481 atcaaaaaaa cgccagtggt cgcgcaggta tggttcaggg gttattgcag gagttttcgt
      541 tgtcatcgca ggaaggcgtg gcgctgatgt gtctggcgga agcgttgttg cgtattcccg
      601 acaaagccac ccgtgatgcg ttaattcgcg acaaaatcag caacggtaac tggcaatcac
      661 acattggtcg tagcccgtct ttgtttgtta atgccgccac ctgggggctg ctgtttactg
      721 gcaaactggt ttccacccat aacgaagcca gcctctcccg ctcgctgaac cgcattatcg
      781 gtaaaagcgg tgaaccgctg atccgcaaag gtgtggatat ggcgatgcgc ctgatgggcg
      841 agcagttcgt cactggcgaa accatcgcgg aagcgttagc caatgcccgc aagctggaag
      901 agaaaggttt ccgttactct tacgatatgc ttggcgaagc tgcactgact gccgcagatg
      961 cacaagcgta tatggtttcc tatcagcagg cgattcacgc cattggtaaa gcgtccaatg
     1021 gtcgtggcat ctatgaaggt ccgggcattt caatcaagct gtcggcgctg catccgcgtt
     1081 acagccgcgc ccagtatgac cgggtaatgg aagagcttta cccacgtctg aaatcactca
     1141 ccctgctggc gcgtcagtac gatattggta tcaacattga cgccgaagag gccgatcgtc
     1201 tggagatctc cctcgatctg ctggaaaaac tctgtttcga gcctgagctg gcaggctgga
     1261 acggtatcgg ttttgttatt caggcttatc aaaaacgctg cccactggtg attgattacc
     1321 tgattgatct cgccacccgc agccgtcgcc gtctgatgat tcgcctggtg aaaggcgcgt
     1381 actgggatag tgaaatcaag cgtgcgcaga tggacggcct tgaaggctat ccggtttata
     1441 cccgcaaggt gtataccgac gtttcatatc tcgcctgtgc gaaaaagctg ctggcggtgc
     1501 cgaatctaat ctacccgcag ttcgcgacgc acaacgccca tacgctggca gcgatttatc
     1561 aactggcagg tcagaactac tatccgggtc agtacgagtt ccagtgcctg catggtatgg
     1621 gcgagccact gtatgagcag gtcaccggga aagttgccga cggcaaactt aaccgtccgt
     1681 gtcgtattta tgctccggtc ggtacccatg aaacgctgtt ggcgtatctg gtgcgtcgcc
     1741 tgctggaaaa cggtgctaac acctcgtttg ttaaccgtat tgccgacacc tctttgccac
     1801 tggatgaact ggtcgccgat ccggtcactg ctgtagaaaa actggcgcaa caggaagggc
     1861 aaactggatt accgcatccg aaaattcccc tgccgcgcga tctttacggt cacgggcgcg
     1921 acaactcggc agggctggat ctcgctaacg aacaccggct ggcctcgctc tcttctgccc
     1981 tgctcaatag tgcactgcaa aaatggcagg ccttgccaat gctggaacaa ccggtagcgg
     2041 caggtgagat gtcgcccgtt attaaccctg cggaaccgaa agatattgtg ggctttgtgc
     2101 gtgaagccac gccgcgtgag gtagaacagg cgctggaaag tgcggttaat aacgcgccaa
     2161 tctggtttgc cactcctccg gttgaacgcg cggcgatttt gcatcgcgct gccgtgctaa
     2221 tggaaagcca gatgcagcaa ctgattggta ttctggtgcg tgaagccgga aaaaccttca
     2281 gtaacgccat tgccgaagtg cgcgaagcgg tcgattttct ccactactat gccggacagg
     2341 tgcgggatga tttcgctaac gaaacccacc gtccattagg gcctgtggtg tgtatcagtc
     2401 cgtggaactt cccgctggct attttcaccg ggcagattgc tgccgcactg gcggcaggta
     2461 acagcgtgct ggcaaaaccg gcagaacaaa cgccgctgat tgccgcgcaa gggatcgcca
     2521 ttttgctgga agcgggtgta ccgccaggcg tggtgcagtt gctgccaggt cagggggaaa
     2581 ccgtgggcgc gcaactgacg ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa
     2641 ccgaagtcgc tacgttactg cagcgcaata tcgccagccg cctggacgct cagggtcgcc
     2701 ctattccgct catcgctgaa accggcggca tgaacgcgat gattgtcgat tcttcagcac
     2761 tgaccgaaca ggtcgtcgtg gatgtactgg cctcggcgtt cgacagtgcg ggtcagcgtt
     2821 gttcggcgct gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc
     2881 tgcgcggcgc aatggccgaa tgccggatgg gtaatccggg tcgcctgacc accgatatcg
     2941 gtccggtgat tgatagcgaa gcgaaagcca atattgagcg ccatattcag accatgcgta
     3001 gcaaaggccg tccggtgttc caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc
     3061 aaagcggcac ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc gaattgcaaa
     3121 aagaggtctt tggtccggtg ctgcatgtgg tgcgttacaa ccgtaaccag ctaccagagc
     3181 tgatcgagca gattaacgct tccggctatg gtctgacgct tggtgtccat acgcgtattg
     3241 atgaaaccat cgcccaggtc actggctcgg cccatgttgg taacctgtat gttaaccgta
     3301 atatggtggg cgcagtggtg ggtgtgcagc cattcggcgg cgaagggttg tccggtaccg
     3361 ggccgaaagc aggcggtccg ctctatctct accgtctgct ggcgaatcgc ccggaaagtg
     3421 cgctggcagt gacgctcgcg cgtcaggatg cagagtatcc ggtcgatgca cagttgaaag
     3481 ccgcattgac tcagccgcta aatgcactgc gggaatgggc agcaaatcgt ccagaattgc
     3541 aggcgttatg tacgcaatat ggcgagctgg cgcaggcagg aacacaacga ttgctgccgg
     3601 ggccgacggg tgaacgcaac acctggacgc tgctgccgcg tgagcgcgtg ttgtgtattg
     3661 ccgatgatga gcaggatgcg ctgactcagc tcgccgccgt gctggcggtg ggcagccagg
     3721 tactgtggcc ggatgacgcg ctgcatcgtc agctagtaaa ggcattgcca tcggcagtca
     3781 gcgaacgtat tcaactggcg aaagcggaaa atataaccgc tcaacccttt gatgcggtga
     3841 tcttccacgg tgattcggat cagcttcgcg cattgtgtga agccgttgct gcgcgggatg
     3901 gcgcaattgt ttcggtgcag ggttttgccc gtggcgaaag caatatcctt ctggaacggc
     3961 tgtatatcga acgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagtttaa
     4021 tgactatagg ttaaacaggt agccggagga tgttacaacc tcctccggca tctttaacgt
     4081 ggtcgaatcc cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatatc
     4141 tcatcgcgca acgtcgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt
     4201 tgagtggaag cccaaatcat gaaaatcaaa tgctgcggat caatcggcgc gagtttgccg
     4261 cttttgaccc aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc
     4321 gtcagttcat ccattaacag cggcgcgcct gccagcatct ccatacagaa caggcgcgaa
     4381 gcctgcggat aatcgcgtga gacttccagc ttcagacgga tgtactcttt gatcgccgcc
     4441 agcggggcga aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc
     4501 cgcagcacgg caatatacag cgcctctttt gacgggaagt aatacagcag attggttttt
     4561 gaaacacccg ccaactctgc gatctgctcc agccttgtgc cgtgaaaacc gaattgtgaa
     4621 aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt
     4681 ttacccgttg ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggcaac
     4741 aggatagcaa aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acagatgcat
     4801 gttttatgtg caactgtttt gaccgtttag tccacttttt accagatatt ttagacaaca
     4861 caatgttaac acattaataa cattcacttt ttaaaaactg gcatccgctt tgcaaacaag
     4921 ccattacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg
     4981 agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg
     5041 gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgt
     5101 gcaaaaagcg gagcactacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt
     5161 tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcggggct
     5221 ggcggccgtg acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc
     5281 ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt
     5341 caacctcgtg actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga
     5401 tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct
     5461 gtggggcacg gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt
     5521 cagtccgcaa ccgagtgtcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat
     5581 ggcgttctcc gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacgcc
     5641 cacggctttc gccccgaccg ctgcgcggat gaaacaggcc gcagagcaaa ccgggcgcga
     5701 cgttggttct tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc
     5761 caaatgggaa cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca
     5821 aagtcagaaa gatacccgct ccggtactga taccaacgtc cgtcagatgg ccgatcccac
     5881 ttcggcggta aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat
     5941 gttagatgaa gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttgacct tcgatgattt
     6001 tctgtcggga atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca
     6061 tctacctgcg ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc
     6121 attaccttcg atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa cgcttatgcc
     6181 acgccaggcg gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt
     6241 gccaacattc aaaccgccgt gaccgcagcg cgagcggcag ggatgctgat catctggttt
     6301 caaaatggct gggatgaaca gtatgtcgaa gctggcggcc ccggctcacc gaattttcat
     6361 aaatcgaacg ccctgaaaac catgcgtaag cagccgcagc tgcaggggaa attgctggcg
     6421 aaaggctcct gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg
     6481 ctgccgaagc cgcgctacag cggtttcttc aatacaccgc tggacagcat tttgcgcagc
     6541 cgtggaatac gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg
     6601 ctacgcgatg gcttttttct ggagtatttc ggcgtggtgc tggaagacgc aacacaccag
     6661 gcggggccgg aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg
     6721 gtcagcgacg tcgaaacgtt ctgcgacgcg ctttctccca catcctttgc tcgtatcgct
     6781 taaggagttt aacgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg
     6841 cccccttcgt tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt
     6901 ttgatcaaca taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc
     6961 tggaaactat ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca
     7021 acagcatctt tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt
     7081 tttttccggg tgataaaccg gcgcgattct gcattcagtg cggactggta aaacctgacg
     7141 cgctggtgga aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca
     7201 ctctcacctc ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttgggggt
     7261 agcggcagtt actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt
     7321 tacgaccagc gcggcaccgg caataatccc gacacgctgg cagaagatta cagtatcgcc
     7381 cagatggcag cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcagtg
     7441 gtcggccatg cgctcggtgc gctggtggga atgcagctgg cgctggatta tcccgcgtcg
     7501 gtaactgtgc tgatcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt
     7561 tttcaggttc gcgaacgatt actgtatagc ggcggcgcgc aggcatgggt ggaagcgcag
     7621 ccgttgttcc tctatcccgc cgactggatg gcggcccgcg cacctcgcct tgaggcagaa
     7681 gacgcgctgg cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc
     7741 ctcaaacgcg ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc
     7801 tgcgccagtg atgatctgct ggtgccatca gcatgttcca gtgagcttca tgccgccctg
     7861 cccgatagcc agaaaatggt gatgcgctat ggcggacacg cctgcaacgt gaccgacccc
     7921 gaaacgttta atgctctttt actcaacggg cttgccagcc tgttacatca ccgcgaagcc
     7981 gccctgtaag gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc
     8041 accgatgccc gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg
     8101 gagctttatg ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc
     8161 gtgtttatcc gcactgtaga aggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat
     8221 ctacaaaaaa ccctgaccgc gcccgtcacc gctatcgttg cctgggacag tgaattttat
     8281 gaacggttac cactactgtt tccccacggt gatgcccgca gttggtttac ctccagccca
     8341 caacttgccg aagaaacagc gtttcgcaac agctccatgc aggcggccta tctgatcgtc
     8401 gcttgccgtg cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaatacgtt
     8461 gacgacgctt tttttgcagg cagcacgctg aagagcaatc tgctgattaa tatcggctat
     8521 ggcgatagca gcaaacttta tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg
     8581 ctgttgtaag gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc
     8641 ctgtatgggc gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt
     8701 caccgccagc gccgtctgca gcgtgaccga tacgccgccc acattactgg tgtgcctgaa
     8761 tcgtggggcg tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact
     8821 gagcgccggg caggagccgc tttcaaatct ttttggcggc aaaacgccca tggaacaccg
     8881 ctttgccgcc gcccgctggc agacgggtgt gaccggatgc ccgcaactgg aagaggctct
     8941 ggtttcgttt gattgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt
     9001 ttgcgccatc gaagcgattc atcgtcacgc cacaccctac gggctggtgt ggttcgatcg
     9061 cagttatcac gcgctgatgc gccctgcttg ttaacctcct aaaggagaca gctgatggca
     9121 atgttcggtt ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg
     9181 ccgtatgaac gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg
     9241 atgtttggcg cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt
     9301 ttaatgtcgg ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt
     9361 tatctcggtt ccagcgcggc ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt
     9421 cagggcatta acccgaatat cagcattgcg ctgggcggga ttatcgcctg tgggctggtt
     9481 tataccgtca ttggcctggt ggtgatgaaa attggtacac gctggattga acgactaatg
     9541 ccgccggtgg tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca
     9601 gtgaaaagtg tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt
     9661 attgggctgg tggcggtgtt tacccgtggc atgatccagc ggttattgat tctcgtagga
     9721 ttaattgtcg cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc
     9781 gtcgatttta ctctcgtcag ccatgctgcc tggttcggtt tgccacattt ttccactcct
     9841 gcttttaatg gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa
     9901 aacttaggtc atctcaaggc agtcgccggg atgaccggac gcaatatgga cccgtatatg
     9961 gggcgggcat tcgtaggcga tggtctggca acgatgcttt ccggctctgt cggcggtagc
    10021 ggcgtgacca cctatgctga aaatatcggt gtgatggccg tgaccaaagt ctactcgacg
    10081 ctggtgtttg ttgctgctgc cgtcatcgcc atgctgttgg gcttttcgcc gaaatttggc
    10141 gcgctgatcc ataccattcc tgcaccggtg attggtggtg cttcaattgt tgtgttcggg
    10201 ctgattgccg tcgcaggcgc aaggatctgg gtacaaaacc gtgtcgattt aagccagaac
    10261 ggtaatttga ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg
    10321 ctgggcggtt ttacgttggg agggatcggt acagcaacct ttggcgcgat tttactcaat
    10381 gcgttgttaa gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca
    10441 taaacagaaa accgccgggg taaccggcgg tgatgacttt ttgacgagct tcagacaaaa
    10501 atgcattcgt cactgattag tccggctagt tgtcactttt accgtgactg ctcttgccac
    10561 cttttttacc tgcttcagat gcgcgctgcg ggtcattctt gaaattcccc ccgctgtgct
    10621 gtccaccttt tttacctgct tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac
    10681 cgcctcgatg gtttgccatt actgacctcc gtctgggtat agacatcata ttgcatagaa
    10741 ttaacgcggc gtatggcggc tgccatattt tcgctacgtg ggactaagct tagtgaactg
    10801 acgcgggcag acccgcgttt ggtaatatcc tgcaacagct tattagcgag aatagatatc
    10861 atctccaata attatccata agccgctctt atgattaaaa aaacgcgatg agataaaaat
    10921 gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt
    10981 gtgaaatttg cactctgaaa aggacgtctt atctttaaat aagtggtagc gaatcgctac
    11041 ggaatagaga taacacgagg agtggttaga aatggctaaa gttctggtgc tttattattc
    11101 catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa gcaaagtcga
    11161 tggcgcagaa gttgtcgtta agcgcgtacc ggaaaccatg ccgccgcaat tatttgaaaa
    11221 agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga
    11281 cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt
    11341 cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt
    11401 ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa cctggacgac
    11461 ccttgcgcat cacggcatgg taattgtccc cattggctac gcagcgcagg aattatttga
    11521 cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg
    11581 ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc
    11641 aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa
    11701 gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata
    11761 gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa
    11821 gaaggtagcg atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt
    11881 tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc acttctcgcg
    11941 ggggagtttt ctgttatttc accgcctcat tcaacacgct atcaaactta tccattgggc
    12001 agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg
    12061 cctgcagggt taacgcatcg gcattacgta actgttccgc actctggtac acatattcaa
    12121 ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc
    12181 caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta
    12241 acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt gcgctggtgc
    12301 gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca
    12361 cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac aacaccttcc
    12421 actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtaat
    12481 attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacaccag
    12541 gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa cactgttgtt
    12601 tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagt tggtagctgt
    12661 cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt tcactgaatg
    12721 cggcggaatc atcggtgatc accgggttaa aggttgggtc catagttccc attttttcct
    12781 ggtgatgcac aggaatatca caccccggaa atgcgccggt aataaagaac tgtgcggtcg
    12841 cgacagtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc gggcattccc
    12901 ccgattttac catcccctgc tctgccagcc attcacgcat gtaatggccc atatacactt
    12961 cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct ggccatttat
    13021 tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc
    13081 ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcgt
    13141 ttgaagcgat taaaactatc cctgccacag ctgcggcgat tagcgttttg ttcattcctg
    13201 gcacctcttt tgttatcaat cactaaagag tgtgacagaa atatgacatt tttccgggaa
    13261 caggctcgca aaacagcttg ttgagcggct gtttttatat tcatctttaa aaacaggtaa
    13321 ggtttgatgc aagctatgtc tgcttatccc ctgcctcgcg tacaccgctg atgattac
//
LOCUS       DAFAVF010000114        13344 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFAVF010000114 DAFAVF010000000
VERSION     DAFAVF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.114"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(24..188)
                     /locus_tag="LGK42_003235"
     CDS             complement(24..188)
                     /locus_tag="LGK42_003235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659458.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            297..791
                     /gene="napF"
                     /locus_tag="LGK42_003236"
     CDS             297..791
                     /gene="napF"
                     /locus_tag="LGK42_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBK2659459.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            781..1044
                     /gene="napD"
                     /locus_tag="LGK42_003237"
     CDS             781..1044
                     /gene="napD"
                     /locus_tag="LGK42_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBK2659460.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1041..3527
                     /gene="napA"
                     /locus_tag="LGK42_003238"
     CDS             1041..3527
                     /gene="napA"
                     /locus_tag="LGK42_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HBK2659461.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3534..4229
                     /gene="napG"
                     /locus_tag="LGK42_003239"
     CDS             3534..4229
                     /gene="napG"
                     /locus_tag="LGK42_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBK2659462.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4216..5079
                     /gene="napH"
                     /locus_tag="LGK42_003240"
     CDS             4216..5079
                     /gene="napH"
                     /locus_tag="LGK42_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBK2659463.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5076..5525
                     /gene="napB"
                     /locus_tag="LGK42_003241"
     CDS             5076..5525
                     /gene="napB"
                     /locus_tag="LGK42_003241"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBK2659464.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5535..6137
                     /gene="napC"
                     /locus_tag="LGK42_003242"
     CDS             5535..6137
                     /gene="napC"
                     /locus_tag="LGK42_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBK2659465.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6156..6773
                     /gene="ccmA"
                     /locus_tag="LGK42_003243"
     CDS             6156..6773
                     /gene="ccmA"
                     /locus_tag="LGK42_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBK2659466.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6770..7432
                     /gene="ccmB"
                     /locus_tag="LGK42_003244"
     CDS             6770..7432
                     /gene="ccmB"
                     /locus_tag="LGK42_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBK2659467.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7474..8211
                     /gene="ccmC"
                     /locus_tag="LGK42_003245"
     CDS             7474..8211
                     /gene="ccmC"
                     /locus_tag="LGK42_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBK2659468.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8208..8417
                     /gene="ccmD"
                     /locus_tag="LGK42_003246"
     CDS             8208..8417
                     /gene="ccmD"
                     /locus_tag="LGK42_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBK2659469.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8414..8893
                     /gene="ccmE"
                     /locus_tag="LGK42_003247"
     CDS             8414..8893
                     /gene="ccmE"
                     /locus_tag="LGK42_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBK2659470.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8890..10833
                     /gene="ccmF"
                     /locus_tag="LGK42_003248"
     CDS             8890..10833
                     /gene="ccmF"
                     /locus_tag="LGK42_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBK2659471.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10830..11387
                     /gene="dsbE"
                     /locus_tag="LGK42_003249"
     CDS             10830..11387
                     /gene="dsbE"
                     /locus_tag="LGK42_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBK2659472.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11384..12436
                     /gene="ccmH"
                     /locus_tag="LGK42_003250"
     CDS             11384..12436
                     /gene="ccmH"
                     /locus_tag="LGK42_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBK2659473.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSLQESK"
     gene            complement(12471..13118)
                     /gene="narP"
                     /locus_tag="LGK42_003251"
     CDS             complement(12471..13118)
                     /gene="narP"
                     /locus_tag="LGK42_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBK2659474.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         2822 a         3393 c         3931 g         3198 t
ORIGIN      
        1 caattttacc tgctctttta gtgttaaatt ctgatgaggg agcaaaccag cagaaatatc
       61 tctggtgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc
      121 cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg
      181 gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta
      241 tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga
      301 agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta
      361 tccgtccacc ctggtccggt gatgaatctc attttctgac ccattgcacc cgttgcgacg
      421 cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg
      481 ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat
      541 cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt ggggacgcgt
      601 gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa
      661 ttatctttcg cccgacgctc tccggaatct accagccgca acttaatagc caactttgca
      721 acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc
      781 atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca
      841 gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg
      901 agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt
      961 gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa
     1021 gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg
     1081 ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg
     1141 gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg
     1201 gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac
     1261 cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta
     1321 aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg
     1381 aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg
     1441 ccctgaaaga aaaaggaccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct
     1501 gggaaggtta tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac aacatcgacc
     1561 cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg
     1621 atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg
     1681 ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaaccgt cgtctctcta
     1741 accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg
     1801 ataacggcat catctttacg ccgcaatctg acctggtgat cctgaactac atcgccaact
     1861 atatcattca aaacaatgcg ataaatcagg acttcttcag caagcacgtt aacctgcgca
     1921 aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga
     1981 agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg
     2041 ctgagtatac gctggaaaaa actgccgaaa tgaccggcgt gccgaaagac cagttagaac
     2101 aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct
     2161 tcaaccagca tactcgtggc gtgtgggcca acaacctggt ctacaacctg cacctgctga
     2221 ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttccgcgt
     2281 gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtaa
     2341 ctaacgagaa acatcgtgat atctgcgaga agaagtggaa tatcccgagc ggcaccattc
     2401 cggcgaaaat cggtctgcat gcggtagcac aagaccgtgc gctgaaagac ggcaagctga
     2461 atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc aatgaagagc
     2521 gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag
     2581 tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggcg
     2641 cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag gcgccaggcg
     2701 aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag
     2761 tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag
     2821 ttctgtatgc cacccccgaa gtgagcaaat accctgtatc cgaactggcg gaagatcagc
     2881 tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg
     2941 catggtttgg tcgcggtcac ggtcacgatc tggcaccgtt cgatgactac cacaaagcgc
     3001 gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta
     3061 acgacccgta cgtgaaagcg ggcgaaggct acaagttcta cggtaaaccg gatggcaaag
     3121 cggtgatctt cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc
     3181 tgtggctctc taccggacgc gttctggagc actggcacac cggcagtatg actcgccgtg
     3241 taccagaact gcaccgcgcc ttcccggaag cggtcctgtt tattcacccg ctggatgcga
     3301 aagcgcgcga tctgcgtcgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga
     3361 tctcgattgt cgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt
     3421 tcttcgacgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat ccgctctcga
     3481 aagagacgga cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc
     3541 ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt cgcacagcag
     3601 gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg
     3661 gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc
     3721 gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggctt
     3781 ctggcctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg
     3841 aggacattcc gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag attgaatcga
     3901 tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc
     3961 aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc
     4021 tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca
     4081 gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa caaccggcaa
     4141 tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggct
     4201 ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa
     4261 aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg
     4321 ctggggatgt ttttgagcgg tccgtggttt ggtgtgtgga tcttacacgg taactacagc
     4381 agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt
     4441 gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat caccgtgctc
     4501 tacgccctgg cgggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc
     4561 gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc
     4621 cacattcgct acgtgctgtt ggtggtcatt ctggtgggat cagccttaac tggcacgctc
     4681 atttgggaat ggatcaaccc cgtttctctg atggggcgca gcctggtaat gggcttcggc
     4741 agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc
     4801 tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg
     4861 attaccgttg cggccaccga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt
     4921 tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc
     4981 accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt
     5041 acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag
     5101 cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta
     5161 atggcgtcga ttttagccag tcgccggaag tatccgggac acaggaaggg gccattcgga
     5221 tgccaaaaga gcaggaacgg atgccgctga actatgtgaa tcagccgcca atgatcccgc
     5281 atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg
     5341 tcgaaagtta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg
     5401 acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac
     5461 cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga
     5521 aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga
     5581 aatggtggcg tacccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgttg
     5641 gcggcatcgt cttctggggt ggctttaaca ccgggatgga aaaagccaat accgaagagt
     5701 tctgcattag ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg gattccgtgc
     5761 actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt
     5821 ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg
     5881 gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca cagaatgagt
     5941 ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata
     6001 tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg
     6061 ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag
     6121 tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt
     6181 gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt
     6241 gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg
     6301 ggttgtctcg ccctgacgca ggcgaggttc tctggcaagg gcagcccttg catcaggtac
     6361 gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc
     6421 tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg
     6481 aagccctggc gcaggccggg cttgccggat tcgaagatat tcctgtaaat cagctctcgg
     6541 ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga
     6601 tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta
     6661 tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg
     6721 ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca tgatgttctg
     6781 gcgcattttc cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc
     6841 gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca
     6901 actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct
     6961 ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt
     7021 gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac
     7081 cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa tggatgttta
     7141 tggctggcaa gtgatggcgc tgacgcttct gctgggaacg cctacgcttg gctttctcgg
     7201 tgcaccgggt gtggcgttga cagtgggact taagcgcggt ggtgtgctgc tcagcatact
     7261 ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc
     7321 catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc
     7381 gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat
     7441 tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg
     7501 atccctccac ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt
     7561 gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag
     7621 cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc
     7681 atttatgcat caatggcggt ggcggctttt attggccttg tctggcagat gaaaatggcc
     7741 aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt
     7801 accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg
     7861 acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc
     7921 gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat
     7981 ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg
     8041 atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc
     8101 ctgctgctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa
     8161 aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat
     8221 ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg
     8281 tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg cagcatcgcg
     8341 caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac
     8401 agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt
     8461 ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct
     8521 cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt
     8581 cgggcagcgt ctgcgcgtcg gcgggatggt gatgccgggt agcgtgcagc gcgatcccaa
     8641 ttcgctaaaa gtaactttca ccatttacga cgctgaaggt tcagtagatg tctcttacga
     8701 aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga
     8761 aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc
     8821 gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga
     8881 cccagcatca tgatgccgga aattggaaac gggctgctgt gcctggcgtt agggattgcg
     8941 ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg catgatggcg
     9001 tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg
     9061 gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa ctccaatacc
     9121 cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctactg
     9181 ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt
     9241 ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg
     9301 ctgttcattc tctttacctc taacccattc tcacgcacgc tgccgaactt cccgattgaa
     9361 gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc gcctctgctt
     9421 tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc tttgttgtgc
     9481 gggcgtctgg acagcactta tgcgcgtttt actcgtccgt ggacgctggc ggcgtggatc
     9541 ttcttgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc
     9601 ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact
     9661 gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta
     9721 ctgctggcaa tcagtgcctt ctcgttgtgt ctgctgggga ctttcctggt gcgttccggc
     9781 gtgctggtat cggtacacgc gtttgcctct gatccggcac gcggtatgtt tatcctcgcc
     9841 tttatggtac tggtgattgg tggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt
     9901 cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt
     9961 ttgctggtcg ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag
    10021 caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg
    10081 ctgatggtgc cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat
    10141 cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg
    10201 ctgctgctgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggcctggca
    10261 atggcctgct ggattgctgt gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc
    10321 acgaaaacca ccttcagtta ttggggaatg gtggcggctc acctggggct ggcagtgaca
    10381 attgttggta ttgcctttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc
    10441 ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact
    10501 ggcccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgcgatgg caagccggaa
    10561 acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg
    10621 gcaattgacg gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac
    10681 ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg
    10741 ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg
    10801 agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta attccgttga
    10861 ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg
    10921 atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag tttcgtctcg
    10981 aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag ggcaaaccag
    11041 tgctgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga
    11101 atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac gatcgccaga
    11161 aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg
    11221 acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaaccttc cttattgacg
    11281 gcaacggtat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag
    11341 agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg
    11401 cgtgctgatg ctgatgatct ccggctcagc actggcgacc atcgacgtgt tgcagtttaa
    11461 agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg
    11521 ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc gtcagaaagt
    11581 gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg
    11641 ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt
    11701 gcttccggta gtggctattg gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg
    11761 ggtacgcgta gtgccggaag cgtttcctga acaaagcgtg caggaaggta agcgtgccgg
    11821 atatattgtt tatctgccgg gtattgtggt ggcgttaatt gtggctggcg tcagctacta
    11881 ccagactggc aattatcagc aggtgaaaat ctggcagcag gccacggcac aggctccggc
    11941 gttgctggac agggcgctgg atccgaaagc cgatccgctc aacgaagaag agatgtcgcg
    12001 tcttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag aaggctggat
    12061 tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg atgcatacgc
    12121 tactgcgtat cgcctcgatc cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt
    12181 gacacgttca tctgatccca acgacaaccg cctcggcggc gaactgctgc gccagttggt
    12241 gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg cctttgagca
    12301 gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa
    12361 cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgct
    12421 gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc
    12481 ccgcgttgtt gcaggaacag aatggtggcc gccacgcgtg agcggacatt gagtttacgc
    12541 agcagattgc gaatatgtac ttttactgtc tgctcggaaa tattcaatac cgaggcaatc
    12601 tgtttatttg acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc
    12661 acgctgaagg gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg
    12721 cgttcgctaa agactttgct gcctttcgct ccggcacgaa tcgcttccag caatacttcc
    12781 gggtcgctgt ctttcaacag ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg
    12841 ctggaggcat cggatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag
    12901 gcattgaggg tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg
    12961 atatccagtc tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact
    13021 tcaaagccag gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca
    13081 tccacaatca tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt
    13141 agaatcatta tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca
    13201 ttttatgtat ggacatgtag tgaaaactac aaccttgtcg ttcgctttgg catcattttc
    13261 attataaaga cggaattttg catcagcttc tcatcaagaa aaacaactaa aaaatcaatc
    13321 acaatacttg tgcatttctt taaa
//
LOCUS       DAFAVF010000115        13229 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFAVF010000115 DAFAVF010000000
VERSION     DAFAVF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.115"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            557..1246
                     /gene="mutH"
                     /locus_tag="LGK42_003252"
     CDS             557..1246
                     /gene="mutH"
                     /locus_tag="LGK42_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBK2659475.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1315..2028
                     /locus_tag="LGK42_003253"
     CDS             1315..2028
                     /locus_tag="LGK42_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBK2659476.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2166..2384
                     /gene="ygdR"
                     /locus_tag="LGK42_003254"
     CDS             2166..2384
                     /gene="ygdR"
                     /locus_tag="LGK42_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBK2659477.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2492..3532
                     /locus_tag="LGK42_003255"
     CDS             2492..3532
                     /locus_tag="LGK42_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBK2659478.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3564..4757)
                     /gene="lplT"
                     /locus_tag="LGK42_003256"
     CDS             complement(3564..4757)
                     /gene="lplT"
                     /locus_tag="LGK42_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBK2659479.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4750..6909)
                     /gene="aas"
                     /locus_tag="LGK42_003257"
     CDS             complement(4750..6909)
                     /gene="aas"
                     /locus_tag="LGK42_003257"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBK2659480.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7495..8526
                     /gene="galR"
                     /locus_tag="LGK42_003258"
     CDS             7495..8526
                     /gene="galR"
                     /locus_tag="LGK42_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBK2659481.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8533..9795)
                     /gene="lysA"
                     /locus_tag="LGK42_003259"
     CDS             complement(8533..9795)
                     /gene="lysA"
                     /locus_tag="LGK42_003259"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK2659482.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9917..10852
                     /gene="lysR"
                     /locus_tag="LGK42_003260"
     CDS             9917..10852
                     /gene="lysR"
                     /locus_tag="LGK42_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBK2659483.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10839..11531)
                     /gene="ygeA"
                     /locus_tag="LGK42_003261"
     CDS             complement(10839..11531)
                     /gene="ygeA"
                     /locus_tag="LGK42_003261"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBK2659484.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11660..13078)
                     /gene="araE"
                     /locus_tag="LGK42_003262"
     CDS             complement(11660..13078)
                     /gene="araE"
                     /locus_tag="LGK42_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBK2659485.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3376 a         3528 c         3244 g         3081 t
ORIGIN      
        1 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
       61 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
      121 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
      181 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
      241 ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcatcg
      301 cacgtcaagg ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct
      361 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
      421 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag ggaattgaac
      481 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
      541 atcaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
      601 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
      661 tgccgggctt gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
      721 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ccgctctggg
      781 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
      841 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
      901 caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
      961 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
     1021 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
     1081 tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga
     1141 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
     1201 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
     1261 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
     1321 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
     1381 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca
     1441 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
     1501 ctgctggcat caatcgcctg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
     1561 caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
     1621 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
     1681 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt tagcctcgac
     1741 tcggtgatta cggctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg
     1801 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
     1861 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
     1921 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
     1981 gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc
     2041 tctcctgcgg gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt
     2101 caagatatat aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata
     2161 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
     2221 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
     2281 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
     2341 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
     2401 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
     2461 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
     2521 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
     2581 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
     2641 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
     2701 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
     2761 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
     2821 ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca
     2881 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
     2941 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
     3001 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
     3061 gcatctagcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
     3121 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
     3181 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
     3241 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
     3301 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctgg accctgctca
     3361 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     3421 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     3481 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     3541 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     3601 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctatcatt
     3661 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     3721 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     3781 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     3841 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     3901 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     3961 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     4021 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     4081 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     4141 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     4201 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     4261 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     4321 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     4381 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     4441 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     4501 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     4561 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     4621 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     4681 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     4741 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     4801 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     4861 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     4921 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     4981 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     5041 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     5101 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     5161 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     5221 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     5281 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     5341 gttgatagaa acgacaggcg cgcactcggt tacaccatag ccttcaagga tgcgcaggcc
     5401 aaatttatcc tgccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac
     5461 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     5521 cgaggtaccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatgcggta
     5581 atgcagcggg cttggataaa ggaacacttc tgcacctgta agcagtggcg taaacaggcc
     5641 taccgtcagc ccaaaggagt gaaacagcgg taatgccgac ataaagcgat cgttggtggt
     5701 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     5761 gacgcctttt ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     5821 ctgctgtttt acctgcgcca ggcgcggcat cagcaaatga gcgaagatcc atactttgtc
     5881 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     5941 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     6001 gatttcagcc gccgtaatag cactggtcag cccttttacc ccagcagtgt agttcatcat
     6061 tgcgggaatg cggcgacgcg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     6121 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     6181 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     6241 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagagtttc
     6301 acgcgggcgc accgccatac gcgcttccat cattatttga tgcagcattt caccagcgat
     6361 tttgcgacgg tcacgggcac gcggcgcatc cggcatcgcc acctgcgttg gtggcaaaat
     6421 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     6481 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     6541 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgta gtggtgatgc gtccttcagg
     6601 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tagccatagg
     6661 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     6721 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     6781 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     6841 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     6901 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     6961 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     7021 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     7081 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     7141 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcca
     7201 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     7261 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg
     7321 gtcaatcgca acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     7381 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg
     7441 ggcgttttcc gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg
     7501 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     7561 aataattcac ctaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct
     7621 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     7681 ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     7741 caggtggctt atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa
     7801 aaagagcgtc aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     7861 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     7921 ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac
     7981 ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg
     8041 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     8101 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc
     8161 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     8221 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     8281 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgc
     8341 ccgcgcctga ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg
     8401 gctttggcgc tggcggataa tcgccctctc ccggaaatta ctaatgtctt tagtccgacg
     8461 ctggtacgtc gccattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     8521 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     8581 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     8641 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     8701 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     8761 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     8821 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     8881 cagatcgttg aacccagcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     8941 ccgcacctga gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc
     9001 cagtttcaca gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc
     9061 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc
     9121 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc
     9181 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     9241 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     9301 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     9361 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     9421 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     9481 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     9541 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     9601 cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     9661 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     9721 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     9781 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     9841 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga
     9901 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     9961 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
    10021 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
    10081 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
    10141 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
    10201 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
    10261 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
    10321 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
    10381 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
    10441 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
    10501 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
    10561 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
    10621 ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg
    10681 tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
    10741 gcaccgcccg tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc
    10801 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
    10861 gcatcctcgg catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc
    10921 accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg
    10981 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
    11041 tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg
    11101 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
    11161 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
    11221 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
    11281 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
    11341 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
    11401 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
    11461 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
    11521 attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag
    11581 acaaacgcct caacggccag gtgcccgatc tctattaacg aaaaaagggc cggatgtaca
    11641 gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt
    11701 tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc
    11761 ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat
    11821 caagcagtgt caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg
    11881 aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca
    11941 ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga
    12001 gccaggacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca
    12061 ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt
    12121 tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag
    12181 tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcat
    12241 agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa
    12301 acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgcct tgttttaact
    12361 tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca
    12421 tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat
    12481 ttggcaggaa gaccaccaga ataatcagca ggactgctgg taaagcaaga acccccaaca
    12541 ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc
    12601 cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca
    12661 tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca
    12721 cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta
    12781 caaacaggat ggcccccgcc atcaggctgt atttacgacc caggcggaac gacagccaac
    12841 cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca
    12901 aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga
    12961 tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac
    13021 gccgcgtatc ccgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt
    13081 tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaactc acgcaggtgt
    13141 caggtcggaa acagcataaa tatggattaa attgctacga catgtcgtta tgtgatggat
    13201 attccaattt tcaaattaag ttgaattat
//
LOCUS       DAFAVF010000116        13100 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFAVF010000116 DAFAVF010000000
VERSION     DAFAVF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.116"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            3..1949
                     /gene="yegI"
                     /locus_tag="LGK42_003263"
     CDS             3..1949
                     /gene="yegI"
                     /locus_tag="LGK42_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBK2659486.1"
                     /translation="MKTNIKVFTSTGELTTLGRELGKGGEGAVYDIEEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGV
                     IYFIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRRE
                     YIILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFN
                     RAKMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(1961..3313)
                     /gene="yegD"
                     /locus_tag="LGK42_003264"
     CDS             complement(1961..3313)
                     /gene="yegD"
                     /locus_tag="LGK42_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBK2659487.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3447..4304
                     /gene="alkA"
                     /locus_tag="LGK42_003265"
     CDS             3447..4304
                     /gene="alkA"
                     /locus_tag="LGK42_003265"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBK2659488.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(4343..7660)
                     /locus_tag="LGK42_003266"
     CDS             complement(4343..7660)
                     /locus_tag="LGK42_003266"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBK2659489.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSALEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISISLPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            7978..8619
                     /gene="udk"
                     /locus_tag="LGK42_003267"
     CDS             7978..8619
                     /gene="udk"
                     /locus_tag="LGK42_003267"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK2659490.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8711..9292
                     /gene="dcd"
                     /locus_tag="LGK42_003268"
     CDS             8711..9292
                     /gene="dcd"
                     /locus_tag="LGK42_003268"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBK2659491.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9314..11167
                     /gene="asmA"
                     /locus_tag="LGK42_003269"
     CDS             9314..11167
                     /gene="asmA"
                     /locus_tag="LGK42_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBK2659492.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQLMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11460..13043)
                     /locus_tag="LGK42_003270"
     CDS             complement(11460..13043)
                     /locus_tag="LGK42_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBK2659493.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         3156 a         3422 c         3479 g         3043 t
ORIGIN      
        1 ttatgaaaac aaatataaaa gtatttacat cgacaggtga attgaccact cttggccgtg
       61 aactgggcaa aggtggtgaa ggcgcggttt atgatatcga ggagtttgtc gatagcgtcg
      121 ccaagattta tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg
      181 cgacagctga cgcgcaattg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg
      241 gacgaggcgg aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc
      301 atatgatcta tagcccggca catcgtcgtc aacgttaccc tcattgtgcg tgggattttc
      361 tactctatgt tgcgcgcaat attgcttcat cttttgctac ggttcacgag cacgggcacg
      421 tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg cgacagcaaa gtggtactga
      481 ttgatagcga ctcctttcag attaacgcca atggcacact gcatttatgc gaagtcggcg
      541 tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc atttgtcggc tttgaacgca
      601 cagaaaatca cgataatttt ggccttgcgt tgctgatttt tcacgtcttg tttggtggcc
      661 ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg taatgcgctg gagacggata
      721 ttacccattt ccgttatgcc tacgcgtcag ataatcagcg acgtggttta aaaccgccgc
      781 cacgatcgat tccgctatcg atgttaccga gtgatgttga agccatgttt cagcaggcat
      841 tcacggaaag tggcgtggca accgggcgtc cgacggctaa agcgtgggta gcggcacttg
      901 attctctgcg ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt tatcccggtc
      961 atttggctga ctgcccatgg tgtgcgctgg ataatcaagg tgttatctat tttattgatc
     1021 tcggcgaaga ggtcattacc accggcgggg attttgtgct ggcaaaagtc tgggcgatgg
     1081 tgatggcgtc agtagcaccg ccagcattgc aactgccatt acccgatcat ttccaaccga
     1141 ctggcaggcc gcttccttta ggcctgttac ggcgcgaata catcattctg cttgagatcg
     1201 cactgtcagc attatcgctg ctgctttgcg gccttcaggc agaaccgcgt tatattattt
     1261 tggttcctgt gctggcggct atctggatta ttggcagtct gacaagcaaa gcgtacaaag
     1321 cagaagttca gcaacgccgt gaggcattta atcgtgcgaa aatggactat gaccatttag
     1381 tccgccagat ccagcaggta ggcggcctgg aaggttttat cgccaaacgg acgatgctcg
     1441 aaaaaatgaa ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggcagcac
     1501 ttcatgacac tgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg
     1561 ttgcctctat tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg
     1621 aaacagccgc agatgttacc cgtcgtggcg ttaagcaagt taaagggttt ggcgaccatc
     1681 tgacccaggc ggttatcgac tggaaagcga gctgtgaacg ccgttttgtg ttcaggccga
     1741 atgaagcgat aacgccagca gacagacaag cggtaatggc gaaaatgacc gccaaacgac
     1801 atcggctgga atcgacgttg actgtcggcg cgacagagtt gcagcgattc cgccttcatg
     1861 ctccagcacg gaccatgccg ttgatggaac ctttacgtca ggcggcagaa aaactggctc
     1921 aggcgcaggc agatttaagc cgctgctgag ccattttctt ttaacgaaac accacttccg
     1981 cccagcgtgc cagcccggcg gtgacggagc caaagtcatc gccgcctgca atcggaattc
     2041 ccggcaactg ttctgccagc gcttttttaa tcagcggcga acgggcgcta ccgccggtca
     2101 ggtagataac gtccggtttt tcctgggcgt tatccagggc cagttgcacc tgttccagaa
     2161 tccgcgccag tggctggctg agggcgcttt ccagcccttg ctggctaatc agcgtagcca
     2221 gttcatcgct gataaacggc agtgaggcgc gggtttccgc tacgcttgaa agagcaattt
     2281 tgcactcttc tgcgctacgc accaggcgat agcttaaacg ctgacgccag actttctgta
     2341 gcagggccac tttttccggt tcgcgggcat cgcgtaccag atcgttaagc agacgaccgt
     2401 tggcactact gtagaaatca ctctgcgcag gtacgtcgtt gatggcaacc gcattccacc
     2461 acggcagaat cggcagggcg atgccttttt cggtttcgcc acccatgccc agtaacggca
     2521 tcaggttttt aaacgccagt gcgatgtcca gatcgttacc gccaatacgg caaccactgt
     2581 gacccagcag gctggcttca cgatcgagac gcgaacgcca ctgcggcccc atcagcagca
     2641 atgaacagtc agtcgtacca ccgccgatat ccaccaccag cacccgtttt tcttcctgca
     2701 aggtggcttc gtaatccagc ccagccgcga ccggctcgta ctggaatacc acgtccctga
     2761 atccggcacg cttcgccgcg cgttccagaa tcccttgcgc ctgggcgttt gcttcatcgc
     2821 cgcccagccc ctggaagttg atcgggcgac caatcaccgc ctgagtaatc gcttctggca
     2881 gctgcgcctg cgcctgctgg cgaatgtgca acatcattgc gcagaccaga tcctcaaaca
     2941 gcgctacctg ctgcggtttt aagccgctgg caccgaggaa cgattttggt gatttcacaa
     3001 accacacttc ttctggatca tcaatgtact gtgccagtga ggaaagaccg aactgcacgc
     3061 ttttcgccgt aacatcgata tcttcttcgc ggttataacg aatcgcccga cgtaacagcg
     3121 cctgcgtttc atcgtcgtct gccggaacat catgatggcg gtacagccat tcacttactg
     3181 cttcacgcgt tggcgcgcaa agcattgaag gcagcagcgt gctgtcgttt tccattttca
     3241 gtaattgcgg tttaccgtca cgcatgaccg ccactgaaca gtttgctgta ccgtaatcaa
     3301 aaccaataaa cacgaattaa tccccatgcc ggtgaagaag gggcgtgact ttagcgaaat
     3361 gttgccgtcg cgacaaccgg aatatgaaag caaagcgtag cgtctgaata acgtttatgc
     3421 tgaaagcgga tgaataagga gatgcgatgt ataccctgaa ctggcagccg ccgtatgact
     3481 ggtcgtggat gttgggattt ctcgccgccc gtgcggtgag tggtgtggaa acggtcgctg
     3541 aggattatta tgcccgtagt ctggcggtgg gcgaatatcg cggcgtggtg actgctattc
     3601 cggatatagc ccgccatact ctgcacataa atttaagtgc aggtttagaa cccgttgccg
     3661 ctgagtgtct ggcgaaaatg agccgcctgt ttgatctgca atgtaacccg cagattgtta
     3721 acggtgcgtt gggcaagtta ggcgcggcgc ggcccggatt gcgtttaccc ggctgtattg
     3781 atgcttttga gcagggtgtg cgggcgattt taggccaact ggtgagcgtg gcgatggcgg
     3841 caaaattgac tgccaaagtt gtacagcttt atggcgaacg gctggatgat tttccggagt
     3901 atatctgctt ccccacgcct cagcggctgg cagcagccga cccgcaggca ttaaaagcgt
     3961 taggcatgcc gttgaaacgg gcagaggcac tgattcatct ggcaaatgcg gcgctggagg
     4021 gcagcttacc aatgacaatt ccgggcgatg tggagcaggc gatgaaaacg ctgcaaactt
     4081 ttccgggtat cgggcgctgg acggcgaatt atttcgcttt gcgtggctgg caggcgaaag
     4141 atgtttttct gccggatgat tatctgatta aacagcgatt tccggggatg acaccggcac
     4201 aaatccgccg ttatgccgag cgctggaagc cctggcgttc ttatgcgctg ttgcatatct
     4261 ggtatacgga aggctggcaa ccagacggaa cggacgaatt gtaggcctga tacgtcgcat
     4321 caggcaatgg gcaaacgctc cctcagttaa tcgcgaaata actgctattc accagcaaat
     4381 ccagcggttg ggcatcggca atcacatcac cataaatcag atcgacgccg atgccagaaa
     4441 gcgtatccat cactaagggt aaaacgactg gcccggcgat ggttttcatc ccgagtcgct
     4501 gagcgtgccc ctgaataatc gtaatcagca tctcatccat caaattaccc tgcacgttgg
     4561 cgcataactc accatcaagt agcaggtaat ctgccatatt cgctttcagc gagttgaaga
     4621 tttgcagatc gcggcccacc tggctgagca ctatccgaca tcccgccagc cgcagttttt
     4681 gcacgctttc tgcgtgatct aaaatcgctt cagccggaat aatcaggtgt aataaccgtg
     4741 gtggtagagg gctattttcc agctgctcaa gcagatcatt caccagcgtg gcgctactca
     4801 aaccggcaac ggaaaggggg agggagatgc ttatgccttt actggcaacc gcttttgcgg
     4861 cctgctggaa aaattcgtgg aatacccggc ggtcaagagc atggctaagc gccggatcgc
     4921 tgaagctacg acgaaatgtt tgttcatcaa taatctcgcc ttcgcaactc cagagcttaa
     4981 gtgaaatcag ccacaaatta cgcgcttccg ggatccgtgg cgaagcgaca ccgtgggcga
     5041 gcatcatcaa ctgattctct ttaatcatcc gccactgttc atcaagcgac atcgccgccc
     5101 gttcgctatg tgcggcagct tgctgcggtt cgtaaaccgt cacccggccc cggccaccat
     5161 ttttggaggc ataacaggcg atatcagcct gcgacatcac ttctgccgcc tgatgattgt
     5221 tgtcatcaat caaggtaatc ccggcactgg cacctacccg atgcacacgg ccttcccata
     5281 taaagtgata gtcattcacg gcactgataa tgcgtgtagc gataaaacgc gcgctttcaa
     5341 cattacagtc tggtagcagc agaccaaatt catccccacc gagtcgcgcc agcacgtcgc
     5401 tggaacgcag catactcagc attaacgacg ccagttcgcg cagcaaagcg tcacccgccg
     5461 catgcccggc gctgtcattc accgctttaa agcgatcaag atcgataaac accagggcat
     5521 gtcgctgatg tgtactgttt accgtttgca gcaggatacg cagttgtttc tcaaaactgg
     5581 cgcggttggc gagatgcgtc agtgcatcat gggaggcgct gtagctcagc tggcgcagca
     5641 ttttgcgtga ttcggtaacg tcctgaatca ccagaaccga accaatattg ctgccgtcca
     5701 gagtacttaa cggcgtaata ctgtaatgca cgtcgtaact gccgccgctc cgacagtgca
     5761 acaccacatc ttgctcgagc gcggaacgtg aggtgtcggc actgtaaatg ttctccatta
     5821 atggtccgtt gtcgccaaaa gtaatatgca acaccgtcag gagcggaaca cctaacgctt
     5881 cttcctgcgt ccagccgctc atcttctccg ccactggatt cataaaggta attttcatcg
     5941 ccatatcaat acagaccacg gcttcaccga tggaatcaag cgtaatgtgc aggcgctctt
     6001 tttcctgaaa caatgcctcg ttaagctgtt tcacttcggt catatccata ttgatgccga
     6061 ggagacgttc gacttcgcct tctttattca gtacccggtt ggcgagggcg cggatatggc
     6121 gaataccgtc ttttacggta atgcgaaatt ccagtttaaa gggcgagcgt gattgcaacg
     6181 aatcacgaat cactttttcg gcatgctggc gatcttccgg cagcacgcac tcgtaccaca
     6241 cctgccagtt cggtttgata tgtggaggaa tttcatacag ctcgaacatc cgcttatccc
     6301 agctaaaaat attcggcttc aactcccact cccagatgcc aatcccgccc gcttcgttag
     6361 ccagagtgat gcgctccatc agttgctgat tgacctgttc ggtgcgtttt agctcgttaa
     6421 tgtcttcaat ctgagcgata aaatagagcg gcgtgccatc cgtgtggcgc accagtgaga
     6481 cggcaagcaa cgcccagaca acatcgccat tgcggttgta gtagcgtttt tccattgaat
     6541 aggtgtttat ttcaccgctt atcagctttt caacctgttg gagatcttta ttgagatcct
     6601 ccggccaggt cagttgctga aaggtgagtc cgcgcagctc ttcctgactg tacccgagaa
     6661 actggcagag cgctttgttg gtttgcagcc attgtccctc ggtgcccact aacgccatac
     6721 cgatagcgga atattccatc gcgttccgaa agtgggtttc gctttcggaa atgtgtttgc
     6781 gttccgcacg aaaggcatac atcaccatgg tcatgatgtt ggcgggcagc aggatcagca
     6841 aaaacggtag ccacggcata tggctcatca ggtacgtacg cggcgtagca agcagggagg
     6901 gatccgcggc catcatcagc gacaccatca tcaccgtggt aaggaagatc aaaaaggctt
     6961 ccattcgtgg cagacgcacg gcgctccaca tcaacagcac aataatgaaa gtaaaaggcc
     7021 acggcagata cagcatcgaa agccagctta acgtcagtgt gatggctaac gtgagcagcg
     7081 actcaaaaag caaccgtggg ttgcgatggc gcagcagata gtgtggttta aataacaatc
     7141 ccagcggcac cagtgccaga gcgccgatgg attctgacag tacccatatc aaaaatgccc
     7201 tgagaggatc gtctccgggc gtcagcagga caaccagaac accccccaac agaggcggaa
     7261 caatggcgct gccgagtgcc agacgcagcc agtcagccag attttgcaag gggttatacc
     7321 acggcagcaa tttacgtagc agcactgccc cgaccacggc ttcaacaata ttgatggtcg
     7381 tccaggtcat gttcagcgag ctggtggaaa aaagcaggat ggatgcggcg atatttccca
     7441 gcgaacagct cagcgcaatt cccggccaca tgcgcccggc atggcgataa aacgccacca
     7501 tcatgatgga cgtcgggaac cacagtgggg cgagttgggt gccaaactgc gaaagctcaa
     7561 gcgagaaaag ggtaaagata aacgagacta aacctaaact gaccaggtga agcagcgggt
     7621 ggggcagggc aattaataca tgctgtgatt gtttgctcat taccgcttta tccgcaagac
     7681 ctggtcgcca gtattcgttt ctgtgtttat gctagtacaa aagttttaca ttttatatgc
     7741 gaattgctca taaagtgacg taaaggcgga taatttgcgc aactgcgtat aacatttttt
     7801 accttacata aaactgatca acgtaatttg cccccgggaa ttctttgtca acaattaact
     7861 ctgagcgcgc ggcaacgcta ttcgactggt atcagacgga tgaaatccct ataattgccg
     7921 cgtttggcgc ttcgtcgccc ccttcctaac atccaggtta atcaggtcgc taaatatatg
     7981 actgatcagt ctcatcagtg cgtcattatc ggtatcgctg gcgcatcggc ttccggcaag
     8041 agtcttattg ccagtaccct ttatcgtgaa ttgcgtgagc aagtcggtga tgaacacatc
     8101 ggcgtaattc ccgaagactg ctattacaaa gatcaaagcc atctgtcgat ggaagaacgc
     8161 gttaagacca actacgacca tcccagcgcg atggatcaca gtctgctgct tgagcattta
     8221 caagcgttga aacgcggctc ggcaattgac ctgccggttt acagctatgt tgaacatacg
     8281 cgtatgaaag aaacggtgac ggttgagccg aagaaggtca tcattctcga aggcattttg
     8341 ttgctgacgg atgcgcgttt gcgtgacgaa cttaacttct ccattttcgt tgataccccg
     8401 ctggatatct gcctgatgcg ccgcatcaag cgtgacgtta acgagcgtgg gcgttcaatg
     8461 gattcagtga tggcgcaata tcaaaaaacc gtgcgcccga tgttcctgca attcattgag
     8521 ccttctaaac aatatgcgga cattatcgtg ccgcgcggcg ggaaaaaccg catcgcgatc
     8581 gatatattga aagcgaaaat aagtcagttc tttgaataag cttgataaat tgtgtaccgt
     8641 tcagtgataa cctggtatgc ccttgacgta attggcgtta agggagtgat gcgcgaaagg
     8701 agaaaatgcc atgcgtctgt gtgaccgaga tattgaagcc tggcttgatg aaggccgttt
     8761 gtcgatcaac ccacgtccgc cagtggagcg tattaacggc gcgacggtgg atgtacgcct
     8821 gggcaataaa tttcgtacct tccgtggtca cacggcagcg tttatcgatc tgagcggtcc
     8881 caaagatgaa gtgagcgccg cgcttgaccg cgtgatgagc gatgagatcg ttctcgacga
     8941 gggcgaggcg ttctatcttc acccaggaga gctggcgctg gcggtgacgc tggagtcggt
     9001 gacgctgcca gccgatctgg tgggctggct ggacgggcgt tcctcactgg cgcgtctggg
     9061 gctgatggtg cacgtcaccg cgcaccgcat cgatccgggc tggtctggtt gcattgtgct
     9121 ggagttctac aactccggta agctgccgct ggcgctgcgt ccgggcatgt taattggtgc
     9181 gctgagcttt gagccgcttt ccggcccggc ggcgcgacct tacaaccgcc gtgaagatgc
     9241 gaaatatcgc aaccagcagg gcgcggtagc cagccgaatc gataaagact aattcaggcc
     9301 cattgaggat accatgagac gatttctgac gacgctgatg atactcctgg tcgtgctggt
     9361 ggccgggtta tctgcgttag tgttgctggt gaatccgaat gatttccgcg actatatggt
     9421 caagcaagtt gctgcacgta gcggttatca attgcagctc gacgggccac tgcgttggca
     9481 cgtctggccg cagcttagta tcctctccgg gcgaatgtct ctcaccgccc agggcgcaag
     9541 ccagccactg gttcgcgccg acaacatgcg tctggacgtg gcgcttttac cactactgag
     9601 tcatcaactg agcgttaagc aggtgatgct aaaaggggca gtgatccaac tgacgccgca
     9661 gacggaagcg gtgcgcagtg aagacgctcc ggttgcaccg cgcgacaata ccttgccgga
     9721 tctgtcagac gatcgcggat ggtcgtttga tatatccagt cttaaggtgg cggacagcgt
     9781 gctggtgttc cagcatgaag atgacgagca ggtgacaatc cgcaatattc gcctgcaaat
     9841 ggaacaagat ccccaacatc gtggttcatt tgagttctcc gggcgggtta atcgcgatca
     9901 gcgcgatctc acgatatccc ttaacggtac ggtagatgct tctgattatc cgcatgattt
     9961 aacggcggct attgaacaaa ttaactggca gttgcagggt gccgatttac caaaacaagg
    10021 tattcagggg caggggagtt tccaggccca gtggcaggag tcacataaac gcctttcatt
    10081 taaccaaatt agtttgaccg ccaatgacag tacgctgagc gggcaagcac aggttacgct
    10141 gacagagaaa ccggaatggc agctgaggct gcaattcccg caactgaatc ttgacaacct
    10201 catcccgctt aatgaaaccg cgaatggtga aaacggtgcc gcgcagcagg ggcagagcca
    10261 atcaacgttg ccgcgcccgg tcatttcttc gcgtattgat gaaccggcct atcagggact
    10321 gcaaggcttt acggctgata ttttgttgca ggccagtaac gtgcgctggc gcggaatgaa
    10381 ttttacagat gttgccacgc aaatgaccaa caagtcgggt ttgctggaaa ttactcaact
    10441 gcagggcaaa cttaacggtg gacaggtttc actgccgggc acgctggacg cgacatcaat
    10501 aaatccgcgg ataaacttcc agccacggct ggaaaacgtt gagattggta ccattctgaa
    10561 ggcgtttaac tatccgattt cgttgaccgg aaaaatgtca ctggctggtg atttctccgg
    10621 tgctgacata gatgccgacg cattccgcca caactggcaa ggacaggcac atgtcgaaat
    10681 gaccgacacg cgcatggaag ggatgaactt ccagctgatg attcagcaag cggtagagcg
    10741 taatggcggt gatgtgaagg ccgctgaaaa ctttgataac gtgacgcggc ttgaccgctt
    10801 taccaccgat ttgacgttga aggatggcgt cgtgacgtta aacgacatgc aaggtcaatc
    10861 gcctgtgctg gcgctgacag gggaaggcat gttgaatctg gcagatcaaa cctgcgacac
    10921 ccagtttgat attcgggtcg tgggtggctg gaacggggaa agcaaactga ttgatttcct
    10981 gaaagaaacg ccagtaccgc tgcgggttta tggcaactgg cagcaactca attacagcct
    11041 gcaagtggat cagttactgc gcaaacatct acaggacgaa gcgaaacgtc gcctgaatga
    11101 ctgggccgag cggaataaag attcccgtaa tggcaaagat gtgaagaagt tgcttgagaa
    11161 gatgtaggtt accaggtgac cagtcctgta ggcctgataa gacgcgtaaa gcgtcgcatc
    11221 aggcgttcgt ggcggcatca ttatattctg aatatgtaag atcgaaggct gttccgttca
    11281 cttgatgttc ctcgttacta tggaacgctc aagccggtga acgtgccagg gtggctcaat
    11341 cgccaccacc ctggcaaccc gggcccacac ccccgctgtg atgtccatgt accacagaca
    11401 acgcttcatt gctgaagtta taaggcgtta cgcagcatcg ggtaaaatga ccgataccat
    11461 cacacctcgt actccatctc gccatcctta cgcagcggta taatctgcac cttctgcacg
    11521 cgatggcttt ctacctgcaa cgttttaagc aaataatccc ccacctgaac ttcttcgcca
    11581 ggttttggaa tacgctgcaa atactccatc aacagcccgg caatggtgtg atattcacgt
    11641 ttttcatcca gcggcagcgg cacatattgc accagatcct ccagcggcat atgaccattc
    11701 gccgtccagg aaccgtccgc attcttctga atatcatggc gggcgtcgat ctcttccact
    11761 tcgttcggta agttaccggc aatggtttca gtgacgtcac ttaatgtcac aatcccttcc
    11821 accgagccaa actcatccac cacaaaagca aagtgcgtgc gggcattacg gaactgctcc
    11881 agggcaggta acaacggcaa ggtttccggg aacaccagcg gctggcgaat caacacccgc
    11941 aggttgagcg gttcgccgcg cagagactgt tgtagcaggt cgataacgtg aacaacaccg
    12001 agcaaatctt ctgcgtcatc accgtcggta accaccagcc gcgtatgctg atttcgctcc
    12061 agcaactggc ggatctcgtc ttccggcgcg ttgagatcga tatgctcaat atcgtggcgc
    12121 gacgtcataa tgctgctgac ggtacgctgg ttaagattta gtacccgctc aatcatccgc
    12181 cgttcctgcg gattaaagat ctgctggtta ccatgatcca ccagcatgga cgcggtttcg
    12241 gcgtccagtt ccgcatcttc tttttgcccg ctaagcaggc gcattaccgc ttcggtagtc
    12301 cgctggcgca gcgtctggtt agcggaaaga aaacgtcgcc ggttaaagat agccaactga
    12361 ttgagcgcct cgatcatcac cgagaaacca atggcagcgt acaggtagcc tttcggaatg
    12421 acgaagccga aaccttctgc caccaggcta aagccaatca tcaacaggaa gcttaagcag
    12481 agaataacga tcgtcgggtg actgttaaca aattgcgtta acggcttgct ggccatcaac
    12541 atcaggctga tagcgataac cacggcggcc atcatgacca gtaaatggtc gaccatcccg
    12601 acggcggtaa tcactgagtc gagtgagaaa atggcgtcca gtaccacaat ttgcgtcacc
    12661 acgccccaga acttcgcacc tttgcgttgt gtgggattat tgctgtcttt cccttccagc
    12721 cgttcgttca gctccatcgt ggctttgaac aacaggaaga aaccaccaaa cagcatgatt
    12781 aagtcgcggg cgctaaaggt aaacgagcgg aagctgaaca gcggttgagt cagggtgacc
    12841 agccaggaga ttgacgccag cagtaacagg cgcattaaca ttgccagcag cagcccggta
    12901 acccgtgcgc ggtcacgctg cttcggcggt agtttttcgg cgaggatggc aataaagacc
    12961 aggttatcaa tgccgaggac cagttcgatc acaatcagcg tgattaaccc ggcccagata
    13021 gacggatcgg caatccattc catagtaagt acaaaaccta ttcgttgtat gacaaatgtc
    13081 acaattcgat catggataaa
//
LOCUS       DAFAVF010000117        12974 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFAVF010000117 DAFAVF010000000
VERSION     DAFAVF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.117"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..523
                     /locus_tag="LGK42_003271"
     CDS             <1..523
                     /locus_tag="LGK42_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK2659494.1"
                     /translation="NLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQN
                     INLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRL
                     YRVNFDEASVQGMGISILRGEENIPIDSDTLVTRQKFFEEDCTSHTGMSQTEDNINAV
                     AMKITADIMQHAD"
     gene            complement(677..2326)
                     /locus_tag="LGK42_003272"
     CDS             complement(677..2326)
                     /locus_tag="LGK42_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBK2659495.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2477..3826)
                     /gene="ghxP"
                     /locus_tag="LGK42_003273"
                     /gene_synonym="yjcD"
     CDS             complement(2477..3826)
                     /gene="ghxP"
                     /locus_tag="LGK42_003273"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBK2659496.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4372..4836)
                     /gene="soxR"
                     /locus_tag="LGK42_003274"
     CDS             complement(4372..4836)
                     /gene="soxR"
                     /locus_tag="LGK42_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBK2659497.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4922..5245
                     /gene="soxS"
                     /locus_tag="LGK42_003275"
     CDS             4922..5245
                     /gene="soxS"
                     /locus_tag="LGK42_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBK2659498.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5248..6834)
                     /gene="pdeC"
                     /locus_tag="LGK42_003276"
     CDS             complement(5248..6834)
                     /gene="pdeC"
                     /locus_tag="LGK42_003276"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBK2659499.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSSKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7264..7545
                     /locus_tag="LGK42_003277"
     CDS             7264..7545
                     /locus_tag="LGK42_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBK2659500.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7644..8180)
                     /gene="ssb1"
                     /locus_tag="LGK42_003278"
     CDS             complement(7644..8180)
                     /gene="ssb1"
                     /locus_tag="LGK42_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBK2659501.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8434..11256
                     /gene="uvrA"
                     /locus_tag="LGK42_003279"
     CDS             8434..11256
                     /gene="uvrA"
                     /locus_tag="LGK42_003279"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK2659502.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11291..11647)
                     /locus_tag="LGK42_003280"
     CDS             complement(11291..11647)
                     /locus_tag="LGK42_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK2659503.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11651..12067)
                     /locus_tag="LGK42_003281"
     CDS             complement(11651..12067)
                     /locus_tag="LGK42_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBK2659504.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12178..12891)
                     /gene="aphA"
                     /locus_tag="LGK42_003282"
     CDS             complement(12178..12891)
                     /gene="aphA"
                     /locus_tag="LGK42_003282"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBK2659505.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDIGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3358 a         3407 c         3169 g         3040 t
ORIGIN      
        1 aaatctttat aaagtaatag cgccatatgt taatttattt aaagccgacc ttagtttctc
       61 taaactcgat ttaattaact ttgaacatgc tgatctgtct cgcgtcaatc tgaacaaagc
      121 aatcctccag aatataaact taattgatag caaactcttt tgtacgtggc taacaaatac
      181 attcctcgaa atggttatat gtaccgactc taatatggct aatgttaatt ttaataatgc
      241 caatttaagc aactgccatt tcaactgttc tattttaaca aaagcctgta tgtttaatac
      301 ccgtctctat cgggttaatt ttgatgaggc tagcgtccag ggaatgggca tttccatact
      361 ccgtggggag gaaaatatcc ccattgatag tgataccctg gtaacacgac agaaattctt
      421 tgaagaagat tgtacctctc atactggcat gtcacaaact gaggataata ttaatgcagt
      481 cgctatgaag attactgcag atattatgca acacgcagat tgaggttaaa tcgaagaaca
      541 gccaatgaat tgactttaca agagagataa cttatttgtt ttagaatcat gaatgttagc
      601 ccgagctaac ctgttaaagg tgcaaaagat tgcacctttt tcagattacc tcgggcctgg
      661 ttacgtcgct ttcggcttac tgattttcct caattagcaa cgcttcaagc aaatcgagat
      721 cgtgcagtaa tttttgcagc gtttcgttgc tgatctcccg cgtggcgcgc aggtggtaca
      781 gttcagcacg ttcagaacgc aatgccgcca gacggaagcg acgctcaagg ttctcttcct
      841 gcacggaact ttcaacgtcg ttacgtccat cggcgcgacg acgcaggtta ccaatgacgc
      901 gagaactgac ctccgtaagc agctggttat cgatattctc ttcggtatcc gccgccagac
      961 gctcctccat tttctggatc gccacaatcg ccacttctgc cgttgccgct cgcgcaatac
     1021 gttcctcttt caattgttgc gaatgatccg cgacttcaat gtgttgtagc agaataggca
     1081 acatcaccac gccgacaaac agcgagaaga gaatgacgcc agccgccagg aacaccagct
     1141 catagcgcgc cgggaagacg ttaccatccg gcaagagcag cgggatagag agcacaccgg
     1201 ccagagtgat cgccccacgc accccagcga aagacgcgat caggatttct cgtgtggtcc
     1261 acgaaccaaa ctccatcggc ttcttcttca ggaagcggtt gctgaacttt ttcatcgtcc
     1321 acagccagcc gaaacggacc agcatcagcg ccgcatatat cagaataata tcggtaaaca
     1381 gcatccagat ttcgacgtta gggtcgattt ctgccgccat cagcgacgtc tccagaatac
     1441 ccggtagctg cagacctaac agcaggaaca ccatgccgtt aaagacaaat tccagcatcg
     1501 cccaggtgct gtttgcacgc aggcgcattg ccagcggcgc acggcgcatc acaccggagc
     1561 gggtgatggt catcccggcg gcaaccgcag cgaggatgcc ggaaacgcca atatgttcgg
     1621 caatcagata agaagcgaat ggcagcagga acagcaggac gatctgcgtc gcgggttcat
     1681 caccgcccca gcggctgagg aatcgcagcg agcgaccgta cagccagcta accacaaaac
     1741 cggcgagaat accgccaatg gctactttca taaattcgac cgtcgcaccg ccgacggtga
     1801 agatcatcgt ccccatcgcc actgctacgg caaacttcaa cgacaccagg ccggaagcgt
     1861 cattcatcaa cgcttcgccc tgcaaaatgc ccattatttt tttcgggata cgcccttcgc
     1921 caacaatccc ggagagcgcc acagcatcgg tcggagaaag taccgccgcc agcgcaaagg
     1981 cggggatcag cggaatgccc ggcaccaccc agtaaataag gaagccaatg ccgaccacgg
     2041 tgaccaccac cagcgccagc gcgaggccaa aaatctctcg accatgttcg agaaattcac
     2101 gggtcggcgt tttccagcca tcagcgaaca gcaacggcgg gataaacaag actaaaaaga
     2161 gttcaggatc aaactccaca tgcaaaccaa acgtcggcca cgccagtagc gcaccgatgg
     2221 cgatttgcat aagcggaagc gggatctgaa agggcatgac acgagtgacc accccggaca
     2281 gcgagaccac gagggtcatt atcagtatgg tgaagaagat ttccatgcgt tccctgtttg
     2341 cgatgttttt tatgatctga gaaagacgtt atgcctgaat tctcattttt tcagaatatc
     2401 gcaaaagctc acacaatgtg cggagaaaat aaaaacgggc agccattgcc acccgttttc
     2461 agaatccgat gaaagattag atagcccagc cacccgcata gaaggtcacc agcgccacgg
     2521 cgataacgac cgtaccgatg ttcaacttgc gccattcgcc ggaaaccaga cgaccaatca
     2581 ccagagtcgc gaagccgatc atgatgcctg ttacgatgtt acaggtcagc acgatgaata
     2641 ccgccgtaac cagacccgcc atcgcatcaa caaaatcagc aaagtcgatt ttcgccacgt
     2701 tgctcagcat cagcaggcca acgtacatca gcgccggagc cgttgcatac cccggaacga
     2761 ggtaagagag cggagagagg aacagaatca gcaggaacag cacgccaacg gtgatagccg
     2821 tcaaaccggt tttaccgccc gccgccgtac ccgccgcaga ctcgatgtat accgctgccg
     2881 gagctgcacc caccaggcca gagaaaacgc tgctcatgga gtcagtggtc agtgctttcc
     2941 caccgtcgat gatctgccca tctttatcca gcaggttcgc ctggccggcg acggcacgga
     3001 tagttccggt ggcatcaaat actgccgtca tcaccagcgc cagaacgctt ggcaggacta
     3061 caggattcag cgcgcccata atgtccaggc tgccaatcag tgaattgccg ttttcatcgc
     3121 tcaatgaagg catggcgaaa acgccggaga aatggacgtt aggatcgaag atcaaaccga
     3181 caattgagat accgataatg gtcagcagaa tgccaccagg gactttcagt ttttccaggc
     3241 cgatgatcac cgccagacct accagtgaca taatcaccgg gaaggtcgcg aaatcaccca
     3301 gcgcaacggg cagaccatca agcgggtttt taatcaccag accgacacca ttagcggcaa
     3361 tgagcagcag gaacagaccg ataccgatcc ccgtgccgtg cgccacaccg tgaggcaagt
     3421 tgcgcaaaat ccagctacgg atacccgtgg cagaaattac cgtaaacagc acacccatca
     3481 ggaacacggc acccagcgcg acaggtacgc taatatgttg ccccagcacc aggctgaatg
     3541 cggtaaatgc cgtcagggag atggcgcaac caatcgccaa cggcagatta gcccacagac
     3601 ccatcacgat agaaccgagt ccggcaacca gacaggttgc aacgaaaact gccgcaggcg
     3661 ggaagcctgc tttacccaac atacctggaa cgacgatgac cgagtagacc atcgccagaa
     3721 acgttgttaa cccggcaacc acttcctgac ggacagtgct tccacgttgt gaaattttaa
     3781 accaggcgtc gagtgaaccg ccggtacgcg ctgatggcgt agacataaga aaacatcccc
     3841 tgagagttta attttcgtca agatgcgtgg cggacaatcc cctgccagcg aaacgttatc
     3901 ttcctgctcc aggtttgcga caatttgctg cgtcgcaaaa aagcaaacgt ttagctccgc
     3961 gcatacaaaa cgggtggtaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc
     4021 aaccgaagtt tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg
     4081 atgcgcaaac gttatcgtcg gtgcgcaaga ttgcagcgtt atcgagccgt ataccgttca
     4141 ggattgcgat aaaaaatcgt ctgagatgac aaaaatattg cggttaaaaa gtatactttt
     4201 acactggcga agaaacgtcg gtgacataca attaaagcat caacaccaac cggaacctcc
     4261 accacgtgct cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt accagcggga
     4321 tagagagaaa gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg
     4381 ttcatcttcc agcaagcgtg cgccggtacc ttcttctcct aagcggtcgc ccgggttacg
     4441 caacgggcaa tcactgcgcg aaaggcagcc acaaccaata catccgtcca gttcgtcacg
     4501 cagcgctact aaggtatgaa tgcgccgatc caactcttct cgccattggg acgaaagctg
     4561 tttccactct ttcgcactta acgtatgccc ttcgggcaac acgccaaacg cttcaccaat
     4621 ggtcgccagc ggaatgccaa tacgctgagc aattttgata attgcaacat atcgcaacac
     4681 atcacgttta tatcgccgct gattgccgct gttacggata ctggtaatca accctttact
     4741 ttcatagaaa tgcagcgccg ataccgccac accgctgcgt ttcgccactt cgccgggggt
     4801 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
     4861 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
     4921 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
     4981 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
     5041 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
     5101 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
     5161 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
     5221 cagcgattat cgccaccgcc tgtaatttta ttgcccgcgc gttaactccc gcgcgggtaa
     5281 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaga accatccctg
     5341 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
     5401 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
     5461 actggttttg tgggtggtca gcgtttcaac aaacgatttg tcgattttca aaatatcgac
     5521 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
     5581 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
     5641 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
     5701 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
     5761 aacatagcga tctgcatgtg ttgccaggta atcgcccaga tcgcggaaga cattatcaat
     5821 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     5881 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     5941 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     6001 ggcgcgttgc aatttacgtt tggaagataa atagttttgt cgaatacgta gccagagtaa
     6061 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     6121 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     6181 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     6241 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     6301 atcatacaca ccccattgca atgtcggatc atcagacatc acttcactcc agaagagagg
     6361 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     6421 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     6481 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     6541 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     6601 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     6661 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     6721 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     6781 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     6841 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     6901 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     6961 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     7021 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     7081 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagttc acaaaattaa
     7141 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     7201 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     7261 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcaacttct tagtgccgta
     7321 atgatgtttc tggccagcac actcaacatc cgttttcgtc ggtctgatta tgtcgggctt
     7381 gcggtgatca gcagcggtct gggcgttgtt tccgcctgtt ggttcgcaat ggggttgctt
     7441 ggcatcacaa tggcggacat caccgctatc tggcacaaca tcgagtcggt gatgatagaa
     7501 gagatgaatc agacaccgcc acaatggcca atgattttga cctgatacgt agaagcctcc
     7561 ataacggagg cttctttttt atggctggca gatgctttaa tcatccacct taaaacaata
     7621 taacctattg ttttaatgac aaatcagaac ggaatgtcat catcaaagtc catcggcggc
     7681 tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc gccgctgaac
     7741 tgattgccac cctgcggctg ctgtggctga ccccaaccgc cctgcggctg accaccaccg
     7801 atattgccac ctgccggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg
     7861 ccaacgttca ccacgacttc tgtggtgtag cgatcctgac cggattgatc ggtccattta
     7921 cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctcgcc
     7981 acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgttc tttcatctcg
     8041 ccggtcgctt tatcacgcca ggattcggaa gtagccagcg taatgttggc aactgcgcca
     8101 ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg
     8161 tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa atcgttctga ataagtgtaa
     8221 acgcgcgatt gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa
     8281 acaaacctgg ccagacattg ttacacaaca ctccgggtaa tgcattccaa tactgtatat
     8341 tcattcaggt caatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac
     8401 cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc
     8461 cgcacccata atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg
     8521 accgggcttt cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg
     8581 cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggaa
     8641 aagccggacg tcgatcatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg
     8701 acgtctcata acccgcgttc tacggtgggg acaatcaccg aaatccacga ctatttgcgt
     8761 ttgttgttcg cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg
     8821 caaaccgtca gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg
     8881 ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg
     8941 gcaagccagg gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg
     9001 ccgaaactgg aactgcaaaa gaaacacact attgaagtgg tggttgatcg cttcaaggtg
     9061 cgtgacgatc ttacccaacg tcttgcggag tcgtttgaaa ccgcgctgga gctttccggt
     9121 ggtacagcgg tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttctccgcc
     9181 aacttcgcct gcccaatttg cggctacagt atgcgtgaac tggagccgcg actgttttcg
     9241 tttaacaacc cggcaggtgc ctgcccgacc tgtgacggcc ttggcgtaca gcaatatttc
     9301 gatcctgacc gcgtgatcca aaaccccgag ctgtcactgg ctggcggtgc gatccgtggc
     9361 tgggatcgcc gcaacttcta ttatttccag atgctgaaat cgctggcaga tcactataag
     9421 ttcgacgtcg aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac
     9481 ggttctggca aagaaaacat tgaattcaaa tacatgaacg atcgtggcga tacctccatc
     9541 cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacagaa
     9601 tccagtgcgg tacgtgaaga attagccaag tttatcagca atcgcccatg cgccagctgc
     9661 gaagggacgc gtctgcgtcg ggaagcgcgc cacgtttatg tcgagaatac gccgctgcct
     9721 gctatctccg acatgagcat cggtcatgcg atggaattct tcaacaatct caaactagct
     9781 ggtcagcggg cgaagattgc agaaaaaatc cttaaagaga tcggcgatcg tctgaaattc
     9841 ctcgttaacg tcggcctgaa ttacctgaca ctttcccgct cggcagaaac actttccggc
     9901 ggtgaagccc agcgtatccg tctggcgagc cagattggtg cgggcctggt tggcgttatg
     9961 tatgtactgg acgagccgtc tatcggcctg caccagcgcg ataacgagcg cctgttgggt
    10021 acgcttatcc atctgcgcga tctcggtaat accgtgattg tggtggagca tgacgaagac
    10081 gcaattcgcg ccgctgacca tgtgatcgat atcggtcctg gtgcgggtgt tcacggcggt
    10141 gaagtggtcg cagaaggtcc gctggaagcg attatggcag tgcctgaatc gttgaccggg
    10201 cagtacatga gcggtaaacg caagattgaa gtgccgaaga aacgcgttcc ggcgaatcca
    10261 gaaaaagtgc tgaagctgac aggtgcacgc ggcaacaacc tgaaggacgt gacgctcacg
    10321 ctgccagtcg gtctgtttac ctgcatcaca ggggtttcag gttccggtaa atcgacgctg
    10381 attaacgaca cactgttccc gattgcccaa cgccagttga atggtgcgac catcgccgaa
    10441 ccggcaccgt atcgcgatat tcaggggctg gagcatttcg ataaagtgat cgatatcgac
    10501 caaagcccaa ttggtcgtac tccacgttct aacccggcga cctataccgg cgtgtttaca
    10561 cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga
    10621 cgtttcagct ttaacgtccg tggcgggcgc tgcgaagcct gtcagggcga cggtgtgatc
    10681 aaagtggaga tgcacttcct gccggatatc tacgtgccgt gcgatcagtg caaaggtaaa
    10741 cgctataacc gtgaaacgct ggaaattaag tacaaaggca aaaccatcca cgaagtgctg
    10801 gatatgacca tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag
    10861 ctgcaaacgt tgatggacgt tggtctgacg tacattcgcc tggggcagtc cgcaaccaca
    10921 ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc
    10981 gggcagacgc tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag
    11041 caactcctcg acgtgctgca taaactgcgc gatcagggca ataccattgt ggtgattgag
    11101 cacaatctcg acgtgatcaa aaccgctgac tggattgtcg acctgggacc agaaggcggc
    11161 agtggcggcg gcgaaatcct cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct
    11221 tcgcatacgg cacgcttcct caagccgatg ctgtaatcgt taaggccgct ttctgagcgg
    11281 ccttttcctt tcagagttgt accagcaatt tacgcttttc ttccggtagt aaattcactg
    11341 cctgctgata agacgcatcc accagataat agatttgcga atccggcagc gaaccgtcga
    11401 gatacacggt gctccagtgc gctttattca gatggcggct aggacgcaca tcgctgtgct
    11461 gctgacgtag cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt
    11521 ctacttcttt caccatcgca aacagtacat cttccacttt gatctgcgtc gctttccagt
    11581 cgttatgcac gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccgaaa
    11641 tggtcatttt ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc
    11701 gatgttcccc cagccagatg ccttgccagg tgccggtctg aatacgccct ttatgtaacg
    11761 gcaatacaag cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc
    11821 cctcatagtc atgctcataa tttccgttgt cgggaacggt gcggaggaaa aaacgctcca
    11881 tgtcgtggcg tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt
    11941 gcagcaacag atgcagtaag ccgatgttaa cgcgcggcat atcagccagc tgattcagaa
    12001 tttcatccgt taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat
    12061 accacatgtg ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag
    12121 gataaaggtg tgattaaaaa aacagcattc gggagagcat cacgctctcc cgctctgtca
    12181 gtattctgaa ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta
    12241 ggtagagttg gaggcgcgca gaatgcggat accacgagcg ccgatatcgc gtgcggcggt
    12301 aatatcgtta tcggaatcgc cataaaaaat tcggatattt ttatcctgca gccattgcga
    12361 ttttgtattt tgccctggtt tatcgcccgc aaagattact ggattcatgt tggtggcagg
    12421 aatatgaaaa ttatccgcca gcgtttttga aaccgtttct gttttcgtcg ggctacgacc
    12481 agtcacaaag aagatcgcgt caccgcggcg tacatgcata tcaatcagct ggcgagcgac
    12541 ctcttttgga atgctgaatt catcccagcc attgttcatt ttttcccaga acacaggatt
    12601 tttcagataa tcttcgcttt ctggtgagaa ggtttttttg ccgcgccaga agcccgggct
    12661 ggaaaaaagt accgtgtcat cgatatcaaa ccccaccgcc attggcggac gccctgcgag
    12721 gctattttca atttgtgcga ccgaaaccca atgaattggt gcctgttcag caagcctggc
    12781 aacgttagtc ccagggttaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt
    12841 tagagcgaac aataagcaaa cggcactgat tgcctgtgtg atcttgcgca tatttttccc
    12901 taaatagtca gtatgttgaa actttttatt gtgagatttg ttgcaaaaac catctgacca
    12961 taacgccagc tgcc
//
LOCUS       DAFAVF010000118        12894 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFAVF010000118 DAFAVF010000000
VERSION     DAFAVF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.118"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(80..856)
                     /gene="yaaA"
                     /locus_tag="LGK42_003283"
     CDS             complement(80..856)
                     /gene="yaaA"
                     /locus_tag="LGK42_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBK2659506.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(926..2356)
                     /locus_tag="LGK42_003284"
     CDS             complement(926..2356)
                     /locus_tag="LGK42_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBK2659507.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2635..3588
                     /gene="tal"
                     /locus_tag="LGK42_003285"
     CDS             2635..3588
                     /gene="tal"
                     /locus_tag="LGK42_003285"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBK2659508.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANSDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3703..4290
                     /gene="mog"
                     /locus_tag="LGK42_003286"
     CDS             3703..4290
                     /gene="mog"
                     /locus_tag="LGK42_003286"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBK2659509.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4325..4891)
                     /gene="satP"
                     /locus_tag="LGK42_003287"
     CDS             complement(4325..4891)
                     /gene="satP"
                     /locus_tag="LGK42_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBK2659510.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="LGK42_003288"
     CDS             complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="LGK42_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBK2659511.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5779..6183)
                     /locus_tag="LGK42_003289"
     CDS             complement(5779..6183)
                     /locus_tag="LGK42_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBK2659512.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6560..8476
                     /gene="dnaK"
                     /locus_tag="LGK42_003290"
     CDS             6560..8476
                     /gene="dnaK"
                     /locus_tag="LGK42_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK2659513.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8565..9695
                     /gene="dnaJ"
                     /locus_tag="LGK42_003291"
     CDS             8565..9695
                     /gene="dnaJ"
                     /locus_tag="LGK42_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK2659514.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="LGK42_003292"
     CDS             complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="LGK42_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBK2659515.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            10537..11703
                     /gene="nhaA"
                     /locus_tag="LGK42_003293"
     CDS             10537..11703
                     /gene="nhaA"
                     /locus_tag="LGK42_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBK2659516.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11769..12668
                     /gene="nhaR"
                     /locus_tag="LGK42_003294"
     CDS             11769..12668
                     /gene="nhaR"
                     /locus_tag="LGK42_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBK2659517.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(12707..>12894)
                     /locus_tag="LGK42_003295"
     CDS             complement(12707..>12894)
                     /locus_tag="LGK42_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBK2659518.1"
                     /translation="GFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNN
                     VSSGQIMSRVKIVVMYN"
BASE COUNT         3314 a         3145 c         3324 g         3111 t
ORIGIN      
        1 aattctgcaa tatattgaat ctgcatgctt ttgtaggccg gataaggcgt tcacgccgca
       61 tccggcattg gctacaaact taacgctgct cgtagcgttt aaacaccagt tcaccattgc
      121 tggaggaagc ttcatcaaag aagtaacctt cgctattaaa accagtcagt tgctctggtt
      181 tggtcagccg attttcaata atgaaacgac tcatcagacc gcgtgctttc ttagcgtaga
      241 agctgatgat cttaaatttg ccgttcttct catcgaggaa caccggcttg ataatctcgg
      301 cattcaattt cttcggcttc accgatttaa aatactcatc tgacgccaga ttaatcacca
      361 cattatcgcc ttgtgctgcg agcgcctcgt tcagcttgtt ggtgatgata tctccccaga
      421 attgatacag atctttccct cgggcattct caagacggat ccccatttcc agacgataag
      481 gctgcattaa atcgagcggg cgcagtacgc catacaagcc ggaaagcatt cgcaaatgct
      541 gttgggcaaa atcgaaatcg tcttcgctga aggtttcggc ctgcaagccg gtgtagacat
      601 cacctttaaa cgccagaatc gcctggcggg cattctccgg cgtgaaatct ggctgccagt
      661 catgaaagcg agcggcgttg atacccgcca gtttgtcgct gatgcgcatc agcgtgctaa
      721 tctgcggagg cgtcagtttc cgcgcttcat ggatcaactg ctgggaattg tctaacagct
      781 ccggcagcgt atagcgcatg gtggtcaacg ggctttggta atcaagcgtt ttcgcaggtg
      841 aaataagaat cagcatatcc agtccttgca ggaaatttat gccgacttta gcaaaaaaag
      901 agaatgagtt gatcgatagt tgtgattact cctgcgaaac atcatcccac gcgtccggag
      961 aaagctggcg accgatatcc ggataacgca acggatcaaa caccgggcgc acgccgagtt
     1021 tacgctggcg tagataatca ctggcaatgg tatgaaccac aggcgagagc agtaaaatgg
     1081 cggtcaaatt ggtaatagcc atgcaggcca ttatgatatc tgccagttgc cacatcagcg
     1141 gaagacttag caaggtgccg ccgatgaccg ttgcgaaggt gcagatccgc aaacaccaga
     1201 tcgctttagg gttgttcagg cgtaaaaaga agagattgtt ttcggcataa atgtagttgg
     1261 caacgatgga gctgaaggca aacagaataa ccacgagggt aacaaactca gcaccccagg
     1321 aacccattag cacccgcatc gccttctgga taagctgaat accttccagc ggcatgtagg
     1381 ttgtgccgtt acccgccagt aatatcagca tggcgcttgc cgtacagatg accagggtgt
     1441 cgataaaaat gccaatcatc tggacaatcc cttgagctgc cggatgcgga ggccaggacg
     1501 cagctgccgc tgctgcgttt ggtgtagaac ccattcccgc ctcattggaa aacatactgc
     1561 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
     1621 cctgccagcc aaaagcactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
     1681 tcattacgca aatcaccagg ctggtcagta cccagattat cgccatcaac gggacaaaac
     1741 cctgcatgag ccgggcgacg ccatgaagac cgcgagtgat tgccagcaga gtaaagacag
     1801 cgagaataat gcctgtcacc agcgggggaa aatcaaaaga aaaactcagg gctcgggcaa
     1861 cggcgttcgc ctgaactccg ctgaaaatta tgccataggc gatgagcaaa aagacggcga
     1921 acagaacgcc catccaacgc atccccagcc cgcgcgccat ataccatgcc ggtccgccac
     1981 gaaactgtcc attgacgtca cgttctttat aaagttgtgc cagagaacat tcggcaaacg
     2041 aggtcgccat gccgataaac gcggcaaccc acatccaaaa gacggctcca ggtccaccgg
     2101 cggtaattgc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
     2161 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
     2221 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
     2281 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
     2341 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
     2401 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
     2461 tacatgaggt tttgtataaa aatggcgggc gatatcaacg cagtgtcaga aatccgaaac
     2521 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     2581 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     2641 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     2701 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     2761 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcttgggc gaaacagcag
     2821 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     2881 ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc gcgtctttcc
     2941 tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgct
     3001 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     3061 gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt ctccttcgct
     3121 caggctcgtg cttgtgcgga agcaggcgtg ttcctgatct cgccatttgt tggccgtatt
     3181 cttgactggt acaaagcgaa tagtgataag aaagagtacg ctccggcaga agatccgggc
     3241 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aacagtggtt
     3301 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg cgaccgtctg
     3361 accatcgcac cggcactgct gaaagagctg gcggagagcg aaggggctat cgaacgtaaa
     3421 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     3481 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     3541 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     3601 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     3661 tatcattctg tttaacgaga ctgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     3721 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcatccct
     3781 gcgctggaag aatggctgac atcggcgcta accacgccgt ttgaactgga aacccgctta
     3841 atccccgatg agcaggcgat catcgaacaa acgttgtgtg agctagtgga tgaaatgagt
     3901 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     3961 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagatc
     4021 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg tgggcgtgat tcgcaaacag
     4081 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     4141 aaggacgctg cgggtaacgt tgtggtacac ggtatttttg ccagcgtacc gtactgcatt
     4201 cagttgctgg aagggccata cgttgaaacg gcaccggaag tggttgcagc attcagaccg
     4261 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
     4321 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
     4381 cacccatcgc cagatagatt gcgctggcac cgcagatcag cccaatccag ccggcaaagt
     4441 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacggtca
     4501 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
     4561 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     4621 tcagacccag tttcggcatc agcagaatcg caaccagcgt cagccagaaa gaaccgtaag
     4681 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccagcaaaaa
     4741 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     4801 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     4861 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     4921 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     4981 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     5041 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     5101 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
     5161 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     5221 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     5281 tctttcatca gcagcggcag cagctcttcc agctcattca ccctggcatc gatcgcgtgc
     5341 agaaactcct gcttatgttc ctcgtccata ttcttccagg tgttacgcag aaattgttcc
     5401 agtaactgtt gctcaatctc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     5461 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     5521 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     5581 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtac gctggagagg
     5641 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     5701 ctgcaatgct ggagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     5761 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     5821 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcact
     5881 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcagact
     5941 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccgcttaact gtaaatcgcc
     6001 atggtcggca gagagttgta tgcgtttcac aatgcgacaa acaggaagtt tcagcgccag
     6061 atcgttggtt tcgttacgcg gcattgcaat gacgccgagg agtttatggt cgtttgcctg
     6121 cgccgtgcag cacagcatca ggctaatcgc caggctggcg gaaatcgtaa aaacggattt
     6181 cataaggatt ctcttagtgg gaagaggtag agggatgaat acccactagt ttactgctga
     6241 taaagagaag attcaggtac gtaatctttt ctttttatta caattttttg atgaatgcct
     6301 tggctgcgat tcattcttta tatgaataaa attgctgtca attttacgtc ttgtcctgcc
     6361 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     6421 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatggaattg
     6481 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     6541 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     6601 ttgtgtagcg attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg
     6661 caccacgcct tctatcattg cctataccca ggatggtgaa actctggttg gtcagccggc
     6721 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     6781 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     6841 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     6901 gatctctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
     6961 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     7021 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     7081 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
     7141 cctgggtggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     7201 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
     7261 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     7321 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     7381 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
     7441 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     7501 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     7561 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     7621 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     7681 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     7741 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     7801 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     7861 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     7921 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     7981 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     8041 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     8101 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     8161 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     8221 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     8281 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     8341 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     8401 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     8461 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     8521 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     8581 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     8641 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     8701 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     8761 acgatcagta tggtcatgct gcgtttgagc aaggtggtat gggcggcggc ggttttggcg
     8821 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     8881 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     8941 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     9001 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     9061 gttctggtca ggtgcagatg cgtcagggtt tctttgccgt gcagcagacc tgtccacact
     9121 gtcagggtcg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     9181 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     9241 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     9301 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg
     9361 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     9421 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac tggtaagctg ttccgtatgc
     9481 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttactg tgccgcgttg
     9541 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     9601 aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg
     9661 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     9721 aggcctgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
     9781 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     9841 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     9901 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     9961 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc
    10021 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
    10081 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttctgtttta
    10141 tcgattctaa tcctgaagac gcctcgcatt tttatggcgt aattttttaa tgatttaatt
    10201 atttaacttt aatttatctc ttcatcgcaa ttattgacga caagctggat tatttttgaa
    10261 atattggcct aacaaacatc gccgactgac aacaaattaa ttattacttt tcctaattaa
    10321 tctctcagaa atcctcacct taagctatga ttatctgggc ttagggtcac tcgtgagcgc
    10381 ttacagccgt caaaaactca tctcaccgct gatggcgcaa attcttcaat ggatcgtaaa
    10441 aaacgagtta ttcctacact ataatctgat tttaacgatg atttgtgcgg ggtaaaatag
    10501 taaaaacgat ctattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt
    10561 agcagtgatg cctcgggagg cattattctt atcattgccg ctatcctggc gatgattatg
    10621 gccaatagcg gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc
    10681 cgggttggtt cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg
    10741 gcggtatttt tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta
    10801 gccagcttac gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg
    10861 gcattactct atctggcctt taactatgcc gatccgatta cccgcgaagg gtgggcgatc
    10921 ccggcggcta ctgacattgc ctttgcactt ggtgtgctgg cgctgttggg aagtcgtgtt
    10981 ccgttagcgc tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc
    11041 attatcatcg cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct
    11101 gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat
    11161 attctggttg gcgtagtgtt gtggactgcg gtgttgaaat cgggggttca cgcaaccctg
    11221 gcgggggtaa ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg
    11281 aagcgactgg agcatgtgtt acacccgtgg gtggcgtatc tgattttgcc gctgtttgca
    11341 tttgctaatg ctggcgtttc actgcaaggt gtcacgctgg atggcttgac ctccattctg
    11401 ccattgggga tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc
    11461 tggttggcgc tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt
    11521 atggcggtgg ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg
    11581 gcctttggta gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt
    11641 tctatctctt cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtt
    11701 tgacaggacg gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag
    11761 aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag
    11821 aaggttccgt ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac
    11881 agattcgagc gctggaagag cgcctgcaag gcaaattatt taaacgcaag ggacgtggtc
    11941 tcgaacccag cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa
    12001 gccaggaaat gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg
    12061 gtgtggctga tgcactttcc aaacgcctgg tcagtagcgt acttaacgcc gcagtggtag
    12121 aaggcgagcc cattcatctt cgctgcttcg aatccaccca cgaaatgctg ctggagcaat
    12181 taagtcagca taaactggat atgatcattt ctgactgtcc gatagactct acgcagcagg
    12241 aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac
    12301 caccagaaaa accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac
    12361 gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa
    12421 tcctcggcga gtttgatgat gccgcgttga tgaaagcttt tggtgcgatg cacaatgcaa
    12481 tcttcgttgc cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa
    12541 ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc
    12601 agcacccggc ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg
    12661 cgcgttaatc ggcacctccc cccaaagtta aggtggggga gatagattag ttgtacataa
    12721 ccacgatttt gactcggctc attatttgcc cgcttgagac attatttcca tatgtacgcg
    12781 ggcgaataaa tagaggaatc tgattacttc cttcatgggg atgctgaaaa gagtagtaat
    12841 tgcttttacc atactggatt tcattcgtac catcattgga gagttgaaag ccta
//
LOCUS       DAFAVF010000119        12873 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFAVF010000119 DAFAVF010000000
VERSION     DAFAVF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.119"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..537
                     /locus_tag="LGK42_003296"
     CDS             <1..537
                     /locus_tag="LGK42_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2659519.1"
                     /translation="SNNWQKQKRKIQRLHSRIAYIRRDYLHKVTTTVSKNHAMIVIED
                     LKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVP
                     PAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMV
                     QSGRPLKQEPTEMIQATA"
     gene            complement(577..1791)
                     /gene="ydcO"
                     /locus_tag="LGK42_003297"
     CDS             complement(577..1791)
                     /gene="ydcO"
                     /locus_tag="LGK42_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBK2659520.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1844..2380
                     /gene="sutR"
                     /locus_tag="LGK42_003298"
     CDS             1844..2380
                     /gene="sutR"
                     /locus_tag="LGK42_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBK2659521.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2453..4414
                     /gene="rlhA"
                     /locus_tag="LGK42_003299"
     CDS             2453..4414
                     /gene="rlhA"
                     /locus_tag="LGK42_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBK2659522.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4506..4736)
                     /locus_tag="LGK42_003300"
     CDS             complement(4506..4736)
                     /locus_tag="LGK42_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBK2659523.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4958..5134
                     /gene="hicA"
                     /locus_tag="LGK42_003301"
     CDS             4958..5134
                     /gene="hicA"
                     /locus_tag="LGK42_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HBK2659524.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5180..5596
                     /gene="hicB"
                     /locus_tag="LGK42_003302"
     CDS             5180..5596
                     /gene="hicB"
                     /locus_tag="LGK42_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBK2659525.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5675..7081
                     /locus_tag="LGK42_003303"
     CDS             5675..7081
                     /locus_tag="LGK42_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK2659526.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7326..8471
                     /locus_tag="LGK42_003304"
     CDS             7326..8471
                     /locus_tag="LGK42_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2659527.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8489..9502
                     /locus_tag="LGK42_003305"
     CDS             8489..9502
                     /locus_tag="LGK42_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2659528.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9503..10444
                     /locus_tag="LGK42_003306"
     CDS             9503..10444
                     /locus_tag="LGK42_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2659529.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTTFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10434..11228
                     /locus_tag="LGK42_003307"
     CDS             10434..11228
                     /locus_tag="LGK42_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2659530.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAARRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11250..12674
                     /gene="patD"
                     /locus_tag="LGK42_003308"
     CDS             11250..12674
                     /gene="patD"
                     /locus_tag="LGK42_003308"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBK2659531.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12771..12866)
                     /gene="yncL"
                     /locus_tag="LGK42_003309"
     CDS             complement(12771..12866)
                     /gene="yncL"
                     /locus_tag="LGK42_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBK2659532.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         3143 a         3176 c         3447 g         3107 t
ORIGIN      
        1 agcaacaact ggcagaagca gaaacgcaaa atacagcgac tgcattcccg tatcgcatat
       61 atccgcaggg actaccttca caaagtcaca acgaccgtca gcaaaaacca cgcaatgata
      121 gtcattgagg atttgaaggt cagcaacatg tcaaagtcag cagcgggtac ggtcagccag
      181 ccggggcgca atgtccgggc aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg
      241 tatgaaatgc gccgccagct tgcgtacaag cagctctggc gtggcggtca ggtgcttgct
      301 gttccgccag cgtatacaag ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaat
      361 cgtctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
      421 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agggatggtg
      481 cagtcaggcc gcccgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      541 tagcagggat ccacgtcctt cagggcgtgg aggatgtcaa tatcggtttc tgtcagcgat
      601 taaattcaac accacgtaac aaacgcctcc ggcaattaat ccccagaacg cagaaccaat
      661 cccgaccagc gtcaatccac ttgccgttac cagaaatgcc accaccgccg cgtcgcgctc
      721 acgctcatta tgcagcgcct gatacaaact gccgccaatg gtactcaaca gcgccagacc
      781 tgccagcatc tggatccaac ttacgggcag ggcagccatc atcccggtaa tggcactacc
      841 aaacagacct gcgagcaaat agaaaatgcc tgcaccggca gctgccagcc aacgttgatc
      901 tttatccgga tgcgcttccg ggctttggca aatagccgcg gtgattgccg caataccgac
      961 ggaataaacg ccgaaagggg aaaaaaccag tgccagcaat ccagtaaata caattaatgg
     1021 cgaaacagga gttgaatatc cggctgcttt cattgctgcg atacccggtg cgttttgcga
     1081 tgccatcgtc accagaaaaa gggggagtgc aacgctcagg ctgtgagcaa acgaaaaatc
     1141 aggggtaata taagtgggga gaacgggttt aaagacaaca tcagttgtga caacgtcacc
     1201 ttgcgcgata acgatcacga tcccaataat catcgcggca attaccgcat agcgtggcgc
     1261 aacggccttg gttgccagcc ataccagcaa catacttcca cacaacgtaa attgaccgtc
     1321 cagactggca aacgcctgta aaccaaagcg taataaaatc ccggcaagca ttgccgccgc
     1381 aagcgagtgc ggaataatgc gcatcagacg agcaaagagt cccgttatgc cgcagaggac
     1441 tattaacgcg ttggtgacaa taaaaacgcc gatggcttcg ttaagtgtta gtccctgcaa
     1501 tccggtgacc aacaaagccg cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata
     1561 ccataatgtc agagtcagcg tactgacgcc cattgccagc cccagcgccg tcatccagcc
     1621 agagatttgt gcagtggtgg ctccggcgac aatcgctgct tgccagatta ttgccgctga
     1681 actggcgtag ccaattaata ccgccagaaa ccccgccagt agcgtgggtg gaggaacaga
     1741 aaacagacgc atagtcacct cgtgcgttat agcgtacagg caccgtagca tttgtccgtt
     1801 ataacgcaca agtgataaac ttccgttttg ccggaggagt cgcatggaaa atctcgctcg
     1861 ctttttatcc accacactta aacaactacg ccagcagcgc ggctggagtc tttcgcgact
     1921 ggcagaagcg acgggcgttt ctaaagcaat gttagggcag attgagcgta atgagtccag
     1981 tccgacggtc gcgacattat ggaaaattgc caccgggttg aacgtaccat tttcaacatt
     2041 tatttctccg ccgcagcccg ctacgccttc agtttatgat ccacaacagc acgcgatggt
     2101 gatcacttcg ctgtttcctt atgatccaca actctgcttt gaacacttct cgattcagat
     2161 ggcatctggt gcaataagcg aatcgacgcc ccatgagaag ggggtaatcg aacatgtcgt
     2221 cgtcattgat ggacaacttg atctgtgcgt tgatggggaa tggcagaccc ttaattgtgg
     2281 ggaaggcgtt cgatttgctg cagacgtcac gcatatctat cgtaatggtg gggagcaaac
     2341 cgtacatttt cattccctca tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa
     2401 cgtcgcgctt gtggctaaaa tagccgccat ttttcagcta ctggataaga atgtgaccgt
     2461 atcttctcat cgacttgaac tgttaagccc ggcacgcgat gccgccattg cccgcgaagc
     2521 tattttgcac ggtgccgatg ctgtttatat cggcggccct ggttttggtg cccgtcataa
     2581 tgccagtaat agcctgaaag atattgccga gctggtgccg tttgcccatc gttatggtgc
     2641 aaaaattttc gtcacactta acaccatttt gcatgatgat gagctggaac ccgcgcaacg
     2701 gctgattacc gacctctacc agaccggtgt cgatgcgctg attgttcagg atatggggat
     2761 tctggaactt gatattccgc cgattgaact gcacgccagt acgcagtgcg acattcgtac
     2821 agtagaaaaa gcgaagttcc tctctgatgt tggcttcacg cagattgtgc tggcgcgaga
     2881 gctgaatctt gagcagatcc gcgcgattca ccaggccacg gacgcgacca ttgaattctt
     2941 tattcatggg gcactgtgcg tggcctattc gggtcagtgc tacatttctc atgcgcaaac
     3001 agggcgtagc gccaaccgtg gtgattgctc gcaggcgtgc cgtttgccat acacattgaa
     3061 agacgatcag gggcgggtgg tttcctatga aaaacatctg ctgtcgatga aagataacga
     3121 tcagactgcc aacctcggcg cgctgattga tgctggcgta cgctccttca agattgaagg
     3181 gcgttacaaa gatatgagct acgtgaagaa tatcaccgcc cattatcgcc agatgcttga
     3241 tgccattatt gaagaacgtg gcgatctggc gcgcgcttca tcaggtcgta ctgaacattt
     3301 ctttgttcca tcgacggaaa agactttcca ccgtggtagc acagattatt ttgtgaatgc
     3361 ccgtaaaggc gatattggcg cgttcgattc gccgaaattt atcggcctgc cggtaggcga
     3421 agtattgaaa gtggcgaaag atcatctcga tgttgccgtc accgagccac tggcaaatgg
     3481 tgatggcctg aacgtgatga ttaaacgtga agtcgtcggt tttcgtgcca atacggtcga
     3541 gaaaaccgga gaaaatcagt accgcgtctg gcccaatgaa atgccagcag atttgcacaa
     3601 aattcgtcca catcacccac taaaccgtaa tcttgatcat aactggcagc aggcactgac
     3661 aaaaacctcc agcgaacgtc gggtggcggt agacattgaa ctgggcggct ggcaggaaca
     3721 actgattctg accctcacca gtgaagaggg tgtcagcatc acgcatacgc tggacgggca
     3781 gttcgacgaa gccaataacg ccgaaaaagc aatgaacaat ctgaaggatg gtctggcaaa
     3841 actggggcaa accctctatt acgcccgcga tgtgcaaatt aatttgccgg gggcgctgtt
     3901 tgtaccaaac agtctgttaa accagttccg ccgtgaagct gctgacatgc tggatgctgc
     3961 gcgtcttgcc agttaccagc gcggcagccg taaaccggtt gctgatcctg cgccggttta
     4021 tccgcaaacg catctgagtt tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta
     4081 tcatcgctat ggtgtgcagc tgattgacgc ggcgtatgaa gcacatgaag agaagggcga
     4141 agtcccggtg atgatcacca agcattgtct gcgctttgcc tttaatctgt gcccgaaaca
     4201 ggcgaaaggc aatatcaaaa gctggaaggc gacgccaatg caactggtta acggcgatga
     4261 agtattaacg ctaaagtttg attgccgccc atgcgagatg cacgtcattg gcaaaatcaa
     4321 aaatcacata ctgaaaatgc cgttaccggg aagcgtagtg gcatccgtaa gtccggatga
     4381 gctgctgaaa acattgccta agcgaaaagg gtaaaacgcc agttttctgg ttactcacaa
     4441 ctcattgaat ctgcatgata ttgcctgccg ggtaaggcgt ctcgccgcat ccggcaacaa
     4501 atgactcaac gttgtcccgc ttctggtttg cgcgattttt gccagtaaaa atgctcgcgc
     4561 aaaccttccg ccgactcttc cgccacagca cgcaattcat cgctgtccgc ttcatgacgc
     4621 agctgatgat ccacattctt tacccacaca aattcatgtc ctttgtgccc tgccatgagt
     4681 tgtcctgaga acagcgcaca cgttaataag acaaccgata acgccttcgt aaacatcctg
     4741 ccaccttttt cttacctttt gccgctatga tgccgatcgt ttcttgaggt tattattcag
     4801 ttttgcaaat tagcgcaaag aaattctgga atcttccttc ctgattttgc atggcattct
     4861 gccgttgcgg cgatttagtg ctattttcga gcaaattaca cacggaggta aacactaatg
     4921 tttactttgt tgatatactc agcggcaggg aggcgatgtg aaacaaagcg agttcagacg
     4981 ttggctcgaa tctcagggcg tcgatgtagc gaatggcagc aaccatttga aactcaggtt
     5041 tcatgggagg cgcagtgtca tgccgcgtca cccctgcgat gagattaaag aaccattgcg
     5101 taaagcaatc ctgaaacaac tcggtttgag ttaatcgcca attaaaaagg ttaatgacat
     5161 gcgagggaca gtcgaaatta tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg
     5221 ttatatggtt tcttttgtgg atatccctga agcgttgacg cagggcgaaa ctgtcgctga
     5281 agcgatggaa gcggcaaaag atgctttact gaccgcattt gatttttatt ttgaagataa
     5341 cgagcttatc ccgttacctt cgccattaaa tagtcatgat cactttattg aagtaccttt
     5401 gagcgtcgcc tctaaagtat tgctgttaaa tgctttttta cagtcagaaa tcactcagca
     5461 agagttagcc aggcgaattg gcaaacctaa acaggagatt actcgcctat ttaacttgca
     5521 ccatgcgaca aaaatcgacg ccgtccagct tgcggcaaag gcgcttggca aagagttatc
     5581 gctggtgatg gtttaattac agttaacgaa aagttgtcat ttttaacaac tgatatagac
     5641 tgccgaatca tctgcacata attacgattc gataatgaaa aaataccagc agcttgcaga
     5701 acaattacgc gagcagattg cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt
     5761 gcgtgaccag gtggcgcttt caggcatgag ctttatgact gtcagccatg cctatcagtt
     5821 gctcgaaagt cagggatata ttatcgcacg accgcagtcg ggttattacg ttgcgccaca
     5881 ggcaataaaa atgccgaaag cgccagtcat tccagtcact cgagatgaag cagtcgatat
     5941 caacacttat atttttgata tgttgcaggc cagtcgcgat ccgtcggtcg ttccgtttgc
     6001 ctcggccttt cccgacccgc gacttttccc cctccaacaa ctaaaccgct cgctggcgca
     6061 ggtaagcaaa accgccacgg cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga
     6121 actgcgtcag gctattgctc gtcgctatgc cttacagggc atcaccattt ctcctgatga
     6181 aattgtcatt actgccgggg cgttagaggc attaaacctc agtttgcaag cggtaactga
     6241 accgggcgat tgggtgatag tagagaatcc ttgtttctat ggcgcgttgc aggcgctgga
     6301 acggctacgg ctgaaagcgt tatcggtggc gacggatgtt aaagaaggga tagatcttca
     6361 ggcgttggaa ctggcgttgc aggagtatcc ggtgaaagcg tgctggctga tgactaatag
     6421 ccagaatcca ctcggattta ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact
     6481 caatcagtac aacgtaacgc tgattgaaga tgacgtttac agcgaacttt attttggacg
     6541 ggaaaaaccg ctgcctgcga aagcgtggga tcgccacgat ggcgttttgc attgctcttc
     6601 gttttcgaaa tgtctggtgc ctggttttcg tattggttgg gtcgccgccg gaaaacatgc
     6661 acgtaaaatt caacgcttgc agttgatgag tacgctttcc accagctcac cgatgcagct
     6721 tgcgctggtg gattaccttt ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg
     6781 ccagcttgcg gaacgtaaac aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga
     6841 agttaaaatt catcatagtg acagcggtta ttttctctgg ctggagctcc ccgagccgtt
     6901 agatgccggc gaattaagcc tggcggcact gacgcatcat atcagtattg cgccgggtaa
     6961 aatgttttct accggtgaaa actggtcacg tttttttcgt tttaataccg cgtggcagtg
     7021 gggagaacgt gaagaacagg cggtaaaaca attaggcaaa cttattcaag aacggctgta
     7081 atagcgttta atttaattcc tcttagattg ggtaatatga atttcgaata gcagtcatat
     7141 ttcctaaccc cttgtctata ctccagaaga taaccttaca gacggcataa tgcgcggtag
     7201 ctcacaacct gattaaattt tctcaggggc gaaggtgtgc ctgcaagccg ccgtctatgg
     7261 ttaaacaagg agatattttt acggcacggc ggctgaacaa ttaattacga caggagtaag
     7321 accttatgag caagacattt gcccgcagca gcctgtgtgc gctcaccatg acaataatga
     7381 ccgctcacgc cgccgaacca cctaccaatt tagataaacc ggaagggcga ctggatatta
     7441 tcgcctggcc gggatacatc gaacgcggac aaactgataa acaatacgac tgggtaacgc
     7501 aatttgaaaa agagacaggc tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa
     7561 tggtcagtct gatgaccaaa gggggttacg atctggttac ggcatccggc gatgcctcgc
     7621 tgcgtttgat tatgggcaaa cgcgtgcagc cgattaatac cgccttgatt cccaactgga
     7681 aaacgctcga tccgcgcgtg gttaaaggcg actggtttaa tgttggcggc aaagtttacg
     7741 gcacacctta ccaatggggg ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc
     7801 cgccggatag ctggcaagtg gtttttgttg agcaaaatct gccggacggc aagagcaata
     7861 aaggccgcgt tcaggcttat gatggcccta tctacattgc ggacgctgcg ttgttcgtta
     7921 aagccactca gccgcagttg ggcatcagcg atccgtatca actcaccgaa gaacagtacc
     7981 aggcggtgct gaaagtgctg cgcgctcaac acagtttgat ccatcgctac tggcatgaca
     8041 ctaccgtgca aatgagcgat ttcaaaaacg agggtgtggt tgcttccagt gcctggccct
     8101 atcaggccaa cgccctgaaa gccgaaggcc agcctgttgc taccgttttc ccgaaggagg
     8161 gggttaccgg ttgggctgat accaccatgc tgcatagcga agcgaaacat ccggtttgcg
     8221 cctacaaatg gatgaactgg tcattaacgc caaaagtgca gggcgatgtg gcggcctggt
     8281 ttggctcgtt accggtagtg ccggaagggt gtaaagccag tccgttatta ggcgaaaaag
     8341 gttgtgaaac caacggtttt aactatttcg acaaaatcgc cttctggaaa acgcctatag
     8401 cagaaggggg caagtttgtt ccctacagtc gctggacgca ggattacatt gccattatgg
     8461 gcggtcgcta acttcgctgg ggtgctttat gacgtacgca gtggagtttg acaacgtctc
     8521 gcggttgtac ggtgacgtgc gcgcagtaga tggcgtcagt attgcgataa aagatggtga
     8581 gttcttctct atgctggggc cgtccggctc cggcaaaacc acctgcctgc gcctgattgc
     8641 tggcttcgaa cagctttccg gcggggctat ctctatcttt ggtaaacccg ccagcaatct
     8701 gccaccgtgg gagcgggacg tgaatactgt ctttcaggac tacgcgctat ttccgcatat
     8761 gtcgattctt gacaatgtcg cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg
     8821 gcacgcaatg gcgcaagagg cgctggagaa agtggcgttg gggtttgtac atcaacgtaa
     8881 accgtcacaa ctttctggtg gtcagcgcca gcgggttgct atcgccagag cattggtgaa
     8941 tgaaccgcgc gtattgctgt tggatgaacc gctcggcgca ctggatctca aattgcgtga
     9001 gcagatgcag ctggaactga aaaaactgca acagtctctc ggtatcactt ttatcttcgt
     9061 tacccacgat cagggcgaag cgttatcgat gtccgatcgt gtggcggttt tcaataatgg
     9121 acgcattgag caggtcgatt ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt
     9181 tgccggattc gtcgggacat ccaacgtttt tgatggactg atggcagaga aactttgtgg
     9241 catgacggga agcttcgccc tgcgaccgga acatatccgc ctcaacaccc ctggtgaact
     9301 gcaggccaat ggcacgatcc aggcggtgca atatcagggc gcggcaactc gttttgaact
     9361 gaaattgaac ggcggtgaaa aactgcttgt gagtcaggcc aatatgacag gcgaagaact
     9421 gcctgccacg ctcacgcccg gacaacaggt gatggtttcc tggtcgcgtg atgtcatggt
     9481 gccgctggtt gaggagaggt gaatggcgat gaatgtattg caatcacctt cacgtccagg
     9541 tctgggtaag gtgtccggtt tcttctggcg taatccgggg ctggggctgt ttttactgct
     9601 gcttggcccg ctaatgtggt ttggcattgt ttatttcggc tcgctgctaa cgctgttatg
     9661 gcagggcttt tatacctttg acgatttcac catgtcggta acgccggaac tgacgctggc
     9721 gaatattcgt gcgctgttta atccggcgaa ttacgacatc attctccgca cgctgactat
     9781 ggctgtggca gtcactatcg ccagcgccat tctggctttt ccaatggcgt ggtatatggc
     9841 gcgctatacc agcgggaaaa tgaaagcgtt tttttatatt gcggtaatgt tgccgatgtg
     9901 ggcgagctac attgttaaag cctatgcctg gacgttattg ctggcaaaag atggcgtggc
     9961 tcagtggttt ttacaacatc ttgggctgga accactgctg actacgttcc ttacattacc
    10021 tgcggtgggc ggcaatacgc tgtcaacttc cgggctgggg cgctttctgg tgtttctcta
    10081 tatctggctg ccgttcatga tcctgcccgt gcaggcggcg cttgagcgtt tgccgccgtc
    10141 attgttgcag gcgtcggctg atctcggcgc acgtccacga caaacctttc gctatgtggt
    10201 gctgccgctg gcaatcccgg gtattgccgc tggctctatc tttaccttct cactcacact
    10261 gggcgatttt atcgtcccgc agctggttgg tcctccagga tattttatcg gcaatatggt
    10321 ttattcccag cagggggcga ttggcaatat gccgatggcg gcggcattca ccctggtgcc
    10381 gattgttctc atcgcactgt acctggcgtt cgtgaaacgt ctgggagcgt tcgatgcact
    10441 ctgaacgcgc accgtttttc ctcaaactgg cggcctgggg cggcgttgtt ttcctacatt
    10501 ttcccatcct gataatcgcc gcctatgcgt ttaacactga agatgcggcg tttagttttc
    10561 caccgcaggg cctgacgctg cgctggttta gcgtggcagc acggcgtagt gatattcttg
    10621 atgccgtgac actgtcactt aaagtggcgg cgctggcgac attaattgcg ctggtgttag
    10681 ggacgctggc agctgccgcg ctgtggcgac gagacttttt cggcaaaaac gccatttcgc
    10741 tgttactgct gctgcccatt gcgctgccgg gcattgtcac tggtctggcg ctattaaccg
    10801 cttttaaaac catcaatctg gagccgggat ttttcaccat cgtggtcggt catgcgactt
    10861 tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt tcgccgcacc tcgtggagtc
    10921 tggttgaggc gtcaatggat cttggggcca atggctggca aaccttccgc tacgtagtgt
    10981 tgccgaatct cagttcggcg ctactggcag gaggaatgct ggcgtttgcc ttgtcgttcg
    11041 atgaaatcat cgttacgact tttacggcag gtcatgaacg aacgttaccg ttgtggttgc
    11101 tcaatcagct tgggcgaccg cgtgatgtac cggtaactaa cgtggtggca ctgctggtta
    11161 tgttggtaac aaccttgccg atcctggggg cctggtggct aacccgcgaa ggcgacaatg
    11221 gtcaataacc actgatacag gaatatgcta tgcaacataa gttactgatt aacggagaac
    11281 tggttagcgg cgaaggggaa aaacagcctg tctataatcc ggcaacgggg gacgttttac
    11341 tggagattgc cgaggcatcc gcagagcagg tcgatgctgc tgtgcgcgcg gcagatgcag
    11401 catttgccga atgggggcaa accacgccga aagtgcgtgc ggaatgtctg ctgaaactgg
    11461 ctgatgttat cgaagaaaat ggtcaggttt ttgccgaact ggagtcccgt aattgtggca
    11521 aaccgctgca tagtgcgttc aatgatgaaa tcccggcgat tgtcgatgtt tttcgctttt
    11581 tcgcgggtgc ggcgcgctgt ctgaatggtc tggcggcagg tgaatatctt gaaggtcata
    11641 cttcgatgat ccgtcgcgat ccgttggggg tcgtggcttc tatcgcaccg tggaattatc
    11701 cgctgatgat ggcagcgtgg aaactggctc cggcgctggc ggcagggaac tgcgtagtgc
    11761 ttaaaccatc agaaattacc ccgctgaccg cgttgaagtt ggcagagctg gcgaaagata
    11821 tcttcccggc aggcgtgatt aacgtactgt ttggcagagg caaaacggtg ggtgatccgc
    11881 tgaccggtca tcccaaagtg cggatggtgt cgctgacggg ctctatcgcc accggcgagc
    11941 acatcatcag ccataccgcg tcgtccatta agcgtactca tatggaactt ggtggcaaag
    12001 cgccagtgat tgtttttgat gatgcggata ttgaagcagt ggtcgaaggt gtacgtacat
    12061 ttggctatta caatgctgga caggattgta ctgcggcttg tcggatctac gcgcaaaaag
    12121 gcatttacga tacgctggtg gaaaaactgg gtgctgcggt ggcaacgtta aaatctggtg
    12181 cgccggatga cgagtctacg gagcttggac ctttaagctc gctggcgcat ctcgaacgcg
    12241 tcagcaaggc agtagaagag gcgaaagcga cagggcacat caaagtgatc actggcggtg
    12301 aaaagcgcaa gggtaatggc tattactatg cgccgacgct gctggctggc gcattacagg
    12361 acgatgccat cgtgcaaaaa gaggtatttg gtccagtagt gagtgttacg cccttcgaca
    12421 acgaagaaca ggtggtgaac tgggcgaatg acagccagta cggacttgca tcttcggtat
    12481 ggacgaaaga tgtgggcagg gcgcatcgcg tcagcgcacg gctgcaatat ggttgtacct
    12541 gggtcaatac ccatttcatg ctggtaagtg aaatgccgca cggtggacag aaactttctg
    12601 gttacggcaa ggatatgtca ctttatgggc tggaggatta caccgtcgtc cgccacgtca
    12661 tggttaaaca ttaaggataa tattgcagat cgtaagagta tgagatgatc ttgcgtactg
    12721 tcagagagtt gatttctctg acagtacgtg ctgcgtgcaa aatcactaca tcaaataaac
    12781 cagccaaatc tcatagagat aagagtaaac aaaccaacag cagcaaagac atttatcagt
    12841 actacagttc tactggaaac attcattttt gcc
//
LOCUS       DAFAVF010000120        12845 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFAVF010000120 DAFAVF010000000
VERSION     DAFAVF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.120"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            128..1759
                     /gene="oppA"
                     /locus_tag="LGK42_003310"
     CDS             128..1759
                     /gene="oppA"
                     /locus_tag="LGK42_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBK2659533.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1845..2765
                     /gene="oppB"
                     /locus_tag="LGK42_003311"
     CDS             1845..2765
                     /gene="oppB"
                     /locus_tag="LGK42_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBK2659534.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2780..3688
                     /gene="oppC"
                     /locus_tag="LGK42_003312"
     CDS             2780..3688
                     /gene="oppC"
                     /locus_tag="LGK42_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBK2659535.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3700..4713
                     /gene="oppD"
                     /locus_tag="LGK42_003313"
     CDS             3700..4713
                     /gene="oppD"
                     /locus_tag="LGK42_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBK2659536.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4710..5714
                     /gene="oppF"
                     /locus_tag="LGK42_003314"
     CDS             4710..5714
                     /gene="oppF"
                     /locus_tag="LGK42_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBK2659537.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5767..6096)
                     /locus_tag="LGK42_003315"
     CDS             complement(5767..6096)
                     /locus_tag="LGK42_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBK2659538.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6131..7591)
                     /gene="cls"
                     /locus_tag="LGK42_003316"
     CDS             complement(6131..7591)
                     /gene="cls"
                     /locus_tag="LGK42_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK2659539.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7734..7907
                     /locus_tag="LGK42_003317"
     CDS             7734..7907
                     /locus_tag="LGK42_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBK2659540.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7962..9215)
                     /gene="kch"
                     /locus_tag="LGK42_003318"
     CDS             complement(7962..9215)
                     /gene="kch"
                     /locus_tag="LGK42_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBK2659541.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9514..9810)
                     /locus_tag="LGK42_003319"
     CDS             complement(9514..9810)
                     /locus_tag="LGK42_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBK2659542.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10034..10753
                     /gene="tonB"
                     /locus_tag="LGK42_003320"
     CDS             10034..10753
                     /gene="tonB"
                     /locus_tag="LGK42_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBK2659543.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(10793..11191)
                     /gene="yciA"
                     /locus_tag="LGK42_003321"
     CDS             complement(10793..11191)
                     /gene="yciA"
                     /locus_tag="LGK42_003321"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBK2659544.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11296..11835)
                     /locus_tag="LGK42_003322"
     CDS             complement(11296..11835)
                     /locus_tag="LGK42_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBK2659545.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(11865..12608)
                     /locus_tag="LGK42_003323"
     CDS             complement(11865..12608)
                     /locus_tag="LGK42_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBK2659546.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILHKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12636..12725)
                     /locus_tag="LGK42_003324"
                     /pseudo
     CDS             complement(12636..12725)
                     /locus_tag="LGK42_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3427 a         3065 c         3161 g         3192 t
ORIGIN      
        1 ggtgatagta atacgtaacg ataaagtaac ctgacagcag aaagtctccg agcctgtgca
       61 gggtcccaat ccgggattac acatgctggt taataccagt aattataatg agggagtcca
      121 aaaaacaatg accaacatca ccaagagaag tttagtagca gctggcgttc tggctgcgct
      181 aatggcaggg aatgtcgcgc tggcagctga tgtacccgca ggcgtcacac tggcggaaaa
      241 acaaacactg gtacgtaaca atggttcaga agttcagtca ttagatccgc acaaaattga
      301 aggtgttccg gagtctaata tcagccgaga cctgtttgaa ggcttactgg tcagcgatct
      361 tgacggtcat ccagcacctg gcgtcgctga atcctgggat aataaagacg cgaaagtctg
      421 gaccttccat ttgcgtaaag atgcgaaatg gtctgatggc acgccagtca cagcacaaga
      481 ctttgtgtat agctggcaac gttctgttga tccgaacact gcttctccgt atgccagtta
      541 tctgcaatat gggcatatcg ccggtattga tgaaattctt gaagggaaaa aaccgattac
      601 cgatctcggc gtgaaagcta ttgatgatca cacattagaa gtcaccttaa gtgaacccgt
      661 tccgtacttc tataaattac ttgttcaccc atcaacttca ccggtgccaa aagccgctat
      721 cgagaaattc ggcgaaaaat ggacccagcc tggtaatatc gtcaccaacg gtgcctatac
      781 cttaaaagat tgggtcgtaa acgaacgaat cgttcttgaa cgcagcccga cctactggaa
      841 caacgcgaaa accgttatta accaggtaac ctatttgcct attgcttctg aagttaccga
      901 tgtcaaccgc taccgtagtg gtgaaatcga catgacttat aacaacatgc cgatcgaatt
      961 gttccagaag ctgaaaaaag agatcccgga cgaagttcac gttgatccat acctgtgcac
     1021 ttactattac gagattaaca accagaaacc gccattcaac gatgtgcgtg tgcgtaccgc
     1081 actgaaacta ggtatggacc gcgatatcat tgttaataaa gtgaaagcgc agggcgacat
     1141 gcccgcctat ggttacactc caccgtatac tgatggcgca aaattgactc agccggaatg
     1201 gtttggctgg agccaggaaa aacgtaacga agaagcgaaa aaactgctgg ctgaagcggg
     1261 ttataccgca gacaaaccgt tgaccatcaa cctgttgtat aacacctccg atctgcataa
     1321 aaagctggcg attgctgcct cttcattgtg gaagaaaaac attggtgtaa acgtcaaact
     1381 ggttaaccag gagtggaaaa cgttcctcga cacccgtcac cagggtactt ttgatgtggc
     1441 ccgtgcaggc tggtgtgctg actacaacga accaacttcc ttcctgaaca ccatgctttc
     1501 gaacagctcg atgaataccg cgcattataa gagcccggcc tttgacagca ttatggcgga
     1561 aacgctgaaa gtgactgacg aggcgcagcg cacagctctg tacactaaag cagaacaaca
     1621 gctggataag gattcggcca ttgttcctgt ttattactac gtgaatgcgc gtctggtgaa
     1681 accgtgggtt ggtggctata ccggcaaaga tccgttggat aatacctata cccggaatat
     1741 gtacattgtg aagcactaat ggcaatacgt ggggcaggag tgtcctgctc cacggtgtct
     1801 gatttttatc gcattacaga aggcacaggc cagaaggtag ggcaatgtta aaatttattc
     1861 tacgtcgctg tctggaagcg attccgacgc tatttattct tattactatt tcgttcttta
     1921 tgatgcgcct cgcgccggga agccctttta ccggcgaacg tactttaccg cctgaagtga
     1981 tggccaatat cgaagcgaaa tatcatctta atgatccgat catgacacag tatttcagct
     2041 acctgaaaca actggcgcac ggtgatttcg gtccatcgtt taaatataaa gattattcgg
     2101 tcaatgacct ggtggcatcc agttttcccg tttctgccaa actgggagcc gcagcatttt
     2161 tccttgcggt aatactgggt gttagtgctg gcgttattgc cgcattaaaa caaaacacca
     2221 aatgggacta taccgtgatg gggctggcaa tgaccggggt tgttatcccc agttttgtgg
     2281 ttgcgccatt attagtcatg atatttgcga tcattttgca ttggctgccg ggcggtggct
     2341 ggaatggtgg ggcgcttaaa ttcatgatat tgccgatggt ggcgttgtca ctcgcctata
     2401 tcgccagtat tgcgcgtatt acccgtggct ctatgattga agtattacac tccaatttta
     2461 ttcgtactgc ccgggcgaaa gggttaccta tgcggcggat cattttacgc cacgcattaa
     2521 aacctgctct gttacccgtg ctctcctata tgggccccgc atttgtcggc attattaccg
     2581 gttctatggt tatcgaaacc atttatggtt tgccggggat tgggcaactg ttcgttaatg
     2641 gcgcattgaa ccgtgactat tccttagtgt taagcctgac catcctggtt ggtgctttaa
     2701 ccattttgtt taatgccatt gtcgatgtgc tatatgcggt tatcgacccg aaaatccgtt
     2761 actgatactg gagctcgcga tgatgttaag taagaaaaac agcgagacgc tggaaaattt
     2821 cagtgaaaag ctggaggtcg aagggcgcag cttgtggcag gacgcacgtc gacgttttat
     2881 gcataaccgt gcggcggttg ccagtctgat agtgctggtg ctgatcgcgt tatttgtaat
     2941 cctggcaccg atgctttcgc agtttgccta tgacgatact gactgggcga tgatgtccag
     3001 cgccccggat atggagtccg gtcactactt tggtactgac tcatccggtc gcgacctgct
     3061 tgtgcgcgtt gcgattggcg ggcgtatctc actcatggtc ggtgttgctg cggcactggt
     3121 ggcagtggtc gtcgggacac tttatggttc gctttccggt tatctgggcg gtaaagtgga
     3181 ttcggtaatg atgcgtctgc tggaaatcct caactccttc ccattcatgt tcttcgtcat
     3241 tttgctggtg acctttttcg gtcaaaacat cctgctgatt ttcgtggcga taggcatggt
     3301 ttcctggctg gatatggctc gtattgtgcg tgggcaaacc ctgagtctga agcgcaaaga
     3361 gtttattgag gcggcacaag ttggcggtgt atcgacatcg ggcattgtta ttcgccacat
     3421 tgtgccgaat gtactcggtg tggtggtggt ctacgcatca cttctggtgc ccagcatgat
     3481 cctctttgaa tctttcctta gcttcctggg gttgggtacg caagagccgt taagcagctg
     3541 gggtgcattg ctgagtgatg gcgcgaactc gatggaagtc tctccatggt tattgttgtt
     3601 cccagcggga ttcctcgtgg tgacgctgtt ttgtttcaac tttatcggcg atggcttgcg
     3661 tgatgccctc gacccgaaag atcgttaagg agtgcagcca tgagcgtaat tgaaactgca
     3721 actgtgccgc tcgcacaaca acaggctgac gcactgctga acgtgaaaga tttgcgtgtc
     3781 acctttagta ccccggacgg cgacgtcacg gcggtcaatg atttgaattt ttccctacgt
     3841 gccggagaaa cgctgggtat tgtaggtgag tctggttcgg gtaaatcgca aactgcattt
     3901 gcgttgatgg gcctgttggc tgccaacgga cgtattggcg gatcggcaac cttcaatggg
     3961 cgtgaaatcc tcaatttgcc agagcatgaa ctcaataaac tgcgcgctga acaaatctca
     4021 atgatttttc aggacccaat gacttcgttg aatccctata tgcgcgtcgg tgagcagtta
     4081 atggaagtgc tgatgctgca taagaacatg agcaaagctg aggcgtttga agagtcggtg
     4141 cggatgctcg atgcggtaaa aatgccggaa gcgcgtaaac gcatgaaaat gtacccgcac
     4201 gaattttctg gcggcatgcg tcagcgagtc atgattgcga tggcattgct atgtcgacct
     4261 aagctactga ttgcggatga acccactact gcgctggacg tcaccgtaca ggcgcagatc
     4321 atgacgctat taaatgaact gaagcgggaa tttaataccg ccatcattat gatcacccac
     4381 gatcttggcg tggtggcggg gatctgcgac aaagtgctgg taatgtacgc cgggcgcacg
     4441 atggaatatg gcaatgcgcg cgatgtcttt tatcaacccg ttcatcctta ttctatcggt
     4501 ttgctcaacg cggtgccgcg tcttgatgcg gaaggtgaaa caatgttgac catcccgggt
     4561 aatccgccaa acttgctgcg attaccgaaa ggttgcccgt tccagccacg ttgtccacat
     4621 gcgatggaaa tttgtagtag cgcaccgccg ctggaagagt ttacgcctgg ccgtctgcgt
     4681 gcttgcttta aaccggtgga ggaactgtta tgaatgctgt aactgaagga agaaaagtcc
     4741 tccttgaaat cgccgatctt aaagtgcact ttgaaatcaa agatggcaaa cagtggttct
     4801 ggcaaccgcc gaaaacgctc aaagccgtcg atggtgtcac tcttcgcctg tatgaagggg
     4861 aaacattagg tgtggtaggg gaatcgggat gcggtaagtc cacctttgct cgcgccatca
     4921 tcggtttggt caaggcgacc gacggtcatg ttgcctggtt aggtaaagag ttgctgggca
     4981 tgaagcccga tgaatggcgt gccgttcgca gtgatattca gatgattttc caggatccgt
     5041 tggcatcgct aaacccgcgt atgaccatcg gcgagatcat cgctgaacca ctgcgtactt
     5101 atcatccgaa aatgtcacgc caggaagttc gcgagcgcgt gaaggcgatg atgctgaaag
     5161 tcgggttatt gcctaacctg attaaccgct atccgcatga gttctccggt gggcagtgcc
     5221 agcgtatcgg gattgctcgt gctcttattc ttgaaccgaa gctgattatc tgcgatgagc
     5281 cggtttcggc gctggacgtg tcaattcagg cgcaggtggt caacctgctc cagcagctgc
     5341 aacgtgagat gggattgtca ttaattttta tcgctcatga cctggccgtg gtaaaacaca
     5401 tttccgatcg tgtgttggtg atgtatctcg gccatgcggt agaactgggg acctatgatg
     5461 aggtctacca caatccacta catccttaca ccagggcatt gatgtcggca gtccccatac
     5521 ctgatccgga tctggagaag aacaaaacca tccagttact ggaaggggaa ttaccgtcgc
     5581 cgatcaaccc gccttccggt tgtgttttcc gtacccgttg cccgattgcc ggtccggagt
     5641 gcgccaaaac acgtcctgtt ctggagggga gtttcagaca cgccgtttct tgcctgaaag
     5701 tcgatccgct ttaaacacaa taagggctga cagttgtcag ccctttttca cgctaaaagc
     5761 gattatttat tcccgccaga tgatatggca aagtttgtga tctttttcgc ggcataacag
     5821 aatgcgggcg aaaacatcgt tgatttcacc atcttcactg tccgccagac caatcaccac
     5881 ttcggcaaaa aagtctgggt tcaaatcgaa atcaacatgt tcctgccagt cttccgccgg
     5941 atcgaataac tccgcgccgc cgcgctcttc aaactgaaga ttgaacagca gaacatcagc
     6001 tggatcgaga ttgtcggcag ccagttcgag aaaaatatcg taggcctgct caagcgtttc
     6061 atcttcagtc aggcgattgt ttagatccat atccatagtc actacctgtt taacctctgt
     6121 tggcgacgtt ttacagcaac ggactgaaga agtaaaacag tcgctcggcg acacgttgcc
     6181 ataatggacg ttttagccat aaacgggcat cgagcagacg tgaacgcgaa atataatcgt
     6241 cctgaacggc ggcaaggtct gcaccaaaac ctttatcgtc gattgccagg gtaatctcga
     6301 aattcagcca cagactacgc atatcaaggt taacggtgcc aaccagactt agttcgccat
     6361 cgaccagcac gctcttggta tgcagtaacc cgccttcaaa ctgataaatt ttaaccccag
     6421 cagcaagaag ttccgtaaag aatgcgcgac tggcccagcc gaccagcatc gagtcatttt
     6481 ttcgcggaag gataatactg acatccaccc cgcgctgcgc cgccgtgcaa atcgcatgaa
     6541 gtaaatcatc gcttggcaca aagtagggcg tggtcatgat caaatattca cgcgccgaat
     6601 aagccgcagt caataatgcc tggtgaatga gatcttccgg aaagccgggg ccagaagcaa
     6661 ttgtgtgaat ggtgtgaccg ctggcctgtt caaacggcat aatattgaca tctggtggtg
     6721 gcggcagaat acgttttccg gtttcaatct cccagtcgca ggaataaata atccccatcg
     6781 cggtggcgat ggggccttcc atacgcgcca tcagatcaat ccattgccct acgcccgcat
     6841 cttgtttgaa gtagcgagga tcgaccatat tcatgctgcc ggtgtacgcg atgtaattat
     6901 cgatcatgat catcttgcga tgttggcgca ggtccatacg gcgtaaaaac acacgcatca
     6961 gattgacctt taaggcttcg accacttcaa taccggcatt acgcattagc tcgggccacg
     7021 ggctgcggaa aaaagccaca ctcccggcgg agtcgagcat caatcggcaa tgaataccac
     7081 gccgtgcagc cgccattaat gattcagcca cctggtccgc catgccgccg ggctgccaga
     7141 tataaaacac catctcaata ttatggcgcg cgagctggat gtcgcggatt aacgcctgca
     7201 tcacatcatc tgactcggtc atcagttgta gctgattccc tttgacccca gcgatcccct
     7261 gacgacgctc gcaaagcttg aataatggcg cagcgacact gctattttct tcggcgaaga
     7321 tatgcttaca ggctttaagg tcgttaagcc attttgcggt ggaaggccac atcgctctgg
     7381 cgcgctcagc gcggcgtttg cctaaatgga gctcgccaac ggcaagatag gcaataattc
     7441 cgactaacgg cagaatgtaa ataatcaaca gccaggccat cgcggaggga actgcgcgtc
     7501 gtttcattag aatgcgtaaa gttacgcctg caatgagcaa ccagtatccc agaatggcca
     7561 accaactcac caacgtataa acggttgtca tagattagaa atccttttga aagcgcattg
     7621 ttatgagttt acgcatatgg attcatctgg caaataaaaa cgcgggaaaa gcactggtca
     7681 gcgtcggggg tgggggtata atgaccattc tgttattgca tagagtagtt aacatgaagc
     7741 ggagtagaac ggaagtgggg cgctggcgca tgcagcgtca ggctagccga cgtaaatcgc
     7801 gttggcttga ggggcaatcg cgccgaaata tgcgtatcca cagcatcagg aagtgcattc
     7861 taaacaaaca gcgtaactcg ttattgtttg cgatctacaa tatctaaata tactgggcac
     7921 cattgcggtg cccaataagt ttactctttc gtctttaaac tctatttttg cactgattct
     7981 ttactgtcag ctttcgtttc ttgttcatcg ttatcgctga aaatgccatg accggagttc
     8041 aacaacattg aaacgagcat atcattatta atctcttcac catttaaaac tcgcgccaga
     8101 atttcgctgc caaacagttg cggtgagaga atgatatccg gatgtaccat cttaatctta
     8161 ttcaggtttt tgctatcact gacggcgaga actgttttga catcactgct catatctttt
     8221 gccgagagta caacaaacgc gttatctgca tcgttatcac tcagcgccag aatggcccgg
     8281 caacgatcga ttcccgcttt ctttaatact gaactgtcat tactgtcacc ggggataaca
     8341 tcagcgttat cgcctaagcg ttgctcaagt tgcttgatat catcttcagg caagttgctg
     8401 ataaccgtta cgttttgtcc gcgttgattc agttgcagga tcgtattgat ggcgagaatc
     8461 gaatgtccgc aaacaataaa atgatcttta cgatgcattg tatgattgtt tccttttaca
     8521 agtttgttga atcccccgcg gataagcgga ccaaaaattg aggtcataga tgtggcaaaa
     8581 acggtaatgc cggaaataat gaccgaaata gtgaacaatc gtgctgattc agaaacaggg
     8641 acaatatcgc catagccgac ggttgacatg gtttctatcg aaaaatagaa cgcggtcatc
     8701 aaactttcta ttcgcggatt aaaaccttcg cttaaataaa gcgcaccgta ggttgagtaa
     8761 aacagtaatg tcgtgaaact aataaatgca aaaattgtcc cggctgcggc gctactgtgg
     8821 gagaagtctt tgcgcagtat cagcaaaaac accagcgtaa aaatgcaaaa tccaatacta
     8881 aatttcagcc agggataaaa atgcagggtg taaattagcg ctatcaacaa caaaatgata
     8941 ctgattgccc atgcgagctt tgcccggaac agtaagccaa tggagttcag gacaagaaag
     9001 acgccgagca taaatagtgg ggcgtttgcc agcaaggaga gatccaactc tgaaaatgct
     9061 ttgatatgga aaatatcaag caaattgacc gacataccat agattgtttt aaaaataagc
     9121 aatccattta aaaagacggc cagcgcgaga atgtcgtgcc gtagggtcac ccataaattt
     9181 gttgctgttt gtttgaatgt agcccagtga ctcactgata cctctgtaac cacaattttg
     9241 tttaattatc ttagcgtgca cattttctga acatacatgc agcgcgtgaa tgtgttaaac
     9301 aatagtagat gactttatcg gcagtaatca aaggttttga gcgagcaact ctgcgtcagg
     9361 aaagggcatt ttcctgaata tgttcacatc atataaagaa atacagataa agttattatc
     9421 tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag aacacggttg
     9481 caaaaaccgt gcccttaaat attgaatctc tattcagaac actttcttaa atggtttcac
     9541 tgaaacgtgt tcatagacac ctgccgctac gtacgggtca gcatcggccc aggcctgagc
     9601 tgcttccagc gattcaaatt cagcaataac ggttgagcca gtaaatcccg cagcccctgg
     9661 atcgttactg tctaccgctg gcattggacc agctgtcaac aaacgacctt catcatgcag
     9721 taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga ggctatcagc
     9781 tttgtcttga gcgtaaataa catacaacac gggcacaact ccttgttcgg gaaagttgta
     9841 aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt tgttaagacc
     9901 atgcataaag taaggtcaat tacgccaaaa atgacatttt cactgatcct gatcgtcttg
     9961 ccttattgaa tatgattgct atttgcattt aaaatcgaga cctggttttt ctactgaaat
    10021 gattatgact tcaatgaccc ttgatttacc tcgccgcttc ccctggccga cgttactttc
    10081 ggtctgcatt catggtgctg ttgtggcggg tctgctctat acctcggtac atcaggttat
    10141 tgaactacct gcgcctgcgc agccaatttc tgtcacgatg gttgcgcctg ctgatctcga
    10201 accgccacaa gccgttcagc cgccaccgga gccggtggta gagccagaac cggaacctga
    10261 gccgatcccc gaaccgccaa aagaagcacc ggtggtcatt gaaaagccga agccgaaacc
    10321 taagccaaaa ccgaagccgg tgaaaaaggt acaggagcag ccaaaacgcg atatcaaacc
    10381 cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcgcgcc tgacatcaag
    10441 tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac cacgcgcatt
    10501 aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg aagggcaggt
    10561 taaagttaaa tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa tcctctcagc
    10621 caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg atgcgcagat ggcgttatga
    10681 gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta acggcaccac
    10741 cgaaattcag taagcagaaa gtcaaaagcc tccgaccgga ggcttttgac tattactcaa
    10801 caggtaaggc gcgaggtttt ccttcaggat caaccgcgac atacttaaat aatgcttctg
    10861 tcgctttata gcgttgcccg attggttcag acgctacttt tttcacccac acttcaatat
    10921 taatgctgac cgatgtcgtc cctttctgga cacagcgtgc atagcagcac accacatcgc
    10981 cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacagtcact acgcgaccgt
    11041 gggcaatttc tttcgccaga atagcgccgc caatatccat ttgtgacatt aaccaaccac
    11101 caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt aaaacaagat
    11161 cgccctgagg gacgttatgt gttgtagaca tggtaaaacc gacttaaaag gaaaatcaca
    11221 ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc cagccccgaa
    11281 ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga tatagatacc
    11341 gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa agttgaccca
    11401 aatattttgc ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa ggataaagaa
    11461 aacagcccaa gccagattta gcttcgacca taccggttgc ggcagcgtga gttctttacc
    11521 cagcatccgc tgaattagcg gctttttcat cacccattgg ctgactaaca gggctcccgc
    11581 aaacagagca taaatgactg taaccttcca tttaataaac tcatcattgt ggaagaacag
    11641 cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct tctcaacctt
    11701 acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca gcgccgcagt
    11761 agccgcgtag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg gtaaaaaatc
    11821 aagaaactgc ttcattttac gattccgtaa tcaaatgctt aaggttattg gcgaatcaac
    11881 atatacaggc ggaacagata gatgagcaat atggcagaaa tcaggttgct caaggtgttc
    11941 gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa aagcagcaac
    12001 agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat attcgcccaa
    12061 gtcagccgca tactgctacg catcgaggca aaaatgccca ttttgtcctg taccagcatc
    12121 accggtgcca gggccagtaa aatggccatg ataattcccg gaacgaccac cagcatgatg
    12181 ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataacttgtg caatatcggc
    12241 gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac cagctggata
    12301 attaatatta cgcctccggc gagaatggcg ttaccgatta atcctgaaaa agtggacgcc
    12361 gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac caggtcgaac
    12421 aacccactac tgccgctaac gggcacgccg tcattgagct gcgcaagctg tgcatcactg
    12481 ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga taccaacaga
    12541 atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga ctgcgccgtg
    12601 atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa ttgtaccctg
    12661 gttaagcctc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt gtaaagcgga
    12721 ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag tggcgatcga gttaatattt
    12781 gcgtagagaa aatatttaaa aattgattta aatcacatta accaggattc tcaatgcaac
    12841 ttcta
//
LOCUS       DAFAVF010000121        12814 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFAVF010000121 DAFAVF010000000
VERSION     DAFAVF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.121"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            184..1698
                     /gene="caiT"
                     /locus_tag="LGK42_003325"
     CDS             184..1698
                     /gene="caiT"
                     /locus_tag="LGK42_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBK2659547.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1729..2871
                     /gene="caiA"
                     /locus_tag="LGK42_003326"
     CDS             1729..2871
                     /gene="caiA"
                     /locus_tag="LGK42_003326"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBK2659548.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3000..4217
                     /gene="caiB"
                     /locus_tag="LGK42_003327"
     CDS             3000..4217
                     /gene="caiB"
                     /locus_tag="LGK42_003327"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBK2659549.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4291..5844
                     /gene="caiC"
                     /locus_tag="LGK42_003328"
     CDS             4291..5844
                     /gene="caiC"
                     /locus_tag="LGK42_003328"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBK2659550.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5953..6738
                     /gene="caiD"
                     /locus_tag="LGK42_003329"
     CDS             5953..6738
                     /gene="caiD"
                     /locus_tag="LGK42_003329"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBK2659551.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6744..7334
                     /gene="caiE"
                     /locus_tag="LGK42_003330"
     CDS             6744..7334
                     /gene="caiE"
                     /locus_tag="LGK42_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBK2659552.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7420..7815)
                     /gene="caiF"
                     /locus_tag="LGK42_003331"
     CDS             complement(7420..7815)
                     /gene="caiF"
                     /locus_tag="LGK42_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBK2659553.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8076..11297)
                     /gene="carB"
                     /locus_tag="LGK42_003332"
     CDS             complement(8076..11297)
                     /gene="carB"
                     /locus_tag="LGK42_003332"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK2659554.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11315..12463)
                     /gene="carA"
                     /locus_tag="LGK42_003333"
     CDS             complement(11315..12463)
                     /gene="carA"
                     /locus_tag="LGK42_003333"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK2659555.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3026 a         3323 c         3383 g         3082 t
ORIGIN      
        1 agcgccctct attcacccga aacaaaaatg tgataacaat cacagaatgc agcttattga
       61 atacccatta tgagttagcc attaacgcgt ccacgaggtt aataataatt atattaaatg
      121 ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt cgttttcaca gtggaattaa
      181 ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccgtta
      241 ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcagc gaatgtcgtt
      301 attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg
      361 gtggtgatgc ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta
      421 ggtaacgaac cacctgaatt tagtaccgcc agttggatct ttatgatgtt cgcctcctgt
      481 acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg
      541 ccgtttggct tagaaccgaa ctcgacaggg gcgaaagagt tggggctggc ttacagcttg
      601 ttccactggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac
      661 ttcttctttg tccgcaaaat ggaagtgatt cgccccagct cgacactggt gccgctggta
      721 ggtgaaaaac acgccaaagg gttgttcggc actatcgtcg acaacttcta tctcgtcgcc
      781 ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg
      841 caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg
      901 attatcctca acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt
      961 gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcattgt cagcggtgcc
     1021 agcttcatca tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcga
     1081 atgttgttct ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc
     1141 tactgggcat ggtgggtgat ttacgccatc cagatgagta tcttcctcgc ccgcatctcc
     1201 cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga tggggctgac agcatccacc
     1261 tggatcctgt ggaccgtgct cggtagtaac actctgctgt tgatggataa aaacatcatc
     1321 aacattccaa atctgatcga acagtacggt gtggcgcgcg ccatcatcga aacctgggcc
     1381 gctctgccac tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc
     1441 gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt ccacttgccg cgaagtacgc
     1501 gatggtgaag aaccaccgct gctggtgcgt atcggttggt caattctggt tggcattatc
     1561 ggtattgttc tgctggcgct cggcggcctg aaaccgattc aaaccgccat tatcgccgga
     1621 ggatgcccgc tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa
     1681 cagaactgga aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat
     1741 ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac
     1801 tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa
     1861 gcactggcgg atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac
     1921 gcggggtttg ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc
     1981 tatgtactgt accagttgcc gggcgggttc aacaccttcc tgcgcgaagg cacacaagag
     2041 cagatcgaca agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt
     2101 accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat
     2161 ggtaagattt atcttaatgg tagtaagtgt tttattacca gcagcgccta caccccgtac
     2221 atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt
     2281 gttgatatga gcaaaccggg catcaaagtg accaaacttg aaaagctcgg tctgcgtatg
     2341 gatagctgct gtgaaatcac ctttgacgac gtggaactgg acgagaaaga catgttcggt
     2401 cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta
     2461 gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac
     2521 cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc
     2581 cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca
     2641 gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg
     2701 gcatttgaag tggtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac
     2761 caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtctccgg gggatctgac
     2821 gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc aataccgcta agtcaatgcg
     2881 ctgcctgatg cgacgctgat gcgtcttatc aggcctacgg gagcacgaat gtaggccgga
     2941 taaagcgttt acgccgcatc cggcagtcat gcgctacata aatttttcag gagcaaataa
     3001 tggatcatct acccatgccg aaattcgggc cgttggccgg attgcgcgtt gtcttctccg
     3061 gtatcgaaat cgccggaccg tttgccgggc aaatgttcgc agaatggggc gcggaagtta
     3121 tctggatcga gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcaac
     3181 tctcccgccg caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag
     3241 cgtttctgaa attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct
     3301 ttgcccgtcg tggcattacc gatgaagtac tgtggcagca caacccgaaa ctggttatcg
     3361 ctcacctgtc cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata
     3421 acactatcgc ccaggccttt agtggttacc tgattcagaa cggtgatgtt gaccagccaa
     3481 tgcctgcctt cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc
     3541 tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt
     3601 atgaagtgat gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa
     3661 tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc cggttgcggt ctgtataaat
     3721 gcgccgacgg ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta
     3781 aagatattgg cctcgcacat ctactcggta cgccggaaat cccggaaggc actcagctta
     3841 tccaccgtat cgaatgccct tacggcccac tggtagaaga aaaactcgat gcctggctgg
     3901 cggcacatac catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca
     3961 aagtactgac cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca
     4021 ctcagtggca aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca
     4081 aaaataaccc cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca
     4141 ttttgaaaaa tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg
     4201 ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat
     4261 aacaaacgaa caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
     4321 caaatgtggg acgatcttgc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
     4381 agcggcggag tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg
     4441 gcaaacctgt tttatacgct ggggattcgc aaaggcgaca aggttgcact acatctcgac
     4501 aactgcccgg aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg
     4561 ccgattaacg cccgcctgtt acgcgaggaa agcgcgtgga tcctgcaaaa tagccaggcg
     4621 tgcctgctgg tgaccagtgc gcaattctat cctatgtatc aacagattca gcaggaagat
     4681 gccactcaat tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggcgtg
     4741 agttcgttta ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
     4801 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt
     4861 gtggtgatta cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
     4921 ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt
     4981 actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc
     5041 gcccgcgcct tctggggaca ggtgcagaag taccgcgcca ccattaccga atgtattccg
     5101 atgatgatcc gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg
     5161 cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagatgcgtt ttgtgaacgc
     5221 ttcggcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
     5281 gatcgccctg gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa
     5341 gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt
     5401 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
     5461 gcgaaagtgc tggaagccga cggctggcta cataccggcg ataccggata ctgcgacgaa
     5521 gagggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
     5581 gtctcctgcg tggagctgga aaatatcatc gccacccatc caaaaattca ggatatcgtg
     5641 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
     5701 gaaggtgaaa cattgagcga agaggaattt ttccgcttct gcgaacaaaa tatggcgaaa
     5761 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
     5821 ataattagaa agaatctgaa ataaatgaaa cagcagggaa ctaccctgcc agctaataat
     5881 cacgctataa aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa
     5941 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
     6001 acccttgatc gtccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
     6061 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
     6121 gagaagttct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
     6181 gctgactttg gcccgggtgg atttgcggga ttaaccgaaa ttttcaatct cgacaaaccg
     6241 gttatcgcag ctgtgaacgg ctatgccttt ggcggcggtt tcgaactggc gctggcggca
     6301 gattttattg tttgtgccga taacgccagc ttcgccctgc cggaagccaa gctgggcatc
     6361 gttcctgaca gcggcggcgt gctgcgtcta ccgaagatcc tgccgcctgc catcgtcaat
     6421 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc
     6481 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc gcagcagctg
     6541 gttaacagcg ccccgctggc gattgcggcg ctgaaagaga tcttccgcac caccagcgaa
     6601 atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccgtcg
     6661 gttctgcatt cggaggatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg
     6721 gtgtggaaag gacgttaaca accgtgagct attacgcctt tgaggggtta attccggtgg
     6781 ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag
     6841 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
     6901 aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca
     6961 tcgttgggga aaacggccat atcgggcacg gagcgatcct gcatggttgt gtgattggtc
     7021 gcgatgcatt ggtcgggatg aacagcgtga ttatggatgg cgcggtcatt ggcgaagaga
     7081 gcattgttgc cgccatgagc tttgtcaaag caggctttca cggcgagaaa cgccagttgt
     7141 tgatgggtac gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac
     7201 ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa
     7261 cacagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
     7321 cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg gataagatgc tacaacatcg
     7381 catctggcaa gcaggacgcc cggtatcaac gagataaaat taacgacgca tactctttga
     7441 caacatcatc tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg
     7501 cggcgtcttt cttacgccga ctaatttatc ccggctgtta ttgcgcaggc gcgcgtaaat
     7561 ttgctcatcg atatcgacca cttttaccag tcgctggcac tggcatcccc gtccttccag
     7621 cttattaggg atcatcttaa cttcgcagct tatttctgcg acttccgaca gaatataagt
     7681 cagggtatta accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat
     7741 cacccaccga ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac
     7801 atatccttca cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt
     7861 tgaagcaatt aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac
     7921 cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga
     7981 tttacttaat gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa
     8041 attagcggat gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc
     8101 tgcaccgaaa ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca
     8161 ccgttcaggg tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg
     8221 gagtcttcaa tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc
     8281 ttgatgcggt cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta
     8341 atgcccgctt cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt
     8401 ttcagcagtt ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc
     8461 aacgcacgac cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct
     8521 tcagcgaagg tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac
     8581 agcgggtcaa cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac
     8641 ggcgggataa cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc
     8701 gccgccactt ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc
     8761 gcacgcgggt taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc
     8821 atcagaccgc gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc
     8881 tgaatttcct gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg
     8941 cccgcctgct cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg
     9001 gcatccacgt caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg
     9061 ttagacacgc tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt
     9121 tccatcgccc gaccgccgag aacgtaagac ggacgtacca ccagtgggta gccaatctct
     9181 ttcgccttct ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt
     9241 ttcagacgct caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc
     9301 gggctggtgc cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg
     9361 gtctgaccgc cgtactgaac gataacgcct ttcggcttct caatacgcac gatttctagc
     9421 acatcttcca gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag
     9481 acggtttccg ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagcgccagc
     9541 gaggcgtgta cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg
     9601 ccgagaacca tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa
     9661 gtggagtaca tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta
     9721 taaaccgggt gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc
     9781 gccagttttg ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca
     9841 gcgttcaggc cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca
     9901 atctgtacca ggaaccagcg gtcaatattg gtcaggttga acacgccgtc cacagacagg
     9961 cccgcacgga aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg
    10021 cgacggattt tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc
    10081 gcaccgactt ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc
    10141 gccatcactt cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat
    10201 ttttcgaagt tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag
    10261 gccggagtac gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc
    10321 agtttcgccg ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa
    10381 cgggacacgc gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca
    10441 aactggacat tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg
    10501 ttacgcatga tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag
    10561 tcaccggtat ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg
    10621 tcgtttttat cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca
    10681 atcagcagct ctttggttgg ggagagatcc agaccgcggg cgcaaatttc ttcaaactct
    10741 tcgcggttat aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg
    10801 cacgggaagc ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg
    10861 gaacgcgcgg tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct
    10921 gctttatcaa tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg
    10981 ccctgacgct ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc
    11041 accgcgtccg ggcgctcttt ttcaataatc ttgcgtacta cttcccagtg aatcggctcg
    11101 atgtaggtcg catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc
    11161 agaatgacgc ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca
    11221 aactcacacg cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata
    11281 tctgtacgtt ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct
    11341 caattaactc gataaagtgg tcgaacaacg gcgctgcgtc gtgtggccca gggctggctt
    11401 ccgggtgacc ctggaagctg aacgccggtt tatcagtgcg atgaatgccc tgtaacgtac
    11461 cgtcgaacag ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg
    11521 caaaaccgtg gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat
    11581 ggttgccgcc gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca
    11641 gcagctgatg accgaggcag atgccgaata ccggaatatc ggtttcgagg aatttctgga
    11701 tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc
    11761 cgtctggatt cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc
    11821 gacagcctct atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca
    11881 cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc
    11941 agctcccttg tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc
    12001 cattcagacc tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc
    12061 ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg
    12121 tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg
    12181 tattacggaa gttgctggca atcagcggca ggtcgcgaat caccagacct tgtgcatgta
    12241 cctgagaaga ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa
    12301 gagtaacgat ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg
    12361 aagtattgaa aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa
    12421 actgggttcc gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat
    12481 attcactcac tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc
    12541 ttaccgacga atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact
    12601 taatggcggg attttttata ttattttgtg gctcatgcat gttttacagg cattaagcgg
    12661 caaaaagatc atataacctg ttctagaaaa cgcttgcgca gcaagaagat tgcatgaaat
    12721 aaaagcgagt agggaaaagt ggaccaaatg gtcaaaattt acattaaaac aacaaaaacc
    12781 gaagacaaaa catgtaattt ttggcactta taga
//
LOCUS       DAFAVF010000122        12775 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFAVF010000122 DAFAVF010000000
VERSION     DAFAVF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.122"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            41..454
                     /gene="hns"
                     /locus_tag="LGK42_003334"
     CDS             41..454
                     /gene="hns"
                     /locus_tag="LGK42_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBK2659556.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(598..1506)
                     /gene="galU"
                     /locus_tag="LGK42_003335"
     CDS             complement(598..1506)
                     /gene="galU"
                     /locus_tag="LGK42_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK2659557.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1708..2721)
                     /gene="rssB"
                     /locus_tag="LGK42_003336"
     CDS             complement(1708..2721)
                     /gene="rssB"
                     /locus_tag="LGK42_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBK2659558.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2813..3718)
                     /gene="rssA"
                     /locus_tag="LGK42_003337"
     CDS             complement(2813..3718)
                     /gene="rssA"
                     /locus_tag="LGK42_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBK2659559.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3831..4289
                     /locus_tag="LGK42_003338"
     CDS             3831..4289
                     /locus_tag="LGK42_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBK2659560.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            4339..5181
                     /gene="purU"
                     /locus_tag="LGK42_003339"
     CDS             4339..5181
                     /gene="purU"
                     /locus_tag="LGK42_003339"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBK2659561.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            5341..5425
                     /locus_tag="LGK42_003340"
     tRNA            5341..5425
                     /locus_tag="LGK42_003340"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5375..5377,aa:Tyr,seq:gta)
     gene            5460..5544
                     /locus_tag="LGK42_003341"
     tRNA            5460..5544
                     /locus_tag="LGK42_003341"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5494..5496,aa:Tyr,seq:gta)
     gene            5591..5722
                     /locus_tag="LGK42_003342"
     ncRNA           5591..5722
                     /ncRNA_class="other"
                     /locus_tag="LGK42_003342"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5906..6583)
                     /gene="narI"
                     /locus_tag="LGK42_003343"
     CDS             complement(5906..6583)
                     /gene="narI"
                     /locus_tag="LGK42_003343"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBK2659562.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6583..7293)
                     /gene="narJ"
                     /locus_tag="LGK42_003344"
     CDS             complement(6583..7293)
                     /gene="narJ"
                     /locus_tag="LGK42_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBK2659563.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(7290..8828)
                     /gene="narH"
                     /locus_tag="LGK42_003345"
     CDS             complement(7290..8828)
                     /gene="narH"
                     /locus_tag="LGK42_003345"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBK2659564.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8825..12568)
                     /locus_tag="LGK42_003346"
     CDS             complement(8825..12568)
                     /locus_tag="LGK42_003346"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBK2659565.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLTGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3086 a         3449 c         3125 g         3115 t
ORIGIN      
        1 cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat
       61 tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga
      121 agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc
      181 tgctgctgaa gttgaagagc gcactcgtaa actgcagcaa tatcgcgaaa tgctgatcgc
      241 tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat ctggcaccaa
      301 agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa
      361 aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg
      421 taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca cttgcttaaa
      481 atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct
      541 ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta
      601 cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg
      661 acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct
      721 cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc
      781 ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc
      841 cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgctttcgg
      901 tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca
      961 atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct ggctatgacc
     1021 cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata
     1081 ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca ctaccgggtg
     1141 agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt gcataatagt
     1201 tacgtgcggc ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc
     1261 cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga
     1321 gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg
     1381 aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa
     1441 catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc
     1501 agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca
     1561 tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag ttagcataat
     1621 taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat
     1681 gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc
     1741 accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata
     1801 agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt
     1861 atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata
     1921 atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta aatggtttac
     1981 ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa gctgttcctg
     2041 taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc
     2101 tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag
     2161 caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc aatgggaaat
     2221 cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc
     2281 aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat
     2341 gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt
     2401 cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcggt
     2461 ggcagatatc accagaaccg gtgtctggtc acctctgttg cgtatatgtt ccagcagttt
     2521 aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc
     2581 cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga
     2641 aaaccacgaa tccagaagcg agcgaaatac cggctcatct tcaacaatga gaatctgttt
     2701 tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat agtggcatgc
     2761 tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt
     2821 tggtgcgtac caacggcaaa agttcgtcca ttttcttttc cactgccagc tgtccggccg
     2881 caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa
     2941 tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc tccagcacct
     3001 ggatagaagt ggtcataatc tctgttgccg ttggcgctgt caccgcccga cgtgtcgtta
     3061 tgctgcctaa cctttctttc agacgcgcat gccacggcag agaatcacca ttctcgctat
     3121 tttcttcact gacattaaag gagagcaaat cttgttgcat caaatgagca tcgtgctgca
     3181 ggtcaaccgc tatcacaata tcagccccca atgcacgcgt gagggaaata ggaattgggt
     3241 taacgactgc tccatcaacc agccagtagc cgttatgtgc aacaggtgcc atgagtcctg
     3301 gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac
     3361 gtcccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt tcgatctctg
     3421 tttccggcat tatttcgcgg tattgattga agacacgctc gccgcgcagt aacccaccgc
     3481 gctgccagga gagatccatc aggcgtaaaa catcccaata actgaaagag gtcacccaat
     3541 cttccagcgc agataatcga tcgcatgcat aggcagcgcc caccagcgaa ccaattgaac
     3601 atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat
     3661 gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta
     3721 ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata
     3781 gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt gtgtctcagc
     3841 tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg
     3901 aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc
     3961 aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg gcgttacgtg
     4021 ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt
     4081 gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg
     4141 gtaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact
     4201 atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta
     4261 aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc
     4321 aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg
     4381 gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc
     4441 gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg
     4501 gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc
     4561 tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa
     4621 gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga tgtcgaaatc
     4681 gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg
     4741 tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggac
     4801 gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg ggtattaacg
     4861 ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc attcctgcct
     4921 gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa gattattggc
     4981 gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt
     5041 attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag
     5101 aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt tgtttacggt
     5161 aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg
     5221 gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat
     5281 ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc attaccccgt
     5341 ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa
     5401 ggttcgaatc cttcccccac caccatctca agtattacct caaaatttga attacccctg
     5461 gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag
     5521 gttcgaatcc ttcccccacc accatcactt tcaaaagtcc gtgaactctc aaacgaatcc
     5581 gcgattaaat attctgccca tgcggggaag gatgagaagc ctcgaccaag gttcgactcg
     5641 agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgttg
     5701 agtaatcctt cccccaccac catcacttcc ttaaataatc aaaacctgtt actccgagct
     5761 aattttgccc gatggcacca cgtttaccgg gcctacaata tcctcccctg taggtcggat
     5821 aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc
     5881 agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg
     5941 tcagatactc caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga
     6001 ataacgtcat ccccagcacc aggtgcagac ggaagataaa cgccacacca tcgaggtgtt
     6061 gagaagcgcc accgtggaag gtcaccaccg actgcgccca gccaaccagt ttcatcatct
     6121 cgctaccgtc catatgctga gcggagaacg gaatggtcaa caggcccagc gcgcactgga
     6181 taacgagcag cgacaggatc aggatatccg ctccggtagt ggttgcacgc acgcgtgggc
     6241 tgaacagacg acgcttcagc aacagcacgc cgccaatcag acacagcacg ccgctggcac
     6301 caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc
     6361 agtgcggcgt cagcataccg aagaagtgac caacaaaaat ccccagaata ccgatatgga
     6421 acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc
     6481 aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta
     6541 tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt
     6601 ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat
     6661 tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccagacagc
     6721 atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc
     6781 gctgtcgata gcggtattgg ccaatttcag cagcagatca aacatcacgg cataacggct
     6841 ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc ggcgcgatat ctttcaaacc
     6901 ttccacggct tcgctttgcg gcagctgcga caggtactcc agatacagcg gcagatggtc
     6961 cggtaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac
     7021 catcgcctgg ccgcggtcac gggattcacc gtgaacatgt tcaaatagca acagtgacgt
     7081 ggcgcggcca cggtcgaaca gttcgctgta ctgggcctgg gcatcgagcg gatccatcgc
     7141 cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatct tctttcgaca gatttttcga
     7201 cgcagcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc
     7261 aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca
     7321 tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag
     7381 ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca
     7441 cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac
     7501 atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta
     7561 ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagtgcgcgc
     7621 agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc
     7681 aagctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgccgcagac
     7741 tgaatcggag acagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc
     7801 agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcggct
     7861 tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc
     7921 ggatcgagga acacctccag ctgacgctgg taaagatctt tctcgttctc ggtgctggct
     7981 gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca
     8041 caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac
     8101 ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca tccggtgatg
     8161 cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc
     8221 ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac
     8281 aggcgcggca aatacatcat aaaggtgttt tcgaactggc tatacatcgc cttctggatg
     8341 ttgtcgaagt tcttgtcttt cgccagcttg tcaaactcac cgcccaggtc atcttcccag
     8401 ttcggccctt tttcgatttt cgccatccgt tcgccggtaa tcagcgaacg cggacgggca
     8461 atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac
     8521 ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc
     8581 atggcacggt tacccatgcg cggctgcagt ttgccgttga ttttacggat ccagccgcct
     8641 ttgtattttt cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg
     8701 ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact
     8761 gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga
     8821 attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac cagtcaatgt
     8881 tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt
     8941 taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac
     9001 gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa
     9061 cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa
     9121 ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga
     9181 tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag
     9241 tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca
     9301 ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg
     9361 aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact
     9421 ggtgatcctg atacagttgc tgacgaccag tgagcgtacg ccatgggatc agctcgtgaa
     9481 cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc
     9541 tggagataat tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt
     9601 tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt
     9661 tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa
     9721 ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct
     9781 tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccattaccga
     9841 ttttctccat cagcgggccg atagaggtaa agcgttcgta agtcgccgga taatcacgct
     9901 ctacgaccat aatgtgtggc gcggttttac ctgggatcag gtcgcactcg ccttttttcc
     9961 agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcatgctgg ataggcagcg
    10021 tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga
    10081 tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca
    10141 gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg
    10201 ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa
    10261 ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca
    10321 cgccgccctg ttgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact
    10381 tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagatgaaca
    10441 ggttgcgcgg gtggttttta ccgttttctg gttgttctgc cgcaaaacgg atggaaccct
    10501 ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttcc gcttcgccag
    10561 cgatagtcag cgggttagtg cctaactgcg gtgcagacgg cagccagccc atgcgttccg
    10621 cacggacgtt aaagtcgatc aagtgtccgg tatagcggga tttgtccgcc atcggtgaca
    10681 gcaactcttc cgccgtgacg gtttcataac gccactggct ggagtggtta tagaaataag
    10741 aagtgctgtt catgtgacgc gccggacgct gccagtcaag ggcaaacgcc agcggctgcc
    10801 agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct
    10861 gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt
    10921 gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag
    10981 cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct
    11041 gctcggccca ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt
    11101 cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca
    11161 gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt
    11221 tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta
    11281 cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt tcttcgccag
    11341 ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag
    11401 aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat
    11461 tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt
    11521 agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat
    11581 agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt acgtggccca
    11641 tcgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt
    11701 tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtccctttg taacgcactt
    11761 cagtaaagaa gtgagcatcc ggggtacgcg tctgcggcac gtttgacccc caggcgatga
    11821 tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt
    11881 gcggagacgc aggaggcaag tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa
    11941 tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg
    12001 agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga
    12061 tcagttcgtt cacctcctgc caggaagaac gaacaaatcc accgcgtcca cgcgcctgct
    12121 taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc
    12181 tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt
    12241 tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag
    12301 gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg
    12361 tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt
    12421 gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac
    12481 ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt
    12541 agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc
    12601 tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat
    12661 tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta
    12721 ggataattaa cagacaccac ataacagact gtaaattaag acgttttata cctgt
//
LOCUS       DAFAVF010000123        12761 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFAVF010000123 DAFAVF010000000
VERSION     DAFAVF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.123"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(157..1248)
                     /gene="ychF"
                     /locus_tag="LGK42_003347"
     CDS             complement(157..1248)
                     /gene="ychF"
                     /locus_tag="LGK42_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK2659566.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1365..1949)
                     /gene="pth"
                     /locus_tag="LGK42_003348"
     CDS             complement(1365..1949)
                     /gene="pth"
                     /locus_tag="LGK42_003348"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK2659567.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2227..2505
                     /gene="ychH"
                     /locus_tag="LGK42_003349"
     CDS             2227..2505
                     /gene="ychH"
                     /locus_tag="LGK42_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBK2659568.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2560..4239)
                     /gene="dauA"
                     /locus_tag="LGK42_003350"
     CDS             complement(2560..4239)
                     /gene="dauA"
                     /locus_tag="LGK42_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBK2659569.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVMAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4364..5311)
                     /gene="prs"
                     /locus_tag="LGK42_003351"
     CDS             complement(4364..5311)
                     /gene="prs"
                     /locus_tag="LGK42_003351"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK2659570.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5462..6313)
                     /gene="ispE"
                     /locus_tag="LGK42_003352"
     CDS             complement(5462..6313)
                     /gene="ispE"
                     /locus_tag="LGK42_003352"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK2659571.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6313..6936)
                     /gene="lolB"
                     /locus_tag="LGK42_003353"
     CDS             complement(6313..6936)
                     /gene="lolB"
                     /locus_tag="LGK42_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBK2659572.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7150..8406
                     /gene="hemA"
                     /locus_tag="LGK42_003354"
     CDS             7150..8406
                     /gene="hemA"
                     /locus_tag="LGK42_003354"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBK2659573.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8448..9530
                     /gene="prfA"
                     /locus_tag="LGK42_003355"
     CDS             8448..9530
                     /gene="prfA"
                     /locus_tag="LGK42_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK2659574.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9530..10363
                     /gene="prmC"
                     /locus_tag="LGK42_003356"
     CDS             9530..10363
                     /gene="prmC"
                     /locus_tag="LGK42_003356"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK2659575.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10360..10752
                     /locus_tag="LGK42_003357"
     CDS             10360..10752
                     /locus_tag="LGK42_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBK2659576.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10756..11565
                     /locus_tag="LGK42_003358"
     CDS             10756..11565
                     /locus_tag="LGK42_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBK2659577.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11601..12455
                     /gene="kdsA"
                     /locus_tag="LGK42_003359"
     CDS             11601..12455
                     /gene="kdsA"
                     /locus_tag="LGK42_003359"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBK2659578.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12604..12711)
                     /locus_tag="LGK42_003360"
     CDS             complement(12604..12711)
                     /locus_tag="LGK42_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBK2659579.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3174 a         3242 c         3321 g         3024 t
ORIGIN      
        1 tctcgtggtt cactgtaggc aataagcaca aaagtgtagg atgttacaag aatgattagg
       61 actcggtgaa atgaaaaatc cacgcaattg cgtggattat atatactttt gcgctcttca
      121 tgagatttag cgaaacctca tgagacaaca aattaattag acgttgaaaa ggaagttcat
      181 cacatcgcca tctttaacga tgtaatcttt accttctgca cgcattttgc ctgcttcttt
      241 cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac gagatggttt gtgcacggat
      301 aaagcctttt tcaaaatcag tatggatttt gcccgctgcc tgcggcgcgg ttgctccaac
      361 cggaatggtc catgcacgca cttctttcac cccagcggtg aagtaagttt gcaggttcag
      421 cagcttataa ccggcacgga tcacacggtt caggcccggc tcttccagcc caagctcctg
      481 cataaactcg tcacgttctt cgtcgtccag ttcggcaatg tctgcttcaa cagcagcaca
      541 aaccggaacc acaacagaac cttctttcgc cgcgatttca cgcacctggt caagatatgg
      601 gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac attgttggtt tcagcgtcag
      661 gaagctcagg taacgaatag ccgctttctc ttcagcgctt aaatccagcg cgcgcagcat
      721 acctgcattt tccaactggg gcaggcattt ttccaggacc gccagctcag ctttcgcgtc
      781 tttatcgcca cctttggctt tcttctgtac gcgatgaatc gcacgttcgc aggtgtcgag
      841 gtctgccagc gccagttcgg tgttgataac ttcaatatcg tcagccgggt taactttgcc
      901 ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca acgtgaccga tcgcttcggt
      961 ttcacggatg ttggtcagga actggttacc cagaccttcg cctttcgatg cgccttttac
     1021 cagaccggcg atatcgacaa attccatggt cgtgggaagc gtacgttgcg gttttacgat
     1081 ttcagccagt tgatccaggc gagggtcagg cattggtacg acgcctgtgt tcggctcaat
     1141 ggtgcagaat ggaaagttgg ccgcttcaat accggctttg gtcagcgcgt tgaacagggt
     1201 agatttcccg acgttgggca aaccgacgat accgcatttg aatcccatga tttaaatcac
     1261 cttaatatct taataatcaa cctgttattg ctaacagatt gcagaaatgg aaataacttt
     1321 gcctattata cacggcactc ggcaaaaatg ccgcagacaa cgacttattg cgctttaaag
     1381 gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa accacatttc agtacaacgc
     1441 gccgcttcgt cgatggcttc atcaattaac ttctgttcac taacaggcgg tttgcctaac
     1501 acaaaaccga caactttatt tttatcgccc ggatgaccga ttccgatgcg taaacggtga
     1561 aagttagggt tattacccaa tttactgatg atgtctttca gtccattgtg accaccatgg
     1621 ccaccgccca atttaaattt ggcgacgcca ggaggcagat ccagttcgtc gtgggccacc
     1681 agaatttcgt ccggattaat gcggaaaaaa ctggccatcg ccgcaacggc tttgccgctg
     1741 agattcataa atgtagtcgg gactaacagg cggacatctt cgcctccaag agtgactcgc
     1801 gaagtataac caaagaattt agcctcttcg cgcagcggag cgcgcaaacg ctctgccagt
     1861 aagtcaacga accaggcacc agcattatgt cgcgttgcgg cgtattcagc accggggttc
     1921 gccaggccga caatcaattt aatcgtcacg tttttttgtc ctgagtgtgt acataactgg
     1981 cgcgtagttt actggttgcg gccccgcttg acaaaaaact gcgtatcaaa tgcagataac
     2041 gtaataattg catgagtgga ctattagaaa gtcaaggtgt tcaggcgttt atttgtaaag
     2101 ttttgttgaa ataagggttg taattgtgat cacgcccgca cataacccac agggtgttgt
     2161 ctatacttta cacataagga agaggggtat tccctgttac aacccagaaa gttccggagg
     2221 tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg tgctcatggg gttgggtctg
     2281 gtggtaatgg tggtcggcgt ggggtattca atcctcaacc agttaccaca gtttaatatg
     2341 ccccagtatt tcgcacatgg tgcagtgcta agtattttcg tcggtgccat tctctggctg
     2401 gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc gttactggtg ggttcgccac
     2461 tatgacaaac gttgccgccg tagcgataat cgccgtcata gctaacaaat aatgcagttt
     2521 gctgaccagt cagtttgcgc tgactggtca atctcgtact tataaatccg ccatcgccgc
     2581 gcgacgattc gggaagaacg ccaggcgtcc cgggatcggt tgaatgccag cgcgagccat
     2641 agtgcgcagt ggctggaatt ccacgttgca cacgcgcagt tcacatccct cgggcagacg
     2701 cttcacaaaa cgctggaacg catcaagacc accagcatca agtaccggaa ccgcatccca
     2761 cttcagaatc acaatccgtt tgccttcaag acgtgactcc aggtccgtga acaagccttc
     2821 agcagcagca aaaaacagcg ggccaataac gcgcagaacc aggacatcgt ctggaacatc
     2881 tacgactacc ggtgccaggc gagtcatacg tgcgatacga cgcataaaca gcagcgatgc
     2941 cagcacgatc cccacgctga tggcaataac catatcaaac agcacggtca gcgacatgca
     3001 cagcagcatg acaatgatgt catctttcgg cgcatgacgc agcaagtcga ccactttgtg
     3061 cgcttcactc atgttccacg ccaccatcaa cagcagggca gccatggcgg aaagcggcag
     3121 ccaggagagc agcggtgcca ttaccagcag ggcaagaata accagaatag agtggatcac
     3181 cgccgagata ggggaagttg ccccggcacg gacgttagcg gcagaacgcg cgatggcagc
     3241 tgtagcggta atgccaccaa agaacggagc gatgatattc cccagtccct gtccaaccag
     3301 ttcgctattc gccttgtgtt tcgtcccggt cataccatcc agcaccacgg cgcagagcag
     3361 agattcgatt gcgccgagca ttgccattga gaatgccgca ggcagcagtg tgcgaataga
     3421 atcccaggtt agcgtgaatt ctgaattagg cagatcccac ggcagcacca gttgcggcag
     3481 cagttgcgga ataccgttac cctgagaacc atcggccagg acgtagtgga attgcgaacc
     3541 gatggtagca acatgtccgc cgagcaggtt aacaatcccc atcaccgcgc aaccagccag
     3601 caaggccgga aggtgaccgg gtaaacgaat gcccagacgc ggccagaaaa caagaatacc
     3661 tagcgtcaca atgccaatgg cagcatcacc cacattaatg gtcggcagcg ccataaataa
     3721 tgcgccgact ttttgtagat aatgttccgg gacatgggcc atttgcagac cgagaaaatc
     3781 tttaatctgc atggtaccga tggtgatccc gatacccgag gtgaaaccta aggtgacgga
     3841 aaccggaata tactcaatca ggcgaccaaa gcgtgccaga cccatcagaa tcaaaaaaat
     3901 ccccgacagc aaggtcgcaa ccagcagtcc tgccagtcca aactgttgcg acacgggata
     3961 gagaattacc acaaatgccg cagtcggacc ggaaacgcta aagcgtgacc cacccgtcag
     4021 agcaatgaca atccccgcaa cagctgcggt atataaaccg tactggggtg ccacaccact
     4081 accaatagcc aacgccatcg ccagcgggat agcaataatc ccgacggtta tcccggcaat
     4141 caggtcacgg gtaaaccgtg cggcagtata tttttctttc cagcaagcgt cgatcagagc
     4201 gcggaaaggc atcacatgtg aggaaaatat tttgttcaca ataatgtttc atccgtgagc
     4261 gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag gtcatacaaa tggatattac
     4321 agacaaaaaa acccgccgca gcgggtcttt gagccgggtt cgattagtgt tcgaacatgg
     4381 cagagatcga ttcttcgttg ctgatacgac gaatcgcttc ggccagcata cctgacaggg
     4441 tcagagtacg cacgttcggc agtgatttga tttcatcgct cagcggaatg gtatcgcaga
     4501 caacgacttc atcaattaca gagttacgca ggttgttcgc cgcgttgcca gagaagatcg
     4561 ggtgagtcgc gtacgcaaat acacgtttag caccacgttc tttcagagct tcagcagctt
     4621 tacacagcgt accgccagtg tcgatcatat catcgaccag tacgcagtca cgacctgcaa
     4681 cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg cggacgacgt ttgtcgatga
     4741 ttgccatatc ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca acgccgccga
     4801 tgtccggaga aaccacaatt gggttatcca gattcagctg cagcatgtct tccagcagga
     4861 tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc tgttcagcgt
     4921 gcagatccac tgtcagcaca cggtcaacac cgacgctgga gaggaagtct gcaaccactt
     4981 tcgcagtgat tggtacacga gcggaacgga cgcgacggtc ctggcgcgca tagccaaagt
     5041 aggggataac agcggtgata cgacctgcgg aagcacgacg cagggcatca accataacga
     5101 ctaattccat caggttgtcg ttagtagggg cacaagtgga ctggatgatg aaaatatcac
     5161 caccgcgtac attttcatta atttgtacgc tgacttcgcc atcgctaaag cgacctacag
     5221 cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt tccggggtgg
     5281 cgttaccagc aaaaagcttc atatcaggca cgagaagaac ctcaggcatg cgtccattgg
     5341 tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat cctttccagg cggtgtatta
     5401 aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca gcttgcccgg
     5461 cttaaagcat ggctctgtgc aatggggaaa gattaacgcc tttcgccaca aagccattga
     5521 gccattccgg ggcttgctct agcacctggc gggcttcaga ctctgtatca aattcagcaa
     5581 agacacaggc ccctgtccca gtcaggcgcg acggggcgta ttctaacagc caggaaagca
     5641 ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc gcaatcattg ctgaattcac
     5701 attttagcaa cgtttctatt gacctttttg gcgtattgcg cgggagttca ggatctttaa
     5761 aaatcaccgg agtcggaata cttacaccag ggtgcgccac cagataccac ttctctggcg
     5821 gatccaccgg cgttagtatt tcaccaacgc cttcggcaaa cgcggcatgc ccccgaacaa
     5881 agacaggaac atctgcgccc agcgtcagcc ccatttccgc cagctcatcc atgcttagcc
     5941 cgcattgcca gagatgattt aatgccacca ggaccgtcgc ggcattggat gaaccaccgc
     6001 cgagaccgcc gcccatcggc aaacgcttgt caatgctgat attcgcaccg cttcccgtcg
     6061 gaagacgccc gctgtctgcc gcagttttca tcaacaatcg cgctgcgcga acgatcaggt
     6121 tatcttcatg ttccacgcct tcaacgggcg ttaacagacg aatatcccca tcgtcacgaa
     6181 gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc agcgtgtggt
     6241 aaccatccgc acgctgaccg gtaatgtata aaaacagatt aagttttgcc ggagagggcc
     6301 actgtgtccg cattatttca ctatccagtt atccattttt aacttgatgc gttgaccacc
     6361 gtcggtgagt tccatattgg ctggcatcgc aggttgcgtt ttggtgtcat aaccaccata
     6421 aacaaccttc cagtttttgc cattctggct gtaggtaatt tcgctcaggc ggtactggtc
     6481 gtccagtttg tagtcggttg catcacccgg taaacctaaa atccactggc gcaagctgtt
     6541 gagcggaatt ggcattccgg tcaatttgcc aatcatctct tcggcgtcat cggcggtata
     6601 acgctgacct ttattgtcga ctaactgcac gttacccggt tgagcattca gctccagttc
     6661 cgtgctgccc aatgggttag tgagcagcag acggtagcga tcctggccgg tttgctgcca
     6721 gaaaaagcgg gcgtacactt tttgttggtc agaaatataa gcgaacgcgc cgcgagtctg
     6781 atactgatta agattgcgca cgtcttgctg atgctgacgc cattgtggcg aatccgggct
     6841 tttgccagga cctttgggcg tggtaacgga acaggcagtg agcacaagag cagccagcgg
     6901 tagcaggcgg ataagacgaa aatcgggcag gggcatagtg atgacaagtc cttgagatac
     6961 gttgcagtta taacccttaa tgctagcgtt accgtccgct atcgtctatg ttcaagttgt
     7021 cttaattgcc agaatctaac ggctttcggc aattactcca aaagggggcg ctctctttta
     7081 ttgatcttac gcatcctgta tgatgcaagc agactaaccc tatcaacgtt ggtattattt
     7141 cccgcagaca tgaccctttt agcactcggt atcaaccata aaacggcacc tgtatcgctg
     7201 cgagaacgtg tatcgttttc gccggataag ctcgatcagg cgcttgacag cctgcttgcg
     7261 cagccgatgg tgcagggcgg cgtggtgctg tcgacgtgca accgcacgga actttatctt
     7321 agcgttgaag agcaggataa cctgcaagag gcgttaatcc gctggctttg cgattatcac
     7381 aatcttaatg aagaagatct gcgtaaaagc ctctactggc atcaggataa cgacgcggtt
     7441 agccatttaa tgcgtgttgc cagcggcctg gattcactgg ttcttgggga gccgcagatc
     7501 ctcggtcagg ttaaaaaagc gtttgccgat tcgcaaaaag gtcatatgaa ggccagcgaa
     7561 ctggaacgca tgttccagaa atctttctct gtagcgaaac gcgttcgcac tgaaacagat
     7621 atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg gcagatcttt
     7681 gaatcgctct ctacggtcac agtgttgctg gtaggcgcgg gcgaaaccat cgagctggta
     7741 gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta tcgccaaccg cactcgcgaa
     7801 cgtgcccaaa ttctggcaga tgaagttggc gcggaagtga ttgccctgag tgagatcgac
     7861 gaacgtctgc gcgaagccga tatcatcatc agttccaccg ccagcccgtt accgattatc
     7921 gggaaaggca tggtggagcg cgcattaaaa agtcgtcgta accaaccaat gctgttggtg
     7981 gatattgccg ttccgcgcga tgttgagccg gaagttggca aactggcgaa tgcttatctt
     8041 tatagtgtgg acgatctgca aagcataatt tcgcacaacc tggcgcagcg taaagccgca
     8101 gcggttgagg cggaaactat tgtcgctcag gaaaccagcg aatttatggc ttggctgcga
     8161 gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc aggcagagca cgttcgcgat
     8221 gagttaaccg ccaaagcgtt agcggccctt gagcagggcg gcgacgcgca agccattatg
     8281 caggatctgg catggaaact gactaaccgc ttgatccatg cgccaacgaa atcacttcaa
     8341 caggccgccc gtgacgggga taacgaacgc ctgaatattc tgcgcgacag cctcgggctg
     8401 gagtagcagt acatcatttt ctttttttac agggtgcatt tacgcctatg aagccttcta
     8461 tcgttgccaa actggaagcc ctgcatgaac gccatgaaga agttcaggcg ttgctgggtg
     8521 acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt atcacgcgaa tatgcgcagt
     8581 taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat atcgaaaccg
     8641 cacagatgat gctcgatgat cctgaaatgc gtgagatggc gcaggatgaa ctgcgcgaag
     8701 ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt tctgttactg ccaaaagatc
     8761 ctgatgacga acgtaacgcc ttcctcgaag tccgagccgg aaccggcggc gacgaagcgg
     8821 cgctgttcgc aggcgatctg ttccgtatgt acagccgtta cgccgaagcc cgccgctggc
     8881 gggtagaaat catgagcgcc agcgagggtg aacatggtgg ttataaagag atcatcgcca
     8941 aaattagcgg tgatggtgtg tatggtcgtc tgaaatttga atctggcggt catcgcgtgc
     9001 agcgtgttcc tgctacggaa tcgcagggtc gtattcatac ttctgcttgt accgttgcgg
     9061 taatgccaga actgcctgac gcagaactgc cggacatcaa cccagcagat ttacgcattg
     9121 atactttccg ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat tcggcaattc
     9181 gtattactca cttgccgacc gggattgttg ttgaatgtca ggatgaacgt tcacaacata
     9241 aaaacaaagc gaaggcgctt tcagtgctcg gtgctcgcat ccacgctgct gaaatggcaa
     9301 aacgccaaca ggccgaagcg tctacccgtc gtaacctgct ggggagtggc gatcgcagcg
     9361 accgtaaccg tacttacaat ttcccgcagg ggcgcgttac cgatcaccgc atcaacctga
     9421 cgctctaccg cctggatgaa gtgatggaag gtaagctgga tatgctgatt gaaccgatta
     9481 tccaggaaca tcaggccgac caactggcgg cgttgtccga gcaggaataa tggaatatca
     9541 acactggtta cgtgaagcaa taagccaact tcaggcgagc gaaagcccgc ggcgtgatgc
     9601 tgaaatcctg ctggagcatg ttaccggcag agggcgtact tttattctcg cctttggtga
     9661 aacgcagctg actgacgaac aatgtcagca acttgatgcg ctactgacac gtcgtcgcga
     9721 tggtgaaccc attgctcatt taaccggggt gcgagaattc tggtcgttgc cgttatttgt
     9781 ttcgccagcg accttaattc cgcgcccgga tacggagtgt ctggtggagc aggcactggc
     9841 gcggttgcct gaacaacctt gccgtattct tgatctcggg acgggtaccg gggcgattgc
     9901 gcttgcgctg gctagcgagc gcccggactg cgaaattatc gctgtagatc gtatgcctga
     9961 tgctgtctcc ctggcacaac gtaatgccca gcatctggcg atcaaaaata tccacattct
    10021 gcaaagcgac tggtttagcg cgctagccgg gcagcagttt gcgatgattg tcagcaatcc
    10081 gccgtatatt gacgagcagg acccacatct tcaacaaggc gatgtccgct ttgagccgct
    10141 cactgcgctg gttgcggcag acagtggaat ggcagacatc gtgcatatca tcgaacagtc
    10201 gcgtaacgcg ctggtatccg gcggctttct gcttctggaa catggctggc agcagggcga
    10261 agcggtgcga caagcattta tcctcgcggg gtatcatgac gtcgaaacct gccgtgacta
    10321 tggtgataac gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt ttctacactg
    10381 cttagtgttc atcttattag tctcgcgctt tctgttgggc tattaacctt acgtttctgg
    10441 ctacgttatc agaagcatcc acaggcattt gctcgctgga cgcgcattgt gccgccggtt
    10501 gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca catcctgcca
    10561 ttttccgggc aggcacagtg gctgactgaa aagctgtttg gagttatcat ttatatcgtt
    10621 ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc agcaggcgcg cattattgcc
    10681 ttcccgctgg cgttggtggt gctgtacatc atcattaaac tcgccaccac aaaagtaccg
    10741 ttactggggt aagtcatgag atcgttagct gatttcgaat ttaataaagc gccattgtgc
    10801 gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca agatgtttac
    10861 gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa tcagccagct tctgccttta
    10921 gaagagcagc tggaaaaact gatcgcgctg ttttacggcg actggggatt taaagcctca
    10981 cgcggcgttt atcgtctttc cgatgcatta tggctggacc aggtgttaaa gaatcgacag
    11041 ggcagtgcgg tatcattagg tgcggtttta ttatgggtcg cgaatcgtct cgatttgccg
    11101 ctgctgccgg tgattttccc tacgcagctg atattacgca ttgaatgtcc ggatggcgaa
    11161 atttggctga ttaatccttt taacggtgaa tcgttaagcg aacatatgct ggacgtatgg
    11221 ttaaagggaa atatcagccc gtcggcggaa ctgttttatg aagaccttga tgaagctgat
    11281 aacattgagg taatccgcaa attgctggat acactcaaag cctcgttgat ggaagaaaat
    11341 cagatggagc tggcgttacg caccagcgaa gctttattac aattcaaccc tgaagatccc
    11401 tatgaaattc gcgatcgcgg gttgatttat gcgcaactgg attgcgaaca cgttgcgttg
    11461 aacgatttaa gttatttcgt tgaacagtgt ccggaagacc cgatcagcga aatgatccgt
    11521 gcgcaaataa ataacatcgc gcataaacat attgtgctgc attaattaat cgacatttta
    11581 ctcaagatta aggcgatcct atgaaacaaa aagtggttag cattggcgac atcaacgtag
    11641 caaatgacct gccgttcgta ctgtttggcg gtatgaacgt gctggaatct cgcgatctgg
    11701 cgatgcgcat ttgcgagcac tacgtaactg tgacccagaa actgggtatc ccttacgtgt
    11761 tcaaagcctc ttttgacaaa gccaaccgct cctccatcca ctcttatcgt ggaccgggcc
    11821 tggaagaagg gatgaaaatc ttccaggagt tgaagcagac ttttggcgtg aaaattatca
    11881 ccgacgttca cgaaccaagt caggcacagc ccgttgctga tgtcgtggat gtgattcagt
    11941 tgccggcgtt tcttgctcgc cagactgacc tggttgaagc catggcgaaa accggtgcgg
    12001 taattaacgt caagaaacca cagtttgtca gcccgggaca gatgggtaat atcgttgata
    12061 aattcaaaga aggcggcaac gaaaaagtga ttctttgcga tcgtggtgct aacttcggct
    12121 atgacaacct ggttgtcgat atgctgggct tcagcattat gaagaaagtg tctggtaact
    12181 cgccggtgat tttcgacgtg acccacgcac tgcaatgccg cgatccgttt ggcgcagctt
    12241 ccggtggtcg tcgtgctcag gtggctgagc tggcacgagc cggtatggcg gtaggtctgg
    12301 cggggctgtt tattgaagcg catccggatc cggaacatgc gaaatgtgat ggtccatccg
    12361 cgctgccgct ggctaaactg gaaccgttcc tcaagcagat gaaagcgatt gatgatctgg
    12421 tgaaaggttt cgaagaactg gataccagca agtaatcttt tttgcttgaa aaataaagtc
    12481 ttagcgttct gcgttaagac ttttttcatg ggtgccagat acaaaaaagg ccgcaggctg
    12541 ttacccctgc ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc
    12601 accttacttc cggttacgcc accagccgac aatcgctgcg gtaataattc ccgccaggat
    12661 cggtgctgcc aggtcgtgcc agaaaatcat ggcaaactgc gcgagcgtca tatagccgcc
    12721 ttgttgtaat gacaacattt tgcggctatt cttgaattgt t
//
LOCUS       DAFAVF010000124        12395 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFAVF010000124 DAFAVF010000000
VERSION     DAFAVF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.124"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(78..851)
                     /gene="uxuR"
                     /locus_tag="LGK42_003361"
     CDS             complement(78..851)
                     /gene="uxuR"
                     /locus_tag="LGK42_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBK2659580.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1066..2526)
                     /locus_tag="LGK42_003362"
     CDS             complement(1066..2526)
                     /locus_tag="LGK42_003362"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBK2659581.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2607..3791)
                     /gene="uxuA"
                     /locus_tag="LGK42_003363"
     CDS             complement(2607..3791)
                     /gene="uxuA"
                     /locus_tag="LGK42_003363"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK2659582.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4131..5474
                     /gene="gntP"
                     /locus_tag="LGK42_003364"
     CDS             4131..5474
                     /gene="gntP"
                     /locus_tag="LGK42_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBK2659583.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5647..6549)
                     /gene="fimH"
                     /locus_tag="LGK42_003365"
     CDS             complement(5647..6549)
                     /gene="fimH"
                     /locus_tag="LGK42_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBK2659584.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(6569..7072)
                     /gene="fimG"
                     /locus_tag="LGK42_003366"
     CDS             complement(6569..7072)
                     /gene="fimG"
                     /locus_tag="LGK42_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBK2659585.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7085..7615)
                     /gene="fimF"
                     /locus_tag="LGK42_003367"
     CDS             complement(7085..7615)
                     /gene="fimF"
                     /locus_tag="LGK42_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBK2659586.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAVAGLGIQLLNEQQNQIPINAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7625..10261)
                     /gene="fimD"
                     /locus_tag="LGK42_003368"
     CDS             complement(7625..10261)
                     /gene="fimD"
                     /locus_tag="LGK42_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBK2659587.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMELNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLVGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10328..11053)
                     /gene="fimC"
                     /locus_tag="LGK42_003369"
     CDS             complement(10328..11053)
                     /gene="fimC"
                     /locus_tag="LGK42_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBK2659588.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11090..11629)
                     /locus_tag="LGK42_003370"
     CDS             complement(11090..11629)
                     /locus_tag="LGK42_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2659589.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11694..12248)
                     /gene="fimA"
                     /locus_tag="LGK42_003371"
     CDS             complement(11694..12248)
                     /gene="fimA"
                     /locus_tag="LGK42_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2659590.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         2920 a         3285 c         3030 g         3160 t
ORIGIN      
        1 tacaaagcaa tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt
       61 gcgcttatta taataagtca gacgtcgact ttatccagcg gccatgaatc aaacagatag
      121 ccgtcaaaat aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc
      181 agatgctgcc acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg
      241 atctgtttgt gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc
      301 aactggatcc acattggatt gttttcccgc cactgccagg actgacggaa taactccacc
      361 agcatgctgt tatgcgttgc ttcggcaatg gcgagatgga actgcatatc accgctttcg
      421 ctgctacccg gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt
      481 ttgacgatat cttcgcgggt agcctgcaaa gcggcaaatt ccgcgatgtt actttccagt
      541 aactgacgcg cctgtaacag ctcaaaagga cccgcatcat tacagacgtt ggcatccgaa
      601 ctgtctgtgt tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact
      661 tccaccagcc ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca
      721 tcaagcattt ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc
      781 tttatgatca gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg
      841 gcagatttca tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc
      901 gtgaacgcct tatccggcct acaacgggca attttacggc ccctgttaat ctcaaactat
      961 accattagca ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca
     1021 aatatcagtt tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac
     1081 acactcgcgc gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa
     1141 gtcggcattc tgcggcagat catcggcaaa aataccgctc aggcctagca gcgctttcac
     1201 gcggtctgcg ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac
     1261 cacgtcaatg gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac
     1321 gcccagcgcc aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag
     1381 cagacgctgc ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag
     1441 agacgggttg ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc
     1501 cggcatcgac agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata
     1561 atccgggtta gtcatggtgt cggcaatagt ttcatagccg ccgaggtaac cgaggtacgc
     1621 caggaaagag tggctaccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc
     1681 tgcaacgaac tgtgcgccca ctttatccca gtccgggcga ccattaacga aattatcttc
     1741 aatcacccac tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cgcccagctg
     1801 gtcagcaatt tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt
     1861 gcacgggaag gtgacgttct cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc
     1921 cagccccagt accgcgacct tcgccacatg accgttttca cgcacgttat cgcaggacat
     1981 caccgtaaat gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc
     2041 aatcgcggac ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag
     2101 atccagttga ccgcttgccg catcagcgca gtagcctttt tccgtgaccg tcagagagac
     2161 aatcgccgtt tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg
     2221 gtgcagcgct tctttcatcg aaccgataat tttcagctcg gtgctttccg cgcctttttc
     2281 cgccacggtg tacagcagtt gctgtttttt caggttttcg atcagcacgc ggtcgttgcc
     2341 tggcatcagg ttaacttcgc agatacccca gtcgctgtcg gtgctttcca gcagatgatg
     2401 ggtgtacagc gcctggtgcg cacggtgaaa cgccccgcaa ccgagatgca caatgcgtga
     2461 ttccagacga gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt
     2521 agtcatcgta aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc
     2581 acggcgcgca gccatgccgg tggatattaa cggctaaaga aagcgcgctg gatcgccagt
     2641 tcgacaccgc gaacttcggc caggcctttc agacgaccaa ttgcggagta acctgggttg
     2701 gttttcttct tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg
     2761 tcttctttgc cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg
     2821 tacatatcaa cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca
     2881 cgcatggtgg agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca
     2941 accagatcgt tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc
     3001 atgctattta cggtatcaac catccactgc atatcttcaa tggtagaaac aatgcgcggc
     3061 aggccgagga ttgggcgcgg cggatcgtcc gggtgaacag ccatacgcac gccaacttct
     3121 tcggcaaccg gaataatcgc cttcaggaag acggcaaagt tttcgcgcag tttggctttg
     3181 tcgatatctt tgtacagctc caggtgctta cggaactggt cgagggtgta cccttcttcc
     3241 gcgcccggca gaccggcaat gatgttacgg gtcagacgcg ctttgtcttc gtcgctcata
     3301 gtggcgaagc gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcaccc
     3361 gggcgtttca ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct
     3421 ttggagccgt ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg
     3481 aagttgtagc acacggtgcg aataccgcac tgcgccaggt tgcgcagagt ctgctgatag
     3541 ttagcgatcc actgctcata gttgccagtg tgggttttga tatcttcgtg aattggcacg
     3601 ctttctacga cagaccacac caggcctgca tcttcaacga tagccttgcg tttgaggatc
     3661 tcttctacgg accatacttc accgttcggg atatggtgca aagcggtaac cacgccagtc
     3721 gcgcccgcct gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag
     3781 gtctgttcca tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac
     3841 cctggagttg tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga
     3901 tgagatggcg tataagttct accttttggt aagacatgtc aacccgaatt ctgaaattgg
     3961 ttaaccacat cacaagaatt tcactctcct ggttgttgct gcaaattgca gcaaaaggta
     4021 atcgatggtt attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca
     4081 taacatgtcg cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc
     4141 ttaacattct ctgggtggta ttcggcattg gtctgatgct ggtactgaat ttgaagttca
     4201 aaatcaattc aatggtggcc ttgttggtgg cggcgctgtc cgtcgggatg ctggcgggca
     4261 tggatttgat gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggggaac
     4321 tggctatcat cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg
     4381 ctcaccagat agcgcatacg ctgctggcac gtctcggtct gcgttatgta cagctgtcgg
     4441 tgattatcat cggcctgatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt
     4501 tagcaccgct ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga
     4561 tcccggcagt agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg
     4621 tggcgctggt gaatgcttat ggcgcggata tggggatggt ttatatctat ggcgtactgg
     4681 tgacgatccc aagtgtcatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg
     4741 agcgcccaac gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct
     4801 ctttcggcgt ttcgattctg gtgccgttga tcccggcgat cattatgatc tctaccacca
     4861 tcgccaatat ctggctggta aaagataccc ctgcgtggga agtggttaac tttatcggtt
     4921 cctcgccgat tgcaatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc
     4981 gcgggcacga tatgcagtgg gtaatgaacg cttttgaaag cgcggtgaaa agcattgcaa
     5041 tggtgattct gatcatcggt gcaggtggcg tgctgaagca gaccatcatc gacaccggca
     5101 tcggcgacac catcggcatg ctgatgtccc acggcaatat ctcgccctac atcatggcat
     5161 ggctgatcac cgtactgatt cgtctggcga cgggtcaggg tgtggtttca gcgatgaccg
     5221 ccgccgggat tatcagtgca gcaatccttg atccagcaac tggtcagctg gttggcgtga
     5281 atccggcgct gctggtactg gcgacggctg cgggttccaa caccctcacc cacattaacg
     5341 atgcctcatt ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa
     5401 cctggggtct gctggagctg gtcaactccg tggttgggct gattattgtg ctgattatta
     5461 gcatggtagc gtaaaagagc acctgagcct gccattggca ggctctgttc ggattgtcgg
     5521 taaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg
     5581 gctaacgtgc aggttttagc ttcaggtaat attgcgtacc tgcattagca atgccctgtg
     5641 atttctttat tgataaacaa aagtcacggc aataatcgat tgcacattcc ctgcagtcac
     5701 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagtccctac
     5761 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     5821 gccgacgccc tgtgcaggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     5881 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     5941 aggaatcggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     6001 atcgcagccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     6061 gaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc
     6121 agctttaatc accaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     6181 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcactggtgg tcggaaatgg
     6241 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccataagc
     6301 cgagcctcgt tgcagtgtga cataatctgt aatggtttcc gggtaatcgt tatggcaaaa
     6361 gatttgcgtc gaaagatcca cgaccaggtt ttgccccaca ttcacggcgg gcgcaaggtt
     6421 tacataaaca ttggcgctgc caccgccaat agggatagcg gtgccattgg cggttttaca
     6481 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca ggttaataac
     6541 tcgtttcatt acaatcatct cttcggcttc agctgtaggt ataggtgatg ctaatcactg
     6601 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     6661 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca ctattcaatg
     6721 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     6781 aatatccggt actgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     6841 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacatca
     6901 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtgcacg
     6961 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     7021 ttgcgagcgc caatattgcc gccaatacat acccacgttt gcaccatttc atgagcctct
     7081 ccagttactg atattcaaga gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag
     7141 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     7201 gggtcgtcca cgaaatcgcg gacgatgggg cattaatggg tatctgattt tgttgctcat
     7261 tcagaagctg tattcccagt cccgcaaccg ctgacaccgt attttcaagt gcaagcaggt
     7321 tggcattgtg gctatctgca acaccggtaa acccaacttt tacggcagaa acggcattac
     7381 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     7441 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     7501 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaagcgt
     7561 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     7621 ccccttaacg acattcagct gatagctgag ttaataactg ctgctgactc tctggtggca
     7681 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     7741 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     7801 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgggttagcg
     7861 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacggg
     7921 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccagg gtattggtat
     7981 ccagcgctac tctgttctcc cgatattcag tggcataagg cagcacggca taaccgcgcc
     8041 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     8101 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccagta
     8161 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatgt
     8221 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     8281 catcacctcc cccggcgtaa ccggtttgca cgctatagct gaggttgttg tcttccagca
     8341 acgtaccgta tacaccgacc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     8401 aactggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     8461 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     8521 ggctatagct gagcgtccag ttgatatctt cgaatgcagt atttaaacca gcctggaatt
     8581 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
     8641 atgtgcgccc gagttgctga gtaacggtga gttgtaattt cccgcgtttg ttataagcga
     8701 gattgtaata gtcggtgaat ttcggcttaa cctgaataac accatcctgt gtttcgatgt
     8761 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
     8821 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
     8881 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
     8941 tatccacaga cagagcgccc agtgccccca tgtttttccc gataccgaaa ttaaacgcac
     9001 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
     9061 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
     9121 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
     9181 gtacggtaaa gatctgcgtg ctgccgtcag cctctttaat cgttacctgc aagtcaccac
     9241 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggcggcacc gtactattat
     9301 aaatgtcata cccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
     9361 tcaccggagc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
     9421 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccagcgtca
     9481 gccgggaacg taacggaatt atgtctcgct caagccaggt attgatatgc tgccatttat
     9541 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
     9601 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
     9661 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
     9721 caggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
     9781 ggatcgtcag gttgagtcgc tgctgaccaa catccagatg cgcagtagcg tcatggatca
     9841 ttgaggttaa tggcacgcag gcatcatccg ccagcagatt cataccggag acagaagccg
     9901 tattcagctc catacttgca agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
     9961 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    10021 cgacacgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    10081 cagcctgggg atcatccgct aaaaagcgcg gattaaaata gagttcggca gatgacaaag
    10141 gtgcctgtgc ggcaaaagca caggcgacaa agagccggac aaaaaaacca gccaaacgat
    10201 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    10261 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaataattcc
    10321 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    10381 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    10441 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    10501 aatagggtgt cgggttaatc agagtcagag aattcgcgct acgacgaaat cttaattttt
    10561 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    10621 tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt gacggaatcg
    10681 ctttaacgtt catccagaat aaactttccc gatcctgtgg caattggtta tttgttgcat
    10741 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagaggt ggcgtcacga
    10801 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtgc
    10861 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    10921 cgcgagtcgc acctaaggcc actcccgctt cggcgcgtcc ggcaaccatc attgccatga
    10981 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    11041 ttttattact cactgtcctg ttcctgttat cacattatct gctgaacaat tactgatagg
    11101 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    11161 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    11221 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctacg ccaatattgg
    11281 tggctatccc tggcccctct cccacggaaa gcacatctgg atttttacca tccgcgacac
    11341 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    11401 caaaaggcac cggtgcgcta tcttccccta ccgcatgaaa ccggttactg ctgatttgcc
    11461 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    11521 cgccctgaaa ttgcatattt ccgccgggca gcgtggtgtt ccatttattt ccggccagag
    11581 caaacattgg cagcaacgac gctaatagaa ataatctctt ccttttcatc gttattttta
    11641 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtccct gaacctgggt aggttattga
    11701 tactgaacct tgaaggtcgc atccgcatta gcagcacccg gggttgcctc gccgattgca
    11761 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    11821 aatgtcgcac cgtccagcgt cagctcattt cctgttctgt ccagtatctg cacaccaaca
    11881 tttgttgcgc tacccgcagc tgaactctgc agagccagta ctttaggatg agcactgtca
    11941 atcgcagtcc ccaagaaggc aacagcggct tttgtggcaa cactggtatc gcaatcattc
    12001 agctgaatgt taaaaccgac agcagagctg gttgctccag tctgcttcag actagcggta
    12061 cgaacctggc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    12121 ttaacaactt cccctttaaa gtgaacggtc ccaccattta ccgtcgtggg tgtagtttca
    12181 gccagagccg ctgtagaact cagggacaga gccgacagaa caacaattgc cagagtttta
    12241 attttcatac tgctttcctt tcaaaaaact atttctaaat cgacatgggc agtcgttccg
    12301 tacactttgt tttgtcaacg agtttgactg ccaacactgc acagtttccc cccaaaagat
    12361 gaaacatttg gggccatttt gactcataga ggaaa
//
LOCUS       DAFAVF010000125        12095 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFAVF010000125 DAFAVF010000000
VERSION     DAFAVF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.125"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..603
                     /locus_tag="LGK42_003372"
     CDS             <1..603
                     /locus_tag="LGK42_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659591.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            631..1092
                     /locus_tag="LGK42_003373"
     CDS             631..1092
                     /locus_tag="LGK42_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBK2659592.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1112..5068
                     /locus_tag="LGK42_003374"
                     /pseudo
     CDS             1112..5068
                     /locus_tag="LGK42_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            5066..6754
                     /gene="nrfB"
                     /locus_tag="LGK42_003375"
                     /pseudo
     CDS             5066..6754
                     /gene="nrfB"
                     /locus_tag="LGK42_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6741..9713
                     /gene="nfrA"
                     /locus_tag="LGK42_003376"
     CDS             6741..9713
                     /gene="nfrA"
                     /locus_tag="LGK42_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBK2659593.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATSNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            9714..10604
                     /locus_tag="LGK42_003377"
     CDS             9714..10604
                     /locus_tag="LGK42_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBK2659594.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            10787..11548
                     /gene="envY"
                     /locus_tag="LGK42_003378"
     CDS             10787..11548
                     /gene="envY"
                     /locus_tag="LGK42_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBK2659595.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
BASE COUNT         2992 a         3233 c         3374 g         2496 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg atcattatgg ggcgcaccta ccaccaggaa
       61 aaccgcaccc ccggcagcct gccgggaaca aagacgcaga tgaccatccg ctccaaaacg
      121 tacatgggca gcggatttaa tgagctgaag tttgatgatg cgacagggag agaacaggtc
      181 tacatccacg cgcagaagaa catggatacc gaagtgctca acgaccgtac caccaccgta
      241 aaacacgatc accgcgaaac cgtaaaaaat gaccagacgg tcacgatcca ggaaggtaac
      301 cgccttctta cggtggaaaa aggccacaag atcaccggag tactgaaagg gtctttatct
      361 gaggatgtct ttcaggacag aggcacgatt gccggttcgg tgcatgttga cgctgtaaac
      421 aatggtggcg aaggcgacgg tatacaggct tatacggcga ttaaggaaat tttgctggcc
      481 gtggaggaaa gcaaaattgc gctgacgccg gatggcattc agctacaggt cggggaatcg
      541 acggtaatca ggctgtcgaa ggatggcatc accatcgtgg gcggttctgt tttcatcaac
      601 tgattcactc agattatcag ggagtatttt atgcagttca cttttaatga aggccacatt
      661 caactgccgt ctcaatggca ggatcagtca atgcaggtcc tggtgtccac agataacagt
      721 ggcatcaacc tggtcattac ccgcgaagcc gttccacagg gaaccctgac accagagctg
      781 taccaggaaa cgctggcgct gtaccagggc aaacttgatg ggtacaccga acatgcctgc
      841 cgggaaatca ctctggcaga ggccccggcc tggctgctgg attattcctg gaacggtccg
      901 gaagatgagg ggaatcaggg gcggattagc cagatagccg tattccagcg gcgtggagat
      961 acgctgctga ccttcacttt cagcacctcc ttatccctga agaacagtca aaagacgatg
     1021 ctgctggagg tgataaagag cttcaccccg ttgccaccgg agaatgacat ccaaaaggac
     1081 cagccgcgct gacataaacg agggataaca catgagtgaa ggaccaggcg ggccacaggg
     1141 agcgaccgca ggcggtacgc tggcaatgcg aatgctgtca cagcaggcga tggtcgccag
     1201 ccagatgaaa cgggcagcca acgacaaagc cattgcacag atgctggcat caaaaaagtc
     1261 tggccccccc gccgccaggc tgggcgatga aattcagcac aagagttttt tgggggcgct
     1321 ggcaggggcc gtgctggggg cgatagtgac catcgcagaa ggttgcctga ttatggccgc
     1381 ctgtgccacc ggcccttatg cgctggttct ggtgcctgcg ctgatgtatg ccagctataa
     1441 ggcgagtgat tatgtggagg agaaacagaa ccagcttgag tcatggataa acagtttttg
     1501 tgacacggac ggcgccatca ataccggttc tgaaaacgta aaaattaacg gaaagcccgc
     1561 tgccagggcc gccgtcaccc ttccccctcc tcccccacct ggagcaatac ctgaagtccc
     1621 acagggggaa ccctcatggg gtgatattgc cactgacctg cttgaatcgg cagcggaaaa
     1681 agcagtacca ctggcgaagg cctgggggaa cgctgttatc accctgacgg acagcaatgc
     1741 cggttttatg gatcgcgtca gcgccggcgc atcgcttctg tttcccgccg gtccggtatt
     1801 aatggagttt gccaccatgg tgggcgggcg tggcgaaatc aaaaaagatg tggatttccc
     1861 ggaagccggt gaggacacgg cgctctgcga caaggagaac aaaccaccga ggatagccca
     1921 gggcagcagc aacgtcttta tcaacaatca gcctgccgca cgccagggcg acaaactgga
     1981 gtgcagcgca gcaatcgtgg gaggttcgcc ggacgtcttt attggcggtg agcaggtgac
     2041 ctatctggat atccagccgg agttcccgcc gtggcagaga atgatcctgg ggggaataac
     2101 gatagccagc tatcttctgc cgccagcggg actgctggga aaactgaaga atctggcgag
     2161 actcggcaaa ctggggaacc tgctggggaa aagcgggaag ctgctggggg caaagctcgg
     2221 cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag tcatcagatg
     2281 ggtaacagat cctgtcgatc cggtaaccgg cgcgtactgc gacgaacgta ccgacttcac
     2341 cctgggccag acactccccc tctccttcac ccgtttccac agttctgtac tgccgctgca
     2401 tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg tgcgtgaaca
     2461 gggaaaccgg gtggatatca tcagcctggg agccacgctg aacttcgcct tcgacggtga
     2521 aagtgatacg gcggttaacc cgtatcacgc ccagtacatc ctgcgccgcc gtgatgatta
     2581 cctggagctg ttcgaccgtg atgccctgag cagccgcttc ttttatgacg cctttccggg
     2641 aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg cacacagccc
     2701 cggtgaccgg atgtatatgc tgggcgggat gagtgacacc gccagcaacc gcatcacgtt
     2761 tgagcgcgac agccagtacc ggatcacggg tgtcagtcac accgacggga tccggcttaa
     2821 actgacgtac catgccagcg gctacctgaa agccattcac cgcacggata acggcataca
     2881 gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg atgcgcggct
     2941 ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct ggtccgataa
     3001 cgaccagacg tggagccgtt tcacctacga tgcacagggc cggtgcgtga atgtcaccgg
     3061 ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca ccgtgacgga
     3121 cggcaagggc attcaccgtt attactatga tcctgacggc aatattctgc gggaagaagc
     3181 gccggacggc agcaccacca cgtatgaatg ggatgaattc catcacctgc tggcccgcca
     3241 ctcccctgcc gggcgggtgg agaagtttga atacaacgcc gcacacggtc agttaagccg
     3301 ttacacggcg gcagacggcg cggagtggtt gtaccgctat gatgagcgcg gcctgttcag
     3361 caacatcacc gaccctgccg ggcagacgtg gacacagctg tgcgatgaac gcggcctgcc
     3421 ggtaagcctg gtgtcgccac agggcgaaga gacccggctg gcgtacaccg ctcaggggct
     3481 gttgtcggga atattccgcc aggatgaacg gcgtctgggc atagagtacg accaccacaa
     3541 ccggccggaa acactcaccg acgtgatggg ccgtgaacac cacaccgaat acagcggtca
     3601 cgacctgccg gtgaagatgc gcggccccgg cggtcagtca gtgcggttac agtggcagca
     3661 gcaccataaa ctgagcggca ttgaacgtgc tggaaccggc gcggaaggat ttcgctatga
     3721 ccgccacggc aacctgctgg cgtacacgga cggcaatggt gttgtctgga caatggagta
     3781 cggcccgttc gatttgccgg tggcgcgaac ggacggtgaa ggccaccgct ggcagtaccg
     3841 ctacgataaa gacacgctgc aactgacaga agtcattaac ccgcagggtg aatcatatct
     3901 ttatgttctg gataactgcg gccgggtgac ggaagagcat gactggggcg gcgtggtctg
     3961 gcgttaccgc tatgacgccg atggcctctg taccgccagg gttaacggcc tggaggaaac
     4021 catcctctac agccgggatg ccgcaggccg cctggcagaa gtcatctcgc cggaagggaa
     4081 aacgcagtat gcctatgaca aatccggcag gctgacgggt atcttcagcc cggacggcac
     4141 atcacagcgc accggctacg acgaacgcgg ccgggtgaat gtcaccactc agggccgacg
     4201 ggccattgaa taccactacc ccgatgaaca caccgtcatc cgctgtatcc tgccaccgga
     4261 agatgaacgc gacagacatc ccgatgaatc cctgctgaaa accacctacc gttacaacgc
     4321 cgccggagaa ctggcagagg ttatcctgcc gggggatgag acgctgacgt tcagccgtga
     4381 tgaggcggga cgtgaagtgc tccggcacag taaccggggt tttgcctgtg aacagggctg
     4441 gaatgcagcc ggtcagcctg tcagccagcg cgccggattt tttccggaag aagccacatg
     4501 gggcgggctg gttccctcac tggtacggga gtaccgttac gacagcgcgg gtaacgtgtc
     4561 aggcgtcacc agccgggaag attacggacg ggaaacacgg cgggaatacc ggctggaccg
     4621 gaacggccag gtcacggcgg tgacagcctc aggcaccggg ctgggctatg gcgaaggcga
     4681 tgagtcctat ggctatgaca gttgtggcta cctgaaggcg cagtctgcgg gcaggcaccg
     4741 gataagtgaa gagactgacc agtatgccgg aggccaccgg ctgaaacagg ccggaaacac
     4801 gcagtatgac tatgacgccg caggccggat ggtcagccgg acaaaacacc gtgacggcta
     4861 tcgcccggaa acagagcggt tccggtggga cagccgggac cagctgaccg ggtattgcag
     4921 cgcacagggt gagctgtggg aataccgcca cgacgccagc ggcagacgaa cagagaaacg
     4981 ctgcgaccgg aagaaaatcc gcttcacgta tctgtgggac ggcgacagta ttgcggaaat
     5041 ccgggaatac cgcgatgata aactgtacat ggtcgagcgt aaagatctga ttcagatccc
     5101 ggtgtatccg ttcgaacgcg aatggacgca cttcaccagc atgacttaca ttgatgagtt
     5161 ttcagagctg catggcaaag atgttccggt gcgtgaagcc ctcgccggac aagtgcccag
     5221 cgcaggcgtc ggcacctgtt tcagccgccg cgccgtgacc gctctgttag ctgacggtga
     5281 cggtattgct ttcgacgtgc agagtctgac tgaagattac gacattggtt tccgcctgaa
     5341 agagaaaggt atgacggaaa tttttgtccg ttttccggtg gtggacgaag ccaaagaacg
     5401 cgagcagcgc aaatttttac agcacgcacg gacgtcaaac atgatctgcg tgcgcgaata
     5461 tttccccgat accttttcga ctgcagttcg acaaaaatct cgctggatca tcggcattgt
     5521 tttccagggc tttaaaaccc acaaatggac ctccagcctg acgctgaact actttctctg
     5581 gcgcgaccgc aaaggggcaa tcagtaactt tgtcagcttc ctcgcgatgc tggtgatgct
     5641 ccagcttttg ctgttgctgg cgtatgaaag tttgtggccc aatgcctggc atttcctttc
     5701 tatttttagc ggcagcgcat ggttaatgac cctgctgtgg ctaaactttg gcttgatggt
     5761 taaccgcatc gtgcagcggg tgattttcgt cactggctac tacggtctga cgcaggggct
     5821 gctatctgtc ctgcgtcttt tctggggcaa cctgattaac ttcatggcca actggcgcgc
     5881 gctaaaacag gtacttcaac acggcgatcc acgtcgcgtc gcgtgggata aaacaacgca
     5941 tgacttcccc agcgtgactg gcgatacccg ctcgttgcgc ccgttaggcc aaattctgct
     6001 ggaaaatcag gtcatcactg aagaacaact cgatacagca ctgcgtaatc gcgtcgaagg
     6061 tctacgcctg ggcggttcaa tgctgatgca ggggctgatt agcgccgagc agctggcaca
     6121 ggcgctggca gagcaaaacg gcgtggcgtg ggaatccatc gatgcctggc agatcccgtc
     6181 ctcgctgatt gccgaaatgc cagcctccgt agcgctgcat tatgcagtac tgccgctgcg
     6241 tctggaaaat gatgagttaa ttgtcggcag tgaagatggt attgacccgg tttcgctggc
     6301 ggccctgacg cgtaaagtcg gacgcaaagt gcgttacgtc attgttctgc ggggacaaat
     6361 tgtcacgggg ttacgtcact ggtatgcacg ccgacgcggt cacgatccgc gggcaatgtt
     6421 gtacaatgcg gttcagcatc agtggctcac ggaacagcag accggtgaaa tctggcggca
     6481 atatgtgccg catcagttcc tgttcgccga aatactgacc acgctcggtc atattaatcg
     6541 ttcagcaatt aacgtgttgt tattgcgcca tgaacgcagt tctctgccgc tcggcaagtt
     6601 tttggtcacc gaaggcgtta tcagccagga aacgttggat cgcgtcctga caattcaacg
     6661 cgaattacaa gtttcgatgc aatcactatt actcaaagca ggtttaaaca cagaacaggt
     6721 tgcgcaactg gagtccgaaa atgaaggaga ataaccttaa tcgcgtcatc ggatggtctg
     6781 gtttactgct gacgtcttta ttgagtacca gcgcactcgc agacaatatc ggcaccagcg
     6841 cagaagagct ggggctgagc gattatcgcc attttgttat ttatccccgt ctcgataagg
     6901 cgctgaaggc acagaaaaat aacgacgaag caaccgccat ccgcgaattt gaatatatac
     6961 accagcaggt gccggataat attccgctga ctttatacct tgcggaagcc tatcgccatt
     7021 ttggtcatga tgaccgggcg cggctgttgc ttgaggatca actgaaacgt cacccaggag
     7081 atgcccgact tgagcgcagt ctggcggcta ttccggttga agtgaaaagc gttacgactg
     7141 ttgaagaact gcttgcccag caaaaagcgt gcgacgctgc gccgaccctg cgttgtcgca
     7201 gtgaagtcgg gcagaatgcc ctgcggctgg cacaattacc tgtcgccaga gcgcaactga
     7261 acgatgcgac gtttgctgca tcgccagaag gaaaaacgct gcgaaccgat ctgctgcaac
     7321 gggcaatcta cctgaaacaa tggtcccagg cagatacgct atacaatgaa gcacgccagc
     7381 agaacacatt aagcgcggca gaacgccgtc agtggtttga cgtgcttctt gccgggcagc
     7441 tggacgatcg gatcctggca ctgcaatcac aggggatctt caccgatcct cagtcatata
     7501 ttacttacgc gaccgcgctg gcttatcgtg gcgaaaaagc acgcctccag cattatctca
     7561 atgaaaataa gccgctgttt accacggacg cacaagagaa aagttggctc tatctgttat
     7621 ctaaatacag cgccaacccc gttcaggcat tggcgaatta tacggtgcag tttgccgata
     7681 accgccagta tgttgttggc gcgacgctac cggtgctgtt aaaagaaggt cagtacgacg
     7741 cagcgcaaaa actgctcgcc accctccccg ccaatgaaat gcttgaggag cgttatgctg
     7801 tcagcgtggc gaccagtaac aaggctgaat ctctgcgtct ggcacgattg ctgtatcagc
     7861 aagaaccggc aaatcttacc cgcctggatc aactgacctg gcaactgatg cagaacgagc
     7921 agtcacgcga agctgccgat ttattgctgc aacgctatcc tttccagggc gatgcgcgtg
     7981 tcagccagac tttaatggcg cgactggcgt ctctgctgga aagtcatcct tacctggcaa
     8041 cgccggcgaa ggtggcgatt ttatcgaaac ccttaccgct ggcggagcaa cgtcagtggc
     8101 aaagtcagtt gccgggtatt gcagataatt gcccggcaat agttcgcttg ctgggcgata
     8161 tgtcgccttc ctacgatgct gccgcctgga accgtctggc aaagtgttat cgggacacgc
     8221 tacccggtgt ggcgctgtat gcatggcttc aggccgaaca acgacaaccg aacgcctggc
     8281 aacatcgtgc ggtagcctat caggcgtatc aggttgagga ctacgccacc gcactggcgg
     8341 cctggcagaa aatcagtctt cacgacatga gcaatgagga tctgcttgct gctgccaata
     8401 ccgcccaggc ggcaggaaat ggtgcagctc gcgatcgctg gctacaacag gcagaacaac
     8461 gtggactggg aaacaatgcc ctctactggt ggctgcatgc gcaacgttac attcctggtc
     8521 agccggaact cgcactgagc gatctcacgc gctcaatcaa tattgcgcct tctgccaacg
     8581 cttacgttgc gcgggcgaca atttatcgcc aacgtcataa tgtcccggcg gcggtgagtg
     8641 atttgcgcgc cgcgctggaa ctggaaccga ataatagcaa catccaggca gcgcttggtt
     8701 acgccttgtg ggatagcggt gatatcgcac agtcgcggga aatgctcgaa caggcgcata
     8761 aagggcttcc ggacgatccg gcactgatcc gacaactggc ctacgtgaac cagcgtctgg
     8821 atgacatgcc tgcgacgcag cactacgccc ggctggtgat tgatgacatt gataatcagg
     8881 cgctgataac cccactgacc ccagagcaaa atcagcaacg cttcaatttc cgccgtttgc
     8941 atgaggaggt cggtcgccgc tggacgttca gtttcgattc ttctatcggc ttgcgttccg
     9001 gggcaatgag caccgctaac aataatgtcg gcggcgcagc gccagggaaa agctatcgta
     9061 gctacgggca actggaagcc gagtaccgca tcggacgcaa tatgctgctg gaaggcgacc
     9121 tgctctcagt ttatagccgc gtctttgccg ataccggaga aaacggggtg atgatgccgg
     9181 tgaaaaatcc gatgtccggc accggtctgc gctggaagcc gctgcgcgat cagatctttt
     9241 tcctcgccgt cgaacagcag ttgccgctga acgggcaaaa tggcgcatcc gataccatgc
     9301 tgcgcgccag cgcctcattc tttaatggcg gcaaatacag cgacgaatgg cacccgaacg
     9361 gttcaggctg gtttgcccaa aacctgtacc tcgatgcggc gcaatatatc cgccaggata
     9421 ttcaggcgtg gacggcagat tatcgcgtca gctggcatca gaaggtagct aacggacaga
     9481 ctattgagcc ttacgctcac gttcaggaca acggctatcg tgataaaggc actcagggcg
     9541 cgcagcttgg cggagtcggg gtccgctgga atatctggac cggcgagacg cactacgacg
     9601 cctggccgca caaagtcagt ctcggcgtcg agtatcaaca tacctttaag gcgattaatc
     9661 aacgtaacgg agagcgcaac aacgcgtttc tcaccattgg agtgcactgg taaatgcgta
     9721 agttcatttt cgtattgctg acactgcttt tggtcagccc tttttccttt gcgatgaaag
     9781 gtattatctg gcaaccacaa aaccgagata gtcaggttac cgatacccag tggcaggggc
     9841 tgatgagtca gttacgtttg caaggcttcg ataccctggt tttgcaatgg acccgttacg
     9901 gcgatgcatt cacccagcca gaacagcgcg cgttattgtt taagcgtgcc gcagctgcgc
     9961 aacaggctgg cctgaagctg attgtcgggc tgaacgccga tccggaattt tttatgcacc
    10021 agaaacagtc gtccgcagcg ctggaaagct atcttaatcg cctgctggct gccgatctcc
    10081 agcaagccag attatggagc gccgcgcctg gcgtaacgcc ggatggctgg tacatcagcg
    10141 cggaaattga cgacctgaac tggcgcagcg aagctgcccg tcagcctttg ctaacatggt
    10201 taaacaacgc gcagcggctg attagcgatg tttcagcaaa accggtttat atcagtagtt
    10261 ttttcgccgg aaacatgtcg cccgatggct atcgccaact gctggaacac gttaaagcaa
    10321 ccggcgttaa tgtttgggta caggatggca gcggcgtgga taaactgacc gctgaacagc
    10381 gtgaacgtta tctacaggcc agcgccgatt gccaaagttc cgccccagca agcggcattg
    10441 tttatgaact gtttgtcgcc ggcaaaggca aaacctttac agcgaaaccg aaaccggacg
    10501 cagagatcgc ctcgctgtta gcgaaacgtt cctcttgcgg taaagacact ctctatttct
    10561 ctctgcgcta tttgcccgtc gcgcacggca ttctcgagta ttaaatctcc tccaggtaag
    10621 tcgggtacga cctggcttac ctctttcgct ctcgtcagaa ttccatcaag attaattgct
    10681 aaaaagcgtg ctaaagaaag aagatcgtta tcggcgaacg ggccgatcac tcatgcagga
    10741 agcatagatc ctttgagcgg agtttctgat atctgaggag tgcgaaatgc aattgagcag
    10801 cagtgaacct tgcgtggtga tcctgaccga aaaagaggta gaggtaagcg tcaataacca
    10861 tgctacgttt acccttccga aaaactacct ggccgccttc gcgtgcaaca ataacgtcat
    10921 tgaactctca acgttaaatc acgtattaat cacccacatc aaccgtaaca tcatcaacga
    10981 ttatctgttg tttttaaata agaacttaac ctgtgtaaag ccctggtcgc ggctggcaac
    11041 cccggttatt gcttgtcata gccgtacacc ggaagtgttc cggctagccg ccaaccacag
    11101 caagcagcaa cccagcaaac cctgcgaagc ggagttgacg cgcgtattgc tttttaccgt
    11161 attgtctaac tttcttgagc aatcgcggtt tattgcccta ctgatgtata tcttacgcag
    11221 cagcgtccgc gacagcgtct gccgcattat tcaaagcgat attcagcatt actggaatct
    11281 gcgaattgtc gccagttcgc tatgtttaag ccccagcctg ctcaaaaaga aattaaaaaa
    11341 cgaaaatacc agctatagcc agattgtcac agagtgtcgt atgcgttacg ccgtacagat
    11401 gttattgatg gataacaaaa atatcactca ggtggcgcaa ttatgtggct atagcagcac
    11461 gtcatacttt atctctgttt ttaaggcgtt ttacggcctg acaccgttga attatctcgc
    11521 caaacagcga caaaaagtga tgtggtgaag ggcaaagcgg aaacggataa gacgggcata
    11581 aatgaagaag aaatggcgcg ccctgcagga ttcgaacctg cggcccacga cttagaagtt
    11641 cctagaacga cattttaagt caacaaatta ccgcgccatc tctgcgctca cacgtcccac
    11701 tacctcaaaa catgtaaagc cttgcaagcc attgtgaggc cttatgtgtc tcagttttgt
    11761 ccctcttttt tgtactaaaa aacatagcaa ttgaggataa acctcatgct attttcgctt
    11821 atatgccttt aaaggcatgg cgcttaaata gataaaaaca ccacaaaagc ataaaaaacc
    11881 acacagtaaa accgcaacat aaaacaataa cagataatta aaccaaaaac agatagcgca
    11941 ttgtgataat cattcaatac taaacaaaat ataaacagtg gagcaatatg tgattgactc
    12001 attaagttag atataaaaaa tacatattca atcattaaaa cgattgaatg gagaactttt
    12061 atgcgggcga aacttctggg aatagtcctg acaac
//
LOCUS       DAFAVF010000126        12019 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFAVF010000126 DAFAVF010000000
VERSION     DAFAVF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.126"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            149..2326
                     /gene="iroN"
                     /locus_tag="LGK42_003379"
     CDS             149..2326
                     /gene="iroN"
                     /locus_tag="LGK42_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HBK2659596.1"
                     /translation="MRTHKLLWPLTALLVGFNSQVSVAKSSDDDNDETLVVEATAEQI
                     LKQQPGVSVITSEDIKKSPPVNDISDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHASLSLYTNQPESSDEGATRRANFSLSGPMAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDRDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2371..3327)
                     /gene="iroE"
                     /locus_tag="LGK42_003380"
     CDS             complement(2371..3327)
                     /gene="iroE"
                     /locus_tag="LGK42_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HBK2659597.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3412..4641)
                     /gene="iroD"
                     /locus_tag="LGK42_003381"
     CDS             complement(3412..4641)
                     /gene="iroD"
                     /locus_tag="LGK42_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HBK2659598.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKVPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4745..8404)
                     /gene="iroC"
                     /locus_tag="LGK42_003382"
     CDS             complement(4745..8404)
                     /gene="iroC"
                     /locus_tag="LGK42_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HBK2659599.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSTIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGMRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTETSYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLSAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEESPG"
     gene            complement(8544..9659)
                     /gene="iroB"
                     /locus_tag="LGK42_003383"
     CDS             complement(8544..9659)
                     /gene="iroB"
                     /locus_tag="LGK42_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HBK2659600.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            complement(10895..11993)
                     /locus_tag="LGK42_003384"
                     /pseudo
     CDS             complement(10895..11993)
                     /locus_tag="LGK42_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2861 a         3202 c         3168 g         2788 t
ORIGIN      
        1 catgtctctt aggattgcct tgattcctgt taaatggtat ttattatcat tctcgtaata
       61 aaaatcataa ttgcagttaa acgtatctgg aaaggatgtg agcttaacga tcaaacattt
      121 taagttaggc atttattagg gaataggaat gagaactcac aagctcctct ggccgctaac
      181 cgcgctcctg gttgggttta atagccaggt atcagtagct aaatcctccg acgacgataa
      241 tgacgagact ctggtggtgg aagccaccgc tgagcagata ttaaaacagc agccgggagt
      301 gtcggttatt accagcgagg atattaaaaa gtcccctccg gtaaacgaca tttcagatat
      361 tattcgtaaa atgcctggtg tcaatcttac cggcaatagc gcctcgggca cacgcggtaa
      421 taaccgccaa atcgatattc gcggcatggg gccggaaaac accttaattt taattgatgg
      481 tgtaccggtg acgtcacgta actccgtgcg ttatagctgg cgtggagagc gtgatacccg
      541 cggtgacacc aactgggtgc caccggaaca ggttgagcgt attgaagtga tccgcggccc
      601 tgcggcggcg cgctacggtt cgggggcagc cgggggggtg gtgaacatca ttaccaaacg
      661 tcccaccaac gactggcacg cttcgctgtc gttatacacc aaccagccgg aaagtagcga
      721 tgagggcgct acgcgtcgcg ccaatttcag ccttagtggg cctatggctg gtaatgctct
      781 taccacgcgt ttgtatggta acctgaataa aacggatgct gacagttggg atattaattc
      841 tccggtcggt acgaaaaacg cagccgggca tgaaggggta cgtaacaaag atattaacgg
      901 cgttgtctcg tggaaattaa atccgcagca gattctcgat ttcgaagccg gatatagccg
      961 ccaggggaat atctatgcgg gcgatacgca aaacagttct tccagtgcag ttaccgaaag
     1021 cctggcaaaa tccggcaaag agacgaaccg cctgtaccga cagaattatg gtattacgca
     1081 taatggtatc tgggactggg gacaaagtcg ctttggtgtt tattacgaga aaaccaataa
     1141 tacccgcatg aatgaaggat tatccggcgg tggtgaaggg cgtattttag cgggtgaaaa
     1201 gtttacgacc aatcgcctga gttcctggcg aaccagcggc gagcttaata ttcctttgaa
     1261 tgtgatggtt gatcaaacgc tgaccgttgg tgcagagtgg aaccgcgata agctcgatga
     1321 tccttcctct accagcctga cggtgaatga cagagatatc agcggtattt ctggctctgc
     1381 tgcggatcgc agcagtaaaa atcattctca aatcagtgcg ctgtatattg aagataacat
     1441 tgagccggtt cctggcacga atatcattcc cggcctgcgc tttgattatc tcagcgactc
     1501 cggcgggaac ttcagcccca gtctgaatct ttcgcaggaa ttgggcgatt atttcaaagt
     1561 caaagcaggg gttgcccgaa cctttaaagc cccaaacctg tatcaatcca gtgaaggcta
     1621 tctgctctac tcgaaaggca atggctgtcc aaaagatatt acatcaggcg ggtgctacct
     1681 gatcggtaat aaagatctcg atccggaaat cagcgtcaat aaagaaattg ggctggagtt
     1741 cacctgggaa gattaccacg caagtgtgac ctacttccgc aatgattacc agaataagat
     1801 cgtggccggg gataacgtta tcgggcaaac cgcttcaggc gcatatatcc tcaagtggca
     1861 gaatggcggg aaagctctgg tggacggtat cgaagccagt atgtctttcc cactggtgaa
     1921 agatcgtctg aactggaata ccaatgccac atggatgatc acttcggagc aaaaagacac
     1981 cggtaatcct ctgtcggtca tcccgaaata tactatcaat aactcgctta actggaccat
     2041 cacccaggcg ttttctgcca gcgtcaactg gacgttatat ggcagacaaa aaccgcgtac
     2101 tcatgcggaa acccgcagtg aagatactgg cggtctgtca ggtaaagagc tgggcgctta
     2161 ttcactggtg gggacgaact tcaattacga tattaataaa aatctacgtc ttaatgtcgg
     2221 cgtcagtaat atcctcaata aacagatctt ccgatcttct gaaggggcga atacctataa
     2281 cgagccaggc cgggcttact atgccggagt taccgcatca ttctgatcgt agtgtttcag
     2341 gcgtactgcc cgcgtaaaag cgggcagtgt ttagtggctt aactcatgac aacctgctgt
     2401 gtaatttgcg ttttcaccac tgatatctaa cagtgcctgg cgaaaggagg cattgaacat
     2461 cggcccgtgt cccaggttgg ggaaatccca aaatacggca ttgacgcctt tatctttcag
     2521 tatggtgagg gtggtatgaa ttttcgacag caccccgaca gcatgcgttt cccggttatc
     2581 accctgtgtc gccgagcctt ccattatcgc caggtgtttg gcgcagaatt gcagaggctc
     2641 aaccgccgta acgcggctta gcaaagcatc ataacctctg cccaacgacg ggctggcgct
     2701 gtagtacgac cggaaataag aggaggacag ccaggaatcc agcacgaaga ggccgccgta
     2761 ggagtgcccc cataagccgc ggcgttgccg atcgatattc agtccctgtt ccacttttgg
     2821 ggcaatacgc gtttccagta actggcggaa gttgttgctg ccaccactct tacggctaaa
     2881 acgccctgag tggagatctg tttttctact ttctgctgct ggcgtatagt cgtaagccct
     2941 gctgttgaga tcgaaaggga ggttggtctg atacccgaca gccacgatca ctggcggtgt
     3001 tttttctgac aattgtttga gcagttcgtc atccaggcga tccataactg cgttaccgtc
     3061 aagcatatat aaaatcgggt aacccgatgc cggtgcggtt gtattcggca cggccgtcca
     3121 tacccgataa tggcgtgtgc catcgacaga gtcgaaagag gtgacgctga aatggtaaaa
     3181 cacggagcct ttatcggcta tattcggccc cagtggccgc atatccggct tcgcataaac
     3241 ttgcgcacta cagataaggg tgaggcaaga aagatgaaag aaaatcgctt tatgcgggcg
     3301 tgttgagcga tactcgcggg catacatctg atgattattc cttttccgca ggtgactatt
     3361 tgatttattc aggaagtctg taccgtttcc tgaaagtgtt tgtgggtcaa ctcaaccctg
     3421 tagtaaacca atcccgtcaa tcagtgcacc gcgccaccat gcgtaatcgt gaccaccggt
     3481 gtagattgcg caatggcttt cgacgccagc ggtaatcagc cgctggtgaa gctgctgaac
     3541 gtgaggcact gtcgaacctt ccagcgatcc cacgcacagg ctgatacgta cgccctgcgg
     3601 tggggcagaa agcagatgct cactcaccca tgaggtatcg gtttcgctga acaagcctgg
     3661 tcgactggtt ctttctggcg tccaccacat tgaaggagag tggctgagca ccagaccgaa
     3721 cgtttccggt gcgtaacgag cccccattag cgcactgatc ccgccgaggc tctgcccggc
     3781 cagcactgtg cgcgaacggt ctgcccactg acgctgcggt tgttcagcgc gaatcatcgg
     3841 cagcagatgt ccggcgatat cctttatcag tttgctgcgc ccgccgagta tctcagtgcg
     3901 ttcatgttca ttaatgttgt caatgcccag tacagccacg ggcacgatgc gtccgttatt
     3961 tatggcggcg tcaattgctg cgcatacgcc aaggtgatca aaccaggttt caccatcggg
     4021 cagcacgacc agcccgaggg gctgagaaat atcaacgtcc ggaatataga gccgaacctg
     4081 gcgagatttc ccggcgataa tgcgcatgga ggtaagcagt tgtccggtgt ggaagccgcc
     4141 gttccattcc gacagggcgg gagctttatc aagtgtcagc actgattcct ttgcgtttcc
     4201 ccgaacgttg atctccggca ctttgtttag cggatcggcc tttccggcaa gggtggcaaa
     4261 acggcctccg gacagtgcaa tcgtctcagc cgtagtgccg ggggggattt ccagcagcga
     4321 ataggagccg cagtaacttg cgggtaaacg cagtgtcagt gtccagatat ccgtttcggg
     4381 aagggcgctc atcattcctt tttctacgtg ctctttatcc gtcacccggt tcagacgcag
     4441 ataaacgccc tgcagaggat gtcgggcgcg ccacagaaag gtcacttctc gctcatcttc
     4501 cgctccttcg actggcgtca caagaggaac attcagcgac tcagcttcgc gccagaagtc
     4561 agtaaggtta gcaatgtggc ccgctgccag ttggttgcgc aggctggcga tagcagaggg
     4621 atgttgttgc atgttcagca ttcatactct ttaaaaatta aggatgtcag tataaataat
     4681 taaagcggct attcacggta ccgccgcttc tgactgtgtt tttaatacat tacgtgtcca
     4741 gtaactatcc cgggctttcc tccttcatgt tgtgctggcg atggagcgca gtactgctga
     4801 cgctgtcatg ccataagcgg gtatagaggc cgcccgcaga taacagctgt tcgtgggttc
     4861 cgtattccgc aagtaacccc ttatcaataa cggcaatcag atcgcagcgt tgcgcggtgg
     4921 tcagacgatg tgcaacaatc agcgccgagt gcttcccggc atgcacgaca tctgttaacg
     4981 atgacatcag tcgttcttcg gatgtgcgat ccagacatga cgtggcttcg tcgagcagca
     5041 ggatgtgagc attcgccagt tgcgcgcggg ccagcgcaat caactggcgc tgacctgcgg
     5101 acagatcggc tccgccgtta ttcaccggtg tccggaatcc ctgcggcaga ttgcacacca
     5161 tctcatacag ccctgcccgc tgtgaggcaa tttcaacgtc ttcattggtg gcggatggcc
     5221 gtgaataacg aatgttttct gcaatatcac tactaaacag tgctacatcc tgatcgacaa
     5281 gcccaatctg gcgacgatac tcagtgagcg aggcatcatc gagcatttgc acaccgattc
     5341 tgatgttgcc gtgcgtgggg aaatacaacc cggcaatcag cttaatcagc gtcgacttac
     5401 ccgcaccgct gcgaccgacg accgcgacaa ccattccctc tgggatcgtc agggtaagtt
     5461 tgttcagagc tggctcgtga ctgtcgggat aactgaacgt gacctcatca agatgcagtg
     5521 caccggtgac agggaggacc gaagaggacc cgaggttctc agtgccttct gtcgccagta
     5581 gttcatcaat atgtttgccg ctggctgtcg cctgctgcca ggcgtcgaca atccctgata
     5641 actgctgcac tggcccatag aattgcccca gcaacaggaa gaaagccgcc agtaccccgg
     5701 cagtcatttc tccagcggcg acctgcgaag ctcccactaa cagaacagcg gcataggagg
     5761 tctcggtgca gaatgtcagg aacggaaaat agactgcaag gtatttttgt gctcgcacac
     5821 gggttgcgcg gaaacgctcc gataacgcgc gcagccgggc gccctccagt tcctgctgac
     5881 catgcgattg cacgacacgc atgccagaga ctttttcctg cagggtgctg ttgactttgc
     5941 ctatttccag ccgtgcctgt gcataagcag gggtactgag gcggcgataa atcatggttg
     6001 ccagtgcggc cactggcact gcgcttaatg ccgtcagcgc caggaacggg tcgagccaga
     6061 acatggtggc ggcgattgcg aacatcgtca ccagactggt ggccgcaccg gcaaggccgt
     6121 tttgcagaaa gcgggcgaga gagtccacat caacggtcat gcgggtcaga cgcgaatcgg
     6181 catgcttttc atgccagggg agtccaagac gcagcatatg gccgaagctg cgcaagcgca
     6241 ccgaatgctg aactgattcc gccgctctgg cggcgaagac cgtctgcaga gaataactgc
     6301 accagcccac aatgaccagg cacagggcga gcagggcaca gaggccgatg gtcgacatat
     6361 cgcctgcggc gacaccggag tcaatgccgt gctgcaacag tatcagcacc cctacgcctg
     6421 ccgcagaatc gagtgcgatc agcagagcgg cgatgacgaa catccacgcc acaggcttca
     6481 gtagtgacgt gactttgcga cctttaccgg ccagcgcctg ctgtactgca ttttccggta
     6541 cgcgggtcat acgggcgaca aaacctttat cccccgtatc cgttacatcg tcttgtgtcg
     6601 ctggttcagc cggccacaat tcgttgtggc tattatggga cgtggccaga aaatcgctgt
     6661 cgccggtcat cagtgcgcgg aacgccggac agcgcgcttc aagttctgcc ggggttccgg
     6721 tatccaccat acggccctta tccagcacca caacccggct ggctagctga agcgttgaac
     6781 gccgtcgggc aatcaccagc agcatatgcc cttcgtcagc atagcgaccc agcgcggtat
     6841 taatctccgc ttccgtaaca gcatcaaccg ctgaggtggt atcatccagg attaacacgt
     6901 ccggtgcagt aatgagcgcc cgcgccagcg ctattcgctg cctctgcccg ccggaaagat
     6961 tcgtaccccg ttcggttaac aggctatcga agcctttcgg cagggcgttg ataaaatcgc
     7021 tggctcctgc agcagctgcc gcacggcgaa tgtcgtccgc tgttgcctga gggtggccat
     7081 aggcgatatt ttccgccacc gtaccggcaa acagaaaagc atcttcaaat acgatgccta
     7141 cccggcgacg aagcgcctcc agtctgatat cgcgaagatt ttgtcggcct tcgctggtgt
     7201 tgagccatat ctttccggag cagggatcat aaaaacgcgc cagcagcatc aacagggtcg
     7261 attttcctga acccgatgcg cccaccaccg ccacggtttc accggcgcgc agggaaaagg
     7321 agatatcgct gaggatatgt ctgtcgccat ggtggtagtc aaagctcata ttctccagtt
     7381 ccagtccgac aacctgactg ttaatcgact ccgtcccgtc ctccatctgt gaacgggtgt
     7441 cgatcagtgc aaacacccgc tccacggacg cctgagtgcg ctgtgcgata accagaaacg
     7501 atgccagtac gcgggttggc ccggtcagca tcgcgaggaa gctggcgaat gcaacgaagg
     7561 taccgagatc gatccgcccg tgcatgaccg accacccgcc gtagcacagt aggacgattt
     7621 gcccgagcac cggtagcgcc aacatggtgg ccccaggcat cgcctgcgag ataaccgcac
     7681 cgatgcgtac acgcacaatc tgccgcgact gcctatcgag ccagtgcgtt tcacgcatct
     7741 ctgccacaca ggattttacc actgagatct gtgccagcac ctcgcgtata tgttcggtga
     7801 gattcgccag ttggtccgag gccagcccgg tttgtgcgaa gaccctacga cgcgcacgaa
     7861 gcgcggtgat cgccaggcat accagtacgc acacgacgat aagcgtcatg gcaggggaca
     7921 tccacagcat cacggcaatg ccggcaatgt aataagtgaa cactgccagc ggcaccgggc
     7981 acatctgcag cagggtatgc acctgctgca gatcgctgtt ggtccgtgaa atcacctgcc
     8041 cggtgcgcag cgcatcctgg cctgcgccgt caagtttctg aatactgtcg aatacccgtc
     8101 ctctgagggt atgctgcacc cagagtgaga gcatcccggc ataaccacgg cgcacatagt
     8161 taccgatgaa atcaaaaaaa gcgatcaaca gtaacccgca ggccagccac ggcaggcggg
     8221 ccggattgcc cgccagtgcg tcattcacag cctgtcttgt cagcagtggc gtcagcgcag
     8281 ccagcaaaat agtcgatact gacgctacca ccacgatgac actaagtttc ttacgttccc
     8341 agcacacgcg cgctaggcga actatccatg actgagccgg tgtgggagtg tgattcgcag
     8401 gcattaagcc tcctccgtct gtaaaatacg atccactggc cggatcgttc cgcaaaaaag
     8461 ccagcacgct ccggtcccgc attgttatgg atgcatgact cctggatggg tgctggcata
     8521 tgattatcat gaaggtgtag tggctaccct ttctgtacca ttgtgatcag gcttttcgcc
     8581 acttcacctg ggcagggttg cgccgccatt tccgctgcta cctcttcaga ggctttgcga
     8641 agtgagcgat tgttgaggaa agcattgatc atgttactcg acaggccgac gtcgccaggg
     8701 ataatcccgc agccgcgttc ggcaacaacg cgggcattta ccgggcgatc ggctccctgg
     8761 ccgaagacga tttgtggaat accagcgtgt agcgctgtca gggtattgcc tgctccgcca
     8821 tgatgaatga aaccatctgc gccgttgagg aagacaccca tgggtatcca gtcaaccagg
     8881 cgaacatttg acggcagcga gcgcaaatcg gaccgcgcat tggctgaaat atgcaggatg
     8941 atctcggcat cgacctcact ggcagaatcc ataacccagg cgattagatc aaggccgtca
     9001 accatcggtt tgacagtgcc gagactcacc agcagacgtt tacgctcggg ccttctctcc
     9061 caccatggtt cccacaccgc gccaccgtta tacgggacgt actgcatagg aataatgggt
     9121 tcaccgtcgt tttccagaat gctcatactc ggcggtgtta cgtcgatcca ggccatatca
     9181 cgtggtgtgg cgcccacatt gtggcgacga taggcgtccg tgagcgaacg cgttacaccc
     9241 ttgatatgcc agggggtatg gccgaagcct acggtttgca tcacgaccgg tatatcatat
     9301 ttggcggcaa tcagcggccc gatgactccg agcggagggt agatgatgag gtcaggccgc
     9361 cagtgtccgg caaactccac cagatggtcg gccatttctt cgctgaagaa tgagaagttg
     9421 cccatttggg ttccgatatt actttttttc cgctgtgcct catggtggcg gtaaccagct
     9481 tcggaatcca gaccgggtgc ggcgtcaaac accaccaacc cggcctctgc cgctttttgt
     9541 gcgaattgtc ctccgctggc gatcagcact tcatgaccgt taacgcgaaa tgcctgcgcc
     9601 agagacagca caggatacaa cagcccatag agaggcgggc ctacaaacaa aatacgcatg
     9661 agaaaatccc tctccgcttg acgatctaaa tcaggcagtc gacgcactca tatgccagca
     9721 gcaggacact acttttcgtt aaacctgctt taccagatgc tgcgccattt gtgcttcacc
     9781 agcgttatgg acagctcagt cagagacttt tccgtgacat acacaggtga atagggaact
     9841 ccgtatcccc acttccatta acgcttacgc gccattgtcc tgttgaacag cccaaatggc
     9901 gtcggacagc tacgccggga tgttctgacg catttgtgca gtgttagacc gaacaacgaa
     9961 tctggaaaga accgtgattt cttagacaca cgatgcatca tcctgatagt ctcgccttca
    10021 ctggtctgta agtaaaaacg ccgttgtaca ggcgatgttt gttccctttc aaccttacca
    10081 gaaacaatac agacacaaat gctaatgata ataattacca atatcattta actattgcaa
    10141 cggtaattaa aacttaataa aaagggaagc tcccagaggg ctatctgggg atatttaaat
    10201 tgaaacctac gctgctacag accgtggaga atatcttgca aacgctgagc atgctgttcc
    10261 aactcaccgg aaggctgata gacgttaagg cattggtgag cggttctgcc gtcgccaaaa
    10321 atgtatagag aactccgacc agcagcgttt catcctctgt ctatagcgca agcgtgtcga
    10381 gaactactgc tgatattacg ctgttggcga ataatccgat ttttgctcct gaccaggtga
    10441 gttttttcgg cttttcgctg ccagtgtcag gctttcacgt catcattttg gatacgttat
    10501 ttcactaaat ggtcattgtt agggaaaaga gaatgtgtga ttaggtcgtt tagcgttgct
    10561 ttccatcaca ctctagccaa ttcacaacta tgggcggttc tcgacgattc caggtcaggt
    10621 tgacgggacg ctagtggttt caaagcttgg tcaacagata acgattgaaa aagtcatgat
    10681 cttactgaag ttgtgcggat agtgagtaat gccgcgagac gaactggccg ggagattgat
    10741 ggaactttaa aaattatctc ttaacgcatt tacgaatgca aaaagtctat tttttggtta
    10801 gcggagaatt tgttaaaaga atccagcata gtgaatcccc ctgcgcggct gtctcttgat
    10861 cagatctcct gataaagaga cttcatcacc agcctcaacc atatcctgaa gtctgaacca
    10921 gccatcccac atatctaccc aaccggggcg gcctgtgcgt ttgctgtgta tgggtccacc
    10981 acatattgca atttttgttc tcttttaaaa tatattcctc gggcgtcatt agtgcgggcg
    11041 ccttgtggag tcttttgctg ttatacagcg ccagatattc cgccagtttc aggttaaaca
    11101 acgccagatc ctcaaagagt aaaatttcat taaattcaat aaattgctct cttaatgccc
    11161 ggttaaaacg ttcacagata gagttcattt ttggcgtgta gggataggtc cagaggtgtt
    11221 tgatggcggc ttcctgcagc gttttgtcaa agcttcccag gaactctttc ccgttatctg
    11281 tgatgatctg gctgataccg acagggaaca gccgggctgc gcggctgaaa aagtgattga
    11341 cgatatcact gttgagcgac ggcacagcca gcgccagtac gtaatcgctg tgttcgtcga
    11401 tcatggtaat gatatagcga cgcatttcac ccattctgag ctcaatggcg tccatcccaa
    11461 tgagctctcc ggtctttacc ggacggtagt gctttggcct gcggggtttg gtggttcgct
    11521 ttttgacaag cagagcctta ccccgcgagc tcagacgtac tggtatcatc cgcattttat
    11581 cgtgtgcggt ggcgatcatt ctgccgatgg tggaaacact cgggcaggtc tgatggcgtt
    11641 atgcgcacca gggcttcagg cgaacaaaaa tctgctcttt accgaggttc ggcagctctg
    11701 tcctgaggcg tcgaatctct ttcagcacat cgggatgcca gtgctttttt cgtcgcacca
    11761 gagctgcctt actgtgcggg attagcccct caggtcctcc cttgttcagt aactgacgcc
    11821 accagtaaag cgtgcggcag gatacgccaa aggcttcaga agctgcagaa ataccgtgtt
    11881 tatcccagaa gtgcagagcc ttcattctca ttgttgctat atcagccatc actgaaagcc
    11941 tcaacgcata atcggctgct taggatagcc aacctcaggt gctgcatttg catagtgcaa
    12001 tctcccctta cttatccgg
//
LOCUS       DAFAVF010000127        11902 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFAVF010000127 DAFAVF010000000
VERSION     DAFAVF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.127"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..96
                     /locus_tag="LGK42_003385"
     CDS             <1..96
                     /locus_tag="LGK42_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBK2659601.1"
                     /translation="GFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            242..1474
                     /gene="mdtM"
                     /locus_tag="LGK42_003386"
     CDS             242..1474
                     /gene="mdtM"
                     /locus_tag="LGK42_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HBK2659602.1"
                     /translation="MAGLKATHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIRLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            1515..2795
                     /locus_tag="LGK42_003387"
     CDS             1515..2795
                     /locus_tag="LGK42_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBK2659603.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNNRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            2911..4062
                     /locus_tag="LGK42_003388"
     CDS             2911..4062
                     /locus_tag="LGK42_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBK2659604.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4072..4839
                     /locus_tag="LGK42_003389"
     CDS             4072..4839
                     /locus_tag="LGK42_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659605.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            4836..5093
                     /locus_tag="LGK42_003390"
     CDS             4836..5093
                     /locus_tag="LGK42_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBK2659606.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5158..6018
                     /locus_tag="LGK42_003391"
     CDS             5158..6018
                     /locus_tag="LGK42_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBK2659607.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            6086..7264
                     /locus_tag="LGK42_003392"
     CDS             6086..7264
                     /locus_tag="LGK42_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2659608.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7277..7831)
                     /gene="kptA"
                     /locus_tag="LGK42_003393"
     CDS             complement(7277..7831)
                     /gene="kptA"
                     /locus_tag="LGK42_003393"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBK2659609.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8081..8764
                     /locus_tag="LGK42_003394"
     CDS             8081..8764
                     /locus_tag="LGK42_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659610.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8761..9222
                     /locus_tag="LGK42_003395"
     CDS             8761..9222
                     /locus_tag="LGK42_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBK2659611.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLQPNVVMVFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNVKYYPHIITVCVINALLSIWVMQLIV"
     gene            9235..10407
                     /gene="iadA"
                     /locus_tag="LGK42_003396"
     CDS             9235..10407
                     /gene="iadA"
                     /locus_tag="LGK42_003396"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK2659612.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10472..11383
                     /gene="hypT"
                     /locus_tag="LGK42_003397"
                     /gene_synonym="qseD"
     CDS             10472..11383
                     /gene="hypT"
                     /locus_tag="LGK42_003397"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBK2659613.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11376..11768)
                     /gene="iraD"
                     /locus_tag="LGK42_003398"
     CDS             complement(11376..11768)
                     /gene="iraD"
                     /locus_tag="LGK42_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBK2659614.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(11765..11848)
                     /gene="idlP"
                     /locus_tag="LGK42_003399"
     CDS             complement(11765..11848)
                     /gene="idlP"
                     /locus_tag="LGK42_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBK2659615.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2660 a         3010 c         3127 g         3105 t
ORIGIN      
        1 ggattccata atgattccac gccccgttat gacaaatctg ccgccgatct tgcctggcaa
       61 aggacactga aatggtttga taaatatctc tcctgatagg tttatctctt acgggattac
      121 gtcttaaaca agcatgaaaa aatagcgtgc gcaaaagtcg ttctttgcct aaaatatcgc
      181 tatatatggc gatatatagc gaataaagtg aacgatgaat aaccattttg gtaaaggctt
      241 aatggcggga ttaaaagcaa cgcatgccgc cacgctgttt ttcccgatgg cgttgatttt
      301 gtatgatttt gccgcgtacc tgtcgacgga cctgattcag ccggggatca ttaatgtggt
      361 ccgtgatttt aatgccgatg tcagtctggc cccggcagcc gtcagtctct atctcgcggg
      421 cggtatggcg ttacagtggc tgctggggcc gctttccgac aggattggcc gcaggccggt
      481 gctgattacc ggggcgctaa tatttaccct tgcctgcgct gcgacaatgt tcacaacgtc
      541 tatgacacag tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt ttattgccac
      601 cgttggttat gtcacggtgc aggaggcgtt cggccagaca aaagggatca ggttgatggc
      661 gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg gcgcagctct
      721 gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg ggtttatctc
      781 atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg ttccatttag
      841 cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggctgt tcctctttgg
      901 cgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg tctcgccggt
      961 gatcctgatc gacgcgggcg gcttaacaac ttcgcagttc gcctggacac aggttccggt
     1021 gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag atccaaccga
     1081 accgcgcttc atctggcgtg ccgtacccat tcaactggtc ggcctcgcgc tgttgattat
     1141 cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca gtttgtatgc
     1201 tttcgggatt ggtttgatct tcccgacctt attccgcttt acgctttttt ccaataactt
     1261 accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga tgtctgtctc
     1321 ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc
     1381 cgtcgtggcg ggcgttatcg tcgttttcac cctggcagga ttgcttaatc gcgtgcgcca
     1441 acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct
     1501 ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg ttggcgctct
     1561 ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg cccaattttt
     1621 gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg gcggactggt
     1681 ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt catacggcga
     1741 ttatcccgcg taataaagac cggattggcg aaaatctcgg ccagttcgtg caggaaaaat
     1801 ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg ttgttgattg
     1861 gcaactggtt tagtcagccg gagaacgccc gccgcgttgg tcagcatctg ttgcagatca
     1921 tgagcggttt tcttgaactg accgatgatg cgcgtattca gcgcctgctt aagcgcgcgg
     1981 tccatcgggc gattgataag gtcgatcttt ccggcaccag tgcgttgatg ctggagagta
     2041 tgaccaaaaa caatcgtcat caggtgctgc tggatacgct gatcgcacag ttgatcgccc
     2101 ttctccagcg cgataaatcg cgcaagttta ttgcccagca aattgttcgc tggctggaga
     2161 gcgagcatcc gctgaaagcc aaaattttgc ctactgaatg gctgggcgaa catagcgcgg
     2221 agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgcgat cgtgcgcatc
     2281 agatccgcca tgcatttgat cgcgccacct ttgccctgat cgacaagttg aaaaacgatc
     2341 cggaaatggc agcgcgagcc gatgccgtaa aaagttatct gaaagaagat gaagctttta
     2401 accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg gatatcaaca
     2461 gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg tcaatggttt ggcgaaacgt
     2521 taattgccga tgatgccttg cgggcatcgt taaatggtca cctggaacaa gccgcgcacc
     2581 gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgataca gtaaaaagct
     2641 gggatgctcg ggatatgtcg cggcaaatcg agttaaatat cggcaaagat ctgcagttta
     2701 tccgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat ttgttgtcgc
     2761 agctcccggc cttgttcccc ctcagcaatt tttagaaatc catgaaaaac taagtggcta
     2821 accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg cctcttttca
     2881 ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct acccgccatt
     2941 ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga tctcaaggag
     3001 cgcggcattc cgctggttgg cacttactgc acctttatgc cgcaagagat cccgatggca
     3061 gccggtgcgg ttgtggtttc gctctgttct acctctgatg aaaccattga agaagcggag
     3121 aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt cggcaaaacc
     3181 gataaatgcc cctacttcta cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc
     3241 aaaaagaaaa tgtatgaata catggcggag tttaagcccg ttcatgtgat gcagttgccg
     3301 aacagcgtta aggacgatgc ctcgcgtgcg ttatggaaag ccgagatgct gcgcttgcaa
     3361 aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctgcg cgatgccatt
     3421 gcgctgaaaa accgcgaacg tcgcgcactg gccaattttt atcatcttgg gcagttaaat
     3481 cctccggcgc ttagcggcag cgacattctg aaagtggtct acggcgcaac cttccggttc
     3541 gataaagagg cgttgatcaa tgaactggat gcaatgaccg cccgcgttcg tcagcagtgg
     3601 gaagaaggcc agcgactgga cccgcgtcca cgcattttaa tcaccggctg cccgattggc
     3661 ggcgcagcag aaaaagtggt gcgggcaatt gaagagaatg gcggctgggt tgtcggttat
     3721 gaaaactgca ctggggcgaa agcgaccgag caatgcgtgg cggaaacggg cgatgtctac
     3781 gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag
     3841 cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt agttgatgtg
     3901 attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt
     3961 cagcagcaca acattcctta tatcgctatt gaaacagact attccacctc agatgtcggg
     4021 cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtgacatat
     4081 tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc agacggcgtg
     4141 attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat tactgaagcc
     4201 tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac cggctatgga
     4261 cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca tgggctgggc
     4321 gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa
     4381 gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg
     4441 gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa
     4501 ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg cacagtgttt
     4561 gccgaatcag aagtgatcag cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc
     4621 ggagtgatta acgcgatggc gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa
     4681 gcgccgattc tgtttactgg tggcgttagt cattgccaga cgtttgcccg gatgctggaa
     4741 tctcatctgg gaatgccggt aaatacccat cctgatgcgc aatttgctgg cgcaattggc
     4801 gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa agagttttta tttttatttc
     4861 actccacagt cggcgtcata caaacccgca aagcgttgca ggcagcgggc atgacttttc
     4921 gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct
     4981 gtccccccgg cgaggaaata caatgggtga tccctgggct gactgagtca atttattgtc
     5041 agcaggatgg tgtctggcgc tgcattgcac attaccgggt ttctccgcgt taaggattca
     5101 ttaaggtcgc tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg
     5161 acaaaaagta tttcgttaag taaacgcatc tccgttattg tgattctttt tgccattgtg
     5221 gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt
     5281 cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct
     5341 tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc
     5401 attgttgaaa tgaccaccaa tggtgatttt atccgcacaa ttcctctgga ttttgttaag
     5461 gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat
     5521 tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata
     5581 aaaatcccgc tgcaagactc cccaaccaat tgcggttttg aagggttggc ttattctcgc
     5641 caggatcaca ctttctggtt ttttaaagag aaaaatccga ttgaggtcta caaagttaac
     5701 gggctgttaa gtagcaatga attacacatc agcaaagacg aggcactaca acggcaattt
     5761 actttagatg atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct tctggtgctg
     5821 tctcatgaat ctcgcgcact ccaggaagta accctggttg gcgaggttat cggtgaaata
     5881 tcactaacca aaggcagtcg aggtttgtcg cataacatca aacaggccga agggattgcg
     5941 atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc
     6001 cctaaatcat cgcattaaca ggctggcata tttcaccttt ttgttattaa cttgttaaca
     6061 acaaaaatga catacaaggt taaacatgcc ttcgtcctcg catcccgtag aacgcttttc
     6121 tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggat taggtcgctt
     6181 cctttatacg cctatgttgc ccgtcatgat ggcggaaggg gcgttttcat ttagccagct
     6241 ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcgtt
     6301 tggcgcattt caccagccgt cgcgcctgcg cccatttctg ttggtttctg ccctggcgag
     6361 cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt tattgattcg
     6421 cttcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc tgattatgca
     6481 gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca ttggcattgc
     6541 actgggcaac gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg
     6601 gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc
     6661 gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
     6721 gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg tcgcgactta
     6781 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
     6841 agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg
     6901 ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct
     6961 cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat
     7021 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa
     7081 tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac
     7141 cagtatgctc ggcaatggaa catcggcgct tgccagcgcc actctctgcg gcgcggcggc
     7201 gttgtttatt gctgcgctta tcagcacagt gcaattattt aagctacaag tggtcacttc
     7261 atgagggaag tttcccttac cactcaagaa actcaaccgc aaccgttgat gtcagccaga
     7321 ccccgttttc cgcttgccag aaagggaggc ctcgtttcgc catttcctgt gctttgacgg
     7381 ttaaaataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
     7441 agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc tcgtcaagga
     7501 aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag
     7561 cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
     7621 taaaacgctt tttatcgctg gtggcaacca cagtatccag caatgcacgg gttaaccgct
     7681 tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca
     7741 gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
     7801 ccgccagctc tttttcgttg tactttgcca tcgcgttttc gtccctggat tatctgtttt
     7861 ttatttacta tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgtaga
     7921 accttttcca cacagcgtaa ttttaccgct atgcaaaaaa cgcatagtca gaaacatact
     7981 tgcattttcc ccatacccac atggctgata aggtttttca gctccaggaa ttttcctggc
     8041 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
     8101 tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
     8161 caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc gcgtttttga
     8221 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
     8281 gtttcgcggc gcgggtggcg cgggggctaa agatggcttt ctctttgcgc tggagctggc
     8341 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gcggattacg
     8401 cgccgcgcag cagttaatga cgccagtgct aaaaccgttg ttgggcattc cagggatctg
     8461 ctcgctggcg ctgatcgcta acctgcaaaa caccgatgcc gccgcaggta tgaccaaaga
     8521 gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
     8581 cagcggtagc gccattatca ccaactactt ctcttccggc gtcgcggtgt ttgcctttct
     8641 gggcacgtcc gtaatcgtac ctttagcagt catcctggtg tttaaatttg tcggagccaa
     8701 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
     8761 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggtgc gcgccgtggc
     8821 tttactatcg ccacgaccaa cctgcagcca aacgtggtga tggtgtttgt catcattcag
     8881 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
     8941 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggt
     9001 ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc
     9061 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
     9121 ctggggacag ccgaagtgaa tgtcaaatat tacccgcata tcattacggt ctgcgtgatt
     9181 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
     9241 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
     9301 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagtaatatc
     9361 ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat cctctgccca
     9421 ggttttattg atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg
     9481 cgcacgccgg aagtggcgtt aagtcgtctg acggaagcag gcgtcacgtc agtggttggt
     9541 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
     9601 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
     9661 actattacgg gttccgtgga aaaagacgtg gcgattatcg atcgcgtgat tggcgtgaaa
     9721 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
     9781 gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg
     9841 ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg cgatgtgccg
     9901 attagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg
     9961 gagttcgcgc gcaaaggcgg caccatcgat atcaccagca gcattgacga accagtcgcc
    10021 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    10081 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    10141 ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agattatgat
    10201 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    10261 agcgggaaag gcgaaattct gccaggcaat gacgcagact tactggtcat gacgccagaa
    10321 ttacgcattg agcaggtata cgctcgcggc aagctgatgg tcaaagacgg caaagcctgc
    10381 gtgaaaggaa cgtttgaaac ggcataaacg gttgtcagcc gcccgaatgg acgtagaatg
    10441 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcgg ttttgcataa
    10501 tattgaaact aaatggcttt atgattttct gaccctggaa aaatgtcgca atttttccca
    10561 ggcagcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga
    10621 acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcggaaca
    10681 aggtaaaata ttccattcgc agatccgcca tctgttgcaa cagttagaga gcaaccttgc
    10741 agaactccgt ggtggcagcg attacgcgca acgcaaaatt aaaatagccg ctgcacactc
    10801 tctttccctc gggctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    10861 aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc aaagtgactg
    10921 tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca ttcgcttatt
    10981 tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac tttttaacct
    11041 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    11101 taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg tctcttcgat
    11161 gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta
    11221 cgccatccaa caagaaattc gcagcgggca gctcgttgta ctgaatcggg acgaactggt
    11281 gatcccgatt caggcctatg cataccgaat gaatacccgc atgaatccag ttgccgaacg
    11341 cttctggcgt gaactgcgcg agctggagat tgtacttagc tgacattctc gagcgtcgca
    11401 ctacgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac
    11461 agcattatct caaggctgat cgccagcgtg tggctgtacg cttctttcag attgacctgc
    11521 aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag
    11581 ctctggagat gtttgtcgct ctggttatgc acttgccact cgggcatgcc tgaaacaagt
    11641 aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct acatattgat
    11701 ttacgcagcg atgacgtttt attgcctgaa atttcaggta aaacagcctg acgtgattgg
    11761 cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg
    11821 caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc
    11881 agtctgagac aggcctgaat cc
//
LOCUS       DAFAVF010000128        11712 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFAVF010000128 DAFAVF010000000
VERSION     DAFAVF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.128"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(227..541)
                     /gene="fliE"
                     /locus_tag="LGK42_003400"
     CDS             complement(227..541)
                     /gene="fliE"
                     /locus_tag="LGK42_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBK2659616.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            756..2414
                     /gene="fliF"
                     /locus_tag="LGK42_003401"
     CDS             756..2414
                     /gene="fliF"
                     /locus_tag="LGK42_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBK2659617.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRVSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QFNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2407..3402
                     /gene="fliG"
                     /locus_tag="LGK42_003402"
     CDS             2407..3402
                     /gene="fliG"
                     /locus_tag="LGK42_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBK2659618.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3395..4081
                     /gene="fliH"
                     /locus_tag="LGK42_003403"
     CDS             3395..4081
                     /gene="fliH"
                     /locus_tag="LGK42_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBK2659619.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4081..5454
                     /gene="fliI"
                     /locus_tag="LGK42_003404"
     CDS             4081..5454
                     /gene="fliI"
                     /locus_tag="LGK42_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBK2659620.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5473..5916
                     /gene="fliJ"
                     /locus_tag="LGK42_003405"
     CDS             5473..5916
                     /gene="fliJ"
                     /locus_tag="LGK42_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBK2659621.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5913..7040
                     /gene="fliK"
                     /locus_tag="LGK42_003406"
     CDS             5913..7040
                     /gene="fliK"
                     /locus_tag="LGK42_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBK2659622.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTMAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7145..7609
                     /gene="fliL"
                     /locus_tag="LGK42_003407"
     CDS             7145..7609
                     /gene="fliL"
                     /locus_tag="LGK42_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBK2659623.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7614..8618
                     /gene="fliM"
                     /locus_tag="LGK42_003408"
     CDS             7614..8618
                     /gene="fliM"
                     /locus_tag="LGK42_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBK2659624.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8615..9028
                     /gene="fliN"
                     /locus_tag="LGK42_003409"
     CDS             8615..9028
                     /gene="fliN"
                     /locus_tag="LGK42_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBK2659625.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9031..9396
                     /gene="fliO"
                     /locus_tag="LGK42_003410"
     CDS             9031..9396
                     /gene="fliO"
                     /locus_tag="LGK42_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBK2659626.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9396..10133
                     /gene="fliP"
                     /locus_tag="LGK42_003411"
     CDS             9396..10133
                     /gene="fliP"
                     /locus_tag="LGK42_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBK2659627.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10143..10412
                     /gene="fliQ"
                     /locus_tag="LGK42_003412"
     CDS             10143..10412
                     /gene="fliQ"
                     /locus_tag="LGK42_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBK2659628.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10421..11206
                     /gene="fliR"
                     /locus_tag="LGK42_003413"
     CDS             10421..11206
                     /gene="fliR"
                     /locus_tag="LGK42_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBK2659629.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11496..>11712
                     /gene="rcsA"
                     /locus_tag="LGK42_003414"
     CDS             11496..>11712
                     /gene="rcsA"
                     /locus_tag="LGK42_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBK2659630.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHII"
BASE COUNT         2789 a         3091 c         3188 g         2644 t
ORIGIN      
        1 attattcaaa cgttactgtt gcgtctgcgg tcgtagcaac atccgacgtg gtgatgagtg
       61 ttaaaagaga cactctgatg ttcagagggg ttatcgcgta tacggatcgt ggatggcaga
      121 aaactttcag aatcaggttt ccattttaaa ccagaaattg agtgagtttg ccccatccat
      181 gccccacgcg gtgagatcgg atgtaattaa caacaggtta caaaacctac acctgcatgc
      241 tcatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
      301 aaactgaggc tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga
      361 gagtgaattt ttctgcctgc gtgcgggcag ctgtttgtgt atcactaatg cgatcgagcg
      421 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg
      481 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
      541 ttctcgtctc ccggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg
      601 cgctaaataa caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat
      661 aatgggagcg tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc
      721 aataacgccg tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa
      781 aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg
      841 gttccgcggc agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc
      901 gcacattatt cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc
      961 aaatgaatat tccttaccgc gtcagcgaag ccagcggcgc tattgaagtt ccggcagata
     1021 aagttcacga gctgcgtctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg
     1081 gtttcgaact actcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
     1141 atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag
     1201 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
     1261 cttctgcatc ggtgacggta aatctgttac ccggccgcgc actcgatgaa gggcaaatta
     1321 gcgccattgt acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc
     1381 tggtggatca gggtggacat ctgttaaccc agttcaacac cagcgggcgc gatcttaatg
     1441 acgctcagtt gaaatatgcc agcgatgtcg aaggccgtat tcagcggcgt attgaagcga
     1501 tcctgtcgcc tattgttggt aacggtaata ttcacgccca ggtcacggcg cagctggact
     1561 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
     1621 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
     1681 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
     1741 cggcaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta
     1801 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
     1861 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc
     1921 cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc
     1981 gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt
     2041 tcaatagcag tgacgaaagc ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg
     2101 atcagttgct ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc
     2161 ggaaagcggt acgcccgcag ttaacacgtc gcgccgaggc gatgaaagct gtacagcaac
     2221 aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac
     2281 aactccaaca acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc
     2341 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
     2401 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
     2461 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aacccttagc
     2521 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggaa
     2581 tttgagcaag aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt
     2641 tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga agatattctc
     2701 gaaactcgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc
     2761 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag
     2821 cgcgcgcagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
     2881 ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta
     2941 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaaca
     3001 gcagccgaaa ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg
     3061 cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat
     3121 ctggtggatg tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
     3181 ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
     3241 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg
     3301 tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact
     3361 ggcgagatgg taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
     3421 ctggacgccg gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga
     3481 agaaaccatc attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat
     3541 gcaggcccat gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga
     3601 gcagggctat caggaaggac tggcccgggg gctggagcaa ggtctggcag aggcgaagtc
     3661 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
     3721 tgatgcgctt gatagtgtga ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg
     3781 tcaggtgatc ggtcagacgc caacagtgga taactcggca ctgatcaaac agatccaaca
     3841 gttgttgcag caagaaccgt tattcagcgg taaaccacag ctgcgcgtgc acccggatga
     3901 tctgcaacgt gtggatgata tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg
     3961 gggcgatccc acactccatc ctggcggctg taaagtctcc gccgatgaag gcgatctcga
     4021 cgccagtgtc gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta
     4081 atgaccacgc gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg
     4141 cagttgcctg cggtacgtcg ctatgggcga ttaacccgcg ctaccgggct ggtgctggaa
     4201 gccaccggat tacaattgcc gctcggcgca acctgtgtca ttgaacgcca gaacggcagc
     4261 gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg
     4321 ccgctggagg aagtggaagg tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg
     4381 gctgaaggac tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt
     4441 ctggacggca gcggtaagcc gctcgatggc ctgccctccc ccgatacgac ggaaaccggt
     4501 gcgctgatta ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac
     4561 accggcgtgc gcccaatcaa tgccctgctt accgttgggc gtgggcagcg tatggggctg
     4621 tttgccgggt ccggcgttgg taaaagtgtg ctgctgggga tgatggcacg ttacacccgc
     4681 gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa agattttatt
     4741 gagaatatcc tcggtgccga agggcgtgca cgctccgtgg tgattgccgc tccggcggat
     4801 gtctctccgc ttctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt
     4861 cgcgatcgtg gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc
     4921 cagcgtgaaa ttgcgctggc gattggcgaa ccacccgcca ccaaaggtta tcccccgtcg
     4981 gtgtttgcca aattaccggc actggtcgag cgtgccggaa atggcattag cggcggcgga
     5041 tcgattaccg cgttttatac cgtgctcacc gagggcgatg accagcagga ccccattgcc
     5101 gactccgcgc gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc
     5161 gggcactatc cggctatcga tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc
     5221 agcgagcaac attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt
     5281 aaccgagatc tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgataaa
     5341 gccatcgccc tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg
     5401 gactgggaag cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg
     5461 agataacggc agatggcaga acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa
     5521 gaggtagagg atgccgcgcg cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa
     5581 gaacagctca aaatgctgat cgattatcag aatgaatatc gcaataacct caacagcgat
     5641 atgagtgccg ggatgaccag caaccgctgg atcaactatc agcagtttat ccagacgctg
     5701 gaaaaagcca ttactcagca tcgccagcaa cttaatcagt ggacgcagaa agttgacatt
     5761 gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg cctggcagac actgcaggaa
     5821 cggcaatcca cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag
     5881 ttcgcccagc gcgccgccat gaggaaacct gaatgattcg cttagcgccc ttaattaccg
     5941 ccgacgttga caccaccaca ttgcctggcg gcaaagccag cgatgctgca caagattttc
     6001 tcgcgttgtt gagcgaagca ttagcaggcg agacaactac cgacaaagcg gccccccagt
     6061 tgctggtggc aacagataag cccacgacaa aaggcgagcc gctggtcagc gagattcttg
     6121 ccgatgcgca acaagcggat ttactgatcc ctgtggatga aacaccgcct gtcatcaacg
     6181 acgaacaatc cacatcaaca ccattaacca ccgctcaaac gatgacgatg gctgcggtgg
     6241 ctggcaacaa tacggcaaaa gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa
     6301 gcctgagcgc cctttttgcg atgttgccgg gttttgacaa tacgcccaaa gtgactgatg
     6361 tgccgtcaac cgtgttaccg gcagagaaac caacgctctt cacaaaactg acttctgcgc
     6421 aactcacaac agcacagcct gatgatgccc ccggcacgcc agctcagcca ttaacaccgc
     6481 tggtagcaga agcccagagt aaagcggaag tcatcagcac accttcaccg gtgaccgctg
     6541 ccgccagccc gctaatcact ccacaccaga cacagccact gcccaccgtc gccgcgcctg
     6601 tgttgagtgc accgctgggt tctcacgaat ggcaacaatc attaagccag catatttcgc
     6661 tgttcacccg ccaggggcaa caaagtgcag agttgcgtct gcacccgcag gatttaggtg
     6721 aagtgcaaat ctccctcaaa gtggatgata accaggcgca aatccagatg gtttcaccgc
     6781 atcaacacgt acgcgccgcc ctggaagcag cgcttccggt actgcgaacg cagctggccg
     6841 aaagtggcat tcagttaggg caaagcaaca tcagtggcga aagctttagt ggtcagcagc
     6901 aggccgcttc ccaacaacag caaagccaac gcacagtaaa ccatgaacct ctggcggggg
     6961 aagacgacga tacgcttccg gttcccgtct ctttacaagg gcgtgtaaca ggcaacagcg
     7021 gcgttgatat tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt
     7081 tgttgcgctc aagacgcagg ataattagcc gataagcagt agcgacacag gaagaccgca
     7141 acacatgact gattacgcga taagcaagaa aagcaagcga tcgctttgga tcccgattct
     7201 ggtattcatt accctcgcgg cctgtgccag cgcaggttac agctactggc attcgcatca
     7261 ggttgccgct gacgacaaag cgcagcaacg cgtcgtaccc tccccggtct tctacgcgct
     7321 ggataccttc acggtcaatt tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct
     7381 gcgcctgaaa gatgaagcta cccgctcgcg gctgagtgag tatttgccgg aagtccgtag
     7441 ccgtttgctg ttactgtttt cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa
     7501 gaaaaacctg attgccgaga ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc
     7561 gaaacaggat gtcaccgacg tgctgtatac cgcttttatt ctgcgataac gacatgggcg
     7621 atagtattct ttctcaagct gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag
     7681 acgaaccgac agccagtgtt agcggcgaaa gtgacattcg tccgtacgat ccgaataccc
     7741 aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag cgctttgccc
     7801 gccattttcg tatggggctg ttcaacctgc tgcgtcgtag cccggatata accgtcgggg
     7861 ccatccgcat tcagccgtac catgaatttg cccgcaacct gccggtgccg accaacctga
     7921 accttatcca tctgaaaccg ctgcgcggca cagggctggt ggtgttttca ccgagtctgg
     7981 tgtttatcgc cgtggataac ctgtttggcg gcgatggacg cttcccgacc aaagtggaag
     8041 gtcgcgagtt tacccatacc gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg
     8101 aaggctatag cgacgcctgg aaggcgatta atccgctgga agttgagtac gtgcgttcgg
     8161 aaatgcaggt gaaatttacc aatatcacca cctcgccgaa cgacattgtg gttaacacgc
     8221 cgttccatgt ggagattggc aacctgaccg gcgaatttaa tatctgtctg ccattcagca
     8281 tgatcgagcc gctacgggaa ttgttggtta acccgccgct ggaaaactcg cgtaatgaag
     8341 atcagaactg gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg
     8401 ccaactttgc cgatatctcg ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg
     8461 tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt tgacggcgtc ccggtgctga
     8521 ccagtcagta cggcactctc aacggtcagt atgcgttacg gatagaacat ttgattaacc
     8581 cgattttaaa ttctctgaac gaggaacagc ccaaatgagt gacatgaata atccggccga
     8641 tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac
     8701 cagcagcaaa agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg
     8761 aacgttgcag gatatcgacc tgataatgga tattccggtc aagctgaccg tcgagctggg
     8821 ccgtacgcgg atgaccatca aagagctgtt gcgtctgacg caagggtccg tcgtggcgct
     8881 ggacggtctg gcgggcgaac cactggatat tctgatcaac ggttatttaa tcgcccaggg
     8941 cgaagtggtg gtcgttgccg ataaatatgg cgtgcggatc accgatatca ttactccgtc
     9001 tgagcgaatg cgtcgcctga gccgttagtg atgaataacc acgctactgt gcaatcttcc
     9061 acgccggttt ctgctgcgcc actgctgcag gtcagcggcg cactcatcgc cattattgcc
     9121 ctgatcctcg ctgctgcctg gctggtaaaa cggttgggat ttgccccgaa acgcactggc
     9181 gttaacggtc tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg ggttgtggtg
     9241 gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg cagggcaaat caatctgctg
     9301 cataaacttc ccccttcagc accaacagaa gagataccgc agaccgattt tcagtcggtc
     9361 atgaaaaatt tgcttaagcg tagtgggaga tcctgatgcg tcgtttgttg tctgtcgcac
     9421 ctgtccttct ctggctggtt acgcccctcg ccttcgcgca actgccgggt atcaccagcc
     9481 agccgctgcc tggcggtgga caaagctggt cgctcccggt gcagacgctg gtgttcatca
     9541 cctcgttgac gtttattccg gcaattttac tgatgatgac cagtttcacc cgcatcatca
     9601 ttgtttttgg tttattgcgt aacgcgctgg gaacgccctc cgcgccacct aaccaggtat
     9661 tgctggggct ggcactattt ttgacctttt ttattatgtc accggtgatc gataaaattt
     9721 atgtcgatgc gtaccagcca ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa
     9781 aaggggcgca gccgctacgt gagtttatgc tgcgtcagac ccgtgaggca gatttagggt
     9841 tgtttgccag actagcgaat accggcccgt tgcagggacc tgaagccgtg ccgatgcgca
     9901 ttttgctccc ggcctacgtg accagcgagt tgaaaaccgc atttcagata ggcttcacga
     9961 ttttcattcc ttttttgatt atcgacctgg tgattgccag cgtgttgatg gcattgggga
    10021 tgatgatggt cccccccgcc accattgctc tgccctttaa actgatgctg tttgtactgg
    10081 tggatggctg gcaattgctg gtcggttcgc tggcgcagag cttttacagc tagagaggca
    10141 aaatgacacc tgaatcggtc atgatgatgg ggactgaagc gatgaaagtc gcgctggcac
    10201 tggctgcccc gctgttgctg gtggcgctgg tcacgggcct tatcatcagt attttgcagg
    10261 ccgccacgca gatcaacgaa atgacgctgt cgtttattcc aaaaatcatc gccgtattta
    10321 ttgccattat tattgccgga ccgtggatgc tcaatctgtt gctggattac gtccgcacct
    10381 tgttcactaa cctgccgtat atcatcgggt agcccgtaca atgatgcagg tgacaagcga
    10441 tcaatggctt tcctggctaa gtctgtactt ctggccgtta ctgcgcgtgc tggcgctcat
    10501 ttctaccgcg ccaattctga gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc
    10561 aatgatgatc acgttcgcta ttgccccatc attacctgcc aacgatgttc ctgttttttc
    10621 gttctttgct ctgtggctgg ccgtgcagca gatcctgatc ggcattgcgc ttggttttac
    10681 catgcaattt gcctttgccg ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg
    10741 gctgtcattt gcgacgtttg tcgatccggc cagccatctc aatatgcccg ttttagcgcg
    10801 tatcatggat atgctggcgt tactgctgtt cctgacattt aacggtcatt tatggttgat
    10861 ttcactgctg gtcgatacct ttcacaccct gccgattggt ggcgaaccgt tgaacagcaa
    10921 tgcgtttctg gcactcacca aagcagggag tttgattttc cttaacgggc tgatgctggc
    10981 gttaccgctc attactctgc tgctgacact gaatctggca ttaggtttac ttaatcgtat
    11041 ggccccgcaa ttatccattt ttgttattgg atttccatta actctgactg tcggcatctc
    11101 tttaatggcg gcattaatgc cgttaattgc gcctttttgc gaacatttat tcagtgaaat
    11161 ttttaatttg ctggctgata ttattagtga attgccatta atataattcc gtaacgttta
    11221 tcatgttatc ctaaggatta tccgaaaaat aatacctacg aacatcttcc aggatactcc
    11281 tgcagcgaaa tatttgtttt aagctcactc acatatcgca acatttactt tactttaaga
    11341 caattccagg caaattatac aacactttac gggatagtaa gtccgcctga aaaatcgcga
    11401 gagtggcgca ttaggtgacc catgttgttc cgtttagtca tgatgaaata ttcaggtaag
    11461 gggaattatc gttacgcatt gagtgagggt atgccatgtc aacgattatt atggatttat
    11521 gtagttacac ccgactaggt ttaaccgggt atctgttgag tagaggggtt aaaaaaagag
    11581 aaatcaacga cattgaaacc gttgatgacc ttgccatagc ttgtgattca cagcgccctt
    11641 cagtggtgtt tattaatgag gactgtttca tccatgatgc ttctaacagt cagcgcatca
    11701 agcacatcat ta
//
LOCUS       DAFAVF010000129        11660 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFAVF010000129 DAFAVF010000000
VERSION     DAFAVF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.129"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..100)
                     /locus_tag="LGK42_003415"
     CDS             complement(<1..100)
                     /locus_tag="LGK42_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HBK2659631.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGR"
     gene            complement(363..1472)
                     /gene="apbC"
                     /locus_tag="LGK42_003416"
     CDS             complement(363..1472)
                     /gene="apbC"
                     /locus_tag="LGK42_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBK2659632.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1604..3637
                     /gene="metG"
                     /locus_tag="LGK42_003417"
     CDS             1604..3637
                     /gene="metG"
                     /locus_tag="LGK42_003417"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK2659633.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3778..7572
                     /locus_tag="LGK42_003418"
     CDS             3778..7572
                     /locus_tag="LGK42_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBK2659634.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKVNSLQPSQTVTGSLKVADLSTIIQEQLSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPRLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7582..11214
                     /locus_tag="LGK42_003419"
     CDS             7582..11214
                     /locus_tag="LGK42_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBK2659635.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     MMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATEPARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPIARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     IFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPVKDNWAFLALSLFGDESTARDLTTQILGWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     AYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLARCTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11275..11592
                     /locus_tag="LGK42_003420"
     CDS             11275..11592
                     /locus_tag="LGK42_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659636.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3047 a         2971 c         2881 g         2761 t
ORIGIN      
        1 tcctgccatt aatcgctaat gtggcagtct ctgaagagaa tgcgggagaa gccaatccat
       61 aggcaagaaa aataattcct gacagccaat acttattcat tgaacgttat ccctgtagta
      121 aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc cactacacag
      181 ccacgaagga tgataatgaa gcattgcctg tatgatcaat cgactttgta gagtttcgga
      241 cgaaggtccg cagaatattc gcagtattaa ataagtgttc attaatgcgg caaaaactaa
      301 tacaccgcat caatgtaaca tctctgcggt gtatgaagaa taaccagaat ggttaatagg
      361 cgttagaccg cgcggaagga aatctcgcct ggaatgactt caccctgcca gtagagctga
      421 gctgcaacgc ggtcagccag ctggcggtag atagcggtaa attcgctctc cggacggctg
      481 ataaccgttg gcgtgccttt atcgagatcc tcacgtaagg agatgtgtaa cggcatctgc
      541 cccagcagct gtgtgtggta tttttcagcc agtttctcag caccaccggt accgaaaatc
      601 ggttcatggt gaccacagtt actgcaaata tgcacactca tgttctcgac aataccaagc
      661 accggaacct cgactttctc gaacatcaca atgccttttt tcgcatcgat caacgcgatg
      721 tcctgcgggg tggtgaccac gactgcacca gttactggaa tgttttgcgc cagcgtcaat
      781 tgaatgtcac cggtgcctgg cggcatatca agcaccagat agtcgagatc cggccacaag
      841 gtttcctgca acatctgcat taacgccttg ctcgccatcg gtccacgcca caccatcgca
      901 ttgtcgtcag tgaccagata accaattgaa ttggttgcca ggccatgaga cataatcggt
      961 gccatgtggg taccgtccgg cgacgttgga cgctgatttt ctgcgcccag catggtggga
     1021 attgatgggc cgtagatatc ggcgtccaga atgccgactt tcgccccttc cgccgccagt
     1081 gccagcgcca ggtttaccgc cgtggaggat ttacccacgc cgcctttacc ggagctgacc
     1141 gcaatgatgt tcttcacacc attaataccc ggctgattct tcacgcgttt taaggtggcg
     1201 atattgtgcg acagtttcca gtcgatagct ttcgccccgg tgatacgcag cagttcggcg
     1261 ctacattgct cttttaactc ttcaaacgcg ctatgccaga cgaatggcat aaccagttcc
     1321 acatgcaacg tgtcgtccat ccaggcgaca tggtgcaacg ctttcagcgt ggtgaggtta
     1381 tgcttcaggg ttgggtgctg aaaattggcc agtgtcccgg cgaccattgc gcgcaaggct
     1441 tctggtgatt tggcctggga ttgttcgttc atcccgactc cttttttgta tagataaacc
     1501 atcagctgat agtttacctg aagaatatag agaagtactt acttaacatt ttcccatttg
     1561 gtactatcta accccttttc actattaaga agtaatgcct actatgactc aagtcgcgaa
     1621 gaaaattctg gtgacgtgcg cactgccgta cgctaacggc tcaatccacc tcggccatat
     1681 gctggagcac atccaggctg atgtctgggt ccgttaccag cgaatgcgcg gccacgaggt
     1741 caacttcatc tgcgccgacg atgcccacgg tacaccgatc atgctgaaag ctcagcagct
     1801 tggtatcacc ccggagcaga tgattggcga aatgagtcag gagcatcaga ctgatttcgc
     1861 aggctttaac atcagctatg acaactatca ctcgacgcac agcgaagaga accgccagtt
     1921 gtcagaactt atctactctc gcctgaaaga gaacggtttt attaaaaacc gcaccatctc
     1981 tcagctgtac gatccggaaa aaggcatgtt cctgccggac cgttttgtga aaggcacctg
     2041 cccgaaatgt aaatccccgg atcaatacgg cgataactgc gaagtctgcg gcgcgaccta
     2101 cagcccgact gaattgatcg agccgaaatc ggtggtttct ggcgctacgc cggtaatgcg
     2161 tgattctgaa cacttcttct ttgatctgcc ctctttcagc gaaatgttgc aggcatggac
     2221 ccgcagcggt gcgttgcagg agcaggtggc aaataaaatg caggagtggt ttgaatccgg
     2281 cctgcaacag tgggatatct cccgcgacgc gccatacttt ggttttgaaa ttccgaacgc
     2341 gccgggcaaa tatttctacg tctggctgga cgcaccgatt ggctacatgg gttctttcaa
     2401 gaatctgtgc gacaagcgcg gcgacagcgt aagcttcgat gaatactgga agaaagactc
     2461 caccgccgag ctgtaccact tcatcggtaa agatattgtt tacttccaca gcctgttctg
     2521 gcctgccatg ctggaaggca gcaacttccg caagccgacc aacctgtttg ttcatggcta
     2581 tgtgacggtg aacggcgcaa agatgtccaa gtctcgcggc acctttatta aagccagcac
     2641 ctggctgaat cattttgacg ctgacagcct gcgttactac tacactgcga aactctcttc
     2701 gcgcattgat gatatcgatc tcaacctgga ggatttcgtt cagcgtgtga atgccgatat
     2761 cgtgaacaaa gtggttaacc tggcctctcg taatgcgggc tttatcaaca agcgttttga
     2821 cggcgtgctg gcaagcgaat tggctgaccc gcagttgtac aaaaccttca ctgatgccgc
     2881 tgaagtgatt ggtgaagcgt gggaaagccg cgaatttggt aaagcagtgc gcgaaatcat
     2941 ggcgctggct gatctggcta accgctatgt cgacgagcag gctccgtggg tggtggcaaa
     3001 acaggaaggc cgcgatgccg acctgcaggc aatttgctca atgggcataa acctgttccg
     3061 cgttctgatg acttacctga agccggtact gccgaaactg accgagcgcg cagaagcatt
     3121 cctcaataca gaactgacct gggatggtat ccagcaaccg ctgctgggcc acaaagtgaa
     3181 tccgttcaaa gcgctgtata accgcatcga tatgaagcag gttgaagcac tggtggaagc
     3241 gtctaaagaa gaagtgaaag cagctgccgc gccagttact ggcccactgg cagatgatcc
     3301 gattcaggaa accatcacct ttgacgactt cgcaaaagtt gacctgcgcg ttgcgctgat
     3361 cgaaaacgca gagtttgttg aaggttctga caaactgctg cgcctgacac tggatctcgg
     3421 cggtgaaaaa cgcaatgtct tctccggtat tcgttctgcc tacccggatc cgcaggcact
     3481 gatcgggcgt cacaccatta tggtggccaa cctggcaccg cgtaaaatgc gctttggtat
     3541 ctccgaaggc atggtaatgg ctgctggtcc tggcgggaaa gatatcttcc tgttaagccc
     3601 ggatgctggt gctaaaccag gccatcaggt gaaataatcc ccctttaagg cgctgcatcg
     3661 acagcgcctt ttctttataa attcctaaag ttgttttctt gcgattttgt ctctctctaa
     3721 cccgcataaa tactggtagc atctgcattc aactggataa aatcacaggg atgcagaatg
     3781 agacacttta tctatcagga cgaaaaatca cataaattct gggcagttga gcaacaggga
     3841 aacgagttgc atatcagttg gggaaaagtt ggcaccaaag ggcaaagcca gataaaaagt
     3901 ttttcagatg ctgcggcagc ggcaaaagcg gagcttaagc tgattgcgga gaaggtgaag
     3961 aaggggtatg tggagcaagc gaaggttaat tctttgcaac cttcccaaac ggtaacgggc
     4021 tctctcaagg tagcggactt atccaccatt attcaggaac aactctcttt cgtagcagaa
     4081 acccgtgcgc ctgacaaaaa tacagatgct gttttaccgt ggctggcgaa agatattgct
     4141 gtcgtttttc cgcccgaagt tgtacacacc acgttaagtc atcgccgctt tcccggagtt
     4201 cctgttcagc aagcagacaa attgacccaa ttacgtcgct tagcctgtag tgtgtcgcaa
     4261 cgggataata aaacagccac atttgacttc agcgcctgtt ctttagaatg gcaaaacacc
     4321 gtcgcccagg cgatcagtca gatcgacggc ctgaaaacaa cacagctacc atcaccagta
     4381 atggctgtac tcacggcact tgaaatgaaa tgcacaagat ataaagtgcg tgaggatgtt
     4441 atggatcaga tcgtccagga aggcggtctg gaatatgcta ctgatgtaat aatacacctt
     4501 caacagattg atattaaatg ggattatgcg aataatgtca ttattattct accgtctggc
     4561 attgcacctg actacttgga gcaatattcc agatttgaat tacgcctacg taaacattta
     4621 tcactggcgg aagagtctct ctggcaaaaa tgtgcacaaa aacttattgc cgcaattcca
     4681 catattccag aatggcggca accattaatt gctttgttat tacccgaaaa accagaaatt
     4741 gcacatgaaa ttgcccagcg tctactgggg caaaaaaaat taccctcgct tgagtggtta
     4801 aaaatagtgg cgactgatga gcacattctt gcctcattag aaaaatatca cgagccatat
     4861 gccatttttg atgattacta ttgtggtgcg atatggtcag ccaccgtatt acaggagcaa
     4921 ggtgttgcag ccctgccccg atttgctccc tatgccgcaa gtgactactg cgccgatgtg
     4981 ttgcgtcata tcaatcatcc gttcgcatta acactgctta tacgtgtagc cgggcatact
     5041 aaacgctgtc acgatcggat gacgaaagcc attgctgcgt tcccacatgc agcaatggcg
     5101 gcactgacgg aacttcttgg gcaaaaagaa gagaacagtt ggcgcattat gctaatgaca
     5161 atgcttatct cacaaccagc actggcagaa caggtcattc cctggctctc gacacccgca
     5221 gttgccgtac tgaaatcatg ccagcaacaa ctgacacagc cctcaaacca tgccagcgcc
     5281 gatctactgc cagccgtagt agtctcccct ccctggcttt cgaaaaagaa aaaatcgccg
     5341 attccggtgc tggatttagc gccattaggc attgagccaa tctgttatct gacagaagaa
     5401 atcagtaatc aacttttggc gaaatatatc tggtattcaa aacacatcac ggttagccat
     5461 gaagaaagta ctaccaacct gttggcaagg atgggttttc aacgacggat cgctggtaca
     5521 tatattaaag ctcccgaagc ggtagttgag gcatggctaa atgaagatta ttcaacctta
     5581 ctaagtgaat ttaaggtgtt tcattcacct accgggcatt attggcagtt ggggattttg
     5641 acaacattgc cgctggagaa agcagtaaaa gcatggaatg cccttaccct atctccacat
     5701 accgataccg aatacgccat gttacatttt ggactcaaag ggttacctag gttagtaaac
     5761 tcacttgcac gctatccaca agaagccttg cccatcacga attacttcgc agcgagtgag
     5821 ctggctcctg ccgtcgcccg tgccttcaac aaactgaaaa cgctacgcga aaacgcccgt
     5881 agctggctgt tgaaataccc ggaacatgcc cttaccggcc tgctgcctgc ggcgctcggc
     5941 aaagccggtg aagcacagga taacgcccgc gctgccttgc gtatgcttac cgaaaacggt
     6001 catcagccat tactgcaaga aatcgcccga cgttataacc agccggaagt aaccgatgcg
     6061 gtgaacgctc tgcttgcgct cgatccctta gataatcacc cgacaaaaat ccccactctt
     6121 ccggcctttt atcagccatc gctctggacg cgcccggtat taaaagcaaa tgcccaatca
     6181 ctgccagata gcgccctcct ccacctcggt gaaatgctcc gcttccctca ggaagaggct
     6241 ctgtatccgg gattattgca ggtgaaagac gtctgttccg ccgactcact ggcgggattt
     6301 gcctgggatc tgtttaccgc ctggcagacc gctggcgcgc cgtcgaaaga gagttgggcg
     6361 ttcactgcgt taggcgttct cggtaacgat gacaccgccc gcaaactgac gccattaata
     6421 cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg ttgggttgga tattctcgct
     6481 gctatcggta gtgatatcgc ccttatgcag cttaacggca tcgcccagaa actgaaattc
     6541 aaagcattac aggagcgggc aaaagaaaaa attgccgaca ttgccgagag ccgcgaactc
     6601 acggtggcgg agcttgaaga tcggttagca ccggatctcg gtctggatga taacggttcg
     6661 ctgctgctgg attttggccc acggcagttc accgtcagct ttgatgaaac cttaaaaccg
     6721 tttgtgcgtg atgtttccgg cagccgcctg aaagacctgc ccaaaccgaa caaaagcgat
     6781 gatgaatcgc ggtcgaacga tgcggttaac cgctacaaat tgctgaaaaa agatgcgcgt
     6841 accgtcgccg cccagcaggt agcaaggctg gaatccgcca tgtgcctgcg ccgccgctgg
     6901 tcgccggaaa acttccagct cttcctggtt gagcatccgc tggttcgcca cttaacccgc
     6961 cgtctgattt ggggcgttta tagcgccgaa aaccagctac tggcttgctt tcgcgtagca
     7021 gaagataaca gctacagcac cgctgacgat gatcttttca ccctgccgga aggcgatatc
     7081 tctatcggca ctcctcacgt tctggaaata tcaccaacgg atgctgccgc ctttggtcag
     7141 ctttttgccg actacgaact gctaccaccg ttccgccagc tcgaccgtaa cagctacgcc
     7201 ctgacagaag ccgagcgcaa tgccagtgaa ctgacccgct gggcaggcag aaaatgcccg
     7261 agtggtcggg tcatggggct ggcgaataaa ggctggataa agggcgaacc acaggatgga
     7321 ggctggatcg gatggatgat caaacctttg ggtcgctggt cgttaatcat ggaaatcgac
     7381 gaaggttttg cggtgggtat gtcaccagcc gaacttagcg ctgagcagct cttaagcaag
     7441 ctgtggttat gggaaggcaa agcggaaagc tatggctggg ggagtaattc aacacaggaa
     7501 gcgcagttct ccgtactcga tgccatcacc gccagcgagc taattaacga tattgaagcc
     7561 ctgtttgaat aaggaaagag catggacaag gaattaccgt ggctggcgga taacgcccaa
     7621 ctggaactga aatataaaaa aggcaaaaca ccactcagtc atcgcaaatg gccgggcgaa
     7681 ccggtgcctg ttatcactga aagcatcatt cagacactgg gtgatgaatt gctacaaaaa
     7741 gctgagaaga aaaaaaacat tgtctggcgt tatgaaaatt tttcactgga gtggcagtcc
     7801 gccatcacgc aggccatcaa cttgatcggc gaacacaaac cctcaatccc ggcccggaca
     7861 atggcggcgc tagcctgtat cgcgcaaaat gacagccaac agttgctcga cgaaatcgtc
     7921 caacaagagg ggctggaata tgcgactgag gtggtgattg cacgccagtt tattgcgcgg
     7981 tgttatgaga gtgatcctct ggtagtgaca ttgcagtatc aggacgagga ttatggctat
     8041 ggttatcgct cagaaaccta taacgaattc gatctccgac tgcgtaagca tctctctctg
     8101 gcagaggaaa gctgctggca gcgttgcgcc gacaaactca ttgccgcact accaggaata
     8161 accaaagttc gccgcccttt tattgcgctg atcctcccgg aaaaaccaga aatagccaat
     8221 gagttggtag gccttgaatg cccgcgaact cattttcatt ctaaggagtg gttaaaagtt
     8281 gttgctaatg accccacagc ggtgagaaaa ctcgagcact actggagcca ggatatattt
     8341 agcgatcgag aagccagcta catgtcgcat gaaaaccact tcggctacgc ggcctgcgcc
     8401 gcccttttgc gcgaacaagg actggcagcc attccgcgcc tcgcgatgta tgcccataaa
     8461 gaagattgcg gcagtctgct ggtacaaatt aaccatccgc aagtcatccg caccttgcta
     8521 ctggtggctg ataaaaacaa acccagcctg caacgtgtag ctaaatacca taaaaacttc
     8581 ccccatgcga cgctcgccgc actggcagaa ctgctggcgt taacagaacc accagcccgc
     8641 ccaggttatc caatcatcga agacaaaaag ctgcctgcac agcaaaaagc acgcgatgaa
     8701 tactggcgta cgctgttaca aacgctgatg gcatcgcagc cacaactggc agaagagatg
     8761 atgcagtggt taagtactca agccagggca gtgctgaata gttatttatc ggcaccgccc
     8821 aaaccggtta ttgatagtac cgataacagc aatctgcctg aaatcctcgt ttcaccaccg
     8881 tggcgtagta agaaaaaaat gacagctcca cgtcttgatt tggcaccgct cgaattaacg
     8941 ccgcaagttt actggcaacc tggcgaacga gagaggcttg ccgccactga gcctgcccgt
     9001 tatttcagca cggaatctct tgcggaacgc atggaacaaa aaagtgggcg agttgtatta
     9061 caggaactgg gttttgggga tgatgtatgg ctgtttttga attatatact ccccggaaaa
     9121 ctggatgctg cacgcaattc actcattgtt cagtggcatt actaccaggg gcgggttgaa
     9181 gagatcctga atggctggaa ctccccggaa gcacaattag cagaacaggc gctccgcagc
     9241 ggtcacatag aagcgttaat taacatatgg gaaaatgaca actactcacg ttatcgtccg
     9301 gaaaagagtg tctggaacct gtatttattg gcacagttgc cgcgtgagat ggctttgacc
     9361 ttctggctgc gtatcaatga gaaaaaacat ctgttcgcgg gtgaggacta ttttctcagt
     9421 atcctcgggt tggatgcgct accaggtctg atgttggctt tttcacatcg tccaaaagaa
     9481 acatttccgt taattttaaa tttcggcgca acagaactgg ccctgcccat tgcccgcgtc
     9541 tggcaccgtt ttgcgggcca gcgtaatctg gctcgccagt ggattttaca atggccggaa
     9601 catacggcta ctgcacttat ccctcttatc ttcaccaaat ctagcgataa aagcgaagct
     9661 gcattacttg ccctgcgttt actttacgaa catgggcatg gcgaattgct acaaactgta
     9721 gccaatcgtt ggcagcgtac agatctatgg cctgccctgg aacaattact taaacagagt
     9781 ccaatagaga tttacccggc acgcattcca aaagcccctg atttctggca tccgcaaatg
     9841 tggtccaggc cgcgccttat cactaataat caacctgtta ccgatgacgc tctggaaatt
     9901 atcggcgaaa tgctgcgctt tacccagggg ggacgttttt atagcgggct ggaacaactg
     9961 aaaacgttct gccagccaca aacgctggca gcttttgcct gggatctctt cactgcgtgg
    10021 caacaagctg gtgcccccgt aaaagacaac tgggcatttc tggcgttaag tctctttggt
    10081 gacgaaagca cggcacggga tctaacgaca cagatcctcg gctggccaca agaaggcaaa
    10141 tctgcccgtg ctgtcagtgg cctgaacatc cttaccctga tgaataatga tatggcgctg
    10201 atacagctgc atcatatatc gcaacgggcg aaatcccgcc ccttacgtga taacgcggcg
    10261 gaatttcttc aggtggtcgc agaaaatcgc gggctaagcc aggaagagct agcggacaga
    10321 ttagtcccaa ccctgggcct tgatgatccg caggcgttga gttttgattt tggtccccgg
    10381 cagtttaccg ttcgcttcga tgaaaacctc aacccggtta tctttgatca gcaaaacgtt
    10441 cgccagaaaa gcgttccccg gttgcgcgcc gatgacgatc aactgaaagc gcccgaggca
    10501 ctggcccgac taaaagggct aaaaaaagat gccactcagg tgagcaaaaa cctgctcccg
    10561 cgtcttgaaa cggccctacg taccacccga cgctggtcgc tggcagattt tcattctctg
    10621 tttgttaatc atccctttac ccgtctggtt acccagcgat taatatgggg ggcttatccg
    10681 gcaaatgaac cgcgttgttt actcaacgcc tttcgtgtgg ccgcagaggg ggagttctgc
    10741 aatgcgcaag atgagccaat tgacctgcct gcggacgctc tgattggcat tgcccacccg
    10801 ttagaaatga cagtagaaat gcgcagtgaa tttgcacagc tttttgccga ttacgaaatt
    10861 atgccgcctt ttcgccagtt ggcgcggtgc acggtgctgc tcacacctga tgagtcaacc
    10921 agtaacagcc tgactcgctg ggaaggtaaa tccgctaccg ttgggcagct tatgggaatg
    10981 cgatacaaag gctgggagtc aggctatgag gatgcatttg tctatgacct gggcgagtac
    11041 cggctggtcc ttaagttttc acccggtttt aaccactaca atgttgatag caaagcgcta
    11101 atgagcttcc gttctcttcg agtgtaccgt gacaataaat ccgtcacttt tgccgaactt
    11161 gatgtgtttg atttgagtga ggcgttaagc gcacctgacg tcattttcca ttaaaacaca
    11221 gcgggcagtg atgcgactgc ccgttatcta cacgacttac cagcggggaa agcgatgatc
    11281 gtgcaaaaag agctagttgc tatttacgat tatgaggtcc ctgtacctga agatccgttt
    11341 tccttcagac ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg
    11401 gagcgattcc ggctacatcc aacatttcat caacgtgatc gagaagatgc tgacccgcta
    11461 ataaatgatg cgttgattta tataagagat gagtgtattg atgagcggaa attacgaggt
    11521 gaatcacctg aaactgtaat agcaattttt aatcgtgaac tacagaatat attcaaccaa
    11581 gaaatagaat aatatactct agataattaa agtcgcagca catgcaactt gaagtatgac
    11641 gagtatagct ttcttaaagg
//
LOCUS       DAFAVF010000130        11499 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFAVF010000130 DAFAVF010000000
VERSION     DAFAVF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.130"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            344..1210
                     /gene="folD"
                     /locus_tag="LGK42_003421"
     CDS             344..1210
                     /gene="folD"
                     /locus_tag="LGK42_003421"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBK2659637.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1212..1424
                     /gene="ybcJ"
                     /locus_tag="LGK42_003422"
     CDS             1212..1424
                     /gene="ybcJ"
                     /locus_tag="LGK42_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBK2659638.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1532..2053
                     /locus_tag="LGK42_003423"
     CDS             1532..2053
                     /locus_tag="LGK42_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2659639.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2089..3474)
                     /gene="cysS"
                     /locus_tag="LGK42_003424"
     CDS             complement(2089..3474)
                     /gene="cysS"
                     /locus_tag="LGK42_003424"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK2659640.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3648..4142
                     /gene="ppiB"
                     /locus_tag="LGK42_003425"
     CDS             3648..4142
                     /gene="ppiB"
                     /locus_tag="LGK42_003425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBK2659641.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4145..4867
                     /gene="lpxH"
                     /locus_tag="LGK42_003426"
     CDS             4145..4867
                     /gene="lpxH"
                     /locus_tag="LGK42_003426"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBK2659642.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4985..5494
                     /gene="purE"
                     /locus_tag="LGK42_003427"
     CDS             4985..5494
                     /gene="purE"
                     /locus_tag="LGK42_003427"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK2659643.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5491..6558
                     /gene="purK"
                     /locus_tag="LGK42_003428"
     CDS             5491..6558
                     /gene="purK"
                     /locus_tag="LGK42_003428"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK2659644.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6695..7588)
                     /locus_tag="LGK42_003429"
     CDS             complement(6695..7588)
                     /locus_tag="LGK42_003429"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK2659645.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKKVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEDTLAGEAGTCISL"
     gene            complement(7585..8400)
                     /locus_tag="LGK42_003430"
     CDS             complement(7585..8400)
                     /locus_tag="LGK42_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBK2659646.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8411..9670)
                     /locus_tag="LGK42_003431"
     CDS             complement(8411..9670)
                     /locus_tag="LGK42_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBK2659647.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9680..11347)
                     /locus_tag="LGK42_003432"
     CDS             complement(9680..11347)
                     /locus_tag="LGK42_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBK2659648.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2573 a         3260 c         3031 g         2635 t
ORIGIN      
        1 atagagagcc ccgttagatg aatacttccg cgcaaaatat attcaacaca aatatagacc
       61 tgaagcggta aattaccagg ctgaaaattc tttttatatt gtcaggtatt tcttaaatta
      121 tcttaatcct taaacaagga aataaatcag ttccagattt acaacgccat catggacgaa
      181 aaatgaagct ttcaatctca gcgacctcag cgacggtgcg cctaaccttc gcaagaggtc
      241 gcttcacgcg ataaatctga aacgaaacct gacagcgcgc cccgcttctg acaaaatagg
      301 cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag caaagattat
      361 tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag ttcaggcgcg
      421 tattgcagcc ggactgcggg caccaggact ggccgttgtg ctggtgggta gtaaccctgc
      481 atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt tcgtctcccg
      541 ctcttatgac ctcccggaaa ccaccagcga agcggagctg ttggagctta tcgatgcgct
      601 gaatgccgac aacaccatcg atggcattct ggttcaactg ccgttaccgg cgggtattga
      661 taacgtcaaa gtgctggaac gtattcatcc ggacaaagac gtggacggtt tccatcctta
      721 taacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc cgcgcggtat
      781 cgtcacgctg cttgagcgtt acaacattga taccttcggc ctcaacgccg tggtgattgg
      841 cgcatcgaat atcgtcggtc gtccaatgag catggaactg ttgctcgcag gttgcaccac
      901 taccgtgact caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg ccgatctgtt
      961 gatcgttgcc gtcggcaagc cgggctttat tcctggtgac tggatcaaag aaggcgcaat
     1021 tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg acgtcgtgtt
     1081 tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt cccggcggcg ttggcccgat
     1141 gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc atgatccaca
     1201 ggatgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag ctgtgcgact
     1261 tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg attgccgaag
     1321 gccaggtgaa agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc gtcgccggtc
     1381 agacagtgag ttttgcaggt cacagcgtac aggttgttgc ctgatccctt tcccggcctg
     1441 tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc atccatgctg
     1501 atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc acgccgctgt
     1561 tcccctatgc attggtttag ggttgggttc gaaagtcatc cctccgcgtc tgttatttgc
     1621 cggaatcatc ctggcgatgc tgcccgacgc cgacgtattg tcgtttaaat ttggcgttgc
     1681 ttacggcaat gtttttggtc atcgcgggtt tacccattcg ctggtgtttg cgtttgttgt
     1741 cccgctgtta tgtgtgctga ttggacgccg atggttcagg gctgggctga ttcgctgctg
     1801 gctgttttta accgtctcgt tgctatcgca cagcttgctg gattcggtaa ccactggcgg
     1861 taagggcgtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc cctggcaggt
     1921 gattaaagtc gcgccgtttg cattgtctcg ttacaccacg ccgtacgggc atcaggtgat
     1981 aatttctgaa ttgatgtggg tgtggttgcc ggggatggtg cttatgggaa tgttgtggtg
     2041 gcgcaggcga taaccggatg cgaaaactcg catccggcaa cagcgcaatt acttacgacg
     2101 ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa gacggtcacg
     2161 cgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct gttgaattaa
     2221 cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca ggaacgcttc
     2281 cggttcttgc tccagcaggc ccaatacagc ggaaagttta cgcaggtgag atgccattgc
     2341 attcgccgct gccatatctt ctgctttcag acggtttact tcacgcgcca tatcaaacag
     2401 tacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa agcgcgcttc
     2461 aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg cagtgtagag
     2521 acgctccagc gccgcacgcg cctgcttcag gttctcttcg ctgtagttca actggctgcg
     2581 atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt tcagcacatc
     2641 gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa ccatcaccat
     2701 ccccgagtgc atccagtagt tcacatactg gccatcatgg gcacaggtgg actgcgcgat
     2761 ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat caaagtggtt
     2821 acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc cagcctggac ggcccgcgcc
     2881 ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa cgaagtccat
     2941 cgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcacct gcctgcagct ggtcgagatc
     3001 ctgacgcgac agcacgccat atgtcggatc ggtcgggacg tcgaacatca cgtcgccgtt
     3061 gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa taatttctgc
     3121 gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag catcaaaatc
     3181 tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc cattttcatt
     3241 ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact tcagtttata
     3301 gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac gcccgtgacc
     3361 gatatgacag agatcgtaaa cggtgattcc acacacatac atgccgactt ccccggcgtg
     3421 aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta gcatcgaaga
     3481 ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt cccgcgcgaa
     3541 tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca ccctatatat
     3601 gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg gttactttcc
     3661 acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct gaaacagtta
     3721 aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc caccgtgtta
     3781 tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa aaagccacca
     3841 aagaaccgat caaaaacgaa gccaacaacg gtctgaaaaa tacccgtggt acgctggcaa
     3901 tggcccgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac gtggttgata
     3961 acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc gtgtttgctg
     4021 aagtggttga aggcatggac gtggtagaca aaatcaaagg tgttgcaacc ggtcgtagcg
     4081 gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc gttagcgagt
     4141 aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac cggcgatcac
     4201 cgccggtttt ctgcgttttt tagcggggga agcccgcaag gccgacgcgc tgtatattct
     4261 tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc atcgccagat
     4321 ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca ttcatggcaa
     4381 ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc ggcatgacat tattgccgga
     4441 agaaaaggtg ctcgaacttt atggtcgccg ggtgttgatt atgcatggcg acacgctgtg
     4501 caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct ggctgcaaat
     4561 gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa tgcgagcgaa
     4621 cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc aaaacgcggt
     4681 agtcagtgcg atggaaaaac atcaggtgca atggctgatc cacggacata cccatcgccc
     4741 ggcggtacat gaacttatcg ccaaccagca acctgctttt cgcgtggtac tcggtgcctg
     4801 gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctga ttcattttcc
     4861 gttttaaaaa acccgcaact ttgctgattt cacagccacg caaccgtttt ccttgctctc
     4921 tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag gagttttaag
     4981 acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt ccaaaagcga
     5041 ctgggctacc atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc cgcaccacgt
     5101 tgaagtggtt tctgctcacc gcacccccga taaactgttc agcttcgccg aaagcgccga
     5161 agagaacggt tatcaggtga ttattgcggg cgcaggtggc gcagcgcacc tgccaggcat
     5221 gattgccgcc aaaacgctgg tgccggtgct gggcgtgcca gtacagagcg ccgcactgag
     5281 cggtgtcgat agcctctact ccatcgtaca aatgccgcgc ggcattccgg tgggtacgct
     5341 ggcgattggt aaagctggcg cggcaaacgc ggcattactg gcagcacaaa ttctcgcgac
     5401 tcacgataaa gagctacacc agcgtctaaa tgactggcgc aaagcccaga ccgacgaagt
     5461 gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct cggtaacggg
     5521 cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt ctggccggtc
     5581 gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac cgctgagatc
     5641 gaacgctggc cggaaaccgc attaacccgc gagctggcgc gtcatccggc ctttgtgaac
     5701 cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga agcagctttt cgataagctc
     5761 cacctgccga ccgcgccgtg gcagttactt gccgatcgca gcgagtggcc tgcggtgttt
     5821 gatcgtttag gtgaactggc gattgttaag cgtcgcactg gtggctatga cggtcgcggt
     5881 caatggcgtt tacgcgccaa tgaaaccgaa cagttaccgg cagagtgtta cggcgaatgt
     5941 attgtcgagc agggcattaa cttctctggt gaagtgtcgc tggttggcgc gcgcggcttt
     6001 gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg tattttgcgc
     6061 accagcgtcg cttttccgca ggccaacgca cagcagcagg cgcaagccga agagatgctg
     6121 tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg ttttgtcacc
     6181 ccgcaaggtc tgttgatcaa cgaactggca ccgcgtgtgc ataacagcgg tcactggaca
     6241 caaaacggtg ccagcatcag ccagtttgag ctgcatctgc gggcgattac cgatctgccg
     6301 ttaccgcaac cagtagtgaa taatccgtcg gtgatgatca atctgattgg tagcgatgtg
     6361 aattatgact ggctgaaatt gccgctggtg catctgcact ggtacgacaa agaagtccgt
     6421 ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg tctgactgcg
     6481 acgctggaag ccttaatccc gctgctgccg ccagagtatg ccagcggcgt gatttgggcg
     6541 cagagtaagt tcagttaagc aggtgctcat ttgattgccg gatgcggcgt aaacgcctta
     6601 tccggcttac cgctccggta cccattgtag gcctgataag acgcgtcaag cgtcgcatca
     6661 ggcatggtgc accgattgcc ggatgctctc atcactacag cgaaatacag gtccctgctt
     6721 cgcccgccag cgtatcttca atccgcgata acgccccaat ccacgcgggt ttaccacggc
     6781 ttctgacata accactcacc gccgttacct tcggccccat cgaaccatcg gctttggcaa
     6841 atggcgctaa ctcatccggt gtggcatggc gaatggcgcg ttgctgcggc gttccccagt
     6901 tttcatatac cgcatcagca tcggtgagga tcaccagccc atcggcgtta atctgctcgg
     6961 caagcaaggc agcggcgaga tctttatcaa tcaccgcttc actccctgcc ccgtcttccg
     7021 tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg agcaacaact
     7081 cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc cggcgcaaat
     7141 atttaccatc acgtttcatc tgccagccgt aagccgcttc cagtgcctct tgttcttctg
     7201 gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca tcaggcgaaa
     7261 cctcaatgcg cgtcagcacc gtcgtaacag gcggcatctg cggctgtgcg ctcaagctct
     7321 gcgccagcat atagccaatc atcccctggc tttccgccac cagcacatcc agcggatacg
     7381 gttctacctt tttccacgcc agattctgta acgccagcag ccccacctgc ggcccgttgc
     7441 cgtgaacaat cgccagccga taagaacggg ccaggcgtgc cagcgcgggt acagcactgg
     7501 cgatattgcg atattgattt tccgccgtca gcgcctcacc gcgctggagt aaggcgttgc
     7561 ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt ggccaagcca
     7621 gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg aatcaatcgc
     7681 aacagcggta cgtttcgggc aactcagagc atggacaaag tgcaggagtg gcgaggcgaa
     7741 atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa cgagttgaag
     7801 attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca tctaaagcgc cataatacca
     7861 ggccgccagc agcattccgc tcaacgtgtc atcatgactt ggcgttaatc cggggccttt
     7921 acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa aacagtgacg
     7981 aaactggcgt aattcagcgg gcagcggatc gcttgccgcc atcgccagtg gtccgaaaag
     8041 tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgaaggcgg
     8101 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     8161 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     8221 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     8281 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8341 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8401 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8461 acgccagcgg tgctcgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8521 cgataccggt attgatgagt ggcgtaatgc cggtttctac cacgcgacga atgtccagtc
     8581 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8641 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8701 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8761 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8821 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8881 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8941 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     9001 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     9061 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     9121 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     9181 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     9241 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9301 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9361 tgccgcccat tggcccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9421 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9481 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9541 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9601 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9661 atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagt tttttattgc
     9721 cgccggcgac tggcgaccat tggtaatgca caaccggttt gctggcgctt tgtagctcca
     9781 gcgcaaagct gcgtaatccg atgttaatca cggcgacgtt ttccagtaat gacggtgtgt
     9841 gttgctgagt ggcaggcgaa agcggacgaa tcagtgccgc cgccagcaag gtggcttccg
     9901 gcagcgaact cactaccgcg atccctgcat cttccagcgt ggcgatttgc tgcgagcggc
     9961 attgcgggtc acgttcagtg cctgtcaccg tggcaatggc atacagtggt tgattgtctg
    10021 aacgcgcggc acatgctttt tgccaggcgc ttaccagcga ggcggctgga tcggcggtcg
    10081 caccgaagcc aatcacgaca tcaagcagca atacgcgcac ttgcggttta gcgccgagat
    10141 cggcaattaa ctgattgcgt aaggctgggt cgatcatcgg atggggacgc ccgacggtgt
    10201 agaaatcatc gccgaggtca ataatctggt gaccatcggc gtcgagcatc atgccgtgat
    10261 gatgggtatc gtcggcttcc acgccaaggt gtccagcaag taatcccgcc gcttcggcag
    10321 ccagcgtacc gccggtatac aaaccgcaaa taaatccgct gctggcaggc gttatcgcgt
    10381 tacgtcgcgc cgtaacgcgt gaaagcaggc aagccaggcg tgcggcctca tccagcgagg
    10441 aggcaaacca gacattctcg tcgcgtgcca ccgccggggt ataacctaaa aacagcgcca
    10501 ccgtcggttt gccggttgct ttcatggcat taacaatttt cagacgcaca gcttcggcag
    10561 gtggttttga aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc atttccagcg
    10621 ctgttagcgc actgatgccg cccacttcac ggctgaggtc gcgcccgcca aggccaatcg
    10681 cgtgagtaat tccctcccct gccagcgcaa tctgcgaaca cagctcctga atcccggtac
    10741 cggaagcgcc aatgacgcca atattgcctt ctggcatcac gttagcaaaa gccagcggtg
    10801 tgccggcaat catcgacgta ccgcagtccg gccccatcac cagcaagcct ttttcccgcg
    10861 cgcgggtttt aagttggatt tcatcttcca gcgtgacgtt atcggagaac atcatcacgt
    10921 tgaggttgcg atccagcgcc tggtttgtca gctccgccgc atactcgcca gccactgaaa
    10981 tcagcgccag attggcatcg gataatttct gacaggcact gtcccagcga cgcacctgcg
    11041 tcaaggactg gctgctgcct gacccctgcg ccagttgttt tagcgcctct tcaagctgct
    11101 gcataatcgc ctgcgcgatc cccgcatccg ccgcttcgct acgtattgcc acgcaaatat
    11161 cgttcggcgt ggcgttatta aaatcgtcat gccagaaacc tgtggtatct aataacgctt
    11221 tattggcggg cgtacccatc attacggaaa catcatcaac attttctgat tcgctgagtt
    11281 ttcgtgaaat aatcattaaa ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt
    11341 ggatcataac aactccttaa aaattgcacc catggcaatt gcacgagcag tgttaatgtg
    11401 gcgattacgg tatcgatgac aacggataaa taaggtgatg aatatcacac agggattatt
    11461 aacaaaagaa ttcaaataac cgccatgcta ttacgtgtt
//
LOCUS       DAFAVF010000131        11411 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFAVF010000131 DAFAVF010000000
VERSION     DAFAVF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.131"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            254..757
                     /gene="dps"
                     /locus_tag="LGK42_003433"
                     /gene_synonym="pexB"
     CDS             254..757
                     /gene="dps"
                     /locus_tag="LGK42_003433"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBK2659649.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1161..1907
                     /gene="glnH"
                     /locus_tag="LGK42_003434"
     CDS             1161..1907
                     /gene="glnH"
                     /locus_tag="LGK42_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBK2659650.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2046..2705
                     /gene="glnP"
                     /locus_tag="LGK42_003435"
     CDS             2046..2705
                     /gene="glnP"
                     /locus_tag="LGK42_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBK2659651.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2702..3424
                     /gene="glnQ"
                     /locus_tag="LGK42_003436"
     CDS             2702..3424
                     /gene="glnQ"
                     /locus_tag="LGK42_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBK2659652.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3541..5763
                     /gene="ybiO"
                     /locus_tag="LGK42_003437"
     CDS             3541..5763
                     /gene="ybiO"
                     /locus_tag="LGK42_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBK2659653.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPPPGEPTL
                     "
     gene            complement(5760..6686)
                     /gene="rlmF"
                     /locus_tag="LGK42_003438"
     CDS             complement(5760..6686)
                     /gene="rlmF"
                     /locus_tag="LGK42_003438"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBK2659654.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6962..7222
                     /locus_tag="LGK42_003439"
     CDS             6962..7222
                     /locus_tag="LGK42_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2659655.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7486..9768
                     /locus_tag="LGK42_003440"
     CDS             7486..9768
                     /locus_tag="LGK42_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBK2659656.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVTTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9810..10487
                     /gene="ybiX"
                     /locus_tag="LGK42_003441"
     CDS             9810..10487
                     /gene="ybiX"
                     /locus_tag="LGK42_003441"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBK2659657.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10561..10827
                     /gene="ybiI"
                     /locus_tag="LGK42_003442"
     CDS             10561..10827
                     /gene="ybiI"
                     /locus_tag="LGK42_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBK2659658.1"
                     /translation="MASGWANDDAVNEQINSTIEDAISRARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLHKPAYTGYNRRGSKDSQLR"
     gene            11092..11352
                     /locus_tag="LGK42_003443"
     CDS             11092..11352
                     /locus_tag="LGK42_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBK2659659.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         2819 a         2953 c         2860 g         2779 t
ORIGIN      
        1 ctacttttcc tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa
       61 aattcctggc gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc
      121 tttattagtt ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat
      181 agcggaacac atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag
      241 aggatatgaa attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta
      301 tacccgcaac gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca
      361 ggttatccag tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg
      421 cgctaacttc attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca
      481 tctggatacc atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca
      541 agttatcaac agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga
      601 tcacctgaaa gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat
      661 tggcgaagcg aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga
      721 taaattcctg tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac
      781 tttagtaccc gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc
      841 aacgcactta tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat
      901 ccacatcatc acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata
      961 atcactgtgt tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct
     1021 tcagggtatt gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga
     1081 gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg
     1141 gatagaaaaa aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc
     1201 tggcttttgc ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg
     1261 ccttcgttcc gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt
     1321 gggctgccat cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg
     1381 ggatcattcc ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca
     1441 ccgacgagcg taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag
     1501 tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg
     1561 ttgctgtgaa gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag
     1621 atctgcgtca gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag
     1681 acgccgttct gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc
     1741 agttcaaagc ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag
     1801 gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa
     1861 cttacaacga aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct
     1921 gtaaaacgca ctggcagttc cctctcccct atggggagag gattagggtg aggggcgcaa
     1981 acccgctccg gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac
     2041 aacatatgca gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg
     2101 ccaaaatgac cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc
     2161 tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta
     2221 ttgaagtgat ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc
     2281 cgatggcgtt taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga
     2341 tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag
     2401 gttttcgtga agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca
     2461 ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca
     2521 ttaaagacac ctcgctgttt attgttatcg gcgtggcgga actgacccgt caggggcaag
     2581 aaattattgc cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc
     2641 tgattattac cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc
     2701 tgtgattgaa tttaaaaacg tctccaagca ttttggtcca acccaggtgc tgcacaatat
     2761 cgatttgaac attgctcagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa
     2821 atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt
     2881 cgatggcctg aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcggg
     2941 tatggtgttc cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt
     3001 tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct
     3061 gctggcgaaa gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg
     3121 tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt
     3181 tgatgaaccg acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca
     3241 ggatctggct gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga
     3301 gaaagtagct tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc
     3361 gcaggtattg atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc
     3421 ttaacttctc tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa
     3481 atcgcccctc ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc
     3541 atgcggtgga tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc
     3601 atacccggcg ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg
     3661 gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc
     3721 tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg
     3781 gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc
     3841 gaagtcagcc gccattatgg tgaagccctt tccgcccgtt tcggacaact ttatcgcaat
     3901 atcaccggct ccccgcataa gccgtttaat ccacaaacct tcagcaatgc gttgacccat
     3961 ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgtct gtgcgcactg
     4021 ccgctgtatc gcaaaatggg ccagtgggcg cggcaaaaaa atcgcgaacg cagcaactgg
     4081 ctgcagcttc cggcgatgat tatcggggcg tttattatcg acctactgtt actggcactg
     4141 acattgtttg tcggccaggt attaagcgac aacctgaatg cgggcagtcg caccatcgct
     4201 ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta
     4261 cgcctgattt tttgcccaaa cgtggctgaa ctgcgcccgt tcacgattca ggacgagagc
     4321 gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt
     4381 gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc
     4441 atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag
     4501 attacccagc atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc
     4561 cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc
     4621 ttttcgttgt tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc
     4681 ctggcgatta ttggtattgc agcgtttgtt tccggtatgt tttcccgctg gctggcaaaa
     4741 accatcactc tctcgccaca tactcagcgt aactatccag agttgcaaaa acggctgaat
     4801 ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg
     4861 ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg
     4921 cagaaaaccg tagatatcct gatccgtatc gcacttattc tttttttctc ggcggttggc
     4981 tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg
     5041 ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt
     5101 atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg
     5161 ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa
     5221 gatattatca ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggta
     5281 actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc
     5341 caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc
     5401 gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat
     5461 aaagccaatc aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc
     5521 gggctgatta ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt
     5581 acactgcgtg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc
     5641 gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat
     5701 caggtgctgc ctgctccggg cgcggccccg gctgaaccgc cgccggggga gccaacgctt
     5761 taacgttggc gattaacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata
     5821 aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac cgcgcccacg
     5881 tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag cgaggtaaac
     5941 cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaaggcgact
     6001 tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg
     6061 ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc ggcggaatcg
     6121 tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat gatgccgtta
     6181 aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta
     6241 gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa
     6301 cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc aacgccgata
     6361 tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg
     6421 tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc
     6481 cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc
     6541 ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa ttgcctgagt
     6601 tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga
     6661 tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg taagataccc
     6721 gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac cttaacatgg
     6781 ctatggttta tggcaattca tcataaaaat cagcattatg catgtcattg atttgtctta
     6841 ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt tgcgtcattt
     6901 tcatcgccca ggcgacctac acttatcagg cactacccgt tgtatttatg aggatgtcat
     6961 tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct ctctcacggc
     7021 ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg ccggtaccgt
     7081 ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg
     7141 cgagcaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg
     7201 tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt
     7261 ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc aatgtaaata aatgtatttc
     7321 tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta
     7381 tcacttgcac gaacactatc acgagattaa cagtggcatc gcatccgcag agaggctttc
     7441 tcgtggcagt gaaaatttca acatataaaa aaagtcacct gcaaaatgga aaacaatcgc
     7501 aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg tattggcatc
     7561 acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta acgcggatga cacgctggtt
     7621 gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc gaaattctcg
     7681 cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt gattaaagat
     7741 cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcgtggg tgcgtttttt
     7801 gcgggtgaga acggtaactc caccactggc gacgccattt atatgcgcgg tgctgatacc
     7861 tctaacagta tttatattga tggcattcgc gatatcggca gcgtctcgcg cgacaccttc
     7921 aatactgagc aggtcgaagt gattaaaggg ccttccggca ccgactacgg gcgcagcgcg
     7981 ccgacgggct cgatcaatat gatcagcaag cagccgcgca atgattccgg cattgacgcc
     8041 tccgccagta ttggcagcgc ctggttccgc cgcggcacgc tggacgtcaa tcaggtcatt
     8101 ggtgatacca ctgcggtgcg cctgaatgtg atgggcgaaa aaacgcacga tgccggacgc
     8161 gacaaagtca aaaatgagcg ttacggcgtc gccccttcta tcgcttttgg ccttggtaca
     8221 gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc acaacacgcc agacggcggc
     8281 attccgacca tcggtttgcc gggttattct gccccatctg cgggaacagc ggctctgaat
     8341 cattccggaa aagttgatac tcataacttt tacggcacgg attccgatta cgacgattcg
     8401 accaccgaca ccgccaccat gcgttttgag cacgacatca acgataacac caccattcgc
     8461 aatactaccc gttggtcgcg cgtaaagcag gattacctga tgacggcgat tatgggcggg
     8521 gcgtcgaata ttactcagcc caccagcgat gtgaatagct ggacctggtc acgcacggcg
     8581 aataccaaag atgtgagtaa taaaattctc accaaccaga ccaacctgac ctcgacgttc
     8641 tataccggtt ctatcggtca tgatgtcact accggcgtgg aatttacccg tgaaacgcag
     8701 actaactacg gcgttaatcc ggtgacgtta cccgcggtaa atatttatca tcctgacagc
     8761 agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg ccaatggtca gacggatacc
     8821 ttcgcaattt acgccttcga tacgctgcaa atcacccgtg attttgagct gaacggcggg
     8881 atccgtctgg ataattatca tactgaatat gacagtgcca ccgcctgcgg cggcagcgga
     8941 cgcggtgcca tcacctgccc aactggtgtg gcaaaaggtt ctccggtcac caccgtcgac
     9001 accgccaagt cgggcaatct ggtgaactgg aaagccgggg cgctgtatca cctgacggaa
     9061 aacggtaatg tctatattaa ctatgccgtt tcccagcagc ctccgggcgg caacaacttc
     9121 gcccttgcgc agtctggcag cggtaacagt gccaaccgca ccgattttaa accgcaaaaa
     9181 gccaacacca gcgagattgg caccaaatgg caggttctgg ataaacgcct gttgctcacc
     9241 gccgcgctat tccgtactga tatcgaaaat gaagttgagc aaaatgatga cggaacttac
     9301 tcgcaatacg gtaagaaacg tgtcgaaggc tatgagatat ccgtggccgg gaatatcact
     9361 cccgcgtggc aggtgattgg cggctatacc cagcaaaaag caaccatcaa aaacggcaaa
     9421 gatgttgccc aggatggttc ctcatcgctg ccgtataccc cggagcacgc cttcacctta
     9481 tggagccagt atcaggcaac cgatgatatc tctgttggcg cgggcgcacg ctatatcggc
     9541 agtatgcata aaggttcaga cggcgcggtg ggaacgccag cgtttaccga aggttactgg
     9601 gtcgccgatg ccaaactggg gtatcgggtt aatcgcaatc tcgacttcca gctaaacgtt
     9661 tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca agagcggcta ccgttatcac
     9721 ccgggcgagc caagaacctt cttgctcaca gccaatatgc atttctgatt cagatgtggg
     9781 gcgcaggccc cactttttgg agaacttgta tgatgtacca cattcccggc gtgttatcgc
     9841 cacaggacgt cgcccggttt cgcgaacaac tggaacaagc cgaatgggtg gatggacgcg
     9901 tcaccaccgg cgcacaaggt gcgcaagtta agaacaatca acaggtcgac acccgcagca
     9961 cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt taaccaacat gctttattct
    10021 ttgccgcggc cttgccgcgt accctttcca cgccgctgtt taatcgctat cagaacaatg
    10081 aaacctatgg tttccatgtg gatggcgcag tacgcagcca tccacaaaac ggctggatgc
    10141 gtactgacct ttctgccacg ctgtttttaa gcgatccaca aagctacgac ggcggcgaac
    10201 tggtcgttaa tgacaccttc ggacaacatc gggtaaaact cccggcaggc gatctcgtgt
    10261 tgtatccctc cagcagcctg cattgcgtga cacccgtaac ccgcggcgta cgagtggcat
    10321 catttatgtg gatccagtcg atgatccgcg atgataaaaa gcgcgccatg ctgtttgaac
    10381 tggacaacaa tattcagtcg ctgaaaagcc gctacggcga aagtgaagag atcctgtcgc
    10441 tgcttaatct ttatcataat ctgctgcggg aatggtcgga gatctgatgc ttgaactgcc
    10501 cgagttcgtc cctggattag ctacacttaa ctgtacaagt attgatatgg ggaggtcgat
    10561 atggcatccg gttgggctaa cgatgacgcc gtcaacgaac agatcaacag tacaattgaa
    10621 gatgcgattt cccgcgctcg gggtgaaatt ccgcgcggcg aaagcctgga tgaatgtgaa
    10681 gagtgcggtg cccccatccc gcaggcccgt cgggaagcca ttcctggcgt gcgcttatgt
    10741 attcattgtc agcaggagaa agatttacat aaaccagctt atacaggata taatcgcaga
    10801 ggttcgaaag acagccagtt acgttaacta caactggcga gatgcatagc gagtataatt
    10861 tgtattttgc gtggcattcc gtgcctttaa cgctgctgtg actgccacgc ggtataaaac
    10921 aagttcataa gtacaacaaa taaatggttt atcagtaggt tagatatcaa tcaatttatt
    10981 tgaacaaggc ggtcaattct cttcgatttt atctctcgta aaaaaacgtg atactcatca
    11041 catcgacgaa acaacgtcac ttatacaaaa atcacctgcg agagattaat tatgaaaact
    11101 atcaatactg ttgttgctgc tatggctctt tcaactctgt catttggcgt gtttgccgcg
    11161 gaaccggtaa cggcatccca ggcacagaac atgaataaaa tcggcgtggt ttctgccgat
    11221 ggcgcatcca ccctcgatgc cctggaagcg aaactggctg agaaagccgc ggcagccggt
    11281 gccagtggat acagcatcac ttccgccacc aacaacaata aattaagcgg tactgcggta
    11341 atttataagt aattcgtagg acggataagg cgtttacgcc gcatccggca acagatgcct
    11401 gatgcgacgc t
//
LOCUS       DAFAVF010000132        11355 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFAVF010000132 DAFAVF010000000
VERSION     DAFAVF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.132"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(106..2496)
                     /gene="gcd"
                     /locus_tag="LGK42_003444"
     CDS             complement(106..2496)
                     /gene="gcd"
                     /locus_tag="LGK42_003444"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBK2659660.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            2702..3238
                     /gene="hpt"
                     /locus_tag="LGK42_003445"
     CDS             2702..3238
                     /gene="hpt"
                     /locus_tag="LGK42_003445"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK2659661.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(3279..3941)
                     /gene="can"
                     /locus_tag="LGK42_003446"
     CDS             complement(3279..3941)
                     /gene="can"
                     /locus_tag="LGK42_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBK2659662.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            4050..4976
                     /locus_tag="LGK42_003447"
     CDS             4050..4976
                     /locus_tag="LGK42_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2659663.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            4973..5743
                     /locus_tag="LGK42_003448"
     CDS             4973..5743
                     /locus_tag="LGK42_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2659664.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            5848..6288
                     /locus_tag="LGK42_003449"
     CDS             5848..6288
                     /locus_tag="LGK42_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK2659665.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            6352..7581
                     /locus_tag="LGK42_003450"
     CDS             6352..7581
                     /locus_tag="LGK42_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK2659666.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVLDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(7585..7965)
                     /gene="panD"
                     /locus_tag="LGK42_003451"
     CDS             complement(7585..7965)
                     /gene="panD"
                     /locus_tag="LGK42_003451"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBK2659667.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            8239..9141
                     /gene="rpnC"
                     /locus_tag="LGK42_003452"
     CDS             8239..9141
                     /gene="rpnC"
                     /locus_tag="LGK42_003452"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBK2659668.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            complement(9215..10066)
                     /gene="panC"
                     /locus_tag="LGK42_003453"
     CDS             complement(9215..10066)
                     /gene="panC"
                     /locus_tag="LGK42_003453"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBK2659669.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(10078..10872)
                     /gene="panB"
                     /locus_tag="LGK42_003454"
     CDS             complement(10078..10872)
                     /gene="panB"
                     /locus_tag="LGK42_003454"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBK2659670.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(10985..>11355)
                     /locus_tag="LGK42_003455"
     CDS             complement(10985..>11355)
                     /locus_tag="LGK42_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2659671.1"
                     /translation="QNCVRVTNIETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIE
                     GLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDG
                     TIPIEAGEFKATSTFQVTYP"
BASE COUNT         2698 a         2788 c         3000 g         2869 t
ORIGIN      
        1 tagctgtgca atccattgat tttgcacaat tttgtaggct ggataaggcg ttcacgccgc
       61 atccggcatg aacaacgcgc acgttgtcag caatctgagc aagtcttact tcacatcatc
      121 cggcagcgca taagccacaa tatagtcgcc catcttcgta ccaaatgaac cgtgaccgcc
      181 tgcggagatc accacatact gcttaccatt cacttcatag gtcattggcg tagcctgacc
      241 acccgctggt aaacgaccct gccacagttt ttcaccgttg ctcatgttgt aagcgcgcag
      301 gtagttatct gccgtagcgg cgataaacag cacgttaccc gccgtggaga ttggcccgcc
      361 cagcatcggc atacccatat tgaacggcac cggtacaggc atcgggaacg gcatactgtc
      421 ctgcggcgta ccaatacgtt tcttccacac cacttcattg gttttcagat ccagcgccga
      481 gatataaccc catgctggct gtttacatgg cagaccaaac ggtgagagga acgggttgag
      541 cgtgacacca tacggtacac cgtactgtgg ctgaatgccg gattccgtac ccgtgccttt
      601 ggcatctttc ggctgctcca tcgggttacc cggaccgcgt gggatcagtt tcgaaacaaa
      661 cggcagtgcc attgggttgg caattgccac ttcacgattt ggatcaacgg aaatcccgcc
      721 ccattcaaac attcccaggt tacccgggaa gaccagcgtg ccctgttcag atggcggcgt
      781 gaaaatgcct tcatagcgca actggtggaa catcacgcgg cacaccagtt ggtcaaacat
      841 ggtggctccc cacatatccg caccgctcaa atctttcttc ggacggaagc tcagttcaga
      901 gaacggctgg gtcggagtaa cgtaatcgcc tttcgcagca ccttgcggca ccggtttttc
      961 cggggccgga accaccagtt cgccattgcg acgatcgagc acaaaaatgt tgccggtttt
     1021 cgccggagcg taaataactg gcactttctg accattaacg gtgatgtccg ccagcgtcgg
     1081 ctgtgccgga agatccatgt cccacaggtc gtggtgaacg gtctggtagc tccacgccag
     1141 tttcccggta gtggcattca gcgccagaat cgagctggca taacgttcct gttccggtgt
     1201 gcggttaccg ccccagatat ccggcgtggt cacgcccatc ggcagataga ccagatccag
     1261 cttcgcgtca taggccgctg gtgcccagga gtttggcgag ttaaaggtaa aggtgtgttc
     1321 gtcagacggg attgcgttcg gatctttcgc gccgggatca aaagcccaca gcagctcccc
     1381 ggtgttgaca tcaaaaccac ggatcacgcc agacgtttcg cgggttgaga agttatcggt
     1441 aactgaaccg gccatcacga tggttttatc ggtgataatc ggtggcgaag tcggttcata
     1501 cagacccggt ttggtgtctg gcatattgct ttgcagattg agcacgcctt tattggcgaa
     1561 ggtttcgcac agtttgccgt tttcagcgtt aatcgcaatc agtcgaccat cattgaccgg
     1621 aagaatgata cgacgcgggc aatccgccat cacttccggc gaagccgttt ctgctttggc
     1681 ttcatgataa gagacgccac ggcaggttac gtgctggaaa gactcgttag ttttcagctc
     1741 aggatcgtaa tgccatttct ctttgccgct ggcggcatcg agcgcaaaca ggcgctggtg
     1801 agcggtacac aggtaaaggg tgtcgcccac tttaatcggc gtcacttcat tggtgatttc
     1861 gcccggatcg ttcggctgct tcacatcgcc agtacggaac acccaggctt ctttcagatt
     1921 atggacgtta tcggcgttaa tttgtttcag aggtgaaaag cgttgacctt cctgattgcg
     1981 accataggca ggccagtcct gatcggcaac gggtgagata gcttccgcag gtgtggcatt
     2041 ggcgcttaag gtgccgttga tttcctgcgg atcgttaaat ccggcccagg tcaggatacc
     2101 accgctaatc agcagtgcca ccaccagtgc ggcaactgcg ccgctggcag gaatgaccag
     2161 gcgacgccag acaaacggca ggatcagcca gatgccgaag aagaccagaa tgtcgctgcg
     2221 cggagtcagc gcccagaagt cgaaaccaac ttcccagacg ccccaaatca tggtgccgag
     2281 cagcagggct gcgtacagcc aaagcgcggc acgtttactg cgccacagca tccaggcgac
     2341 gccgagcatc acaagaccag cgatagggta gtaccaggag ccgccaatcg cgaccagcca
     2401 gcctccgcca atgagtagat acagcccgca aagcgctgca aaaagggctg ttagcgtgac
     2461 gagtaatcgt cgcgagcctg tattgttaat tgccataaag agacaccatt tcaattcatt
     2521 aatattttag tagcaattaa ttataggttt taacatgtgt gatcgtcatc acaattcgag
     2581 ctttattaac agattccgcg aatgaatagt tttactggta tactgcgtgt cttgcgcttt
     2641 gttgcggtgc caaaacctgc ccgtgcgaag tgatttgttt ttaaatcata tggttagaga
     2701 tatgaaacat actgtagaag taatgatccc cgaagcggag attaaagcgc gtatcgccga
     2761 actgggtcgt cagattactg agcgttacaa agacagcggc agcgatatgg tgctggttgg
     2821 tctgctgcgt ggctcattta tgtttatggc ggacctgtgc cgtgaagttc aggtatctca
     2881 tgaagtcgac tttatgaccg cctccagcta cggtagcggc atgtccacca cccgtgatgt
     2941 gaaaatcctc aaagatctgg atgaagatat ccgtggcaag gacgtgctga ttgttgaaga
     3001 tatcatcgac tcggggaata cactgtcgaa agtgcgtgag atcttaagcc tgcgcgaacc
     3061 gaagtcgctg gcgatttgta cgctgctgga taaaccgtcc cgtcgtgaag tgaacgtccc
     3121 ggtagaattt atcggtttct cgatcccgga tgagtttgtg gtgggttacg gcattgatta
     3181 cgcacagcgt taccgtcatc tgccgtatat cggcaaagtg attctgctgg acgagtaagt
     3241 gtgaagttgc cggatgtgtt gcatccggca tggcattttt atttgtggtt ggcgtgtttc
     3301 agcttgaggt tggaaatccc gtgacggtaa cgttgctcaa gggtttcgcg gttggtcgct
     3361 gtgacatcca gatcacgcag caagccgtcg tgaatgccgt aggcccagcc gtgaatggta
     3421 actttctgcc cgcgtttcca cgctgattgc ataatggtgg agtggcccag gttatacacc
     3481 tgttccatga cgttcagttc acacaaggta tccagacggc gctcttgcgg catttcgccg
     3541 agcaatgagc tatgtttgaa ccagatatcg cggatatgca gcagccagtt gttgataagc
     3601 cccagttccg ggttttcaac tgcggcttgt acgccgccgc aaccgtagtg gccacagata
     3661 ataatgtgtt caacttcgag tacatccact gcatactgaa ccacggaaag gcagttcagg
     3721 tcagtgtgaa tgaccaggtt agcaacatta cggtgaacaa agagttcgcc cggctcaaga
     3781 ccggttaaac gttctgcagg aacgcgactg tcggaacatc caatccatag aaagcgcggt
     3841 ttttgcgctt gtgccagttt ctcaaaaaac ccgggatcct cttccaccag catttttgac
     3901 catagtgcat tgttgctgat gagtgtatct atgtctttca tggaggttaa cgacctgtaa
     3961 ccaaataatt acgtttggct aatatagggc aactccggga cgatttaaac cacagataaa
     4021 gtgtaagaac gtaaggtaag taaaaattta tgaccattgc actggaactt caacagctta
     4081 aaaaaaccta tccaggcggc gttcaggcgc ttcgtgggat agatttgcag gtcgaagcgg
     4141 gtgattttta tgcgcttctc gggccgaacg gggccgggaa atcgaccact atcggtatta
     4201 tcagctctct ggtaaataaa acctccgggc gggtcagcgt atttggttac gatctcgaga
     4261 aggatgtcgt gaacgctaaa cgtcagttgg gactggtgcc gcaggaattt aacttcaacc
     4321 cgtttgaaac cgtgcagcaa attgtggtga atcaggcagg gtactatggc gtggagcgca
     4381 aagaagcgta catccgcagc gaaaagtatc ttaaacaact cgatctatgg ggaaaacgca
     4441 acgaacgtgc gcgtatgtta tctggcggga tgaagcgccg tttaatgatt gcccgtgcgt
     4501 taatgcatga acctaaacta ctgattctcg acgaaccgac cgcaggcgtg gatattgaac
     4561 ttcgccgctc aatgtggggc tttttgaagg atttaaacga caaaggcacc accatcattc
     4621 tcaccacaca ctacctggaa gaagcagaaa tgctgtgccg caatatcggc attattcaac
     4681 acggtgagct ggtggaaaat acctcgatga aggcgctgct ggcgaagctg aaatcggaaa
     4741 cctttattct cgatctcgca ccgaaaagtc cgttaccgaa gctcgatggc tatcagtatc
     4801 gactggtcga taccgcgacg ctggaagttg aagtgctgcg tgagcagggg atcaacagcg
     4861 tatttacgca gttaagtgag cagggcattc aggtattaag tatgcgtaac aaagctaacc
     4921 gtctggaaga gctgtttgtt tcactggtta atgaaaaaca aggagatcgc gcatgatgca
     4981 tctttactgg gtggcgctaa aaagcatctg ggcgaaagag atccatcgct ttatgcgtat
     5041 ctgggtgcag acgctggtgc cgccagtcat caccatgacc ctttacttta ttatcttcgg
     5101 taacctgatt ggttcgcgta ttggcgatat gcatggcttc agctatatgc agttcatcgt
     5161 accggggctg atcatgatgt cggtgatcac caatgcctac gccaacgttg cgtcatcatt
     5221 ttttggtgcc aagttccagc gtaatattga agagctgctg gtagcgccgg ttccgactca
     5281 cgtcattatt gccggatatg tcggcggtgg cgtggcgcgt ggtctgtttg ttggcattct
     5341 ggtgacggca atttcactgt tttttgtgcc gtttcaggtg cattcgtggg tattcgttgc
     5401 cttaaccctg gtgctcacgg cggtgttgtt ctcccttgcg ggtttgctga acggtgtgtt
     5461 tgccaaaacg ttcgatgaca tcagcctggt gccaaccttt gtgttaacgc cactcacgta
     5521 tttgggtggg gtcttttact cactgacttt gttgccgccg ttctggcaag ggctgtcgca
     5581 cctgaaccca atcgtttata tgatcagtgg tttccgctac ggcttcctcg gtatcaatga
     5641 tgttccgctg gtcactactt ttggcgtact ggtggtcttt attgtggcgt tttatttgat
     5701 ctgttggtcg ctgatccaac gtggacgtgg tttgcgtagc taaggctatt tcctctcctc
     5761 tggatttggg ggagaggagt tttgacggct atcacccttt atcaacaatg gtcagggtag
     5821 actgattttc ggctaaggag gaaggcgatg ttaggttggg taattacctg tcacgatgac
     5881 cgggcgcaag agatactgga tgcgctggag aaaaaacatg gggcgcttct tcagtgccgg
     5941 gccgtgaatt tctggcgtgg gttaagctct aatatgctca gccgcatgat gtgcgatgct
     6001 ctgcatgaag cggactctgg tgagggtgtc atcttcttaa ccgatatagc cggagcgcca
     6061 ccgtaccgcg tggcttcatt attaagccac aaacactccc gttgcgaagt gatttctggt
     6121 gtcacgttac cgttaattga acagatgatg gcttgccgtg aaaccatgac cagttcagag
     6181 tttcgcgagc gtattgtcga actgggtggg ccggaggtga gtagtctttg gcaccaacaa
     6241 caaaaaaatc cgcctttcgt cctcaaacat aatttgtatg agtattaacc cacgattctt
     6301 atagcgcttt tgctacaata aaagcgttgt ttcaccctcg gttatttttt catgtacaaa
     6361 caagctgtta ttctcctgct gatgctgttt accgcaagtg tcagtgccgc gttacctgcc
     6421 cgttatatgc aaaccatcga aaatgctgcg atctgggcgc aaattggcga caagatggtg
     6481 accgtgggga atattcgggc cgggcaaatc attgccgtgg agcccactgc cgcaagttat
     6541 tacgcattta attttggctt tggtaaaggg tttatcgata aagggcatct cgagccagtt
     6601 caggggcgac aaaaagttga agacggtttg ggtgacctca acaagccgct gagtaatcag
     6661 aacttaatta cctggaaaga tacgccggtc tataacgcgc cgagtgcggg cagtgcgcca
     6721 tttggggtac tggcggacaa tttgcgctac ccgattttgc ataaactgaa agacaggtta
     6781 aatcaaacct ggtatcagat ccgtattggc gatcgactcg cctatatcag cgcgctggat
     6841 gcccaacccg ataatggcct gccggtgcta acctatcacc atattctgcg cgatgaagaa
     6901 aacacccgtt ttcgccatac ttcgacgacc acctcggtac gcgctttcaa taaccagatg
     6961 gcctggctgc gcgacagggg atacgcgaca ctgagcatgg cgcagctgga aggttacgtg
     7021 aagaataaga tcaatctccc tgcgcgagcg gtggtgatta cctttgatga tggcctcaag
     7081 tcggtgagcc gctatgcgta tcctgtattg aaacaatatg gcatgaaggc gacggcgttt
     7141 gttgttacct cgcgcatcaa acgtcacccg cagaagtgga acccaaaatc gctgcaattt
     7201 atgagcgttt ctgagcttaa cgaaattcgc gatgttcttg attttcagtc acatacccat
     7261 tttttgcatc gggtggatgg ctatcgccga cccatattac tgagccgtag tgagcacaat
     7321 attctgtttg attttgcacg ttcacgccgc gctctggcgc aatttaatcc gcatgtctgg
     7381 tatctttcgt atccgtttgg cggatttaat gacaaagccg tgaaggcagc aaacgatgcc
     7441 ggatttcacc tggcggtgac aaccatgaaa ggcaaagtaa aaccggggga taatccgttg
     7501 ttactaaaac gactttatat cttaagaacg gattcgctgg agacgatgtc gcggctggtg
     7561 agtaaccagc cgcagggata acaatcaagc aacctgtacc ggaatcgctt tcgcggtacg
     7621 tttcatttca ttgtcgcctt caaaataggc gacattgggt cgccaggtgc gagcttcttc
     7681 atctggcatg gtaacgaagc tggcgatgat gacaatatcg ccgacgctgg cgcagtgggc
     7741 cgccgcaccg ttaacagaaa taattctcga accgcgttct gccgcgatgg cataagtgga
     7801 gaaacgcttg ccgttggtga cattccagat atcgatggct tcgttttcga gaataccggc
     7861 tgcgtcaaga aaatcctggt caatggcgca agaaccttca tagtgcaggt ccgcatgagt
     7921 cactttcacg cggtggagtt tgccctgcag catcgtgcga atcataactt ctacctttct
     7981 accctgtcgt taacgaagca ggcggtgcct gctttgagga aattctcacg cagtattgcc
     8041 cgatttttga tgagtgtcta ctcatctgat ggcatttgcg tcagcagttt gcgtaccgcg
     8101 ccgaagcgtg ccatgtatcg cctgtaacga attgaaattt ctctgaacgc tgctcggtaa
     8161 acactaaaga ggcggctgac gacggcgcgc cttgactgga tactgccttc acgcaagaca
     8221 acacggaaga cagggaagat ggatgcaccg agtaccacac cgcatgacgc ggtatttaaa
     8281 caatttttaa tgcatgcgga gacggctcgc gactttctgg agatacattt gccagtggaa
     8341 ttacgagaac tttgtgacct caacacgctt catttagagt cggggagttt cattgaagag
     8401 agcctgaaag gacacagcac ggacgtgctc tattccgtgc aaatgcaggg caatcccggt
     8461 tatctgcatg ttgtgattga acaccaaagc aagccggata agaaaatggc ctttcgcatg
     8521 atgcgttatt ctattgctgc catgcaccgg catctggagg cggatcacga taagctgccg
     8581 ctggtggtgc cgatactgtt ttatcagggc gaggccacac cttatccgct ctcaatgtgc
     8641 tggtttgata tattttactc gccggagctg gcacgacgcg tctataacag tcctttcccg
     8701 ctggtggata tcaccatcac accggatgac gaaatcatgc aacatcggcg gattgcgatt
     8761 ctcgaactac tgcaaaaaca tattcgccag cgcgacttaa tgttattgct ggagcaactg
     8821 gtcacgctga ttgacgaagg gtacactagc ggaagtcagt tagttgccat gcaaaactat
     8881 atgctgcaac gcggtcatac tgaacaagcg gatttgtttt atggtgtgct gagagacagg
     8941 gaaacgggag gggagtctat gatgacgctg gcgcagtggt ttgaagagaa agggattgag
     9001 aaggggattc agcagggaag acaggaggaa aggcaagaat tcgccctgcg tcttctaagt
     9061 aaaggaatgt ctcgggaaga cgttgcagag atgacaaatt tatctcttgc tgagattgat
     9121 aaggtaatta accttattta agttacctgt gttatgacag atgacgtggg gtaaattaat
     9181 aactggcgcc atcagccgta gcgccagtta agtattacgc cagctcgacc attttgttgt
     9241 cgatcaggcg agcatcgcca agccaggcgg ctaccagaat tactgcccgt ttgctggttt
     9301 cagaaacttc cagcaatgtg tcggcatcgc gaatctgaat atcatcggcg cggaagcctt
     9361 tttcattcag ttcttgcccc gcaatggtaa taatttcatc gagatcccgt tccccagcct
     9421 gcaatttgtc agcaatcgaa cttaaaactt tgtacagacc aggcgcaatt ttgcgttgtt
     9481 ccgccgtcag ataaccgtta cgggaactta gcgccagacc gtctttggcg cgcataattg
     9541 gcacaccgac aatctcaata tcgaagccca tatcggcaac cattttgcgg atcagcgcca
     9601 gttgctgaaa gtccttttca ccgaagcagg cgatgtccgg ctggaccagg ttaaacagct
     9661 tgctgacgat agtcgaaacg ccgcgaaaat gccccggacg gctcgcgcct tccagcatgg
     9721 tcgaaagccc gggaacgtca acgtaagtat gggtttcagt accgttcggg tagatctctt
     9781 ttaccgaagg ggcgaaaact aaatccactt tacgtttgtt tagcttctcg cagtcctcct
     9841 gcaaggtccg tggataacgt gccagatctt ccgggcggtc gaactgcatc gggttaacga
     9901 aaatactgac gacgaccaca tcggcgcggg ctttggcttc gtcgaccagc ttcatatggc
     9961 catcgtgcag attacccata gtaggcacca gcgccacgcg cttgccttcc atacgcaggc
    10021 ggcgaatttg ctgacgcagc agcggcaggg tttcgataat taacacaacg tgactcctta
    10081 atggaaactg tgttcttcgc ccggataaac gccggactcc acttcagcca tatactgccg
    10141 cacagccgcg cggatgtcgc ccgtttcggc gaggaaattt ttagcgaatt taggaatgtg
    10201 accgccggta atgccgaagg cgtcgtgcat cacgaggatc tgcccgtcag tgacgttgcc
    10261 tgcgccaatg ccaataaccg ggatcgccag tgcttcggta atacgttttg ccagttcaac
    10321 cggcacgcat tccagcacca gcagctgtgc cccagcggct tctaaggcta atgcatcgct
    10381 gagcagttga tcgcccgctt catcgccgcg cccctgaact ttgtagccac cgaaaatatt
    10441 cactgactgt ggggttaaac ctaagtgacc acatacagga acggcacgtt cggtcagcat
    10501 ttttacggtt tcgaccagcc actcaccgcc ctcaattttg accatattgg caccggcacg
    10561 cataaccgtt gcggcgtttt caaaggcttg ttccggcgtg gcatacgcca taaacggcag
    10621 gtcagccaac agcaggcagt ttggcgcgcc gcgacgtacg gcggcagtgt ggtaggcgat
    10681 atcctcgacg gtaacgggaa gggtggagtc gtggccctga accgtcatgc ccagcgaatc
    10741 gcctaccagc atgacgttaa gtccttcatc agcaaatagt ttcgcgaagc tgtagtcgta
    10801 agcggtgatg gtcgcgaaac gctttttatc ctgcttacat ttctgaagtg aagcaatggt
    10861 ggtcggtttc ataacgtgtc ctgataaatg atgtcgtgtt gcctggcatt ttatcagtca
    10921 cattggtggg ggcaatgatt tatcaattac cgcaccgcca gatgatggcg gtgcggtgga
    10981 cttattaagg gtaggttacc tggaaagtac tggtggcttt aaactcacca gcttctattg
    11041 gtatagttcc atcctgttgt agcgtggcct ggaaatggag agggtatgtt atacctgtat
    11101 cttgatccgg gtaaactcct cctgttgtat cgtctttgcc tctcgggtcg taatctttat
    11161 aaacagaatt tgaatcatta ggtttcaatg tcattagagg atttttacta gttgctaaac
    11221 cttctatgag tacaccgact ccttttgctg cgtcattacc tgtgagagta ttaccaagga
    11281 gttgcccgtt ttcagtacca acctttgttg aaactaattt tgtctcaata ttagtcacac
    11341 gaacgcagtt ttgaa
//
LOCUS       DAFAVF010000133        11262 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFAVF010000133 DAFAVF010000000
VERSION     DAFAVF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.133"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(37..273)
                     /locus_tag="LGK42_003456"
     CDS             complement(37..273)
                     /locus_tag="LGK42_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBK2659672.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="LGK42_003457"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="LGK42_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBK2659673.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="LGK42_003458"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="LGK42_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBK2659674.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..2600)
                     /locus_tag="LGK42_003459"
     CDS             complement(1875..2600)
                     /locus_tag="LGK42_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK2659675.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /locus_tag="LGK42_003460"
     CDS             3219..3989
                     /locus_tag="LGK42_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBK2659676.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /locus_tag="LGK42_003461"
     CDS             3980..4453
                     /locus_tag="LGK42_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBK2659677.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="LGK42_003462"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="LGK42_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBK2659678.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="LGK42_003463"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="LGK42_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBK2659679.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="LGK42_003464"
     CDS             5888..6382
                     /locus_tag="LGK42_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBK2659680.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="LGK42_003465"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="LGK42_003465"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBK2659681.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRDDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="LGK42_003466"
     CDS             complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="LGK42_003466"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBK2659682.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11141
                     /locus_tag="LGK42_003467"
     CDS             10227..11141
                     /locus_tag="LGK42_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2659683.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVLVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2921 a         2875 c         2643 g         2823 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccgacgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggt gataaatccg catacatcag ccctgcaatc agcaatcctg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatgtta tctggcgaac gcatctccag ccacaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccgaaca tcgtgaacat ttgctggagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gccgtgccgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaagg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatcttac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg cttactaccg
     1081 gtgaagataa tccactctgg cgtaccgtgg tgctacgcga cggcctgctg gtgcgccgga
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcagat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2041 gcgctgatag ttttattact tttcttaagc aacgaagcta tctggttgat gccccatcct
     2101 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2161 aaatcatgag tttcattcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2221 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2281 gtcgccgtag ggcacatgag caattctact gcctgggaat accaggagcg tgacaccatg
     2341 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2401 cgaggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg acgtaattgt
     2461 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2521 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2581 ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta
     2641 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2701 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
     2761 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta
     2821 cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt
     2881 aaatgatatc ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc
     2941 tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt
     3001 aaaggccaaa caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg
     3061 agagataatt cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat
     3121 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat
     3421 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgtg
     3601 cctgtttctg taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacggccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacaaatca
     3721 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caacttccca
     3781 ctttcagcaa tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat
     3841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc
     4081 gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac
     4141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     4261 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcactctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccagcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac cttaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtgacgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcact atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaagcg cgtagaaccg ctgccggaaa
     8221 gtgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctac aagatggcac aactttgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgtccag catgttgata
     8401 aaacaatgtg gaagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     8461 aaagcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg atttcccgtc
     8581 cggagtcgtg gggccgctgg tccaacgctc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga aggataattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctgcggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc ggcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggctg
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgctgaccat ttttaagatc agcaatattt ttacccagcc caacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gtactacagt gcgtcatatt ctcagccact
    10381 tacgcgaatg cggcgtctta acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accatgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgatg ataaaccagc gccagttacg cccaggggaa acgttttctg
    10561 aactgcaact ggctcgggcc gcgggtgtca gcccggttct agtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcacagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct tgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
    11221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg ta
//
LOCUS       DAFAVF010000134        11117 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFAVF010000134 DAFAVF010000000
VERSION     DAFAVF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.134"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(41..394)
                     /locus_tag="LGK42_003468"
     CDS             complement(41..394)
                     /locus_tag="LGK42_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBK2659684.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(532..2178)
                     /gene="groL"
                     /locus_tag="LGK42_003469"
     CDS             complement(532..2178)
                     /gene="groL"
                     /locus_tag="LGK42_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK2659685.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2222..2515)
                     /locus_tag="LGK42_003470"
     CDS             complement(2222..2515)
                     /locus_tag="LGK42_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK2659686.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2791..4047
                     /gene="yjeH"
                     /locus_tag="LGK42_003471"
     CDS             2791..4047
                     /gene="yjeH"
                     /locus_tag="LGK42_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBK2659687.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4063..4539)
                     /gene="fxsA"
                     /locus_tag="LGK42_003472"
     CDS             complement(4063..4539)
                     /gene="fxsA"
                     /locus_tag="LGK42_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBK2659688.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4876..6312
                     /gene="aspA"
                     /locus_tag="LGK42_003473"
     CDS             4876..6312
                     /gene="aspA"
                     /locus_tag="LGK42_003473"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBK2659689.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6430..7731
                     /gene="dcuA"
                     /locus_tag="LGK42_003474"
     CDS             6430..7731
                     /gene="dcuA"
                     /locus_tag="LGK42_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBK2659690.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7847..8185
                     /gene="cutA"
                     /locus_tag="LGK42_003475"
     CDS             7847..8185
                     /gene="cutA"
                     /locus_tag="LGK42_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBK2659691.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8161..9858
                     /gene="dsbD"
                     /locus_tag="LGK42_003476"
     CDS             8161..9858
                     /gene="dsbD"
                     /locus_tag="LGK42_003476"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBK2659692.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9895..10470
                     /locus_tag="LGK42_003477"
     CDS             9895..10470
                     /locus_tag="LGK42_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2659693.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10577..10652
                     /locus_tag="LGK42_003478"
     tRNA            10577..10652
                     /locus_tag="LGK42_003478"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10610..10612,aa:Phe,seq:gaa)
BASE COUNT         2618 a         2907 c         2753 g         2839 t
ORIGIN      
        1 tcaggcaata atacgtttgt cacccagcct ctggatcaca tcagttcggg cacttataaa
       61 cctgaccgat aatctggcta tccgtcggga caaaactgga caacattccc tgcgacgggc
      121 tactgatgcc ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc agatcgtttg
      181 ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc ggaaagccag ttgctttgct
      241 tacctgtcgc agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc acaatgcgta
      301 cactctgacc ggcagcactc aattcgttgc tggagctaca acccgccatc agtagcgcgg
      361 caccgacaat ccctgctaag tattttacgt gcatgttatt ccccataatc aatgagctgg
      421 acgccactcg cgtcgtccgt gtctgaatct tatactagaa agatgaccaa aagaaaaacc
      481 cccagacatt tctgcccggg ggtttgttta tttctgcgag gtgcagggca attacatcat
      541 gccgcccatg ccacccatgc cgcccatacc gccagcagcg cctaagtcag ctgcatcatt
      601 tttcggcagg tcggtaacca tgcactcggt ggtgatcatc aggccagcca cagaagccgc
      661 gtactgcaga gcagaacggg ttactttggt tgggtccagg atacccatgt cgatcatgtt
      721 gccgtattct tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt taacggtgtt
      781 agcaacaaca gacggttctt cgccgcagtt caggacgatc tgacgcagcg gagcttccat
      841 tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc
      901 cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc cttcttctac
      961 cgcagcacgg gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt tcatttcaac
     1021 ttcggtagca gcacctactt tgataactgc aacgccgcct gccagtttcg ctacgcgttc
     1081 ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac ggatctgagc
     1141 aacacggccc tggattgcag cttcttcacc cacgccatcg atgatagtgg tggtgtcttt
     1201 gttgatcaca acacgtttag cctgacccag gtcttccagg gttgcttttt ccagctccat
     1261 accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct gcagcatagc
     1321 tttacgacga tcgccgaagc ccggagcttt aaccgcagca actttcacga tgccacgcat
     1381 ggtgttaaca accagagttg ccagcgcttc gccttcaaca tcttcagcga tgatcagcag
     1441 cggtttgcct gctttggcaa cagcttccag aaccggcagc atttcgcgga tgttagagat
     1501 tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag tttccggctt
     1561 gttgatgaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa ccacgtccag
     1621 ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac cgactttgtc
     1681 catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga tggtaccaac
     1741 ctgagcaatc gctttagagt cagagcacgg tacggacagc gctttcagtt cttcaactgc
     1801 agcggtaacg gctttgtcga taccacgttt caggtccatc gggttcatgc ccgcagcaac
     1861 agctttcaga ccctcagtga tgatagcctg agccagtaca gttgcagtgg tggtaccgtc
     1921 gcctgcagcg tcgttcgctt tagaggcaac ttctttcacc atctgcgcac ccatattttc
     1981 gaacttgtct tccagttcga tttcacgagc aacggaaaca ccatctttgg tgatggtcgg
     2041 tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg taactttcac
     2101 tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt taccgaattt
     2161 tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg tcgtgcgagg
     2221 attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact tcttcattgt
     2281 cgatcttctc agatttcaca ccgtagccat cgttgaaaat aacgatgtcg ccaactttca
     2341 catccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca gccagcactt
     2401 cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca gcagatttag
     2461 tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata ttcattgata
     2521 actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac gtggtttccc
     2581 ggctggtgac cagagaaatg gggatgagga ttcgcccctt caagggggaa aaagaaaaaa
     2641 aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt catcgcaatt
     2701 tcttcatcgc cgtcataagc gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc
     2761 caaaatttgg gcgcaatcgg atatcaaccc atgagtggac tcaaacaaga actggggctg
     2821 gcccagggca ttggcctgct atcgacgtca ttattaggca ctggcgtgtt tgccgttcct
     2881 gcgttagctg cgctggtagc gggcaataac agcctgtggg cgtggcccgt tttgattatc
     2941 ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc cagcgcaggc
     3001 ggtgtcgcgc acttcgtcgg tatggcgttt ggttcgcgac ttgagcgagt caccggctgg
     3061 ttgtttttat cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc cgccgggttc
     3121 ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact cggtacgctg
     3181 gcgctggtgt ggtatatcgg tactcgcggt gccagttcca gtgctaatct acaaaccgtt
     3241 attgccggac ttatcgtcgc gctgattgtc gctatctggt gggcgggcga tatcaaacct
     3301 gcgaatatcc ccttcccggc acctggtaat atcgaactta ccgggttatt tgctgcgtta
     3361 tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg cccatcttgc ctcggaattt
     3421 aaaaatccag agcgtgattt tcctcgcgca ttgatgattg gtctgctgct ggcaggatta
     3481 gtctactggg gctgtacggt agtcgtctta cacttcgacg cctatggtga aaaaatggcg
     3541 gcggcagcat cgcttccaaa aattgtagtg cagttgttcg gtgtaggagc gttatggatt
     3601 gcctgcgtga ttggctatct ggcctgcttt gccagtctca acatttatat acagagcttc
     3661 gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctggc acgcctctct
     3721 tctcgccata tcccgaataa tgccctcaat gcggtgctcg gctgctgtgt ggtgagcacg
     3781 ttggtgattc atgctttaga gatcaatctg gacgctctta ttatttatgc caatggcatc
     3841 tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca aggacgttat
     3901 cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactggcaat ggtcggctgg
     3961 aaaagtctct atgcgctgat catgctggcg gggttatggc tgttgctgcc aaaacgaaaa
     4021 acgccggaaa atggcataac cacataatcc ggcgtttcga cattaatcct ggcgatcgtc
     4081 tttatgatca aggcggtcgc gctcatcatc ctttcgctgg tactcaccat caaaagtatt
     4141 accgccaccg gtcccggcgc taaaaccgcc gcctggcata cgagaaaagc gcaaatgcgg
     4201 catcaacttc actgtcagat gcttttgcac cggcggtaat aaaagcagaa gaccgaggaa
     4261 gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg aaacactttt
     4321 aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca gcacgaaatt
     4381 cttaaagccc tggttacgta ccaatgacat cccgataact gacgtgaata taaccagcac
     4441 gagggtcagc aataccccca atacatgggc aacctgaata aagattgaaa tctcaatata
     4501 gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag
     4561 ccggtaaaac ccggctacct gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat
     4621 tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag taatcccaaa gcggtgatct
     4681 atttcacaaa ttaataatta aggggtaaaa accgacactt aaagtgatcc agattacggt
     4741 agaaatcctc aagcagcata tgatctcggg tattcggtcg atgcagggga taatcgtcgg
     4801 tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa agcaaatcat tggcagcttg
     4861 aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg
     4921 gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat
     4981 atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa
     5041 aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc
     5101 atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta
     5161 gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc
     5221 aatatcggtc tggaactgat gggtcaccag aaaggtgaat atcagtacct gaacccgaac
     5281 gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca
     5341 gtttactctt ccctgattaa gctggtagat gcgattaacc aactgcgtga aggctttgaa
     5401 cgtaaagctg tcgaattcca ggacatcctg aaaatgggtc gtacccagct gcaggacgca
     5461 gtaccgatga ccctcggtca ggaattccgc gctttcagca tcctgctgaa agaagaagtg
     5521 aaaaatatcc aacgtaccgc tgaactgctg ctggaagtta accttggcgc aacagcaatc
     5581 ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa
     5641 gtcactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc
     5701 gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt
     5761 aacgacctgc gcttgctctc ttctggtcca cgtgccggcc tgaacgagat caacctgccg
     5821 gaactgcagg cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg
     5881 gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa
     5941 gcaggtcagc tgcagttgaa cgttatggag ccggtcattg gccaggccat gttcgaatcc
     6001 gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcact
     6061 gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg
     6121 aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt
     6181 aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat
     6241 attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa
     6301 agcgaacagt aatcgtacag ggtagtacaa ataaagaagg cacgtcagat gacgtgcctt
     6361 ttttcttgtg agcagtaact taaaaataac aacctaatat caacttgtta aaaaacaagg
     6421 aaggctaata tgctagttgt agaactcatc atagttttgc tggcgatctt cttgggcgcc
     6481 agattggggg gaataggtat tggttttgca ggcggattgg gggtgctggt tcttgccgct
     6541 attggcgtta aacccggtaa catcccgttc gatgtcattt ccattatcat ggcggttatc
     6601 gccgctattt ctgccatgca ggttgctggc ggtctggact atctggttca tcagacagaa
     6661 aagctgctgc gccgtaaccc gaaatacatc acgatcctcg caccgatcgt gacctatttc
     6721 ctgactatct ttgctggtac tggcaacata tctctggcga cactgccagt tatcgctgaa
     6781 gttgcgaagg aacaaggcgt caaaccttgc cgtccgctgt ctactgcagt ggtatccgcg
     6841 cagattgcga tcaccgcatc gccaatctca gcggcagtgg tttacatgtc ttccgtgatg
     6901 gaaggtcatg gcatcagcta cctccatctg ctctccgtgg tcatcccgtc caccctgctg
     6961 gcggttctgg tgatgtcctt tctggtcact atgctgttca actccaaact ctctgacgat
     7021 ccgatttatc gcaagcgtct ggaagagggc ctggttgaac tgcgcggtga aaagcagatt
     7081 gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt agttggcgtg
     7141 gttatctatg caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc gctgatgaac
     7201 accaccaacg caatcctgat catcatgctc agcgttgcaa ctctgaccac cgttatctgt
     7261 aaagtcgata ccgacaacat cctcaactcc agcaccttca aagcaggtat gagcgcctgt
     7321 atttgtatcc tgggtgttgc gtggctgggc gatactttcg tttccaacaa catcgactgg
     7381 atcaaagata ccgctggtga agtgattcag ggtcatccgt ggctgctggc cgtcatcttc
     7441 ttctttgctt ctgctctgct gtactctcag gctgcaaccg caaaagcact gatgccgatg
     7501 gctctggcac tgaacgtttc accgctgacc gctgttgctt ctttcgctgc ggtgtctggt
     7561 ctgttcattc tgccgaccta cccgacgctg gttgctgcgg tacagatgga tgacacgggt
     7621 acaacccgta tcggtaaatt cgtcttcaac catccgttct tcatcccggg tactctgggt
     7681 gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct tcatgctgta atgaccaatc
     7741 gcggggcgtt caagccccgc tttctttccc gccgactaac atccttcccc cgtccgttgt
     7801 atagtgacct ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc ttgatgaaaa
     7861 aagttcgaat accgcgtctg tcgtggtgct atgtaccgca ccggatgaag cgacagccca
     7921 ggatttagcc gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct tgatccccgg
     7981 cgctacctct ctctattact gggaaggtaa gctggagcaa gaatacgaag tgcagatgat
     8041 tttaaaaact accgtatctc accagcaggc actgctggaa tgcctgaagt ctcatcatcc
     8101 atatcaaacc ccggaacttc tggttttacc tgttacacac ggagacacag attacctctc
     8161 atggctcaac gcatctttac gctgatcctg ctactttgca gcacttccgt ttttgccgga
     8221 ttattcgacg cgccgggacg ttcacaattt gtccccgcgg atcaagcctt tacttttgat
     8281 tttcagcaaa accaacatga ccttaatctg acctggcaga tcaaagacgg ttactacctc
     8341 taccgtaaac agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt gcagctgccg
     8401 caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg agatttaccg cgatcggctg
     8461 acgcttcccg taaccatcaa ccaggcgagt gcgggagcga cgttaactgt cacctaccag
     8521 ggctgtgctg atgccggttt ctgttatccg ccagaaacca aaaccgttcc gttaagcgaa
     8581 gtggtcgcca acaacgcagc gtcacagcct gtgtctgttt cgcagcaaga gcagcacacc
     8641 gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg gtattggtat cgcctttacg
     8701 ccatgcgtgc tgccaatgta cccactgatt tctggcatcg tgctgggtgg taaacagcgg
     8761 ctctccactg ccagagcatt gttgctgacc tttatttatg tgcaggggat ggcactgact
     8821 tacacggcgc tgggtctggt agttgccgcc gcagggttac agttccaggc ggcgctacag
     8881 cacccatacg tgctcattgg cctcgccatc gtctttacct tgctggcgat gtcaatgttt
     8941 ggcttgttta ctctacaact cccctcttcg ctgcaaacac gtctcacgtt gatgagcaat
     9001 cgccaacagg gcggctcacc tggcggtgtg tttgttatgg gggcgattgc cggactgatc
     9061 tgttcaccat gcaccaccgc accgcttagc gcgattctgc tgtatatcgc ccaaagcggg
     9121 aacatgtggc tgggcggcgg cacgctttat ctctatgcgt tgggcatggg cctgccgctg
     9181 atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg gatggaacaa
     9241 gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg tcttcctgct ggagcgagtg
     9301 attggtgatg tatggggatt acgcttgtgg tcggcgctgg gtgtcgcatt ctttggctgg
     9361 gcctttatca ccagcctaca ggctaaacgc ggctggatgc gcgtggtgca aataatcctg
     9421 ctggcagcgg cattggttag cgtgcgccca cttcaggatt gggcatttgg tgcgacgcat
     9481 accgcgcaaa ctcagacgca tctcaacttt acacaaatca aaacggtaga tgagttaaat
     9541 caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag atctttatgc cgactggtgc
     9601 gtcgcctgta aagagtttga gaaatacacc ttcagcgacc cgcaggtgca aaaagcgtta
     9661 gcagacacgg tcttacttca ggccaacgtc acggccaacg acgcacaaga tgtggcgctg
     9721 ttaaagcatc ttaatgtcct tggcctaccg acaattctct tttttgacgg acaaggccag
     9781 gagcatccac aagcacgcgt cacgggcttt atggatgctg aaaccttcag cgcacatttg
     9841 cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa tagcgtgcaa
     9901 cgtgaagatg tactgggaga agccctgaaa ttattagaat tacaagggat tgccaacacc
     9961 acgctggaga tggttgctga acgtgtggat tatccactgg acgagctacg ccgcttctgg
    10021 ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca gatagatgtc
    10081 tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg aacaaaagct gctggcacgt
    10141 tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc cgggctgtct atttatcgct
    10201 gcctgtacgt tttatcccga tcctggccac cctattcatc aactggccga tcagcaaaaa
    10261 agcgcggcct acgatttcac ccacgaactg ttaaccacac tggaagttga cgatccggcg
    10321 atggtagcaa agcagatgga actggtgctg gaaggctgtt taagccgaat gctggtgaat
    10381 cgtagccagg cggatgtcga cactgcacat cggctggcgg aagatatcct gcgcttcgcc
    10441 cgctgccgcc agggtggtgc attgacctga cagaaacaca gaaaagaagc gatttgccgc
    10501 aatcttaagc agttgaatcg cttttactga aattaggttg acgagatgtg cagattacgg
    10561 tttaatgcgc cccgttgccc ggatagctca gtcggtagag caggggattg aaaatccccg
    10621 tgtccttggt tcgattccga gtccgggcac caaattcata taaacggacc tccacggagg
    10681 tccgtttttc gtttcaggac gccacgattt aagcgttctg cctccaaatc aattctcccg
    10741 aactcaacca gattctcccc tcatcaccag caatttgcgg gcatatccca attcgggaaa
    10801 atttgttcct gagcgatagc actaactgac gtgaaatgtc gtgcggcccc gtgatgttgt
    10861 tgaacgtcaa atgacgtcat caggagcgta acgcatccat aaagcacaac accggtctga
    10921 acgccgactg atgagatttt ctgaatgaga acaaagagaa atgtatcagt ccgcttgctc
    10981 atgcaaagac taacaatcca ttaaaatagt aagcgctccg gacaattttc catggattat
    11041 tttctgaaca tttttctttg gcaaagatga tgaattttga tggtaaggaa tattacttct
    11101 ggttctcagt aaaatcc
//
LOCUS       DAFAVF010000135        11078 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFAVF010000135 DAFAVF010000000
VERSION     DAFAVF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.135"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            32..202
                     /locus_tag="LGK42_003479"
     CDS             32..202
                     /locus_tag="LGK42_003479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659694.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(260..1033)
                     /gene="zupT"
                     /locus_tag="LGK42_003480"
     CDS             complement(260..1033)
                     /gene="zupT"
                     /locus_tag="LGK42_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBK2659695.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1176..1964
                     /gene="ygiD"
                     /locus_tag="LGK42_003481"
     CDS             1176..1964
                     /gene="ygiD"
                     /locus_tag="LGK42_003481"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBK2659696.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(2002..3162)
                     /locus_tag="LGK42_003482"
     CDS             complement(2002..3162)
                     /locus_tag="LGK42_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBK2659697.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(3168..3839)
                     /locus_tag="LGK42_003483"
     CDS             complement(3168..3839)
                     /locus_tag="LGK42_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBK2659698.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(3987..5468)
                     /gene="tolC"
                     /locus_tag="LGK42_003484"
     CDS             complement(3987..5468)
                     /gene="tolC"
                     /locus_tag="LGK42_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBK2659699.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            5673..6302
                     /gene="nudF"
                     /locus_tag="LGK42_003485"
     CDS             5673..6302
                     /gene="nudF"
                     /locus_tag="LGK42_003485"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBK2659700.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6303..6725
                     /locus_tag="LGK42_003486"
     CDS             6303..6725
                     /locus_tag="LGK42_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBK2659701.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            6750..7577
                     /gene="cpdA"
                     /locus_tag="LGK42_003487"
     CDS             6750..7577
                     /gene="cpdA"
                     /locus_tag="LGK42_003487"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBK2659702.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            7577..8158
                     /gene="yqiA"
                     /locus_tag="LGK42_003488"
     CDS             7577..8158
                     /gene="yqiA"
                     /locus_tag="LGK42_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBK2659703.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            8187..10079
                     /gene="parE"
                     /locus_tag="LGK42_003489"
     CDS             8187..10079
                     /gene="parE"
                     /locus_tag="LGK42_003489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK2659704.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10124..10993)
                     /locus_tag="LGK42_003490"
     CDS             complement(10124..10993)
                     /locus_tag="LGK42_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HBK2659705.1"
                     /translation="MNNKFKIILWMILSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
BASE COUNT         2619 a         2715 c         2954 g         2790 t
ORIGIN      
        1 tttagtattg ttaaatatta agggtaaaag gatgtttatc gcctggtact ggattgtatt
       61 gattgctctt gttgtggtgg gttatttcct gcatttgaaa cgttattgtc gggcgtttcg
      121 ccaggacaga gacgcactgc ttgaagcgcg gaacaaatac ttaaacagta cgagagaaga
      181 gacagccgaa aaggtagaat aaggctttag tgaagctagc agaagttgtc catgcgctgg
      241 ccaacttctg atgttgcttt taaccaattc ccgccgtttg tagcagcact aaactgaatc
      301 ccatcactga cattccacat agtacgccat agctggggtt attattaggg tcaatctctt
      361 tggcgagcgg cattaattca tcaaccgaga gcgccaccat aattcctgca accgccgcca
      421 tgattgccgc catgaccacg ggggaaatca tgctaccgag gattaaccac gccagcacac
      481 caccaagaat ttctgccaat ccagaaatcc ccgcccacag aattgccgtg cgtttagaac
      541 ccgttgctgc ataaaccggg cctgcaaccg ccagaccttc aggaatattg tgcaacgcga
      601 cggccagtgc gatgccaaat cccagctcca ggttgctgct cgccgtgaca aaggtggcaa
      661 tcccttccgg gaagttatgc agactgatgc cgagagtgag cagaatggct gtgcgcttga
      721 tcgattttgg caacggctgc accgattttt gcattaaatc ctgcggatgc gcatgtggca
      781 acatgcggtc caggccaaaa tagccaagca gaccgaagat aaacattcca taacccaaca
      841 caggcgacat tccttcagct gctagtgcgg caggaagcat ttccattaat gagatgagca
      901 acatgatccc cgccgcaaat cctagcgaaa acgccagtaa gcggttcgag ggtttttgcc
      961 cgagaacgcc gagaaacgcg ccaataaacg tggctgcccc cgccagtatg gtcagaatga
     1021 gaggtactga catcgacaac tccttataaa tcttcacctt caggcagatc gtcaaaataa
     1081 ctgatgatca tcatcattgt tatccgggtt cttactccgc cgaacaagcg tattgtgagg
     1141 atatcaaaat gacaccttta gttaaggata tcatcatgtc ttcaacacgt atgccagcat
     1201 tgtttttagg tcacggtagt ccgatgaacg tgctggaaga taatttgtat acccgcagct
     1261 ggcagaagtt ggggatgaca ttgccacgcc cgcaagcgat tgtggtggtt tcggctcact
     1321 ggtttacccg tggaacagga gtgaccgcga tggagacgcc gcccacgatt catgactttg
     1381 gtggcttccc gcaggcgctg tacgatacgc attatcctgc tccgggttcg cctgtgctgg
     1441 cacagcatct ggttgagctg ttagcaccgg tcccggtgac gctggataaa gaagcctggg
     1501 gctttgacca cggttcctgg ggcgtgctga ttaagatgta tcctgacgcc gatatcccga
     1561 tggtgcagtt gagtatcgac agtagcaaac ctgccgcctg gcatttcgaa atggggcgca
     1621 aactggcagc gctgcgagat gaaggaataa tgttggtcgc cagtggcaac gtggtgcata
     1681 acctgcgtac agtgaagtgg cacggtgata gttcaccata tccttgggcg atgtcgttta
     1741 atgagtatgt gaaagcgaat ctgacgtggc aaggggcggt agagcagcat cctctggtga
     1801 attacctcga ccatgaaggt ggcgcgttat cgaacccaac gccggagcac tatctgccgt
     1861 tgttgtatgt cttaggtgcg tgggatgggc aggagccaat taccattccg gtcgatggta
     1921 tagaaatggg cagcctgagt atgctgtcgg tgcagatagg ttaacaatta ccggatggcg
     1981 aatgccatcc ggcatcattg attactcaac aaaaatatgt ggataaaacc gagacatatc
     2041 ctgggtgatc aatgcacggt cttcacgaat gccaattccg gcgggttgat cgttcaccag
     2101 ccagctacca atcagcatat agctgtcgcc gaatttcggt aacgggtgga attgctgaac
     2161 aatcatccct tcttcgccat acggaccttc cgctgcttca atggttttgc cgttttcaat
     2221 gatcgacacg tttgcgcctt cacgggagaa gatcggttta accacatatt tttccatttg
     2281 cggatgatca tcttccgcaa aataagcggg cagcaggttc gggtgattcg ggaacatctc
     2341 ccacagtagc ggtagaagtg ccttgttgga gataatgctc ttccacgctg gttccagcca
     2401 gcgtacgcct gcatcctcca gcttggtgga aaacatctca cgcaacataa attcccacgg
     2461 atacagcttg aacaggttgg aaattacctg atcctgtaaa tccgtgaact gacctttttc
     2521 acctaacccg atatcatcga tgtagaggaa ctcagtagca atttcagctt ccgttgcgca
     2581 gtcctgcaaa tactgaatgg ttccgcgatc ttccaccgtg tcgcgacagc aggtgagatg
     2641 cagcaactgg aagccatact gttcacgcag ctcaacgaag cgatcgatca gtttttcttg
     2701 cagactgtta aactggtcgc tgccctccgg caagttaccg gcgttaagct gatcttccag
     2761 ccagatccac tgaaagaacg ccgcctcgta tagtgacgtt ggcgtatcgg cgttattttc
     2821 cagaagttta ggttcgccag tgccatccca cgccagatca agacgcgaat aaagcgatgg
     2881 ctggtgcgtc agccatgact ggcgcacaaa actccaggtg tgttttggaa tgcggaattt
     2941 ggtcatcagc tcatcgctgg cgatcacttt ttccaccact ttcaggcaca tctggtgcag
     3001 ttcggcggtg acttcttcca gcttttcaac ctgggcgagg gtcaacttgt agtaagcatc
     3061 ttcacaccag tacggctcgc cgtacatggt gtgaaaattg aaaccgtatt cgtgggcttt
     3121 ctcacgccag tccgggcgct cggtaatact gactctttcc atcggtatca gccacccatt
     3181 gaacgagaag aggtgccagt tgcgctacgc tgcatagtgc tttgtttggc aacagattca
     3241 ccaaaaccgc cacgggtaac ggtagtggtg gtcgccggtt ttggtgccat ggctgtcttc
     3301 ggtacggtca tggtgcggcc tggctgggct gcgccatagt ttttacccgt cgcgtcggta
     3361 tatttaccgt aagccgggct ggctgggttt ttcgaggaga acagcggctg ctgtgcaaat
     3421 cccgcgccgc cgcccatcag acgccccatc atgtaaccgg ccatcagcgg catccagaaa
     3481 ctcccgctgg attgctgggc ctgcgcctgg ttttctggtg ccatgccagc ctgggctggt
     3541 gcctgctggc actgaccttc accaaattca gcaacacagt cttcacgggt ggcgtatttc
     3601 ggcgcagtac gttcggcttc tttcagcgca ttgttgtacg cggtggtaca ttcggcgctt
     3661 ttgcctgggt ttgcagctga acagtcgtca gcattttgat agagagacac tgtttcatca
     3721 ctcttttcac agccagccag cataaaaaca gtggcaaccg cgagagcgac tggtgtcaga
     3781 tggcgcgcgc tccagttttt gcggaacgat gcgtggcgta tggattttgt ccgtttcatt
     3841 tttgtcttcc gggaccagtg gtaaataccc atcagaatag aggatggctg gtcgaaattg
     3901 aagcgagaag ggggaagaat gcggcagata acccgtatct ttacgttgcc ttacgttcag
     3961 acggggccga agccccgtcg tcgtcatcag ttacggaaag ggttatgacc gttactggtg
     4021 gtagtgcgtg cggatgtttg ctgaacgacg ggtgccgggc tatcaggcgc ataaccatca
     4081 gcaatagcat tctgttccgg cgtttgcggg gcaacgtttt ccggattagt ggaaaccggt
     4141 ttgctcagcg cattgttcag tgccagcaga tcctgctcgt tcaacgtacc cagggctgac
     4201 ttaatattca gctgattaat aaggtagtta taacgcgcat ttgccagctc ttgcttagcg
     4261 ttgtacagcg tggtggttgc atccaacaca tcaacaatgg tacgcgtacc gaccgagtag
     4321 cccgcttcca tcgcgtctaa tgagctttga gcggaaacta cggcttgttt gtaggcgtta
     4381 atgctactga tagatgcatt aatgttgttg aaggaggaac gtacggtttg cacgacgcta
     4441 cgatgcgcgc tttccagttg ctcgctggca ccaacaaagt tgtactgggc ctgtttcacc
     4501 tgcgagttaa ccattccgcc ctgataaatc ggcagcgaga agctcaggcc cactttgttc
     4561 tggcccatat tgctgtcgtc atactgggta ccagcggcac cacgagtttt cgaaccgcta
     4621 taagaggtgt cagaaatccc ggtagaagcc gttaaatcca gcgtcggtaa gtgaccatcc
     4681 tgcgcctggc gaatttgctc gcgcgccagg tcctggctca agcgtgcctg taacagcgac
     4741 aggttgcgtt tttcggcttc tttcagcagc gcgttaaccg gctgtggttt gtcggtttta
     4801 aagttttcga cattcagcgc tgccagttct ggatagtagt taccggtgat ctggcgcagc
     4861 tgctctaccg cgttatcaag gttattacgt gcggtcactt cgttcgccag cacggtatcg
     4921 tactgcgcgc gggcgttctg cacgtcggtt atcgctacca ggcccacgtt aaaacgttgg
     4981 gtggtttgat ctaattgacg gtagatcgct tctttttgtg cctgtgtata ggaaagaacg
     5041 tcaatagcat tcaacacgtt gaaataagcg gtcgcggtgt tgaggatcaa ggtttgctga
     5101 tcggtctgat atgtgacgtc ctgaatccct gctgcttttt cctgcagcgt taacgcacgc
     5161 catttcgaca tatcaaaaat ggattgagtt aactgcaggg acgcactggt cgcgttagag
     5221 ttgatgccgt tcgcgtcgcg gtagccgttg ctataggtgt aatctgcacc taaacctagc
     5281 tgtggcagta atggactgcg cgcttcatta attttttcaa aggcagcatc acgatcggcg
     5341 gcagacttac gcaattccgg gttactaagg cgtgcttgct gataaacttg catcaggttc
     5401 tcggcctggc tcaacgaact gaacccagaa aggctcaggc cgataagaat ggggagcaat
     5461 ttcttcattt gcattccttg tggtgaagca gtatttagcg cgatcaaact gtaaaattat
     5521 tgcggattct agcagaagcc gctaccgcaa aaggttggcg ttacgtgcca tagggcgtta
     5581 atgtgcctca atttaacaca tgactgctca aacggcagtc aaacgtcgct gaaattcaca
     5641 tttaattcac tattagtgcc aggacattaa caatgcttaa gccagacaac ctgcccgtta
     5701 catttggcaa aaacgatgta gaaattattg cacgagaaac actttatcgc ggcttttttt
     5761 cattagatct ttatagattt cgtcatcgtt tattcaacgg gcaaatgagt catgaggtac
     5821 ggagggaaat ttttgagcgc ggtcacgccg cagtcttgct accctttgac ccagtgcgtg
     5881 atgaagttgt gctgattgag cagattcgga ttgccgcata cgacaccagt gaaaccccct
     5941 ggctactgga gatggttgcc gggatgatag aagagggtga aagtgtggaa gatgttgccc
     6001 gtcgcgaagc gattgaagag gcgggactga tagtcaaacg gaccaaaccg gtgttaagtt
     6061 tcctggcaag cccggggggc accagtgagc gttcgtcaat tatggtgggc gaagtggacg
     6121 ccacgaccgc aagcggtatt catggtctgg ctgatgaaaa cgaagatatt cgcgttcatg
     6181 tggtaagccg ggaacaggca taccagtggg tagaagaggg gaaaatcgac aacgcagcgt
     6241 cggtcatcgc tttgcaatgg ctgcagctgc atcatcaagc gttaaaaaat gagtgggcat
     6301 aaatgaagcg ttacacacct gactttcctg aaatgatgcg cctgtgcgag atgaactttt
     6361 cacaattgcg ccgtttgtta ccgcgcaatg acgcacccgg cgaaactgta agctatcagg
     6421 tggcaaacgc acaatatcgg ctgacgattg tggaatcgac ccgatacact accctggtga
     6481 caatagaaca gactgcgccc gcgatcagtt actggagcct tccgtcaatg acggtgcgtc
     6541 tgtatcatga cgcgatggtg gctgaagtgt gttcaagcca gcagattttt cgcttcaaag
     6601 cgcggtatga ttatcctaat aaaaagttgc atcaacgcga cgaaaagcat caaattaacc
     6661 agtttttagc ggactggttg cgatactgtt tagcacatgg agcgatggcg attccggttt
     6721 actagcgtcg tgaaacctaa ggacaccatt tggaaagcct gttaaccctt cctctggctg
     6781 gtgaggccag agtcaggatt ttacaaatta ccgatactca cctgtttgca caaaagcacg
     6841 aagccctgtt aggggtaaac acctgggaaa gctaccaggc ggtgctggag gcgattcgtc
     6901 cacatcagca cgaattcgac ctgattgtcg cgacaggtga tttagcgcag gatcaatcct
     6961 ctgcggccta tcagcatttc gctgaaggca tcgcaagttt tcgtgcgccc tgcgtctggc
     7021 tgccgggcaa ccacgatttc cagcccgcga tgtacagcgc gttacaggat gcgggtatct
     7081 ccccggcgaa gcgcgtgttt attggtgagc aatggcaaat cctgttgctg gatagccagg
     7141 tgtttggcgt gccgcacggt gagctgagcg agtttcagct tgagtggctg gaacgtaaac
     7201 tggccgatgc gccagaacgc catacgttgc tgctgctgca tcatcatccg ctacctgcgg
     7261 gttgtagttg gcttgatcaa cacagcctgc gtaacgcggg cgaactggat accgtgctgg
     7321 cgaagtttcc gcacgtcaaa tacttgctgt gcggtcatat tcatcaggag ctggatctcg
     7381 actggaacgg tcgccgcctg ctggcaacgc cgtcgacctg tgtgcagttt aagccgcact
     7441 gttccaactt tacgctggac accatcgcgc ccggctggcg tactctcgag ttacatgctg
     7501 atggcacgct gaccaccgag gtgcatcgcc tggcggacac acgtttccaa cctgataccg
     7561 cttcagaagg ctactgatgt ctacgcttct ttatttacac ggtttcaaca gctcgccgcg
     7621 ctctgcaaaa gcgagcttgt taaaaaactg gctggcggaa catcaccctg acgttgagat
     7681 gatcattccg cagttgccgc cgtatccttc cgacgcggca gagctgctgg aatccattgt
     7741 cctggaacat ggcggcgatt cgctgggtat tgtcggttcg tcactggggg gatattacgc
     7801 cacctggttg tcacaatgtt ttatgctgcc cgcagtggtg gtaaacccgg cggtgcgccc
     7861 gtttgaactg ctgacggact atctcggtca gaacgagaac ccctacaccg ggcagcaata
     7921 tgtgctagag tcacgccata tttacgatct caaagtcatg cagattgacc cgctggaagc
     7981 gccggatttg atctggctgc tgcaacagac gggagatgaa gtgctggatt accgccaggc
     8041 ggtggcgtac tacgcttcct gccgccagac tgtcatagaa ggcggcaacc acgcattcac
     8101 gggcttcgaa gattatttca acccgatcgt cgattttctt ggtctgcacc atctctgacg
     8161 atcaactcga attgctaact taaaccatga cgcaaactta taacgctgat gccattgagg
     8221 tactcaccgg gcttgagccg gttcgccgcc gtccggggat gtataccgat accactcgcc
     8281 ctaaccattt ggggcaagaa gttattgata acagtgtcga tgaagcactg gcgggtcacg
     8341 caaaacgcgt ggatgtaatc ttacatgctg accagtcgtt agaagttatt gacgatgggc
     8401 gcgggatgcc ggtggatatt cacccggaag agggtgtacc ggcggttgaa ctgattcttt
     8461 gccgtctgca tgcaggcggt aaattctcta acaaaaatta ccagttctct ggcggcctgc
     8521 atggcgtggg gatttcggtg gttaacgccc tgtcgaagcg cgtagaagtt aacgtgcgcc
     8581 gcgatggtca ggtttataac atcgcctttg aaaatggcga aaaggtgcag gatttacagg
     8641 ttgtcggcac ttgcggtaaa cgcaataccg gtaccagcgt gcacttctgg ccggatgaaa
     8701 ccttctttga cagcccgcgt ttttctgttt cacgcctgac gcatgtgctg aaggccaaag
     8761 cagtactgtg ccccggcgtt gagattacct ttaaagatga gatcaacaac accgaacagc
     8821 gctggtgcta tcaggacggt ctgaatgatt acctggcgga agcggtaaac ggtttaccga
     8881 cgctgccaga aaaaccgttt atcggtaatt tcgctggcga tactgaagct gtggactggg
     8941 cgctactgtg gctgccggaa ggcggtgaac tgctgaccga aagctacgtc aaccttatcc
     9001 caacgatgca gggcggtacg cacgttaacg gtctgcgtca gggcctgttg gacgcgatgc
     9061 gtgagttctg tgaataccgc aacattctgc cgcgcggtgt aaagttgtcg gcggaagata
     9121 tctgggatcg ctgcgcctat gtgctgtcag taaaaatgca ggatccgcag tttgccgggc
     9181 agaccaaaga gcgtctctct tcgcgtcagt gcgcggcatt tgtttctggc gtggtgaaag
     9241 atgcctttat cctgtggctg aaccagaacg ttcaggcggc ggagttgctg gcggagatgg
     9301 cgatttccag cgcccagcgc cgtatgcgtg cggctaaaaa agtggtgcgc aaaaagctga
     9361 ccagcggccc agcactgcct ggcaaactgg ctgactgtac cgcgcaggat cttaaccgta
     9421 ccgaactgtt ccttgtggaa ggtgactccg caggcggatc tgccaagcag gcgcgcgatc
     9481 gcgaatatca ggcgatcatg ccactgaaag gtaagatcct taacacctgg gaagtttctt
     9541 ccgacgaagt gctggcctcg caggaagtgc atgatatttc ggtagcgatc ggtatcgatc
     9601 ctgacagcga cgatctgagc cagcttcgtt atggcaaaat ctgtatcctc gcggatgcgg
     9661 actctgatgg tctgcacatt gccacgctgc tctgcgcttt gttcgtaaaa cacttccgcg
     9721 cgttggtgaa acacggtcac gtttacgtcg cactgccacc gctctaccgt attgatctcg
     9781 ggaaagaggt ttattacgcg ctgacggaag aagagaaaga gggcgtactt gagcaattaa
     9841 aacgcaagaa aggcaagccg aacgtccagc gttttaaagg tctgggggaa atgaacccga
     9901 tgcaattgcg tgaaaccacg cttgatccga acactcgccg tctggtgcag ttgactatcg
     9961 atgatgaaga cgatcagcgt actgacgcga tgatggatat gctgctggcg aagaaacgct
    10021 cggaagatcg ccgcaactgg ttgcaagaga aaggcgacat ggcggagatt gaggtttaat
    10081 aatctttacc cggcaaacat gtgttttgcc gggttgtcgc tgattatttt attgggcaca
    10141 gtttgttacg atctggaaat agtgttgcca gcgaaccagc cagagttttc cagtccgtag
    10201 ccattttttc tgcttgtgca gttgttatta ctgcaccttg ttgtttaata agctcatcct
    10261 ccactctggt atagtgaata atggtaaaat ccgtagagga ccatttatcc ttagagaaca
    10321 tcttcgtcat cgcatgaatg tttgcttgcc cgtcatcgat aaaaacaatc gcgtcatatt
    10381 tgttccccat tttatccagc atataatcga gaattacgcc tttatcttgc ccggaagaaa
    10441 aaaatacccc ctggctgtac agccatgacc gtttgagttt ccctttttca aagggtggca
    10501 aagtatcctc tttcttcctg agtgcggaag acgaaaaatc aatgccgttt cgctttaatt
    10561 cccgcaatgt cggaaagaga gtatccggtg cacgggatgt cagagcaaaa gcggtatgct
    10621 tttgttgcca ctgttttaac attgtcggta actgtggctc ggtaagctgc atagggctaa
    10681 gttcgtaaag catactgatt gtattttcat acaggcaggg aactttttgc gagtcatccg
    10741 gctttaaggg caatttatcg gtttgccatt gataccagat atcaccgcca atgcgtgtgc
    10801 cactcgttaa taacgtatta tcaatgtcaa aaaccaataa tatcttatcg gatggataga
    10861 gagtttcctt ttcggtgata attttactga ccgcactata ttcgccagta actatatttt
    10921 ttacctgtgc gtaagataaa gctggcagag cagagagtat catccagaga atgattttaa
    10981 atttattatt cattttttat tccagattag aaagtataag tcagacccca gcgccagcgg
    11041 atttggcgat tacttgatgt tttatccaca cctttata
//
LOCUS       DAFAVF010000136        11046 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFAVF010000136 DAFAVF010000000
VERSION     DAFAVF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.136"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            89..1291
                     /gene="nupC"
                     /locus_tag="LGK42_003491"
     CDS             89..1291
                     /gene="nupC"
                     /locus_tag="LGK42_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBK2659706.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(1340..3529)
                     /locus_tag="LGK42_003492"
     CDS             complement(1340..3529)
                     /locus_tag="LGK42_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBK2659707.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(3738..3813)
                     /locus_tag="LGK42_003493"
     tRNA            complement(3738..3813)
                     /locus_tag="LGK42_003493"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3778..3780),aa:Ala,seq:ggc)
     gene            complement(3853..3928)
                     /locus_tag="LGK42_003494"
     tRNA            complement(3853..3928)
                     /locus_tag="LGK42_003494"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3893..3895),aa:Ala,seq:ggc)
     gene            4149..4508
                     /locus_tag="LGK42_003495"
     CDS             4149..4508
                     /locus_tag="LGK42_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBK2659708.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4510..4902
                     /locus_tag="LGK42_003496"
     CDS             4510..4902
                     /locus_tag="LGK42_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBK2659709.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(4954..6369)
                     /gene="gltX"
                     /locus_tag="LGK42_003497"
     CDS             complement(4954..6369)
                     /gene="gltX"
                     /locus_tag="LGK42_003497"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK2659710.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            6628..6703
                     /locus_tag="LGK42_003498"
     tRNA            6628..6703
                     /locus_tag="LGK42_003498"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6661..6663,aa:Val,seq:tac)
     gene            6748..6823
                     /locus_tag="LGK42_003499"
     tRNA            6748..6823
                     /locus_tag="LGK42_003499"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6781..6783,aa:Val,seq:tac)
     gene            6870..6945
                     /locus_tag="LGK42_003500"
     tRNA            6870..6945
                     /locus_tag="LGK42_003500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6903..6905,aa:Val,seq:tac)
     gene            6950..7025
                     /locus_tag="LGK42_003501"
     tRNA            6950..7025
                     /locus_tag="LGK42_003501"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6983..6985,aa:Lys,seq:ttt)
     gene            7056..7181
                     /locus_tag="LGK42_003502"
     ncRNA           7056..7181
                     /ncRNA_class="other"
                     /locus_tag="LGK42_003502"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7290..8174)
                     /gene="xapR"
                     /locus_tag="LGK42_003503"
     CDS             complement(7290..8174)
                     /gene="xapR"
                     /locus_tag="LGK42_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBK2659711.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(8215..8373)
                     /locus_tag="LGK42_003504"
     CDS             complement(8215..8373)
                     /locus_tag="LGK42_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HBK2659712.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(8426..9682)
                     /gene="xapB"
                     /locus_tag="LGK42_003505"
     CDS             complement(8426..9682)
                     /gene="xapB"
                     /locus_tag="LGK42_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBK2659713.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(9742..10575)
                     /gene="xapA"
                     /locus_tag="LGK42_003506"
     CDS             complement(9742..10575)
                     /gene="xapA"
                     /locus_tag="LGK42_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBK2659714.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            10824..>11046
                     /locus_tag="LGK42_003507"
     CDS             10824..>11046
                     /locus_tag="LGK42_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659715.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGF"
BASE COUNT         2988 a         2846 c         2551 g         2661 t
ORIGIN      
        1 attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa
       61 tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact
      121 tgccgttgtt gcgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg
      181 ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga
      241 cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc
      301 caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt
      361 cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat
      421 tcgcgtgttg ccggtgatca tccgcgcaat tggtttcctg ctctccaaag tcaacggcat
      481 gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt ctgaaaactt
      541 tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc
      601 cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc
      661 gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct
      721 gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga
      781 aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat
      841 tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt
      901 tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc tgggctacat
      961 cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag
     1021 tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat
     1081 tgcttccacg ctctctccgc gtgctgaagg cataatctct gtgttcctgg tttccttcgc
     1141 taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg
     1201 taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg tgagtgtgct
     1261 gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc ggggatcatt
     1321 cccggctttt ttacgcctgt taatcagcta atggctgcgg gcgaccaatc aaataccctt
     1381 gcagatattg caccccgagc ttatgcaata gcgcctgctg ctgttgcgtc tcgacaaact
     1441 ccgcgaccac actcaatgac ttcgctttcg ccagatcggt aattgatctc acaatcatcg
     1501 catccagcgt gttcgtgaca atatctttca caaagacgcc atcaattttg atgatatcag
     1561 cctgcaaacg ctttaaccgt tcgtagttgg catatccggt gccaaagtca tcaatcgcaa
     1621 tccggaaacc aaacttatgc agctgctcga tgttgtacat gctgctttct gcgttagaaa
     1681 acgcctgctc ctcggtgatc tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa
     1741 acagacgaat aatccgcccg gcaatattct tttgcagcag cgtgagcggc attaaattga
     1801 ctgaaaagcg cggtcctttt ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt
     1861 ccagcacttg caaatcaaaa cgcgcgctaa ggttaaactg agcaataagg ggcagaaact
     1921 tatccggggt cataatgccg ccgtcatatt tcagtcgcgc gaggatctca tcataacctt
     1981 caccctcttt gttgcgaatt ggctgggcgt agagcagcaa atcaccctga tctaacgccg
     2041 tgcgaatggt attcagcaat agcacctgtt tagtggtctg cccggaaacc atctcctctc
     2101 tgctatccag cgccagcaca tgatgatgtg cgcaggattg ctccgccagc cagcttaact
     2161 gccccaacaa gggttgcagg gtttcctgat ttccatcaaa acgcccccag gcagcaccat
     2221 agcccatatc cagcccggta ttgttccagt gaatttgccg actattcagg atgttaacca
     2281 tatgctggag tcgcccttcc gtttccggcc cgctcagcac taacagcagt tcactacccg
     2341 gcaattgata caacttttcg ttttcctgca tcaacggcag cagcgtacgg caaattgagc
     2401 ggatacagtg aacgcgcatc attaagccgt aatgacgact cataaactca agattatcaa
     2461 tgcgcaggca gcaaaaactc ttgcccgcct cttgctccgg cgcttgttcc aacgcacgaa
     2521 agttgggtag gagcgttaac ggatctgtca gcgcctgcaa atgccagcgg cgattaagcc
     2581 attcactgcg atgataaatg cgcaccatat agagcaggca aacgctaaag gaaatcagca
     2641 ccgcaagaat aaatgccagc gaatattcgg tttccacccc ttgcaaaaag ttctggttgt
     2701 aattcagcag gcaaagcgtt gaaacagccc aggtgagatt taaaaacgga tagcgaagct
     2761 taccgacccc gagggtaaag atgatgaaga aaacgggtac caagtaaccg gcaataaagt
     2821 cgttttcata aggtgtgcac aacagaagta gcagcacgct taacgctgcc agccagctta
     2881 aggtaaatgc gcgtttctct ttgcccaacg acggagcgat atccctgcgc cacaatatct
     2941 gcgcaaagtg gggacttaca atcatgcgag tgagatagta gaaaagcatg ttgtaaatca
     3001 gcacagcggt gaaaaggctt agcaaatcaa cgaccgtgaa aatggcatcc gcatcgccga
     3061 aaaaggtgga tatctttagc gggaaatcaa agaagctccc cacaagatac atgctgcatt
     3121 tgatgccgat cggcgtcacc aaaccaagcc agaacagacg ttgccagaca tatcggctgg
     3181 tcaatccata acgccaacgc gtccccagct gccagcgtaa tattgcgcag gcacacaaca
     3241 cgacaaacgt ctggctaccg agcatgaccg cagtctgtaa aaaagagaga tgaaagttcc
     3301 acagattggt gcaaaacatc ccgcataaaa cgggaacgac accgcgccag ccaaagataa
     3361 acaagattga cagcattacg cacagtggca tccacgccag aaagatataa ctggaatgga
     3421 taatggcaag tggcgaaata aaacgggata gctgaataag taccacggtg agcgtaaacg
     3481 ctagtgtgaa tatcttgata ttttttatca ggttatgctc cacaaacatg agtacactac
     3541 tctcactgat cggggttttc aggcacacaa ataataagtt tattcgcatc gcgaagcaaa
     3601 cagggggcca gcctgttgcc gatttttcat ccaatgatgc tttcaccagg cggcactaac
     3661 ccggacgtag actgtattac aaaagcggca aaaagcagag acaaaaaacc cccgctttgc
     3721 agcgagggtt ggaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
     3781 atgcaagcgc tctcccagct gagctatagc cccacgatgc gtttacgtac caagtttgct
     3841 gggtgcaaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca
     3901 agcgctctcc cagctgagct atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc
     3961 atcatatgaa ttccgcccga atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct
     4021 gaaaaatcag gcaaatgaac aattttggaa aaccgctcgc attcagtagt tattcatgtc
     4081 acggtttcct gtaaagtggt gttataaaat gaactactaa tagacccaca tacattcagg
     4141 gaattgttat gttcaaggag aggatgacac cagatgaact tgccagactg accggctata
     4201 gccgccagac cattaataaa tgggtacgca aggaaggctg gacaacgtca ccaaaacctg
     4261 gcgtccaggg tggcaaagcc cgactggttc acgtcaatga acaggttcgt gaatatattc
     4321 gcaatgctga acgtccagaa ggtcagggag aagcgcctgc cctttctggt gatgcgccgc
     4381 ttgaagtatt gctggtgaca ctggcgaaag agatgacgcc agttgaacaa aaacagttta
     4441 catccttgct tctgcgggaa gggattatcg gattgttaca acgcttaggg attcgcgata
     4501 gcaaataata tgaaaagatt acgcaacaaa atgaccaccg aagaactggc tgaatgcctc
     4561 ggtgtggcta aacaaactgt taaccgttgg atcagagaaa aaggctggaa aacggaaaaa
     4621 tttcccggcg tgaaaggcgg tcgtgccaga cttattctgg tcgatacgca agtttgcgag
     4681 tttattcaga acacgccagc cttccataac acaccaatgt tgatggaagc tgaagagaga
     4741 attgctgaat acgctcctgg agcacgcgcc ccggcgtatc gacaaatcat caacgcgata
     4801 gataatatga cagacattga gcaggaaaaa gtcgcgcaat ttttgtcacg tgaaggaatt
     4861 cgcaatttcc tcgcccgtct cgatattgac gaatcagcat aaataaaaaa cggcaggata
     4921 ttatctcctg ccgtttatct ttttacacgc taattactgc tgattttcgc gttcagcaat
     4981 aaaatccagc gctttgttga tacgctcgat actgcgggtc ttaccaattg cgtgaacggt
     5041 aacatccagt gctggagact gccccgcacc ggttacggcg acacgcagcg gcataccaac
     5101 tttacccata cccacttcca gctcatccgc cgtcgcctga atagcgtgat gaacgttttc
     5161 agcggtccag tcagtaatcg cggccagttt gtcacgaacc acttccagcg gctgacgcgc
     5221 taccggacgc agatgttttt tcgccgcatc ggcatcgaac tcagcaaaat cttcgtagaa
     5281 ataacggcag ctctgtgcca tctctttcag cgtcttgcag cgttcgccca gcagtttcac
     5341 cagatcagcc agctgcgggc cgttacgggt atcgatattt tcctgctcaa tgtgccactg
     5401 taagtgagta gcaacatact ccggcggcag cgcgttaatg tagtgatggt tcagccacag
     5461 cagcttgtcg gtgttgaacg cactggcaga tttgctgacg gcattcaaag tgaagtattt
     5521 gatcatctct tcacgagtga agatttcctg atcgccgtgg gaccagccca gacgcaccag
     5581 atagttcagc agtgcttctg gcaaataacc gtcatcacga tactgcatta cgctgactgc
     5641 cccgtgacgt ttggacagtt ttttaccgtc atcgccattg atcatagaaa cgtgcgcgta
     5701 aaccggcacc ggcgctttca gggccttaag aatgttgatc tggcgcggcg tgttgttgat
     5761 atggtcttcg ccacggataa cgtgggtgat ttccatatcc cagtcatcga caaccacaca
     5821 gaagttatag gttggggaac catcggtacg gcggataatc agatcatcca gctcctggtt
     5881 gctgaactcg atcggaccac ggatctgatc gtcaaaaaca acagaacctt cctgcggatt
     5941 agcaaaacgc acgacacacg gttcatcatc agcatgatgc tcatggctgt ggcggcagcg
     6001 accgtcataa cgcggcttct cacctttcgc catttgctct tcgcgcagcg cttccaggcg
     6061 ctctttagag caatagcatt tataagcagt gccctcttcc agcatctgat cgatcaccgc
     6121 attgtagcga tcaaaacgtt tggtctggta gtacggacct tcatcccact ccaggctcag
     6181 ccagttcatg ccatccataa tggcttcgat agcttccggc gtggaacgct caagatcggt
     6241 gtcttcaata cgcagcacga actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag
     6301 agcagtacgc gcgccgccaa cgtgcagata gcctgttggg cttggcgcga agcgagtttt
     6361 gattttcatg aaatggcctt acgtttagaa agatgccgac aaccggcaaa tcctggaaaa
     6421 attgagtggg cgatattcta tcactcccgc ctgattcctc aatgtagttc gggtttctac
     6481 cttacgcatt attgttttcg ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt
     6541 gcttaaattt acgacgaacg aacaatttct ttagaaaatg cgttgactca ttttgaactc
     6601 tccctataat gcgactccac acagcggggg tgattagctc agctgggaga gcacctccct
     6661 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccaactactt tatgtagtct
     6721 ccgccgtgta gcaagaaatt gagaagtggg tgattagctc agctgggaga gcacctccct
     6781 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccactttctc gccagctaaa
     6841 tttcttgtaa aaatgtgaag taccgaagtg ggtgattagc tcagctggga gagcacctcc
     6901 cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc
     6961 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
     7021 caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac tcaaagattc
     7081 gaagctgccg caggttcgag ttgagcgcag cgaaacaacg gagccgctcg cggcgacggc
     7141 ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgtaaaaa agcgccctaa
     7201 aggcgctttt ttgctatctg cgatttgcga aattgcctga tgcgcttcac ttagcagacg
     7261 actatttccg gcaattcctg tctcctcacc tactgtgtca atgcagccaa cagcttaacc
     7321 atcgcgggcg tcacctgctg tgtttcataa acaatatata aatctgcagg gatgcgctgt
     7381 ttgagcggac ggaaaatgac acctggccag ttcatttgtg cgtagctgtc cgctatcaat
     7441 gtgataccaa tacccatact gaccatggcg agtaccgttt gcggttcatt aacttcgcga
     7501 ataacaaccg gtgaaaatcc cacctgctgg caaactcgct gcaaaaaatc ccagtcagtg
     7561 tacacgggcg gcattgtaac aaaatactcg tcacgtagcg cttccagcgg aacggtggaa
     7621 aatgatgaga gatgatgctc ttcaggcatc gccaccagaa acgccgattc atgcaaccgt
     7681 aagctggtaa aacctgtcgg tggttctgtc gccattcgcc agatcccggc atcgagttca
     7741 cgtcgttcca gcagggccat ttgcatcgcg ggcatctttt cgcgaaaaag aacttcaacg
     7801 ttaggatttt ccctgaggaa tcgccgcata accgggcgca tccgtcccca catagccgtt
     7861 cccactacgc cgagttcaat ccgccctgct tctccccgac ctatttgttc aacccgagcc
     7921 aatacattat tagcattcac cagcaatcga cgcgattctt ccatcaagat tttgcccgcg
     7981 tgtgtcagta cgacgctgcg cgaatggcga ataaaaagct gcgtgccgag ttgattttcc
     8041 agctctttaa tatgaatact gagcggaggc tgagacatat ttaaacgcgc tgctgcgcgg
     8101 ccaaaatgca actcttccgc tacggcaaga aaataacgga gcaacttaag atctgttctg
     8161 tatacgcgtt ccataattaa gtgctccata taataaatac atcgtattaa attatcatgg
     8221 cataaagtat ttcaccacca gatatccgac atacataaac acaatggata atatacctgg
     8281 taatacattt ggtacaggaa taggcaagcg cacgattgta aacaaagcac caatgccaaa
     8341 accaacgccg gaagaaaata aaatatcttt cactaattaa cctttatcat aaaagcagct
     8401 ctgaagagca gagctgcgaa tccttttaat gagccaccgc tcgatgcttt atcttttcag
     8461 ggtcatgatt atatttaaac ccaaagaaaa atatcactgc gagaaaaaga gcatatcctg
     8521 caaacaccag ccagatagtt tgccagtctt ttacgccatc caccgaaaag taatctactg
     8581 ccatgccact cagaatcgag ccaacccatg cgccgacacc atttaccatg gtcataaaga
     8641 gcccctgcgc gctggcacga atgctggaat caacttcctg ttcgacaaat accgaaccag
     8701 aaatattgaa gaaatcgaat gcacagccat aaacaatcat cgacagcagc agcaaaataa
     8761 atccggttgt tgacggatcg ccataggcga agaagccaaa gcgcagcgtc caggccacca
     8821 tactcatcag catgacggct ttaatgccaa atcgctttaa aaagaatggg atagtcagta
     8881 taaagcccac ttctgccatc tgtgaaactg acagtaaaat ggagggatat ttcaccacaa
     8941 aactgtcagc aaactccggg ttacgggcga aatcatgtag gaacggatta ccaaaaacgt
     9001 tggtaatttg cagtaccgca cccagcatca tggcaaagag gaaaaagatg gccatgcgtg
     9061 gatttttaaa cagcacgaag gcatccagac ccagcttgct ggcaagcgat gtggtcgctt
     9121 ttttctccgc aaccggaatc ttcggcaaag tcagcgcata agccgacagc agcaatgacg
     9181 caccggacgc gatatacagc tgcagactac tcaattccag atgcagcagg cttactgccc
     9241 acatcgcgac aatgaacccc accgtaccaa aaacgcgaat gggcgggaaa gcggtcaccg
     9301 ggtcaagccc tgcctgggca agacaggaat aagagacgct gttcgataac gcaatagtcg
     9361 gcataaacgc catcgcattg actaacatca cccaaaacat catatccgga tcagttacgg
     9421 atgccgcata aaaaagtacg cccgcacaca ccaggtgaca cagcatgtat gcacgttctg
     9481 cgcgcagcca tttgtctgcg atgatcccca ttataccagg cataataatc gcggcgatcc
     9541 ctttggaact gtaaaccatg ccaacattag cgccggtgaa atgaagggta ttaatcatgt
     9601 aagagccgag ggtaaccagc cagctccccc agataaaata ttgcaaaaag gacattacct
     9661 ttaagcgcat cgcgatgctc ataatcgttt ccttgtcgca tcatttggcc tcacccgcga
     9721 ggccgtttct tattgttttt ctcaggcaat tttgcgcaga aagccacaaa taaggttgat
     9781 gaagttctgc ttagagagct ccgcagccgc cagcgtttgc gcatgtgaca atttcacatc
     9841 gcttaaacct tccgccatat tggtaatcgc agagaccgca acgactttaa ggtcgcaatg
     9901 gcgagctgaa ataacctcag gcaccacaga cataccaaca acatccccac caataatttg
     9961 catcatgcga atttccgccg cagtctcgaa attcggcccc ggatacgaga cgaacacgcc
    10021 ctccgtcaga gggaaaccct cttctttcgc cactttttgt aacagtgcgc ggtattccgc
    10081 atcgtaggca tttgccagtg agaagaagcg ctctccaaaa cgatcatcgt taagacccac
    10141 catcggcgta ccgggcatgg tgttgatatg atctttcaat gcgaccaggc tgcctgcccc
    10201 cacttccgga cgcagcgagc ctgccgcatt ggtgcagaac agtagctcgc agcccagcag
    10261 cttaaaggta cgaattgcgt cagtcataat ggtcattcca cgaccttcgt agaaatgtcc
    10321 gcgacctttc atacatacca ccggtacgcc ctggagatgg cccagcacta actcccccgc
    10381 atgaccatgt accgtactta ccgggaagcc cggcagcttt tcgtaggaaa ttgcgacagc
    10441 gttctcaatc tgatcggcca gcgcgccgag cccggaacct aaaataaagg ccactcgtgg
    10501 cgtgaaatca ggtttataag tcttgataat atctatgcaa aacagcgggt tatgagaaaa
    10561 ttgaacctga gacatacata tcctttttct gtagggtgga atctaacgcc tttttatagc
    10621 gaggttgtcc cggctcttac caatacactt ttctcacgca ttcaatagcg attcgatatc
    10681 gatggattta tatcttttct ctattaatcc acggtaaaaa ctgtattggc gatattaatt
    10741 cctttgcatt aacaatatgt cgtttttagc aaaccacttt attaagcaat ctactaataa
    10801 cgatcggcat agggaattat gctatgaaaa aacatctttt aactctgaca ctttcctcta
    10861 tattagcgat acctgttgta tctcatgcac aatttaaagg cggttttgca gatatcggcg
    10921 tgcattatct ggactggacc agccggacta ccgaaaaatc gtcgaccaaa tcacacaaag
    10981 atgattttgg ttatcttgaa tttgaaggtg gcgctaactt tagttgggga gaaatgtacg
    11041 ggttct
//
LOCUS       DAFAVF010000137        11044 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFAVF010000137 DAFAVF010000000
VERSION     DAFAVF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.137"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(203..2470)
                     /gene="adiA"
                     /locus_tag="LGK42_003508"
     CDS             complement(203..2470)
                     /gene="adiA"
                     /locus_tag="LGK42_003508"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBK2659716.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2669..3577)
                     /gene="melR"
                     /locus_tag="LGK42_003509"
     CDS             complement(2669..3577)
                     /gene="melR"
                     /locus_tag="LGK42_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBK2659717.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3860..5215
                     /gene="melA"
                     /locus_tag="LGK42_003510"
     CDS             3860..5215
                     /gene="melA"
                     /locus_tag="LGK42_003510"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK2659718.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5318..6739
                     /gene="melB"
                     /locus_tag="LGK42_003511"
     CDS             5318..6739
                     /gene="melB"
                     /locus_tag="LGK42_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBK2659719.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6878..7507)
                     /locus_tag="LGK42_003512"
     CDS             complement(6878..7507)
                     /locus_tag="LGK42_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBK2659720.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7630..9276)
                     /gene="fumB"
                     /locus_tag="LGK42_003513"
     CDS             complement(7630..9276)
                     /gene="fumB"
                     /locus_tag="LGK42_003513"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBK2659721.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9354..10694)
                     /gene="dcuB"
                     /locus_tag="LGK42_003514"
     CDS             complement(9354..10694)
                     /gene="dcuB"
                     /locus_tag="LGK42_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBK2659722.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2596 a         2686 c         2915 g         2847 t
ORIGIN      
        1 tacaaatttt tatagtaatt tctcatgtac atattttttt acaggataaa taacaggcaa
       61 aaaaaagcgc ggggtggggc cgcgcaaaaa gtatgacatt tttgtttgca gcaattttta
      121 tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg cgtaatgtta tttaaacaat
      181 tacgccttca gcggaatagt ggttacgctt tcacgcacat aacgtggtaa ataccgtcaa
      241 taatttcagt cccttcagtt tcgtgttcaa atccagggaa atggtggtcc caggattgca
      301 gcgagcgtaa ataacttact tgcggactgt ttttatcgcc gaagttttca ccagacagca
      361 gcatcgggat tcctggcgga tacgggataa ctgagtttgc cgcgatgcgt cctggcagat
      421 tttcaatgga taccagttcg acattgttgt cgacaatcgc gttgtacgct tcacgcgggg
      481 tgatttccgc catcggcagg ccggaatagg cttcgttcaa ccgtgcgcca gggttgtttt
      541 ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat ccccatgttc gcgtaagtgt
      601 caggatattg ttcaacaagt tccggcatca cctgcgccag cggtgtgttg gcgtcatagt
      661 ggcgtttgaa ggagcaaagg gtgttaacca gagtccccca tttcccacgg gttacgccca
      721 tagagaacag gaacataatt tggaagtcag tggtgcgggt aggcacaatg ccgtggcgac
      781 caagccaggc agtgaccagc gccgccggaa caccggtttc ttccagttca ccatcttcac
      841 ccattcccgg agcaaggatg ctgactttaa tcgggtcgag catactccag ttatccggaa
      901 tatctttgaa gccgtgccag ctttcgcccg gatgcattac ccagcagtcc tgaacggtgg
      961 tcagcagttt ggttggtgcg tcagcaaagt cataggtttt gccggtttgt gggtcggtga
     1021 cgacttcttt gttccacggt ttgaagaacc agctaccgtc agcggtgaac tctttataca
     1081 gccgcgccat cgcctgacgg aaatcaaccg cttcgtcgat cacttcctgc gtcagtgaca
     1141 ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt ggatgcgcag atggcataca
     1201 gcggggaggt ggtggcatgc atcatgtagg cctggttaaa gcgggagaag ttaatcgccc
     1261 cacgaccttc acgtacatga atataagaag cctgtgaaag cgcattcagc agtttgtggg
     1321 tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc aggttcgccg cgcatggcat
     1381 agtgatcggc atagatcggg ttgaaacgtg catagccgta ccaggcttcg tcaaagtgca
     1441 gacgatcgga ggttttttcc agcagatcct gcgcttcttt agcgttataa cacacgccgt
     1501 cataggtgca gttggtcacc acgcagtaag acggtttttg cccagctttg tctttagtca
     1561 gcgggctttc actgattttc ttctgcaagg tttcaggttg catttcctgc ggatagattg
     1621 gcccgataat gccgtagcgg ttgcggcttg gcaccatgta gactggtttc gcgcctgtca
     1681 gcatcaagcc ttgttcgatg gatttatggc aattacggtc aacgaccacg acatcgttat
     1741 cggtcatgca agcctgcatg atggtgcggt tagagccgga agtaccgacg actaccgacc
     1801 aggagcgatc ggcaccaaat acgcgtgcgg catatttttc gctttcgcca aatgcgccag
     1861 tatggtcaag caaagaaccg agggaagttc gttcgatgcc catatcggtg cggaacagat
     1921 tttctccata gtagtcatgg tagaaacgtc cggcgggtgt tttggtaaaa ccaacgccgc
     1981 cctggtggcc tggcgctgcc caggaatatt catggatgtc gctatatttc atcagcgcgc
     2041 tgaacagtgg cggcaacagc tgctggcggt agcgggtcat cgcggcaaca gcgcgtccgg
     2101 cgataaagtc ggcggtatct tccagaatcc aggcgaattc atcgacaagc tccagcaggt
     2161 cgcgatccat tgcggcgagg gctttttccc gatcgcccaa caggaagacc ggcacgtttt
     2221 gttggcgctc atgaagctta ccgatcaatt gtctgacgtt ttgatgttcg tccggatgtt
     2281 ccatttgata actgaacatc aggcagtcaa tggcttcgtt tgaagagaga atggcaaaac
     2341 catcatcaaa ggaggtggat ttaatcacgg taacattttg ctggcttaaa gcatctgcca
     2401 gacgctcaac ggcgttaccg acccaggtgt cttgatggag aaactcgctt tcaacaatta
     2461 atactttcat cattgcttac ccggttatga aggaatcttc gttgcgggca agtatcttcc
     2521 ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt ttgaaatatt tatttttata
     2581 aacaatgcgt tatgtgatta ttttaaatta ttttcactgc ttattatcga cggctaaact
     2641 atttttttgg ctgatactga tatcgtcttt agccgggaaa cgtctggcgg cgctgttggc
     2701 taagtttgcg gtattgttgc ggcgacatgc cgacatattt gccgaacgtg ctgtaaaaac
     2761 gactacttga acgaaagcct gccgtcaggg caatatcgag aatactttta tcggtatcgc
     2821 tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat gtactgtttc atcgtcaatt
     2881 gcatgacccg ctggaatatc cccattgcat agttggcgtt aagtttgacg tgctcagcca
     2941 catcgttgat ggtcagcgcc tgatcatagt tttcggcaat aaagcccagc atctggctaa
     3001 cataaaattg cgcatggcgc gagacgctgt ttttgtgtgt gcgcgaggtt ttattgacca
     3061 gaatcggttc ccagccagag aggctaaatc gcttgagcat caggccaatt tcatcaatgg
     3121 cgagctggcg aatttgctcg ttcggactgt ttaattcctg ctgccagcgg cgcacttcaa
     3181 acgggctaag ttgctgtgtg gccagtgatt tgatcaccat gccgtgagtg acgtggttaa
     3241 tcaggtcttt atccagcggc caggagagaa acagatgcat cggcagatta aaaatcgcca
     3301 tgctctgaca ggttccggta tctgttagtt ggtgcggtgt acaggcccag aacagcgtga
     3361 tatgaccctg attgatattc actttttcat tgttgatcag gtattccaca tcgccatcga
     3421 aaggcacatt cacttcgacc tgaccatgcc agtggctggt gggcatgata tgcggtgcgc
     3481 gaaactcaat ctccatccgc tggtattccg aatacagcga cagcgggctg cgggtctgtt
     3541 tttcgtcgct gctgcacata aacgtatctg tattcatgga tggctctctt tcctggaata
     3601 tcagaattat ggcaggagtg agggaggatg actgcgagtg ggagcacggt tttcaccctc
     3661 ttcccagaga ggcgagggga gcgtccgagc atcatgaggc cgaaaactct gcttttcagg
     3721 taacttatta ccataaactc agatttactg ctgcttcacg caggatctga gtttatggga
     3781 atgctcaacc tggaagccgg aggttttctg cagattcgcc tgccatgatg aagttattca
     3841 agcaagccag gagatctgca tgatgactgc acccaaaatt acatttatcg gcgctggttc
     3901 gacgattttc gttaaaaata ttcttggtga tgtgttccat cgcgaggcgc tgaaaacggc
     3961 gcatattgcc ctgatggaca ttgatcctac ccgcctggaa gagtcgcata ttgtggtgcg
     4021 taagcttatg gattcagcag gggccagcgg caaaatcacc tgccacaccc aacagaaaga
     4081 agccttacag gatgccgatt ttgtcgtggt ggcatttcag attggcggtt atgaaccttg
     4141 cacggtgact gatttcgagg tctgtaagcg gcatggtctg gaacaaacca ttgccgatac
     4201 gttggggccg ggcggtatta tgcgcgcgct acgtaccatt ccgcatctgt ggcaaatttg
     4261 cgaggacatg acggaagtct gccccgatgc caccatgctc aactatgtta acccaatggc
     4321 gatgaatacc tgggcgatgt atgcccgcta tccgcatatc aaacaggtcg ggctgtgcca
     4381 ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc aatatcgacc cagctacgct
     4441 gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac ctggagctgg agcgcaaaac
     4501 cgccgacggc agttatgtga atctctaccc ggaactgctg gcggcttatg aagcagggca
     4561 ggcaccgaag ccgaatattc atggcaatac tcgctgccag aatattgtgc gctacgaaat
     4621 gttcaaaaag ctgggctact tcgtcacgga atcgtcagaa cattttgctg agtacacacc
     4681 gtggtttatt aagccaggtc gtgaggattt gattgagcgt tataaagtac cgctggatga
     4741 gtacccgaaa cgctgcgtcg agcagctggc gaactggcat aaagagctgg aggagtataa
     4801 aaacgcctcc cggattgata ttaaaccgtc acgggaatat gccagcacaa tcatgaacgc
     4861 tatctggact ggcgagccga gtgtgattta cggcaacgtc cgtaacgatg gtttgattga
     4921 taacctgcca caaggatgtt gcgtggaagt agcctgtctg gttgatgcta atggcattca
     4981 gccgaccaaa gtcggtacgc taccttcgca tctggccgcc ctgatgcaaa ccaacatcaa
     5041 cgtacagacg ctgctgaccg aagctattct tacggaaaat cgcgaccgtg tttaccacgc
     5101 cgcgatgatg gacccgcata ctgctgccgt gctgggcatt gatgaaatat atgctcttgt
     5161 tgacgacctg attgccgccc acggcgactg gctgccaggc tggttgcacc gttaaaacgc
     5221 gactaaacgc tactgcgccg ggggatttat tccggcgcac acctctgacg ataccaataa
     5281 cagaaggcgg gcgttggtaa cagcgacccg ataccctatg agcatttcaa tgactacaaa
     5341 actcagttat ggatttggag cgttcgggaa ggattttgcg atcggcattg tgtatatgta
     5401 cctcatgtat tactacaccg atgtcgttgg gctgtctgtg ggtttggtcg gtactttgtt
     5461 tctggtggcg aggatctggg atgctattaa cgatccgatt atgggatgga ttgtaaatgc
     5521 tacgcgatcg cgatggggta agttcaaacc ctggatcctg atcggtacgt tggcaaactc
     5581 tgtaatctta tttctcctct ttagtgcgca tctgtttgaa ggtactactc agattgtctt
     5641 tgtttgcgtg acctacatcc tctggggcat gacttacacc attatggata ttcccttctg
     5701 gtcgctggtt ccaaccatca cgctcgataa acgtgagcgc gaacaactgg ttccttatcc
     5761 gcgttttttt gccagtctgg caggctttgt tacggcaggt gtgacgctac catttgttaa
     5821 ttatgtcggc ggtggcgatc ggggatttgg ctttcagatg ttcactctgg tactaatcgc
     5881 cttttttatt gtttcaacca tcatcactct gcgcaatgtg catgaagtct tttcgtcaga
     5941 caatcaaccg tctgctgaag gaagccatct gacacttaaa gccatcgttg cgctaattta
     6001 taaaaacgat cagctttcat gcctcttggg tatggctctt gcttataatg tagccagcaa
     6061 cattattacc ggatttgcta tctattattt ctcatatgtt atcggtgatg cggatttgtt
     6121 cccctattat ctgtcgtatg cgggagctgc taacctggtg acgttagtat tcttcccacg
     6181 cttagttaaa tcattatccc gacgcatttt atgggccgga gcatctattc ttccggtgtt
     6241 aagctgtggt gttctcctgt taatggcatt aatgagctat cacaacgtcg tcctcattgt
     6301 gattgcgggt attttgctga atgtgggaac ggcgcttttc tgggtattac aggtcatcat
     6361 ggtggcagat accgttgatt acggtgaata taaactgcac gtacgctgtg aaagtatcgc
     6421 ttactccgtg cagactatgg tggtgaaggg cggttcagcc tttgcggctt ttttcattgc
     6481 ggttgtgtta gggatgattg gctatgtacc gaatgttgaa cagtctacgc aagccctatt
     6541 aggtatgcag tttattatga ttgctctacc aactctgttt ttcatggtaa cgctgattct
     6601 ctacttccgt ttctatcgcc tcaatggcga cacgctgcgc aggatccaga ttcatctgct
     6661 ggataaatat cgcaaagtac cgcccgagcc tgttcatgct gatattccgg tcggtgcagt
     6721 gagtgatgtg aaagcctgac gtggacaata gctaacgaaa atggcctgat gcgatatgtt
     6781 tatcaggcca cgtcttaatg taatgctttg gatttgcaaa ttttgtagac cggataagga
     6841 attcacgccg catccggcat caacaaagcg cacgttgtta tccggttatc aagccaaagc
     6901 gccgtagctg gcggcaatga aagcgagcga ggaatatcac cgtcgttaat gcgccaagca
     6961 gcgcgcagaa catatcggat tgcgtgtccc actggtcgcc ctgggtgccg agaaaatcat
     7021 ccgctccctg gcccattgcc agcgccgccc accactcgat taattcatac atcgcgctta
     7081 tcgccagcgc cacgcagcag accaggaacg ccaccatttt acgtccgcgc acgtacatcc
     7141 cgcgaacgag aatttctcgt gccaccagtg caggcaccag cccctggaaa aaatgcccca
     7201 gcttgtcata cggattacgg ctcaacccta accattcctg cacctcaaaa ccaacgggga
     7261 ctttcgcgta ggtgtattgt ccgccgacca tcaggatgat ggcgtgaaag aaaatgagcg
     7321 tatagagcag cggcgttaac ggataacgtt tcgcggtggc aaggagtagc ggcacgacaa
     7381 taatcaccgg tgtcacttcc atcagccagg tgagtttgtc atgggccgaa atgccggtat
     7441 aaatcaggat taacgttagc gccagtgcgc cggtgttaag aattaacggc ttgagtgtgc
     7501 gggtcatggt gagttcgcaa gtcagggaaa atactgacta ttcaccagca agcgtgaaat
     7561 tacaatcgca aaagaggtgg cccaggggga tcacctggca gcatgctgcc aggcgctggg
     7621 ccgaagaggt tacttagtgc agttcgcgca ctgtttgttg acgatctgct ggaagaagtc
     7681 gttacctttg tcatcgacca ggataaacgc cgggaaatct tctacttcga ttttccagat
     7741 agcttccata cccagctccg gataagcgac gcattccaga tgcttgatgc tctgctgcgc
     7801 cagtaccgcc gccggaccgc cgatgctacc gaggtagaag ccgccgtgtt tatgacacgc
     7861 gtcggtaacc tgctgactgc ggttaccttt cgccagcatg atcatgctgc cgccgtggga
     7921 ttgcagcaga tccacgtagg agtccatacg gcctgcggtg gttgggccaa gtgaacctga
     7981 tggataaccg gcaggggttt tcgccggacc cgcgtagtag atcgggtgat ctttgatgta
     8041 ctgcggaagt tctttaccgg cgtcaatcag ctctttcagc ttggcgtgtg caatatctcg
     8101 gcccacgata atggtgccgg tgagcgacaa acgagtggat accgggtatt gcgaaagctg
     8161 ggcgaggatc tctttcatcg ggcggttaag gtcaactttc accgcttcgc cttcaccggc
     8221 ctggcgcagt tcttgtggaa tgtactggcc tgggttgtgt tccagttttt cgatccagat
     8281 accttcgcgg ttgattttcg ctttaatgtt acggtcagcg gagcaggaga cgcccatgcc
     8341 gaccgggcag gatgcgccgt gacgtggcag acggataacg cgaatgtcgt gcgcgaagta
     8401 tttaccgcca aactgcgcgc caagaccgag tttctgggcc tcttccagca gttcctgttc
     8461 cagctggaca tcgcggaacg cctgaccatg ttcgttccct tccgtcggca gttcatcgta
     8521 atagtgagcg cttgctaact tgacggtttt caggttggtt tccgcagacg taccgccaat
     8581 cacaaacgcg atatggtacg gcgggcaggc tgcagtaccg agggtacgca ttttctcgac
     8641 gaggaagttt ttcagtttgc cgggagtcag cagggctttg gtttcctggt agagatacgt
     8701 tttgttggca gagccgccgc ctttcgcaac gcaaaggaat ttgtactcat cgccatctac
     8761 cgcgtacagg tcgatttgcg caggcaggtt agtgccggtg ttgacctctt tgtacatgtc
     8821 cagcgccgca ttctgtgaat agcgcaggtt atcttcgata taggtgttat agacgccttt
     8881 cgacagcgct tcttcatcac cgccgccggt ccacacgcgc tggccttttt taccgacgat
     8941 gatcgcggtg ccggtatcct ggcaggtcgg cagcacgcct ttggcggcga tttcggagtt
     9001 tcttaagaat tgcagcgcca cgtacttgtc gttttcgctg gcttctggat cgtgaagaat
     9061 agccgcaacc tgtttctggt gtgccgggcg gagcataaaa gaagcgtcgt gaaaggcttg
     9121 ctgcgccagc agggtcaggg cttctggttc cactttcagg atggtttcgc cgtcgaagtc
     9181 ggcaacgcta acgtaatcgg aagtgagtag atagtattcg gtattgtctt tccccatcgg
     9241 gaaaggtgcc tggtagataa agggtttgtt tgacatagct tccagcctgt aactctgtat
     9301 ttgttattcg aaaatggcgt gccgcgtagt cacgggcggc acgcaaagtg catttataag
     9361 aacccgtaca tcgcggcgaa gatccagccg aagacgcacg atacgctcac accaatcaac
     9421 cccggcagaa taaagctgtg gttgatgacg aagcgaccga tgtgggtggt gccggaacgg
     9481 tcaaactgaa tcgctgccag atcgctcgga taagtcggca ggatgtaata accgtagcaa
     9541 gccggtgctg aagccacgat gtatgccgga tcaacgccga tcgccagcgc gaccggaaca
     9601 atcgccgcca gcgccgcagc ctgagagttt acaaacttgg aaaccagcag cagaacaatg
     9661 gcataggccc acggatactc tttcaccatt tcacccagta cgccctgaat ttcagacata
     9721 tgcgcaccga acatggtttc tgccatccat gcgataccgt acaccgccac gatggcgatc
     9781 ataccggaac ggaagacttc gttttttgag atagacgcgg gattggtttt ggtcaggata
     9841 ataatcagcg ccccggtcag cagcataaac atctgaataa ccagtaccat cgacagcggt
     9901 ttgccgccga aggatggacg caggtccgaa tcagcaccaa gaagggcgac tacagcgatt
     9961 gcaccgagga aaatccacat tgccagccag ttgcttttcg gcagtttttt atcgagcagc
    10021 gtcgcggtat caccgtaaac atactcacgg ttttccggta cggagatgaa tttctggaac
    10081 tcttcgtctt tatccagatc tttaccgcgg aaccagctga agataccgat cgccaggata
    10141 ccgattaacg tcgatggaat ggtgattgcc agcaggtcga ggaactcaag atggcgacca
    10201 tcaaaggtga cattacccag cattgcaacc agagacacga ccgcaaccga caccggactg
    10261 gcgataatcc ccatctgtgc accgatagaa cttgccgcca tcggacgttc cggacggatg
    10321 ttgttcttaa tggcgacgtc gtagatgatc ggcagaatgg tgtaaaccac atgacccgta
    10381 ccgcaaagaa tggtcagtgt acaggtcaca aacggcgcga caattgagac atatttcggg
    10441 ttgcggcgca gcaacttctc ggcaatttgc agcatgacat caagaccgcc cgaagcttgc
    10501 aaggtcgccg atgccgccac caccgcaatg ataaccagca tgacatcaac tggtggttta
    10561 cctggctgaa ggtggaagac gaagaccaga atgaccagac cgataccgcc taataaaccc
    10621 agcgcgatac caccctttct ggcaccataa aacagacata tcagtattat gataagttgg
    10681 atagtaaata acatgtgtga accctcgcga taatcctatt taaatttttg ctgaatagat
    10741 cacagtcacg ttctgttttg tatgaactgt tttcagagcc tgactaaata tccgtttggt
    10801 cgttaccggc tttagcaaat acctcacagt aaatattggc tgattaaagc agcggcaaat
    10861 ttatgcactg tgataagcgg ctttttcaga gagaaaaact ctctggcgat gggtttatag
    10921 tttgtactgc gcagatgtta atgttttgtt tgctgttatc ttattgatat ttatcggtct
    10981 attttttgtt ctcagggcag atatcattag gtttaaagat taatctgatc tacccatttg
    11041 tggg
//
LOCUS       DAFAVF010000138        10970 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFAVF010000138 DAFAVF010000000
VERSION     DAFAVF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.138"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            53..814
                     /gene="adiY"
                     /locus_tag="LGK42_003515"
     CDS             53..814
                     /gene="adiY"
                     /locus_tag="LGK42_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBK2659723.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            951..2288
                     /gene="adiC"
                     /locus_tag="LGK42_003516"
     CDS             951..2288
                     /gene="adiC"
                     /locus_tag="LGK42_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBK2659724.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2392..4035
                     /gene="eptA"
                     /locus_tag="LGK42_003517"
     CDS             2392..4035
                     /gene="eptA"
                     /locus_tag="LGK42_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBK2659725.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4032..4700
                     /gene="basR"
                     /locus_tag="LGK42_003518"
     CDS             4032..4700
                     /gene="basR"
                     /locus_tag="LGK42_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBK2659726.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4701..5801
                     /gene="pmrB"
                     /locus_tag="LGK42_003519"
     CDS             4701..5801
                     /gene="pmrB"
                     /locus_tag="LGK42_003519"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBK2659727.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5777..5866)
                     /gene="pmrR"
                     /locus_tag="LGK42_003520"
     CDS             complement(5777..5866)
                     /gene="pmrR"
                     /locus_tag="LGK42_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBK2659728.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5978..7480)
                     /gene="proP"
                     /locus_tag="LGK42_003521"
     CDS             complement(5978..7480)
                     /gene="proP"
                     /locus_tag="LGK42_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBK2659729.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7744..8622)
                     /locus_tag="LGK42_003522"
     CDS             complement(7744..8622)
                     /locus_tag="LGK42_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBK2659730.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8619..10847)
                     /gene="crfC"
                     /locus_tag="LGK42_003523"
     CDS             complement(8619..10847)
                     /gene="crfC"
                     /locus_tag="LGK42_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBK2659731.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2743 a         2855 c         2699 g         2673 t
ORIGIN      
        1 tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat gtatgaggat
       61 ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga taagggtgaa
      121 tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt gtgaaaataa
      181 tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc acgacatcat
      241 caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat ggcaacgtag
      301 cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg ttgccgcgca
      361 acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg cattattatt
      421 cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga tgtatatgtt
      481 acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc acaaagactg
      541 gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa agaaaaagtt
      601 gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc gttatgccgt
      661 aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct gcggctacaa
      721 cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc cgctgcatta
      781 tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga agagctatat
      841 taataacggc atcagcgata acccggtcga taataattca actatcgaat gcaggcgtat
      901 gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt atgtcttcgg
      961 atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg gggaatatta
     1021 tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg attgccattt
     1081 atggatggtt ggtgacgatt atcggtgcac tggggctctc gatggtatac gccaaaatgt
     1141 cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc tttggcccgt
     1201 ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc aatatcgcca
     1261 tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa gatccattgg
     1321 tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac attgtcggtc
     1381 cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc cccatcgtcg
     1441 ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg gcatggaacg
     1501 tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg ctgtggtcgt
     1561 tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg aaacgcaatg
     1621 tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta ctttctacca
     1681 ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg ccattcggtg
     1741 atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc tgcgcagctg
     1801 cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg gcgaaagccg
     1861 ctgccgatga cggactgttc ccaccaattt ttgcccgtgt aaataaagcg ggtacgccag
     1921 tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc agcatttcac
     1981 caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt acactggtgc
     2041 catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt ggtaaagcac
     2101 gcccggcata tctggcagtt actaccattg ccttcctcta ctgcatctgg gccgtggtgg
     2161 ggtccggagc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc accgccatgt
     2221 atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca ccaataagca
     2281 aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact taaggttggc
     2341 ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt gatgttgaag
     2401 cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc cgctttttat
     2461 atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct gccgctggat
     2521 tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag cgtgattaat
     2581 attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg cctgtttatt
     2641 ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat cgaccgctcg
     2701 atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat gacaccgcaa
     2761 atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg ctggataaaa
     2821 atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa tattctggtt
     2881 tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc ctcgttgttc
     2941 cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt tgccagctgg
     3001 tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg tgaagacgcc
     3061 caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct gattgtcggc
     3121 gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac taacccgcgg
     3181 ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac ggcaacggca
     3241 gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga agagctggca
     3301 cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt gctgtggaat
     3361 gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa cgtcaccgcg
     3421 ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtact gttccacggg
     3481 ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca caccatcggc
     3541 agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt taccccaacc
     3601 tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac ttacgacaac
     3661 acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa agaacatcag
     3721 gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt aggtgaaaat
     3781 ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa acaggtgccg
     3841 atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca gaactgcctg
     3901 caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac gctattggga
     3961 ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca aacttgcagg
     4021 agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg cagggattga
     4081 ttctggcggc gcaaactgaa ggctacgcgt gcgatggcgt gacaaccgcg cggatggcgg
     4141 aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg ttacccgacg
     4201 aagatggact gcattttctc gcccgtatcc ggcagaaaaa atataccctg ccggtactga
     4261 tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc ggtgccgatg
     4321 actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc gccctgctac
     4381 gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg ctgaatatgg
     4441 gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa gaatatgctc
     4501 tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt ctctacaacg
     4561 acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat atccacaatc
     4621 tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc tatatgctgg
     4681 tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca atatcgctgc
     4741 gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg atcagcgtct
     4801 tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg ctacgcgaca
     4861 atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc agcctgattg
     4921 tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg gtacgccgca
     4981 tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc gacaacttaa
     5041 cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg gcgttgaacg
     5101 atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc gctgacgtcg
     5161 cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg ctggcgaaaa
     5221 cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg gagagcgtct
     5281 cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat tatcaacatg
     5341 taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc atgctcgacc
     5401 agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt cagggtgatg
     5461 caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt tacagcccgc
     5521 aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg gcagttgaag
     5581 atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg tttgtacgta
     5641 tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc attacacagt
     5701 tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg cgggcctggg
     5761 tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg ctgaccacca
     5821 gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca ctctcctccg
     5881 ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt tcaattggcc
     5941 tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt cgcggatgtt
     6001 gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca atattatcga
     6061 ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc tgtatatctg
     6121 acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg gttacgccgg
     6181 tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc agattctgcg
     6241 agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc accgaaatat
     6301 taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct ggcaaggtag
     6361 aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac ccggcaaaaa
     6421 tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac acaaacaggg
     6481 caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc aagcccatca
     6541 ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc ccgtggtctt
     6601 cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac atgtagtaag
     6661 tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag tgtttggtgg
     6721 caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg ccctgttcca
     6781 gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc aggtaaagcc
     6841 cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag tcgaggaagt
     6901 tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc acaaacccgg
     6961 caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca ggtgagtatt
     7021 ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct tgtgccatct
     7081 tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac ggtattaagc
     7141 caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc ttctggcgac
     7201 catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc agaaagggaa
     7261 cggagaacgt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc gggaaaaaaa
     7321 ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat tccattgcat
     7381 tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata atggtgacat
     7441 cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc gcagagttgg
     7501 cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga gcgggaattg
     7561 caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg ttaaggaatg
     7621 tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac cctaccagtg
     7681 tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga tgtaactgat
     7741 tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccgcca gacgctgcgc
     7801 tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca ctgaccagca
     7861 aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat cagcatgatc
     7921 cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac gcgtggtcgg
     7981 ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat cacccagcaa
     8041 caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc cccaacgcaa
     8101 ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg ccccagcacg
     8161 ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc ctcgttgcgt
     8221 taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg cggcaatttg
     8281 ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca gcagcgccag
     8341 atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg ccagtttttc
     8401 caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg tccatgtctg
     8461 aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct gctgatgggc
     8521 ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg gataaaccga
     8581 ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc tgccgtgaaa
     8641 agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc gtgagtcatc
     8701 acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg ttgacgcaga
     8761 gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa ctcagcgaaa
     8821 aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac tttagccatt
     8881 tgttggcaat aaagatcgac ttctgcgctt aaagctcgtt gcaacacact gtaatcaacc
     8941 gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa ccagcgcgaa
     9001 aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcatc ctggcgcgaa
     9061 atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg aaaaacagga
     9121 aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg ttgctcaatg
     9181 ggccgaataa catcggttaa cacccggcaa agggtggatt caagctcggc aaaacgcagc
     9241 gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca gcaggtacga
     9301 atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc attcagcgca
     9361 tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat ttcatgttta
     9421 acctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc ttcgacctga
     9481 tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg aaaatccagg
     9541 tatccccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg cagagcatac
     9601 aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc gaacagcgaa
     9661 tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag atcgtcatga
     9721 cgccagcgcc tgccgagcgc agcatgggca aaatcttcca cccagcgttg ttgctctggc
     9781 gctggtaact taccgttgtt ggctaactca tggcgcgctc gattcgccag gtagccccac
     9841 atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag cgtcccggaa
     9901 atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc gaacttattg
     9961 accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg gacctcttca
    10021 tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga ggcgcgtgcc
    10081 agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg cccgggggta
    10141 tccagtaacg tcaactgacc gggataactc tccagccccg ccagatggac aaactccact
    10201 tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc gacgtccagc
    10261 gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat aggctgggca
    10321 cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag cgcacgcata
    10381 tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg caggcgctgt
    10441 tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag taccggttcc
    10501 ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg attacgatta
    10561 ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc tttcatggta
    10621 ccgacaattg ccagcaccat ttccagtcgg gaaattttac gcaactcatt attcagcatc
    10681 gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg cgcgtcgtct
    10741 ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga aagctgtaac
    10801 aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga aaattcttcc
    10861 ttaaagcaaa ttttgttact tttatttagc cagattgttt ttgagttctg ttttcggctt
    10921 ttataattac tgcaagaaat aattttatat ttagtgtgtt gttttttatc
//
LOCUS       DAFAVF010000139        10918 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFAVF010000139 DAFAVF010000000
VERSION     DAFAVF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.139"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..156)
                     /locus_tag="LGK42_003524"
     CDS             complement(<1..156)
                     /locus_tag="LGK42_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBK2659732.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTI"
     gene            400..657
                     /locus_tag="LGK42_003525"
     CDS             400..657
                     /locus_tag="LGK42_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBK2659733.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(707..1657)
                     /gene="uspE"
                     /locus_tag="LGK42_003526"
     CDS             complement(707..1657)
                     /gene="uspE"
                     /locus_tag="LGK42_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBK2659734.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1809..2561)
                     /gene="fnr"
                     /locus_tag="LGK42_003527"
     CDS             complement(1809..2561)
                     /gene="fnr"
                     /locus_tag="LGK42_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBK2659735.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2756..3271)
                     /gene="ogt"
                     /locus_tag="LGK42_003528"
     CDS             complement(2756..3271)
                     /gene="ogt"
                     /locus_tag="LGK42_003528"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK2659736.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3282..4808)
                     /gene="abgT"
                     /locus_tag="LGK42_003529"
     CDS             complement(3282..4808)
                     /gene="abgT"
                     /locus_tag="LGK42_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HBK2659737.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(4845..6290)
                     /gene="abgB"
                     /locus_tag="LGK42_003530"
     CDS             complement(4845..6290)
                     /gene="abgB"
                     /locus_tag="LGK42_003530"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBK2659738.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(6290..7600)
                     /gene="abgA"
                     /locus_tag="LGK42_003531"
     CDS             complement(6290..7600)
                     /gene="abgA"
                     /locus_tag="LGK42_003531"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBK2659739.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            7776..8684
                     /locus_tag="LGK42_003532"
     CDS             7776..8684
                     /locus_tag="LGK42_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659740.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9014..9577
                     /gene="smrA"
                     /locus_tag="LGK42_003533"
     CDS             9014..9577
                     /gene="smrA"
                     /locus_tag="LGK42_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBK2659741.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(9598..10830)
                     /gene="dgcM"
                     /locus_tag="LGK42_003534"
     CDS             complement(9598..10830)
                     /gene="dgcM"
                     /locus_tag="LGK42_003534"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBK2659742.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         2798 a         2700 c         2701 g         2719 t
ORIGIN      
        1 tatggtgtag attaaaaatt gcaccgcatg gaataaaaaa ccttttcttt tacgatttcc
       61 gcggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
      121 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
      181 attgtcgtca tgccggaaac ccctggcggg gctattttac cgcgacaatt cattcagatc
      241 atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa
      301 tccagctaag aggtgaggtt ttcagagcag acaacggtga aatgtcatgg tattgttacg
      361 tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag
      421 tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg
      481 gttgtgactc gcgcgactat cggcggcgtt atagaacagt acaatattcc gctgtctgag
      541 tggacgacat caatgtatgt gattcagtca tcgatgattt ttgtttatag cctggtcttt
      601 actgtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta
      661 aaaaataggc ccgataactc gggccttgtc agttattgaa gagtcgttaa tcgtcttctt
      721 cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccaac agatcgcagc
      781 gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc
      841 gtcctaccgt gcccagaacc acaatccccg cctgtaagtg ttccgccaaa tcaggaatca
      901 cctcttctgg cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc
      961 gcagagcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcggat
     1021 caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat
     1081 gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat
     1141 tgagcgcatt atgatacggc tcttcactgg cgagattcac cgccaccagc gccttgcctc
     1201 cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacagat
     1261 gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta
     1321 gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat
     1381 gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt
     1441 ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agccgtacgt tcgtccgggg
     1501 agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc
     1561 caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag
     1621 gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg
     1681 tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc
     1741 ttctgccaga tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg
     1801 cggaaaaatc aggcaacatt acgcgtatga ccagcaagct gggccagcgc atcgttattt
     1861 tcgatggtga tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc
     1921 agacggctga tggtttctac cgtcaggccc agatagttac cgatatcgcc acgagtcatc
     1981 gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag
     2041 atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaggat catgtcctga
     2101 tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta
     2161 ccggacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc
     2221 tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa atcccaccag gtcgccagct
     2281 aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta
     2341 ccggagcgga tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct
     2401 ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg
     2461 agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg
     2521 ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa
     2581 ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac
     2641 agcagagaat tccgatatca gatgtaaata tatgtctatc tatttgaaaa cccttaagtt
     2701 gttaagggta actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca
     2761 gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc
     2821 cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat
     2881 tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcccaat tgttcagcca
     2941 gttggccgta atgcattacc tgcccgcagg ggatagtgcg tagagttttc cagacttcgc
     3001 gctgaaatgg cgtcccccct gtggcagtgg gaagcgtatc aataatgcta agattaccgg
     3061 caaaataatc acgaagcttg tcgcttaaac cgcctgggtt ggtggcagaa atgcgctcat
     3121 agccttcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc
     3181 attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggcg
     3241 tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa
     3301 atccccggac ctatcggcag accaacaaga taccacgcca gcaacatcag cagccatacc
     3361 accaaaaaga taagcggata gggcaagact aacgaatagt aagtacccag ttttgcgtct
     3421 ggtttgtagc gttgcaggaa tccaagaaac agtggaacaa aaggagatac cggcgctaaa
     3481 ggcaatacgg atgagtcggc aatacgaaag aggatttgcg caaatgccgg gtgaaagcca
     3541 agtagcataa acattggtac gaaaatgggg gccagaatcg accagattgc ggaaccgctg
     3601 gcaataaaca tgcataagaa agaggaaagc aacgccagac cgacaaacgc cgggatgccg
     3661 ctaagccctg aactttcaag tatatcggtc agccccacgg cgatgaattt ccccatgttg
     3721 ctccagttaa acatggcgac aaattgggcg aggggaaaaa ccatcacgat aaatcccgcc
     3781 atctctttca tcggttcaat cattaaatgc ggtaaatccg cctgacgtcg aattgtgcgg
     3841 gtagcgatgc cataagccag cgagacaaca aagaaaaaaa gaatgatcag tggcacgata
     3901 cctttaataa agggtgatgg catcacggtg tgattaatcg gatcgcgcaa tatcccgttt
     3961 tccgggatca ccatcagcgc gatcgcagca ataaaaagta gcgatacgac acctgctatg
     4021 cgtaaaccaa aacgctgact ttcggtcaat gtctgcagtt tctcatcgct gtttccctgc
     4081 cattgaccta atcgtggctc gatgattttg tcggttatca ggccgccaac aatcgtcagt
     4141 acgactacgg agctggccat aaaataccag ttatcaatta cactgacgtg catttgcgga
     4201 ttgaacgcag ccgccgcttc cgtgctgatc cccgacagca acacgtcggt tgtgacaatc
     4261 agtaaattag ccgtaaagcc gcaacctacg cctgcaatgg cagccagtaa acctgcaact
     4321 ggatgcctgc ccaccgccag aaaaatcagc gcacccatcg gtggcatgat cactaacgcc
     4381 gcatcggaag aaatgtggct gaaaaaagca ataaacagca ccatataact ggcgtagcgg
     4441 gcattaacat gcgatgccat tttaaccatt agcgctggca gtaagccgac gcgctccgct
     4501 aggccggcac ctaaaaccag cgccaggatc gctccaagtg gagcaaaacc gctaaagttt
     4561 ttaatcacat tgggtaaaaa ccagtgtaat ccttccacac tgagcaggtt tttcaccacc
     4621 accggcgtac catcggtcgg gtttttcgca ctgacgccaa aggctgacaa aattgccgtc
     4681 gtcaccatga gtacgattat caaatagata aagagcaaaa aaggatgggg aaccttgtta
     4741 ccaattcttt cgacccagcc atagagcttc ccggattggg aggacgacgg tatggatgac
     4801 atactcatgg gcattcctcg gttgttgtgt ttgcgttgtt gttgttattt taaaggtgac
     4861 ggtgtcacgt ttttcgggat agggcagtga tacggttgcg tgtccgtaac ttgctgatgc
     4921 tcttgttggc attcttgcaa tagccctgaa tcaatgaaga gattgactgt ggttgctgcc
     4981 atagttttcg ccgccagcag cattccttta tgagcaatag atgttcgccc ctggctaacc
     5041 agttgccacg tatgtagcgg tgtaccgacg gcaaaacagg ggctgaaaca ctgggcaaca
     5101 ggcagtttcc aactgacgtc gccgacatca gtcgatgccg caagcacgtt atcggtggcg
     5161 gcatatggag cgacctcatt cgccagtacc gtttcacgat gacgtagtgc aaaaaccttg
     5221 ccgttttcgc caccggttgc ggcgatatta ttcagactgt tttgccgatc gttgggggtg
     5281 agcgtagcct gaatttgttt cgcaaaagcc agttcttcgg agttccattc cggggtacca
     5341 aaatgggata gggcatggta catggcattt tctaaggtgc gattcgggag ataactggaa
     5401 caggctttgt cgaagcggca ttcaaccgtg gtttcggtca tcaatgccgc accttcggcg
     5461 attttggcga cccgatcata aatatgctgc acgtcggtca tttcgggggc acggataaga
     5521 taaagcactt ctgcctgcgc ctggaccacg ttgggcgaga tcccgccgct atctgtgatg
     5581 gcatagtgta cgcgcgcttt ttcaataata tgttcgttga ggaagttggt gccagtggtc
     5641 atcaacgtta cggcatcaag ggcgctgcgt cccaaatgag gggaattcgc ggcatgtgct
     5701 gcgatccctt taaagcgcca tgatgcctga atgtttgcca gcgtgcgggt attgaacata
     5761 ccggcaaagg cttccgggtg ccaggtgagt gccgcatcca catcatcaaa taccccctcg
     5821 cggaccatga acgttttacc cgagccgcct tcttcgccag gacaaccata aaagcgcact
     5881 gtgccgcctt gcccatattg ttccagccat ttcttgacgg ctattgcagc ggcaaaggcg
     5941 gcggttccca gcaaattgtg tccgcaaccg tgaccatttt caccgggcgt cacggatgta
     6001 ggttgcgcgc aacctgcttg ctgacttaaa cctgccaggg cgtcatattc tcccagcagg
     6061 gcgataaccg gtttgccttg accaaacgaa gcaataaagg catttgggat attgcctacg
     6121 ttgcgggtaa cggtgaagcc tgcagattcc agcgccgaag ccagatgctc cgctgaccag
     6181 aactcttcaa aacgtgtttc tggatgatcc cagatttgat cggcaatatc ggtataacgt
     6241 tggcgatcgg cttcaatcgc atcgtcgata aaacgataga tttcctgcat cagatacctc
     6301 gcgtccaggg aaaattgagc gcggtgcgcg ccagcgtttc gacggcaata gcgagaacct
     6361 gctcgtcaaa atcgaatttt tcgttgtgat gacctgccgc cagctgtgtg ccaaacacca
     6421 cgtaggaggc ttgcccttga tgttgctgca cgcgggccat cattaatgtg gcatcttcgg
     6481 aacccgcagg cgcttcaaca cgttcaatgg cctgattgac ccccgcgacc tgagccgcct
     6541 gactttgcaa ccatgcgacc cattgcggcg aaggagaact ggcggtagct gcacccatca
     6601 gacgagtttc aacgccgaca ccatacatgg ttgctgcgcc ctgaatcgct tgttgtgcac
     6661 ggtcaaaaac atattgatta atgacgtcgc tggccccgcg tgtttccact ttcagcaacg
     6721 ccgaggcagg aacaacgtta cgaccgcttc ctgcctgcat aacgcccacg tttactctgg
     6781 aagctccttc gctgtgcggg gcgattgcat gcagtgcaag agtggcttgt gctgccgcca
     6841 acaaggcatt gtgaccgtct tctggttttg cgcctgcgtg agcggcggta ccggtgaagt
     6901 gcgcgtcaaa tttggtggtt gccataaaat tatcactgcc gcacaccacg gtgcccgcag
     6961 gtacgccagt gccaatgtgc acggcagtaa aataatcaac atcatctacg acacctgcat
     7021 cgaccatcgc ccgcgcgcca cgcgtacctt cctctgcagg ctgaaaaatc agtttgatga
     7081 cgccatgtag tccggactcg aattgtttaa gggtatgcgc cagcccaagc ccaatggcgg
     7141 tatgtccatc atgaccacag gcatgcatca ttccggcgtt acatgacgca aaaccgtcgc
     7201 ggtaggggcg atggctgaca tcctgctctt cactgagatc cagcgcgtcc atatcgacac
     7261 ggaaagccat caccggaccg gggcgaccgg tatccagggt ggcgacgata ccagtgaaac
     7321 caccttcaaa aaccgcaatc cattgtgcta gcgcaccctg ttgacgagcg cgctcgaatt
     7381 cgcgttgtag agtgagttca tcaggtagtc ccatccggct actttcatta actacttcgc
     7441 ggcccagcgc cagtgaatag ccgagctgct gcaattcttc cgcaacaagg gtggcagtgc
     7501 ggaattccac ccagccagac tctgcatagt gatgaaaatc acgtcgccag tgcgataatt
     7561 ttggggcaag cgaattaaca aattgattca aagactccat aacctttccc gtcatcagta
     7621 aaaagtgtga cccggttcac gtagcgatag tttttactta tcactaactg atttttcaca
     7681 gttttaaccg ttcataaatt accctgacac aatcatctgc attaaagtag atgccagttt
     7741 ctttggtctg ataaataacg gttatcggtg gcgtcatggc ttttcaggta aaaattcatc
     7801 aaattcgtgc ttttgttgaa gtggctcgtc agggcagcat tcgcggagcg agccgaatgt
     7861 tgaatatgtc gcaaccggca ctgagtaaat ctattcagga gctggaagaa ggattagcgg
     7921 cgcaactctt ttttcgccgt agtaaaggcg tgacgttaac tgatgccggt gaaagttttt
     7981 atcagcacgc cagtcttatt cttgaagaac tgcgcgcagc ccaagaggat attcgccaac
     8041 gacaagggca actggcaggg cagattaata tcggcatggg ggccagtatt tcccgcagtc
     8101 tgatgccagc tgtcatatct cgttttcatc agcagcatcc gcaggtaaaa gtacgcatta
     8161 tggaagggca actggtgtcg atgattaatg aattgcgtca gggagaattg gatttcacca
     8221 tcaataccta ttatcaggga ccgtacgacc acgaatttac ttttgagaaa ttactggaaa
     8281 agcaattcgc gatcttttgc cgcccgggac accccgccat tggtgcccgt tcgatcaaac
     8341 agttactgga ttacagctgg acaatgccga cgccacacgg cagctactac aaacagttga
     8401 gtgaattgct tgacgatcag gcgcaaacgc cacaggtcgg tgtagtctgc gagacgttct
     8461 cagcctgtat cagtctggtg gcaaaaagcg attttctcag catactgcct gaagaaatgg
     8521 gctgcgatcc cttgcacgga caggggctgg tgatgttgcc ggttagcgaa attttaccga
     8581 aagcggccta ttatttgatt cagcggcgtg atagtcgcca gacaccactg accgcgtcat
     8641 taatcacgca attccggcga gaatgcggct atctgcaaag ttaaaactgc ataaaaaaat
     8701 agagtctgtc gacatccgcc agactctaca gtacacacag cagtgcatcc gcgtcttaaa
     8761 tccggcattg tctcctctgc gccggtgact gtgttataac ggttttaacc tttagttgcc
     8821 aattttcttc agtgacagat ttcacgaaaa tataagttac attattaata tcgtgaatga
     8881 ataatcatgc ataagcattt tgcttaaaat atcggcaata tttggaactt attactggaa
     8941 ttttgggtaa tacgttgttg gaccgacccg gtctggttat catatcgcgc tcttaattgc
     9001 gggaggatgt aacatgaacc ttgacgacaa atcgctgttt cttgacgcca tggaagatgt
     9061 ccagccgctg aaacgtgcta ccgatgtcca ctggcatcca acgcgtaacc aacgtgcgcc
     9121 gcagcgtatc gacacgctgc agcttgataa tttcctcacc accggatttc tcgacatcat
     9181 cccactaagt cagccgctgg agtttcggcg ggaagggttg caacatgggg tgctggataa
     9241 gctgcgcagt ggtaaatatc cgcaacaggc gagcctgaat cttttgcgcc agccggtgga
     9301 agagtgccgc aaaatgatgt tcagtttcat tcaacaagcc atggctgatg gtttgcgtaa
     9361 cgtgctgatt attcatggca aaggacggga tgataaatcg catgccaata ttgtccgcag
     9421 ctatgtggcg cgctggctga ccgaatttga tgatgttcag gcatattgca ctgcgctacc
     9481 gcatcatggc ggcagcgggg cgtgttacgt cgcactacgt aaaacggcgc aggcgaagca
     9541 agaaaactgg gagcgccacg ctaagcgcag tcgttgatct cgagacgcat ccgcggctta
     9601 tgccgccagc acgcggttgc gtccatcatt tttcgcccga tacaaagcat catcaacgcg
     9661 tttaaacagt tcatcgatgc tttcatttcc ttcgtgatgc gccacaccaa tgctgacggt
     9721 aaagcgtggt aagcccgaaa tactcacttt tgccacgctt acacggatag tttcagccag
     9781 cgaaagcgcg gtatccagtg gggttcttgg tagcaataag acaaactctt cgcctcccca
     9841 acgaaacacc aaatcgcctt tgcgagcgca actttcaagg gtgcgggcga gggcgcataa
     9901 cacctcatca cctttagaat gcccatagag atcgttaatg tgtttaaaac gatcggtgtc
     9961 gatgagcaac aagctgtaat cctgggcgat ggcgaggtgc tgcatttggc ctggttccgt
    10021 aatgtgataa aactgtcgcc gattcagtaa tccggtcatc gcgtcatggt gagcagcatg
    10081 ttccagctgc tcctccagcc gtttttgctc agtaatatca tgcacaatac ataacatgag
    10141 cttgtcgcca taaatttcaa tcggcccggc ataggtctgc acatgacgag tagatccatc
    10201 cgccagttta tgaacaaaat tcaaaggttt atgaccgccg ggtaaatgcg aaatttcatg
    10261 catgataggc atgacgcgac gcccgagcat attaatttcc caggtatgtt tctggcacat
    10321 cgtttcatgg ttataaccat agaaattgag cgcggcgagg ttagcatcga cgatttggcc
    10381 atctcgtgag gggtcaatca acaacattgg tgcagagtta gtcagaaaaa agcgcgcata
    10441 aaaaccttgt tttttgcgct gataatttgc cgagcgactg gcttttaaac ccagcgttgc
    10501 cggcgcttcg ataccttcga aaataatcac cggttctgct tctgtcagct ttcgcaaaac
    10561 aagccgacag cttaatgctg tttcctcttc tttacgctga acagtgagga tttcgataat
    10621 atcgtgttgg ttttgcagat cggagaggta tttcggcagt tctttttgtg aggagacgga
    10681 atagggaccg gttcgtagct gactaaacgt gaggtcttgc atcaacagtc gcgccgcgct
    10741 attggcataa attaactgtt cctcaaaggg cgaaacgatc cagacaggac tggtgagtaa
    10801 gtccagggta ttgaagttgt gcgtaatcat tgagatcccg ttatttttat caatttttgt
    10861 tgctatccga tcgcaaaaaa gccatgtcat atgatcagat aattctgata atgataga
//
LOCUS       DAFAVF010000140        10812 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFAVF010000140 DAFAVF010000000
VERSION     DAFAVF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.140"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1137
                     /gene="narU"
                     /locus_tag="LGK42_003535"
     CDS             <1..1137
                     /gene="narU"
                     /locus_tag="LGK42_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBK2659743.1"
                     /translation="LLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPF
                     GIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLV
                     IFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIAD
                     QLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIA
                     RSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
                     LGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQKEAVTETAAALGFISAIGAVGGFFI
                     PQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1219..4959
                     /gene="narZ"
                     /locus_tag="LGK42_003536"
     CDS             1219..4959
                     /gene="narZ"
                     /locus_tag="LGK42_003536"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBK2659744.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGAI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIESIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4956..6500
                     /gene="narH"
                     /locus_tag="LGK42_003537"
     CDS             4956..6500
                     /gene="narH"
                     /locus_tag="LGK42_003537"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBK2659745.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6500..7195
                     /gene="narW"
                     /locus_tag="LGK42_003538"
     CDS             6500..7195
                     /gene="narW"
                     /locus_tag="LGK42_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBK2659746.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7192..7872
                     /gene="narI"
                     /locus_tag="LGK42_003539"
     CDS             7192..7872
                     /gene="narI"
                     /locus_tag="LGK42_003539"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBK2659747.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            7951..8844
                     /locus_tag="LGK42_003540"
     CDS             7951..8844
                     /locus_tag="LGK42_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBK2659748.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(8940..9785)
                     /gene="nhoA"
                     /locus_tag="LGK42_003541"
     CDS             complement(8940..9785)
                     /gene="nhoA"
                     /locus_tag="LGK42_003541"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBK2659749.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            9958..10527
                     /locus_tag="LGK42_003542"
     CDS             9958..10527
                     /locus_tag="LGK42_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK2659750.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10531..10758)
                     /gene="pptA"
                     /locus_tag="LGK42_003543"
     CDS             complement(10531..10758)
                     /gene="pptA"
                     /locus_tag="LGK42_003543"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBK2659751.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2523 a         2835 c         2913 g         2541 t
ORIGIN      
        1 ttattgcgtg ttccctactc ctttatggtg cctatattcg gtggacgccg atggacggtt
       61 tttagtactg caatcctgat tattccttgc gtctggctcg gaattgccgt gcaaaatccg
      121 aatactcctt ttgggatatt tatcgttatc gctttgctat gcggttttgc aggtgcaaac
      181 tttgcttcga gcatgggcaa tatcagtttc ttctttccaa aagccaaaca agggagcgct
      241 cttgggatta atggcggatt aggaaactta ggtgtaagtg taatgcagct ggttgcaccg
      301 ctggtcattt ttgtacctgt atttgccttt ctcggcgtca atggcgtacc gcaggccgac
      361 ggttcggtga tgtcgctggc gaatgccgca tggatttggg taccgctact ggcgattgcc
      421 acgatcgccg cctggtcagg gatgaatgat atcgccagtt cacgcgcctc aattgccgac
      481 cagctccctg tcttacaacg cctgcatctc tggctgctga gcctgcttta ccttgccacc
      541 ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac ccagttcccg
      601 gatgtgaata ttctgcgcct ggcgttcttt ggcccattta tcggtgccat cgcgcggtcg
      661 gttggtggtg ctatttccga taagttcggc ggcgtgcggg tgacgttgat caactttatt
      721 tttatggcga ttttcagtgc cctgctgttc cttaccttac cgggcacagg ctccggtaat
      781 ttcatcgcct tttacgccgt atttatgggg ctgtttctga ctgcgggtct gggaagtggt
      841 tctactttcc agatgatcgc cgtcatcttt cgccagataa ccatttatcg ggtaaagatg
      901 aaaggcggta gtgatgagca agctcagaaa gaagccgtca ccgaaacggc ggcggctctg
      961 ggctttatct cagccattgg cgcagtgggc ggctttttta ttccgcaggc gtttggcatg
     1021 tcgctcaata tgaccggctc tccggtcggc gcgatgaaag tgtttttaat cttctacatc
     1081 gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga agttcagcca aaaataagag
     1141 atcgatgcct gatgtacaac cgtgtcgcat ccgacaaggc atcagatccc aattcattcc
     1201 tggagcagga gtcatgtcat gagtaaactt ttggatcgct ttcgctactt caaacaaaag
     1261 ggcgaaacct ttgccgatgg tcacggacag gtgatgcata gcaaccgcga ctgggaggac
     1321 agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt ccacccacgg tgttaactgt
     1381 acaggctcct gtagctggaa aatctacgtt aaaaatggtc tggtgacctg ggaaatccaa
     1441 cagaccgact acccgcgcac tcgccctgac ctgcccaatc atgaacctcg cggctgcccg
     1501 cgtggcgcaa gttactcctg gtatctttac agcgctaacc gcctgaaata cccgctcatt
     1561 cgtaaacgac tgattgaact gtggcgcgaa gccctcaagc aacacagcga tccggtactg
     1621 gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga gctacaaaca agtgcgtggg
     1681 cgcggcgggt ttatccgctc caactggcag gaactaaacc agctgattgc cgccgctaac
     1741 gtctggacca tcaaaaccta cggcccggat cgcgttgccg gtttctcgcc gatcccggcg
     1801 atgtcgatgg tttcttacgc cgccggaacg cgttatctgt cactgcttgg cggcacctgt
     1861 ttaagcttct acgactggta ttgcgacctg ccgcccgcct cgccgatgac ctggggcgag
     1921 caaaccgacg taccggaatc tgccgactgg tataactcca gctacatcat cgcctggggg
     1981 tctaacgtac cgcagacacg tacgccggac gcccacttct ttaccgaagt acgctacaaa
     2041 ggcactaaaa ccatcgccat tacccctgac tactctgaag tggccaaatt gtgcgaccag
     2101 tggctggcac cgaaacaagg cactgatagc gccctggcga tggcaatggg ccatgtgatt
     2161 ttaaaagagt ttcatctcga taatcccagc gactacttta tcaactactg ccgccgctac
     2221 agcgacatgc cgatgctggt aatgctggag cctcgcgacg atggtagcta cgttcccggg
     2281 cgcatgatcc gcgcatctga cctggtggat ggactgggcg aaagcaacaa tccgcagtgg
     2341 aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc cgaacggtgc gattggtttc
     2401 cgctggggag aaaaaggcaa atggaatctg gaatccattg ccgccggtac ggaaaccgaa
     2461 ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag gcgtggcctt cccctacttt
     2521 ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca atccggtgct ggtgcgccaa
     2581 ttgcccgtta aaaacctgac gttagtcgat ggcaacacct gtccggtggt cagcgtttat
     2641 gatttggtac tggcgaatta cggcctcgat cgcgggctgg aagatgaaaa cagtgcgaaa
     2701 gattacgctg aaatcaaacc gtacacccca gcctggggtg agcaaattac cggcgtgccg
     2761 cgccagtata ttgaatccat cgctcgtgaa tttgccgata ctgcccataa aacgcatggg
     2821 cgctcaatga ttatcctcgg cgctggcgtc aaccactggt atcacatgga tatgaactac
     2881 cgtgggatga tcaatatgct gatcttctgc ggttgtgtcg ggcaaagcgg tggcggctgg
     2941 gcacactatg tcggccagga aaaactgcgc ccacaaaccg gctggttgcc actggccttt
     3001 gcgctcgact ggaaccgacc accgcgccaa atgaacagca cctcgttttt ctacaatcat
     3061 tccagccaat ggcgctatga aaaagtctct gctcaggagt tactttcacc gctcgccgat
     3121 gccagtaagt acagcggtca tctgattgat ttcaacgttc gcgccgaacg tatgggctgg
     3181 ctaccttctg cgccgcagct ggggcgtaac ccgctcggga ttaaagctga agccgacaag
     3241 gccggattat cccccacaga atttaccgcc caggcgctga aatcgggcga tttacgtatg
     3301 gcctgcgaac aaccagatag cggcagcaat catccgcgta atttgtttgt ctggcgctct
     3361 aacctgcttg gctcctccgg caaaggccac gagtatatgc agaagtatct gctggggacc
     3421 gaaagcggga ttcagggcga ggaactcggt gccagcgacg ggatcaaacc ggaagaagtc
     3481 gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc tggtgacgct cgacttccgc
     3541 atgtccagta cctgcctgtt ctccgatatc gttctgccca ccgccacctg gtacgaaaaa
     3601 gacgatatga acacctcgga tatgcatccg tttattcatc cgctttctgc ggcggtcgat
     3661 ccggcgtggg aatcacgcag cgactgggaa atctacaaag gtatcgccaa agcattttcg
     3721 caagtgtgcg tgggtcatct tggcaaagaa accgacgtgg tattacaacc cctactgcat
     3781 gactctccgg cagagctctc acagccgtgt gaagtgctcg actggcgcaa aggcgaatgc
     3841 gatctgattc caggcaaaac cgcgccgaat attgtggcgg tggagcgcga ctaccctgct
     3901 acgtatgaac gctttacctc gctcgggcca ttgatggaca aacttggcaa tggcggtaaa
     3961 gggatttcgt ggaataccca ggatgaaatc gatttcctcg gcaaactcaa ttacaccaag
     4021 cgtgatggcc cggcacaggg gcgtccgctg attgacaccg ccattgacgc ttcagaagtg
     4081 attctggcac tggcaccgga aaccaacggt catgttgcag tcaaagcgtg gcaggcgctg
     4141 ggcgagatca ccgggcgcga acatacccat ctggcgctgc acaaagagga cgagaagatt
     4201 cgctttcgcg atattcaggc gcagccgcgt aaaattatct ccagccccac ctggtccggt
     4261 ctggaaagcg atcacgtatc ctataacgcg ggatacacca acgttcatga gttaattccg
     4321 tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc atccgtggat gcgtgctttt
     4381 ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc gtagcgtcag tgagatgcgc
     4441 cagataccgc caaacggctt cccggaaaaa gcacttaact tcctgacgcc gcaccagaaa
     4501 tggggcattc actcaaccta cagtgaaaac ctgctaatgc tgacgctctc tcgcggtgga
     4561 ccgattgtct ggatcagcga aacagatgcc cgagaactga ccattgtcga taacgactgg
     4621 gtggaagtat tcaacgccaa cggcgcgctg actgcccgcg cggtggtcag ccaacgtgta
     4681 ccgccgggca tgaccatgat gtatcacgcc caggaacgca ttatgaatat tcctggttcg
     4741 gaagtaactg gcatgcgcgg cggcattcat aactcggtca cccgcgtttg cccgaaacca
     4801 acgcatatga ttggcggtta cgcgcagctg gcctggggct ttaactacta cggcaccgtc
     4861 ggatcgaacc gcgatgagtt catcatgatc cgcaagatga agaacgttaa ctggctggat
     4921 gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa aatccgttca caagtcggca
     4981 tggtgcttaa cctcgacaaa tgtatcggct gccatacctg ttcggtgacc tgtaaaaacg
     5041 tctggaccgg gcgcgaaggc atggagtacg catggtttaa caacgtcgaa accaaaccgg
     5101 gcattggtta tccgaaaaac tgggaagatc aggaagagtg gcaaggcggc tgggtccgcg
     5161 atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat gggcgtaata accaaaatct
     5221 tcgccaaccc ggtggtgccg cagattgatg attactacga acctttcacc ttcgactacg
     5281 aacatttgca tagcgcaccg gaaggcaaac atatccctac tgctcgcccg cgttcactga
     5341 ttgacggcaa acggatggac aaagtgatct ggggcccaaa ctgggaagaa ctgctgggcg
     5401 gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag gagatgtacg
     5461 ggcagtttga aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac tgcctcaatc
     5521 ccagttgcgt ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa gacggcattg
     5581 tgctgattga tcaggataaa tgccgtggct ggcgtttgtg cattagcggt tgtccgtaca
     5641 aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa atgtatcttc tgttatccgc
     5701 gaattgagtc aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc atccggtatc
     5761 tgggcgtgct gctttacgac gccgaccgca ttgaggaagc ggcgagcacc gagcgcgaag
     5821 ttgacctcta tgaacgtcag tgcgaagtat tcctcgatcc acacgatccc tcagtgatcg
     5881 aggaagccct gaaacaaggt attccacaaa acgtgattga agctgcccag cgttcgcctg
     5941 tctacaaaat ggcgatggac tggaaactgg cgctaccgct gcaccctgaa tatcgcaccc
     6001 tgccaatggt ctggtacgtt cctccgctgt cgccgattca gtcctacgca gatgcgggcg
     6061 gtttgccgaa aagcgaaggc gtgctgcccg ccatcgaaag cctgcgtatt ccggtgcaat
     6121 atctcgccaa tatgttgagt gccggcgata ccggtccggt actgcgggcg ctgaaacgga
     6181 tgatggcgat gcgccactat atgcgttcac aaaccgtgga aggcgttact gatactcgtg
     6241 ccatcgacga agtaggcctg agcgtcgccc aggtcgaaga gatgtatcgt tacctcgcca
     6301 ttgccaacta tgaagatcgt tttgtcatcc cgacgagcca tcgggaaatg gcgggcgatg
     6361 ccttcgcaga acgcaacggc tgcggtttta cctttggcga cggttgccac ggttcggaca
     6421 gtaaattcaa cctgttcaac agtagccgta tcgatgccat caacatcacc gaagtgcgcg
     6481 acaaagcgga gggcgaataa tgcagatcct caaagtgatc ggcctgttga tggagtatcc
     6541 ggacgagctg ttgtgggaat gcaaggagga cgcgctggcg ttgatccgca gcgacgcgcc
     6601 gatgcttacg gatttcaccc acaacctgct taacgcgccg ctgctggata aacaggccga
     6661 atggtgcgaa gtgtttgacc gtggacgtac cacatcgctg ctgctgttcg aacatgttca
     6721 tgccgagtcc cgcgatcgcg gccaggcaat ggtggacctg ctggcggagt atgaaaaggt
     6781 cggcctgcag ctggattgtc gggaactgcc cgattatcta ccgctgtatc tggagtattt
     6841 aagcgtactg cctgacgatc aggcgaaaga aggattgctc aatgttgcgc cgatcctcgc
     6901 cctgcttggc ggtcgcttaa aacaacgcga ggcaccgtgg tacgcgttgt ttgatgctct
     6961 gctgcaactg gcgggaagct ccctttcaag tgacagtgtc acaaaacaag taaacagcga
     7021 agagcgcgat gacacccgcc aggcgcttga tgcagtgtgg gaagaggaac aggtgaagtt
     7081 tattgaagat aacgccacgg cgtgtgacag ctcgccgctt aatcaatatc agcgacgctt
     7141 tagccaggat gtcgcgccgc aatatgtcga catcagtgcg ggaggtggga aatgattcag
     7201 tatctgaacg tcttttttta cgatatctac ccgtacattt gtgcgacggt gtttttcctc
     7261 ggcagctggc tgcgctacga ctacgggcag tacacctggc gcgcctcctc aagtcaaatg
     7321 ctcgataaac gcgggatggt gatatggtcg aatctgttcc atatcggcat tttggggatt
     7381 ttcttcgggc acttgttcgg catgttaacg ccgcactgga tgtacgcgtg gtttttgcca
     7441 gtggcagcga aacaactaat ggcgatggtg ctcggcggta tttgcggtgt gttgacattg
     7501 attggcggcg caggactgct gtggcgcagg ctgacaaatc agcgcgtgcg tgcaacttcc
     7561 actacgccgg atattatcat catgagcatt ttgctgatcc agtgtctgct ggggctaagt
     7621 accataccgt tttcagcaca gtatcctgat ggtagcgaaa tgatgaagct ggtgggttgg
     7681 gcgcaaagca ttgtcacttt ccgtggtgga tcgtcagaaa tgcttaacgg cgtagcgttc
     7741 gttttccgcc tgcatctggt attgggaatg accatcttcc tgctcttccc gttcaccaga
     7801 ttggtgcatg tgtggagcgc gccgtttgag tactttactc gtcggtatca gattgtgcgt
     7861 tcgcggcgat aatttcaccc tggccggata gcccttcgct ctccggccac ttgcaggcta
     7921 tgatagttct ccagtcacag gagaacgtta atgaaaccgc aggtgtacca cgtcgatgct
     7981 tttacctctc aaccatttcg cggcaattct gccggtgttg ttttccccgc cgataatctt
     8041 agcgaagcgc aaatgcagct tatcgcccgc gagttaggcc attcggaaac tgcttttctg
     8101 ctgcacagcg acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagtgccg
     8161 atttgcggtc acgcgacagt agctgcgcac tatgtacgtg cgaaggtgtt aggtttagga
     8221 aattgcacga tctggcaaac atcgctggca ggaaaacatc gcgtgactat cgaaaagcac
     8281 aacgatgatt atcgtatttc gctggaacaa ggtacgccgg gctttgaacc gccgctggag
     8341 ggtgaaacac gtgaggcgat tatcaacgcg cttcatctca ctgaggacga tattctgcca
     8401 ggcttgccaa ttcaggtggc aacaacagga cactcaaaag tgatgatccc gttgaaacca
     8461 gaagtggata tcgacgccct ttcgcctgat cttgctgcgc tgagcgctat cagcaaacag
     8521 attggctgca atggtttctt cccgttccag atccgcccag gcaaaaacga aaccgatggt
     8581 cgcatgttct cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc
     8641 ccgatgggtg catggttggt acatcacaac gtattgcccc acgatggcaa ggtgctgcgt
     8701 gttaaaggcc atcaggggcg cgcattgggg cgtgacggcg tgattgaagt gacggtgaca
     8761 attcgtgata accaaccgga aaaagtcacc atttcaggta cagcggtgat tttgtttcat
     8821 gccgaatggg caattgaaat ctgaaatcgt tgcctgatgc gcttcgcctg tcaggtctac
     8881 aaccgggcta atgtatgccg gataaggcgt ttacgccgca tccggcaacc tgacataaat
     8941 tattttcccg cctccggatg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca
     9001 gtaaagccat gtttcgcatc atccacgccc agaccaaact gttcctgcat cacggcatat
     9061 aacgacacca catccggtag atttcgctgc tccaccgcgt gcccattttc ataatgggta
     9121 aaatgaaaat tggtcagcgt tagcttgcca ccgtccggca aatggcggca catcagcaaa
     9181 tgatggcgaa aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg
     9241 ctttgttgct gctcgcagag atcaaaacgg tacatcgact gccaatgctg atgatgatta
     9301 aactgcaaca cccaatcatc accctcctgc aacaaccgat actctccgtg tggcgtggtc
     9361 tgcacgagat cggaaactaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca
     9421 tcagcaatcc atttttcctc ttccagttcc accaacagca aacgatgggt gcgcggcggt
     9481 aatgctggcg gatttgataa cactacgcgc cctaacaagc tgcgaacgtt aaactccagc
     9541 tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggct
     9601 atcaccagtt tctcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg
     9661 tcgaggtttt caaacggaat agtgcaattg tgtttcaggt gcaatgcacg aagcgtatca
     9721 atattgaccg cagcagctcc cgaccagtta atacgggcaa aatagtgatt cagaatgggc
     9781 gtcatcaggc gtttccctta gtggttttct caacttataa cctggaatag acgccgcacc
     9841 ggctattttg tgcgatcgcg gacatgcatc acgtttgcaa atcgccaatc ccgttaattt
     9901 cgttacttat tgcgttacgg actacgctta aaaaaacctt cttcgcgagg cgataccatg
     9961 agccgattca tccccataga attacaccat gccagccgtc tgttaaatca tggcccaact
    10021 gtcatgatca ccagtttcga tgagcaatcc cagcggcgta acattatggc agcagcctgg
    10081 tcaatgccgg tggagtttga accgccacgc gtggcgattg tggtagataa atcgacatgg
    10141 accagggagt tgattgaacg taacggtaaa tttggcatcg ttatcccggg cgttgcagca
    10201 actaactgga cgtgggcagt gggaagcgtg tcgggtcgtg aagaagataa atttaattgc
    10261 tatggcattc cggtggtgaa aggtccggtg cttggtttgc cacttgttga agagaaatgc
    10321 ctggcgtgga tggagtgccg cctgctacct gcgacttctg cgcaacaaaa atacgacacg
    10381 ctgtttggcg aggtggtatc agcagcggca gacgcacggg tatttgtcga aggccgctgg
    10441 cagtttgatg atgataagct caatacgttg catcatttag gtgctggcac gtttgttact
    10501 agcggcaagc gtgttacggc aggttaagca ttacatgctg taaccgggtt tctttatcaa
    10561 agcctccatt tggggcgcga tttcggcatc ccagatagct tgccaagatt ctggttgaat
    10621 ctgctgtaga gcaatgctta tcgaactgtc tttactgttc agatggcgaa taataacgtc
    10681 ggtaatatct gcagcaagtg ctgctttttg ttgttcgtcc agttcacgcg gaaagcattt
    10741 aatgtcgatg tgcggcatga ttgtgctcct tttgaaagat gtcagtaaaa ttaacacaca
    10801 ttgtgaattt tc
//
LOCUS       DAFAVF010000141        10809 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFAVF010000141 DAFAVF010000000
VERSION     DAFAVF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.141"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            155..721
                     /locus_tag="LGK42_003544"
     CDS             155..721
                     /locus_tag="LGK42_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBK2659752.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            725..1300
                     /locus_tag="LGK42_003545"
     CDS             725..1300
                     /locus_tag="LGK42_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBK2659753.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1342..2394)
                     /locus_tag="LGK42_003546"
     CDS             complement(1342..2394)
                     /locus_tag="LGK42_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK2659754.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2619..3539
                     /gene="lpxL"
                     /locus_tag="LGK42_003547"
     CDS             2619..3539
                     /gene="lpxL"
                     /locus_tag="LGK42_003547"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBK2659755.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3711..4937
                     /gene="mdtG"
                     /locus_tag="LGK42_003548"
     CDS             3711..4937
                     /gene="mdtG"
                     /locus_tag="LGK42_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBK2659756.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            5020..5394
                     /locus_tag="LGK42_003549"
     CDS             5020..5394
                     /locus_tag="LGK42_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBK2659757.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5395..5622)
                     /locus_tag="LGK42_003550"
     CDS             complement(5395..5622)
                     /locus_tag="LGK42_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBK2659758.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(5795..8338)
                     /gene="mdoH"
                     /locus_tag="LGK42_003551"
     CDS             complement(5795..8338)
                     /gene="mdoH"
                     /locus_tag="LGK42_003551"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBK2659759.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8331..9866)
                     /gene="mdoG"
                     /locus_tag="LGK42_003552"
     CDS             complement(8331..9866)
                     /gene="mdoG"
                     /locus_tag="LGK42_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBK2659760.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10260..>10809
                     /locus_tag="LGK42_003553"
     CDS             10260..>10809
                     /locus_tag="LGK42_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBK2659761.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIF"
BASE COUNT         2686 a         2802 c         2636 g         2685 t
ORIGIN      
        1 atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat tattgaaata
       61 aaaataaata tcattcaatt tggtattttt gtgttgcaat tcattctccg acacgccatt
      121 ttctttctct cgcaaattcg ttttggatgc acttatgtca ttcacaaata cccctgaacg
      181 ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt
      241 tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc
      301 cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg
      361 gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc
      421 cgcaaaagct ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta
      481 tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc
      541 gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc
      601 gtggagcgtc gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat
      661 cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata
      721 aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc
      781 agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatttccg
      841 catccagcac cttggctata gctggttata cggcaccttt aaagatttcg acggtacttt
      901 tacctttgac gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag
      961 cgtcgatact aatcacgccg aacgcgataa acatcttcgc agtgcagatt tcctcaatac
     1021 cgcaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact
     1081 ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa
     1141 attaattggt cagggcgacg acccatgggg tggtaaacgt gcaggcttcg aggccgaagg
     1201 caaaattaag ctcaaggact tcaatatcaa gacagattta ggtccagctt ctcaggaagt
     1261 ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg
     1321 gcacgcatcc ggcaatgata tttattccgt tggatcagga atgcccagtg ttgtattcag
     1381 acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg
     1441 ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta
     1501 tttttccgca catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt
     1561 ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat
     1621 acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc
     1681 ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt
     1741 gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta
     1801 catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc
     1861 acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata
     1921 gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc
     1981 cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc
     2041 aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa
     2101 cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa
     2161 tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata
     2221 cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt
     2281 aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc
     2341 caacattttg gcttttagcg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta
     2401 cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt
     2461 aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc
     2521 ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata
     2581 catacaattg cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct
     2641 ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt
     2701 tagtcgtgca attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg
     2761 cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc
     2821 cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca
     2881 tgggcctgat ggaaaccggc atggcgtggt tctggccgga ccgccgaatc gcccgctgga
     2941 cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt
     3001 tagttggcat ccattttctg acactggagc tgggtgcgcg gcagtttggt atgcaggaac
     3061 cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg
     3121 gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag
     3181 ccctgaaaaa aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa
     3241 gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc
     3301 tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca
     3361 aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa
     3421 ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt
     3481 atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa
     3541 tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt
     3601 tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt
     3661 atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct
     3721 gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt
     3781 ttcttaccgg tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc
     3841 ttggcgttac cggtcactcc gccctgaata tgtggtccgg tattgtcttc agcattacat
     3901 ttttattttc ggccatcgcc tcaccgtttt ggggtggact cgccgaccgt aaaggccgaa
     3961 aactcatgct attacgctct gccctcggca tgggcatcgt gatggtgttg atgggactgg
     4021 cacaaaatat ctggcagttt ttgatcctac gggcgcttct tgggttactt ggcggatttg
     4081 tccccaacgc taatgctctt atcgccacac aagtaccgcg taataaaagc ggctgggcgc
     4141 tgggtacgct ctccacaggc ggcgttagtg gtgcgttgct cggcccaatg gctggcggcc
     4201 tgctcgccga tagctacggc ttacgtccgg tattctttat taccgccagt gtgctcatac
     4261 tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt ccagccggtc agcaaaaaag
     4321 agatgctgca catgcgggaa gtggtgacat cacttaaaaa cccgaaactg gtactcagcc
     4381 tgtttgtcac cacgttaatc atccaggtag cgacaggctc aattgccccc attctgacgc
     4441 tgtatgtccg cgaactggcg ggtaacgtca gtaacgtcgc ctttatcagt ggcatgatcg
     4501 cctcggtgcc aggcgtggcg gctctgctaa gcgcaccacg actcggcaaa cttggcgatc
     4561 gaatcggacc cgaaaagatc ctgattacag cgctgatatt ttctgtactg ctgttgatcc
     4621 caatgtctta cgttcagacg ccattgcaac ttgggatttt acgttttttg ctcggtgccg
     4681 ccgatggtgc actactcccc gccgtacaga cactgttggt ttacaactcg agcaaccaga
     4741 tcgccgggcg tatcttcagc tataaccaat cgtttcgtga tattggcaac gttaccggac
     4801 cattgatggg agcagcgatt tcagcgaact acggtttcag agcggtattt ctcgtcaccg
     4861 ctggcgtagt gttattcaac gcagtctatt catggaacag tctacgtcgt cgtagaatac
     4921 cccaggtatc gaactgattt ttcgcctttc atacttgcaa aagcggagaa tcagctatcc
     4981 ttttccctga aacctcatca actcaaaggg agaatcgtga tgaccatgta cgcaacgctt
     5041 gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag acaaccccgg catcgacgcc
     5101 gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg ttttgcagga cggcgacatc
     5161 atgtggcaag ttgagttttt tgccgacgaa ggggaagaag gtgaatgttt acctatgctt
     5221 agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg atgagataga gatacgccag
     5281 gagtggcagg aagagaatac attacatgaa tgggacgagg gggaatttca gcttgagcca
     5341 ccgctggata ccgaggaagg acgcgcagca gctgatgagt gggatgaacg ttaatcactc
     5401 atacgggcca tgatgagtgt cgatcggcag cagtaaagta tcaaaaacca gagagaatgg
     5461 cagatcaagg atcgtgacat aacgccaggc ggagtcacgc acatcccatt gcacaccggg
     5521 ataatattgg ttgccatgcc cctgccccgg tatagtgcga ctaataatgc tgccacagcc
     5581 actcagcaag gtcaccatga tgctcaccac aattaaacgc atttgatacc tctttcgtaa
     5641 aaaaataccg gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgcggcgt
     5701 gaacgcctta tccgacccac aaaatcttgc aaaatcaata aattgcagga acgatgtagg
     5761 cctgataagc gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac
     5821 gcaatgccag tggattgagc tttatccctt cgtaataact cacccatgaa gaatatctct
     5881 ccggggaatt ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca
     5941 ccaggcgacg atcgcgattc agcttctctg gcgtctcgtt cagcgcctgt tcaacgtggc
     6001 ggtcacgggc gatttccagc accttactgg cgcggtgacg cgcggtcgcc attgcggttg
     6061 ccagagcgtt aaatgacggg ttaaacactg cgtgcataaa gccatcatca agggagcgtt
     6121 gacgattcat ctcaaggaac cgatcggtat caaccagcac ctgcggcggc gaatactctt
     6181 ccgggatcag gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa
     6241 tcaccgaaac aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga
     6301 aacgcagatc caaccacgcc ataccaacag cccacactaa ccctaacagc agctgtgagc
     6361 cgtggcgttt gaacgcttca ccccaggaag tggagtcatc atcacgctgc ggtgaattcc
     6421 acaccacttc ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta
     6481 ccggagccag cagcacggaa aaaagcactt ccagcagcaa cgataatgta acgcgccaga
     6541 agccgccgta ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca
     6601 ggaacaacag caccatagtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg
     6661 gccacaccgg gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgta
     6721 ctacctgcaa tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag
     6781 acatcacgcc cgtcaggaat accgcacggt gaaccgggtg catacccttc accaggaaca
     6841 gacggaagtt catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca
     6901 agttaggcgg caattcttca taagaacccg ggagatcgta agcaatccag accccccaac
     6961 ctgcacggcg catcaacgcc gcttccacga agtcatgtga caggattgaa ccggcaaagg
     7021 aaccttcgcc cggcagcgga gccagtgcgc agtgctcgat aaacggtttc acgcggataa
     7081 tcgcgttatg tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa
     7141 acagtggccc atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac
     7201 cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaggcgca
     7261 ccaggccgca taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc
     7321 tgtactggct gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc
     7381 ggcgacggcg gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca
     7441 tccaggcttt ttgctctgcg acgcagatat ccgggttata actgtcacta agaatgtaga
     7501 catcaaagtg tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaggccag
     7561 caaaaacacg gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg
     7621 ggtttaatgg ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa
     7681 gcagttgcag gaagcccatt aacgccgtcc agaatccggc ggacacccaa cagaacagta
     7741 ccgcaaagag gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa
     7801 cccacaaatc ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa
     7861 gaatggtctt catataccag gtcgcgacga cagtttgcgc gagcgtcagg atcaacagaa
     7921 tgtaacggcg gatggtaccg acggtacgcc acttttgctc gctctcctgc tcttctttgg
     7981 tcaaacgagc cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc
     8041 ctaccgggtt ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg
     8101 ccttcagctg atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg
     8161 gccaggcttg ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc
     8221 aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg
     8281 ccgctttctc gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg
     8341 gcaggtaact ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca
     8401 cgcatttcag tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct
     8461 ttggtaaccg ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg
     8521 gtttgcgctg tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg
     8581 accacaaagg cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc
     8641 gttgaacgac gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca
     8701 cggctgaagg tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc
     8761 ggcgtccagt aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc
     8821 tcaacgctgc ctttacccca ctcccctttc ggagtcaccc atgcgcttgg acgaagatcg
     8881 taacgatcat cgagatcttc aaagcgggag aaatcacgac cgcgctgcaa cagaccgaag
     8941 ccttgcgggt tctccatcga gaagctgctg accgctaaat gtttcgggtt attcaacgga
     9001 cgccagatcc attcgccatt accggcatgg atagacagac cgttagagtc gtgcaactcc
     9061 ggacgatagt tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac
     9121 ggtgcaaccc ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca
     9181 accgtgtcac gccctggcat aaccacgaat ttgtaagcac ctgtcgcgcg cggcgagtca
     9241 agcaatgcat aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct
     9301 ttgaagcgtg gaaattcttc acccgatggc aaggcggtat caattgccag gccgcgggca
     9361 gaaaggccat aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg
     9421 acgatttcat cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa
     9481 ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg
     9541 ctgtatttga ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg
     9601 aagtacatac cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac
     9661 gctttgtcat gattaaactg gatctgctga tagtccgcgt atttcatatc gcggaaaacg
     9721 gagggcaagt tgcttttggg cgcctcatag cctttcccgg ctaaggattg agcttgcttt
     9781 gcgacatcat caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca
     9841 gcactcaacc aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc
     9901 cccctttgtg tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa
     9961 cagaatcccg gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt
    10021 ttcactttcc gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca
    10081 attttattta tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct
    10141 atgcataaaa accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca
    10201 agcctaaagg cataacccga cagggataag acgaaaaatc cgagattagt taaccatata
    10261 tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt
    10321 tgttagggat cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata
    10381 gcgccgaacc atcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc
    10441 aggtattttt cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga
    10501 aaaaatggtg gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc
    10561 tactaacatt accgcaattt attatgctgc aatatgtcaa aggaaaagcg gaaagttggc
    10621 ctgggctgtc attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt
    10681 ggtttttact ggtgttagta gtcatgacga cgctgtgcgt atggatattt aagcgcatca
    10741 gaaataattt agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg
    10801 tgatttttt
//
LOCUS       DAFAVF010000142        10732 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFAVF010000142 DAFAVF010000000
VERSION     DAFAVF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.142"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            185..1567
                     /gene="dosC"
                     /locus_tag="LGK42_003554"
     CDS             185..1567
                     /gene="dosC"
                     /locus_tag="LGK42_003554"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBK2659762.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1592..3991
                     /gene="dosP"
                     /locus_tag="LGK42_003555"
                     /gene_synonym="pdeO"
     CDS             1592..3991
                     /gene="dosP"
                     /locus_tag="LGK42_003555"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBK2659763.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4249..4830
                     /gene="ddpX"
                     /locus_tag="LGK42_003556"
     CDS             4249..4830
                     /gene="ddpX"
                     /locus_tag="LGK42_003556"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBK2659764.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            4844..6394
                     /locus_tag="LGK42_003557"
     CDS             4844..6394
                     /locus_tag="LGK42_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK2659765.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6396..7418
                     /locus_tag="LGK42_003558"
     CDS             6396..7418
                     /locus_tag="LGK42_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2659766.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7415..8311
                     /locus_tag="LGK42_003559"
     CDS             7415..8311
                     /locus_tag="LGK42_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBK2659767.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8308..9294
                     /locus_tag="LGK42_003560"
     CDS             8308..9294
                     /locus_tag="LGK42_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2659768.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9287..10213
                     /locus_tag="LGK42_003561"
     CDS             9287..10213
                     /locus_tag="LGK42_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK2659769.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(10269..10700)
                     /gene="osmC"
                     /locus_tag="LGK42_003562"
     CDS             complement(10269..10700)
                     /gene="osmC"
                     /locus_tag="LGK42_003562"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBK2659770.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2604 a         2678 c         2775 g         2675 t
ORIGIN      
        1 accgaaaaat aattacgatg ggctatactc ttctgaagaa cagtagcgta gattaaaact
       61 gtcctaagtc gcttatttct tttcagtata tcttcatatt tcaggagaat atggtgcagt
      121 tagtcgatct cgcacgttgc gttttgattc cgtctgattt cccagcctta taagggtgtg
      181 aattatggag atgtatttta aaagaatgaa agatgagtgg accggacttg tcgaacaggc
      241 agatcctctc attcgtgcta aagccgcaga aattgccctt gcgcatgctc attatctgag
      301 tatcgagttt tatcgaattg tccgcatcga cccgcatgcc gaagaattct tgagtaatga
      361 acaagttgag cggcagttga aaagtgcgat ggaacgctgg attattaacg tgctttctgc
      421 ccaggttgac gatgtcgaaa ggctaataca aatccagcat accgtcgcgg aagtgcatgc
      481 ccgcattgga attccggtag aaattgtcga gatggggttt cgggtgctga aaaaaatcct
      541 ctatccggta atcttctctt cggattattc tgccgcagaa aaacttcagg tctaccattt
      601 ctcgattaac agtattgata tcgctatgga agtgatgacc cgcgcgttta cctttagtga
      661 cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc tcgttactgg aaaacgccga
      721 agaagaaaaa gaacggcaaa tagcctcaat actttcatgg gaaatagata ttatctataa
      781 aatcctgctg gattctgatt taggcagtag tttgccttta agccaggctg attttggcct
      841 gtggtttaac cataaaggtc gacattattt tagcggcatt gccgaagtag gccatatctc
      901 ccgtctgatt caggatttcg acggtatttt caatcaaacc atgcgtaaca ccaggaattt
      961 gaataacaga agtttgcggg tgaagtttct attacagata agaaataccg tatcgcaaat
     1021 tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc cacgaagtcg gtatggatgt
     1081 actgacgaaa ttacttaacc gccgtttcct gccgactatc ttcaaacgcg aaattgccca
     1141 tgccaaccgg accggtacac cgctgtcagt gctgattatt gacgttgata aattcaaaga
     1201 gatcaacgat acgtggggcc ataacactgg cgatgaaatt ctgcgtaaag tctctcaggc
     1261 cttttatgac aacgtccgca gtagtgatta tgttttccgc tacgggggcg atgaatttat
     1321 cattgttttg actgaagctt ctgaaaacga aacgttacgt accgcagaac gtattcgcag
     1381 tcgggtggag aaaaccaaac tgaaagccgc aaacggcgag gatattgccc tctcactttc
     1441 catcggtgcc gccatgttta atggtcatcc tgactatgag cgcctcattc aaatagccga
     1501 tgaagctctg tatatcgcca aaagacgagg tagaaaccgt gttgaactct ggaaagccag
     1561 tctttagatg cgccaggatg cagaggtaat catgaagcta accgatgcgg ataatgccgc
     1621 cgatggcatt tttttccccg cccttgagca aaatatgatg ggtgcggtgt taattaacga
     1681 aaatgatgaa gtgatgtttt tcaaccccgc cgcagagaag ctctgggggt acaaacgtga
     1741 agaagtcatt ggcaataaca ttgatatgct gattccgcgg gatttgcgtc ctgcgcatcc
     1801 tgaatacatt cgtcataacc gtgaaggcgg taaagcgcgt gtcgagggga tgagtcggga
     1861 gctgcagctg gagaaaaaag acggcagtaa aatctggacc cgttttgcgc tatcgaaagt
     1921 gagcgccgag gggaaagttt attacctggc gctggtacgg gatgccagcg tagaaatggc
     1981 gcaaaaagaa cagactcgac aattgattat tgccgttgac catctcgacc gaccggtgat
     2041 tgtcctcgat ccggaacgcc atattgtgca gtgcaatcgc gcatttaccg aaatgtttgg
     2101 ttactgcatt agcgaagcca gcggtatgca gcccgataca ctcctgaaca ttcctgaatt
     2161 ccctgccgat aaccgcattc gtttacaaca gttgctatgg aaaaccgccc gcgatcagga
     2221 cgaatttctg ctgttgacgc gcaccggtga aaaaatctgg attaaagcct ctatcagccc
     2281 ggtttatgac gtgctcgcgc atctgcagaa cctggtaatg actttctcgg atatcaccga
     2341 agaacggcaa atccgccagc ttgaaggcaa tattctcgcc gccatgtgca gcagcccgcc
     2401 atttcatgaa atgggggaaa tcatttgtcg taacatcgaa tctgttctca acgaatcgca
     2461 tgtttcgctg ttcgcactgc gcaacgggat gccgatacac tgggcgtcat cttcgcacgg
     2521 tgcagaagtt caaaatgcgc aaagctggtc agcgaccatt cgtcagcgtg atggcgcgcc
     2581 tgcggggatc ctgcaaatta aaacctcgtc aggagcagaa accagcgcct ttatcgaacg
     2641 cgtggcagat atcagtcagc atatggccgc gctggcgctg gaacaggaaa aaagccgcca
     2701 gcatattgaa caactcatcc aatttgatcc gatgaccggt ctgccaaatc gcaataacct
     2761 gcacaattac ctcgatgacc tggtcgacaa agccgtctct ccggtggtgt atctcatcgg
     2821 tgttgaccat attcaggatg tgattgatag ccttggctat gcgtgggccg atcaggcatt
     2881 gctggaagtg gtcaatcgct ttcgtgaaaa actcaaaccg gatcagtatc tctgtcgtat
     2941 cgaaggtacg cagtttgtcc tcgtgagcct ggaaaacgac gtcagtaaca ttacccagat
     3001 cgccgatgag ctacggaatg tggtcagcaa gccgataatg attgacgata aaccattccc
     3061 gcttaccttg agtattggca tcagctacga cgtgggtaaa aaccgcgatt acctactctc
     3121 cactgctcac aatgcaatgg attatattcg caagaatggc ggtaacggct ggcagttctt
     3181 cagcccggcg atgaacgaaa tggtaaaaga gcgtttggtt ttaggcgcag cgctgaaaga
     3241 agcgattagc aataaccaac tgaaacttgt ttaccagccg caaatcttcg cagaaacggg
     3301 tgaactgtac ggcatcgaag cccttgcgcg ctggcacgat ccccagcatg gtcatgtgcc
     3361 cccttcacgg tttattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg
     3421 ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc
     3481 ggcgctttcc gttaacttgt cggcactgca ctttcgcagt aatcaactgc ctaatcaggt
     3541 gtctgatgca atgcacgcct ggggtattga cggccaccag ctgacggtag aaatcacgga
     3601 aagcatgatg atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat
     3661 gggcgtgggc ttatcggtag atgattttgg tacgggcttt tccggattat cccgcttagt
     3721 cagtcttccg gtaacggaaa tcaaaattga caaaagtttt gtcgatcgtt gtctgacgga
     3781 aaaacgcatc ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac
     3841 cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg
     3901 tcgcgttatt cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg
     3961 gatgagcagc gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt
     4021 ttcctttaag gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt
     4081 ccttccttaa cttcccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa
     4141 agttaaaaaa tataacttta agccagcatt ctggcgcttc tcccggcatg gcatgagatc
     4201 tgcataagcg gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc
     4261 accgaactgg ttgatttagc cgtaatcttc cctgatctgg agatcgaatt gaaatacgcc
     4321 tgcgctgata acatcacagg taaagctatt tatcagcaag cgcgttgtct gttacacaag
     4381 gatgcgatta ccgcgctggc gaaaagtatc agcatcgccc agctgtcagg cttacaactg
     4441 gtgatttacg atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca
     4501 gacccgcaat atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgatc
     4561 gacctgacgc ttcgtgatga acacgggaac atcctcgata tgggcgcagg gttcgatgaa
     4621 atgcacgagc gctcccatgc ctatcaccct tccgtcccgc ccgccgctca gcgcaatcgg
     4681 ctgttgctga atgcgataat gactggtggc ggttttgtcg gtatctccag cgaatggtgg
     4741 cacttcgaat taccccaggc agcgagttac cctctgctcg ctgatcaatt cacctgtttt
     4801 atatcccctg gcacacaaca cgtcagttaa tcaggaaact gtcatgaaga gatcgatacc
     4861 gtttcgtccc acattgctcg cgctcgtcct tgccacaaat ttcccggttg cgcacgccgc
     4921 cgtaccgaaa gatatgctgg tgattggtaa ggccgccgat ccacaaaccc tcgacccggc
     4981 ggtaacaata gataataacg actggacggt gacctacccg tcttatcagc ggctggttca
     5041 gtacaaaacg gacggtgata aaggctcaac cgacgttgaa ggcgatctgg caagtagctg
     5101 gaaagcgtct gacgatcaaa aagagtggac gttcaccctg aaggacaatg ctaaatttgc
     5161 cgatggcaca ccggtcactg ccgaagcagt aaaactctct tttgagcggc tactaaaaat
     5221 cggccagggg ccagcagaag catttcccaa agatttaaag attgatgctc ccgacgaaca
     5281 tacggtgaag tttaccctta gccagccatt cgcaccgttc ctctacacgc tggcgaatga
     5341 cggtgcatcc attatcaatc cggcggtctt aaaggaacat gcagcggatg atgctcgcgg
     5401 cttcctcgcg caaaataccg ccggttccgg accatttatg ctgaaaagct ggcaaaaagg
     5461 tcagcaatta gttctggtgc caaatccgca ttaccccggc aataaaccga actttaagcg
     5521 agtatcggta aaaattatcg gtgaaagtgc ctcccgtcgc ctgcagctct cccgtggcga
     5581 cattgacatt gccgatgcgc tgccggtgga tcaactcaac gccctgaagc aggaaaataa
     5641 agtcaatgtg gcagagtatc cgtcactgcg cgttacctat ctgtatctga ataacagcaa
     5701 agcgcctctt aatcaggcgg atctgcgtcg ggccatttcc tggtctaccg attatcaggg
     5761 catggttaac ggcattctga gtggtaacgg aaaacagatg cgcggcccga ttccggaagg
     5821 catgtggggc tacgatgcga cggcaatgca atacaaccat gacgaaacga aagccaaagc
     5881 cgaatgggat aaagtgacga gcaaacccac cagcctgacg tttctctact ccgataacga
     5941 tccgaactgg gagcctattg ctttggcgac acaatccagt ctcaacaagc tgggcatcaa
     6001 tgtgaagctg gaaaagctgg cgaacgccac catgcgcgac agagtgggta aaggtgatta
     6061 cgacattgcg attggcaact ggagtccgga ttttgccgac ccgtatatgt ttatgaatta
     6121 ctggtttgag tcagacaaaa aaggtctgcc gggtaaccgc tcgttctatg aaaacagtga
     6181 ggtcgataag ttactgcgca atgcgcttgc gaccaccgac cagacgcagc gtacccggga
     6241 ctaccagcag gcacagaaaa tcgtcattga tgacgctgct tatgtgtacc tgttccagaa
     6301 aaactaccaa ctggcgatga acaaagaggt gaaaggcttt gtgttcaatc ccatgctgga
     6361 acaggtcttc aatatcaata ccatgagtaa ataacgtgac cttctggagt attttacgcc
     6421 aacgctgctg ggggctggtg ctcgtggtgg cgggcgtctg cgtgattacg tttattatct
     6481 cgcatctgat ccctggcgat ccggcgcggt tactggcggg tgaccgcgcc agcgatgcta
     6541 tcgtggaaaa tattcgccag caactgggac tggaccagcc actgtatgta cagttttacc
     6601 gctacgtcag cgacctgttt catggtgacc tgggaacatc cattcgtacc gggcgtccgg
     6661 tgctggaaga gttgcgtata tttttcccgg cgacgctgga gctggctttt tgtgccctgc
     6721 tgctggcact cctgattggc atcccgctgg gcatactctc tgcagtctgg cgaaatcgct
     6781 ggctggatca tctggtgcga ataatggcca ttaccggaat ctccacacct gcgttctggc
     6841 ttgggctggg cgtcattgtg ctgttttatg gtcatctgca aattcttccc ggcggcggaa
     6901 ggcttgatga ctggctggat ccaccaacgc acgttaccgg cttttatctg ctcgatgcgc
     6961 tgcttgaagg caacggtgaa gtcttcttca atgcgttgca acatctcatc ttaccggcat
     7021 taacgctggc gttcgttcac ctgggaattg tcgctcgcca gatccgctca gcgatgctgg
     7081 aacaattgag tgaagactac attcgtaccg cccgggccag cggcttgccc ggctggtata
     7141 tcgttttatg ttatgcgcta cccaatgcgt tgatcccatc gattaccgta ttgggtttgg
     7201 cgctgggcga tttgttgtat ggcgcagtgc tcaccgaaac cgtttttgcc tggcccggaa
     7261 tgggtgcatg ggtagtaaca tcaatacagg cgctcgactt cccggcagtg atgggctttg
     7321 ccgtcgtggt ttcatttgct tatgtgctgg tcaacctggt ggtggatttg ctctatttgt
     7381 ggattgatcc gcgtatcgga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc
     7441 cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg
     7501 cagtccgctc accgtgaccg gcgcagtcat cattgtatta atgctattga tgatgatttt
     7561 ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc
     7621 gccttctgcg gcgcactggt ttggcaccga tgaagtggga cgcgatctgt tcagccgcgt
     7681 actggtcggc agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat
     7741 gattggttcg ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat
     7801 catgcgcatc atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc
     7861 tgccgctctc gggccgagtt tgtttaacgc catgctggcg attgctattg tgcgaattcc
     7921 cttttatgtg cgcctggcgc gggggcaagc attagttgta cgccagtata cctatgttca
     7981 ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa
     8041 ctccctaccg ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc
     8101 cgccacgttg ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat
     8161 ggtagcaaat ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc
     8221 agcgattttg cttaccgccg tcgggtttaa tctctttggt gatggtattc gcgatctgct
     8281 tgacccgaaa gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg
     8341 catttgagtt tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag
     8401 attaaccgcg gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc
     8461 gcaatgctga ttatgcgtct gctacagacg ggcagttatt gcgtacatcg ggggcagatt
     8521 tcactgctgg gagacgatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc
     8581 gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc tcaatccgac acttcgaata
     8641 ggtcttcaga tgatggacgt gatccgccat catcaaccaa taagtcgtcg ggaagccaga
     8701 gctaaagcga ttgccctgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg
     8761 cgctatccgt ttgagctttc aggtggtatg cgccagcggg taatgattgc gctggcattc
     8821 tcctgcgagc cgcaattgat tattgccgac gaaccgacta cggcgctgga cgtcacggtg
     8881 cagttgcagg tactgcgttt gcttaaacat aaagcccgcg ccagtggaac tgcggttctg
     8941 ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat
     9001 gccggaagcg taattgaaag cggcgtgacg gcagatgtta tccatcatcc ccggcatccg
     9061 tataccattg gtttgctcca atgcgcacca gaacatggag taccacgcca gctattaccc
     9121 gccattccag ggacggtacc aaacctcacc catttgcctg aaggctgcgc ttttcgcgat
     9181 cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg cgctgacagc gtgtggtgac
     9241 aacaaccagc gctgcgcctg ttggtatccg cagcaggagg tcataagtgt ctgacacgtt
     9301 attaacgtta cgcgacgtcc atatcaattt cccggcccgt aaaaactggc ttggtaaaac
     9361 tacggaacat gttcatgcca ttaatggtat tgatttacag atccgccgtg gtgaaacctt
     9421 agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc gcacagcttt taatgggcat
     9481 gctgcaaccg agccacgggc agtacatccg ttcaggctca caacgcatta tgcagatggt
     9541 gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg gtgtggcgca tcatcacaga
     9601 accgctctgg atagctaagc gtagtagtga acaacagcgg cgagcgttag cagaggagtt
     9661 ggctgtgcag gtgggtattc gtccggagta tctcgaccgc ctgcctcatg cgttctccgg
     9721 cgggcagcgg caacgcatcg ccattgccag agcactctct tcgcagcctg acgtgattgt
     9781 gcttgatgag ccaacctctg cactggatat ctccgtgcag gcgcagatcc tcaatttact
     9841 ggtaacgcta caggtaaatc gcgggctgac ctatgtgctg atctcacaca atgtctcggt
     9901 gattcgtcat atgagcgatc gggtagcggt gatgtatctc gggcagattg tagaactggg
     9961 cgacgcgcag caggtgttga cggcacctgc acatccatac acccgattat tgctggattc
    10021 cctccccgcc attgataaac cgctggagga agaatgggca ttacgtaaaa cggatctgcc
    10081 gggaaaccgc acgttgccgc aaggctgttt tttctacgaa cgttgccctc tggcaaccca
    10141 cggatgtgaa gtccggcaat cattaacaat aagggaggac ggacgtgaga tccggtgctg
    10201 gcgggcgctg tagagatgct aacaacgcat caggcattca cgcgcctgac gcgtcatccg
    10261 gcaatgcttt acgatttcaa ctggtaatca agcgtaattt ccgctttcag tacctgagaa
    10321 accgggcatc ctgcttttgc tttctggatg atgccgtcaa aggtagaggc atcaataccc
    10381 ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg caaaaccggc atccacttta
    10441 tccagcgaca catcggcggt ggtatcaatc gatgttggcg taaatcccgc ttcccccagc
    10501 attaatgaaa gcgccattga gaaacatgcg gcatgcgctg cgccaatcag ttcttcaggg
    10561 ttagttcctt tttcgccttc aaaacgcgtg ttaaatccat acggctgttg gttcagcaca
    10621 ccactctcgg tggatactgt tcccttcccg cgtttgatat cgccttccca gtgtgcctga
    10681 cctttcttat ggattgtcat tgttgctctc ctgtgggctt aaaaaggaaa tt
//
LOCUS       DAFAVF010000143        10685 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFAVF010000143 DAFAVF010000000
VERSION     DAFAVF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.143"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            153..368
                     /gene="bdm"
                     /locus_tag="LGK42_003563"
     CDS             153..368
                     /gene="bdm"
                     /locus_tag="LGK42_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBK2659771.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            470..607
                     /gene="sra"
                     /locus_tag="LGK42_003564"
     CDS             470..607
                     /gene="sra"
                     /locus_tag="LGK42_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBK2659772.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            764..2461
                     /gene="maeA"
                     /locus_tag="LGK42_003565"
     CDS             764..2461
                     /gene="maeA"
                     /locus_tag="LGK42_003565"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBK2659773.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2595..3605
                     /gene="adhP"
                     /locus_tag="LGK42_003566"
     CDS             2595..3605
                     /gene="adhP"
                     /locus_tag="LGK42_003566"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBK2659774.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3752..4036
                     /locus_tag="LGK42_003567"
     CDS             3752..4036
                     /locus_tag="LGK42_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBK2659775.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4087..4740)
                     /gene="fdnI"
                     /locus_tag="LGK42_003568"
     CDS             complement(4087..4740)
                     /gene="fdnI"
                     /locus_tag="LGK42_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBK2659776.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4733..5617)
                     /gene="fdnH"
                     /locus_tag="LGK42_003569"
     CDS             complement(4733..5617)
                     /gene="fdnH"
                     /locus_tag="LGK42_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBK2659777.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5630..8677)
                     /gene="fdnG"
                     /locus_tag="LGK42_003570"
     CDS             complement(5630..8677)
                     /gene="fdnG"
                     /locus_tag="LGK42_003570"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8090..8092),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBK2659778.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8909..9790
                     /gene="yddG"
                     /locus_tag="LGK42_003571"
     CDS             8909..9790
                     /gene="yddG"
                     /locus_tag="LGK42_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBK2659779.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10049..10339
                     /locus_tag="LGK42_003572"
     CDS             10049..10339
                     /locus_tag="LGK42_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2659780.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            10363..>10685
                     /locus_tag="LGK42_003573"
     CDS             10363..>10685
                     /locus_tag="LGK42_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBK2659781.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGL"
BASE COUNT         2677 a         2735 c         2625 g         2648 t
ORIGIN      
        1 aatacctatc aatatcatga ggttaaaata taaaaatcat caaccaggac taatcttaac
       61 aacgaagcgg caaatatttg ccatgctgaa tgtgctttat aaatctgcga tccgtagcag
      121 acaccataaa tacacagaca cggagaatca ctatgtttac ttattatcag gcagaaaatt
      181 caacagcaga acccgctctg gttaatgcca ttgagcaagg ccttcgggca gagcacggtg
      241 ttgtcactga agatgacatt ctcatggagc tgaccaaatg ggtggaagct tctgataacg
      301 acatcctcag tgatatctac cagcaaacga tcaattatgt ggtcagtggc cagcacccta
      361 cgctttaagg tgctatgctt gatcggcaac ctaatttagg ggtttagcac gtgtttcttc
      421 gctacggcga tgttgtcctt aaaactagca acaggattga ggagttaaaa tgaaatcgaa
      481 ccgtcaggca cgtcatattc ttggactgga ccataaaatt tctaaccagc gcaaaatagt
      541 taccgaaggt gacaaatcca gcgtggtaaa taacccaacc ggcagaaaac gccccgctga
      601 aaagtaattc ataaccatca gtcctcaatg acgattaaac accattgcct gcgcaatggt
      661 gtttttgttt ttatctgctt tatacttggg gccgacgccc tggtggtaaa gcaaagacga
      721 taaaagcgtg ccagggatgg atattcaaaa aagagtgagt gacatggaac caaaaacaaa
      781 aaaacagcgt tcgctttata tcccttacgc tggccctgta ctgctggaat ttccgttgtt
      841 gaataaaggc agtgccttca gcatggaaga acgccgtaac ttcaacctgc tggggttact
      901 gccggaagtg gtcgaaacca tcgaagaaca agcggaacga gcatggatcc agtatcaggg
      961 attcaaaacc gaaatcgaca aacacatcta cctgcgtaac atccaggaca ctaacgaaac
     1021 cctcttctac cgtctggtaa acaatcatct tgatgagatg atgcctgtta tttatacccc
     1081 aaccgtcggc gcagcctgtg agcgtttttc tgagatctac cgccgttcac gcggcgtgtt
     1141 tatctcttac cagaaccggc acaatatgga cgatattctg caaaacgtgc cgaaccataa
     1201 tattaaagtg attgtggtga ctgacggtga acgtattctg gggcttggtg accagggcat
     1261 cggtgggatg ggcattccaa tcggtaaact gtcgctctat accgcctgtg gcggcatcag
     1321 cccggcgtat acccttccgg tggtgctgga tgtcgggacc aacaaccagc agctgcttaa
     1381 cgatccgctg tatatgggct ggcgtaatcc acgtatcact gacgatgaat actatgaatt
     1441 cgttgatgaa tttatccagg ctgtgaaaca acgctggccg gacgtgctgt tgcagtttga
     1501 agactttgct caaaaaaatg cgatgccgtt acttaaccgc tatcgcaatg aaatttgttc
     1561 ttttaacgat gacattcagg gcactgcggc ggtaacagtc ggcacactga tcgcagccag
     1621 ccgcgcggca ggtggtcagt taagcgagaa aaaaatcgtc ttccttggcg caggttcagc
     1681 gggatgcggc attgccgaaa tgatcatctc ccagacccag cgcgaaggat taagcgagga
     1741 agcggcgcgg cagaaagtct ttatggtcga tcgctttggc ttgctgaccg acaagatgcc
     1801 gaacctgctg cctttccaga ccaaactggt gcagaagcgc gaaaacctca gtgactggga
     1861 taccgacagc gatgtgttgt cactgctgga tgtggtgcgc aatgtaaaac cagatattct
     1921 gattggcgtc tcaggacaga ccgggctgtt tacggaagag atcattcgtg agatgcataa
     1981 acactgtccg cgtccgatcg tgatgccgct gtctaacccg acgtcacgcg tggaagccac
     2041 accgcaggac attatcgcct ggaccgaagg taacgcgctg gtcgccactg gcagcccgtt
     2101 taatccagtg gtatggaaag ataaaatcta ccctatcgcc cagtgtaaca acgcctttat
     2161 tttcccgggc atcggcctgg gggttattgc ttccggcgcg tcacgtatca ccgatgagat
     2221 gctgatgtcg gcaagtgaaa cgcttgctca gtattcgccg ctggtcctga acggcgaagg
     2281 tctggtacta ccggaactga aagatattca gaaagtctcc cgcgcaattg cgtttgcggt
     2341 tggcaaaatg gcgcagcagc aaggcgtggc ggtgaaaacg tctgccgaag ctctgcaaca
     2401 ggccattgat gataatttct ggcaagccga ataccgcgac taccgccgta cctccatcta
     2461 agcctgcgcc cggtagtgaa ggctaccggg ctatttctct ctcccttttt cagatctcat
     2521 ccatactggg tagtggcgaa taaatctcat ttgcctcacc tgctatgcag aacatcatcc
     2581 gaaaaggagg aactatgaag gctgcagttg ttacgaagga tcatcacgtt gacgttacgg
     2641 ataaaacact gcgctcactg aaacatggcg aagccctgct gaaaatggag tgttgtggtg
     2701 tatgtcatac cgatcttcat gttaagaatg gcgattttgg tgacaaaact ggcgtaattc
     2761 tgggccatga aggcatcggt gtggtggcag aagttggccc tggtgtcacc tcattaaaac
     2821 caggcgatcg tgccagcgtg gcgtggttct acgaaggatg cggtcattgc gaatactgta
     2881 atagtggtaa cgaaacgctc tgccgttcag ttaaaaatgc cggatacagc gttgatggcg
     2941 ggatggcgga agagtgcatc gtggtcgccg attacgcggt aaaagtgcca gatggtctgg
     3001 actcggcggc ggccagcagc attacctgtg cgggggtcac cacctacaaa gccgttaaac
     3061 tgtcaaaaat tcgtccaggg cagtggattg ctatctacgg tcttggcggt ctgggtaacc
     3121 tcgctctgca atacgcgaag aatgtcttta acgcgaaagt gatcgccatt gatgtcaatg
     3181 atgagcagtt aaaactggca accgaaatgg gtgcagattt agcgattaac tcacgcaccg
     3241 aagacgccgc caaaattgtg caggagaaaa ccggtggcgc tcacgctgcg gtggtaacag
     3301 cagttgctaa agctgcgttt aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg
     3361 tggctgtcgg tctgccgccg gagtctatga gcctggatat cccacgcctt gtgctggatg
     3421 gcattgaagt ggtcggttcg ctggtcggca cgcgccagga tctaaccgaa gccttccagt
     3481 ttgccgccga aggcaaagtg gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca
     3541 ccatctttac cgagatggaa gaaggcaaaa tccgtggccg tatggtgatt gatttccgcc
     3601 gttaagaggc ctttgctgcg agtgccagtc ctggtgtcgc agcatcgcac tctctccgac
     3661 attaaatggc taaatggatt attgttcctt cctacgcaac aataatgata gtgttcctga
     3721 tgaaggaaca ctactgagag taaagacact gatgaaaatg gccaatcatc cccgcccggg
     3781 ggacattatt caggaatcac tggacgaact taatgtcagc ctgcgcgagt ttgccagagc
     3841 aatggaaatt gctccctcaa cggcaagccg attgctgacc ggaaaagcag ctttgacacc
     3901 agaaatggcg attaaacttt ccgtggtgat cggcagttcg ccgcaaatgt ggctgaatct
     3961 gcaaaacgcc tggagtctgg cagaggcaga aaaaacggtg gatgtgtcgc gactacgccg
     4021 tctggttacg caataaaaaa atggcgcttc ttaaagcgcc attttcagct taaagttcaa
     4081 aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata
     4141 ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt
     4201 aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat
     4261 gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg
     4321 cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat
     4381 gatcgaccag aacatcatct tttgcccggc gttgtactta ccgacatccg ccactttatg
     4441 ctcattgcct ttcaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg
     4501 gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc
     4561 gaaaaacggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg
     4621 cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac
     4681 ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat
     4741 gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa
     4801 tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc
     4861 acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac agctccggct
     4921 gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc gggttgtaga
     4981 cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc gccagctcca
     5041 gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg
     5101 gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct
     5161 ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc
     5221 agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg
     5281 ggcacgcctt caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc
     5341 actccagctt gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc
     5401 tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt
     5461 tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa
     5521 gttttgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg
     5581 cggacctttt gataatgtcc tgcgtttcca tagccatctg ttcgccccct tacgccttct
     5641 cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg
     5701 gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg
     5761 gaatacccac cgtttcaacc tgctgaccat ttacattcag tggtttcaga cgacgcgtta
     5821 ccacagccac cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat
     5881 taatgccttt cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa
     5941 ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg
     6001 tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac
     6061 gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa
     6121 ttggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc
     6181 ccatcccttc cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga
     6241 agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg
     6301 gatcccacgg tttaccgttg atatccgccg aagcacggtt gtacagcacg cgacggttga
     6361 gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg gagttatcgc
     6421 ggttagccat ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg
     6481 cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa
     6541 tcagcacgcc atttgcgtca tagagatctt ccagcgcata gccgttgttc tcttttgcca
     6601 cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt
     6661 ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag atacccgcca
     6721 gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca
     6781 accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc gaaggcagac
     6841 ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc
     6901 agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca
     6961 ggcagctcac cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga
     7021 aataaccggt gactttgcct tcatccatca tgttgaaata cttgatgacg tcgtaggtct
     7081 ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat
     7141 agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat
     7201 cagctagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt
     7261 ctgacggcag cgtcagataa cctggcaggc tggtagagag caggcctaag tcagtcaagc
     7321 cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac
     7381 cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccaca gtgtgctgcg
     7441 tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca
     7501 cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca acaacgtccg
     7561 gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg
     7621 tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac gatttatcgt
     7681 attgccgttt ttcagcgtcg tagccgctga acagaccgtc ttcgaaagca aaatcatcac
     7741 gcaccagcag gctggcgttg gtgtaatgct taacgtattc ggcgttgatt ttgtcgtttt
     7801 cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg
     7861 cgtaaatatc cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg
     7921 catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag
     7981 cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca
     8041 tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg
     8101 cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgtgtc agcatcccgg
     8161 tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta
     8221 cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc agctttgcaa
     8281 tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat
     8341 attccgggta gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg
     8401 ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt
     8461 ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac
     8521 agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag
     8581 ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg
     8641 cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt
     8701 attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga
     8761 acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa tagcggtaga
     8821 aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt
     8881 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
     8941 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
     9001 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
     9061 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
     9121 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
     9181 ggcgattgaa gtgggtatgg tgaactatct atggcccagc ctgacaattc tctttgccat
     9241 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
     9301 cggcgtctgc tgggtgttag gcggtgataa tgggttacat tatgatgaaa tcatcaataa
     9361 tattaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
     9421 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
     9481 gctaacggga gcaagtctgt gggtttacta ttttctcacg ccacaaccag aaatggtatt
     9541 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc
     9601 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
     9661 tacacctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccac tgtcgttctc
     9721 gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
     9781 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
     9841 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     9901 acatatcgat aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta
     9961 ttttaaaaac agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt
    10021 ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac
    10081 tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa
    10141 actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga
    10201 tggtgataaa acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat
    10261 gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc
    10321 gcgcggcaag aatctctgat tgagttaagt gcccatcaca atatgatcac tgaccttata
    10381 ttacacaatc atcccaggat gaaaacaatc actttaaacg acaaccatat tgcacattta
    10441 aacgccaaaa acactacaaa actggaatat ttaaacttaa gcaataacaa tttactgcca
    10501 accaatgaca ttgatcaact aatatcatca aaacatcttt ggcatgtatt agttaacggc
    10561 atcaacaatg atccacttgc acaaatgcag tactggactg cagtaagaaa tataattgat
    10621 gacactaatg aagtgaccat tgatttatca ggacttaatt taaccactca accaccaggg
    10681 ctgca
//
LOCUS       DAFAVF010000144        10621 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFAVF010000144 DAFAVF010000000
VERSION     DAFAVF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.144"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            40..213
                     /locus_tag="LGK42_003574"
     CDS             40..213
                     /locus_tag="LGK42_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659782.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(285..434)
                     /gene="hokB"
                     /locus_tag="LGK42_003575"
     CDS             complement(285..434)
                     /gene="hokB"
                     /locus_tag="LGK42_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBK2659783.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            833..2473
                     /gene="trg"
                     /locus_tag="LGK42_003576"
     CDS             833..2473
                     /gene="trg"
                     /locus_tag="LGK42_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBK2659784.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVIRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSTQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(2511..3434)
                     /locus_tag="LGK42_003577"
     CDS             complement(2511..3434)
                     /locus_tag="LGK42_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659785.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3651..4994
                     /locus_tag="LGK42_003578"
     CDS             3651..4994
                     /locus_tag="LGK42_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK2659786.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPEDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5219..6874
                     /gene="opgD"
                     /locus_tag="LGK42_003579"
                     /gene_synonym="mdoD"
     CDS             5219..6874
                     /gene="opgD"
                     /locus_tag="LGK42_003579"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBK2659787.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7014..7238
                     /locus_tag="LGK42_003580"
     CDS             7014..7238
                     /locus_tag="LGK42_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBK2659788.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7301..7837
                     /gene="rimL"
                     /locus_tag="LGK42_003581"
     CDS             7301..7837
                     /gene="rimL"
                     /locus_tag="LGK42_003581"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBK2659789.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7832..8812)
                     /locus_tag="LGK42_003582"
     CDS             complement(7832..8812)
                     /locus_tag="LGK42_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659790.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNSWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8936..9928
                     /gene="tehA"
                     /locus_tag="LGK42_003583"
     CDS             8936..9928
                     /gene="tehA"
                     /locus_tag="LGK42_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBK2659791.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9925..10518
                     /gene="tehB"
                     /locus_tag="LGK42_003584"
     CDS             9925..10518
                     /gene="tehB"
                     /locus_tag="LGK42_003584"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBK2659792.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2714 a         2494 c         2701 g         2712 t
ORIGIN      
        1 tcaaatcgaa cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt
       61 ttgtttatgg gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt
      121 tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct
      181 attagtgcct cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat
      241 gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag
      301 gccatgagcg cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt
      361 cgggtcagga gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga
      421 gggttgtgct tcatagcctt tctccttgcc ggatggcggg taagaggcta agatctgaat
      481 tgctaggttc attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct
      541 gattcccggt tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt
      601 ccttagggac gaaaataatc acttcacgaa attgcgtgct gttttccaga atttttcgtc
      661 attcgggtta gccagtttag ccattcgtta ctctcttcat tccaatagca ttaattttct
      721 ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt tggtcataag taattaccgt
      781 caagtgccga tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac
      841 aactccctca cagcgattag gttttttgca tcacatcaga ctggttccgt tatttgcctg
      901 cattcttggc ggtatcttag ttctattcgc attaagttct gctctggctg gctatttcct
      961 ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt
     1021 agcgaacagt tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc
     1081 gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt gcgcaagccg aatcggagat
     1141 taaacagtcg cagcaaggtt atcgtgctta tcagaatcgg ccggtgaaaa cacctgctga
     1201 tgaagccctc gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc
     1261 tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt attaatcatg aaagtgagca
     1321 gatccgaccg ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag
     1381 caccagagcc aaccaactgg cggaactggc ccatcagcgc acccgcctgg gtgggatgtt
     1441 catgattggc gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgatacg
     1501 tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgctagtgg
     1561 cgatctgacg atgaacgatg aaccggcggg tcgtaatgaa atcggtcgct taaatcgtca
     1621 tttacagcaa atgcagcatt cactggggat gacagtaggg actgttcgac agggtgcgga
     1681 agagatttat cgtggcacca gcgaaatttc agctggcaat gcggacctgt catctcgcac
     1741 cgaagaacaa gcggcggcta tcgaacaaac tgccgctagc atggagcaac tcactgcgac
     1801 ggtgaaacag aatgcggata acgcgcatca tgccagcaaa ctggcgcaag aggcttctat
     1861 taaagccagc gatggcgggc agacggtttc cggtgtagta aaaacgatgg gcgctatctc
     1921 tacaagttcg aagaaaattt ccgagatcac cgccgtcatc aacagtattg ctttccagac
     1981 gaatattctg gcactgaatg ctgccgttga agccgcgcga gcgggtgagc aaggccgtgg
     2041 atttgccgtt gtcgccagcg aagtacggac actcgcaagc cgcagcaccc aagcggcgaa
     2101 agagattgaa ggcttgatca gtgaatcagt caggttaatt gacctggggt cggatgaggt
     2161 ggcaacggca gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg tcacacatat
     2221 catgcaggaa atcgccgccg cctcggatga acaaagtaga ggcataacgc aggttagcca
     2281 ggcgatttct gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc
     2341 agcggcggcg gtgtcccttg aagaacaggc ggcacgatta actgaggcgg tggatgtatt
     2401 ccgtctgcac aaacattctg tgtcggcaga acctcgcgga gcgggtgaac cagttagttt
     2461 cgctacggtg tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc
     2521 attgattttc gaatagcgtt aatcatcaac tggcaaccag aagagaacgt cgcatctacg
     2581 cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag ggccaccagc
     2641 gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga caaaccacac gtaatcgtat
     2701 tcaaccgtaa gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt
     2761 ttacagccct ggctctgcac taatgcatct gaatgctggc gtggcgcagt gccttctggt
     2821 gatacaacga ccggccattc cagcacccgg cttagcgtta cgttctcctg aagtagcggg
     2881 tgattagggc ggacaaccag cttcaacgat tcaagaaaca gcagttcgta attaagcccg
     2941 gtcatcagtt caggatctga catccgacca atgccgatat cgatttcccc ggtttttaaa
     3001 cccgccagaa tcatagggtt actcattgtc gcaacttgca aggtcgtctc tttttgttgc
     3061 tgatgaaact gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca
     3121 accctgacga catcattatt aagaccttct ttacgatgaa gcgactgtcc ggcagtgttg
     3181 atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc
     3241 gccccctgac gaccacgctc aaacaagcga gcgccagtca gctgctccag ttcattcaat
     3301 gtcttagaga gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct
     3361 tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga
     3421 ctattttttt ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt
     3481 tgattatgat aacttgattg caaaatatta ttaacaatta aagcaattat gttacagcaa
     3541 aatggataat attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgcaagag
     3601 tgaatgcggt tacctacact ccagattact gaccactgga ggcagacact atggcgaaca
     3661 gcatcacggc ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc
     3721 aagaagttcc gcaatatggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc
     3781 tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga
     3841 atgttgaacg tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc
     3901 ggatgtttgc cattatgggg atgtacccgg tgagctatta cgatctctcg caggcagggg
     3961 tgccggtaca ttcgacagca tttcggccca ttgatgatgc ttctctggcg cgtaatccct
     4021 tccgcgtttt tacctcctta ctccgccttg agcttatcga gaacgaaatt ttgcgccaga
     4081 aagcggcgga gattctacgt cagcgcgata tcttcacccc acgttgtcga caactgttag
     4141 aggaatatga gcagcagggc ggttttaacg aaacacaggc acaggagttt gtgcaggaag
     4201 ccctggaaac gtttcgctgg caccagtcag caacggtaga tgaagaaacc tatcgcgcct
     4261 tgcacaacga acatcggttg attgctgatg tggtctgttt tcctggatgc catatcaacc
     4321 acctgacacc acgtacgctg gatattgacc gggtgcagtc gatgatgcct gaatgcggaa
     4381 ttgaacccaa aattctcatc gaggggccgc cgcgccgcga ggtaccgatt ttactacgcc
     4441 agaccagctt taaagcactg gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc
     4501 ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc attaacgccg aaagggcgac
     4561 aactgtatga tgatcttctg cgtaacgctg gaaccgggca ggataatctc actcaccaaa
     4621 tgcatttaca ggaaaccttc cgcacttttc ctgacagtga gtttttaatg cgtcagcaag
     4681 ggctggcatg gttccggtac cgtctgacgc cttcaggtga ggcgcatcgt caggcgattc
     4741 atcccgaaga cgatccacag cccttaattg aacgtggttg ggtcgcggcg caacccatta
     4801 cctatgaaga tttcttgccc gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg
     4861 aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc atttgagcag gcgttgggtt
     4921 gtccggtttt ggatgagttc cagctttacc aggaagcgga agaacgcagt aaacgtcgct
     4981 gtggtttgct ttaaattctg accatccgcc tttgcaaaaa tttgcctgat tttacaaacg
     5041 aatcaggctc atcccatcga cataaaaaaa atgccgattt atgcatattc tctcagttca
     5101 acaattggat tattaataaa tattgtctag agtgagcggt cataaataag cactttcttg
     5161 ccgctgaaaa cgaccagcgc gggaccattc acaacaccag aaggactcac tttcaggtat
     5221 ggatcgtaga cgatttatta aaggttcaat ggctatggcc gccgtgtgcg gtaccagcgg
     5281 cattgcttct cttttttctc aggcggcatt cgcggcagat tctgatattg ccgacgggca
     5341 aacccagcgt tttgacttct ccattctaca gtcaatggcg cacgacttag cgcaaacagc
     5401 gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg acaatgacgc cgcaggctta
     5461 taacagtatt caatacgacg ccgaaaaatc gctctggcat aacgttgaga accgtcaact
     5521 ggacgctcag ttcttccata tgggaatggg attccgtcgc cgcgttcgta tgttttctgt
     5581 agatccagca acacatctgg cgcgtgaaat tcactttcgc ccggagttgt tcaaatacaa
     5641 cgatgcgggt gttgatacaa aacaattaga agggcaaagc gatctcggct ttgccggttt
     5701 tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta gtatcatttc tcggcgcgag
     5761 ttatttccgc gccgttgatg atacatatca atacggtttg tcggcccgcg gcctggcgat
     5821 cgacacttac accgacagta aagaagagtt ccccgacttt accgccttct ggtttgatac
     5881 ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg ccagcattac
     5941 tggtgcctat aagttcacta tccattgtga gaaaagtcag gtgattatgg atgtggaaaa
     6001 tcacctgtat gcgcgcaaag acattaaaca gctgggcatt gcgccgatga ccagtatgtt
     6061 cagctgcggt actaatgaac gtcggatgtg cgacaccatt catccgcaaa ttcatgactc
     6121 tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc tgaataatcc
     6181 gcaaaaattg cagttcaatg cttacaccga caacaacccg aaagggtttg gtttattgca
     6241 actggatcgt gacttctccc attatcagga cattatgggc tggtataaca aacgcccaag
     6301 tctgtgggtg gaaccgcgta acaagtgggg taagggcacc atcggcctga tggaaatccc
     6361 aacaacgggc gaaacgctgg ataacattgt ctgcttctgg cagccagaaa aagctgtaaa
     6421 ggcgggtgat gagtttgcat tccagtatcg tctgtactgg agtgcgcaac cgcctgttca
     6481 ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc cggaaggttg
     6541 ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg atttcgttgg
     6601 tggtgatctg aaagctgccg caccaaaagg cattgagccg gtgattacgc tttccagtgg
     6661 ggaagcgaag caaatcgaaa ttctctatat tgaacccatt gatggttatc gtattcagtt
     6721 tgactggtat ccgacttcgg actccactga tccggtcgat atgcggatgt atctgcgttg
     6781 tcagggcgac gctatcagtg aaacatggct gtatcagtat ttcccgccag cgccggataa
     6841 acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt tttcttcggc accttcttcg
     6901 ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt tccctaagtt aaatgagtaa
     6961 tctgatggtg tgtatttcag atacaccttg tcagccacta acagggagtg cgtatgtttc
     7021 cagaatatcg agatttaata tcccgtctga aaaacgaaaa tcctcgcttt atgtccttgt
     7081 tcgataaaca caataaactt gatcatgaaa ttgccagaaa ggaaggttcc gacggtcgag
     7141 ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa gatgagatgc
     7201 tcaaaatcct gcagcaggag agcgtcaaag aggtataaac tttcctaagc cgccagccag
     7261 gcggcttttt taacaactgc atggattgac tggagataag atgactgaaa cgataaaagt
     7321 aagcgaatca cttgaattac atgctgttgc agaaaatcac gtcactcctc tttatcagtt
     7381 aatctgtaaa aataaaacct ggttacagca gtcgctaaac tggccgcagt tcgttcagag
     7441 tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc
     7501 caaaatgttc atgattttca aagaagatga acttatcggc gttatctcgt ttaatcgtat
     7561 tgaaccactg aataaaaccg ctgaaatagg ctactggctg gacgaatctc atcaggggca
     7621 ggggatcatt tctcaggcgc tgcaggcatt gattcatcat tacgcccagt ctggtgaact
     7681 tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa agcaaccagg tcgctttgcg
     7741 caatggtttt atccttgaag gttgcctgaa acaggctgag ttcctgaatg atgcctatga
     7801 tgatgtgaac ttatacgcgc gtattatcga ttcataataa ccctgccagc ggcgttcgcg
     7861 taatgcgctc ctcgccgtta atgacttttg gaccacggac atgaaccgtg tcgccatcaa
     7921 aggcttcaac gacggcgtgg tcggttaact ccacatggtt ttcaattatc accgcacctg
     7981 taatacggct ctcaccttgt attacaacgt gctcgtcgag cagaatcggt ccaccacgta
     8041 ccacggcatt tccgccaatc aagacatgat gttttaacac acaattacct tccacaatgg
     8101 catattccgc cacctgcgaa ctgtaatgaa tcgttggaat ggcatcttct tctatgccag
     8161 ctttcacctg cgcgtgaccg tagactttag cgcaatcgca tagccagacg ttgttctctt
     8221 cattaccttc aatactggca aaatcaaaaa cttcggcacg atgttcaata aaagcatacc
     8281 ggatgacggc atcgccataa atttgtgcct ggtggacaat acgcgaggcg ctaacccttg
     8341 cgcgatcata aatttgtagc aggagttgat ggtcaggcgt taagccttgt gctgcgacga
     8401 tcatagaatg ttgattaatt aaggcgtgac caaatattct acattgcccg caaaccagtg
     8461 agtcatgaat ggtgacactg tcacttatat atgcaccctg gctgatttca ctattatcga
     8521 tccagacatt atccgttgcg taaacctctc cccacagtac gctggtgccg gtgatgcgcg
     8581 tattaccgga aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa atccaggaat
     8641 ttccttcctg tgctaacacc gtttcgcgat cgatccagcc gccagcggtt cctgctatca
     8701 catcgttaaa atcgctaatg gcgataatct gccgtaataa cacatttttt ttagtgccat
     8761 cttcctgata actaaaggct cgctgttctt cacttagacg atatttgcgc atagcgtttt
     8821 cccacaggtg acttactata accgtagcaa attctgcggc tctggctatg ctcgagaaaa
     8881 ttccataaaa tgcatttcaa atatacttta taaattaaac aaaatgagta agaagatgca
     8941 gagcgataaa gtgctcaatt tgccggcagg ctactttggt attgtgttgg ggacgatagg
     9001 gatgggattt gcctggcgct atgccagcca ggtttggcag gtcagccact ggttagggga
     9061 tgggctggtg attctggcga tgatcatctg gggattatta actagcgcat ttattacccg
     9121 actcatacgc tttccgcata gcgtgctggc ggaagttcgc catccagtgc tgagcagttt
     9181 tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt ggttttgttc cgtggtttcg
     9241 cccactggcg gtgtgcctgt tcagttttgg tgtcgtggtt cagttggctt atgccgcctg
     9301 gcaaactgcg ggattatggc gcggatctca ccctgaagaa gctaccacgc ctggactgta
     9361 tctgccgaca gttgccaaca actttatcag cgcaatggcc tgtggtgcgt tgggctacac
     9421 cgacgccggt ctggtgtttt taggcgcagg cgttttctca tggctaagcc ttgaaccggt
     9481 gatcttgcag cgtctgcgca gttcgggaga attacccacg gcactgcgga catcactcgg
     9541 cattcagctc gctcctgcgc tggtggcttg tagtgcctgg ctgagcgtca acggcggcga
     9601 gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg ctgcaactgc tgtttatgct
     9661 acgtctgatg ccatggtatc tctcccagcc atttaatgct tcattctgga gtttctcgtt
     9721 cggcgtatct gcactggcaa ccaccggttt gcatctgggg agtggcagcg ataatggatt
     9781 tttccatacg ctggcggtgc cgctgtttat ctttaccaat tttattattg caatactgct
     9841 catccgtact tttgcgcttc tgatgcaggg aaaattgtta gtcagaaccg agcgcgccgt
     9901 tttaatgaaa gcagaggaca aagaatgatc attcgtgacg aaaactattt tactgataaa
     9961 tatgaattaa cccgcacaca ctctgaagta ctggaagcgg tgaaagtggt taaaccgggt
    10021 aaaacgctgg atctgggctg tggcaatggt cgtaacagtc tttacctggc agccaatggt
    10081 tatgatgttg acgcatggga taaaaatgcc atgagtatcg ccaacgtcga gcgcattaaa
    10141 tccattgaaa atctggataa tttacacacc cgagtcgttg atctgaataa cctcacattt
    10201 gatagacagt acgattttat tctttcgact gtggtactga tgttccttga ggctaaaacc
    10261 atcccagggc tgattgtcaa tatgcaacgt tgcactaaac ctggtggtta caacctgatt
    10321 gtggcggcga tggataccgc tgattatcca tgtaccgtcg gcttcccgtt tgccttcaaa
    10381 gagggagaat tacgtcgata ttacgaaggc tgggagaggg tgaaatacaa tgaagacgtc
    10441 ggcgagctgc accgcaccga cgccaacggt aatcgtatta aactgcgttt cgccacgatg
    10501 ctggcacgta aaaaatgacc cgctaagaat aaggcgcgtg aaaattcccc acgctgagat
    10561 gatttactgt tcttcttttc ggtaagcata ttttttatcg aagggatgtg aaattaatca
    10621 c
//
LOCUS       DAFAVF010000145        10568 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFAVF010000145 DAFAVF010000000
VERSION     DAFAVF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.145"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(82..312)
                     /locus_tag="LGK42_003585"
                     /pseudo
     CDS             complement(82..312)
                     /locus_tag="LGK42_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            369..1037
                     /locus_tag="LGK42_003586"
     CDS             369..1037
                     /locus_tag="LGK42_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2659793.1"
                     /translation="MVKLMKKNTDDGARIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1092..1676)
                     /locus_tag="LGK42_003587"
     CDS             complement(1092..1676)
                     /locus_tag="LGK42_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2659794.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1676..5206)
                     /locus_tag="LGK42_003588"
     CDS             complement(1676..5206)
                     /locus_tag="LGK42_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBK2659795.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGADIPDKGRFLNNINAV
                     SKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELASRVIITTATRTAGDP
                     MNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYVDGAA
                     FPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVILDFKSGRGFYESNS
                     LIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKTHLLLTNQGDVYGGW
                     NTLRPFSIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGVEFDGSKDITLTAAH
                     VAAFARRATDTYADADGGVPWNAESGAYNVTRSGDTYILVNFYTGVGSCRTLQMKAHY
                     RNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQA
                     FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDL
                     GTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTS
                     SGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIG
                     SHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(5271..5870)
                     /locus_tag="LGK42_003589"
     CDS             complement(5271..5870)
                     /locus_tag="LGK42_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2659796.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLQVTDNTGKTHDVLIGSNDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(5938..9417)
                     /locus_tag="LGK42_003590"
     CDS             complement(5938..9417)
                     /locus_tag="LGK42_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2659797.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTSVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVHHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHNYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFCGAKHERENNDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            complement(9478..10110)
                     /locus_tag="LGK42_003591"
     CDS             complement(9478..10110)
                     /locus_tag="LGK42_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2659798.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(10047..>10568)
                     /locus_tag="LGK42_003592"
     CDS             complement(10047..>10568)
                     /locus_tag="LGK42_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK2659799.1"
                     /translation="PWLSEADRRLQVQSDLPWWLVCRGTIHKFRCVPHLTGRRFEHGV
                     TDCYTLFRDAYHLAGIEMPAFHREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVL
                     LCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GIYNDLVAASTFV"
BASE COUNT         2323 a         3066 c         2565 g         2614 t
ORIGIN      
        1 atcaaatcaa tatacatttt gcaccttcga gatagtaatg gccattactg taacatgagt
       61 tacaaacagc ttcataccca gagctactga tgtatcaaca cgattcaaca atacccgata
      121 tttattccat gcctccagca acgatctttc ttcctccgtt gcgatttcca gatctacagc
      181 atcctgcagt ggcgcaatat acccactgaa ttcctggatg tagaactgtg tggtgacggt
      241 cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa tatcgctatg
      301 ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt cacaaataaa
      361 actataacat ggtgaaatta atgaaaaaaa acacagatga tggggctaga atttacacac
      421 cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg cttgcatggg
      481 gatgtcctac aaaggaacac cttcttccac actttctgga acatttaggt aacaaccatc
      541 tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt agtctgatat
      601 ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct ggggaatcaa
      661 aaattaaaca taaaattagc catgatgttt ttgaacctta tcccgcggcg ttacatggtc
      721 aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat atatctacaa
      781 aaagctgtgt aatacgcaat gcggcgcagg ccttaactga cgatggaact ctatacggag
      841 ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg atgcgcattt
      901 acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta acacatatac
      961 tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta atgttttccg
     1021 cttcagggaa aaaatagcat ccaaccgcag cacattcttg cttaagacgt gctgcggcat
     1081 aatcccaatg attactccct gacagggttc gtaggccact caatatcagg tgcagttgat
     1141 gtatcaacac ggttcagcaa cacccgatac tttttccagg cttccagcaa tgaggtttct
     1201 tcctccgttg cgatttccag atctacagca tcctgaagtg gcgcaatatg ctcactggct
     1261 acctgcatca ggctgttttt tgtttcttcc gcctcccgga tccggaacag tttttctgct
     1321 tccgtatcct tcacccaggc tgtgccgttc cacttctgaa actccccttc cggcgataac
     1381 caggtaacat tttccggtaa cggaccgagt tcagaaataa ataacgcgtc gccggaagcc
     1441 acgtcataaa ccgttttacc ccgatgatct tcaacgagat gccacgatga ctcatcactg
     1501 ttgaaaacag ccacaaagcc agcaggaata tctggtggtg caatatcggt actgtttgct
     1561 ggcagacctg tatgaggcgg aatatatgcg tcaccttcac caataaattc attagttccg
     1621 gccagcagat tataaatttt tatggtccgt ggttgttcac tcattctgaa tgccattatg
     1681 caagcctcac aatatagtta aatgcgatgt ttttgacggt gttttccgcg ttaccagcag
     1741 cgttaacggt gatggtgtgt ccatgtgaac caatcgcaac ggagtgcgta tgagcaccaa
     1801 taccgacagt atgcgcgtgt gcacctgcag atgctgctgt gccggacagt gaatggctat
     1861 gattaccatc tgtactggta ttcgctaacc accccgtaga catacctact gagccttgta
     1921 caccccaggt attttgacct gagcttgtat aaccatattg ataagtatct ttaaaaacac
     1981 tggggttaaa tcgacggcca tctctatggc tgtgattacc agctgcattc gtgctgccac
     2041 ttaaactatg ggtatgcgca ccagtgttat tcgtggattt agtgccgtaa tcaaacgacg
     2101 atgtggtttt cgtccccaaa tccgtactgg atgcgctggc gctgtgggta tgcgatttaa
     2161 tgccgtcctg ttcctgagac aatacggccc gaccactggc aggtttgccc ttaatcgtcc
     2221 agccacgcat atcagggatc acgcctgacg gataagccgc tgcaagtttc gggtatgcag
     2281 atttgtcaaa agcctgcccc tgcatcaggg cataaccaga cggaacggta tctgatggcc
     2341 acgggattgg tgcgccgact gggtagcttt ctggtggaag atttttcgag gtataaactt
     2401 ctgcccagtc ttcctcaaaa ccatagccat ctcttgagga acggtagaac agacctccat
     2461 ttctgtaatg tgccttcatc tgcaaggtcc ggcaacttcc gactccggta tagaagttaa
     2521 ccagaatata ggtgtcgcca gagcgggtga cattgtaagc gcctgattcg gcattccagg
     2581 gaacgccacc atccgcatcg gcatatgtat ccgttgccct tctggcaaaa gcagccacat
     2641 gcgcggcggt taaagtaata tctttggaac catcaaactc aacaccagaa acccgtcttg
     2701 gcgtttgcag ctttgttgct gttaatgcat taccgttcag acttgcggac agtttggttc
     2761 caataaccag ttcgccggtt gcgttatcaa tagaaaacgg tcttaatgta ttccagccac
     2821 cataaacatc accttgattg gtaagcagca ggtgagtttt agcgccatca ttacgccata
     2881 atgcaccata ctccccacct atcattcgaa tctgattacc accacgcgct acaatttcgt
     2941 ctgtggcaaa aagttttttg cacgacaagt tatcgttaac gattaacgaa ttagactcat
     3001 aaaaaccacg cccactctta aaatcaagga taacgtccgc cgcgatacat tcagtcgccg
     3061 gatttgttgc cccaaactta taggtcgtat cattaacaac gagatcagca ccaggtgcgg
     3121 atattgacag gccatcttcg ataaacgcaa aaacagggaa agctgcgcca tcaacataga
     3181 acacagagcg caaatcatcg cccttattac tcatcattat tgagtggatg gctcgttcat
     3241 tgttttgata ttgccagaac attccataag cataacgccc cctgtcagtc cagccgccag
     3301 gcataacaaa tccgttaaac tcgcagttat tcatcggatc gcctgcggtt cgcgttgccg
     3361 tggtgataat gacccttgat gccagttcgc ttactgagcc agcagaacgc ataacaacaa
     3421 cagggtaata ttttccagat gttgcacctg caggagcgtt aacccgcaca taacgcatac
     3481 cacgcttatc agcaaagtct gttttactga ccgcgttaat gttgttcagg aagcgtccct
     3541 tatcgggtat atcagcgccg ttctggtctt tctgcagacg tttctctgca ttgtcatagg
     3601 ctgattttac tgcctttggc gttgccgcca gcgtttcaga cgtactgttg gtcgcactgc
     3661 ttagctgaac tatccctttc tgtgctgtcg ttgcatcctg tgcggtgtat ttcccgttag
     3721 ccaggtcata cgcggcctta acggcttttg gtgttgccgc cagtgactcg gaagtgctgt
     3781 tagtcgcact actgagctgt actatcccct ttttcgtcgt gctcgcatcc tcaagcgcca
     3841 cggcggatgc aatatcctct gcccgttttg ccgctgtctc agcgcgcgtt gctgcagatt
     3901 ccgccgtact tttgctctga gctgctgccg tcgcactgcc agcagcctct gtcgccttcg
     3961 tggatgccgt cgtggcgctg cccttcgctg ctgacgcctg tctggtcgcc tcatcttttg
     4021 aagcagacgc cgatgatgcc gatgacgccg ccgaactggc ggacgatgcc gctgccgttt
     4081 ttgaggattc tgcacgggtt tccgacgctt ttgcgttcgt ttcggatgtc ttcgctgcgg
     4141 aagcagacct cgctgctgcc gtggcctgct cagtggcttc gccagccttc gttgtggctg
     4201 ttgaagcaga cgatgcggca ctttctgccg actttccggc agcggtggca ctggctgagg
     4261 cctgcccggc acttgttgac gcggcgctgg cagatgatgc agccgctgtt tttgagcctg
     4321 ccgcagccga ggcgctctgt cccgctgccg tttcagaaga cctggcgttc gtctcggacg
     4381 tttttgccgc cttcgcggaa tttcctgccg ccgttgccga ggaagcggca ctactggcgc
     4441 tcgatgatgc gttcgtttct gatgattttg ccgcctcttt tgaagccgac gcatcccgtg
     4501 ctgaagtggc tgcttctgac gctttcgtgg tcgcggtgga tgcagaagtg gctgctgatt
     4561 gttgtgacgc tgccgcattc gtttctgacg ttttcgccgc accggcactg gtagccgccg
     4621 cgctttttga ggactctgca gcggcagcac tttttaatgc ttcagtggcc tttgttgatg
     4681 ccgttcctgc gctggaagac gctgactgag ccgacgaagc agcctgtccg gctgacgtgc
     4741 tggctgcgcg tgctgagcct gcagcatcag tcgcatgggt tgccgcctca cgggctgatg
     4801 tgctggcatc gctggctgac ttcttcgcgg ccgccgtgtt ctgtgccacc gcggacgcgt
     4861 tacgcgccac ctcttccacc atcagttcaa aacggcgcag tgcctcagga cgggcatcat
     4921 cctccgtcat ggcaccgaga aaatcattca gcgtaccggg tcgggaatct tcatacacgg
     4981 tgatggtccc ggcatgtgac ggcgggaatc cttccaccaa cagaataacg ctgtactgac
     5041 cgtactcaac gtccatgctg taacgcccgg cttcatccgg attttctgag gccagcgtgt
     5101 tcaccaccac agtggtactg ttacgttttg ctttcagctg gattgtgcag ttctgtaccg
     5161 gttttcctgt gccgtctttc agtacacctg aaatctttac tgccatattc accccacaaa
     5221 aaagcccgcc tgaaccggcg ggctgtcata acactgtgtt acctggctaa tcagaattta
     5281 taaccgacac ccacgatgaa accgtcagtg cgccagtcgc cactgccgga accttcataa
     5341 gcgacatcaa cggccacgga ttcggtcggg ttaaactgca cgccagcccc ccacgccaga
     5401 gacgtgttgc tgtggcgacc gtcattactt ccgatcagca catcgtgcgt tttccccgta
     5461 ttgtcggtca cctgcagata atccccggag aaagtcgaca cacggctgta agccacaccc
     5521 gccatcgcat acgcgctgaa ccattcattc acgcgcacag acggccccgc catcacgctg
     5581 aaccagcggt tacgcacgga atcttcatgc cagcgggtat cgctgtaatg cgttttttgc
     5641 tcatcttcag cgttggcata actgaatgac gtaatcagcc ccagcgtgtc cgtaaactca
     5701 taacggtatt tcacgttaat cccgttcaga ttatcgctgc cgggagcgtt cgtacgggca
     5761 tgaagatacc ctgcgctcag tgtggcctgc tgctcagacg cccatgcagg cgcaccggat
     5821 acggccagac agatggctgc ggacaaaatg gctgcacaaa ctttacgcat aattacctct
     5881 cgcttttctg caataaaaaa ggcgccattt ctggcgcccg tatatgggtt ataaaattca
     5941 gctgatgctg atgcctgcgg tggctttctt catcaccaca accagcaaat cgctgatact
     6001 tgctgtggga taccagtcat ttaccagcca tgctgacacc gaaaactcca gcgtcatgtg
     6061 gccgtgacca gctggcatat caataacacc actgtaaatc agcgtattat ccagcgcggt
     6121 acggttataa atttcagcac catttttctt cactatcagg cggcatgacg aataaatatc
     6181 gttattctcc cgctcatgtt tagcgccgca gaatgccacc gccggaataa caatttgcct
     6241 gtcaaaaggc tgatcgtcat aaatcctgac ggtaatggtc cctgatggcc accgctccgg
     6301 tgcccgggag tcccgcggga aagccttacc cactgttttg acaatatcgc cttcaatctg
     6361 gttggctgac agtttccctt taatctgaca gttctcattt atcgtaacgt tgttgagcgt
     6421 cccggagttc gcattcacac tgccactgat atctgcattt ttagcggtca actttccgtc
     6481 cggtgtcagg gaaaatgccg gaggattacc gccgctggta atggtgggag ccgtcagata
     6541 tttcaggaac acttcattca tgaatatctg atcaccctga ccaacaaaca tcggttttgt
     6601 gttgccattt gcaggattaa tcatcgcaat cctgtctgcc gccagcagca cctgactctg
     6661 catgccgtcg ggggtgttct caataccggc accgataccc gcgatataaa ggcgtccgtc
     6721 ctgcatctgc tgcagcttca cagcccacat gctgttcaga ttattatttg tatcaacctg
     6781 aaccttctgt atctgctgga tcgctgcact ctggtcttcc agtttcttat tgacggtctg
     6841 tgtgatttca ttgctgacat ccgtaatgga tgtcctgatt tcagccaggt caggcgcaag
     6901 ctgaccgtta tcaatctgcg tccacagctc ctgagccaga tgggttttcc ctatctcgcc
     6961 tttgaaaaaa tccagatagc cggatgcgtc atcactcggc tgaccaacag cctccacgaa
     7021 tgccgatttg ccaacggtgt tcacactgcg aacgtaaaaa taataattat ggcccggcct
     7081 gatattgata ctggcggcta tccagtacag cgccgtgcca agatagcgtg ctgtggtttc
     7141 aacctgcctg atatccgcaa tccgcttttc cgagaaccag aactcaaact gtaccgtcgg
     7201 atcataaacc gcaagatgcg gcgtggcggt tatctgaaaa tagcccggcg tcagctcaat
     7261 ccgcgacggc gctgccggtg cggcaatccg gaacgatacc gacgccggat cgccctgctg
     7321 cccccacgca tttactgccc ggactgtcag cctgtagttc cccagagcca gttgtgtgaa
     7381 gcggtaagtg gtttccgtcg tccgggccgt gctgaccagc cgctcactgc cgtcatccgc
     7441 tgccacggtc aggcgaagca tgaaactcac gcccttcacc accttcggcg tgtcccagcg
     7501 ggccagtacc tgatactccc cgctgtctgc ggtgacttcg gcagtcaggt gatgcactgc
     7561 tggcggcgtg acaccattca ccgtgccgct ctggtcgccg tcaaagtgcg ccccgttatc
     7621 cacgatagct tctttttccg gtacatgctg cacggcggtg atggcatacg tgccgtcgtc
     7681 gttctcacgg atactcacgc agcggaacag gcgctggcgc agcgtcggca gcttcagccc
     7741 ccatacgctg tatccggcaa cgccgtcagg aacacggctc acttttacct tcacgccgtc
     7801 ggtgacggac tggacctcca cgctgaccgg attgccactt ccgtcaacca ggcttatcag
     7861 cgtggtaccg gaggatggca gcgtgatttc acggtcgagc gtcagcgtcc gggtctggct
     7921 gttcaccgcc agcacgcgcc cgccggtgct gatccccgca tagtcatcat cgcagatttc
     7981 aatgacatcg cccggcacat ggcgaagccc ttccgcaccc acgctgaagt ccacggtctg
     8041 cgtttccagc agttctgttt taatcagcca cagcccggcg cggtgcgcct gcccccggct
     8101 ggtacagcca aaggcatcca tcttcgtgac gttacgaccg taacggagaa tggcctgcgt
     8161 atcttcaaca agctctgtcg ccgtctccca gccgttatcc gggtcaatcc agttcacctc
     8221 aacggcatta tgacggtcct tcagggcact gaagctgtag cggaacggcg cgccatcatc
     8281 cggcatcacc acattactgc ggttataggt ccacacctta tccgacggtc ggtcctgcac
     8341 gaacgtcagc gtctgcccgt tccataccgg catacagcgc atcgccgagc agaaatcact
     8401 gagaacatcc cacgccttgc gctgtgtggt caggtaggca ttacaggtga tgcgcggctc
     8461 cgtgccacca aagccatccg gcaccgactg gtcgcaattc tggccgatga catacagcgc
     8521 ccatttgtcc acatccgccg caccgagacg cttccccatg ccgtagcgcg gatgggtcag
     8581 catatcccac agacaccagg ccatgttgtt gctgtatgct ggcttaaacg ttccgtccca
     8641 gataccgctg tattgccgcg tctgcgggtt atagttcgac ggcacctgca gaatgcgccc
     8701 gcgaagatga taattacggc tcacctgctg gctgccgaac tgttccgagt ccacctgcac
     8761 gccgaccagt gccgtgttcg ggtagcactg tttcacatcg atgatttcgg tgtatgacga
     8821 ccagagcgtt ttgttctgca gctggtctgt ggtgctgtcc ggcgtcattc tgcgcatccg
     8881 gatattaaac gggcgcggcg gcaggttatc caccaccacc gaggccagat actgcgaggt
     8941 ggttttgccc ttaatggtga tgtctttttc cgtcacccag ccaccattac gctgtatctg
     9001 aaccagcagg cgaacttccg acggattccg gtcccccttt gaggtggttt ccaccagtgc
     9061 ctgcacaccg aaggtaaagc gcagtcggtc gatgtttgcc gacgtgatgg cgcgggtgat
     9121 cggcgtgtcg tatttcactt ccgtacccaa caccgtctcg gagccggagg attcaaatcc
     9181 ctccggcgga gtctgctcct gctcaccagc ccggaacacc accgtgacac cggcgatgtt
     9241 ggtattccca tcactgtcca gcaccgacgt actgttcagc agcacgcttt ttaatccatc
     9301 caccggacct tcaatcggcc cttcactgat ggcatcaatc acactcagca actgcgtgga
     9361 cttcaggttg tccttcgctt cgcgcggggt atgcccctta ctgcttcctt tacccattcc
     9421 tcacgctcca taaacgacaa aaccgcccgc aggcggtttc acataaaaca ttttgcatca
     9481 gcgaccaatc accacaacct gaccaccgtc cccttcgtct gccgtgctga tctcctgaga
     9541 aaccacgcgt gaccccacgc gcatttcccc gtacagaaca ggcagaacat tgccctgggc
     9601 aaccatgtta tccagtgagg agaaataggt gttctgctta ccgttatccg ttgtctgtgt
     9661 acggggagtt ctggctttcg gtgccagcat ctgcgccaca ccaccgagca ccatactggc
     9721 accgagagaa aacaggatgc cggtcatacc accggcccca atggctgccc cccatgctgc
     9781 aagggtggct ccggcggtaa agaatgatcc ggcaatggcg gcagccccca ggacaatctg
     9841 gaatacgcca cctgacttgg ccccggcgac tctgggaaca atatgaatca cagcgccatc
     9901 aggcagagtc tcatgtaact gcgccgttaa cccggacgtg ctgacgtccc gcccggcaat
     9961 ccgtacctga taccagccgt cgctcagttt ctgacgaaac gccgggagct gtgtggccag
    10021 tgcccggatg gcttcagccc ccgttttcac acgaaggtcg atgcggcgac caaatcgttg
    10081 taaatccccg taaaggcaga tgcgcgccat gcccggtgac gccagaggga gtgtgtgcgt
    10141 cgctgccatt tgtcggtgta cctctctcgt ttgctcagtt gttcaggaat atggtgcagc
    10201 agctcgccgt cgccgcagta aattgcggcg tgattcggca ccgatgaacc aaaacagcac
    10261 agcagcacat cgcccggctg tgccgctgac aacggcacct gatacagccc tgtggcctcc
    10321 agattatcca gatagagatt ctggccgtta cgccaccagt catcctcacg atgaaaggcc
    10381 ggcatctcaa tccccgccag atgataagca tcccggaaca gtgtgtaaca gtccgtcaca
    10441 ccgtgctcaa agcgccgccc ggtgagatgc ggcacacagc ggaacttatg aatcgtcccc
    10501 cggcagacca gccaccacgg caaatcactc tgcacctgca gccgccggtc ggcctcactc
    10561 agccaggg
//
LOCUS       DAFAVF010000146        10555 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFAVF010000146 DAFAVF010000000
VERSION     DAFAVF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.146"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            146..2074
                     /gene="thrS"
                     /locus_tag="LGK42_003593"
     CDS             146..2074
                     /gene="thrS"
                     /locus_tag="LGK42_003593"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK2659800.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2078..2620
                     /gene="infC"
                     /locus_tag="LGK42_003594"
     CDS             2078..2620
                     /gene="infC"
                     /locus_tag="LGK42_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK2659801.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2717..2914
                     /gene="rpmI"
                     /locus_tag="LGK42_003595"
     CDS             2717..2914
                     /gene="rpmI"
                     /locus_tag="LGK42_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK2659802.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            2967..3323
                     /gene="rplT"
                     /locus_tag="LGK42_003596"
     CDS             2967..3323
                     /gene="rplT"
                     /locus_tag="LGK42_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK2659803.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3440..3564
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3446..3490
                     /gene="pheM"
                     /locus_tag="LGK42_003597"
     CDS             3446..3490
                     /gene="pheM"
                     /locus_tag="LGK42_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBK2659804.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3774..4757
                     /gene="pheS"
                     /locus_tag="LGK42_003598"
     CDS             3774..4757
                     /gene="pheS"
                     /locus_tag="LGK42_003598"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK2659805.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4772..7159
                     /gene="pheT"
                     /locus_tag="LGK42_003599"
     CDS             4772..7159
                     /gene="pheT"
                     /locus_tag="LGK42_003599"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK2659806.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7164..7463
                     /gene="ihfA"
                     /locus_tag="LGK42_003600"
     CDS             7164..7463
                     /gene="ihfA"
                     /locus_tag="LGK42_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBK2659807.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7564..8544
                     /gene="btuC"
                     /locus_tag="LGK42_003601"
     CDS             7564..8544
                     /gene="btuC"
                     /locus_tag="LGK42_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBK2659808.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8607..9158
                     /gene="btuE"
                     /locus_tag="LGK42_003602"
     CDS             8607..9158
                     /gene="btuE"
                     /locus_tag="LGK42_003602"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBK2659809.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9158..9907
                     /gene="btuD"
                     /locus_tag="LGK42_003603"
     CDS             9158..9907
                     /gene="btuD"
                     /locus_tag="LGK42_003603"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBK2659810.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            9985..10449
                     /locus_tag="LGK42_003604"
     CDS             9985..10449
                     /locus_tag="LGK42_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK2659811.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2633 a         2500 c         2877 g         2545 t
ORIGIN      
        1 aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga
       61 tggtttcgca atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg
      121 tcaccactgc aaataaggat ataaaatgcc tgttataact cttcctgatg gcagccaacg
      181 ccattacgac cacgctgtaa gccccatgga tgttgcgctg gacattggtc caggtctggc
      241 gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt gatgcttgcg atctgattga
      301 aaacgacgca caactgtcca tcattaccgc caaagacgaa gacggtctgg agatcattcg
      361 tcactcctgt gcgcacctgt tagggcacgc gattaaacaa ctttggccgc ataccaaaat
      421 ggcaatcggc ccggttattg acaacggttt ttattacgac gttgatcttg accgcacgtt
      481 aacccaggaa gatgtcgaag cactcgagaa gcggatgcat gagcttgctg agaaaaacta
      541 cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt gaaacattcg ccaaccgtgg
      601 ggagagctac aaagtctcca ttcttgacga aaacatcgcc catgatgaca agccaggtct
      661 gtacttccat gaagaatatg tcgatatgtg ccgcggtccg cacgtaccga acatgcgttt
      721 ctgccatcat ttcaaactaa tgaaaacggc aggggcttac tggcgtggcg acagcaacaa
      781 caaaatgttg caacgtattt acggtacggc gtgggcagac aaaaaagcgc ttaacgctta
      841 cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt aaaatcggta aacagctcga
      901 cctgtaccat atgcaggaag aagcgccggg tatggtattc tggcacaacg acggctggac
      961 catcttccgt gaactggaag tgtttgttcg ttctaaactg aaagagtacc agtatcagga
     1021 agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa aaaaccggtc actgggacaa
     1081 ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt gaatactgca ttaagccgat
     1141 gaactgcccg ggtcacgtac aaattttcaa ccaggggctg aagtcttatc gcgatctgcc
     1201 gctgcgtatg gccgagtttg gtagctgcca ccgtaatgag ccgtcaggtt cgctgcatgg
     1261 cctgatgcgc gtgcgtggat ttacccagga tgacgcgcat atcttctgta ctgaagaaca
     1321 aattcgcgat gaagttaacg gatgtatccg tttagtctat gatatgtaca gcacttttgg
     1381 cttcgagaag atcgtcgtca aactctccac tcgtcctgaa aaacgtattg gcagcgacga
     1441 aatgtgggat cgtgctgagg cggacctggc ggttgcgctg gaagaaaaca acatcccgtt
     1501 tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa attgaattta ccctgtatga
     1561 ctgcctcgat cgtgcatggc agtgcggtac agtacagctg gacttctctt tgccttctcg
     1621 tctgagcgct tcttatgtag gcgaagacaa tgaacgtaaa gtaccggtaa tgattcaccg
     1681 cgcaattttg gggtcgatgg aacgtttcat cggtatcctg accgaagaat tcgctggttt
     1741 cttcccgacc tggcttgcgc cagttcaggt tgttatcatg aatattaccg attcacagtc
     1801 tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg ggcattcgtg ttaaagcaga
     1861 cttgagaaat gagaagattg gctttaaaat ccgcgagcac actttgcgtc gcgtcccata
     1921 tatgctggtc tgtggtgata aagaggtgga atcaggcaaa gttgccgttc gcacccgccg
     1981 tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc gagaagctgc aacaagagat
     2041 tcgcagccgc agtcttaaac aattggagga ataaggtatt aaaggcggaa aacgagttca
     2101 aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc caggaagttc gcttaacagg
     2161 tctggaaggc gagcagcttg gtattgtgag tctgagagaa gctctggaga aagcagaaga
     2221 agccggagta gacttagtcg agatcagccc taacgccgag ccgccggttt gtcgtataat
     2281 ggattacggc aaattcctct atgaaaagag caagtcttct aaggaacaga agaaaaagca
     2341 aaaagttatc caggttaagg aaattaaatt ccgtcctggt acagatgaag gcgactatca
     2401 ggtaaaactc cgcagcctga ttcgctttct cgaagagggt gataaagcca aaatcacgct
     2461 gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt atggaagtgc ttaatcgcgt
     2521 gaaagacgat ttgcaagaac tggcagtggt cgaatccttc ccaacgaaga tcgaaggccg
     2581 ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa ggccttcaag tagcactgtc
     2641 tgtggagcct tcgggcttca caggctttgt tcgcctttgt ttcgtttatt aacaatgcga
     2701 agtggaagtt attaaaatgc caaaaattaa gaccgtacgc ggtgctgcta agcgcttcaa
     2761 aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac ctgcgtcaca ttctgaccaa
     2821 aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc atggtttcca aaggcgatct
     2881 gggcctggta atcgcgtgcc tgccgtacgc ataagccgtt aacgttttta actttttaat
     2941 tagaatatag atacaggaga gcacatatgg ctcgcgtaaa acgtggtgtt attgcacgcg
     3001 cacgtcacaa gaaaattttg aaacaagcta aaggctacta cggtgcgcgt tctcgcgtat
     3061 accgcgttgc cttccaggct gttatcaaag ctggtcagta tgcttaccgt gaccgtcgtc
     3121 aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat caacgcagca gcacgtcaga
     3181 acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa agcctctgtt gaaatcgacc
     3241 gtaagatcct ggctgatatc gcagtattcg acaaagtagc gttcaccgct ctggttgaaa
     3301 aagcgaaagc agctctggca taagccagtt gaaagaggga gctagtctcc ctcttttcgt
     3361 ttcaacgcca tcaaaacatt gacttttatc gccgtagcct tttcaataaa ggtcttttga
     3421 agagtaccaa aaggtaacgc aagcaatgaa tgctgctatt ttccgcttct ttttttactt
     3481 tagcacctga atccaggagg ctagcgcgtg agaagagaaa cggaaaacag cgcctgaaag
     3541 cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa ataaaaagcc tgtctcatca
     3601 ggtgtaattg tcgcagccag tctggacacg gccagcgcgg agaaaccgga gcgtatttaa
     3661 gtacgtgaga atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg
     3721 ataggctcta agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac
     3781 atctcgcaga actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg
     3841 cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga
     3901 cgaccctgcg tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag
     3961 cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgcagcgc
     4021 tgaatgcgcg tctggcggcg gaaacgattg atgtctccct gccaggtcgt cgcattgaaa
     4081 acggcggtct gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc
     4141 ttggctttac cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc
     4201 tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacacta
     4261 cccgcctgct gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc
     4321 caccgattcg tatcatcgcg cctggccgcg tttatcgtaa cgactacgac cagactcaca
     4381 cgccgatgtt ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc
     4441 tgaaaggcac tctgcacgat ttcctgcgta acttctttga ggaagatttg cagattcgct
     4501 tccgtccttc ctacttcccg ttcaccgagc cttctgcaga agttgacgtc atgggtaaaa
     4561 acggtaaatg gctggaagtg ctgggttgcg ggatggtgca tccgaacgtg ctgcgtaacg
     4621 ttggcatcga cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga
     4681 ctatgttgcg ttacggcgtc accgacctgc gttcattctt cgaaaatgat ctgcgtttcc
     4741 tcaaacagtt taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga
     4801 atgggtgaac ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct
     4861 ggaagttgac ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt
     4921 ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgttgg
     4981 cggcgatcgc ctgctggaca tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt
     5041 ggcggtagct accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact
     5101 gcgtggtgaa ccgtctgaag ggatgctgtg ctccttttct gagctgggaa tttctgacga
     5161 tcatagcggc attatcgagc tgcctgcgga tgcgccgatt ggcaccgaca tccgtgaata
     5221 cctgaaactt gatgacaaca ccatcgaaat cagcgtgacg ccaaaccgtg ccgactgttt
     5281 aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc
     5341 ggaaatcgtt ccggttggtg cgaccatcga cgacacgctg ccgattacag tcgaagcgcc
     5401 ggaagcctgc ccgcgttatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac
     5461 tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt
     5521 tgacgtcacc aactatgtac tgctcgaact gggccagccg atgcacgctt tcgataaaga
     5581 tcgcattgaa ggcggcattg tggtgcggat ggcgaaagag ggcgaaacgt tggtactgct
     5641 tgatggcact gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc
     5701 gctggcgatg ggcggcatct ttggtggcga acactctggc gtgaatgacg aaacgcaaaa
     5761 cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca
     5821 tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagca
     5881 caaagcgatg gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc
     5941 ggtaattgat atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg
     6001 tagcaaactg gatcgcctga tcggccatca tattgcggat gagcaggtaa ctgacattct
     6061 gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag
     6121 ctggcgtttc gatatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg
     6181 ctacaacaac atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga
     6241 agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt
     6301 gatcacctac agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc
     6361 cttactgctg ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac
     6421 cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt
     6481 cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct
     6541 gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga
     6601 gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa
     6661 actgaatgag gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc
     6721 agcgatttat ctgaaaggtg aacgtattgg ttttgttggg gttgttcatc ctgaactgga
     6781 acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc
     6841 agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat
     6901 cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt
     6961 tggcgtaaat caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc
     7021 ggaggggtat aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga
     7081 agaagaggag attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca
     7141 ggcatcattg agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt
     7201 ttgataagct tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag
     7261 agatccgtcg cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg
     7321 atctgcgtga taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca
     7381 ttacagcacg gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa
     7441 acgcttcgcc caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt
     7501 tcttttcact gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat
     7561 accatgctga cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg
     7621 tcagttttga tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatctcg
     7681 ccaggtgact ggttttctcc tcgtggcgaa ctgttcgtct ggcagattcg cctgccacgt
     7741 acgctggctg tattgctggt tggtgcggcg ctggctatat ccggcgcagt aatgcaggcg
     7801 ttgtttgaaa atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgta
     7861 gggcttatcg ccgcggtatt gcttgggcaa gggcaactcc caaactgggc actagggctg
     7921 tgtgcgattg ctggcgcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat
     7981 ctttcgacca gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta
     8041 atgacgtggg ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg
     8101 atgggcggtt ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc
     8161 gtgttgttgt ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc
     8221 tcggcgcggc aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc
     8281 ggctggatgg ttggcgtcag tgtggcgctg gcgggtgcta tcggctttat tggtctggtg
     8341 atcccacata ttcttcggtt gtgtggttta accgatcatc gcgtattact tcccggctgc
     8401 gcgctggcag gggcgagcgc attgctgctg gccgatgttg tagcgcgctt ggcattagct
     8461 gccgcagagc tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg
     8521 ttattgttaa aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt
     8581 tttactgacc acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag
     8641 atatcgacgg tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca
     8701 atgtcgcctc aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag
     8761 cctgggccga tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag
     8821 aaccgggcag cgatgaagag attaaaactt actgtaccac cacatggggg gtgacgttcc
     8881 cgatgttcag taagattgaa gttaatggcg aaggacgcca tccgctgtat caaaaattga
     8941 ttgccgcagc gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca
     9001 gcaaaggtcg tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag
     9061 ttggcaggga cggattggtc atccagcgtt tttccccgga tatgacgccg gaagacccca
     9121 ttgtgatgga aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca
     9181 ggatgttgcg gaatctaccc gcctggggcc actttctggc gaggttcggg ctggggagat
     9241 cctgcatctg gtggggccga atggcgcggg taagagtacc ttgcttgcac gaatggccgg
     9301 aatgaccagc ggtaagggaa gcattcagtt cgcggggcaa cccctggaag catggtccgc
     9361 aacaaaactc gcgctgcatc gcgcctatct ttcacaacag cagacgccgc cgtttgcaat
     9421 gccggtctgg cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa
     9481 tgatgtcgca ggggcgctgg ctcttgatga caaactcgga cgtagcacca atcaactttc
     9541 cggcggtgaa tggcaacgcg tacgtcttgc tgcggtggtg ctgcaaatca caccacaagc
     9601 caatcccgca ggtcaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca
     9661 acaaagtgcg ttagacaaaa ttctgagcgc tctgtgtcag caaggactgg cgattgtgat
     9721 gagcagtcac gatctcaacc acacattgcg tcatgcgcat cgggcgtggt tgctaaaagg
     9781 tggaaaaatg ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca
     9841 ggcctatggg atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac
     9901 catctgattt cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa
     9961 taaaaaacag aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt
    10021 ctggccgggt gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt
    10081 accgttattg ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat
    10141 ggtggcatga cgcggcgcgg tgtggactgt tcgggatttg tggttgtaac gatgcgcgat
    10201 cgtttcgatt tgcagctgcc ccgagaaacc aaagaacaag cctctatcgg cacgcaaatt
    10261 gataaagacg agttgctgcc tggtgacctg gtctttttca aaacggggtc cggacaaaat
    10321 ggtttgcatg taggtattta cgataccaac aaccaattta tccacgcctc taccagcaag
    10381 ggagtgatgc gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga
    10441 cgaatctagt atgtcgaaaa atggacggcg atgatacatc gccgtccatt tactcctgaa
    10501 tacagtagta ttttgcagac aaaaattccg taaatggatt attcgtttat aatca
//
LOCUS       DAFAVF010000147        10283 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFAVF010000147 DAFAVF010000000
VERSION     DAFAVF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.147"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            1..1439
                     /locus_tag="LGK42_003605"
                     /pseudo
     CDS             1..1439
                     /locus_tag="LGK42_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
     gene            1662..2159
                     /gene="ubiC"
                     /locus_tag="LGK42_003606"
     CDS             1662..2159
                     /gene="ubiC"
                     /locus_tag="LGK42_003606"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBK2659812.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2172..3044
                     /gene="ubiA"
                     /locus_tag="LGK42_003607"
     CDS             2172..3044
                     /gene="ubiA"
                     /locus_tag="LGK42_003607"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBK2659813.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3199..5622)
                     /gene="plsB"
                     /locus_tag="LGK42_003608"
     CDS             complement(3199..5622)
                     /gene="plsB"
                     /locus_tag="LGK42_003608"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBK2659814.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5793..6161
                     /gene="dgkA"
                     /locus_tag="LGK42_003609"
     CDS             5793..6161
                     /gene="dgkA"
                     /locus_tag="LGK42_003609"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK2659815.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6271..6879
                     /gene="lexA"
                     /locus_tag="LGK42_003610"
     CDS             6271..6879
                     /gene="lexA"
                     /locus_tag="LGK42_003610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBK2659816.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6952..8277
                     /gene="dinF"
                     /locus_tag="LGK42_003611"
     CDS             6952..8277
                     /gene="dinF"
                     /locus_tag="LGK42_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBK2659817.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            8393..8602
                     /locus_tag="LGK42_003612"
     CDS             8393..8602
                     /locus_tag="LGK42_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK2659818.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8644..9159)
                     /gene="zur"
                     /locus_tag="LGK42_003613"
     CDS             complement(8644..9159)
                     /gene="zur"
                     /locus_tag="LGK42_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBK2659819.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            9477..10283
                     /locus_tag="LGK42_003614"
                     /pseudo
     CDS             9477..10283
                     /locus_tag="LGK42_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2581 a         2297 c         2627 g         2778 t
ORIGIN      
        1 tcagcaatat tttttcaaat ataaaacaac ggaacgaaaa aatcatgcaa taaatatgtt
       61 tcgtgaagtc tcaatcagta atgatactat aagtgtaaaa ttctacagaa atgaaaaaaa
      121 ttgaatgcgc ttgcaatttt ctgatggata aagatgcgca ggggtatatc gacctgtctg
      181 atttggattt aacaagttgt cattttaaag gtgacgttat atcgaaggtg tcttttttat
      241 catcaaatct acaacatgta acattcgaat gtaaagaaat tggggattgc aattttacta
      301 ctgcaatagt tgataatgtc atatttagat gtcgacgttt acacaatgtg atttttatca
      361 aagcgagtgg tgaatgtgtc gatttcagca aaaatattct tgatacagtt gacttctcgc
      421 agagtcaact tactcagagt aattttcgcg aatatcagat tagaaattca aacttcgata
      481 attgttatct ttacgcttcg cacttcacca gagcagagtt tctgtctgcc aaagaaatat
      541 catttattaa atcgaatatg acagctgtta tgtttgatca tgtgcgaata tcgacaggga
      601 attttaaaga ttgcattaca gaacaattgg aattaactat tgattattca gatatatttg
      661 ggaatgaaga tctcgatggt tatatcaata acattataaa aatgattgat acattgccag
      721 ataatgcaat gatattgaaa tccgttctgg cagtaaaact ggttatgcaa ttaaaaattc
      781 ttaatattgt taataaaaac tttattgaga atatgaagaa aacatttagc cattgtcctt
      841 atataaaaga tccaattata cgcagttata tccattctgg tgaagataac aagttcgatg
      901 attttatgcg tcaacatcga ttcagcaagg tggatttcga tacccaacag atgatccatt
      961 ttattaacag gtttaatatg aataaagggc tgattgataa aaataacaat ttttttatcc
     1021 aacttatcga tcaggcctta cgatcaacgg atgatatgat caaagcaaat gcctggtatc
     1081 tttataaaga gtggattcgt agtgatgatg tttcacctat atttatagaa actgaagaaa
     1141 agttaagaac ctttaacact aataaattaa cacgaaacga taatatattt atcctgttct
     1201 cctctgtcga tgatgggcct gttatggtgg taagctccca gcgcttacat gatatgttga
     1261 atcctacaaa agataccaat tggaattcca cgtatatcta caaatccaga catgagatgt
     1321 tgcctgttaa tcttactcag gaaacacttt tcagctccaa atctcatggt aaatatgcgc
     1381 ttttccccat ttttactgcg agttggcgag ctcatcgtat aatgaataag ggtgtttaag
     1441 taaaggaaaa catcaccgtt cctggcatcc tgggcggtga tgcccctatg gttgccctct
     1501 ccagcgcggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt
     1561 tgcgtttttg ttgcgtatta gatcacttaa tttgctttac atctcccgta aacacttttc
     1621 tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa
     1681 cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggaaccg caactgctcg
     1741 actggctgtt gctggaggat tccatgacaa aacgttttga acagcaggga aaaacggtaa
     1801 gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa gaactgccgc
     1861 tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac
     1921 cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt
     1981 tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt cacatcatcg acattaaccc
     2041 gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat
     2101 taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag
     2161 aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa
     2221 tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt
     2281 gggtggcgac accgggcgtt cctcagctct ggatcctggc ggtatttgtc gcgggtgtct
     2341 ggctgatgcg cgctgctgga tgtgtggtga atgattatgc tgaccgaaag tttgatggtc
     2401 atgttaagcg cacggcgaac cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc
     2461 gcgcgctgtt tgtagtgctg gtactgattt cgtttttact ggtactgacg ctgaatacga
     2521 tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc
     2581 ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg
     2641 cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc ctcgccaata
     2701 ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat gatgatgtga
     2761 agattggcat taaatccacg gcaatcctgt tcggccaata cgataaattg attattggta
     2821 ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat ggcttaggct
     2881 ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga
     2941 ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc
     3001 tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg
     3061 gatgatcatc cggctttctt ctgggttgcc ttatgcgcgg cgcttctcag gcctacacaa
     3121 cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt tttcgccgca
     3181 tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac
     3241 gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg ccggttcggc
     3301 gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag aactgaacac
     3361 cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac gttgcgcgac
     3421 ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac tcaacaacca
     3481 gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagggt
     3541 gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg
     3601 ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat cccagcgcag
     3661 gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg
     3721 ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg
     3781 cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca gaatgatgat
     3841 gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag
     3901 ctcgctggcg ctggctgaag gaacggtaga gtctgtggag taaggtacgt tgcgcatcag
     3961 atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga
     4021 tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg
     4081 taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg gacgcaccgc
     4141 ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa
     4201 cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc
     4261 gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta
     4321 agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg taatcggacg
     4381 cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag
     4441 caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat aaccacggct
     4501 gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc ctttaaaagt
     4561 acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaagttcag
     4621 gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca cgtaagaaag
     4681 tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat gaccgtcgtg
     4741 ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa gtcggttcca
     4801 ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat tcgccgcaat
     4861 ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg
     4921 cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat taaacagatc
     4981 ctgacgagca ggcagacgtg ggcccacagc agccagacgt tgacgggcaa agtgcatacg
     5041 cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat ccgccatacg
     5101 gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacggc
     5161 gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc ctttttcacg
     5221 ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt
     5281 gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt tcggcgtgta
     5341 ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc
     5401 gatttccagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg cgcgtaacgt
     5461 cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag aagtatccag
     5521 ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg
     5581 taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct
     5641 ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc
     5701 cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat
     5761 attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc
     5821 gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag
     5881 cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg
     5941 atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg
     6001 aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa taccatgagc
     6061 tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgccatt atcgtcgccg
     6121 tgattacctg gtgcattctg ttatggtcgc attttggata acccttccag atttcgataa
     6181 atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta tatactcaca
     6241 gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag gcaacaagag
     6301 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     6361 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     6421 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
     6481 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg
     6541 caacagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc gaatgctgat
     6601 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggtgacttg
     6661 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     6721 gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa
     6781 aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac cattgaaggg
     6841 ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga gaccgcgatg
     6901 ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg catggcattc
     6961 ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt ctccaatatc
     7021 accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga tagcccggtt
     7081 tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat gctgttgctg
     7141 tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa aaatcctcag
     7201 gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg ggcgttaatt
     7261 gcgctgctgc gtacaccgat tatcgatctg gcgctgcata ttgttggcgg tagtgaagcg
     7321 gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgctcc ggcgtcgctg
     7381 gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt
     7441 ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt gatggggctg
     7501 catatgaacg tgcagggcgc ggcgctggcg acggttattg cggaatatgc aacattgctg
     7561 attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg cgaaatgctg
     7621 aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg
     7681 cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc gcgactgggg
     7741 agtgacattg tcgctgttaa cgcggttctg atgacgctac tcacctttac cgcctatgcg
     7801 cttgatggtt ttgcctacgc ggttgaagcg cactccggtc aggcatacgg tgcgcgcgac
     7861 ggtagccagt tgctggatgt atggcgggca gcgtgccgcc agtcggggat tgtagcgtta
     7921 ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct gacgtcgtta
     7981 acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt gccgttggtt
     8041 ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc cgccgaaatg
     8101 cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac gctgccgtgg
     8161 ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg cgggctttct
     8221 ctggcggcta tctggcggcg tcactggcgc aacggtacct ggtttgccgc aacgtgacgg
     8281 ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa tctcacgttc
     8341 agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga ttatgaataa
     8401 agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa
     8461 actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg tcggtaaaat
     8521 ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac
     8581 ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag gatgtacgcc
     8641 ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact gttcaggatg
     8701 acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa tcacattatg
     8761 ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca cgccttctgc
     8821 acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg tatgcgtggg
     8881 ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat gcacaaaacc
     8941 ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt gcggttcagc
     9001 ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca gactcatcag
     9061 gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc agattttttc
     9121 agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca aagtgggggt
     9181 taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt attgttgccg
     9241 taatgttgat tttctgtttt gtaggtaagg tgttgtgttg ccttgtcgta ccattatcaa
     9301 cacgataata attaataatt catttttaaa taaattgctt caagttaact cgctatttat
     9361 ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt tgatagtgac
     9421 cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga tgaatcatgt
     9481 tgaagataat acccggtgca actggatact ttaataaaac actcaattca aatcaattcg
     9541 ataatgaaga tgcaatcaaa gataaattag ataatagagg ttcaatcaaa ggtaaattaa
     9601 ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat ataattattg
     9661 caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca cgtaaaaaaa
     9721 tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt atattgacga
     9781 ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg accgggctat
     9841 ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg cgactcccat
     9901 tgaacgatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg ttcagctatt
     9961 ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag caataggttc
    10021 tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct taaaattatt
    10081 tgcttcgctg ttaatttcaa tttcatattc tagtgcctac acagatttag ccggagcaga
    10141 aaaagtgaat attaatgaat ataatgagaa ctatctgaca gctcagtttg aagaattatc
    10201 tcaacgtgat atgaagaagt acctgggaga gatgaagcat ctggcggacc gggggggatg
    10261 aaatttgatg actatctgga taa
//
LOCUS       DAFAVF010000148        10269 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFAVF010000148 DAFAVF010000000
VERSION     DAFAVF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.148"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            147..689
                     /locus_tag="LGK42_003615"
     CDS             147..689
                     /locus_tag="LGK42_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659820.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            789..1991
                     /locus_tag="LGK42_003616"
     CDS             789..1991
                     /locus_tag="LGK42_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBK2659821.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2088..2516
                     /locus_tag="LGK42_003617"
     CDS             2088..2516
                     /locus_tag="LGK42_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBK2659822.1"
                     /translation="MARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDEILDVEG
                     IDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCL
                     AWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2557..3459)
                     /locus_tag="LGK42_003618"
     CDS             complement(2557..3459)
                     /locus_tag="LGK42_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBK2659823.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETAGDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3653..4843)
                     /gene="glpC"
                     /locus_tag="LGK42_003619"
     CDS             complement(3653..4843)
                     /gene="glpC"
                     /locus_tag="LGK42_003619"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBK2659824.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4840..6099)
                     /gene="glpB"
                     /locus_tag="LGK42_003620"
     CDS             complement(4840..6099)
                     /gene="glpB"
                     /locus_tag="LGK42_003620"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBK2659825.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6089..7717)
                     /gene="glpA"
                     /locus_tag="LGK42_003621"
     CDS             complement(6089..7717)
                     /gene="glpA"
                     /locus_tag="LGK42_003621"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBK2659826.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7990..9348
                     /gene="glpT"
                     /locus_tag="LGK42_003622"
     CDS             7990..9348
                     /gene="glpT"
                     /locus_tag="LGK42_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBK2659827.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9353..>10269
                     /gene="glpQ"
                     /locus_tag="LGK42_003623"
     CDS             9353..>10269
                     /gene="glpQ"
                     /locus_tag="LGK42_003623"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK2659828.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQ"
BASE COUNT         2330 a         2569 c         2849 g         2521 t
ORIGIN      
        1 tattttttcg gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct
       61 tttttcgctc attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg
      121 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
      181 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
      241 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
      301 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
      361 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
      421 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
      481 gtttgtttgg cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg
      541 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
      601 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
      661 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
      721 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
      781 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
      841 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat
      901 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
      961 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
     1021 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcatggt gctgcatgaa gcctggctca
     1081 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     1141 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     1201 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     1261 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     1321 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     1381 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcttca atgccgatta
     1441 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     1501 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     1561 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     1621 gcggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     1681 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     1741 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     1801 cgctagccac gccagacggc actttcgctc tgcgtgtgcg tttcagcact acacgctaca
     1861 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     1921 taaacggcca ggacatcgcc agtgagcatg gctggattga ggttgttgag tccatgacct
     1981 tatctgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     2041 aaaacttgtc caaccctccc taaggtgagc gtggtgttgg ggccagtgtg gcacgggctg
     2101 cgcgctgggg acgcattgag aattacatgg cgcaagttaa cgattcactt tgtctgttgg
     2161 tgcaggtgga aagtaaaacg gcactggata acctggacga gatcctcgac gtcgaaggga
     2221 ttgatggcgt gtttattgga ccagcggatc tttctgcgtc gttgggctac ccggataacg
     2281 ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc cgtgctgcgg
     2341 gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc ctggcgtggg
     2401 gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc ctggatcaac
     2461 gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt tattgatatc
     2521 aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg ctgccagatc
     2581 ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga tcaatgccct gttccagcat
     2641 gcgcaaagca attttaatgg cttgttcatg cataccttcc tgccagccaa tttgatgccc
     2701 ttcctgccgt agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc
     2761 ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt
     2821 aaacagtgtt tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct
     2881 gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt ccagcagagc
     2941 tatacgccga tgttgcatga tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa
     3001 agcttcggtg tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttaag
     3061 tgagtacggg tagggcgatg tttcgccatg ataaaacagc aatggcacca ccagcggaac
     3121 tctgtcatag cctttatcca ggtgacgctg catggcggca gtggcatagc gcattagccg
     3181 aaaagccata ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccgtc
     3241 accgtcgctg gtttccacgg accacaaaac atccgagtag taagcgcgta aacttttttc
     3301 aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc
     3361 tggtaaatgt atttcgagaa aatcccctgc ggtttcgggt gtgaacataa aggttttaaa
     3421 caccgcatca tgcggggagg atgttgttga ttcggtcatg gttgtccgtc acctgatatt
     3481 tatgatgacg cgacattacg tctatcccgg gcctttgaca tctgtcagtt tcagcctttg
     3541 cgagacattt tcagaggaat tttcaaagcg ttgcaacgca tacatggtgc gtggaatcgc
     3601 gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca tcaaaaggtc ggttaagcca
     3661 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag gctcgtggac atctcaatct
     3721 gccatttgca ggtttcgcag tcggtgatga ccagatccgc gccgctctct tctatctggc
     3781 ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     3841 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggattttac
     3901 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3961 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     4021 acagccagcg ggttgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     4081 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     4141 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     4201 ctttatcggt aaagccgttg gcgattagcg gtacgccgca gcatttttct ttgctgagca
     4261 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     4321 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatat tgtgcttgct
     4381 gagccgctat gctgcgatac cagcgacgga acgtgccgaa ggagtatttc ggtagcgtgc
     4441 ggcgatgatc gatttttaac gccgcatcaa gcagctgccg caccggtttc agcgaggtag
     4501 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatgactca
     4561 acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt tgcccgcgcg cgctggataa
     4621 tatcgccaat cttcacatcg gacgggcagg cgacttcaca acgtttgcag ttgatgcaat
     4681 atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg cagacgctcg ccatccggcc
     4741 cggcttgttt tggccctgga taaccgggat tcacccggct caccgggcag gcggtggtgc
     4801 agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt cattgttggc ctcctgcgcg
     4861 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4921 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt
     4981 ttgccctgcc tgcgacgggc gtagcgtctc atcagtggtt acaccgaact gctgccacgg
     5041 ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     5101 aaggccgaga atcggctcgc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     5161 actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga atatcggcgt gattgcgggt
     5221 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     5281 ccacacgcca ccctggcgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     5341 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     5401 acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     5461 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     5521 acgggcgata ttcacagcgc gaaattcggt ggcgttattg cgcaggacat ccagttccgg
     5581 cagctctatt tctgcggttt caaccgaaag atccagttcg cgcaacgaag ccgccgcaag
     5641 atgcgcctgg aaatccatca gcccgcttat ccccactaca catatcttct tcgcgggtag
     5701 cggccagacg ggaacttctg gcgaacttaa ccaggttgca cgcaaagtgc cgagcggggt
     5761 aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc gcaccgcttt cagcgatcaa
     5821 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt tcgagaagac tgtaaggatg
     5881 ggctggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca ccggttgacc
     5941 atcaggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     6001 acgcgtgaca atggcacagc gcaggccgtg tttttgcagt tgcaggccac agagtaatcc
     6061 ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc tttctgctcc ttctccagac
     6121 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgcg tctccccagg
     6181 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     6241 gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc agcgcggcag gcgcagagtt
     6301 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     6361 cgtttaaatt ttctaccgcg tactgaactt cacccgcagt taccgcttcg cactcacata
     6421 ccaggctacg atgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     6481 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     6541 cgggttcttg tgaaccaggc agtgccagat cggcagtcgt acaggggcgc gtgttgccca
     6601 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     6661 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     6721 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     6781 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     6841 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6901 tacgtaaaga ggtggtacca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6961 cggaaggttt acggcagcgg ttgatcacat gctggttaat gcggtgatcc atgatcagca
     7021 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     7081 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     7141 gatgattacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     7201 catgagcggt aaggataacg gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     7261 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     7321 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     7381 cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc atcttccggc agggtgataa
     7441 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     7501 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     7561 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     7621 gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc cgttgcgccg ccaccaatga
     7681 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     7741 tttaccattt agccatagta aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa
     7801 ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat tttgtaaatc ttatttaata
     7861 atgtgtgcgg caattcacat ttaatttctg aatgttttct taacatcgcg gcaactaaag
     7921 aaacggcagg ttctcccact gaatcagtct gttaatcata aataagacca cgggccacgg
     7981 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgctgcg
     8041 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     8101 tatgcggctt actatttggt tcgtaagaac tttgcgcttg ctatgcctta tctggttgag
     8161 cagggattct cacgcggtga tttaggtttt gccctttcgg ggatctcgat tgcttatgga
     8221 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     8281 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     8341 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     8401 tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     8461 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     8521 ctggggatgg cctggttcaa tgactggcat gcggcgctct atatgcctgc tttctgcgcc
     8581 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     8641 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     8701 gagctgacgg cgaagcaaat cttcatgcag tacgtactgc cgaacaaact gctgtggtat
     8761 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     8821 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcctt
     8881 tatgaatatg caggtattcc gggcactctg ctgtgcggct ggatgtcgga taaagtcttc
     8941 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     9001 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     9061 ggcttcctga tctacggtcc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     9121 aaaaaagcgg caggtacggc agcgggcttt accgggctgt ttggttacct gggcggttcg
     9181 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     9241 atggtaatga ttggcggcag cattctggcg gttatcttgt tgattgttgt gatgattggc
     9301 gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     9361 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     9421 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     9481 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     9541 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attatctcga
     9601 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     9661 cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat
     9721 tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc ccaatgggta agtccgactt
     9781 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     9841 gaaaaatatc ggtatttatc ctgaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9901 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaggacga
     9961 caaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
    10021 acctaaaatg ggcatggatc tcaatctggt acagttgatt gcctataccg actggaatga
    10081 aacgcagcag aaacagccgg atggaagctg ggttaattac aactacgact ggatgtttaa
    10141 gccgggtgct atgaaacagg tggcggaata tgccgacggt atcgggccgg attaccatat
    10201 gttgattgag gaaacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
    10261 tcagcagaa
//
LOCUS       DAFAVF010000149        10236 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFAVF010000149 DAFAVF010000000
VERSION     DAFAVF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.149"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            78..653
                     /gene="ssuE"
                     /locus_tag="LGK42_003624"
     CDS             78..653
                     /gene="ssuE"
                     /locus_tag="LGK42_003624"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBK2659829.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            646..1605
                     /gene="ssuA"
                     /locus_tag="LGK42_003625"
     CDS             646..1605
                     /gene="ssuA"
                     /locus_tag="LGK42_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBK2659830.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1602..2747
                     /gene="ssuD"
                     /locus_tag="LGK42_003626"
     CDS             1602..2747
                     /gene="ssuD"
                     /locus_tag="LGK42_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBK2659831.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2758..3549
                     /gene="ssuC"
                     /locus_tag="LGK42_003627"
     CDS             2758..3549
                     /gene="ssuC"
                     /locus_tag="LGK42_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBK2659832.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3546..4313
                     /gene="ssuB"
                     /locus_tag="LGK42_003628"
     CDS             3546..4313
                     /gene="ssuB"
                     /locus_tag="LGK42_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBK2659833.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4520..7132)
                     /gene="pepN"
                     /locus_tag="LGK42_003629"
     CDS             complement(4520..7132)
                     /gene="pepN"
                     /locus_tag="LGK42_003629"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBK2659834.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7398..8600
                     /gene="pncB"
                     /locus_tag="LGK42_003630"
     CDS             7398..8600
                     /gene="pncB"
                     /locus_tag="LGK42_003630"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK2659835.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8769..10169
                     /gene="asnS"
                     /locus_tag="LGK42_003631"
     CDS             8769..10169
                     /gene="asnS"
                     /locus_tag="LGK42_003631"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK2659836.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2293 a         2662 c         2777 g         2504 t
ORIGIN      
        1 ttcttgtctc tccgacagac tgaccgaatt gttatcaatg ttaacaaaaa aagaacaatt
       61 ggttataagg agagagtatg cgtgtcatca ccctggcggg tagtcctcgc tttccttctc
      121 gctccagctc cttgctggaa tatgcgcggg aaaaactaaa tggcctcgat gtagaggttt
      181 atcactggaa tctgcaaaac ttcgccccgg aagatctgct ttatgctcgt ttcgatagtc
      241 cggcactcaa gaccttcacc gaacagctgc aacaggctga tggactgata gtcgctacac
      301 ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct gctcgacctg ctgccagagc
      361 gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg cggtaccgtg gcccatctgc
      421 tggcggtcga ttatgccctt aaaccagttt taagcgcact gaaagcccag gagatcctgc
      481 acggcgtgtt tgccgatgac tcacaagtca ttgattacca tcacagaccc cagttcacgc
      541 caaatctgca aacccgtctt gataccgcgc tagaaacttt ctggcaggca ttgcaccgcc
      601 gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa tgtccatgcg taacatcatt
      661 aaactggcgc tggcgggatt gcttagcgtc tctacttttg cggttgctgc agaatcctcg
      721 cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg gtatggtgct ggcaaaaagc
      781 caccagttac tggaaaaacg ctatccgcaa tcaaaaatct cctgggtgga gttccccgcg
      841 ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg atctcggcag taccggggat
      901 attccgccaa tctttgccca ggctgccggg gctgatttgg tatatgtggg cgtcgagcca
      961 ccgaagccca aagccgaagt gattctggtg gcagaaaaca gcccgatcaa aaccgtagcc
     1021 gatcttaaag gtcacaaagt tgcctttcag aaaggttcca gttcacacaa ccttttactg
     1081 cgtgcactgc gtcaggccgg acttaagttt accgatatac aacccactta cctgacacct
     1141 gccgatgccc gcgccgcgtt ccagcaaggt aacgttgacg cctgggctat ctgggatccc
     1201 tactactccg ctgcgttatt acagggcggc gtgcgggtgt tgaaagacgg caccgatctc
     1261 aatcaaaccg gatcgtttta tctggcagct cgcccctatg cagaaaaaaa cggcgctttt
     1321 attcagggcg tactggcaac ctttagtgag gccgatgcgt taacccgcag ccagcgcgag
     1381 caaagcatcg ctttactggc aaaaacgatg ggcttaccgg caccggtgat tgcctcttac
     1441 ttagatcatc gccctcctac caccatcaaa ccggttaacg ccgagattgc cgccttacag
     1501 cagcaaacgg cagatctgtt ttatgaaaat cgtctggtgc cgaaaaaagt cgatattcgc
     1561 cagcgcatct ggcagcccac tcaactggaa ggaaaacaat tatgagtctg aatatgttct
     1621 ggtttttacc gacccacggt gacgggcact atctgggaac ggaagaaggt tcacgcccgg
     1681 ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga tcgtctgggc tataccggtg
     1741 tgctgatccc aacggggcga tcgtgtgaag atgcgtggct ggtggcggcg tcgatgatcc
     1801 cggtgacgca gcggctgaag tttcttgtcg ccctgcgccc cagcgtaacc tcacctaccg
     1861 ttgccgctcg ccaggccgcc acgcttgacc gtctctcaaa tggtcgtgcg ttgtttaatc
     1921 tggtcacagg cagcgatcca caagagctgg caggcgacgg cgtgttcctt gatcatagcg
     1981 agcgctacga agcctcggcg gaatttaccc aggtctggcg gcgcttattg ctgggggaaa
     2041 ccgtcaattt caacggtaaa catattcatg tgcgcggagc aaaactgctc ttcccgccga
     2101 ttcaacagcc ttatcctccg ctttactttg gcggatcgtc agacgtcgcc caggagctgg
     2161 cggcagaaca agttgatctc tacctcacct ggggcgaacc gccagaacta gtaaaagaga
     2221 agatcgaaca agtgcgggcg aaagctgccg cgcatggacg caaaattcgt ttcggtattc
     2281 gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg gcaggccgcc gagcggttaa
     2341 tctcgcatct tgatgatgaa actatcgcca aagcacaggc cgcattcgcc cggacggatt
     2401 ccgtaggaca acagcgaatg gcggcgttac ataacggcaa gcgcgacaat ctggagatca
     2461 gccccaattt atgggcgggc gttggcttag tgcgcggcgg tgccggaacg gcgctggtgg
     2521 gcgatggtcc tacggtcgct gcgcgaatca acgaatacgc cgcgcttggc atcgacagct
     2581 ttgtgctttc gggctatccg catctggaag aagcgtatcg ggtcggcgag ttgctgttcc
     2641 cacatctgga tgtcgccatt ccggaaattc cccagccgca gccgctgaat ccgcaaggcg
     2701 aagcggtggc gaatgatttt atcccccgta aagtcgcgca aagttaagga agaaataatg
     2761 gcaacgccag tgaagaagtg gttattgcgc gttgccccct ggtttttacc ggtgggcatc
     2821 gtggcggtgt ggcaactggc ctcctcagtt ggctggctgt cgacgcgtat tttgccctca
     2881 ccggaagggg tggtgacggc gttctggacg ctctccgcca gcggcgaact gtggcagcat
     2941 ctggcgatca gttcctggcg ggcgctgatt ggcttttcaa ttggcggatc gctggggttg
     3001 attttgggac tgattagcgg gctatctcgc tggggagaac ggctgctgga tacctcaatt
     3061 cagatgttgc gcaacgtacc gcatctggcg ctgatcccgc tggtgatttt gtggtttggc
     3121 attgatgaat ccgcaaaaat ctttctggtg gcgctcggca cgctattccc catttatatc
     3181 aacacctggc atggcatccg taatatcgat cgcggtctgg tggaaatggc gcgtagctat
     3241 ggattatccg gcataccgct gtttatccat gtgatcctgc ctggtgccct gccctcaatt
     3301 atggtcggcg tgcgttttgc gttgggcctg atgtggctga cgctgattgt tgccgaaacc
     3361 atttctgcca attcaggcat tggttatctg gcgatgaatg cgcgggagtt tttgcaaacg
     3421 gacgtggtgg tagtcgctat tattctttac gccctgctcg gcaaacttgc tgacgtcagc
     3481 gcgcagctgt tagagcgcct ctggttgcgc tggaacccgg cttatcattt gaaggaggcc
     3541 acggtatgaa tactgctcgt ctgaaccagg gcacaccatt gttgctcaat gcagtaagca
     3601 aacattacgc ggaaaatatc gtcctgaacc aactggattt acatattccg gcaggtcagt
     3661 ttgtggcggt ggtgggccgc agcggtggtg gcaaaagtac cctgctgcgc ctgctggcag
     3721 gtctggaaac gccaaccgca ggcgatgtgt tagcgggcac cacaccgctg gctgagattc
     3781 aggaagacac gcgaatgatg tttcaggatg cgcgtctgct gccgtggaaa tcggtgattg
     3841 ataacgttgg gttaggcctt aaaggccagt ggcgcgatgc cgcgcgtcgg gcgttggctg
     3901 cagtaggact ggagaatcgc gccggggaat ggcctgccgc actttctggc gggcagaaac
     3961 agcgtgtggc gctggcaaga gcgttgattc atcgaccggg tttattgttg cttgatgaac
     4021 cgctcggggc gctggacgcc ttaacgcgac tcgagatgca ggatttgatt gtgtcacttt
     4081 ggcagcagca cggctttacc gtactgttgg tgacgcatga tgtgagcgaa gcggtagcaa
     4141 tggctgaccg ggtgctgtta attgaagagg gaaaaattgg tctggatttg acggtggata
     4201 tccctcgtcc gcgccggtta gggtcggtgc gattagcgga gctggaagcg gaagtattgc
     4261 aacgggtaat gcggcgtggg cattctgaac aactgattcg gcgtcatggt taatatgccg
     4321 gatgtggcgc acaaaaccag cattttcaac ggactagtag gcctgataag acacgtcaag
     4381 catcgcatca ggcagttggg cacggctgcc ggatgcggcg tgaacgcctt atccagccta
     4441 caaaaccagc aatttcaacg gagttgtagg cctgataaga cgcgtcaagc gtcgcatcag
     4501 gcattttggc tcaaaagtat caagccagtg ctttggtgat cttctcgtac agatcgccag
     4561 agagattttc cagccctttc aactgttcca gcgccgcgcg cattttctcc tgacgtttgg
     4621 catcataacg tttcaggcga atcagcggtt caatcagacg tgaagctacc tgcgggttac
     4681 ggctgttgag gtcggtaagc atttccacca ggaactggta accgctgcca tcttcggcat
     4741 ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa cgaacgaata cggttcgggt
     4801 tgctcatggt aaatgagcga tgctgcaaca gaccacgcac cgtctccagc acattcgccg
     4861 ccgggctggt ggcttgcagg ataaaccatt tatccatcac cagaccgtcc tgatgccact
     4921 tatcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag ctgtgcagca accgccgcag
     4981 aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg gtattgcttg ctcaccagca
     5041 cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag gcaggcatta cgcagagtgc
     5101 gttttgcaat atcttcatgc tcaacacggt actcgctctg gtaattcgcg ttgtaaatag
     5161 ccagcagctc atccgccagt tcagtcgcca gagtacgagt gagtgcttcg cgtacttcgg
     5221 caatagcaat cgggtcgatg atatcgaaca gttcagccat ttcattgaca gaaggcagcg
     5281 tcaggatttc tgccgccagc gccggatcaa tcttctcatc gagcagcacc gcgcggaaag
     5341 cgtcagccac atgtaccggc agagacagcg gctgcccttg ctgatgacgg gcgacgttca
     5401 gcttgatgta ggttgccagc aaactttgcg ccgcatccca acgggagaaa tcattacgcg
     5461 catgacgcat caggaaggtc agttgctgat cgctccactt atattccagt ttcactggcg
     5521 cagagaattc gcacagcagc gcaggcaccg gctggaagta gacattatca aaaacaaagg
     5581 tctgttccgc ctgggtgacg ttcagcacgg aattcaccgg atgaccgcct ttctgcaacg
     5641 ggatcacttt gccttcgttg tcatacaatt cgatggcaaa tggaatatgt agcggctgtt
     5701 tttctgcctg atccggcgtg gctggcgtgc gctggctgat ggtcagggtg tactgctcgg
     5761 tttccggatt gtagtcgtct ttgacggtca caatcggcgt gccggactgg ctgtaccaac
     5821 ggcggaaatg ggagagatcg acattcgacg catcttccat cgcctgcaca aagtcgtcgc
     5881 aggttgccgc gctgccatca tggcgctcga aatagagttg catccctttc tggaagtttt
     5941 cttcgcccag cagggtgtgg atcatgcgaa tcacttccgc gcccttctcg taaacggtca
     6001 gggtgtagaa gttgttcatc tcaatgacca tatccgggcg gatcgggtgc gccatcgggc
     6061 tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac attattgata cggtttaccg
     6121 cgcgggaacc aaggtcagag ctgaattcct gatcgcggaa gacggttaaa ccttctttca
     6181 ggctgagctg gaaccagtcg cggcaggtca ctcggttacc ggtccagtta tggaaatatt
     6241 catggccgat aacgcgttca atatcgagat aatctttgtc ggtggcggtg tcggtgcggg
     6301 ccaacacata tttggagtta aagatattca gccccttatt ctccattgcg cccatattga
     6361 agaaatccac cgcgacgatc atatagatgt cgaggtcata ctccaggcca aagcgttctt
     6421 catcccattt catggagttt ttcagcgagg tcatcgccca cggcgcgcga tcaaggttgc
     6481 cgcgatcgac gtacagctcc agtgctactt cgcgaccaga acgcgtggta aaggtatcgc
     6541 gcagtacatc aaagtcgcct gccaccagcg caaacaggta gcacggtttc gggaacgggt
     6601 cctgccactg tacccaatgg cgtccgtttt cgagttcgcc ttgcgcaacg cggttaccat
     6661 tggaaagcag gaagggatat ttgattttat cggcaataat tttggtggta aaacgcgcca
     6721 gcacgtccgg gcggtcgaga taatacgtaa tatggcggaa accttcggct tcacactggg
     6781 tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc cgtattcgcc gccgggctta
     6841 tttcattaat gatcttaagc gtaaaacgct ccggcaaatt actgatgacc agtgcgccct
     6901 cttcttcttt ccaggcggtc cacggctcat cattaatatg aacagaaacc agtttgaggt
     6961 cttcgccgtt gagacgaagc ggagcatctg atgcaccatg acggacagcc tggctgaccg
     7021 cggtaacgac cgtcttttgc gcgtcgaggt caaaggtcaa gtcaatatca gtaatctggt
     7081 aatccggcgc acgataatcg tgacggtatt tggcttgtgg ctgttgagtc ataaataacc
     7141 tttagcatct tttatagagt ctggtgttca gtctattcct gttgcgtaaa tcgcgctatg
     7201 cagaatcttc atcttttcag gtacaaacgc ctttattgct acatttttat aacatacacc
     7261 gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat cggggttcaa taaattgcga
     7321 aacaaggtat actccagcag ttcctgaaga tgtttattgt actaaacgct cctgtacgag
     7381 gacgctactg cgcacctatg acacaattcg cttctcctgt tctgcactcg ttgctggata
     7441 cagatgctta taagttgcat atgcagcaag ccgtgtttca tcactattac gatgtgcatg
     7501 tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg tatttatgcc gatgctattc
     7561 gtgaacagat tcaggcgatg cagcacctgc gcctgcagga tgatgaatat cagtggctct
     7621 ctgctctgcc tttctttaag gccgactatc ttaactggtt acgcgagttc cgctttaacc
     7681 cggaacaagt caccgtatcc aacgataatg gcaagctgga tattcgttta agcggcccgt
     7741 ggcgtgaagt catcctctgg gaagttcctt tgctggcggt tatcagtgaa atggtacatc
     7801 gctatcgctc accgcaggcc gacgttgcgc aagccctcga cacgctggaa agcaaattag
     7861 tcgacttctc ggcgttaacc gccggccttg atatgtcgcg cttccatctt atggattttg
     7921 gcacccgtcg ccgtttttct cgcgaagtac aagaaaccat cgttaagcgt ctgcaacagg
     7981 aatcctggtt cgtgggcacc agcaactacg atctggcgcg tcggctttcc ctcacgccga
     8041 tgggaacaca ggcacacgaa tggttccagg cacatcagca aatcagcccg gatctagcca
     8101 acagccagcg agctgcactt gctgcctggc tggaagaata tcccgaccaa cttggcattg
     8161 cattaaccga ctgcatcact atggatgctt tcctgcgtga tttcggtgtc gagttcgcca
     8221 gtcgctatca gggcctgcgt catgactctg gcgacccggt tgaatggggt gaaaaagcca
     8281 ttgcacatta tgaaaagctg ggaattgatc cacagagtaa aactctggtt ttctctgaca
     8341 atctggattt acgcaaagcg gttgagctat accgccactt ctcttcccgc gtgcaattaa
     8401 gttttggtat tgggactcgc ctgacctgcg atatccccca ggtaaaaccc ctgaatattg
     8461 tgattaagtt ggttgagtgt aacggtaaac cggtggcgaa actttctgac agccctggca
     8521 aaactatctg ccacgataaa gcgtttgttc gggcgctgcg caaagcgttc gaccttccgc
     8581 atattaaaaa agccagttaa tatcatcagg gagctaatcg gctccctttt tttaccttta
     8641 attccgaaat ctttcgctgc atttgcgaat tctgcttgtc tgattgcaga tgccaggtaa
     8701 cataggtatc cccccattta cgggtggaca agtgtttatt ttttccgact attaacagag
     8761 agaatattat gagcgttgtg cctgtagccg acgtactcca gggccgcgta gccgttgaca
     8821 gcgaagtcac cgtacgcgga tgggtacgta cccgccgaga ttcaaaagct ggcatctcct
     8881 tcctcgccgt ttatgacggt tcctgctttg atcctgtaca ggctgtcatc aataattctc
     8941 tgcccaatta caatgaagac gtcctgcgtc tgaccaccgg ctgctcggtc attgtgacgg
     9001 gtaaagtcgt ggcgtcgccg ggccaggggc aacaatttga aattcaggcc agcaaagttg
     9061 aagttgctgg ttgggttgaa gatccagaca cttacccgat ggcggcaaaa cgccacagca
     9121 ttgagtatct gcgtgaagtc gctcacctgc gtccgcgcac aaacctgatt ggtgccgtcg
     9181 cgcgcgttcg ccatacgctg gcgcaggcgc tgcatcgctt ctttaacgag cagggattct
     9241 tctgggtttc aacgccactg attaccgcat ctgataccga aggtgcaggc gaaatgttcc
     9301 gcgtttctac gctggatctg gaaaacctgc cgcgtaacga tcagggcaaa gtggatttcg
     9361 acaaagactt ctttggtaaa gagtctttcc tgaccgtatc tggccagttg aacggcgaaa
     9421 cctacgcttg cgcattatcc aaaatttata ccttcggccc gactttccgt gctgaaaact
     9481 ccaacaccag ccgtcacctg gcggaattct ggatgctgga gccggaagtg gcgtttgcta
     9541 acctgaacga tatcgcgggt ctggctgaag ccatgctgaa atatgtcttc aaagcggttc
     9601 tcgaagaacg cgcagacgac atgaaattct tcgctgaacg cgtagataaa gatgccgttt
     9661 cacgtctgga acgcttcatt gaggccgatt tcgcgcaggt ggattacacc gacgcagtga
     9721 ccattctcga aaactgcggc aggaagtttg aaaacccggt ttactggggc gtcgatctct
     9781 cttctgagca tgagcgttat ctggcggaag aacactttaa agcaccggta gtggttaaaa
     9841 actatccgaa agatattaaa gcgttctata tgcgcctcaa cgaagacggt aaaaccgttg
     9901 cggctatgga cgttctggct ccgggcatcg gtgagatcat tggtggctcc cagcgtgaag
     9961 aacgtctgga cgttctggac gagcgtatgc tggaaatggg cctgaataaa gaagattact
    10021 ggtggtatcg cgatctgcgt cgctacggta ctgttccgca ttcaggtttc ggtcttggtt
    10081 ttgaacgcct gattgcttac gtaactggcg tgcaaaacgt gcgtgatgtg attccgttcc
    10141 cacgtactcc gcgcaacgcc agcttctgat tttcttcctc aaagagccag cgcctgctgg
    10201 ctctttttta tcctttctgt caagttatct gtttgt
//
LOCUS       DAFAVF010000150        10207 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFAVF010000150 DAFAVF010000000
VERSION     DAFAVF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.150"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            249..2387
                     /locus_tag="LGK42_003632"
     CDS             249..2387
                     /locus_tag="LGK42_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK2659837.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTFQPDELISD
                     SFNILIRNQAHAEREREIEEMTQKIRAVFTDHTELNSLIDHLQELSNAFKSTSSGISR
                     SSTGMRGLSGGNKIHHIPAGLENYQPYIRSERRVEWIDWQTKGLEFSPLSDGCCPFCT
                     GDITGKEAQIRQVREEYDKSTIKNLTAIIRLVENLGNYLTESARERLLAITMLQNGPE
                     AEHIEYLVALKRQTDTLTEKLTALRGLNVFSLQEQQNVREVLTARLIDLQFFPDLQSE
                     LMQGITDRLNAALMDLINLAGPLQGKINRHRDSMIRLIAQHKTNINNFLTYAGYKYRV
                     DIAGEGEQRKLRLRHIDFDGYVSGGSQHLSYGERNAFAIVLFMYECLSKNPGLIILDD
                     PISSFDKNKKFAILEMLFRRASGECLKNRTVLMLTHDVEPVIDTLKSVRRLFSNQVTA
                     SCLRLSAGVIEELPVNDGDIMTFMQICKSITASADCEEIIKLIYLRRYFEIVDERGDA
                     YQLLSNLFHRRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALV
                     SSPDEIKNLYRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRF
                     DLIPEYVIMECDKLIALPPAANQSSVARIA"
     gene            complement(2549..2965)
                     /locus_tag="LGK42_003633"
     CDS             complement(2549..2965)
                     /locus_tag="LGK42_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBK2659838.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAIDATTKIKVALRLTGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2962..3192)
                     /locus_tag="LGK42_003634"
     CDS             complement(2962..3192)
                     /locus_tag="LGK42_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBK2659839.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            3564..4565
                     /locus_tag="LGK42_003635"
     CDS             3564..4565
                     /locus_tag="LGK42_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBK2659840.1"
                     /translation="MTEPLNLRTDVVRQHTVPRFLLKHFSKPVKGKRQRLHAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPQRFSLEPLLGIYEHDAAPVIAGLLEHKDIRRLTED
                     DRYKLAVFVAVQRARTFGELERISGMISVLTDKLAAMGATEEQAGETLGLSPGGDTRD
                     IFLRQLVQQVSHIKHLLSKDWYLLETRPEHPFYVSDNPVVLENRNDFGVYGNIGLAVP
                     GIQIYLPLSSTLMLAMYCPSIREQKVREKQHLLHLIARAPDLIPRHMRPFEMLEHVNR
                     HTDYLLMPLSAENVMHYNALQVEYAEQYVFCGENDFSLAERMLAADDRYRTGPRFTF"
     gene            complement(4749..5093)
                     /locus_tag="LGK42_003636"
     CDS             complement(4749..5093)
                     /locus_tag="LGK42_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBK2659841.1"
                     /translation="MKNLSDIVGMFAFPFQTEIAFVAAIYPTAKNLTVSTRGHIGQKQ
                     YWSAWLCVMLLMVYVGFAAAVVWAVVPWLKGFIVSKFAVSALWATPVKGALLWGVGKM
                     VAAIRRKITPSN"
     gene            complement(5590..5844)
                     /locus_tag="LGK42_003637"
     CDS             complement(5590..5844)
                     /locus_tag="LGK42_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659842.1"
                     /translation="MKGRQSRYVTGGESFAEIARRPGGTVVMLCLNPGLEEALREASK
                     SLKSAFSRSGRKCRLSAGTAEGPFAGRRQGTATHLFVAVL"
     gene            complement(5888..6814)
                     /locus_tag="LGK42_003638"
     CDS             complement(5888..6814)
                     /locus_tag="LGK42_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659843.1"
                     /translation="MSDKERNPVNAGGESVTYTLRNIPAELDRVISDLAISAGKPKAT
                     FLKEFLESSFHDVIDLFSLRSPLVATLDDVLASELGAQVMTQRYGSHFVTRWNREYQN
                     LLGISTEEELRRMLLNNTPYLRTRAVQVLKGWSAIPRGISLTFALFAELAGRDRATID
                     QAWTSIFYSQLPEARHRFYRDIDTIRALKKQHAVCGYSQTRDDITVRIYRPDDYGHGA
                     WRVLLVLDESVITQTWNIPFPELDGRRFTTDPGYDALISTAPDSWDKAFCFVDGICEL
                     HLYSNGVAEEQNPTPLPAVAEALINAVVHELL"
     gene            6970..7242
                     /locus_tag="LGK42_003639"
                     /pseudo
     CDS             6970..7242
                     /locus_tag="LGK42_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493379.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7312..7920
                     /locus_tag="LGK42_003640"
     CDS             7312..7920
                     /locus_tag="LGK42_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659844.1"
                     /translation="MKKGKTLEPGLLASDSDWHNNACLNYMPDHGTAYTEGYRRAADI
                     LINHIDESGRDQDFLVYPVLFLYRHHLELLIKQIIGLALALAEDPDKHQYKKDDHNLN
                     NLWPLAQKLILEVDDSYRPSDFKIVKEVVKALHQADERATDFRYAKRNDGTRSLEGIH
                     YVNTRRFGKKMGEASDLLDGVDNGLRYLLDCKAEWNQILDSF"
     gene            complement(8281..9468)
                     /locus_tag="LGK42_003641"
     CDS             complement(8281..9468)
                     /locus_tag="LGK42_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBK2659845.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEDSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQFWSLVFHNRCLLAEMSRIAADVILEICRQADVEPGGFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLNTHYRRGWNVNVSQVMDNATHVAVYFGSYLKKPQVPMSRLEHYAGQDEVGLR
                     YNSHRTRREEYLVMSGDEFMERFSWHMADKGFRMVRYYGFLSPVKRRLLEEVVYTITE
                     TVRKTARQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAELVMMHEPLARMR
                     WCG"
BASE COUNT         2560 a         2665 c         2542 g         2440 t
ORIGIN      
        1 atgaaaatca tatctgatat gattttatgt gaattgctta attatcatat cgtttgcccg
       61 tttcccgtcg tgcagatccg gtttccggct aagtcggatc gacctaatct ttgtttctga
      121 tatgaacacc tcggagaggt gtaagcaatg gtataaacga aatgtattat gtatctgcat
      181 cacagcatgc gggcacattt tgcggcagga tatgaatccc cccggaataa ctggcaaagg
      241 acagatcaat ggacattgaa atacgccact gcaataacat cgtacgagca catatcaccc
      301 tcactgccga taaactgaac attaagtttg cgccaaatgg tactggaaaa agtacgctgt
      361 cacgggccat aagctgtgcg gcgcgggacg acattcaggg actacaggca ctgatgccct
      421 tccggctgcg cggagaaaac cccgatagca ccgggcccat tgtcatcggt gctgacggga
      481 ttggggacgt gatgtgcttc aacgaggagt acgtcagtca gtttacgttc caaccggacg
      541 agctcatcag tgacagcttt aatattctta tccgcaatca agcccatgcg gaaagagagc
      601 gtgaaataga agaaatgacg caaaagatcc gggctgtttt cacggatcac actgagctga
      661 actctctgat agaccatcta caggaactca gtaatgcttt caaatcaacc agttccggga
      721 tttcacgttc ttcaaccggt atgagaggtc tgtcaggcgg aaataaaata caccacattc
      781 cggctggtct tgagaactat caaccctaca tccgcagtga acgccgcgtg gaatggattg
      841 actggcagac aaagggactc gagttttcgc ccctttcgga tggctgctgt ccgttctgta
      901 ccggtgatat cacgggaaag gaagcacaaa ttcgccaagt cagagaggaa tacgataaat
      961 ccaccattaa aaacctgacc gctatcatca gactggtgga aaacctcggt aattacctga
     1021 cggagagcgc cagagaacgc cttctggcca tcactatgct tcagaacggt ccggaagccg
     1081 aacatatcga gtacctagtg gcgttgaaac gccagaccga tacgctgaca gagaaactca
     1141 ctgcgctgag aggcctgaat gttttcagcc tgcaggaaca gcagaacgtt cgggaggtgc
     1201 tcactgccag gttgattgac ctgcagttct tccctgatct gcaatccgaa ctcatgcagg
     1261 ggatcaccga cagactgaac gcggccctta tggacctgat aaaccttgcc ggaccgctgc
     1321 agggaaaaat taaccggcac cgggacagca tgatccggct gatcgcacag cacaaaacaa
     1381 acatcaataa tttcctcact tatgcaggct ataaatacag ggtggatata gccggcgagg
     1441 gagagcagag aaaactcagg ctacgacaca tagattttga cgggtacgtc agcggcggta
     1501 gccagcatct cagctatgga gaacggaacg cctttgcgat tgtgttattc atgtatgaat
     1561 gcctgtcaaa aaacccggga ctgattatcc ttgatgatcc catatcctct tttgataaaa
     1621 acaagaagtt tgccattctt gaaatgctgt tcagacgtgc aagtggtgaa tgcctgaaga
     1681 accggactgt acttatgctg acgcatgacg tggagcctgt gattgacacg ctgaagtcag
     1741 tcagaaggtt gttcagtaat caggtgaccg cctcctgcct gcgcctgtct gctggagtca
     1801 tagaagaatt acctgttaac gacggcgata tcatgacatt catgcagatc tgcaaatcca
     1861 tcactgcgtc agcagactgt gaggagatca ttaagctcat ctatcttcgt cgctactttg
     1921 aaatcgttga cgaacgtggt gatgcttatc agcttctatc caatctattc catcgacgcg
     1981 tcgccccgct ggattatcgc gagcctgccg ctgcgggctc gggctacccc aaaatggctc
     2041 ctgagaaaat tcagcaggcc ttgcgggata tccgggagta tgtggacagt tttgattacc
     2101 cgcgacttca ggctctcgtc agcagcccag atgaaataaa aaacctgtac cgtcgctgtc
     2161 gtaacggcta tgaaaaactg caggtattcc gtttactgga actggatcag gaccatccgg
     2221 taatacgaaa attcgttaac gaaacgtacc acattgaaaa tgagttcatc tgtcaactag
     2281 atccgtccag attcgatctc atcccggagt acgtgattat ggaatgtgac aagctcatcg
     2341 ccctgccacc ggcagcaaac cagagctcgg ttgcccgtat cgcttgagcc actgtttttg
     2401 ccgccggaag ataggcttta gctccctgat agtctgatct tcgaagatac cctacagttt
     2461 aggaccgctt actgtagata tctgttaaaa cagcccgtat acgggctgtt tcttggtgcc
     2521 cttatctcct ggctttgaaa agagtgtctt acttcaccca gtcttccagc accaggtcag
     2581 gcacccgctc aaattctctt gtattattcg ttaccagtat cgccccggcg gcgatggcgt
     2641 gcccggcaat cgccgtgtcg ttcgggccga tcggcgtccc ggtcagccgc agtgccactt
     2701 taatcttcgt ggtggcgtct attgcggcgc gatcccaggg caggatggca tcgaggcggg
     2761 cgcagaattc atcgaccagt tggacgtgac gtggtgaggc ctttgggccg gtagccccga
     2821 agcgcatttc agagtaggtg atggccgaga ccactatacg gtggccgcgt agcaccgcct
     2881 gctccagacg cttcaggacg gcttctggct gttcacgcat gatgaacgag cagatacagg
     2941 tatcaagcat ataagtcttg ttcacaggtt aaatcgccct tcgtcgctga caacatcctc
     3001 gcgctccgcc ataaaatctg gatcggcttt ttcgtactct aggaatgatc cccaggttgg
     3061 ccggacgggg cgcaggatga tgctgtcccc ttcccggacg atctcgagct cgctcacccc
     3121 ctcaaaatcc agatcacgag gcagacggat ggcgcggttg ttgccatttt taaatatcga
     3181 tacggttctc atgttctttc tctcccgagc atatggatat gtgaaacata tccggaatat
     3241 tcaaagcacc gggtgtgtgc atatgtttag tataggcaga tgtgtggcat atgtatagcg
     3301 gtagcatatg tagatgctag gcatatgttt agcatatctc tatttctttc ttttttatcg
     3361 gagatccgaa aaaaggttta accggaagca agagattccc cttaagactc acgatataat
     3421 tctttcggag atgtcaagcg tccccggcaa agactcaatg attacggcgt tacattgatg
     3481 tgttaatgtt ctgctcacca cataatagat tcgctggtcc acggtgaatc tacagtacca
     3541 gtccatcaag gatgataatt tccatgaccg aaccattaaa cctgcggacc gacgttgtca
     3601 ggcagcacac agtcccccgt tttttactga agcacttcag caagccggta aagggaaaac
     3661 gccagcgttt acacgccttt gataaggctg ccgggcgtgc ttatgccacc acgccggatg
     3721 acgccaccgt ccggaatacg ttctacaacc tcgataatca ccctcaacga ttcagtcttg
     3781 aaccgttgtt ggggatttac gaacatgacg cggcaccggt cattgccggc ctactggagc
     3841 ataaggacat tcgccggctg actgaagatg accgctacaa gctggccgtg tttgttgccg
     3901 tgcagcgcgc acgtaccttc ggtgagctgg aacgtatctc aggcatgatt tcggtgctga
     3961 ctgataagct ggcggcaatg ggcgcgactg aggagcaggc aggagaaact ctgggtttat
     4021 cgcccggggg cgataccaga gacatttttc tgcggcagct tgtccagcag gtgtcgcata
     4081 ttaaacacct gctaagtaag gactggtacc tcctggaaac caggccagaa cacccgttct
     4141 atgtgtcaga caatccggtg gtactggaga accgtaatga cttcggtgtg tacggtaata
     4201 tcggcctagc cgtgcccggc atacaaatct atctgcccct gtcatcgacg ctgatgctgg
     4261 ccatgtattg cccttccatc cgtgaacaga aagttcgtga aaagcagcat ctccttcatc
     4321 ttattgccag agcgccggat ctaatcccgc gtcacatgcg cccgtttgaa atgctcgagc
     4381 atgtcaacag gcatacggac tatcttctta tgccactttc tgcagaaaat gtgatgcatt
     4441 ataacgcgct gcaggtggag tatgcggaac agtacgtgtt ctgcggtgaa aacgattttt
     4501 ctctggctga aagaatgctg gctgcagatg atcgttaccg gaccgggccg cggtttacat
     4561 tttagggcaa cttccggtca gcgcagtgcc gcaaaatgcc gatgtccgtt cctgcgggcc
     4621 atcccggcaa cgatgatttt gccgtgaagc acccacgcga aagcggcagc ggtcacaggg
     4681 accaccgccg ggaggtgagg atgaatatct gttgacattc accctggatt caggtaggtt
     4741 tgcttctctt agttgcttgg tgtaattttt cggcggatgg ctgcaaccat cttaccgaca
     4801 ccccagagaa gcgcaccttt tactggcgtg gcccacagtg ccgaaacggc aaatttcgac
     4861 acaataaagc ccttaagcca gggcactaca gcccagacta ccgcagcagc aaatcccacg
     4921 taaaccataa gaagcatgac gcagagccat gcgctccagt attgcttctg accgatgtgc
     4981 ccccgggtac tgacggtaag gtttttagcg gtggggtaaa tcgctgctac aaaagcgatt
     5041 tcagtctgga acgggaatgc gaacattccc acgatgtcgg acaaattttt catagttccc
     5101 tcctgggtaa ttagttgaaa aatcaaatgt atttagcttg gtcatctcaa taacctcatg
     5161 tttaagtcaa aaggcatcct gcgcctcacg tgacgtgaac gctgctgata ccgtacggaa
     5221 tatgatgctt cctggtcctg ctatgatgtg ttcatttgct tcctcctggt tgggtgaact
     5281 gatttaatct cgtcctgcct gattcctgaa aaatccaaaa aaatccacac cggaatgacg
     5341 ggtgtcagtt ctggagagaa atgtttcttt tcgggggaat agcacgggcg acaggatgct
     5401 gtggaattat cttatcggca atacagcact ttgcaaagct tattaacaca aattttctca
     5461 ctataacaaa atattgccag tccgctttta acaaaaagtg ctgtttaccc cgtatcaggt
     5521 gagcttattt tgtcccgggg aggggcagcc aggcaaatgc gtttttcagg tttttgcccc
     5581 ctgcccccct cagagcaccg ccacaaacag atgcgtggcg gttccctgcc tgcgaccggc
     5641 aaacggccct tccgccgttc cggctgacag ccggcattta cggccgctcc ggctgaaggc
     5701 cgacttgagg gatttggatg cctcccgcag cgcctcctca agcccggggt tcaggcagag
     5761 catcaccacc gtccctcccg ggcgacgggc aatctccgcg aaactctccc cgccggtaac
     5821 atagcggctc tgccgacctt tcataatgcc tcctgttgat gcagttgtgc ccgtacaccc
     5881 gggtccgtta aagtagttcg tgcaccaccg cgttgatcag cgcctcagcg acggccggca
     5941 gtggggtggg attctgctct tctgccacgc cattgctgta aaggtgcagc tcacaaatcc
     6001 cgtccacaaa acagaaggcc ttatcccagc tgtcgggtgc ggtgctgatc agggcgtcat
     6061 agcccggatc agtggtaaag cgacgtccgt caagctcagg gaacgggatg ttccaggtct
     6121 gggtaatgac actttcatcc agaaccagca gcacccgcca ggcaccatgg ccgtagtcat
     6181 ccgggcggta aatgcgcacg gtgatgtcat ccctggtctg gctgtatccg cagacggcat
     6241 gctgtttctt cagggcacgg atggtatcga tatcccggta aaaacggtgt ctggcctcgg
     6301 gcagctgtga gtaaaaaatg ctggtccagg cctggtcgat ggtcgccctg tcccggccag
     6361 caagctccgc gaacagggcg aaagtcaggg agatcccccg gggaatggca gaccagcctt
     6421 tcagcacctg gacggcacgt gtccgcagat agggggtgtt gttcagcaac atacggcgca
     6481 gttcctcttc cgtcgagatc cccaggaggt tctggtactc ccggttccag cgggtgacaa
     6541 aatggctccc gtaacgctgg gtcatgactt gcgcgcccag ctcactggcc agcacgtcat
     6601 cgagtgtggc caccagggga ctgcggaggg aaaacagatc aatcacgtcg tggaagctgg
     6661 actccagaaa ctccttcaga aatgtcgcct tgggcttacc ggcagagatc gccagatcgg
     6721 agatcacccg gtcaagttca gccggaatat tccgcagcgt gtacgttacg ctctctcccc
     6781 ctgcatttac tggattgcgt tccttatcgc tcatcgcgtt caccttttct tctttgggtg
     6841 catatttata ttgagtagac tctatcccgg aaaggtgatg cattaaatac tgcgaagcat
     6901 acttttttta aaaacgtata cgattgtgta ttttttgttc atgtttgttt ttgcagatcc
     6961 gaaagccctg aaaataatgt ttatgctgca acagaattag gtcttggcat tctgctccgt
     7021 aaatacgagc agtttcgccg ggagctggat gcggccggtg agctgggaaa ctgggaatat
     7081 gatctcgata cctacaatca ctgtattgcg gtgctacaac gttacttcac cggtaatcca
     7141 tccggactga cagaacgtga tgcccggatt tacagtcatt acctgcagac tgagcacaaa
     7201 gggtttgtga agctggctga agaactggct gctgactgct gaatacatac agtaggcggg
     7261 atttccggat aaggtaccga accggggctg ttaaaagatt ctggagctga aatgaaaaag
     7321 ggaaaaacgt tagaaccggg gctgctggcc agcgacagcg actggcacaa taatgcctgc
     7381 ctgaattaca tgccggatca tggtacagct tacactgaag gttacaggcg agctgctgat
     7441 attctgatta accacattga tgaatccggg cgggaccagg attttctggt ttatccggtg
     7501 ttgtttctct accgacatca cttggagctc cttatcaaac aaattatcgg actggccctt
     7561 gcactggcag aagacccgga taaacaccag tacaaaaaag atgaccataa cctgaataat
     7621 ctatggccgc tggcacaaaa gctgatcctg gaagttgatg acagctaccg gccttccgat
     7681 tttaaaatcg tcaaagaggt ggttaaagct cttcaccaag cggatgaacg ggcgacagat
     7741 ttccgatatg ccaagagaaa tgacggcacc cggagccttg aaggaattca ttacgtcaac
     7801 acccgccgct ttgggaaaaa aatgggagag gcttccgatt tacttgacgg ggtcgacaac
     7861 ggcctccggt acctgctgga ctgtaaggcc gaatggaatc aaattctgga cagcttctga
     7921 cagccagaaa cggcgcgaaa aatccatcag acttacctga acaactaatg atactgagat
     7981 accgatcatg actggcctca taccttcagc tacttctcag ggctcaatgg ccgccctgcc
     8041 agtgaccatc gactatccca tagcactggc cttacggcag atggcgctcg ttcatcgaca
     8101 ggaatttaaa ccctaaaaag attaaaaaaa caccaaaaaa cggatgttta ttcaacactg
     8161 cctgcgtgcc atgagtgcac cgccgtcaat taaatggact gcattgattg ataccgggta
     8221 tcagtgatgg ttttttgctc catttcccgg acaatggatg caacttcccc gatgcggctc
     8281 tcagccgcac caccgcatcc tggccagtgg ctcatgcatc ataaccagct ctgccagacg
     8341 gtatccccgc ttcagccccg taaaacgcat ctgacttccg cacagcacgc atttcagcgg
     8401 gtcaaccttc agtaaccgct gatacatccc cctccatgtt atttgccttg ctgtttttct
     8461 cacggtctcc gttatggtgt ataccacctc ttccagtaag cgccgcttca ccggactcag
     8521 gaacccgtag tacctcacca tacggaaccc cttatctgcc atatgccacg agaacctttc
     8581 cataaactca tcaccgctca tcacaaggta ctcttcccgc cgtgtgcggt ggctgttata
     8641 acgcagaccg acttcatcct gaccggcata atgctccaga cgactcatcg gcacctgtgg
     8701 ctttttcagg taagagccga agtacaccgc cacatgtgtg gcgttatcca tcacctggga
     8761 tacgttgaca ttccagccac ggcggtaatg cgtgttcagg aagcgattcc agtcccgttt
     8821 actgcttcct tctgctgcca gcgcatccgg tatcaccagg tccggatatt tccgtgacag
     8881 caaccgcgtt atccggtaac gccacatgct catcacctta cgggcgtaaa aatgaaggtt
     8941 tttccaggta tgacctgacg tcacaccacc ggcagtggtc gacagatgaa tatgtggatg
     9001 ccattgctgg tcacggcccc atgtgtggat taccgtgaat ccccccggtt ccacatccgc
     9061 ctggcggcag atttccagta tcacatccgc tgcaatacgg ctcatctctg ccagtaagca
     9121 ccggttgtgg aacaccaggg accagaactg gcagggaagt gtgaacacaa tatgctgcca
     9181 cgggcaatcg gggaccagac tcagcagata ctgtatccac tgtgcgccag ccttcactcc
     9241 gcagtgcggg caggagcggc ttttacaccg gaagcagacc ttttttgtgt ggcaacagtc
     9301 cggtgatgaa cagcaccact gtgtgtaacc catcagcgtg gtcccgcagg ccatgatttt
     9361 tgtcactgac tcaatcacca ccggacgcac tgaatcttcc ggctgcttct ccagccagtt
     9421 aagccagcgg tttccctgct ggaaaatatc ggcaaaacgg ggaagcatga attacgcagg
     9481 gggaggatgt gaggtgccgc actgcggaca gataaaggga tgtatcgtgc acacggacag
     9541 aaaatttttc caggacatgg ggagcatggt gacattaccg tcgggctcac caactatcca
     9601 tacagttgtt tcataattgt cggtggcatc ttcttcaaac aggtatggaa tatctttctc
     9661 catacagtaa tcggcagcgc tggcgaagtc gggaaaaaac agcccggctt cattatctgt
     9721 tcctccggcg caggggacca cccgccatcg caacaggggg ggctgagcgg gaagattgtc
     9781 tgctggattt ttgtttgcga ggtttagctg aacgggctat ataaccacct gaaagacaat
     9841 gatattgtct gtttttataa cggtaattac aggccatgac aagccgcagc cgccaggctg
     9901 cctactcgat tatgcttttg atatgtcagg taattacttc tctaatatac gtttttatat
     9961 cattggccat caattcccat atacttctaa agccatcaca acaaaataaa taaagcaaaa
    10021 acatacacaa atataagaga gtttaactat caaataaatg ataaccaatc aaactgcagt
    10081 gccagcgata tgccgtgata atgactcgac cttaaacaaa atctaaacaa cagagcaata
    10141 tacaattgac tcatcaagtt agatataaaa aatacatgtt aaatcattaa aacgattgaa
    10201 tggagat
//
LOCUS       DAFAVF010000151        10162 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFAVF010000151 DAFAVF010000000
VERSION     DAFAVF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.151"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(18..93)
                     /locus_tag="LGK42_003642"
     tRNA            complement(18..93)
                     /locus_tag="LGK42_003642"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Lys,seq:ttt)
     gene            complement(127..202)
                     /locus_tag="LGK42_003643"
     tRNA            complement(127..202)
                     /locus_tag="LGK42_003643"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Lys,seq:ttt)
     gene            complement(349..424)
                     /locus_tag="LGK42_003644"
     tRNA            complement(349..424)
                     /locus_tag="LGK42_003644"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:Lys,seq:ttt)
     gene            complement(428..503)
                     /locus_tag="LGK42_003645"
     tRNA            complement(428..503)
                     /locus_tag="LGK42_003645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Val,seq:tac)
     gene            complement(654..729)
                     /locus_tag="LGK42_003646"
     tRNA            complement(654..729)
                     /locus_tag="LGK42_003646"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Lys,seq:ttt)
     gene            complement(732..807)
                     /locus_tag="LGK42_003647"
     tRNA            complement(732..807)
                     /locus_tag="LGK42_003647"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Val,seq:tac)
     gene            complement(943..1018)
                     /locus_tag="LGK42_003648"
     tRNA            complement(943..1018)
                     /locus_tag="LGK42_003648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(983..985),aa:Lys,seq:ttt)
     gene            complement(1183..1974)
                     /gene="cpoB"
                     /locus_tag="LGK42_003649"
     CDS             complement(1183..1974)
                     /gene="cpoB"
                     /locus_tag="LGK42_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBK2659846.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1984..2505)
                     /gene="pal"
                     /locus_tag="LGK42_003650"
     CDS             complement(1984..2505)
                     /gene="pal"
                     /locus_tag="LGK42_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBK2659847.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2540..3832)
                     /gene="tolB"
                     /locus_tag="LGK42_003651"
     CDS             complement(2540..3832)
                     /gene="tolB"
                     /locus_tag="LGK42_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBK2659848.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3965..5230)
                     /gene="tolA"
                     /locus_tag="LGK42_003652"
     CDS             complement(3965..5230)
                     /gene="tolA"
                     /locus_tag="LGK42_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBK2659849.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5295..5723)
                     /gene="tolR"
                     /locus_tag="LGK42_003653"
     CDS             complement(5295..5723)
                     /gene="tolR"
                     /locus_tag="LGK42_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBK2659850.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5727..6419)
                     /gene="tolQ"
                     /locus_tag="LGK42_003654"
     CDS             complement(5727..6419)
                     /gene="tolQ"
                     /locus_tag="LGK42_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBK2659851.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6416..6820)
                     /gene="ybgC"
                     /locus_tag="LGK42_003655"
     CDS             complement(6416..6820)
                     /gene="ybgC"
                     /locus_tag="LGK42_003655"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBK2659852.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6970..7263)
                     /gene="ybgE"
                     /locus_tag="LGK42_003656"
     CDS             complement(6970..7263)
                     /gene="ybgE"
                     /locus_tag="LGK42_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBK2659853.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7263..7376)
                     /gene="cydX"
                     /locus_tag="LGK42_003657"
     CDS             complement(7263..7376)
                     /gene="cydX"
                     /locus_tag="LGK42_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBK2659854.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7391..8530)
                     /gene="cydB"
                     /locus_tag="LGK42_003658"
     CDS             complement(7391..8530)
                     /gene="cydB"
                     /locus_tag="LGK42_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBK2659855.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8546..10114)
                     /gene="cydA"
                     /locus_tag="LGK42_003659"
     CDS             complement(8546..10114)
                     /gene="cydA"
                     /locus_tag="LGK42_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBK2659856.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2455 a         2776 c         2454 g         2477 t
ORIGIN      
        1 agggcgcttt tttacattgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
       61 agtcaactgc tctaccaact gagctaacga ccccttgcgg gattatgctg ctttaatcat
      121 tcaggatggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
      181 ctaccaactg agctaacgac ccatacgggt gttacctgaa agatttctac tcggggccaa
      241 tctttaagat tggtgtgtcg tgcaggatga ctcgcttcgc ttgccctacg ggccgttgct
      301 ggcgcaacgt tatccttcac gcttaacatc tgagtttgat gttaaaattg gtgggtcgtg
      361 caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg
      421 acccgagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta agggaggtgc
      481 tctcccagct gagctaatca cccgatactg cgctggatac tacaaactac ttccaccgaa
      541 cccaccgtaa ttagtggtgg gtcgtgcagg atgactcgct tcgctcgccc tacgggccgt
      601 tgctgacgca acgttatcct acacgcttaa catcttgtct tcgatgctaa aactggtggg
      661 tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc
      721 taacgacccg gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag
      781 gtgctctccc agctgagcta atcacccaat actacgctgg atactacaac taactatgtt
      841 agtggtgggt cgtgcaggat gactcgactt cgtctcgccc ttcgggccgt tgctgatgca
      901 acgttatcct tcacgtttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat
      961 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac
     1021 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
     1081 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
     1141 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
     1201 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
     1261 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
     1321 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
     1381 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
     1441 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
     1501 gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct
     1561 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
     1621 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
     1681 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
     1741 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
     1801 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
     1861 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
     1921 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
     1981 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
     2041 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
     2101 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
     2161 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     2221 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     2281 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     2341 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     2401 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     2461 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     2521 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     2581 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     2641 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     2701 catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     2761 tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     2821 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     2881 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat
     2941 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     3001 cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca
     3061 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     3121 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     3181 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg
     3241 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     3301 actgaccgcc gttggtctga acaacgtagg caatacgagt acggaacgca cctttaatgc
     3361 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct
     3421 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     3481 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
     3541 cgtcaatgcc cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     3601 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     3661 cgatgccgcc aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac
     3721 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     3781 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     3841 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     3901 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc
     3961 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     4021 gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
     4081 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     4141 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     4201 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     4261 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     4321 cagagcttag ctcaccgaaa atatcatctg cctctgcggc cgcttttgct gcagcggctt
     4381 tttcagctgc agcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     4441 ctttcttatc agctgctgcc ttttcagcag ccgctttttc tgctgctttt ttgtcggctg
     4501 cagctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     4561 cagcttctgc tttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg
     4621 cagctgcttc tgccgcttcc gctttcttct tcagtgccgc agcggctgcc tcggcttttt
     4681 tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc ttttttcttt gcgtctgcag
     4741 ccgctttctt cgctgcttct tccgcagctt tagcatctgc ttcggccttc gctttagcat
     4801 ctgccgccgc tttcgctgcc gcctcttcag cttgcttctg ctttaactcg gcctgttttg
     4861 cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc tcaagttgct
     4921 tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgttcct
     4981 tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc ctggctttgc atgcgtttgt
     5041 actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc
     5101 ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgcc gcaaataaga
     5161 tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg
     5221 cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag
     5281 ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta
     5341 tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc
     5401 agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct
     5461 ggtggtaaac gctccaggcg atctttctca accaccacgg tgtactgacc aataccagac
     5521 acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct
     5581 ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac
     5641 agcaccagca gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga
     5701 cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc
     5761 tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg
     5821 tttacgcgct ggttgaggcg gttataagcc attaccgccg gaattgcggc gaacagacca
     5881 attgcagtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt
     5941 tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca
     6001 atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca
     6061 agttcacggt tcatggagat acgcatagca cgcgacgccc cttccactac ggcttccggc
     6121 gcatggctat tggcacgatg caggcgcaca aactctttga acccgctgta aaagatttgt
     6181 tccgaaccag tcagattatc ccgtttcccc tggctctctt gatagaggcg agagagttcg
     6241 attccagacc agaatttatc ttcaaacgct tcggcttcgc gcgccgctgc gttaagaata
     6301 cgggtccgct ggataataat ggcccaagat gcgattgaaa aaccaatcaa aatcaacatg
     6361 ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact
     6421 gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa
     6481 cgcaaacaac cagaacctct gcttcattca gcaaagtatt ctcggcgttg acaatacgtt
     6541 gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat
     6601 cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc
     6661 gttcagccat cagcgcctgc tgactgaagt gatgatgacg cagcatctct gtgcgtgctc
     6721 tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat
     6781 agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa
     6841 caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag
     6901 ggcaagttaa gggagcgtaa aaatttatgg gcctggagcg gcccataatt ttttaaagag
     6961 aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa
     7021 tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga aagcccacgc cgtgaatcac
     7081 accagcacag acggcccaca tcagcaataa accatgccag atttccagtt cactggtctt
     7141 cgcggcaaaa cgagatgggt cccaaaacat acatcctgct aacagaagcg ccatcacgaa
     7201 ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaagtcgcga taatcttact
     7261 catcagatgt cttcttgacc ggctttgcct gattcgacgt gttcaagcgc cagcgcggtg
     7321 attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt
     7381 agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac
     7441 cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg
     7501 caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac
     7561 ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata ccggctgtca
     7621 ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac
     7681 gtgcagtcag gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa
     7741 ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca
     7801 gtgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt
     7861 acatcaccca tacgccagcc agtgcgaaac agaccagtgt caccagcgca gccacctgag
     7921 ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg catttgcaga taggttgcgc
     7981 cctgagtaat gatcatccct acgctcacca cgcctgccag caggccgaac gggttaagca
     8041 actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg aacggtacgc
     8101 cctgcaacag gttaccgaac gctacaccaa ttaccagcgg cggaacgaag ctaccaatga
     8161 agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa
     8221 aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg
     8281 agaacgcagc ggcatagacc atcggccagg cagcaaagag tgcgccgccc gcggtgatca
     8341 gccaaacctg gttaccgtcc cagtgtggtg caatggagtt aatcataatt cgacgctcgg
     8401 tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga
     8461 ctgcaaaacc aatcagcaga acgccaacca gcagccacca gataaaacgc aatacttcat
     8521 aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct
     8581 caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaat ttgaacatta
     8641 agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc attgagaaga
     8701 tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag
     8761 cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca atccacggca
     8821 gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt ctcgccaatg cggttgcgga
     8881 tgacactcca gaaagagagc gcgatgattg ccagaagcag gaagccacac gccaccatga
     8941 tacggaacgc aaagtacagc ggcgctacac gcgggatgga gtctttggtt gcctgttgaa
     9001 tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac
     9061 cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc ctggtcggta gaaccagaac
     9121 gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca
     9181 ccatcagctc tttcaggccg ataaccgggg tatccacgga acgcgttgca atgatgccca
     9241 gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga
     9301 acagagtaaa ggcagcaggc gcaggttgcg tttcccactc ggcttcaata gcagccagtt
     9361 tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca ataacagaca
     9421 gaacagcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt
     9481 cacgaccttt cagcatatac catgcgctga taccgaggat gaacatcgcg ccagtcacat
     9541 aaccagacgc tacagtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg
     9601 agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt
     9661 gcatccagcc gttcgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca
     9721 gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac
     9781 ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga
     9841 agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg
     9901 tcagaccggt agccacaccc agagcgaagt tgataccaaa caacttgccc cagaacttgg
     9961 tcatatcttt ataaatctgt ttgccggaga ggacgtagac cgtttccata atggccagca
    10021 ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa
    10081 actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag
    10141 actccgagag tgaaccccgt ta
//
LOCUS       DAFAVF010000152        10068 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFAVF010000152 DAFAVF010000000
VERSION     DAFAVF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.152"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(24..1568)
                     /gene="ilvA"
                     /locus_tag="LGK42_003660"
     CDS             complement(24..1568)
                     /gene="ilvA"
                     /locus_tag="LGK42_003660"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBK2659857.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(1571..3421)
                     /gene="ilvD"
                     /locus_tag="LGK42_003661"
     CDS             complement(1571..3421)
                     /gene="ilvD"
                     /locus_tag="LGK42_003661"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBK2659858.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(3486..4415)
                     /gene="ilvE"
                     /locus_tag="LGK42_003662"
     CDS             complement(3486..4415)
                     /gene="ilvE"
                     /locus_tag="LGK42_003662"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBK2659859.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(4435..4698)
                     /gene="ilvM"
                     /locus_tag="LGK42_003663"
     CDS             complement(4435..4698)
                     /gene="ilvM"
                     /locus_tag="LGK42_003663"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBK2659860.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(4695..6341)
                     /locus_tag="LGK42_003664"
     CDS             complement(4695..6341)
                     /locus_tag="LGK42_003664"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBK2659861.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQVRQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(6344..6394)
                     /locus_tag="LGK42_003665"
     CDS             complement(6344..6394)
                     /locus_tag="LGK42_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659862.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(6481..6579)
                     /gene="ilvL"
                     /locus_tag="LGK42_003666"
     CDS             complement(6481..6579)
                     /gene="ilvL"
                     /locus_tag="LGK42_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBK2659863.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            6932..8452
                     /locus_tag="LGK42_003667"
     CDS             6932..8452
                     /locus_tag="LGK42_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBK2659864.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(8477..8815)
                     /gene="maoP"
                     /locus_tag="LGK42_003668"
     CDS             complement(8477..8815)
                     /gene="maoP"
                     /locus_tag="LGK42_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBK2659865.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            8934..9773
                     /gene="hdfR"
                     /locus_tag="LGK42_003669"
     CDS             8934..9773
                     /gene="hdfR"
                     /locus_tag="LGK42_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBK2659866.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(9869..9944)
                     /locus_tag="LGK42_003670"
     tRNA            complement(9869..9944)
                     /locus_tag="LGK42_003670"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9909..9911),aa:Trp,seq:cca)
     gene            complement(9953..10029)
                     /locus_tag="LGK42_003671"
     tRNA            complement(9953..10029)
                     /locus_tag="LGK42_003671"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9993..9995),aa:Asp,seq:gtc)
BASE COUNT         2364 a         2724 c         2604 g         2376 t
ORIGIN      
        1 gaagcgctat caggcatttt tccctaaccc gccaaaaaga acctgaacgc cggattattg
       61 gtttcgtcgt ggcaatcgta gcccagctca tttagccggg tttcgaaatc cggttcatgg
      121 tcgccgagtt cgaacgccgc cagtacgcgc ccgtagtcgg tgccatggct gcgatagtgg
      181 aacaaagaaa tgttccagtg cgtacccagc gtgttgagga agcgcagcag cgcgcccggt
      241 gattccggga attcgaagct gtagaggcgt tcctgcaacg gatgtgacgg acgcccgccg
      301 accatatagc gcacatgcaa cttcgccatt tcgtcgtcgg agagatccac cacgctgtag
      361 ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt cgaggccgcg gctcaggcgc
      421 acgccgacaa agatgcaggc gtttttggca tcggcaaaac ggtagttgaa ctcggtgacc
      481 gatcgcccgc caagcagttg gcagaatttg aggaagctgc ctttttcttc cggaatggtc
      541 accgccaaca acgcttcacg ctgttcgccc agttcgcagc gttccgagac gtagcgcagg
      601 ccgtggaagt tcacgttggc accggaaaga atatgcgcca gccgttcgcc gcgaatgttg
      661 tgctgggcga tatatttttt cattcccgcc agcgccagcg cgccagaggg ttccgccacc
      721 gcgcgcacat cttcgaataa atccttcatc gccgcacaga tcgcatcact atcgacggtg
      781 atgatgtcgt cgagatactc ctggcataaa cggaaggttt cgtcaccgat gcgttttacc
      841 gctacgcctt cagcaaatag ccctacgcgc ggcagatcaa ccggatgacc cgcatccagc
      901 gctgctttca ggcaggcgga gtcttccgct tctacggcga tcactttgat ttgtggcatc
      961 agttgtttga tcagcaccgc aacgcccgcc gccagaccgc cgccgccgac tggcacaaat
     1021 acgcggtcga gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc ttgcccggca
     1081 atcaccatcg gatggtcgaa cggcggcacc caggtgaacc cctgctgctg tgacagttcg
     1141 atcgctttag ctttcgcttc gtcaaagttc gcgccatgaa gcagcacttc gccgccgaat
     1201 ccacgtaccg catcgacttt gatgtcggcg gtggcggttg gcataacgat cagggccttc
     1261 acgcctaacc gcgcagaaga aaacgcgacg ccctgcgcgt ggttacccgc agaagcagtg
     1321 atcacgccgt gcgctttttg ttcttccgtc aggcccgcca tcatggcgta tgcgccgcgc
     1381 agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca ccagaatgac gttatcaaga
     1441 cgcgacgaca gtttttccat tttttgtagc ggcgtaacct gcgccgcctc gtaaaccggc
     1501 gcacgcagca ctgctcttaa atattcggca ccttccggag caccggacag gggttgcgag
     1561 tcagccatta ttaacccccc agtttcgatt tatcgcgcac cgcacctttg tcggcgctgg
     1621 ttgccaggct ggcgtaggcg cgcagggcaa aggaaacctg acgttcacgg tttttcggcg
     1681 tccaggcttt gtcaccccga gcttcctgcg cttcacgacg cgccgccagt tcggcatcgc
     1741 ttacctgtaa ctgaatgcca cggttcggga tgtcgatagc gatcaggtca ccatcttcaa
     1801 tcaggccaat gctgccgccg cttgccgctt ccggtgagac gtggccgatg gaaagaccag
     1861 aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca ggctttaccg agacccattg
     1921 atttcaggaa gctggttggg tagagcattt cctgcatgcc agggccgcct ttcgggcctt
     1981 catagcggat caccaccaca tcgcccgcga caactttgcc accgaggatc gcttctaccg
     2041 cgtcgtcctg gctttcgtac actttcgccg ggccggtgaa tttgaggatg ctgtcatcga
     2101 cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt accgtagagc accgccaggc
     2161 cgccgtcttt gctgtaggcg tgttccagcg agcggataca gccattggcg cgatcgtcat
     2221 cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt ggtacgaata cccgcagggc
     2281 cggcgcggaa catatttttt accgcgtcat cctgggtcag cataacgtcg tattgttcca
     2341 gcgtttgcgg caacgtcagg ccaagtacgt ttttcacatc acggttcagt aaccccgcgc
     2401 gatccagttc gccgagaata ccgataacac caccagcacg gtgaacatct tccatatggt
     2461 atttctgggt gctcggcgca actttacaca gctgtggaac cttgcgggaa agcttatcga
     2521 tatcactcat ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg tgaagtactg
     2581 tgttagtcga tccacccatc gcgatatcca gcgtcatggc gttttcaaac gccgccttac
     2641 tggcgatatt acgcggcagt gcactttcgt cgttttgctc gtaataacgt ttggtcaatt
     2701 caacaatgcg tttaccagca ttaaggaaca gctgcttacg gtcggcgtgg gttgccagca
     2761 gcgagccgtt gcccggctgc gacaggccca gcgcttcggt caggcagttc attgagttag
     2821 cggtaaacat cccggagcag gaaccgcagg tcggacacgc ggaacgttca acctgatcgc
     2881 tctgggagtc agatactttc gggtctgcgc cctggatcat cgcatcaacc agatcgagct
     2941 tgatgatctg atcggaaagt ttggttttcc cggcctccat cgggccgccg gaaacaaaga
     3001 tcaccggaat attcaggcgc agggaagcca tcagcatccc cggggtgatt ttgtcgcagt
     3061 tagagatgca gaccatggcg tcggcgcagt gggcgttgac catatactca acggaatcag
     3121 cgatcagttc gcgagatggc agtgaataaa gcatcccccc gtggcccatg gcaatcccat
     3181 catccaccgc aatggtgttg aactctttgg caacgccgcc agccgcttca atttgttcgg
     3241 cgaccagttt accgagatcg cgcagatgga cgtgacccgg tacaaattgg gtgaacgagt
     3301 tcacaaccgc gataatcggc ttgccaaaat cggcgtcggt cattccggtg gcgcgccaca
     3361 gcgcacgagc acccgccata ttacgaccat gagtggtggt ggcggaacgg tacttaggca
     3421 tactttattt actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg tcccattttt
     3481 tgtatttatt gattaacttg atctaaccag ccccatttat cttcggtttc gccagtgaag
     3541 aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc cacaacggcc ttcgccaacc
     3601 tgaataccgt ctacgctgcg cactggcgtg atttctgccg cagtaccgga cataaacact
     3661 tcatccgcca ggtacaggga ttcgcgcgac agaacctgct cacgcacttc aattcccagc
     3721 tctttcgcca gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga ggaggtgaac
     3781 ggtggggtga acagcacacc atctttcact tcaaacaggt tttcgcctgc gccttcagag
     3841 atataaccgt tcacatccag cgcgatacct tcctgataac cgtggcggcg cgcttcgcta
     3901 cccaccagca gggaagagag gtagttacca ccggcttttg ccgcggttgg gatggtgttt
     3961 ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc cctgctccag cgcttctgcg
     4021 cccagatacg ctccccacgg gaaagcagcg ataatcacgt cggttgagta tcccgctggc
     4081 gggtttactc ccatgccaac atcaccgacg aagatcagcg gacggatata ggcgctggtg
     4141 agattatttt tgcggatcac gtcacgacaa gcttccatca gctcatcaat gctctgcgaa
     4201 accgggaagc gatagatttt ggcggagtca tgcagacgct gcatatgctc acgatggcgg
     4261 aatacaaccg gtcctttgtg cgagtcgtag caacggatgc cttcaaaaac cgaagtgcca
     4321 tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac catctcccca
     4381 ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta tattcctttt gcgctcaggc
     4441 gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga cgtccaccag
     4501 tttatttaac tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg tcaattcgat
     4561 atttatattt tgtgcatcgc tggcggcggc catattcatt gagcagacgt ggaaaccacg
     4621 atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga ttgaagcgag ccgatacatt
     4681 gacctgatgt tgcatcatga taatttctcc aacatttctg aattactggc accaggcggc
     4741 accagcggcc agacgttctc aagttcgtcg attgagacat gaagcaggta tggcccatca
     4801 ctgttcagca tggtgtcgag tgccgcttca acctggtctt tacgggtgat gtgttggcca
     4861 gggatgccga aggcgctggc taacatgaga aaatcggggt tatcggtaag ggtggtttcg
     4921 ctatatcgtt cctggaaaaa cagttgctgc cattgtcgaa ccatccctaa ccgttggtta
     4981 tcgagtaaga cgattttcaa cggtaactgc ttacgtttta cggtgcccag ctcttgtaca
     5041 ttcatcatga aagagccgtc accggagata cagacgacgg tatcgttcgg tcgcgcgact
     5101 tgcgcgccaa ccgccgccgg taaaccgaaa cccatggtgc ctaagccgct ggaggtgatg
     5161 aaattttccg ggcgagtatg ggcgatgtgc tgcgcagccc acatctggtg ctgccccaca
     5221 tctgtggtca cgacgcaatc cgcaggttta cgatccgaca gttgttttaa caacaatggc
     5281 gcgtagatag cgtcaccggg atggtcgtaa cgccaggcat gttcatcacg cagctgcgcg
     5341 cagtgtagct gccagtcatt gatatttaac ggctgctgta atgctggtaa cagagcattt
     5401 aaatcacctt gtaatgccac atgtgcctga cgcagcttgt tcatttctgc cgggtcgata
     5461 tccatatgga taacactggc gtgtggtgcg aaggtgttca gtttgccggt cacccggtca
     5521 tcaaaacgtg cacccacggc gatcagcaag tcgcactcct gcaccgcgaa gttcgccgct
     5581 ttggtgccat gcattcccag catgcccaga tagtacggat aatctgcttc aactgcgccc
     5641 agccctttca gcgtgcaggt ggcaggcatt tttgtggtag cgagaaattc tcgtaaagca
     5701 ggaactgcct gcgccatacc cacgccacca ccaacgtaca gcatcggttt ttgcgctttt
     5761 gccagcatct ggcgcacttg ctcgacttcg gcatgtggga aagtcacttc gttttcaacg
     5821 gtggtgaacc acggttccag gtcgccgctg gctaactgga tatcttttgg gatatcgacc
     5881 agaaccggac caggacgacc tgagctggca acgtcgaatg cttcagccat aatgcgcggc
     5941 aactcttcca gcgactgcac cagaaagctg tgcttggtac aggctaacga caatcccagg
     6001 acatccactt cctgaaatgc gtcagtgccg ataaacggtg cggacacttg accggtgatg
     6061 gcaacaacag ggatagaatc taacagtgcg tccgcaagcc cggttatcag gttggttgcg
     6121 cccggaccag acgtggcgat acatacgcca gttttgccgg tagcacgagc ataaccgata
     6181 gccgccattg ccgcaccctg ctcatgtcgg cacagcaagt gctccacgcc gccgtcatac
     6241 aatgcatcgt aaaccggcat aattgcgcca cccggataac cgaaaacggt gttcacaccc
     6301 tgtgcccgca acgcatgtac cacccactgt gcgccattca tagttagttc cccgtcctga
     6361 atcttgagaa acagaatttt gtgctgttat tcattgtctg ctcctcggtt atgtttttaa
     6421 ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa ggcttgatct
     6481 ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca ccaccacgct
     6541 aatcacgacc aagctaatca ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg
     6601 ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa tagcacaaga tattttttgg
     6661 ccgtttttta tgatggcttt aataatttca atagaaattg ttgtttttta aaaagaaaat
     6721 agtgagtgaa aaaaattcaa tttcagtacg aatgttgttc cctccctcgt agttcatcct
     6781 ttgtccgctg catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca
     6841 caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct
     6901 tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc atacccgcgc
     6961 agccctggga gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc
     7021 cggcttaacg atggtgggct taccagaaac aactgtaaaa gaagctcgcg atcgcgtgcg
     7081 cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaacctggc
     7141 tccagccgat ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct
     7201 ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact
     7261 ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc tccagtgcaa ctgaagctat
     7321 taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa
     7381 cggtgaagga tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa
     7441 gcacgctctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct
     7501 cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg gaaattaccg ccgctggcgg
     7561 gcacaacctt ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat
     7621 taatggcctg ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag
     7681 tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcaccgca
     7741 tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat
     7801 ttcgttggcg cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac
     7861 actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc
     7921 caaaataacc tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg
     7981 acattatcag ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg
     8041 gctctcgggg ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc
     8101 cggcattttg agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt
     8161 aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag
     8221 tccggaaata cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaaggaac
     8281 actgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag ttgcacgaac
     8341 cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag
     8401 ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg
     8461 catttcgccc tttttattaa tcatcagaat cggtgtagtc ttcagcacct tcaacctgcg
     8521 gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg
     8581 accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga
     8641 gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac
     8701 catgacgctc aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac
     8761 gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc
     8821 acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga
     8881 gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc actgtggata
     8941 cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg
     9001 aatcgctcta tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac
     9061 tgggtgtgaa ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa
     9121 aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg
     9181 cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcattg tgggaatgta
     9241 tgcttaatca gtggctggga cgtttgtatc aaaatcagga tgcccataca ggcttacagt
     9301 tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg
     9361 atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctgggat
     9421 atttcacttt agcgctttat accagtgccc cttcaaaact aaagggagat cttaattatc
     9481 tgcgacttga gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg
     9541 aagtgcccat tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta
     9601 atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg
     9661 tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa
     9721 atacgttgat tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatatgaat
     9781 ggctggcaag gatgccggta gaaggattta cttcggagag ggttatttca gataaaaaaa
     9841 atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca
     9901 cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa
     9961 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
    10021 taccgctcca ccgaattctt ttacaaccac cggttttatg accggctt
//
LOCUS       DAFAVF010000153        10059 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFAVF010000153 DAFAVF010000000
VERSION     DAFAVF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.153"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            510..1487
                     /gene="csiD"
                     /locus_tag="LGK42_003672"
     CDS             510..1487
                     /gene="csiD"
                     /locus_tag="LGK42_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBK2659867.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1507..2775
                     /gene="lhgO"
                     /locus_tag="LGK42_003673"
     CDS             1507..2775
                     /gene="lhgO"
                     /locus_tag="LGK42_003673"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBK2659868.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERL
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2798..4246
                     /gene="gabD"
                     /locus_tag="LGK42_003674"
     CDS             2798..4246
                     /gene="gabD"
                     /locus_tag="LGK42_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBK2659869.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4260..5540
                     /gene="gabT"
                     /locus_tag="LGK42_003675"
     CDS             4260..5540
                     /gene="gabT"
                     /locus_tag="LGK42_003675"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBK2659870.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAGIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIINQCFAEAKQ"
     gene            5777..7177
                     /gene="gabP"
                     /locus_tag="LGK42_003676"
     CDS             5777..7177
                     /gene="gabP"
                     /locus_tag="LGK42_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBK2659871.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7198..7860
                     /gene="csiR"
                     /locus_tag="LGK42_003677"
     CDS             7198..7860
                     /gene="csiR"
                     /locus_tag="LGK42_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBK2659872.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7861..8310)
                     /gene="kbp"
                     /locus_tag="LGK42_003678"
     CDS             complement(7861..8310)
                     /gene="kbp"
                     /locus_tag="LGK42_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBK2659873.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8394..8552)
                     /locus_tag="LGK42_003679"
     CDS             complement(8394..8552)
                     /locus_tag="LGK42_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBK2659874.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8735..9034
                     /locus_tag="LGK42_003680"
     CDS             8735..9034
                     /locus_tag="LGK42_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2659875.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9044..9568
                     /gene="ygaP"
                     /locus_tag="LGK42_003681"
     CDS             9044..9568
                     /gene="ygaP"
                     /locus_tag="LGK42_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBK2659876.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLIAGISGFCG
                     MARLLDKMPWNQQA"
     gene            complement(9615..10019)
                     /gene="stpA"
                     /locus_tag="LGK42_003682"
     CDS             complement(9615..10019)
                     /gene="stpA"
                     /locus_tag="LGK42_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBK2659877.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2337 a         2580 c         2726 g         2416 t
ORIGIN      
        1 tacaaaaaca tggggagtaa tagcgttaac attattatta ataatacccg aaaaattatc
       61 ccccctggta aagtatttac gcttagaggc ggaactctaa atatcaatat tcctgggcgt
      121 agcgctcttc ttttagggaa aacaggagaa ccgccgaact atctctattt ataatttaat
      181 gttatatctg ccccgataaa acggggcaga taatatgttt agtttactaa cggtcatttt
      241 gcagtgaagc catttactgt tttttatcga ccagataatc tgttctctaa tgttaactcc
      301 ccctaacctg ttgctttagt tattcatttc ctgtctcact ttgccttaat accctacgtt
      361 aaatgttact aatttgttgc ttttgatcac aataagaaaa caatatgtcg cttttgtgcg
      421 catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat cgccatgtca
      481 ccctacatct cataagagga tcgcttctga tgaatgcact gaccgccgta caaaataacg
      541 ctgtcgattc aggccaggac tatagcggat tcactctcat cccgtcggcg caatccccgc
      601 gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa caggttgccg
      661 aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc
      721 ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg ctaaaccgcg
      781 ctgaaggtgc gctgttgatc aatgcggtgg gtatcgatga tgtggcgcag gcagatgaga
      841 tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga
      901 gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac agctatctgc
      961 gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa gagatcaccg
     1021 attacgtgct gatgatgaaa atcgatgagc aaaacatgca gggtggaaat tcgttgctgc
     1081 tgcatctcga tgactgggaa catctggacc actatttccg ccatccactg gcgcgtcgcc
     1141 cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc catccagtgt
     1201 tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc gtccagccaa
     1261 aagattttga agaaggcgtg tggttgagcg agctttccga tgccattgaa accagcaaag
     1321 gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac ctgttctggc
     1381 tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg atgcgtcagc
     1441 gtggctattt cgcttacgcc actcaccact accagacgca tcagtaagcg caaaggaatt
     1501 gagcggatgt atgattttgt gattattggc ggcggcatca tcggcatgtc gaccgccatg
     1561 caactgattg atgtttatcc ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg
     1621 gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggttta ttacacgccc
     1681 ggtagcctga aggcgcagtt ttgcctggcg ggaaaccgcg ccaccaaagc cttttgcgat
     1741 caaaacggca ttcgctatga caactgcggc aagatgctgg tcgccacctc tgaactcgaa
     1801 atggaacggt tgcgcgcgtt gtgggaacgc acggcggcaa acggtatcga gcgcgagtgg
     1861 ttaaacgcca tggaactgcg cgagcgcgaa ccgaatatca ccgggcttgg cggcattttt
     1921 gtgccatcca gcggcattgt cagctaccgc aacgtgacgg cggcaatggc aaaaatcttc
     1981 caggccagag gcggtgagat tatctataac gccgaagtca gcggcctcag tgagcataaa
     2041 aacggcgtgg tgatacgtac ccgccagggc ggcgaatatg aagcatcgac gctgattagc
     2101 tgttccgggc tgatggctga ccggctggtg aaaatgctcg gcctcgaacc gggctttatt
     2161 atctgcccgt tccgcggcga gtatttccgc cttgcgccgg agcataacca gattgttaac
     2221 catctgattt accctattcc cgacccggca atgccgtttt tgggcgtgca tctcacccga
     2281 atgatcgatg gcagcgtgac cgtcgggcca aacgcggtgc tggcttttaa acgcgaaggc
     2341 taccgcaagc gcgacttctc gtttagcgac acgctggaaa ttttaggctc gtcggggatt
     2401 cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc
     2461 aaaagcggct atctgcggct ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc
     2521 cagccctggc ccgccggtgt gcgggcgcag gcggtatcgc cggatggcaa gttgattgac
     2581 gattttctgt ttgtcaccac cccgcgcacg atccacacct gcaatgcgcc ctccccggca
     2641 gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac gctgttggca
     2701 agccagagta accccggacg cacgctgcga gcggcacgta gtgtggatgc cttacacgcc
     2761 gcatttaatc aataaccttt gaaaacagga cgtaacaatg aaacttaacg acagtaactt
     2821 attccgccag caggcgttga ttaacgggga atggctggac gccaacaatg gcgaggtcat
     2881 cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtacccaaaa tgggcgctga
     2941 tgaaacccgc gccgctatcg acgccgccaa ccgcgctctg cccgcctggc gtgcgctcac
     3001 cgccaaagaa cgcgccaaca ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga
     3061 cgatttagcg cgtctgatga ccctcgaaca gggtaaaccg ctggctgaag cgaaaggtga
     3121 aatcagctac gccgcctcct ttattgagtg gtttgctgaa gaaggcaaac gcatttatgg
     3181 cgacaccatt cccggtcatc aggccgataa acgcctgatt gttatcaagc agccgattgg
     3241 cgttaccgcc gccatcacgc cgtggaactt cccggcggcg atgattaccc gtaaagccgg
     3301 tccggcgctg gcggcaggct gcacgatggt gctgaaaccc gccagtcaga cgccgttctc
     3361 tgcgctggcg ctggcggagc tggcgattcg cgcgggcatt ccggctgggg tatttaacgt
     3421 ggtcaccggt tcggcgggcg cagtcggtaa cgaactgacc agcaacccgc tggtgcgcaa
     3481 actgtcgttt accggttcga ccgaaattgg ccgccagtta atggaacaat gcgcgaaaga
     3541 catcaaaaaa gtgtcgctgg agctcggcgg caacgcgccg tttatcgtct ttgacgatgc
     3601 cgacctcgac aaagccgtgg aaggcgcgct ggcctcgaaa ttccgcaacg ccgggcaaac
     3661 ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg tatgaccgct ttgccgaaaa
     3721 attgcagcag gcggtgagca aactgcacat cggcgacggg ctggataaag gcgtcaccat
     3781 cgggccgctg atcgatgaaa aagcagtagc aaaagtggaa gagcatattg ccgatgcgct
     3841 ggagaaaggc gcgcgcgtgg tttgcggcgg taaagcacac gaacgtggcg gcaacttctt
     3901 ccagccgacc attctggtgg acgttccggc caacgctaaa gtgtcgaaag aagagacgtt
     3961 cggccccctc gccccgctgt tccgttttaa agatgaagcc gatgtgatcg cgcaagccaa
     4021 tgacaccgaa tttggtcttg ccgcctattt ctacgcccgt gatttaagcc gcgtcttccg
     4081 cgtgggcgaa gcgctggagt acggcatcgt cggcatcaat accgggatta tttccaatga
     4141 agtggccccg ttcggcggca tcaaagcctc gggtctgggt cgtgaaggtt cgaagtatgg
     4201 catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg agaacgcgaa
     4261 tgagcagcaa taaagagtta atgcagcgcc gcagtcaggc agttcctcgt ggcgttgggc
     4321 aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac gttgaaggcc
     4381 gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac ctgcatccga
     4441 aggtggttgc ggcggtggaa gcgcagttga aaaaactgtc gcacacctgc ttccaggtgc
     4501 tggcctacga gccgtatctg gagctgtgcg agattatgaa tcagaaggtg ccaggcgatt
     4561 tcgccaagaa aacgctgctg gttacgaccg gttccgaagc ggtggaaaac gcggtgaaaa
     4621 tcgcccgcgc cgccacaaaa cgtagcggca ccatcgcttt tagcggcgcg taccacgggc
     4681 gcacgcatta cacgctggcg ctgaccggca aggtgaatcc gtactctgcg ggcatggggc
     4741 tgatgcctgg gcacgtttat cgcgcgcttt atccttgccc gctgcacggc atcagtgaag
     4801 atgacgctat cgccagcatt caccgaattt ttaaaaatga cgctgcgccg gaagatatcg
     4861 ccgccatcgt gattgaaccg gttcagggcg aaggcggttt ctacgccgct acgcctgctt
     4921 ttatgcagcg tttacgcgcg ctgtgtgacg agcacgggat catgctgatt gccgatgaag
     4981 tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat ggagcagatg ggcgttgcgc
     5041 cggatctcac cacctttgcg aaatccatcg cgggcggctt cccgctggcg ggtgtcaccg
     5101 ggcgcgcgga agtgatggat gccgtcgctc caggcgggct gggcggcacc tatgccggta
     5161 acccgattgc ctgcgtggcg gcgctggaag tgttgaaggt gttcgagcag gaaaatctgc
     5221 tgcaaaaagc caacgatctg gggcagaagc tgaaagatgg actgctggcg attgccgaaa
     5281 aacacccgga gatcggcgac gtacgcgggc tgggagcgat gatcgccatc gagctgtttg
     5341 aagatggcga tcacagcaaa ccggacgcaa aactcaccgc cgggatcgtg gcgcgcgccc
     5401 gcgataaagg cctgattctt ctctcctgcg gcccgtatta caacgtgctg cgcatccttg
     5461 taccgctcac cattgaagac gctcagatcc gtcagggtct ggagatcatc aaccagtgtt
     5521 ttgctgaggc gaagcagtag caccgcacca tgccggaggc gacgctgcgc gtcttgtccg
     5581 gcctacggtg atccaggtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa
     5641 caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat
     5701 aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     5761 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa
     5821 atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     5881 ttcaagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc tgttcgccgg
     5941 attactggtg gttatgatta tgcggatgtt ggcggaaatg gcagttgcca cgcccgatac
     6001 cggttcgttt tccacctatg ccgacaaggc aattggccgc tgggcgggtt ataccatcgg
     6061 ctggctgtac tggtggtttt gggtactggt tatcccgctg gaagccaaca tcgccgctat
     6121 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
     6181 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
     6241 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
     6301 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
     6361 tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat
     6421 gggcgcagaa attgtcacca ttgccgccgc ggaatccgac acgccggaaa aacatattgt
     6481 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
     6541 cgtagtggcg ttaataccgt ggaatatgcc ggggctgaaa gccgttggtt cttatcgctc
     6601 ggtactggaa ttgctcaata ttccccatgc gaaattaatc atggactgcg tgatattact
     6661 ttccgtaacc agttgcctga actcggcgct gtataccgcc tcaaggatgc tctactcctt
     6721 aagccgtcgc ggagatgcgc ctgcggtaat gggtaaaatc aaccgcagta aaaccccgta
     6781 cgtggcggtg ttactctcca ccggcgcggc attcttaacg gtggtggtga actattacgc
     6841 gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
     6901 tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga
     6961 aattcgcttg cgaatgtggc tttatccgtg gctcacctgg ctggtcatcg gctttattac
     7021 ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg
     7081 cttattagcg atagggatta tctgtaccgt gccgattatg gcgcgctgga aaaagctggt
     7141 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
     7201 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
     7261 caaccggatg aaaaattacg aatgagtttg ctgacatcgc gttatgcact tggcgttggg
     7321 ccgttacggg aagctctttc gcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
     7381 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
     7441 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
     7501 gcagacgttc tcgcaaaagc gcatctgctg agtaagctgg aggcctgtga cgccagcgag
     7561 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc
     7621 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat
     7681 cgatttatct ggctgcggcg aacggtgctt tcggtagaaa tgctggagga taaacacgat
     7741 cagcaccaga ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta
     7801 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
     7861 ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat
     7921 cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga
     7981 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt
     8041 cgccgttttc acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag
     8101 gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc
     8161 aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac
     8221 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc
     8281 ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
     8341 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg
     8401 cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga
     8461 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag aggcggcaga
     8521 atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat
     8581 tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct gccagtttca
     8641 gcttacgctt ttactctcat ttacgctgct gaggctggta tatgttgcat ttttagttta
     8701 gaatttactt aatttagaaa atacttaaat aaatatgact gaactcgcgc aattacaggc
     8761 cagtgccgaa caggcagcgg ccttattgaa agcaatgagc catcctaaac gattgctgat
     8821 tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc gcattaccgg
     8881 actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag ggcttatcga
     8941 cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg taaatgccat
     9001 tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt tgacaaccat
     9061 ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg atattcgtga
     9121 cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat tatccgtgct
     9181 ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc actgccaggc
     9241 aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc ccgcagaaat
     9301 atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag tagcggtaaa
     9361 taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt
     9421 aatcggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa gtggctttgt
     9481 tggtgccggg ttactgattg caggaatcag cggtttttgt ggaatggcaa ggttgttaga
     9541 taagatgccg tggaaccaac aagcttgaga agcgacgccg gacgcgccct agcagcgaca
     9601 tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc cagcgcctga
     9661 gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc gttaacatca
     9721 gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcacgtgg tgcagcagca
     9781 gagctattac ccaataactc ttccgggtta attccgtcag ctttcatcag ctccagccag
     9841 gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc ttcttcacgt
     9901 ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc aatggagaat
     9961 tcgcgagcca tcgcacggag ggtgcgaatg ttatttaaac tttgtaacat tacggacata
    10021 ataaagaaaa ccttttaacg ccaaaacaaa aaagtattt
//
LOCUS       DAFAVF010000154         9860 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFAVF010000154 DAFAVF010000000
VERSION     DAFAVF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.154"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1032
                     /gene="pgaB"
                     /locus_tag="LGK42_003683"
     CDS             <1..1032
                     /gene="pgaB"
                     /locus_tag="LGK42_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBK2659878.1"
                     /translation="QQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNR
                     LLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQ
                     YHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGF
                     SGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQP
                     ESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSI
                     LELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEF
                     STAWYPKND"
     gene            1025..2350
                     /gene="pgaC"
                     /locus_tag="LGK42_003684"
     CDS             1025..2350
                     /gene="pgaC"
                     /locus_tag="LGK42_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HBK2659879.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            2352..2765
                     /gene="pgaD"
                     /locus_tag="LGK42_003685"
     CDS             2352..2765
                     /gene="pgaD"
                     /locus_tag="LGK42_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HBK2659880.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(2815..3603)
                     /gene="phoH"
                     /locus_tag="LGK42_003686"
     CDS             complement(2815..3603)
                     /gene="phoH"
                     /locus_tag="LGK42_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBK2659881.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4222..5493)
                     /gene="efeB"
                     /locus_tag="LGK42_003687"
     CDS             complement(4222..5493)
                     /gene="efeB"
                     /locus_tag="LGK42_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBK2659882.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(5499..6626)
                     /gene="efeO"
                     /locus_tag="LGK42_003688"
     CDS             complement(5499..6626)
                     /gene="efeO"
                     /locus_tag="LGK42_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBK2659883.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(6684..7514)
                     /locus_tag="LGK42_003689"
     CDS             complement(6684..7514)
                     /locus_tag="LGK42_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBK2659884.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(8056..9564)
                     /gene="putP"
                     /locus_tag="LGK42_003690"
     CDS             complement(8056..9564)
                     /gene="putP"
                     /locus_tag="LGK42_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBK2659885.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
BASE COUNT         2549 a         2612 c         2301 g         2398 t
ORIGIN      
        1 cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat gcaaatatca
       61 accgtgtatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa agaggtctgg
      121 tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc ctggcaatta
      181 cgtacccgct caggtgtaaa catctatgcg tggatgccgg tattaagctg ggatttagat
      241 cccacattaa cgcgagtaaa atacttacca acaggggaga aaaaagcaca aattcatcct
      301 gaacaatatc accgtctctc tcctttcgat gacagagtca gagcacaagt tggcatgtta
      361 tatgaagatc ttgccggaca tgctgctttt gatggcatat tgttccacga tgatgctttg
      421 ctttcagatt atgaagatgc cagtgcaccg gctatcacgg cttatcagca agcaggcttt
      481 agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt
      541 aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa agccattcgc
      601 ggtccacata ttaaaactgc acgaaatatt tttgcacttc cggtaataca acctgaaagt
      661 gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg gaccgctatt
      721 atggctatgc cttatctgga aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa
      781 ttgaccaatc aaattaaaaa catccctcag gctaaagaca aatctatttt agaattacag
      841 gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca actcgctcac
      901 tggatgagcc tattacaact gaatggagtg aaaaactatg gttattatcc cgacaatttt
      961 ctgcataacc aacctgaaat agaccttatt cgtcctgagt tttcaacagc ctggtatccg
     1021 aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat gcatacccct
     1081 atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct tctggccgtt
     1141 ttttatgtcc attatgtgga ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca
     1201 ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt ctatctccat
     1261 tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg ccgctttagc
     1321 acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag ataaaacccg
     1381 tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc atctggcgca
     1441 aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct
     1501 ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg gcggcatata ttgtggaacc
     1561 gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc gaacacgttc
     1621 taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt tgatcaagcg
     1681 aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg catttcgtcg
     1741 cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag atattgatat
     1801 tagctggaag ctgcagttga atcagtggac gattttttac gagccacggg cgctgtgctg
     1861 gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct gggctcaggg
     1921 cggtgcagaa gtattcctca aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat
     1981 gtggccgctg ttttttgaat actgcctgac gacaatatgg gccttcacct gcctggtcgg
     2041 tttcattatt tacgcagtcc aacttgccgg tgtaccgtta aatattgaat tgacacatat
     2101 cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc aatttattgt
     2161 cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt tctggattat
     2221 ttggttcccg gttattttct ggatgctgag cctggcaacg acattggtat catttacacg
     2281 agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc gcgggattct
     2341 gagaggttaa tatgaacaat ttaattatta cgacccgaca atcaccagta cgtttactgg
     2401 ttgattatgt tgccacaacc atcttgtgga cattatttgc gttgttcata ttcttattcg
     2461 ccatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga cttcagttct
     2521 attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg tacaataagc
     2581 tgcgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa gaatatgcag
     2641 agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac aggatgagcg
     2701 tacacttcac cagccagggg caaataaaaa tggttgtttc agaaaaagcg ctagtccggg
     2761 cataaacacc caaaacaaag cccggttcgc ccgggctctg caccgataat acacttaact
     2821 gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg
     2881 gacgatccca accatttcat cttcttcgaa acgttccagc gcgtcactta atccggagca
     2941 cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg acgataaccg tcacgttctc
     3001 cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg cctcgtcaag
     3061 aatgacgact gcattttcaa aggtacgtcc acgcatatag gcgaacggcg caatttccac
     3121 cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc gccggaccag
     3181 cacgtcgtag accgggcgaa aatagggagc aaacttttct gcgatatctc caggtaagaa
     3241 gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt cgacatcctt
     3301 atgtatcagg gcctctgccg cttttgctgc gctgatccag gtttttccgc acccggcttc
     3361 gcccgtggcg aatatcagct gcttactctc aatagccttc agatagtgca attgcgcttc
     3421 atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg gccataccaa tggcttctac
     3481 gccgcccatc tgcacaagcg aggtgaccga ttcttcttca cgctgcttat ggctgcgcga
     3541 atcccgtctc agcacacgtt ttgcctcacg acgagctttg atcactgctt tttgtcttcc
     3601 catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag cgcgattatg
     3661 cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg gataaaatgc
     3721 cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga
     3781 gaaaaataca gagtgacgaa gttgctaccg gaggagaaat ctccgcaagt ggtggagcgt
     3841 agcttaacaa gacattgtcc agaacaggac gttaccatcc gcgatctcca tagtgactga
     3901 ctatcactgc cgggaacttc cgctgctact taataattac aacagatctc gcattcattg
     3961 caacaatata ttcacttata tttaaccata aaacaccatt tcagtgacat tagtttctac
     4021 tggaaagatg acagagtgat gacagatatg aaaaaagctg tgtgctttca gcaggattcg
     4081 catcaggcat ctgcgcacga acgccggatg cggcgtgaac gccttatccg cctaccgaac
     4141 ccgcaccgat tgtaggcata agaagacgcg caagcatcgc atcaggcatc tgcgcaagaa
     4201 cgccttatcc gtctataaac attaaacccg caataacgcg cttccgagat aatcgttcgc
     4261 gtccttcact cccggcagcg caaaaaaata accgccgccg ataggtttaa cgtattcctc
     4321 cagcgcttcg ccattgagcc ttttttgtac agtcaggaag cctttttcca gatcgtgttg
     4381 gtaacagaca aacagtagcc ccatatccag ttgcccggag ttggtgacgc ccagtgaata
     4441 actgtagcca cgacgcagca tcaggctgga ctcactctcc gccgtgcggg gattcgccag
     4501 ccggatatgg ctgtccagcg caatcacctt cccttccgga tcgctggcgt aatcgggaac
     4561 atcatgctca tgctgcattc ccagcggcgc accggtttgc ttatcacgac caaaaatcgt
     4621 ctgctgttct ttcagcggag ttctgtccca aaattccact cgaaactgaa tcaagcgtac
     4681 tgcctgatag ctgccaccga ttgtccacgc tggctcctgc tgatctgccg ttacccacac
     4741 cactttttgc atcaacttat cattctggct atcgggattg gcagtgccgt ctttgaaacc
     4801 cagcaaatta atcggcgtct ctttgccttt actacgcgcc gcgtgatcgg aaataaaccc
     4861 ttcccgcttc cagcgcacgc tgagcaaatc tggcgtatgt ttgatgatat cgcgcagcgc
     4921 atggatcacc gtgtcctggg tgttggcgca aatttgtagc aacacatcac catgacataa
     4981 cgccgcatcc agcgagtcgt tgggaaaacg cgtcatcttc tgcaactttt tcggcatctg
     5041 tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg tgatggtgag
     5101 attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca ggcgcggatt
     5161 tggcgtttct ggtgccgctc cgccctgagt cagaaaagca aaacgctgag tcaacaagcg
     5221 aaacaaccgt tcaagatcgg gtttatcgct ggcaagcaca tcaaacgcca ccagcatcat
     5281 tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca ccataaaacg gctgtttctc
     5341 attgcgtgca tccggtgaaa gcgtacccgg cgcactttgc gttttttgtg catgagcgac
     5401 cggacaactt cccgccagcg ccagcgcacc aatgcctttc agtaaacgtc ggcgtgacgg
     5461 ttcattcacg ccgtttttat cttcatactg cataatgctt aatccaatcc cagcacaccg
     5521 cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc attccggtcg
     5581 gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag tacggtattt cgccagaatg
     5641 gtatcgacct ttttaaagtt ggcatcgact tttgccaaca gttccgggtt ggctttttgc
     5701 agttgtggac gcagcagatc gacaattttc tgcgagcctt caacgttagc ctggaaatcc
     5761 cacagatcgg tgtggctgta gcgatcttct tcaccgctga ttttgctggc tgccacttcc
     5821 tcaatcagtc cggctgcacc gccgaccact tttgaaggtg ggaaagccag ttcactgatg
     5881 cgtttttgca aatcgaccac atcggtataa agctggtcag cgtactgatc catgcctttg
     5941 gtggtgttgt cgccaaacaa tgctttttcc agacggtgga aaccggtgaa tttcggatcg
     6001 gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag atcggagaac
     6061 agttcagcaa tcggttcgat gcgctcatag tgctggcgcg tcggcgcata cagtgctttc
     6121 gctttttcga tatcgcctgc tttaatagcg tcagtaaagg ctttcgtgtc ggtcaccagc
     6181 tgcgtggttt ccgccatgac atacgcttta tatgcagtaa ttgcaccacc aagacttaac
     6241 agcgcatcac tttgcgccgc atccgccgtt gcctcacctt tgacgatcaa cttccctttc
     6301 gggttagtca gcagaccgca ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt
     6361 ttctggctaa agccaggggc gatattttcc cgctcttcca ccaccatcac gcctttgagg
     6421 atctcccact ccagcgcctt ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg
     6481 ttaaccgtaa tggtcatcgg ttcgcactgc ttatcggtca cggtcacctt gacctgcggc
     6541 acattagcgg cgttagccat aaaagcagaa gagaacagcg cagccacgct caactgcaat
     6601 gcgttacggc ggaagttaat ggtcatgacg agactatccc tttaaagtat gttgtaacta
     6661 agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg ctgtcgcccc tgcgcgtggt
     6721 ggcagagcaa atgccaccag cgccgggatg agataaataa accagacggc gacttcgctg
     6781 acgctcggcg cttcctgata gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga
     6841 gttgagagca ccgcactcat atcgaaggcg atttcctgaa agtggttcca caatccggct
     6901 tcatgaaatg cgcgaatggc accagccgcc agccctgcgg cgacgaagag aataaacagg
     6961 ctggtccatt taaaaaatgc accaagattg aggcgaatac cgccccagta gagcaggaag
     7021 cctagcacca cggcagtagc aagaccgagc attgcaccca gcggcggcca gatcccgaca
     7081 tcttgttgaa atgccgccag caggaaaaag accgactcca gcccttccct tgcaacggca
     7141 aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg
     7201 actgcctgct ccagttgtac tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc
     7261 caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc ctgctctttt
     7321 tgcggaaatt cgccggtggt ttcgttaatg aagatcccca agcccaggca caacgcagcg
     7381 gcaagcaaca cgccaatcca catcacacca atccatcggc ctcgctgggt acgcttaaga
     7441 tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga
     7501 aacggaacaa acatgccacc cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa
     7561 agtaaatcgc agtgatagcg attatcatta cggtaaagaa aaatacaagc ggaatgttta
     7621 taaaatgatg ttgaaatgta aacaaagaga ggtaatgcca ggggatataa acgattattc
     7681 caggtacaaa gatgggattg tttacaacga gccatgtgtt cttgccggat gccgcgtaaa
     7741 cgccgtatcc agccgacaaa accgtcctga ctcaatacat caaaacttcc atgtaaaact
     7801 gataagcggg tgtatcaagc aatttcgtcc atcctcgatt atcaaatcta actttttgcc
     7861 cggaacataa taacagctcg ggtgttggtg gcgtcaaaat cgctgaagcg ttccccgaag
     7921 gccgggacag cagggggccg cggcgattgg ccgccccctg cgcgctccct gcaccattca
     7981 atttatatgg cgaggcacat taagggagcg caacccgctg ctgaagccaa acaaaaccgc
     8041 cgcaggctaa gtcccttagc tttcctgcaa ccgtgacggc ggagccgaat gatagtgcgc
     8101 atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc gctttaccca gcaaactaaa
     8161 cactacaatc ccgatactgc cgaagatgaa gcccggaata atttcgtaga gtcccagcca
     8221 gccgaactgc ttccagacga taaccgtcag cgcaccgatg atcatccccg ccagcgcacc
     8281 gttgcgcgtc atgcgtgacc acatcaccga gaacagcacc accggaccaa acgccgcgcc
     8341 aaagcctgcc cacgcgtagc tcactaagcc cagcacgcgg ttttccgggt ttgccgccag
     8401 cgcaatcgcc accagcgcca ccaccagcac catcacacgc cctacccaca ccagctcttt
     8461 ctggctggca tgtttacgca gaaacgcttt gtataaatct tcggtaatcg cactggagca
     8521 caccagcagc tggcaactta aggttgacat taccgccgcc agaattgccg acagcagaat
     8581 cccggcaatc cacgggttaa acagaatttg cgccagttcg ataaacacgc gctcagcatt
     8641 ctggtttacc gcacctgcta ccgccgggtg ctcattaaag taagcaatac caaagaagcc
     8701 gacagctacc gcccctgcca ggcagaggat catccaggtc atactgatac gacgcgcatg
     8761 gacaatgctg tggtgagagt ctgccgccat aaaacgcgcc aggatgtgcg gctgcccgaa
     8821 gtaacccagc ccccaaccca tcagtgagat aatggcgaca aagttcagcc ctttgagcat
     8881 atcaacgttt tcgatgctct tttgtttgat cacttccagc gagtcaccaa agccaccgac
     8941 actgataatg acgataaccg gcgtcaggat cagggcaaaa atcatcaggc tggcctgtac
     9001 agtgtcagtc cagctcaccg cgaggaaacc gccaataaag gtgtaaagga tcgtcgccgc
     9061 agctcccgcc cacagagccg tttcgtagct catgccaaag gtactttcaa acagacgcgc
     9121 gcctgccaca atgcccgaag cgcaataaat ggtgaagaac agcaaaatca ccagcgcgga
     9181 gataatgcgc aaaatgcggc ttttatcttc aaagcgcccg gtgaaataat ccggcaatgt
     9241 taaggcgtta ttgttgtatt cggtatgcac acgcaaccgc cccgccacca gcttccagtt
     9301 gatccacgcg cctaatgtca ggccaatggc gatccaactt tcggaaatcc cggaaagaaa
     9361 aaccgcgccc ggcaacccca ttaacagcca gccgctcata tccgacgcac ccgccgataa
     9421 tgccgtcacg aatggcccaa ggctacgacc gcccagaata tagtcgtcaa agtttttcgt
     9481 tgatcgccag gcgataaacc caatcaatat catgccaaag atatagacac aaaatgtcac
     9541 caacatcggt gtgctaatag ccatctaaag tctccaaaaa attattatcg gcaatgtcga
     9601 aacttgccgt tatatctgcc accggaacgg ggtaacagag tttatgtttt accagggcga
     9661 ccgtatcctg ccggaagcgc tggttattca caatcgattt aacacaccat ttacattaaa
     9721 ttttagtgct cagcgacact atttttcatc aggttgcact ctctcacatt ttttgcggtt
     9781 gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct gagttatttt tttaatccct
     9841 gtcatatcga tttcttttat
//
LOCUS       DAFAVF010000155         9821 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFAVF010000155 DAFAVF010000000
VERSION     DAFAVF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.156"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..150
                     /gene="nikA"
                     /locus_tag="LGK42_003691"
     CDS             <1..150
                     /gene="nikA"
                     /locus_tag="LGK42_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBK2659886.1"
                     /translation="AFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRK
                     QLMEG"
     gene            161..2860
                     /gene="nikB"
                     /locus_tag="LGK42_003692"
     CDS             161..2860
                     /gene="nikB"
                     /locus_tag="LGK42_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBK2659887.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG"
     gene            complement(2897..5188)
                     /locus_tag="LGK42_003693"
     CDS             complement(2897..5188)
                     /locus_tag="LGK42_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBK2659888.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(5181..6251)
                     /gene="trbB"
                     /locus_tag="LGK42_003694"
     CDS             complement(5181..6251)
                     /gene="trbB"
                     /locus_tag="LGK42_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBK2659889.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            complement(6270..7478)
                     /gene="trbA"
                     /locus_tag="LGK42_003695"
     CDS             complement(6270..7478)
                     /gene="trbA"
                     /locus_tag="LGK42_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBK2659890.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            7770..7922
                     /locus_tag="LGK42_003696"
     CDS             7770..7922
                     /locus_tag="LGK42_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBK2659891.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            complement(7994..8245)
                     /locus_tag="LGK42_003697"
     CDS             complement(7994..8245)
                     /locus_tag="LGK42_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659892.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(8904..9080)
                     /locus_tag="LGK42_003698"
     CDS             complement(8904..9080)
                     /locus_tag="LGK42_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659893.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            9472..9681
                     /locus_tag="LGK42_003699"
     CDS             9472..9681
                     /locus_tag="LGK42_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBK2659894.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
BASE COUNT         2379 a         2316 c         2546 g         2580 t
ORIGIN      
        1 gcttttctga aggagttaat gagactggga aggatgcaga agcatctgtt tgttcaggga
       61 aaaagaaccg gtgacaagga atacgcagaa gtgcttgttg ccataaccga acttacaaat
      121 acattgcgta aacagttaat ggaaggttga ggattctccg gtgaatgcag tcattccgaa
      181 aaagagaagg gacggcaagt cttcgttcga agacctggta tcctacgtct ctgtccggga
      241 tgacatgacc gatgaggaac tggatttatc ttcttcttcg caggctgaac aaccgcaccg
      301 aagccgtttc agtcgccttg ttgattatgc gacacgtctt cgaaatgagt cattcgtggc
      361 gctggttgat gtcatgaagg acggctgcga atgggtcaac ttttatggtg tcacctgctt
      421 tcataactgt acttctcttg aaactgccgc tgcagatatg gagtacattg cccggcaggc
      481 acactatgca aaagatgaca ctgatcctgt ttttcactac atcctttcct ggcagtcaca
      541 tgaaagcccg cgtccggaac agatttatga cagtgtacgt catacgctga aatcactcgg
      601 ccttgccgac catcagtatg tctctgccgt gcatactgat acagataatc tgcacgtcca
      661 tgtggctgtt aaccgggtac accctgaaac gggttatctg aaccggttat cctggagtca
      721 ggaaaaactc agtcgtgcct gccgtgagct tgaactgaag catggttttg ctccggataa
      781 cggttgctgg gtccatgcac cgggaaatcg tatcgttcgc aaaaccgccg ttgaacgtga
      841 tcgccagaac gcgtggacac gcgggaaaaa acaaactttt cgcgagtatg tcgcgcagac
      901 agcggtcgct ggtttacgca gtgaacctgt aaatgactgg ttatccctgc atcgtcgtct
      961 tgcggaagat ggtctgtacc tgtctcagat ggacggaaaa tttctggtga tggatggctg
     1021 ggatcgcaac agggaaggtg tacagcttga ctcgtttggt ccctcctggt gtgcagaaaa
     1081 actcatgaaa aaaatgggtg actacacgcc agtaccaaaa gacattttca gccaggtgga
     1141 agcaccagga cgctataatc cggacttcat tgccgctgat gttcgcccgg aaaaaatcgc
     1201 tgaaacagaa agtctgcagc agtatgcctg tcgtcatctt ggagaacgtc tgccggaaat
     1261 ggcacgggaa ggacggctgg aaaactgcca ggctattcac cgcacactgg ctgaagcggg
     1321 attatggatg cgtgttcagc atggtcacct ggttatctgt gacggttatg atcataacca
     1381 gactcccgtt cgtgctgaca gcgtatggtc gctgctgacg ctcgacaatg tgaatcaact
     1441 cgatggtggc tggcagcctg taccaacaga tattttccgc caggttacgc caacagaacg
     1501 cttccgtggt cgtcgtatgg agagctgtcc tgcgaccgat aaagaatggc accgtatgcg
     1561 tacaggcacg gggccgcagg gggctatcaa acgcgaactg ttttctgaca aagaaagtct
     1621 gtggggatac agcatcagcc attgtagccc tcagattgaa gaaatgatca cacagggtga
     1681 gtttacctgg cagcgttgtc atgagctgtt tgcacaacag ggactgatgc tacagaaaca
     1741 acatcacggg cttgttgtcg ttgatgcctt taatcatgag caaacgccgg tgaaggccag
     1801 cagcattcat cctgatctga cactggggcg tgcagagcca caggccggac cttttgtgag
     1861 tgccccagca gatttgtttg acagggtgca acctgaaagt cgctataacc cggagctggc
     1921 tgtcagtgac aggtacgggg tcagcagtaa acgtgaccca atgctgcgcc ggcagcgacg
     1981 tgaagccaga gctgaggccc gtgctgacct gcgtgcccgc tatctggcat ggcgtgaaca
     2041 atggcgtaaa ccagatctgc gttatgggga acgttgtcgg gaaattcatc aggcatgccg
     2101 tctgcggaag tcacacattc gtgcgcagta cgatgatccg gcattacgta agctgcatta
     2161 tcacattgca gaagttcagc gaatgcaggc attgatcagg ctgaaagaag acatcaggga
     2221 tgagcgacag aaacttattg ctgatgggaa atggtatcca ccctcttacc gtcagtgggt
     2281 cgaaattcag gctgctcagg gagacagggc tgctgtatcg cagctgagag gctgggatta
     2341 tcgcgatcgc cgtaaagaca ggtcacgcac aacgacgaca gaccgctgtg tcgtgctttg
     2401 tgaaccgggc ggaacgccgg tatacggtaa taccggtgat ctcgaggccc gtctgcagaa
     2461 gaacgggagt gttcgtttcc gtgaccgtcg gacgggagag tttgtctgta cggattatgg
     2521 tgacagggtt gttttccgta atcaccatga ccgcaatgcg ctggcagata aactggattt
     2581 gattgcaccg gtattattcg ggcgtgatcc acgcatgggt tttgaaccag aaggaaacga
     2641 caaacaattt aaccaggtct ttgctgaaat ggttgcctgg cataatgtga caggccgtac
     2701 cggtcacgaa gactacagga ttacgcgacc ggacgtggat catcaccgtg aaggaagtga
     2761 acgctactac cgcgattata tcgcggcgaa cagcaacaat gacgcatcac tgcctccgcc
     2821 ggagcaggat aaacgctggg aaccgccttc tccaggttaa aaaaaaggcc gcgccagaat
     2881 ccggcgcggc cactgttcag tacattccgt cccagtccgg acggttggtt tttttatgat
     2941 gaaacccgtt tacaggacgc cctgtcgggt ggttattgtc ctgtggaagc gttaacaggt
     3001 ttatacctgc tctttctgct ctcactgata tctgattttc ctcttttgtt tcaggtagtt
     3061 ctggtctgtt cagcgtgata tagcgaattc gtcgctcttc ccgtgtggtc gttttaagcg
     3121 tattcaccgc tgtttcccac aaggcacttt cacgtgcgtt gatatctgtg tcgtactctt
     3181 ccagcattgc catagccgcc tgacgtcctg caatgcgttg ctggaatgta tccacgatcg
     3241 tgtgcggctc tgtgcgaatt tttcgtcttt ttctggaggg ttttgcagtc aggacgccaa
     3301 tgatggcact gacattttcc ggtgacggta ttcgacgttg agtagtacgg ggaacaagac
     3361 ggcggagacg gtccaggcgt ggcgcatcca cgctgatata cctgtttata acaacgggat
     3421 cgctgctgca gcttttctca tcgtcaggga tgaagaatga tgctgatggt acggtttctc
     3481 cccggaaaat agaaaagaac tcgccaggat gaagttcaat caactcctga acatttatct
     3541 tgctttccat caggatgctg atttgtgggc tgtcaatcca tgattcacca aatatgccgt
     3601 cctgacgctg caggttgttc attctcgccc tggcttcttc accggcggcg cttttcagcg
     3661 ttctggccgt actaccctcg ctgacaatcc tgccggcaaa ttttgtcccc gtgttctgca
     3721 tcagtgttgc agtattcgtg gctgttgtct gtccttctat acgttcctgg tcctgtgcca
     3781 tcaaaatcag agcaaaatcc agtgaacgca cctgagtggc ttccacagca atacggtccg
     3841 tatagtatgc ccccacttca tcaaggaaac acagataagg gaaacgcccc ttatattttt
     3901 tgacttccag tgcatcggaa tcagttcctt cgaggcggta gcccaaatca cgggcgagaa
     3961 tcatactttt ctgggtgata aacattcgtc caagagcaga cgttgtatgc gctgatgtat
     4021 ccagagcggg gatcattacc atcagaatcc tgtcgctgtg aatgctgtca cggatatcga
     4081 tatcaccgga atcttcagca aaaatatccc cgaatgcttc cgtgaatgtg ctgaaagttt
     4141 cagaaaactg cccggagaga taagcatgtt gttttctggg ttcttccgtc caggctgatg
     4201 gtgtcctgac cagcgacagg tcgaaaccgg gaacgtcctg aaggtagtta cgtaacggtg
     4261 cgatagcttc ttccggccac tgatcatcga ggccacgaca gtaaagtttg gccatccctt
     4321 cgagagtcat atgttcacgg agcatctgca gcgacattgt tttgccttcg cgcacacacc
     4381 agaatttagt accaaatact agtgccttat tcatggcaat ggcccgggac tgccactcgc
     4441 ccccctggac gttctggggc agcattgact gcattgtctc cgctgtgaat gcctcagtgc
     4501 tgtaacagaa gggattccag gtatttgact gaggacgcgt tttttcacca ctcagaatga
     4561 tttcactgcg ggattttcct ccgttcatga agttaatgac ttccacatcg tcttcacgtc
     4621 caaagcgtct ggccagatac cagatcgttc ttgccgtatc attctgtgcc ttgccatcga
     4681 caagggtgaa tcctcttgcc cagcaaagtg ggttaatcgc ccaggcaaag atggtttccg
     4741 ttttaccgcc accggtggtg gcgaaaaaca ttatatggcg ggtcagatcg tccatactca
     4801 gccataactc cctgccaata tccctgacgc gttgataacc aacatagaat atgccgctgg
     4861 ccggtgattc cagaatgacc tcatactgga acagggttgg ccagaagctg aaaagacttc
     4921 ttttaatcat tctgtcctgt gacgggtcag cacattccag cgtcatgggc atacgaagag
     4981 ggcagcgcca tcgctgcaga ctaaatacca gcattgttat cagtgtgaac aacagacatg
     5041 ccggtaatgc aagtggccag ataaaactgg ccacaagact tgccaccagg caaaagccat
     5101 agatattgag actctggagt gcattccaga caggatttcc ccaggctgta cggtgtaaaa
     5161 gctcggggtt aacgcgatgt tcactcatgg ccagactcgc tgtcggtggt tttcaggaat
     5221 aaattaagcg tgccagtttc ctgcagaatt cctgtaggca gatgccagcc ggtgtcggac
     5281 agcacccatg gggtaccggc aaatccgaac ttacggaagg cgacattgtt aacatcaatc
     5341 gcagctctgg ctgctttcag gcatgtttca tctgcaggcg ttgtttcttc tgatggcgtc
     5401 atcattccgt tatctgcgga atacagtcgg tgccagccgg cagcacgttt ctgagcatct
     5461 ttttcacaca gcagtggtgc cacacggtcg gtggattcct ctccaccaat aactgaaacc
     5521 gggtagataa cgacattgaa gtcgctggcc aggcgtttta aggccggctc cagaagtctg
     5581 cagttaggac aggcaggatc gagaaatgcg aacagggttc gttcatgacc ggatgacagg
     5641 ttgatggtaa acatgccccg atcggcgttc cgcttcagaa cagtagccag attatgtcgc
     5701 gcagctgcgg cttccggatt tttgcttgtg attgttttct ccgggacctg gacgtctttt
     5761 ttcgggggaa ccgggagttg aatgccttca tttaatggca cagtgtgatt caggtgaggg
     5821 ggtagcagtc gtggctcacc tgctgtctgt actgcttcgg gcgttgtatc atcccagaat
     5881 acacgatcaa catagcggaa cagagagaag ccaatcgttg ccattacgaa cagcacaacg
     5941 ctggcgatta tgccacggaa caaaggtgtt ctgctgaagc ggcgttccat cagatattca
     6001 aggcgcaggc aggaatcaat agctgtaagt atctggtcag cgttatgctc ctgtttatca
     6061 atcaggacgg tttctctgtt acgggtgccc atggcgtcaa aggctatatg aatcagttga
     6121 ttatttcgtg ttaccagccc tgacagggca tcccgtggaa ggacggcaat aatttctgtc
     6181 ctgccatccg gccagattgc ctgcacggaa atatgttttg caatgcgtgt tgcaaagtac
     6241 tcgattgtca ttttcagttc tccggataat cagaattcgt tgtctgtagc aggtgtgcgg
     6301 ggcaggatgt cgtcggtgag ggaccagttg gtcaggatgc ggcgtttccg gtcgcgatct
     6361 ggttgtggtt catcccagat aagaccgaga ctgagcatat cccgccgtaa gccttccacc
     6421 gcttcatcaa cacagggttc agggcaggga agaccaaaac gcatggcttc ggcctctgca
     6481 cgtgcaacgg ccaccacgcc agcgccttca ataaatcctt tcgtggtatt ggcgcggtgt
     6541 aatgcataaa acagcgtcct gtccacgcct ttcagccaaa tccagttggg tggcgtaagc
     6601 cgcaggtcat gtgcatacag ccagaccagc ccactgcgga cgtagcgatg ttgtttcagc
     6661 cattgctgtg ctccgttgct tttaatcact ctctggaaag ctgatttggc cagtgaataa
     6721 acaggggtac agaatttccc actgtcccgt ttgcttttaa ttctgcatga gaggttcagt
     6781 gtatccatga gctttaatgc tgccttacga tcatccagga aatactgaag accaaatatg
     6841 gcaaagagcg ctttttcatg tggtgccatg tctttccagg acgttaaagg tttacccaac
     6901 tgggccatga aacatcgtct tgcagctgca acatcgagct gcatattggt gatgagagta
     6961 tgctgctcga caaatgcttc cggggtgaga gcaacccgcc gttcaggtcg tttgccacca
     7021 tgaaagagtt tttccggatc accatctgcc agtacagggg cgatagcagg ggacaacgaa
     7081 gcaaagatgt gtggcagtcg ggtaatatct actggtctgc gggtgaaacg actttgtccc
     7141 ggatgttgat accactccca caaacaccag agtgtcacag gtaagaggta catccacaga
     7201 atacctatgg tctgctccat gacattaacc cactggctat agcttatgtt ggcggcgttg
     7261 ttaccggtca tggcaagcag gttgtatcgt ggtgctgcat agttatgaaa tggtccccag
     7321 tcaaccagtc cccataaggt atgaagaatc aggcagctgg cgtaaaccac ttccggcaag
     7381 aataaccata taacgaatag cagcaggata agaaggacac caacagcgcc ccatatctgc
     7441 ataggatctt ctgcgacagg ctgtcgattg taagacatga caaagtctcc atttctggaa
     7501 tttaaatcaa acggaaaggt taaaaattta tttgcaggcg ataagagcta accagaggaa
     7561 ttagccgtgc gataaaagga ttgttgcaga tacgaaagtc gttagaatag ctgcgggcgc
     7621 ttgaggctgt atgccgaaag cgttttgtgg acggtataca gcagaaagcc cctggagatt
     7681 tttttatcaa tcaaccaagg gctctactgt aatgcctaga caacattata gtagcccgat
     7741 aaccgccgta aggcaatgga ggggctatga tgccacagcg aacgttttta atgatgttaa
     7801 tcgtcatctg tgtgacgatt ctgtgttttg tctggatggt gagggattcg ctttgcggac
     7861 tccggctcca gcagggaaac acagtgcttg tggcaacgtt agcctacgaa gttaaacgtt
     7921 aacgggcaac acggcggcag gttttctgcc gccgctttgt tgcgaaggct gacctcattg
     7981 cgccctttct tcttcaatga agcgtatccg gtattgtttc attttccctg aagccgatta
     8041 gctccagccg aatgcctttg ccatcggggg ccacgttctg catttcgcag ataccacgtc
     8101 gggtcatgag tgtgaaggct gtcattgcca gcatggctaa cgcgttttcc tcgcttccgc
     8161 tgcgtttctc gagagcatcg gcaatttctt ctatgctccc cacctgaaag caagagtcga
     8221 attctttact ccacttttct gacatcggat tttctctctg tatttatggg tagttatcga
     8281 actgaatgtg ccagtttttt caggagacgg caaagttgag atgattggtt ctgattcaga
     8341 gcatgagttt cctcggtgtg acgggcatgt cgggcggatt ttgagaaaca acagatgtaa
     8401 aaaaaccgac tggtgaggtc ggttttttta ctgttccgtt acagagtggg tggccctgac
     8461 cggaggaaat ctcttttcaa ctaacgttct tataatagcg agctgttcat ttgatagcaa
     8521 ccatccttat aatccgcagg ttagctctgt ctggcgacac ttctgccgtg atatccatcg
     8581 ggactatgga tgcaagattt taagtcctgg ctgaacaaac aggtggtgct gtgttcagtg
     8641 ctctggcagg aggaagtctc ttttcaacta acactgaaag tgccgttgtg acaggcaatg
     8701 taagagtaag caacggtgat ttcctccagt gatggagaag cggatgatta atctgttaat
     8761 catcgttctc agggcagtag ttgccgttgc aaacgcgctg attgctgttc tggaactgat
     8821 ccgggaactt gtcgactgat aagcctggaa acgtaactaa gggcagggag ttaccgcttc
     8881 ctgcccttta acaaaaagag atctcacgtt aaaaatttgt acccttcttt aacttccagt
     8941 gcctgctcct ttgactcaaa catcagtctt gttttcccgg gaacgcccca tgaaatgtat
     9001 tcaacatcta tccaccattt tccatattct ttatatggcc cggatattac ttttttgaca
     9061 aaggcgtcta ccagattcat tattttttcc tttttgtgtt gaaaataatt aatgccagag
     9121 cacagcgaaa ataatgaatg cgctggctgt gataattgcg attttctccc atatgatatt
     9181 tttctcatgg gggtttctac ggagattatt tcttcgcatt gagataccgg tattcatgtt
     9241 ttatcttgga cttttgtttt gttgtctgtc tggcaggctg ttctgcgccc gtttgtgaca
     9301 aggttagctt atggggttaa atggtcacgg acatcatgcg gacggttccc tgtcacatgc
     9361 catctgtttt tttcattcaa attgagagat attgctaagt tgagtcgctt gcgatagtgg
     9421 actcctgttt gtaagtgagt gccattgttt atttcatgaa gaggattgat tatgagcatg
     9481 cctcctgcga tagctaatac tttcttgttt gaaatgatga agagtaaatc aaaagacgtt
     9541 acacttgctg cgatctatgc attaggtgaa ggccggtgtc aggcggagaa cattactcgt
     9601 gaattacata gattgagtca aagtgatgat atggaaataa aaattgctgc gattaaagca
     9661 cttggtcgaa tttatcggta acggcataag ttatctgttg taatgaacaa aagccggctc
     9721 atttgaaccg gctgttatgg agggagctta tcttaaaata cttcggctac aggcatgcct
     9781 gcaatctgct cccactcctt acgcttctcc tgaattaggc g
//
LOCUS       DAFAVF010000156         9644 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFAVF010000156 DAFAVF010000000
VERSION     DAFAVF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.158"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            33..803
                     /gene="yqeC"
                     /locus_tag="LGK42_003700"
     CDS             33..803
                     /gene="yqeC"
                     /locus_tag="LGK42_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBK2659895.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            851..2476
                     /locus_tag="LGK42_003701"
     CDS             851..2476
                     /locus_tag="LGK42_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBK2659896.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(2517..3449)
                     /gene="arcC"
                     /locus_tag="LGK42_003702"
     CDS             complement(2517..3449)
                     /gene="arcC"
                     /locus_tag="LGK42_003702"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK2659897.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(3497..4882)
                     /gene="hyuA"
                     /locus_tag="LGK42_003703"
     CDS             complement(3497..4882)
                     /gene="hyuA"
                     /locus_tag="LGK42_003703"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBK2659898.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(4935..6146)
                     /locus_tag="LGK42_003704"
     CDS             complement(4935..6146)
                     /locus_tag="LGK42_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBK2659899.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6204..7400)
                     /gene="dpaL"
                     /locus_tag="LGK42_003705"
     CDS             complement(6204..7400)
                     /gene="dpaL"
                     /locus_tag="LGK42_003705"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBK2659900.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(7458..8645)
                     /gene="ygeW"
                     /locus_tag="LGK42_003706"
     CDS             complement(7458..8645)
                     /gene="ygeW"
                     /locus_tag="LGK42_003706"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBK2659901.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMFLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9124..>9644
                     /locus_tag="LGK42_003707"
     CDS             9124..>9644
                     /locus_tag="LGK42_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659902.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGV"
BASE COUNT         2266 a         2350 c         2522 g         2506 t
ORIGIN      
        1 gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat
       61 tagtcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg
      121 ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt
      181 taatcacgac gacgacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc
      241 gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca
      301 gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg
      361 tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt ggaatgccgt
      421 taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg
      481 tgatgggagg acatactttg ggcgcgaaag tgagcacaga aaatgtccat cgctggtcgc
      541 agtttgctga tattaccggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg
      601 cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct
      661 ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag ctccttcaac
      721 cgctgcaaca acacgacgta gaggcaatct ggctgggcga tatacaagaa catcctgcaa
      781 tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat
      841 tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt
      901 tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg cacaaatgtt
      961 agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt
     1021 gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc atggacgtat
     1081 ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat
     1141 cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc
     1201 gatagcccag agtgcggcgc tattaggatt tgatattgcc gttgccgata tttatcgcga
     1261 aagcctcaat cctgaactat tccctccatc aaccacgctt ctccatgctg agtcgtttgg
     1321 tgcagcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa
     1381 tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct
     1441 ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga
     1501 agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca
     1561 ggagatcgcc atcagcgtgt tggcagaaat attacaggtg aaaaataacg cgccgggtgg
     1621 gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg
     1681 agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct
     1741 ggaagtggaa aaaccgacag tgattcgttg tacggtggcg tttgcccagg ccgtgttcga
     1801 tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagctctg cggaagcgat
     1861 gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga
     1921 tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacgcg
     1981 ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag ggaaggattg
     2041 tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt actctggttg
     2101 tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat
     2161 ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga
     2221 gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt
     2281 gcgtggcttg ttgaacgacg gcctggcagt ggtcggtggt tttaaaattg gtgatatcga
     2341 tcctcgtggt gaaacggctg atttcaccag cgtttctgat aaagcccggg cgattggcgg
     2401 cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt
     2461 gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac
     2521 tgacaaatca ccgttccggt tttgccttca atcccctctt tcgctttgct caatacggta
     2581 atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc
     2641 agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac
     2701 aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca
     2761 gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct
     2821 ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac aggaatacca
     2881 ccaccgccaa cggtgatcac cacctggcct gcatctacca gagctttaac cgtttctttt
     2941 tcaataatat caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct
     3001 tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa
     3061 ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta
     3121 accagcgttg ctaccggttt attaatgccc cgagaaagca gttcttctct taaggcgttt
     3181 tgtagatcgt aaccaatata accctggctt aaggcaacgc agacagacat cggcagcatt
     3241 ggcgagtgcg cttcagtttt tgcagcagct tcaaaggcct ggttaatcat gcccacctgc
     3301 ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc aacaattgcc
     3361 tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg
     3421 cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat
     3481 agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac
     3541 ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg
     3601 ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt
     3661 ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat
     3721 cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg
     3781 cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa
     3841 acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc
     3901 ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt
     3961 caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg
     4021 gtgggctaag aatgaacttc atgccgtctt ctgtatcgta actgcgttcg tccaacagga
     4081 gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat
     4141 aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt
     4201 gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg
     4261 catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag
     4321 ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga cggcgtaatg
     4381 cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc
     4441 tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta atgtgctgga
     4501 tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct
     4561 ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg
     4621 ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga
     4681 tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa acgtaacagc
     4741 cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa
     4801 caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc
     4861 cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa
     4921 taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc gtacatcgcg
     4981 gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct
     5041 ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta
     5101 gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc
     5161 accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg
     5221 gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg
     5281 ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg
     5341 ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt
     5401 ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa
     5461 cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg
     5521 ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt
     5581 tccatacgac cgcgctgacc acggtatatc tggcagtcgg ttggttcggt actgaccaca
     5641 aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct
     5701 tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta
     5761 atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga
     5821 ccaccgatta gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg
     5881 ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg
     5941 atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc gactttttcc
     6001 atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact gggaatagca
     6061 accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt ttcaagaatc
     6121 agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc
     6181 agggagcagt tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact
     6241 tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac cacggcatct
     6301 ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg
     6361 agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac
     6421 ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg
     6481 gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccagtcct
     6541 gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga
     6601 taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctatag
     6661 acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga
     6721 acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt
     6781 gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt
     6841 accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag
     6901 ttcatatctg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga
     6961 gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgcgc tgcccacgcc
     7021 acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca
     7081 tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat
     7141 tgagcgatgg cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa tcgttttgat
     7201 tcgtctttga caagaatttt cttcacacca aaaaggtttg cgagatcgtc cagcgcacaa
     7261 agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg
     7321 ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc
     7381 ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag
     7441 ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc
     7501 agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc
     7561 cagctggctt ctttgtaggt tgcgatacgg tatttttcga atacgccttc agtcacttca
     7621 ccttctttac aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg
     7681 ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc
     7741 tgtgccagac actgtttttc cagtgctttt aagccttcgt gatcgttagc gcgcagcaat
     7801 tcggtacgct gttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgca
     7861 tctttgaacg cttcttccat gctggtgacc tgacggaagc taccgccaga tgctttagca
     7921 ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg atgggccagg
     7981 gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt
     8041 ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc
     8101 agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt cgggtggtca
     8161 atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg
     8221 tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag aaacatatcg
     8281 tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt
     8341 tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag gccgagcagg
     8401 ttaagcgcgg aagcataaga gaagcgggta cgggtggagt tgtcgcggaa tacggaaata
     8461 cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc ttttaatgct
     8521 gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt taacaaaaaa
     8581 tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc attaacagtt
     8641 ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca ctgtgacttg
     8701 ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt gccagaatta
     8761 tccacggggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc acaaattgat
     8821 gccagcttcc ggttgtttgt aaaatacgaa aacgtttggc tttttttatc gcgttattta
     8881 tttgctgcta agtatcagaa ataagataaa gggacaggct atctctcttc agtttgataa
     8941 actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca taatttcatt
     9001 agaaaatgat aaaaatctta tgtttttatc aaagccaatt tcgcctgagt taatatcatc
     9061 atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct ggaaggtaaa
     9121 gctatggagc ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac aattcagcgt
     9181 tttgccagaa tgcttgccag cgttttgcag cttgaggttg agatcgttga tgaaaacttg
     9241 tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt gagcggcaac
     9301 tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac acagtctcgc
     9361 ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgcgaaaa agcatttctg
     9421 ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat tgccgttacc
     9481 cacgagcaac aagagcatat cagtgataat ttacgcgaat tttctgatta tgttcgccat
     9541 atatccacca tttttgtttc gaaacttctg gaggatcagg ggccaggaga taacatcagt
     9601 aaaatattcg cgaccatgat cgataatatg gatcagggcg tatt
//
LOCUS       DAFAVF010000157         9624 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFAVF010000157 DAFAVF010000000
VERSION     DAFAVF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.159"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(290..1267)
                     /gene="epmA"
                     /locus_tag="LGK42_003708"
     CDS             complement(290..1267)
                     /gene="epmA"
                     /locus_tag="LGK42_003708"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBK2659903.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1592..3400
                     /gene="frdA"
                     /locus_tag="LGK42_003709"
     CDS             1592..3400
                     /gene="frdA"
                     /locus_tag="LGK42_003709"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBK2659904.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3393..4127
                     /gene="frdB"
                     /locus_tag="LGK42_003710"
     CDS             3393..4127
                     /gene="frdB"
                     /locus_tag="LGK42_003710"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBK2659905.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4138..4533
                     /gene="frdC"
                     /locus_tag="LGK42_003711"
     CDS             4138..4533
                     /gene="frdC"
                     /locus_tag="LGK42_003711"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBK2659906.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAEFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4544..4903
                     /gene="frdD"
                     /locus_tag="LGK42_003712"
     CDS             4544..4903
                     /gene="frdD"
                     /locus_tag="LGK42_003712"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBK2659907.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4966..6099
                     /locus_tag="LGK42_003713"
     CDS             4966..6099
                     /locus_tag="LGK42_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBK2659908.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6188..6721
                     /gene="blc"
                     /locus_tag="LGK42_003714"
     CDS             6188..6721
                     /gene="blc"
                     /locus_tag="LGK42_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBK2659909.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6718..7035)
                     /gene="sugE"
                     /locus_tag="LGK42_003715"
     CDS             complement(6718..7035)
                     /gene="sugE"
                     /locus_tag="LGK42_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBK2659910.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7210..7356)
                     /gene="ecnB"
                     /locus_tag="LGK42_003716"
     CDS             complement(7210..7356)
                     /gene="ecnB"
                     /locus_tag="LGK42_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBK2659911.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7467..7592)
                     /gene="ecnA"
                     /locus_tag="LGK42_003717"
     CDS             complement(7467..7592)
                     /gene="ecnA"
                     /locus_tag="LGK42_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBK2659912.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7644..8210)
                     /gene="efp"
                     /locus_tag="LGK42_003718"
     CDS             complement(7644..8210)
                     /gene="efp"
                     /locus_tag="LGK42_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK2659913.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8252..9280
                     /gene="epmB"
                     /locus_tag="LGK42_003719"
     CDS             8252..9280
                     /gene="epmB"
                     /locus_tag="LGK42_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBK2659914.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2429 a         2478 c         2454 g         2263 t
ORIGIN      
        1 aatccaaata cggaagtaaa gatcatcaat atgagtccta tcagactcat cttttttatc
       61 gtgtgaggca tgaaaccatc cttcatttca gcgcgtgtat aaaacgcgcc cactgaactg
      121 cactgcccaa cattgagcgg gcagcgtaaa aaccaataca gaattgatac ctggatgata
      181 tcaaatatcc agtcttaaat tgtggccttt aacggaaaaa tgaaaacata tgctacagaa
      241 tggcgcttat cacgccattc ttcgctgtta attcagtaat ttttcagaat tatgcccggt
      301 caacgctaaa ggcgatgact tcagccagtg tctccgcgcc cagcgccaac atcaccagac
      361 gatcaacacc taatgccacg ccggaacagt caggcatacc gactttcaag gcttcaatca
      421 gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg ttatcttgtt
      481 caaagcgttg ttgctgctca cgggcatccg tcaattcatg gaaaccattc gccagctcaa
      541 tacctttata ataaacctca aagcgttcag cgacccgatg atcttcggta ctgatttgcg
      601 ccagtgatgc ctggctggct ggaaagtggt acacaaaggt cggtttttct ttgccaatat
      661 ttggctctac gccaaaggta aacagcaatt gtagcagcgt gtcgcggtct tcttcggtat
      721 cagcaacatt gctcaaatcc agtttcgctg cgacttcccg cagttgcgtt ttgtcggcag
      781 agagcgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg ctttctgctg
      841 ccgggcagtc cagcacctgt tgtaagagat cgtccacctc gttcatcaac cggtacatat
      901 catagtgcgg tcgataccac tccagcatag tgaactcagg gttgtgataa cgccccatct
      961 cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccggca accagcaggc
     1021 gtttcatatg gtattccggg ctggtcatta accagagatt catcccctgc gaatgcccgg
     1081 ggccaacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc gcctggctca
     1141 tacaaggcgt ctccacctcc agcactccac gatcggcaaa gaaacgacgg atctccgcca
     1201 taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag gatgccgttt
     1261 cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc aacgcgacgg
     1321 cgagacaaat tttacgcagg aatcaaacag cggtgggcag tgactaaaaa aagcacgatc
     1381 tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct tgcgcactaa
     1441 aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg tacctataaa
     1501 ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg aagtacgggg
     1561 ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg atcttgccat
     1621 tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg caaatccaaa
     1681 tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg ttgctgcaga
     1741 agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact ttcacgatac
     1801 agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg tccaccactg
     1861 cccaaccgaa atgacccaac tggaactgtg gggatgccca tggagccgtc gcccggatgg
     1921 tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt tcgccgccga
     1981 taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat tcccgcagat
     2041 ccagcgtttt gacgaacatt tcgtgctgga tattctggtt gatgatggtc atgttcgcgg
     2101 cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta acgcggtcgt
     2161 tatggctact ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg gcatcgttac
     2221 cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca tggaattcgt
     2281 tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgaccgaag gctgccgcgg
     2341 tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt acggcatggg
     2401 cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc cacgcgacaa
     2461 agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca cgccgcgtgg
     2521 cgatgtggtt tatctcgacc tgcgtcacct cggcgagaaa aaactgcatg aacgtctgcc
     2581 gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag aaccgattcc
     2641 ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga actgtgaaac
     2701 ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc acggtgcaaa
     2761 ccgtctgggc tccaactccc tggcggaact ggtggtcttc ggccgtctgg ccggtgaaca
     2821 agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg aagcgcaggc
     2881 agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg aaaactgggc
     2941 gaagatccgc gacgaaatgg gtctggctat ggaagaaggt tgcggtatct accgtacgcc
     3001 ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaacgct tcaagcgcgt
     3061 gcgcatcacc gacacctcca gcgtgttcaa caccgacctg ctctacacca ttgaactggg
     3121 ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta aagagtcccg
     3181 cggcgcacac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca acttcctcaa
     3241 acacaccctc gccttccgcg atgctgatgg cacgactcgc ctggagtaca gcgacgtgaa
     3301 gattactacg ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg cagccgataa
     3361 ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac ctgaaaattg
     3421 aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc tatgaagtgc
     3481 cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac aacctggcac
     3541 cggacctgag ttaccgctgg tcctgccgta tggcgatttg tggttcctgc ggcatgatgg
     3601 ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc gacggtatga
     3661 aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat atgacccact
     3721 tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc accgcggatc
     3781 agggtactaa catccagacc ccggcgcaga tggcaaagta tcaccagttc tccggttgca
     3841 tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca gagttcatcg
     3901 gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga
     3961 aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact ttcgtgggct
     4021 attgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag cagggcaaag
     4081 tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga gtgcaacatg
     4141 acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa aaaattgccg
     4201 ttttatcgct tttacatgct gcgcgaaggc acggcggttc cggctgtgtg gttcagcatt
     4261 gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc ggaattcgtc
     4321 gactttttac aaaacccggt tatcgtgatc attaacctga tcactctggc ggcagctctg
     4381 ctgcatacca aaacctggtt tgaactggca ccgaaagcag ccaatatcat tgtaaaagac
     4441 gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt ggttgccacc
     4501 atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta atccaaatcc
     4561 aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc
     4621 aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccactgg ggctgtttcc
     4681 gggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt
     4741 attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc
     4801 gatgcacgat ctgaaaattc acgtgcctgc gggcaaatgg gttttctacg gtctggctgc
     4861 tatcctgaca gttgtcacgc tgattggtat cgttacaatc taacgtatcg ccaatgtaaa
     4921 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accttatgtt caaaacgacg
     4981 ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca acaaatcaac
     5041 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatggcg
     5101 gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc
     5161 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
     5221 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccaca
     5281 acaaaatact ggcctgaact taccgctaaa cagtggaatg ggatcacact attacatctc
     5341 gcaacctaca ctgctggcgg cctgccattg caggtgccgg atgaggtgaa atcctcaagc
     5401 gacttgctgc gcttctatca aaactggcag cctgcatggg cgccaggaac acaacgtctg
     5461 tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt
     5521 tttgagcagg cgatgcaaac acgtgtcttc cagccactca aactcaacca tacgtggatt
     5581 aatgtaccgc ccgcagaaga aaagaattac gcctggggat atcgcgaagg caaggcagtt
     5641 catgtttcgc caggggcgtt agatgctgaa acttatggtg tgaagtcgac cattgaagat
     5701 atggcctgct gggtacgaag caatatgaat ccccgtgata tcaacgacaa aacacttcag
     5761 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg
     5821 ggctgggaaa tgctggactg gccggtaaat cctgacagca tcattaacgg cagtggcaat
     5881 aaaattgcac tggcagcaca ccccgtaaaa gcgattacgc ccccaactcc tgcagtacgc
     5941 gcatcatggg tacataaaac aggggcaacc ggcggatttg gtagctatgt cgcgtttatt
     6001 ccagaaaaag agctgggtat agtgatgctg gctaacaaaa actatcccaa tccagcgaga
     6061 gtcgccgccg cctggcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt
     6121 tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
     6181 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
     6241 ctgcagttct cctacgccgc cgcgtggcgt aacagtagtg aataatttcg acgccaaacg
     6301 ttatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga
     6361 aaaagtcacc gcaacataca gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg
     6421 ctataaccct gacagaggaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
     6481 accaactcgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
     6541 tattgcactc gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct
     6601 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
     6661 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
     6721 gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg
     6781 catcgggtta gcggactcac cgagcagcac aatgccggtg atggccgcgc cgactgcgcc
     6841 aatacccgtc cacacggcat aagccgtccc taccggtaac gatttcatcg cccaggcaag
     6901 tagcgccata ctgactatca tcgccgtcac agtaataaca cttggcgtca aacgactaaa
     6961 gccgtgggta tatttcaggc caacggccca taccacttcc agcagaccag caataactaa
     7021 gataatccag gacatatcag gctccggaga attggggccg tccccggtga aaagaggcgt
     7081 ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt ttgccaggtg gcaggggaat
     7141 ttcaactatt acctttgtga ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac
     7201 cgttattgct tattgctgcg ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga
     7261 aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
     7321 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
     7381 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg atggaaatct
     7441 gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga gttgccgaga
     7501 tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca
     7561 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc
     7621 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
     7681 tccactttga tgacttcgcc gatttgtacg aacagcggaa ctttaaccac agcgccagta
     7741 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
     7801 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
     7861 agagttacga tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt
     7921 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
     7981 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
     8041 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
     8101 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct
     8161 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
     8221 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
     8281 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct
     8341 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
     8401 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
     8461 tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc
     8521 caccgacccg ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa
     8581 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
     8641 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg ctcttgagta
     8701 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
     8761 gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
     8821 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt
     8881 tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa
     8941 tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct
     9001 gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgc tggcaaacct
     9061 gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc tagataaagt
     9121 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
     9181 gctgacactg gtgtcgggtt atctggtgcc gaaactggcg cgagaaatcg gcggcgaacc
     9241 cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagtta
     9301 aacattagcc tgatgcgcta cgcttatcag gcctacattg tgcattgaaa ttgaatggat
     9361 ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca tccggcaaga gcaacaaacg
     9421 caagtacagc aacctgtcat taaactggga aatttaatct ttgcccgttg cataaccttt
     9481 aattattcat atacattgct gttgttactc tttatatagc caacaccaat aattaacctt
     9541 tcttttctgt ttatatagag tgagtacggc tatttatgtt attttaacaa ctattactac
     9601 caagcccatt tatctggctt ataa
//
LOCUS       DAFAVF010000158         9564 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFAVF010000158 DAFAVF010000000
VERSION     DAFAVF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.160"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..582)
                     /locus_tag="LGK42_003720"
     CDS             complement(<1..582)
                     /locus_tag="LGK42_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBK2659915.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPK"
     gene            848..2347
                     /gene="ansP"
                     /locus_tag="LGK42_003721"
     CDS             848..2347
                     /gene="ansP"
                     /locus_tag="LGK42_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBK2659916.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2462..3523)
                     /locus_tag="LGK42_003722"
     CDS             complement(2462..3523)
                     /locus_tag="LGK42_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBK2659917.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDASNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3765..5867
                     /locus_tag="LGK42_003723"
     CDS             3765..5867
                     /locus_tag="LGK42_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBK2659918.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5903..6568)
                     /gene="mcbR"
                     /locus_tag="LGK42_003724"
     CDS             complement(5903..6568)
                     /gene="mcbR"
                     /locus_tag="LGK42_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBK2659919.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6766..7803)
                     /gene="curA"
                     /locus_tag="LGK42_003725"
     CDS             complement(6766..7803)
                     /gene="curA"
                     /locus_tag="LGK42_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBK2659920.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            7984..8502
                     /gene="mddA"
                     /locus_tag="LGK42_003726"
     CDS             7984..8502
                     /gene="mddA"
                     /locus_tag="LGK42_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBK2659921.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8499..8948
                     /locus_tag="LGK42_003727"
     CDS             8499..8948
                     /locus_tag="LGK42_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBK2659922.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8949..9182)
                     /locus_tag="LGK42_003728"
     CDS             complement(8949..9182)
                     /locus_tag="LGK42_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBK2659923.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9268..9441)
                     /gene="ortT"
                     /locus_tag="LGK42_003729"
     CDS             complement(9268..9441)
                     /gene="ortT"
                     /locus_tag="LGK42_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBK2659924.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
BASE COUNT         2280 a         2367 c         2369 g         2548 t
ORIGIN      
        1 tttcggtaac tgacatacag catcagcgat cgcgctgata ttctgcgcat tatcctgaaa
       61 ccattcatgc cccggccccc atgtgcgcat ggtgcagaga taacagtcca ccagcgttag
      121 ttgctcacca aaggcataag gttcagcggt caattgtgaa ttcagccaga tataaagtga
      181 tttgcgatat tcaattacat tcttctttaa ctgttcaggt gcgtcagatg cccaacgctc
      241 agggtaatcg gcgaaagtga atgttggata gacattagca accagccaga ccaatagccg
      301 ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc gacgatcgag
      361 aaccataaat gcaatcgccg ctgtctccgt cataatctcg tcattttcca gcgccagtgt
      421 cggaacctga cacaacgggt tcagggtttt caataactcg cgtgaagctc cctcgtggtc
      481 aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca tcagctcact
      541 gattgtagag ccccatccgg gtacgccata caccttaatc atatgccctc acaatctttt
      601 aaagattaag tgtaggacag tacacaggtt aaccagcaaa aagatttgtc attttttcgt
      661 cagttgtatt attccccttc aggattgtaa ggggatgttt tgcatcgttt atcgataacg
      721 ggtaggatgc tgcgccgata gttttatacc cataaagaat aatggtgata actatcatcg
      781 ccaggatgaa taaacattgt tcatggcaac ttatatgact ttttcattaa agcaatcagg
      841 gagagcaatg agtaaacacg acaccgacac ttcagatcaa cacgccgcga aacgccgctg
      901 gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc aatcgccagg tacagatgat
      961 cgccattggc ggcgcgattg gcaccggctt gtttttaggt gcaggagccc gactgcaaat
     1021 ggcgggtcca gcattggcac tggtttattt aatttgtggc ttgttttcgt tttttattct
     1081 gcgtgcattg ggtgagctgg tgctacaccg cccttccagt ggcagttttg tctcttatgc
     1141 ccgtgagttt ttgggtgaga aagccgctta tgttgctggc tggatgtact tcatcaactg
     1201 ggcgatgacg gggattgttg atattaccgc cgtcgctctg tatatgcatt actggggtgc
     1261 gtttggcggc gtgccgcagt gggtctttgc gctcgctgca cttaccatcg ttggcaccat
     1321 gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc ttattaaagt
     1381 gctcgccatt gtgacctttt tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct
     1441 ggatggcaac accactggct ttcatttaat taccgataat ggcggcttct tcccccacgg
     1501 tttgctgcct gctctggtgt tgattcaggg cgtagtgttt gcttttgcct ccattgaaat
     1561 ggtgggtaca gctgccggag aatgtaaaga tccgcagacc atggtgccta aagcgattaa
     1621 cagcgtgatt tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc tggttatgct
     1681 attgccgtgg agcgcgtatc aggcggggca aagtccgttc gtgacgtttt tctctaaact
     1741 gggtgtgcca tatatcggca gcattatgaa cattgtggtg ctgaccgctg ccctctccag
     1801 cctgaactca ggtctgtact gcaccggacg tattctgcgc tcaatggcga tgggcggttc
     1861 cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc
     1921 gacactagtt gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt
     1981 tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt
     2041 gtgccagatg cgcctgcgta aagcaattaa acaaggcaaa gcagcggatg tcagttttaa
     2101 actgcctggc gcgcccttca cttcctggct gacattactg tttttactga gtgtcctggt
     2161 gctgatggcg ttcgattacc cgaacgggac ttatactatc gcggcgctgc cgattatcgg
     2221 tattctgctg gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac
     2281 tgcgccagtc gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc
     2341 gagctaattg caaaattgcc cggctacgtg ccgggcaaat ttagattact gacgaaggtg
     2401 tgccgcatgc ggcgtaataa aagtcaggca agtctctccg ttttcaccct cgccacatct
     2461 attacagcgc aatacgaatc acatcgtccg gctgggtagc ttcctgctgt ttagtggatt
     2521 tttgtttcac actgacatac agcgttttgc catcggcaga cagcgccagg ctgtttggat
     2581 gagtcggcgt atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc acactgactt
     2641 tacctgcctg acgatgcgtt acgtaggctt cattacgcgc tgggttaaac agcacagcca
     2701 gtgattctgg tgccgcaacc ttcgccagaa tattgccatt acgggtatcg accactaaca
     2761 cttctgcggc tttagaatcg gtgataaatg cacgctgcct ggcggtatca aggctaatgt
     2821 tgataaagaa gtgctctttg ccgtcatcca gcagcttttt acggctgagg attttattgt
     2881 cggcggtgtc gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt
     2941 tgctatccag cgccagaccg gtactcattt taccggtgtt ctggatggcg gttttcagtt
     3001 tgatattctc gccatcaacg acccaaatca cgctctcttt accaatacca ctgatgtaaa
     3061 cggtgttcga ggcatcatca gctaccagct cacgcggttg cagcgggcgc acctcttccg
     3121 tgcgcttacg atcatccagc accagacggc ctttcacctc gcccgttttg gcatctatcg
     3181 ccgtgaccgc gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg
     3241 tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt cacttccaga gtgaccggat
     3301 caagacgata aaccacgcca cctttatcca gtttgcggct ttgcgaagtg gcgagccaca
     3361 gtgcgttttc ttgctggcta taagccattt cgtaggcacc tttacctacc gctttacgca
     3421 gcatttcttc tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa cctaacagta
     3481 atgaaccacg caggcgcgat gaaaacagat gacgtaaatg catgacgact ccctttgatg
     3541 aaccgatatt tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt
     3601 tataaatggg aatggtaatc attattttct caaatgtgga gcatttcttg ccgttacgac
     3661 tacaattttc tcaattactt aacgtatcgt gccgtttcgt tttccataaa tttacaattt
     3721 cattaccata acggcgtttt cccattctta acctggcttt tttcatgaag attttttccg
     3781 tccgacagac cgttttgccc gcactgcttg tcctttcccc cgttgttttt gccgctgatg
     3841 aacagactat gattgtcagt gccgcaccgc aggtggtttc agaactggat accccagcag
     3901 cagtaagcgt ggtggatggc gaggagatgc gcctggcaac accgcgcatt aacttgtccg
     3961 aatcactgac cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt
     4021 tacagctgtc gattcgcgga tttggctccc gctccactta cggtattcgc ggtattcgcc
     4081 tgtatgtgga cggtattccc gccaccatgc ccgacgggca agggcaaaca tccaacatcg
     4141 atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca
     4201 acgcgtctgg tggggtaatg aatgtcacca cccagaccgg acaacagcca ccaaccattg
     4261 aagccagtag ttactacggc agttttggca gctggcgcta tgggctgaaa gcaacgggcg
     4321 caacgggaga cggcacacag cctggcgatg tcgattacac cgtctcaacc acgcgtttta
     4381 cgacccacgg ctatcgtgac catagtggcg cacagaaaaa tttagctaat gccaaactgg
     4441 gcgtacgcat tgatgaagcc agcaaattaa gtctgatttt caatagtgtg gatatcaaag
     4501 cagatgaccc aggtgggcta accaaagcag aatggaaggc taatccacaa caagcgcctc
     4561 gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg
     4621 agcgtagcct gagcgcgcaa gatgatatga gtgtgatgat gtatgccgga gagcgagaaa
     4681 cgacccagta ccagtcaatc ccgatggccc cccaacttaa cccgtcacat gcgggcggcg
     4741 tgattaccct gcaacgccat taccagggga tagacagccg ctggacacac cgtggtgaac
     4801 tgggcgttcc ggtcacgttc actaccggcc tgaactacga aaacatgagt gaaaaccgca
     4861 agggctacaa taacttccgc ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt
     4921 tgcgtcgcga cgaacgcaat ctgatgtgga acatcgatcc ctatttacag acgcagtggc
     4981 agctgagcga aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt
     5041 ccaacgacca ttacgttact ccgggtaacg gcgatgacag cggtgatgcc agttatcata
     5101 aatggctacc tgccggttcg ttaaaatatg caatgaccga tgcctggaat atctatctgg
     5161 cagccgggcg aggttttgaa acgccgacga ttaatgagct gtcttatcgt gctgatgggc
     5221 aaagcggtat gaactttggt ttaaaaccat ccaccaacga tacaattgag atcggcagta
     5281 aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt tcagaccgac actgatgatg
     5341 aaattgttgt cgatagcagt agcggtgggc gtacgactta caaaaatgcc ggaaagaccc
     5401 gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg
     5461 cgtcctggac ctggcttgat gcgacctatc gcagcaatgt ttgcaatgaa caggattgta
     5521 acggtaatcg gatgccaggg atcgcccgta atatgggctt tgcgtcgata ggttatgtac
     5581 cggaagatgg ttggtatgca ggcacggaag cgcgttatat gggcgatatt atggcagatg
     5641 atgaaaatac ggcaaaagcg ccgtcttata ctctcgtcgg cttattcacc gggtataaat
     5701 acaattacca caatttaact gtggatttat ttggtcgtgt cgataattta ttcgataaag
     5761 aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg atattacgaa ccttcgcccg
     5821 gacgaaatta tggtgtcggc atgaatattg cgtggagatt tgagtaatta ccttgatgcc
     5881 cggtatttgc cgggcattta ctttaacgat tgtattgctg gtataaaata gcaatatttt
     5941 gttgcataat ttcagcaaga caatgtctgc atccctcttt gtcttttgct tttaacgcag
     6001 cgagtaacag atgatagttt tctatatgct cccgtaattc cgctggatta atcgcttcat
     6061 agagataatg taaaccaggc cccatcctga cccacagttg ctcaatcatc tcacacagga
     6121 tgggcatatt tgagcgatga taaatcgcta agcgaaatag cctgtttaca ttaatgattt
     6181 gttccatgtc gcccttttct tgcgcctgct gtaatttctc gagcaattcc tggagttccg
     6241 ccaggtcttg cggggtgagg ttttcaacag ccagagcaac tgccattaat tccagttcgt
     6301 agcggatccg attgatttcg tctaattgac gtttcctcac ttccggaact gtaaatgctt
     6361 gtgcaggtgc gacagaaagc gcattcaccg aaaccagacg taataatgct tcacgcacag
     6421 gtgtaatact catacctaat tgctccgcca gatttttagt aatcaggcgt gcgccaggtt
     6481 ttaatgcgcc aatactaagc tgatgtttca ggtcgttctc aacctgcaga gtcaaactga
     6541 catgtttcat ttttcccatt ccaggcattt taattccttc gcgtgttttc tgtccaaccc
     6601 tgcctgcacg gcagaaggag ttcacctggt gtattaaagt gaataatcat aaaccaccca
     6661 cctttgttgt tattagcagc agaacaatga cgtggttagt taattaaagc tgaaaataag
     6721 ctttttgcct ggatttttat ggcggcttac gccgccgttg aaactttaat catcacccgc
     6781 gacgcggatc accactttgc cgaagttttt acccttcagc aggccgataa acgtctgtgg
     6841 cgcattctct aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca
     6901 ttgccccatc tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa
     6961 cccttgcaag cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg
     7021 accgggtggt agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc
     7081 agatgtatta agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata
     7141 gatatcaata ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg
     7201 atcaagacaa acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc
     7261 ggcaaccccc accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac
     7321 aggacctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag
     7381 cagccccata taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt
     7441 ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca
     7501 tccactgtag cccagcaccc agtcgccagg ctgataatca ggatgattcg actccacaac
     7561 acggctcacc gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg
     7621 ctcatcgctc atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa
     7681 cacctgtcct tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac
     7741 cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc
     7801 cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggatta
     7861 gttttgtttg cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg
     7921 ttgcttcggg ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca
     7981 gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac
     8041 cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc
     8101 attgcctggt ttgaagcgcg gaccatagca ggttatccgg tgctggtgag tgaagaagat
     8161 ggcgtagtga cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat
     8221 accgtggaac attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa
     8281 ttgttaagcc gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg
     8341 atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc
     8401 gcacaaatgc cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag
     8461 ttgcaactcg acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg
     8521 cttttttgat tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca
     8581 cccagacctc ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc
     8641 tctttgtttc tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca
     8701 gcgtgcgctg gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta
     8761 tcagcggata tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc
     8821 taattggcgg tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt
     8881 tcggcccaat ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct
     8941 cattttaatc agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct
     9001 tctttatgct tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag
     9061 cgatcttccc gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg
     9121 gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac
     9181 atactttctc cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg
     9241 tggtgccgtc aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa
     9301 actcatcggc catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc
     9361 gtgagaaaga aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag
     9421 catatgttga tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat
     9481 atgcgtcatt ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac
     9541 agcaacttgc ccttatccat cttc
//
LOCUS       DAFAVF010000159         9357 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFAVF010000159 DAFAVF010000000
VERSION     DAFAVF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.161"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1256
                     /locus_tag="LGK42_003730"
     CDS             <1..1256
                     /locus_tag="LGK42_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2659925.1"
                     /translation="LYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQI
                     LPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSL
                     ALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHT
                     ATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNY
                     TIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLL
                     SRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEV
                     FGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISL
                     VMTEADKALREAKSEGGNKVIIHHI"
     gene            1437..2927
                     /locus_tag="LGK42_003731"
     CDS             1437..2927
                     /locus_tag="LGK42_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2659926.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2970..3473
                     /gene="yeaK"
                     /locus_tag="LGK42_003732"
     CDS             2970..3473
                     /gene="yeaK"
                     /locus_tag="LGK42_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBK2659927.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3474..3578)
                     /locus_tag="LGK42_003733"
     CDS             complement(3474..3578)
                     /locus_tag="LGK42_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659928.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3748..4194
                     /locus_tag="LGK42_003734"
     CDS             3748..4194
                     /locus_tag="LGK42_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK2659929.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4151..4972)
                     /gene="nimR"
                     /locus_tag="LGK42_003735"
     CDS             complement(4151..4972)
                     /gene="nimR"
                     /locus_tag="LGK42_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBK2659930.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5069..6250
                     /gene="nimT"
                     /locus_tag="LGK42_003736"
     CDS             5069..6250
                     /gene="nimT"
                     /locus_tag="LGK42_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBK2659931.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGGDKEI
                     R"
     gene            6305..6652
                     /locus_tag="LGK42_003737"
     CDS             6305..6652
                     /locus_tag="LGK42_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBK2659932.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6674..6928)
                     /gene="yoaF"
                     /locus_tag="LGK42_003738"
     CDS             complement(6674..6928)
                     /gene="yoaF"
                     /locus_tag="LGK42_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBK2659933.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7111..8136
                     /gene="dgcP"
                     /locus_tag="LGK42_003739"
     CDS             7111..8136
                     /gene="dgcP"
                     /locus_tag="LGK42_003739"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBK2659934.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8169..8267
                     /locus_tag="LGK42_003740"
     CDS             8169..8267
                     /locus_tag="LGK42_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBK2659935.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8270..8344
                     /gene="yoaJ"
                     /locus_tag="LGK42_003741"
     CDS             8270..8344
                     /gene="yoaJ"
                     /locus_tag="LGK42_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBK2659936.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8403..8651)
                     /locus_tag="LGK42_003742"
     CDS             complement(8403..8651)
                     /locus_tag="LGK42_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK2659937.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8879..>9357)
                     /locus_tag="LGK42_003743"
     CDS             complement(8879..>9357)
                     /locus_tag="LGK42_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2659938.1"
                     /translation="IRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRN
                     VRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRYACCGHTAKENRL
                     SQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2307 a         2259 c         2194 g         2597 t
ORIGIN      
        1 cattatataa cgaaaacaca aatgtaaaac tcatcccaca gatgaattac ctgatggttg
       61 ttgtggcttt gtttttcctt aacgccgtca tttttctttt catgttaatg aaatatttca
      121 ctaacaaaca aattttacca acactcattt taagccttgc atttttaagt ggccttatct
      181 atttagttga aaccattgta attatccata aaccaattaa cggcagtaca ctgatccaga
      241 caaagtcgaa tgatgtttct attttctata ttttccgcca actcagtttt atttgtttaa
      301 cctcgctggc actcttttgt tatggaaaag acaacatcct tgacaacaat aagaaaaaaa
      361 cgggaatcct gttgctggcg ctgattcctt ttttagtttt tccccttctg gcacacaatc
      421 tgagcagtta taacgctgac tattctttgt atgtcgtcga ctactgtccg gacaaccata
      481 ctgcgacctg gggaatcaac tatacaaaaa tattggtttg tctgtgggca tttttactgt
      541 tctttattat catgcgtaca cgattagcca gcgaactatg gccgttaata gcattattat
      601 gtctggcatc gctatgctgc aacttacttc tactgactct ggatgagtat aattacacca
      661 tctggtatat cagtcgcggg attgaagttt ccagtaaact gtttgttgtg tcttttctga
      721 tttataacat ttttcaggaa ctgcaactct ccagcaaact ggcagttcat gatgtgctga
      781 ccaatattta taatcggcgc tactttttca acagcgtaga gtcattattg tcgcgacctg
      841 ttgttaagga cttctgtgtc atgctggttg atattaatca gttcaaacgc atcaatgccc
      901 aatggggaca tcgtgtgggt gataaagtgc tggtttcaat tgtcgatatt atccagcaaa
      961 gcatccgccc tgatgatatt ttagcgcgac tggagggtga ggtgtttggc ttgctattta
     1021 ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac
     1081 tcctgaccgg ctttagtaac agatatgatg ttcctgaaca aatgaccatc agtattggca
     1141 cggttttttc aacgggtgac acgcgtaata tctcgcttgt catgacggaa gcagataaag
     1201 ccttacgcga agcgaaaagc gaggggggca acaaagtgat tattcatcat atttaagtgc
     1261 aaaaatattc agagccatgc tttttgcgtg gcttttgcat acaatttatt actattaccc
     1321 ctaaatttcc cctccccagc ggcgcagaga tgagtataat tagcgcccct gtgccaggcc
     1381 gcaatcgaac tttatctggt tttctcgttt cactaaccga aggagtgcca tttatcatga
     1441 aattgcacca tagaatgctc cggcatttta tcgccgcaag tgtcattgtg ctgacatctt
     1501 ccttccttat ttttgaactt gtcgccagcg acagagcaat gagtgcctat ctgcgctata
     1561 tcgtgcagaa agcagactcc tcctttcttt atgataagta tcagaatcag agtattgccg
     1621 cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg
     1681 ccatctgcga ggcttttgag tctgccaata atacccatgg cttaaacctg actgcccata
     1741 aatacccggg cttacgcggc acactacaaa ccgcatccac tgactgcgac acaattgtgg
     1801 aagctgcagc actattaccc gcttttgatc aggcagtgga aggcaaccgc caccaggatg
     1861 attacggttc aggtcttggg atggccgaag agaaatttca ctattatctc gatctcaatg
     1921 accgctatgt ctatttttat gagccggtta atgttgaata ctttgcgatg aataactggt
     1981 ccttcctgca gtcaggaagt attggcatcg atcgcaaaga tattgaaaag gtatttaccg
     2041 ggcgtaccgt attgtcgagc atttaccagg atcagcgtac taaacagaac gtgatgagtt
     2101 tgctgacgcc ggtatatgtc gcagggcagc taaaagggat tgtgctgctg gatattaaca
     2161 aaaacaattt gcggaatatc ttttacactc atgaccgccc tctcctctgg cgttttctca
     2221 atgtcacgct aaccgatacc gattcggggc gcgacattat catcaaccag agcgaagata
     2281 atctgttcca gtatgtcagt tacgtccatg acttaccggg cggcattcgt gtctcgttat
     2341 ccattgatat tctttacttt atcacgtctt cgtggaaaag cgttctgttc tggattttga
     2401 cggcgttaat tttgctgaat atggtgcgga tgcacttccg cttataccaa aatgtgtcgc
     2461 gagaaaatat tagtgatgcg atgactggac tgtataatcg caaaatttta acccctgaac
     2521 tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc ggtgatgttt attgcaattg
     2581 acatggacaa gttaaagcta ataaatgaca ccctcggtca tcaggaaggg gatttagcga
     2641 ttacgttgtt agctcaggcg attaaacaat cgattcgtaa aagtgattat gccatccgac
     2701 tcggtggcga tgaattctgc atcattcttg tcgattcgac gccgcaaatt gcagcacaac
     2761 tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc gcagaaagag atcggcttct
     2821 cttccggtat ttacgcgatg aaagaaaacg atacgttaca tgatgcgtat aaagcttccg
     2881 atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa ccttctgtgg
     2941 ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga agcgtgacgc
     3001 atcagcggtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc gttacccatg
     3061 aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg caccgcttta ggccagggag
     3121 ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt ctggcgatcc
     3181 tcgctgccga tcaacaggcc gatctgagcc aactcgccag ccatataggc ggattacgcg
     3241 cttcgttggc cagcccggcg gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc
     3301 cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc ttcgaacgat
     3361 ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg aaaaccacgg
     3421 attatttgcg cattgcgcaa ccagaactgc tcaatttccg ccgcactgcg taactagcct
     3481 gtccgttcaa taagcagaac ggacaaaacc agcacaacag caatggcaaa aaacgatgac
     3541 gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg ggagatagct
     3601 gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg cctgattttt
     3661 tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcgtaaa gtaagcaggc
     3721 ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat cctgctcggg
     3781 ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat tctggtgttg
     3841 atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa acaggggcta
     3901 agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag cgggacgcta
     3961 ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc aattgcagta
     4021 ggcgttattg tctcctggct gggtgggcgc ggcgtgacct taatgggcag ccagccgcaa
     4081 ctggtcgccg ggctgctggt aggcactgtt ttaggcgtgg cgctgtttcg cggcgtaccg
     4141 gtcggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca gtagttaatc
     4201 ccgcaatata tcggcctggc gtttgcccca ggcctttctt aaacatagta ataaaggccg
     4261 ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct ttcactaatc
     4321 cctgaagtgc cataatcagc tgtaactgct gacgccactg gcgaaaactc aaccccgtct
     4381 cttttactat cagtcgggca agattgcgtt cactcatcgc aaaaaagcca gcccattgcc
     4441 ctaatgaccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg cggattttag
     4501 gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca
     4561 ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca
     4621 gggttaaaat caattcccgg cataagggcg atattttcaa tgtgcaacat gttgtcggca
     4681 ttgtcactgc gctgggttcg ataaacagaa aacagagttc agcgttagct gtcacttgat
     4741 tgctatgttc caccccgccc ggtatccaaa ccgcatattg cggcggcacc atccacaaag
     4801 cattttccac cgtacaggta attgcaccat gtaatgctag tatcagctgt cctttgcggt
     4861 gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg
     4921 cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg
     4981 aatttagcga taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta
     5041 cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa
     5101 caggattgtc cttatcgctg gcattctgat gattgccaca acattacgcg tcacctttac
     5161 cggcgcagcg ccgttactgg atacgattcg ttccgcttac tcgctgacga cagcgcaaac
     5221 cggcttatta accaccctgc cattattggc ctttgcgcta atctcacctt tggctgcccc
     5281 ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc
     5341 aggtatcgca attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat
     5401 tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc
     5461 tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact
     5521 gggatcggct atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt
     5581 catgctgatg tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagcca
     5641 acaacatgca aatttgagta cctcgcgcgc cttacatact cggggtatct ggcgttcgcc
     5701 gcttgcctgg caggtcacat tgtttcttgg gatcaactca ctggtctatt acgtgattat
     5761 tggctggctt ccggcgatcc tcatcagtcg cggctatagc gaagcacagg cgggttcact
     5821 gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatccctc ttttcttaca
     5881 tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc
     5941 tgttgggctc tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcggttttgg
     6001 ttccggcgca acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca
     6061 tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg
     6121 gccgccgctg atgggtaaaa tacacgatgc taacggtgac tggtccgtac cactgatggg
     6181 tgttgccata ctttcactac tgatggcgat tttcggactt tgcgccgggg gagacaaaga
     6241 aattcgctaa tatccggtgc tatagtgacg tgacaaatca tgcgtgaaag ggagaacaaa
     6301 cacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg
     6361 cgtactggtc gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga
     6421 gtgggataaa gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt
     6481 cgattttgca acctttcgcg agcaatatct tgcagaactg gcgcaacacg agcaagaagg
     6541 aaagcggctg gcggacatcg ccaaaaaaca gccgctgacc ctgctctacg cagcaaaaaa
     6601 caccacgcag aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta
     6661 cagcatccgg cggttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat
     6721 caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg
     6781 gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct
     6841 gcggcggttt aggtgcttct ggctgagaag aggttgaaca accagccagg accagcaagc
     6901 aaggcaaagc aaaactgata attttcattt attgatctca cacatttatc tacgcttaga
     6961 gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc aaaacatatt
     7021 acgtcttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag
     7081 catttacccg ccgtatcctg gagttgtttc gtgtcagatc agattatcgc ccgcgtctcg
     7141 caatcccttg ccaaagaaca gtcactagaa agtctggtcc gacagcttct ggagatgctg
     7201 gaaatggtca ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg
     7261 cagcatatta tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc
     7321 tcgtgggatt actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa
     7381 gtccctgacc gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg
     7441 agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt
     7501 gagaagcgcc agtggagtga acgcgcggaa caggttttac agttattcgc tggactgatt
     7561 gcacaatata ttcaaaaaga ggcgctggtt gaacagctgc gcgaagccaa tgctgcgctg
     7621 attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc gatttttgaa
     7681 aatctgacga cgctgttttc tctcgcccgg catcttaacc ataagataat gatcgcgttt
     7741 atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag tggcgatctg
     7801 tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga agttattggt
     7861 cgtctcggcg gtgatgagtt tttggttgtt tcactaaaca acgagaatgc ggatatttcg
     7921 tcgctgcgag aacgtattca acagcaaata cgtggagaat atcacttagg tgatgttgat
     7981 ttgtattatc ccggtgccag tcttggcata gtagaagtcg atcctgaaac aaccgatgca
     8041 gacagtgccc tgcatgctgc cgatattgcg atgtatcagg agaaaaaaca caaacagaaa
     8101 acaccttttg tcgcgcatcc agcgctacat tcctgaggcg tattcacatc cttttgattg
     8161 gtgataacat gcgaatcggt attatttttc cggttgtaat ctttattaca gcggtcgtat
     8221 ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga tgaaaaaaac
     8281 aacgattatt atgatgggtg tggcgattat tgtcgttctc ggcactgagc tgggatggtg
     8341 gtaacgtcac ctctaaaaaa tagcaaaggc tgcctgtgtg cagcctttgt gcaatttaag
     8401 cgttaacttt taatcttcct gtagataaat agcacgacaa tcgcaccaat aacggcaacc
     8461 acgaagctgc caaaattgaa gccatcgact ttaccaaagc caaacagtgt gctgatccat
     8521 ccgccgacta cggcaccgac tatccccagc agaatagtca taaagaatcc accaccatct
     8581 ttacctggca tgatccactt cgccagaata ccggcaataa gcccaaaaat aatccatgac
     8641 agaatgccca ttgtttcctc acttatctgt tttgcattag cgggttagtc gctgataaaa
     8701 agcatagcac aacatcggga gggcaagatt tgtgacgagc atcacggagg atttttttgc
     8761 ggatggcaca gaaattgcgc catcaacgat cagtgataat taccaaccac aaacatcatg
     8821 ttcgttttcc gtgtcttgac atcctccacg ccctgaatga cgaggatccc tgctacgttc
     8881 aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa cgggcggcct gactgcacca
     8941 tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat gttacgagcg ccgtttacat
     9001 cggcgttcgc tgtatatcca catacctggc atctgaattg actttgtgac aggcgatttt
     9061 ctttcgctgt atgaccacag cacgcgtaac gctggcttgt gtacgctggc ggaacagcaa
     9121 gcacctgacc gccacgccag agctgcttgt actcaagctg gcggcgcatt tcataccagc
     9181 cctgatccag tatcgaacgg tttaaacctg attttgcccg gacattgcgc cccggctggc
     9241 tgaccgtacc cgctgctgac tttgacatgt tgctgacctt caaatcctca atgactatca
     9301 ttgcgtggtt tttgctgacg gtcgttgtga ctttgtgaag gtagtccctg cggatat
//
LOCUS       DAFAVF010000160         9332 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFAVF010000160 DAFAVF010000000
VERSION     DAFAVF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.162"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            529..1596
                     /gene="pilL"
                     /locus_tag="LGK42_003744"
     CDS             529..1596
                     /gene="pilL"
                     /locus_tag="LGK42_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBK2659939.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWNNKTLTVTTSAAAPAQDTATTISPAAMVTTSTYPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1596..2033
                     /gene="pilM"
                     /locus_tag="LGK42_003745"
     CDS             1596..2033
                     /gene="pilM"
                     /locus_tag="LGK42_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBK2659940.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            2047..3729
                     /locus_tag="LGK42_003746"
     CDS             2047..3729
                     /locus_tag="LGK42_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBK2659941.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3722..5017
                     /gene="pilO2"
                     /locus_tag="LGK42_003747"
     CDS             3722..5017
                     /gene="pilO2"
                     /locus_tag="LGK42_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBK2659942.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            5004..5456
                     /gene="pilP"
                     /locus_tag="LGK42_003748"
     CDS             5004..5456
                     /gene="pilP"
                     /locus_tag="LGK42_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBK2659943.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPIIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5467..7020
                     /gene="tadA"
                     /locus_tag="LGK42_003749"
     CDS             5467..7020
                     /gene="tadA"
                     /locus_tag="LGK42_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK2659944.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            7033..8130
                     /locus_tag="LGK42_003750"
     CDS             7033..8130
                     /locus_tag="LGK42_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBK2659945.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            8148..8762
                     /locus_tag="LGK42_003751"
     CDS             8148..8762
                     /locus_tag="LGK42_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBK2659946.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            8772..9332
                     /locus_tag="LGK42_003752"
     CDS             8772..9332
                     /locus_tag="LGK42_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBK2659947.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
BASE COUNT         2713 a         2243 c         2105 g         2271 t
ORIGIN      
        1 gacaacttat cattgcctta tatgttatat ttctgtgtgg tggttgtgat ttgcattttc
       61 ccactacttt tcagatgtgt acttcttatt gtaaggtctg catctaaata cgacttcaca
      121 ttctttgtga agaagaaaaa gggaagcaag aaatcaacta tacatcttag cccctatgct
      181 gacacacgtt gcccgaagtc aatgaggaaa cactgggatg tattgctttc tctgatatca
      241 aaagcaatta ataatgatga aatcattgtc atgcattcac atctctttac tccagcaaga
      301 ataaataaac ttataaagag actggaatct tatgatttga gtttttcagt tattacatac
      361 ccacggaaaa caagtctgta cgaaaaatta tcaattcctt ttatatatct cttatttcag
      421 tggtctttcc cgcatttgca ttcggacgga atgacagtcg tcattcgtcc tctccagccg
      481 cctcacacca attaacaaac acgtatatac gaacacggag tctccgttat gaaaaaaaac
      541 tgtctgacac ttatactgcc gtcattattg tccggctgtg ccggaatttc cggcagcaat
      601 aacgtggcac catcagatac gcctgctctg gtttttgttg acggacaaat cagtgaaagt
      661 gctggcgttc tggcacttac gcaacaacgt ctttctccac ctaaaataac tccccccccg
      721 agagtcagcg tgccagagac agtaaaaaca gcacagcctg ttagcgaatc ccgaccgtta
      781 ccttcaccaa caataagtca gaatttctcc cggttaatca ttacaggcaa gcctggtagc
      841 gcccctgtaa cactatcgcc gacggcccgt aaccggaccg tcagtcagtg gataaaaaca
      901 ttactaccgg aaggatggaa attcagacaa gagaatgcag ccacgcccaa gctgaatacc
      961 aaactggttt catggtctgc caatgatcag tggacccgtt ctctgaaccg gttgctggaa
     1021 gaacaacacc tctggggcca tctggactgg aataataaaa cgttgaccgt cacaacatca
     1081 gctgctgcac cggcacagga cacggcaacc actatctcgc cagcagccat ggtaaccaca
     1141 tccacttacc ctgaatctcc aacgacagca aatagccaaa acaaaccacg aaatccattc
     1201 aggggaaaca gcgtatcttc gtcaacgccc gccgccaccc cgactccaac cggctccaca
     1261 gtcaagtcta tcccgctcat gaccggaacg cctgttaaac cggtatctca gggtaaagag
     1321 tggcgtgcgc cggcaggcac cacattaaga gagaacatta tcaagtgggc agaagaaaca
     1381 aaatgcgaaa gcatggcatc aacgcactgg atggtcatat ggccactttc tgtaaccgat
     1441 taccgtctgg atgcgccttt aatgttccgg ggatcatttg aatcagccat ggagcaggta
     1501 tttgagttat atcggactgc gcaaaaacct ttatacgcac aggcttctcg catgcagtgc
     1561 atcattacag tttctgatac aagggatggt cgctaatggg atggctggtc atggccgtcg
     1621 gcctgaccat tctgattatc actggtagct accagaatca gaagatgtct gaaaccacaa
     1681 acgcccagca atatgcaagt gcgtctgtat gggcaagtca gatactaatg attgctaaca
     1741 gaatcaatga tatcagatac gtatcaggcc agcaggacgg agtaatttcc tcagataaac
     1801 tggctctccc tgtcactccg gactcccgaa ttaaacatca gttacaacag gggcgtctgt
     1861 gggtatggat gcctgaacaa ccaggtcttg ttgaaacttt gcgttcaaaa agtcgtggtt
     1921 ctgcactaat agggatcttc caaaacgggc aactcacctg gctatcagga actgcaaccg
     1981 gtctgacacc cccggcaggc attactgctg gttctgtcgt ctacgttaac taatctggat
     2041 atattcatga aaaaatcaca ccagcgttca atgaagctgg cggtgctccc ctgcatgatc
     2101 gccgtcgctc tttccatctc cggatgtact ttcagcgaaa tcaacaaaat gcagaaaaaa
     2161 gcacaggaag actcagcaca tgcacgggaa aaggtatcag ccctttcggc ccgtaaatcg
     2221 caggctctta cctggctcga taatcaatgg ataaaccctg ttccggtcgc tcaggtatca
     2281 agagagaaaa aacaaacagc tccggcctgc tacatcacgc aggcaagaaa aggagagatc
     2341 actctgcagg aactggggca acgtattact gccgtatgcg gcatccctgt gatcatcacg
     2401 cctgacgcag ccaattcaac tcttgaagga ggcgctaccc gccagatgac aggaacacta
     2461 ccagcaccag atgaaaatgg gcgtctaccg ttaagcagcc tgggcagcac aacaatgact
     2521 acctccactc agccattaac gctgaataac ctcatgtggc agggagatat caatggtctt
     2581 ctggatctga tggccagccg gagtggtctg tactggcgca tggataatgg tcggattgta
     2641 ttctatctga ctgaaaccag aacgtatcca cttcatatgc tgaacaccaa aaccagcagc
     2701 agttccagtg tcagctctgg ctcaacaagc acaatggggg caacaggagg ccaggataac
     2761 tcagcatccg gtgatgcaac gtcctctcag agcacaaccg ttggtcagga atacgatctg
     2821 tatgaagaca tccggaaaac tattgaagca atgctgacac cagaaaaagg ccgttactgg
     2881 ttatctgcat cgagctcaac gctgactgtc actgatactc cagctgtcca ggaagccgtc
     2941 gcacgatatg tggacgaaca aaacagtatt atgaaccgcc aggtagccct gaacgtacag
     3001 gttctgagcg tcagcaatac cagaaacgaa cagttcggtc tggactggaa ccttgtttat
     3061 aaatcgctac attccgccgg agcaacgttg aacaatgcaa gcggagattt tacaggcgct
     3121 acatctgcag gcgtatcaat tctggatacg gcaacaggga atgccgccaa attcagcggt
     3181 tccagtcttc tgattaaagc gctgagtgaa cagggcgatg tcagtgttgt gacttcacaa
     3241 gaaagcactg tcacaaacct gacgccggta cctatccaga tggcagatca gacggtttac
     3301 gtcgcccagt cagcaacaac aacgactacg gatgtaggag caacaacaac attaacgccg
     3361 ggcatgatca ccaccggatt caatatgacc ctgctgcctt taattcagaa aacgggcaat
     3421 ctccagttgc agatgaattt taatctgtca gatcccccaa caatccgtag ctttacgtca
     3481 aaagacggaa acagttacat cgaaatgccg tataccaaac tgcgttcact gagccagaag
     3541 gtcaatctga aagaagggca atcacttgtc gttactggtt tcgatcagaa caatacgacg
     3601 acaagtaaag ccggtacgtt tacgccagca aatccattat ttggtggttc acaaaccggg
     3661 aaaaatgaac gcagcacgct tgtaatcatc attaccccga ctttcccgtc aggaggcaac
     3721 aatggctgat gaagatatca acccggttgt gcttctcgca gatcctaaag taaatcatcg
     3781 cgtgtgggca gcctgtctta aatggagtcc tgtcgtaaaa aaacagcgag tcccttctca
     3841 tcagaaacac aagccccatg taaaatccag acggcttacc tctctgaaag tgactgtagg
     3901 cagcagaagt tcccgtggga agatcagccg cattaccggc acaggaatac tggccaggcc
     3961 ggagcgcaat cattattttt cccttgctct ggctttctgc tcatgggtga gaaatggata
     4021 cggcgtcttc cggtacagcg ataaggagct tttattcctt gcctccatta atggccagcc
     4081 tgccgttatg gctgatctgt caggaaatga tgctgatgtt gctcaaaagg tcagtctttt
     4141 tctgacgatg aacgaagagc cccccgaaaa atggcaggtc gtatctcctc tggaacatcc
     4201 tgataactgg gaaagtatta ttacccggtt atcatcagca gatctccgcc gttgcaaact
     4261 tactgtcggc aatcgcagca aattcacact gcctgccgta ctgtttttag tagcagccgg
     4321 cgcaggcact gtattctgga tgactcagcc agagccggat gtcgtaccga cgcccgagga
     4381 gatagctgca cgggcaagat tgcagtttaa aaagcccgat ccaccgccag aactcccgca
     4441 tccatgggca tcacaacctg taatcagtga ttttctgaaa gcctgcgcgg accttcgaaa
     4501 accatcaccg gtcgcgctag aaggctggaa acttaccggt ggaacatgta ctcccgagac
     4561 attcacactg atttacgaac gacagccagg aggaactatt gaaggctttc tggcaagaag
     4621 caaagaaatc tttaatgtta ttcctgactt taaccttaaa gatggtgcac gcctggcttc
     4681 tgtaacccgg ccactgcctt ccctgccccg cagggatgag gcagtcccta ccccctcaga
     4741 acaactgatg cgggtgttca cgtggtttca gaaaaaacag ttaacgccgg ccattaatga
     4801 aatagccata ccggaaccat taccaggtaa cgatggtgaa ccagcccctg ttcagaaatg
     4861 gaaagaatat cagttcagtt tatcaacgcc tgtaaatcct gatgagttgt tccctttatt
     4921 tcaggatacc ggcgttcgtc tgagcaatat ccattttgaa ttaaacggcg gtactttcag
     4981 ttattcctca gaaggtcata tttatgcgtc caggtaaatt actcattatt ccatccatat
     5041 ttttcttctc cggtttttca tttgcaacaa cacaacctct tgtcacaata ggagaacttg
     5101 aagcacaaca aaaccggaat attcttttac aggcaaaagt tcagggcgca cagctacaga
     5161 aacaactcga agaatcagat gttacttcat catctgaaac tgtttcaggt ttctctggtg
     5221 tgactgcctc tttgccatca gtttcagagc agccgacatc taaaaacaga aaggaattgc
     5281 ccatcattat ggaaataaac ggcaaagata aacgtcttaa tgccgttctt cgcatggctg
     5341 atggacgtca gacaagcgtc accactggca gccagcttcc aggcacatcc gtcacagtga
     5401 agtcgatctc attgtctggc gtcacgttaa gtgatggcac cacccttact ttctgagtaa
     5461 cgtgatatgg ataaatataa tctgaaggat gctctccttt ttatatccag gggagacacc
     5521 catgaaattc ttatagaaac aaaccagcgg acaaggcctg acgtacaaag taatctccag
     5581 gagctgctaa aattatatcc ggatatcaac ccaaaagtag tctcgttgtc ggagttacag
     5641 gaggcaggtc aggatgagaa aaacagaggc ccgaaagaac gaattattca tctgaaagac
     5701 cttgctgatg tcagtgaaag tgagaagaaa gttctatctt attttgaaac tgccagaaaa
     5761 cttggtgctt cagatattca tttcttaatt tcagagtcta ttttcaaagt tcgcatgaga
     5821 atattcggtg aactgcagac tgtagatgaa gatcaaccag cactgggcta ttcactttgc
     5881 gcaacagcca ttctgtcaat ggctgatgtc actgaaacca gtttcttccc tcaaagggag
     5941 caggatgcgc gcctgtcccc acaattaatg cgaaaaatag gaatatttgg tgcacgctac
     6001 agtcacagac ctacgggaga cggtctgatt gcagttatgc gcctgatacc cgatgacgga
     6061 gataaagttc ccacgtttaa acaactgggc tttatcccgg aacaaatccg gttgctgaac
     6121 ataatgctgc gccgcccgga aggaaaaata gtcctgtcag gtccaaccgg ttccggtaaa
     6181 tcgacaacgt tacgcagcgc ctgccgtgta tatcttgacg ataatcaggg gcgacacctg
     6241 ctaacaatcg aagatccgct ggaaggacag atactgggag caacacagac tccgattatc
     6301 tgcgacaaat ccgacgaaga tgccgtcaaa ctggcatgga gtcgggctat ttcatcggca
     6361 atgcgacttg accctgatgc catcatggaa ggggaaatgc gcgatcttat ctcgatgatg
     6421 tcaaccacct acgcggcaca gaccgggcac atagttctga cgacattaca tacaaactca
     6481 gctctgggta taccggagcg aatgattacg atgggtatga atgccgatct tatttgcgat
     6541 gcgcaattac tgattgggat gatcagccag cgccttgtac caactctgtg tccctcctgc
     6601 cgtattccat gggaaaagcg agcacctgaa cttagcgacg acgagcgtga ttaccttgag
     6661 cggcactgca ataaagattc actctgcagt acagataata tctggttccg aaatcatcac
     6721 ggatgctctg aatgtaatca cgacgtaatc attaacggca gaaagcgcgg tgaaataggt
     6781 aaaggcctca ccggcaggac tgtaattgct gaggtgatag aacctgataa ccgcttattt
     6841 cagattctga aaacacgcgg caaagttgca gcacgtaaat actggctgga aaacatgaaa
     6901 ggcatcagtc gtgtagagca tcttcttcgc cgtatcaatg aaggtctggt agaccctctt
     6961 gaagccgaca gaatcatccc tctggacgaa gacgagcgcc tcagcatcga tgatgtctaa
     7021 caggagaaca acatgcgtga aatgaatttc agtcaacggc tgaggcgttt cattgtcaga
     7081 aaaacgttca gcgcccccta cagggtgcag ttttacgaag ccctgcgttt tctgctggaa
     7141 aataaacagc cattaaagac cgcactggaa cagatgcggg atgcctggac agactttgga
     7201 cgaaaatggc atccctttgc tgaactggcc acagactgca ttgagtctct tcgtgaaaac
     7261 agtggcgaaa actctctgga atatactctt agtttatggg ttcctcagga agaggccgca
     7321 gtaatcagcg ccgggataag aagtggttca attgtggatg cactgcaatt cgccaccact
     7381 cttacagatg caaaagaaca aatccatcag gctatatggc aaatggctat ttatccggtg
     7441 ggattgttaa tcatgatgac ggggacatta tatgttctga atacagagct aatacctgaa
     7501 ctgagcaaaa taagctcccc cgattcatgg agtggtgctc ttggttttct ttatggatta
     7561 tctgtttttg tcgataatta cggcgctata tgtgccgttc tttttgcagt gatcacaggt
     7621 ttaatatcat ggtctctgcc aaactggaaa agccctgaca gtgttcgtac ttttgccgac
     7681 aagataatgc catggagtat ttatcaggat atacagggag caacatttct cctgaatatg
     7741 gctgcacttc tgaaagccaa aatgacgacg ctcaactcgc taaacattct tcaggagttt
     7801 gcatcaccat ggttaagcac acgtcttgac agcattattt accgcgtccg ccagggggat
     7861 catcttggac tggctttgcg tcagtgcggt tatcagttcc catccaggga agcagccaac
     7921 ttcctgtctt tattacaggg agatggcgca acagagctaa tcagcaatta tggtcaacga
     7981 tggcttagtc aaacacttca acgtgtaaag aaacgggcaa acgtgattcg tttaattatg
     8041 ctgatatttc ttgtcatgtc tctcatgtta ctggtttttg ccattatgga tattcagtct
     8101 attagtgaca acagcatggg taacttttaa acaaaacgag gatatttatg ttagtagaaa
     8161 atattaatac cactttaact ggaaataata agaagaatga gcctcatgat aaaggatggg
     8221 caattttgga gcagggaaca atagcgctcg ttgttctttt tgttatagtc gttgttctgg
     8281 gtagtttgta tgcgttgcgt acaagagcca atgtggcaac tgaaaccgca aatatacaaa
     8341 caattatcac aagtgcccag agcctgctaa aaggtagcga tggatataca tttaccagca
     8401 gtgccaaaat gacaggtgcc ctcattcaga tgggtgtcat tccctctggg atgaccgtac
     8461 agggcgacaa aacatctggt acagcaacgc tctataacgc atggggcggt gctgtaactg
     8521 ttgccccggc ttcaacatca gggttcaaca acggttttac cgttacctat gataaagtcc
     8581 cacaggatgc ctgtatccag attgccacac gaatcagtaa aactggttta actaacggca
     8641 ttaccctgaa cagtactgct cacagtgatg gaaaagtcac tactgaagaa gccagtacgc
     8701 aatgcaaggc agataatggc agcactggta caaacaaact tatcttcacc atcaatggtt
     8761 aaggagtttt aatgtcaggg ggtacagtac ccctcagact tatttcatta atcctgcttt
     8821 tttctcttat tccacccgcc cgagcttcat acgtagctcc caaacagatc tggaccaaca
     8881 agtgggacaa ttgctttgcc gctgccggcg cgcgctatca aattgaacca ttattattga
     8941 aggctatttc tgccggggaa tcttcgttga ggccgggcgc aattaatata aataaggaca
     9001 aaaaaacagg taaagccagt agcacggatt atgggttgat gcaaataaac tcaacccata
     9061 ttccaaagct gataaatatg ggcgtaataa aaaagtctga ggatttaatt accaaaccat
     9121 gccttaatat ccatatagga agctggatac tggcaagaca ttttcagata tgtggcgtca
     9181 gctggaattg ccttgggtca tacaatgccg gattccgtaa ggacagacat gaaacaagag
     9241 agcaatatgc caataaaata tggagaattt atcgtgacat gaaagggata tgtcttcccg
     9301 gacagggagg caggcaatgc agacaatcgt ag
//
LOCUS       DAFAVF010000161         9281 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFAVF010000161 DAFAVF010000000
VERSION     DAFAVF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.163"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..1025)
                     /locus_tag="LGK42_003753"
     CDS             complement(<1..1025)
                     /locus_tag="LGK42_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2659948.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDT"
     gene            complement(1025..2797)
                     /locus_tag="LGK42_003754"
     CDS             complement(1025..2797)
                     /locus_tag="LGK42_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBK2659949.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            2971..3825
                     /locus_tag="LGK42_003755"
     CDS             2971..3825
                     /locus_tag="LGK42_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBK2659950.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFSRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDSTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            3884..4957
                     /locus_tag="LGK42_003756"
     CDS             3884..4957
                     /locus_tag="LGK42_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBK2659951.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            4961..5704
                     /locus_tag="LGK42_003757"
     CDS             4961..5704
                     /locus_tag="LGK42_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBK2659952.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAVTRAPRKTRSVTPAKRGRPKKKAS"
     gene            5804..6313
                     /locus_tag="LGK42_003758"
     CDS             5804..6313
                     /locus_tag="LGK42_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBK2659953.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            6313..6516
                     /locus_tag="LGK42_003759"
     CDS             6313..6516
                     /locus_tag="LGK42_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBK2659954.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            6520..6801
                     /locus_tag="LGK42_003760"
     CDS             6520..6801
                     /locus_tag="LGK42_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2659955.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            6801..7298
                     /locus_tag="LGK42_003761"
     CDS             6801..7298
                     /locus_tag="LGK42_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HBK2659956.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            7313..7738
                     /locus_tag="LGK42_003762"
     CDS             7313..7738
                     /locus_tag="LGK42_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HBK2659957.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPKSVAVSFVAVWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTALFLQPVPWRRRAAALVVMVTTFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            7726..8169
                     /locus_tag="LGK42_003763"
     CDS             7726..8169
                     /locus_tag="LGK42_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBK2659958.1"
                     /translation="MSKLMTVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCPQRLPESESLPDAGQRPGDER"
     gene            8138..8296
                     /locus_tag="LGK42_003764"
     CDS             8138..8296
                     /locus_tag="LGK42_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HBK2659959.1"
                     /translation="MPGSDPETNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            8259..8726
                     /locus_tag="LGK42_003765"
     CDS             8259..8726
                     /locus_tag="LGK42_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2659960.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPVELYINGELVSKWDE"
     gene            8719..9171
                     /locus_tag="LGK42_003766"
     CDS             8719..9171
                     /locus_tag="LGK42_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBK2659961.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEKIILAHLER"
BASE COUNT         2048 a         2517 c         2736 g         1980 t
ORIGIN      
        1 tcagtgtcca gtgagtagtt tttaaagcgg atgacctcct gaccgagcca gccgtttatc
       61 tcgcggatcc tgtcttgtag tgggattagc tcattgcgga caaagacctt tgccactttc
      121 tcaatatcac ccagcgaccc gacgttctcc ggcttgccgc ccatcaactg aaaggggatg
      181 cggtgtgcgt ccagcaggtc agcggcgctg gcttttttga tattaaaaaa atcgtccttc
      241 gttgccactt cactgagcgg gataatttta atgccgtcgg ctttcccctg cggggcatag
      301 agaaacagat ttttaaagtt gttgcggcct ttcgacttca ccatgttttc gcgaagcatt
      361 tcgatatcgt tgcgatcctg cacggcatcg gtgacgtaca tgatgtatcc ggcatgtgcg
      421 ccattttcgt aatacttgcg gcggaacaac gtggccgact cattcagcca ggcagagtta
      481 agggcgctga gatattccgg caggccgtac agctcctgat taatatccgg ctccagcagg
      541 tgaaacacgg agccgggcgc gaaaggtgtc ggctcgttga aggacggcac ccaccagtaa
      601 acatcctcct ccacgccacg gcgggtatat tttgccggtg aggtttccag tctgatgacc
      661 ttaccggtgg tgctgtatcg cttttccaga aacgcattac cgaacaccag aaaatccagc
      721 acaaagcggc tgaaatcctg ctgggacagc cacggatgcg ggataaatgt cgaggccaga
      781 atattacgtt tgacgtaaat cggggagctg tgatgcacgg cagcacgcag gctttttgcc
      841 agaccggtaa agctgaccgg cggctcatac catctgccgt tactgatgca ctcgacgtaa
      901 tccagaatgt cacggcggtc gagtaccggc accggctcac caaaggtgaa tgcctccatt
      961 ttcggggcgc tggcggtcat tgtttttgcc gcaggttgcg gtgttttccc ttttttcttg
     1021 ctcatcagta aaactccaga atggtggatg tcagcggggt gctgataccg gcggtgagtg
     1081 gctcatttaa cagggcgtgc atggtcgccc aggcgaggtc ggcgtggctg gcttcctcgc
     1141 tgcggctggc ctcataggtg gcgctgcgcc cgctgctggt catggtcttg cggatagcca
     1201 taaacgagct ggtgatgtcg gtggcgctga cgtcatattc cagacagcca cggcggataa
     1261 cgtcttttgc cttgagcacc attgcggttt tcatttccgg cgtgtagcgg atgtcgcgtg
     1321 cggcgggata gaacgagcgc acgagctgga acacgccgac accgaggccg gtggcatcaa
     1381 taccgatgta ttcgacgttg tatttctcgg tgagtttgcg gatggattca gcctgagtgg
     1441 caaagtccat gcctttccac tggtgacgct caagtattct gaatttgcca ccggccacca
     1501 ccggcggtgc cagcaccacg catccggcac tgtcgccacg gtgtgacggg tcgtaaccaa
     1561 tccagaccgg gcgggagccg aacggattgg cggcaaacgg cgcatagtct tcccattctt
     1621 ccagcgtgtc gaccatgcag cgttgcagct cctcgaacgg gaacaccgat gccttgtcgt
     1681 caacaaattc acacatgaac aggtttttaa aatcgtcggc gctgttttcg cgtttgagct
     1741 gctcaatgtc gaacagcgtg cagccgcctt tcagggcgtc ctcaatggtg acaatctgtc
     1801 gccactggcc gtccgcacag agaagacctc cggcaagggc gttatgactg acgtcgattt
     1861 ccacgcgttc ggcggcgctg gcgcgtcccc ggttgaacag ttcacccgac cagaacgggt
     1921 aggcgtcgtg cgccagcgtg gacggggtgg agaaataggt cgagcgcagg tgactctgtg
     1981 aggccatacc tgatgccacc ttacgcagta cctgaaaatt cgggatccag aaaatctcgt
     2041 cgacgtacag gtcgccgtta tggctctgtg cggtgttgga gttggtgccg agaaaaatca
     2101 gttttgcgcc gttattgccc aggacaatcg ggtcaccggt aaggtcaacg tcaaccagac
     2161 gggcaaaggc gatgatgtat tcgcggaaca catacgcctg cgttttactg gccgacagaa
     2221 aaatctggtt atgaccggtt ttcagggcgc gcagcagcgc ctcgcgggaa aaataaaacg
     2281 ttgcgccaat ctggcgggat ttcaggatat cgcggatgcg gtgctcaagc ccggcgcgat
     2341 accagtgcaa ctgatagtcg aaagactgct caaagaaaat ctgctccagc ttttcgatgg
     2401 cctcgtcact gaaaaaattc tttttcggtt tgcgacgccc gcctttgttg cggttagcga
     2461 cgttcggatt aaggtctgct tcgttgccgg tctgactgta gcggttgacc cgtgccagtc
     2521 gttcaatctg gcgtccgagc aggtcaattt ccttgaagtc accgccggtt ttctgcggtt
     2581 tgatgatgag ctgggtcagc cgcgcttcca gactcatttc gacacggctg atgggggcaa
     2641 cgctgtccca gccgtcgcgc tgtttccagc tctgcaccgt cgggcgtttc atctgcaaca
     2701 tggcggcaat ctgcggcacg gaaaacccct gccagtacag cagcgccgcc tgacgacgcg
     2761 ggtcgtgtaa aagagtggtg tctgtggtga tggtcatgaa tacctcgccg tgatgaatac
     2821 acggcaaggc tactgagtcg cgccccgcga ttcgctaagg tgctgttgtg tcagtgataa
     2881 gccatccggg actgatggcg gaggatgcgc atcgtcggga aactgatgcc gacatgtgac
     2941 tcctctactc actattcagg actcctgaca atggcaaaaa aagtctcaaa attctttcgt
     3001 atcggcgttg agggtgacac ctgtgacggg cgtgtcatca gtgcgcagga tattcaggaa
     3061 atggccgaaa cctttgaccc gcgtgtctac ggttgccgca ttaacctgga acatctgcgc
     3121 ggcatcctgc ctgacggtat ttttaagcgt tatggcgatg tggtcgaact gaaggccgaa
     3181 aagattgacg atgattcggc gctgaaaggc aaatgggcgc tgtttgcgaa aatcaccccg
     3241 accgatgacc ttatcgcgat gaacaaggcc gcgcagaagg tctacacctc aatggaaatt
     3301 cagccgaact ttgccaacac cggcaaatgt tatctggtgg ggctggccgt caccgatgac
     3361 ccggcaagcc tcggcacgga atacctggaa ttctgccgca cggcaaaaca caaccccctg
     3421 aaccgcttca aattaagccc tgaaaacctg atttcagtgg caacgcctgt tgagctggaa
     3481 tttgaagacc tgcctgaaac cgtgttcacc gccctgaccg aaaaggtgaa atccattttt
     3541 agccgcaaac aggccagcga tgacgcccgt ctgaatgatg tgcatgaagc ggtgaccgct
     3601 gttgctgaac atgtgcagga aaaactgagt gccactgagc agcgcctcgc tgagatggaa
     3661 accgcttttt ccgcacttaa gcaggatgtg actgacaggg cggatgaaac cagccaggca
     3721 ttcacccgcc tgaaaaacag tctcgacagc accgaaagtc tgacccagca gcgccgcagc
     3781 aaggccaccg gcggtggcgg tgatgccctg atgacgaact gctgaccggc gtcagtcagt
     3841 ccgggaaaac cttcacgatt aactcttaat ttcaggaaaa actatgcgcc aggaaacccg
     3901 ctttaaattt aatgcctacc tgtcccgtgt tgccgaactg aacggcatcg acgccggtga
     3961 tgtgtcgaaa aaattcaccg ttgaaccgtc ggtcacccag accctgatga acaccatgca
     4021 ggagtcctct gactttctga cccgcatcaa tattgtgccg gtcagcgaaa tgaaagggga
     4081 aaaaattggc atcggtgtca ccggctccat cgccagcacc accgacaccg ccggtggcac
     4141 cgagcgtcag ccgaaggact tctcgaagct ggcgtctaac aagtacgaat gcgaccagat
     4201 taacttcgat ttttatatcc gctacaaaac gctggacctg tgggcgcgtt atcaggattt
     4261 ccagctccgt gtccgtaacg ccattatcaa acgccagtcc cttgatttca tcatggccgg
     4321 ttttaacggc gtgaagcgtg ccgaaacctc tgaccgcagc agcaatccga tgctgcagga
     4381 tgtggcggtc ggctggctgc agaaataccg caatgaagcc ccggcgcgcg tgatgagcaa
     4441 ggtcactgac gaggaaggcc gcaccacctc tgaggttatc cgcgtgggca agggcggtga
     4501 ttatgccagc ctcgatgcac tggtgatgga tgcgaccaac aacctgattg aaccgtggta
     4561 tcaggaagac cctgaccttg tggtgattgt ggggcgtcag ctactggcgg acaagtattt
     4621 ccccatcgtc aacaaggagc aggacaacag cgaaatgctg gccgctgacg tcatcatcag
     4681 ccagaaacgc atcggtaacc tgccggcggt acgcgtcccg tacttcccgg cggatgcgat
     4741 gctcatcacg aagctggaaa acctgtccat ctactacatg gatgacagcc atcgccgcgt
     4801 gattgtggaa aacccgaaac tcgaccgcgt ggagaactac gagtcaatga acattgatta
     4861 cgtggtggaa gactacgccg ccggttgtct ggtggaaaaa atcaaggtcg gtgacttctc
     4921 cacaccggct aaagcgaccg cagagccggg agcgtaaccg atgacgagtc ccgcacagcg
     4981 ccacatgatg cgggtctcgg cagcgatgac cgcgcagcgg gaagccgccc cgctgcgaca
     5041 tgcaactgtc tatgagcaga tgctggttaa gctcgccgca gaccagcgca cactgaaagc
     5101 gatttattca aaagagctga aggccgcgaa aaagcgcgaa ctgctgccgt tctggttgcc
     5161 gtgggtgaac ggcgtgctgg agcagggcaa aggcgcacag gatgacattc tgatgacggt
     5221 catgctgtgg cgtctggata ccggcgatat tgccggtgcg ctggagattg cccgttacgc
     5281 cctgaagtac ggtctgacca tgccgggtaa acaccgccgc accccgccgt acatgttcac
     5341 cgaggaggtg gcgctcgcgg ccatgcgtgc tcacgctgcc ggtgagtctg tggatacccg
     5401 cctgctgacg gagaccctcg aactgaccgc cacggctgac atgcctgatg aagtgcgcgc
     5461 aaagctgcac aaaatcaccg gtctgtttct gcgtgacgct ggtgatgccg ccggtgcgct
     5521 ggcgcacctg caacgtgcga cacagctcga ctgtcaggca ggcgtcaaaa aagagattga
     5581 acgactggag cgggagctga aaccgaagcc ggagccgcag cccaaagcgg tcacccgcgc
     5641 cccgcgtaag acacggagcg tgacaccggc aaaacgtgga cgcccgaaaa agaaagccag
     5701 ttaacaaccg aatgcgcccc gcgccagggc ggcacgccgg tcagtgaggg tgaatcacct
     5761 gacactgcac cggcgtccac cgcccgactt ttcagaggta gtcatgatga cgctgattat
     5821 tccgcgaaag gaggctcccg tgtccggtga gggtacggtg gtcatcccgc aaccggcagg
     5881 cgacgagccg gtgattaaaa acacgttctt ttttcccgat atcgacccga agcgcgtccg
     5941 ggaacgtatg cgccttgagc agaccgtcgc ccccgcccgt ctgcgtgagg ctatcaagtc
     6001 aggcatggcg gagacaaatg cggagctgta cgagtaccgc gaacagaaaa ttgccgccgg
     6061 ttttacgcgt ctggcggacg tcccggcgga cgacatcgac ggtgaaagca tcaaagtttt
     6121 ttactacgag cgcgccgtgt gtgcgatggc gaccgcgtcg ctttatgagc gttatcgcgg
     6181 cgtggatgcc agtgccaagg gcgacaaaaa ggccgacagc atagacagca ccattgatga
     6241 gctgtggcgg gatatgcgct gggcagtggc gcgcatccag gacaagccgc gctgcatcgt
     6301 gagtcaaatc tgatgaagac ctttgcgcta cagggcgaca cgctcgacgc catctgtgtc
     6361 cggtattacg ggcgcactga gggcgtggtc gaggccgtgc tcgccgcaaa tccgggactg
     6421 gctgaactgg gggcggtgct gccacatggc accgccgtcg aactgcccga cgttcagacc
     6481 gcgcccgtgg ctgaaactgt caatctgtgg gagtaacgca tgacagcaga agaaaaaagc
     6541 gtcctgtcgc ttttcatgat tggggtgctg attgttgtcg gcaaggtgct tgccggtggt
     6601 gaacccatca ccccgcgtct gtttatcggg cgcatgttgc tcggtggttt tgtctcgatg
     6661 gttgccggtg ttgttctggt gcagtttcct gacctgtcac tgcctgcggt gtgcggcatc
     6721 ggctccatgc tgggtatcgc cggttatcag gtgattgaga ttgccattca gcgccgcttt
     6781 aagggcaggg ggaaaccgta atgccggtaa ttaacacgca tcagaatatc gctgcctttc
     6841 tcgacatgct ggcggtgtcc gaagggacgg cgaatcatcc gctgacgaaa aaccggggct
     6901 atgacgtgat agtcaccgga ctggacggga agccggaaat tttcaccgac tacagtgacc
     6961 atccgttcgc gcatggccga ccggcgaagg tgtttaaccg tcgcggtgaa aaatccacgg
     7021 cctccggtcg ctatcagcag ctttatctgt tctggccgca ttaccgcaaa cagcttgccc
     7081 tgccggattt cagtccgttg tcacaggaca ggctcgccat tcagttgatc cgcgaacgcg
     7141 gtgcactgga tgacatccgg gcgggacgca ttgagcgcgc catttcacgc tgtcgcaata
     7201 tctgggcgtc cctgccgggt gccggttacg gtcagcgtga gcattcactg gaaaaactgg
     7261 tcaccgtctg gcgtaccgct ggcggcgtac cggcttaaac ggagtaaaca ccatgaagaa
     7321 attatccctt tcactgatgc tgaacgtgtc gctggcgctg atgctggcac tgtccctgat
     7381 ttacccgaag agcgtggccg tcagttttgt cgctgtctgg gcgattctgg cgacggttat
     7441 ctgtgtggtt gccggtggtg tcggcgtgta tgccacggag tatgtgctgg aacgctacgg
     7501 gcgggagctg ccgccggaat cgctggccgt gaagattgtc acggcgctgt ttttgcagcc
     7561 ggtgccgtgg cgcagacggg cggcggctct ggtggtgatg gtgacgacgt ttatctcgct
     7621 ggtcgctgcc gggtggattt ttaccgcgct gatttatctc gtggcatcgg tgttcttccg
     7681 gctgatacgt acggcctgcc gtcagcgttt tgaggggcgg gaaccatgtc aaagctgatg
     7741 actgtgctgg tcgtgttgtt atcgctggcg gtggccggtc tgtttctggt gaaacataaa
     7801 aatgccagcc tgcgcgcctc gctggacagg gcgaacaacg tcgccagtga acagcagacg
     7861 accatcacca tgctgaaaaa tcagcttcat gttgcgctca ccagggcaga caaaaacgag
     7921 ctggcgcagg tggctctgcg tcaggaactg gagaacgccg cgaagcgtga agcacagcgc
     7981 gagaaaacca tcacgaggtt acttaatgaa aacgaagatt ttcgccgctg gtacggcgct
     8041 gacctgcctg atgctgtgcg ccggttgcac cagcgccccg cctgcaccga cgccagtgat
     8101 tgtccacaac gcctgcccga aagtgagtct ttgcccgatg ccgggcagcg acccggagac
     8161 gaacggtgat ttaagtgccg atatccggca gcttgagaac gcgctggcgc gctgtgccag
     8221 ccaggtaaaa atgattaaac actgtcagga cgaaaacgat gctcaaaccc gacagcctgc
     8281 gcagggcgct gactgatgcc gtcacggtgc tgaaaactaa ccccgatatg ctgcggatat
     8341 tcgtggataa cgggagtatt gcctccacgc tggcggcgtc gttgtcattc gaaaagcgtt
     8401 acacgctcaa tgtcattgtg accgacttta ccggtgattt tgacctgctc attgtgccag
     8461 tgctggcgtg gctgcgggaa aatcagccag acatcatgac cactgacgaa ggtcagaaaa
     8521 agggcttcac gttttatgca gacatcaaca atgacagcag ctttgatatc agtatcagcc
     8581 tgatgctgac cgagcgcacg ctggtcagtg aggtggacgg cgcactgcat gtgaagaata
     8641 tcccggaacc tccgccgccg gagccggtca cccgcccggt ggagctttat atcaatggcg
     8701 aactggtgag caagtgggat gaatgagttt aagcgttttg aagaccggct gaccggactt
     8761 attgaatcgc tgtcaccgtc agggcgtcgg cgactgagcg ccgaactggc gaaacgtctg
     8821 cggcagagtc agcagcgccg ggtgatggca cagaaagccc cggacggcac accctacgca
     8881 ccacgccagc agcagagcgc cagaaaaaag acaggtcgcg ttaagcgaaa aatgtttgcg
     8941 aaacttatca ccagtcgttt tttgcatatc cgcgccagcc ctgaacaggc atcaatggag
     9001 ttttacggcg ggaagtcacc gaaaatagcc agtgtgcatc agttcggtct gtcggaagaa
     9061 acccggaaag acggtaagaa aattgattat ccggcgcgtc ctctgctcgg ctttaccggt
     9121 gaggatgtgc agatgattga aaagattatc ttggctcacc ttgagcgtta gttttatcca
     9181 ggcagaggct gatgcgcaat taaacattga gcggccatgc tggtcgctca atgtttagag
     9241 gtttatgaat gatttttatt tgatgctttg tattctaaaa c
//
LOCUS       DAFAVF010000162         9164 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFAVF010000162 DAFAVF010000000
VERSION     DAFAVF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.164"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..670)
                     /locus_tag="LGK42_003767"
     CDS             complement(<1..670)
                     /locus_tag="LGK42_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBK2659962.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVLRLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(913..1119)
                     /locus_tag="LGK42_003768"
     CDS             complement(913..1119)
                     /locus_tag="LGK42_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBK2659963.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            1432..1521
                     /gene="kdpF"
                     /locus_tag="LGK42_003769"
     CDS             1432..1521
                     /gene="kdpF"
                     /locus_tag="LGK42_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBK2659964.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1521..3194
                     /gene="kdpA"
                     /locus_tag="LGK42_003770"
     CDS             1521..3194
                     /gene="kdpA"
                     /locus_tag="LGK42_003770"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBK2659965.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            3217..5265
                     /gene="kdpB"
                     /locus_tag="LGK42_003771"
     CDS             3217..5265
                     /gene="kdpB"
                     /locus_tag="LGK42_003771"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBK2659966.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVNAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            5274..5846
                     /gene="kdpC"
                     /locus_tag="LGK42_003772"
     CDS             5274..5846
                     /gene="kdpC"
                     /locus_tag="LGK42_003772"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBK2659967.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5839..8523
                     /gene="kdpD"
                     /locus_tag="LGK42_003773"
     CDS             5839..8523
                     /gene="kdpD"
                     /locus_tag="LGK42_003773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBK2659968.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            8520..>9164
                     /gene="kdpE"
                     /locus_tag="LGK42_003774"
     CDS             8520..>9164
                     /gene="kdpE"
                     /locus_tag="LGK42_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBK2659969.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFIT"
BASE COUNT         1895 a         2423 c         2680 g         2166 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtctc aggacgccgc
      241 cgcgcaccag ccacagtgac tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga ggatgaacgg cagcggggcg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac cgagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccgatg cgcaccccgg
      601 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc gccctgacgc gccgccggtt
      661 ttccgctcat ctgctcatcc ttgttttatc gggttatttg tctgctgaca ggcgaaaccc
      721 tacagattta taagaaagcc ttaagtgaga tatctgtcag ataagaacaa ttaggaaaat
      781 atttatatat atttcatgaa tatatgtttc gattggtata acgatgtcta aatattcctc
      841 tgaacatctt cctgatacaa aaaaccgcca gcgtttccgc tggcggtttt caatgtattc
      901 aaaactcagc aatcaataaa aatcaccagt tgccttttcg gcctgatcca tccacaccgg
      961 tttatcgctg gttttcgacc agacgcgatg caggtagctg taaaagcggg cgcggtcttt
     1021 ccagaaaagc atgaaaggca gcgccagaac gcccgctgca accgcataag tacggcgtaa
     1081 aaagataagc catgcaggat attctctgta gagttccata cttttctccc ccttttgtat
     1141 ctacccggtg aatggcaccg gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct
     1201 tttgtgtaat tttactactc atccgaccac ttatttttgc ttattgatgg tttatttaca
     1261 ttcatcctgt aattaagtta cacaaaagtt aaattaatac taaacattag ttaaatcatg
     1321 gcttttgcca tttttatact ttttttacac cccgcccgca gatttttgcg aaatctttgc
     1381 agccagaatt ctacccttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca
     1441 ggcgtgataa ccggcgtatt gctggtgttt ttattactgg gttatctggt ttatgccctg
     1501 atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgatcgcc acgtttttac
     1561 tggtgttaat ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg attaatgaca
     1621 ttcctcttcc cggtacaacg ggcgttgagc gcgtactttt tcgcgcactt ggcgtctctg
     1681 accgtgagat gaactggaag caatatcttt gtgccattct cggcctgaac atgctggggc
     1741 tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt
     1801 tgccggggct gtcgtgggat ctggcgctga ataccgccgt cagctttgtc acgaatacca
     1861 actggcaatc ttacagcggt gaaaccacgt tgagctattt cagccagatg gctggcttaa
     1921 cggtgcaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc ctcatccgtg
     1981 cgtttacccg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg ctacgcatca
     2041 cgttatgggt gctcgtccct gtggcgttgt tgatcgcact gttttttatt caacaaggtg
     2101 cgctgcaaaa ctttcagcct tatcaggctg tgaataccgt tgaaggagcg caacagctgt
     2161 tacccatggg gcctgtagct tctcaggaag cgatcaagat gctcggtact aacggcggtg
     2221 gcttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg
     2281 tgcagatgct ggcgatcttc ttgatcccaa cggcgctgtg ctttgccttt ggtgaagtga
     2341 cgggcgatcg ccgccagggg cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct
     2401 gcgtaggcgt ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg gcgctcggcg
     2461 cagacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc
     2521 tgtttgcggt cgtgactacg gcggcttcct gtggcgcggt gattgcgatg catgattcgt
     2581 ttaccgctct cggtggcatg gtgccgatgt ggctgatgca aattggtgaa gtggtgttcg
     2641 gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg
     2701 ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgcgaga
     2761 tgaaactgac tgcactggcg attctggtta ccccgacgct ggtgctgatg ggcgcggcgc
     2821 tggcaatgat gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta
     2881 gcgaagtgct gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat
     2941 taagcgccaa ctccccgttc tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct
     3001 tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccaac
     3061 ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca
     3121 ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccggtggcgg
     3181 aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta
     3241 ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag
     3301 gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt
     3361 attagcatcg cgatggcaag cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt
     3421 agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc
     3481 cgcagtaaag cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag
     3541 ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa
     3601 ggtgacatcg tgctggtaga agctggcgat attatcccct gcgatggtga agtcattgaa
     3661 ggcggcgcat cggtcgatga aagcgccatc accggggaat cggcaccggt gatccgtgaa
     3721 tccggcggcg attttgcctc cgtcaccggc ggcacgcgta ttctttctga ctggctggtg
     3781 attgagtgta gcgttaaccc cggtgagaca tttctggatc ggatgatcgc gatggtggaa
     3841 ggcgcacagc gacgcaaaac accgaacgag attgccctga ctatcctgct gattgctctg
     3901 accatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat
     3961 gccgtgagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac cactattggc
     4021 ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt
     4081 gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct actggataaa
     4141 accggcacca tcacactcgg taaccgtcag gcgtcggagt ttatccctgc gcagggcgtg
     4201 gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa
     4261 ggccgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg cgatgtgcag
     4321 tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg gatcaacatc
     4381 gacaaccgca tgatccgtaa aggttctgtc aatgccattc gccgccatgt tgaggctaac
     4441 ggcggtcact tccctaccga tgttgatcaa aaagtcgatc aggttgcgcg tcagggggcc
     4501 acgccgctgg tggtggtgga aggttcccgt gtgctgggcg ttattgcgct gaaagatatc
     4561 gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgca aaatgggcat taaaacggtg
     4621 atgattaccg gcgataaccg tctgactgcc gccgccattg ctgcggaagc gggcgtcgat
     4681 gattttctcg ccgaagcgac accggaggcc aagctggcat tgattcgtca gtatcaggcg
     4741 gaaggccgtt tggtggcgat gaccggcgac ggcaccaacg atgctccagc gctggcgcag
     4801 gcagatgtcg cggtggcgat gaactccggt actcaggcgg cgaaagaagc gggcaatatg
     4861 gtcgatctcg actctaaccc gaccaagttg atcgaggtgg tacatattgg caaacagatg
     4921 ctgatgaccc gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc
     4981 gccattattc cggcggcgtt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg
     5041 cgccttcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc
     5101 gtctttttga ttcctctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc
     5161 atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt
     5221 atcaaagtca ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt accatgcgtg
     5281 gcttacgtcc ggcattatcg acatttatct ttctgttatt gattactggc ggcgtttacc
     5341 cgctgctgac caccgtgctg gggcaatggt ggtttccctg gcaggccaac ggttcgttga
     5401 ttcgtgaagg tgatacggtg cggggttcgg cattaatcgg gcagaatttt accggcaacg
     5461 gctattttca gggtcgcccg tcggcaacgg cagaaatgcc ctataatcca caggcttctg
     5521 gcgggagcaa tctggcggtc agtaaccctg agctggataa acaaatagcc gcacgcgttg
     5581 ctgcattacg ggccgctaac ccggatgcca gtacgaacgt tccggttgaa ctggtgacgg
     5641 catcggcaag cgggctggac aataatatca ccccgcaagc ggcggtctgg caaatcccac
     5701 gcgtggcgaa agcgcgtaat ctcagcgttg aacagctcac gcaactgatc gcaaaataca
     5761 gccaacaacc gctggtgaaa tatatcggcc agccggtcgt caacattgtt gaactcaatc
     5821 tggcgctgga taaacttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg
     5881 gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc
     5941 gtcgggaaga cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat
     6001 attgtggttg gcgtggtaga aacccatggg cggaaagata ccgccgccat gcttgaaggg
     6061 ctggctgttc tgccgcccaa acgccaggcg taccgtgggc ggcatatcag cgagtttgat
     6121 ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt
     6181 aatgcgccag gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct
     6241 ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc
     6301 agcggcgtca ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc
     6361 gacgacgtag tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc
     6421 aaagtctata ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat
     6481 ctgatcgccc tgcgtgaact ggcactgcgc cgtactgccg atcgcgtaga tgagcaaatg
     6541 cgcgcctggc gggggcatcc tggtgaagag aaagtgtggc acacgcgcga cgcgatcctt
     6601 ttatgcatcg ggcataacac cggcagtgag aaactggtcc gcgcggcggc gcggttggcc
     6661 tcgcgcctgg gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6721 ccggaaaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6781 gagacggcta cactttctga tccagcggaa gaaaaagcag tagtgcgtta tgcccgtgaa
     6841 cataatctcg gcaagattat tctcggtcgc ccggcctcgc gccgctggtg gcgtcgggaa
     6901 acgtttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6961 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag
     7021 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacttta
     7081 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     7141 ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc taccgtcatt
     7201 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc tgtctctgat
     7261 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     7321 actgcgggtg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     7381 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     7441 agcgaacaat ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7501 aacggtaaat tgcagccgtt aacacatccg caaggaatga ccccgtggga cgatgccatc
     7561 gcacagtgga gttttgataa aggcctgcct gcgggtgcgg gcaccgacac gttacccggt
     7621 gtaccgtacc agattttgcc gttaaaaagc ggtgagaaaa cctacgggct ggtggtggtg
     7681 gaaccaggga atctgcgtca gttgatgatc ccggaacagc agcgcctgct ggagacgttt
     7741 acgctgttag tcgccaatgc gtttgagcgg cttacgctga ccgccagtga agaacaggcg
     7801 cggatggcaa gcgaacggga acagatccgc aacgctctgc tggcggcgct ttcgcatgat
     7861 ttacgcacgc cgctcacggt gctgttcggt caggcagaaa tcttaacgct cgatctggca
     7921 agcgaaggat cgcctcacgc ccgccaggcc agcgagattc gtcagcacgt gctgaacact
     7981 acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg
     8041 aagaaagagt ggttaacgct ggaagaagtg gtcggcagcg cgctgcaaat gctggaaccg
     8101 ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac
     8161 gggccactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt
     8221 gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc
     8281 tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct
     8341 cgcgggaata aagagtcggc agtaccgggg gtagggcttg gactggcaat ttgtcgggcg
     8401 atagtggatg tacacggggg cactattacc gcgttcaacc gaccggaagg tggtgcctgt
     8461 tttcgtgtta cacttcccca gcaaactgcc cctgaacttg aagaatttca tgaggatatg
     8521 tgacaaacgt tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc
     8581 tggagggcga cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc ttgctggaag
     8641 cggctacccg taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga
     8701 ttgagtttat ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac
     8761 gcagcgaaga gagcgacaaa atcgccgcgc tggatgccgg agcggatgat tatctgagta
     8821 agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg
     8881 ccaccgccgc gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc
     8941 gcgtgattca ccggggtgag gaagaggtgc atctcacgcc gattgagttc cgcctgctgg
     9001 cggtgctgct gaataatgcc gggaaagtgc tcacccagcg ccagttactc aatcaggtgt
     9061 gggggccaaa cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac
     9121 aaaaactgga acaggatccc gctcgccccc gccatttcat tact
//
LOCUS       DAFAVF010000163         9083 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFAVF010000163 DAFAVF010000000
VERSION     DAFAVF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.165"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            577..1478
                     /locus_tag="LGK42_003775"
                     /pseudo
     CDS             577..1478
                     /locus_tag="LGK42_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            1482..1787
                     /locus_tag="LGK42_003776"
     CDS             1482..1787
                     /locus_tag="LGK42_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659970.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGW
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            1864..2547
                     /locus_tag="LGK42_003777"
     CDS             1864..2547
                     /locus_tag="LGK42_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBK2659971.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            2548..2769
                     /locus_tag="LGK42_003778"
     CDS             2548..2769
                     /locus_tag="LGK42_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659972.1"
                     /translation="MNYAGHKKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2663..3217
                     /locus_tag="LGK42_003779"
     CDS             2663..3217
                     /locus_tag="LGK42_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBK2659973.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWIPASKIEKETRMYGT
                     CETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQ
                     RIESGISSGVAMEIINNVRENRQVTVTAELLASLIQTAEQALWKREWAARDNGLAVPE
                     CVTRRQAVVNQARALLKNNTREND"
     gene            3262..4030
                     /locus_tag="LGK42_003780"
                     /pseudo
     CDS             3262..4030
                     /locus_tag="LGK42_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4570..4872
                     /locus_tag="LGK42_003781"
     CDS             4570..4872
                     /locus_tag="LGK42_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2659974.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDVGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            4919..5341
                     /locus_tag="LGK42_003782"
     CDS             4919..5341
                     /locus_tag="LGK42_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBK2659975.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            5338..5529
                     /locus_tag="LGK42_003783"
     CDS             5338..5529
                     /locus_tag="LGK42_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659976.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(5801..6049)
                     /locus_tag="LGK42_003784"
     CDS             complement(5801..6049)
                     /locus_tag="LGK42_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659977.1"
                     /translation="MSRDPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATEAARPER
                     SLQACGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            6561..6791
                     /locus_tag="LGK42_003785"
     CDS             6561..6791
                     /locus_tag="LGK42_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2659978.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            6843..8204
                     /locus_tag="LGK42_003786"
     CDS             6843..8204
                     /locus_tag="LGK42_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBK2659979.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            8252..8815
                     /locus_tag="LGK42_003787"
     CDS             8252..8815
                     /locus_tag="LGK42_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2659980.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDYILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(8900..>9083)
                     /locus_tag="LGK42_003788"
     CDS             complement(8900..>9083)
                     /locus_tag="LGK42_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HBK2659981.1"
                     /translation="ARQTGQHCSSQNQSSRKKKNSSSSSSSSSSRNNNISNRQMTGMK
                     KQKPPSERPRNERGGA"
BASE COUNT         1897 a         2493 c         2740 g         1953 t
ORIGIN      
        1 tctgattatt agtctgggac catggtccca ctcgtatcgt cggtctgatt attagtctgg
       61 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg atcccactcg
      121 tgttgtcggt ctgattatcg gtctgggacc acggtcccac ttgtattgtc gatcagacta
      181 tcagcgtgag actacgattc catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt
      241 aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt
      301 gtcctgctta tccacaacat tttgcgcacg gttatgtgga caaaatacct ggttacccag
      361 gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt gtgtcgctgt cgccggcctc
      421 ctcacccggt cacgtttcgt cgttcctcct ccacgcgctg cggcttcggg gccgcacctg
      481 cattcgtatg cggtcgcccg gttacaggtg cggcacggcc tgatggaggc cgcatgtgag
      541 aggagaattc ccatgccaaa ctggtgctcg aatcgtatgt atttttctgg tgaaccggca
      601 cagatcgctg agattaaacg actggccagc ggtgcagtca caccacttta tcgccgcgcc
      661 acaaatgaag gtattcagct gtttctggcc ggaagtgccg gacttctgca gaccactgaa
      721 gatgtgcggt ttgaaccatg ccccggactg acggctgccg ggcgtggcgt tgtatcgccg
      781 gagaatatcg cgttcacccg ctggctgaca cacctgcagg acggtgtact tctggatgag
      841 caaaactgcc tgatgctgca tgaactctgg ctgcagagcg gtactggccg gcgtcgctgg
      901 gaagaattac cggatgatgc cagggaaagc attaccgctc ttttcacccc aaaaagaggt
      961 gactggtgcg acatctggag taacgaggat gtatcggtgt ggtggaaccg tctgtgtgac
     1021 aacgtcctgc cggaaaaacc atgccgtttg acctgctgac ggttctgttc actcgcctgg
     1081 atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct gcatatcact
     1141 ggtatacgga acagtatggc gtgaaggggc cttgtgggta tgaggtgaat atcagcagtc
     1201 agggagacaa cttcattcag gttgatttcg acacgccgtg gtgtcagccg gaaagcgacg
     1261 ttattgcagt attaagccgc cgtttcagtt gcacgctgga gcactggtac gccgagcagg
     1321 gctgcaattt ctgtggctgg cagcgatatg agcgtggaga gcttgttgat gtgctgtggg
     1381 gggaactgga atggtcttcc ccgacagatg atgatgagct gccggaagtc accgcgccgg
     1441 agtggatagt cgacaaggtg gcgcattacg gtggatgaag tatgacggag actggcgggc
     1501 aaccgcccgt ttcttttccg gtaaaggatg tcgccgggct cctcttttta ctgcgccggc
     1561 tgacgcggcg cggcaggaac gctgcctgtg gtcagtgtcc cgcgtccggc tgggcacggg
     1621 acgggcgctt ttaccgctcc cggctggcat cggtgacagt gtacgccagc ccgtcgccct
     1681 tttctgatga acgcccttca agccgctttc gcggcatatt ctcgccgtca aaaagacgac
     1741 ggctgcgata ttccacggtc ggcctgaccc gttaccggac gcggtgaaca gcccacaggc
     1801 cgcggggaac gggcgccaca ggggcgccct cccggtgccc tctgtaagag aaggagtgct
     1861 gttatgtccc gttttatcca gggtgactgc gtgcgtgtta tggccacgtt tccgggcaat
     1921 gctgtcgatt tcatcctcac cgacccgcca tatctcgtcg gtttccgtga ccgtcagggg
     1981 cgtaccatcg ccggcgataa aaccgatgaa tggctgcaac cggcctgtaa tgaaatgtac
     2041 cgcgtgctga aaaaagatgc gctgatggtg agcttctatg gctggaaccg cgtcgatcgc
     2101 tttatggccg cctggaaaaa tgcgggattc agcgtggttg gccacctggt gtttaccaaa
     2161 acctacacat cgaaggccgc atatgtgggc tatcgccacg aatgcgctta catcctggca
     2221 aaagggcgtc cggcgctgcc tcagaagcca ttgcccgacg tgctgggctg gaaatattca
     2281 ggcaatcgtc atcacccgac ggaaaagcct gttaccagcc tgcaaccact gattgagagc
     2341 ttcacacatc cgaacgcgat tgtgctggac ccgtttgcag gcagcggctc aacctgtgtg
     2401 gccgcccttc agtccggacg ccggtatatc ggtatcgagc tgcttgaaca gtatcaccgt
     2461 gccggacagc aacgtctggc tgccgtacag cgggccatgc agcagggggc cgcaaatgat
     2521 gactggttta tgccggaggc tgcgtaaatg aactatgccg gacataaaaa acttcgcgcc
     2581 gacgtggcgg aggtggccaa taccatgtgt gaccttcggg ccaggctgaa cgatatggag
     2641 catcgctgtc gctttgattc cgatgtgctt gttgaacgcc tggcacgcca gacgctgtat
     2701 cgcgccaatc gcctgttcat ggaggcgtat accgaaattc tggaactgga ttcctgcttc
     2761 aaagattgag aaggagacga gaatgtacgg aacatgcgaa acgctatgcc gggagctggc
     2821 agcaaagtat ccgggagacg cgccgctgat gctggttatc tggtccccgg aagaaattca
     2881 ggccctcgct gacggaatgg atatttccct gtccgatcat gaaatcagga ccgtcctggc
     2941 gcggctggag gacatcccgg aagaccagcg gattgagtcc ggtatttctt ccggcgtggc
     3001 gatggagatc atcaataacg tgagagaaaa ccgccaggtg accgtcactg ctgaactgct
     3061 ggcgtccctg attcagaccg ccgaacaggc actgtggaaa cgtgagtggg ccgcccgtga
     3121 taacggcctc gccgtcccgg aatgcgtcac ccgccgacag gcggtggtta atcaggcccg
     3181 cgccctgctg aaaaacaaca cacgcgaaaa cgactgatgt tatcgccgcc tgcgggcggc
     3241 gattcaggga gaccatgaat catgaacgaa acgctgaatg cactgatttg tcgccacgcc
     3301 cgtagcctgc tgctggcgca gggctggccg gaagagacgg atgttgacca gcgaaatccg
     3361 aactatccgg gctggatcag catttacgta cggctggata ccccggctgg cgacgttact
     3421 cgttaaccgc cacgatggcg tgctgccgcc gcacctggcc tccgccattc agaaactgac
     3481 cggaaccggg gcggaactgg tactgtccgg cagtcagtgg cagtcgctgc cggtacttcc
     3541 ggcagacgga acgcaggtgt ctttctcgta tgccggagaa tggctgaccg aagacgaaat
     3601 cagggcagtt cttgatgcgg tgcgagatgc agtatgcagt gtcagttgcc gggtggcaga
     3661 agatgcgcgg cgtatccggg cggcgctgac cacaagcggt cagacgttgc tgacccgcca
     3721 gacgcgccgc tttcgcctgg tcgtgaagga aagcgatcac ccctgctggc tcgatgaaga
     3781 tgacgaaaac ctgcccgtgg tgctcgatgc catcgtgaac cggggagcac gtttttcgtc
     3841 ggtggaaatg tacctggtca gcgattgtat tgagcatatc ctgtccagtg ggctggcctg
     3901 cgatgtgctg cgtataccgg atgaaccgcc ccgccgctgg tttgaccgtg gtgttctgcg
     3961 ggaggtggtc cgggaagccc gggtagaaat ccgcagcatg gcggatgccc tggcaaaaat
     4021 ccggaaatga tgacactggc ggggtaaatc cccgcctttt tcctgccggt ccccggacag
     4081 agcacaaagg aagtcgccgc cggctccgct ttacccggcc atgcagggca tggccttgtg
     4141 gtttttcagt tatgtggcct cagcgtcgtg tgcgggctgt gccgtgcctc catcttagcc
     4201 gggctggcgt tgatgcaagg gtacgcttcg ccgctgcggt cacccggtcc ctccttcccg
     4261 tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc ctgcaactcc
     4321 cgtcagccgt gtgcgtcagg ctgcggcttc tcttgcgccc ctgcatcccc gcctgttcgc
     4381 ccggctttta tggaggcacg gcaccgcccg gtgtcgcgga atatgtgaat aacggaggaa
     4441 aacatcatgc aatatgcgaa acctgtcact ctgaacgttg aagagtgcga ccgtctctcc
     4501 tttctgcctt acctgtttgg caacgatttt ctgtatgccg aggcgtatgt gtacgcgctg
     4561 gcgcaaaaaa tgatgccgga atatcaggga ggcttctggc acttcatccg cctgccggac
     4621 ggtggcggtt acatgatgcc ggatggcgac cgttttcatc tggtcaacgg tgaaaactgg
     4681 tttgaccgca cggtcagcgc cgatgccgca ggcatcattc tgacttccct tgtgattaac
     4741 cgccagttgt ggctgtacca cgacagcggg gatgtaggac tgacccagct ttaccggatg
     4801 cgcgatgcgc agttgtggcg tcacatcgaa tttcaccccg aatgcaacgc gatttacgcg
     4861 gcactggact gattaacgga cggggcggca atgccgcccc tgtaaaggag acgagaacat
     4921 gtactgtact gttaaagaaa tcatccgcga tgtactggat acagacgtgc cggacagtga
     4981 atgcgttttt gccgtggtgc tgacccgtgg ggatgtgcgc cacatagccc aggactggaa
     5041 cctgtcagac gatgagctgg aaaccgtcat gcagcggctg gacgatgcct ttgaatatgg
     5101 tgcggatgtc agcattgttc acgacgttgt tcgtgagctg atggaagaaa agcgtgccag
     5161 ccgtcaggtg acagttccgg cggtgatgct ggaaaaagtg atggcgctgg caggcagtga
     5221 aatgaagcgc ctgtatgccg tcgggagtga gaacgggggc gacggtgatg cgttcgtcag
     5281 ggaggaacgc gaagcaatgg acgttgtgtt acaggcgctg gacggggagc acatgtcatg
     5341 aatatcagca cagaaacccg cgaaattctg cgcaattaca aagccgtgat taatgcgcga
     5401 cgtcgtgaaa tggggcagaa accgctcacc accgcgcaga ttgttgatga aatctgcgat
     5461 tttgtggcga atcagcaggc tgttttcctg ggcggtcact acatcttaca gggcagcaga
     5521 aacaggtgat acagggcagg cggtgaaggc cgcctgtcag cccgcgcgaa aacccctggc
     5581 aggctgacgc ctgccggaag ggaagcgggc cacgctggcg cgtcgcccgt tacccttccc
     5641 gtctgtgatg tgtctgactc aggagtgttt ctttcttatg ttatggaact gttcctgatg
     5701 ttgttgttcc tttatctgct gctctgattc tgactactgc agtgttgttc tgtctgccgg
     5761 gcgcaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga ctccgacagg
     5821 cagctgctgg cgcacctgcc agtctccgcc gaacccgctg gcgcggtttc gggctgtcgc
     5881 tacggtccga tgcgtgaaaa ccacaggctt gcagcgaccg ttccggtcgc gccgcctctg
     5941 tcgcgccgtg gcctgcgctc ccggcatccc ggcaaaaggc cgccgggatg taaggcgcaa
     6001 cgccgtttcg cgcacgcgct ctccgtcgtt gcggcgggtc cctgctcatt gcctgccggg
     6061 tgccacacac ccgtatgagt ccctgtcgcc gggggctgat acagccacaa cctgccgcac
     6121 tgactgtcca gggtaaatga acagcccaca aaatttttta cccaaaaccc ggtaaaaaat
     6181 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccct tgcggcgaag
     6241 gttctcaaac gggtgaagtt cataccacag ggaatacagc agcaggttcc cgcgaaacgt
     6301 caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg atttatttca
     6361 ggctccggca atatgcattt atttgttctg tcagtcatcc ggctggcagg taatgagaac
     6421 gcaggaacag aaggaggaaa acaccggaga acgtcagacg cagatatgaa aaaagcaggt
     6481 cagggacctg cttaatccac accgcaaaaa ctgttcgcgc agtttctgcg gttccattca
     6541 acagtcataa ggatgcattt atgacagtaa gtattgtttc accttccgca gcggctgtaa
     6601 agccccgccg tcatccccgt ttcagacgcg aagacatccc cgcgccggaa attgaccccg
     6661 tgctgaaagc cttcgggcga cacattgccc ggagttttca ccgggggcgt ggtgtacaca
     6721 tccccgccat gaaaaacacg gccttcgggc aggtgctccg cacactcgaa ctgaaacgcg
     6781 cttttaactg atgaggtgag ccgccccgtg aggggcggca acagacggaa ggagaacatt
     6841 ttatgacatc agcagcaatc accatgaccg ccccggaagc cgccagccct gtgcagatgt
     6901 accgcgcgac ctactcaccg gatgacaaca aactgcgcct gtatgccgtg tcacgtcttg
     6961 acccggagac gtataaaaaa gtgcatgatg ctggttttcg ctgggcacca aaacaggcgc
     7021 tgtttgtcgc gccagcctgg acaccgggcc gggaagacgt gctcctctca cttgccggag
     7081 agattgagga tgaagacagc acgctcgctg aacgtcagga agcacgggcg gagcggttta
     7141 ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa gtggaaagac
     7201 tggccgcgat gatcccgccc ggtcagccca ttcttgtggg gcatcacagc gaacgccgcg
     7261 cccgtcgtga tgcgcagcgt attgaaaacg gcatgaaacg agccgtgatg ctctttgaac
     7321 gtgcggaata ctgggaagag cgggcgcggt cggcactgct tcacgcgaag tataaagaac
     7381 gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc aaggctgaaa
     7441 agaccatcgc gcagtcgcag aaatatctga cgatgtggcg ggctgaatcg ctggatctga
     7501 atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg ctggatacgt
     7561 atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg atcgttatgg tcggccctgg
     7621 atgatgacat catcaccacg gagcaggccc gcgaaattgc gatccgctgt catgagcggc
     7681 agattcagca tcaacaacgc tgggttaacc actatcagaa ccgcctgatc tatgagcgtg
     7741 ccatgctgga cgaaagcggc ggcgtggtta cccggacaca ggattttgag ccgggcggac
     7801 aggttttcag ccggggcgag tggctgacca tcatccgtgt gaacaaaagc aacggggcgg
     7861 tgagttcagt cacaacgcca aattacagtt ttcttgggta cagcggcacg atgaaagtga
     7921 cgcccgatcg catcacggac tacaaagcac catcggcaga agaggctgcc gtcgccagtc
     7981 aggccgcgaa gcgtccgccg gtagtcaact atccggggga aggtttccgg gaaatgacaa
     8041 aggcacagtg ggccgccctg ccccgggact gtaaggccgt gcgtagtgtg gcagaagcag
     8101 aagaccacgg ggcataccgg taccgccgca caatggacaa taatttccgc ctggtgaatg
     8161 tgtatatcac cgacatgaaa atcacggaaa tcccacagaa ataaggtata tccccccggg
     8221 aaaatccccg ggggatgacg gagataatga tatgcacagt cagttaagag aacgtatccg
     8281 gctgatgcgc gcaaggctgg ataacgccgc gccggttgct gaaatccggg ctgaatctca
     8341 gctttttgtg actcctgccc cggtctgtga tcgcctggtg atgctggcgg agatcagcaa
     8401 ccgtgattac attctggaac cctctgccgg caccggggcc atactgcggg cgattcggga
     8461 taccgcgccg ggggccatgt gtgatgcggt ggaaatcaac agcgggctgg ttcggtatct
     8521 gcgggaaaat tttaacggtg tcagggttca gtgtggcgac ttcatggaat ggcagccggt
     8581 gcagtattac agccgggtta tcatgaatcc gccgttcagc cacgggcagg atatccggca
     8641 tatcctgcgg gccttttccc tgttgcgtcc gggtggcgtg ctggtcgccg tctgtctcaa
     8701 cggaccacgc cagcaggaga agctgttacc gttttctgac gtccgcgagg agctgccgcg
     8761 cggcacgttt gcttataccg atgtcccgac gatgattatt cgtctgcgtg cctgatgaca
     8821 cagggccggc gggaggataa atgcctgtct gcccgcgccg ggaaaaaacg cgcgccggtg
     8881 gcgctgaaag gggccgcgct cacgcgccgc ccctttcatt acgcggacgt tctgatgggg
     8941 gcttctgttt cttcatgcct gtcatttgtc tgttactgat gttgttgttc ctgctactgc
     9001 tactgctact gctactgcta ctgttcttct ttttcctgct gctctgattc tgactactgc
     9061 agtgttgtcc tgtctgccgg gcg
//
LOCUS       DAFAVF010000164         8894 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFAVF010000164 DAFAVF010000000
VERSION     DAFAVF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.166"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            102..1250
                     /locus_tag="LGK42_003789"
     CDS             102..1250
                     /locus_tag="LGK42_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2659982.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            1460..2890
                     /locus_tag="LGK42_003790"
     CDS             1460..2890
                     /locus_tag="LGK42_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK2659983.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNIIWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(2891..3799)
                     /locus_tag="LGK42_003791"
     CDS             complement(2891..3799)
                     /locus_tag="LGK42_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2659984.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYEQLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            3899..4489
                     /locus_tag="LGK42_003792"
     CDS             3899..4489
                     /locus_tag="LGK42_003792"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HBK2659985.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQIGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(4571..5311)
                     /gene="pflA"
                     /locus_tag="LGK42_003793"
     CDS             complement(4571..5311)
                     /gene="pflA"
                     /locus_tag="LGK42_003793"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBK2659986.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(5503..7785)
                     /gene="pflB"
                     /locus_tag="LGK42_003794"
     CDS             complement(5503..7785)
                     /gene="pflB"
                     /locus_tag="LGK42_003794"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK2659987.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(7840..8697)
                     /gene="focA"
                     /locus_tag="LGK42_003795"
     CDS             complement(7840..8697)
                     /gene="focA"
                     /locus_tag="LGK42_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBK2659988.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         2167 a         2046 c         2231 g         2450 t
ORIGIN      
        1 ataataccgc cgctgggata tcgcgtattt tcacccattg acaatgtttt tggcggtggc
       61 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
      121 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgattgcg gtgttaaata
      181 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
      241 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac
      301 gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
      361 gccttggcct gatgattggt ttctggagct ggttggcgtg gcgttttgtc gctggcgtcg
      421 gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta
      481 accgtgggcg tttgcttgct gcatacatga tggtttatta cgtgggaacg tttttaggcc
      541 agttactggt cagcaaggtc tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt
      601 tgacattggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa
      661 accatgattc gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg cttggcgtga
      721 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg ccgttgtacc
      781 tcaatcacaa aggggtgagc aatgccagta ttggtttctg gatggcggta ctggtcagtg
      841 cgggtatcct cggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg
      901 tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg
      961 cgatggcccc tgcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
     1021 tggcctgggc ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac
     1081 tgttgagcta taccgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
     1141 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
     1201 tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
     1261 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
     1321 tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg
     1381 ctatttccgc acttcttatt tgccgcgcat aatcccacgt tttacagatg cccctttaat
     1441 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagctgc gatggtacaa
     1501 cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta ataactatgc
     1561 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcactct atttcacacc
     1621 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
     1681 tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt ggacctactg
     1741 ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg cgctcggttg
     1801 ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt tacaggggtt
     1861 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
     1921 aatcgtcggt tctgtggcag ggattgcgat gtttgttatg tcgctcctgt atgtggcgat
     1981 ggcggtaacc gcgcctgcga ttactgaagt gcatattgcg accacaaaca ttatctggga
     2041 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
     2101 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
     2161 atttccaaaa gggatgctat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc
     2221 actggcgatg gggatgatgt ttgactcgcg taatatcccg gatgacttaa tgactaacgg
     2281 tcaatattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
     2341 gatttacgcc attgcgaata ccctgggaca agtagctgcg ctggtattct cgattgatgc
     2401 cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca gtttatgtcg
     2461 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtgc tggtggcaat
     2521 tctgattatg ctgccgactc tcggcatcgg tgatatgaac aatctctata aatggctgtt
     2581 gaatcttaac tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
     2641 agtcgttcgc ctggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct
     2701 ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt tgacggggat
     2761 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
     2821 aacgccgttt gtgctggtag gattaggact gattttcccg ctgctggcgc gtaaagcgaa
     2881 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
     2941 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa tagatctgga
     3001 agcctggacg ctctgtgctc cagtcttcca gtacacattc taaacgacca gtatcaaggt
     3061 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcctcca
     3121 gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc agctcctttg
     3181 caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat tgatgattaa
     3241 gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc gttgctgcta
     3301 cacataattt cactggcggg ccgatcgcca cgctgatcat atctttttcg acgatgccac
     3361 taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagag tcgtcagttg
     3421 tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa ggcattaaaa
     3481 atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt gtcagacgaa
     3541 aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc aattgttcat
     3601 agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg aataagcgaa
     3661 ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag gataagccta
     3721 attcatcgcc cgctgcacga aagctttgat tacgggccac ggcaaagaaa gtggcaaagt
     3781 cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa tcatcacgtt
     3841 tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag gtaaaattat
     3901 gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat tgaccgcaca
     3961 tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga cggaactcga
     4021 tttatatcgg cgtaatttca acccggtgat gacgtcggaa gatgaaccag actggaagaa
     4081 tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc ttgagcatga
     4141 cacgttagtg gtggtttttc ctctctggtg gtacagcttc ccggcaatgc taaaaggata
     4201 tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat taccattcaa
     4261 taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc agataggctg
     4321 ggaaaaaaat ataagcgatt atttaaaaaa tatgtgcagt tatcttggta ttgaagatgc
     4381 cgatgtcact ttcttgtgta atacagtggt attcgatggg gaagaacttc acgcgagcta
     4441 ttatcagtcg ttattatctc aggtacggga tatggtagat gcactataag atgtgttaaa
     4501 aacgctgtag cagaatgaag tgcggaataa aaaagcggca actcaataaa gttgccgctt
     4561 tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct ttcacgcgtt
     4621 ccatggtctc tttcttcggt ggtttaacac cgtcgagttt gtactcttca cccattgcca
     4681 cccatttgtg tttgcccagc tcgtggtagg ggagaagctc gattttctca acgttgccca
     4741 tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac cagcctggga
     4801 caacaacgta gcggatccac accttcacat ttttgttcgc cagatatttg gcgaactcca
     4861 gcgtgcggtg attggaaact ccaaccagat tttggtggat ctcgtcgttc atctgtttga
     4921 gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga tcgtaacgac
     4981 gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag gcgcggaacc
     5041 agtcacgaac aaactcagct tgtaggattg cctcaccgcc ggatgcggta acgccgccgc
     5101 cggaagcgtt cataaagtgg cgataggtca ccacctcttt cattaaatct tcaacggtaa
     5161 cttctttacc gccatgcgta tcccaggtgt cgcggttatg acaatacagg cagcgcatca
     5221 ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc gtctacggtt ccacaggatt
     5281 caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg tggcccatct
     5341 gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa
     5401 agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt tcgtggagcc
     5461 tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag attgagtgaa
     5521 ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac gtactgcgta
     5581 gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg cgtcgagcag
     5641 catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg atgcttcgtg
     5701 gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt cgtctttacc
     5761 cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt aagcaaacgg
     5821 cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac cgtgcatcgg
     5881 gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt taccagtttt
     5941 cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga tagcgtcacg
     6001 gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa cagccaggtc
     6061 atctacacgc ggatcattgt taccaaactg cgggtattcg ccttcgattt cgaagtcgat
     6121 agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg cagacaggga
     6181 gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa cgtcacggtc
     6241 gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt ggatgatgtt
     6301 cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc gctccatcac
     6361 ttcatcatag ttcaggacat cgcctttgat cggttcagac ttcggaccaa cctgcatttt
     6421 cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca ggtttgcacg
     6481 cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc aagcaatagc
     6541 gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact gcagagaaga
     6601 ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt tttcagacca
     6661 cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt tcaggaaacg
     6721 gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga tagattcggt
     6781 tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca ggaagcgaac
     6841 catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt cggtgatctt
     6901 gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac gaccgaagga
     6961 cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag tccactggat
     7021 agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg cagccatttc
     7081 tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac ggatagtctg
     7141 ttccaggttt acgccgtttt ccagatcagc ctgcagagaa gtgaactgtg ccagtttgtc
     7201 tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga tgatacggcc
     7261 acggccatat gcatctggca gaccggtcag aacaccagat ttacggcaac gcaggatgtc
     7321 cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag tgaagatttt
     7381 tttgatcatc ggatccagtt cgcggttgta cgctttgcag gaaccttcga tcattttgat
     7441 accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac caacgatttt
     7501 ctcaagctgc ttgttgatgt agccagcgtc gtgagaggtg atggtggaag caacagcggt
     7561 gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca ttactttgtc
     7621 ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac cctcgtacgg
     7681 agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt cacctttggt
     7741 aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac acctaccttc
     7801 ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtgat cgttttcacg
     7861 caggtaaatg acccagtatg tcaacccaac caacaaacca ccgccgataa tattaccgat
     7921 cgtaaccgga atcaggttat cagtgatgaa gttcatcacg gtcaggtgag aaaaattttc
     7981 cggtgcagaa ccgacagcgg tccagaattc cggagatgcg aagtcgcgga ttacaatacc
     8041 catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc
     8101 gaccggcagc accataatga acgctttgtc catcaggcta cggccagaat aactcatcca
     8161 tactgccaga cataccatca ggtttgccag gataccaagg cagacggcct caataaaagt
     8221 atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac catttgcggt
     8281 catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga ccaggttgcc
     8341 aaaatagaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc cactcgcctt
     8401 agcaacaaca atcaacactg tggaagtaaa gagatcggct ccgcagacaa cacaaagaat
     8461 cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg gcattgtgcc
     8521 tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa caccggcggt
     8581 aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac ccgcctcttc
     8641 ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag ctttcacact
     8701 aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga tgagaaattg
     8761 atatagatca tatctcgcct ggcttatagg cccgtaactc gcatggtttt tatgcaaata
     8821 cggagtaaat atttgattat ccaaataaaa ataaatttta aaaattaaca aatgagttga
     8881 attttttccg catc
//
LOCUS       DAFAVF010000165         8829 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFAVF010000165 DAFAVF010000000
VERSION     DAFAVF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.167"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..270
                     /gene="rcsA"
                     /locus_tag="LGK42_003796"
     CDS             <1..270
                     /gene="rcsA"
                     /locus_tag="LGK42_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBK2659989.1"
                     /translation="DILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQM
                     NIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(314..502)
                     /gene="dsrB"
                     /locus_tag="LGK42_003797"
     CDS             complement(314..502)
                     /gene="dsrB"
                     /locus_tag="LGK42_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBK2659990.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            665..892
                     /gene="yodD"
                     /locus_tag="LGK42_003798"
     CDS             665..892
                     /gene="yodD"
                     /locus_tag="LGK42_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBK2659991.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1189..2004
                     /locus_tag="LGK42_003799"
     CDS             1189..2004
                     /locus_tag="LGK42_003799"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBK2659992.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASGGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(2001..3695)
                     /gene="yedQ"
                     /locus_tag="LGK42_003800"
     CDS             complement(2001..3695)
                     /gene="yedQ"
                     /locus_tag="LGK42_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBK2659993.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKMCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(3933..4115)
                     /locus_tag="LGK42_003801"
     CDS             complement(3933..4115)
                     /locus_tag="LGK42_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBK2659994.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(4194..5111)
                     /locus_tag="LGK42_003802"
     CDS             complement(4194..5111)
                     /locus_tag="LGK42_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBK2659995.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5284..6204
                     /gene="yedA"
                     /locus_tag="LGK42_003803"
     CDS             5284..6204
                     /gene="yedA"
                     /locus_tag="LGK42_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBK2659996.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(6193..6663)
                     /gene="vsr"
                     /locus_tag="LGK42_003804"
     CDS             complement(6193..6663)
                     /gene="vsr"
                     /locus_tag="LGK42_003804"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBK2659997.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6644..8062)
                     /gene="dcm"
                     /locus_tag="LGK42_003805"
     CDS             complement(6644..8062)
                     /gene="dcm"
                     /locus_tag="LGK42_003805"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBK2659998.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8129..8566)
                     /locus_tag="LGK42_003806"
                     /pseudo
     CDS             complement(8129..8566)
                     /locus_tag="LGK42_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            8622..>8829
                     /locus_tag="LGK42_003807"
     CDS             8622..>8829
                     /locus_tag="LGK42_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBK2659999.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRAS"
BASE COUNT         2126 a         2373 c         2165 g         2165 t
ORIGIN      
        1 gatattctga aaaaagagac aacgataacc tcttttttaa atatgccgac gttatcattg
       61 agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc
      121 tctgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaagcgt
      181 aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg
      241 actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc
      301 aggctgaata agattactct gctttctcca caaagatacc gtcctgatgc cctgcttcat
      361 taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt gtgccttcac
      421 taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc gttttgactg
      481 ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat
      541 ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg gtctatactt
      601 aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa
      661 gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt
      721 cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa
      781 cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga
      841 tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa
      901 taaaaaaatc ccgaccctga ggggtcggga tgaaacttgc ttaagcaaga agcacttaaa
      961 aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atccgcaatt tttttcgctg
     1021 acaagaaaat attcgcgata tgaatgatta acgctactta tgaatagaaa tgtgacgtgc
     1081 gtcacccctt ggtggctctg gcgtcaacgg gatcgctccc gaaaaaatca tctttgatta
     1141 tttgtgctgt tttgagaatc atcctggtaa ggggagaact gcgcgaccat gttttcaatt
     1201 caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag tcatagttat
     1261 gactggcaac cggctgcccc ctggctcagc cgtttacgcg aagcgaatgt tcccgtcatt
     1321 ctctgtagca gtaaaacttc agcggaaatg ctgtacttgc aaaaaacgtt ggggctacaa
     1381 gggttaccgc tgattgccga aaatggcgca gtgatccagc ttgctgaaca atggcaggat
     1441 atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatcag ccaggtttta
     1501 aatacgctac gcgagaaaga acattttaaa ttcacgactt tcgatgatgt cgacgatgca
     1561 accatcgccg aatggacggg attaagccgt agccaggcgg cgctgacgca gctgcatgag
     1621 gcgtcggtaa cgctaatctg gcgcgacagt gacgagcgta tggcacaatt taccgctcgt
     1681 ctgaacgaac tgggcttaca gtttatgcag ggtgcgcgct tctggcacgt actggatgcc
     1741 tctggcggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca attgtcaggc
     1801 aaacgcccaa ccacacttgg cctgggcgat gggccaaacg atgcgccctt actggaggta
     1861 atggattacg cggtgattgt gaaagggcta aaccgtgaag gggtgcatct gcatgatgag
     1921 gatccggccc gcgtctggcg aacgcagcgt gaaggaccgg aaggctgggg tgaagggctg
     1981 gaccattttt tctccgcccg ttaagcgtta tcgctcgcgc atacccgatt acgcccggcc
     2041 tgtttagcga gataaagtcg acggtcagcc agtgactgga gttgttcaaa atcataatca
     2101 ccggtttcct cgctgctact tacccccaac gaggcactga tgcgtatcgt cgtactctta
     2161 gcaatcaaca tctctttttc atttaacttc aggcgaatac gttctgcgac ttccgcagcc
     2221 tccgtcagac tcgcgcctgg cagaatcaca caaaactcct caccaccgac ccgcccggca
     2281 acgtcctgcg cacgcaagga actgctaatt aatccggcag catgagaaag aacacggtcg
     2341 cccgcctgat gaccaaagcg gtcattaatc gctttgaaat ggtcaagatc gacctgaatg
     2401 acagaaaaag gatgttggtg cgtctgacac attttcgcga gcggacgggc tttttcgaac
     2461 agtgcgccac ggttatataa acgcgttaag gtgtcgtgcc acgcctgcca ctgcaacgag
     2521 ctttgcagaa catacatgtt gctgaccatc cggcgaatca cataccagga gatgagtaac
     2581 atggtggtaa agagcgccca cagcagggtt aatgcaatgc tgatactgcc gaaatcgccg
     2641 cgcacgcctt cgcttagcgt atggacgcgc accagcacac cgtcgaaatg gtccagacgt
     2701 tcccaactaa catagcgact gtccatacga atgccgcccc gcgtgtcatg ttccattgcc
     2761 tgcgccagca aggccagttc acgaggatca aaaatattcc ctgttggatg atcaggattg
     2821 gaagaggtca aaaatctcag cttactgtca tagagctgat actcaccatc gaggttttta
     2881 tcgatggcgt ttcttaaaaa ttgctgcatg gtacgcacgg gaatactcat ccccagcacg
     2941 ccataccagt agttattact gtctaccgga acactgacgg taacctgcgg ttcagtattg
     3001 ctggcgtgtt ccggttgcga agtaaaccag cgtaccgcgc ggtgacgatt ttctcgttgc
     3061 gaatggccga taaaccaggg ttgggtgaca tagccgtaat aacgcgttgg tacattgcgc
     3121 gtaaacaagg tcggctgcgt cgaaacgtaa aatccggcac gcgagacata catcgcctgt
     3181 tcaaccatcg acgaggtgtt gtgcgccagt cgcagcaagt aaccaacttc cagtgcagcg
     3241 gtaatttcat tgtcgaggct ttcattttcg cgagacagga gattcccctc gctgactaat
     3301 gcatccgata cgccattgac tgacagggtg cgtcgtcggt tgagttcaat ttgccaggcg
     3361 tgctcgtcgc gatgctgttc gaactcggtt acagcattac gcagtgaagt gaaatccagt
     3421 ggcgctacga gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc gacgttatac
     3481 tgcaaatgct tatcgagcgc gttggctacg ttttccagat gattacgctg gctggagata
     3541 taggcatctt ccagcaccac tacttcacgc caggtgagca aggtggaaaa aagcaatacc
     3601 acgataaagc agagattaac gacatgacca ggccccaggc ggcgtgcgag ttttttcaac
     3661 cagctctggt tttccatttt tgtctcgtgc tgcaccctga cgactcccaa cctgcttttt
     3721 tatgattctg gcataacctg gcgatagcgt accgcaaaac agcgataatt ttataatggt
     3781 ggcatgataa gacgcgacag acatcgatag ttttataacg taggcatgat aagacgcgat
     3841 agcgtcgcat caggcaattc agtgcagaca ctggcacacc cgaccggatg cgacgtgagc
     3901 gccacatccg gtcgggtgtt aatgataccc tttcaaactt cttccgcagg acgactcccc
     3961 tcccctggaa caagctcggt ttcatgaaaa gcttcccgct tgaccccgta accgtcatac
     4021 caacgacact caaccatacc gctggagtat ccagtgacaa tcatccgcgg gccgccctct
     4081 ttaaccgtaa cttcctcact aaccataaag ctcatactcg cctccttttt tcgagtgaaa
     4141 cttgttcacc ttagttgaag atggcgaatt ttgcataata gcctgtgcga gtattaatgt
     4201 gcctgaccgc gcattttcgc tacggctttc actcccagca ccacgatgcc gccgatgata
     4261 aatccaagaa tcagatttaa aacagtcggt aatatcatcg ccaccactgc actttgctgc
     4321 ccggcgaaat gttcaatggc gtgatgcagc ggcgcaatac catgtaccac aatcccgccg
     4381 ccgacgagga acatcgccag cgtgccgaca atcgataacg ctttcatcag ccagggcgcg
     4441 ataatcaata atcctttacc taatgcctgc atcagcgcgc tggatttttc cgccagccaa
     4501 taccccaggt catcaatctt aacgataacc cctaccagac cgtagacgcc cacggtcact
     4561 accagcgcga tgcctgacag taccagcacc tgattaagca acggcgcttc ggccacaatc
     4621 cccagcgtga tggcgacgat ttcggcagac aatataaaat cggtacgaat cgctcctttt
     4681 attttgtcct tttcaaactt cagcggatcc tgcgccgcca gcttctccag acgctgctgg
     4741 ctctgcgccg gatcttcttt atgtttacgc gcctccagca tatgcagcac tttctctacc
     4801 ccttcaaagc agagaaacgc gccaccaatc atcaacagag gcgtaatcgc ccacgggata
     4861 aacgcactga taatcagcgc cagtggcacc agaatcactt tattgatcag cgatcctttc
     4921 gccacgcccc agaccacggg aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca
     4981 ttgagcgata agtcatcccc taatacaccg gcggttttct tcgccgccag tttgcccatc
     5041 acggagatat cgtccagcag tgtggcgata tcatcgagca acgtcagtaa actactcccg
     5101 gccaaaagca ttccctctca tttttattgt tgaagggtgt aagtatgaag caaaaacggc
     5161 agctctgata cttgcttcac aggtcaacat ttttttaaca atcaagtgca attatatctc
     5221 tcgccagcag aatgattttc acgtttacta tgaaccacct ttttatttcc accgtgaggg
     5281 attatgcgtt tccgccagtt attaccgctt tttggcgcgc tgtttgcgtt gtatatcatt
     5341 tggggctcaa cctattttgt cattcgaatt ggcgtggaaa gctggcctcc gttaatgatg
     5401 gcaggcgttc gattcctggc agccggtatt ttattgctgg catttttgct actgcgcgga
     5461 cacaagctcc ccccgctacg tccgttgctc aatgccgcgc tgattggcct gttattgctg
     5521 gctgtcggta atggcatggt gacggttgcc gaacatcaaa atgttccttc cggcatcgcc
     5581 gccgtagtgg ttgcaaccgt gccccttttt accctgtgct tcagccgcct gtttggcatt
     5641 aaaacgcgca aactggaatg ggtgggtatt gccattgggc ttgccggaat catcatgctc
     5701 aatagcggtg gaaatttaag cggcaatccg tggggcgcga ttctgatttt aatcggctcg
     5761 attagctggg cgtttggctc ggtttatggc tcgcgcatta ccttacctgt agggatgatg
     5821 gcgggtgcga ttgagatgct ggcggctggc gtggtgttaa tgattgcgtc gatgattgcg
     5881 ggtgaaaaac tgacggcgct cccttccctt tcaggcttcc ttgcggtcgg ctatctggcg
     5941 ctgtttggtt cgattatcgc cattaacgct tatatgtatt taatccgcaa cgtcagtccg
     6001 gctctcgcca ccagctacgc ctacgttaac ccagtggtcg cggtcttgct gggtacgggg
     6061 ctgggtggcg aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat tgttttcgcg
     6121 gtggtactgg tcacgttggg aaaatatctc ttcccggcca aacccgttgt cgcgccagtt
     6181 attccggatg catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc
     6241 ttcgccgcag atccactctt ccagacgctc ggtaagcgcc gcatccgtca gcttctcgcg
     6301 cccgcgtaac gcacattccc agacaatcaa tacgcgccaa ccgagttcct gcaaatggct
     6361 gatatcacga cgatcgcgct cgacattttt acctatcttc tccagccaaa aatctgttcg
     6421 cgtggcgggg actttaaaca ggtagcaatg gtgatggtgc cagaagcaac cgtgggcaaa
     6481 tatcacacag cgaaattcat caaggacaaa atccgggcgt ccgggcagac tggcgtcctg
     6541 aacgcgaaac gccaggccct gcccagttaa caaactggcg aggcgcttct caatcgccgt
     6601 atcacgcgtg gcaatcgcgc gcatattttt gctgcgggtg gccttatcgt gaacgtcgac
     6661 catgttgtgc ctcttgctga cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt
     6721 ttgccaccgc ggcaaagacc ggcacgacca ccgagttacc gaactggcga taagcctgag
     6781 tgtccgaaac cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg
     6841 cacattcccg aggcgttaac cgacgtgggc gatgttgctg attcagcgga tcgtcaaagt
     6901 ctttctcacc cgttgccata tcccagccgc gatcgattaa aatttccgcg ccatctttgt
     6961 aataacgcgc agacagcgta cgcgtgacgc tttgcggatt gttcggataa accattccat
     7021 aaccgaagcc gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg tacttccaca
     7081 gtaccggcgt caggatatat ttcgcctcga ccatcgggtc cagcagctgc gccagcgtca
     7141 ctcgctgtgc agggaaacat ttgctgatat cacgcagagt aaaatcggct ttcagattca
     7201 gatcgcgacg aaaacccacc agcacgatgc gttcacggtg ctgcggcaga aaatgtttgc
     7261 cgtcgatgat tttcggatcg tccggcccgt tatcttctgc atcagccacg tcatagccca
     7321 gttcgtccag cgtctgcatg atgatgcgga acgttttacc ctggtcgtga cttttcaggt
     7381 ttttgacgtt ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg cgcaccacat
     7441 caaagaacag cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct
     7501 ttttcgatac gccagccagc gaaaatggct gacaagggaa accggccagt aaaacatcgt
     7561 gttcaggaat gtgttgacga atatgttccg ccgccgcctc atcactcacg ccttcttgat
     7621 ggctgagggt gatgtcgcgg atatcttcat taaaatgatg cgtcgccggg tcgcaataat
     7681 ggttggcttt ataagtgcgt accgcatgtt tgttccattc gctggtaaac acgcactgtc
     7741 caccaatcga ttcaaaaccg cgacggatgc cgccaattcc ggcaaataga tcgataaagc
     7801 gaaacgcata atgcggatga tgtgccggtg gtttgggtaa caacgtttgc agatgggcga
     7861 actctttctc acttaaacgg tgccatgccg agtcattcgc cagcgcacgt tttaaaattg
     7921 ccgcgctcca gtgattctca cccacaccat taagctgcgc caccagcgtt ttaacatcat
     7981 aaatttgcag cagtttctcc agcattgcct gtgcggcatt cccggtgctg tatgaatcgg
     8041 ttactgatat attttcctgc atagatttaa ccggccatct gagatggccg acaggttaac
     8101 ataattactg ccgtttaggg ccacgcgctc agccagcagg tgaaaacgca tctatcacct
     8161 tgtgatcgat accttcattc tcccccgcca gttcggcacc gagcttcgcc ataaactcga
     8221 ctaaaaattg cgcgttgtgc tgtgccagct gcttgcctct tgcggtttgc atggtttgcg
     8281 gtaatttcag taactttgtc tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc
     8341 gatgctgtgc gaacggatct tcgccatcaa acagcgccaa gcccaatgcc cctgaaacgg
     8401 caaatacgcg cgccaggcca atcgccccca gggcttccag ccgatccgca tcctggacga
     8461 ttttagcttc cgttgttaag ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat
     8521 gacaaacggc ctcaattttc tccgccgaaa attgcacgaa ctcttcggta atgctgccaa
     8581 cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc atttccatca
     8641 gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac
     8701 atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc acttacaccg
     8761 cctctcagcc cggtaagcac cagaaaatca ttgatatggc catgaatggc gtcggatgtc
     8821 gcgccagtg
//
LOCUS       DAFAVF010000166         8585 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFAVF010000166 DAFAVF010000000
VERSION     DAFAVF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.168"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(211..474)
                     /gene="rpsT"
                     /locus_tag="LGK42_003808"
     CDS             complement(211..474)
                     /gene="rpsT"
                     /locus_tag="LGK42_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK2660000.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            577..795
                     /locus_tag="LGK42_003809"
     CDS             577..795
                     /locus_tag="LGK42_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBK2660001.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            803..1744
                     /gene="ribF"
                     /locus_tag="LGK42_003810"
     CDS             803..1744
                     /gene="ribF"
                     /locus_tag="LGK42_003810"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBK2660002.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1787..4603
                     /gene="ileS"
                     /locus_tag="LGK42_003811"
     CDS             1787..4603
                     /gene="ileS"
                     /locus_tag="LGK42_003811"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK2660003.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4603..5097
                     /gene="lspA"
                     /locus_tag="LGK42_003812"
     CDS             4603..5097
                     /gene="lspA"
                     /locus_tag="LGK42_003812"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK2660004.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5185..5634
                     /gene="fkpB"
                     /locus_tag="LGK42_003813"
     CDS             5185..5634
                     /gene="fkpB"
                     /locus_tag="LGK42_003813"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK2660005.1"
                     /translation="MSESVQSNSAVLVHFTLKLEDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5636..6586
                     /gene="ispH"
                     /locus_tag="LGK42_003814"
                     /gene_synonym="lytB"
     CDS             5636..6586
                     /gene="ispH"
                     /locus_tag="LGK42_003814"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBK2660006.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAITLEGREENIVFEVPKELRVDIREVD"
     gene            6652..7566
                     /gene="rihC"
                     /locus_tag="LGK42_003815"
     CDS             6652..7566
                     /gene="rihC"
                     /locus_tag="LGK42_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBK2660007.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTLDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7733..8554
                     /gene="dapB"
                     /locus_tag="LGK42_003816"
     CDS             7733..8554
                     /gene="dapB"
                     /locus_tag="LGK42_003816"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK2660008.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSSKESGLFDMRDVLDLNSL"
BASE COUNT         1956 a         2139 c         2451 g         2039 t
ORIGIN      
        1 gtagatattc ttgttcagaa tgtatcagcc gatggttcta cgattcttaa gccacgaaga
       61 gttcagaaag tgtaacggca tgtctgcaaa agccagcatc ttttgactat ctggcaaccg
      121 gtagtgtgtt ctctcacgca tcataaaagc agcaggcata aaaaaacccg cttgcgcggg
      181 ctttttcaca aagcttcagc aaattggcga ttaagccagt ttgttgatct gtgcagtcag
      241 gttagcctta tgacgtgcag ctttgttttt gtggatcaga cctttagcag cctgacggtc
      301 cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat
      361 agctgcgtat actttcttga tgaaagtacg catcatagag cgacggcttg cgttgtgctt
      421 acgagccttt tcagactgaa tggcgcgctt cttagctgat ttgatattag ccaaggtcca
      481 actcccaaat gtgttctata tggacaattc aaaggccgag gaatatgccc ttttagcctt
      541 cttttgtcaa tggatttgtg caaataagcg ccgttaatgt gccggcactc gttacgtagt
      601 gatggcgcag gattctacca gcttgcgggg tgtgaataca gcttttccgc gataaaaatt
      661 gcagcaggcg gtcactttct tcccgtgatt tgcgccatgg caatgaaaag ccacttcttt
      721 ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat actcggacga
      781 ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc tcagccaggc
      841 cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc gcggtcatcg
      901 cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat
      961 gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg cacggctgac
     1021 ccggctgcgg gaaaaactgc gttaccttgc agagtgtggc gttgattacg tgctgtgcgt
     1081 gcgtttcgac aggcgtttcg cggcgttaac cgcgcaaaat ttcatcagcg atcttctggt
     1141 gaagcatttg cgcgtaaaat ttcttgccgt aggtgatgat ttccgctttg gcgctggtcg
     1201 tgaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg atatcaccag
     1261 tacgcaaact ttttgcgaag gtggcgtgcg tatcagcagc accgctgtgc gtcaggcgct
     1321 ggcggatgac aatctggctc tggcagaaag tttactgggg cacccgtttg ccatctccgg
     1381 gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg cgaatgtacc
     1441 gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc tgggcctcgg
     1501 tgaaaagccg ttacccggcg tggcaaacat tggaacacgc ccaacggttg ccggtattcg
     1561 ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc gccatataca
     1621 agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcgtcgctgg acgaactgaa
     1681 agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa caaaaccggc
     1741 ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga gtgactataa
     1801 atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg ccaagcgcga
     1861 accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcatcc gtgcggctaa
     1921 aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg gcagcattca
     1981 tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca aagggctttc
     2041 cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga tcgagctgaa
     2101 agtagagcaa gaatacggta agccgggtga gaaattcacc gccgccgagt tccgcgccaa
     2161 gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta tccgtctggg
     2221 cgtgctgggc gactggtcgc acccgtacct gaccatggac ttcaaaactg aagccaacat
     2281 catccgcgcg ctgggcaaaa tcatcggcaa cggtcacctg cacaaaggcg cgaagccagt
     2341 tcactggtgc gttgactgcc gttctgcgct ggcggaagcg gaagttgagt attacgacaa
     2401 aacttctccg tccatcgacg ttgcttttca ggcggtcgat caggatgcac tgaaagcaaa
     2461 atttgccgta agcaacgtta acggcccaat ctcgctggtg atctggacca ctacgccgtg
     2521 gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg cgctggtgca
     2581 gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa tgcagcgtat
     2641 cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcggagcttg agttgctgcg
     2701 ctttacccat ccgtttatgg gcttcgacgt tccggcaatc ctcggcgatc acgttaccct
     2761 ggatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg actatgtgat
     2821 cggtcagaaa tacggcctgg aaaccgctaa cccggttggc ccggacggca cttatctgcc
     2881 gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca tcgtcgttgc
     2941 gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca gctatccgtg
     3001 ctgctggcgt cacaaaacgc cgatcatctt ccgcgcgacg ccgcagtggt tcgtcagcat
     3061 ggatcagaaa ggtctgcgtg cgcagtcact gaaagagatc aaaggcgtgc agtggatccc
     3121 ggactggggc caggcgcgta tcgagtcgat ggttgctaac cgtcctgact ggtgtatctc
     3181 ccgtcagcgc acctggggcg taccgatgtc actgttcgtg cacaaagaca cggaagagct
     3241 gcatccgcgt actctcgaac taatggaaga agtggcaaaa cgcgttgaag ttgacggcat
     3301 ccaggcgtgg tgggatcttg atgcgaaaga gatcctcggc gatgaagctg atcagtacgt
     3361 gaaagtgccg gacacattgg atgtatggtt tgactccgga tctacccact cttctgttgt
     3421 tgacgtgcgt ccggaatttg ccggtcacgc agcggacatg tatctggaag gttctgacca
     3481 gcaccgtggt tggttcatgt cttccctgat gatctccacc gcgatgaaag gcaaagcgcc
     3541 gtatcgtcag gtactgaccc acggctttac cgtggatggt cagggtcgca agatgtctaa
     3601 atccatcggc aacaccgttt cgccgcagga tgtgatgaac aaactgggcg cggatattct
     3661 gcgtctgtgg gtggcatcaa ctgactacac cggtgaaatg gccgtttctg atgagatcct
     3721 gaaacgtgct gccgacagct atcgtcgtat ccgtaacacc gcgcgcttcc tgctggcaaa
     3781 cctgaacggt tttgatccgg caaaagatat ggtgaaaccg gaagagatgg tggtactgga
     3841 tcgctgggcc gtaggttgtg cgaaagcggc acaggaagac atcctcaagg cgtacgaagc
     3901 atacgatttc cacgaagtgg tacagcgtct gatgcgcttc tgctccgttg agatgggttc
     3961 cttctacctc gacatcatca aagaccgtca gtacaccgcc aaagcggaca gtgtggcgcg
     4021 tcgtagctgc cagactgcgc tgtatcacat cgcagaagcg ctggtgcgct ggatggcacc
     4081 aatcctctcc ttcaccgctg atgaagtgtg gggctacctg ccgggcgaac gtgaaaaata
     4141 cgtctttacc ggtgagtggt acgaaggcct gtttggtctg gcagacagtg aagcgatgaa
     4201 cgatgcgttc tgggacgagc tgttgaaagt gcgtggcgaa gtgaacaaag tcattgagca
     4261 agcgcgtgcc gacaagaaag tgggtggctc gctggaagcg gcagtaacct tgtatgcaga
     4321 accggaactg tcggcgaaac tgaccgcgct gggcgatgaa ttacgatttg tcctgttgac
     4381 ctccggcgct accgttgcag actataacga cgcacctgct gatgctcagc agagcgaagt
     4441 actcaaaggg ctgaaagtcg cgttgagtaa agccgaaggt gagaagtgcc cacgctgctg
     4501 gcactacacc caggatgtcg gcaaggtggc ggaacacgca gaaatctgcg gccgctgtgt
     4561 cagcaacgtc gccggtgacg gtgaaaaacg taagtttgcc tgatgagtca atcgatctgt
     4621 tcaacagggc tacgctggct gtggctggtg gtagtcgtgc tgattatcga tctgggcagc
     4681 aaatacctga tcctccagaa ctttgctctg ggggatacgg tcccgctgtt cccgtcgctt
     4741 aatctgcatt atgcgcgtaa ctatggcgcg gcgtttagtt tccttgccga tagcggcggc
     4801 tggcagcgtt ggttctttgc cggtattgcg attggtatta gcgtgatcct ggcagtgatg
     4861 atgtatcgct cgaaggccac gcagaagcta aacaatatcg cttatgcgct gattattggc
     4921 ggcgcgctgg gcaacctgtt cgaccgcctg tggcacggct tcgttgtcga tatgatcgac
     4981 ttctacgtcg gcgactggca cttcgccacc ttcaaccttg ccgatactgc catttgtgtc
     5041 ggtgcggcac tgattgtgct ggaaggtttt ttgccttcta aagcgaaaaa acaataataa
     5101 accctgccgg ataaggccta cagattgctg cgaaatcgta ggccgcatcc ggcaaaaatc
     5161 ctcaaaatat aagagcaaac ctgcatgtct gaatctgtac agagcaatag cgccgtcctg
     5221 gtgcacttca cgctaaaact cgaggatggc accaccgccg agtctacccg caacaacggt
     5281 aaaccggcgc tgttccgcct gggtgatgct tctctttctg aagggctgga gcaacacctg
     5341 ttggggctga aagtgggcga taaaaccacc ttctcgttgg agccagatgc ggcgtttggc
     5401 gtgccgtcac cggacctgat tcagtacttc tcccgccgtg aatttatgga tgcaggcgag
     5461 ccagaaattg gcgcaatcat gctttttacc gcaatggatg gcagtgagat gcctggcgtg
     5521 atccgcgaaa ttaacggcga ctccattacc gttgatttca accatccgct ggccgggcag
     5581 accgttcatt ttgatattga agtgctggaa atcgatccgg cactggaggc gtaacatgca
     5641 gatcctgttg gccaacccgc gtggtttttg tgccggggta gaccgcgcta tcagcattgt
     5701 tgaaaacgcg ctggccattt acggcgcacc gatatatgtc cgtcacgaag tggtacataa
     5761 ccgctatgtg gtcgatagct tgcgtgagcg tggggctatc tttattgagc agattagcga
     5821 agtaccggac ggcgcgatcc tgattttctc cgcacacggt gtttctcagg cggtacgtaa
     5881 cgaagcaaaa agtcgcgatt tgacggtatt tgacgcgacc tgtccgctgg tgaccaaagt
     5941 gcatatggaa gtcgcccgcg ctagtcgccg tggcgaagaa tctattctca tcggtcacgc
     6001 cgggcacccg gaagtggaag gcacgatggg tcagtacagc aacccggaag ggggaatgta
     6061 tctggttgaa tcaccagacg atgtgtggaa actgacggtc aaaaacgaag agaaactctc
     6121 ctttatgacc cagaccacgc tgtcggtgga tgacacgtct gatgtgatcg acgcgctgcg
     6181 taaacgcttc ccgaaaattg tcggtccgcg caaagatgac atctgctacg ccacgactaa
     6241 ccgacaggaa gcggtacgtg ccctggccga acaggcggaa gttgtgctgg tggtcggttc
     6301 gaaaaactcc tctaactcca accgtctggc ggagctggcc cagcgtatgg gcaaaagcgc
     6361 gtttttgatt gatgatgcga aagatatcca ggaagagtgg gtgaaagagg ttaaatgcgt
     6421 cggcgtgact gcgggcgcat cggctccgga tattctggtg cagaatgtgg tggcacgttt
     6481 gcaacagctg ggcggtggtg aagccattac gctggaagga cgtgaagaaa acattgtttt
     6541 cgaagtgccg aaagagctgc gtgtcgatat tcgtgaagtc gattaagtca ttagcagcct
     6601 aagttatgcg aaaatgccgg tcttgttacc ggcatttttt atggagaaaa catgcgttta
     6661 cctatcttcc tcgatactga ccccggcatt gacgatgccg tcgccattgc cgccgcgatt
     6721 tttgcacccg aactcgacct gcaactgatg accaccgtcg cgggtaatgt ctcggttgag
     6781 aaaactaccc gcaatgccct gcaactgctg catttctgga atgcggagat tccgctcgcc
     6841 caaggggccg ctgtgccgct ggtacgcgca ccgcgtgatg cggcatctgt gcacggcgaa
     6901 tcgggaatgg ctggctacga ctttgttgag cacaaccgaa agccgctcgg gataccggcg
     6961 tttctggcga ttcgggatgc cctgatgcgt gcaccagagc ctgttaccct ggtggccatt
     7021 ggcccgttaa ccaatattgc gctgttactt tcacaatgcc cggaatgcaa gccgtatatt
     7081 cgccgtctgg tgatcatggg tggttctgcc ggacgcggca actgtacgcc aaacgccgag
     7141 tttaatatcg ctgccgatcc agaagcggct gcctgtgtct tccgtagcgg aatcgaaatc
     7201 gtcatgtgcg gtctggatgt caccaatcag gcaatattaa cccttgacta tctcgctact
     7261 ttgccggagt tgaatcgcac cggaaaaatg cttcatgcac tgtttagcca ctaccgtagc
     7321 ggcagtatgc aaagcggtct gcgaatgcac gatctctgcg ccatcgcctg gctggtgcgc
     7381 ccggacttat tcaccctcaa gccgtgtttt gtcgcggtag aaactcaagg cgaatttacc
     7441 tcaggcacga cggtggttga tatcgacggt tgcctgggca aaccagccaa tgtaaaggtg
     7501 gcattggatc tggatgtgaa aggcttccag cagtgggtgg ctgaggtgct ggctctggcg
     7561 tcgtaacctg tcacatgtta tcggcatgca gtcattcatc gactcatgcc tttcactgat
     7621 atccctccct gtttatcatt aatttctaat tatcagcgtt tttggctggc ggcgtagcga
     7681 tgcgctggtt actctgaaaa cggtctatgc aaattaacaa aagagaatag ctatgcatga
     7741 tgcaaacatc cgcgttgcca tcgcgggagc cggggggcgt atgggccgcc agttgattca
     7801 ggcggcgctg gcattagaag gcgtgcagct gggcgcggcg ctggagcgcg aaggatcttc
     7861 tttactgggc agcgacgccg gtgagctggc tggtgcaggg aaaacaggcg ttaccgtgca
     7921 aagcagcctc gatgcggtaa aagatgattt tgatgtgttt atcgatttta cccgtccgga
     7981 aggtacgctg aaccatctcg ctttttgtcg ccagcatggc aaagggatgg tgatcggcac
     8041 tacggggttt gacgaagccg gtaaacaggc gattcgtgac gccgccgccg atattgcgat
     8101 tgtctttgcc gccaatttta gcgttggcgt taacgtcatg cttaagctgc tggagaaagc
     8161 agccaaagtg atgggtgact acaccgatat cgaaattatt gaagcacatc atagacataa
     8221 agttgatgcg ccgtcaggca ccgcactggc aatgggagag gcgatcgccc acgcccttga
     8281 taaagatctg aaagattgcg cggtctacag tcgtgaaggc cacaccggtg aacgtgtgcc
     8341 tggcaccatt ggttttgcca ccgtgcgtgc aggtgacatc gttggcgaac ataccgcgat
     8401 gtttgccgat attggcgagc gtctggagat cacccataag gcgtccagcc gcatgacatt
     8461 tgctaacggc gcggtaagat cggctttgtg gttgagtagt aaggaaagtg gtctttttga
     8521 tatgcgagat gtgcttgatc tcaatagttt gtaaccacaa aatatttgtt atggtgcaag
     8581 aataa
//
LOCUS       DAFAVF010000167         8496 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFAVF010000167 DAFAVF010000000
VERSION     DAFAVF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.169"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            197..913
                     /gene="nanC"
                     /locus_tag="LGK42_003817"
     CDS             197..913
                     /gene="nanC"
                     /locus_tag="LGK42_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBK2660009.1"
                     /translation="MKKAKILSSVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            933..2039
                     /gene="nanM"
                     /locus_tag="LGK42_003818"
     CDS             933..2039
                     /gene="nanM"
                     /locus_tag="LGK42_003818"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBK2660010.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSVDIHLWYNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVMDSVLISVKDNKVTVQN"
     gene            2104..3084
                     /locus_tag="LGK42_003819"
     CDS             2104..3084
                     /locus_tag="LGK42_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBK2660011.1"
                     /translation="MNAIISPDYYYILTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(3092..3742)
                     /locus_tag="LGK42_003820"
     CDS             complement(3092..3742)
                     /locus_tag="LGK42_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK2660012.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            complement(4787..4939)
                     /locus_tag="LGK42_003821"
     CDS             complement(4787..4939)
                     /locus_tag="LGK42_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660013.1"
                     /translation="MSDITAYRPEVVNENTDVICSTSIRRILAVRKSSLLQIDTIIRH
                     QVSDIS"
     gene            complement(5024..5221)
                     /locus_tag="LGK42_003822"
     CDS             complement(5024..5221)
                     /locus_tag="LGK42_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660014.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5233..5721)
                     /locus_tag="LGK42_003823"
     CDS             complement(5233..5721)
                     /locus_tag="LGK42_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660015.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(5718..6092)
                     /locus_tag="LGK42_003824"
     CDS             complement(5718..6092)
                     /locus_tag="LGK42_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBK2660016.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(6182..6550)
                     /locus_tag="LGK42_003825"
     CDS             complement(6182..6550)
                     /locus_tag="LGK42_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBK2660017.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWRLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(6713..6934)
                     /locus_tag="LGK42_003826"
     CDS             complement(6713..6934)
                     /locus_tag="LGK42_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBK2660018.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6997..7473)
                     /locus_tag="LGK42_003827"
     CDS             complement(6997..7473)
                     /locus_tag="LGK42_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBK2660019.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(7489..7974)
                     /locus_tag="LGK42_003828"
     CDS             complement(7489..7974)
                     /locus_tag="LGK42_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2660020.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8029..>8496)
                     /locus_tag="LGK42_003829"
     CDS             complement(8029..>8496)
                     /locus_tag="LGK42_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBK2660021.1"
                     /translation="YFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDR
                     IEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSA
                     YQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         2264 a         1973 c         2131 g         2128 t
ORIGIN      
        1 tttaatctgg ccctctgttt atttaaataa tgtggaaaga gatttttcac aggagatatt
       61 atacaaaaaa tataaaatac agctaccggt tgccaaagac actataagcc tggcaaaaaa
      121 atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt ctgtatccta
      181 caaatgagtg aaatttatga aaaaggctaa aatactttct agcgtattat tactgtgctt
      241 ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat atcgtagtgg
      301 aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg gatggtgggc
      361 aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa atgccgcact
      421 caatgatgtt caggttgaag ttaattacgc gattaaactt gatgatcaat ggacggtgcg
      481 cccgggaatg ttaacgcatt ttagcagcaa tggcacacgc tacggaccct acgtaaaact
      541 gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg actggaaagc
      601 ttaccgacaa caagacttat ctggtgatat gtctcgtgat aacgttcatc gttgggatgg
      661 atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga cgctatacag
      721 taaacagaac gattatcgct atgcaaacca taagaaatgg gcgacggaaa atgcatttgt
      781 tctacaatac catatgacgc ccgatattac gccatacata gaatatgact accttgaccg
      841 tcagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca ttggtgtgtc
      901 atttaaactg tagtagacag gagacagtca caatgaataa aacaataacg gcgcttacta
      961 tcataatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct gtgccattta
     1021 aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt agcgcaggta
     1081 cggcatggta caagctggat acacaggcca aagataaaag atggacagcg ttagctgcat
     1141 tccctggcgg accgagagat caagcaacct ctgcatttat tgatggcaat ctgtatgtgt
     1201 ttggcggcat tggcaaaaac agcgagggct tgactcaggt atttaatgac gtacacaaat
     1261 acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcgccgatg ggcatggcgg
     1321 gccatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt aaccagaata
     1381 tcttcaatgg ctattttgaa gatctcaacg aagctggaaa agattcaacc gctatagata
     1441 aaatcaacgc ctactatttt gacaaaaaag cagaagatta tttcttcaat aagtttctgt
     1501 tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatctccc tggtacggaa
     1561 cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat ggcgaagcca
     1621 aaccaggatt gcgaacggat gccgtatttg aacttgactt caccggtgat aatttaaaat
     1681 ggaataagct tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt gcggggataa
     1741 gcaatgattc tcttatattt gctggagggg ccggattcaa aggttcacga gaaaattacc
     1801 agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc atatagcgtt gatattcatc
     1861 tttggtataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg gcctacggag
     1921 tatcattgcc ctggaataat agtttattaa ttattggcgg tgaaactgca ggcggcaaag
     1981 cagtgatgga ttcagtttta atttctgtga aggataataa agtcacagta caaaactaac
     2041 gcttcagggc cccggtaaag gggccctgct atcaatttgt catttcaatt acgcgaaatt
     2101 tatatgaacg caataatatc gcctgattat tactatattc ttaccgttgc tggtcagtct
     2161 aatgccatgg cgtatggcga aggactgcca ttaccggaca gggaagatgc gcctcatccc
     2221 agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgtc atgtcacttt
     2281 aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca gggttatcac
     2341 catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc actgcatata
     2401 gcacggaaat tactgccctt tattcctgat aatgcagggg ttctcatcgt tccgtgttgc
     2461 cgtggcggat cggcttttac cgcgggcagc gaaggaacat attcagaacg gcacggagcc
     2521 agccatgatg cttgtcgttg gggaacggat actccgctat accaggattt agtcagcaga
     2581 acgcgagccg cactggcaaa aaatccgcat aacaaattcc tcggcgtatg ctggatgcaa
     2641 ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt taatcatatg
     2701 gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa tattactgac
     2761 gcaccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc tcatgcgtat
     2821 gaagttattt atggcaatta tcaaaataat gttttagcca atattatttt cgtcgacttc
     2881 cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga cgatttaagc
     2941 acgggatatt acggttcagc gtaccggtca ccggagaact ggacgacggc actgcgaagc
     3001 agtcatttca gcacggcagc ccgtcggggg attatttctg acaagtttgt agaagcaatt
     3061 ttgcagtttt ggcgcgaaag gtgagcgttc attaatttga tttattcgca tccatcagcg
     3121 cttttaactg ctccggcgta aacggtggtt tgcgtcggtg caacatcgca tggcaattag
     3181 ggtacacagg cagcaaatca ttaactggat ccagctggta atcctcttta atggctgaca
     3241 gcggcaccag gtgatgcaca tggataaagc cttcagcgat atcaccatag atcttcacca
     3301 gatcaacacc gcatacgttg cactggcatc catgatgttc gaccgcttta tctcgggcct
     3361 ttttatcgcg ttcatagcgg tttactgtca cctttatcgc cgcaccttca aggtatgtct
     3421 cctgcgacgg caactcatcc gggaacagaa cgtcggctat gggagccgca gcggctttat
     3481 ccagatgaat atgaaatccc tgctctatga gtagcttgac acatttagat tttattccgc
     3541 cggtgaaatc cgcaggggta aattgcgtcc ccgtcatcaa tgtcgctgcg atgccagcaa
     3601 tcgccttcga ggggtaacgt cggccatgat attcaatctc atacaaccgc gcaggctgga
     3661 actgatgagt ttctcccgca tcccataaat ggatagcttc gatgatatac cgccgcggaa
     3721 gatgatcagg gagtttattc atggagaatg tccttatacg ttctcagaac aggcagcaaa
     3781 aaaacaaatg agatcaaggt aatatatctt cacttgcagg cttgaacagc agaaaatttg
     3841 cgagcatgat cgcaattatt tcactgccag ggatatcaat agcttccagt aaatcgctac
     3901 agcaggcaat tgccaatata aaaatctggc acaagggtga acagcgcgcg ccgcataagc
     3961 cattgttatt gctatacgta ttagcgggat acctgaatgg acatccgcgc cttttcgatt
     4021 attgtagtgg aacagtctac gaggatgcag ttcgaagtga gtaagtaaag gttgctggaa
     4081 acgatcaatg ccgccataca atgatttttt aggatcaaca atgagtaata aaaactacga
     4141 aagtcaccgc aaagcgattg ttagtaaagg tataccaccc gctctattaa ataggctcac
     4201 caattcggat gttcaggtga tcaatacctt cttaacacga gtgagcaaac tggaactatc
     4261 tcaacaagag aaagactgga tcataaaaat catctctatg gtttagacaa tttcagcctt
     4321 gtagcctttc aagggttttg tgtgggtaca aacattatcg aatcggggta tagccgcagc
     4381 tgtagctgta tccacatcag taagttgatg taggttgact taggttgagc gaaaaatagc
     4441 gaaaagcctt gtgtggcgcg gatttcagtc ataaaaaaac gtccgttgat gtctattgat
     4501 gttccgatgg tgcgaaggtg cgaaggtgcg aaggccggac tcaaacatgt gacttaacct
     4561 catgatttaa aattgttaat aataaacaat atcatcttat accaacacag ataccaacac
     4621 gaaaaatcac tggttttcgg catcgaacca ggacaatcag ctcctgattg agataccgag
     4681 ctctgttact cgtcatcttc ctcgacaggc ttcattgcat caagtagtac tcgcaccatc
     4741 gcgatagaac caatccggat aaaagcatct tctccatcta tatctgttaa ctgatatcgc
     4801 tcacttgatg ccggataatt gtgtcgattt gcagcagtga actctttcgt acagccagaa
     4861 tgcggcggat gctggttgag cagataacgt ccgtattctc gttgaccact tccggacggt
     4921 aggctgtgat atcagacata atcgatattc ctttttgcag ataaatgaaa atgccagccc
     4981 tgttcaggct ggcgtcagtg gaatgaagtc tggtgagtaa gactcaccgg ttctggtgct
     5041 gaagctgcag ttggcgcagg gtacggatat cctctctggc ctgttcgata cagggcaaca
     5101 gtgctgctga atccgtttta tcctcattgt tgtcaaagat aattcccgat tcgcagtcga
     5161 tattgtcctg cagccacgca atcagaatat ccagtgcggt ttccgtggtt aatgatttca
     5221 tgttgttcct ggttaccagt tgaaagtggg taaacccggc aggcattcag cgctggcatc
     5281 cagatgaatg agactgacgc cataacgtcg ggtaagtgtg ataaccagac gacggaacgt
     5341 ttttgacagc cccaggcgtt taagccgcag aaccggatat gaccacgcat cggtacgtaa
     5401 cagatatccg ctacctgtga aatgaatcca ttctgattca ccaacgtcac tggtctgatg
     5461 cgacagcgag tacagccagg cgttatcctt ttccgtgata tgtgcggtac tgcagcgtat
     5521 gccggcaagg gttgcaaacg gtggtagtgt gcaggttgac tgttggtcgg attcctccac
     5581 cacgcggagt gaataaccgt tttcagcgac cttgttaatc agttcagtga gattaacacc
     5641 atcgacgtca acgacaattc gccccatgtt cagtgcctgc acattaattc tgtcggcttc
     5701 cagcatcagg gaaagtttca tcgtttctcc ggatgcttac ccagggtaat gttatttacc
     5761 gttctgtaat tgtcgcgggt catcaggccg gtcgccctgc gagcccggag gatatcaata
     5821 ctgtttatta actgagagcg gggacaagtg ctgaatcccg gctggtcggt acgcaccagc
     5881 gcgtattttt ccacgagaaa attcaccgca tcacacagtg aaatgccagc ttcaatatgc
     5941 tgctcaatca cacgttcatc ggcgaacggt gtgtcattca gtgtaaggcc gtaatgctgg
     6001 cccagtagtc gggagagcag agtctgccag atggtgacgg gagacgggca gctcgatgcc
     6061 tcccgtacgt gtgtatcggg taatgttttc atactgaagg ttttcctgat atgcagagat
     6121 gaaaaatgga aaagtggcgt ggtgaaagca ccaggccgga gcagaaggtt attccggtgg
     6181 tttacttttt cgtttcgggc gtcggataaa cagccagata aacgtaacca ccgctaccga
     6241 gagtgtcagc tttgcaggtc agaccttttg catacagcgt gacggtatgc tgatggcggg
     6301 gattcagttc accgctggtg agcatgagtt ccagttgttt catcagcagc ggaaaggcct
     6361 ggtccaggtg gtacgcatct gcatcgctga acaggcctct gataccggcg cggtcggcaa
     6421 ggtaatgcag gcggttaccc tcctgcacca gacgggcgcc gaaacagggc gtcactgtgc
     6481 agggtagtct ccaccaggga tggtcgtcat tgtcatcggg atgcgttgtc ccggaaagtg
     6541 tgtctgacac gataaaatcc tcacagaaaa tcggtgaatg atagtttaac gatgacgggt
     6601 cagccggcgc gcagaccggc ttcggtaaag gtgtattcgt tgatgagcag gacctcatcc
     6661 acagcggcgt ctgagctcag ccagtcgtac tcgttttccg ggtgacggaa catcagttca
     6721 gggtgacgct catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca
     6781 cggccagcac accgggaata tcctgcactt cacgaccggt atacgcttca gcactgccgt
     6841 gccagcggta tttgccggta cagaacggaa aaagacggga tgccggatgc tgacggtgaa
     6901 tacgcatggc ttcgccacgg gtgatgattt tcatagtggg atacctctga agacagaaga
     6961 taaaagtgaa aacaggtgtg atgtggttgt gacgggttaa agcagaccat gttcggcaaa
     7021 ggagaaaacc tggctgccac cgactatcag atggtccggc acccggatat ccaccaggcc
     7081 cagtgcctgt accagacgtt ccgtgataag gcggtctgcc ttgctgggtg tgacttcacc
     7141 ggacgggtga ttgtgtgcca gcaccacagc agcggcattg tgatacaggg cgcgtttaat
     7201 cacttcacgg ggatggactt ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc
     7261 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     7321 catgttcaga atcagccatt ctcgtgccgc acgggtggag gtgaaggcca cgccgggttc
     7381 atgaagatgg cggtccaggg ttttcagggc ccgttgaatg aggctgcgct cgccgggcgt
     7441 catctctccg ggcagaaagg aaagctgttg cattgtactt ctctccattc agtcgatgat
     7501 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     7561 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     7621 agcaatgccg gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag
     7681 tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt
     7741 cagggtgtac agattccaga taccaccgca gtagtcttca cacagacggt ccatccagcc
     7801 gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa
     7861 gctgatgcgc tgttcatcag ggacgagggt ggcaaccaac tgaggctggt cattccctga
     7921 tgcagcggtt acggaaacag aagatgtggt ggaattatgt gaaatggttt tcatgaaatg
     7981 actccttaaa ggacaaataa atgcgtggtt ttcagggtaa tggctgaatc agcgcaggct
     8041 ctccagcagg gtttccgcca tcacccacaa tgcgcggttg agcttaatgt cagtgtcaat
     8101 gctgtgaatg gcacgggtat ggatacgttt tcctttagcg ctgcgacctg aaattccgcc
     8161 tttcagcata ttctcctgaa tggtctgata agcactccac aggtccttac cgtaatcctc
     8221 ccggcgtcgt ggcgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt caccataacg
     8281 gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagaa tcagcgactg
     8341 catggcatca cgcttttcct caatccggtc aaacacgccc accacctcgt aagccccttc
     8401 aatgactctg tccaccacat ttccccggtg cggaacacgc acttccccca gagactgacc
     8461 acagacgcat ccgttctggc agacgaacct gaagta
//
LOCUS       DAFAVF010000168         8478 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFAVF010000168 DAFAVF010000000
VERSION     DAFAVF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.170"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            367..597
                     /locus_tag="LGK42_003830"
     CDS             367..597
                     /locus_tag="LGK42_003830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660022.1"
                     /translation="MLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFA
                     DDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(601..1992)
                     /gene="tcyP"
                     /locus_tag="LGK42_003831"
     CDS             complement(601..1992)
                     /gene="tcyP"
                     /locus_tag="LGK42_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBK2660023.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFATFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2125..2715)
                     /locus_tag="LGK42_003832"
     CDS             complement(2125..2715)
                     /locus_tag="LGK42_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK2660024.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2878..3546)
                     /gene="hxpB"
                     /locus_tag="LGK42_003833"
     CDS             complement(2878..3546)
                     /gene="hxpB"
                     /locus_tag="LGK42_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBK2660025.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3693..4229
                     /locus_tag="LGK42_003834"
     CDS             3693..4229
                     /locus_tag="LGK42_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBK2660026.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4270..5130)
                     /locus_tag="LGK42_003835"
     CDS             complement(4270..5130)
                     /locus_tag="LGK42_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK2660027.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5236..5526)
                     /gene="ghoS"
                     /locus_tag="LGK42_003836"
     CDS             complement(5236..5526)
                     /gene="ghoS"
                     /locus_tag="LGK42_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBK2660028.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5627..6556)
                     /gene="pfkB"
                     /locus_tag="LGK42_003837"
     CDS             complement(5627..6556)
                     /gene="pfkB"
                     /locus_tag="LGK42_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBK2660029.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6843..7601
                     /locus_tag="LGK42_003838"
     CDS             6843..7601
                     /locus_tag="LGK42_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBK2660030.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7654..7761)
                     /locus_tag="LGK42_003839"
     CDS             complement(7654..7761)
                     /locus_tag="LGK42_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660031.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7937..>8478)
                     /locus_tag="LGK42_003840"
     CDS             complement(7937..>8478)
                     /locus_tag="LGK42_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660032.1"
                     /translation="SMLYGFDEIIDIFLNALTTPIAQELLNKKMVMDILAMKTRDGEP
                     GLFAAMENNHPLCFTRFLSKVYGIAVKYKLSKINIMDLLKGATAHGTPALYIAMSKGN
                     KDVVLSYISTLSTFAKKYSFSQRQLFTLLAAKNHENMSAVHIAIHHNHYKTVETYYAA
                     INAISQSLSFSADELKTYL"
BASE COUNT         2249 a         2001 c         2009 g         2219 t
ORIGIN      
        1 gccaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta aatatagagt
       61 gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg agaattttgt
      121 tgggatggat tttacctgtg aattctgacc cgaaaaaatg acgaatttga tcaatataaa
      181 aaatagtttg gttattcatt atttaaggac tatatactat gaaaaaaatt atatacatcc
      241 tgccgggagc atttttgctg cgattcaaaa aaaaatgaga gtctcctaaa gtttccttcg
      301 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
      361 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
      421 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
      481 ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat cgccgcgatg
      541 gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc agcataagaa
      601 ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt
      661 cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg
      721 gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc
      781 catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc
      841 tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt
      901 aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc
      961 gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc
     1021 cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt
     1081 aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt
     1141 aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac
     1201 aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca ctttggtcat
     1261 tagagccaga acgccgtaag gggtcaactg catgacaagg cgaaccagtt tcatcaccca
     1321 gctttgtagg gtatcgatag cggctaagac gcgttcacct ttcggcgcat catccttcag
     1381 cagtttcaat gcagctacgc cgaggaatgt ggcaaaaatt accacgctga taattgacgt
     1441 cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg
     1501 cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt tcagacgtgc
     1561 agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag
     1621 cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact
     1681 gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa
     1741 gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa
     1801 ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag
     1861 gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt
     1921 atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa
     1981 tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag
     2041 tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat
     2101 tccagaaagg tgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa
     2161 ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt tctcgggtaa
     2221 tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac gttccagttg
     2281 gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag
     2341 gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat
     2401 tccttgtagc gcatcagccg gaatgaacca gccttccgga accttaaggt aaaaggttgc
     2461 cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc
     2521 gatcggtttt gatatccatt ttaatcgctg cccaagaaac gactttgggt gatcgatgtc
     2581 cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag
     2641 cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg
     2701 accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta
     2761 tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc
     2821 agttgaatgc aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta
     2881 accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac
     2941 aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc
     3001 tttagaagcg atcatgccat ttaccgagtc ttccagcgcc acgcaggtca gtgggtcaac
     3061 gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt
     3121 ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc
     3181 cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa
     3241 cgcaacagct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc
     3301 acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc
     3361 gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg
     3421 acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg
     3481 ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt
     3541 tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt
     3601 aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa
     3661 gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg
     3721 ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga
     3781 tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca
     3841 tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc
     3901 tgttctggta taactccccg acccctaatt ttaatggtgg cgtgaacgtt atgttctggg
     3961 tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc
     4021 gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag
     4081 gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta
     4141 ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg
     4201 tgttcttttc tctacttggg tttatttaat caaaaggccg cacggagcgg ccattatcct
     4261 caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg
     4321 accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc
     4381 aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg
     4441 ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg
     4501 gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg
     4561 caacagagaa ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc
     4621 gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg
     4681 ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga
     4741 gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc
     4801 aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc
     4861 catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac
     4921 ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag
     4981 ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc
     5041 atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc
     5101 gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca
     5161 taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta
     5221 ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat
     5281 ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc
     5341 ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc
     5401 atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat
     5461 ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact
     5521 ggccatagtt cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg
     5581 ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt
     5641 aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg
     5701 ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca
     5761 gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca
     5821 ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac
     5881 caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc
     5941 ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag
     6001 gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt
     6061 cgatgatgca gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca
     6121 gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca
     6181 gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa
     6241 aacgatactg ctcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt
     6301 tggcttctac agtagcgacg gggacatttt catccgccaa cagagaaacc aggtgttcgc
     6361 cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc
     6421 gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc
     6481 cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg
     6541 tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcc
     6601 gaaagtgaag aaattaacaa tatgatttat tagagcttta tttaaaattt tgcagataaa
     6661 tatatataaa taaaaatctg ttttcgccac atgcttattc aaaaaatgaa ataagaatac
     6721 attgctaagt tattcgcgcc tttttataat tcgttacctt tccagggacg ctaatctttg
     6781 atccttaaga aagtttccgg gaccgcgatg gtctcttttt ttgttgtacg gactcttgat
     6841 aaatgaagct tttgaagaca gttcctgcca tcgttatgtt ggcggggggc atgtttgcgt
     6901 cactgaatgc agctgccgat gattccgttt ttactgtcat ggacgatcct gccagcgcta
     6961 aaaaaccttt cgaaggtaat ctgaacgcag gatatcttgc acaatcaggc aatacgaaaa
     7021 gctcctcact tacggctgac accaccatga cctggtatgg acaaaccact gcctggtcgc
     7081 tgtgggggaa cgccagtaat acttcttcta atgatgagcg ttcttcagaa aaatatgcgg
     7141 cgggcgggcg tagccgtttt aatttaactg attatgacta tttatttggt caggcaagct
     7201 ggctgacaga ccgttataac ggctatcgag agcgcgatgt gttgaccgcg ggttatggtc
     7261 gtcagttttt gaatggcccg gtacacagtt tccgctttga attcggtcca ggtgtgcgtt
     7321 acgacaaata taccgacaat gccagtgaaa ctcagccgct gggctatgcc tccggtgctt
     7381 atgcatggca gttgactgac aacgctaaat ttacgcaggg tgtttccgtc tttggcgcgg
     7441 aagacacaac gctgaactcc gaaagcgcat taaatgttgc tattaatgaa cattttggac
     7501 tgaaagtggc ttacaacgtc acatggaact ctgaacctcc agagtctgca ccggaacata
     7561 ccgatcgtcg gacaacctta tcgctgggct acagcatgta aaattatctg ccggaccgtg
     7621 ttatgcggtc tggctgtctt cgtttgtcac tgctcagccc caaatcattg ctgcccaacg
     7681 tagcaacaac atagcgccac agattgccag tagcaccacc accatcttcc agtgtttttt
     7741 atcaaagcgt ttagtcggca taccagtgtc ctgcatatct cttgtgcgtg ataacggagt
     7801 gtaacaaaag ggacaggcat caagggattt tttgttatta cagccgtcgg gtggcgttgt
     7861 acacctgatc actgtttctg cgttttatga aaaaaataaa attatgtatc gatatacaaa
     7921 tatcataaat agctggttat aaatacgttt ttagttcatc agcactaaaa ctcaggcttt
     7981 ggctgattgc attaatagca gcataatatg tttctacagt tttataatga ttatgatgaa
     8041 tggctatatg aacagctgac atgttttcat gatttttagc ggccaacagt gtgaataatt
     8101 gacgttgact aaaagaatat ttttttgcaa aagtactcag tgttgatata taagataaca
     8161 cgacgtcttt attacccttg ctcatggcga tgtataaagc aggggttcca tgtgctgtcg
     8221 cgccttttaa taagtccatg atgttgattt tgctgagttt atatttaacg gcgatcccat
     8281 aaactttaga gaggaaccgt gtgaaacaca aagggtgatt attttccatt gcagcaaata
     8341 accctggttc accatcacgt gttttcatgg ctaaaatatc catcaccatt tttttgttta
     8401 aaagttcttg tgctattgga gtggttaatg cattcagaaa gatatcgatt atttcatcga
     8461 atccatacaa catggaga
//
LOCUS       DAFAVF010000169         8412 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFAVF010000169 DAFAVF010000000
VERSION     DAFAVF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.171"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(60..1652)
                     /locus_tag="LGK42_003841"
     CDS             complement(60..1652)
                     /locus_tag="LGK42_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBK2660033.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="LGK42_003842"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="LGK42_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2660034.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="LGK42_003843"
     CDS             complement(2850..3968)
                     /locus_tag="LGK42_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBK2660035.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTAIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="LGK42_003844"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="LGK42_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBK2660036.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="LGK42_003845"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="LGK42_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBK2660037.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="LGK42_003846"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="LGK42_003846"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBK2660038.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="LGK42_003847"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="LGK42_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBK2660039.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="LGK42_003848"
     CDS             complement(7218..7283)
                     /locus_tag="LGK42_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660040.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="LGK42_003849"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="LGK42_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBK2660041.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGSALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2078 a         2230 c         2014 g         2090 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaaa gcatccgccc tttttctcca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttgccgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgtacgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggcagtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta ca
//
LOCUS       DAFAVF010000170         8362 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFAVF010000170 DAFAVF010000000
VERSION     DAFAVF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.172"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(187..1077)
                     /gene="malG"
                     /locus_tag="LGK42_003850"
     CDS             complement(187..1077)
                     /gene="malG"
                     /locus_tag="LGK42_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBK2660042.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1092..2636)
                     /gene="malF"
                     /locus_tag="LGK42_003851"
     CDS             complement(1092..2636)
                     /gene="malF"
                     /locus_tag="LGK42_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBK2660043.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2790..3980)
                     /gene="malE"
                     /locus_tag="LGK42_003852"
     CDS             complement(2790..3980)
                     /gene="malE"
                     /locus_tag="LGK42_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBK2660044.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4345..5460
                     /gene="malK"
                     /locus_tag="LGK42_003853"
     CDS             4345..5460
                     /gene="malK"
                     /locus_tag="LGK42_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBK2660045.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5532..6872
                     /gene="lamB"
                     /locus_tag="LGK42_003854"
     CDS             5532..6872
                     /gene="lamB"
                     /locus_tag="LGK42_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBK2660046.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7115..8035
                     /gene="malM"
                     /locus_tag="LGK42_003855"
     CDS             7115..8035
                     /gene="malM"
                     /locus_tag="LGK42_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBK2660047.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2063 a         2180 c         2161 g         1958 t
ORIGIN      
        1 attatcgtaa gtgggaaaac aaataacgta aaaataataa gctctatgat gggaatatta
       61 accggcgaac gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg agcgccgaat
      121 aacagtcaca agttgggata acgtaagttg agggtgcagc ggcataacat tggcagaaca
      181 acatctttaa cctttcacac cacctgccgt caggccgttg accagccagc gttgagccag
      241 caagaagacg atggtgatcg gtaatgcaga catcacggca gcggcggcaa agtcacccca
      301 caggtagttt tgcgggttga ggtattgctg catccccacg gccagggtgt agctgtttac
      361 gtcacgcagt aacagcgacg cgaccggaac ttcagtaatg gcagcgataa acgacaggat
      421 gaataccacc gccagaatcg gtactgacag cggcaacagg acaaggcgga aggcctgcca
      481 cggtgtcgca ccatccagcg cagccgcttc ttccagcgaa ctgtcgatgg tttcgaaata
      541 gcctttgatg gtccagacat gcagcgcaat cccacccaga tacgcgaaaa ttacgccgcc
      601 gtgagtattc aggccaatga atggaatgta ctcacccaga cgatcaaaca acgcatacaa
      661 cgcgaccagt gaaagtactg ccgggaacat ctggaaaatc agcattcctt tcagcagcgt
      721 cgctttgcct ggaaagcgca tacgggcgaa agcgtaggcg caggtggtgg agagcgccac
      781 aatgccaatc gcggaaatcc cggcgacctt taccgagttc cacagccaca gcagtaccgg
      841 gaatggcggt ggcgtaatgc gaccatcagc ctgttcaacg ctaaaaccta acgccagttt
      901 ccagtgatcc caggagattt gctccgggat caggctaccg gtcgcgaagt ttccctgacg
      961 cagcgagata gcgacgacca tcagcagcgg gaacataatc gccgcgataa aaagtagcag
     1021 tagcaggtga gtaataaaca aacgtgcttt ttgcgatttc ggttggacca ttgccatttt
     1081 tgttatctcc cttaatcaaa cttcattcgc gtggctttca ggttcactat cgccagcgca
     1141 cccaccagca ggaagatcag cgtggcaatt gctgcagcca gaccgaagtc ctgacccccg
     1201 ccgccttcaa aagcgatacg gtaggtgtag ttaacaagca ggtcggtata accggctggc
     1261 gtggtcgtgc caagacgatc cgggccgccg ttggttaaca gctgaatcag cacgaagttg
     1321 ttaaagttaa aggcgaagct ggcgatcatc agcggcgtca gcggtttaat cagcagcggc
     1381 agcgtaatct taaagaagtt ctggaacgga cctgcgccat ccattgctga ggcttcatac
     1441 aaatcgtccg gaatcgcttt cagcaagccc atgcagagga tcatcatgta cggataaccc
     1501 agccaggtat tgacgataat cagcatcgtt cgggcagtgg tcggatcgct gaaccaggcg
     1561 ggcttcacgc caaacagcgc gctcaacatc atgttgattt caccgaagct ctggttaaac
     1621 aaccctttga aaatcaagat tgaaatgaat gatggcaccg cgtagggcag aatcagcagg
     1681 acgcgatata ccgctttgcc acgcaacgct tcccactgca ccagacacgc cagaaccatg
     1741 ccgaccgcca ccgttaaaaa gacggtgatc agtgaaaaca ccacggtcca gacgaaaatg
     1801 gcgaggaacg gtttctgaat gccttcgtcg gtaaagacgc gggtaaagtt tttccagccg
     1861 gtggtcacgg tgtaaccggg gcttagcttt tcatcccccc agttgccgtc ggcggtaatg
     1921 gactggtaaa agccaatttg gttattcgga cgatatttca cgccactctg attattcgtc
     1981 aacgtgccgt taccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg gcgcagggag
     2041 ctcatcatca ctttgttgcc atccggcaga atggcggtaa tgtcactcag cgcctgacga
     2101 ttctgggtaa tcacgcgcag attcgcgcgt tcgccttcgg gctgggcggt cgtttctttc
     2161 agttgcagtt tttgctcgcc gccaaattta aacgcgtcgg agaggtaatt tttgccggtt
     2221 tcgccgtcgc tgagcgccag ttgccactca tcgcccgccg ggtaaagacc aaagttatag
     2281 gttttgcctg cttgccagga gcgatctaac aacacttcct gcgcacgttc aaaagtcagc
     2341 tggttagtgc tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag agggaagagg
     2401 acgaataatc ccattccagc cattcccggg taaacatagc gccaggcgta ggctttacga
     2461 ttggcgaaaa tatacagccc cgctgaactc aatatcagcg tggtaatggc gaacaggtat
     2521 tccccttgtg cgtacattaa aacaacaagg taacccacca gcaggccgag cagacctagc
     2581 actgaccatt tcagcgcgtc gctttgccac caatgtttct ttttaatgac atccatgggg
     2641 ttcttcctca ttccaggacg gataaggctt tcacgcctta tccgacaaca actgcctgat
     2701 gcgacactga tgcgtcttat caggcctaca tgcgtttcag ttttgtaggc cggacaaggc
     2761 gttcacgccg catccggcat ttcacagcat tacttggtga tacgagtctg cgcgtctttc
     2821 agggcttcat cgacagtctg acgaccactg gcggcgttga tcaccgcagt acgcacggca
     2881 taccagaaag cggacatctg cgggatgttc ggcatgattt cacctttctg ggcgttttcc
     2941 atggtggcgg caatacgtgg atctttcgcc aactcttcct cgtaagactt cagcgctacg
     3001 gcacccagcg gtttgtcttt attaaccgct tccagacctt cgtcagtcag cagatagttt
     3061 tcgaggaact ctttcgccag ctctttgttc ggactggcgg cgttaatacc ggcgctcagc
     3121 acgccaacga acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa
     3181 ttcactttgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt
     3241 tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg
     3301 tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc
     3361 acgtctttaa tgtcgtactt gccgttttca tacttgaacg cataaccccc gtcagcagca
     3421 atcagcggcc aggtgaagta cggttcttgc aggttgaaca tcagcgcgct cttacctttc
     3481 gctttcagtt ctttatccag cgccgggatc tcttcccagg tttttggcgg gttcggcagc
     3541 agatctttgt tataaatcag cgataacgct tcaacagcga tcgggtaagc aatcagcttg
     3601 ccgttgtaac gtacggcatc ccaggtaaac ggatacagct tgtcctggaa cgctttgtcc
     3661 ggggtgattt cagccaacag gccagattga gcgtagccac caaaacggtc gtgcgcccag
     3721 aagataatgt cagggccatc gccagttgcc gcaacctgag ggaatttctc ttccagttta
     3781 tccggatgct caacggtgac tttaattccg gtatctttct cgaatttctt accgacttca
     3841 gcgagaccgt tatagccttt atcgccgtta atccagatta ccagtttacc ttcttcgatt
     3901 ttggcgagag ccgaggcgga aaacatcatc gtcgttaatg cggataatgc gaggatgcgt
     3961 gcacctgttt ttattttcat aatctatggt ccttgttggt gaagtgctcg tgaaaacacc
     4021 taaacggact ctagtttctt tatacggcaa cctctttcca tcctccttgc ccctacgccc
     4081 caccgttgct ttgtgtgatc tctgttacag aattggcggt aatgtggaga tgcgcacata
     4141 aaatcgccac gatttttgca agcaacatca cgaaattcct tacatgacct cggtttagtt
     4201 cacagaagcc gtgttctcat cctcccgcct cctcccccat aaaaaagcca gggggtggag
     4261 gatttaagcc atctcctgat gacgcatagt cagcccatca tgaatgttgc tgtcgatgac
     4321 aggttgttac aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt aacgaaagcc
     4381 tggggcgagg tcgtggtatc gaaagatatc aatctcgata tccatgaagg cgaattcgtg
     4441 gtgtttgtcg gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat tgccgggctt
     4501 gagacgatca ccagcggcga cctgttcatc ggtgagaaac ggatgaatga cactccgcca
     4561 gcagaacgcg gcgttggtat ggtgtttcag tcttacgcgc tctatcccca cctgtcagta
     4621 gcagaaaata tgtcatttgg cctgaaactg gcaggcgcaa aaaaagaggt gattaaccaa
     4681 cgcgttaacc aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg caaaccgaaa
     4741 gcgctctctg gcggtcagcg tcagcgtgtg gcgattggcc gtacgctggt ggccgagcca
     4801 agcgtatttt tgctcgatga accgctctcc aacctcgatg ctgcactgcg tgtgcaaatg
     4861 cgtatcgaaa tctcccgtct gcataaacgc ctgggccgca caatgattta cgtcacccac
     4921 gatcaggtcg aagcgatgac gctggccgac aaaatcgtgg tgctggacgc cggtcgcgtg
     4981 gcgcaggttg ggaaaccgct ggagctgtac cactatccgg cagaccgttt tgtcgccgga
     5041 tttatcggtt cgccaaagat gaacttcctg ccggtaaaag tgaccgccac cgcaatcgat
     5101 caagtgcagg tggagctgcc gatgccaaat cgtcagcaag tctggctgcc agttgaaagc
     5161 cgtgatgtcc aggttggagc caatatgtcg ctgggtattc gcccggaaca tctactgccg
     5221 agtgatatcg ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca actcggcaac
     5281 gaaacccaaa tccatatcca gatcccttcc attcgtcaaa acctggtgta ccgccagaac
     5341 gacgtggtgt tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc agagcgttgc
     5401 catctgttcc gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc gggcgtttaa
     5461 gcaccccaca aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa agcaatgact
     5521 caggagatag aatgatgatt actctgcgca aacttcctct ggcggttgcc gtcgcagcgg
     5581 gcgtaatgtc tgctcaggca atggctgttg atttccacgg ctatgcacgt tccggtattg
     5641 gctggacagg tagcggcggt gaacaacagt gtttccagac taccggtgct caaagtaaat
     5701 accgtcttgg caacgaatgt gaaacttatg ctgaattaaa attgggtcag gaagtgtgga
     5761 aagagggcga taagagcttc tatttcgaca ctaacgtggc ctattccgtc gcgcaacaga
     5821 atgactggga agctaccgat ccggccttcc gtgaagcaaa cgtgcagggt aaaaacctga
     5881 tcgaatggct gccaggttcc accatctggg caggtaagcg cttctaccaa cgtcatgacg
     5941 ttcatatgat cgacttctac tactgggata tttctggtcc tggtgccggt ctggaaaaca
     6001 tcgatgttgg cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa gctggtggtt
     6061 cttcctcttt tgccagcaac aatatttatg actataccaa cgaaaccgcg aacgacgttt
     6121 tcgatgtgcg tttagcgcag atggaaatca acccgggcgg cacattagaa ctgggtgtcg
     6181 actacggtcg tgccaacctg cgtgataact atcgtctggt tgatggcgca tcgaaagacg
     6241 gctggttatt cactgctgaa catactcaga gtgtcctgaa gggctttaac aagtttgttg
     6301 ttcagtacgc tactgactcg atgacctcgc agggtaaagg tctgtcgcag ggttctggcg
     6361 tcgcgtttga taacgaaaaa tttgcctaca atatcaacaa caacggtcac atgctgcgta
     6421 tcctcgacca cggtgcgatc tccatgggcg acaactggga catgatgtac gtgggtatgt
     6481 accaggatat caactgggat aacgacaacg gcaccaagtg gtggaccgtt ggtattcgcc
     6541 cgatgtacaa gtggacgcca atcatgagca ccgtgatgga aatcggctac gacaacgtcg
     6601 aatcccagcg caccggcgac aagaacaatc agtacaaaat taccctcgca caacaatggc
     6661 aggctggcga cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc tacgccaagt
     6721 gggatgagaa atggggttat gactacaacg gcgatagcaa ggttaacccg aactacggca
     6781 aagccgttcc tgctgatttc aacggcggca gcttcggtcg tggcgacagc gacgagtgga
     6841 ccttcggtgc ccagatggaa atctggtggt aatagcaaaa cctgggccgg ataaggcgtt
     6901 tacgccgcat tcgacaacca acgcctgatg cgacgcttgc gcgtcttatc aggcctacaa
     6961 cggctgtcaa atgtaggccg gataaggcgt ttacgccgca tccggcataa aaacaggttg
     7021 tcattatctg aaaggggcga aagcccctct gattatcggg tttagcgcgc tattgcctgg
     7081 ctaccgctga gctccagatt ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc
     7141 cctctgttta tcagcagggt tactggcaag cgcgcctgga atcagccttg ccgatgttaa
     7201 ctacgtaccg caaaacacca gcgacgcgcc agccattcca tctgccgcgc tgcaacaact
     7261 cacctggaca ccggtcgatc aatctaaaac ccagaccacc caactggcga ccggcggcca
     7321 acaactgaac gttcccggca tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat
     7381 tggcgaactg accctgacgc tgaccagcga agtgaacaaa caaaccagcg tttttgcgcc
     7441 gaacgtgctg attcttgatc agaacatgac cccatcagcc ttcttcccca gcagttattt
     7501 cacctaccag gaaccaggcg tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac
     7561 accggcgttg gggcagcaaa aactttatgt tctggtcttt accacggaaa aagatctcca
     7621 gcagacgacc caactgctcg acccggctaa agcctatgcc aagggcgtcg gtaactcgat
     7681 cccggatatc cccgatccgg ttgctcgtca taccaccgat ggcttactga aactgaaagt
     7741 gaaaacgaac tccagctcca gcgtgttggt aggaccttta tttggttctt ccgctccagc
     7801 tccggttacg gtaggtaaca cggcggcacc agctgtggct gcacccgctc cggcaccggt
     7861 gaagaaaagc gagccgatgc tcaacgacac ggaaagttat tttaataccg cgatcaaaaa
     7921 cgctgtcgcg aaaggtgatg ttgataaggc gttaaaactg cttgatgaag ctgaacgcct
     7981 gggatcgaca tctgcccgtt ccacctttat cagcagtgta aaaggcaagg ggtaattacg
     8041 ccccacagtg ctgattttgc aacaactggt gcgtctcctg gcgcaccttt ttttatgctt
     8101 ccttcctggg atatgagcga ttttttatag taactcactt cttcttcact aagaatatcc
     8161 attatctcaa tgccttatca gagattcttt tcctttcgcc ggtagtgtct ggacattcag
     8221 gctacttttc caggttattt tatttctgtt atgcagaggt tttatgataa gtcatatcct
     8281 aaattctggc ggcaataact ctttgatgaa acatgatgtg gtgcaaggaa ataatatagt
     8341 agatcttgat ttactacgta at
//
LOCUS       DAFAVF010000171         8314 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFAVF010000171 DAFAVF010000000
VERSION     DAFAVF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.173"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1097
                     /locus_tag="LGK42_003856"
     CDS             <1..1097
                     /locus_tag="LGK42_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBK2660048.1"
                     /translation="PALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYFSDNKSEDGDQ
                     TYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGLKFQDVGSFDY
                     GRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGLVDGLNFAVQ
                     YQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSKRTDDQNFGL
                     NGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNFEAV
                     AQYQFDFGLRPSVAYLQSKGKNLGVVAGRNYDDEDILKYVDVGATYYFNKNMSTYVDY
                     KINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1209..2264
                     /gene="apbE"
                     /locus_tag="LGK42_003857"
     CDS             1209..2264
                     /gene="apbE"
                     /locus_tag="LGK42_003857"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBK2660049.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2338..3402
                     /gene="ada"
                     /locus_tag="LGK42_003858"
     CDS             2338..3402
                     /gene="ada"
                     /locus_tag="LGK42_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBK2660050.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3402..4052
                     /gene="alkB"
                     /locus_tag="LGK42_003859"
     CDS             3402..4052
                     /gene="alkB"
                     /locus_tag="LGK42_003859"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBK2660051.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4128..5771
                     /gene="yojI"
                     /locus_tag="LGK42_003860"
     CDS             4128..5771
                     /gene="yojI"
                     /locus_tag="LGK42_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBK2660052.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            5989..7635
                     /gene="mqo"
                     /locus_tag="LGK42_003861"
     CDS             5989..7635
                     /gene="mqo"
                     /locus_tag="LGK42_003861"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBK2660053.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7784..8272)
                     /gene="eco"
                     /locus_tag="LGK42_003862"
     CDS             complement(7784..8272)
                     /gene="eco"
                     /locus_tag="LGK42_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBK2660054.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         1968 a         2105 c         2262 g         1979 t
ORIGIN      
        1 tcccagctct gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca
       61 acaaattaga tctgtacggt aaagtagacg gcctgcacta tttctctgac aacaagtcag
      121 aagacggcga ccagacctat gtacgtcttg gtttcaaagg cgaaactcag gttactgacc
      181 agctgaccgg ttacggccag tgggaatatc agatccaggg caatacctct gaagacaaca
      241 aagaaaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gttggttctt
      301 tcgactacgg tcgtaactac ggcgttgttt acgacgtaac ttcctggacc gacgtactgc
      361 cagaattcgg tggcgacact tacggctctg acaacttcat gcagcagcgt ggtaatggct
      421 acgcaaccta ccgtaacacc gacttcttcg gtctggttga cggtctgaac tttgctgttc
      481 agtaccaggg caaaaatggt agcgtaagcg gcgaaggcat gaccaacaat ggtcgtggtg
      541 ctctgcgtca gaatggcgac ggtgtcggcg gatctatcac ttatgattac gaaggcttcg
      601 gtatcggtgc tgcagtttcc agctccaaac gtactgatga tcagaacttc ggcctgaacg
      661 gttatggtga gcgttatttg ggtaacggcg accgcgctga aacctacact ggtggtctga
      721 aatacgacgc taacaacatc tacctggctg ctcagtacac ccagacctac aacgcaactc
      781 gcgtaggttc cctgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
      841 agttcgactt cggtctgcgt ccatctgtag catacctgca gtctaaaggt aaaaacctgg
      901 gtgtcgttgc tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
      961 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
     1021 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
     1081 tggtttacca gttctaatct cgattgatat cgaacaaagg gcctgcgggc ccttttttca
     1141 ttgttttcag cgtacaaact catttttttg gtgtactctt gcgaccgttc gcatgaggat
     1201 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggctgccgcg ctcttctttg
     1261 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
     1321 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
     1381 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
     1441 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
     1501 cagtaagtga agcgatggcc gaaatcgtca ctacctcgct gcgcattggc gcgaagaccg
     1561 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
     1621 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
     1681 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
     1741 atttatatat cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
     1801 tgatggagca ggaaggtatt tcacgttatc tggtgtcagt tggtggtgct ctggatagcc
     1861 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
     1921 aaaacgcggt tcaggccgtg gtggatatca acggtcatgg cattagcacc tctggcagct
     1981 accgtaacta ttacgaactg gacggcaaac gtctttctca tgttatcgat ccgcaaaccg
     2041 ggcgtcctat cgaacacaat ctggtatccg tgacggtgat tgccccgacg gcgctggaag
     2101 ccgatgcctg ggatactggc ttaatggtac tcgggccgga gaaagccaaa gaggttgttc
     2161 gccgggaagg gctggcggtc tatatgatca ccaaagaagg tgatagcttt aaaacctgga
     2221 tgtcgccgca gtttaaaagc ttccttatca gcgaaaaaaa ttaaagcgca agattgttgg
     2281 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
     2341 aaaaaagcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
     2401 aatgccgacg gcgaattcgt tttcgccgtg cgtaccacag gcatcttttg ccgtccgtct
     2461 tgccgcgcca gacatgcttt gcgggaaaac gtctccttct acgcaaatgc cagcgaggca
     2521 ctcgccgctg gctttcgccc ctgcaaacgt tgtcagccag acaaagccaa tgcccagcaa
     2581 catcggttgg ataaaattac ccacgcctgt cgactgctgg aacaggaaac gcctgtaacg
     2641 ctggaagcct tagccgacca tgtggcgatg agtccgttcc atctgcatcg gttgtttaaa
     2701 gcgactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
     2761 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
     2821 agcagcagtt actaccgcaa agccgacgaa acgctgggca tgacggctaa acaattccgt
     2881 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
     2941 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
     3001 ctaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgatg
     3061 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
     3121 ttaccgctgg acattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
     3181 ataccttgcg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
     3241 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcat aataccctgt
     3301 catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
     3361 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
     3421 atgctgaacc gtggcaagag ccactggcgg ctggagcggt aattttgcac cgttttgctt
     3481 ttaacgctgc ggagcaactg atccgcgata ttaatgacgt tgccagccag tcgccgtttc
     3541 gccagatggt cacccccggg ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
     3601 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
     3661 aaccgtggcc cgccatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
     3721 cgggctatcc ggatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
     3781 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccgatt gtttctgttt
     3841 ctcttggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
     3901 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
     3961 acggcattca accgctgaaa gcggggtttc atccactcac cggcgactgc cgctacaacc
     4021 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
     4081 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
     4141 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
     4201 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
     4261 ataccagtct gctggtattg ccggagtttc tgggattatt gctgctgttg atggcagtca
     4321 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
     4381 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
     4441 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
     4501 ttccggaact ggtgcagggg atcattctca ctatcggttc cgcggcgtat ctgtggatgc
     4561 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggtggtt
     4621 ttgtactggt ggcgcgggtg tacaaacata tggcgaccct gcgtgaaacc gaagacaagc
     4681 tgtacacgga ttttcaaacc gtactcgaag ggcgcaaaga gctgactctg aaccgggaac
     4741 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
     4801 ttattcgcgc agacaccttc catcttagcg ccgtgaactg gtcaaacatc atgatgctgg
     4861 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
     4921 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
     4981 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
     5041 cgcctttcaa agctgagttt ccgcgcccgc aggcttttcc caactggcaa acgctggagc
     5101 tgcgtaacgt gacgtttagc tatcaggata acgcgttttc cgttgggccg attaacctca
     5161 ccattaaacg tggcgagctg ctgtttctga ttggcggcaa cggtagcgga aaatcgacgc
     5221 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
     5281 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
     5341 atgtctggct gtttgatcaa ctgctggggc cggagggtaa acccgctaac ccgcaactgg
     5401 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
     5461 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
     5521 cagaagaacg cgatattatc ctgctggatg aatgggcggc ggatcaggat ccacacttcc
     5581 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
     5641 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
     5701 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
     5761 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
     5821 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
     5881 tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg
     5941 cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg
     6001 actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg
     6061 aaagcgtccg aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc
     6121 acgttgggga cctatttacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc
     6181 ctggagggtg tcgcgcagga gagttcgaac ggctggaata acgccggaac cgggcattct
     6241 gcactgatgg aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa
     6301 gcagtcgcca ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggttgag
     6361 cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc
     6421 tggggcgagg ataacgttaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg
     6481 ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta
     6541 gtgatggaag ggcgcgatcc acaacagaaa gtagcggcaa cacgcacaga aattggcact
     6601 gatgtgaact atggcgagat cacccgccag ttaattgctt ccttacagaa gaaatctaac
     6661 ttctcgctgc aactcagcag cgaagtccgc gccctaaagc gtaatgacga taacacctgg
     6721 accgttaccg ttgccgatct gaaaaacggc actgcacaga acatccgcgc caaatttgtc
     6781 tttatcggcg cgggcggtgc ggcgctgaag ctattacagg aatcggggat tccggaagcg
     6841 aaagactacg ccggtttccc ggtgggcgga cagttccttg tttcggaaaa cccggacgtg
     6901 gttaatcacc atctggcgaa ggtttacggt aaagcatccg ttggcgcacc accgatgtcg
     6961 gttccgcata tcgatacccg cgttctggac ggtaaacgcg tagtgctgtt tgggccattt
     7021 gccaccttct caaccaaatt cctcaaaaac ggttcattgt gggatctgat gagttccacc
     7081 accacctcta acgtgatgcc gatgatgcac gtcgggctgg ataatttcga tctggtgaaa
     7141 tatctggtga gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt gaaagagtac
     7201 tatccgcagg caaaaaaaga ggactggcgt ttgtggcaag cggggcagcg cgtgcagatt
     7261 atcaagcgtg atgccgagaa aggtggcgta ctgcgtctgg gtactgaagt ggtcagtgac
     7321 cagcaaggga ccattgccgc gctgctgggg gcatcgccag gggcgtcaac cgccgcgccg
     7381 attatgttgg atctgctgga aaaagtattt ggcgatcgcg tttccagccc gcaatggcag
     7441 gctacgttga aagcgatcgt tccgtcttat ggacgcaagc tgaacggtga tgtagcggca
     7501 acagaacgcg agttgcagta caccagcgaa gtgctggggc tgaaatacga caagccgcag
     7561 gcagcagata gtacgccaaa accgcagttg aaaccgcaac ccgttcaaaa agaagtggcg
     7621 gatattgcgt tgtaatgatg tgccacatcc ggcatggtat gccggatgtg gcgtatgctg
     7681 ataagacgcg ccagcgtcgc atcaggcacc ggctcgggcg ttagatgtcg gatgcggcgt
     7741 gaccgcctta ttcggcctac ggggtgccgc acttcacagc attttagcga actaccgcgt
     7801 tgtcaatttt ctcttccgcc ttccagacgc ggtacttcac atctacattg tctggcgtat
     7861 acaccacgat cggcagcttg ctgttgtaac gcagcattcc agcatcgccc agatacgcgg
     7921 tgacaaattt cttctctttc ttgccatccg ggcaggccat catcgtggaa accggcgagc
     7981 taactttatc aaagacataa taatcatagc cccagccttc cagcgttttg ctttccagct
     8041 tcccgccgag acgatgcaaa ttgcaatcga cttccagcgt ctgaccgatt aacagttcta
     8101 ctttcagggt agattcatct tcttgcgggg ttaactgaat cacctgacgc ttcatccctt
     8161 tttcagcttg tggataaggc gcgatttttt ccagtggctg gacgctttct gccgcccagg
     8221 cggaagtggt agcgaaagcg gcaaacaata ctgcaggtag aatggtcttc atttttttgc
     8281 tttccttctt catttatagg ttttcaccga tgat
//
LOCUS       DAFAVF010000172         8254 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFAVF010000172 DAFAVF010000000
VERSION     DAFAVF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.174"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1368
                     /gene="yjeM"
                     /locus_tag="LGK42_003863"
     CDS             <1..1368
                     /gene="yjeM"
                     /locus_tag="LGK42_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBK2660055.1"
                     /translation="ALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTF
                     MWFSSYIIWMVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTV
                     VASKGINKIARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQS
                     GLAMLSFVVFAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWG
                     VSTNWQQVLSNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMF
                     LAYTGAFFTLCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLV
                     SFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIA
                     TVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKP
                     ELALD"
     gene            complement(1519..4842)
                     /gene="mscM"
                     /locus_tag="LGK42_003864"
     CDS             complement(1519..4842)
                     /gene="mscM"
                     /locus_tag="LGK42_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBK2660056.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4864..5832)
                     /gene="psd"
                     /locus_tag="LGK42_003865"
     CDS             complement(4864..5832)
                     /gene="psd"
                     /locus_tag="LGK42_003865"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBK2660057.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5929..6981)
                     /gene="rsgA"
                     /locus_tag="LGK42_003866"
     CDS             complement(5929..6981)
                     /gene="rsgA"
                     /locus_tag="LGK42_003866"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBK2660058.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7076..7621
                     /gene="orn"
                     /locus_tag="LGK42_003867"
     CDS             7076..7621
                     /gene="orn"
                     /locus_tag="LGK42_003867"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBK2660059.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7832..7907
                     /locus_tag="LGK42_003868"
     tRNA            7832..7907
                     /locus_tag="LGK42_003868"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7865..7867,aa:Gly,seq:gcc)
     gene            7944..8019
                     /locus_tag="LGK42_003869"
     tRNA            7944..8019
                     /locus_tag="LGK42_003869"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7977..7979,aa:Gly,seq:gcc)
     gene            8055..8130
                     /locus_tag="LGK42_003870"
     tRNA            8055..8130
                     /locus_tag="LGK42_003870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8088..8090,aa:Gly,seq:gcc)
BASE COUNT         1870 a         2146 c         2227 g         2011 t
ORIGIN      
        1 gcattgttat tctttattcc attcgcctta atgatggctg aaatgggagc tgcctatcgc
       61 aaagaagaag gtggtatcta ttcctggatg aataatagtg tcggaccacg ttttgccttc
      121 attggtacgt ttatgtggtt ttcctcttat atcatctgga tggtgagtac ctccgcgaaa
      181 gtttgggtac cgttctcaac attcctctat ggtagcgaca tgacccagca ctggcgtatt
      241 gctggactgg agcctacgca ggtggttggt ctgctggcag tggcatggat gattctggtc
      301 accgtcgttg cttcaaaggg gattaataaa attgcccgca ttactgcggt gggcggtatt
      361 gcagtaatgt gtctgaattt agtattgctg ttagtaagca ttactatttt gttattaaat
      421 ggtgggcatt tcgcgcagga tattaatttc cttgcatcac cgaacccagg ttatcagtcc
      481 ggtctggcaa tgctatcgtt tgtggtattt gctatttttg cctatggcgg aattgaagcg
      541 gttggtggtc tggtcgataa aacggaaaat ccagaaaaga actttgccaa aggtattgtt
      601 tttgccgcta ttgttatttc aatcggttat tcgctggcaa tatttttatg gggtgtcagc
      661 acaaactggc agcaggtatt aagtaatggt tccgttaacc tcggcaatat tacctatgtg
      721 ctgatgaaga gcctcggggt gacgctgggt aacgcactgc atttgtcacc tgaagcgtca
      781 ttgtcgctgg gtgtatggtt tgcgcgtatt accggacttt cgatgttcct cgcttatacc
      841 ggggcgttct ttacgctttg ctattcaccg ctgaaagcca tcatccaggg gacgccgaaa
      901 gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg ggatgccttc tatcgccatg
      961 tggatgcagt gcgggttggt tactgtcttc atcctgctgg tttcgtttgg tggcggtacc
     1021 gcatcggcgt tctttaacaa gctgacgctg atggcgaacg tgtctatgac gcttccttac
     1081 ctgttcctcg cgctggcttt cccgttcttt aaagcacgtc aggatctcga cagaccgttt
     1141 gtgattttca aaacgcattt gtcggcaatg attgcgacag tggttgtcgt actggtggtg
     1201 acatttgcga acgtcttcac catcattcaa cctgtggttg aagccggaga ctgggacagc
     1261 acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc gatttaccag
     1321 aactattgca gtcgcatggc gaataaacct gagttagctc tcgactgatc atatgaaatc
     1381 ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat ggatgatact
     1441 ctccagggcc ggtgataact gaagatgatg ttaagctgct aaatgagcca cgttgacatc
     1501 atttggcagc ttcatgcatt acaaacttcc cgcctgacga cctttgcccg cagaagttaa
     1561 tgttctcccc gtttgcttac cgttgaggct ttccagacgc atctggaagg gcgggaatgg
     1621 catatcgata ccgtgggcgt ggaagcctgc cagaatcagc tggtggatct catggcgtag
     1681 cggcatacgg tgacccatct cagcggcgta aatacgcagc tcgaaaatct gaatcccctg
     1741 ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg agcagcgacg
     1801 cgctgcggtg agcaggattt ccgtcacttc ttcgctattg gcatcggcag gggccggtat
     1861 cgtcaacacc acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat
     1921 aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg tggtggcgcg
     1981 ggtgttaatt ttcgtcacgc taccggtaag atcgcggatc gttaccgtat cgccaatgcg
     2041 aatcggtttt tcgaacagga taatcaggcc agagataaag ttggcgaaaa tctcctgcaa
     2101 accaaaaccg agaccaacac cgagcgcggc aaccagccac tgcaatttcg accactcaat
     2161 accaatcatc gagaagccga ccagcccgcc aatcagcatc agcagatatt tggtgatggt
     2221 ggtgatggcg tagcccgtac ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag
     2281 cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa tggcaatcag
     2341 caccgcaccg agggtaattg gctccagact ttctacgccc tgtaccgtgg aggtgacatc
     2401 ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat
     2461 gacagaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg attgcgcact
     2521 gatggcatcg agatcgactt cgctttcatc gacttcaatt gccccttccg ggctactgtg
     2581 atgatgcgct tcctcttcgc cacgcgcgcg ttgcgctaac atctctgccc ggcgatgctt
     2641 cgcccggtca aacgccagcc tgcgacgctg gatcagcatc cagcggcgga taacgtgata
     2701 aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta acagtgcctg
     2761 cgccgttgcc agataaccca ccgctgacgc cagaatggca accagtggtg cgccaatcat
     2821 catgttccac agcatatggt tggtaatgtt gtcgccgctg ccctctttgt tgaggtacag
     2881 cgggatcccg gcctttttca ggctgagggt gaccaccgcc agcgcaccgc aaatgaggat
     2941 aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat cgaacatcat
     3001 cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc gcatcccacg
     3061 ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg ggcgggcaaa
     3121 ggtggcgcaa atcatcacta cccacagcag cggcacggtg gccgttacgc catcgccaat
     3181 cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg tcatccacag
     3241 caccggcagc ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg tcagccagaa
     3301 gtgatcctga gtcactttgc cgactttcgc cgccgaacgt tcaagaaaac gggtgaaata
     3361 gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg gcaaaatcgt
     3421 ctctttgctg gtcagcatca tcacgctggc tttacccaac tgactgaagg tgtccagcga
     3481 gatgaggcgg cgcagatcct gggcgatttc cagcggccag gcgatagtca tcgggcgcac
     3541 gtcagaggtc cagaacagat agcggtgcgt cgcttcgttc acctctttca gcgcatcctc
     3601 cagttgcccg ttggagactt tcagcttggt cagttccagc agtagcgtgt ctccaccctg
     3661 caacaatgag ttcagcaact cacgctgagt gcgtagctgt gcttccagaa tacggttttg
     3721 ctcggcggtc agcggctgac cgtcggcctg atgaatttgc cttagctgcg gctgtttatt
     3781 aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg tatcaagttg
     3841 ttgtggtttt ggcatttccg gcagccgtgc cacctgcgcc cgcaacgctt cgccgagcag
     3901 gttggacgag cccagccatt gcgactgttc acgcagcgta ttcaacgcct gccggacctg
     3961 taacgtctgg ctggtggcct ggcgctgttg cgaggcaacg agatccatcc gctgcgcctg
     4021 ttgattcagt gccgccgata gttcgcggtt aattttgaat tgcgcgacga tatctttcgg
     4081 caaatcggca ctgttttctg ccagtaattc ggtactttcc agcgcccgct ccgcctcaag
     4141 ctgacgttgg ctattcaact gattacgtaa ggcctgcaaa tacgcatcca gttgctggct
     4201 ctctttttcc gccagctctg agcgcaagcg cgctaattcc tgacggttat tggcagacag
     4261 ctgcgccagt tccagttcat caacgagcgc tttcaggcgt gcggagtcag actgcaacgc
     4321 gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc ggcgctcgat
     4381 ctcatttaac tgacggcggg catcggtttg ctgttgcggc agttgattca gtgaatcggc
     4441 aatctcgcgg gcgcgctcct gctcttgctg ggcctgacgg ctcttatcca gcaactgact
     4501 gctgacctgg agaatttcct gattcagcgc atcggtagac attcccggcg acacgctgcg
     4561 cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt tcgggtaatt
     4621 atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt cctcaagcgc
     4681 atttaaggca gactggagtg cctctatgac ttctggctgc gcgggtttcg ccgcttttgc
     4741 ctgctccagt tcctgggtga tttgtttgct atcgggggcc gtcgcggcgt acgcccccca
     4801 actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt ttcctttgat
     4861 ggattagacc tggtcttttt tgtcgtcaac caatgggctg gcgtcgtgtt ctgcttcgat
     4921 ctcttcagca ggaagcgggg caggttcagc gtctggcgta acaaaggttt cggtagatac
     4981 tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa ccagattcac
     5041 tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac ccatttcctg
     5101 gcctttaagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc agcgcttgat
     5161 gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc tgccaacaat
     5221 cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa ggcaaatcac
     5281 gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt taacggagaa
     5341 gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca tgtgtacgcg
     5401 gtggtagtca cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga acaggtccgc
     5461 catcagatag ttgcctgcca gcaaggcttc gaggctgtag ttgtggcctt tggcttgcag
     5521 gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag gcatgaccag
     5581 gacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga caaagaattc
     5641 gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt cgaccttgta
     5701 gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct tgcttgcgcc
     5761 ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg aaagtttaaa
     5821 tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg gcctacatgc
     5881 cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc agtcatccgt
     5941 atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac ggtgatagtt
     6001 ttcgaaacgg gtttccgcga tttttccttc ctcaaccgct tcccggatag cgcagcccgg
     6061 atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat ggaattcgac
     6121 aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac gcacaccagg
     6181 ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagt cgtgcggcgg tcgtggtgtg
     6241 ctgaccgaga cccgagttgt cagagacatc gttggtcagg atctcttttt gcagccccag
     6301 caacgcgttc agcaggctgg atttgccgac gccagactgg ccggcaaaaa tgctgatgcg
     6361 cccggtcaac gcctcttcca gcggttttag cccatcctga gtatggctgg aaaccatcaa
     6421 tacgcgataa ccaatgttgc ggtagatatc catctgctcg ttgacgaacg ccatgccttc
     6481 gtcgtccagc aggtctatct tattgagcac aataatcggc tcaatctgca aggtttcgca
     6541 ggccaccagg taacggtcga taatattgag cgacagctcc ggcaaaatgg cggagacaat
     6601 gacaatctgg tcgatgttgg cggcaatagg tttcacgccg tcgtagaaat ccggacgcgt
     6661 caacactgag gtgcgctcat gcaccgcttc cacgatccct ttgacattta cgccttccgc
     6721 cgccggttta cccggacgcc agactacgcg gtcgccggtt accagcgaac ggatggtacg
     6781 gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt gcataccaaa
     6841 gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt agtcgggctt
     6901 ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct gctggccttt
     6961 ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg gcaaaacatc
     7021 tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa atagcatgag
     7081 tgccaatgaa aacaacctga tttggatcga tcttgagatg accggtctgg atcccgagcg
     7141 cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata ttctggcaga
     7201 agggccgacc attgcagtac accagtctga tgaacagctg gcgctgatgg atgactggaa
     7261 cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg aaagcgagca cgatgggcga
     7321 tcgggaagct gaactggcaa cgctcgaatt tttaaaacag tgggtgcctg cgggaaaatc
     7381 gccgatttgc ggtaacagca tcggtcagga ccgtcgtttc ctgtttaaat acatgccgga
     7441 gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag agctggcgcg
     7501 ccgctggaag ccggaaattc tggatggttt taccaagcag gggacgcatc aggcgatgga
     7561 tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta tcaagctgta
     7621 aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat tttgacgcta
     7681 aattgaacgt cttgctgata atttcagcac ttgagataaa aactcaaaaa aaatttttta
     7741 ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga cgcagaaatg
     7801 cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg tagagcacga
     7861 ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa tttgaaaagt
     7921 gctgcaaagc acagaccacc caagcgggaa tagctcagtt ggtagagcac gaccttgcca
     7981 aggtcggggt cgcgagttcg agtctcgttt cccgctccaa aatttgaaag tgctgtaagg
     8041 cacagaccac ccaagcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg
     8101 tcgcgagttc gagtctcgtt tcccgctcca aattcttctc tcaataaaat atccacagcg
     8161 atgcgatgcg ttattgctgg ttttcgttgt ctctgacaaa ctcttgtaaa cagagttatc
     8221 cacagcctca ggctgtaatc ttaatttcaa agaa
//
LOCUS       DAFAVF010000173         8179 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFAVF010000173 DAFAVF010000000
VERSION     DAFAVF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.175"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(167..898)
                     /gene="dnaQ"
                     /locus_tag="LGK42_003871"
     CDS             complement(167..898)
                     /gene="dnaQ"
                     /locus_tag="LGK42_003871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBK2660060.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            963..1430
                     /gene="rnhA"
                     /locus_tag="LGK42_003872"
     CDS             963..1430
                     /gene="rnhA"
                     /locus_tag="LGK42_003872"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBK2660061.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1427..2149)
                     /locus_tag="LGK42_003873"
     CDS             complement(1427..2149)
                     /locus_tag="LGK42_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2660062.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2183..2938
                     /gene="gloB"
                     /locus_tag="LGK42_003874"
     CDS             2183..2938
                     /gene="gloB"
                     /locus_tag="LGK42_003874"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBK2660063.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3010..4368
                     /gene="mltD"
                     /locus_tag="LGK42_003875"
     CDS             3010..4368
                     /gene="mltD"
                     /locus_tag="LGK42_003875"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBK2660064.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4416..5186)
                     /locus_tag="LGK42_003876"
     CDS             complement(4416..5186)
                     /locus_tag="LGK42_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK2660065.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5264..6064)
                     /locus_tag="LGK42_003877"
     CDS             complement(5264..6064)
                     /locus_tag="LGK42_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK2660066.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6305..7219
                     /locus_tag="LGK42_003878"
     CDS             6305..7219
                     /locus_tag="LGK42_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2660067.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7216..8019)
                     /gene="dkgB"
                     /locus_tag="LGK42_003879"
     CDS             complement(7216..8019)
                     /gene="dkgB"
                     /locus_tag="LGK42_003879"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBK2660068.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2172 a         1962 c         1991 g         2054 t
ORIGIN      
        1 aggcaggtat tctaaccgac tgaactaccg ctccgcgttg tgttccgttg ggaacgaggc
       61 gaatattacg gattgcctca cccttcgtca acggtttttc tcatcttttg aatcgtttgc
      121 tgcaaaaatc gcccaagccg ctatttttag cgccttttac aggtatttat gcccgccaga
      181 ggcagcttcc gcccttcttc tccaccagat caagacgggc ttcatgagct gcaagctctt
      241 catctgtcgc aaaaacaacg cgtaacttac ttgcctgacg tacaatgcgc tgaattgttg
      301 cttcaccttg ttgctgctgt gtctctcctt ccatcgcaaa agccatcgac gtttgaccac
      361 cggtcatcgc cagataaact tccgcaagga tctgtgcatc gagtaatgcc ccgtgtagcg
      421 ttcgtttact gttatctatt tcgtagcggg cacataacgc atcgaggctg ttgcgcttac
      481 caggaaacat tttcctcgcc accgcaaggc tatcggtgac cttacagaag gtattggtct
      541 tcggaatatc gcgcttaagc aacgaaaact cgtagtccat aaagccgata tcgaacgctg
      601 cgttatggat caccaactcc gcgccgcgga tatagtccat gaactcatcg gctacttcgg
      661 caaacgtggg cttatcgagc aaaaattcat cggcaatacc atgtacgcca aaggcttccg
      721 gatccaccag ccgatcgggt ttgagataaa catggaagtt attgcccgtc aggcgacggt
      781 tcaccacttc aacggcacca atctcaatga tcttgtggcc ttcatagtgc gcaccaatct
      841 ggttcatacc ggtggtttcg gtatcgagaa cgatctggcg tgtaattgca gtgctcatag
      901 cggtcattta tgtcagactt gtcgttttac agttcgattc aattacagga agtctaccag
      961 agatgcttaa acaggtagaa attttcaccg atggttcgtg tctgggtaat cctggacctg
     1021 ggggttacgg cgctatttta cgctatcgcg gacgcgagaa aacctttagc gcaggctaca
     1081 cccgcaccac caacaaccgt atggagttga tggccgctat tgtcgcgctg gaggcgttaa
     1141 aagaacattg cgaagtcatt ttgagtaccg acagccagta tgtccgccaa ggtatcaccc
     1201 agtggatcca taactggaaa aaacgtggct ggaaaacagc agacaaaaaa ccagtaaaaa
     1261 atgtcgatct ctggcaacgt cttgatgctg cattggggca gcatcaaatc aaatgggaat
     1321 gggttaaagg ccatgccgga cacccggaaa acgaacgctg tgatgaactg gctcgtgctg
     1381 cggcaatgaa tcccacactg gaagatacgg gctaccaagt cgaagtttaa gcttgtggtt
     1441 tacgacattg ccgggtggct ccaaccgcct ggcgaattcg tggcttgttt ttactctgtt
     1501 tcatcgggtt tagcgttaaa ggaatagtcc gtttccgggc aacaataagt tgtaagcaac
     1561 caagcgcagg aatatgcgca ttcaatagtt ttcctccgtg tttgttccac gggagaacgt
     1621 ggaaacggct ggcgtgcagc acttcaaaat tcaacaaaga gagccagtcc agttgccgca
     1681 tcagagtaaa catccggctg ttatagggcg aggtttttcg caataccggc acaagtttgc
     1741 gtaatcccat taaactaatg ggattgaagc cactaatcac cagccagcca tcatcaatca
     1801 atacccgatc ggtttcacgc aataaacgat gcggatcggt gcaccacggc aatgtatgtg
     1861 ccagtagaca aacatcaacg gatttatcgg caaaaggaag atgaagtgga tccgcctgca
     1921 cctggacggg cattccttgc gcagaaacat tcacttgatg agaaaccgcg cacgcttcgc
     1981 aattgatttc tgcgcttaaa ttgccaatct taagcagatg aaaaccatac attttagtga
     2041 accacgggtt gagctggcgc tccagcgcct tgcgataaag ctctccccag ggcaaatcgc
     2101 cccagcgatc aggagccacg acagtttgag ggactcttgc cggtttcatc acaaccttcc
     2161 gtttcacact aagaggtaat ctatgaatct taacagtatt cccgcctttg atgacaatta
     2221 catctgggtt ttgaatgatg aagcaggtcg ctgcctgatt gtcgatcccg gagacgcaga
     2281 gccggtatta aacgccattg ccgccaataa ctggcaaccg gaggccatat ttctcaccca
     2341 ccatcatcac gatcacgttg gcggcgtaaa agaactggtg gaaaagtttc cgcaaattgt
     2401 ggtgtatggt ccacaagaga cacaagataa gggaacaaca caggtagtca aagatggcga
     2461 aactgccttc gttttggggc atgaatttag tgtaattgcc acaccgggtc acactttagg
     2521 acatatctgt tacttcagta aaccttatct attttgcggc gacacgctgt tttctggtgg
     2581 gtgtggtagg ttgtttgaag ggacagcatc acaaatgtat caatcactta ataagttaag
     2641 tgcgttacct gacgatacat tggtatgttg tgctcatgaa tatacgttat caaatatgaa
     2701 gtttgctttg agtattcttc cgcacgattt gtccataaat gattattatc gtaaagttaa
     2761 ggagttacgg gcaaaaaatc aaataacact acccgtaatt ctgaaaaatg agcggcaaat
     2821 taatgttttt ttaagaacgg aagatattga tttaattaat gtaattaatg aagaaacatt
     2881 attgcaacaa cctgaagagc gttttgcatg gttaaggtca aagaaagata ggttctgata
     2941 aaactttctt gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt
     3001 gacacacaca tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag
     3061 agtaccggca acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca
     3121 gcaaagttta caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt
     3181 gacttgtggg ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt
     3241 cgcgaacaga aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg
     3301 gcagagccgt atatgtactg gatagcaggg caagttaaaa aacgtaacat gcctatggaa
     3361 ctggtactac tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat
     3421 gccgcaggca tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc
     3481 cgcaattatg acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaatatgatg
     3541 cagcgtctga acaaaatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc
     3601 ggcgaaggtc gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac
     3661 ttctggtcgt taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg
     3721 agtgatattc tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc
     3781 cgtgctctgg cgcgtgtgca cctgagcagc ccggttgaaa tggcgaaggt tgcagatatg
     3841 gcggggattt ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg
     3901 ggcgcaagtg gtccgcaata cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa
     3961 tctctcgctt caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg
     4021 cttaacagcc gtgtttacac cgtacgctct ggcgacacgc tttcaagtat cgcttcacgt
     4081 ctcggcgtaa gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag
     4141 ccaggccaaa gtttgacgat tggtgcaggc agtagcgcac agcgactggc aaacaacagc
     4201 gatagcatta cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac
     4261 ggcgtgaaca tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc
     4321 gacaagctga cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa
     4381 taaaaaggca ccgattcccc cggtgccttt tttatttatg ctgctttatg tgcttctacc
     4441 atgatgatat cactggtgaa agagccatca ttctgcaagg caaaatacgt tctcacctct
     4501 gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt
     4561 ctcgcgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc
     4621 agattggctt cattgattaa cctcaaccac tcaccgctgg cgtagtttcg tacgtgagag
     4681 gtatcgcgta atgcttctac cgtctgtaac cagatgtcgc gcactggatg acccggagac
     4741 attacgtcca tcacaatcat cctaccgcca ggtttcaata tccggttcac ttcacgcagt
     4801 gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg
     4861 ttatcggcaa atggcagact ttcggcataa ccctggcggg tggtgatatt tttcagttgc
     4921 cggacttcgg cagcttgtgc cacgacatcc agcatgtggg cagataagtc atacgccacc
     4981 accgtgctca cgttttgcgc agcgacaaag ctggcatgcc ctgctccgca gcccatatca
     5041 agtacacttg caccaggata atcagccaga cgcaccgcca ggcgctgtaa atctcgtccg
     5101 gatgcatgta cggtactggt cagatattcg caggcttgag agctaaactg cttttctacg
     5161 tggtcatggt gggattgtgt tgtcattgcg ctgtcctttt gttgtttgag tataaaagga
     5221 cagcgccaaa cgttggtctg ccctgtggca gacctgacat accttattta tcaggcttgc
     5281 cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc aatacagaag
     5341 cttcgctgac gttcagacca cggtagaaaa caaaatcgag cgggcggcca aacgcccgac
     5401 ggcgctgatc atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca aagcgatata
     5461 acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg accgggccgc
     5521 tgtggtgagc tatctgatca ccaataggaa gtaactgctt actatagaca tccacgccca
     5581 gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca ggtaatggat
     5641 agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc
     5701 agcaatacac tggatgcgcc gccgaaaggg tcattacacc ggaaggatgt tgtggcagca
     5761 cgaaagcggg cacctgatcg gcggcaagat agttagcggt cgcaaactgt actaactctg
     5821 gcgttgtctg cgcttcctgc aataacacca gatgtgcatc tttgccgtag ttctttaata
     5881 ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc cgaatacgct
     5941 cttcggtact taacggttct ccaggtggta atgcctggcc gatgctcgca aaagaccccg
     6001 gcggtaagat cctttccgca ggttgtccgg caacatagcg catggcatag gtgttttttc
     6061 gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga gggttctact
     6121 gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat gtaagcaagt
     6181 gcttacacag gtcagaattt tgctcattat tgagaacatt aaatcggatg acatatcacg
     6241 catcctgatg gtttaatgac aaataaattc aaaatatctc ttctattttg caacaggagc
     6301 aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtag aaagcggcag
     6361 ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc gggcagtgaa
     6421 aaagctggag atgaaacttg gcgttagcct gcttaatcgg accacgcgac aacttagcct
     6481 gacggaagaa ggccagcgtt attttcgtcg cgtacagtca attttgcagg agatggcagc
     6541 ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttgc gcatcgatgc
     6601 cgcaactcca gtggtgctgc actttctgat gccgttgatt aagcctttcc gtgaacgcta
     6661 tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga tcgaaagaaa
     6721 agtggatgtc gcgatacgcg ctggtacgtt aacggattcc agcttacgtg ccaggccgtt
     6781 atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct acgggaagcc
     6841 agaaacgatc gacgatttaa agcaacatat ttgcctggga ttcactgaac ccgcttccct
     6901 caatacctgg ccgatagccc gtagcgatgg acaattacat gaggtgaagt acggtttgtc
     6961 atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt
     7021 gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt taatggcaga
     7081 taaagtgttg ccagtggaaa tgccattcag tgccgtctat tacagcgacc gtgcggtaag
     7141 tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag ctcccggagg
     7201 agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa ccaggcggtc
     7261 gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta aattttgtgc
     7321 cttaagatta ctttccaggt ttttacgttt agtagaagaa ggaattactg agtaaccttc
     7381 ccccatagcc cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag ctgcgatacg
     7441 agcaataacc tcatctttca gggccttacc atacgccagc gtcatatagg aagtaatatg
     7501 gatgccgtgc tgtttagccc aggcaaccac tttacggttt tgcagataag gagagagttc
     7561 aatctggtta gtagcgatgt tttcagcacc aacagcagca atcgcttttt ccatcaacgg
     7621 gatcgtgaag ttggaaatac cgatctcacg cgtcagccct tgttttttgg cttccagcag
     7681 cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt ggattagcgt
     7741 cagatcaaca taatcggtac gcaatttttg caggctctct ttcagacttg ggatcaattt
     7801 gtctttgctg agattttcaa tccagatttt agtggtgatg tagagttcat gacgtggcac
     7861 gccactttct gcaatcgcct gacctactgc ggcttcgtta tcatagattt gtgcggtatc
     7921 aattgcgcga taaccaagtt caagcgccgt tatcacagat gaaataacaa cgtcgtcttt
     7981 cagacggaaa gtacctaaac caaatgcagg gatagccata atatgcctct tttaatgcta
     8041 ttttcgttaa ctgaggtgat tatgggcttc agactgttga ggaaaaagag cgtaaaatgc
     8101 agaggatttt tgcgattctg gcaataatag atatacagac gaaaaaaaac ctcgagcgtt
     8161 aactcgaggc ttcttaata
//
LOCUS       DAFAVF010000174         7886 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFAVF010000174 DAFAVF010000000
VERSION     DAFAVF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.176"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            125..4033
                     /locus_tag="LGK42_003880"
     CDS             125..4033
                     /locus_tag="LGK42_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBK2660069.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMDISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDMTKLGTGILT
                     LNNDSAAYQGTTDIVGGKLLSVPTLPLIWQVNTLISITAV"
     gene            3994..5544
                     /locus_tag="LGK42_003881"
     CDS             3994..5544
                     /locus_tag="LGK42_003881"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2660070.1"
                     /translation="MASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLE
                     NGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTR
                     VRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWY
                     LTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLG
                     EPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQ
                     DRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWA
                     LYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQAQIT
                     WMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWI
                     NNSKVYAVKMNGQTVGHEGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQG
                     MLGVKYSW"
     gene            5753..6019
                     /gene="hipB"
                     /locus_tag="LGK42_003882"
     CDS             5753..6019
                     /gene="hipB"
                     /locus_tag="LGK42_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBK2660071.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6019..7341
                     /gene="hipA"
                     /locus_tag="LGK42_003883"
     CDS             6019..7341
                     /gene="hipA"
                     /locus_tag="LGK42_003883"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HBK2660072.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7653..7861
                     /locus_tag="LGK42_003884"
                     /pseudo
     CDS             7653..7861
                     /locus_tag="LGK42_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2148 a         1692 c         2176 g         1870 t
ORIGIN      
        1 attgacacct tcccacctcc aactaacact caatacagac ctctgacaat ttaataagtt
       61 tcatttttaa tgtctgacga ctcatctctt tcgtggctgt attgtttgta tatggagcgt
      121 ttatatgaat agaatctatc gcgtgatatg gaattgcact ctacaggtat ttcaggcctg
      181 ctcggaatta actcgcaggg caggtaaaac atcgacggtt aatttgcgta aatcctctgg
      241 actgacaacg aaattcagta gattgacgct gggtgttttg ctggcactaa gcggttcagc
      301 gtctggtgca agtctggaag ttgataatga tcagattacc aatattgata ctgatgttgc
      361 ttatgatgcc tacctggttg gctggtatgg cactggagtg cttaatattt tggctggcgg
      421 taatgcctcc ttaaccacta ttactaccag cgtcattggc gctaatgagg actcagaggg
      481 caccgttaat gttttgggtg gcacctggcg attgtatgat agcggaaata atgcaaggcc
      541 tttaaatgtg ggtcaatccg gaacggggac gctgaatatt aaacagaagg gtcacgtcga
      601 tggaggctat ttaagattag gttcttcgac aggaggcgtc gggacggtca atgttgaggg
      661 agaggactct gttctgacga ccgaattatt cgaaataggg agttacggta cgggctcatt
      721 aaatattacg gataagggtt acgtcacgag ttcaatcgtc gccattttag gctatcaggc
      781 gggcagtaat ggccaggttg tcgttgaaaa gggtggcgag tggctaataa aaaataacga
      841 ttcctcaatt gaatttcaaa ttggtaatca aggaacgggg gaagcgacta ttcgcgaggg
      901 tggactgatt acggctgaaa acacgattat cggtggcaat gctaccggta tcggaaccct
      961 gaatgtgcag gatcaagact ctgtcatcac ggtacgcaga ctctataatg gatatttcgg
     1021 taatggcaca ctcaatattt ccaataatgg actaattaat aacaaagaat attcattagt
     1081 gggcgttcag gacggttccc acggtgtcgt caacgtgacc gataaagggc attggaattt
     1141 cctcggaacg ggcgaagctt tccgctatat ctatatcggt gatgctggcg acggtgaact
     1201 taatgtctcg cgtgaaggta aagtagattc gggaattatc actgcgggga tgaaagaaac
     1261 aggcacaggc aacattactg ttaaggataa gaactccgtt atcactaatc tcggaactaa
     1321 tcttggttat gacggccacg gcgaaatgga tatcagtaat gaggggcttg ttgtcagcaa
     1381 cggaggaagc tcactcggtt atggagaaac cggcgtcggg aatgtcagca tcaccacggg
     1441 ggggatgtgg gaggtcaata agaatgtcta taccaccatt ggtgttgcgg gcgtcggaaa
     1501 cctcaatatt agcgatggcg gtaagttcgt atcgcaaaat attacttttt tgggcgataa
     1561 agcaagcggt atcggcacac tgaacctgat ggatgcgaca tcatcgttcg atactgtggg
     1621 tatcaatgtc ggtaattttg gtagcggtat cgtaaatgtc agtaatggtg ccacccttaa
     1681 ttcaacgggc tatggattta tcggaggaaa tgcctccggt aaggggatag ttaatatttc
     1741 aacggacagt ctctggaatt taaagacatc atctactaac gcacaattgc tacaggtcgg
     1801 tgtattaggc aagggtgaac tgaatattac caccggaggt atagttaaag cgcgtgatac
     1861 acagatagct ctcaatgaca aaagtaaggg cgacgtgagg gtggatgggc agaactctct
     1921 tcttgaaaca ttcaatatgt acgtagggac atctggtacg ggtacgttaa ccctgacgaa
     1981 tagcggcacg ctgaatgtcg aaggtggaga agtttactta ggtgtttttg aacctgctgt
     2041 aggaacgcta aacattggtg cagctcacgg tgaggcggcg gcagatgccg ggtatataac
     2101 caacgcgacg aaagtagagt ttggttctgg cgaaggtgtt tttgtcttta atcatactaa
     2161 taacagtgat gccggctacc aggtcgatat gctgattaca ggtgacgata aagacggaaa
     2221 agtgatccat gatgcaggcc atacggtgtt caatgcaggg aatacttata gcggtaaaac
     2281 gctggtcaat gacggcctcc tgaccattgc gtctcatacg gcagatgggg taacgggcat
     2341 gggttcgagt gaagtcacca ttgcaagccc cggtacgctc gacattctcg catcaacgaa
     2401 cagtgcagga gattacacgc tgaccaatgc gctcaaaggc gatggcttga tgcgagtgca
     2461 gctgtcatcc tacgacaaga tgtttggctt tacccatgca acagggactg aattcgccgg
     2521 tgttgcccaa ctgaaagaca gtaccttcac tctggaacgc gacaacaccg ctgcgcttac
     2581 tcacgcgatg ttgcagtctg acagtgaaaa taccacatcg gtaaaagtgg gagagcaatc
     2641 cattggtgga ctggctatga atggcggtac gctcattttc gatacggata ttcctgctgc
     2701 gacgcttgca gagggatata tcagcgtcga tacgctggtt gtcggcgcgg gtgactacac
     2761 ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac gtgcttatcg acgtgcctaa
     2821 accgtggaat gatcccatgg cgaataaccc tctgacgacg ctcaatttgc tggaacacga
     2881 cgatagccat gtcggcgttc aactggtgaa ggcgcaaacg gttattgggt cgggtggctc
     2941 attaacgtta cgtgatttac agggcgacga ggtggaagcg gacaaaacgt tacacattgc
     3001 gcaaaacgga acggtggtcg ccgagggtga ttatggattc cgcctcacga ccgcaccagg
     3061 tgatggtttg tacgttaact atgggctgaa agcgctgaac atccatggtg ggcaaaagct
     3121 gacgttagcc gaacatggcg gagcctatgg cgcaacggcc gatatgtcgg caaaaatcgg
     3181 tggtgaaggg gatctggcaa tcaatacggt gcgacaggtt tcgctttcca acggtcagaa
     3241 cgactatcag ggggcaacct acgttcagat ggggacatta cgtaccgatg cggatggtgc
     3301 acttggcaac acccgggaac tgaacatcag caacgcggcc atcgtcgatc ttaatggatc
     3361 gacgcagacg gtagagacat tcaccgggca gatgggttcg actgttttgt tcaaagaggg
     3421 ggcgctgacg gtaaataaag gtgggatcag tcagggtgaa ctgacaggtg gcggaaacct
     3481 gaatgttaca gggggaacgc tggctatcga ggggcttaat gcacgctaca atgcgttaac
     3541 cagcattagc ccaaatgcgg aagtcagcct cgataatact caggggttag gcagaggaaa
     3601 tattgccaat gacggtctgt taacgctaaa aaacgtgact ggcgaactgc gtaatagcat
     3661 aagcgggaag ggtatcgtga gcgcaaccgc caggacagat gtagagctgg atggcgataa
     3721 tagccgcttt gtggggcaat tcaacattga tacaggcagc gcgctcagcg tcaacgagca
     3781 gaaaaacctg ggtgatgctt ccgttatcaa taatggcctg ctcaccatct ccactgagcg
     3841 tagctgggcg atgacgcaca gtatcagcgg tagcggtgat atgacaaaac tgggtaccgg
     3901 gatcctgact cttaacaacg attccgcggc gtatcagggt acgacggata tcgtgggggg
     3961 gaaattgctt tcggttccga ctctgccatt aatatggcaa gtcaacacat taatatccat
     4021 aacagcggtg tgatgtcggg aaatgtcacc actgcaggtg atgtgaacgt tatgcctggg
     4081 gggacactgc gtgtcgctaa aaccacaatc ggcggcaacc tggagaatgg cggcacggtt
     4141 caaatgaata gcgaaggggg gaaaccgggg aatgtactga ccgttaacgg caactatacc
     4201 ggaaacaatg gcctgatgac gttcaacgcg acgctgggcg gcgataattc gcccaccgat
     4261 aaaatgaacg tgaaaggcga tacccaaggg aacactcgcg ttcgggttga taacattggc
     4321 ggcgtcggtg cacaaacggt caacggtatt gaactcattg aggttggcgg taattctgca
     4381 ggtaacttcg cgctgaccac cggaactgtc gaagctgggg cttacgtcta cacgctggct
     4441 aaagggaagg ggaatgacga gaaaaactgg tatctgacca gtaaatggga cggcgtaacg
     4501 ccagcggata cgcccgaccc catcaataat ccccctgttg tggatccgga aggcccatca
     4561 gtttatcgcc cggaggccgg aagctatatc agcaacattg ccgcagccaa ctcgctgttt
     4621 agccatcgtt tacacgaccg tctgggtgag ccgcagtata tagattcact gcattctcag
     4681 gggtcggcaa gcagtatgtg gatgcgtcat gtcggagggc acgaacgttc aagggccggt
     4741 gacggtcagc taaatactca ggctaaccgc tatgtattgc agctaggcgg cgatttggcg
     4801 cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg ttacgctaat
     4861 cagcacagta atactcagag taatcgtgtg ggttataaat cggatgggcg catcagcggt
     4921 tacagcgctg ggctgtacgc gacctggtat cagaacgatg cgaataagac cggcgcttat
     4981 gttgacagct gggcgctgta taactggttt gataacagcg tcagttccga taaccgttct
     5041 gctgacgact atgattctcg cggtgtgacg gcctctgttg agggtgggta tacctttgaa
     5101 gcgggaacat ttagcggcag cgaagggacg ctgaatacct ggtacgtcca gccacaggcg
     5161 caaatcacct ggatgggtgt gaaagattcc gaccataccc ggaaagacgg aacgcgcatt
     5221 gaaacggaag gcgacggaaa tgtgcaaacg cgacttgggg tgaaaaccta cctgaacagc
     5281 catcaccagc gtgacgatgg taaacagcgt gagttccagc cttacattga agcgaattgg
     5341 atcaacaaca gcaaagtcta cgccgtgaag atgaatggtc aaaccgtagg ccatgaaggt
     5401 gcgcgtaatc tcggtgaagt acgtaccggg gttgaggcga aagtaaataa caaccttagc
     5461 ctgtggggga atgtcggtgt gcaactaggt gataaaggct atagcgatac tcagggcatg
     5521 ctgggagtga aatatagctg gtaaaccgta taagccgcat gtcgagatgg catgcggctt
     5581 aatattgccg acttcaaagg ctttaaagcc tcccttttat ccgcgatcgc ggatatcgca
     5641 gcgtttatcc cgtacagcgg ataagatgtg tttccagatt gactttatcc tcactaaagg
     5701 ataaaactga taatatcccc ttaagcggat aaatttgctg tggacgtatg acatgatgag
     5761 ctttcagaag atctatagcc caacgcaatt agcgaatgca atgaaactgg ttcgccagca
     5821 aaatggctgg acgcagagcg agctggcgaa aaaaattggc attaagcagg cgacaatttc
     5881 caatttcgaa aacaaccctg acaataccac gctcacgaca ttttttaaga ttttacagtc
     5941 gcttgaactc tcaatgacac tatgcgacac gaaaaatgcc tcgccagaat caacagaaca
     6001 gcaagatctg gagtggtaat gcctaaactt gtcacttgga tgaacaacca gcgggtaggc
     6061 gagttaacga agttagccaa cggcgcgcac acctttaagt atgcaccgga gtggttagcc
     6121 agccgttatg ccagaccgtt gtcactttcg ctgccattgc agagggggaa tatcacctca
     6181 gatgccgtat ttaacttctt cgataacctg ttacccgata gcccgattgt acgtgaccgg
     6241 atcgttaaac gttatcatgc taaatccaga caaccgtttg atttattgtc agaaataggg
     6301 cgagacagcg ttggtgccgt aacgttatta cccgaaaatg aaaccataac gcgcccgata
     6361 atggcatggg aaaagcttac tgaagccagg cttgaagaag tattaacggc ttataaagca
     6421 gatatccctc tgggcatgat tagagaagaa aatgactttc gcatctcggt tgctggcgcg
     6481 caggagaaga cagcgctgct tagaataggc aatgactggt gcattccgaa aggaataacg
     6541 ccgacgacgc acatcattaa attaccgatt ggcgaaatca ggcagcccaa tgcgacgctc
     6601 gatctcagcc aaagcgttga taatgagtat tactgtctgc tgctggcgaa agaacttggg
     6661 ttgaatgttc cggacgcaga aatcattaaa gcgggaaatg tgcgcgcgtt agcggtcgaa
     6721 cgttttgaca ggcgttggaa tgctgagcga acggttttac ttcgcttgcc acaggaggat
     6781 atgtgtcaga cattcggttt accttcatcg gtgaaatatg aatcagatgg aggcccaggc
     6841 atcgcgcgga tcatggcttt tttgatgggg tccagcgagg cgctgaaaga tcgctatgat
     6901 tttatgaaat tccaggtctt ccagtggttg attggcgcaa cggacggtca tgcaaaaaac
     6961 ttctccgtat ttattcaggc tggcggcagt tatcgactca cgccatttta cgacatcatt
     7021 tcagcatttc cggtccttgg cggtacggga atacacatca gcgatctcaa actggcaatg
     7081 gggcttaacg catccaaagg caaaaaaacg gcaatcgata aaatttatcc gcgacatttt
     7141 ttggcgacag caaaggtgct gagattcccg gaagtgcaga tgcatgaaat cctaagtgac
     7201 tttgccagaa tgattccggc agcactggat aacgtgaaga cttcattacc gacagatttt
     7261 ccggagaacg tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc
     7321 cgagaatacg gtagtaagtg agatatgggg tttggtcgtt gttaatgagc acgacaatca
     7381 tgaccgccaa cgactggatt cttccttggt ttatactggt gcgcataatg tatattatgt
     7441 taaatcatgt ctgagtgatg cgaagtgctt ttctggtagt cgttattcgt tcagggttca
     7501 agaacttaag cctctcctct ttcataattg gcctgtgcat ggagaaatag cctggttatt
     7561 ccctgcccca tgctaagagc gcagtgtctg tcgcatagac actgcatacg aaaacaccag
     7621 agggtgctgt tacttattta aggacggatc aagcgggaat gagtccactc cggtatcaga
     7681 aatggctacg tctcaatgaa gtcaggcgac cgatgctgaa tgaacattac gatgtcacca
     7741 cagcagccta tgctgtcggt tacgaaagct atcccatttc agtcgggaat attcgcggat
     7801 gtttggagag tcacccaaga gagatattac caggttaaga aagtctgtcg gtcagcttta
     7861 ataaaccctg cacttatctg tttaga
//
LOCUS       DAFAVF010000175         7761 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFAVF010000175 DAFAVF010000000
VERSION     DAFAVF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.177"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..2378)
                     /locus_tag="LGK42_003885"
     CDS             complement(<1..2378)
                     /locus_tag="LGK42_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660073.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVTRNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATAAGNSVKAAKT
                     SETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAMGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAA"
     gene            complement(2443..3042)
                     /locus_tag="LGK42_003886"
     CDS             complement(2443..3042)
                     /locus_tag="LGK42_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2660074.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(3113..6610)
                     /locus_tag="LGK42_003887"
     CDS             complement(3113..6610)
                     /locus_tag="LGK42_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2660075.1"
                     /translation="MGKGSSKGHTPRETKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGSPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVKHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGRERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            complement(6671..7303)
                     /locus_tag="LGK42_003888"
     CDS             complement(6671..7303)
                     /locus_tag="LGK42_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2660076.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(7240..>7761)
                     /locus_tag="LGK42_003889"
     CDS             complement(7240..>7761)
                     /locus_tag="LGK42_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK2660077.1"
                     /translation="PWLSEADRRLQVQSDLPWWLVCRGTIHKFRCVPHLTGRRFEHGV
                     TDCYTLFRDAYHLAGIEMPDFHRGDNWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVL
                     LCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GIYNDLVAASTFV"
BASE COUNT         1540 a         2365 c         1991 g         1865 t
ORIGIN      
        1 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcatagcca
       61 gacggaacgg tatctgatgg ccacgggatt ggtgcgccag gcggataaaa ctgctctgat
      121 ggcgtataga gtgaataaac tgtaccgtcc gttaaccctt ccggcttatt agcagaatat
      181 gctggtgacg tatgaatcgt cacgctggca ttactggtat aatcccattg aatatttaca
      241 ccagtcgcat aatttccgat tgcaacgtaa atatcgtaag tatcaccaga tgtattgacc
      301 caggcaaaat ttgtaaaccc tgtcgatgtg cgctgccata aagcaccagt aatccccttc
      361 ggattaccat tacctgcacg taaaacaagt tcagatatac ctgcctgttg aggtgacccc
      421 acgttaaatc cagcgccacc aatcaacgta attgaaacaa cagaactcgc ctgtggcatg
      481 gttaccgttg ctaatttgaa ccaaccagca ccaccgctga atgacattgt tgttgagtta
      541 agcgtaccaa tatctttcgg cgtcagtgtt atatccgctg aaagcgcctt accattcacc
      601 ttacgggcag aaggtacccg accattcgca ttgtcattag ctgctttcac tgctttcggt
      661 gtcgcagcaa gcgtttcaga tgtgctgttg gttgcactac tgagctggac aattcctttt
      721 tgtgctgtcg tagcgtcctg tgcggtgtat ttcccgttag ccaggtcata cgcggcctta
      781 acggcttttg gcgttgccgc ctgtgactcg gaagtgctgt tagtcgcact gctgagctga
      841 actatccctt tctgtgctgt cgttgcatcc tgtgcggtgt atttcccgtt agccaggtca
      901 tacgcggcct taacggcttt tggcgttgcc gcctgtgact cggaagtgct gttggtcgca
      961 ctgctgagct gtactatccc ctttttcgtc gtgctcgcat cctcaagcgc cacggcggat
     1021 gcaatatcct ctgcccgttt tgctgctgtc tcggcgcgcg ttgccgcgga ttccgccgta
     1081 cttttgctct gtgctgccgc tgccgcactg ccagcagcct ctgtcgcctt cgtggatgcc
     1141 gtcgtggcgc tgcccatcgc tgctgacgcc tgtctggtcg cctcatcttt tgaagcagac
     1201 gcagatgatg ccgatgacgc cgccgaactg gcggacgatg cggcagccgt ttttgaggat
     1261 tctgcgctgg tttccgacgc tttcgcgttc gtttcggatg tcttcgctgc ggaagcagac
     1321 ctcgctgctg cgctggcctg ttcagtggct tcgccagcct tcgttgtggc tgttgaagcg
     1381 gatgatgcgg cgctttctgc cgattttccg gcggcggtgg cactgactgc tgcctcctct
     1441 gccttaatgg acgcctcacg ggccgatgat gcagctgctg tctcagagtc tgccgcagct
     1501 gaggcgctct gttccgctgc cgtttcagaa gacctggcat tcgtctcgga cgtttttgcc
     1561 gccttcacgg aatttcctgc cgccgttgcc gaggaggctg cactgctggc gcttgtggag
     1621 gcgttcgttt ctgatgattt tgccgcctct ttggaggccg ccgcatctct ggctgaagtg
     1681 gcggcttctg acgctttcgt cgtcgcagtg gatgcagaag tggctgctga tttttgtgac
     1741 gctgcagcat tcgtttctga cgttttcgcc gcaccggcac tggtggccgc cgcgcttttt
     1801 gaggactctg cagcggcagc actttttgat gcttcagtgg cctttgttga tgccgttcct
     1861 gcgctggaag acgcctcctg agccgaagtc gcggcctgtc cggctgacgt gctggctgca
     1921 cgcgctgagc ctgcagcatc agtcgcatgg gttgccgcct cacgggcaga tgtgccggca
     1981 tcactggctg acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgtc
     2041 acctcttcca ccatcagttc aaaacggcgc agagcctccg gacgggcatc atcctccgtc
     2101 atggcaccga gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac ggtgatggtc
     2161 ccggcatgtg acggcgggaa tccttccacc aacagaataa cgctgtactg accgtactca
     2221 acgtccatgc tgtaacgccc ggcttcatcc ggattttctg aggccagcgt gttcaccacc
     2281 accgtggtgc tgttacgttt tgctttcagc tggattgtgc agttctgtac cggttttcct
     2341 gtgccgtctt tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg
     2401 cctgaaccgg cgggctgtca taacactgtg ttacctggct aatcagaact tataaccgac
     2461 acccacgatg aaaccgtcag tgcgccagtc gccactgccg gagccttcat aagcaatatc
     2521 aatggccacg gattcggtcg ggttaaactg cacgccagct ccccacgcca gagacgtgtt
     2581 gctgtggcga ccgtcatcac ttccggtcag cacgtcgtgc gttttcccct tgttgtcagt
     2641 tacgcggaga taatccccgg agaaagtcga cacacggctg taagccacac ccgccatcgc
     2701 atacgcgctg aaccattcat tcacgcgcac agacggcccc gccattacgc tgaaccagcg
     2761 gttacgaacg gaatcttcat gccagcgagt atcgctgtaa tgggtcagct ggcgattctt
     2821 gtctcctgca tagctgaatg acgtcaccag ccccagcgta tccgtaaact cataacggta
     2881 tttcacgtta atcccgttca gatcatcact gccgggaacg ttggtccggg catgaagata
     2941 ccccgcgctc agcgtggact gatgttcaga cgcccatgca ggcgcaccgg atacggccag
     3001 acaaatggct gcggacaaaa tggcggcaca aactttacgc ataattacct ctcgcttttc
     3061 tgcaataaaa aaggcgtcat tcctgacgcc atttattggg gttataaata tttcaacgaa
     3121 tactgatgcc ggaagccgct tttttggtca caatcaccgt acagtcggtg atattgcctg
     3181 ccccctgatt gcctttctgg aaaatcttaa actccagagt gacgctacca ccaccactag
     3241 gcatatcaat aactgcactg taactaccgg gaatggcccc tttagtttct ctggatgcga
     3301 ttaatacgcc gtttttgcga acttcaaaac cataacccgt gtatcgcgtg cctcccgggt
     3361 tatttccgct ccccggatcg tcatacgcta taccgttaaa aataatgggc ggaataataa
     3421 tctggcggtc aaagttatga tcatcgctga tggtgactgt aaccgtaccg tttggtgttt
     3481 ccgtgttacc ccacgtaccg acttttttcg ggaaggcttt tgatacagct ttaacgaaat
     3541 ctcctctgac ctgggtcgcc tccagcatgc ccttaatcgt acagttctgg ttaatcgtga
     3601 cattgttgag cgttcctgag ttcgcattca cactgccact gatatccgca tttttcgccg
     3661 tcagtctccc gtctgatgtc agggaaaatg ccggaggatt accgccgctg gtaatggtgg
     3721 gggccgtcag gcgtttcagg aacacttcat tcatgaatat ctgatcgccc tgaccaacaa
     3781 acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct gctgccagca
     3841 gcacctgact ctgcatgccg tcaggggtgt tctcaatacc ggcaccgata cccgcaatat
     3901 aaaggcgtcc gtcctgcatc tgctgcagct tcactgccca catgctgttc aggttattat
     3961 ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc actctggtct tccagtttct
     4021 tattgacggt ctgtgttatt tcattgctga catccgttat ggacgtcctg atttcagtca
     4081 ggtcaggcgc aagctgaccg ttatcaatct gcgtccacag ctcctgagcc agatgggttt
     4141 tccctatctc gcctttgaaa aaatccagat agcctgatgc atcatcactc ggctgaccga
     4201 cagcctccac gaatgccgat ttgccaacgg tgttcacact gcgaacgtaa aaataataat
     4261 catggcccgg tttgatattg atactggcgg ctatccagta cagtgccgta ccaagataac
     4321 gcgcgctggt ttcaacctgc ctgatatccg caatccgttt ttccgagaac cagaactcaa
     4381 actgtaccgt cgggtcataa accgcaagat gcggcgtggc ggttatctga aaatagcccg
     4441 gcgtcagctc aatccgcgac ggcgctgccg gtgcggcaat ccggaacgat accgacgccg
     4501 gatcgccctg ctgcccccac gcatttactg cccggactgt cagcctgtag ttccccagag
     4561 ccagttgtgt gaagcggtaa gtggtttccg tcgtccgggc cgtgctgacc agccgctcac
     4621 ggccgtcatc cgctgccacg gtcaggcgaa gcaggaagct cacgcccttc accaccttcg
     4681 gcgtgtccca gcgcgccagc acctggtatt ccccgctgtc tgcggtgact tctgcggtca
     4741 ggtgcttcac cgctggcggc gtgacaccat ttaccgtgcc gctctggtcg ccgtcaaagt
     4801 gcgccccgtt atccacgatg gcctcttttt ccggtacatg ctgcacggcg gtgatggcat
     4861 acgtgccgtc gtcgttctca cggatactca cgcagcggaa caggcgctgg cgcaacgtcg
     4921 gcagcttcag cccccatacg ctgtattcag caacgccgtc aggaacacgg ctcactttca
     4981 ccttcacgcc gtcggtgacg gactgaacct ccacgctgac cggactcccc tgcccgtcaa
     5041 ccaggcttat cagcgtggtg ccggaagatg gcagcgtgat ttcacggtcg agcgtcagcg
     5101 tccgggtctg gctgtttacc gccagcacgc gcccgccggt gcggataccg gcatagtcat
     5161 catcgcagat ttcaatgaca tcgcccggta catggcgaag cccttcggca cccacgctga
     5221 agtccacggt ctgcgtttcc agcagttctg ttttaatcag ccacagcccg gcgcggtgtg
     5281 cctgcccccg gctggtacag ccaaaagcat ccatcttcgt gacgttacga ccgtaacggg
     5341 caatggcctg cgtgtcctcc acaagctctg tcgccgtctc ccagccgtta ttcgggtcaa
     5401 tccagttcac ctcaacggca ttatggcggt ccttcagggc gctgaagctg tagcggaacg
     5461 gcgcaccatc atccggcatc accacattac tgcggttata ggtccacacc ttatccgacg
     5521 gtcggtcctg cacgaacgtc agcgtctgcc cgttccatac cggcatacag cgcatcgcag
     5581 agcagaaatc actgagcaca tcccacgcct tgcgctgtgt ggtcaggtac gcattacagg
     5641 tgatgcgcgg ctccgtgccg ccaaaaccgt ccggcaccga ctgatcgcaa tgctggccga
     5701 tgacatacag cgcccatttg tccacatccg ccgcaccaag acgtttcccc atgccgtagc
     5761 gcggatgggt cagcatatcc cacagacacc aggccatgtt gttgctgtat gctggcttaa
     5821 acgttccgtc ccagataccg ctgtattgcc gcgtctgcgg gttatagttc gacggcacct
     5881 gcagaatgcg cccgcgaaga tgataattac ggctcacctg ctggctgccg aactgctccg
     5941 aatccacctg tacgccgacc agtgccgtat tcgggtagca ctgtttcaca tcgatgattt
     6001 cggtgtatga cgaccagagc gttttgttct gcagctggtc tgtggtgctg tccggcgtca
     6061 tcctgcgcat ccggatactg aacgggcgcg gcggcaggtt atccaccacc accgaggcca
     6121 gatactgcga ggtggttttg cccttaatgg tgatgtcttt ttccgtcacc cagccaccat
     6181 tacgctgtat ctgaaccagc aggcgaactt ccgacggatt ccggtccccc tttgaggtgg
     6241 tttccaccag tgcctgcaca ccgaaggtaa agcgcagtcg gtcaatgttt gccgacgtga
     6301 tggtccgggt gatcggcgtg tcgtatttca cttccgtacc cagcaccgtc tcggagccgg
     6361 aggattcaaa tccctccggc ggtgtctgct cctgctcacc ggcccggaac accaccgtga
     6421 cgccggagat gttggtattc ccctcactgt ccagcactgg cgtactgttc agcagcacgc
     6481 tttttaatcc atccaccgga ccttcaaccg gcccttcgct gatggcatcg atcacactca
     6541 gcaactgcgt ggacttcagg ttgtccttcg tttcgcgcgg agtatgcccc ttactgctgc
     6601 ctttacccat tcctcacgct ccataaacga caaaaccgcc cggaagcggt ttcacataaa
     6661 acgtttttca tcaacgacca atcaccacaa cctgaccacc gtccccttcg tctgccgtgc
     6721 tgatctcctg agagaccacc cgcgacccca cgcgcatttc accgtacaga acgggcagaa
     6781 cattgccctg ggcaaccatg ttatccagtg aggagaaata ggtgttctgc ttaccgttat
     6841 ccgttgtctg tgtacgggga gttctggctt tcggtgccag catctgcgcc acaccaccga
     6901 gcaccatact ggcaccgaga gaaaacagga tgccggtcat accaccggcc ccaatggctg
     6961 ccccccatgc tgcaagggtg gctccggcgg taaagaatga tccggcaatg gcggcagccc
     7021 ccaggacaat ctggaatacg ccacctgact tggccccggc gactctggga acaatatgaa
     7081 tcacagcgcc atcaggcaga gtctcatgta actgagccgt taacccggac gtgctgacgt
     7141 cccgcccggc aatccgtacc tgataccagc cgtcgctcag tttctgacga aacgccggga
     7201 gctgtgtggc cagtgcccgg atggcttcag cccccgtttt cacacgaagg tcgatgcggc
     7261 gaccaaatcg ttgtaaatcc ccgtaaaggc agatgcgcgc catgcccggt gacgccagag
     7321 ggagtgtgtg cgtcgctgcc atttgtcggt gtacctctct cgtttgctca gttgttcagg
     7381 aatatggtgc agcagctcgc cgtcgccgca gtaaattgcg gcgtgattcg gcaccgatga
     7441 accaaaacag cacagcagca catcgcccgg ctgcgccgct gacaacggca cctgatacag
     7501 ccctgtggcc tccagattat ccagatagag attctgaccg ttacgccacc agttatcccc
     7561 gcgatgaaaa tccggcatct caatccccgc cagatgataa gcatcccgga acagtgtgta
     7621 acagtccgtc acaccgtgct caaagcgccg cccggtgaga tgcggcacac agcggaactt
     7681 atgaatcgtc ccccggcaga ccagccacca cggcaaatca ctctgcacct gcagccgccg
     7741 gtcggcctca ctcagccagg g
//
LOCUS       DAFAVF010000176         7652 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFAVF010000176 DAFAVF010000000
VERSION     DAFAVF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.178"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="LGK42_003890"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="LGK42_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBK2660078.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="LGK42_003891"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="LGK42_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBK2660079.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="LGK42_003892"
     CDS             complement(2659..5772)
                     /locus_tag="LGK42_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBK2660080.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="LGK42_003893"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="LGK42_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBK2660081.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="LGK42_003894"
     CDS             7014..7292
                     /locus_tag="LGK42_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660082.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..>7652)
                     /gene="gadE"
                     /locus_tag="LGK42_003895"
     CDS             complement(7293..>7652)
                     /gene="gadE"
                     /locus_tag="LGK42_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBK2660083.1"
                     /translation="AIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLE
                     ITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKL
                     KLRRMSDIVTLGITSYF"
BASE COUNT         1972 a         1793 c         1837 g         2050 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggtatagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gc
//
LOCUS       DAFAVF010000177         7568 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFAVF010000177 DAFAVF010000000
VERSION     DAFAVF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.179"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(9..272)
                     /gene="cedA"
                     /locus_tag="LGK42_003896"
     CDS             complement(9..272)
                     /gene="cedA"
                     /locus_tag="LGK42_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBK2660084.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRSKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            455..2716
                     /gene="katE"
                     /locus_tag="LGK42_003897"
     CDS             455..2716
                     /gene="katE"
                     /locus_tag="LGK42_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBK2660085.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     HLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2763..3521)
                     /gene="chbG"
                     /locus_tag="LGK42_003898"
     CDS             complement(2763..3521)
                     /gene="chbG"
                     /locus_tag="LGK42_003898"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBK2660086.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3534..4886)
                     /gene="celF"
                     /locus_tag="LGK42_003899"
                     /gene_synonym="chbF"
     CDS             complement(3534..4886)
                     /gene="celF"
                     /locus_tag="LGK42_003899"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK2660087.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(4991..5833)
                     /gene="chbR"
                     /locus_tag="LGK42_003900"
     CDS             complement(4991..5833)
                     /gene="chbR"
                     /locus_tag="LGK42_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBK2660088.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5841..6191)
                     /gene="chbA"
                     /locus_tag="LGK42_003901"
     CDS             complement(5841..6191)
                     /gene="chbA"
                     /locus_tag="LGK42_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBK2660089.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKIKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6242..>7568)
                     /gene="chbC"
                     /locus_tag="LGK42_003902"
     CDS             complement(6242..>7568)
                     /gene="chbC"
                     /locus_tag="LGK42_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBK2660090.1"
                     /translation="LLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLSF
                     GEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVD
                     ALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRNW
                     VIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASL
                     GSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAAG
                     KTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPI
                     LFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTN
                     GSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2021 a         1993 c         1688 g         1866 t
ORIGIN      
        1 tgtaatcctt actcagtcac ttccccttcc tggcggatct gatttgccca acgttgggca
       61 gattcaggca cagtaaacgc cggtgagcgc agaaatgact ctcccatcag tacaaacgca
      121 acatatttgc tacgcagcat ccagacatca cgaaacgaat ccatctttat cgcatgttct
      181 ggcggcgcgg gttccgtgcg tgggacatag ctaataatct ggcggttttg ctggcggagc
      241 ggtttcttca ttactggctt cactaaacgc atattaaaaa ttagaaaaac tgtagtttag
      301 ccgatttagc ccctgtacgt cccgctttgc gtgtatttca taacaccgtt tccagaatag
      361 tctccgaagc gggatctggc tggtgatcta tagttagaga gttttttgac caaaacagcg
      421 gccctttcag taataaatta aggagacgag ttcaatgtcg caacataacg aaaagaaccc
      481 acatcagcac cagtcaccac tacacgattc cagcgaagcg aaaccgggga tggactcact
      541 ggcacctgag gacggatctc atcgtccagc ggctgaacca acaccgccag gtgcacaacc
      601 taccgcccct gggagcctga aagcccctga tacacgtaac gaaaaactta attctctgga
      661 agacgtacgc aaaggcagtg aaaattatgc gctgaccact aatcagggcg tgcgcatcgc
      721 cgatgatcaa aactcactgc gtgccggtaa ccgtggtcca acgctgctgg aagattttat
      781 tctgcgcgag aaaatcaccc actttgacca tgagcgcatc ccggaacgta ttgttcatgc
      841 acgcggatca gcagctcacg gttatttcca gccatataaa agcttaagcg atatcaccaa
      901 agcggatttc ctctcagatc cgaacaaaat caccccagta tttgttaggt tctctaccgt
      961 tcagggtggc gctggctctg ccgataccgt gcgtgatatc cgtggttttg ccaccaagtt
     1021 ctatactgaa gagggtattt ttgacctcgt tggcaataac acgccaatct tctttatcca
     1081 ggatgcgcat aaattccccg attttgttca tgcggtaaaa ccagaaccac actgggcaat
     1141 tccacaaggg caaagcgccc acgatacttt ctgggattat gtttctctgc aacctgaaac
     1201 tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc ccccgcagtt accgcaccat
     1261 ggaaggcttc ggtattcaca ccttccgcct gattaatgcc gaagggaagg caacgtttgt
     1321 acgtttccac tggaaaccac tggcaggtaa agcctcactc gtttgggatg aagcacaaaa
     1381 actcaccgga cgtgacccgg acttccaccg ccgcgagttg tgggaagcca ttgaagcagg
     1441 cgattttccg gaatacgaac tgggcttcca gttgattcct gaagaagatg aattcaagtt
     1501 cgacttcgat cttctcgatc caaccaaact gatcccggaa gaactggtgc ccgttcagcg
     1561 tgtcggcaaa atggtgctca atcgcaatcc ggataacttc tttgccgaaa acgaacaagc
     1621 agcattccat ccaggtcata ttgttcctgg tctggacttc accaacgatc cgctgttgca
     1681 gggacgtttg ttctcctata ccgatacaca aatcagtcgt cttggtgggc cgaatttcca
     1741 tgagattccg attaaccgtc cgacctgccc ttaccacaac ttccagcgtg atggcatgca
     1801 tcgtatgggg atcgacacta acccggcgaa ttacgaaccg aactcgatca acgataactg
     1861 gccgcgcgaa acaccgccgg gaccgaaacg cggcggtttt gaatcatatc aggaacgcgt
     1921 ggaaggaaat aaagttcgtg agcgcagtcc atcgtttggc gaatattatt cccatccgca
     1981 tctgttctgg ctaagtcaga cgccattcga gcagcgccat attgtcgatg gtttcagttt
     2041 tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc gttgttgacc agctggcgca
     2101 tattgatctc actctggccc aggcggtggc gaaaaatctc ggtatcgaac tgactgacga
     2161 ccagctgaat atcaccccac ctccggacgt caacggtctg aaaaaggatc catccttaag
     2221 tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga
     2281 tgaagtgaga tcggcagacc ttctggccat tctcaaggcc ctgaaggcca aaggcgttca
     2341 tgccaaactg ctctactccc gaatgggtga agtgactgcg gatgacggta cggtgttgcc
     2401 tatagccgct acctttgccg gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg
     2461 cggcaatatc gcggatatcg ctgacaacgg cgatgccaac tactacctga tggaagccta
     2521 caaacacctt aaaccgattg cgctggcggg agacgcgcgc aagtttaaag caacaatcaa
     2581 ggtcgctgac cagggtgaag aagggattgt ggaagctgac agcgctgacg gtagttttat
     2641 ggatgaactg ctaacgctga tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga
     2701 caaaattccg gcgtaaatat ggataaggtc acgtcccctg gggagtgacc ttaccacctt
     2761 acttacacat caagataact acccaaccga taaccacgct cagcaattgc atatttcaac
     2821 gacgctgatg tcagcacttc cagctccgtt aaccgtggga aacagtaagc actcttgcga
     2881 atcaaattat cgacaaacgc cggatggcac atgacttcca gcgagcgttc cctgcgatgg
     2941 ctggaatcat ccagcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg
     3001 ctactgaatc cctgcgaact gcgcagatta gccggtaaat caccagcgtt agatagcggc
     3061 tgacggtcga tgcgcaacgc aatcccctct tcagccgcaa acctgacaac aatcgggaaa
     3121 atctgcggga acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat
     3181 agctcaatga aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa
     3241 gcatcttctt ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc
     3301 ggcatagctg tcagtggctt gcccatagta aggacaaagt gcatccctat ggccagactc
     3361 ggttcatcac gactcaactg caccgcatgg tcaatagcct gcccattcac cagcgccgtc
     3421 gtcgatgtga caatcccatt gcgacaggcc tcgataatgc cgtagttctg acctttgctt
     3481 aagccaaaat catcggcatt aacaatcagt aagcgttcca taatcagcct cggttaatgt
     3541 gcttttttaa gctctgcgat gcagtcggca aaatttggca gccatttctc gtgcgccaga
     3601 atcatctcgc gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg actaaggttt
     3661 agcgccagta acacatcgtt aaattctccg ctaagtgcgg cgttgctggc agcaatctcg
     3721 aagcctttaa tggtgtgaat cagccccatc actttatcat cgaaatgcgt aatgcgcgga
     3781 tgtggcgtcg cgccatcgcg ccccagctta caggtcattt ctaccgccca gtctgccgga
     3841 atattatcaa tatgcccatg atgcgggata ttaacgtaat gttcagcttg cttgtcgttg
     3901 tagatagcgt tgatcacttc gcacgctgca tcagagtaat aagccccacc gcgctgttcc
     3961 agttctttcg gcttaacttt caactccgga tttttataca gctcaaaaag ttgtttctcg
     4021 actttctgta ctacctgtgc tcgtgcgccg cctttgtagt attcgcccat ttcaatagcc
     4081 agcatctctt tctgcttgaa gtaatacagc agataagaac atggcagcag atttaacgaa
     4141 cgaattaagc cctcactaaa tggcagatcg aaaatatttt ttacagagga cgcttttaac
     4201 tgccctgacg ccacaccatc aagcaattcg gcaaagcgcg acttgccatt taccagcaca
     4261 tccttaatga acaccatatg gttgaggccg aacagatcga tagataaatc atcgctgtct
     4321 ttcagcatca gaacatcgcg aataaacatc ttcatgccga tcggaatatt acacacgccg
     4381 ataaagcgtt taaatccggt atgacgataa acggcttcag tgaccattcc cgccgggtta
     4441 gtgaagttaa tcacccatgc attcggacaa agttcttcga catcttttac gatgtcaaaa
     4501 atcaccggaa tggtacgcag acctttaaac agaccgcccg cgccgttggt ttcctgacca
     4561 agataaccat gacttaatgg aatacgttca tccagttcgc gcgccggtaa ttggcctacg
     4621 cgcagttggg tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc cagcgtttta
     4681 taaagcttca tcgggacgcc agcgttatca atcatccgtt ggcagagttc aaaaatgata
     4741 tccagtttct ctttaccatc ttcgacatcc accagccata attcgctgac cggcaattcg
     4801 tgataacgct taataaatcc ttccagtaac tccggggtat agctgctccc gccaccaata
     4861 gtgacgactt ttaatttctg gctcataatt tctcccttca gtacagaata ctgatatctg
     4921 gcatatctgc ccccccggac ataaataatc cagcaacagt acagatatgt gaattgtcag
     4981 gtataacgac ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg
     5041 acgttaattt cttaaacgtt ttaataaaca aactcgggct actataaccg gcctcaaacg
     5101 caatatccgt cacggaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga
     5161 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
     5221 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
     5281 ctttatcatg catcttttct accgtacttt tcagccactg cggtacatca tcaatcacct
     5341 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
     5401 cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa
     5461 aagcgttatt ggtccggtat acctgcgaag cgacaaagca ataaggcaac aacggcaggt
     5521 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaact
     5581 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
     5641 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
     5701 aatagtcgtg ctgatgcagc ccgctgatac tctcagtttt gttatagata aacacatgga
     5761 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
     5821 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
     5881 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
     5941 ctcactttaa tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
     6001 gcttcattca atgccatccg tgactgatcc atcatggctt ttgctgcagc aaaatcgccc
     6061 tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga gttgatgata
     6121 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     6181 agatccatca tacatcgttc ctctctcttc ttaccggcac gattacccgt accggcatcg
     6241 attaaaattt cagggcgtta gcgatatctt cttcgctttc ttctttatca atcgcatttt
     6301 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg ccaaggttga
     6361 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     6421 ttggcatggt ccacggtgca atattggtca ccggaggaat aatgcccatg tagtacgctg
     6481 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     6541 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     6601 gcgccagttt tgccacctga cgataatcag cacggcgaga tgcgataaag atagccagga
     6661 tcaggcctaa agtcgcacca ctgccaccga ggaaaataaa ggagtccagc atcggcttgg
     6721 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     6781 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     6841 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     6901 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     6961 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     7021 agccgggaat taatgccgag aaggaacgcg atactgaagc aggtacgctg tcgggcagtt
     7081 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     7141 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     7201 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaacccg gccgccagcg
     7261 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     7321 ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     7381 caatggttga ggcgtcgagg cgaataccta aggaataaaa aaacgacccc tccccaaagc
     7441 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
     7501 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
     7561 ggaggagt
//
LOCUS       DAFAVF010000178         7563 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFAVF010000178 DAFAVF010000000
VERSION     DAFAVF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.180"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(224..1564)
                     /locus_tag="LGK42_003903"
     CDS             complement(224..1564)
                     /locus_tag="LGK42_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBK2660091.1"
                     /translation="MIKETVTMSHKELHRLQIIQESINRHITQEQAAARIGISIRQVK
                     RLVQRYRNEGPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREI
                     HGLSLSVETLRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRG
                     PRCTLIVFIDDATSALMALRFVPAETTRAYMETLRGYLNDHGVPLALYSDRHSIFRVN
                     NPEREGELTQFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIE
                     TANAWLPTFIEAYNNRFATSPRTTDNAHLDVHHSEEELGYIFSLQAKRVLSKNLTFQY
                     KSSAFQVRSESRGYRLRHSVVTVCENFDGEINVLYDGKALGWEKYVDGPEPIPLDDEK
                     SVHERVDNARIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKKD"
     gene            complement(2333..2619)
                     /locus_tag="LGK42_003904"
                     /pseudo
     CDS             complement(2333..2619)
                     /locus_tag="LGK42_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2785..2967)
                     /locus_tag="LGK42_003905"
                     /pseudo
     CDS             complement(2785..2967)
                     /locus_tag="LGK42_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3230..3586)
                     /locus_tag="LGK42_003906"
     CDS             complement(3230..3586)
                     /locus_tag="LGK42_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK2660092.1"
                     /translation="MSAPATILDMCCGSRMFWFDKSDKRAIFSDIRKEGYTLRNGRRL
                     IISPDIIADFRALSFADASFSMVVLDPPHLERVGDNAWMGKKYGRLNKDAWRDDLRQR
                     FKEAFRVLRPHGVLIF"
     gene            complement(3583..4005)
                     /locus_tag="LGK42_003907"
     CDS             complement(3583..4005)
                     /locus_tag="LGK42_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBK2660093.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAMVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            complement(4046..5011)
                     /locus_tag="LGK42_003908"
     CDS             complement(4046..5011)
                     /locus_tag="LGK42_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660094.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDSLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(4992..5513)
                     /locus_tag="LGK42_003909"
     CDS             complement(4992..5513)
                     /locus_tag="LGK42_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660095.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(5497..5724)
                     /locus_tag="LGK42_003910"
     CDS             complement(5497..5724)
                     /locus_tag="LGK42_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2660096.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            5806..6213
                     /locus_tag="LGK42_003911"
     CDS             5806..6213
                     /locus_tag="LGK42_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660097.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            6382..6537
                     /locus_tag="LGK42_003912"
     CDS             6382..6537
                     /locus_tag="LGK42_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBK2660098.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNPGE"
     gene            6697..6915
                     /locus_tag="LGK42_003913"
     CDS             6697..6915
                     /locus_tag="LGK42_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660099.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANETRAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            7483..>7563
                     /gene="dicB"
                     /locus_tag="LGK42_003914"
                     /gene_synonym="ftsT"
     CDS             7483..>7563
                     /gene="dicB"
                     /locus_tag="LGK42_003914"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBK2660100.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFT"
BASE COUNT         1968 a         1915 c         1593 g         2087 t
ORIGIN      
        1 acagtccaca aaactgccaa cttccgcttc ttgctcttag cggacattag cataggctat
       61 ttaccataac gcctcattac gcgcaccgcc cagactgact cagcgcgttt ctggcatatc
      121 cccggtaaaa caagtaacaa accacccgaa aatgaacacc agaaacgcga cttaagaatc
      181 taccctatga atggatatgc actcaaccga atcgatcttg gtttcaatct tttttatcgg
      241 gatcaggctt ctttttaggt aacttcgggg gcttaacttg ctgatgactt tgcgttcggc
      301 gcgtaagcca gggatggtca gctttaggtt taacatagta ttttgagcgt aaatcaatac
      361 gggcattatc cactcgttca tggacactct tttcatcatc cagtggtata ggctccgggc
      421 catcaacata cttttcccag cccagcgctt tcccgtcata cagaacgtta atttcaccgt
      481 caaagttctc gcatacagta acaaccgaat gcctaagtcg atatccccgg ctctcactgc
      541 gtacctgaaa cgcactgctt ttgtactgga aagtgagatt tttagacaga acgcgcttcg
      601 cctgtaggct gaagatataa cccagttcct cttcagaatg gtgcacatca agatgagcat
      661 tatcagtagt acgaggcgac gtagcgaacc ggttgttata ggcttcaata aaggtcggca
      721 accatgcatt tgctgtttca atatcactga tattctgaag ccgcatttct ttgaccagcc
      781 tgtcctgtag tgtctgattg gcgcgctcta cccgcccttt tgcctgcggg ctgttggcat
      841 ggattggctc gatgcccagt gtctttatcg cacgagtgaa ctgggtcagc tcaccttccc
      901 gctctgggtt atttaccctg aatatactgt gtctatcaga gtagagagcg agcggtacgc
      961 catgatcatt aaggtaaccc cggagggttt ccatgtaagc ccgggttgtt tcagcaggca
     1021 caaaacgcaa cgccatcaac gcactggtgg catcatcaat gaaaacgatc agtgtacatc
     1081 tggggcctcg attttcaaac cagtcatgag gtgagccatc aatctggatc agttcaccgt
     1141 aagatggtcg tcgcatgcgg cgttgatata tacgggcaat tttacgacgg cgttcacgcc
     1201 ataacccctc ttctatcatc cactttctga gagtttcaac ggataaagat aaaccgtgta
     1261 tctcgcgcaa tttttcgcac gcaaacgtag gtccaaaatc agcgtaacgg cctttgagga
     1321 gtgaaattac tgttgctctg aattcagtag aaaaggaatt attaggacgc tttccacgtc
     1381 ggtgggaaac cagaccagaa ggcccttcat ttctataccg ttgcaccaga cgtttaacct
     1441 gccgaataga aatgccaatg cgtgccgcag cttgttcctg agtaatatgt cgattaattg
     1501 actcctgaat aatctgaagt cggtggagtt ccttatgact catcgtaaca gtctctttga
     1561 tcatgaaaaa tcccccagag aattgtctgg ggacatttta gaatggttca aaggggacat
     1621 tacagcttgg tgttaacaat agcctgcttc tccgtgcctt tcggcacgta agaggctaac
     1681 ctacatgtgt ctagcatgaa attggcctca gattaatgtt aagcgtcttg caggacgcgt
     1741 aatgttaact ggggcttttc tctatctgcc gttggtgtcc atgcccgagg cagatagcct
     1801 caagcacccg cagtcattct acttaactaa gatttccccg caaaccgttt ttgtccagca
     1861 cagtaaatat ccaactaaac caatggagtt cgctgtattt accgccagta ttcaatgcac
     1921 atgaccgcca tgaacacccc taaaaaaagg gcatttatat atccaaatat taatatcaaa
     1981 acatcaactt tttccatata ccttgctgtg aagatgatgg gcatacatga tgcgaacaac
     2041 cagaacgcaa caaacaaaaa ctgcaatgcg tttttcatta ttcctcctac aatcaatgtg
     2101 caattacatt taaacacacc tcaatttggc cggacatata aatatctaaa ccagaaaaaa
     2161 tcacttacat agcgttacaa actctttagt ctaaaggttc atcgtaaaac attccccata
     2221 cttatcagtc cgttccgcgc caggtagctc attgccttat ctggcaacct gtaatcaggt
     2281 ttccgctttt tcagttggct ggtcgtttaa ccgacatagt taatccatta atctagttgc
     2341 cggatgttgg tggattttcg cgttttagtt gttcataaaa gtgcacagct ttaaccagtt
     2401 cttctgatgt aaccgggact ggtgaggcag tgaataaggc ctgaatttga tagttcggcc
     2461 tgtcgttaca atcctctttt ttcggtacat atttccagtc accagaccac tacttcccct
     2521 gaaagtccgt aacgcttttt tcacgtagcg atatcgccat gccactggtt ttgcttgccc
     2581 cgccgtttca tgcccttcct gataattaat ctcgctcatt catcgcccca ctcatcacaa
     2641 tatgcttcga ccggagtttt tcccgcttca tagtcatcac gttaggattc gacatcagca
     2701 gcacttccac cgcgtaattc accatagtcc attaatagtt catcccgctc ttcaaaactg
     2761 atgttatatt tagctgaaca aaaatcagcc actttgttct tcctcatcgt cttttatttc
     2821 gtggtatgag taattgcagt agttaaagaa aatttcttat gctccgtcat gaatttcatc
     2881 aggtgttgcg tcatcgtcca cttcgaatac atcctcaaaa tctccaccag ctatttccgt
     2941 ttcaataata attttgaact ttcgcatttc actaccgccc tttcgggcgg cctcctgatg
     3001 ttctgagggt gcagaaatcc atccggttaa ggattaagtt ttatttacag aactgaattt
     3061 aattattcag atatacgtat ctgtaacctt acgaatctac tcactggatg cctctttcat
     3121 aaaaataatc cagtgggttt tatcgttttt tccctgttcg ttgacagata acaggctttc
     3181 tatctgtcag tgccagaatc tggcttaccg gtatttgcgt ttcattccat taaaaaatca
     3241 gaacgccgtg cggcctcaac acccgaaaag cttctttaaa tctctgtcgc aaatcatcac
     3301 gccaggcatc tttattcagc cgtccatatt tctttcccat ccaggcgtta tcaccaacac
     3361 gctcaagatg cggagggtct aatacaacca tcgaaaaaga tgcgtctgca aatgataatg
     3421 cacgaaaatc tgcgataatg tcagggctga taatcaagcg tctcccattg cgtaatgtgt
     3481 atccttcttt tctgatatcg ctgaatatcg cccgtttgtc agacttatcg aaccagaaca
     3541 tgcggctgcc acagcacata tcaagaatgg ttgccggtgc actcactgcg ccacgtcctg
     3601 aaaattaccc tgatagaaag ccagtatgcg ctgcataact tcgctcttcc ggcactcgcg
     3661 acagattatg tttaggcgac tgtcgtagcg acgtatttct ccgtcaggtg atgaccagat
     3721 aaggtccgga tcaaccatag caggtttctt cacctttgcc ctcgagagtt ttttgcgggc
     3781 gttttgccag tccttacgcg cctgttcaga ggggaataac ccgtagccgg agttgtatac
     3841 atcgccactg gctaccagct ctctggcaag aacgctcatc agatatctag tcgcacctgt
     3901 tttagcttcc agttgccgta acgtctcacg gccgctctgg cgcacgagtt ccaccacctg
     3961 ccatttaatt ttttcccgct cttcttgtgt aaaaactttt gccacaagtc ccccttaaaa
     4021 ttacctcatg acctgaaatc aacacttatc ctctgaaacc aggcggaatt tctgtatccg
     4081 gttcagaaat atgattaaca caacgctgta caggtgaacg tcccaggcgg atgaccagtt
     4141 cgtcccattt ttcgcggagc tttgacgggc tcatgatgtt ttttacccag aatggatctc
     4201 gctgaacccg accaaacatt tcgcaaattt gtctgtggct tctgccatcc agcatccgca
     4261 ttgtgcgcac gtcattggcc caggcagtcc agttaggctc ttttggtcgc atgatctcgc
     4321 catcatcact ggctgcctgt tcgtagagac ccacgatccg cccccagatc cactgcgcac
     4381 acgccaaatc ctccctgcta ccccactggc gttttttcgc actaaacaca accgcgtcgg
     4441 agttccgggt taaagaatcc tgttcagtcg tctgcgggtc cggttgcgaa gcttccggac
     4501 gaaaagtgtt tttattctct gtagtaatct ctgttgtatt ctctgtaaga tcatcaggcc
     4561 attttgaccc gatgacattg ggtcgttttg aaccaatgga gcgtttcatt ttgacctctt
     4621 ccatcgtgtc attttgacct gatggagcgg cgcattttga accgatggat tcgctcaatt
     4681 tgccaccatc taaaagctcg ctcccatagt tgatcgtgta gaaattggtc atatcgcgct
     4741 ttgatttatt gagcttttca caacgcaaaa gccccagcgt tttcagactt gcaaacgcgc
     4801 gctttaacgt tgactctgac cagaatggga actgttccag ccattgttcc gttgtgttat
     4861 aaatccagcg aacaccatca cattccatgc cggagttggt atctctcaac cagtagtgca
     4921 gttgttgcaa aacaatggct tcgtttaagc caattttcat tgccagctgc gtgtttataa
     4981 ccagtgggcg ttcagcaaaa agaaggctca taattccatc cagctttttg ttggtattgc
     5041 tgtcgatacg caagcttgaa agcaattgct ttttctataa gttcgtcagt ttcacgatcc
     5101 actacggcag gatcagcaaa aagcagtccg gactccacca catcgccata ttctttgttt
     5161 aacccggcga tcatgcacgt gatgcttttt ccgtcagtaa tttcacgata caacctgaaa
     5221 tcactaatcc ggatagcctc cataatcgca ggcactagcg ccgtgaactt ttcacgctta
     5281 tccctggtgt cgatagcctt ccagcgttcg aatatcttca ctcgattaac accaagcgct
     5341 cgctgatcaa ccgcgccacc ttcatctgtg acacgctgaa catcgatgtt cgggcgctct
     5401 ttcaaagccc agaatgcttc agtgattaat atcgtcgcct gctcctgtgt cattcctggt
     5461 cgacatatcc aggcatccag agcctcacga gcctgttcag gagtgatttt cattgttcaa
     5521 ccgccccgcc cgcttcgtct tacgatattc atcataaact ttgggatcat actgaagctc
     5581 cccgccagat gcctcctgta gacgcatcgc gcgaccttca ggaaccagta tcccccaagc
     5641 agcaacactt gccagcctca ctcctgcggc attggcaagc tttgttttgc tgccaaaaaa
     5701 agtaattgcg tcaactttaa gcatcaaagc ccccctcttg ttagactttt ctaacattat
     5761 tgtgcgcggg atacctaagt caagaaaaat tagaattacc taactatgga tacaagaacc
     5821 ctgggccagc gagttctggc gcgacgaaaa gaattacgct taacacaacg agaagccgcg
     5881 cgcctcgctg gagttgctca cgtcacaatt tcacaatggg aaagagacga aacccagcca
     5941 gtcggaaaac gattgtttgc tttagcggat gctctgaagt gctcacctac atggctaatg
     6001 tttggtgacg aagacaaggc accagtgcct gcacaagaac ttcatgtgga aacagagcta
     6061 actcccaacc acaaagaatt gatcgaatta ttcgatgctc ttccatcttc cgagcaggaa
     6121 gccttgctgt ctgaaatgcg cgcaagagta gaaaacttca acaaactctt cgaagaaatg
     6181 cttaaagcgc gtaaaaataa atcaataaaa taacattctt ttcaagtgat tagttgcgcc
     6241 cacccttttt gttagatcaa tctaacaaaa aacacttgcc tctcatgtta ggttattcta
     6301 aattattttc catcaagaca ccgcacggtg ttctcagcaa acagttccgc taccccggcg
     6361 ttaaggggaa atgaggtcag catggatact atcgatcttg gcaacagcga atctctggta
     6421 tgtggcgtgt tccccaacca ggacggtacg ttcaccgcga tgacgtatac caaaagcaaa
     6481 acgtttaaaa ccgaaaatgg tgcccgtcgc tggctggaaa gaaacccagg tgagtgatat
     6541 ggatttcgac acaatcatgg aaaaggctta cgaagaatac ttcgaaggtc ttgccgacgg
     6601 cgaagaagct ctcagcttca acgaatttaa acaggcgctt tccagttcgg caaaatctaa
     6661 cggctgataa gcgaaacagc accgcgagga atcagtatgc agaaacgaga acccgtcatc
     6721 atcgcgccag actataccga tgatgaactt tatgagtgga tgcgccagaa aattaatgca
     6781 gcgcaggatt tgaaatgggc caatgaaacc agggctaagc aggctgaaaa tctgtccgct
     6841 ctggagcagg atatcaccaa tctggaaaaa gcagcggcat taagcattgc cagaatgatt
     6901 acatacccgc gttagtagct aatcaacaaa gctaaggtta gtaattaagg agttctccac
     6961 gggtgaggtg gagtgcgtgc gccggacacg ggtgagcatc cggcactgac agtttactga
     7021 aaggatattt ccctgaaaag tcagaccata acgcgaaagc gcacggcgag gtagctggtt
     7081 catagatagc ctgtcgttaa attttcgtcg accgtgcgct tccggttgtg gcaacccgcg
     7141 aaatggcgcg gcggtaagta tggcggggtt attccttccc cgttgaggac accgggttgt
     7201 caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta tcaattttct
     7261 ggtgacgttt ggcggtatca gttttactcc gtgactgctc tgccgccctt tttaaagtga
     7321 attttgtgat gtggtgaatg cggctgagcg cacgcggaac agttaaaacc aaaaacagtg
     7381 ttatgggtgg attctctgta tccggcgtta attgttaact ggttaacgtc acctggaggc
     7441 accaggcact gcatcacaaa attcattgtt gaggacgcga taatgaaaac gttattacca
     7501 aacgttaata cgtctgaagg ttgttttgaa attggtgtca ctatcagtaa cccagtattt
     7561 act
//
LOCUS       DAFAVF010000179         7534 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFAVF010000179 DAFAVF010000000
VERSION     DAFAVF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.181"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            67..500
                     /locus_tag="LGK42_003915"
                     /pseudo
     CDS             67..500
                     /locus_tag="LGK42_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(469..2205)
                     /gene="aceK"
                     /locus_tag="LGK42_003916"
     CDS             complement(469..2205)
                     /gene="aceK"
                     /locus_tag="LGK42_003916"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBK2660101.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2486..3790)
                     /gene="aceA"
                     /locus_tag="LGK42_003917"
     CDS             complement(2486..3790)
                     /gene="aceA"
                     /locus_tag="LGK42_003917"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBK2660102.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3820..5421)
                     /gene="aceB"
                     /locus_tag="LGK42_003918"
     CDS             complement(3820..5421)
                     /gene="aceB"
                     /locus_tag="LGK42_003918"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBK2660103.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5690..6619)
                     /gene="metA"
                     /locus_tag="LGK42_003919"
     CDS             complement(5690..6619)
                     /gene="metA"
                     /locus_tag="LGK42_003919"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBK2660104.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6776..7219
                     /locus_tag="LGK42_003920"
     CDS             6776..7219
                     /locus_tag="LGK42_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK2660105.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(7337..7452)
                     /gene="rrf"
                     /locus_tag="LGK42_003921"
     rRNA            complement(7337..7452)
                     /gene="rrf"
                     /locus_tag="LGK42_003921"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1827 a         1935 c         1922 g         1850 t
ORIGIN      
        1 ctgatgattc aactccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat
       61 aatagtggac atatgaacgt tattaatact atttttaacg cattacccac tctttttaat
      121 acgtttaaat tcgataaaaa aatatgaagc ccctcctcct ggcaaataat tctaatgaat
      181 atcccggttt gttttcagcg atacagcata aacaacaaaa cgttgtagag acggtttatc
      241 ttgctttatc tgaccatgca cgcctgtttg gatttaccgc tgaagatatt atggattttt
      301 ggcaacacaa agccccacaa aaatactctg cctttgagtt ggcttttgaa ttgggtcacc
      361 gggttattgc tgaattaatc cttaatacat taaataagat ggctgaaagc tttggcttta
      421 cggataaccc tcgatacatt gcggagaaaa attatatgga agctttactc aaaaaagcat
      481 ctccccatac cgtacgctaa atctttgcct gcgccgatac gcataaacat cttccacatg
      541 cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg
      601 catctcttca aacagcggac caatacgcgg gtcggcgcat agccagtggc gaaactcttc
      661 cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt cgtcttccgg
      721 atagcgcggc ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta
      781 atcataaaaa accacacgcc cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc
      841 aggaaaaatg ttagcagcgg caagctggcg aatagcgtta ccgtattctt caatggcgtc
      901 gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca tccgccgctc
      961 gatatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc
     1021 ctggagcagt aattccatta atgccgggga aatatgccgc ttctccagca caaagttttc
     1081 aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca
     1141 ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt
     1201 gaatacccga tcaaagcccg gcagcgtaaa taccaacatc accattccac gaatacccgg
     1261 cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc
     1321 ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg
     1381 cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata
     1441 agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat
     1501 aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg
     1561 cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac
     1621 ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat
     1681 gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac
     1741 gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg
     1801 aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag
     1861 cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa
     1921 gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc
     1981 gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg
     2041 atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc
     2101 gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc
     2161 ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca
     2221 ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt
     2281 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
     2341 ggcgtaaatg ccttatccgg cctacgttcg gcaacggctg taggcatgat aagacgcgca
     2401 agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtgaacgcc ttatccggcc
     2461 tacagtcagc aacggttgtt gttgcttaga actgcgattc ttcagtggag ccggtcagcg
     2521 cggtgactga agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac
     2581 ccacttcctg ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct
     2641 gctgcacttt ctcaacgtag tgcttcatac cctcgccctg ggcataggcg tttgccaggt
     2701 caaacatgtt gaaccacatg ctgtggatac ctgccagggt gatgaactgg aacttgtagc
     2761 ccatatccga cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc
     2821 agttgaacga cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag
     2881 cttgtgcaaa gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt
     2941 cagcatatgg cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac
     3001 ggaagaagcc ttcactggta cgctcgccag taataaattc actgtcatac gggtcgcaat
     3061 cggaggtaat cagatccgcc gcatcggcat cggtacgagc aaccagcagg gttggaacgc
     3121 ccgtcacgtc agctgcaaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca
     3181 ccaaaacttt gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt
     3241 gaactgccgc tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac
     3301 cgccaaaacc ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat
     3361 cgcccggctc aatgcctgcg gaccattgga tctgatcggc acgacggaag gtgttgttga
     3421 tccgctccac cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc
     3481 tggccgccag gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac
     3541 ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc
     3601 cttttttcga ctcgccatgc agcagacgcc acattttcgc cgcgcccagt tgcgccaggg
     3661 tgcattcagg attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg tatgggcgag
     3721 taatgccttc ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac
     3781 gggttttcat gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc
     3841 tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc
     3901 ggcatcgtca aaacgcccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt
     3961 catctcttca cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa
     4021 cgttttttga tgatggatcc actgccagat tgaggtacgg gaaatttcag ccgtcgccgc
     4081 atcttccatc aggccataaa tcggcacgca gccgttgccg gagatccatg cttcgatgta
     4141 ctgcactgcc acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac catcacaagg
     4201 tgccagcagc tgatcggcag taatcggcac gtcttgttcg cgcatcactt caagctgatt
     4261 tttacgggag ccgagaatgt cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg
     4321 agcaatccat gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt
     4381 gttgagcacc tggttattgc gctcttcatc tttgctcgga ataaacgctg ccatgccgcc
     4441 catcgcaaaa gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt aagcattcag
     4501 gaatggttta tccatcgtca ctgcctgtct gtctggcagg acgcgatcgg gatagttttt
     4561 caacgttttg atatagctga agatgtaatc ccaacgaccg cagttcagac caacaatatg
     4621 gtcacgcagc gcgtgaagga tttcatccat ctggaacacg gcgggcagcg tttcaatcag
     4681 caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac
     4741 ttcgctccac caggccgctt cctgccagga ctgggttttc ggcagataga aatagggacc
     4801 gctgcccttt gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag
     4861 gctaccgggg attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg
     4921 tacccgacaa atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt
     4981 ggtatagctg atggtgccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt
     5041 gttccagtct ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt
     5101 cagtgcgttg atcaccatct tgcgctcaac cggaccagtt atctcgactc gacgatcttg
     5161 taagtccgcg ggaataccac gaattttcca gtccgtatcg cgaatggaag ctgtttccga
     5221 aataaaatca ggcaaagttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag
     5281 aagtttattg cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc
     5341 ggcagtaaga atttgcttct cctgctcgcc atacggcctt atgaaagcca gttcatcggt
     5401 tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttccccact
     5461 gtgaacgaag gatcgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat
     5521 gcaaaatcaa aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat
     5581 atgaataaaa tttattcacc tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg
     5641 tgcctgaggt aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg
     5701 ttggattcat gtgccgtaga tcgtatggcg tgatctgata gacgtaatag ttgagccagt
     5761 tggtaaacag caaattaccg tgactacgcc agctcgctcg cggtgtattt tgcggatcat
     5821 tgtgcgggaa atagttatac ggtacttccg ggcctagtcc ggcttccaca tcgcggaaat
     5881 attcctgcgc cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct
     5941 tatctttgct ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat
     6001 cggtgtaatc acgaatcaac gctgccggaa agtcagcata gcgcgaatgt ggtgccagga
     6061 atgaatcatc aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa
     6121 cgccagagag tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgcgg
     6181 cctgtaccgc ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca
     6241 cctgtttgat ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac
     6301 cggttacaat cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt
     6361 tgttcagatg ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc aacagctgaa
     6421 tatcgacctg caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct
     6481 tcttcggcat caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac
     6541 gagaagttgt catcacaaag acgttttctt cacgcaagaa attgacggcg ggtagctcgt
     6601 ccggcacacg aataggcata acctgattac ctcactacat acgcttatac gtttagacat
     6661 ccagatagct gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag
     6721 aaagtttcaa ggaaaaggct acgttagaat ataagaatga cgaaaaggag agaaaatggt
     6781 tattagtatt cgccgctcac agcatgagga aggggaggaa ctcgttgcga tttggtgtcg
     6841 atctgtcgat gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct
     6901 ggttcgttcc tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggatcagcc
     6961 ggttgggttt atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt
     7021 gcgcggctgc ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact
     7081 gacaaccaac gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt
     7141 taaggttacg ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctgaatct
     7201 ggcgtatgtg ggggaataag ccgcccctgc cgtgcctgcc caggctggag aagacgcgcc
     7261 agcgtcgcat ccgacctttt tctgcaccag cgcaaaaagg cccagtcttc cgactgggcc
     7321 ttctgcttaa tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc
     7381 atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct
     7441 aaggccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt taatctgtat
     7501 caggctgaaa atctttctct catccgccaa aaca
//
LOCUS       DAFAVF010000180         7447 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFAVF010000180 DAFAVF010000000
VERSION     DAFAVF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.182"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(90..913)
                     /locus_tag="LGK42_003922"
                     /pseudo
     CDS             complement(90..913)
                     /locus_tag="LGK42_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(967..2685)
                     /gene="proS"
                     /locus_tag="LGK42_003923"
     CDS             complement(967..2685)
                     /gene="proS"
                     /locus_tag="LGK42_003923"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK2660106.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2797..3504)
                     /gene="tsaA"
                     /locus_tag="LGK42_003924"
     CDS             complement(2797..3504)
                     /gene="tsaA"
                     /locus_tag="LGK42_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBK2660107.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3501..3905)
                     /gene="rcsF"
                     /locus_tag="LGK42_003925"
     CDS             complement(3501..3905)
                     /gene="rcsF"
                     /locus_tag="LGK42_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBK2660108.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVESVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4023..4838)
                     /gene="metQ"
                     /locus_tag="LGK42_003926"
     CDS             complement(4023..4838)
                     /gene="metQ"
                     /locus_tag="LGK42_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBK2660109.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4878..5531)
                     /gene="metI"
                     /locus_tag="LGK42_003927"
     CDS             complement(4878..5531)
                     /gene="metI"
                     /locus_tag="LGK42_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBK2660110.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5524..6555)
                     /gene="metN"
                     /locus_tag="LGK42_003928"
     CDS             complement(5524..6555)
                     /gene="metN"
                     /locus_tag="LGK42_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBK2660111.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6743..7315
                     /gene="gmhB"
                     /locus_tag="LGK42_003929"
     CDS             6743..7315
                     /gene="gmhB"
                     /locus_tag="LGK42_003929"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBK2660112.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1828 a         1928 c         1889 g         1802 t
ORIGIN      
        1 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
       61 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
      121 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
      181 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
      241 gccagtgtaa gtggatgccc ggctttggca aaggcatccg tgacaaattc cgaacaaaac
      301 cacgactttt tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg
      361 cactttggcg aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa
      421 agggaataaa ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa
      481 acgcggtgat atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct
      541 tatcactatg cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt
      601 ctgcaacgtt attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga
      661 cgcggattcc aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga
      721 gatcaggtgc cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt
      781 tgacagccca tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga
      841 tatcaacggt acaagccgaa agtaaaagaa agctggggag aaacaggcgg cagtacgcct
      901 ttggtttatc cattttatac aatccatgta aaaaaagggc cctgaaattc aggacccttt
      961 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
     1021 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
     1081 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
     1141 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
     1201 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
     1261 ctggaacggc gcgatagcgt caggccatac gatgcctcgt tcgtcgtagt tctgctcaat
     1321 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
     1381 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
     1441 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
     1501 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
     1561 ccagttgatg ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
     1621 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
     1681 cggaccggct ttaaccacgg cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
     1741 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
     1801 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
     1861 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
     1921 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
     1981 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
     2041 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcttg
     2101 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
     2161 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
     2221 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
     2281 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
     2341 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
     2401 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcagctgg
     2461 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
     2521 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
     2581 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
     2641 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
     2701 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaaagtgat ttagtttacc
     2761 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
     2821 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
     2881 ggttttgccc gtttcctcac ctttacgata ggccgggcgt gggtcctgcg ccagtacttc
     2941 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
     3001 ctctgcggta aaactcaccg ccatctctgc cgctggcgca ctttgcgcat agctggcact
     3061 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
     3121 atcgaccaga tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc
     3181 tttcagctct accagcgaca taccaattgg gttagggcgg aaagtagagc gtgttgcgaa
     3241 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
     3301 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaacgctt ccaggccgcg
     3361 aacggcgtcg gcctggttgt agggagcaat gagatgcagt tcgccgttgg cgctttttac
     3421 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
     3481 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
     3541 gcctgacgat agcagcctgg cgtaccgctg gtgacttcgc agctatgcag taataccgca
     3601 ttggccttca ttttcgaggc gttgatttgc atccgcttac gcgcggttgg aatgctcggc
     3661 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
     3721 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
     3781 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacgga ttcgacaggg
     3841 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
     3901 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
     3961 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
     4021 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
     4081 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
     4141 gttacgatca ggtttacgta cggggagtct ttatcttcga caaagatacc gtctttcgct
     4201 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
     4261 tcgtccagag atcgaggcag ctgcggtgct tccagctcaa caattttcag attttttggg
     4321 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
     4381 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
     4441 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
     4501 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
     4561 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
     4621 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
     4681 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
     4741 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
     4801 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
     4861 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
     4921 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta
     4981 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
     5041 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
     5101 ggcaatgctt ccggtaacag cactttacgg acgatctgca tcggcgtggc acccattgca
     5161 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
     5221 ataaacggtg ctgcaccaac ggttaacgga acaatcgctg cctgcaaacc aatcgatgta
     5281 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
     5341 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
     5401 cccgggcgcg taacataaag caggacgccg accggcagac caatcacaaa gccaaaaaat
     5461 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcaa ccacatcatc
     5521 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
     5581 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc
     5641 accggcgtaa tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
     5701 ttcagaaagc agcggggcat cgaccgattg accggtaaac tccaggcgca acatcggcac
     5761 gcagtcagta aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg
     5821 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
     5881 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
     5941 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
     6001 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
     6061 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
     6121 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
     6181 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
     6241 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
     6301 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca gttcctggcc
     6361 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
     6421 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
     6481 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
     6541 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtggc atttcgaatg
     6601 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
     6661 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcacatg
     6721 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
     6781 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
     6841 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
     6901 caaccagtct ggcattgctc gcggtaaatt tactgaagcg cagtttgaaa cgctgaccga
     6961 gtggatggac tggtcgctgg cggaccgcga tgtcgatctg gatggcatct attattgccc
     7021 gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc tgcgactgcc gcaaaccaca
     7081 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
     7141 ggtgggcgat aaattagaag atatgcaggc agcggctgcg gcgaacgtgg gaacaaaagt
     7201 gctggtgcgt acgggtaaac ctattacgcc tgaagcagaa aacgcggcgg attgggtgtt
     7261 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cataatgatt
     7321 aaaagatgag caattgaaat aaaaatgcat ttttccactt gtcttcctga gacgactccc
     7381 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
     7441 ttgaaga
//
LOCUS       DAFAVF010000181         7343 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFAVF010000181 DAFAVF010000000
VERSION     DAFAVF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.183"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(329..1429)
                     /gene="chaA"
                     /locus_tag="LGK42_003930"
     CDS             complement(329..1429)
                     /gene="chaA"
                     /locus_tag="LGK42_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBK2660113.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1699..1929
                     /gene="chaB"
                     /locus_tag="LGK42_003931"
     CDS             1699..1929
                     /gene="chaB"
                     /locus_tag="LGK42_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBK2660114.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2087..2782
                     /gene="chaC"
                     /locus_tag="LGK42_003932"
     CDS             2087..2782
                     /gene="chaC"
                     /locus_tag="LGK42_003932"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBK2660115.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2826..3179)
                     /gene="ychN"
                     /locus_tag="LGK42_003933"
     CDS             complement(2826..3179)
                     /gene="ychN"
                     /locus_tag="LGK42_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBK2660116.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3364..4758
                     /gene="ychO"
                     /locus_tag="LGK42_003934"
     CDS             3364..4758
                     /gene="ychO"
                     /locus_tag="LGK42_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBK2660117.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSLWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4759..5409)
                     /gene="narL"
                     /locus_tag="LGK42_003935"
     CDS             complement(4759..5409)
                     /gene="narL"
                     /locus_tag="LGK42_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBK2660118.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPAITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5402..7198)
                     /gene="narX"
                     /locus_tag="LGK42_003936"
     CDS             complement(5402..7198)
                     /gene="narX"
                     /locus_tag="LGK42_003936"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBK2660119.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         1873 a         1800 c         1871 g         1799 t
ORIGIN      
        1 ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
       61 gggataaagc ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta
      121 cgtctcaatt catcttcact tcatccctga aatgtttgca ataaagagta cattccggct
      181 tttcaacagc tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta
      241 acgagctgtc caggtagtat gaagaaatac aataagcagg aatttatctt atctcccccg
      301 atttccgtca tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa
      361 acagtgccag atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat
      421 ggcacagcac taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgta
      481 attcgttacc cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaatggttg
      541 ctaacactga accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt
      601 ttaacgcacc taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg
      661 cgacaggggc attcatgctg tcgagcaatg tctccagcgg gctggcattc attttggtga
      721 ctgcaataac ggcaatcaga tggataatca accagatagc atgccacacg ctgctatggg
      781 cagacggttt accgtgatgc gggtcatcgt catcactgtc atcttcgtgc tcgtagacaa
      841 acaaactttg atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa
      901 ttaatgctac cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa
      961 ataccagtac gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca
     1021 gattcatata ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccaaccagcc
     1081 caccggtaac aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt
     1141 cgccggttgc cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa
     1201 gaataagcga accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa
     1261 cactaaaggc gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg
     1321 gtagtgtctg gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa
     1381 gcgaagtctc cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc
     1441 ctttgcagat gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc
     1501 tggatatcgg taacatttta cgaattttta ccccactgtc gattttttac tcattgggac
     1561 ataaaaataa gtattacgtt tagacaatgt ttgttttagc gtttcttaag aacagtctga
     1621 catgaaaatt cctatgtttt ggcaagagta gatattgttg accacactta atgttcaact
     1681 ttgtaaaagg agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag
     1741 cacgttctac cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat
     1801 caatataaag ataaagacga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa
     1861 gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa
     1921 aaatcgtaaa accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc
     1981 cgtggattgc atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga
     2041 caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga
     2101 tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg
     2161 gtcagcagag caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg
     2221 accggtgtgg atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga
     2281 gtcgtgcacc ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg
     2341 gcgcgggact gcgcaccagc cgggacggat gcttgcactg aaagagggcg gacgcactac
     2401 aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa
     2461 gcgcgagatg attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg
     2521 atgcacagta aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga
     2581 cactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa
     2641 cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg
     2701 gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt
     2761 gttacggccg ggattcgcct gagtaaactt ccctcatagt ggggcgtcag acgccccctc
     2821 aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag ctccacaaga
     2881 gtaccgattt ccaccccatc aataagagga agtgtgctaa tcccgcgccc gtcggtacag
     2941 gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg
     3001 ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg
     3061 aacagacgca gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg
     3121 ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt
     3181 atttactcct gtattcaggg aattagacat tcatcttcta tcttactgct tctgcagcgt
     3241 ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag
     3301 ttgccagtaa tcgttattct ttaaggctat ggtttttcat tattaccgga agttaccgac
     3361 gttttgagcc gtttcgttcc tcgcattatc ccgttttatt tactcttgct tgtagcaggc
     3421 ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatccctt tgataataac
     3481 aatgatggtc tgccggattt aggcatggct cccgaaaatc atgatgggga aaaacacttt
     3541 gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc
     3601 gagcaggcaa aagctttcgc attgggaaaa gtccgcgacg cgcttagtca acaggttaat
     3661 cagcacgtag agtcctggct atcactgtgg ggaaatgcca gtgttgacgt caaagtggat
     3721 aacgaaggac atttcaccgg cagtcgtgga agctggtttg tgccgttaca agataatgat
     3781 cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggtt ggtgagcaat
     3841 gtgggcgttg ggcaacgctg ggcgcgcggc aactggctgg tgggttataa cactttttat
     3901 gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gtgccgaagc gtggggcgaa
     3961 tatttgcgac tatcggcaaa cttttatcag ccgtttgctg catggcatga acagacagcc
     4021 acgcaggaac aacggatggc gcgcgggtat gacctgacag cccggatgcg catgccgttc
     4081 tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgttgatttg
     4141 tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc
     4201 cctgtgccat tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa
     4261 aataacctcg ggctgaatct taattaccgc tttggtgtac cgctcaaaaa acaactttct
     4321 gcgggtgagg ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga
     4381 aataatctac cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca
     4441 ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt
     4501 tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc
     4561 gcacaagcca acagcgcgga gggctggacg ctgatcatgc ctgactggca gaacggggaa
     4621 ggggcaagca atcactggcg attgtcggtg gttgtggaag ataaccaggg gcagcgtgtc
     4681 tcctccaatg agatcacgct aacgcttgtc gaaccgttcg atgcattgtc aaacgacgaa
     4741 ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg ctgcttccac
     4801 gcgagacttg agcttcattt tcttcagcat gtgcttgacg tgcactttta ctgtgctttc
     4861 ggtgatatcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag
     4921 aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc
     4981 gcgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc
     5041 tgcctgatgc aatgctttca gcagatcttc cggttccata tcttttaaca gatagccatc
     5101 cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac
     5161 aatgcgccct gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc
     5221 gggcatattg agatctaaca ggatcagatc gggatcaaga gattccgcca gttcaatacc
     5281 ctgttcgcca ttactcgctt cgccaaccac ggtgatagct ggtgccatac tgataagctg
     5341 ttttacgcca gttcgcagca tcgggtgatc gtcaatcagc aggatagtag ccggttcctg
     5401 attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca
     5461 ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg
     5521 cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc
     5581 cgcagccgtt atcctgtacg gtcagtttga cctgattatc gttttgcgcc accgtcacca
     5641 cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca
     5701 acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca
     5761 ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc
     5821 caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc gcccaggatg
     5881 cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc
     5941 cctgcatctg taaacaactc acctgcatct tcatacaaga gagagattgg gcaatagaat
     6001 catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt
     6061 cctgatggcg atccaacgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt
     6121 gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact
     6181 gcgtatgaga gtcagccagc cgccacttca gggttgtgcc gcgatcgcca acgggtaata
     6241 cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctggt tggcaggtaa
     6301 actcctgatg attctcttca tcatcagtat cataaacccg cagttcgata tcacgtagca
     6361 gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcttggg
     6421 aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta tgctccagcc
     6481 cggcggtttt ctcctgaacc cgctgctcaa gtacggcata actttcggcc agttctgcag
     6541 acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc
     6601 gttgggtaaa atcgcgatga ctgacggcac tcgccattgc cagcagttgc cgccacggtt
     6661 gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc ataaataccg
     6721 ccattacccg atggaccagt accactgtct cgatgcgcat ttccgtggtg cggtcaaaac
     6781 cagataccag ttgatcaagc ccggcaacaa actggctgac atccgctgac accgtttctc
     6841 ggttttgtgc acgcatcaac gcagggatca gttcattacg ccagtaatct tgtaaaccct
     6901 gtaattgcgc cagttgtccg tctcgttctg ctgctcgagt caactcggcg ctaaatgccg
     6961 tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact gctgccaaca
     7021 gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa
     7081 cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca
     7141 acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt
     7201 aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct
     7261 ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa gagttactcc
     7321 ttatttgccg tgtggttagt cgc
//
LOCUS       DAFAVF010000182         7316 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFAVF010000182 DAFAVF010000000
VERSION     DAFAVF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.184"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            222..1778
                     /gene="yoaE"
                     /locus_tag="LGK42_003937"
     CDS             222..1778
                     /gene="yoaE"
                     /locus_tag="LGK42_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBK2660120.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(1782..3380)
                     /locus_tag="LGK42_003938"
     CDS             complement(1782..3380)
                     /locus_tag="LGK42_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2660121.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(3511..4875)
                     /gene="sdaA"
                     /locus_tag="LGK42_003939"
     CDS             complement(3511..4875)
                     /gene="sdaA"
                     /locus_tag="LGK42_003939"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBK2660122.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(5059..5637)
                     /locus_tag="LGK42_003940"
     CDS             complement(5059..5637)
                     /locus_tag="LGK42_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBK2660123.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(5641..7002)
                     /gene="pabB"
                     /locus_tag="LGK42_003941"
     CDS             complement(5641..7002)
                     /gene="pabB"
                     /locus_tag="LGK42_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBK2660124.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            7076..7255
                     /locus_tag="LGK42_003942"
     CDS             7076..7255
                     /locus_tag="LGK42_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBK2660125.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1763 a         1859 c         1936 g         1758 t
ORIGIN      
        1 tgccaaatca gaggcgtctc tgatatgttt aactcccgtt taacaaccat ggagtatagg
       61 gcagtagccc gcagtatgga tcgtcaccga cgtcatttca gcatcaggcc ttttaacgcc
      121 tacctttctg gctctctatg ccgcaccttt cgtttgcatt ttgtcgttac gcctgcatta
      181 tttctggcgt cgaatagcta ttccttaagc aggagcttgt catggaattc ttaatggacc
      241 cctcaatttg ggcggggcta ctcacgcttg ttgttctcga aattgtgctg ggtatcgata
      301 acctggtctt catcgccatt cttgctgaca aactgccgcc aaaacaacgc gataaagcgc
      361 gtttgctggg gttatcactg gcgctgatta tgcgtctggg gctgctgtcg ctgatttcat
      421 ggatggtcac gctgaccaaa ccgctattta ccgtcatgga tttctccttc tccggacgcg
      481 acctgattat gttgttcggg gggatattct tgctgttcaa agcaacaacc gaactgcatg
      541 aacggctgga aaaccgcgat catgattccg gccacggtaa aggctacgcc agtttctggg
      601 tggtcgtcac acagattgtc atccttgacg ccgtcttctc gttggatgcg gtaattactg
      661 cagtagggat ggttaaccat ctgccggtga tgatggcggc ggtagtgatt gcgatggcgg
      721 ttatgttgct ggcatccaaa ccgctgacgc gattcgttaa ccagcacccc acggtggtgg
      781 tgctctgtct gagcttcctg ttaatgattg gtctgagtct ggtggcagaa ggtttcggtt
      841 tccacattcc gaaaggttac ctgtatgccg cgattggctt ctcgatcatc atcgaagtgt
      901 ttaaccagat tgcgcgtcgc aactttattc gccaccagtc gactttgccg ctgcgagcgc
      961 gtactgccga tgccatcctg cgtttgatgg gcgggaaacg tcaggccaat gttcagcacg
     1021 atgccgataa cccgatgccg atgccgatcc cggaaggtgc atttgccgaa gaagaacgtt
     1081 acatgattaa cggcgtactg acgctggcgt cgcgttctct gcgcgggatc atgacgccgc
     1141 gcggtgaaat aagctgggtt gacgctaatc tcggggtcga tgaaatccgc gagcaactgc
     1201 tctcttcacc gcacagtctg ttcccggtat gtcgcggtga actggatgaa atcatcggta
     1261 ttgtacgtgc taaagaactg ctggtggcgc tggaagaggg cgttgatgtg gcggcgattg
     1321 cttcggcgtc tccggcgatt atcgtcccgg aaaccctcga tccgatcaac ctgttgggcg
     1381 tgctgcgtcg tgctcgcggg agctttgtta tcgtgaccaa cgagtttggt gtggtacaag
     1441 gtctggtcac gccgctggat gtgctggaag ccattgcggg tgaattcccg gacgctgacg
     1501 aaacgccgga aatcattact gatggtgacg gctggctggt aaaaggcggt acagatttgc
     1561 atgccttgca gcaggcgctt gatgttgagc accttgccga tgacgatgat atcgcgacgg
     1621 tcgcgggcct cgtgatctcg gcaaatggtc acattccccg tgtgggcgat gtgattgatg
     1681 tagggccact gcatatcacc atcattgaag ccaatgatta tcgtgttgat ctggttcgca
     1741 ttgttaaaga gcaaccggcg cacgatgaag atgagtaagc attaacgtaa cggcataatg
     1801 ggcgtgatat gtccattatg ccgggcgggc ggcggttggc tgcccgccag ccatttggga
     1861 aaatcacgta gcggcatcgg ctgtgcgtac aaataccctt gcaaaatatg cacgccatga
     1921 cggcgcaaat acttcgcctg ttcttgcgtt tccacgccct ccgccaccag ttcaatattc
     1981 agtctttgcc ccagcgcgat gatgatatcg gtcaccgtcg agttaaccgc gtcagaacct
     2041 atagctgcgg taaatgactt atcaattttc agcacgtcag gacgtaatgt ttcaagccag
     2101 gaaaatgaac tgttgccggt gccgaagtca tcaatcgcca gtttgacgtt tttacgatgt
     2161 agctcgcggg caatccgata atcaacatcc agtaaggcat cgcgttcggt gatttcgagg
     2221 atcagttgct gaatcgggtg agcgctaaac cagtactgat tgagatcttt tatcagcaca
     2281 ccacggcgaa aatggctggg tgcgacgtta atgccaacat gaaactgact actcatcggg
     2341 aaaacatggc gctgacgaat ggtttctgcc atcacatagc gggtcagtgg cacaattaaa
     2401 tgatgttctt ccgcgatagg aataaacaca tccggtgaaa tccagccctg acgcggattg
     2461 ttccagcgca gcaaaatctc tacaccaata cattgctggc tgcgcgcatt aagcaaaggc
     2521 tgacagaaca attcgaactc atgttgcgcc aggcccagat tgatttcgcg ggaaaagctc
     2581 atccggtaag cggtcgccag ccaggcgatg tagcccacca gtaaacttag cagcaccgcc
     2641 agcggtaatt gtgttggaag atagtgccat gccagcgccg tagcccccgg accattaacg
     2701 ttaatggtaa aaggaaagcg ttgcgaagaa acctgatagt tttcattgtc ttcaggttgc
     2761 ggaagggaat ctaccagccc attaccatag agcaaatgcc gtttgtccac cgtcaggctg
     2821 gcgctactga tttgcggcag ttgtggctca aggagcattg ccgtcagtaa gtcgatgtta
     2881 atcatctcca tgaccccagc attgctactg cccgctgcag gcgtccattg aatcaaaacc
     2941 ggactgcctt taatcagggc acggtcgatc gtcaggcgta aaagtggttg cggtgcagga
     3001 agttcagcca gaatgtccac gacgggcaca ttgcgataac caaaaatgct ggagcaatag
     3061 agtgtgccgt cttgcaccag gccaatggat cgcacagttt ggagttttgc cgcctgttta
     3121 cgtaatggca aatgggcgac agagcagggc agaccaatca gcggaagcaa gacttcgcta
     3181 cctgcctgta ggggaagcag tactttatct aattcctcta cagcgtgatt ggcgaattgt
     3241 actacccgtt gttgatttaa gttacgctgt gaaataaatc gcacgctcag ggtcgaagcg
     3301 agcgtaacga gtgcgcaaat cgtacaaaca atcattcgat tacggcgata ggttttaatg
     3361 atccgttgtg ctttttgcat gcgggccacc ctgtaagcgt atggcgacaa acgcctgacg
     3421 caacagtgga agtgtagtgg ggaatattac agcagacgag aaagcgggta taaattcgcc
     3481 catccgttgc agatgggcga gtaagaagta ttagtcacac tggactttga ttgccagacc
     3541 accgcgtgag gtttcgcggt atttggcgtt catgtcctta ccggtttcgt acatcgtttc
     3601 gatgacctta tccagcgaga cgcgcggtgc actggtgcgg cgcagagcca tccgtgcggc
     3661 gttaatcgcc ttcacagagg caatggcatt acgctcaatg cacggcacct gaacctgccc
     3721 tgcaaccggg tcgcaggtta aaccaaggtt gtgttccatg ccaatttccg ccgccacgca
     3781 aacctgttcc gggctaccgc ccagcagttc tgcaagaccc gcagcagcca ttgaacaggc
     3841 aacacccact tcgccctggc aaccaacttc cgcaccggaa atagaggcgt tcattttata
     3901 caatgcacca atcgcgcccg ctgccataaa gtaacgggta tagatgtccg ggctgaccga
     3961 ttcaataaag tggtcatagt aagccagcac tgccggaacg ataccgcagg caccgttggt
     4021 tggcgcagtt accacacgac caccggcggc gttttcttcg ttaactgcca gcgcaaacat
     4081 gtttacccag tcaatgacat tcatcggatc gttagacagt ttatcgctgg aaactaacat
     4141 ccggcgcagg gcagacgcac gacgtggcac gcgcagcggg cctggcagta caccttcggt
     4201 gttcatcccg cgatcgatac atgcctgcat ggtttgccag acatgcgcga aatactcgtc
     4261 gatctctttc ttgctgtgca gcgccagttc gttctgcata gcgagaccag acagcgaata
     4321 gccggtttca ttacagtacg cgagcagttc ggtggcagat ttgaacggat acggcacgct
     4381 cacttcgttg gcagcatcct gaccaaagtg ttcttcatcg acgataaaac cgccgccgat
     4441 ggaataataa gttttgctgt agacgacttc atcgccgtta taggcgtgga tttgcatacc
     4501 gttttcatgc agcggcaggt tgccgttatg aaaacgcatc ccgttgtcgc gcgggaaatc
     4561 cacttcatgc cgtccctgtg ccagcagcag acgttcgcgc tcttctacgt cgcgaataaa
     4621 accgggaata ctgtcgatat ccacggtggc aggttcgtta cctgcaagac ccatgataat
     4681 ggcgatatcg gtgtggtggc ctttacccgt cagcgacagt gaaccataaa cgtccacggc
     4741 aacgcgagta acgctatcca gtaagccttt ttcgaccaga tcatcgacga actgtttacc
     4801 cgccttcata ggccctacgg tatgggaaga tgagggacca atccccacct taaacatgtc
     4861 gaatagacta atcacgataa tactcctgac aaggtgactg gacttccagt aacgaactaa
     4921 taacagcgca tagtgtaaga gggaacgcgg cgactggctt aactattcac atgaattaaa
     4981 ctaatcaaaa accgcgattt aactaatatt gcgatgattg tctcaagtag tgttttgtag
     5041 atgtaaagat aagtatagtc agggtttcac accaatttgc agcgccagct cacgaattat
     5101 gcctgcggtc attccccata caaaatactg ttcgtaccag gacagccata cccgatgtga
     5161 atcgccacgg cggtagatat ctaaagggtg ataacgaccc agatgtaatg cctgggcgag
     5221 cggcatttca aacaccgccg agacttcatc ttcactggcg cgatacggca gatcgggcgg
     5281 gataatgccg accactgggg ttacctgata gccagttacg ctatcgacgg gcggcagcac
     5341 gccgataact tcaacggcgg aaggcggtat agcgacctct tcttcagctt cgcgcagcgc
     5401 ggcggcgata actgatgcgt ccgtgtcatc gactgcacct ccagggaatg ccacttgtcc
     5461 agcgtgttta cgcagatgaa ttgaacgctg agtcagcaac aaccccggtt gcggtcgacg
     5521 gacgatgggg attaacacgg cagcctgacg atgatttagg gtttcccggt taatttgcgg
     5581 gcgcaaaagt tgaaagcgag ataaaaaatc atcaagcgtc aggctacggt attccacgtc
     5641 ttacttctcc agttggcgta atatcttatt aactttatca aaagtttcct gatattccgc
     5701 ttcttcctgg ctatcggcga caattccacc gcccgcagag cagtatattt gtccgttaat
     5761 ggcagtcagc gtgcggatag taatgctggt atccatgttg ccgcaaaagc tcaaatagcc
     5821 aatgctgccg caccaggcat tacgtcgctg cggttccagt tcgtcgataa tttccatagc
     5881 ccgtactttc ggagccccgg ttattgagcc accaggaaaa gctgcgcgca gcagatcgct
     5941 ggcgtgtaac tgttctggta gtcgcgccgt tatagtgctg accagatgat gcacggcagg
     6001 gaagggttcc accacgaaca gctctggtac ttttaccgaa ccggctacgg caacacgacc
     6061 gatatcatta cgcattaaat cgacaatcat cagattttcg gcacgatctt tcgctgagtt
     6121 cgccagtttt tctgcttgtt tgctatcttc ctgaggatcg ggcaggcgtg gtagcgtgcc
     6181 tttaatcggg cgggtctgga tttcactatt atcacaaaga ataaatcgct ctggcgaaag
     6241 gcttaaaatt gcaccctgtt caagacgtaa aaaagcgcta aatggcgcgc ggttggcctg
     6301 attaagctga aggaatgcct gccattcatc gccagaatag gtcgcatgaa aacgctgggc
     6361 gagattcacc tgatagcaat caccgctgtg cagatattcc tgtacctggc gaaatttttc
     6421 gccgtactgc tcgcgggtca tattggattg ccagtcggaa gtgagcgtga aatcttcctg
     6481 cggcgagaat tgctggcttt ccagccaggc ccgacgggca ttgacatcat tatgactcag
     6541 caaagaaact gtatgacgct ggtggtcgac aatgagcgcc caatcgtaga tacccactgc
     6601 catatccggc agaacgatat cttgttccgc aatttctggc agtgactcaa aacggcggcc
     6661 cagatcgtag ccaaacaacc ccagtgcgcc gccctgaaat ggcaaatctt cgttatgcgt
     6721 tgggcgaatg tctgcgcgat ccagcacctg ctggagcacc tgtagcgggt catcagtggt
     6781 cgttgtgcgt ttttcgcttt cactaacaac ggtttcttta ccgaaagtgg ttaaagtgca
     6841 aatcggatcg gcgaccacaa tatcaaagcg gctatacgga tgatcggcat agccggagtg
     6901 taaaagcatc gcccacggca ggtggcttaa gcgggagaaa taaaattcag cggcgtcctg
     6961 acgccagggt aaagtaatca cagcgggaga taacgtcttc atcagtcctg actctactgg
     7021 ctatgtgccg ggtagcatga aataattagc gccaacaatt tagcaggagt taacaatgtt
     7081 tgcaggttta ccttcactca cccatgaaca gcagcaaaaa gctgtcgagc ggatccagga
     7141 actgatggca caagggatga gcagcggcca ggcaatcgcg ctggtggcag aagagctgcg
     7201 tgccaaccac tccggcgagc gaatcgtggc gcgttttgag gatgaagacg agtaacacac
     7261 taatccgtat cgatttcgtt atagaatccg atgattattt gattgtgaga aaaagg
//
LOCUS       DAFAVF010000183         7266 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFAVF010000183 DAFAVF010000000
VERSION     DAFAVF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.185"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..625)
                     /gene="wcaD"
                     /locus_tag="LGK42_003943"
     CDS             complement(<1..625)
                     /gene="wcaD"
                     /locus_tag="LGK42_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBK2660126.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAG"
     gene            complement(600..1817)
                     /gene="wcaC"
                     /locus_tag="LGK42_003944"
     CDS             complement(600..1817)
                     /gene="wcaC"
                     /locus_tag="LGK42_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBK2660127.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVCFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQECP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGLGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1814..2302)
                     /gene="wcaB"
                     /locus_tag="LGK42_003945"
     CDS             complement(1814..2302)
                     /gene="wcaB"
                     /locus_tag="LGK42_003945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBK2660128.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2305..3144)
                     /gene="wcaA"
                     /locus_tag="LGK42_003946"
     CDS             complement(2305..3144)
                     /gene="wcaA"
                     /locus_tag="LGK42_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBK2660129.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     NSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3237..5399)
                     /gene="wzc"
                     /locus_tag="LGK42_003947"
     CDS             complement(3237..5399)
                     /gene="wzc"
                     /locus_tag="LGK42_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBK2660130.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5402..5845)
                     /gene="wzb"
                     /locus_tag="LGK42_003948"
     CDS             complement(5402..5845)
                     /gene="wzb"
                     /locus_tag="LGK42_003948"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBK2660131.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5851..6990)
                     /gene="wza"
                     /locus_tag="LGK42_003949"
     CDS             complement(5851..6990)
                     /gene="wza"
                     /locus_tag="LGK42_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBK2660132.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         1664 a         1940 c         1837 g         1825 t
ORIGIN      
        1 tccctgcgag aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga
       61 gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc
      121 gcttgccgcc gaatttaatg aaattcagta catagctgtt gctataaatc agatatttcg
      181 aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg
      241 ccagcgccac caccagcccg accaccagat aaaagaaacg taatatcttg cgatggttgt
      301 gtggcgaaat cgttttaaag cgaatactcc acaccatgcc aataatgatc acaatataga
      361 caaacagcat ggttgaagtg acgtatttac tggcatccaa cgactgacca aacagatagt
      421 tgaacgccgt gagtcccgca ccaatcccta aggcaatcat caatttttta acgctgatgc
      481 gctctaaaaa cagcagtaac aagacaggta aaaaagtgac gatagtgatg gggaaacttt
      541 cgccaagctg ggcgattttg acgttaacca gcaggtagat cagcggcagc aggaggtagc
      601 tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg
      661 cggctgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt
      721 ttgcttaact gcaccagttg cagcacgtct tcttcgctga cagttttacc gccggatttt
      781 tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacacc aatcgatagc
      841 gcctcacaca aaatcagcgg gtagttatcg acgcgagaac tgaacaccag cgcatccatc
      901 tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg
      961 ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc
     1021 atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc
     1081 accgcgattt tcggcttacc ctgggtttcg cgcaccggag gcagatccgc cagaatcgct
     1141 tctgttgcca tatcaatgcc attattgata atccggcaac gccctagacc gtacaggcta
     1201 ttgaaagcat cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc
     1261 tcacgaaata actggcgttt gcccgccacc agttggtgtg cgcgatcaat cttcaccggc
     1321 ggataattat ttaaggtcgg gcattcctgg cagcccgttt tccagccttc gcaaccgtcg
     1381 gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac cagagtgacg
     1441 tccggtttat ggtttttcac tttttcgcaa aagcacacca cgctcttaag atttagccag
     1501 tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga
     1561 tataactcat tgaaattgcc aaacagatcg cgattaaaca gacgaaacag ggcaatattc
     1621 gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgacg
     1681 ctctctttgc cgcctttgcc gtaaccgtag acaaaatgtg acgccagccc ctgttgcagc
     1741 gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta
     1801 aattgcagaa tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt
     1861 tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg
     1921 tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac
     1981 atgccatgtt atcggcacca cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat
     2041 cccccgctac cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc
     2101 ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat
     2161 acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg
     2221 aacaaaaatg agcaacacga taggcaagaa ccatgcagca cgggcgtaaa ctccagctgt
     2281 tggcacgcag atcttccagc atctttagcg cccccgaata ccatcagcca gacgtttgcc
     2341 gttacgcacc gacagcagcg tcagcaaagt gcgccaggtc atacgtttat tgcggatctg
     2401 gtagagggta aacagctgat attttttgct tgcacggtca aatttatctt tgtgcttgcg
     2461 gtaaaagtgg aagtacccgg agaatttttt cggtgacgag gtgatctgca tttcaccgtg
     2521 attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccat actccaccac
     2581 catccgcagg aagatgtcgt aatcctgtgc tgctttcagt tcggtatcga acaagcactc
     2641 tttgaaacgc cacgcccagg taaaaacctg attgccaatg atattgcgtt tatagaacag
     2701 gcggcgtgag tacggcgatt tgggatacag tggcaaactt gctggttggg aatagacttc
     2761 gccctggcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt gctgtttatg
     2821 ggcgaggaag acgctcagac ggttgggtgt ccattcatca tcatcatcga tcccggtgat
     2881 atattcccct tgcgccagca taatcgcctg gttacgtacc gcgcacgccc cgctgttaat
     2941 gtcgttgtga atgtaggtaa tacgcggatc gttgagggcg gtgacgtact gttgcagttg
     3001 ttcccaggag ttggagcaat catccacgat gatcatctcc cagttgctgt agtcctggcg
     3061 caagaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggtcg gcatatagat
     3121 tgagatcagc ggattgtctt tcataggttg ttctcccggc tcatgagctg gtttgcttgc
     3181 cggatgcggc gtgaacgcct tatccggcct acggggcggt gcgaatgcag gcgtcgttat
     3241 ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc gctggcgcgg
     3301 cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc aaagcggctc
     3361 agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat taatgtggtt
     3421 ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc
     3481 aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg ttcgctcatc
     3541 aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa tttggcaata
     3601 gaggtcggtt tagctgcggt agtaatatcg ccctgaccaa tcagaatttc cgacaggcca
     3661 ttgacgttat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc
     3721 aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca gacaaaggtt
     3781 ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc ctgcatcatc
     3841 gcgaagtgca gactggtacg cagactgcgg atggcttcaa tcgccagatc ggttggattc
     3901 cccaccgcca gtagctggct ctgtttatag cgtttaatcc ctttgatggt tttgacgcta
     3961 tcgcgcgctt tctgccattc cgacaacggg atgctggcat agacgctgat accgtgttct
     4021 tccagcacct gcgggctttc gatgccgcga ttaaacaacg agcgcagcag cacacccacg
     4081 atagagagca tcaggccaag aataatcgcc ccaaggataa tcagcccttt cttcggtttt
     4141 agcacaccag gctgagtgat tgccgggtca acaatgcgca catcgccgac ggtgctggct
     4201 tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg ctgaccagac
     4261 tcgacatcgc gggtcagacg gacaatctcc tgctgggttt tcggcatcgc cgttacgcga
     4321 ccgttaagtt tggctttttc gtcttccagc gcctgacgtt tctccagcag cgtgcggtac
     4381 gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt cagttcgttc
     4441 aactgggcgt cgatgttcac catcgaatcg agcaccgctt tcgcttccag cggcagatca
     4501 acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc aaggcggcta
     4561 cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg
     4621 cgctcaatat tttgttcctg atagttacgg gcgatgctgt taagaatgtc gcggatctgt
     4681 tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc gttctccgtt
     4741 accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt ggtgacggta
     4801 aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc ttcttttttc
     4861 agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag ggtgtagttt
     4921 ttatcgtcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg gcggttaaag
     4981 gtggtcactt tcaccgtctc gttctgacgt cccatcaaac gatcccagcc cgcaccgaaa
     5041 atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac cgttttacca
     5101 agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg tttgttggct
     5161 aatgccgaac caatgtcctg cactaacgaa ttgccgctgt tttgctcgat ttgtaccagt
     5221 gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca gagggcaaat
     5281 acggcggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac caggcgacca
     5341 atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt ttctgtcatt
     5401 cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt tcaagtaatg
     5461 tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt tcacattcgt
     5521 tatcccagtg accaaacagc atcactttgc cgcgcatctc gggtgccatc tcgcataagc
     5581 gttcgatatg gcgcttttcc atggtcaaaa tcaggtcata gttgcgacac aggcggcggc
     5641 tgatttgccg tgcacagtga ccttccagag acagttgatg ttctgcggcg acgctgatag
     5701 cggtagggtc agcgccctta ccgaccagcg cgccgagtcc agcggactcc actttcagct
     5761 ccgggtgata acgttgcagt aagcgctccg ccgtcgggga acggcaaata ttgccgacac
     5821 agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc tggctgtatc
     5881 cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac ggttccagcg
     5941 ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga attctgtcgc
     6001 catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa tcttgccgtt
     6061 acgcccgccc tcgcctttca acggacgaat gacaaagatg ccgctggcgt tggaggtggt
     6121 catgtcgatg ccttcggcat tgcccagagc ttcagtcagg gtcatgccgc taaagtccat
     6181 tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat catcattacg
     6241 cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt cgccgttttg
     6301 catcagcgcc tgcaaagaaa tgcgttcttc acgaccattg tgtgttagca ccacgttgcg
     6361 ccagtcagcg gtgtcggtca gaccacctgc ggcgttgatg gcgtcgagaa tggtcagtgg
     6421 tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat aggccttttg
     6481 cgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg tcgctaagcg
     6541 cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgga ccttgccgat
     6601 atacgggtaa aacattgtgc cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg
     6661 gtactgaccg gctggcgtgg tgagttccgg gtgatcccag acggtgacat taagaacgtc
     6721 ccccggcccg acgcgatact ggtaattcgc gatctcactt tccagcgtca tattggggcg
     6781 cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg gataaacatt
     6841 caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt tgcccatcgt
     6901 cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg acaccaataa
     6961 tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc agagtaatta
     7021 tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt aattgaataa
     7081 aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat tgacagaata
     7141 attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt tagctaccaa
     7201 tacactgatt tagtttaatt tttcacaccc tctcagcatg cagtcgttga tgagaaaggg
     7261 ttatta
//
LOCUS       DAFAVF010000184         7238 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFAVF010000184 DAFAVF010000000
VERSION     DAFAVF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.186"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(19..2802)
                     /locus_tag="LGK42_003950"
     CDS             complement(19..2802)
                     /locus_tag="LGK42_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK2660133.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNIQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2859..5231)
                     /locus_tag="LGK42_003951"
     CDS             complement(2859..5231)
                     /locus_tag="LGK42_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBK2660134.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYDGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5269..6954)
                     /locus_tag="LGK42_003952"
     CDS             complement(5269..6954)
                     /locus_tag="LGK42_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBK2660135.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPAGSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            7157..>7238
                     /locus_tag="LGK42_003953"
     CDS             7157..>7238
                     /locus_tag="LGK42_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660136.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKE"
BASE COUNT         1891 a         1641 c         1631 g         2075 t
ORIGIN      
        1 gtgttgacgg ggctgttatt attttggcaa taatactccg gtataagtat ttaccggatg
       61 agaaagatat tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaacaattg
      121 ctcctgctca gtccatgctg caggatcgtc atattgaata agactattta caatagtgtt
      181 cgctaattgt tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta
      241 ttcattcagt tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc
      301 taacgttaac agttcatcat gtcgttctgg ttgacaagta aaagccagca aatgactgat
      361 atctttggcc tgaggatcaa ccgagaggcg agaagaaacg ctgtatgctc cagatgcctg
      421 ttcacgaata ttaacacgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat
      481 acgcgtcgcc agattaacag gtgtccggga atcataacgc ttccactgtg aaacctgtgc
      541 cacaggttca ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg
      601 tttacctgcg gctaatggcg aatcagagtg tttgattgat cctaagtaac gcgtaattaa
      661 cgccacgagt ttgtcttctg agacattacc gacaatgaca aacgtgatat ccgctggaga
      721 tgaaaacaac tggcgatcgg cagccagcgc atctgcggca gtaaactgta caatctgatt
      781 ttcttgcagt aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa
      841 tttctccgct ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga
      901 tgcccaaata ttgtcgttaa tcgtgctgtg ggtgattcgc tggttaatca actgaaaacc
      961 aggttcaggg ttattagtcc gcgcgctaac ggagagcaac gtattcatgc cactgacttt
     1021 actgctcatg gttaccgaat tttccgcact ccagcgtttc aagctggaag aggagagctc
     1081 accaacgccg cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc
     1141 agggaaactt aaatcgcctt tattcgatac ggcagtaatt tgtagctttt gctcttcacc
     1201 cgcggatttt gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc
     1261 agccagcgtt tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa
     1321 atttctgcct gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc
     1381 caggatagcc gcaggagaca atgctttttt ggcggcaagc tcattgttta ccatttgctc
     1441 ccaaaatgcg tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt
     1501 aatttgctgc cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt
     1561 attatttaat gagctggatg ccaggcgact ggtcagcata cgtaaatcac gttcagcttg
     1621 ctgatcaacc gcatttttca gccaggtgag gcgggtagat ttgacatcat cgagttcttc
     1681 agcagaaaaa ccatgctgat caatggttgc caactctgcc attaatgcat tcgcagcatc
     1741 ctgtatatta tcgtctcgtg catttacacg gaaaaacagc gactgataat cgggtgcaat
     1801 tttaacgctg cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc
     1861 ctgcagacgt tgattgaaca gctgaactaa catgctccat tcagcttgtt cgacaaagct
     1921 ttgctcatcg ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcacccg
     1981 gttttcttta tcattaatga tattaaagcg caggtggttt tcggcttttg tcggccagac
     2041 gcgattttca gctgctttgt tagccggaag cttacttaaa ttatccttta tcagcgccag
     2101 cgcttcttta ctgtcgatat cgccgaccac gataaaggtc atattatttg gttgatacca
     2161 gcgttgataa aattggcgca attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc
     2221 gataggttca cggtctaaat tacgggtatt agccagcagg aaaggacggc gcgcctgaga
     2281 ggtgcgccat ttcgcatcct gatgggcacg ccattcctca gtaattacgc cacgttcagc
     2341 gtctacttcg agtttttcaa aggttgcggc attactccat tcactgaaga ttgccatcac
     2401 ttgttgcaga ttttgtttct gcgtagtcgg caaactcacc tgatacaccg tttcgtcata
     2461 gctggtatag gcattaacat cgcgaccaaa acgcaggccc attgactcaa atgtttcgat
     2521 gactttatta cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc
     2581 cacgccgcgc tcattgtctt cttcctgcaa tgaaccggta tgaatttgca gccataaatt
     2641 tacctgatcc tttggatgag catgcggata aatcatatat cgcaagccat tgcccagttg
     2701 cccggtaatt aacttttcat cctgcggtaa ggcggcggca atcagccgcc cggggagcaa
     2761 cagagttgcc actaacgtca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa
     2821 accgggcgaa tttacccggt taagtaaaat ccgaactatt aaaatttcat gctgacatca
     2881 agccagaaag tacggccact ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg
     2941 gttttgctat ccaatacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga
     3001 aattgtggcg tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttttcgtca
     3061 acatactgct tgtaatctga atattcgctg atgtattgcg catttgtctt acccaggata
     3121 atgcgagctt tacgcgcttc ttgccaggcc agcgtattcg cccacaccaa cccgctcggt
     3181 tgatgcgtga aatccatgtt taaggagatc tttaatgggt tattaaaatc tgccactgga
     3241 acgctatcgt aagaaaccag attaccgtca taaaccacct ggttatcacc ggtattgctc
     3301 tcctcataac cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt
     3361 tgtgggttaa tatctacctg gcggatatgc aggggttcgg ccagctcaaa actgaggttg
     3421 aacgaatgcg ttttggtttt gccatcgttg ttatattcag taatggtggt tttagtcgcg
     3481 ctgtcggtac gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgca
     3541 cgcgcaataa cgttcttacc gattttctgc tgcaatccca ttgccagttc atcgttataa
     3601 ggcgttttca aatcctgata acgcgtcagg gttttattgc ctgataccga ttccgtccag
     3661 ctattgcgga tatcacgtaa tcccatatca agaatattcc caccatagta acggttataa
     3721 cctgcggtaa tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg
     3781 gagatattgt ggtttgacag atagttgtca tagtcatacc gcacgccggg cattaatgac
     3841 acattacgcc agctaatgcg atccgccata tacagtgtgt agttgtcaat tcccaggctg
     3901 cctttacctt tatggtaaat ggtatggtta gttttctttc cggcagcatt aatcacatag
     3961 gattcagact ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag
     4021 tagggttgat gcgaaacatt acccacggcg aatttttgcc agtccaggcg tgttttgaag
     4081 gtgtaattat ctacagcctg ggaaatgtgt cctaatccgc cacgggtgca acgccctgta
     4141 atatcaccat atgtacatga aagttcggtg taccaggtgt catgatcgtg acgggtataa
     4201 tcactaatat gatcccaacc aacggtggta cgaagtttgg cccatgcgag ctgcgtatcc
     4261 atatcccatg ccagaccata ggatttatta cccatttcgc gatcagactg cgggaaggtg
     4321 ctggtattat aatcacggct ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg
     4381 tcgctggcaa accaggtaaa tttgctcaat gcagtatcga taacgttttt atactgtgcc
     4441 cgaccggcga caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg
     4501 cgcgataaac cggcggtaac gccaaagtta tcagcgagtt cctgattaaa cgacaaggta
     4561 taaaagttct ttttaaaatc tggagagtaa taagtacttc ctgaagaacc ttgattaaat
     4621 gcgctcttgt tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa
     4681 cccagtttca cgctgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg
     4741 ccattgaagc gaccgaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt
     4801 aagctgacat caagataata cccctgtgac atcccgctaa tattggttgc actactggca
     4861 tcggactcat tcgctgggtt caggttatta gtggcactaa taccatcaat caaataggca
     4921 ttctggtagg gcgacgcacc gtgaatagag attttctccg ggcgaatatc tccctggttc
     4981 aacgaggtac tttgcgttga atccatgcgt accgcagggt tggttctcag cagatcgctg
     5041 atattgccat tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg
     5101 tttacgggta cagggacagg agcatagacc gtcatctctt caaaaacatg catgtacatg
     5161 tttttgtcat cgacggcctg cgccacatca gcgccacata aaatgacgcc aggaataaga
     5221 actcgcttca tttatttata tctccgtaca gcagggcttt tattgttttt ataaaaccgc
     5281 gctaatgtca caaatatcat cggccaggtt ccagacaccg ggttgatgtg taaccataat
     5341 gacgccgctt gtgggtagtt tttcacgcac taaacgcagt aagcggatag cctcttgttc
     5401 ctcaagatga gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg
     5461 tgccagggcg atacgttgtt tttcgccgct ggaaagaata tctccccagc gatcgtggtc
     5521 atgaatacgc gcagccaatt tccccagacc aacctgatgc agtacttcgc tcaacgattt
     5581 atcgtctacg ggcaggggaa gtgctttaca aataatctct ttcagtaagc cgcttttgat
     5641 taacggtgtt tgtgacacat accaactgcc agcaggagaa gaaatatcac ctttaaacca
     5701 cggccagcag tgggataatg ttttaagcag tgtggttttt cccgcaccag agtagccttt
     5761 cagtaatagc catttgcctg gcgaaacatg aaagttcaag ttctctaata tgatcttatt
     5821 atcaggcgta cgaatactcg cattagccac ttgtaccgca tgttggcaat ttttaggctt
     5881 attcgtaggg cgctgttcag tgagttgatg gaactcatac aagcgatcga taaccgcagc
     5941 cagttcagca agttcgtcat atttataaat aaaccagctt aaattgttcg atactagcat
     6001 aaatgcctgg cgcgatttca tcagtccgcc cagattgatc tgcccgctaa taaactgcgg
     6061 caataacaga aagtagggaa gaacgctaag cgagcgcgaa taaatatttt gccagtaatc
     6121 aagccaccgc tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc
     6181 gcgcaactcc tggcgttgta gagactcagc attgcttagc gcaatcagtt ctgcctgctt
     6241 attatgctgc acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag
     6301 cggacgaata cgtttaccca ccttatgggt aaataaagtt ccaccgatca caatgagcac
     6361 gacggtatag accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag
     6421 cgtacccgcg ctttgccaaa gaataacggt aaaggtgatc agcatgctaa gtgactggat
     6481 gaagccaaat gaaagactga gcgttttgct gatcagtaag agaatgtctt cagcgatacg
     6541 ttggtcaggg ttatctgtat ttttatgctc gccgtagatc tgcgtgaagt agtagttttt
     6601 atctgcaaac cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag
     6661 tagtttaatt aaccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg
     6721 gaaccaaagg acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa
     6781 atcattattc cagtcgttta accagacctg aattttaacc acgccaagga tcattgcgag
     6841 gataataatg attaacaaca ctgaagtttt attattctta cgcagccaaa agggcttcag
     6901 cagacagaga tactttgcta tcaacataca aaacgtaatg ggaatggtta tcattagcga
     6961 aaattgattg tgccatccaa tgattatcta tggcaatgat ttactgatga aatttattta
     7021 agtatcactt aacaatatga aatataaaga aatatttcat tttcatcggt tatggttatg
     7081 ctattgataa tcaagggtat tgctaatgcc ctgacgcata ccacgtaagc caggattttc
     7141 gcaagggaag atgatgatga cgaaacaccc gacaggaatt tatgtcgggt gccttgttaa
     7201 ggtcataaga aggaggctaa gaatggagtt aaaagaga
//
LOCUS       DAFAVF010000185         7192 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFAVF010000185 DAFAVF010000000
VERSION     DAFAVF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.187"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            104..319
                     /gene="ydgT"
                     /locus_tag="LGK42_003954"
     CDS             104..319
                     /gene="ydgT"
                     /locus_tag="LGK42_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBK2660137.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            405..845
                     /locus_tag="LGK42_003955"
     CDS             405..845
                     /locus_tag="LGK42_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBK2660138.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            922..1503
                     /gene="rsxA"
                     /locus_tag="LGK42_003956"
     CDS             922..1503
                     /gene="rsxA"
                     /locus_tag="LGK42_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBK2660139.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1503..2081
                     /gene="rsxB"
                     /locus_tag="LGK42_003957"
     CDS             1503..2081
                     /gene="rsxB"
                     /locus_tag="LGK42_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBK2660140.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            2074..4200
                     /gene="rsxC"
                     /locus_tag="LGK42_003958"
     CDS             2074..4200
                     /gene="rsxC"
                     /locus_tag="LGK42_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBK2660141.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            4201..5259
                     /gene="rsxD"
                     /locus_tag="LGK42_003959"
     CDS             4201..5259
                     /gene="rsxD"
                     /locus_tag="LGK42_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBK2660142.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5263..5883
                     /gene="rsxG"
                     /locus_tag="LGK42_003960"
     CDS             5263..5883
                     /gene="rsxG"
                     /locus_tag="LGK42_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBK2660143.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            5887..6582
                     /gene="rsxE"
                     /locus_tag="LGK42_003961"
     CDS             5887..6582
                     /gene="rsxE"
                     /locus_tag="LGK42_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBK2660144.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            6582..>7192
                     /gene="nth"
                     /locus_tag="LGK42_003962"
     CDS             6582..>7192
                     /gene="nth"
                     /locus_tag="LGK42_003962"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK2660145.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLC"
BASE COUNT         1647 a         1844 c         1919 g         1782 t
ORIGIN      
        1 cgcattctta ttattgtaat aacacaagcg ttcacttttg gtgaaatttt atcatcacta
       61 ataataccta cccctttggt attaattatt ttatggacct tttatgactg ttcaggacta
      121 cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct
      181 taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg
      241 tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt
      301 ctggcactat gtccaataaa gaaagtagcg atcggttata aaagccttat aataacgccc
      361 ctaaaattag atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc
      421 cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttga
      481 gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa
      541 cgctcggcgg acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta
      601 tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg
      661 ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcagta aaagcattcg
      721 ctttttcacc cgtggaagat ggcatagccg ttcgccagtt gctgtttact ttgttggcaa
      781 ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc
      841 cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat aataggcggc
      901 ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg
      961 gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa
     1021 aaactggaaa ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctggcgtct
     1081 atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc
     1141 accctggcat ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc
     1201 aaaaccagcc cggtgcttta ccgtctgctg gggatttttt tgccgcttat caccaccaac
     1261 tgtgcagtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg
     1321 gcgctgtacg gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc
     1381 atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg
     1441 ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg
     1501 taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg
     1561 ccattctggg ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa
     1621 ttgacgaaat cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct
     1681 acgcggaagc catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag
     1741 ctgtgatgct aaaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag
     1801 cgcaagagct aacgcctgcg cggatggtgg cggttattga tgaaaataac tgtattggct
     1861 gcactaaatg tattcaggct tgtccggtag acgccatcgt tggcgctacc cgtgccatgc
     1921 atacggtaat gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc
     1981 actgcatctc gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca
     2041 ccattcccgt gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga
     2101 aaaaataaaa tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc
     2161 aacggtacac ccctgcgcca ggtaccactg gcgcagcgtt ttgttattcc actgaaacag
     2221 catattggcg ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag
     2281 ccgcttaccc gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt
     2341 acggctattg cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt
     2401 attgatgccg atggtgaaga ctgctggatc ccgcgcgacg gctgggccga ttatcgcact
     2461 cgcagtcgcg aagagttaat cgagcgcata catcagtttg gtgtagccgg gctgggcggt
     2521 gcaggattcc cgacaggcgt taaattgcag ggtggcggag ataagattga aacgttgatt
     2581 atcaacgcgg ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgc
     2641 gcggctcagg tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt
     2701 cttatcggca ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg
     2761 gactctaacg atatttctct gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa
     2821 caattaacct acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc
     2881 ggcgtattaa tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc
     2941 gagccgatta ccgagcgtgt tgtaaccctg actggcgaag caatcgctcg cccgggcaac
     3001 gtctgggcac ggctggggac gccagtgcgt catttattga atgatgccgg attctgcccc
     3061 tctgccgatc aaatggtgat tatgggtggc ccgctaatgg gctttacctt gccatggctg
     3121 gatgtcccgg tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgaacttggc
     3181 gaaccacagg aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg
     3241 gatcttttgc cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc
     3301 acgcataaca ttgctgattg cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat
     3361 attcccctgg tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa
     3421 gaaaagcgcg ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc
     3481 gaaaaagcgg ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat
     3541 aaagatgcga ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag
     3601 cctattgtga ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa
     3661 gcccgtaaag cgcaagccag agcgaaacag gcagaactgc agcaaactaa cgacgcagca
     3721 accgttgctg atccacgtaa aactgccgtt gaagcagcta tcgcccgcgc caaagcgcgc
     3781 aagctggaac agcaacaggc taatgcggaa ccagaagaac aggtcgatcc gcgcaaagcc
     3841 gccgtcgaag ccgctattgc ccgtgccaaa gcgcgcaagc tggaacagca acagtctaat
     3901 gcggaaccag aagaacaggt tgatccgcgc aaagccgccg tcgaagccgc tattgcccga
     3961 gccaaagcgc gcaaactgga acagcaacag gctaatgcgg aaccagaaga acaggttgat
     4021 ccgcgcaaag ccgccgtcga agccgctatt gcccgtgcca aagcgcgcaa gctggaacag
     4081 caacagacta acgcagaacc agaagaacag gttgatccgc gcaaagcggc agttgccgcg
     4141 gctattgccc gcgctcaggc caaaaaagcc gcccagcaga aggttgtaaa cgaggactaa
     4201 atggtattca gaatagctag ctccccttat acccataacc agcgccagac atcgcgcatt
     4261 atgctgttgg tgttgctcgc agccgtgcca ggaatcgcag cgcaactgtg gttttttggt
     4321 tggggtactc tcgttcagat cctgttggca tcggttagtg ctctgttagc cgaagctctc
     4381 gtactcaaac tacgcaagca gtcggtagcc gcaacgttga aagataactc agcattgctg
     4441 acaggcttat tgctggcggt aagtattccc cccctcgcgc catggtggat ggtcgtgctg
     4501 ggtacggtgt ttgcggtgat catcgctaaa cagttgtatg gcggtctggg gcaaaacccg
     4561 tttaatccgg caatgattgg ttatgtggtc ttactgatct ccttcccggt gcagatgacc
     4621 agctggttac cgccacatga aattgcggtc aacatccctg gttttatcga cgccatacag
     4681 gttattttca gcggacatac cgccagtggt ggtgatatga acacactacg cttaggtatt
     4741 gatggcatta gtcaggcgac accgctggat acatttaaaa cctctgtccg tgccggtcat
     4801 tcggttgaac agattatgca atatccgatc tacagcggta ttctggcggg cgctggttgg
     4861 caatgggtaa atctcgcctg gctggctggc ggcctgtggt tgctatggca gaaagcgatt
     4921 cgctggcata ttcccctcag cttcttagta acgctggcgt tatgcgcaac gttgggctgg
     4981 ttgttctcac cagaaacact ggcagcaccg caaattcatc tgctgtctgg tgcgaccatg
     5041 ctcggcgcat tctttatttt gactgacccg gttaccgctt ctacgaccaa tcgtggtcgt
     5101 cttattttcg gcgcgctggc gggcttatta gtctggttga tccgcagttt cggcggctat
     5161 cctgacggcg tggcttttgc cgtcctgctg gcgaacatca cggttcctct gatcgattac
     5221 tacacgcgtc cgcgcgtcta cggccatcgc aaagggtaaa ccatgctgaa aactatccga
     5281 aaacacggca ttacgttagc gctatttgca gcgggttcaa cagggttaac tgcggccatc
     5341 aaccagatga ccaaaacgac gattgctgaa caggccagtc tgcaacaaga ggcattattt
     5401 gatcaggtgc tgccagccga acgctataac aatgcgctgg cacagagttg ctatctggta
     5461 actgcgccag agttaggtaa aggtgagcat cgggtttaca tcgccaaaca ggatgacaaa
     5521 ccggtagccg ccgttctgga agcaaccgcg ccagatggct attccggtgc gattcagctg
     5581 ctggtgggag ccgattttaa cggcacggta cttggcacgc gcgtgacaga gcaccacgaa
     5641 acgccagggc ttggcgataa aatcgaactg cgcctttctg actggatcac ccattttgcg
     5701 ggtaaaaaaa tcagtggtgc ggatgatgcg cactgggcgg tgaagaaaga tggtggtgat
     5761 ttcgaccagt tcaccggcgc gacgattact ccccgcgcgg tggttaatgc ggtaaaacgc
     5821 gccggattgt acgctcagac gttaccggca caactttctc aacttcctgc ctgtggagaa
     5881 taaagcgtga gcgaaattaa agacgttatt gttcaggggt tgtggaaaaa caactctgcg
     5941 ctggtccagt tgctcggcct ttgtcctctg ttggcggtca cgtccactgc cactaacgct
     6001 ctgggtttag gacttgcgac tacgctggta ctgacgctga ccaacctgac catttcgacg
     6061 ctgcgtcact ggacgccagc cgagatccgc attcccattt acgtgatgat catcgcctcg
     6121 gtggtcagcg ctgtacagat gctgatcaac gcctacgcct ttggcctgta tcaatcatta
     6181 gggattttta ttccgctgat tgtcactaac tgtatcgttg tgggccgcgc tgaagccttc
     6241 gccgccaaaa aaggtccggc gctttcggca ctggacggct tttcaattgg tatgggcgca
     6301 acctgcgcca tgttcgtgct gggttcacta cgcgaaatta tcggcaatgg cacattgttt
     6361 gacggtgcag atgcgctgtt aggtagctgg gcaaaagtat tacgcgtgga aattttccac
     6421 accgactccc ctttcctgct ggcgatgctg cctccaggtg catttattgg cctgggactg
     6481 atgctggcag gaaaatacct gattgatgaa agaatgaaaa agcgccgtgc tgaagcaact
     6541 gccgaacgtg cattgccaaa cggtgaaaca gggaatgtct gatgaacaaa gcaaaacgcc
     6601 tggagatcct cactcgcctg cgtgagaaca atcctcatcc caccaccgag cttaatttca
     6661 gttcgccttt tgaattgctg attgccgtac tgctttccgc tcaggcgacc gatgtcagtg
     6721 ttaataaggc gacggcgaaa ctctacccgg tggcgaatac gcctgcagcg atgcttgaac
     6781 tgggcgttga aggggtgaaa acctatatca aaacgattgg gctttataac agcaaagcag
     6841 aaaatatcat caaaacctgc cgtatcttgc tggagcagca taatggcgag gttccggaag
     6901 atcgtgctgc gcttgaagcc ctgcccggcg taggtcgtaa aacagccaac gtcgtattaa
     6961 acactgcatt cggctggccg actattgctg tcgacacgca cattttccgc gtttgtaatc
     7021 gtactcaatt tgcgccgggg aaaaacgtcg aacaggtaga agaaaagcta ctgaaagtgg
     7081 ttccggcaga gtttaaagtc gactgccacc attggttgat cctgcacggg cgttatacct
     7141 gcattgcccg caagccccgc tgtggctctt gtattattga agatctttgt ga
//
LOCUS       DAFAVF010000186         7148 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFAVF010000186 DAFAVF010000000
VERSION     DAFAVF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.188"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            99..1433
                     /gene="cadB"
                     /locus_tag="LGK42_003963"
     CDS             99..1433
                     /gene="cadB"
                     /locus_tag="LGK42_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBK2660146.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1513..3660
                     /gene="cadA"
                     /locus_tag="LGK42_003964"
     CDS             1513..3660
                     /gene="cadA"
                     /locus_tag="LGK42_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBK2660147.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3719..5176
                     /gene="dtpC"
                     /locus_tag="LGK42_003965"
     CDS             3719..5176
                     /gene="dtpC"
                     /locus_tag="LGK42_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBK2660148.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5413..6930
                     /gene="lysS"
                     /locus_tag="LGK42_003966"
     CDS             5413..6930
                     /gene="lysS"
                     /locus_tag="LGK42_003966"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK2660149.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7049..>7148)
                     /gene="ghoT"
                     /locus_tag="LGK42_003967"
     CDS             complement(7049..>7148)
                     /gene="ghoT"
                     /locus_tag="LGK42_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBK2660150.1"
                     /translation="SHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1756 a         1677 c         1758 g         1957 t
ORIGIN      
        1 ttttcgtgag tattcttagc gccagtttgg tctggtcagg aaatagttat acatcatgac
       61 ccggactcca aattcaaaaa tgaaattagg agaagagcat gagttctgcc aagaagatcg
      121 ggctatttgc ctgtaccggt gttgttgccg gtaatatgat ggggagcggt attgcattat
      181 tacctgcgaa cctagcaagt atcggtggta ttgctatctg gggttggatt atctctatta
      241 ttggtgcaat gtcgctggcg tatgtatatg cccgactggc aacaaaaaac ccgcaacaag
      301 gtggcccaat tgcttatgcc ggagaaattt cccctgcatt tggttttcag acaggtgttc
      361 tttattacca tgctaactgg attggtaacc tggcgattgg tattaccgcg gtatcttatc
      421 tttccacctt cttcccagta ttaaatgatc ctgttccggc gggtatcgcc tgtattgcta
      481 tcgtctgggt atttaccttt gtaaatatgc tcggcggtac ttgggtaagc cgtttaacca
      541 ctattggtct ggtgctggtt cttattcctg tggtgatgac tgctattgtt ggctggcatt
      601 ggtttgatgc ggcaacttat gcagctaact ggaatactgc ggataccact gatggtcatg
      661 cgatcattaa aagtattctg ctctgcctgt gggccttcgt gggtgttgaa tccgcagcag
      721 taagtactgg tatggttaaa aacccgaaac gtaccgttcc gctggcaacc atgctgggta
      781 ctggtttagc aggtattgtt tacatcgctg cgactcaggt gctttccggt atgtatccat
      841 cttctgtaat ggcagcttcc ggtgctccgt ttgcaatcag tgcttcaacc atcctcggta
      901 actgggctgc gccgctggtt tctgcattca ccgcctttgc gtgcctgact tctctgggct
      961 cctggatgat gttggtaggc caggcaggtg tacgtgccgc taacgacggt aacttcccga
     1021 aagtttatgg tgaagtcgac agcaacggta ttccgaaaaa aggtctgctg ctggctgcag
     1081 tgaaaatgac tgccctgatg atcctcatca ctctgatgaa ctctgccggt ggtaaagcat
     1141 ctgacctgtt cggtgaactg accggtatcg cagtactgct gactatgctg ccgtacttct
     1201 actcttgcgt tgacctgatt cgttttgaag gcgttaacat ccgcaacttt gtcagcctga
     1261 tctgctctgt actgggttgc gtgttctgct tcatcgcgct gatgggcgca agctccttcg
     1321 agctggcagg taccttcatc gtcagcctga ttatcctgat gttctacgct cgcaaaatgc
     1381 acgagcgcca gagccactca atggataacc acaccgcgtc taacgcacat taattaaaag
     1441 tattttccga ggctcctcct ttcattttgt cccatgtgtt gggaggggcc ttttttacct
     1501 ggagatatga ctatgaacgt tattgcaata ttgaatcaca tgggggttta ttttaaagaa
     1561 gaacccatcc gtgaacttca tcgcgcgctt gaacgtctga acttccagat tgtttacccg
     1621 aacgaccgtg acgacttatt aaaactgatc gaaaacaatg cgcgtctgtg cggcgttatt
     1681 tttgactggg ataaatataa tctcgagctg tgcgaagaaa ttagcaaaat gaacgagaac
     1741 ctgccgttgt acgcgttcgc taatacgtat tccactctcg atgtaagcct gaatgacctg
     1801 cgtttacaga ttagcttctt tgaatatgcg ctgggtgctg ctgaagatat tgctaataag
     1861 atcaagcaga ccactgacga atatatcaac actattctgc ctccgctgac taaagcactg
     1921 tttaaatatg ttcgtgaagg taaatatact ttctgtactc ctggtcacat gggcggtact
     1981 gcattccaga aaagcccggt aggtagcctg ttctatgatt tctttggtcc gaataccatg
     2041 aaatctgata tttccatttc agtatctgaa ctgggttctc tgctggatca cagtggtcca
     2101 cacaaagaag cagaacagta tatcgctcgc gtctttaacg cagaccgcag ctacatggtg
     2161 accaacggta cttccactgc gaacaaaatt gttggcatgt actctgctcc ggcaggcagc
     2221 accattctga ttgaccgtaa ctgccacaaa tcgctgaccc acctgatgat gatgagcgat
     2281 gttacgccaa tctatttccg cccgacccgt aacgcttacg gtattcttgg tggtatccca
     2341 cagagtgaat tccagcacgc taccattgct aagcgcgtga aagaaacacc aaacgcaacc
     2401 tggccggtac atgctgtaat taccaactct acctatgatg gtctgctgta caacaccgac
     2461 ttcatcaaga aaacactgga tgtgaaatcc atccactttg actccgcgtg ggtgccttac
     2521 accaacttct caccgattta cgaaggtaaa tgcggtatga gcggtggccg tgtagaaggg
     2581 aaagtgattt acgaaaccca gtccactcac aaactgctgg cggcgttctc tcaggcttcc
     2641 atgatccacg ttaaaggtga cgtaaacgaa gaaaccttta acgaagccta catgatgcac
     2701 accaccactt ctccgcacta cggtatcgtg gcgtccactg aaaccgctgc ggcgatgatg
     2761 aaaggcaatg caggtaaacg tctgatcaac ggttccattg aacgtgcgat caaattccgt
     2821 aaagagatca aacgtctgag aacggaatct gatggctggt tctttgatgt atggcagccg
     2881 gatcatatcg atacgactga atgctggccg ctgcgttctg acagcacctg gcacggcttc
     2941 aaaaacatcg ataacgagca catgtatctt gacccgatca aagtcaccct gctgactccg
     3001 gggatggaaa aagacggcac catgagcgac tttggtattc cggccagcat cgtggcgaaa
     3061 tacctcgacg aacatggcat cgttgttgag aaaaccggtc cgtataacct gctgttcctg
     3121 ttcagcatcg gtatcgataa gaccaaagca ctgagcctgc tgcgtgctct gactgacttc
     3181 aaacgtgcgt tcgacctgaa cctgcgtgtg aaaaacatgc tgccgtctct gtatcgtgaa
     3241 gatcctgaat tctatgaaaa catgcgtatt caggaactgg ctcagaatat ccacaaactg
     3301 attgttcacc acaatctgcc ggatctgatg taccgcgcat ttgaagtgct gccgacgatg
     3361 gtaatgactc cgtatgctgc attccagaaa gagctgcacg gtatgaccga agaagtttac
     3421 ctcgacgaaa tggtaggtcg tattaacgcc aatatgatcc ttccgtatcc gccgggagtt
     3481 cctctggtaa tgccgggtga aatgatcacc gaagaaagcc gtccggttct ggagttcctg
     3541 cagatgctgt gtgaaatcgg cgctcactat ccgggctttg aaaccgatat tcacggtgca
     3601 taccgtcagg ctgatggccg ctataccgtt aaggtattga aagaagaaag caaaaaataa
     3661 ttagctcgta caagggaagt ggcttgccac ttcccttttt tgctcataag gagaacacat
     3721 gaaaacaccc tcacagccgc gcgcgatata ctatatcgtg gcgatccaaa tctgggagta
     3781 cttcagtttt tacggcatgc gtgccttact cattctctat ctcacccatc agcttggttt
     3841 tgatgataac catgccatca gcctgttcag cgcatatgct tctctggttt acgttacccc
     3901 tattctcggc ggctggcttg ccgaccgcct gctcggcaac cgcactgcag tgattgccgg
     3961 cgcgctgtta atgacccttg gccatgtggt gctgggcatt gatacaaatt caacctttag
     4021 cctgtatctg gcgctggcaa tcattatttg tggctacggt ttattcaaat caaacatcag
     4081 ctgtttgctt ggcgagctct acgacgagaa cgatcatcga cgtgatggcg gtttttcgct
     4141 gctgtatgct gcaggcaata tcggttctat cgccgcccca atcgcctgcg gcctggctgc
     4201 tcagtggtat ggatggcatg ttggctttgc ccttgcgggt ggcggcatgt ttatcggttt
     4261 gttgattttc ttaagcgggc atcgtcattt ccagtccacg cgtagtatgg ataaaaaagc
     4321 gctcactagc gtgaaatttg ccttaccagt atggagctgg ttagtggtga tgctctgttt
     4381 agccccagta ttttttaccc tgctgctgga gaacgactgg tcgggatatt tgctggcgat
     4441 cgtttgcctc attgccgcac aaatcattgc ccgcatgatg atcaaattcc ccgaacatcg
     4501 ccgtgctctt tggcaaattg tattgttgat gtttgtcggg acattgttct gggtactggc
     4561 acaacagggc ggcagtacca tcagcttgtt tatcgatcgc tttgtgaatc gtcaggcatt
     4621 caatattgaa gtacctacag cactattcca gtcggtgaat gcaattgcgg tgatgctcgc
     4681 tggggttgta ctggcctggc tggcgtcgcc agaaagccgc ggcaactcaa cattgcgcgt
     4741 ctggctgaag tttgcctttg gcttactgct gatggcttgt ggctttatgt tgttggcatt
     4801 tgatgcccga catgcagcgg ctgacggtca agcgtcaatg ggcgtgatga tatccgggct
     4861 ggcgctaatg ggctttgccg aactctttat tgacccggtg gcgattgcgc aaatcacgcg
     4921 tctgaaaatg tctggcgtat taaccgggat ttatatgctg gcaacaggcg cggtcgccaa
     4981 ctggctggca ggcgtcgtgg cacagcagac gacagagtcg caaattagcg gtatggcaat
     5041 tgcagcttac cagcgattct tttctcagat gggagagtgg acgttggctt gtgtcgcgat
     5101 catcgtcgta ttggcctttg ctacccgttt tctgtttagc acgccgacga atatgataca
     5161 ggagagcaac gattaactgt cgtaaaaaca attggttatg aataaattaa ctttcagttt
     5221 ataacactaa caaatcgaga ttaataagtc atttaaacca ttttgatggt tatttattag
     5281 tgatatcaac ttgaggtaag cgttagtttc gataagataa actgagttac taatagtcga
     5341 ggcagataat acagtgtacc gatctgtctc tttatctacg ctaaattgaa agctggattt
     5401 agaggaacca aaatgtctga acaagaaaca cggggagcca atgaggctat tgattttaac
     5461 gatgaactga gaaatcgccg cgaaaaactg gcggcactac gtcagcaagg tgtggcgttt
     5521 cccaatgatt ttcgccgcga ccatacctct gaccagttgc acgaagagtt tgatgcgaag
     5581 gataaccagg aactggaatc cttaaacatt gaagtctcgg ttgctggccg aatgatgacc
     5641 cgtcgtatca tggggaaagc ctcctttgta acgttgcagg atgtcggtgg ccgtattcaa
     5701 ctgtacgttg caagagatag cctgccagaa ggtgtttata acgatcagtt taaaaaatgg
     5761 gatctgggtg acattatcgg tgcccgcggt acgctgttta agacgcaaac cggtgagctt
     5821 tccattcact gtactgagtt gcgcctgctg acaaaagcac tacgtccttt accagataaa
     5881 ttccatggtc tgcaggatca ggaagtccgt tatcgtcaac gttatctgga cctcatcgct
     5941 aacgataaat cccgtcaaac gtttgttgtc cgttcaaaaa ttctggccgc tatccgccag
     6001 ttcatggtcg cgcgcggctt tatggaagta gaaaccccga tgatgcaggt aattccaggt
     6061 ggggcatctg ctcgcccgtt tattacccat cataacgctc tggatttaga tatgtacctg
     6121 cgtatcgcgc cggagctgta tctgaaacgt ctggttgtag gcggttttga acgggtattc
     6181 gaaatcaacc gtaacttccg taatgaaggt atttctgttc gccataatcc tgagttcaca
     6241 atgatggaac tctacatggc gtatgcggat taccacgatt tgattgaact gacagagtcc
     6301 ctgttccgca ccctggcaca agaggttctg ggtaccacta aagtcactta tggcgagcat
     6361 gtgtttgatt tcggcaaacc gtttgaaaaa ctcaccatgc gcgaagcaat caaaaaatat
     6421 cgtccagaaa ccgatatggc cgacctggat aattttgatg ctgctaaagc attagctgaa
     6481 tctatcggaa ttacggtaga gaaaagctgg gggctgggac gtattgtcac ggagatcttt
     6541 gatgaagtgg cagaagcaca tctgattcag ccaaccttta ttacggaata tccggcagaa
     6601 gtgtcaccgc tggcgcgccg taatgatgtt aacccggaaa tcaccgaccg ttttgaattc
     6661 ttcatcggtg gtcgtgaaat cggtaatggt tttagcgaat taaacgacgc agaagatcag
     6721 gctgaacgtt tccaggaaca ggttaacgct aaagctgcag gtgacgacga agccatgttc
     6781 tatgacgaag attacgtgac tgcgctggaa tatggtctgc cgccaaccgc tggtctgggt
     6841 attggtatcg accgaatgat tatgctgttt acaaacagtc atactattcg cgacgttatt
     6901 ctcttcccgg cgatgcgccc acagaaataa atttcacttt aatgaacgaa gcagtcaggc
     6961 gactgcttcg ttcgtcgttc cgcaaatcca gttttgagga gagacgcttc gtttcacgat
     7021 aaccagcggg tccactcaac tacagctcct aaaagagaga aaaaagtaat gccacaggca
     7081 gactcattgg ccaggttatt ccgaccagga atgcactaag taaacgaata tgtgttttct
     7141 catgtgag
//
LOCUS       DAFAVF010000187         7120 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFAVF010000187 DAFAVF010000000
VERSION     DAFAVF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.189"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..637)
                     /locus_tag="LGK42_003968"
     CDS             complement(<1..637)
                     /locus_tag="LGK42_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK2660151.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENN"
     gene            complement(817..1596)
                     /locus_tag="LGK42_003969"
     CDS             complement(817..1596)
                     /locus_tag="LGK42_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBK2660152.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(1598..2011)
                     /locus_tag="LGK42_003970"
     CDS             complement(1598..2011)
                     /locus_tag="LGK42_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660153.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2543..3469
                     /locus_tag="LGK42_003971"
     CDS             2543..3469
                     /locus_tag="LGK42_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660154.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            3514..3768
                     /locus_tag="LGK42_003972"
     CDS             3514..3768
                     /locus_tag="LGK42_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660155.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            4267..4611
                     /locus_tag="LGK42_003973"
     CDS             4267..4611
                     /locus_tag="LGK42_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBK2660156.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKVFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(4787..5788)
                     /locus_tag="LGK42_003974"
     CDS             complement(4787..5788)
                     /locus_tag="LGK42_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBK2660157.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            6139..6396
                     /locus_tag="LGK42_003975"
     CDS             6139..6396
                     /locus_tag="LGK42_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660158.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            6396..6986
                     /locus_tag="LGK42_003976"
     CDS             6396..6986
                     /locus_tag="LGK42_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660159.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
BASE COUNT         1759 a         1897 c         1876 g         1588 t
ORIGIN      
        1 tgttattttc aatcagatga ttgatttctt cgatcagggc aataacttcc ttacctcctg
       61 ccgaatagcg cccatgaaca gactcagtga ttcccatccc agtctcaatg acatcctcaa
      121 attctgcacg agcagaaatg cggctcttca tcagcttcag atgtttgaac gatggcagga
      181 ttgcatcaag cttagggaat ttcttcttgt taggattaac tttacagaga tacaggtgag
      241 cagttatatc ggtccccatg atagagctga tttcgtccag tactccatca aaatgcatca
      301 aaccaatacg ttcagtcagg gagtcttttg aaggaacaat aacgcagtct gcaaaggcaa
      361 tcgcagtacg ggtaagatct gagtcaaatc ccccacagtc aataagtaac gtcttatcgc
      421 tgttcttgta gggagtcata atttcgatta actcatccac tgttttgggt actttcacgg
      481 aaatttcttt tccttcaggt ctcagcccca gaatgataga aagcccggta tgagtatcca
      541 gatcaactgt atcagacttc atttcaccaa caatgtttac ggatgaagtt gtcttaccca
      601 caccaccttt gggatgcgta ttagcaataa caggcatagt gtatctccag aatccacatg
      661 taatacccat aaagacccca taagacacct ttcggatatc caagaggtac tcaatagatg
      721 tttttttact ctataccggt cctgcatgtc aacagaatat gaaataccga taatatatcc
      781 atgagatatc cgaaagggat ttaaaaaagt cagagcttat ggaattcttt tgagcataga
      841 caccgcatcg gactcaggca tcgaaaactg cacccggtgc cgtgcagcca catccagtgc
      901 aaacaccttc gtgtagacct ccgttgagct gatggactta tgccccatca gactctgcag
      961 gaccttcagc ggtataccgg catacagcat atgcatcgca taggaatggc ggaacgtatg
     1021 tggcgtgacc gggacagaga acgtcactcc atcagcggca gcggcggcaa ccgcctcccc
     1081 aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc cgcgctttct ctgtcctgcc
     1141 tgttcgttta ttacgccgtt ccatggggat tttcagtgtg gccaccatcg tctgcagctg
     1201 gctgacgtac caggagtcgg agagcggaac cagccggtga gtctgctgcc cggcgggcgt
     1261 tcttcctgcc gtcctggcgg ctttttcggt ccgttgtttc agggtcgcaa gctgcacaaa
     1321 cggatacgga ggcgtgagcg aaaaatcccc ccgcgtcagc gccagtgctt cattaatgcg
     1381 cgcaccggtg ttccacagtg tggccagcag catcttgcgg cgcagatccg ggacgtaatg
     1441 gagcagggcg ctcacttcag gggccagcag gtatttgggc agttcatcat gaaccatcga
     1501 catctggcgg agtgccagcg ctgccggata atcgatggca acaggaagct gagcggcatc
     1561 ccgggcatgg tggatcggtg ccggcaggtg ctgcatatca gtgttcactc cacggggaaa
     1621 cgtcgggcag gttttccaca acccggtatt ttcccggata acggagctgg ttaccgccac
     1681 gcgtccagac ggtgacaaca aaaccccctc cctggttgtt ttcggggagc aggcgatcgc
     1741 actgctcact cagcatgtgg atcacgaacg gcgcatcgct gccgtcttca aataccagtt
     1801 ccagcgcatc gcggccatgg agcgttccct tactgatgat gacgtactca gctccccgca
     1861 tctccctgag taaatgtttt gccgcatccg ggacaagcag cctggctgcg ccggcattcc
     1921 agctgaggta gacatatccg gcctgcgcct gtacagactg ccagtaatcc gttctggcga
     1981 cagcctgacc ttcattctca ataaaaaaca tgggtgactc cttttttctt ttccgggcag
     2041 ggacggtcat gcgataacgt catccatcat cactgccctt accgtcacgc cgtctgataa
     2101 catcatgccg ttatcatcat caccatgata acaagcatcc atataaccac gcagttaaat
     2161 taccggagag ccgtttccgt cagatccgac tttccggaat atcacaactt tccggaagtg
     2221 taccacgatg aggaaataat catatcgctg ttttccgccc ctgcacggcg catcacaggc
     2281 aagggaaata acacaacata tagtgtcatc agcagctgaa tgcagcacta catattgtgc
     2341 ttacaggagg tggcgttgtg tggggggaga ggaagatccg tgacctgggg ttttcggatc
     2401 tcagaaaagg aagtgaacag aaaacagaca gcgaagcata cgaattataa aaacgtatgc
     2461 ttcgcagtat ttaaagacac tcaaaaccag gatagagtat aagtattgca gcaatgcccc
     2521 ctcacatgaa aaggtacgcg ggatgagtga aaaagaacat gacatgacaa acgacggtgg
     2581 agagagcgta acgtataccc tgcggaacat ccccgctgac accgaccggg tgatcaccga
     2641 cctggcaagc cacgcccgta agccaaaggc gacgtttctg cgtgagttcc tggaagacag
     2701 cttccgcgac gtcatcgaca gctttgccct gaagaacccg ctcatcgcct ccctggatga
     2761 ggagctggcc agttatctgg acgctaaggt catggagcag cgctaccaga gccattttat
     2821 cacccgctgg aatcaggaat accagaagct gctggggatc tcgacggaag aggagctccg
     2881 ccggttggta ctgaacaaca cccccttcct gcaggtccgt gccgaccagg tgcttaaggg
     2941 gtggaaaaat atcccgcgcg ggatctccct gaccttctcc ctgtttgccg agatcgccgg
     3001 ccgggacagg gaaaccatcg accaggcctg gaaaaatatc ttctactcgc aactgaggga
     3061 aaaaaaacac cgcttttacc aagatatcga ggccatccgc gccctgaaaa aactgcctgc
     3121 cctcaccggc gacagctgga cgcgggatgg catcacggtg cgtatctacc gcccggaaaa
     3181 ttacgcccgc ggcgcatggc gcgttacact gagcctcccg gaaaattacg ccacccagat
     3241 gtggaacatc ccgttcccgg agcttgaata ccgcctcttt accgccgatc cgggctacag
     3301 cgccctgatc agcgccgaac ccgacaggtg ggacaaggcc ttccgttttg tggacggggt
     3361 gtgtgagctt cacctttaca ccaacggtgt ggaagaagat cacaatccca ccccgctcgg
     3421 ggatgtcgct caggcgctga tcaacgtggt ggaagaaaac ctgctgtaac ggacccggat
     3481 ctgcgggcac aactgcatca tcaggaggat acaatgaaag gacgacagag ccgctatgtt
     3541 accggcggag agagtttcgc ggagattgcc cgtctccctt caggggcggt ggtgaggctc
     3601 tgtctgaaca ccggtcttga ggatgcgctg cgggaggcct ccaaatcgct caagtcagcc
     3661 ttcacccgtt ccgggcgaaa atgccggctg tcagcgggta cggcgcaggg gccgtttacc
     3721 ggacgccggc agggcgtggc cacacatctc ttcgtctcgg tactctgagg gggcaggggg
     3781 caaaaaaagt aaaaatgtat tcgccaggtt gcccggaggt gaaggaaaat agacatacag
     3841 cagaacgacg gatagcactt tttgctaaat ggacatcagt attactatgc tatagttgct
     3901 ttaatggata agtgcgcctt gacaaaggcg gtgatttctg ttaacattac tctcatagta
     3961 ttgttccgtc ccgctccacc ccaacaagat ccgtttattt cccgccagac tggttatcac
     4021 cattccggcc cggatttttt tggatttttt tccggggagc ccccggaacg agcttaaaat
     4081 cggtatgaca aacaggagga tgcgaatgaa cacatcataa cagagctgaa agataaaaca
     4141 ttctgtacgg cattaacagc gttcacgtgt gtgaggcgcc gggtgccttt tgacttaaaa
     4201 acgaggttat tgagatgacc aaaatcaaga cagttacttt tgtaaatact tacccgggag
     4261 ggttctatga aaaacttgtt agacaccgag ggaacggttc tattcccatt ccagactgaa
     4321 atccatttta tttggacgat tttctccacc gttaaacgcc tggttatcgg aaccagggac
     4381 catatttgcc agaagcaata ctggagcgcc tgtctctgta ttttgcttct tatggcctat
     4441 gtgggtctct gtgctgcggt ggtctggttt gtagtgccct ggctgaaggt ctttatagtg
     4501 tcgaaatttg cggtttcggc actatgggtc acgccagtaa agcgcggact actctggggt
     4561 atcggtaaag tggttaccgc cacttgccga agatttactc tgctaaagta agtagccgca
     4621 acgctacacg aactgatggt gaatgtcaac agatactcac catctcctta cggcggtggt
     4681 ccctgtgacc actgccgctt tcgcgtgggt gcaacacggc aaaactcctc tgtacaacag
     4741 gggctcccgc cgtcattttc cggcacaggt gaggccggaa ttcggactaa aacgtaaacc
     4801 gcgggccagt ccggtagcgt tcactatcgg ccagcattct ctcaaccaga gagaaatcct
     4861 tttcaccgca gaacacgtac tgctccgcga actccacctg cagggaattg taatgcgtga
     4921 catgctccgg ggtaagcggc ataagaagat agtccgtata cctccggata tgttccagtc
     4981 tttcaaaagg ccgaatgtga cgggggataa gatgcggcgc cctggcgagc agatgttgaa
     5041 gatgctgctt ttgacgaacc atctgttcac ggatggaggg gcaatacatg gccagcatca
     5101 gcgtcgatga cagggggagg taaatctgta tcccgcgaac ggccagacca agattaccgt
     5161 acggaccgaa gtcattgctg ttcttaagca ctacgggatt gtctgatacg taaaacggac
     5221 gttctggcct ggtttccaga aggtaccagt cctttttcag caggagatca atatgtgaca
     5281 cctgctggac gagctgccgc agaaaaatat ccttggtatc gcccccggac gacaggccca
     5341 gggtttccat ggcctgctct ttagtcgagc cgatggcctc catcttatcg gtaagcaccg
     5401 aaatcatgcc tgagatacgc tccagttcac cgaaggttcg tgcgcgctgc acggcaacaa
     5461 acacggccag cctgtagcgt tcatcatctg tcagcctgcg aatatccctg tgcgccagca
     5521 gggctgcaat taccggtgcc gcgtggtgct cgtaaatccc cagcagtggt tcaagactca
     5581 gacggtccgg gtgattatcg aggttgtaga acgtgttgcg gacggtcgcg tcatccggcg
     5641 tggtggcata ggcgcgcccg gcagccttgt caaaggcgta taagcgctgc cgcttaccct
     5701 ttcccggcgt actgaaatgc ttcagtaaaa aacggggaac cgtatgctgc ctgaccacat
     5761 cggtctgcag attgaatgga tcggccataa ctttatctcc ctgaaaagcc acgcaagcgc
     5821 atcggtgcat ctgtattgac ggcatgggca gctggtttga agcttagcat tagaaaacag
     5881 aaccgttcga taatacctgt gtttaacgcc ctgtcagtac cgatggaatt taaatctccg
     5941 gaaccgccgg aaaccggatc tgcgcggcag aaaagacgca aatgatatga taataaaaaa
     6001 tacacataaa atcatatcag atatgatttt catcattgat gataaaacca tatctgatat
     6061 tatcagaaat caggagcaca caaatcattc aggatatgat tattacctgt cagaatatta
     6121 tcagatggag cgtgcaggat ggatcaggaa atgacgtttt cactcagcta tgaacaactg
     6181 acccggtttg cggaacgacg catccgggaa tgcaatctgg acagccaggg cgcgatttat
     6241 ctctgcgagt cagccaaagc cggagccgtg ctgatcttct ggcatgaact ggccataaat
     6301 ggctatgcct caatgaatgc cataaagaga caggagctca ttgacgccga ctttcagcgc
     6361 ctcagaaacc tcatctggcc ggaggatgac aggtaatgac ccagagccgc cgaccgtcgc
     6421 cactgcagcg gagagtgctg attgtgctgg ctgcccttga tgaaaaacgg ccaggacccg
     6481 tgctgacgcg ggacatagaa cgggtgctgg agcgaagcgg ggaagccccg gtctacgggc
     6541 caaacctgcg cgcctcctgc cgtcgtctgg aggatgccgg ctggctgcgt accctgcgcg
     6601 ctccgaacct gcagctggca gtagagctga cggacgccgg ccgggccgtg gcacagcctc
     6661 tgctgctggc agaacaggac cgcctccggg cagaacagcg tgcggcagaa gtcgtcgtac
     6721 tgccgctggt cccggcagca ggtctgcccg cagatggcac ttctgccact gatctggcgg
     6781 tgcaactgaa cggcataacg tatcaggcct gccgtgggga ttttgtggtg cgtctagacg
     6841 gcagcacctg tctgcagtta tggaataaag aaggcagggt ggtacgccgg gagggcgatc
     6901 cgctggaagt ggcgcagtgg ctgcaggcct gtcacgatgc aggaatggag gttcgcgtac
     6961 agataaatga aagtgccgct ccgtaagaac acgcctgtgc acggtttaca catcatccca
     7021 tggtggcagc cggttaactt tcctgcgcca ccagtggaaa gaggccatgc acagtccgga
     7081 caaaaccccc ccccgtgata cttataataa tggcctcacc
//
LOCUS       DAFAVF010000188         7046 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFAVF010000188 DAFAVF010000000
VERSION     DAFAVF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.190"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(25..1161)
                     /locus_tag="LGK42_003977"
     CDS             complement(25..1161)
                     /locus_tag="LGK42_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2660160.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1164..1526)
                     /locus_tag="LGK42_003978"
     CDS             complement(1164..1526)
                     /locus_tag="LGK42_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBK2660161.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2063..3976
                     /gene="mtlA"
                     /locus_tag="LGK42_003979"
     CDS             2063..3976
                     /gene="mtlA"
                     /locus_tag="LGK42_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK2660162.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4206..5354
                     /gene="mtlD"
                     /locus_tag="LGK42_003980"
     CDS             4206..5354
                     /gene="mtlD"
                     /locus_tag="LGK42_003980"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK2660163.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5354..5941
                     /gene="mtlR"
                     /locus_tag="LGK42_003981"
     CDS             5354..5941
                     /gene="mtlR"
                     /locus_tag="LGK42_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBK2660164.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5953..6162)
                     /locus_tag="LGK42_003982"
     CDS             complement(5953..6162)
                     /locus_tag="LGK42_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660165.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6447..6809
                     /locus_tag="LGK42_003983"
     CDS             6447..6809
                     /locus_tag="LGK42_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBK2660166.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1757 a         1779 c         1802 g         1708 t
ORIGIN      
        1 gttccataat ctcccccctc cccctcaatg atccaaataa agataatgca tccagctggt
       61 cattcttagc agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaaccgc
      121 cacctgggcg tagatgccgt cgggtaaggc atcgatatca tcgttaggat ccagttcaat
      181 ggttcccagc acaccgtccg tgccgggcac gaccgttaat gattgcaata ccccctgcgc
      241 ctgataagaa ccgcctggca cgacaggtaa aatactagtc agtttgccgt gaaacacctg
      301 cccaggtagc gcgttaaaca ccacttccgc atcatcgccg ggtttcagac gtaacagcga
      361 gttttgccga aattgggcga caatttgccg tttttgctcg gggatgaaga ccatcaccgg
      421 acgcagcggc aaggcagctg cgtatgtacc tgggcggatc agtacctgag taacgtagcc
      481 attgctcggc gcgcgaatga cagtctgctc aaggttatat tttgcttcag taagttgcgc
      541 tcttaagctc acaatctgcg attgctcgcc gttaaccata ctgtcgagct ggctctggat
      601 ctgcgcctgc tccgccaccg agcctttcac cagcgcatcc tgcgcgagga aattttgccg
      661 cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcaagta
      721 acgttgataa tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg
      781 cgcttcagtg agctgcgcac gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc
      841 ctgaagtctg tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc
      901 cttttgaata agctgattat tcttgtcagt gacttcagta acaattcccg tcacctgtgg
      961 cgtgataggg atcgctatca ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt
     1021 catcaacaaa atcaaaccac tcaccagaaa cacgcctccc agcgccgccg tcgccagcgt
     1081 ccactgattt accggaatgc ggaagatttt aaagaccgcc cacgccagcg ccacgtaagt
     1141 taaaacaatc aatagatcca taattagatc tccgggaacg tggaatagtc agtctttttc
     1201 tcggcggcga gttgatgctc cagccgggaa attcggtcag aaagtgaggc gatttccggg
     1261 tcagttgctt tctcctgcga cgcaacatgt gactgcatac cccagccacg ctccggttga
     1321 tagagcgtcg cccagatcca cagaaacggc cagataacat gcagagtaaa caggctcacc
     1381 cagcccgccg tatgaatagc gtcggcatgg gggtgattgc gctttttggc aatcagataa
     1441 gggatgtcat gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact
     1501 cccaacgcga aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt
     1561 ttgggctggc agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat
     1621 tctcgccata acaccaaaga ataattttta gaggtgatga gttgcttagt tacataacga
     1681 ttgtatgacg aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat
     1741 cttgtgattc agatcacaaa gattcaacaa accatcaaaa caaaaatgtg acactactca
     1801 catttaaatg ccatttttag cgaaaatcgc cgtcttgttg cttttttaca caagcgtttt
     1861 gtgatgagta tcacgtcaat tacctctcta ccccctatat ttatgtgact gatatcacac
     1921 aaaaggccgt cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg
     1981 tccggctacc tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc
     2041 aaacataaga aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg
     2101 tcgtttcctc agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat
     2161 caccgcgtta tttattccaa cagggtggtt accgaacgag acgctggcga agctggtcgg
     2221 gccgatgatc acttatctcc tgccgctgct gatcggttat accggtggta agctggtagg
     2281 cggcgaacgt ggcggcgtag tcggtgccat cactaccatg ggcgttatcg tcggcgcaga
     2341 catgccgatg ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca
     2401 cttcgaccgc tgggtagacg gtaagatcaa atccggtttt gagatgctgg tgaataactt
     2461 ctccgcaggc atcatcggga tgatcctcgc cattctggca tttctcggca ttggcccgat
     2521 tgttgaagcc ctgtccaaaa tgctggctgc gggcgttaac ttcatggttg tccatgacat
     2581 gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat
     2641 taaccacggt atcttctcgc cgctgggtat tcagcagtcc catgaactgg gtaaatccat
     2701 cttcttcctg attgaagcta acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt
     2761 ctttggtcgt ggtagtgcta aacagtctgc gggcggtgcg gcaatcatcc acttcctggg
     2821 tggtatccac gaaatctact tcccgtatgt gctgatgaat ccgcgtctga tcctggccgt
     2881 tatcctcggc ggtatgactg gcgtgttcac gctgactatc ctgggcggtg gtctggtttc
     2941 tccagcatct ccggggtcta tccttgcggt actggcgatg acgccaaaag gtgcttactt
     3001 cgctaacatc gcgggtgtgt gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt
     3061 gctgaaaacc agcaaagtga aagaagaaga tgatattgaa gcagcaactc gtcgtatgca
     3121 ggacatgaaa gctgagtcta aaggcgcatc tccgctgtct gctggcgatg tgactaacga
     3181 cctgagccac gtacgtaaaa tcatcgttgc ctgtgacgcc ggtatgggtt ccagtgcgat
     3241 gggcgcaggc gttctgcgta agaaaattca ggatgcaggt ctgtcgcaga tttctgttac
     3301 taacagcgcg atcaacaacc tgccgccaga tgtggacctc gtcatcactc accgtgacct
     3361 gaccgaacgc gctatgcgcc aggttccgca ggcacagcat atttcgctga ccaacttcct
     3421 cgacagcggc ctgtacacca gcctgaccga acgtctggtt gctgcccaac gccacacggc
     3481 aaacgaagag aaagtaaaag acagcctgaa agacagcttt gacgattcca gtgctaacct
     3541 gttcaagcta ggcgcggaga acatcttcct cggtcgcaaa gcggcaacca aagaagaagc
     3601 gattcgtttt gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca
     3661 ggcgatgctg gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc
     3721 acacggtacg gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta
     3781 cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat
     3841 tgcagcccgt aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga
     3901 tgagtccgtc atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgtt
     3961 ggcaggtcgt aagtaatcca atcccaccct ctccacatgg agaaggtggg gttaattgcc
     4021 tgatgcgcta cgcttatcag gcctacagga tgcatcacga tttgttgaat ttgcacgttc
     4081 ttgtaggccg gataaggcgc ttacgccgca tccggcgctg cccctctcct cacggagagg
     4141 gtttgggtga gggaaaagcc tcaccccagc cctctcgggt aaaaacattg atgaaggtta
     4201 atactatgaa agcattacat tttggcgcag gtaatatcgg tcgtggcttt atcggtaaac
     4261 tgctggcaga cgcgggtatc caactgacgt ttgccgatgt caatcaggtg gtacttgatg
     4321 ccctgaatgc ccgtcatagc tatcaggttc atgtggttgg tgaaaccgag caggtagata
     4381 ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga tgtcgttgat ctgattgctc
     4441 aggtcgattt agtcactacc gccgttggcc cggttgtgct ggaacgtatt gctccggcta
     4501 tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga atccccgttg aacatcatcg
     4561 cctgtgaaaa catggtacgc ggtaccacgc agctgaaagg ccatgtgatg aacgccctgc
     4621 cggaagacgc caaagcgtgg gtagaagaac acgttggctt tgtcgattcc gccgttgacc
     4681 gcatcgtacc gccttcggct tcggcaacta acgatccgct ggaagtgacg gtagaaactt
     4741 tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc actgccgaac atcccaggca
     4801 tggagttaac cgacaacctg atggcatttg tcgaacgtaa actcttcacc ctgaacacgg
     4861 gtcatgctat aaccgcgtac ctcggaaaac tggccggtca tcagaccatt cgtgacgcta
     4921 ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat ggaagaaagt ggtgcggtac
     4981 tgatcaagcg ctacggcttt gacgcagaca aacatgcggc gtacatccag aaaatcctcg
     5041 gtcgttttga gaacccgtat ctgaaagatg atgtagagcg cgtaggccgt cagccgctgc
     5101 gtaaactgag tgctggcgac cgtctgatca agccattgct cggtacgctg gaatacagcc
     5161 ttccgcacaa gaatctgatt caggggattg ctggtgcaat gcacttccgc agtgaagatg
     5221 atccgcaggc tcaggaactg gcagcactga tcgctgacaa aggtccgcag gcggcgctgg
     5281 cacagatttc cggtcttgat gccaacagcg aggttgtatc cgaggcggta accgcttata
     5341 aagcaatgca ataatggtgg accaggcgca ggacaccctg cgcccgaata acagattgtc
     5401 agatatgcag gcaacaatgg aacaaaccca ggcctttgaa aaccgtgtgc ttgagcgtct
     5461 gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc gtcgagcttt tgaccgaggc
     5521 ggtaaatctt ctggtgcttc aggtattccg caaagacgat tacgcggtga agtatgctgt
     5581 agaaccgtta ctcgacggcg acggtccgct gggcgatctt tctgtgcgtt taaaactcat
     5641 ttacgggttg ggcgtcatta accgccagga atacgaagat gcggagctgc tgatggcatt
     5701 gcgtgaagaa ctaaatcacg acggcaacga gtacgccttt accgacgacg agatccttgg
     5761 accctttggt gaactgcact gcgtggcggc gttaccaccg ccgccacagt ttgaaccagc
     5821 agactccagt ttgtatgcaa tgcaaattca gcgctatcaa caggctgtgc gatcaacaat
     5881 ggtcctttca ctgactgagc tgatttccag aatcagctta aaaaaagcct ttcaaaagta
     5941 agcaacgtct gcttactgcc cctctacctg cttcggccga taaagccgac gataatactc
     6001 cagacgttgt agatataacg gcacgctttc atcgggtatt ccggacggaa tcgcgttacg
     6061 gggaggaagt tttttcagat actcccggaa cgcctggctt gatgccatga aatctacggc
     6121 tttatcgata agaagcggaa cgttttcacc taatttgccc atgatgatat ctccgtatta
     6181 ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg ttcccggtaa agatgacatt
     6241 aaggaaaacg tgctgaatcc tcaataatca tccgctgctg tttattatct tttttctata
     6301 gatttcttaa ttaatcaata aattgcgtag tttttaagca tgttaatgat tacgttacag
     6361 aactattgca atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata
     6421 aatttatctt tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaaacgtc
     6481 tcagcgatcg tctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg
     6541 cagacaaata tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc
     6601 tgcgtgaagt tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt
     6661 ttcagcgcgc aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc
     6721 gcggactggt ggttgtgcac ccaatgaccg cgctgggccg tgaaatgggc ctgcaggaga
     6781 tgactggatt ctctaagact gcgttttaag cgcctcagac gactggttcg atcatatcaa
     6841 cccgcatctg gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt
     6901 accaaacaat tcactccctt tacttcgatt taccgctggt tgatattctg aaaatgcatt
     6961 tttaccgtaa tagccatcta taaacaataa aaatatatag aagatatcat ttgttaatta
     7021 acaagcattt acgtctcttt ttttcg
//
LOCUS       DAFAVF010000189         6985 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFAVF010000189 DAFAVF010000000
VERSION     DAFAVF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.191"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1061
                     /locus_tag="LGK42_003984"
     CDS             <1..1061
                     /locus_tag="LGK42_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK2660167.1"
                     /translation="IQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKR
                     LISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQLLESELF
                     GYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGAS
                     SPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYF
                     LHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLP
                     PNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQVADELG
                     IGIATLYRKIKKYELLNI"
     gene            complement(1101..1580)
                     /gene="xdhC"
                     /locus_tag="LGK42_003985"
     CDS             complement(1101..1580)
                     /gene="xdhC"
                     /locus_tag="LGK42_003985"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBK2660168.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(1577..2455)
                     /gene="xdhB"
                     /locus_tag="LGK42_003986"
     CDS             complement(1577..2455)
                     /gene="xdhB"
                     /locus_tag="LGK42_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBK2660169.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(2466..4763)
                     /gene="xdhA"
                     /locus_tag="LGK42_003987"
     CDS             complement(2466..4763)
                     /gene="xdhA"
                     /locus_tag="LGK42_003987"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBK2660170.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5178..5933
                     /locus_tag="LGK42_003988"
     CDS             5178..5933
                     /locus_tag="LGK42_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK2660171.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6012..6085
                     /locus_tag="LGK42_003989"
     tRNA            6012..6085
                     /locus_tag="LGK42_003989"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6044..6046,aa:Gly,seq:ccc)
     gene            6307..>6985
                     /locus_tag="LGK42_003990"
     CDS             6307..>6985
                     /locus_tag="LGK42_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660172.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDL"
BASE COUNT         1772 a         1690 c         1776 g         1747 t
ORIGIN      
        1 acattcaggg ccgacaatta tttttaatgg catttcacca atcgcatacc agtttttctg
       61 tagcaaatgc acctgatgaa ccacatattg aacaattggt tggcgagtgc cgtgttatgc
      121 ggcaattaaa acgactcatt agccgtattg cacccagccc atccagcgtt atggtggttg
      181 gtgaaagcgg cacgggtaaa gaagtcgtcg cccgagcaat ccataagttg agcggaagac
      241 ggaataaacc ctttattgct atcaactgtg ccgcgattcc ggagcagctt ctggaaagcg
      301 aactgttcgg ttatgttaaa ggcgcattta ctggcgcttc tgccaacggt aaaacagggt
      361 tgattcaggc ggcgaatacg ggcacgctgt ttctcgatga aataggtgat atgccattaa
      421 tgttgcaggc taaattactg cgcgctattg aggcgcgtga aattctgccg attggtgcca
      481 gtagcccaat acaagtcgac attcgcatca tttctgcaac taatcagaat ttggcccagt
      541 tcattgccga aggtaaattc cgcgaagatc tcttctaccg acttaatgtt atcccgataa
      601 ctctgccacc gctgcgtgaa cgtcaggaag atattgaact attggtgcat tactttttac
      661 atctgcatac ccgtcgtctg ggatcggttt atcctggcat tgctcccgat gtcgtcgaaa
      721 tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt aagcaatttg atggaatatc
      781 tggttaacgt agttccttca ggtgaagtta tcgacagcac gctattgccg ccaaatctgc
      841 tgaataatgg cacaacggag caaagtgatg taacagaggt cagtgaggcg cacctgtcac
      901 tcgatgatgc gggcggcacg gcgctggagg agatggaaaa gcaaatgatc cgcgaggcgc
      961 tttcacgtca taacagcaag aagcaagttg ctgatgaact gggcatcggc attgctacgc
     1021 tctatcgcaa gattaagaaa tatgagttgt taaacatata acctcagtga atccggcctg
     1081 aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat ccagaactgt atttacaatc
     1141 atctgatacc ccgtgcagcg acaaagattt cccgccagtc cgcgacgaat ttccgtaatg
     1201 gttaatggct tctcgcgtgg tttcgccagc attgccgtgg tagccataat caggccaggc
     1261 gtacaaaacc cgcactgcac tgcgccggat ttcgcataag cctgctgaac atgagaaagt
     1321 tttccgcctt tcgcttcacc ttccagcgtg cggatctctt ttccttcagc ccaggcggca
     1381 aggtataagc aactgtctat tgctgtgccg tcgaccaaca ccgtacaggc accacattca
     1441 cccacgcagc acccttgttt gacacttagc agtccttgtt cgcggagtaa ttccgagagc
     1501 ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt taatggtgca ttcgatggta
     1561 attgtttcgc tgtgattcat tgcaattttc cccccgccgc ggcgacggct tcgctaatca
     1621 cttttttggt catcgtctgg atgagatgca gacgaaactc tttactggcc cgccatgaag
     1681 aacgcggggc gacatcttgc aggacagatt cgctgatagc ttccagcgtt tgcaggttta
     1741 atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg aatcggcgtt ggcgcggcaa
     1801 caccaaatgc caggcgtaat tcgctgaaat tgccgttatc cagtcggcaa tgtgcggcgc
     1861 agccaatcgt tgaaatatcc attgcgtcgc gcatggcata tttaaaatgc gcgctgcccg
     1921 cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag gatttcgtca tgctcaagag
     1981 acactttgcc cggcccggtg tgaaagccat taatcgggac gaaacgaaca ccgcgcgggg
     2041 agtggatctc cagtttcgcg tcataaatta gcgttggcgt ggcagaatct gcgctggtgg
     2101 caccgttgca aatatttcca ccgtaggtag cgacgttacg gatctgcggt ccagcaatgg
     2161 acgtggccgc agcacataac gccgggagat gacgttgagt tataggatct tctattagct
     2221 gggtaaatgt cgttgcagag ccgatacgta gcgagccatc ttccgccagc gtaattcccc
     2281 gcagctccgc cagattatga atatcaacaa tatggcgata acggtcattg tgatggtgga
     2341 gctgaatcag tacgtcagtg ccaccggcga gcagtttggc ctgcgggttg tcagccagca
     2401 ggttgatggc atcggcaagg gttgctgcgc gatggtaaga agcaaaatca aacatgatgt
     2461 tatcctcaaa tcaatcctgc cagatggaac tcttcatata accgttttgg cgtcagcggc
     2521 agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt tacgaatagc agcggcaaca
     2581 ggaattattg gtggctcacc cagtgactta tgtccgtatg cggattgcgg ctcattgatt
     2641 tcgacgaacg cgctttccag ttgtggcaga tccggcatgg tcggcatttt gtaatccagc
     2701 agattggggt tacggaccac gccgctttta gcatcgatga tcatctcttc aaatagcgcc
     2761 cagccaatgc ccattcccat tccgccgtgt acctgacctt ctgccagcag tggattaaga
     2821 atatgccctg aatcatgaac gttgaggatg cggttgatga tgactttgca cagcgcaata
     2881 tcgaccgtca gatcaacaaa ggtacagcca aacgccggtg ggttagtggt ggttttgatg
     2941 gagctttcag cagagagctg cccgccgcgt tcagggtggt agaaagcgtc catcgccaaa
     3001 tcttttaacg acattaacgg ctcttccggt cgttcaacca gcacgatatg gccttttatc
     3061 agggtcagat tcatcgctga ctgatgtagc atgactgcgg cgtgagcgat gattttctct
     3121 tttaataata gtgccgcact gcgcagcgca ggcgcggcaa catagctctg gcgtgaggca
     3181 aatgcgccgg gatcgaacgg cgtaacgtcg gtatcttgag ttgaaataac gcgaacgtcg
     3241 ctgaccggaa cccccacggt ttctgccacc atttgcgaga agacggtgtc ggcaccctga
     3301 ccgatttccg tcgcgccgct ttgcacgttg atggttccat cctgattcat cagaaggcgc
     3361 gcgcctgcta tttctacgcc gacaggccag gtgttagagg tgtagctaaa acaggcgacg
     3421 ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt ctgcacggcg tttttcccat
     3481 tcaaagattt tccggccttt ttcaagacac tccggcaacc ctgcgctgta aatacgtttg
     3541 cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc gtaaacgaat ttcaacagga
     3601 tcaataccta acgctgtcgc ggcgtcatca agcatagact caacggcaaa tacgacttgt
     3661 ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt tggtatagca ggtctttgaa
     3721 ctgtaagcgt aggcacaacg aggataaagg taagcgactt tattcccccc cgcagaagcg
     3781 atggagtgcc cgtgagatgc ataagcgccg gtgttagaca gaacatccag actataacct
     3841 ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa tggtaaaagc gtggcgggta
     3901 cgggttgcga ggaaacactc ttcacggcta agggaaactt tcaccggaat gccgccaagc
     3961 ttgctggtca ggaatgccgc cattggctct tccagtacat cctgtttatt accaaaaccg
     4021 ccaccgacaa acggtttgat gactcgtacg catgaccagg gaatatccag cgcctgacca
     4081 accacgcggc gaacaatgtg cgggatctgg gtgctggaaa cgatggtaat tcgcgagtca
     4141 tcctccatcc acgccagcga tgttacgctt tccatgtgac aatgttgaat aacgggggtc
     4201 tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg tttgttggac attacccgtc
     4261 gacatcgtgc tttgtttcag taaattgcca ccgttatgga ttggtgcagc gtcttctgcc
     4321 agcgccgctt ctggcgtggt gataacgggt aattcttgcc actcaatgct gaccaattgc
     4381 gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga tggcaacggc gtcaccatga
     4441 tgacgaacat ggcgagttag cagtgcgcga tcggcggtat cgcgcttgtt ttcgtcaagt
     4501 gtccaggcat gcccagcagt agcaaatgga atatcaggca catcttccca ggtaaaaatc
     4561 gccagtacgc ccggcaaact tctggcttgt tcatcattaa tacttacggc ataaccatgt
     4621 gcgatagggc tacgtacata tttcgcataa cacatgcccg ccataacata atcgtcagta
     4681 tatcgtgccc gcccggtgac cttagcaatg gcatcgacgc gcatgcatga ttcacccgta
     4741 gcggttgctt cccgcgcttc catagaaatc ccctcgattt gaacatttgg ttcttatggc
     4801 aaagatttag tctaagcagg caagatttac gccagaaacg acgataagcg ttaggggagg
     4861 taggaagtgt gatgacgtta aaaaaacaca gatatgatac gaaacaggac tctgaatagg
     4921 ggcagtgata cgtatcaata tgagtggcga taaaatcaat gtgataaatt gggattatta
     4981 cgcatcagga tgggaaatag ggtaaaaagt caggaagttc gatacctcta cagcgaatat
     5041 tttgctgatg atacttccct ttgccaaatg aaattaacct ctttctctat taccacgttt
     5101 ttccagaagc aagagattgg gtatcaagag gctggctgct atgataaggc gtcttgtttt
     5161 ttaagcgagg aaagattttg agtgcgggac gcctgaataa aaaatctctg ggtatcgtga
     5221 tgttgttatc ggttggactg cttttggcgg gctgttcggg tagcaaatca tccgatacag
     5281 gaacgtattc cggctccgtt tacaccgtga aacgggggga tacgctatat cgtatttcgc
     5341 gcaccacggg aaccagcgta aaagaactgg cgcgactgaa cggcatttcc cccccttaca
     5401 ccattgaagt tggtcagaaa ctaaaactag gtggggcgaa aagtagcagt agtacacgta
     5461 aatcaaccgc caaatcaacg accaaaaccg catcggttac accgtcatca gcggtaccga
     5521 aatcttcctg gccgccagta gggcaacgtt gttggttatg gccaacgaca gggaaagtta
     5581 tcatgccgta ttcgacagca gatggcggca ataaagggat tgatatctca gctccacggg
     5641 gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac cagctgcgtg
     5701 gctacggtaa tctcatcatg attaaacaca gtgaagatta cattacggct tacgcccata
     5761 atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa atcgccacta
     5821 tggggagcac ggatgcggca tctgttcgcc tgcatttcca gattcgttac cgtgcaacgg
     5881 caattgatcc gctacgttac ttgccgccgc agggcagcaa gccaaaatgc tgatggcgaa
     5941 ttaatcagca gtcagcagcg cggcacttgc taaggagagc gtaaggttta taatgcctta
     6001 cgcatctcga agcgggcgta gttcaatggt agaacgagag cttcccaagc tctatacgag
     6061 ggttcgattc ccttcgcccg ctccaattta tcttcacctg atcaatctgc catttttgct
     6121 gtatctgatt ttctatatta tcttaattca atcgcttcag acttttcgca atcgccgtaa
     6181 tacttcccca ttttgttgta aataaaaagc gtttacgcaa tgcaatcctt ttgtaacaac
     6241 ccaggtaaaa caccttattt tcaagttgtt aaataagtgg ttatctacgc ttttaacaaa
     6301 cgattgatgg aatattttat gaaagggaaa tctgcattaa ctcttctgct cgctggtata
     6361 ttttcgtgcg gcacatgcca ggcgacaggg gcagaagtga cgagtgaatc tgtatttaac
     6421 attcttaatt ccacgggggc agcaacagat aaaagttatt tatcgttaaa tccagataag
     6481 tatcctaatt atcgtctttt gattcactca gccaaattac aaaatgaaat aaagagccac
     6541 tataccaaag acgaaattca aggattgcta acattaacag aaaacacacg aaagttaacg
     6601 ttgacggaga aaccctgggg aacgtttatt cttgcttcta ctttcgaaga tgacaaaaca
     6661 gcggcagaaa cccactatga tgctgtctgg ctgagggata gtttatgggg ctatatggca
     6721 ttagtatcag atcagggaaa tagtgtagcc gcaaaaaagg ttctgctcac cctgtgggat
     6781 tatatgtcta cgcccgacca gattaagcgg atgcaggacg tcattagcaa ccccaaacga
     6841 ctggatggag ttcccgggca aatgaatgtt gtgcatatcc gatttgacag caactccccc
     6901 gtgatggcag atgtgcagga agaaggcaag ccgcagctgt ggaaccataa acaaaatgat
     6961 gcgttaggtc tttatcttga tctcc
//
LOCUS       DAFAVF010000190         6953 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFAVF010000190 DAFAVF010000000
VERSION     DAFAVF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.192"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            72..1505
                     /gene="cscA"
                     /locus_tag="LGK42_003991"
     CDS             72..1505
                     /gene="cscA"
                     /locus_tag="LGK42_003991"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK2660173.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1513..2571)
                     /locus_tag="LGK42_003992"
     CDS             complement(1513..2571)
                     /locus_tag="LGK42_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK2660174.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2739..2957
                     /locus_tag="LGK42_003993"
                     /pseudo
     CDS             2739..2957
                     /locus_tag="LGK42_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2975..4303
                     /gene="dsdA"
                     /locus_tag="LGK42_003994"
     CDS             2975..4303
                     /gene="dsdA"
                     /locus_tag="LGK42_003994"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBK2660175.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4411..5949)
                     /gene="emrY"
                     /locus_tag="LGK42_003995"
     CDS             complement(4411..5949)
                     /gene="emrY"
                     /locus_tag="LGK42_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBK2660176.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(5949..>6953)
                     /gene="emrK"
                     /locus_tag="LGK42_003996"
     CDS             complement(5949..>6953)
                     /gene="emrK"
                     /locus_tag="LGK42_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBK2660177.1"
                     /translation="ADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKA
                     KNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEH
                     TKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKS
                     PVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDL
                     YGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLR
                     IGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF
                     "
BASE COUNT         1895 a         1661 c         1637 g         1760 t
ORIGIN      
        1 agtaagggac tttattttta taaaagttaa cgttaacaat tcaccaaaat tgcttaacca
       61 ggatgattaa aatgacgcaa tctcgattgc atgcggcgca aaacgcccta gcaaaacttc
      121 atgagcaccg gggtaacact ttctatcccc attttcacct cgcgcctcct gccgggtgga
      181 tgaacgatcc aaacggcctg atctggttta acgatcgtta tcacgcgttt tatcaacatc
      241 atccgatgag cgaacactgg gggccaatgc actggggaca tgccaccagc gacgatatga
      301 tccactggca gcatgagcct attgcgttag cgccaggaga cgataatgac aaagacgggt
      361 gtttttcagg tagtgctgtc gatgacaatg gtgtcctctc acttatctac accggacacg
      421 tctggctcga tggtgcaggt aatgacgatg caattcgcga agtacaatgt ctggctacca
      481 gtcgggatgg tattcatttc gagaaacagg gtgtgatcct cactccacca gaaggaatca
      541 tgcacttccg cgatcctaaa gtgtggcgtg aagccgacac atggtggatg gtagtcgggg
      601 cgaaagatcc aggcaacacg gggcagatcc tgctttatcg cggcagttcg ttgcgtgaat
      661 ggaccttcga tcgcgtactg gcccacgctg atgcgggtga aagctatatg tgggaatgtc
      721 cggacttttt cagccttggc gatcagcatt atctgatgtt ttccccgcag ggaatgaatg
      781 ccgagggata cagttaccga aatcgctttc aaagtggcgt aatacccgga atgtggtcgc
      841 caggacgact ttttgcacaa tccgggcatt ttactgaact tgataacggg catgactttt
      901 atgcaccaca aagcttttta gcgaaggatg gtcggcgtat tgttatcggc tggatggata
      961 tgtgggaatc gccaatgccc tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc
     1021 gcgagctatc agagagcaat ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt
     1081 tacgccagca gcatcaatct gtctctcccc gcacaatcag caataaatat gttttgcagg
     1141 aaaacgcgca agcagttgag attcagttgc agtgggcgct gaagaacagt gatgccgaac
     1201 attacggatt acagctcggc actggaatgc ggctgtatat tgataaccaa tctgagcgac
     1261 ttgttttgtg gcggtattac ccacacgaga atttagacgg ctaccgtagt attcccctcc
     1321 cgcagcgtga cacgctcgcc ctaaggatat ttatcgatac atcatccgtg gaagtattta
     1381 ttaacgacgg ggaagcggtg atgagtagtc gaatctatcc gcagccagaa gaacgggaac
     1441 tgtcgcttta tgcctcccac ggagtggctg tgctgcaaca tggagcactc tggctactgg
     1501 gttaacataa tatcaggtgg aacaacggat caacagcggg caagggatcc gcgtcactct
     1561 tcccccttca cgaccttcaa taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg
     1621 aagctgaatt gtggtcagcg gcggtaaaaa cagatgcccg acgccaacca gattatcaaa
     1681 gcccattacg gcgacatcct gcgggattcg tacccccttc gccagaagaa cctgataagc
     1741 cacaaaggct gcgcgatcgt taccacatat cagaacatca aaatctggtt tgcccgattt
     1801 gaagtggtca ttgagtaaac ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg
     1861 aaattgtttc acctcagcca gatctcgtcc agcatcacgc caggcctgct caaatccctg
     1921 ccgacgatac cctgttgcca acgcactttc cggtagccag aagcataacg gttgacgata
     1981 gcccgccgcg agcaaatgct gtgttgattc atattgtgca gtgtaatcat cagggatata
     2041 actgggtaac gctgggtcat ccgccacaca gttcgccaat acaatatttt caccatacag
     2101 agactcaggc agcgtgatat gtcgcagccc cattgtagta tagataatgc catccggacg
     2161 gtgggcaagc agctgacgtg ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa
     2221 aaaactattc cagccgaact cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga
     2281 gaaaggagtg gtagccgtgt cctgcgccag cacggcgaga gtcgacggct tacgtccttg
     2341 agcgcgcatc ttacgggcgg aaagatcagg aacataattc agggtctgga ttgcctgcaa
     2401 tacgcggtca cgcgttgcag gacgcacaga ttctgcatta tgcatcaccc gggagactgt
     2461 catcatcgac actcccgcca ggcgtgcgac atcctttaat gaagccatac ccaagccgtt
     2521 tgccgtaaaa cgggcactgt agcagaaaca gacgtcactg gcgagatcca acgccctatc
     2581 acctgacaca gcaatacaat aaaaaataac aataattccc ggacaattgt ccccaattcc
     2641 gcctctgttc tcgccaacga gtaaccatat aggcaagtaa gtaaaacatg ccaactggca
     2701 tcgccaacct ggagaagaaa atataaatat ggatcattgt gctgccgctg tatcccgaca
     2761 tcagcccgga aattattgcg attgctatcg gttcaggtgc aattggctgc acgatcgtta
     2821 cggactcgct tttctggcta gtgaagcaat attgcggcgc tacgctcaat gaaacattta
     2881 aatactatac gacagcgaca tttatcgctt cagtcgtcgc tctggcgggc acattcctgc
     2941 tgtcatttat catctaagcg caaagagacg tactatggaa aacgctaaaa tgaactcgct
     3001 catcgcccag tatccgttgg taaaggatct ggttgctctt aaagaaacca cctggtttaa
     3061 tcctggcacg acctcattgg ctgaaggttt accttatgtt ggcctgaccg aacaggatgt
     3121 tcaggacgcc catgcgcgct tatcccgttt tgcaccctat ctggcaaaag catttcctga
     3181 aactgctgcc actgggggga ttattgaatc agaactggtt gccattccag ctatgcaaaa
     3241 acggctggaa aaagaatatc agcaaccgat cagcgggcaa ctgttactga aaaaagatag
     3301 ccatttgccc atttccggct ccataaaagc acgcggcggg atttatgaag tcctggcaca
     3361 cgcagaaaaa ctggctctgg aagcggggtt gctgacgctt gatgatgact acagcaaact
     3421 gctttctccg gagtttaaac agttctttag ccaatacagc attgctgtgg gctcaaccgg
     3481 aaatctgggg ttatcaatcg gcattatgag cgcccgcatt ggctttaagg tgacagttca
     3541 tatgtctgct gatgcccggg catggaaaaa agcgaaactg cgcagccatg gcgttacggt
     3601 cgtggaatat gagcaagatt atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc
     3661 tgacccgaac tgtttcttta ttgatgacga aaattcccgc acgttgttcc ttgggtattc
     3721 cgtcgctggc cagcgtctta aagcgcaatt tgcccagcaa ggccgtatcg tcgatgctga
     3781 taaccctctg tttgtctatc tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt
     3841 cgggcttaaa ctggcgtttg gcgatcatgt tcactgcttt tttgccgaac caacgcactc
     3901 cccttgtatg ttgttaggcg tccatacagg attacacgat cagatttctg ttcaggatat
     3961 tggtatcgac aaccttaccg cagcggatgg ccttgcagtt ggtcgcgcat caggctttgt
     4021 cgggcgggca atggagcgtc tgctggatgg cttctatacc cttagcgatc aaaccatgta
     4081 tgacatgctt ggctggctgg cgcaggaaga aggtattcgt cttgaacctt cagcactggc
     4141 gggtatggcc ggacctcagc gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt
     4201 cagcgcagaa caattgcgta atgccactca tctggtgtgg gcgacgggag gtggaatggt
     4261 gccggaagaa gagatgaatc aatatctggc aaaaggccgt taataacgtt tcaacgcagc
     4321 atcgcaatcc tttccctggg tgagcgatgc tgccgatggc gcagacttaa gatccccggt
     4381 cttacccgct ataaccccct ccttttatca tcacccaatg cctttcgctg taaacggcgg
     4441 tttcgcaaac caaaccaaaa ccgtaagtaa gataaaagca atagccgcca tacggaaaat
     4501 ctcatttgca gaaatagaaa gtgactgctg ggttatttca ttattaattt cattaagaac
     4561 tcctgaaagc gaaccataat atttatccat aatttgtgac gatgaattaa acacggggtt
     4621 aaattgatcg atggttgctg tcaactgact atggtgtaac gattcgcgtc gtccccacag
     4681 cgtcattgtc aacgacgtac caactgatcc tgacaaggta cgaaaaaaat tactcatact
     4741 cgaggcattg gcaaatttat tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa
     4801 aaagaaacag gcaacggcga atccctggaa aaactgtggc aaaatgatgc ctgtaaaatc
     4861 aatcgttggc ataaatgtca cagaacgcca gtaatagcaa accgcataca tcaaaaaact
     4921 aaatgtcact aacaaccgca tgtctatttt gttgccataa cgtcctatca aaggtgaaat
     4981 taatagtggc atgatgccga tgggcgcata agcaagtccg gcccatatcg cattataccc
     5041 catcgtttcc tggagtaact gcggcataag gacgatcgct ccagagtaaa ataaatacgc
     5101 gcatgtgata ctcacaatac caatggtgaa gttacgggac ttaaacaaac tgagatcaag
     5161 aatcgggttc tctgaggtcg actcccaaat gactaaagag atcagagaaa taactgatac
     5221 tactgttaat attattattg tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag
     5281 cataatttgc aagccaccaa caccgagcac taacagggtc agtcctggta gattcatttt
     5341 gaccggtgaa gtttcagttt ctcttccttt aagtaaggtt aagcataatg tcaggacgat
     5401 aatccccata gggacattga ttaaaaatat ccaaccccag ctaaagttat cgcaaatata
     5461 accgcccaat atcggcccac atatcggagc gataatcacg gtcattgacc ataatgccag
     5521 agcaaatgtt cttttttctg gtggataatt ccttaataat aaactctgtg acagtggaat
     5581 taacggcccc gccattaatc cctgaacgac tctaaaaaat atcagcacat caagattggt
     5641 cgataaacta cacattaagg aagacagcga aaaaaaagtg actgaaagta aaaataacct
     5701 taattcgcct attctttgtg ccagcctgcc ggtaacaggg atcgcaatgg catttgctac
     5761 gccaaacgag gtgataaccc aggtgccttc gtctgttgat gctcccagaa agccagatat
     5821 tgtcggtatt gcgacgttag aaatagtgga atccaacatt tgcataaacg tcgctaatga
     5881 caatgcaata gtaacgcacc ataacgtccc accggttaat ggtgccggag tcgatttagt
     5941 gattgccatt aaaattgtcc attatgcgaa ataatgttgc taatttcttt ttctatcgga
     6001 ctggtatcga taactaaagc cttactggta taagccggca tggaggtcac ggttgaagcc
     6061 agctcaggca tctcggcaat gtcttcgttc ttcgtatcaa tagttgctgt catcgataaa
     6121 ccaatacgca agggatgttc catgagttct tttggatcaa gagaaacttc aaccggtaca
     6181 cgctgaacga ttttgatcca gttccctgtc gcattttgtg caggtaataa ggagaacgca
     6241 ttgccggttc ccatattgat ccctgtcacc cgaccatgaa acacaacatt ttcaccataa
     6301 agatcgctga taatattgac cgattgacca atccgtacat ccgtgagttg tgtttcttta
     6361 aagttggcat taacccacat ttgacgtgcc ggtacgacag ccattaacga ttgtccgggg
     6421 ctcactgttt cgccgacctg aacacttcgc tgggcaatat agccggtaac cggactctta
     6481 atatccgtac gtttaagcgc caaccaggct tctttagttg catccgccgc ttcaacgact
     6541 tgtggctgac ggtttaatgg tgtgttcatt actaaagctt tattcgcttt ataagcctgg
     6601 atagcggcat tcaatgccgc tttgctactt attaacgtat ctttggtatg ctccagcgtt
     6661 tcttttgaaa taaccccctg cttcgctaac ggcactcgac ggttataatc ttctaaagat
     6721 tgttgatact gaatacgtgc tgaagcgact tcggcactgt attgtttatc ctgtaagtat
     6781 agtttattcg tttgccgaac aatatttgcc agattatttt tagctttatt gagtgcgata
     6841 gtggcatcag ttttatccag tgaaactaaa atgtcacctt gtcgaacgta gttcgtatct
     6901 ttatgattaa cgacagtgac actacctgag acttgtgcag aaattggatc tgc
//
LOCUS       DAFAVF010000191         6905 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFAVF010000191 DAFAVF010000000
VERSION     DAFAVF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.193"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            36..1289
                     /locus_tag="LGK42_003997"
     CDS             36..1289
                     /locus_tag="LGK42_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK2660178.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFITIILLCAYFKAQSDQCLLEK"
     gene            complement(1359..2330)
                     /gene="rfaC"
                     /locus_tag="LGK42_003998"
     CDS             complement(1359..2330)
                     /gene="rfaC"
                     /locus_tag="LGK42_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBK2660179.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQVENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(2334..3380)
                     /gene="rfaF"
                     /locus_tag="LGK42_003999"
     CDS             complement(2334..3380)
                     /gene="rfaF"
                     /locus_tag="LGK42_003999"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBK2660180.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEV"
     gene            complement(3390..4322)
                     /gene="rfaD"
                     /locus_tag="LGK42_004000"
     CDS             complement(3390..4322)
                     /gene="rfaD"
                     /locus_tag="LGK42_004000"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBK2660181.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4536..5732
                     /gene="kbl"
                     /locus_tag="LGK42_004001"
     CDS             4536..5732
                     /gene="kbl"
                     /locus_tag="LGK42_004001"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK2660182.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5742..6767
                     /gene="tdh"
                     /locus_tag="LGK42_004002"
     CDS             5742..6767
                     /gene="tdh"
                     /locus_tag="LGK42_004002"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK2660183.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT         1697 a         1657 c         1683 g         1868 t
ORIGIN      
        1 aaataaccca tcctcgtagc ataggttgaa actatatgtc gttttgttgg aatgaaatta
       61 attctggtat aaagtcttta attctcatat tatgtatttt ttctttaatg actttgtctt
      121 tatgggatga tgttgcaaca aagtttcttc atgcagctgg aattatatct gcattgtatt
      181 ttcttgcgac accaaaaaaa acaataacta ataatcctac tttgttaatt ttcatctcat
      241 tatgtctttt gggtatcgta aatatcatct ggtattcaca ttataaagtt tcaggctctg
      301 tttataccaa tgcatatcgt ggcccaatgg aaactggaaa aattgccttg tgtagcgctt
      361 ttattttctt agttcttttt gctaaaaatg agatgagaac aaaaataaaa tttgggaaac
      421 taattctgtt cgcatccctg gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac
      481 atttctattt aaacgtcgac cgtgttgcat tatcagcttc ccacgctaca acagcaggct
      541 acatcatcct ttttccttct ttactggcat caattctcat tttaaaatcc gacttaagac
      601 ataaaacaac attatataca attaacttca tgcttagttt atgtgctgtc atagtaactg
      661 agacgcgtgc agccatatta gtgtttccat tctttgcgtt aatattaatc gtaatggata
      721 gttatattaa taagcgaatt aattataagt tatattgttt tattacgatt gcattattag
      781 caggtgtatt ttcttttaaa gatacattgc ttatgagaat gaatgactta aataacgatt
      841 tagttaatta ttcgcatgat aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg
      901 aagttggctt aaaaacatat tctccaatag gacaatcact ggaaaaacgt gcggaaaaaa
      961 tacatgaact agaagaaaaa gagcctagat tgagtggcgc tttaccctat gtagattctc
     1021 atttgcataa cgatctcata gatacgttat caacgcgtgg tattcctgga gttgtattaa
     1081 caattttagc attttcagca atactcatat atgccttaag aactgctaaa gaaccttata
     1141 ttttaatctt gcttttttca ctactggtag taggactaag tgatgtaata ctcttttcta
     1201 aaccggttcc gactgctgtg tttatcacca taatattgct ttgtgcttat tttaaagcac
     1261 aatcggacca atgtttatta gagaagtaat gaatatgtga aataaaatca gcaaggaagc
     1321 tgattttatt tcaaaattat ctattaaaga gttgtaattt aaatcatggc agcgttttct
     1381 tcaatgaacc gcttaacagc atttgctgtc aattgagaca actcattccc cggagcccta
     1441 caaaccatct gattcttccc atacccacca attaatcccg gatcggtagg tccataaacc
     1501 gtgatattgg gtctatccag tgccgctgtt aaatggctta accccgtatc cactgacact
     1561 acaaatttag ccccggccag cacgcgggca acgccttcca gactcatctt cggcaatact
     1621 tcaacataag caaatccttc cgccagtcgt ttcgcccgtt cttcctcatg cggcgcgccc
     1681 cacggaagtt taatccgtat tcctgaatca gccagtaaac caatcaattc tcgccagtgt
     1741 tcttccggcc agtgtttatc atcacgggtc gtcgcatgaa gaaatacggc atattcgcca
     1801 gcatctgtag gcaggttcgt caggaaatgc tgtgcaatag cataatcgcc ctgggtttgc
     1861 ggtttgctat agcccagact tttggcaaac agttcgcggg tgcgttctac ggcgtgctgc
     1921 tgttttgcaa tatgatgctt acgattgtaa aacaggctgg ctaacggttc gcgagcggtt
     1981 tgccagtcca atccatgctt tacgccatgc gccagacgcg tcaccagcgc cgcgcttttt
     2041 accagcccct gagcatcgat aactgcgtca tagttctcta cttgtagcgc ttcacgaaac
     2101 gctttgcgtt cagcttttat gggggccgag aaccaggctt tacgccagcg acgtattgcc
     2161 acaggaataa ctcgctcaac ggcagcgtgc caggaaggaa tctgtgcgaa cccttcttcc
     2221 accacccagt caaacttaat ccctgggatt gcctgctggg catcagtgag tgcgggcaac
     2281 gtatggagaa catcgcccat cgacgatgtt ttaacgatca aaacccgcat ccgtcagact
     2341 tcctcttgta acaatagcgc gttgagttct tccagtacgc gctggggagt aatgtcgatc
     2401 aagctctggt gataaccctc cgcagcgtca cctttacgca ctttgtgata gccggtaatc
     2461 agacggatca cgcgcgcttt atgggatagc ggcggtgtga agtccgggct acttggacca
     2521 tacaaggcaa ccagcggacg attgagcgcc gccgcaacgt gcatcaggcc agaatcgtta
     2581 gtgacaatgg ctttacaggc tgcaatcagg ataaccgctt gatcaagctg tgtttccccc
     2641 gccaggttcc gacaccatgc ctgctgctcg gtattcaaag cggcaagaat ctcattgccc
     2701 gcctcatgat ctttcgccga gccaaacaga accacctgat aaccttcatc aatcagctgc
     2761 tttgccagct cagcatagtg gtagtgtggc cagcgttttg ccggaccaaa ctccgccccc
     2821 gggcaaaaac caatcatcgg acgttctgat gaaagcgaaa attgattaca ggtatatgat
     2881 ttttcacctt cgctcacctg caactgcggc cataacaatg gctgcggcag atcttgtgcg
     2941 gtacgcatca cgcctttgtc ataggccagc gcgacatagc gttccaccat tagcggccag
     3001 gcttctttat cgagcacgcg tacatcgttg agtaaaccgt agcgcatctc gccgcgccag
     3061 ccggtgcgat gaggaatacc cgcgaagaaa ggcactaatg cggatttgaa ggagttgggt
     3121 aagacgtagg cgcggtcgta gcgcttttca cgcaggctat gacccagttt gcggcgttcg
     3181 ccgatttcca gcgctccgtg accgagaggc atagggatag cttcgttaac ttccggcatc
     3241 cgcgataata atggacggca ccatgccggt gccatcacat cgattatcgc ctggggatag
     3301 cgcgcctgga gcgtgcgata gagactttgc gacatcatca tgtcgccaac ccaagacggg
     3361 ccgatcacca gtattttcat gcagagctct tatgcgtcgc gattcagcca ggccatgtat
     3421 tccgttacac cttcagcaac ggttttgaat ggtttgtcgt aacccgccgc gcgcagattt
     3481 gtcagatctg cctgagtgaa cgcctggtag cggcctttca gcttatccgg gaacggaatg
     3541 tattcgatct ggcctttctt gtgataagcc agcgtagcat ctgctactgc ctggaaggat
     3601 tctgcacgac cggtgccgag gttgaagata ccggaaacgc cattttccag gaaccacagg
     3661 tttacatctg ccacgtcgcc cacatagacg aagtcgcgtt tgaagttctc gctaccttca
     3721 aacagcttcg gtgattcacc gttgttaagc tgagtgttga gatggaacgc gacgctcgcc
     3781 atgctgcctt tatggccttc acgcggtcca taaacgttga aataacggaa gccaacaatc
     3841 tgcgagttcg cttcaggcag gatttggcga acatattcat caaacaggaa ttttgagtaa
     3901 ccgtagacgt tcaacggttt ttcgtactcg cgggattcaa taaagtcgga ggtgcgtccg
     3961 ccgtaggtgg ctgcggaaga agcgtacagg aacgggattt cgcgctccag gcagtagtgc
     4021 agcagctcct tggagtattg atagttgtta tccatcatat acttgccgtc ccactcggtg
     4081 gtggaagagc atgcgccttc gtggaaaatc gcttcgacat cgccgaactc ttcgccagcc
     4141 ataatctgga tcaggaagtc ttccttatcc atatagtccg cgatatccag atccaccagg
     4201 ttcacaaact tggtgccgtc tttcaggttg tccaccacca gaatatcggt gatgccttta
     4261 tcattcaggg ccttaacgat gttgctgccg ataaagcccg cgccgccggt aacgatgatc
     4321 ataactgtaa ccttcgaatt atggagtcag agacaatctc agacacgaat acttctatca
     4381 tatcactaca cggcgttggc ttcagccatt cgccaaaagt gagattgatg gacgttattt
     4441 atcctgaatt ttgcagaagt gttaacgcgt catctcgtcg cgacctataa gtttgggtaa
     4501 tatgtgctgg aatttgccct gtctggagaa tcgcaatgcg tggagaattt tatcagcagt
     4561 taaccaacga tctggaaacc gcacgggcgg aagggttgtt taaagaagag cgcattatta
     4621 cgtctgcgca gcaagcagat atcactgtgg ctgatggaag ccacgtcatt aacttttgtg
     4681 ccaacaacta tctcgggctg gcgaatcatc cggatctgat tgcggcagca aaggcgggaa
     4741 tggattctca cggtttcggc atggcttcgg tgcgttttat ttgcggcact caggacagcc
     4801 ataaagagct tgaacaaaaa ctggcggcat tcctggggat ggaagatgcg attctctact
     4861 cttcctgctt tgatgctaac ggtggactgt ttgaaacgct gctgggtgcg gaagacgcca
     4921 ttatctccga cgcactgaac cacgcgtcta ttattgatgg tgtgcgtctg tgcaaagcta
     4981 aacgctatcg ctatgccaac aacgatatgc aggagctgga agcacgtctg aaagaagcgc
     5041 gtgaagccgg tgcgcgtcat gtgctgattg ccaccgatgg tgtgttctca atggacggcg
     5101 tgattgccaa cctgaaaggc gtttgcgatc tggcagataa atacgatgcc ctggtgatgg
     5161 tggatgactc ccacgcggtc ggttttgtcg gtgaaaatgg tcgtggttcc catgaatact
     5221 gcgatgtgat gggccgggtc gatattatca ccggtacgct tggtaaagcg ctgggcgggg
     5281 cttctggtgg ttataccgcg gcgcgcaaag aggtggttga gtggctgcgc cagcgttctc
     5341 gtccgtacct gttctccaac tcgctggcac cggccattgt tgccgcgtcc atcaaagtac
     5401 tggagatggt cgaagcgggc agcgaactgc gtgaccgtct gtgggcgaac gcgcgccagt
     5461 tccgtgagca aatgtcggcg gcgggcttta ccctggcggg agccgatcat gccattattc
     5521 cggtcatgct tggcgatgca gtagtggcgc agaaatttgc ccgtgagctg caaaaagagg
     5581 gcatttacgt caccggtttc ttctatccgg tcgttccgaa aggtcaggcg cgtattcgta
     5641 cccagatgtc tgcggcgcat acccctgagc aaattacgcg tgcagtagaa gcattcacgc
     5701 gtattggtaa acaactgggc gttatcgcct gaggatgtga gatgaaagcg ttatccaaac
     5761 tgaaagcgga agagggcatc tggatgaccg acgttcctgt accggaactc gggcataacg
     5821 atctgctgat taaaatccgt aaaacagcca tctgcgggac tgacgttcac atctataact
     5881 gggatgagtg gtcgcaaaaa accatcccgg tgccgatggt cgtaggccat gaatatgtcg
     5941 gtgaagtggt aggtattggt caggaagtga aaggcttcaa aatcggcgat cgcgtttctg
     6001 gcgaaggcca tatcacctgc ggtcactgtc gcaactgtcg tggcggtcgt acccatctgt
     6061 gccgcaacac cattggtgtc ggtgttaacc gtccgggctg cttcgccgaa tatctggtga
     6121 ttccggcgtt caacgctttc aaaatccccg acaatatctc cgacgacctg gcttccattt
     6181 ttgatccctt cggtaacgcc gtgcataccg cgctgtcgtt cgatctggtg ggcgaagatg
     6241 tgctggtttc tggtgcaggt ccgataggta ttatggctgc ggcggtggcg aaacacgttg
     6301 gtgcacgcaa tgtggtgatc accgatgtta acgaataccg ccttgagctg gcgcgcaaaa
     6361 tgggtatcac ccgtgcggtc aacgtcgcta aagaaaacct taatgatgtg atggcagaac
     6421 tgggcatgac cgaaggcttt gatgtcggtc tggaaatgtc cggtgcgccg ccagcgtttc
     6481 gtaccatgct cgacaccatg aaccacggcg gtcgcattgc gatgctgggt attccaccgt
     6541 ccgatatgtc tatcgactgg accaaagtaa tctttaaagg cttgttcatt aaaggtattt
     6601 acggtcgtga gatgtttgaa acctggtaca agatggcggc gctgattcag tcaggtctgg
     6661 atctctcgcc gatcattacc catcgtttct ctattgatga tttccagaag ggctttgacg
     6721 ctatgcgttc gggccagtcc gggaaagtaa ttctgagttg ggattaacac gaacaagggc
     6781 tggtattcca gcccttttgc ctgaggataa tccgttaaat atgtaaaatc ctgtcagtgt
     6841 aataaagata tcgtaattgc gctgatctct tatatagctg ctctcattat ctctctaccc
     6901 tgaag
//
LOCUS       DAFAVF010000192         6831 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFAVF010000192 DAFAVF010000000
VERSION     DAFAVF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.194"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            496..1485
                     /gene="araF"
                     /locus_tag="LGK42_004003"
     CDS             496..1485
                     /gene="araF"
                     /locus_tag="LGK42_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBK2660184.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1555..3069
                     /gene="araG"
                     /locus_tag="LGK42_004004"
     CDS             1555..3069
                     /gene="araG"
                     /locus_tag="LGK42_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBK2660185.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3084..4070
                     /gene="araH"
                     /locus_tag="LGK42_004005"
     CDS             3084..4070
                     /gene="araH"
                     /locus_tag="LGK42_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBK2660186.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4237..5037
                     /gene="otsB"
                     /locus_tag="LGK42_004006"
     CDS             4237..5037
                     /gene="otsB"
                     /locus_tag="LGK42_004006"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBK2660187.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5012..6436
                     /gene="otsA"
                     /locus_tag="LGK42_004007"
     CDS             5012..6436
                     /gene="otsA"
                     /locus_tag="LGK42_004007"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBK2660188.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6443..>6831)
                     /gene="uspC"
                     /locus_tag="LGK42_004008"
     CDS             complement(6443..>6831)
                     /gene="uspC"
                     /locus_tag="LGK42_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBK2660189.1"
                     /translation="ESQQLLAKAVSIARPVKGHISLITLASDPEMYNQLAAPMLEDLR
                     SVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHHFDLVICGNHNHSFF
                     SRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1712 a         1600 c         1789 g         1730 t
ORIGIN      
        1 tataaaacgt tttttcattt ttgcgagatg gctctcatta tacgtgttct gttaattaat
       61 gagcacagtg ataattaatg gcgaaatgaa tgacgtgcat tttccacatc tttgagttgc
      121 ggttattaca ccatttcaaa aactcaacgc caggtaatgc ggcctattga ctggttaaaa
      181 agaagacatc ccgcatgggt accaaagaca acaaggattt ccaggctaat cttatggatt
      241 aatctgctgt gcattcgaca atttgtctga caaattggct ttcccttatg tcttttcccg
      301 ctaaatttat gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa
      361 ttaatttctc gctaaaacta tgtcaacacg gtcacttatc ttttagataa aaggtaatgc
      421 tttgttttcc gattaattta acgaaagtca ttcgtttttg ccctacacaa aacgacacta
      481 aagctggaga gaaccatgca caaatttact aaagccctgg cagccattgg tctggcagcc
      541 gttatgtcac aatccgctat ggcggagaac ctgaagctcg gttttctggt gaagcaaccg
      601 gaagagccgt ggttccagac cgaatggaag tttgccgata aagccgggaa ggatttaggg
      661 tttgaggtta ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc gatcgacagc
      721 ctggctgcca gtggcgcaaa aggtttcgtt atttgtactc cggaccccaa actcggctct
      781 gccatcgtcg cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt
      841 gttaacgcca aaggtaagcc aatggatacc gttccgctgg tgatgatggc ggcgactaaa
      901 attggcgaac gtcagggcca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc
      961 aaagaaagcg cggtgatggc gattaccgcc aacgaactgg ataccgcccg ccgccgtact
     1021 acgggatcta tggatgcgct gaaagcggcc ggattcccgg aaaaacaaat ttatcaggta
     1081 cctaccaaat ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcaa
     1141 catccggaag ttaaacattg gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc
     1201 gtacgcgcga cggaaggtca gggctttaaa gcggccgata tcatcggcat tggcattaac
     1261 ggtgtggatg cggtgagcga actgtctaaa gcacaggcaa ccggcttcta cggttccctg
     1321 ctgccaagcc cggacgtaca tggctataaa tccagcgaaa tgctttacaa ctgggtagca
     1381 aaagacgttg aaccgccaaa atttaccgaa gttaccgacg tggtactgat cacgcgtgac
     1441 aactttaaag aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt
     1501 cccctctgca tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa
     1561 cagtctaccc cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg
     1621 ctgacggata ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat
     1681 ggcgcaggaa aatcaactct cttaaaaatc ctcagcggca actatgcgcc aaccacgggt
     1741 tctgtagtga ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaatgcg
     1801 ggtgtggcga ttatttacca ggaactgcat ctcgtgccgg aaatgaccgt cgcggaaaac
     1861 atctatctcg gccagctgcc gcataaaggc ggcattgtga atcgctcatt gctgaattat
     1921 gaggcgggtt tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa
     1981 tatctctcca ttggtcagtg gcagatggtt gaaatcgcca aggcgctggc gcgtaacgct
     2041 aaaattatcg cctttgatga gccaaccagc tccctttctg cccgcgaaat cgacaatctt
     2101 ttccgcgtta ttcgtgaact gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt
     2161 atggaagaaa tatttgccct cagcgatgcc atcactgtct ttaaagatgg acgttatgtc
     2221 aaaaccttta ccgatatgca gcaggttgac cacgacgcgc tggtgcaggc gatggtcggg
     2281 cgcgacattg gcgatatcta cggctggcaa cctcgtagtt atggcgagga gcgcctgcgt
     2341 cttgatgctg tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtggt
     2401 gaaattgttg ggctgtttgg tctggtagga gcggggcgta gcgaattaat gaaaggcttg
     2461 tttggcggga cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt
     2521 cgtaaaccga gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg
     2581 gaaggcatta ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaaa
     2641 catgtgctcg gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac
     2701 attcgttcgc tcaacatcaa aacgccgggc gctgagcaac tgatcatgaa tctctcaggc
     2761 ggaaatcagc aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg
     2821 ctggatgaac ctacgcgcgg cattgatgtt ggtgctaagc atgaaattta caacgtgatt
     2881 tatgcgctgg cggcgcaggg tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc
     2941 ctcggcgttg ccgaccggat tgtggtgatg cgggaaggtg aaatcgccgg tgaattgtta
     3001 cacgagcagg cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagccaggca
     3061 gttgcctgag taaggagagt atgatgtctt ctgtttctac atcggggtct ggcgcaccta
     3121 agtcgtcatt cagcttcggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg
     3181 tgctctttat cgcctgtgcc atttttgtcc caaattttgc caccttcatt aatatgaaag
     3241 ggttgggcct ggcaatttcc atgtcgggga tggtggcgtg tggcatgttg ttctgccttg
     3301 cttccggtga ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg
     3361 cggtggttat caacctgact gaaagcctgt ggattggcgt ggcagcgggg ttgctgcttg
     3421 gcattctctg tggcctggtc aatggctttg ttatcgccaa actgaaaata aatgctctga
     3481 tcacaacact ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta
     3541 aagcggtcgg tatcgaagat gaaagcttct ttgcccttgg ttacgctaac tggttcggtc
     3601 tgcctgcgcc aatctggctc accgtcgcgt gtctgattat ctttggtttg ttgctgaata
     3661 aaaccacctt tggtcgtaac accctggcga ttggcgggaa cgaagaggct gcgcgtctgg
     3721 cgggtgtacc ggttgttcgc accaaaatta ttatctttgt tctctctggc ctggtatctg
     3781 cgatagccgg aattattctg gcttcacgta tgactagtgg gcagccaatg acgtcgattg
     3841 gttatgagct tattgttatc tccgcctgcg ttttaggtgg cgtttctctg aaaggtggca
     3901 tcggaaaaat ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca
     3961 tgaacctgct taatatttct cctttcgcgc agtacgtggt tcgcggctta atcctgctgg
     4021 cagcggtgat cttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc
     4081 cacaacaatt taacgttttt tcccaccaca gccagccgcc acaacggttg gctgttcttc
     4141 attgcaaatg gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg
     4201 cgcaaaacaa taagaaaaga gaaggaggag agccgggtga cagaaccgtt aaccgaaacc
     4261 cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc
     4321 aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg
     4381 gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac
     4441 gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac
     4501 atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg
     4561 caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct
     4621 tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa
     4681 cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc
     4741 aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt
     4801 atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc
     4861 gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg
     4921 cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa
     4981 caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg
     5041 gattgcacca ccagacgagc acgccgccag tgccggtggc cttgccgttg gcatactggg
     5101 ggcactgaaa gccgcaggcg gactgtggtt tggctggagt ggtgaaacag ggaatgagga
     5161 tcagccgcta aaaaaggtga aaaaaggtaa cattacgtgg gcctctttta acctcagcga
     5221 acaggacctt gacgaatact acaaccaatt ctccaatgcc gttctctggc ccgcttttca
     5281 ttatcggctc gatctggtgc aatttcagcg tcctgcctgg gacggctatc tacgcgtaaa
     5341 tgcgttgctg gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca
     5401 cgattatcac ctgttgccat ttgcgcatga attacgcaaa cggggagtga ataatcgcat
     5461 tggtttcttt ctgcatattc ctttcccgac accggaaatc ttcaacgcgc tgccgacata
     5521 tgacaccttg cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga
     5581 tcgtctggcg ttcctggatt gtctttctaa cctgacccgc gtcacgacac gtagcgcaaa
     5641 aagccataca gcctggggca aagcatttcg aacagaagtc tacccgatcg gcattgaacc
     5701 gaaagaaata gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc
     5761 ggaactgaaa aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt
     5821 gccagagcgt tttctcgcct atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa
     5881 aattcgttat acccagattg caccaacgtc gcgtggtgat gtgcaagcct atcaggatat
     5941 tcgtcatcag ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg
     6001 gacgccgctt tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg
     6061 ctactctgac gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga
     6121 gtatgttgct gctcaggacc cagccaaccc gggcgttctt gttctttcgc aatttgcggg
     6181 agcggcaaac gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc
     6241 agctgcgctg gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga
     6301 aatgctggac gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga
     6361 cctaaagcag atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt
     6421 tccaaagctt gcgtaggagc ggttaatcac ccgtaagtgg aactaacagc acatcgacct
     6481 cacttgaggc aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt
     6541 gattaccgca aatcaccaaa tcgaaatgat gtttgtgaca tacttccaga atatgttcgc
     6601 ttaactcacc gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact
     6661 tatcaagaaa gctttgcgtt tcttcttgca tcacgctgcg taaatcttca agcatcggcg
     6721 cggctaattg attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct
     6781 ttactggcct ggcgatagat accgcttttg ccagcagttg ctgactttcc g
//
LOCUS       DAFAVF010000193         6773 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFAVF010000193 DAFAVF010000000
VERSION     DAFAVF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.195"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            262..780
                     /locus_tag="LGK42_004009"
     CDS             262..780
                     /locus_tag="LGK42_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBK2660190.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            990..3131
                     /locus_tag="LGK42_004010"
     CDS             990..3131
                     /locus_tag="LGK42_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBK2660191.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDSP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3207..6408
                     /locus_tag="LGK42_004011"
                     /pseudo
     CDS             3207..6408
                     /locus_tag="LGK42_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1597 a         1725 c         2150 g         1301 t
ORIGIN      
        1 ctttggccat tttatggagg caatacggac tttgaacagt tgggtatggt tatccagata
       61 tatattatta agttattgct gaataagaga tttaatgatt ttaaaaatag tttttacgac
      121 aaccgtaaat tctagctcgg tttgtatgta aaaaatactg aaaaatgatg cggttccgtg
      181 aagtgagctg catctgcctg tctgggatta acggcaggtc attgtaagta gtaattaacc
      241 acgttaacaa ggagtttaca aatgccaaca ccgtgttata tctccatcac tggtcagacc
      301 cagggaaata ttactgcagg tgcatttacc gctgattctg tcggcaacat ctatgtgcag
      361 gggcacgaag acgagatgct ggtgcaggaa ttcctgcata acgttactgt cccgaccgat
      421 cctcagtccg gtcagcctgc gggtcagcgt gctcacaagc ctttcatttt taccgtggcg
      481 ctgaacaaag cggttccgct gatgtacaac gcactggcct ccggcgaaat gctgccaacc
      541 actgaacttc actggtggcg tacctctgtg gaaggcaaac aggagcatta tttcactacc
      601 cgcctgactg attcgacgat tgtcgatatg aaactgcata tgccgcattg ccaggacccg
      661 gcgaaacgcg agttcaccca gttgctcgaa gtttcactgg cctaccgcaa aattgaatgg
      721 gaacacgtca aatctggcac ctccggtgct gacgactggc gcgcaccgct ggaagcataa
      781 gttaagccaa cagcatccgg ctatcaggcc ggatgctgtt tttgtattac gccgtagaaa
      841 gaaaggttgt cagtaagggg ttacgttcac gggagatggc ggctgccgga aacttcggcg
      901 gtagcgtgcc tgaacgcaag cgtaacactg tactgtcaca gccgctttaa cagcgaactg
      961 tctgtttttc cttaaccccg gaggttgcta tgtcaaccgg attacgtttc acgctggaag
     1021 tggacggcct gccaccggat gcttttgcgg tggtctcctt tcatctgaac cagtcactct
     1081 cttcgctttt ttccctcgat ctctctctgg tcagccagca gtttctctcc cttgaattcc
     1141 agcagatcct cgacaaaatg gcctacctga cgatatggca gggcgatgac gtacagcgcc
     1201 gggtgaaagg tatggtgacc tggtttgaac tgggggagaa cgacaaaaac cagatgctgt
     1261 acagcatgaa ggtgtgccca ccgctgtggc gcacagggct gcgccagaac ttccgtatct
     1321 tccagaatga ggacatcgaa agcatcctcg gcacgatatt gcaggaaaac ggggtgaccg
     1381 agtggagccc gctgttcagc gagccacatc cttcccgtga gttttgtgtc cagtacggtg
     1441 agactgatta cgatttcctg tgccggatgg cggcggagga aggcatcttc ttttatgagg
     1501 agcacgcgca aaaaagtacc gaccagagcc tggtcctgtg cgataccgtg cgttatctgc
     1561 cggagtcctt tgagatcccc tggaatccga acacccgtac cgaggtgagc accctctgca
     1621 tcagccagtt tcgctacagc gcacaaatcc gcccttcttc cgtggtgacc aaagactaca
     1681 cctttaaacg acccggctgg gcagggcgtt ttgatcagga aggccagtac caggattacc
     1741 agcgcacaca gtatgaagtg tatgactacc ccggacgttt caagggtgcc cacgggcaga
     1801 actttgcccg ctggcagatg gatggctggc gcaacaacgc agaagtggcg cgcggaacaa
     1861 gccgttcgcc ggagatatgg ccgggacggc gaattgtgct gacggggcat ccgcaggcga
     1921 acctgaaccg ggaatggcag gtggtggcga gcgatcttca cggcgaacag ccgcaggcgg
     1981 taccgggacg caggggttca ggcaccacgc tggataacca ctttgcggta atcccggcag
     2041 accggacatg gcggccacag ccgttgctga aaccgctggt ggacggcccg cagagcgccg
     2101 tcgtgacggg accggcaggc gaggaaatct tctgtgatga acatggccgc gtgcgggtga
     2161 aatttaactg ggaccgctat aacccgtcaa accaggaaag ttcatgctgg atccgtgtgg
     2221 cacaggcgtg ggcaggcacc ggctttggta acctggcgat accgcgtgtg ggtcaggagg
     2281 tcattgtgga cttcctcaac ggcgatccgg accagccgat cattatgggg cgtacctacc
     2341 accaggaaaa ccgcaccccc ggcagcctgc cggggacgaa gacgcagatg acgatccgct
     2401 cgaaaaccta taagggcagc gggtttaatg aactgaagtt tgacgatgcg acagggaaag
     2461 aacaggtcta catccacgcg cagaagaaca tgaacaccga ggtgctgaat aaccgcacca
     2521 ctgatgtgat aaacaaccat gccgaaaaaa taggtaacaa ccaggcgatc accgttacca
     2581 ataaccagat ccagaacatt ggcgttaatc agatacagac ggttggtgtc aaccaggtgg
     2641 aaacggtggg cagtaaccag attatcaaag tgggatcaaa ccaggttgaa aaggtgggga
     2701 tcattcgtgc gctgacggtg ggtgtggctt accagacgac ggtaggcggc attatgaata
     2761 cctcggtggc gttgttgcag tcctcacagg tagggctgca taaatcactg atggtgggaa
     2821 tgggctacag cgtcaatgtg gggaataacg tcaccttctc ggtgggcaag acaatgaagg
     2881 aaaacaccgg acaaacagca gtttattctg ccggtgagca tcttgaactc tgctgtggta
     2941 aggcaaggct ggtgctgacg aaggacggaa gcatatttct caatggtacg cacattcatc
     3001 tggaagggga gtcggatgtg aacggtgatt cgccagtgat taactggaac tgtggtgcca
     3061 cacaacctgt accggatgcg cctgtgccga aagatttacc ccccggaatg ccggatatgc
     3121 ggcaattttg agtcaggtgc aaaaaattaa aacggaactg gtgcagtgac cagataaacc
     3181 atattaaaag cagaaggaat tgattcatgg gcggaaaacc ggcggcgcgg cagggtgata
     3241 tgacccgcaa gggactggat attgtgcagg gttcagcggg ggtgctgata ggtgcgccga
     3301 cgggagtggc ctgttcggta tgtccgggag ggattaccta tgctaacccg gtgaacccgc
     3361 tgctgggtgc gaaggtgctg ccgggcgaga cggaccttgc gctgcccggc ccgctgccgt
     3421 ttattctttc ccgcgcctac agcagctacc ggaccagaac acccgcgccg gtgggggtgt
     3481 ttggtcccgg ctggaaagcg cccttcgata tccgcttaca gatacgcgat gaaggcctga
     3541 tactcaacga caacggcggc aggagcattc actttgagcc gctgtttccc ggcgagataa
     3601 gctacagccg cagcgagtca ctctggctgg cgcggggcgg ggtggcggag cagcacagtt
     3661 cgcagccgct aagcgcactc tggcaggtgc tgccggaaga tgttcgcctg agtccgcatg
     3721 tgtacctggc gacaaacagc cttcaggggc cgtggtggat actcagctgg ccggagcggg
     3781 tgccgggggc ggacgaggtg ctgccgccgg agccgcccgc ataccgggtg ctgacgggcg
     3841 tggtggacgg cttcgggcgg acgctgactt tccaccgggc ggcggagggt gacgtggcgg
     3901 gcgcggtgac gggggtgacg gacggcgcgg gccgctgttt tcacctggtg ctgaccacac
     3961 aggcgcagcg ggcggaagcc ttccgtaaac agcgcgccac gtctttatct tcccctgccg
     4021 gtccccgttc tgcttcctct tcttctgctt tccccgacac gctgcccgcc ggtacagaat
     4081 acggtgccga taacggtatc cggctggagg cggtgtggct gacacacgac ccggcatacc
     4141 cggatgaaca gccgactgcg ccgctggcgc gctacacgta cacggccagc ggagaactgc
     4201 gggcggtgta tgaccgcagc gggacgcagg ttcgcgggtt tacttatgat gcggagcacg
     4261 ccgggcggat ggtggcacac cattatgcgg ggaggccgga gagccgctac cggtatgatg
     4321 ataccggtcg ggtgacggag caggtcaacc cggaggggct ggactaccgc tttgagtacg
     4381 gagagcgccg ggtaatcatc acggacagcc tgaaccggcg ggaggtgctg tacacggagg
     4441 gcgaaggcgg cctgaagcgg gtggtgaaga aggagcatgc ggacgggagc atcacccgca
     4501 gcgagtatga tgaggcgggg aggctgaagg cacagacgga tgcggcgggg cggcggacgg
     4561 agtacagcct gcatatggcg tcgggtgcgg tgacggcggt gacggggccg gacggcagga
     4621 cggtacggta tggctataac agccagcggc aggtgacgtc ggtgacgtac ccggacgggc
     4681 ttcgcagcag ccgggagtat gatgagaagg gaaggctgac ggcggagacc tcgcgcagcg
     4741 gtgagacaac gcggtacagc tatgatgacc cggcgagtga gctgccgaca gggatacagg
     4801 acgcgacggg cagtacaaaa cagatggcat ggagccgtta cggtcaactg ctggccttca
     4861 cggactgctc ggggtacacg acgcggtatg agtacgaccg gtacggtcaa caaatcgccg
     4921 ttcaccggga ggaaggcatc agcacttaca gcagttataa cccgcgtggg caactggtca
     4981 gtcagaagga tgcgcagggg cgtgaaatcc gttatgagta cagcgccgca ggcgacctga
     5041 cagcaacgat atcaccggac ggtaaacgca gcaccatcgc atatgataaa cgcgggcgtc
     5101 cggtgagtgt gacggaaggc ggtcttacgc gcagcatggg gtatgacgct gccgggcgca
     5161 tcaccgtgct gaccaacgag aacggcagcc agtccacgtt ccggtatgac ccggtggaca
     5221 ggctgactga acagcgcggt tttgacggcc ggacgcaacg ttaccagtat gacctgaccg
     5281 gaaaactcac gcagagtgaa gatgaggggc ttatcaccct ctggcattac gatgcgtcgg
     5341 accgtatcac ccggcgcacg gtgaacggtg aaccggcgga gcagtggcag tatgacgacc
     5401 acggctggct gacagaaatc agccacctga gtgaaggcca ccgtgtggcg gtccactacg
     5461 gctatgacga taaaggccgt ctgacgggcg aacggcagac tgacggagaa gacggaccgc
     5521 atcccgacgg gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc
     5581 cagcaccgcc tggtgttcca cacgcggata cagcatggcg agccactggt cgagagccgc
     5641 tacctctacg acccgctggg acggcgaatg gcgaaacggg tatggcggcg ggagcgtgac
     5701 ctgacggggt ggatgtcgct gtcgcgtaaa ccggaggaga catggtacgg ctgggacggc
     5761 gacaggctga cgacggtaca gaccgacacc acgcgtatcc agacggtata ccagccgggg
     5821 agcttcgcgc cgctcatccg catcgaaaca gacaacggtg aacgggagaa agcgcagtgc
     5881 cgcagcctgg cggagaagat acagcaggaa gggagcgagg acgggcacgg cgtggtattt
     5941 ccggctgaac tggtggggct gctggacagg ctggaggggg aaatccgggc aaactgcgtg
     6001 agcagtgaaa gccgccagtg gcttgcgcag tgcgggctga cggtggagcg gctggcggca
     6061 cagatagagc cggtttattt gcctgagcga aaaatacatc tttatcactg tgaccaccgg
     6121 ggcctgccgc tggcgcttat cagtgaagac ggcaatacgg cgtggagcgc ggaatatgat
     6181 gaatggggca accagcttaa tgaggagaac ccgcatcacc tgcaccagcc gtaccggctg
     6241 ccggggcagc agtatgataa ggagtcgggg ctgtactata accgtcaccg gtactacgat
     6301 ccgttgcagg ggcggtatat caccccggac ccgattgggt tgagaggtgg atggaatatg
     6361 tatcagtatc cgttgaatcc catacaagtg atagacccaa tggggttaga tgcgattgag
     6421 aatatgacat caggtggact aatttatgcc gtatctggtg tacctggatt gattgctgca
     6481 aacagcatta ctaacagtgc ttaccagttc ggttatgata tggatgctat tgttggcgga
     6541 gctcataatg gggccgccga tgcaatgaga cattgttact tgatgtgtcg aatgactaag
     6601 acatttggat caacaatagc tgacgtgata ggtaaaaatc atgaggcggc agggaataga
     6661 caaggtcagc cagctaaaga aagaatcatg gatcttaaaa ataacactgt cggtattgct
     6721 tgtggcgatt tttctgccaa atgtagcgat gcatgtattg aaaaatataa cac
//
LOCUS       DAFAVF010000194         6760 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFAVF010000194 DAFAVF010000000
VERSION     DAFAVF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.196"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1008
                     /gene="dusA"
                     /locus_tag="LGK42_004012"
     CDS             <1..1008
                     /gene="dusA"
                     /locus_tag="LGK42_004012"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBK2660192.1"
                     /translation="NQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLL
                     YTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNV
                     GCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDF
                     INTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGG
                     IKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYI
                     ERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADK
                     R"
     gene            1142..1384
                     /gene="pspG"
                     /locus_tag="LGK42_004013"
     CDS             1142..1384
                     /gene="pspG"
                     /locus_tag="LGK42_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBK2660193.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1550..2533)
                     /locus_tag="LGK42_004014"
     CDS             complement(1550..2533)
                     /locus_tag="LGK42_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBK2660194.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGCKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2616..4031
                     /gene="dnaB"
                     /locus_tag="LGK42_004015"
     CDS             2616..4031
                     /gene="dnaB"
                     /locus_tag="LGK42_004015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK2660195.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4084..5163
                     /gene="alr"
                     /locus_tag="LGK42_004016"
     CDS             4084..5163
                     /gene="alr"
                     /locus_tag="LGK42_004016"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK2660196.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5416..6609
                     /gene="tyrB"
                     /locus_tag="LGK42_004017"
     CDS             5416..6609
                     /gene="tyrB"
                     /locus_tag="LGK42_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBK2660197.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1566 a         1694 c         1828 g         1672 t
ORIGIN      
        1 aaccaaacca gcgcaatgcc tgaaaaaact gacgttcact ggagtggtcg gtttagcgtt
       61 gcaccaatgc tcgactggac ggacagacat tgccgctatt tcttgcgtct gctttcccgc
      121 aatacgttgc tgtataccga aatggtgacc acaggggcga ttattcacgg taaaggtgat
      181 tacctggcgt acagtgaaga agaacatccg gtagcgttgc aactcggcgg tagcgatccg
      241 gcggcgctgg cgcagtgtgc aaagctggca gaagcgcgcg gatatgatga gatcaacctg
      301 aatgtcggct gcccgtctga ccgggtgcag aacggcatgt ttggtgcgtg tctgatgggt
      361 aatgcgcagc tggttgccga ctgcgtgaaa gcgatgcgcg atgtggtgtc gattccagtg
      421 acggtgaaaa cgcgtattgg catcgatgac caggacagct atgaatttct ctgcgatttc
      481 atcaacaccg tttccggcaa aggcgagtgt gagatgttta tcatccacgc acgtaaagcc
      541 tggctttcgg ggttaagccc gaaagaaaac cgtgaaatcc cgccgctcga ttatccgcgt
      601 gtgtatcaac tgaagcgtga ctttccgcat ctgacaatgt cgattaacgg tggtatcaag
      661 tcgctggaag aggccaaagc acacctgcaa catatggatg gcgtgatggt cgggcgcgag
      721 gcgtatcaga atccgggtat tctggcggcg gtagaccggg agatctttgg ttcctcggat
      781 accgatgccg atccggtggc ggtagtgcgc gccatgtatc cgtacattga gcgtgaactc
      841 agccagggga cgtatctcgg ccatattacc cggcatatgt tgggcttgtt ccagggtatt
      901 cctggcgcgc ggcagtggcg gcgttattta agtgaaaatg cccataaagc gggtgcagac
      961 attaatgtgc tggaacacgc gctcaaactg gtggcggata agcgttaact tttcaccaaa
     1021 aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg cgttttgctg ttaaatcaat
     1081 agattatttt tggcatgatt cttgtaatgc cagcaagaga tttcatattt gggagagcat
     1141 catgctggaa ctactttttg tgtttggctt ttttgtcatg ctgatggtca ccggcgtttc
     1201 gttgctgggc attatcgccg cgctggttgt ggcgacggcc attatgttcc tcggcggtat
     1261 gctggcattg atgattaagt tgctgccgtg gttactactg gcgattgcgg tggtgtgggt
     1321 tattaaggcg attaaagcac caaaagtgcc gaaatatcag cgttatgacc gctggcgtta
     1381 ctaagggatt gtgcggatga tcacaaccta aggttttatc cttagaacaa aataggaatt
     1441 gataatcaaa tctgtcacta ttgcgcctct aacagattca tcgtgctgta ccctacatac
     1501 agccgaacta taaaaagaaa gggcttccca ggtggaagcc ctatttcttt tatggaatca
     1561 gcaggctgga accttgcgtc gcccggcttt ccagaatctc atgcgcacgc tgcgcatcct
     1621 tcagcggata tttctgctgc tcggcgacat cgaccttaat cacaccgctg gcaatcaaag
     1681 agaacagttc attactggcc tcggttaatt cctcccgcgt ggtgatatag ccttgcaggg
     1741 aagggcgtgt cacatacaac gagccttttt gattgagaat gcctaagttc acaccggtaa
     1801 ccgcacctga tgagttgcca aaactgacca ttaagccgcg gcgttgcagg caatccagcg
     1861 accgttccca ggtgtctctg cccacggaat cgtacactac gcgcactttc ttacagccgg
     1921 tgatctcttt caatcgctcg accagattct cttcacgata gttaataacc tgccacgcgc
     1981 cggcttttag cgcgctctgc gctttttgcg cggttcctac ggtgccgata agtttcgcgc
     2041 ccagggcttt tgcccactgg caggcaatta agccaacgcc gccagccgct gcgtggaaca
     2101 ggaactgttc atcgggttta atttcatagg ttttgcgcag cagataataa accgttaagc
     2161 ctttcaggaa agatgctgca gcttgttcaa aagaaattgc cgcaggcaga atcgccgctt
     2221 tatccgcatt aatgttatgc acagagctgt aagcgcctaa cgccgactgc gcatagacta
     2281 cacgatcgcc tgccttaata tgctttacac cactgccgac tttactcacg atgcctgccg
     2341 cttcggtacc taatccgctg ggtagtgatg gcggcgggta aaggccgctg cggatatacg
     2401 tgtcgataaa attgatgccg atggctttat tttcgacctg gatttcattc tctgccggat
     2461 cggcaggagt gaactctacg gcttgaagta cttccgggcc accgtgcttg tgaaattcaa
     2521 ttcgtgttgc catgtgtcct ccagaacgta tcgtcagggt ctgcttcata tggtaaagtt
     2581 tcgacccatt ctttatctcg gtaactccat tcactatggc aggaaataaa cccttcaaca
     2641 aacagcaggc tgaaccccgc gaacgcgatc cacaagttgc cgggctgaaa gtgcctccgc
     2701 actcgatcga agcggagcag tcggtgttgg gcggtttaat gctggataac gaacgctggg
     2761 acgatgtagc cgagcgtgtg gtggcagacg atttttacac ccgcccacac cgccatatct
     2821 ttactgaaat ggcgcgtttg caggaaagcg gtagtcctat cgatctgatt acccttgcgg
     2881 aatcgctgga acgccagggg caactcgata gcgtcggtgg ttttgcttat ctggcagagc
     2941 tgtcaaaaaa tacgccaagt gcggcgaaca tcagtgccta tgcggacatc gtgcgtgaac
     3001 gtgccgttgt ccgtgagatg atctcggttg cgaatgagat tgctgaagcc ggttttgatc
     3061 cgcaggggcg taccagcgaa gatctgctgg accttgctga atcccgcgtc tttaaaattg
     3121 ccgaaagtcg tgcaaacaaa gacgaagggc cgaagaacat cgccgatgtg ctcgacgcaa
     3181 ccgtggcgcg tattgagcag ttgtttcagc agccacacga tggcgttacc ggagtaaaca
     3241 ccggttatga cgatctcaac aaaaaaaccg ctggcttgca gccgtcggat ttgatcatcg
     3301 tcgccgcgcg tccgtcgatg ggtaaaacaa catttgcgat gaacctcgtc gaaaacgcgg
     3361 cgatgttgca ggataaaccg gtacttatct tctcgctgga gatgccttca gaacagatta
     3421 tgatgcgttc tctggcgtcg ctgtcgcgcg ttgaccagac taaaatccgt accgggcagc
     3481 tcgatgatga agactgggcg cgcatttccg gcaccatggg tattttgctc gaaaaacgca
     3541 atatctatat cgatgactcc tccggcctga cgccaacgga agtgcgttcc cgcgcacgcc
     3601 gtattgcccg tgaacacggc ggcatcgggc ttatcatgat cgactacctg caactgatgc
     3661 gcgtaccggc gctttccgat aaccgtacgc tggaaattgc agaaatctcc cgctcgctga
     3721 aagcactggc gaaagaactg aacgtgccgg tggtggcgct gtcccagttg aaccgttctc
     3781 tggaacaacg tgccgacaaa cgcccggtca actccgacct gcgtgaatct ggctctatcg
     3841 agcaggatgc ggacttgatc atgtttatct atcgtgatga ggtgtatcac gaaaacagtg
     3901 atttaaaagg catcgcggaa attattatcg gtaaacaacg taacggccca atcgggacgg
     3961 tacgcctgac ctttaacggt caatggtcgc gcttcgacaa ctatgcgggg ccgcagtacg
     4021 acgacgaata ataattattt tatgaattag gtaattaaag caaacactta tcaaggaaca
     4081 caaatgcaag cggcaactgt tgtgattaac cgccgcgctc tgcgacacaa cctgcaacgt
     4141 cttcgtgaac tggcccctgc cagtaaaatg gttgcggtgg tgaaagcgaa cgcttatggt
     4201 cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg acgcctttgg cgtagcccgt
     4261 ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca aacctgtact gttactcgaa
     4321 ggcttttttg atgccagaga tctgccgacg atttctgcgc aacattttca taccgccgtg
     4381 cataacgaag aacagctggc tgcgctggaa gaggctagcc tggacgagcc ggttaccgtc
     4441 tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa ggccggaaca ggctgaggcg
     4501 ttttatcatc gcctgaccca gtgcaaaaac gttcgtcagc cggtgaatat cgtcagccat
     4561 tttgcgcgcg cggatgaacc aaaatgtggc gcaaccgaga aacaactcgc tatctttaat
     4621 accttttgcg aaggcaaacc tggtcaacgt tccattgccg catcgggtgg cattctgctg
     4681 tggccacagt cgcattttga ctgggtgcgc ccgggcatca ttctttatgg cgtctcgccg
     4741 ctggaagatc gctccaccgg tgccgatttt ggctgtcagc cagtgatgtc actaacctcc
     4801 agcctgattg ccgtgcgtga gcataaagcc ggagaacctg ttggttatgg tggaacctgg
     4861 gtaagcgaac gtgatacccg tcttggcgta gtcgcgatgg gctatggcga tggttatccg
     4921 cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc gcgaagtacc gattgtcggg
     4981 cgcgtggcga tggatatgat ctgcgtagac ttaggtccac aagcgcagga caaagccggg
     5041 gatccggtca ttttatgggg cgaaggtttg cccgtagaac gtatcgctga aatgacgaaa
     5101 gtaagcgctt acgaacttat tacgcgcctg acttcaaggg tcgcgatgaa atacgtggat
     5161 taatcgttct gtaatatttg attgtctgtg ccggatgcgg cgtgaatgcc ttatccggcc
     5221 aataaaatcc taaaaattca ataagttgat gttctttcat gctcttataa aggtcgtgcc
     5281 tctggcggat gtacgtttgt catgagtctc actctgttgc taattgccgt tcgctcctga
     5341 acatccactc gatcttcgcc ttcttccggt ttattgtgtt ttaaccacct gcccgtaaac
     5401 ctggagaacc atcgcgtgtt tcaaaaagtt gacgcctacg ctggcgaccc gattcttacg
     5461 cttatggagc gttttaaaga agaccctcgc agcgacaaag tgaatttaag tatcggtctg
     5521 tactacaacg aagacggaat tattccacaa ctgcaagccg tggcggaggc ggaagcgcgc
     5581 ctgaatgcgc agcctcatgg cgcttcgctt tatttaccga tggaagggct taacagctat
     5641 cgccatgcca ttgcgccgct gctgtttggt gcggaccatc cagtactgca acaacagcgc
     5701 gtagcaacca ttcaaaccct tggcggctca ggggcattga aagtgggcgc ggatttcctg
     5761 aaacgctact tcccggaatc aggcgtctgg gtcagcgatc ctacctggga aaaccacgta
     5821 gcaatattcg ccggggctgg attcgaagtg agtacttacc cctggtatga cgaagcgact
     5881 aacggcgtgc gctttaatga cctgttggcg acgctgaaaa cattacctgc ccgcagtatt
     5941 gtgttgctgc atccatgttg ccacaaccca acgggtgccg atctcactaa tgatcagtgg
     6001 gatgcggtga ttgaaattct caaatcccgc gagcttattc cattcctcga tattgcctat
     6061 caaggatttg gtgccggtat ggaagaggat gcctacgcta ttcgcgccat tgccagcgct
     6121 ggattacccg ctctggtgag caattcgttc tcgaaaattt tctcccttta cggcgagcgc
     6181 gtcggcggac tttctgttct gtgtgaagat gccgaagccg ctggccgcgt actggggcaa
     6241 ttgaaagcaa cagttcgccg caactactcc agcccgccga attttggtgc gcaggtggtg
     6301 gctgcagtgc tgaatgacga ggcattgaaa gccagctggc tggcggaagt agaagagatg
     6361 cgtactcgca ttctggcaat gcgtcaggaa ctggtgaagg tattaagcac agagatgcca
     6421 gaacgcaatt tcgattatct gcttaatcag cgcggcatgt tcagttatac cggtttaagt
     6481 gccgctcagg ttgaccgact acgtgaagaa tttggtgtct atctcatcgc cagcggtcgc
     6541 atgtgtgtcg ccgggttaaa tacggcaaat gtacaacgtg tggcaaaggc gtttgctgcg
     6601 gtgatgtaat gcaggaaagc aggctggagc tacccagcct gcagtgaaat taaactgtcg
     6661 tcgctttcac tctttcttta tagatgattt ttttgatgcc atcgttctac gtgagagata
     6721 ataaacgtta ttagttcttt tattgttaag cttatcctaa
//
LOCUS       DAFAVF010000195         6758 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFAVF010000195 DAFAVF010000000
VERSION     DAFAVF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.197"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..124)
                     /locus_tag="LGK42_004018"
     CDS             complement(<1..124)
                     /locus_tag="LGK42_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK2660198.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSAS"
     gene            complement(121..390)
                     /locus_tag="LGK42_004019"
     CDS             complement(121..390)
                     /locus_tag="LGK42_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660199.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            748..1893
                     /locus_tag="LGK42_004020"
                     /pseudo
     CDS             748..1893
                     /locus_tag="LGK42_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            complement(2069..2371)
                     /locus_tag="LGK42_004021"
     CDS             complement(2069..2371)
                     /locus_tag="LGK42_004021"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660200.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2458..3273)
                     /gene="sul2"
                     /locus_tag="LGK42_004022"
     CDS             complement(2458..3273)
                     /gene="sul2"
                     /locus_tag="LGK42_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBK2660201.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3603..3779
                     /locus_tag="LGK42_004023"
     CDS             3603..3779
                     /locus_tag="LGK42_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBK2660202.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(3961..4716)
                     /locus_tag="LGK42_004024"
                     /pseudo
     CDS             complement(3961..4716)
                     /locus_tag="LGK42_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(4769..5473)
                     /locus_tag="LGK42_004025"
     CDS             complement(4769..5473)
                     /locus_tag="LGK42_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBK2660203.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(5675..6463)
                     /locus_tag="LGK42_004026"
     CDS             complement(5675..6463)
                     /locus_tag="LGK42_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBK2660204.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(6594..>6758)
                     /locus_tag="LGK42_004027"
     CDS             complement(6594..>6758)
                     /locus_tag="LGK42_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DfrA family trimethoprim-resistant dihydrofolate
                     reductase"
                     /protein_id="HBK2660205.1"
                     /translation="NSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNI
                     NYTYQIWKKG"
BASE COUNT         1588 a         1854 c         1842 g         1474 t
ORIGIN      
        1 tgctggctga ataaccaatc cgttcgtcca gttcaatggc ggccatcagg ttctgctccg
       61 caatgtactt acgaatgcgc ttcctgtcct ggctggccag catggtccag aatatctcca
      121 tcatttaccg gcaaccttcc tgctcatttc atcccgccat gcagcagcgg aagattcgac
      181 catgtcatgg ggaagcacat cgccgcgctc gagttgctga cgtcctgcct caacctggtc
      241 acggaaccag gtattatgct gccgttcaac ggtctggcgc ataaaatccc gaattaactg
      301 agagccatta cggtccatgc tttttgcagc ggccataaaa gcatccttca gttcagcgtc
      361 gattcttaaa ctcatattaa cctgtgccat ttatcacctc gtcattacta cgtatataca
      421 cagtatataa cgattcagat atcgtcacaa gcgaagcgcg gaggccgcag gccggaggca
      481 ttagtggccg ccgcccgaag gggcgagacg cgtagcggct cgatgcgcag cacggcagaa
      541 cggccccgca ggggtaatgc ccagtttaat tcaacgtgac agtcacgtgg aggaaaaata
      601 tgaatgaccg acagcgtgaa caagcccgta ttcgccaggc ccggcgccgc gcgcgactca
      661 aagaggaagg cgctagcgtg acagtcacgc taacaaaaca ggaagaagca atgttacagg
      721 agctttgccg ggttcgccgt ccaggacgaa caacccatgc gtcagtgggt gttgagcttc
      781 ccgtttcagc tgcgtttcct gtttgccagc cggcccgaga tcatggggtg ggtgctgggc
      841 atcgtttacc gcgtcattgc cacgcacctg gtcaagaaag cgggccatac ccaccaagtg
      901 gccaagacgg gcgcggtcac cctgatccag cgttttggat cggcgctcaa tctgaatgtt
      961 cacttccaca tgctgtttct cgacggtgtg tatgtcgagc aatcccacgg ctcagcgcgt
     1021 ttccgctggg tcaaggcgcc gaccacccca gagctcaccc agctgacgca caccatcgcc
     1081 caccgggtgg gtcgctatct ggaacggcaa ggcctgctgg aacgggatgt cgaaaacagc
     1141 tatctggcct cggatgcggt ggatgacgac ccgatgacac ccctgctggg gcactcgatc
     1201 acttaccgta tcgctgtcgg ttcacaggcg gggcgaaagg tgttcacttt gcaaactctg
     1261 ccgaccagtg gtgatccgtt cggtgacggg attggcaagg tagccgggtc cagcctgcac
     1321 gccggcgtgg cggccagggc cgatgaacgc aagaagctcg aacggctgtg ccggtacatc
     1381 agccgcccgg cggtatccga gaagcggctg tcgttaacac gaggcggcaa cgtgcgctac
     1441 cagctcaaga cgccgtaccg ggacggcacc acgcacgtca ttttcgaacc attggatttc
     1501 attgcaaggc tggccgccct ggtaccgaag cccagagtca acctaacccg cttccacggg
     1561 gtgttcgcac ccaacagtcg gcaccgggcg ttggtcacgc cggcaaaacg gggcaggggc
     1621 aacaaggtca gggtggctga tgaaccggca acaccagcac aacggcgagc gtcgatgaca
     1681 tgggcgcaac ggctcaagcg tgttttcaat atcgacatcg agacctgcag cggctgcggc
     1741 ggcgccatga aagtcatcgc ctgcattgaa gaccctatag tgatcaagca gatccttgat
     1801 cacctgaagc acaaagccga aaccagcggg accagggcgt tacccgaaag ccgggcgcca
     1861 ccggctgagc tgctcctggg tctgtttgac tgacgagcct gaaggccaac gataccaatc
     1921 aaaatgctgc gttcacagcg ccgcggcagg gatccgccgt gctggttgtc ggaaaaggag
     1981 ccgctagtgg gaaagaggag ggtaaatttt cagcgttgct ggctccccgt cagccggatt
     2041 gggttgcatc gcaggggtgt cgaaagagtc aactgcggtc caaagctgtt ggacttgggt
     2101 gaaaagggcg tttattcttc ctatacgttg tcggcagcgg gccaaaaagg aatacgtcca
     2161 tgcccatcga ggtgaaaccg gctgtgagcg cgggttcaag catatagccc gacaggcgcg
     2221 tatccttgcc gatcacgaca cgatggcggt ggtcaccgcg acgaaagaca cggccagccg
     2281 ccatgccgac gcgcaaggcg gtttccgccg tcatcgcgcc ttcgttggct ttgccacgaa
     2341 taccgtctgt gccgaaatat ttgcgcacca taaggtcgat tatcctgtcg tcgggtcgcc
     2401 ctcaaagggg acatgcctgc tgaaccgcga atatagagaa atatcccgaa tgtgcagtta
     2461 acgaattctt gcggtttctt tcagcgccgc caataccgcc agcccgtcgc gcaaggggcg
     2521 cggctcgtgt gtgcggatga agtcagctcc acctgcggcg gcggcaagct ctgcagcgag
     2581 tgtcgcggcc ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa aggatttgcg
     2641 cgaaacagac agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga accgcgccag
     2701 caccgagagc gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg gatcaaggac
     2761 aaggcggttg cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt caaagaacgc
     2821 cgcaatgtga tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct gcccgtcttg
     2881 caccgaatgc ataacgacga gtttggcaga tgatttcgcc aattgcggat agaacgcagc
     2941 gtctggaaaa ccgcgaatat cattgagata ggccacacca cgcgacaagg cataggcttg
     3001 cgtcgcgggt tgataactgt cgagcgagac gggaatgcca tctgccttga gcgcgtccag
     3061 caccggcgcg atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt cgggattgct
     3121 ggatgccgga ccgaggtcga tcacatctgc cccctcggcc atcagcttac gcgcctgcgc
     3181 aatggctgcg tctggcgcca gataccggcc tccatcggag aaactgtccg aggttatgtt
     3241 gacgatgccg aaaatgatga gcgatttatt catgggggct tctataataa taataatcga
     3301 gcatgagtct catacggatg ctcgggtcga aagggaatcc ccaggcgagt aacctgtttg
     3361 cggtgatcca ttagctgcag gagcagaata gcatacatct ggaagcaaag ccaggaaagc
     3421 ggcctatgga gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt
     3481 ttctgagcat ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat
     3541 aaaagataaa aatgtcttgt ttacaataga gtgggtatcg gagacgaaga aggacaaggt
     3601 acatgctgac cgataccaag ctgcgcaatc tcaagcccag ggacaaactc tacaaagtga
     3661 atgaccggga aggtctctat gtgggggtcg cctcagaaaa cggaatctat ggtcactccc
     3721 gtttttgcaa caccgatttt gacgacaagt tggcttgctt gaatctatcc ggcgtctgaa
     3781 tgggatttta ttcccgcgcc tcgatgagtt ccgcgcctga tgaacctcca gaaaatatac
     3841 ggcttcaatg agcctttccg ttttacaggt tcctcaacag gccggtgggc cgttagtatc
     3901 atcaatatca gtattcgcaa aaccagatga atgattgttt aaactggtgt atttctgcct
     3961 ttatgcttcg taagtttgct gtcgcgccgt cagtgcccag gctattctgg ccagcttgtt
     4021 tgccagagca caggtgacga caaagttgct tttccgacac aacaactccc tgacccagtc
     4081 ggccaacttg ccagactggt gttccagttt ttgtatgaat accctggcac actgaaccaa
     4141 caaagttcgg atctttttgt tgccccgctt gctaatccct aacaatgtcg tccgacctcc
     4201 cgtgctgtac tgtcggggta ccagccctgt tgccgccgca aagtcacggc tgctggcgta
     4261 ctgcttcccg tcgccaatct cagttgaaat agtactggca gtcagcgttc caacgcaggg
     4321 aatactcagc aagcgctgtc caacctcatc ttcgtccaac tttcgtttca actgagattc
     4381 cagatcttta atctgctcaa caagatagtg ataatgctgt tgtaatttca gcagtaactg
     4441 gctgagataa agaggcaaac tactgtcctc aagaagggta ctcagtcgac taataacggc
     4501 agcacctcgc ggaacgctga taccaaattc cagcagaaaa gcatgcatct gattagttgt
     4561 tttcacctta tcctgaacca gggattcacg gacacgatgc agagctcgca ttgcctgctg
     4621 agattcggtt ctgggctgca cgaaacgcat agatggacgt gatgctgctt cacagatagc
     4681 ttcagcatca acgaagtcat ttttgttgct tttaacggca ctgttgcaaa gttagcgatg
     4741 aggcagcctt ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca
     4801 tttcgcccag gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca
     4861 tcacctcaat acctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg
     4921 cgccgattat ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa
     4981 tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc
     5041 gaccataggc gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt
     5101 tgttgaggat tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat
     5161 aaaaatcgac agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat
     5221 tgaccttcac gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt
     5281 accagcgcag ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg
     5341 agtgatcgac attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc
     5401 cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt
     5461 tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac
     5521 tatttgcaac agtgccgata gtttggctgt gagcaattat gtgcttagtg catctaacgc
     5581 atagttgagc ggcgggcgca gcccgtccgc ttgaacgccg agttaggcat cagatgccct
     5641 cggcgcgggt cgatgcactt ttcgcacatg ccgctcaacg caagattctc tcaatcgttg
     5701 ctttggcata tcgaacgaac gcggccgtct cttcgacgcg cattgctagg tcgtcgtcct
     5761 cgctacccag gtacgccgcg cgtgccttgc agatgagggg ccgatgctcg gcaggcaaac
     5821 gctccgatac ccatgcggca gcaacgtcct taggagcaat gagaccagtt gaagcgctgt
     5881 accaaatgcg agcaagagca agaacgacgt tccgctcgtc acccttccaa tccgactctg
     5941 cattccactg ggcaatagtg tcgaaaagcg ccttggagaa atgctccttc ggcaccggct
     6001 cgaaaaacgt ggctgcggat gggcctagaa gcgcaaggct gtgttgcctc gccttggtca
     6061 gcaaaatcgc aagatcgtga tccagaacgg caggctcgaa cgttccggaa aggatgtcgt
     6121 ggcggagcca ctcaccgaac tgaagctcac gccgcgccgg atagcgccaa ggcactactt
     6181 cgcttcgagc gacaacagtt agctccagcg gtcgccatgt tccgccatcg cctggcggtg
     6241 atgagacttt cagcaaatcg agcattagcg cctgccggag cgaatcgtta ggtgcggcgc
     6301 tgacggtcac gagcaagtct atgtcgctgt ccggcttcag ccctccatcg atcgcagatc
     6361 cgaacaggtg gattgtgtcc agtgtcgcag ccagatggcg ctcgatcacc gcgcgagcgt
     6421 gggacagctg cttgaaaact tgtgcaggga aaaattcacc catgatgcct aacgttaagt
     6481 tcagcggcag cttttaagtt gcggctttgt ggaatacttt tgcgcagcaa aaccacaaag
     6541 acgcgactta aaagctgtcc aaggagcgaa gcgactggtg ctgcaacgca ttgttagcct
     6601 tttttccaaa tctggtatgt ataatttata ttagacataa aaaactgttc aaaaaccaaa
     6661 ttgaaattct caggcattat agggaatttg atatcacctt cgacttcaac gtgaacagta
     6721 gacaaatgaa ttatatctgc tttttcaata aggctatt
//
LOCUS       DAFAVF010000196         6743 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFAVF010000196 DAFAVF010000000
VERSION     DAFAVF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.198"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            95..170
                     /locus_tag="LGK42_004028"
     tRNA            95..170
                     /locus_tag="LGK42_004028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Asn,seq:gtt)
     gene            complement(208..1158)
                     /gene="cbl"
                     /locus_tag="LGK42_004029"
     CDS             complement(208..1158)
                     /gene="cbl"
                     /locus_tag="LGK42_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBK2660206.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1260..2177)
                     /gene="nac"
                     /locus_tag="LGK42_004030"
     CDS             complement(1260..2177)
                     /gene="nac"
                     /locus_tag="LGK42_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBK2660207.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            2503..2578
                     /locus_tag="LGK42_004031"
     tRNA            2503..2578
                     /locus_tag="LGK42_004031"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2536..2538,aa:Asn,seq:gtt)
     gene            complement(2634..3569)
                     /gene="ldtA"
                     /locus_tag="LGK42_004032"
     CDS             complement(2634..3569)
                     /gene="ldtA"
                     /locus_tag="LGK42_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBK2660208.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(3631..4710)
                     /gene="cobT"
                     /locus_tag="LGK42_004033"
     CDS             complement(3631..4710)
                     /gene="cobT"
                     /locus_tag="LGK42_004033"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBK2660209.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(4722..5465)
                     /gene="cobS"
                     /locus_tag="LGK42_004034"
     CDS             complement(4722..5465)
                     /gene="cobS"
                     /locus_tag="LGK42_004034"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBK2660210.1"
                     /translation="MSKLFWAMLSFITRLPVLRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(5462..6007)
                     /gene="cobU"
                     /locus_tag="LGK42_004035"
     CDS             complement(5462..6007)
                     /gene="cobU"
                     /locus_tag="LGK42_004035"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBK2660211.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIYADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         1803 a         1803 c         1485 g         1652 t
ORIGIN      
        1 acaaactgtt gatagaaacg gcaaacagtt ggggaattta aaaatcgggt ttgacaaaag
       61 atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg
      121 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa
      181 aagcccgctt ttatagcggg ccttttgcta tatctgataa tcaatttcct cttcactgtt
      241 ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa
      301 gcgccagaca tagttacgct gaagttgtcc tcgcttgagt cccaaccaga cagtattagc
      361 atcgaaaaga tgccgcgtat ccaggcggat taaattcttt tcctcttgtt caccgctgga
      421 ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga
      481 atcctgcgca cttaatacaa tatctgccag caaacctttg cgggcaaatg cgtcatcaat
      541 acgtgagcgc cccgtaatcc cctgtcggta agtgattaac ggccacttcg ctattgattc
      601 cagcgtcaat ggtgtaattt gcgtcaaggg atgatcgtgt ggaacaagca aactatggtg
      661 ccaacgaaac cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat
      721 accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg taccctggat
      781 tagctccagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct caggcaagct
      841 ataacgtgcc tgagtatgcg ttgttgcaat agtgagaacg ccagacgtat cgttggtaaa
      901 caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag
      961 taatgctttg cccggttcag tcatgcccag cagtcgctta cctcgtcgaa caaatatttc
     1021 gatgccaagt tcatcctcca gttcccgaat atgacggctg acgccggact gtgaggtaaa
     1081 aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt
     1141 tagttgttgg aaattcacgg taaactccgg gcagttcaga ttttccgtta ttgttaaagt
     1201 ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct
     1261 tagctcacca attgccactg ccttttttcc atcactggag aactcatcac tgacattaac
     1321 aactctttca ctgcctgtgc ctgtggtgat aagttcgctc tggcgggtaa atttaatgac
     1381 aaagagagac tcatggaagg tgtggtaatg cgtgacatcc acccgtttac tgcgccacat
     1441 aacgaacgcg cggccgattc gggtaatact gcaacgccca tgccgctggc aatcgctgcg
     1501 gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagtcg ccgtagggaa
     1561 aaagcctcat caacacgaag tctaacagca ctgtaatcac tgggaagaaa gaggttcatt
     1621 tgcgcaatag cattaacatc aacgctttgc cccggacaat cttgagtccc taccaggaaa
     1681 agatcttctt tcagtaaagc ctgactggat acaccagcca caggggaatg ctcataaatc
     1741 accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc actattttca
     1801 tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg
     1861 atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga
     1921 ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgcgcatgg
     1981 gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagt
     2041 tgttgattta actcaccttc cagtgtggca acctgctggc tgagcgctgg ttgtgcgata
     2101 tgcaatactt cagcagcctg agtcaggcta ccaatatcta caatttttac gaagtatttc
     2161 aggcgtctga agttcatgtt gcctccggtt tttgagaatc ggcccaagtg ccgccattac
     2221 ttacaaccag actgcaagat gcttgccagt tttattttgg cgttgatgta caagctaacc
     2281 aactgtcaaa taagagatta tgatagattc gtcattggct cttttcacca gcagtcgcgt
     2341 tcccctgccc tataaaagga gggtatgcac cacgatggtt cattaccaat aagattgaaa
     2401 tctcatcact ttgttgaaat tgacagcaaa caaacaaaaa aatgcatttc accctttgac
     2461 atcaccatgc actgccatta atatgcgccc cgttcacacg attcctctgt agttcagtcg
     2521 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     2581 ttcaaaaaag cctgctttct agcaggcttt ttgctttcta attaccaacg ctcttaaaac
     2641 atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgacgttg caacgcagca
     2701 tttgcgcgat tcacatcaac ttcttgcccg ttgataaacg cccgcaaaga tggggttacc
     2761 ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacag cggctcatga
     2821 acttccagcc agttcgagcc atctggttca gtggtgtatt ttactggctg gtcgataatt
     2881 tgcacacgcg ttccaacagg aacattatca aacagatatt tgatatcgtc attgcgcaga
     2941 cgaatacagc cctgacttac ccgaagtcca ataccaaaat tggcattggt accgtggatg
     3001 gcatacaacc tgccaatata aatcgcgtac agccccatgg gattatcggg ccccgcagga
     3061 acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc
     3121 gtccaggttg gagcttcttg tttacgttca acggtagtca cccagttacg cggggtttct
     3181 cgcccagcct ggccgatacc aataggaaag acttccacag tattactgtc tggcgggtag
     3241 taataaagac gcatctcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa
     3301 atcagttgct gcggaatggt gagttgcgag ccagacttcg gcaagaaaac atcagcgccc
     3361 ggattcgctt ccagcatgtt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc
     3421 ggctgggtat tgtgatcagg aacagttaca gtaagcgact gccccaccaa acggctaccc
     3481 tctggaggta atggataagt taccgccagg ctagtatggc tggcaaaaag cagagcaaat
     3541 gagcaaagaa tatttacacg acgcatcatg tccctttcct atgtcgcgaa agctatccgt
     3601 taagtatagc ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc
     3661 cggtagaaca atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc
     3721 ttcgatgatg ggcatcgcca gagctgcgcc actcccctca cctaaacgca tctccatatt
     3781 gagataaggc tccagcccca aatgcgacag cgctatacgc gccccttttt ctgccgacag
     3841 gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcagc
     3901 agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat
     3961 cacgccagcc attccgacca aatcaaatcc ccccactttt gccagaacat caacgccatc
     4021 ctgcggattt ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag
     4081 tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc ggccagtgat
     4141 tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa
     4201 cagcgtgaca ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt
     4261 ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac
     4321 acgcatgttg ataagcccgg ggataggctc agcactatca ataccaacat caactacgtg
     4381 gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt
     4441 ttcagcctga atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc
     4501 gtgatcggca cacataacca gtaccgcttt tttgcccaca tgcggtatgc cattcaaccc
     4561 cggcattccc gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt
     4621 gagtaacccg tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga tagcagggat
     4681 cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg
     4741 aagaccagtt caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc
     4801 gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc
     4861 acaccatgca tacccggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg
     4921 caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc ttcccgggca
     4981 taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc
     5041 agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc
     5101 agtacaacaa aaatcaatgc cagaccgcca tgggtgccta aacggctatc acgcatgatt
     5161 tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg
     5221 tctaggtgga atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt
     5281 ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat
     5341 aatccaatta aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg
     5401 gaccagcgac gcagtacagg cagacgcgta ataaatgaga gcatcgccca aaataattta
     5461 ctcatttgat ttttactcca atacccgaaa ccaccagcca tacttcattt gccgcagcgg
     5521 ccagctgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg
     5581 gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg caacgttggc
     5641 aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc
     5701 attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa
     5761 cgcattccag caacacagct tcatgcgggt taatgtctgc ataaattaat tcatcaagat
     5821 gttgccaacg ctccaccgtg cgccagtgcg ccggacggcc ttgccgatga tgttctatcc
     5881 gtgcagccat ctcgtcatca aggatttgcg aggtggcgat atacagaacc tgtgaagagt
     5941 ccccaataag cgcctctgcg tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa
     6001 tcatcatacc gactcctgat tcaattgtat gatggtattc attgaacaga acaatggatc
     6061 ccccaccatt gctgaccgca aaacatttac acgatcaata ctgcgcgtaa tataattaaa
     6121 aatccagcat tctcaatcca ttacgaaaga agatggcatt gtggaatagc cagacttcct
     6181 cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctgtggattg acacatttca
     6241 ttcagataat gaattaatgc tactgccgga ccaaccagca aacgggcatt gtgttctgaa
     6301 atccaggcta ttgattcaac gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca
     6361 acgcaataga attatccgga atggtggcga ctatgcacta gaacaccaca atttcgctct
     6421 ctcggtaagg cactgtcaca gcattcaaca gaatgtgact tgccagattt attagcgcca
     6481 ccagatgttt aaccgggtaa ccacgaccca gtcggggata tattccatct tctcgctgac
     6541 atcatcaaga ataaaaaggt ttatcacact cagagcaacg ttcttcagca ggcgggataa
     6601 ttcttcaaca tttaaaagat gtgttattaa aggcatacaa actggacgta ttgtattttc
     6661 ttttggtggt tgacctaagg tagcagttta tcctgatgcg ctgagatttc tgtaatatct
     6721 acgtcaacat tctcctcgaa tag
//
LOCUS       DAFAVF010000197         6722 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFAVF010000197 DAFAVF010000000
VERSION     DAFAVF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.199"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(130..615)
                     /gene="sixA"
                     /locus_tag="LGK42_004036"
     CDS             complement(130..615)
                     /gene="sixA"
                     /locus_tag="LGK42_004036"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBK2660212.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(818..2962)
                     /gene="fadJ"
                     /locus_tag="LGK42_004037"
     CDS             complement(818..2962)
                     /gene="fadJ"
                     /locus_tag="LGK42_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBK2660213.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2962..4272)
                     /gene="fadI"
                     /locus_tag="LGK42_004038"
     CDS             complement(2962..4272)
                     /gene="fadI"
                     /locus_tag="LGK42_004038"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBK2660214.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELHRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4452..4736)
                     /locus_tag="LGK42_004039"
     CDS             complement(4452..4736)
                     /locus_tag="LGK42_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBK2660215.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5108..6448
                     /gene="fadL"
                     /locus_tag="LGK42_004040"
     CDS             5108..6448
                     /gene="fadL"
                     /locus_tag="LGK42_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBK2660216.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1661 a         1822 c         1607 g         1632 t
ORIGIN      
        1 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
       61 cagatatccg ctgcctgatg agcaattatg cccaatgaat ctacctcatt gggcataatt
      121 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
      181 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
      241 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
      301 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
      361 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
      421 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
      481 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
      541 agtggtcaga ggacgaacgg aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat
      601 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
      661 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
      721 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
      781 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
      841 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
      901 gggaaccata ctgcgtggca agtcgttgca ttattgcaac cacttcgccc gcgccgagag
      961 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
     1021 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
     1081 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcgc ccctgccctt
     1141 gtgcgccaat gagcgggtaa atggcgggat cgacctgttt tttgctttta cgccctttct
     1201 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
     1261 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
     1321 taattttagt cccggtgtcg attcctacct catccaaaag ttggattgga cctaccggaa
     1381 aaccaaattt cactaacgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
     1441 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
     1501 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
     1561 gcgctgatgt acccgcatga ggaataatct ccaccagcgg cattttttcc accggactga
     1621 agaaatgcag gccgataact tgctcaggtc gcgtggcgtg agcggcgata tcaccaatcg
     1681 gtaaagatga cgtattcgaa gcaaagatgg tatgagcggc gctattttgc tcaacttccg
     1741 ccaccatctg ttgtttcaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
     1801 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
     1861 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
     1921 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
     1981 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
     2041 atggcgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
     2101 tactacgcag cgcctgcgat tgtggcgtca tcgccagttc gccaaacgcc cgagcttcgg
     2161 cgtcataacc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
     2221 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
     2281 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
     2341 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
     2401 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
     2461 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
     2521 cgggtaacaa tccaagttgt acttcaggca aaccgagcac cgttttagga tcgtcagtac
     2581 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
     2641 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
     2701 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc gatcatgttg atgtctgcgc
     2761 cagcaatgaa gttgtccggt ttagcggaga caaacaccac gcctcgcaac tctttgtttt
     2821 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ctccgccttc agggtattca
     2881 ttttctcacc cggtacgtcg atggtgataa cggcaatgtt gtccagacga acattaaggg
     2941 taaacgctga tgtcatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
     3001 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggtgaagtt catgcaatgt
     3061 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgc ccgtaagcaa tcgaaccgcc
     3121 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
     3181 acgagcaaaa cgttcgctgc ccagcaactg aatattcgcc agtgtctgag cggcaaaggc
     3241 ttcgtgcata tcgatcaatg tcagatcgct catcgtcaaa ccggcacgct ccagcgccag
     3301 cggtgttgac caggccggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
     3361 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgctcggg attcagtcat
     3421 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
     3481 gtgtttacga tcaaacgccg gacgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
     3541 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
     3601 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
     3661 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
     3721 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
     3781 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
     3841 cagcacgcgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
     3901 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
     3961 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
     4021 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
     4081 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
     4141 caccatcttc cctaaatcaa ccgcgggaat gccatgaaaa gccgtcccct ggcgggcaaa
     4201 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagcggtaa
     4261 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
     4321 tcttaaccat ttttttacat ttagccaagc ggagaaaaaa gaaagtggga gctatgacac
     4381 agtgaagaca ggagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgaacgta
     4441 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
     4501 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
     4561 cacggttcgg attccacgct acgggctttt tcagtcagcg ccgccagcgt ttgttctgct
     4621 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
     4681 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
     4741 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
     4801 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
     4861 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
     4921 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
     4981 cagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
     5041 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
     5101 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
     5161 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
     5221 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
     5281 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
     5341 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
     5401 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
     5461 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
     5521 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
     5581 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccgtct acgctcgcgc
     5641 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
     5701 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
     5761 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
     5821 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
     5881 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
     5941 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
     6001 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
     6061 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
     6121 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
     6181 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
     6241 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
     6301 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
     6361 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
     6421 cggtactaac tttaactacg cgttctgata acacgttcgc ctggataaag tcacctgcat
     6481 agcaggtgac tttaactccc ccacttcacc gaaagtagtg tcctcattgc ttacctcctg
     6541 agttttgcaa acagcctgat ggcagcttgc cctttcaaat caataagcgg tatccacaga
     6601 caaatctgaa gggaaaggca tattttcagg cgttctgctc gtccttctca aagagtttac
     6661 ttttctgaat ttccaggata ctcacccccc cctggctatt gtgcgctcat acactcaaat
     6721 ta
//
LOCUS       DAFAVF010000198         6652 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFAVF010000198 DAFAVF010000000
VERSION     DAFAVF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.200"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(127..2757)
                     /gene="alaS"
                     /locus_tag="LGK42_004041"
     CDS             complement(127..2757)
                     /gene="alaS"
                     /locus_tag="LGK42_004041"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK2660217.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(2885..3385)
                     /gene="recX"
                     /locus_tag="LGK42_004042"
     CDS             complement(2885..3385)
                     /gene="recX"
                     /locus_tag="LGK42_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK2660218.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(3628..4689)
                     /gene="recA"
                     /locus_tag="LGK42_004043"
     CDS             complement(3628..4689)
                     /gene="recA"
                     /locus_tag="LGK42_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK2660219.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(4769..5266)
                     /gene="pncC"
                     /locus_tag="LGK42_004044"
     CDS             complement(4769..5266)
                     /gene="pncC"
                     /locus_tag="LGK42_004044"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBK2660220.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(5411..6496)
                     /gene="mltB"
                     /locus_tag="LGK42_004045"
     CDS             complement(5411..6496)
                     /gene="mltB"
                     /locus_tag="LGK42_004045"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBK2660221.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1517 a         1905 c         1606 g         1624 t
ORIGIN      
        1 catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt
       61 tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg
      121 cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa
      181 ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc
      241 cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt
      301 agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt
      361 cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc
      421 gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt
      481 tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg
      541 cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag
      601 atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc
      661 ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa
      721 cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc
      781 gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc
      841 tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat tgcgacgaat
      901 ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg
      961 tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt
     1021 ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat
     1081 acgggcgcga cgagcctcat caacatcagc ctgcacagcg tcgcccactt tcagagaacc
     1141 cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc
     1201 gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc
     1261 atagaatggt gtttgatcca gcacgaccac agcttcctgg cctgcattga tggcatcaac
     1321 cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc
     1381 tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct
     1441 ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac
     1501 tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc
     1561 gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc
     1621 atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag
     1681 cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc
     1741 gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg
     1801 acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat
     1861 tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga
     1921 tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa
     1981 caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc
     2041 cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt
     2101 gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact
     2161 tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc
     2221 gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt
     2281 atcgccgatg cgaataatac gttcgcgcgg gatccctact tctttttccc agatttcgta
     2341 ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca
     2401 tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc
     2461 gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt
     2521 ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga
     2581 ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt
     2641 aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc
     2701 cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat
     2761 tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg
     2821 ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat
     2881 gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat
     2941 agagcaaaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat
     3001 attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg
     3061 ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctggcga atacgcgcag
     3121 gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga
     3181 gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa
     3241 tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct
     3301 cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg
     3361 ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc
     3421 gcagatgcga cccaggtttt gctgataaac agatttaata gtcaatgagt gtgcagaatg
     3481 ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg
     3541 gcctgttccc ctcaccctaa ccctctcccc aaaggagcga ggggaccgtc cgtgcacaca
     3601 ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctacgccttc
     3661 gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac
     3721 tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc
     3781 tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag
     3841 cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag
     3901 gatctgaaat tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg
     3961 ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag
     4021 acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa
     4081 catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt
     4141 caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg
     4201 agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac
     4261 gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc
     4321 gccggtgtcc ggctgggagc acagcaggtt atcaatatcg acgcccagtt tacgtgcgta
     4381 gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc
     4441 tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc
     4501 gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc
     4561 ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt
     4621 accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc
     4681 gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga
     4741 agcaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg
     4801 tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcaccg ctgaagcatt
     4861 cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag
     4921 gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag
     4981 cacgagccgc tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc
     5041 catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt
     5101 aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg
     5161 ctacccaacc accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg
     5221 cctgcccaac ctgttcactt aactgcatca gttcactgtc agtcatcaca acctctgtca
     5281 gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca
     5341 attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc
     5401 ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca
     5461 ttgcgtaatg ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc
     5521 agtactggta accggtaccg acatccagac gcagcaggct ggcttgttga tggttgccca
     5581 gcggctgctg tggcgttaaa cctgccgtgg caagctgcga aatgctgtac ctggttttga
     5641 agccatttgg caagcctgga gcctgaccgt ttgccattac cgcgacctga tcgcctttca
     5701 cccagccgtg cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca
     5761 ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa
     5821 actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct
     5881 cgtcgcgcgc catcaacagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg
     5941 ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc
     6001 cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct
     6061 gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt
     6121 tgtccggcgt aataaatttt ttgcgataac ggagccatgc gccgttcgga cctgatggcg
     6181 gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct
     6241 tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt
     6301 tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat
     6361 tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag
     6421 taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat
     6481 aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa
     6541 gcatcacgcc ccacacaagg aagcggtagt cactgcccga tacggacttt acataactca
     6601 actcattccc ctcgctatcc ttttactcaa actttcaaat taaaatattt at
//
LOCUS       DAFAVF010000199         6638 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFAVF010000199 DAFAVF010000000
VERSION     DAFAVF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.201"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            89..1414
                     /gene="dptG"
                     /locus_tag="LGK42_004046"
     CDS             89..1414
                     /gene="dptG"
                     /locus_tag="LGK42_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBK2660222.1"
                     /translation="MYPIATNLKVSNNQLDSYLPIRNKNNNIDWQIVTGLVLSYAVKY
                     KIDTYSLEQFREDCKTHLQILIDEPAFLSVLERMYFSSQDIFRVSPLFLLFHAQFDGE
                     KISAGSTADKRLGTLFANLMRDFSLNNPIQDKLNFIEKEMLNKLNKKLIRLGEGPFAK
                     EQPYLPYLVTCFQSDLAFLAEHPQYLLQELTNTLRLYAFSWCAQLALNLDNWQDGEPQ
                     SKSLFFILDTEKASSERDKIKLFGYKWFARQSEKLFPVLSALEVLQVKGEEKRPLWQV
                     YQDCLGYSDTSNRVLNELNNYIQKFISKEERDLPERDRATNLEDAFKQLLSVAVEQFQ
                     GKKTERAAVNRKYINELESQICTDFIQVRGRAGKVLVLNQDRLLLLTNLTVGKNKKLR
                     LHELLRGFEQRGFYLDNQSTQMLVAFYERMGNVERMSDSGDAVYVRKTV"
     gene            1395..6458
                     /gene="dptH"
                     /locus_tag="LGK42_004047"
     CDS             1395..6458
                     /gene="dptH"
                     /locus_tag="LGK42_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HBK2660223.1"
                     /translation="MSVKQYETYLAETFIEWVSSIIQPGERYQFKSPDPDNALKLWKA
                     FDSLANGNKLEIAPEQFLTYVQCNNIQLIPVLHGTTTPAFTENYISHLRDQVSGRNGI
                     FAQTALLIIHNSMLDTLLNSTKDVAAPDAIWHPETFRHQLEKLITTDSARSQVSRCLL
                     GDQLTTILDEGATVFGFSSLYRLLDDGNLDFSELKLFNDNNLLNFSDKQLRARLNENQ
                     KLYRQIEDSVERYSGQLENVLTEFSAKFIQEHFVDNDDWRELDFSVYLNEKAQNSEQK
                     LVLENITAENGEVWQRAKSTSKAGKRDISVLVQVLPGQSQVELEFIFQGNDLQDNQVK
                     IAHHRPLQKERFWRTSRLGGRNSKILASVPFDGRPCFFSLEIINRNNSAEEYKFRLLL
                     VEQGQFWLNEIQHCYRIEPGKGQLTLQLEDNELRIAEFGDKTCLLDEESDDVDCRHYA
                     LVNFETLANQSELIKFTLISGDSRLLFNIEGPGAEEGLTLPLLFDQGRFNKLFKEEGN
                     ATWNKLKGRVILDNIEHNVVGVRQQLLMQEYALIERNLLSTGSNGSEFALDELLASYP
                     DLHNAYHQLFVYYQRRNTLPSLVSWSAEYRVLVSHMIATFEQALQQISLSRALTIQEK
                     RLLHLGICRGDDYERLSPLHPLVMAYHLQLAETIIAEPGYPTSASFASLPEITLDRLV
                     VSGLMPFVYHSEHEYAQLQPMVENRFWIDIVPQRQMSHEYVKRLVKDKLNEFTDAYSR
                     LFQSAGNNALVINAINMGEARELFLGLVDYFKQEKDNAISIHVNCYDERLLPNAFDRF
                     AESGSYEQLKNDLGLNSGAWRSEADMLIDLLRSRLTFSKFILPPESDQLAYAHLAFFT
                     NTAPVDCRQIRIEDAASGVLCHGLIAGEGAETQGDAYFTAFGLRNVDTESSCALRLAR
                     LLGCLWQPARQSNSQYHGQGISLAVSGNFKQLLNRSYDSSLWTTIIDPKVTLDFFTSQ
                     KDVVLIHYSDQYTSCAGYDAVTVTKQVELFLRLLQTGQSTVDSQQLLAEFNAFNGEWL
                     LKMLRSNEKERKEKHGIIGAYKFVQSMLLQSDICWIPLSVAEMIRVSGNVGLRMKESD
                     LSRHLQGYQKGAISDDVLFVGFKDNNLYLLPLEVKTGARPDYNHAGQQARELKRYLQQ
                     DILGPKTLASQLYRALFIRQVLMQVEKLRLYGVLDNDKLSPLLERREWWLTGDYQLGE
                     LADYAEGFVVAHVDSGTCFDLSYKETAENILQIEIPYSLLPSLIAAQGDQPPLAERYR
                     VPEKYLLKPECDVNSSHPVQVATTTEPPPISALELPQPEASALTPSNVRNTPLQILFG
                     HDAVRQNPLYWEPTNTAKFMNTNTGIIGTMGTGKTQFTKSLVTQLMRNQSYNVDGKPI
                     GLLIFDYKSDYVDDAFLEATGAKRYQLSLLPYNPLSLFGDMPMLPRHTAMAFAETMGK
                     AYNLGVKQRMKLVTLIMECYDLAGIVPHDRSTWNRVAPTIEDVWQQYLAQEKVDEDSL
                     YAALYNLAGFQIFETDPEKMTSLYDLVDGVTVIELAGYPSEIQNLVVALTLDLFYAQM
                     QKRGKPTVRGDYRQLTKMILVDEADNFMRQDFASLRKILKEGREYGVGAILSTQEITH
                     FKTGENNYASYILTWVIHRVSEIRNSDIKAVFNIDDKSEQESLMGQIRQLEKHFSLYI
                     DGNKKVRKMRDKAFWELMK"
BASE COUNT         1906 a         1437 c         1530 g         1765 t
ORIGIN      
        1 atcaagtttt atgatggcct aaagcgggtc tatagtgcaa gagcggacga tgacatgatt
       61 actatcagcg gcatggaggg ataacatcat gtatcccatt gcaacaaatt taaaagttag
      121 taataaccag ctggatagct accttccaat acgtaataag aataacaata ttgactggca
      181 aatcgtcaca ggtttagtac tgagctatgc cgtgaagtat aaaatcgata catacagtct
      241 cgagcagttt cgcgaggatt gtaaaactca tctgcaaata ttgatcgatg aacctgcgtt
      301 cttgtccgtg ctggaacgga tgtatttttc cagtcaggat atttttcgtg tctccccgct
      361 gttcttgctg ttccacgctc agtttgatgg tgaaaagata agtgcaggct cgactgccga
      421 caaacgcctt ggaacactgt ttgccaattt gatgagagat tttagcctta ataacccgat
      481 acaagataag ttgaatttta ttgaaaaaga gatgctgaat aagctcaata aaaaattaat
      541 ccgactgggt gaggggccat ttgcaaaaga acaaccttat ttaccctatc tggtaacgtg
      601 ttttcagtct gatttggcgt ttctggcaga gcacccacaa tatcttttgc aggagctgac
      661 caatactcta cgtttgtatg cctttagttg gtgcgcacaa ctggcgctca atctggacaa
      721 ctggcaagac ggcgagccgc agagtaaatc attgttcttt atcctcgata ctgaaaaagc
      781 tagctccgaa cgagataaaa taaagctctt cggctataag tggtttgccc gccaaagtga
      841 aaaattattc cccgtactct ctgcattaga ggtattacaa gtaaaaggtg aggaaaagcg
      901 ccctctttgg caggtatatc aagattgctt aggttattca gatacatcga atcgagtact
      961 aaatgaactc aataattata tacagaagtt cattagtaag gaggagcgag accttcctga
     1021 acgcgatcgt gctacaaacc tggaagatgc ctttaaacag ctgttatctg ttgccgttga
     1081 acagtttcag ggtaagaaaa ccgagcgagc agcggtaaat cgcaaataca tcaacgagct
     1141 ggaaagccag atttgcactg acttcattca ggttcgtggc cgagcgggga aagtgctggt
     1201 actgaaccag gatcgactac tgctactaac taaccttacc gtgggtaaaa acaaaaaact
     1261 ccgtctgcat gagctgctac gtggttttga gcaacgcgga ttttatctgg ataaccagtc
     1321 tacacaaatg ctggttgcgt tttatgaacg catgggaaat gtggagagaa tgagtgacag
     1381 tggagacgcc gtatatgtcc gtaaaacagt atgaaaccta tctggcagaa accttcattg
     1441 aatgggttag cagcatcatc cagcctggtg agcgctacca gtttaaatct cccgatccgg
     1501 ataacgcctt aaagctctgg aaggctttcg actctttagc aaatggaaat aaactagaaa
     1561 ttgcaccgga gcaatttctt acctacgtcc aatgtaataa tatccagctt attccggtac
     1621 tgcacggcac cactacccct gcattcacag aaaactatat ttctcattta cgcgatcagg
     1681 tttctggccg caatggcatt tttgcccaga cagcgttgtt gattatccac aacagcatgc
     1741 tggatacctt actcaatagt accaaagatg tcgctgcacc ggatgctatc tggcatccag
     1801 aaaccttccg tcaccagctc gaaaagttaa tcacaacgga cagcgcccgt tctcaagtat
     1861 cccgctgttt gctgggcgat cagttgacaa caatattgga tgaaggcgct acggtttttg
     1921 gattttcatc gttataccgt ctgctggacg acggcaatct ggatttctca gagctaaagt
     1981 tattcaacga taataacctg ctgaatttta gtgataagca gttgcgcgcc cggctcaatg
     2041 aaaaccaaaa gttatatcgc cagatagagg acagtgttga acgctacagc gggcaactgg
     2101 agaatgtact aactgaattt agtgcaaaat tcattcagga gcatttcgtt gacaatgacg
     2161 attggcgtga gcttgatttc tccgtctacc tgaatgaaaa agcacaaaac agtgaacaga
     2221 aactggtgtt ggaaaacatt actgctgaaa acggtgaggt ctggcaaaga gccaaaagca
     2281 ccagtaaagc aggtaaacga gatatcagtg tcctggttca ggttctaccg gggcagtcac
     2341 aagttgagct ggagtttatc ttccagggta acgaccttca ggacaatcag gtaaaaattg
     2401 ctcatcatcg tccgttacag aaagaacgat tctggcgtac tagccggctc ggggggagaa
     2461 actctaagat actggcatca gtgccttttg atggtcggcc ctgcttcttt agcctcgaaa
     2521 tcattaaccg caataactca gcagaagagt acaagtttcg cctgctgtta gttgaacaag
     2581 ggcaattctg gcttaatgaa atccagcatt gttatcgtat tgaacctggt aaaggtcaat
     2641 taactctgca actggaagat aatgagttac gtattgcaga gttcggcgac aagacctgtt
     2701 tacttgatga agaaagtgat gatgttgatt gccgccatta tgcactcgtt aattttgaaa
     2761 cgctggcgaa tcaaagtgag ctgatcaaat ttaccttgat ttcaggtgac tcccgtttac
     2821 tgtttaacat cgaaggtcca ggagcagagg aaggattaac actgcctctg ctttttgatc
     2881 agggtcgttt caataaactc tttaaagaag aagggaatgc aacctggaac aagttgaaag
     2941 gtcgcgtcat cctggacaat atcgaacata atgtggttgg tgttcgccag cagttgctta
     3001 tgcaagagta cgccctgatc gagcgtaatc tgctcagcac tggcagtaat ggtagtgagt
     3061 ttgcactgga tgagcttctg gcgagctatc ccgacctcca taatgcgtac caccaattgt
     3121 tcgtttacta tcaacgtcga aataccctgc cgagtctggt gtcatggtca gcagaatatc
     3181 gtgtattagt cagtcacatg atcgctactt ttgaacaggc attacagcaa atcagtctaa
     3241 gcagagcatt aaccatccag gaaaaacgtc tgttacatct ggggatttgc cgcggcgacg
     3301 attatgaacg tctctcgcct ctgcacccat tggtaatggc ttatcattta cagctagctg
     3361 aaactattat cgccgagccc ggatacccca catcagcttc cttcgcttca ttaccagaga
     3421 ttactctaga tcgtctggtc gtctctggcc taatgccatt tgtgtatcat agcgaacatg
     3481 aatacgcaca gttacaaccg atggtagaaa accgcttctg gatagatatt gttccccagc
     3541 gccagatgag ccacgaatat gttaagcgtc tggtgaaaga caagctaaac gaatttacag
     3601 atgcctattc tcggctgttc cagagtgcag gaaataatgc cctggttatt aatgctatta
     3661 atatgggaga agccagagag ctattcctgg gactggtaga ttattttaag caggaaaaag
     3721 ataacgcaat ttcgatacac gtaaactgct atgacgaacg tctgctaccc aatgcctttg
     3781 accgctttgc cgaaagcggc agctatgaac aacttaaaaa tgatcttggt ctgaacagcg
     3841 gtgcgtggcg ttctgaagcc gatatgttga tcgatctgct tcgcagtcgc ttaacattca
     3901 gcaaatttat cctgccaccg gaaagcgatc aactggctta cgcccatctc gcatttttca
     3961 ccaacaccgc accggttgat tgccgccaaa ttcgcattga ggatgctgcc agcggggttt
     4021 tatgtcatgg cttgattgca ggcgaagggg ctgaaacaca aggcgatgct tactttaccg
     4081 cgtttggtct gcgtaatgtc gatactgaat cgtcttgcgc cctgcgtctc gcccgcttgt
     4141 taggctgtct gtggcaaccg gcacggcaga gcaacagcca gtatcacggt cagggaatta
     4201 gtctggctgt gagcggtaac tttaagcaac tgctgaatcg ctcttatgat agctctctct
     4261 ggaccacaat tatcgaccct aaggtcacac tcgatttctt tactagtcaa aaagatgtcg
     4321 tactaattca ttactctgac cagtacacca gttgtgctgg ttatgacgcc gtaactgtca
     4381 caaaacaggt tgagttgttc ctgcgtctcc ttcaaacagg acaatccacc gttgatagcc
     4441 agcaactact tgctgaattt aatgccttta acggtgagtg gctgttaaaa atgttgcgtt
     4501 caaatgaaaa ggagcgcaaa gaaaaacacg gcattatcgg tgcatacaaa ttcgtacagt
     4561 ctatgctgct ccagtcagat atttgctgga ttccactgtc agtagctgag atgatccgcg
     4621 tgtccggcaa cgtcgggctg agaatgaaag aaagcgattt atcgcgccat ttacagggtt
     4681 atcagaaagg agcgatctct gacgatgtgc tctttgtcgg tttcaaagat aacaacctgt
     4741 atctgttacc acttgaagta aaaacaggag ctcgccccga ttacaaccat gcggggcagc
     4801 aggcccgcga gctaaaacgc tatttacagc aagatattct tgggccgaaa actctagcat
     4861 cacaactata ccgggcactt tttatccgcc aagtattaat gcaggtagag aagttgcgtc
     4921 tgtatggtgt actggataac gacaagctat cccctctgct ggaacgacgt gaatggtggc
     4981 tcactggcga ctatcagtta ggtgagcttg ctgattatgc agaaggcttt gtggtagctc
     5041 atgtagacag cggaacctgt tttgatctct cttataaaga aacggcagag aatattctac
     5101 agatcgaaat cccctattca ttgcttccat cgttaattgc agcacaaggt gatcaacctc
     5161 cactagctga acgctatcgg gtgccggaaa aatatctgct caaaccagag tgtgatgtca
     5221 attcttcaca cccagtacag gtagcaacaa caactgagcc gccgcccatt tctgcgctgg
     5281 agcttccaca acctgaagca tcggcactaa cccccagtaa tgtccgcaat acaccattac
     5341 agatcttgtt tggtcatgat gctgtgcgtc agaatccatt gtactgggaa ccaaccaaca
     5401 ccgctaagtt catgaatact aataccggca ttattggaac aatgggcacc gggaaaaccc
     5461 agtttactaa atcgctggtt acgcaactga tgcgcaatca gtcctacaac gttgacggta
     5521 aaccgattgg cttgttgatc ttcgactata aatccgacta tgtggatgat gcgtttcttg
     5581 aagctacggg agctaagagg tatcagctat cgttgttacc ttacaatccg ttatcgttat
     5641 ttggtgatat gcccatgtta ccgaggcata cggccatggc attcgctgaa acgatgggca
     5701 aagcgtataa ccttggcgta aaacaacgca tgaagctggt tacgttaata atggagtgct
     5761 atgacttagc aggaatagtg cctcatgatc gatcaacatg gaatcgtgta gcaccgacta
     5821 ttgaagatgt gtggcagcag tacctggcac aagagaaggt cgacgaagat tcattatatg
     5881 ctgcacttta taatctggct ggtttccaga tttttgaaac ggatccagag aagatgacca
     5941 gtttgtacga tctggttgat ggagtcacag ttatcgaact cgctggttat ccatcagaga
     6001 ttcaaaacct ggtagttgca ctgacactgg atctttttta tgcgcagatg caaaagcgtg
     6061 ggaaaccgac ggtgcgaggt gattatcgcc agttaaccaa aatgattctg gttgatgaag
     6121 ccgataactt tatgcgccag gattttgcca gcttgcgtaa aatccttaaa gaaggccgtg
     6181 aatatggtgt aggggcgatt ctatcaacac aagaaattac tcactttaaa acaggcgaga
     6241 ataattatgc ttcttatatt ctcacttggg taattcatcg agtttctgag attaggaatt
     6301 cggacattaa ggcggtattt aacattgacg ataaaagtga gcaggagtct ttaatgggcc
     6361 agatacgaca acttgagaag cattttagcc tgtatataga tggaaataaa aaagttagga
     6421 aaatgcgtga taaagcattc tgggagttaa tgaaatgaaa ataaatacta cagatccaga
     6481 tttgaggact atattttcta ggattcatga aggaagtcta gatctccaac ccgatttcca
     6541 gagggcggaa gtctggcaac ttccgaagaa aaaactttta atcgatacta ttctaagagg
     6601 gtggcaggtt ccacctgtcc atgttatttt aaatgaag
//
LOCUS       DAFAVF010000200         6636 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFAVF010000200 DAFAVF010000000
VERSION     DAFAVF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.202"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            20..1135
                     /locus_tag="LGK42_004048"
     CDS             20..1135
                     /locus_tag="LGK42_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBK2660224.1"
                     /translation="MFTFLRSSDVPEYITAPVRKGDIENSVLATGRIDAIERVNVGAQ
                     VSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDLRNAEAALNEVKAELQSKQALLKQA
                     ELRFKRQLRMLRENASSHEDFESAEAMLATTRAELLSLNAKLVQAQIEVDKKKLALEY
                     TRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKLARLDVMTIKAQISEADITRISVGQ
                     KARFSIFSEPDKHYSATLRAVELAPESVMKDDSLASNTSASGSGTSNASVYYNALFDV
                     PNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVHRNEGKKQQVLVLAADGRLEPRNVK
                     TGITNSVDIQILEGLNVGENVVLSLPDKKEPEERIML"
     gene            1132..3072
                     /locus_tag="LGK42_004049"
     CDS             1132..3072
                     /locus_tag="LGK42_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HBK2660225.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            3076..4446
                     /locus_tag="LGK42_004050"
     CDS             3076..4446
                     /locus_tag="LGK42_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HBK2660226.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPTLKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            complement(4915..5555)
                     /locus_tag="LGK42_004051"
                     /pseudo
     CDS             complement(4915..5555)
                     /locus_tag="LGK42_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5697..5891
                     /gene="tnpA"
                     /locus_tag="LGK42_004052"
                     /pseudo
     CDS             5697..5891
                     /gene="tnpA"
                     /locus_tag="LGK42_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
     gene            complement(6020..6619)
                     /locus_tag="LGK42_004053"
     CDS             complement(6020..6619)
                     /locus_tag="LGK42_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660227.1"
                     /translation="MSHVAKNHYEFKDITSQSPRDAVIELNERFREHGVGYQFESEEI
                     IRIDSQLIHADVVKPTLILLGGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFES
                     IMKAIHDKNNWKYSPNDTASKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKN
                     AGHGQGADIKEVPEELASYMLHLTATNLLFLLKCEKNIK"
BASE COUNT         1839 a         1535 c         1595 g         1667 t
ORIGIN      
        1 gatcattatt atcaccgttt tgttcacctt tctgagaagc tctgatgtgc cagaatatat
       61 tactgccccg gtgcgcaaag gcgatataga aaattctgtt ctggcgacag gccgtattga
      121 tgcaattgaa cgggtaaacg taggggcaca ggtatcaggg caattaaaat cactgaaagt
      181 gaaacagggc gaccatgtga ctaaagggca gttgattgcc gaaattgacg acctacctca
      241 gtgtaacgat ttgcgtaatg ccgaagctgc actcaacgaa gtgaaagcag aacttcaatc
      301 caagcaggcg ctcctgaaac aggctgaact gcgttttaaa cgccagctcc ggatgctaag
      361 agaaaatgcc agctcacacg aagattttga gtctgcggag gcaatgcttg cgaccactcg
      421 cgctgaatta ctgtctctga atgccaaact ggtccaggca cagatagaag tagataagaa
      481 aaagcttgcc cttgaatata cgcgagtcgt agcgccaatg gatggaattg ttattgccat
      541 tgtcactcag caagggcaaa ccgttaactc aaaccagagt gccccaacaa ttattaaact
      601 cgctcgtctg gatgtcatga ccattaaagc acagatctcc gaggctgata taacccgtat
      661 ttctgtgggg caaaaagccc gcttctctat cttctccgaa ccggacaagc actacagcgc
      721 aacactgcgt gccgttgaac tggcaccaga atcggtaatg aaagatgact ctctcgccag
      781 caacacttca gcatcaggct ccggaacttc aaatgcgtcc gtttattaca atgctctatt
      841 cgatgtgcct aacccagaaa atcgacttcg tattgccatg actgctcagg ttaccctgat
      901 tactgatgaa gcccaaaata cactgctggt acccattcag gcagtgcaca gaaatgaggg
      961 gaagaagcaa caggtcctgg tgctggcagc agatggcagg ctggaacccc ggaatgtgaa
     1021 aactggtatc acgaacagcg tggatattca gatcctcgaa ggtttaaatg tcggagaaaa
     1081 tgtcgtgctg tcgctgccgg ataagaaaga gcccgaagaa aggattatgc tgtgaaaaaa
     1141 cttattgagt taaaaggggt tagccgaact tatggcaacg gagatcaaac gcggaccgtc
     1201 ctgaaaaatg tcgatttgac aatcgttgct ggagaaatgg tggcaatcat tggagcctct
     1261 ggctcaggca aatcaacctt aatgaacata atgggttgcc ttgacgtacc caatagaggt
     1321 gattactaca tcgacggaca aaatgccgcc tgtctttcac ccgatgagct ggctagagtg
     1381 cgccgggaac acattggctt tatattccag cgctaccatt tgatacctga tcttagcgca
     1441 cttggtaacg tggagatccc ggccatttac gccaacagcg aacgcgacag ccgtcggcaa
     1501 cgtgcaacgg cgttgctggg cagactggga ctggaaggac gtgagcatca taaaccttgc
     1561 gaactttcag gcggtcagca gcaacgcgtc agtattgccc gagcactgat caacggtgga
     1621 aagataattt tggcggatga gccaactggt gctctggatt cgcaatccgg gcaagaagtg
     1681 ttagccattc tgaatgaact taatcgacgt ggtcatactg tagtaatggt gacccacgat
     1741 atgaaggtcg cccggcacgc aaagcgaatc attgagctgt gtgatggcga aatcatcgct
     1801 gatagtggag gttgcgtatc tgcgacggaa acactaccaa aaaccaacag aatccggcaa
     1861 agctattgga aaacactgct cgatcgaacg cgtgaatcga tgcaaatggc gctaaaagcg
     1921 atgaagacgc atcgcttacg caccacgctt accatgatcg ggattgtttt tggtatcgcc
     1981 tccgttgtca ctgttgtggc attgggtgaa ggagccaggc aagaaacact ggaagagatt
     2041 aaaagtctgg ggacaaacgt ggtcagcatt tatcccgggc aggatttgtt cgatgacagc
     2101 attgagagta ttagaacatt ggtacctgct gacgcaaatg cactggcgaa gcaagggttt
     2161 atcgatagcg tcagcccgga agttagcgct tcagacaaca ttcgttttct gggtaaatct
     2221 gcgatagcgt ccattaacgg cgttgggcgg gaacatttcc gggtaaaagg tattgagttg
     2281 ttgcaaggga ctacttttag agacgatcgc aacgctctac aggaagttat tatcgatgag
     2341 aatactcgta aggccatttt cgacaacaca gggttacagg ctctggggca aatagtcttt
     2401 cttggttctg taccagcacg agtggttggc atcgctaaaa gtaacaaccg tagtgatgca
     2461 tcaaatcgca ttactgtgtg gatgccttac agcacggtga tgtaccgtat tgtgggtaaa
     2521 ccagttctga ccggtattag cgtcaggcta aaagacaatg tggataacga ggctgcaatc
     2581 agcgctatct cccaactatt aacccggcgt cacggcataa aagacttcca gctttataac
     2641 tttgaacaaa taagaaagtc tatcgaacac acctcaatga ccttcagtat tttaattctt
     2701 atggtcgcat gtatctcact aatgatcggc agtatcgggg tgatgaacat catgttgatt
     2761 tctgtaacgg aacgaactca tgaaattggc gtacgaatgg cggttggcgc gagacgcagc
     2821 gacattatgc aacagtttat tattgaggcc gtattggttt gcctgattgg tggcgcactc
     2881 ggtattgcac tgtcgtatat cacaggtgcg ctctttaacg ctctggcgga cgggatattc
     2941 gcagctatat attcgtggca ggctgctgtt gctgcatttt tttgctcaac cttaatcggc
     3001 ataatcttcg ggtatctccc agcccgcaag gcagcaagga tggacccggt tatttcactg
     3061 gccagcgaat aatatatgaa aattttatca tcgttgactt tatgccttat caccgcactc
     3121 tgttcaggtt gcacaaacgt gctgaaaagt gactatcgcg ctcccgaagt gaactatccc
     3181 atcaactgga ccaagggcga tgtggacgga aatacatccc cgtttgactg ggaagaattt
     3241 aacgatccta atctcgataa ctggcttcac ctggtgatga caagtaataa cgacatagct
     3301 atagctgcac tgcggataca tcgggcgcag ctggatgcgg aaagaacagg tatcaccaat
     3361 actcctacac taaaggcggc actgagtatg gacggaaaaa aacagctgaa taactcgtcc
     3421 ggctgggcaa aaagcggttc tgccagcctt ggcaccagct atgagctgga tctctgggga
     3481 aaaattgccc gtcagcgtga tgttgctgag tgggcagtcc atgccagtga agaagatttt
     3541 cgctccgcgc gcctgatgct gctttcagag gccagcaata attactggcg tatcggtttt
     3601 gtaaatcaac aaattaccac cctccagcag agtattgact atgcaaaaga gacgctgcgt
     3661 ctggccgagg ttcgctatcg tgcaggaaat atctcgtcgc tggatgtgat tgacgcgcag
     3721 caaaacctgc tcacgcaaga gaaccagctt acagggctac aacgagaaca ttcacagcta
     3781 ctcaatcagc aggccgtttt gctcggcacc gtgcccggtt gccagatagt cgagcccaca
     3841 acgctgccaa aaggaagcct gcctaaggtc aatgcgaata ttcctgccag catactgatg
     3901 cgccggcctg acataagtgc taaagagtgg cagttgcgtg aggcgctagc tacggttgat
     3961 atcaagcgta gcgaatatta ccccaccttc aacctgacag gcgctttggg taccagcagc
     4021 gcttcgctac tggcattact gcataatccg gtcggttccg taggcgctaa cctgacgcta
     4081 ccgttcctgg agtggcgaca gcgggacata gaggtaaaaa ttgcccgtaa cgattacgaa
     4141 cagcgagtgc tggagtttaa acagctactt tataaggcaa tgagcagtat agaggacgcg
     4201 ctttcatttc gtaaccagtt gctgcttcag gagacaagac tcagggaaga actggaactt
     4261 gcacgtaagt cagagtggtt aaacgaagtt cgttaccggc atggtgcagt acgtatttca
     4321 ttctggcttg atgctcagga aaaacgtcgt caggcggagc tgcgactgga tgaaaaccgc
     4381 ttcaatcagt tgcagaatct agcaaaaatt tatcttgagt ttggcggggc atcaaccttt
     4441 ccttaagtct aattggggac aaccaggagc aataaggagt gaaaaatagc cacagctgaa
     4501 aacaccatag ccattgacat ttgagcttta cctgctcttg aaaacaaaaa atcactgtta
     4561 agcgcaatgc tattcacttc agcagagcag tattgcgtgt cacgagcatg tttaccattc
     4621 tgtgtaaata tcttttctta aaagtgatca tcaaagcagt catcaaactc gataaatatc
     4681 caccggcata gcgtaatgcc gagcagttaa ggatcaattg accgctcctt aaactgcggc
     4741 actataacgg cttctacaac agggagccgt tttcttgtgc atagaaaacc cccagctagg
     4801 ctgggggttc cggaaagctt tcagctttga gccagttatt aaggtaatga ctccaactta
     4861 ctgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccacctattt
     4921 tgagaacgac agtgacttcc tgcccagcct tgccagatgc tgcctcagat tcaggttatg
     4981 ccgctcaatg cgctgcctat atcgcttgct gataacgtgc agttctccct tcaggcgtga
     5041 ttcatacagc ggccagccat ccgtcatcca taccacgacc tcaaaggccg acagcaggct
     5101 cagaagacgc tccagcgtgg ccaacgtgcg ttcaccgaat acatgcgcca caaccgtcct
     5161 ccgtatcctg tcatacgcgt aaaacaacca gcgctggcgt gatttagcgc cgacgtaacc
     5221 ccactgttcg tccatttccg cgcaaacaat gacgtcactg cccggttgta tgcgtgagtt
     5281 taccgactgc ggcctgagtt ttttaagtgt cgtaaaatcg tgttgaggcc aacgcccata
     5341 atgcgtgcac tggcgcgaca tccgacgcca ttcatggcca tatcaataat tttctggtgt
     5401 gtaccgggct gagaggcggt gtaagtgaac tgtagctgcc atgttttacg gcagtgagag
     5461 cagagatagc gctgatgtcc ggcagtactt ttaccgttac gcaccacgcc ttcagtagct
     5521 gagcaggagg gacaactgat ggagatggaa gccacgggag cacctcaaaa acaccatcat
     5581 acactaaatc agtaagttgg caccattacc agttattaaa accccttttg atttgttaaa
     5641 acaccttgcg gtctggcaac tgcaagtgtc aaacaagaaa tcaaaagggg gtcccaatgg
     5701 ggaacgaaaa gagcttagcg cacacccgat ggaactgtaa atatcacata gtatttgcgc
     5761 caaaataccg aagacaggtg ttctacagag agaagcgtag agcaataggc tgtattttga
     5821 gaaagctgtg tgagtggaaa agtgtacgga ttctggaagc tgaatgctgt gcagatcata
     5881 tccatatgct acaatgtttg tcaaagctca gtgaaaatgt ctaatcactg gctcattata
     5941 aatgtccata tattgaaaag gccgctgcga aaatccctcc cgcagcggcc tctttagcat
     6001 aacgtcatta tgcgaaccat cacttaatgt tcttctcgca ttttaagaga aatagtaaat
     6061 ttgtagcagt aagatgaagc atatagcttg ccaattcttc cggaacctct ttaatatcag
     6121 ctccttgccc atgacctgca tttttgtttc tgatggttgg aattcctgtt tccagcattg
     6181 ttttcagact tgtgaactgg ctttgtaaat atgccggaat taaattttga cttaaacagc
     6241 tatttattag ttttgatgct gtatcattag gactatattt ccagttattc ttatcatgta
     6301 ttgctttcat tatactttca aatgatttca gacaatcatt tagacattct ttataacgct
     6361 tatgcctgta atgctcatgc gcagcaagga attcatcatt ggcgccttca aataagggtt
     6421 ctccacctaa aagaatcagg gttggcttta cgacatcagc atgaatcaat tgagagtcta
     6481 tccttattat ctcttcagat tcaaactgat aaccaacccc atgttctcta aacctttcat
     6541 ttagttcaat aactgcatct ctaggacttt gacttgttat gtctttgaat tcataatggt
     6601 ttttcgcaac gtggctcact agtatctgga agcata
//
LOCUS       DAFAVF010000201         6598 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFAVF010000201 DAFAVF010000000
VERSION     DAFAVF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.203"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            175..417
                     /locus_tag="LGK42_004054"
     CDS             175..417
                     /locus_tag="LGK42_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660228.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            531..1169
                     /locus_tag="LGK42_004055"
     CDS             531..1169
                     /locus_tag="LGK42_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBK2660229.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1166..1903
                     /locus_tag="LGK42_004056"
     CDS             1166..1903
                     /locus_tag="LGK42_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBK2660230.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1903..3999
                     /locus_tag="LGK42_004057"
     CDS             1903..3999
                     /locus_tag="LGK42_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBK2660231.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4046..4324)
                     /locus_tag="LGK42_004058"
     CDS             complement(4046..4324)
                     /locus_tag="LGK42_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660232.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4539..4949
                     /gene="psiE"
                     /locus_tag="LGK42_004059"
     CDS             4539..4949
                     /gene="psiE"
                     /locus_tag="LGK42_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBK2660233.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4993..6468)
                     /gene="xylE"
                     /locus_tag="LGK42_004060"
     CDS             complement(4993..6468)
                     /gene="xylE"
                     /locus_tag="LGK42_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBK2660234.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1642 a         1663 c         1757 g         1536 t
ORIGIN      
        1 ttttaatgtt atttatatat aagttgtagc ttatatttga tattcattcc aattatccta
       61 aaaccccatc tctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
      121 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      181 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgc gtgggctgca
      241 cctgtacagg tgggcgaagc ggcagggtcg gcagcaacgt cggtttcggc ggggagttcc
      301 tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc ggcaaccggt
      361 ggcggtgatg gttctaatac cgggaccacc acaaccacga ccaccagtac ccagtaataa
      421 agtatgtatc cccaaaataa tttgagtcat tgcatctgtg gcttgaagta tgaagggatt
      481 taccataacc acactccggt gtggttattc tgcccctctg gagaagagtc gtgaagcgac
      541 ctgcactcat tcttatctgc ctgctattac aggcctgttc agccacgact aaagagctgg
      601 gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg gacgatgata
      661 ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg caattgtttg
      721 tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc
      781 ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag
      841 tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat
      901 ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag
      961 tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc ccggttcgcg
     1021 tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca
     1081 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa
     1141 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcat tgattttgag
     1201 tgtgggggcg tcatcggtct tcgcggcggg aaccgtcaag gtattcagca atggcagcag
     1261 tgaggccaaa acgctgacgg gcgcagagta tttaatcgat ctggtaggcc agccgcggct
     1321 ggcaaacagt tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
     1381 gcggcagcag caggcgttgc tgacacggct ggcagaactg gctgcagatt ccagcgccga
     1441 cgatgccgcc gcgattaacg ccttacgcca gcaaattcag gcgttgaaag tgacgggcag
     1501 gcaaaaaatc aatcttgatc ctgatatcgt ccgcgttgcc gaacgcggta acccaccgtt
     1561 gcagggcaac tacacgctgt gggtcgggcc accaccgtcc acggtcacgc tgttcgggct
     1621 tatcagccgt cctggcaagc agccattcac tccaggtcgc gacgtggcga gctatctctc
     1681 cgggcagaac ctgcttagcg gtgcggatcg cagctacgcg tgggtggttt acccggacgg
     1741 acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg
     1801 cagcattatt tatgttggcc tcgcggactc cgtctggagt gagacgcctg atgcccttaa
     1861 cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg
     1921 cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata tcctgcgccc
     1981 attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt
     2041 atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac
     2101 tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgctacac cgacgtgcgc
     2161 acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc
     2221 ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt
     2281 gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc
     2341 tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat
     2401 gtgaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa
     2461 caggcgggat ctatcgacgg tagccagatg ttccacggtc ctgcctcact gtttggcggc
     2521 gtggaatacc agacgccctg gcaaccgctg cgcctgaaac tggagtatga aggcaataat
     2581 tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt cggtgcgatt
     2641 tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg
     2701 tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcttacaa cgataacgcc
     2761 cgcccgcaat atcaaccgca gcctcaggat gccattttgc agcattcggt ggtggcgaat
     2821 cagttaacgc tgttgaaata caatgccgga cttgccgatc cgcagatcca ggcgaaaggc
     2881 gatacgctgt atgtcaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc
     2941 cgtgctaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat ccgcattacg
     3001 gaaaatcgtc ttaacatgcc gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat
     3061 catctcgccg gagagccgtt gggccacgaa acgaagctgg cgcaaaaacg cgtcgagcca
     3121 gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccac
     3181 atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg
     3241 ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tgctgaccac cggcagcctg
     3301 tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccgcc gcaggactcg
     3361 cacttaccgc gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgt ctatgtgaat
     3421 aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctatgga
     3481 ggttatctcg aaaccatgtt tggcggtgcg ggggccgaag tgttgtatcg tccgctggac
     3541 agtaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca
     3601 aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcctactgg
     3661 acgccatctt tcgcccagga cgtgttggtg aaagccagcg tcgggcagta tctggcaggg
     3721 gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt ggttggcggc
     3781 tatgccacga tcaccaacgt ttcgaaagag gagtacggcg aaggggactt caccaaaggc
     3841 gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg
     3901 attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg
     3961 tatgatatga ccagcgaccg tagcgtaaat ttccgctaag tcatgggaaa ggtgccagtt
     4021 ttcgcactca ctggcaccct cattcttaat gacctgttga gccatcagga acgcagttca
     4081 cccccggttt acgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata
     4141 tcggttgcag gtctggttgc ctgagcgact gggccatcat tgggctggag acattgcttt
     4201 gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga agaacagaga
     4261 ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt
     4321 tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc
     4381 tgaatgggtg attttttggg gctgtgtgtt ttgccagatg cgacgttgat gggagatgcc
     4441 cacttaatca agctgaacaa aacatacaca aaaaatatag atctccgtca catttttgcg
     4501 ttatacagga agctcgccac tgtgaaggag gtactgctat gacgtcactc tctcgtccgc
     4561 gcgtggagtt tatctccacc attttgcaga ccgtactcaa tcttggcctg ttgtgcctcg
     4621 gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca tctggcagat gtgctgttcg
     4681 cgccagaaca aaccagcaaa tatgagctgg tagaaggact ggtggtttac tttctctatt
     4741 tcgaatttat cgcgctgatt gtgaaatact ttcagtccgg ttttcacttc ccgctacgtt
     4801 actttgtcta tattgggatc accgccattg tgcgcttgat catcgtcgat cataaatcgc
     4861 cactggatgt gttgatctac tcggctgcga tcctgttact ggtgatcact ctgtggctgt
     4921 gtaattcgaa acggctgaag cgggagtaaa aagacagcac gccgaactgg cgcggcgtgc
     4981 tggacaggaa gattacagcg tagcagtttg ttgtgttttc ttcgtttccg gttcccagag
     5041 ggcttccagc tcctcaaggg ttttaccttt ggtttccggg acaaatttcc acataaatag
     5101 tgctgccaga acgcccatac acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc
     5161 caccagccag gagtttttgt ccatcatcgg gaaggtccag gagacgaagt agttcgccag
     5221 ccactgggcc gccaccgcga ttgccagcgc tttaccacga atagcattcg ggaagatttc
     5281 cgacagcagt acccagcata ccggacccca ggacatggca aaggcggcaa catagaacag
     5341 catcgacagt agcgccacaa tacccgatgc ctgagtgtaa aacgcggtac cgaggctaaa
     5401 cataccgatt gccattccga gtgcgccgat aatttgcagt ggcttacgac caaatttatc
     5461 caccgtcata attgccagta cggtaaaggt gaggttgata actccgacaa taatggtctg
     5521 caacagcgcg atatccgtgc tggcccccag cgttttgaac acttccggcg cgtagtacag
     5581 caccacattg atgccgacaa attgctggaa gatggagagc ataacgccga ttacaatcac
     5641 gcccacgcca aacatcagca gacgaccacc ggttttgcgg ccatgatcca gggagtgttt
     5701 aatttcctgt actgcctgag ttgcaagcgt gttgcccata attttgcgca ggatactttc
     5761 cgcctgttct tgcttgccgc gtgacatcag ccagcgagga ctttctggca cggtatacag
     5821 cagcattaag aacagcagtg cagggataca ttccgaggca aacatataac gccagccgtc
     5881 agtattcagc cagctggcat caccggaacg ggcaataaaa tagtttacgc agtaaactaa
     5941 aagttgcccg aaaataatcg caaactggtt aaatgaaacc agttttccgc gaatatgagc
     6001 tggagccagt tccgcaatat acattggtga aagcattgag gctaaaccaa cgccaatacc
     6061 gccaataata cgataaataa caaattccgg gacataacct gccagataaa tagggactgt
     6121 gttgtccggg tttatagagg taaaaccaag ttctggccag gcagaaccta caccagaaat
     6181 aaaaaacagg acagcagcaa tcttaagtga atcacgacgg ccgaagcggt tactgcaata
     6241 accaccgagg gcaccgccga tgatgcaacc aatcagagca ctggctacgc aaaatcctaa
     6301 cagggagttg gcagcggatt cacttaagtt ttgtggagca acaaagacgg tattgagtga
     6361 ctcaacagta ccggaaataa cggcggtgtc gtagccaaat aataaaccac ctaatgtagc
     6421 gactaaggta atcgaaaata tataactgga attatactgg gtattcattc agacctgcct
     6481 tagaccattc tgatctttta tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa
     6541 ttatcatttt ttacaatgcg tttacgtaat gtcttttctg tgatcttaat tgtgataa
//
LOCUS       DAFAVF010000202         6503 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFAVF010000202 DAFAVF010000000
VERSION     DAFAVF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.204"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..960
                     /locus_tag="LGK42_004061"
     CDS             <1..960
                     /locus_tag="LGK42_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660235.1"
                     /translation="RFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDIGVVSGILAL
                     VLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLSLIMLSAMSW
                     GLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYKSLTRYVLLS
                     VCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLFFLRFSKRYA
                     FLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVLATLVEWRIW
                     FEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1153..1240)
                     /locus_tag="LGK42_004062"
     tRNA            complement(1153..1240)
                     /locus_tag="LGK42_004062"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1204..1206),aa:Ser,seq:gga)
     gene            1474..2412
                     /gene="ghrA"
                     /locus_tag="LGK42_004063"
     CDS             1474..2412
                     /gene="ghrA"
                     /locus_tag="LGK42_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBK2660236.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2467..3204
                     /gene="ycdX"
                     /locus_tag="LGK42_004064"
     CDS             2467..3204
                     /gene="ycdX"
                     /locus_tag="LGK42_004064"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBK2660237.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3228..3782
                     /gene="ycdY"
                     /locus_tag="LGK42_004065"
     CDS             3228..3782
                     /gene="ycdY"
                     /locus_tag="LGK42_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBK2660238.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3884..4375
                     /locus_tag="LGK42_004066"
     CDS             3884..4375
                     /locus_tag="LGK42_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBK2660239.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4439..5272)
                     /gene="csgG"
                     /locus_tag="LGK42_004067"
     CDS             complement(4439..5272)
                     /gene="csgG"
                     /locus_tag="LGK42_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBK2660240.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFILLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5299..5715)
                     /gene="csgF"
                     /locus_tag="LGK42_004068"
     CDS             complement(5299..5715)
                     /gene="csgF"
                     /locus_tag="LGK42_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBK2660241.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5740..6129)
                     /gene="csgE"
                     /locus_tag="LGK42_004069"
     CDS             complement(5740..6129)
                     /gene="csgE"
                     /locus_tag="LGK42_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBK2660242.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6134..>6503)
                     /gene="csgD"
                     /locus_tag="LGK42_004070"
     CDS             complement(6134..>6503)
                     /gene="csgD"
                     /locus_tag="LGK42_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBK2660243.1"
                     /translation="YRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASY
                     LITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFK
                     KIAVKNRTQAVSWANDNLRR"
BASE COUNT         1581 a         1472 c         1615 g         1835 t
ORIGIN      
        1 cgctttcagg ataaacgaaa tctgatatac atttatgcta ttggacttat tctgataggc
       61 ttttattgga aaaatgcagg atttactttt ccatcagagg atattggtgt cgttagtggg
      121 attctggccc tggtgctgta taattttatt tttgatctca aggcgtactg ggcttataaa
      181 tgcgtcacga agaatatcga tttttcgtgg tttaagaaaa agcagaacca caaaatagaa
      241 ttatttctta cacaacctct ggtggcagga tttctgtcgt taatcatgtt gagtgcaatg
      301 agttgggggc tataccagct tctaccctcg ttatatgcgc tgttcctgat ttcgttactt
      361 gggccgttgg tcatctttct gctgtttcgg atgatccgca ccagttatgt caagcaggtc
      421 gctatttcag tagcgaaaaa agtaaaatat aaaagtctga ctcgctatgt gctgctttcg
      481 gtgtgcatct caacggttgt taacctgctt actatcagcc cgttacgtaa cagtgattct
      541 tttgtgacag aggggcagtg gttaacgttt aaatcgataa ttgcattgct cattctttgt
      601 ggcgtagtgt tggcgattaa tctgtttttt ctgcgcttct ccaagcggta cgcttttctg
      661 ggcaggcttt ttttgcagga aatcgatctg tttttctcca gtgaaaatgc gttgtcgacc
      721 ttttttgcca agccgctttg gcttcggtta ttcatattgc tggttattga aatgatgtgg
      781 attacgctgg tgtcggtatt ggcaacgctt gtagaatggc ggatttggtt tgaagcctat
      841 tttttactct gctatgtacc gtgcttaatt tactattttt tccattgtcg atgcctctgg
      901 cataacgatt ttatgatggc atgtgacatg tatttccgtt gggggcattt taataagtga
      961 ggaagttaca ggaagtgacc agataacgca tatatgttct ctactatctt gcgcattttg
     1021 attgttgact gagtaaccag acagttgatg tgcacaattt cccctcgccc taacagacgt
     1081 gggcgggggc acccggtgtg tgcaatatac gaaaaaaaag cccgtacttt cgtacgggct
     1141 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
     1201 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtcgt tcctgtcttg
     1261 ctggaacggg cgctaattta gggaaatcat gacctgaggt caacaaactt tttgaaaaaa
     1321 tcgcgcgttt attcaaactt caatcaatgt gtggttttag taagcgaaat ctgctttttt
     1381 tgccaccgac cacggatttg ttatgctggt ggcctttgta gatcataacg ataagtgcga
     1441 ataaatttcg cacaacgctt ttcgggagtc agtatggata tcatctttta tcacccaacg
     1501 ttcgataccc aatggtggat tgaggcactg cgcaaagcta ttcctcaggc aagagtcaga
     1561 gcatggaaaa gcggagataa tgactctgct gattatgctt tagtctggca tcctcctgtt
     1621 gaaatgctgg cagggcgcga tcttaaagcg gtgttcgcac tcggggccgg tgttgattct
     1681 attttgagca agctacaggc acaccctgaa atgctgaacc cttctgttcc actttttcgc
     1741 ctggaagata ccggtatggg cgagcaaatg caggaatatg ctgtcagtca ggtgctgcat
     1801 tggtttcgac gttttgacga ttatcgcatc cagcaaaata gttcgcattg gcaaccgctg
     1861 cctgaatatc atcgggaaga ttttaccatc ggcattttgg gcgcaggcgt actgggcagt
     1921 aaagttgctc agagtctgca aacctggcgc tttccgctgc gttgctggag tcgaacccgt
     1981 aaatcgtggc ctggcgtgca aagctttgcc ggacgggaag aactgtctgc atttctgagc
     2041 caatgtcggg tattgattaa tttgttaccg aatacccctg aaaccgtcgg cattattaat
     2101 caacaattac tcgaaaaatt accggatggc gcgtatctcc tcaacctggc gcgtggtgtt
     2161 catgttgtgg aagatgacct gctcgcggcg ctggatagcg gcaaagttaa aggcgcaatg
     2221 ttggatgttt ttaatcgtga acccttaccg cctgaaagtc cgctctggca acatccacgc
     2281 gtgacgataa caccacatgt cgccgcgatt acccgtcccg ctgaagctgt ggagtacatt
     2341 tctcgcacca ttgcccagct cgaaaaaggg gagagggtct gcgggcaagt cgaccgcgca
     2401 cgcggctact aataaagcat caggattcct gctatccttg gcgggaattg aatacaggag
     2461 agagttatgt atcccgtcga ccttcatatg cataccgttg ccagcacaca tgcatatagc
     2521 acattgagtg attacattgc ccaggccaaa caaaagggca ttaaactttt tgcgatcacc
     2581 gatcatggcc cggatatgga agatgctccg catcactggc acttcattaa catgcgtatc
     2641 tggccgcgag tggttgatgg ggtagggatc ctgcgcggca tcgaagctaa cattaaaaat
     2701 gttgatggtg aaattgattg cagcggtaaa atgtttgact cgctggatct gattattgcc
     2761 ggttttcatg agccggtttt tgcaccacat gacaaagcga ccaatacaca agcgatgatc
     2821 gccactatcg ccagcggcaa tgtgcatata ataagccatc ccggaaatcc caaatatgaa
     2881 atagatgtga aagccgttgc tgaagcagcc gcgaaacatc aggtggcgct ggaaatcaat
     2941 aattcttcat ttttacactc acgtaagggc agtgaagaca actgtcgtgc ggtagccgca
     3001 gcggtacgtg atgctggagg ttgggtggca ttaggctcgg attctcacac cgcgtttacc
     3061 atgggggaat ttgaagagtg tcttaaaatc ctcgacgcgg tagattttcc gccagagcgc
     3121 attttgaatg tttctccgcg ccgcttactg aacttccttg aatctcgcgg tatggcaccg
     3181 attgcggaat ttgcagacct ttaattacta gtaatggaaa tatattaatg aacgagtttt
     3241 ctatcctctg tcgtgtgctg ggttcgctct attaccgcca accgcaagat cctttactgg
     3301 tgccgctgtt taccctgatt cgtgagggga aactggctgc gaactggcca ctggagcagg
     3361 atgagttact gacgcgttta cagaaaagct gtgatatggc gcaagtctct gccgattaca
     3421 atgcgttgtt tatcggcgat gaatgtgccg tgccgccata tcgtagcgca tgggttgagg
     3481 atgcaacgga agcggaagtg cgcgcttttc tttccgaacg agggatgcca ctggcggata
     3541 cgccagccga tcacatcggc acattgctgc ttgcggcttc ctggctggaa gatcagtcaa
     3601 cggaagatga gagcgaagca ctggaaacac tgttcagtga gtatctgtta ccctggtgtg
     3661 gtgcgttcct cggcaaagtg gaggcccatg caaccacgcc tttctggcgc accatggcac
     3721 cgctaacccg tgatgccatt agtgcaatgt gggacgagct ggaagaagat tctgaagagt
     3781 aaatgtgatc atcgtcactc ataactgcaa cagataattt gttgttgcat aaaatgtgtg
     3841 ctcgatctca ttcatggccg cgttttctgc tatcatgcgc ggcatgaaca tacttctctc
     3901 tattgcaatc acaacaggca ttctctccgg tatctgggga tgggtggctg tttccctggg
     3961 cttactaagc tgggcgggct tcctgggctg tacggcctac tttgcctgcc cgcaaggtgg
     4021 gctgaaaggg ctggcgatct ccgctgcaac cctgcttagt ggcgtggtgt gggcgatggt
     4081 cattatttac ggtagtgcac tggcaccaca tctggaaatt ctcggttatg tcataaccgg
     4141 tattgtcgcg tttctgatgt gtattcaggc caaacagctg ctgctttcat ttgttccggg
     4201 gacgtttata ggcgcatgcg cgacgtttgc cgggcagggt gactggaaac tggtgttacc
     4261 ttcgctggcg cttgggctgg tatttggtta cgcaatgaaa aacagcggcc tgtggctggc
     4321 ggcgcgtagt gcaaagacgg ctcaccgtga gcaggaaatc aaaaataaag cgtgaggggc
     4381 actcacgctt tcgcttaaac agtaaaatgc cggatgataa ttccggcttt tttatctgtc
     4441 aggattccgg tggaaccgac atatggcggt atttcaccag aatgtcgttc tgccgttctg
     4501 ctttgttctg caaatcccac agaccacggt cgataccatc attaatcagg aaaatgaccc
     4561 ctgtttcgat agccgacatc agacacagca taacgggttc gtttgaggtg taacccactt
     4621 ccccttccag caagcgctgg tagtcaataa agcggaaaac cccggcctga acttcatagg
     4681 aaagtatcgt cttactggtg ttcaccgagg agaggatctc accggtactc acattgacga
     4741 cgcgcaggtt cacggcaatc tgatcgagct ggtattgcgt gtctgcaccg atcccaaaat
     4801 atcttgcccc aacaccgccc gatttaacgt tgctttcata accgataatc gaaccttcaa
     4861 ccataatatt tgccgccgtt aaagattgca gcgggattcg gttattaatg gcaaccgtgc
     4921 cgttttcttg tgccgcacga ataatcttgc gctcgttaag caggttttgt aagccctggc
     4981 gctccagcag tataaaccag cgagaatctt tcaatgccgt gaccagcatt gccgtggcgc
     5041 tttgcggaac agcagtggag aagttacttg ccgggtaggg tttaaattgc ccggtttcgt
     5101 cctgaatgtt gtataccgaa acaaagatct tacccgtcgg cgctggcaga tgggtcaaat
     5161 ctttgtagct ctgagctcga ggcattaatg tcggtctggc ggcttcttta ggcggggcgg
     5221 ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc atgattattt
     5281 tccttatgaa gctggggctt aaaaatcggt tgagttattt tgtaaacccg aaacctggat
     5341 ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac tgcaattgac catcgcggtt
     5401 agcaatatca acaatataat cgttggtcac catgcggccc ggtttaccgg tattaatatt
     5461 cgacagtagc ccacctaaaa tttgtgactg gatggcctga gtaaagttat ctaacgctga
     5521 gggtgtttca ataccaaagt catcgttata gctcggatct ttataagagt tttgggcctg
     5581 agcgctattt aataaaaaag cgccattatt tgggttacca ccaaagtttg gattacggaa
     5641 ctggaaagtc atggttccag cccaacttaa tggcgaaata agcatgagta gaactactgc
     5701 atgtttgaca cgcattgcag cctccgaaca attttttatt tagaattcat catgcgccaa
     5761 atcgcccgta cttaataacg cctgatttat ctggcgacga tttagtgctt cttcagtttg
     5821 aatcagtgca aagacgacag ttttctcgaa gtctcttttc aacggaaata aaaaagtctg
     5881 gaaaataacg tcctgattga ccgttatagt gatccagctt ccccatcgtg cactgggcct
     5941 ttcattaatc gttaagttac ccgtatagtc actttcccat ttatcactaa aggctcggta
     6001 aaaatcatgg ccaatagatg aaacagtatg gtcagttaac aatcccggga cttctacctc
     6061 aacggcgtga agattccctg cggcgaacag aaattctgcc gccacaatcc agcgtaaata
     6121 acgtttcatg gctttatcgc ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt
     6181 tttttacggc tatcttcttg aaaagattat aaagatgcgt tttaaccgta ttttcgctga
     6241 tgaacaacga acgagcgatc tcgttattag acgcgccgat acgcagctta ttcaggatct
     6301 ctttttcccg atgagtaagg agggctgatt ccgtgctgtt ataacgatag ttaccagaat
     6361 gtgtaatcag gtaactggca agcttttgcg taaagtagca ttcgccgcgc aggacgcctt
     6421 gcaacccatt gacaacacgt tcttgatcct ccatggcata aaaaacgccg ttgatatgag
     6481 gccagttttc aatgtcgcgg tac
//
LOCUS       DAFAVF010000203         6479 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFAVF010000203 DAFAVF010000000
VERSION     DAFAVF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.205"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(56..352)
                     /locus_tag="LGK42_004071"
     CDS             complement(56..352)
                     /locus_tag="LGK42_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBK2660244.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(566..1852)
                     /gene="thrC"
                     /locus_tag="LGK42_004072"
     CDS             complement(566..1852)
                     /gene="thrC"
                     /locus_tag="LGK42_004072"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBK2660245.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="LGK42_004073"
     CDS             complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="LGK42_004073"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBK2660246.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="LGK42_004074"
     CDS             complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="LGK42_004074"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBK2660247.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5275..5394)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5330..5395)
                     /gene="thrL"
                     /locus_tag="LGK42_004075"
     CDS             complement(5330..5395)
                     /gene="thrL"
                     /locus_tag="LGK42_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBK2660248.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5609..6295)
                     /locus_tag="LGK42_004076"
     CDS             complement(5609..6295)
                     /locus_tag="LGK42_004076"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBK2660249.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1551 a         1790 c         1571 g         1567 t
ORIGIN      
        1 cgcgccagcg tcacatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc
       61 ggtgatgttt gcctggacca tgaccgccgt gatgatcatg atgagctttc ttatggtggc
      121 gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt
      181 tccaccagcc gtggtcgcgc cagtgaccgc catcccagta ataaccgcga ttatcacgat
      241 cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgc gctgccatgg
      301 gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact
      361 ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacggcggtt
      421 gttattctcc gcaatcctaa tctctaagtt accgcattta ttgagaattt ctcctttttc
      481 cctgtcgttt ttctccataa tttcttcata aaaaagccgg gctgcataaa agcaaacccg
      541 gcctgattga gataatgaat agattttact gatgattcat catcaattta cgcaacgcag
      601 caaaatcggc gggcagatta tgtgaaagca agggtaaatc agcacgttct gccagctctt
      661 ttggcagatc caacgtttca ccgagaatcg attccacgct ctctttaaat ttcgccggat
      721 gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat
      781 acgctacggc agcgtgcggc tccgaggtgt agcccagttc ttttaactca cgcattgtct
      841 gttgcgtggt ttcatcatcc acggctgcat aacccagctc tttcagttgc cagattttgc
      901 ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg
      961 ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc gtgcaggaaa cgtggcacgg
     1021 tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgagt gacttcgcca
     1081 gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc agctggttgc
     1141 gcgcttcctg tggcagctgc gcaacagctt caaagtagta gcaaatctgc gccagcaaac
     1201 ggctgatgtt aatcgagtta gctgagttta accccagcgc cactttcagt tcttcatcat
     1261 caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaacagttt
     1321 cgatattgcc gcccaacgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg
     1381 gatagaggat aaccactttc acattcggta aaccgtagaa agcatgagcc actgccgctc
     1441 cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcgcccgca atatgggtca
     1501 gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca
     1561 attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg
     1621 cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc accaataaac gccgagagga
     1681 tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga
     1741 attccggcag gtcatgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg
     1801 cttgcgcaaa gctgacctgc tcgttgtgat ctttcagatt gtagagtttc atttagtttt
     1861 ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt
     1921 gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacata
     1981 gagcgaacaa ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcga
     2041 ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc acggtagggt tcagcgataa
     2101 catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc
     2161 cagccagatg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag
     2221 ccctggcttc tgccgtcgag actttaatcc ccggatacgc cagcacccac agccactcat
     2281 caaaccctgg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac
     2341 caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagattcgt ccttccagct
     2401 cgcccatcaa agccagcaaa cgagtgtcat taagcggctt gccacagtgt tcattcatcg
     2461 ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg attggcatat
     2521 tcttttccag agtcatcgcc actggaattt gcttgccaag ctcctggcaa aaacgctccc
     2581 agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca aagcgtccga
     2641 ggttgttgag actgaatgtc tctgccgcct caaccgtgac tacatctccg agcaatgcac
     2701 catcaacagg tgtcaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg
     2761 aagccggggc ataaacttta accatgtcag actcctaact tccatgagag ggtacgtagc
     2821 agatcggcaa agacaccggc agctgtaacg tcattgcccg caccatatcc gcgcagcacc
     2881 aacggcagcg gctgataata gtggctataa aaggccaggg cgttttcgcc atttttcact
     2941 ttgaacagcg gatcattacc atccacttcg gcaatcttca cgcggcaggc accatcttca
     3001 tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg
     3061 gcaaagagat cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcagcg
     3121 ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag ttcacgtccc
     3181 gtttcacgag cgagaatcaa tagcttacgc gctacatcca taccagaaag atcatctcgc
     3241 ggatccggtt cggtataacc catttcccgc gccagagtag tcgcctcgga gaaactcatg
     3301 ccttcgtcta acttgccgaa gatataagaa agcgaacctg aaagaatgcc ggagaacttc
     3361 atcaattcat caccagcatt gagcagattt tgcaggttct caataaccgg taatccagcc
     3421 ccaacgttgg tgtcatagag gaatttacgc cgcgattttt ccgccgcatg acgcaacaga
     3481 tggtagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct
     3541 tcgcgcaaga agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg
     3601 accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc
     3661 tctttggctt gcgccagttc ttcctgccag ttttccagat ttaggccatg cacattggtg
     3721 agcagtgcct tcgagttggc aacaccgcag acacgtaagt cgatatgttt attcttcagc
     3781 cagctttgtt gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc
     3841 acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca
     3901 gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca
     3961 atggcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca
     4021 cgcaaggtgc gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc
     4081 agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca
     4141 gcccgcacac agtcgctttg cggaacgcag aaactgatac tgtattcgga agatgattgc
     4201 gtaatcagca ccacggaaat acgggcgcgt gacatcgcgg caaagacgcg cgccgccatg
     4261 ccgaccattc ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg
     4321 gaaatgccct tgaccggtaa ttcgtcttca tcacgactgg caccaatgag cgtacctggt
     4381 gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc gatgggggta
     4441 atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag
     4501 gacatcgact tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg
     4561 tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg
     4621 gagtagtcgg aaccgttgcg tccaagtacc accagttcgc ctttttcatt accggcggtg
     4681 aaacctgcca tcaacaccat gtgatcagcc ggaatgcggc ttgccgcaat acggcgggtg
     4741 gactcagcaa tatcgacggt agattcgagg taatgcccca ctgccagcag tttttcgacc
     4801 ggatcgataa cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatc
     4861 gacattttct cgccacggca aatcagcgca gcgttgatgc tatccgggca ctgccccaac
     4921 aaactaatgc catgcaggac atgttttatt tgggcaaatt cctgatcgac gaaagttttc
     4981 aattgcgcca gcgggaaccc cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa
     5041 atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc
     5101 atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc cacctgcccc
     5161 tgcctggcat tgctttccag aatatcggca acacgcagaa aacgttctgc atttgccact
     5221 gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa
     5281 aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac
     5341 cgttacctgt ggtaatggtg atggtggtgg taatggtggt gctaatgcgt ttcatggatg
     5401 ttgtgtactc tgtaattttt atctgtctgt gcgctatgcc tatattggtt aaagtattta
     5461 gtgacctaag tcaataaaat tttaatttac tcacggcagg taaccagttc agaagctgct
     5521 atcagacact ctttttttaa tccacacaga gacatattgc ccgttgcagt cagaatgaaa
     5581 agctgaaaaa tacttactaa ggcgtttttt atttggtgat atttttttca atatcatgca
     5641 gcaaacggtg caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt tgttgtaacc
     5701 agtcgaccag ttttatgtca tctgccactg ccagagtcgt cagcaatgcc atgactcgtt
     5761 cgcgtaaagc ttgcagttga tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta
     5821 ttaatgttgc taattgatag caatagacca tcaccgcctg ccccagattg agcgaaggat
     5881 aatccgccac catcggcaca ccagtaagaa cgtcagccaa cgctaactct tcgttagtca
     5941 gcccggaatc ttcgcgacca aacaccagcg cggcatggct catccatgaa gatttttcct
     6001 ctaacagcgg caccagttct actggcgtgg cgtagtaatg atatttcgcc cgactgcgcg
     6061 cagtggtggc gacagtgaaa tcgacatcgt gtaacgattc agccagcgtc gggaaaactt
     6121 taatattatc aataatatca ccagatccat gtgcgaccca gcgggtggct ggctccaggt
     6181 gcgcttgact atcgacaatc cgcagttcgc taaaacccat cgttttcatt gcccgcgccg
     6241 ctgccccaat attttctgct ctggcgggtg cgaccagaat aatcgttata cgcatattgc
     6301 cactcttctt gaccaaataa ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc
     6361 gaatttacaa attcttaacg taagtcgcag aaaaagccct ttacttagct taaaaaaggc
     6421 taaactattt cctgactgta ctaacggttg aattgttaaa aaatgctaca tatccttct
//
LOCUS       DAFAVF010000204         6477 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFAVF010000204 DAFAVF010000000
VERSION     DAFAVF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.206"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(33..236)
                     /locus_tag="LGK42_004077"
     CDS             complement(33..236)
                     /locus_tag="LGK42_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660250.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            314..1408
                     /gene="ddlA"
                     /locus_tag="LGK42_004078"
     CDS             314..1408
                     /gene="ddlA"
                     /locus_tag="LGK42_004078"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK2660251.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1432..1644)
                     /locus_tag="LGK42_004079"
     CDS             complement(1432..1644)
                     /locus_tag="LGK42_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBK2660252.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1904..2212
                     /locus_tag="LGK42_004080"
     CDS             1904..2212
                     /locus_tag="LGK42_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBK2660253.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2271..3365)
                     /gene="yaiW"
                     /locus_tag="LGK42_004081"
     CDS             complement(2271..3365)
                     /gene="yaiW"
                     /locus_tag="LGK42_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBK2660254.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASSVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3378..4598)
                     /gene="sbmA"
                     /locus_tag="LGK42_004082"
     CDS             complement(3378..4598)
                     /gene="sbmA"
                     /locus_tag="LGK42_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBK2660255.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4950..6107
                     /gene="ampH"
                     /locus_tag="LGK42_004083"
     CDS             4950..6107
                     /gene="ampH"
                     /locus_tag="LGK42_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBK2660256.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6108..>6477)
                     /locus_tag="LGK42_004084"
     CDS             complement(6108..>6477)
                     /locus_tag="LGK42_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660257.1"
                     /translation="LISEGNCTGYHLPAKQTITLIEQNQLWRDAFYWLAWQNRILELR
                     DVQLIGHNSYEQIRATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKG
                     GYIEMNKGKLVAINRLPSEY"
BASE COUNT         1643 a         1653 c         1571 g         1610 t
ORIGIN      
        1 aattacccac acaaaatata ggcttttaat tatcaacctt tattataaat aaaagttatg
       61 aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca tggccattta
      121 agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac atctttgaga
      181 taacgcactt tcttcaccag tacgttgcac gcttcgtcag cagaagattg caacaatctt
      241 tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt gttagacggg
      301 ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta aatcagcgga
      361 acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata aaagtcgctt
      421 cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg atgccagcaa
      481 ttatctgcta aatgcagacg atcctgccca tattgcgtta cgcccttcgg cgaccagcct
      541 tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg gtcagccgtt
      601 gctgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag atggttcctt
      661 gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc tggcttcagc
      721 agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc tgaacattgc
      781 gccatttatt actctgacgc gcgctaatcg tcacaacatc agttttgccg aagtggagtc
      841 taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg ttggtgtcag
      901 caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg agttcgatca
      961 taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag ttctgggcaa
     1021 cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt tctatgccta
     1081 cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag ccattgcacc
     1141 agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt tgggatgcgc
     1201 aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga tcaacgagat
     1261 caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc aagccagcgg
     1321 tctgggttac accgatctga tcacacgttt gattgagctg gcgctggagc gtcacgctgc
     1381 ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt cttattgggc
     1441 gttttcagtt tcttcatcgc gacgacgaat aatcagcccc gccagccaga aactaatcac
     1501 ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc ccagtaaccc
     1561 caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt gccagtcacg
     1621 gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca tccttgcata
     1681 ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata aatttaactt
     1741 aaggttgcca ttttgtgact cacaacgaag attcacgctt cgggaatatt cccaacatca
     1801 atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc ataagccgct
     1861 ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg atttcaccct
     1921 gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg gcaacattgc
     1981 ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga acctgttagt
     2041 gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc gcccacgggt
     2101 ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag taggctgcct
     2161 catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt aagatttacg
     2221 ttgccagata cggcctaaaa cgccttatct ggcatgacat tcctgtttga tcactgtttc
     2281 atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg
     2341 gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag tgatttaccc
     2401 gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc aaaagagaag
     2461 ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag ttttgccgcc
     2521 agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg aattaaatca
     2581 ccgtccagtg ccagcttcac gctgctggcc ttactgactg cgttctgaaa cgcggcatta
     2641 cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc gctatagctg
     2701 gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg actgaagact
     2761 tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg ttcggcaaaa
     2821 gcaatgctga cctgcatcgg cccaccggtg cgcacaggat taagcgaacc aaacagcgtc
     2881 tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg cttttctgta
     2941 cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat ttttagcgcg
     3001 gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat ctcttgccag
     3061 gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc ctgctgcgcc
     3121 accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact ttcaaaggtg
     3181 gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc
     3241 acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg agcctgacta
     3301 ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg attgacgcgc
     3361 gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt gtcaccatcc
     3421 agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac cagtgtggtc
     3481 catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt ggtaatctgc
     3541 gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa gaacaaaccg
     3601 aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat atagtgaaaa
     3661 tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc
     3721 gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc ctctactcgc
     3781 tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa tccggtcccc
     3841 atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc gataattggc
     3901 agctccggca catgcgcgga gagcgttacc agcaccggca gaaaggcgat caacgtcatg
     3961 atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg catggtgtct
     4021 tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt cgccatgtaa
     4081 tattcgttca tcgccgtacg ccagcggaac acatagtgac tgacaaagaa gttgttcagt
     4141 acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc gcggtaaaat
     4201 tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc atagaacggt
     4261 gcataccagg cgttgacggc gaccccgact tccaccaaaa accaggtgac gaagatgatc
     4321 agtgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat aaaccagaaa
     4381 aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa atccaacgac
     4441 cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg cgccacccag
     4501 tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa ggccgagaga
     4561 aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc taacttctta
     4621 tcgttttaac aactgctgtg tattaaccgc atgaccacta caaagcgtca gccaaatgaa
     4681 accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat aagaattgtt
     4741 tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc gcaggatcac
     4801 aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca
     4861 ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa tacgctcagt
     4921 taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc
     4981 tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa ccggagtttg
     5041 cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt gccacgggga
     5101 tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc gaaacgcgac
     5161 ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc ctcaccaaac
     5221 taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag ttaaacgatc
     5281 cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg acgcccatta
     5341 ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc ggtggcgcgg
     5401 cacatcgtcc ggtgtttgtc tggccaacgc gcgaacaacg ctggaaatac ctttctacgg
     5461 cgaagctaaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg ttcgacctgc
     5521 tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt gaagagcaga
     5581 ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat cagtgccgcc
     5641 gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg gcaattggca
     5701 gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag tatttgtcat
     5761 ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt tatcagcgcg
     5821 cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg cttggtttag
     5881 gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag acaggaggtg
     5941 gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc gcgtttgtgg
     6001 tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc aatgatctgg
     6061 tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta atactctgaa
     6121 ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc gcctttacgc
     6181 aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg atggatataa
     6241 ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga taataatgtt
     6301 gcgcggattt gttcgtagga attatgccca atgagctgca cgtcgcgtaa ttccagaatt
     6361 ctattttgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg ttcaataagc
     6421 gtaatggttt gtttggctgg tagatgatat cccgtacaat ttccttctga tattaat
//
LOCUS       DAFAVF010000205         6331 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFAVF010000205 DAFAVF010000000
VERSION     DAFAVF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.207"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(63..1112)
                     /gene="yahK"
                     /locus_tag="LGK42_004085"
     CDS             complement(63..1112)
                     /gene="yahK"
                     /locus_tag="LGK42_004085"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBK2660258.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1199..2155)
                     /locus_tag="LGK42_004086"
     CDS             complement(1199..2155)
                     /locus_tag="LGK42_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2660259.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(2152..3123)
                     /locus_tag="LGK42_004087"
     CDS             complement(2152..3123)
                     /locus_tag="LGK42_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK2660260.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3116..4600)
                     /locus_tag="LGK42_004088"
     CDS             complement(3116..4600)
                     /locus_tag="LGK42_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK2660261.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(4649..5632)
                     /locus_tag="LGK42_004089"
     CDS             complement(4649..5632)
                     /locus_tag="LGK42_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2660262.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(5886..6296)
                     /locus_tag="LGK42_004090"
     CDS             complement(5886..6296)
                     /locus_tag="LGK42_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK2660263.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
BASE COUNT         1737 a         1741 c         1393 g         1460 t
ORIGIN      
        1 actaaaaagt taagtggaga gcgaagttaa tatgttaaac cacagggtat ttattaattt
       61 tttcagtctg ttagtgtgcg attatcgata acaaaacgat atttcacatc accacgcagc
      121 attcgctcat aggcttcatt aatttgatcg gcccgaatca tctctatatc agccacgatg
      181 ccatgttcgg cgcaaaaatc gagcatctcc tgagtttctg gaatgccgcc aatcatagaa
      241 ccggctatcg cacggcgttt catgatcagg ttgaaaactt ccggcgattt atgcggtgtc
      301 gcaggcgcac caaccagcgt catggtgcca tcacgcttca gcaaggtggt aaaatcgtcg
      361 agattatgtg gcgcagctac tgtattcaaa atgaaatcga aactcttcag atgagccgcc
      421 atctcatcgg cattgcgtga gttaacaact tcatcggccc ccagggcttt tgccgcttcg
      481 cgttttgcct cagaagtggt aaatgccacc acatgcgccc ccatcgcgtg ggccagcttg
      541 atccccatat gtcccagacc gccgatgccg accacgccca cttttttacc cggcccggcc
      601 tgccagtgac gtagcggcga atacgtggtg atccctgcac acaacaaagg agccaccgcc
      661 gccagctgct cttgcgggtg acgaatacgc agaacatatc gctcatgaac gacgatctgt
      721 tgtgagtagc cgcccagagt atggcccggt tcgtccggcg tcggcgagtt ataggtgccg
      781 gtcatgtgat cacagtagtt ttccagcccg tcttcacact cttcgcaatg tttacaactg
      841 tcgacaatgc agccgacacc gaccagatcg cccggcgcat gtttttctac ctgatcacca
      901 acggctacca cacgccccac aatttcatga cccggtacgc aggggtaaac cgtccccgcc
      961 cactcggaac ggacctggtg gagatcggaa tggcagacgc cacagtaagc gatttcgatt
     1021 ttgacatcat tcggtcccgg ttcacgccgt gtgatatcca tcggttcaag tggttgttta
     1081 gcggaatatg caccaacagc tttgatcttc attgtgttta ctcctgatta gctatgtgta
     1141 tttggcgacg cctgatatgt catcgcggca aaacgcgtcc attgaatata gccaatattt
     1201 aaaccacccg atccagcccc aacatgccgc gaacctgaat gaaacagagc agcaacgttc
     1261 cccataaggc catcgtcagg taggggctta taccgagaat attgaagccg ctttcaataa
     1321 tttgcagaag aaacagcgcc agcaccatac caattatccg cccgctgccg ccgtccggat
     1381 taaccccacc gagtactgcc gcgaggattg acaccagcaa gtacgattcg ccataagagg
     1441 ccttagccga gttgagtttc gacatcatca aaaatgccgc taccgcacaa agcaaggcgg
     1501 aaatgacata aacccaaatc agcacccgac gagtgttaat gccggagtaa agtgttgctc
     1561 gttcgttaga cccaatcagg taaatggctt taccgagcgg cgttttctcc agcaatatcc
     1621 atagccccag tgccactgcg gcaaacaacc acatcggtag cggaataccg aaccattgcg
     1681 catgattcaa ccacagcacc catgagggat aattggcaat ggcgctacca cctgtcacca
     1741 gaatatttat ccctttgagc aacgtcatca tcccaagcgt tgcaagaatt ggcgacactc
     1801 gaataccagc aatcaatacg ccgttacata aaccgattat caccgccgcg ccagcgcccg
     1861 ccagtaacgt agcgacaacc gtagcaatac ccggtggata ctgagtcgcc acccacgcca
     1921 tcaccagtga acaggcgtta gcagtggcta taattgacaa gttaatgcca ccgcaaagca
     1981 tggtcaccgc catagccaac gccagaatgc ccagcacggg catttgcgaa gcgacggact
     2041 ggaaattact tatcgaccag aaaatctccg gcatagtaat gctgaacgcg gcaacagtta
     2101 gcaccagcag gccgatcaag taaaactcaa cgttattacg ccacgatttt ttcatagcag
     2161 actccggttc tgatgctgac tccacgccgt ggcactaatg ctggcaacga tgatgatgcc
     2221 ggtgatcaat gtttgccagt aagacgagac tcccaataaa tttagcccgt tttgcatcac
     2281 tgccaacaac accacgccga gcaaagtacc ggttaacgtg ccgcgcccgc cgagcaaact
     2341 agtgccgcca agcaccaccg cagccagtac tgtcagctca taacccagaa gagaatcggg
     2401 ggcgacagtc atcaccgtcc acgactgcac tacacccgca gcgccagaca tcaatcccat
     2461 atagccatag acgaaaagtt gcagtttcag cacgctaaag ccgatgcgac tggctgattc
     2521 gcggttcccg ccaagggcat aaattttgcg ccctacactg gtgaaattca tgataaacgc
     2581 tgttagcagc accaccgtaa tcatggcgat cagcggcaga ccaaggccat agtcatagcc
     2641 atcagcatcg gtgtacttga acaacataac cccctgctca aaccacggcg gaaagtcgta
     2701 cagccacaca cctttactca accataacag caggccatag aaaatattca gcgtcgagat
     2761 agtgatgatg atagaaggca cccgtaggcg attaaccagt aaggcgttaa ttaagccaag
     2821 taaaatgccg atgcagcccg ccagtgcaaa cgcgacagcg aagttgccac caattttttg
     2881 cagcaacagc accatgccgt attgcgcaat gatggtcatt gctggaaacg aaatatcaat
     2941 tccaccggaa atcaacacca caaacaaacc acaggcgaga atagtcaaca tggcatagtt
     3001 attggcgaga tcgtacaaat taccgaatgt cagaaactcg tcgctgcgcc aggcaaggcc
     3061 aacaaacagc accacaatca gtaatgccag ccagaattca tggcgttttt tcagttcagc
     3121 catttaccac ctccgcaatc ctggcctgag aactgctgtc aggcaggaag ctatgggtga
     3181 tctgaccttt ttgcattacc aaaatccggt ggctttgata ccacgcttct tcaatttcat
     3241 cgcagatcat caataccgca atgccatgag ccgccagatc gctgataatg ccgtatattc
     3301 ctgctttgtt ggcgatatca acaccgactg tcgggctatc gagaatcaac agtcgtggcc
     3361 caatcgccag ccatttggcg atcgatactc gctgcgcgtt accaccagaa agcgtattca
     3421 ccggaagatg aggatcagag actttgatgg tcaatgcctt gattagcctg tccaccaggt
     3481 cacgaacttt agtttcactc agaaagccga agcgatcttt taccttgtga aaaacagtgc
     3541 taatgatgtt gtcttcaata gattgcgcca tcaccaaacc gcgcgacatg cgatcttccg
     3601 aaacataacc aataccgtgg cggatagcgt cctggttact atgcagtgta accggctggc
     3661 cgttgatgag gatctcgcca gcatccggtc gggtcatacc aaacagactc agacataatt
     3721 ccgtgcgccc cgcaccaagc aggccaacaa tcgataccac ttcgcccgct tccacccgca
     3781 agttaatatt tagatattcc ccatgacgac ttaaattacg cacctccagc accggggtgc
     3841 tggattttcc ctgccacaac tctcgcacct gatattcaaa ctcctggccg gtcatcagga
     3901 aaccgagttg ttttgtggtc atctctgcag ccggaaaagt cccgaccagc tcaccatctt
     3961 tcagcactga aatacggtcg gaaacttcca tcacttcttc cagacgatga ctgacgaaga
     4021 ccacgcagat cccgcgttca cgcaattgat gcaccacctg taataaccct tgcacttcct
     4081 gatgggtcag cgaagcggtt ggttcatcca tcacgatcaa ccgtgcgtcc tgcgccagtg
     4141 cacggcaaat agccaccagc tgacagcggg caattgaaag ctcagaaacg agtgtatcca
     4201 gtggcaatgt gacgttaata ctggtcattg ccgcctgcgc cacctcacgc agacgacggc
     4261 ggttaacaaa cagaccgtgg tgatagtgat tcacggcgat attctcccag acactcaggt
     4321 taggaaataa agagaggtcc tgataaatca cctgaatccc ctgcgccacc gaagcggcgg
     4381 gtgtcagctt tgaccagttt ttcccaccaa gcgtaatttc cgctccctca tccgggcgat
     4441 aaacaccgga cataatttta atcaacgtcg atttaccaca gccgttttgc ccggcaaggc
     4501 agtgcacttc acccgacata aagtcgagag aaacatcgcg cagcgctcgc gtggcatgaa
     4561 acgttttgtt gatatgacga agggaaagga aagtttccat aacatcccct ggcaataaga
     4621 atcggggctt tcgccccgaa gaaatggatt aataaagtga gtcgatattg tctttgttga
     4681 ccagtaatac cttatggaaa cggataattc gcttatccat atctacgtcg gccttaccca
     4741 gattctgcat ttctaatcca ggtttgatct cttcaccctt cagcaacgtg ctggcgacgg
     4801 ctgcgagtgc gtaacctgca gaggccggat cgtaagtaat cccttcggtg atatcaccac
     4861 ttttaatcag tgatgccgcc tgtgaaggga tcatcatgcc atagactgcg actttatttt
     4921 tcgcccgttt ctctttcacc gcacgtcccg cgccaatcgg accgtttgaa ccaaaggaga
     4981 caaccgcttt caagtcagga taggttttca tcaggtccag tgtggtacga cgtgagtcat
     5041 ccacactctc ggcaaccggc atgcggcggg taacttcatg catatccggg taatgctctt
     5101 tctggtattt caccagcaag tcagcccata agttatgctg cggcacggtc aaactaccca
     5161 cgtaaatcac atagccgccc ttgcccccca tgcgtttcgc catatgctca acatattcag
     5221 cggcaaattt ttcgttatca atgatttcaa tatcccagtt agcacttggc tgaccggggg
     5281 attcgttggt cagaaccaca attccggcat ctcgcgcttt tttgaatacc ggttccagca
     5341 cgttggcatc gtttggcacg atagtaattg cattaacctt acgggcgatt aaatcctcaa
     5401 taattttaac ttgttgcgga gcatcagtac ttgaaggccc cacctgtgag gcattaacac
     5461 caaaggcgtt acccgcctca accacacctt cgcccatgcg attaaaccac ggcataccat
     5521 cgactttaga gatattcacc acgacttttt ccgctgcctg gagcggcgca gaaattagcg
     5581 cagcgcctaa taacagcgaa gacaccatat tgataacaaa acgtttattc atcatttcag
     5641 cctcattgca gggtagtcag gaagtgttcg cccaaattag cgcgctggag agaataagaa
     5701 ttttcgaata tttttatgca gaataaacag ccctatttca ggcagctatt aaaaatatgg
     5761 ttataaatat ggacaaaacg ggcagcaaca ataagtgtga cattcatcac gtaaaaaata
     5821 gcaaaataaa caaaattagc gatattttat gtgtgtgagt ttatttcgcg tagataaata
     5881 gttatctaca caacgtcaat aagggcatat tcttcaaata taacgtccat ttctggtgaa
     5941 tagcttcctt ctgcctggaa aaaacgacgg tgttcattac gccaatattc aagactgagg
     6001 tcaccttcac cttccttacg ggcaagctcc acggtgacat cagaaaagcg cagtaaatgc
     6061 aggccaatca ctctgataac acatacaggc tcaccacggc tattttctac aatcttatac
     6121 tcaccaatca tcggaaatgc gttgtcttca atacatcccg ccagcgaacc acaggaagct
     6181 gttttaacac cagtcgcaat caacgttgcg aggtgatcgg ccaacgctgc tgaatcgcca
     6241 aaagtacaaa aggaagcatg gggatatttt tgtgagaatt tttcttttat ggtcatggca
     6301 atatttttgc gattacggaa aaacaaatca c
//
LOCUS       DAFAVF010000206         6278 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFAVF010000206 DAFAVF010000000
VERSION     DAFAVF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.208"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            145..921
                     /gene="exuR"
                     /locus_tag="LGK42_004091"
     CDS             145..921
                     /gene="exuR"
                     /locus_tag="LGK42_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBK2660264.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1266..1928
                     /locus_tag="LGK42_004092"
     CDS             1266..1928
                     /locus_tag="LGK42_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBK2660265.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1932..2315
                     /gene="mzrA"
                     /locus_tag="LGK42_004093"
     CDS             1932..2315
                     /gene="mzrA"
                     /locus_tag="LGK42_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBK2660266.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2462..2830
                     /locus_tag="LGK42_004094"
     CDS             2462..2830
                     /locus_tag="LGK42_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBK2660267.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2868..3173
                     /locus_tag="LGK42_004095"
     CDS             2868..3173
                     /locus_tag="LGK42_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBK2660268.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3176..3580
                     /locus_tag="LGK42_004096"
     CDS             3176..3580
                     /locus_tag="LGK42_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2660269.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3570..3869
                     /locus_tag="LGK42_004097"
     CDS             3570..3869
                     /locus_tag="LGK42_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBK2660270.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4055..4447
                     /locus_tag="LGK42_004098"
     CDS             4055..4447
                     /locus_tag="LGK42_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBK2660271.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4517..5503
                     /gene="yqjG"
                     /locus_tag="LGK42_004099"
     CDS             4517..5503
                     /gene="yqjG"
                     /locus_tag="LGK42_004099"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBK2660272.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5796..6161
                     /locus_tag="LGK42_004100"
     CDS             5796..6161
                     /locus_tag="LGK42_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBK2660273.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1590 a         1533 c         1555 g         1600 t
ORIGIN      
        1 atacttattt tttaggaatt tataaatatt cacaatatta attcaataca ttaattcaat
       61 caccaaattt atatatgacc aatggcatac ccatgaataa agtggtatga taacttcgtc
      121 cgcattccac tccgggagac gcatatggaa atcactgaac cacgccgttt gtatcaacaa
      181 cttgccgctg acctgaaaga gcgcatcgaa cagggcgtct atctggtagg tgataaactg
      241 cctgcagaac gctttattgc cgatgaaaag aacgtcagcc gtacggtagt tcgtgaagcc
      301 atcatcatgc tggaagttga aggctatgtc gaagtgcgta aaggttcagg tattcatgtg
      361 gtttccaacc agccgcgcca tcagcaggcg gctgacaata atatggaatt cgccaattac
      421 ggtccgtttg agttgcttca ggctcgccag ctcatcgaaa gtaatattgc cgagttcgcg
      481 gcaactcagg taacgaaaca agacatcatg aaactgatgg ccattcagga acaggcgcgc
      541 ggcgaacaat gcttccgtga ttccgagtgg gatttacagt tccacattca ggtagccctg
      601 gcgacgcaga actccgccct ggcggccatc gttgaaaaaa tgtggaccca acgtagccat
      661 aacccgtact ggaaaaaact gcacgaacac attgattccc gtaccgtcga taactggtgt
      721 gatgaccacg atcaaatcct caaggcgctg attcgcaaag atcctcatgc tgctaagctg
      781 gcaatgtggc agcacctgga aaacaccaag atcatgttat tcaacgaaac cagcgacgac
      841 ttcgagttca atgccgaccg ctatctgttc gccgaaaacc cggtagtgca tctcgatacg
      901 gccaccagtg gcagtaaatg aaactcctgt ccgacaggcg ctgcgacgcg cctgtttgtc
      961 tcgtcggtaa gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc
     1021 tgcattcagc aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct
     1081 ttgttacaat tagattcaat ttgaatttat gtttttgaat gctttcttat ctcacgattt
     1141 aacagggaat agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca
     1201 ataacattgc ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat
     1261 aatgaatgga acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc
     1321 tggccaatcc atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg
     1381 aaaacggctt gcttccagcg gcctttttac cgggcgacag tttactggta ttggtcggcg
     1441 tgttgattgc gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg
     1501 ccagcctcgg ctgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg
     1561 tacaaaactg gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc
     1621 ataaacacgg tttatcggcg ctgttaattg gtcgctttat tgcgtttgtc agaacactgc
     1681 tgccgacgat tgccgggtta tcagggctga ataacgcgcg ctttcagttt ttcaactgga
     1741 tgagcggtct gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc
     1801 cggtattttt aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc
     1861 tgctggtgtt tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc
     1921 gggggtaagg gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg
     1981 ttttacttct ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta
     2041 cgctggcgat tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc
     2101 atcaccttga cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt
     2161 taattacatt tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat
     2221 tgccccatgg ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga
     2281 cccggttacg ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac
     2341 tcactttggt gatttcaccg taactgtcta tgattaatga ggcggtggtt aacactaccg
     2401 cttcgttgtc tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag
     2461 aatgaaatac cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc
     2521 cactaccctg tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa
     2581 acaccaaaac cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa
     2641 ctgttcagat agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga
     2701 ggtggcggaa cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgataagat
     2761 tgccaaacgc gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg
     2821 cgactactaa ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac
     2881 acactacgga acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc
     2941 ttagctcatc tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc
     3001 aggcactgaa acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa
     3061 cccgtgtcgc ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg
     3121 gaattggcgc tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc
     3181 ggacactcat cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt
     3241 ttccatcatg gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga
     3301 ggaaaaagct aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc
     3361 atttggtctt atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct
     3421 gaatgcgatg attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg
     3481 gacgttacgt aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa
     3541 cgatcggcaa ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta
     3601 aggcgcaact gcttagccag atccagcaac aacggctgga tctttccgcc agtcgtcgtg
     3661 aatggctgga ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct
     3721 cctgggcgct ggttggcagt agcgtgatgg cgatctggac gattcgccat cctaatatgc
     3781 tggtccgctg ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga
     3841 ccctcaagca gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta
     3901 tccctcaaat tttttgaaga tttttgacag ttttccttgc taacaatcat cattcaccac
     3961 gtttatgatt ctctccatcg acagcaacga cgctaatacc gcgcccttgc acaaaaaaac
     4021 aatcagcagc ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt
     4081 actggtagcg cgcattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac
     4141 cgcttactct ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgcc
     4201 gctggtgatt ctgcttgagt tcggtggtgg tctggcaatc ctgttcggtt tcctgactcg
     4261 caccacagcc ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa
     4321 ctttgctgaa ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt ctggcggatt
     4381 cctgctgctg gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc tgaataaaaa
     4441 gtggtaagca cgcgctatac ttaacgataa aaaacgagga ggaagctcct cgtttttgct
     4501 attggaggag agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca cctggtacga
     4561 taccaaatct accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac
     4621 tgccgatggc gcacctggcc ccactggcac aggcggtttt atcgcagaga aagatcgtta
     4681 tcatctctat atttcactcg cctgcccgtg ggcgcaccgc acgctgatca tgcgcaaact
     4741 caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg
     4801 ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc aacatgaatt
     4861 tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta ctgttcccgt
     4921 gctgtgggac aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa tcatccgcat
     4981 gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc caccagccct
     5041 gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca acggcgtgta
     5101 taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga
     5161 atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg gcaaccagct
     5221 aaccgaagcc gatattcgcc tgtggaccac gctggtccgt tttgatccag tgtatgtgac
     5281 ccacttcaag tgtgataagc accgcatcag cgattacctt aatctgtatg gcttcctgcg
     5341 tgatatctac cagatgccgg gaattgccga aacagtcaat ttcgatcaca tccgtaatca
     5401 ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg gtccatggca
     5461 ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg caccttacga
     5521 ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg tgaacgcctt
     5581 atcaaaccta caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc
     5641 gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcgccttca cagcacttta
     5701 ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta atttgctgct
     5761 ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg aaagtactaa
     5821 aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg
     5881 tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc tggcaacgcg
     5941 ctggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta ccgtggtggg
     6001 cagttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca ctgttgttct
     6061 taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg ggtacgccag
     6121 gcgaaaaccg ctttggacca gatccgaaac tggaacctta aaacaaatct gctcgccgga
     6181 tgtctgactc atccggcttt tatcatcatg ttgatgaatg aataatttac taatgtttat
     6241 ttaaaatatt tcaatgtcgt tatataaatt acctataa
//
LOCUS       DAFAVF010000207         6228 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFAVF010000207 DAFAVF010000000
VERSION     DAFAVF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.209"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(265..1935)
                     /gene="betA"
                     /locus_tag="LGK42_004101"
     CDS             complement(265..1935)
                     /gene="betA"
                     /locus_tag="LGK42_004101"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBK2660274.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1949..3421)
                     /gene="betB"
                     /locus_tag="LGK42_004102"
     CDS             complement(1949..3421)
                     /gene="betB"
                     /locus_tag="LGK42_004102"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBK2660275.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3435..4022)
                     /gene="betI"
                     /locus_tag="LGK42_004103"
     CDS             complement(3435..4022)
                     /gene="betI"
                     /locus_tag="LGK42_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBK2660276.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4151..6184
                     /gene="betT"
                     /locus_tag="LGK42_004104"
     CDS             4151..6184
                     /gene="betT"
                     /locus_tag="LGK42_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBK2660277.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1257 a         1729 c         1740 g         1502 t
ORIGIN      
        1 accgtgagcg ggagtgaaca agtcaccata gccaggaaca ctagcggcgt aattcttaag
       61 ctgagtggca tttcacctgc ggtttatcag tgcattcata gcaccttcat cagccagatt
      121 gaacgagtcc gcttagaatt tgctggcgag gttgtcacta tgaagcatga gagttacccc
      181 tatgttttat ataacgattc tgtccccata cctggaacgg taactctcaa tcagatcggt
      241 tcttgagtca gttcaaatca cgactcattt tttcgctctc accggcatcc catttgccac
      301 aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca cgaatcatat ccgctatttt
      361 ctcgccaatc ataattgtcg tggcgttcaa attcccggtg ataatctgcg gcataatcga
      421 cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg ccttcgccgt caaccacgga
      481 catctcgtcg taacccattt tgcaggtacc gcacggatgg aaggcggttt cggcgtggtt
      541 acgcacgaac tcatcgagct gttcatccgt ctgacattcc gtgccggggc tgatttcgcg
      601 gccacgatac tgatccagcg cgggttgatg catgatctcg cgggtgatgc gaattgcgtc
      661 gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaatcg ccggatgctg
      721 gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca ttgagccgac
      781 gtggcactgg aaaccgtgct ctttcactgc attcgagccg ttatagttaa tcgctactgg
      841 caggaaatgg tactgaatat tcggccacgc aaattcctca cggctgcgaa taaatccacc
      901 tgcttcaaag tggttgctgg caccaacgcc agtgccgcca aacagccact ccgcaccgat
      961 tttcggttgg ttccaccact gcagtgcagg gtagagggaa accggttctt tgcactcata
     1021 ttgcagatac atctccagat gatcctgaag attttcgccg acgccgggta attcatgcac
     1081 cagcggaata tcaaactccg ccagcagttc agcgttgccg acgccggagc gttgcaggat
     1141 ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct ttgttggccg ttgcgcgggt
     1201 tgggatggtg ctgtcgcctt ccagccattc gacgcccacc gcgcgtttgc cgtcaaaaat
     1261 gatgtgatcg gtcatagcgt gagtacgaat ggtcaggtta gggcgcgatt tggcctgatc
     1321 gagataaccg cgcgcggtac tggcgcgacg gccctgcggc gtgacggtgc gatccatcgg
     1381 gccaaaacct tcctgctgat agccgttgag atcgtccgtg cgcgggtagc ctgcctgcac
     1441 gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg tggtgacgct
     1501 caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca tcgcgagtct cggccttgcg
     1561 gtagtagggc aggcagtcga gataactcca gttctccaga ccgggttctt gcgcccagtt
     1621 atcgagatcc agcgcattgc cacggatgta gcacatgccg ttgatcagcg acgatccacc
     1681 caggccttta ccgcgtccgc actccatgcg gcggttattc ataaacggtt caggctctgt
     1741 ttcataggcc cagttgtagc gtttgccctg tagtgggaac gccagcgctg cgggcatctg
     1801 ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca agcagcagca cggaggtatt
     1861 cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac caataatgat
     1921 gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta gccatctcaa
     1981 cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc tcgcgaccaa
     2041 tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc caggtgttga
     2101 tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc aggtccgccg
     2161 tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc gcggcgaatc acttcgtcct
     2221 cagattcata agtgagaagc gacatcactg gcccgaagat ctcttcacgc acgatggtca
     2281 tatcgtcgct gcaatcggtg aacactgtcg gtgcaaccca tgcgccgtta tcgaagccat
     2341 cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg cctttggcga
     2401 tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggccgaag ttagtttgcg
     2461 gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaattttc tgctcaaatg
     2521 cggctttgca tttcgccgga acgaagacgc gggtgccatt ggtacacacc tgaccggagc
     2581 tgaagaagtt tgccatcatg gcgatatcgg cggcgagatc gagatccgca tcatcgaaaa
     2641 cgatcagcgg tgatttaccg cccagttcca tggtcacttc tttcagggaa gaggccgccg
     2701 agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact ttggcaatgc
     2761 ccggatgctc ggtcagatat tgcccggtct ccgcgcccac gcccggcaac acgttaaata
     2821 cgccgtccgg caggcccgct tcgctgtaaa tttcagccag ctttaacgcg gtaagcgggg
     2881 taacttcgct cggtttgaaa atcattgcgt tgcctgccgc cagcgccggg gcggatttcc
     2941 acagggcaat ctggatcggg tagttccatg cgccaatccc tgccactacg cccagcggtt
     3001 cgcggcgggt atacacaaag gacgtttcac gcaacgggat ctggctgcct tccagcgccg
     3061 ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt aacgatatcg acggttgagg
     3121 tttccgaata tgcttttccg gtgtcgaggg tttccagttt tgcgagttcg tcattgcgtt
     3181 cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg gtcatcgccg
     3241 cccagatttt ttgcccctgt tgggcactct tcacggcgcg atcgacatcc tcacgcccgg
     3301 cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg aaggtgcgac
     3361 cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc attcgggaca
     3421 tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg ggtcagcgag
     3481 tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca taatccgtca
     3541 atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa ctcgcggcga
     3601 aattcgctca ccagattcga caataagcgg cggctactga cctgctgtaa acgatagagc
     3661 atcggctgat gcatactgct ggcccagaac gccagccagg ctttcatcgc cgcactgctc
     3721 acctgcgttt catcgaagtt tccgccaaca atcgcctgta atcgctgctc tgcactgccc
     3781 tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt gatatcgcgc
     3841 atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat ccccgtagaa
     3901 acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac ttcatttatt
     3961 gcttccagtg tggcgtcgat cagttgtctg cgccggatcg actgcatccc caatttgggc
     4021 attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt gattggacgt
     4081 tcaatataaa atgtgtctta attgttacga atttgatttt aaatggtaac aataacagtg
     4141 gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt
     4201 gttttacacc tccgccggac tgattttgtt gttttccctg acaacgatcc tgtttcgcga
     4261 cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct tcggttggta
     4321 ctatctactg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt gttcgcgttt
     4381 tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc tgagttgggc
     4441 ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc
     4501 ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca
     4561 ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat
     4621 gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca tccgctcggc
     4681 gctgtacccg atcttcggta aacggattaa cgggccgata gggcactcag tggatattgc
     4741 agcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg tggtgcagct
     4801 taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcaa aagcggcact
     4861 gatcgccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg ataaggggat
     4921 tcgtgtgtta tcggaactta acgttgcgct agcgctggga ttgatcctgt tcgtattgtt
     4981 tatgggcgac acctcgttcc tgcttaatgc gctggtgctg aatgttggcg actatgtgaa
     5041 tcgctttatg ggcatgacgc tcaacagttt tgccttcgac cgtccagttg agtggatgaa
     5101 taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg tcggcttgtt
     5161 cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca cgttgattat
     5221 tccgtttacc ttcacgctgt tatggctctc ggtgttcggc aatagcgcgc tgtatgaaat
     5281 catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta
     5341 cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg
     5401 cctgctgttt tatgtgacct cggcggactc cggtgcgctg gtgctgggga atttcacctc
     5461 gcagcttaaa gatatcaaca gcgacgcccc cggctggctg cgcgtcttct ggtcggtggc
     5521 gattggcctg ctgacgctcg gcatgctgat gaccaacggg atatccgcgc tgcaaaacac
     5581 cacggtgatt atggggctgc cgttcagctt tgtgatcttc ttcgtgatgg cggggttgta
     5641 taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata ccgcgccgcg
     5701 accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta
     5761 tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg caatggaaga
     5821 agtggcgcag gagctgcggt tgcgcggcgc gtacgtggag ctaaaaagcc tgccgccgga
     5881 agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag aacaaaactt
     5941 tgtctatcag atttggccgc agcaatattc ggtgccgggc tttacctacc gcgcacgtag
     6001 cggtaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc agggcaacga
     6061 cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt acgagcggca
     6121 ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc
     6181 gtgattgtta ttgcataaaa ccgcgccatg tctgcatatg gcgcggga
//
LOCUS       DAFAVF010000208         6214 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFAVF010000208 DAFAVF010000000
VERSION     DAFAVF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.210"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(111..263)
                     /locus_tag="LGK42_004105"
     CDS             complement(111..263)
                     /locus_tag="LGK42_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBK2660278.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(528..1262)
                     /gene="cysH"
                     /locus_tag="LGK42_004106"
     CDS             complement(528..1262)
                     /gene="cysH"
                     /locus_tag="LGK42_004106"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBK2660279.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1336..3048)
                     /gene="cysI"
                     /locus_tag="LGK42_004107"
     CDS             complement(1336..3048)
                     /gene="cysI"
                     /locus_tag="LGK42_004107"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBK2660280.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3048..4847)
                     /gene="cysJ"
                     /locus_tag="LGK42_004108"
     CDS             complement(3048..4847)
                     /gene="cysJ"
                     /locus_tag="LGK42_004108"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBK2660281.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5166..5528
                     /gene="queD"
                     /locus_tag="LGK42_004109"
     CDS             5166..5528
                     /gene="queD"
                     /locus_tag="LGK42_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBK2660282.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5568..6122
                     /locus_tag="LGK42_004110"
     CDS             5568..6122
                     /locus_tag="LGK42_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBK2660283.1"
                     /translation="MLGCCVLDIWGMTWKTTATLLLLVPVLQAPLARYAWIYRSILES
                     VALMLKNNYDNMRNEMNHFAKHVIITGGGSNSDLFMQIFADMFNLPARRNAINGCASL
                     GAAINKAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRYDAMNKGIFKDLTKHNDVI
                     LKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         1436 a         1658 c         1593 g         1527 t
ORIGIN      
        1 aataataaat agagactata tattcacata aaaaatataa attttaaaaa agggtgcagg
       61 agaccaaacc atcacaattc agtacgcggg gttttacccc cgcgtggtgg ctacttcttc
      121 gattcgtaag cgagaactgc cttaaactcc ataccacgtt ctctgatact caactcacac
      181 aacgagtcac ctaccatcag cgtgaatccc agtactgtac aacacagtac gatgattgcc
      241 acaagggcat attttgtcag catgtcttgc ctccctgcaa agaagagact aacattcgaa
      301 ttgtttgggt tcgaacgtaa gcctcaggtt gatagaaata tcgcctgggg cttttgtcca
      361 tctggaacct cgcgaatgct taacgccaga cagcctcaag cacccgacgc cattctatac
      421 ctgataattc ttccagcggc gttcacgttt aaaatcagga atactgctcg caaaataaaa
      481 acgcccccat catttctgac agaggcgttt aatttatccg gcaatattta cccttcgtgc
      541 aatccacatt ccctttttaa gccaaagaaa cgtgtttctt cttccgacat gccaggttcc
      601 catttacggg ttgtatgggt atcaccgacc gataaatatc cttcatccca taatgggtga
      661 tatttcaggc catgtttttg caggtactga taaatagttc ggttatccca gtcgataatc
      721 ggcagcactt taaatacgcc acgctgaatt gccagcaccg gtaaattggc gcggctgccg
      781 gattgttcgc ggcgcaggcc agcaaaccac gtttgcgcat tcagttcttt cagcgcccgg
      841 ttcatcggtt cgactttgtt gatgtcattg tacttttcaa tgccttcaac gccctgttcc
      901 cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt cggtagcgcg gtacactttc
      961 aggttgagct tgagtttgtc cgttaactcg tcaataaagc ggtaggtttc cgggaacaga
     1021 tagccggtgt cggtgaggat caccggaata tccgggtgaa tctgattcac cagatgcagg
     1081 ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat attcaccggg cagattatcc
     1141 agcgcccagg caacgcggcc ttcagcgtcc agtttttcca gttcggcgtt agtttctgcc
     1201 agcgccagaa tgcgatctac cttcggcagt tcgttcaggg cgtttagatc gagtttggac
     1261 atagattcct cactgtttgc cttgcctgat gcgacgctca cgcgtcttat caggcctaca
     1321 agaccgggct gatggttaat cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat
     1381 gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg
     1441 ccctatcagt tcatcaagcg acgccaggat ttccggctcg gtgatgtttt ctttatacat
     1501 ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc agattgtagc gacccggtgc
     1561 tttacccacc aggcccactt ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt
     1621 tacacgcatc acgatatgct catcgctgac accatgtttc gccattaaat tatcgatgtt
     1681 gtcgataaaa gacggcagga aacgctctgc ttccgccatc gccagcgggc aagtcgggaa
     1741 tgacacgcag gccatcgagt tttcacgctg cggcgtgacg gcattcatta acccgctctc
     1801 tttggcgatc ttctcgatct tcgctttctc gctttccggt acaccggcga tgatcagatt
     1861 ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc gcgatctcca gcaggccggt
     1921 tttcagcgga cgccccggat aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg
     1981 ccagttatca tcaatgccct taacccagcc aatacgatcg cctcgtccgg tgaactcata
     2041 tggacggatc ggttcaaatt tgatccccgc gcgacgctcc acttccgctt taaacgtctc
     2101 aacccccacg cgctccagcg tgtatttggt tttggcattt ttacgatcgg ttcggttacc
     2161 ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc agcgtatgct ccagcggcag
     2221 atagccaaac tcgctcgccg tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag
     2281 cccaccgccc accagcaggt tgaagcctac cagcttgccg ttttcggcga ttgccacgaa
     2341 gttcatgtcg ttggcatgca gatcgatatc gttctgcggc gggatcacta ccgtggtttt
     2401 gaacttacgc ggcaggtagg tctgaccaag gatcggttct tcatcagtag tggcgacttt
     2461 ttcctggtcg agccagatct ccgcatacgc gcgggtacga ggcaacagat gctcagaaat
     2521 cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca
     2581 gagtacgtta cggttcatgt cgttagctgt cgccagcgca tcaagaccga ccgagtgcag
     2641 catctggtgc accggtttga cgttcttttt cagaatgccg tggaactgaa acgtctggcg
     2701 gttggtcagg cgaatgctgc catagatggt gttttcaccg gcaaatttat cgatcgcttg
     2761 ccactgtttg gtggtgatca cgccacccgg cagacgacag cgcaacagca tcgcatggcg
     2821 cggctccagc ttctgttcag cacgttcggc gcggatgtcg cggtcatcct gctgatacat
     2881 gccgtggaag cggatcagca ggaagttatc gcccttaaag ccgccggtca gaccgtcgtt
     2941 taaatcttcc gcaatggtgc cgcgcaggta gttgctttca agcttcatgc gctcggcgtc
     3001 tgtcagttta ccttcgacca ctaaaggccc tggatgtttt tcgctcatta gtagacatct
     3061 cgctgataac ggcgctctac gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc
     3121 atgccaccaa attcggcaat cacttccaga agtgcctgct caacgtcttt cgccatgcga
     3181 ttagcgtcac cgcagacata aatatgggca ccgtcattga tccagcgcca cagctccgcg
     3241 ccctgttcgc gcagtttgtc ttgtacgtaa actttttctt tttggtcgcg cgaccaggcg
     3301 agatcgatcc gcgtcagcac accctctttg acgtagcgct gccactccac ctggtagagg
     3361 aaatcttccg taaagtgcgg gttgccaaag aacagccagt ttttacccgg tgcttcgtcg
     3421 gcagcacgtt gctgcataaa ggcgcggaac ggcgcgatgc cggtgcctgg gccaatcata
     3481 atcaccgggg tttccgggtt agcgggcagg cggaagttat cgttatgttc gataaatacg
     3541 cggagttcgc cctcttcttc cacgcggtcc gcgaggaagc tggaggcacc accggcacgg
     3601 gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga cgtgtacttc gttctcgact
     3661 tccgcctgcg aggaggcgat ggaatacaga cgcggcgtca gcgggcgcag cagattaatt
     3721 agcgcttcgg catcaagctg tgccggggag aaacgcacca tgtcaacaat cggcgtcgtc
     3781 gcggcgtaat gctgtaactt cgctttatcg cccaccagcg gcagcagtgt ttcactgcgg
     3841 gtaagcgtgg cgtaattctc aacaatgttg gcggtgttga cggtcagttc gaagtgccac
     3901 tgtagcgctt cgttcagagg caacgttttg ccctcgacgg tgacaggttc atcgcctgtc
     3961 agccacagca gttcgacaag ttctttcacc agtgccggat cgttctgata ccagacgccc
     4021 agcgcgtcac ccggctggta acgcaggccc gagtcaccta agtcaatttc gatatggcga
     4081 acgtcttttt cagagttacg cccggtaatt ttctggttaa cagagaggct agccaccagt
     4141 ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta ccgcgccagt agcgacggat
     4201 tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac
     4261 tcgctggcag cagcctggta ttcaacatcg gcatcgacac ggtcgagcag gcgttcaccg
     4321 cccagttccg ccagcttgct gtcgaaatct ttcccggact ggcagaaaaa ttcataagag
     4381 ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca gctttggcgc ttttttggag
     4441 aacaggaact tatgcagcgc gacggcttct tccggcggtt ccccttcccc ttgcgttgac
     4501 gtcaccacga taagcagttt ttcgctggcg atttgtttga atttatagtc gcccgcgttc
     4561 accagcttaa cgttcagttt tgccgtcaat aaatcatcgc gtaatgcttc agcaacccgg
     4621 cgcgcattgc cggtttgcga agcagagata atagttatac ccggcatttc tgcggctggc
     4681 gctggcgtcg ctgcaagcgc agcaggctgc tgattgagca cgccccagaa atagccagaa
     4741 acccaggcaa gctgggtggg agttaaatcg gtcgtggccg cctgaaggcg tgccagttgc
     4801 tccgggttca acggaagcaa cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt
     4861 ccagtaagca aagctgtttc tgcgccctgt cagcgcccat aaaacagaag agaaggtaag
     4921 gttaacgggg caaatggtgt ggattaaaga cgggatagcg ataactaata accaaatcga
     4981 ctaacctgtt ttagcaatag tctttaacaa caaaatagat taaccaaccc aatgaaaaac
     5041 aaatgaattt agccaatcat taagataaat cagcgatttt gcgcaacaaa gtcgttttag
     5101 ataatgcgaa aaaacagcct ttccggtact ctacggcggt tttatcgtcc tgtagagaaa
     5161 ttatgatgtc caccacgtta tttaaagatt tcaccttcga agccgctcac cgcttaccac
     5221 acgtcccgga agggcataaa tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg
     5281 aaattaccgg ggaagtcgat ccgcatacgg gctggattat cgatttcgct gaactaaaag
     5341 cggcgtttaa accaacctac gagcgcctcg atcaccatta tctcaatgat attccaggtc
     5401 tggaaaaccc aaccagcgag gttttagcaa aatggatttg ggatcaggtt aaacccgttg
     5461 tgccgctgtt aagtgcggtg atggtaaaag aaacctgtac cgcaggttgt atctatcgcg
     5521 gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag taacgctatg ttagggtgtt
     5581 gtgttctgga tatctggggc atgacatgga agacgactgc gacattatta ttattggtgc
     5641 cggtattgca ggcaccgctt gcgcgttacg catggatata tcgttcgata ctggaaagcg
     5701 tggctctgat gctgaagaac aattacgaca atatgcgtaa tgaaatgaat cactttgcaa
     5761 agcatgtgat cattactggc ggcggttcga acagcgatct gtttatgcag atttttgccg
     5821 acatgttcaa ccttccggca cgacgtaacg ccattaacgg ttgtgcaagt ctgggggcag
     5881 cgattaataa ggcggtaggt ctgggactat acccggatta cgcaacggct gtcgacaaaa
     5941 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
     6001 tgaataaagg cattttcaaa gacctaacca aacataatga tgtaatactg aaaaaatcgt
     6061 atgaagtgat gcatggggaa ttggggaatg tggattcgat ccagagctgg tcgaatgcgt
     6121 aagtgaggaa ggccgggcgg gaaactgccc ggcctgaaca tacctgaatg gttatccccg
     6181 ctgacgcggg gaacataagt tcgacaactt aaaa
//
LOCUS       DAFAVF010000209         6169 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFAVF010000209 DAFAVF010000000
VERSION     DAFAVF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.211"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1367
                     /locus_tag="LGK42_004111"
     CDS             <1..1367
                     /locus_tag="LGK42_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2660284.1"
                     /translation="LGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFY
                     QTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYSKR
                     GQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNN
                     IWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLL
                     PDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAYGNTNVGYSRNGDSSQIYYGMSGG
                     IIAHADGITFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRV
                     ALNANSLADNVELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVT
                     HGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPEVSPGTLLNQQT
                     AICR"
     gene            1381..1911
                     /locus_tag="LGK42_004112"
     CDS             1381..1911
                     /locus_tag="LGK42_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2660285.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            1924..2427
                     /locus_tag="LGK42_004113"
     CDS             1924..2427
                     /locus_tag="LGK42_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBK2660286.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAVDVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            2486..3400
                     /locus_tag="LGK42_004114"
     CDS             2486..3400
                     /locus_tag="LGK42_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2660287.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            3734..6013
                     /gene="ydeP"
                     /locus_tag="LGK42_004115"
     CDS             3734..6013
                     /gene="ydeP"
                     /locus_tag="LGK42_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBK2660288.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICHVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT         1722 a         1369 c         1454 g         1624 t
ORIGIN      
        1 accttggtat tgggaaagat ttaggacttt ttggtgcctt ttctttcgat atcacgcagg
       61 ccaatacgac acttgcagat ggcacccgtc acagtgggca atcggttaaa tccgtctaca
      121 gcaaatcctt ctaccagaca ggaaccaata tccaggtcgc aggatatcgc tattctacgc
      181 aaggttttta taacttatcc gacagtgcct acagtcgaat gagtggttac accgtcaagc
      241 ctcctaccgg agacaccaat gagcagacac aatttattga ttattttaat ctgttctaca
      301 gtaagcgtgg tcaggaacaa ataagcatct ctcagcagct tggaaattac ggtacgacat
      361 ttttcagtgc cagtcgccaa agttactgga acacgtcacg cagcgaccag caaatatcat
      421 ttggattaaa tgtgccgttt ggtgatatta cgacttcgct gaattacagc tattccaata
      481 atatatggca aaacgatcgg gatcatttac tcgcttttac gcttaatgtt cccttcagtc
      541 attggatgcg tacagacagt cagtcggcat ttcgtaattc aaacgccagt tacagtatgt
      601 caaacgattt gaaaggcggc atgaccaatc tatcgggggt ttatggcact ctgctgccgg
      661 ataataacct gaattatagc gttcaggtcg gtaacaccca cggaggtaat acatcgtctg
      721 gcaccagtgg ttacagttct cttaattatc gtggagctta cggtaatacg aatgtcggtt
      781 acagtcggaa tggtgacagc agccagattt attacggaat gagtggtggg attattgctc
      841 atgctgatgg catcaccttt ggacagccgc tgggcgacac aatggttctg gttaaggctc
      901 ctggcgctga taatgtcaaa atagagaacc agaccggaat tcataccgac tggcgtggct
      961 atgcaatatt accatttgcg acagaatata gagaaaatcg tgtcgctctt aacgcgaatt
     1021 cccttgcaga taatgttgaa ctggatgaaa ccgtagtcac tgtcatccca actcacggtg
     1081 ctattgccag agcaacattt aatgcacaaa tcggcgggaa agtattaatg acgttgaagt
     1141 acggtaataa aagcgttcca ttcggtgcaa ttgtcactca cggagagaat aaaaatggca
     1201 gcattgtcgc ggaaaatggt caggtttatc tgactggact tccacagtca gggaaattac
     1261 aggtttcatg gggcaatgat aaaaactcaa actgtattgt cgattacaag cttcctgaag
     1321 tctctcctgg aaccttgctg aaccagcaga cagcaatctg tcgctaagga acaatagatt
     1381 atgaaataca ataacgttat tttcctcggt ttatgtctgg ggttaaccac ctattctgct
     1441 ttatccgcag atagcgttat taaaattagc gggcgcgtcc tcgattatgg ctgcacagtc
     1501 tcatcggatt cgcttaattt taccgtagat ctccaaaaaa acagtgccag acaatttcca
     1561 acgaccggta gcacaagtcc agccgtccct tttcagatta cgttaagtga atgcagcaaa
     1621 gggacaacgg gggttcgggt tgcatttaac ggtattgagg atgcagaaaa taatactctg
     1681 ttgaaactgg atgagggaag caatacggcc tccggtttag gtatagaaat actggacgga
     1741 aatatgcgtc cggtgaaact gaatgacctt catgccggga tgcagtggat cccactggta
     1801 ccagaacaga acaatatttt gccttactcc gctcgtctga agtcaactca gaagtccgtc
     1861 aatccgggac tggtgagggc ttcggcaacc tttacccttg aatttcaata atcagagtca
     1921 gatatgaaaa gacttcacaa gaggttcctg ttagctacgt tttgcgcgtt attcacagca
     1981 actctccagg ccgtcgatgt cactatcact gttaatggtc gggtagtcgc taaaccctgc
     2041 actattcaaa ccaaagaagc taacgttaat ctcggggatc tttatacgcg caatctgcaa
     2101 caacctggtt ctgcatctgg ctggcacaat attactttgt cattaaccga ttgtccggtt
     2161 gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg acaatacggg ttattacaaa
     2221 aatgaaggta ctgccgaaaa tattcagata gagctgaggg atgaccagga tgcgacgtta
     2281 aaaaatggcg atagcaaaac ggttattgtt gatgagatca ctcgtaatgc acagtttcca
     2341 cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc agggaacgat cgaggcgcta
     2401 atcaatgtga tctacacctg gcaataacgt cgattcgatt ttcttagcct tctttttaaa
     2461 tcaattttta accttaggat atatcatggg taaaacaatc agtataaagg tcctgttcgg
     2521 tatttatctt ctattaatgg caggcaaagt ttttgccttc agttgtaatg tcgatggcgg
     2581 gtcaagtatt ggagcaggta caacctctgt ttatgttaat ctcgaccctg taatacagcc
     2641 gggccagaat ctggttgtag acttgtctca gcatataagt tgctggaatg attacggcgg
     2701 ctggtacgac actgatcata taaacctggt acaaggctca gctttcgcag gctccctaca
     2761 gtcatataaa ggatcgctgt actggaataa tgtgacctac cccttcccgt tgacgacaaa
     2821 taccaacgta ttggatattg gtgataagac accgatgcca ttaccgttaa aactctatat
     2881 taccccagtt ggggcggccg gtggagttgt cataaaagcg ggggaagtca ttgcccgtat
     2941 tcatatgtat aaaattgcaa cattaggaag cggaaatccg cgtaatttta cgtggaatat
     3001 tatttcaaat aacaatgttg ttatgccaac aggcggctgc accgttgata gtcgtaatgt
     3061 caccgtggat ctcccggact ttcctggaag cgcagaaata ccccttggcg tatattgctc
     3121 aagcgaacaa aagctctcat tttatctttc gggtgccacc actgacagtg cccgacaagt
     3181 gtttgctaac acagcaccag atgcaacaaa agcatcggga gtgggtgtct ctctgatgcg
     3241 taatggaaaa atactggcta cgggagaaaa tgtctccttg gggacagtaa ataaaagcaa
     3301 agtacctctg ggtttaagcg caacctatgg acaaacaggg aacaaagttt cagccggaac
     3361 cgtccagtca gtcattggtg tcacttttat ctatgaataa ttgtgatttt gaccttagtc
     3421 tatgcgctcc attcacgaaa attgcgcgga taattatttt gtgaaagcta ttatcctaca
     3481 cctgtaagaa aatccgcgca caattgaagc cagagtctta ttttttatat taaaaaatat
     3541 gttaatttaa acactatgag agatgatgct taccttatcg tatttaatct attgtttaac
     3601 gataatagat taaaaataga aagagagtag tcccctactc ttatatatcc atgttggcga
     3661 taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
     3721 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgccg gtggttgggg
     3781 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat
     3841 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
     3901 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
     3961 agtcacggat aagcaggtaa acgcctcttt ctttgctgag aatacggttc aatcattact
     4021 tacctgggga gaccacgagc ttgaggctgc ggggcgactc actcagcctt tgaaatatga
     4081 tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgatg aaattggcgc
     4141 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
     4201 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
     4261 cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt
     4321 aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
     4381 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
     4441 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttactgc
     4501 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta
     4561 taacgtgcgc attggtggtg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
     4621 gcgcgatgat gctgcaagcg ccgcaggtcg gccctcattg ctggatgacg aatttattca
     4681 aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga
     4741 tatcgaacgt atttctggat taagtcagac tcaaatcgcc gaactggctg atgcatatgc
     4801 cgctgccgaa cgaaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     4861 ccagaatgta cagcaactgg tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg
     4921 tgcgggtatc tgcccactac gtggccactc taatgtacag ggcgaccgaa ccgtcggtat
     4981 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcacacc
     5041 acctcaagca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cagggcaggc
     5101 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     5161 cgctgtaccg ttaacgcaat tagatctggc ggtacacgta gccactaagc ttaaccgctc
     5221 tcatctgctg accgcacggc atagctatat tctgccggtt ctgggacgta gcgagattga
     5281 catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
     5341 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc
     5401 gggaatcgcg caggcagcac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     5461 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg attataacca
     5521 gcgcatccga catcctggtg gttttcacct gataaacgct gctgctgaaa ggcgctggat
     5581 gactcagtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     5641 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     5701 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     5761 gagtgctaaa caagctaaaa tttgccatgt aaaaaacggc gaaagagtta atcttattgc
     5821 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     5881 ccctatggct gaccgctcac tagtgaccta ttttccagaa tcgaatcaca tgctaacact
     5941 tgataaccac gatccattaa gtggcattcc tggctataaa agtattccgg ttgaattaga
     6001 accatcaaat taatgcctct tctcatttct tctgctgtca tccgcacagc agaagaatcc
     6061 ctcattgact attatcgcgc aatttgctca catggattaa attaaactac atactataag
     6121 atataaactt ctgcctacag ctgtaagaaa ctccgttcag tactgaagc
//
LOCUS       DAFAVF010000210         6149 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFAVF010000210 DAFAVF010000000
VERSION     DAFAVF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.212"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1446
                     /locus_tag="LGK42_004116"
     CDS             <1..1446
                     /locus_tag="LGK42_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK2660289.1"
                     /translation="LSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADILATEHF
                     SDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLET
                     VLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEA
                     LARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDEKLAQWIEDNVTFPSTMVD
                     RIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDV
                     LPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQ
                     GVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLAL
                     GVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLP
                     DNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1482..1685)
                     /gene="ydfZ"
                     /locus_tag="LGK42_004117"
     CDS             complement(1482..1685)
                     /gene="ydfZ"
                     /locus_tag="LGK42_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBK2660290.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1863..2549)
                     /gene="ydfH"
                     /locus_tag="LGK42_004118"
     CDS             complement(1863..2549)
                     /gene="ydfH"
                     /locus_tag="LGK42_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBK2660291.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="LGK42_004119"
     CDS             complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="LGK42_004119"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBK2660292.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3521..5566
                     /gene="dcp"
                     /locus_tag="LGK42_004120"
     CDS             3521..5566
                     /gene="dcp"
                     /locus_tag="LGK42_004120"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBK2660293.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5611..6129)
                     /locus_tag="LGK42_004121"
     CDS             complement(5611..6129)
                     /locus_tag="LGK42_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBK2660294.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1522 a         1479 c         1585 g         1563 t
ORIGIN      
        1 ttatcagcaa aagcgacact ccctgtttat gatcgtaata acctggcccc aagaattatt
       61 catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga tattcttgct
      121 acagaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg cgaacagcaa
      181 attgccgatt tacaacagca agataatctt tataccgttg cggaaatgtc ggccgatgcg
      241 tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc acgtacagat ggatggctta
      301 gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct gacaatcacc
      361 gaaaaagggt atttccactc tccggcgacc ggacagttaa tgctcgatca cccgatggtc
      421 gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa caggggtgat tgttgaggcg
      481 ctggcccgcc gtaaagcggc aggacttccc gcatttaccg tcatgtcatg tgacaacatg
      541 ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcat acgcgaaagc cgttgatgaa
      601 aaactggcac aatggatcga agataacgtg actttcccat caacaatggt ggaccgtatt
      661 gtgcccgcag tgacagagga tacgctggcg aaaatcgaac aacttaccgg tgtgggcgat
      721 cctgcgggcg ttgcctgtga acctttccgc cagtgggtaa tagaagataa ctttgttgcc
      781 ggacgtccag aatgggaaaa agcgggagcc gaactggtta gtgatgtgct gccttatgaa
      841 gagatgaagt tgcgcatgct caacggcagt cattcattcc tggcgtattt gggttatctt
      901 gctggatatc agcacattaa tgactgcatg gaagatgaac attatcgtta tgcggcgtat
      961 ggcttgatgt tgcaggaaca agcgccgacg ctgaaagtac agggcgttga tttgcaagat
     1021 tacgctaacc gattaattgc acgctatagc aacccggcgt tacgtcatcg aacctggcag
     1081 attgcgatgg atggtagcca gaaattgcca cagcggatgt tggattctgt tcgctggcat
     1141 ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg gatgcgttat
     1201 gtcggtggtg ttgatgaaca gggaaatccg atagaaatca gtgacccact gttacctgtt
     1261 attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc gcgtccagtc attgctggcg
     1321 attaaggcaa tctttggtga tgatttgcca gacaatagct tgtttactgc aaaagtgacg
     1381 gaagcgtact tgtctttatt agcgcatggc gcgaaagcga ccgtggcgaa atattccgtg
     1441 aagtaaaaaa agcaatgccg ccatcggcgg cattcacaca cttagttcat gccgagacga
     1501 atcaggtcca gaggtgccag tttctcttca caaccttcaa caactacggt ttttccgcgg
     1561 cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc agtgtgaccg
     1621 gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg atcgtaggtc
     1681 gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg gcgcaatata
     1741 aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct tttttgatgc
     1801 atgcatgagc cgtaaggcgg ggggaaaagc ctctcatccc ggatgggatg agagtaaatg
     1861 aattactctt cgctaaacca gtcgctgttt tcctggcgga tctggcgcac ggattcgctg
     1921 atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg tttttgcagg
     1981 gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac atggtcgaaa
     2041 ctcatatagc gcacgcgatc aacggtcgct ttcaggttct caatggtatc ccacgccagt
     2101 tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc aaaaaaatca
     2161 tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc cagttggtag
     2221 cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc aatggcctga
     2281 cggataaaac tgccgtttcg cacctgggcc atggaaattt tgttgacgta gctgccacgc
     2341 tgcggacgaa tttgaatcag gccgttttcc gccagtttaa taaaggcttc acgaaccggc
     2401 tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt gcctggtgca
     2461 atcaggcaat ggacaatgtc gcgacgaaga atacgataaa tctgctgatt gacaggctgt
     2521 gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag ttaactgcat
     2581 gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt ataaaaatta
     2641 ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt ccagggtatt
     2701 gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga catcttctgg
     2761 cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg
     2821 gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga cgcgcaccgc
     2881 cgtaccatgc aggtccgtac gcagattcag gctaaactga cgaacaaacg ctttcgtcgc
     2941 accgtaaacg ttaccaccgg cataaggcca gctacctgcc gttgagccaa tgttaataat
     3001 atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat ataccaggcc
     3061 tttattgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat gcgcaggctc
     3121 catgcccaac gccaggccgg cattatttac caggatatca atattgctcc actcggcagg
     3181 gagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt gggcgatata
     3241 cagattatct cccagttcgt cttttaactc ctgcaaccgt tcctgacgac ggccagtggc
     3301 gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa aacctgccgt
     3361 tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt gtcaataaca
     3421 tagcacggga tgcttaagag cgggtaatac tggattgcaa gattgcactc cgttatcagt
     3481 aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt
     3541 ccttgtgcaa agcacactgc cgtatctggc tcctcatttt gatcaaattg ccaatcatca
     3601 ctatcgcccg gcattcgatg agggaatgca gcaaaagcgg gcagaaattg ctgccatcgc
     3661 gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac aaagcggaga
     3721 attacttacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata ccaatgatga
     3781 attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta atgatatcta
     3841 tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg aatccctggg
     3901 gcttgatagt gaatccatcc gcctggtgga ggtgattcat caacgttttg tccttgccgg
     3961 agccaaactt gcgcaagctg ataaagcaaa attaaaagta ctgaatacag aagctgcgac
     4021 actgaccagt cagtttaacc agcggttact ggcagcaaat aaatccggcg gtctggttgt
     4081 gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg cggcagaggc
     4141 ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata ccacccaaca
     4201 gccggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg
     4261 gacgcgagcg gaaaaaaatg atggcaatga tacccgcgct atcattcaac gtctggtgga
     4321 gatccgtgca caacaggcga cattacttgg ttttcctcat tatgccgcat ggaaaatcgc
     4381 cgatcagatg gcaaaaacac ctgaagcagc gctcaacttt atgcgggaaa ttgttccagc
     4441 ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttattg ataagcagca
     4501 gggcgggttt agcgcgcagc cgtgggactg ggcattttat gccgaacagg tacggcggga
     4561 gaaatttgat cttgatgagg cgcagctcaa gccatatttt gaattaaaca cggtgttgaa
     4621 tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg aacgttttga
     4681 tattcctgtc taccatcctg acgttcgtgt gtgggaaatt tttgatcata atggcgtggg
     4741 actggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg gtgcatggat
     4801 gggcaatttt gttgagcaat caacgcttaa tgaaacgcat ccggtaattt ataacgtttg
     4861 caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg atgtcataac
     4921 cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc gttatgccac
     4981 gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca
     5041 ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga gcggggcagc
     5101 aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca aagggtatga
     5161 gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa
     5221 cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg aaaatatgga
     5281 tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt ttggtggcgg
     5341 atatgccgca ggttattacg cttatctgtg gacacaaatg ttggccgatg atggttatca
     5401 gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg ctgcgttttc gcgaggcgat
     5461 cctttccaga ggtaacagcg aagatctgga acgcctgtat cgacaatggc gcggtaaggc
     5521 tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac caccaatggc
     5581 tgtcctggag atatcgccag gccagcctgg ttatagcagt aattgcagca gctgggcgag
     5641 ggcaaacctc accgctaaag ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc
     5701 agtataggtc tggcctttaa tatgccacgc gaaccagacg gtacccgctg gcgtaccatc
     5761 ttcaccgccc tccggtccgc cgtagccggt aatggcaata ctgacatcag catccgcacg
     5821 ctctatggca ccggttgcca tttctgctgc cactttctca ctcaccgcag aatatcgttc
     5881 aagagattgc tggcttacgc tgaggatttt catctttgcc tgatcggtga aagtaacaaa
     5941 gcctgcaccg taaaatttgg gtgtatcttc agctgcacac agggcgctag ccaacttacc
     6001 gccggtgcat gattcggcgg tagttaagcg taattgatcc gcgataagtc tttgactcaa
     6061 cgcggatgtc aggttttcaa tagtgtcggt atctgcaagt tgtacaattt tatcgctatt
     6121 aatgttcact gttttttgtc cacgctttt
//
LOCUS       DAFAVF010000211         6094 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFAVF010000211 DAFAVF010000000
VERSION     DAFAVF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.213"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..295
                     /locus_tag="LGK42_004122"
     CDS             <1..295
                     /locus_tag="LGK42_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBK2660295.1"
                     /translation="GNSDPAHTATAPGGLSAKAPAMTPLMLDTSSRKLVAWDGTTDGA
                     AVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAFAGTAISIV"
     gene            351..1376
                     /locus_tag="LGK42_004123"
     CDS             351..1376
                     /locus_tag="LGK42_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBK2660296.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            1418..1816
                     /locus_tag="LGK42_004124"
     CDS             1418..1816
                     /locus_tag="LGK42_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBK2660297.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1828..2181
                     /locus_tag="LGK42_004125"
     CDS             1828..2181
                     /locus_tag="LGK42_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBK2660298.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            2193..2771
                     /locus_tag="LGK42_004126"
     CDS             2193..2771
                     /locus_tag="LGK42_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660299.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2768..3163
                     /locus_tag="LGK42_004127"
     CDS             2768..3163
                     /locus_tag="LGK42_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660300.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            3171..3911
                     /locus_tag="LGK42_004128"
     CDS             3171..3911
                     /locus_tag="LGK42_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660301.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            3927..4349
                     /locus_tag="LGK42_004129"
     CDS             3927..4349
                     /locus_tag="LGK42_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBK2660302.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSVGKCSTVS"
     gene            4331..4765
                     /locus_tag="LGK42_004130"
     CDS             4331..4765
                     /locus_tag="LGK42_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBK2660303.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4758..>6094
                     /locus_tag="LGK42_004131"
     CDS             4758..>6094
                     /locus_tag="LGK42_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2660304.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTTEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKK"
BASE COUNT         1372 a         1553 c         1927 g         1242 t
ORIGIN      
        1 gggcaacagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg cgaaagcgcc
       61 tgcaatgacc ccgctgatgc tggacacctc cagccgtaag ctggttgcgt gggatggcac
      121 caccgacggt gctgccgttg gcattcttgc ggttgctgct gaccagacca gcaccacgct
      181 gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg aggctgccag
      241 cgacgagacg aaaaaacgga ccgcgtttgc cggaacggca atcagcatcg tttaacttta
      301 cccttcatca ctaaaggccg cctgtgcggc tttttttacg ggattttttt atgtcgatgt
      361 acacaaccgc ccaactgctg gcggcaaatg agcagaaatt taagtttgat ccgctgtttc
      421 tgcgtctctt tttccgtgag agctatccct tcaccacgga gaaagtctat ctctcacaaa
      481 ttccgggact ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt gaggttatcc
      541 gttcccgtgg cggctccacc tctgaattta cgccgggata tgtcaagccg aagcatgaag
      601 tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga tccgcagaat ctggcggacc
      661 cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg tgacgaagag ctggctattg
      721 ctcaggtcga agagatgcag gcagtttctg ccgtgcttaa gggcaaatac accatgaccg
      781 gtgaagcctt cgatccggtt gaggtggata tgggccgcag tgaggagaat aacatcacgc
      841 agtccggcgg cacggagtgg agcaagcgtg acaagtccac gtatgacccg accgacgata
      901 tcgaagccta cgcgctgaac gccagcggtg tggtgaatat catcgtgttc gatccgaaag
      961 gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa gctggatacc cgtcgtggct
     1021 ctaattccga gctggagaca gcggtgaaag acctgggtaa agcggtgtcc tataagggga
     1081 tgtatggcga tgtggccatc gtcgtgtatt ccggacagta cgtggaaaac ggcgtcaaaa
     1141 agaacttcct gccggacaac acgatggtgc tggggaacac tcaggcacgc ggtctgcgca
     1201 cctatggctg cattcaggat gcggacgcac agcgcgaagg cattaacgcc tctgcccgtt
     1261 acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga gttcaccatg attcagtcag
     1321 caccgctgat gctgctggct gaccctgatg agttcgtgtc cgtacaactg gcgtaatcat
     1381 ggcccttcgg ggccattgtt tctctgtgga ggagtccatg acgaaagatg aactgattgc
     1441 ccgtctccgc tcgctgggtg aacaactgaa ccgtgatgtc agcctgacgg ggacgaaaga
     1501 agaactggcg ctccgtgtgg cagagctgga agaggagctt gatgacacgg atgaaactgc
     1561 cggtcaggac acccctctca gccgggaaaa tgtgctgacc ggacatgaaa atgaggtggg
     1621 atcagcgcag ccggataccg tgattctgga tacgtctgaa ctggtcacgg tcgtggcact
     1681 ggtgaagctg catactgatg cacttcacgc cacgcgggat gaacctgtgg catttgtgct
     1741 gccgggaacg gcgtttcgtg tctctgccgg tgtggcagcc gaaatgacag agcgcggcct
     1801 ggccagaatg caataacggg aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc
     1861 attgcccgcg ccgatgaaac gatacgcggg tacatgggaa cgtcagccac cattacatcc
     1921 ggtgagcagt caggtgcggt gatacgtggt gtttttgatg accctgaaaa tatcagctat
     1981 gccggacagg gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg gactgatgag
     2041 gtgcggcagc tgcggcgtgg agacacgctg accatcggtg aggaaaattt ctgggtagat
     2101 cgggtttcgc cggatgatgg cggaagttgt catctctggc ttggacgggg cgtaccgcct
     2161 gccgttaacc gtcgccgctg aaagggggat gtatggccat aaaaggtctt gagcaggccg
     2221 ttgaaaacct cagccgtatc agcaaaacgg cggtgcctgg tgccgccgca atggccatta
     2281 accgcgttgc ttcatccgcg atatcgcagt cggcgtcaca ggttgcccgt gagacaaagg
     2341 tacgccggaa actggtaaag gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc
     2401 aggccagaat caaagttaac cggggggatt tgcccgtaat caagctgggt aatgcgcggg
     2461 ttgtcctttc gcgccgcagg cgtcgtaaaa aggggcagcg ttcatccctg aaaggtggcg
     2521 gcagcgtgct tgtggtgggt aaccgtcgta ttcccggcgc gtttattcag caactgaaaa
     2581 atggccggtg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg
     2641 tgaaaatccc gatggcggtg ccgctgacca cggcgtttaa acaaaatatt gagcggatac
     2701 ggcgtgaacg tcttccgaaa gagctgggct atgcgctgca gcatcaactg aggatggtaa
     2761 taaagcgatg aaacatactg aactccgtgc agccgtactg gatgcactgg agaagcatga
     2821 caccggggcg acgttttttg atggtcgccc cgctgttttt gatgaggcgg attttccggc
     2881 agttgccgtt tatctcaccg gcgctgaata cacgggcgaa gagctggaca gcgatacctg
     2941 gcaggcggag ctgcatatcg aagttttcct gcctgctcag gtgccggatt cagagctgga
     3001 tgcgtggatg gagtcccgga tttatccggt gatgagcgat atcccggcac tgtcagattt
     3061 gatcaccagt atggtggcca gcggctatga ctaccggcgc gacgatgatg cgggcctgtg
     3121 gagttcagcc gatctgactt atgtcattac ctatgaaatg tgaggacgct atgcctgtac
     3181 caaatcctac aatgccggtg aaaggtgccg ggaccaccct gtgggtttat aaggggagcg
     3241 gtgaccctta tgcgaatccg ctttcagacg ttgactggtc gcgtctggca aaagttaaag
     3301 acctgacgcc cggcgaactg accgctgagt cctatgacga cagctatctc gatgatgaag
     3361 atgcagactg gactgcgacc gggcaggggc agaaatctgc cggagatacc agcttcacgc
     3421 tggcgtggat gcccggagag caggggcagc aggcgctgct ggcgtggttt aatgaaggcg
     3481 atacccgtgc ctataaaatc cgcttcccga acggcacggt cgatgtgttc cgtggctggg
     3541 tcagcagtat cggtaaggcg gtgacggcga aggaagtgat cacccgcacg gtgaaagtca
     3601 ccaatgtggg acgtccgtcg atggcagaag atcgcagcac ggtaacagcg gcaaccggca
     3661 tgaccgtgac gcctgccagc acctcggtgg tgaaagggca gagcaccacg ctgaccgtgg
     3721 ccttccagcc ggagggcgta accgacaaga gctttcgtgc ggtgtctgcg gataaaacaa
     3781 aagccaccgt gtcggtcagt ggtatgacca tcaccgtgaa cggcgttgct gcaggcaagg
     3841 tcaacattcc ggttgtatcc ggtaatggtg agtttgctgc ggttgcagaa attaccgtca
     3901 ccgccagtta atccggagag tcagcgatgt tcctgaaaac cgaatcattt gaacataacg
     3961 gtgtgaccgt cacgctttct gaactgtcag ccctgcagcg cattgagcat ctcgccctga
     4021 tgaaacggca ggcagaacag gcggagtcag acagcaaccg gaagtttact gtggaagacg
     4081 ccatcagaac cggcgcgttt ctggtggcga tgtccctgtg gcataaccat ccgcagaaga
     4141 cgcagatgcc gtccatgaat gaagccgtta aacagattga gcaggaagtg cttaccacct
     4201 ggcccacgga ggcaatttct catgctgaaa acgtggtgta ccggctgtct ggtatgtatg
     4261 agtttgtggt gaataatgcc cctgaacaga cagaggacgc cgggcccgca gagcctgttt
     4321 ctgtgggaaa gtgttcgacg gtgagctgag ttttgccctg aaactggcgc gtgagatggg
     4381 gcgacccgac tggcgtgcca tgcttgccgg gatgtcatcc acggagtatg ccgactggca
     4441 ccgcttttac agtacccatt attttcatga tgttctgctg gatatgcact tttccgggct
     4501 gacgtacacc gtgctcagcc tgtttttcag cgatccggat atgcatccgc tggatttcag
     4561 tctgctgaac cggcgcgagg ctgacgaaga gcctgaagat gatgtgctga tgcagaaagc
     4621 ggcagggctt gccggaggtg tccgctttgg cccggacggg aatgaagtta tccccgcttc
     4681 cccggatgtg gcggacatga cggaggatga cgtaatgctg atgacagtat cagaagggat
     4741 cgcaggagga gtccggtatg gctgaaccgg taggcgatct ggtcgttgat ttgagtctgg
     4801 atgcggccag atttgacgag cagatggcca gagtcaggcg tcatttttcc ggtacggaaa
     4861 gtgatgcgaa aaaaacagcg gcagtcgttg aacagtcgct gagccgacag gcgctggctg
     4921 cacagaaagc ggggatttcc gtcgggcagt ataaagccgc catgcgtatg ctgcctgcac
     4981 agttcaccga cgtggccacg cagcttgcag gcgggcaaag tccgtggctg atcctgctgc
     5041 aacagggggg gcaggtgaag gactccttcg gcgggatgat ccccatgttc agggggcttg
     5101 ccggtgcgat caccctgccg atggtggggg ccacctcgct ggcggtggcg accggtgcgc
     5161 tggcgtatgc ctggtatcag ggcaactcaa ccctgtccga tttcaacaaa acgctggtcc
     5221 tttccggcaa tcaggcggga ctgacggcag atcgtatgct ggtcctgtcc agagccgggc
     5281 aggcggcagg gctgacgttt aaccagacca gcgagtcact cagcgcactg gttaaggcgg
     5341 gggtaagcgg tgaggctcag attgcgtcca tcagccagag tgtggcgcgt ttctcctctg
     5401 catccggcgt ggaggtggac aaggtcgctg aagccttcgg gaagctgacc acagacccga
     5461 cgtcggggct gacggcgatg gcacgccagt tccataacgt gacgacggag cagattgcgt
     5521 atgttgctca gttgcagcgt tccggcgatg aagccggggc attgcaggcg gcgaacgagg
     5581 ccgcaacgaa agggtttgat gaccagaccc gccgcctgaa agagaacatg ggcacgctgg
     5641 agacctgggc agacaggact gcgcgggcat tcaaatccat gtgggatgcg gtgctggata
     5701 ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg tttaagaaag
     5761 cagacgacat ctggaatctg cgcaaggatg attattttgt taacgatgaa gcgcgggcgc
     5821 gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga aagaaggctg
     5881 agcagcagac tcaacaggac aaaaatgcgc agcagcagag cgataccgaa gcgtcacggc
     5941 tgaaatatac cgaagaggcg cagaaggctt acgaacggct gcagacgccg ctggagaaat
     6001 ataccgcccg tcaggaagaa ctgaacaagg cactgaaaga cgggaaaatc ctgcaggcgg
     6061 attacaacac gctgatggcg gcggcgaaaa agga
//
LOCUS       DAFAVF010000212         6064 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFAVF010000212 DAFAVF010000000
VERSION     DAFAVF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.214"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..947
                     /locus_tag="LGK42_004132"
     CDS             <1..947
                     /locus_tag="LGK42_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBK2660305.1"
                     /translation="SKCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRI
                     WSIFWVLALWGVVQWLALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLW
                     YLYALIVYFVVCKIFSRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAW
                     FGATLMTCVKEVPLRRHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQ
                     FGMRSTSLLNVIGSNTIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFV
                     SLFICTVAGLLVRKLSQRAFSDLLFSPPSLPAAVSYSR"
     gene            1075..4521
                     /gene="mfd"
                     /locus_tag="LGK42_004133"
     CDS             1075..4521
                     /gene="mfd"
                     /locus_tag="LGK42_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK2660306.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKSGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            4665..5627
                     /gene="ldtC"
                     /locus_tag="LGK42_004134"
     CDS             4665..5627
                     /gene="ldtC"
                     /locus_tag="LGK42_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBK2660307.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(5710..5967)
                     /gene="bhsA"
                     /locus_tag="LGK42_004135"
     CDS             complement(5710..5967)
                     /gene="bhsA"
                     /locus_tag="LGK42_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBK2660308.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         1392 a         1561 c         1631 g         1480 t
ORIGIN      
        1 tgagcaaatg ctggatctat ttcaatcttt accttgcacc ctttcgtatg ccggtttttt
       61 tctttatttc cggctatttg attcgccgct atatcgacag cgtaccgtgg ggaaattgtc
      121 tcgataaacg catctggagc atcttttggg tgctggcgct ttggggcgtg gtgcagtggc
      181 tggcgttaag tgcgcttaac cagtggctgg cacctgagcg cgatttaagc aacgcttcca
      241 atgccgcgta tgccgattcc accggtgagt tcctgcacgg gatgatcaca gccagcacca
      301 gcttgtggta tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt tttagccgtc
      361 tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca
      421 cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc ttattacagc cttggcgcat
      481 ggtttggcgc aacattaatg acctgtgtta aagaggtgcc gttgcgccgc catctgctga
      541 tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt
      601 tgtcgctggt atcgattgtg gtgatcatga agctgtttta tcaatacgag caacaattcg
      661 gtatgcgctc caccagcctg ctgaatgtga ttggctccaa caccattgct atctacacca
      721 cccatcgcat tctggttgaa atattcagct taactctgct tgcgcaaatg aacgcagcac
      781 gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta tccctttgtc agtttgttca
      841 tctgtactgt tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc agcgatctgt
      901 tgttctcccc gccttcactg cccgctgccg tcagttactc ccgctaagcc atcgccccag
      961 ccggggcgat tcaatttgct aaaccatgtc gattacggat aatgaacagc attgcttatc
     1021 agagatatgc ccccatatgt tgaggcatat cctaacgaga atctgacaac cgttatgcct
     1081 gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta
     1141 accggcgcag cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc cggtccggtg
     1201 gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat cagccagttc
     1261 accgatcaga tggtgatgaa tctggcggac tgggaaactc ttccctacga cagtttttcg
     1321 cctcatcagg acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag
     1381 cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc acacagtttt
     1441 ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga
     1501 acccaactgg acagcgccgg ttatcgccat gttgaccagg tgatggagca cggcgaatac
     1561 gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt
     1621 gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg
     1681 ctggaggaag tagaagcgat caatctgctg cccgcgcacg aatttccgac cgataaagcg
     1741 gcaattgaac tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccggaa
     1801 catatttacc agcaagtgag taaaggcaca ttacctgccg ggatcgagta ctggcagcca
     1861 ttgttcttca gcgaaccact gccgccgctg ttcagttatt tccctgccaa taccttgctc
     1921 gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc aggctgacac gctggcgcgt
     1981 tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc gctctggctg
     2041 cgggtggacg agctcttctc agagctgaaa aactggcccc gggtgcagct aaaaactgaa
     2101 catttaccga caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc
     2161 gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta agttcctcga gactttcgac
     2221 ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc
     2281 gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg
     2341 cgttatctga tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg taatctggcg
     2401 ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca ggattctcgc
     2461 cgcaccatca accccgatac actgatccgt aaccttgcgg agctgcatat tggtcagccg
     2521 gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa tgaccacgct cgaagcgggc
     2581 ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact gtatgttccg
     2641 gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg cggaagaaaa cgccccgctg
     2701 cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt
     2761 gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg
     2821 tttaaacacg atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg
     2881 ccggatcagg cgcaggccat taatgcggta cttagcgaca tgtgtcagcc gctggcaatg
     2941 gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat gcgcgccgct
     3001 ttcctggcag tagataacca caagcaggtg gcggtgttgg tgcctaccac ccttctcgcg
     3061 cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg
     3121 atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg
     3181 aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa gtttaaagat
     3241 ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgtatt
     3301 aaagcgatgc gcgcgaacgt ggatattctg acgcttactg caacgccgat cccacgtacg
     3361 ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt
     3421 cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg ggaggcgatc
     3481 ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct acaatgatgt ggaaaacatt
     3541 cagaaagccg ccgaacggct ggcagaactg gtgccagaag cgcggatcgc catcggtcac
     3601 gggcagatgc gcgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc
     3661 aacgtgctgg tttgtaccac cattatcgaa accgggatcg acatcccgac agccaacacc
     3721 attatcattg aacgtgcgga tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc
     3781 gtcggacgtt cgcatcatca ggcatatgca tggttgctga caccgcatcc aaaagcgatg
     3841 actaccgatg cacaaaaacg tcttgaagcg attgcctcgc tggaagatct cggggcaggt
     3901 tttgcgctgg caacgcacga tctggagatt cgcggcgcgg gtgaactgct tggcgaagaa
     3961 caaagtggct caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc
     4021 gtcgatgcac tgaaatccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca
     4081 gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcattcctga cgtgaatacg
     4141 cgtttgtcgt tctataaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc
     4201 aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac cctgctggat
     4261 gttgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag
     4321 aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt
     4381 ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgcct gaaatttatt
     4441 caggatttga gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg
     4501 gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc atctgcaaca
     4561 tttacaaatt ctttgcactt ccctgcacta tccgacactg tcaccatcca taattcaggc
     4621 ttatctgttt attacaataa ccttatattt attatggatt tttggtgatg atcaaaacgc
     4681 gtttttctcg ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg gcgctaccgg
     4741 caaaagccaa cacctggccg ctgccgccag cgggcagtcg tctggttggc gaaaacaaat
     4801 ttcatgtggt ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa tataacgtcg
     4861 gctttctcgc tctgttacag gctaaccctg gcgttgatcc ttacgtaccg cgcgcgggta
     4921 gcgtgttaac gatcccgttg caaaccctac ttccagatgc gccgcgcgaa ggcattgtga
     4981 tcaatattgc ggagctgcgt ctctattact acccgccggg taaaaattcg gtaaccgtgt
     5041 atccaatagg tattggtcag ttaggtggtg acacgctgac accgacaatg gtgaccaccg
     5101 tttcagacaa acgtgcaaac ccaacctgga cgccaacggc aaacatccgc gcccgttata
     5161 aagcacaggg aattgagttg cctgcggtag tgccggctgg accggataac ccaatgggcc
     5221 atcatgcgat tcgtctggcg gcctatggcg gcgtttattt gcttcacggt acgaacgccg
     5281 atttcggcat tggcatgcgg gtaagttctg gctgtattcg tctgcgggat gacgatatca
     5341 aaacactctt tagccaggtc accccaggca ccaaagtgaa tatcatcaac actccgataa
     5401 aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga
     5461 agattgatga cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg caatcattta
     5521 aagatgcagc acaaactgac gctgaagtga tgcaacatgt gatggatgtc cgttccggga
     5581 tgccggtgga tgtccgccgt catcaagtga gcccacaaac tctgtaaatc ctggataaaa
     5641 aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag
     5701 ggttaatgct tatttataaa ttactgctgt tccatggagg gtattcggac cggttacaga
     5761 agtaatacgg aaagattttg cgcccatctc atccgctttt tgcgccagct gctcttccag
     5821 cgatcccaga tttgtccccg cgttagcact gattgtaccg actttttgtt ggccttctgg
     5881 cgttgactga acttcgacag ccgcaaagct ggcaaatgac atggagctta aaatcgccgc
     5941 agcgatgagg gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
     6001 agcaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
     6061 tttt
//
LOCUS       DAFAVF010000213         5926 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFAVF010000213 DAFAVF010000000
VERSION     DAFAVF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.215"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            28..264
                     /gene="traQ"
                     /locus_tag="LGK42_004136"
     CDS             28..264
                     /gene="traQ"
                     /locus_tag="LGK42_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBK2660309.1"
                     /translation="MWVFSLGVWFHIVARLVYSKPWMAFFLAELIAAILVLFGAYQVL
                     DAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            251..796
                     /gene="trbB"
                     /locus_tag="LGK42_004137"
     CDS             251..796
                     /gene="trbB"
                     /locus_tag="LGK42_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBK2660310.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FSYTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARVDTVLQMYGGKKGAK"
     gene            726..1067
                     /gene="trbJ"
                     /locus_tag="LGK42_004138"
     CDS             726..1067
                     /gene="trbJ"
                     /locus_tag="LGK42_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBK2660311.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESS
                     EISEGTYNERK"
     gene            1021..1434
                     /gene="trbF"
                     /locus_tag="LGK42_004139"
     CDS             1021..1434
                     /gene="trbF"
                     /locus_tag="LGK42_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBK2660312.1"
                     /translation="MNHRKYQRELIMRENKSNPELKIRSTERDYKYISRITGRYAGLS
                     LVFLTAGIVLWTVMDIIFDACIDSWKADPELNNSSYMWNILIYAIPYALYALAAGFLV
                     TFFSVPNVRINIRKYRDIPAEMSYAPGEHIKGGQE"
     gene            1434..2807
                     /locus_tag="LGK42_004140"
     CDS             1434..2807
                     /locus_tag="LGK42_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBK2660313.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTR"
     gene            2804..5626
                     /gene="traG"
                     /locus_tag="LGK42_004141"
     CDS             2804..5626
                     /gene="traG"
                     /locus_tag="LGK42_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBK2660314.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPLLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAVAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            5648..>5926
                     /locus_tag="LGK42_004142"
     CDS             5648..>5926
                     /locus_tag="LGK42_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HBK2660315.1"
                     /translation="MRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLFIW
                     LFSQIRKKRDVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYEL"
BASE COUNT         1490 a         1363 c         1616 g         1457 t
ORIGIN      
        1 tctttacccc ggcttgatat tacgggaatg tgggtatttt ccctgggtgt ctggtttcat
       61 atcgtcgccc gtcttgttta cagcaaaccc tggatggctt tttttctggc ggagctgatt
      121 gcggccattc tcgtactgtt cggcgcatac caggtgctgg atgcatggat tgcccgtgtc
      181 agccgggaag agcgtgaggc actggaagcc aggcaacagg ccatgatgga agggcagcag
      241 gagggcggac atgtctctca ctaaatcact gctgttcacc ctgttgctga gcgccgctgc
      301 tgtgcaggcc tccacccggg atgaaataga gcgactctgg aatccgcagg gtatggctac
      361 gcagcctgca caaccggcag caggcacgtc agccaggaca gcaaagccgg ctccccgctg
      421 gttccgcctc agtaatggca ggcaggttaa cctggccgac tggaaggtgg tgctgtttat
      481 gcaggggcat tgcccttact gtcaccagtt tgacccggta ctgaaacagc tggcacagca
      541 gtacggcttt tccgtttttt cctacaccct ggatggtcag ggggatacgg cctttcctga
      601 agcattaccg gtgccaccgg aggtgatgca gaccttcttc ccgaatatcc cggtggccac
      661 accgaccacc ttcctggtca acgtcaacac gcttgaggca ttaccgcttt tacagggggc
      721 aacggatgcc gccggtttta tggcgcgggt ggataccgtt ttgcagatgt acggaggaaa
      781 aaaaggtgcg aaataagcag gtggtgttac tcattgccgg aatatccggc atcgtgacgg
      841 gaataatcgt cagcctgaat atccccttta tccgtcaggg gcttttttat cccgccagcc
      901 ctgtagaaat tgtcgtttcg ctgagtctta ccttttctgt ttctgttgtg ttttttgtgg
      961 gggcaattgt gggatggatt tcggtgtctg aaatatatta cagccgcatg accggtctga
     1021 atgaatcatc ggaaatatca gagggaactt ataatgagag aaaataaaag taatcctgag
     1081 ctcaaaatca gaagtacaga acgagattat aaatacattt ccagaattac tggcaggtat
     1141 gctgggttgt ctctggtatt cctgactgcc ggaattgttt tatggacagt tatggatatt
     1201 atttttgatg cctgtattga ttcgtggaaa gcggacccgg aattaaataa ttcgagttat
     1261 atgtggaata tcctgatata tgccattccg tatgcgttat atgcgcttgc agccgggttt
     1321 cttgttacat ttttttctgt ccccaatgta cgaattaata tacggaaata tcgagatatt
     1381 cctgctgaaa tgtcgtatgc accgggagaa cacattaagg gagggcaaga atgatgccac
     1441 gcattaaacc tcttctggtt ctttgtgccg ccttactgac ggtgacgcct gcagcgtcag
     1501 cggatgtgaa cagcgacatg aatcagttct ttaataagct gggatttgct tccaatacca
     1561 cacagccggg cgtctggcag gggcaggccg ccggctatgc ttacggtggc tccctgtatg
     1621 cccgtactca ggtcaaaaat gttcagctaa tttccatgac gctgccggat atcaatgctg
     1681 gatgtggcgg catcgacgcc tatctcggct cgttcagttt tattaatggt gagcagttgc
     1741 agcgttttgt taagcagatt atgagcaatg ctgccggtta cttttttgac cttgccctgc
     1801 agacaacggt gccggaaata aaaaccgcaa aagacttcgt gcagaaaatg gcaagcgaca
     1861 ttaacagtat gaacctcagt tcctgccagg ctgcacaggg gattatcggc gggcttttcc
     1921 cccggacgca ggtgtcccag cagaaagtct gtcaggacat tgccggtgag agcaatattt
     1981 ttgctgactg ggcggcttcc cggcagggat gtaccgttgg cgggaaatct gacagtgtca
     2041 gggataaagc cagcgacaag gataaggagc gggtgaccaa aaacatcaac atcatgtgga
     2101 atgcgctttc caaaaacaga atgtttgacg gcaacaaaga gctgaaagaa tttgtgatga
     2161 cgctgaccgg ctcactggtg tttggtccca acggcgaaat cacaccgctg tcagccagaa
     2221 ccactgaccg ctcaattatc cgggccatga tggaaggcgg cacagcaaaa atttatcact
     2281 gcaacgattc tgataaatgc ctgaaagtgg tggcagacac accggtgact atcagccggg
     2341 ataatgcact gaagtctcag attactaaac ttctggccag cattcagaac aaggcggtca
     2401 gtgacacgcc tctggatgac aaggaaaaag gctttatttc cagtaccacc atccccgtct
     2461 tcaaatacct ggttgacccg cagatgctcg gagtttccaa cagcatgatt taccagctga
     2521 cggactatat cggttacgac atcctgctgc agtacattca ggagctgata cagcaggccc
     2581 gggcgatggt ggccacggga aattatgacg aagcggttat cggacatatt aacgacaaca
     2641 tgaatgatgc cacccggcag attgcggcgt ttcagtcaca ggtgcaggta cagcaggatg
     2701 cgctgctggt tgtcgatcgt cagatgagct acatgcgtca gcagctttcc gcccgcatgc
     2761 tcagtcgtta ccaaaacaac tatcacttcg gagggagcac gcggtgaatg aagtttatgt
     2821 gattgccggt ggtgagtggt tgcggaataa cctgaacgcc attgccgcct ttatgggaac
     2881 caggacgtgg gattccattg aaaaaattgc gctcacattg tctgttctcg cggtggccgt
     2941 aatgtgggta cagcggcaca acgtgatgga tttgctgggc tgggtggccg tatttgtgct
     3001 tatcagcctg ctggttaatg tccgcacatc ggtgcagatt attgataaca gtgacctggt
     3061 caaagttcac cgggtggata atgtgccggt cggactggcg atgccacttt cactgacgac
     3121 ccgtatcggg catgcaatgg tggccagtta cgagatgatt ttcacgcaac cggacagtgt
     3181 cacctacagc aaaacgggga tgctgtttgg cgcagaactg gtatcaaaaa gcaccgactt
     3241 tttgtcccgc aatccggaaa tcgccaatct tttccaggat tatgtgcaga actgtgttat
     3301 gggggatatt tacctgaacc acaaatacac gctggaggag ctgatggcct ccgctgatcc
     3361 ctacacgctg attttttccc gccccagccc gctgcggggc gtttatgaca gcaataataa
     3421 tttcgtaacc tgtaaggacg cgtcggtttc gctgaaagac aagctgaatc tggacacaca
     3481 gagtggcgga aaaacctggc attattatgc ccagcagcta tttggtggac gccctgatcc
     3541 taacctgctg ttcagtacgc tgattggcga cagttacagt tacttctatg gctcaagtaa
     3601 atcagccagc cagatcatcc gccagaatgt caccatcaat gcgctgaagg aggggatcac
     3661 cagttatgcc gcacgtaatg gtgattcggc cagcctggtg aatctggcca ccacgtcatc
     3721 gatggagaaa caacgtctgg cacatgtctc catcggtcat gtggcgatgc ggacactgcc
     3781 catgacgcag accatcctga cggggatcgc catcggtatt ttcccgctcc tggtactggc
     3841 ggctgtattc aacaaactga cgctgtctgt cctgaaaggt tatgtgtttg ccctgatgtg
     3901 gctgcagtca tggccactct tgtacgccat tctgaacagt gccatgacat tttatgcgaa
     3961 acagaatggt gcgccggtgg tgctctctga aatttcacag atacaactga aatactccga
     4021 tctggcttcc actgccgggt atctttccat gatgatcccg ccattatcct ggatgatggt
     4081 cagggggctt ggggccggat tttccagtgt gtacagccat ttcgcttcct ctgctatcag
     4141 cccgactgcc agtgcggcag gtagtgtggt tgacggtaat tactcctacg gcaacatgca
     4201 gacggaaaac gtgaacggct tcagctggag caccaacagc accacgtcgt ttggtcagat
     4261 gatgtaccag accggcagtg gcgcaaccgc cacacagacc cgtgacggta atatggtgat
     4321 ggatgcaagc ggagcgatgt cccgtttacc ggtcggtatc aatgcaacgc gtcagattgc
     4381 agtggcacaa caggaaatgg cccgggaggc gtcgaacaga gcagaaagtg ccctgcatgg
     4441 tttcagcagc agtattgcca gtgcctggaa cacgctcagt cagtttggtt ctaaccgggg
     4501 gagcagtgac tctgtcacca gtggtgctga cagtacgatg agtgcacagg actccatgat
     4561 ggccagccgt atgcgcagtg cagtggaaag ctatgcgaag gcgcataata tcagtaatga
     4621 gcaggcgact caggaactgg catccagaag tacgagagct tctgccggga tgtatggaga
     4681 tgctcatgct gaatgggggg ttaaacctaa aattcttggt gttggtggag gattaggcgt
     4741 tagaggtggt ggtcgggctg gcattgactg gagtgatgat gatgcgcatc aggccagcag
     4801 tggttcacgg gccagctatg atgctcgtca tgatattgac gctaaagcca gtaaggactt
     4861 taaagaggcc agcgattact ttaccagtcg caaagtcagt gaatccggca gccatactga
     4921 caataatgct gattcccggg tggatcaact gtctgcagcc ctgaattcag cgaaacagag
     4981 ttacgaccag tacacgacga atatgacccg cagccatgaa tatgctgaaa tggcatcccg
     5041 tactgaaagc atgagtgggc agatgagcga agatttgtcg caacagtttg cacagtatgt
     5101 gatgaaacac gcaccgcagg atgccgaagc tattctgaca aataccagtt cgccggagat
     5161 tgcagaacgc cgtcgggcaa tggcctggtc ttttgtgcag gaacagatac agcctggtgt
     5221 tgataatgcc tggcgggaat cccgtggcga tattggtaaa ggaatggaga gcgtaccttc
     5281 gggtggaggc agtcaggata ttattgctga ccatcaggga catcaggcca ttattgagca
     5341 aagaacgcag gacagtaata tccgtaatga tgtaaaacat caggttgata atatggtcac
     5401 agaatataaa gggaatatag gtgatactca gaacagcata cgtggcgagg aaaatattgt
     5461 aagaggacag tattctgaac tgcagaatca ccataaaaca gaggcattat ctcagaataa
     5521 taaatataat gaagaaaagt cggctcagga aagaatgccc ggggctgaca gtccacagga
     5581 actgatgaaa agggcaaagg agtatcagga taagtataag caataatact gtgtttggga
     5641 gatgataatg agatgtttag cccatattgc tctggtaaca gtgatacagt ttatcgcctg
     5701 ttatctggct gattggggga atgccgaaac agtttttatg ctgttcttca tagtgctatg
     5761 gcagggactg tttatctggt tgttctctca aatacgaaaa aaacgggatg tgtcggatga
     5821 gtttaagttt agtaaaggcg tttggtatat aacgatgcca gtatcttcct tactctctcc
     5881 gttattaagc ctgatggttt ttatcattgg tacactatac gaatta
//
LOCUS       DAFAVF010000214         5921 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFAVF010000214 DAFAVF010000000
VERSION     DAFAVF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.216"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            42..1175
                     /gene="ompN"
                     /locus_tag="LGK42_004143"
     CDS             42..1175
                     /gene="ompN"
                     /locus_tag="LGK42_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBK2660316.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1316..1750
                     /gene="uspF"
                     /locus_tag="LGK42_004144"
     CDS             1316..1750
                     /gene="uspF"
                     /locus_tag="LGK42_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBK2660317.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1926..2861
                     /gene="ttcA"
                     /locus_tag="LGK42_004145"
     CDS             1926..2861
                     /gene="ttcA"
                     /locus_tag="LGK42_004145"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBK2660318.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2990..4363)
                     /gene="dbpA"
                     /locus_tag="LGK42_004146"
     CDS             complement(2990..4363)
                     /gene="dbpA"
                     /locus_tag="LGK42_004146"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBK2660319.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4841..5824)
                     /gene="zntB"
                     /locus_tag="LGK42_004147"
     CDS             complement(4841..5824)
                     /gene="zntB"
                     /locus_tag="LGK42_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBK2660320.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
BASE COUNT         1563 a         1494 c         1409 g         1455 t
ORIGIN      
        1 tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc aaagtactgg
       61 cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata
      121 aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata
      181 attcggcgaa agatggcgac cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa
      241 ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg
      301 aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt
      361 acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg
      421 atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc
      481 gagccaatgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga
      541 acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag gaaggcacca
      601 acaacggacg tgatgttcgc catgaaaacg gtgacggctg gggtctttcc acaacatatg
      661 atttaggcat gggctttagc gctggtgcgg catacacctc ttctgaccgc accaatgatc
      721 aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat
      781 acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt
      841 ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc actgcacagt
      901 accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
      961 tgcacgctgc cggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     1021 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat gttgactata
     1081 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caatggcatc tctaccgatg
     1141 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     1201 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     1261 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     1321 cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag
     1381 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     1441 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     1501 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     1561 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaaat
     1621 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     1681 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     1741 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     1801 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     1861 atgctgggcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     1921 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     1981 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     2041 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     2101 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     2161 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     2221 agtacaagat tgttgaagag aatacttacg gtatcgtgaa agagaagatt ccagagggca
     2281 aaaccacttg ctcactgtgt tctcgccttc gtcgcggtat cctttatcgt accgcaacgg
     2341 aactgggggc gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     2401 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     2461 atggcaaaca tatcgttatt cgtccgctgg cctactgccg cgagaaagat attcagcgat
     2521 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     2581 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     2641 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     2701 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     2761 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     2821 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     2881 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatccgg
     2941 caatcccgca taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     3001 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     3061 gagcaacagc ctgacgcacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     3121 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     3181 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcggcacaa
     3241 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     3301 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     3361 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     3421 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtagcaacca
     3481 gtacacgggc gctaccgtta gcaaaacgta ccagagtctg atcgcgatcg cgttgctcca
     3541 gatcgccgtg taacgacaat gcactttgcc ctacttcatt cagcgcgtcg cagacagcct
     3601 ggcaatcttt tttggtattg caaaacacca cgcaagagga tggctgatgc aagcttaata
     3661 accgttgcaa cagaggaatt ttgcctttgc tggatgtctc ataaaattgt tgttcaatgg
     3721 gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg atcgcgttgc actcgtccgc
     3781 tgattgcggc gatggcttcc ggccaggttg ccgaaaacag aagcgtctgt cgagatgcag
     3841 gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc catatccagc atgcggtcgg
     3901 cctcatccat caccaaagta ttcagcgcat ccagtgatac cgtgcctttt tgcaagtgat
     3961 ccagcaaacg acccggcgtt gccacgataa tatgcggcgc atgttgcaac gaatcacgct
     4021 gcataccgaa cggttgacca ccgcataacg tcaaaatttt ggtatttggc agaaaacgcg
     4081 ccagccgacg caattcacct gccacctgat ccgccagttc acgcgtagga cacagcacta
     4141 aagcctgggt ttgaaatagc gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg
     4201 ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc ttttccggca aggatcgccg
     4261 gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc caactcatta aggttcgtga
     4321 gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt cacaatctat tctcgtggtc
     4381 atcgacgcaa agttagcagg cgcgtatcct cgcagatcta cgctcacgat gcgacaattt
     4441 aatcggttct tcatcaggtg gtgggtcagg catgggttgc gggcgaggga tcggatcggg
     4501 cactggaaca ggatcgccag gaatcggttc agggacagga atttgcaaat aaataagtgt
     4561 cgtcatattt ccctccggtc attgggtgga ctcttaaagg gtagacgctg ataaataaca
     4621 ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta
     4681 attcaaaaaa ggaagtaaga caatatggag cgcaacgccc atcgcttgac gttgcattca
     4741 cctgcaaggg agatattgcc ctgaatgggt agagagttta ttgacttcgc tcaaactttg
     4801 cggcgttttt gtatacagac agccggaaaa attgcttttg ttacaaccat ttactacggt
     4861 gcaaccataa agcaacacca ccaataagaa caactaacag aatacaaaaa attgaaaatc
     4921 cgaattgcca cccgccgcca gggatcccac caaggttgac gccaaataac cctgtcagaa
     4981 aggtactggg taaaaagacc attgccatca atgacattgt ataggtacga cgagctaaat
     5041 tttcctgcat cacctgagcg atttcatccg ccatcacgcc agtccgtgct atacaggcgt
     5101 cgatttcgtc aagaccgcgc ccaaggcgat cggcaatatc ctgcatccga cggcgttggt
     5161 catcgctcat ccacggcaaa cgttcactgg caagacgagc ataaacatca cgttgcggtg
     5221 ccatatagcg acgcatcacg attaattgtt tgcgcagcag agccaggaat ccacgcggtg
     5281 gaatttgctg atcaaggaga ttatcttcaa ggtcgataat tttatcgtgc agctgctcga
     5341 taaattcgct ggaatgatcg gtcaacgcat cgcacacatc caccagccac cctccgcaat
     5401 cggtcggacc cgtgccctct tccagatcgc tcaccacatc gtccagcgcc agcactttgc
     5461 gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac acgcatggcg accagttgat
     5521 cggggcgttc atcggtgctg ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga
     5581 gacggctgac tcggggccgc gtgctctcgc ccgccagcgc atcacgtacg ttattgggaa
     5641 gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg tacataatta aggtggagcc
     5701 agcagggatg cgcttcatca atcacatctg tattttccag cggtttaacg ccgcctctac
     5761 catccagcat ccaggcaaat actgcatccg ggacattaac gtccgatccc ttaatcgctt
     5821 ccacagtgcc tccatcatca acgcattatt ttgtagtcta gccttctggc cctgttacgc
     5881 aacatctcat caccccatta ccctgaaatg attaataaaa t
//
LOCUS       DAFAVF010000215         5903 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFAVF010000215 DAFAVF010000000
VERSION     DAFAVF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.217"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..330
                     /locus_tag="LGK42_004148"
     CDS             <1..330
                     /locus_tag="LGK42_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBK2660321.1"
                     /translation="HAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAME
                     LAAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQ
                     EQARLKI"
     gene            447..920
                     /locus_tag="LGK42_004149"
     CDS             447..920
                     /locus_tag="LGK42_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660322.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(1397..1663)
                     /locus_tag="LGK42_004150"
     CDS             complement(1397..1663)
                     /locus_tag="LGK42_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660323.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(1663..2157)
                     /locus_tag="LGK42_004151"
     CDS             complement(1663..2157)
                     /locus_tag="LGK42_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660324.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(2213..2815)
                     /locus_tag="LGK42_004152"
     CDS             complement(2213..2815)
                     /locus_tag="LGK42_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBK2660325.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            3169..4515
                     /locus_tag="LGK42_004153"
     CDS             3169..4515
                     /locus_tag="LGK42_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660326.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            4794..>5903
                     /locus_tag="LGK42_004154"
     CDS             4794..>5903
                     /locus_tag="LGK42_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBK2660327.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEV"
BASE COUNT         1599 a         1292 c         1396 g         1616 t
ORIGIN      
        1 catgcaatcg gggaccgacc agtaattatc tttaatgccc ggttcgacat ccgcattctg
       61 aaacagactg ctgccgcaca tagcgatccg gctgactggc tggaagaact gacggtatat
      121 tgtgcgatgg agctggctgc aggatattat ggggccacca accgctatgg cacgatttca
      181 ctggcctgtg ctgccagtca ggccggactg acctgggaag ggcaggcaca ctcagcgatc
      241 gccgacgcac ggatgacggc aggggtggta aacgctattg ctgcatatca tctggaactg
      301 ctgcaggaac aggcacggct gaaaatctga ccgcctggcc tgtataccgc aatcatttac
      361 gttatccaga cgtaaatccg ccgcttgttg ctgtcagcat gttaatgcta atgtatgtgt
      421 acattagcat taatgaggga tgatatgtga gagacaaaac cagggaggcc atgcggcttt
      481 ttttaggcgg caggtgctac acggctgaaa aacttgaaaa ggattatctg gctgaagtgg
      541 ctaattacag taacgaccgc tgggaagcac cgcaacgtgc ctcacgcctg gctgccagcg
      601 taaagcgtta caagacatca gaaatgctgc gctttatttt tgctaccatt gcctacgatc
      661 ccgatccaga tttaaccccg ctaactgtga gacgtttgtg caaggcattg ttcggcagaa
      721 ccggaagcca gtggctggtt gttgaagttt ttggggagaa ggggcgtcag caccgcagcg
      781 cggacagcaa tccggagatg gtggaaaaaa tggctgccag atatcgtcat gccgccgaac
      841 tacactggtc cgcaacgctg gcagaaattg aacgggtaaa gcggctttat cagacgaaaa
      901 ttaagaaatc caaaaaataa gtgggttgac tgccattgtt gttcttctcc ttcatcatta
      961 atgttaatgt atgcatacat tagcattaat gatggtgcgt taaagtatca ctggtataat
     1021 ctggtaaaga acatattgag aatgagaact tatcatgtcg tcacgcaact ctgtacatga
     1081 ttttataatg ctgctcccgg cgctgctgtt ggttgggtgg ttagttttgg tgataattct
     1141 catgtttagg gatgataaat gtgacaaaac ttaccgtgaa ggttcgtttc ccgaatttat
     1201 ttgtctccat tctgatcgct cgtcacttcg agaaaccgat tctgagagac tcattgtcaa
     1261 atatacagaa ataaaatcac aaaaataggt aaaaccaata atactatagc taaaaaatag
     1321 ttacttttct gtatcaccct gtctctctct gctttttcat ggctactgtc atatttatgt
     1381 ctgttgcgcg gttttgttac tttttcatga ccgaacccga aattttgcgc gtcaataaga
     1441 gacgttgtgt aaactcagtc cttttatctg gcaggttaac ccctatcaat cttaggatga
     1501 aaccacgcca tcctctgttt gcctggttat taatttcata aagacgattg ttttcctctc
     1561 gtagttttgt ccactctaaa caaagtgtta tatcatccag ttctgtgatg tgtttcactt
     1621 ttccattgac agttacagtg tttattccat ttcctgtgag catcatctta tgcctttttt
     1681 atcttcattt gggaccgtgc ttttcttcgg agtatcacta ttcgttaact gctgtttatc
     1741 aataaaattc ctgatgttat caagtgcttc ctttgttttt agaatgcgct tgatatccat
     1801 ttcatcccgc ccggtcattc tttttccgga caaaagacgc tcaagaagct cctgtttcgc
     1861 atcaggagaa tctttggcga gatccatcag gttaagcaaa tcttcacgat cgagaggaaa
     1921 gaagtatttt acaaaatact tgaaaagcga aagaaagtag tatgctgtcc atgcaataca
     1981 gaaaccgatt ctgagaaact cattgtcaac tatacagaaa tacaggtata caatcaggag
     2041 aataggtaaa atccataata ctatagctaa aaaaatatat acggggatca ggcgtctgta
     2101 tgcccgttca tgtttattca ggatgaataa aatcttctcc gcttctgaat cacacataac
     2161 attctcttcc cattttttct gatacgtaat gtccacctgt tatcaggagt gtttattgcc
     2221 cagaaatatt ctcctgctgt gcctgcgtcc tgagccactt ttgttctaat gtcgccagtc
     2281 tgcgaccggc actctgtgct tcagagtctg agacctgccc tttggggtga ccatccaggc
     2341 cataacgtgg gacgccggcg acagctgtcc gctgcagata tacgcggcgg gatgtgatgc
     2401 aggacactgc acagagccat tcgtccatgg tcagttctgt ctccgggtgt tctgccagaa
     2461 atgccgaaat atcattgctt atgccaattt tcaggggaat aaatccacca ccttcagcac
     2521 gaagatgagg aaacagggcc tttatttttt ccacgcactc gtgagtatac tcacgggtcc
     2581 agtgggaggg acgtggaaaa cggtgtcttt tctttttctt cttcctggct ggcgcttcgg
     2641 ggatatgtgt ttcgacggca gatgaattta ttccggcggg ggctgattct gccgtctgtt
     2701 cagtaaatac aggtgcgata atatctgttt tttcggataa ggacggtggc gaaaaagtac
     2761 gacgtttttt caccacaatg acgggagttt tcctttcttc ctgatttttt cccataaata
     2821 cctcggctgg ctggttttac tctttaataa tatcacaatc cataaattag taaatttccc
     2881 atcaggtgat ttttcatggc taataagtcc attctctgaa tatttatact cgaggtagtt
     2941 acagttatgg cgtgacagtc gaattgatat tctactgaac cctgacagaa acatggttga
     3001 tatattctct cactttaatg ttgtcatgat ataaataaaa acaattcatt tgaaaggata
     3061 tcagaagaag atatcggggc gcacagttca tctctgcctt tgtttcatgg ggggttcttt
     3121 gtatatttta cttactgttt aagcatgtac ataaggagtg ctttcattat gccaatgata
     3181 cttgtaaaga agaatccccg gggcaaagtg attcgggagc ttagcagtga ggaagagact
     3241 gcagtaaaga cggtctgtgg actgaaaagg ccagcaacca tggcacaaca taacctggcc
     3301 aatgatcttc tccgggaaat gcgtgagtat gatgcatggt tacagtgtga ctgtattccg
     3361 ggagattcgc cggccatgaa ttttgctgct cttaaaaaca atacgggtac tctttatctg
     3421 tccagtttta atcatgagca tgcacctgaa tgccccatgt atcgtcagtt atccggaaat
     3481 gaagaggaaa catccttcgg agcaagccgg catcccgtta gcacccgtat aaattacaga
     3541 aactttttac cacctgatga cagtaattct gttatcagac tgcaggcccg ctctgcttac
     3601 cataatggtg agcgctcctc cgttcgcaag aaacgtccgc gccttggccg tttactcctg
     3661 tcgcttattg aagatgcagg gctgaataaa ctggattccc tggctaatcc ccgtatccgg
     3721 actaattatc gtgagtgtct tgatgccata cggcaggtta cactacagca ggagtatatt
     3781 cggggaagag ccttatctga aattatccat ttccggccag gaatgtctga aaggtctcag
     3841 gagcggttaa tggaaacgct ggagaattca gaacgacact ggcctgccag aagaaagcat
     3901 atgttctttc agatttttat ggcgcagcac atctgtcgtg atgctgtgga aattcactgg
     3961 gcgaacggta atattcaggt catcagacct gtgcggggga tcagtattaa tggtgaagcg
     4021 cagggcggaa tacgtccccc ttactgggtt attcttgctt tttgccggag tgccgatggc
     4081 agaatcatct gcagtgaagg gtatgctcat gctctttatc aactgacatg tccggtgcct
     4141 gtggacagca aactggaacg aaacacgctc actgctcttc tgaatgtggc cagctggctt
     4201 aaaagaaagc caggtacgcc ggaattaagt ctggaaaggc ccctgtttga tacagaagtt
     4261 tatgttaatg gtgaaaagaa atatgtactg ccggatttca ttgtcacagc aagggctcct
     4321 gacggaaaga cggccagagt ggtcatcgaa acgatgggat atgaagacag tgattactgc
     4381 gcgagaaaat ccaggcagca taccggcatg aagcagattg gtgttctgca taccgatcca
     4441 ccgaaatggc tggataacga tcatccccct tttaagaaac atatgtacgg tgtttttatg
     4501 catctcaggt actgagatat tttgtggctc agttctgtaa cttttcccgt aacattgtct
     4561 gttgttacgg gaaagtccgg tttttgtatt gcaccagaga atacccagac tgtgatgctg
     4621 ccacagtgtc agcaggcttt ctgaacggta taacaccact tcatgttaat gataattact
     4681 atcattaaat cttgacatgc cattttctcc ttaataaatt agtactgtat atgtatccat
     4741 atacgtaagc agttaattca tttgttttcc tcagaggatg aaggagatac cgaatgtctg
     4801 accctgtacg tattacaaat cccggtgcag aatcgctggg atatgattca gatggccatg
     4861 aaattatggc cgttgatatt tatgtaaacc ctccacgtgt cgatgtcttt catggtaccc
     4921 cgcctgcatg gagttccttc gggaacaaaa ccatctgggg tggaaacgag tgggtcgatg
     4981 attccccaac ccgaagtgat atcgaaaaaa gggacaagga aatcacagcg tacaaaaaca
     5041 cgctcagcgc gcagcagaaa gagaatgaga ataagcgtac tgaagctgga aaacgccttt
     5101 ctgcggcaat tgctgcaagg gaaaaagatg aaaacacact gaaaacactc cgtgccggaa
     5161 acgcagatgc cgctgatatt acacgacagg agttcagact cctgcaggca gagctgagag
     5221 aatacggatt ccgtactgaa atcgccggat atgatgccct ccggctgcat acagagagcc
     5281 ggatgctgtt tgctgatgct gattctcttc gtatatctcc ccgcgaggcc aggtcgttaa
     5341 tcgaacaggc tgaaaaacgg cagaaggatg cgcagaacgc agacaagaag gccgctgata
     5401 tgcttgctga atacgagcgc agaaaaggta ttctggacac gcggttgtca gagctggaaa
     5461 aaaatggcgg ggcagccctt gccgttcttg atgcacaaca ggcccgtctg ctcgggcagc
     5521 agacacggaa tgacagggcc atttcagagg cccggaataa actcagttcg gtgacggaat
     5581 cgcttaagac ggcccgtaat gcattaacca gagctgaaca acagctgacg caacagaaaa
     5641 acacgcctga cggcaaaacg atagtttccc ctgaaaaatt cccggggcgt tcatcaacaa
     5701 atcattctat tgttgtgagt ggtgatccga ggtttgccgg tacgataaaa atcacaacca
     5761 gcgcggtcat cgataaccgt gcaaacctga attatcttct gacccattcc ggtctggact
     5821 ataaacgcaa tattctgaat gaccggaatc cggtggtgac agaggatgtg gaaggtgaca
     5881 agaaaattta taatgctgaa gtt
//
LOCUS       DAFAVF010000216         5788 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFAVF010000216 DAFAVF010000000
VERSION     DAFAVF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.218"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(108..1079)
                     /locus_tag="LGK42_004155"
     CDS             complement(108..1079)
                     /locus_tag="LGK42_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HBK2660328.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1079..2245)
                     /gene="sopA"
                     /locus_tag="LGK42_004156"
     CDS             complement(1079..2245)
                     /gene="sopA"
                     /locus_tag="LGK42_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HBK2660329.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2833..3588)
                     /gene="repE"
                     /locus_tag="LGK42_004157"
     CDS             complement(2833..3588)
                     /gene="repE"
                     /locus_tag="LGK42_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HBK2660330.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4310..5116)
                     /locus_tag="LGK42_004158"
     CDS             complement(4310..5116)
                     /locus_tag="LGK42_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBK2660331.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(5117..5422)
                     /gene="ccdB"
                     /locus_tag="LGK42_004159"
     CDS             complement(5117..5422)
                     /gene="ccdB"
                     /locus_tag="LGK42_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBK2660332.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(5424..5642)
                     /gene="ccdA"
                     /locus_tag="LGK42_004160"
     CDS             complement(5424..5642)
                     /gene="ccdA"
                     /locus_tag="LGK42_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBK2660333.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
BASE COUNT         1598 a         1460 c         1280 g         1450 t
ORIGIN      
        1 agtggaacag tgggcccaga gagaatattc aggccagtta tgctttctgg cctgtaacaa
       61 aggacattaa gtaaagacag ataaacgtag actaaaacgt ggtcgcatca gggtgctggc
      121 ttttcaagtt ccttaagaat ggcctcaatt ttctctatac actcagttgg aacacgagac
      181 ctgtccaggt taagcaccat tttatcgccc ttatacaata ctgtcgctcc aggagcaaac
      241 tgatgtcgtg agcttaaact agttcttgat gcagatgacg ttttaagcac agaagttaaa
      301 agagtgataa cttcttcagc ttcaaatatc accccagctt ttttctgctc atgaaggtta
      361 gatgcctgct gcttaagtaa ttcctcttta tctgtaaagg ctttttgaag tgcatcacct
      421 gaccgggcag atagttcacc ggggtgagaa aaaagagcaa caactgattt aggcaatttg
      481 gctgtgttga tacagcgggt aataatctta cgtgaaatat tttccgcatc agccagcgca
      541 gaaatatttc cagcaaattc attctgcaat cggcttgcat aacgctgacc acgttcataa
      601 gcacttgttg ggcgataatc gttacccaat ctggataatg cagccatctg ctcatcatcc
      661 agctcgccaa ccagaacacg ataatcactt tcggtaagtg cagcagcttt acgacggcga
      721 ctcccatcgg caatttctat gacaccagat actcttcgac cgaacgccgg tgtctgttga
      781 ccagtcagta gaaaagaagg gatgagatca tccagtgcgt cctcagtaag cagctcctgg
      841 tcacgttcat tacctgacca tacccgagag gtcttctcaa cactatcacc ccggagcact
      901 tcaagagtaa acttcacatc ccgaccacat acaggcaaag taatggcatt accgcgagcc
      961 attactccta cgcgcgcaat taacgaatcc accatcgggg cagctggtgt cgataacgaa
     1021 gtatcttcaa ccggttgagt attgagcgta tgttttggaa taacaggcgc acgcttcatt
     1081 atctaatctc ccagcgtggt ttaatcagac gatcgaaaat ttcattgcag acaggttccc
     1141 aaatagaaag agcatttctc caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa
     1201 aaacagttct catccggatc tgacctttac caacttcatc cgtttcacgt acaacatttt
     1261 ttagaaccat gcttccccag gcatcccgaa tttgctcctc catccacggg gactgagagc
     1321 cattactatt gctgtatttg gtaagcaaaa tacgtacatc aggctcgaac cctttaagat
     1381 caacgttctt gagcagatca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcaa
     1441 acaactcagc aggcgtggga acaatcagca catcagcagc acatacgaca ttaatcgtgc
     1501 cgatacccag gttaggcgcg ctgtcaataa ctatgacatc atagtcatga gcaacagttt
     1561 caatggccag tcggagcatc aggtgtggat cggtgggcag tttaccttca tcaaatttgc
     1621 ccattaactc agtttcaata cggtgcagag ccagacagga aggaataatg tcaagccccg
     1681 gccagcaagt gggctttatt gcataagtga catcgtcctt ttccccaaga tagaaaggca
     1741 ggagagtgtc ttctgcatga atatgaagat ctggtaccca tccgtgatac attgaggctg
     1801 ttccctgggg gtcgttacct tccacgagca aaacacgtag ccccttcaga gccagatcct
     1861 gagcaagatg aacagaaact gaggttttgt aaacgccacc tttatgggca gcaaccccga
     1921 tcaccggtgg aaatacgtct tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat
     1981 gattaatttg ttcaattgta taaccaacac gttgctcaac ccgtcctcga atttccatat
     2041 ccgggtgcgg tagtcgccct gctttctcgg catctctgat agcctgagaa gaaaccccaa
     2101 ctaaatccgc tgcttcacct attctccagc gccgggttat tttcctcgct tccgggctgt
     2161 catcattaaa ctgtgcaatg gcgatagcct tcgtcatttc atgaccagcg tttatgcact
     2221 ggttaagtgt ttccatgagt ttcattctga acatccttta atcattgctt tgcgtttttt
     2281 tattaaatct tgcaatttac tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg
     2341 caaagttgtt taaaataaga gcaacactac aaaaggagat aagaagagca catacctcag
     2401 tcacttatta tcactagcgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc
     2461 gaggaagcaa agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata
     2521 tccaccgtgg gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata
     2581 aactgtgata atcaaccctc atcaatgatg acgaactaac ccccgatatc aggtcacatg
     2641 acgaagggaa agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa
     2701 aaattacagt tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt
     2761 gacaaattac cctcagtagg tcagaataaa tgtgacgaac caccctcaaa tctgtgacag
     2821 ataaccctca gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg
     2881 agtcgtctgg cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt
     2941 gatctcatta acacagacct gcaggaagcg gcggcggaag tcaggcatac gctggtaact
     3001 ttgaggcagc tggtaacgct ctatgatcca gtcgattttc agagagacga tgcctgagcc
     3061 atccggctta cgatactgac acagggattc gtataaacgc atggcatacg gattggtgat
     3121 ttcttttgtt tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg
     3181 aatgagatat gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt
     3241 gataaaccag ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata
     3301 aaaaaccact tccttccccg cgaaactctt caatgcctgc cgtatatcct tactggcttc
     3361 cgcagaggtc aatccgaata tttcagcata tttagcaaca tggatctcgc agataccgtc
     3421 atgttcctgt agggtgccat cagattttct gatctggtca acgaacagat acagcatacg
     3481 tttttgatcc cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg
     3541 cgggctattt ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg
     3601 tgaagtgtga caagttttta gattgtcaca ctaaataaaa aagagtcaat aagcagggat
     3661 aactttgtga aaaaacagct tcttctgagg gcaatttgtc acagggttaa gggcaatttg
     3721 tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc taaaaaagaa
     3781 gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag tggataaccc
     3841 caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg ttccctggtg
     3901 cttcctcgct cactcgaggg cttcgccctg tcgctcgact gcggcgagca ctactggctg
     3961 taaaaggaca gaccaaacat catggttctg tgttcattag gttgttctgt ccattgctga
     4021 cataatccgc tccacttcaa cgtaacaccg cacgaagatt tctattgttc ctgaaggcat
     4081 attcaaatcg ttttcgttac cgcttgcagg catcatgaca gaacactact tcctataaac
     4141 gctacacagg ctcctgagat taataatgcg gatctctacg ataatgggag attttcccga
     4201 ctgtctcgtt cgcttctcag tggataacag ccagcttctc tgtttaacag acaaaaacag
     4261 catatccact cagttccaca tttccatata aaggccaagg catttattct caggataatt
     4321 gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg tgccgggcag
     4381 ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga actgatggac ttatgtccca
     4441 tcaggctttg cagaactttc agcggtatac cggcatacag catgtgcatc gcataggaat
     4501 ggcggaacgt atgtggtgtg accggaacag agaacgtcac accgtcagca gcagcggcgg
     4561 caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt
     4621 tctctgtcct tcctgtgcga cggttacgcc gctccatggg tattttcagt gttgccacca
     4681 tcgtctgcag ctggctgacg taccaggagt cagagagcgg aaccagccgg tgagtctgct
     4741 gaccggcggg cattctcccc gccgtcctgg cagctttttc ggtccgttgt ttcagggtcg
     4801 caagctgcac aaacggatac ggaggcgtga gcgaaaaatc cccccgcgtc agcgctagtg
     4861 cttcattaat acgcgcgccg gtgttccaca gagtggccag cagcatcttg cggcgcagat
     4921 ccgggacata atgaagcagg gcactcacct cgggagccag cagatatttc ggcagttcat
     4981 catgaaccat cgacatctgg cggagtgcca gagctgccgg ataatcaatg gcaacaggaa
     5041 gttgtgcaga ctgtccggca gaatacactg ccggtaccat ggctgcagac tggctgtgta
     5101 taacggagcc tgacatttat attccccaga acatcaggtt aatggcattt ttgatgtcat
     5161 tttctcggtg gctgagatca gccacttcct ccccgataac ggagaccggc acactggcca
     5221 tatcggtggt catcatgcgc cagctttcat ccccgacatg caccaccggg taaagctcac
     5281 gggagacctt atctgacaac agacgtgcac tggccagggg gatcaccatc cgtcgcccag
     5341 gcgtgtcaat aatgtcactc tggacatcca caaacagacg ataacggctc tctcttttat
     5401 aggtgtaaac cttaaactgc atttcaccag tccctgttct cgtcggcaaa agagccgttc
     5461 atttcaataa accgggcgac ctcagccatc ccttcctgat tttctgcctg ccagcgttcg
     5521 gcacgcagac gacgggcttc attctgcagg gtggtgctta ccagcccgga gatattgaca
     5581 tcatatgcct tgagcaactg atagctgtca ctgtcgacgg tgactgtaat gcgctgcttc
     5641 ataatgcacc tcatcttaca tactctctgt acaccattaa gtatacacca gtattacgct
     5701 ataacatgcc tccggcaacg gaatattgtt atccagctat tattaacacc accatcaata
     5761 actgacctga aaagtaatca tgacaata
//
LOCUS       DAFAVF010000217         5754 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFAVF010000217 DAFAVF010000000
VERSION     DAFAVF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.219"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            59..601
                     /gene="fimA"
                     /locus_tag="LGK42_004161"
     CDS             59..601
                     /gene="fimA"
                     /locus_tag="LGK42_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2660334.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRIDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            821..1513
                     /gene="fimC"
                     /locus_tag="LGK42_004162"
     CDS             821..1513
                     /gene="fimC"
                     /locus_tag="LGK42_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBK2660335.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1544..4153
                     /locus_tag="LGK42_004163"
     CDS             1544..4153
                     /locus_tag="LGK42_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBK2660336.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4166..5173
                     /gene="fimH"
                     /locus_tag="LGK42_004164"
     CDS             4166..5173
                     /gene="fimH"
                     /locus_tag="LGK42_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBK2660337.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5184..5699
                     /gene="sfmF"
                     /locus_tag="LGK42_004165"
     CDS             5184..5699
                     /gene="sfmF"
                     /locus_tag="LGK42_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBK2660338.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
BASE COUNT         1513 a         1244 c         1385 g         1612 t
ORIGIN      
        1 ataaagtaat cggttatata cggatgtgga gtcgataaat gagattgaag gaatatatat
       61 gaaattaaga tttatttcgt ctgcgctggc tgccgcacta ttcgccgcta cgggtagtta
      121 tgctgccgtt gtagatggcg gtacaattca ctttgaaggc gaactggtga atgctgcctg
      181 ttcagtgaat actgactcgg cagaccaggt tgtcacactc ggtcaatatc gtatcgatat
      241 tttcaatgct gttggtaata cctctgcatt aattccattc accattcagt tgaacgactg
      301 cgatcctgtt gttgccgcta atgctgccgt tgcattttct ggtcaggctg atgcaatcaa
      361 tgataattta ttggccattg catccagtac caatacaaca acagcaacgg gtgtcggtat
      421 tgaaatactt gataatacat ccgcaattct caaacctgat gggaatagct tctcaaccaa
      481 ccagaacttg atccccggga ccaacgttct tcatttttct gcacgttata aaggcaccgg
      541 tacaagtgca tcagcagggc aagcaaatgc tgacgcgact tttattatga gatacgaata
      601 atcaaaacca cgttgttttg aattatatat cacgtcttat aacaaagtaa tgtaccggtt
      661 gtctgaagcg gtatggtggc aatgtaaatc gaaatcatgt tcactttgta tcatgccgct
      721 ttattaaatg aaaagggaat gatgtgttgt aagaaaccaa agcaatcatt tctttatatt
      781 tcttattttt gccgtcagga atacacaagg cgtattaact atgatgacta aaataaagtt
      841 attgatgctc attatatttt atttaatcat ttcggccagc gcccatgctg ccggagggat
      901 cgcattaggt gccacgcgta ttatttatcc cgctgatgct caacagactg cggtatggat
      961 tagaaatagc cataccaatg agcgctttct ggtcaattcg tggattgaaa acagcagcgg
     1021 tgtaaaagaa aagtcattca tcattacacc gccactgttt gttagtgaac ccaaaagcga
     1081 aaataccttg cgtattattt acaccggtcc accgctggcg gcagatcgtg agtctctgtt
     1141 ctggatgaat gttaagacga tcccttcggt agataaaaat gcattgaacg gcaggaatgt
     1201 tttgcaactg gcgattttat cgcgcatgaa attatttctc cgtccaattc aattacaaga
     1261 attacccgca gaagcgccgg acacactcaa gttttcgcga tccggtaact atatcaatgt
     1321 tcataatccc tcaccttttt atgtcaccct ggttaactta caagtgggca gccaaaagtt
     1381 ggggaatgct atggctgcac ccagagttaa ttcacaaatt cccttaccct caggagtgca
     1441 gggaaagctg aaatttcaga ccgttaatga ttatggttca gtaaccccgg tcagagaagt
     1501 gaacttaaac taaccgaatc atctgacaat atcagagcta attatgaaaa tacccactac
     1561 tacggatatt ccgcagaggt atacctggtg tctggccgga atttgttatt catctcttgc
     1621 cattttaccc tcctttttaa gctatgcgga aagttatttc aacccggcat ttttattaga
     1681 gaatggcaca tccgttgctg atttatcgcg ctttgagaga ggcaatcatc aacctgcggg
     1741 agtgtatcgg gtggatctct ggcgtaatga tgagttcatt ggttcacagg atatcgtatt
     1801 tgaatcgaca acagaaaata caggtgataa atcaggtggg ttaatgccct gttttaacca
     1861 ggtactcctt gaacgaattg gccttaatag cagtgcattt cccgagttag cccagcagca
     1921 aaacaataaa tgcatcaatt tactgaaagc tgtacctgat gccacaatta actttgattt
     1981 tgcagcgatg cgcctgaaca tcactattcc tcagataacg ttgttgagta gcgctcacgg
     2041 ttacattccg cctgaagagt gggatgaagg tattcctgct ttactcctga attataattt
     2101 caccggtaac agaggtaatg gtaacgatag ctattttttt agtgagctca gcgggattaa
     2161 tattggcccg tggcgtttac gcaacaatgg ttcctggaac tattttcgcg gaaatggata
     2221 tcattcagaa cagtggaata atattggcac ctgggtacag cgcgccatta ttccgctgaa
     2281 aagtgaactg gtaatgggag acggcaatac aggaagtgat attttcgatg gtgttggatt
     2341 tcgtggtgta cggctttatt cttccgataa tatgtatcct gatagccagc aagggtttgc
     2401 cccaacggta cgtgggattg cccgtacggc ggcccagcta acgattcggc aaaatggttt
     2461 tattatctat caaagctatg tttcccccgg cgcttttgaa attacagatt tgcacccgac
     2521 atcttcaaat ggcgatctgg acgtcaccat cgacgagcgc gatggtaatc agcagaatta
     2581 cactatcccg tattcaacag tgccaatttt acaacgcgaa gggcgtttca aatttgacct
     2641 gacggcgggc gattttcgta gcggtaatag tcagcaatca tcgcctttct tttttcaggg
     2701 cacggcactc ggcggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc
     2761 caattacacc gcctttttat tagggctggg gcgcaacctc gggaactggg gggcagtgtc
     2821 gctggatgtg acccatgcgc gcagtcagtt agccgacgac agtcgtcatg agggggattc
     2881 cattcgcttc ctctatgcga aatcgatgaa caccttcggc accaattttc agttaatggg
     2941 ttaccgctat tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga
     3001 ggggtacgaa tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa
     3061 ttaccacaat ttacgcttta gccgtaaaga ccgtttgcag ttaaatattt cacaatcact
     3121 taatgacttt ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga
     3181 ttcagatacc tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcgct
     3241 ctcattttcg tggaatgaat ctgtagggat ccccgataac gaacgtattg tcggacttaa
     3301 tgtttcagtg cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga
     3361 ccgcgcttat gcctccttta acgccaaccg taacagcaac gggcaaaata gctggctggc
     3421 aggtgtaggt gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggtga
     3481 tacctcgaat aatgggtata cgggcagcgc cacggcaaac tggcaggcca cttacggtac
     3541 gctgggggtc gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg
     3601 cggtgtggtc gggcacgaaa atggcataac gctgagccag cctttagggg ataccaatgt
     3661 tttgattaaa gcgcctggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac
     3721 cgactggcgc ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc
     3781 gcttgatacc aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt
     3841 gccgacgcaa ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct
     3901 cattaccgtt acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag
     3961 taccggaata accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt
     4021 gtctggtgaa ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta
     4081 tgtattgccg aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca
     4141 tcctggctca taaaggaaat tatcaatgaa gataatctgc agattattat tggcgatggc
     4201 atgtttgtgt ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc
     4261 agaagatgaa cactatgatc tctcaaatat ctttaatagc accaataacc agccagggca
     4321 gattgttgtt ttaccggaaa aatccggctg ggtaggtgtg tcagcaattt gtccacccgg
     4381 cacgctggtg aattatacat accgtagtta tgtcaccaac tttattgttc aggaaactat
     4441 cgataattat aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga
     4501 tagtgtgatg gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa
     4561 cgtttcacaa aaccttccat tcggggtgat ggattctcgt ttaatatttc gtttaaaggt
     4621 tattcgtccc tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt
     4681 gacatcaacg ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg
     4741 ggttgaagta ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg
     4801 tgatatcggc gcatcgttat ttagtgcggc agggccgggt aatcgacctg ctggtgtcat
     4861 gccgcaaacc aagagcattg cggtcaaatg tacgaatgtt gctgcgcagg cttatttaac
     4921 aatgcgtctg gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt
     4981 aggttttatt gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt
     5041 tattcctttc cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat
     5101 cagtattacc ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt
     5161 cgattatcaa tgaggtacgg agaatgagaa gagcactctt tagctgtttc tgcgggttac
     5221 tgtggatttc cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg
     5281 gtaacgttgt tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt
     5341 taggcagatg gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt
     5401 ttagcctgcg gctggaggga tgtcctccgg gttcagttgc gattttattt acgggaacgc
     5461 cggcatccga taccaacctg ctggctctgg atgatcccgc aatggcgcaa accatcgcca
     5521 tcgaattacg taatagcgat cgctcccggc tcgcactggg agcggcgagc ccgactgagg
     5581 aagtagatgc aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca
     5641 gcggtgttcg gccaggtgtg gcgaaagcgg atgcgatatt tatgatcaat tataattaat
     5701 attatattaa ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgca
//
LOCUS       DAFAVF010000218         5720 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFAVF010000218 DAFAVF010000000
VERSION     DAFAVF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.220"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..984
                     /gene="ldhA"
                     /locus_tag="LGK42_004166"
     CDS             <1..984
                     /gene="ldhA"
                     /locus_tag="LGK42_004166"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBK2660339.1"
                     /translation="LAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCE
                     AVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEA
                     VAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRI
                     LKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAF
                     DQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQD
                     DVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1095..1517
                     /gene="hslJ"
                     /locus_tag="LGK42_004167"
     CDS             1095..1517
                     /gene="hslJ"
                     /locus_tag="LGK42_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBK2660340.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1514..1780)
                     /locus_tag="LGK42_004168"
     CDS             complement(1514..1780)
                     /locus_tag="LGK42_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBK2660341.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2054..5578
                     /gene="nifJ"
                     /locus_tag="LGK42_004169"
     CDS             2054..5578
                     /gene="nifJ"
                     /locus_tag="LGK42_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK2660342.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1425 a         1478 c         1480 g         1337 t
ORIGIN      
        1 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
       61 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
      121 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa
      181 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
      241 gaccttgacg cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca
      301 gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
      361 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
      421 tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt
      481 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg
      541 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
      601 ctgcactgcc cgctgacacc ggaaaactac catctgttga acgaagccgc cttcgatcag
      661 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca
      721 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac
      781 gaacgcgatc tattctttga agataaatcc aacgacgtaa ttcaggatga cgtattccgt
      841 cgcctgtctg cctgccacaa cgtgctattt accgggcacc aggcattcct gacagcagaa
      901 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa
      961 acctgcccga acgaactggt ttaatcttgc cgctcccctg cattccaggg gagctgattc
     1021 agataatccc caatgacctt tcatcctcta ttcttaaaat agccctgagt cagaaactgt
     1081 aattgagaac cacaatgaag aaagtagccg cgctcgttgc gctaagcctg ctgatggcgg
     1141 gatgtgtaag taatgacaaa attgctgtaa cgccagaaca gttacagcat catcgttttg
     1201 tgctggaaag cgtaaacggt aagcccgtga ccaacgataa aaatccgcca gaaatcagct
     1261 ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     1321 tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     1381 cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg
     1441 atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg
     1501 cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt tcactacagc
     1561 gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc atccagttga cgggcaacag
     1621 aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta
     1681 aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca
     1741 acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc
     1801 aagccaaacg acaccgccat aaataatagg cagcacagag ggcgacgtcg agagctgtcc
     1861 tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa
     1921 aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc
     1981 taaaattgaa ggattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat
     2041 gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg
     2101 caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc
     2161 tgatgcctgg gccggaaacg gcttaaagaa cgtttgggga gacacaccac gcgtagttga
     2221 aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct
     2281 ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacgc tgtacaaact
     2341 ggcaggcgaa ctgacgccat ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc
     2401 actttctatt tttggcgatc attccgacgt tatggcggtg cgccagacgg gttgcgcgat
     2461 gttgtgtgcg gctaacgtcc aggaagcgca agattttgct ctcatttcgc acatcgcaac
     2521 gctgaagagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat
     2581 caataaaatt gtcccgctgg ccgatgacac tattcttgat ctcatgccgc aggccgaaat
     2641 tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc
     2701 caatcctgac acttatttcc agtctcgcga agccaccaac ccatggtacg acgcggtcta
     2761 tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc
     2821 gtttgaatat tacgggcatc cgcaagcgga acgggtgatt atcctgatgg gctctgccat
     2881 tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct
     2941 gaaagttcgc ctgtaccgtc ccttctccgc caaacacttg ctgcaagctc tgccgggatc
     3001 cgtacgcagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct
     3061 ctatctggat gtaatgaccg cactggcaga agcctttaat aatggcgagc gcgaaactct
     3121 gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt
     3181 actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat
     3241 ttacgatgat gtgaccaatc tgtcactgcc gttgccggaa aacaccctgc caaactcggc
     3301 gaaactggaa gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa
     3361 caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga
     3421 ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg
     3481 ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga
     3541 taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta
     3601 cagcgcagat gaagtgtggt cgcgcttgcc gcaggaagtt caggccgtgc taaatcagaa
     3661 aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc
     3721 ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga
     3781 tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga
     3841 tctggtggaa cgcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc
     3901 gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc
     3961 tgatttcgtg aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt
     4021 ttcggcgctg ccgccagacg gcacctggcc gatgggcacc actcgctggg aaaaacgcaa
     4081 tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt
     4141 cgccgcttgc ccacactcag ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa
     4201 cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata
     4261 tgtcttgcag gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc
     4321 ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca
     4381 tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag
     4441 caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc
     4501 aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg
     4561 cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc
     4621 ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctgtt
     4681 tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg
     4741 cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac tgacagcgtt
     4801 gaaatcagac gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact
     4861 caacgatgtt gccgaagcac atgaactgct gcgtgacgca gatgcactgg tggaaaaatc
     4921 aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca
     4981 tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa
     5041 caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca
     5101 cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta
     5161 tgtggcgcag atttctctcg gcgcgcagct caaccagacg gtgaaagcga ttcaggaagc
     5221 ggaagcgtat ccggggccat cgctgatcat cgcttatagc ccatgtgaag agcatggtta
     5281 cgatctggca ctcagccacg accagatgcg ccaactcaca gctaccggct tctggccgct
     5341 atatcgcttc gatccgcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg
     5401 cccgccgtca gtagcactgg aagaaacgtt acttcacgag caacgtttcc gtcggctgaa
     5461 ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa
     5521 acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat
     5581 gctctggata aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct
     5641 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
     5701 aataaaggca tataacccgc
//
LOCUS       DAFAVF010000219         5708 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFAVF010000219 DAFAVF010000000
VERSION     DAFAVF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.221"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            1..204
                     /gene="yneK"
                     /locus_tag="LGK42_004170"
                     /pseudo
     CDS             1..204
                     /gene="yneK"
                     /locus_tag="LGK42_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
     gene            354..1544
                     /gene="ydeA"
                     /locus_tag="LGK42_004171"
     CDS             354..1544
                     /gene="ydeA"
                     /locus_tag="LGK42_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBK2660343.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIAALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(1569..2234)
                     /gene="marC"
                     /locus_tag="LGK42_004172"
     CDS             complement(1569..2234)
                     /gene="marC"
                     /locus_tag="LGK42_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBK2660344.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2446..2880
                     /gene="marR"
                     /locus_tag="LGK42_004173"
     CDS             2446..2880
                     /gene="marR"
                     /locus_tag="LGK42_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBK2660345.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2900..3283
                     /gene="marA"
                     /locus_tag="LGK42_004174"
     CDS             2900..3283
                     /gene="marA"
                     /locus_tag="LGK42_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBK2660346.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3315..3533
                     /gene="marB"
                     /locus_tag="LGK42_004175"
     CDS             3315..3533
                     /gene="marB"
                     /locus_tag="LGK42_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBK2660347.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3590..5029)
                     /locus_tag="LGK42_004176"
     CDS             complement(3590..5029)
                     /locus_tag="LGK42_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK2660348.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYAQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5054..>5708)
                     /locus_tag="LGK42_004177"
     CDS             complement(5054..>5708)
                     /locus_tag="LGK42_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBK2660349.1"
                     /translation="TDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVAN
                     NSYASSFASFSDASNTMAHDRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHG
                     EDVYSYESGAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYK
                     TTLWHALKVGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT         1501 a         1407 c         1263 g         1537 t
ORIGIN      
        1 tctgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca aacatatggc
       61 gcgcaggacc ctcaaactgt ttgtaaaaat gaaacgccgc caaaagttac tcacgaagac
      121 ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta tacccgcaat cgccaaaacg
      181 ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga ctctctgacg cggaacagaa
      241 tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa tgcattgtat tcacatcatt
      301 aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct gttatgacaa
      361 caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct
      421 tcatcttcaa caccaccgaa tttgtccctg ttggcctgct ctctgacatt gcgcaaagtt
      481 ttcacatgca aaccgctcag gtcggcatta tgttgaccat ttatgcatgg gtagtggcgc
      541 taatgtcatt gccttttatg ttaatgacca gccaggttga acggcgcaaa ttactgatct
      601 gcctgtttgt ggtgtttatt gccagccatg tactgtcgtt tttatcgtgg agctttaccg
      661 ttctggtgat cagtcgcatt ggtgtggctt ttgcgcatgc gattttctgg tcgattacgg
      721 cgtctctggc gatccgtatg gctccggccg ggaagcgagc acaggcattg agtttaattg
      781 ccaccggtac agcactggcg atggtcttag gtttacctct cgggcgtatt gtgggccagt
      841 atttcggttg gcgaatgacc ttcttcgcga ttggtattgc ggcgcttatc acccttttgt
      901 gcctgattaa gttacttccc ttactgccca gtgagcattc cggttcattg aaaagcctcc
      961 cgctattgtt ccgccgcccg gcattgatga gcatttattt gttaactgtg gtggttgtca
     1021 ccgcccatta cacggcatac agctatatcg agccttttgt gcaaaacatt gcgggattca
     1081 gcgccaactt tgccacggca ttactgttat tactcggtgg tgcgggcatt attggcagcg
     1141 taattttcgg taaactgggt aatcagtatg cgtctgcgtt ggtaagtacg gcgattgcgc
     1201 tgttgctggt gtgcctggca ctgctgctac ctgcggcgaa cagtgaaata cacctcgggg
     1261 tgctgagtat tttctggggg atcgcgatga tgatcatcgg gcttggtatg caggttaaag
     1321 tgctggcgct ggcaccagat gccaccgacg tcgcgatggc gctattctcc gggatattta
     1381 atattggaat cggggcgggt gcgttggtag gtaatcaggt gagtctgcac tggtcaatgt
     1441 cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc gttaatttgg tcaatcatta
     1501 tatttcgccg ctggccagtg acactcgaag aacagacgca atagttgaaa ggcccattcg
     1561 ggcctttttt aatgatacgt tttaatgatt tccaggatgc cgttaataat aaactgcaca
     1621 cccatacata ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc acccttgccc
     1681 accagccgca taattgcgcc ggagctgcgt aggcttcccc acaaaataac cgcaaccagg
     1741 aaaaagatca gcggcggcgc aaccatcagt acccaatcag cgaaggttga actctgacgc
     1801 actgtggacg ccgagctaat aatcatcgct atggttcccg gaccggcggt acttggcatt
     1861 gccagtggta caaaggcaat attggcactc ggttcatctt ccagctcttc cgacttgctt
     1921 ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaaa gcatccgaaa accgataaac
     1981 gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc
     2041 accagttgcc cggcgtaata cgccaccatc atgatggcaa atacgtacac cgaggccatc
     2101 aacgactgac gattacgttc ggcactgttc atgttgcctg ccagaccaag aaatagcgcg
     2161 acagttgtta atgggttagc taacggcagc aacaccacca gccccaggcc aattgcttta
     2221 aacaaatcta acattggtgg ttgttatcct gtgtatctgg gttatcagcg aaaagtataa
     2281 ggggtaaaca aggataaagt gtcactcttt agctagcctt acatcgcatt gaccaaaact
     2341 tgaaccgatt tagcaaaacg tggcatcggt caattcattc atttgactta tacttgcctg
     2401 ggcaatatta tcccctgcaa ctaattactt gccagggcaa ctaatgtgaa aagtaccagc
     2461 gatctgttca atgaaattat tccattgggt cgcttaatcc atatggttaa tcagaagaaa
     2521 gatcgtctgc ttaacgagta tctgtctccg ctggatatta ccgcggcaca gtttaaggtg
     2581 ctctgctcta tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa agtgttgtcg
     2641 gtcgacctgg gagcactcac ccgtatgctg gatcgcctgg tctgtaaagg ctgggttgaa
     2701 aggttgccga acccgaatga taagcgcggc gtactggtaa aacttaccac cagcggcgcg
     2761 gcaatatgtg aacaatgcca tcaattagtt ggccaggacc tgcaccaaga attaacaaaa
     2821 aacctgacgg cggacgaagt ggcaacactt gagcatttgc ttaagaaagt cctgccgtaa
     2881 acaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca ttcatagcat
     2941 tttggactgg atcgaggaca acctggaatc gccactgtca ctggagaaag tgtcagagcg
     3001 ttcaggttac tctaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
     3061 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
     3121 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
     3181 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
     3241 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
     3301 tcactgaggc tatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
     3361 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
     3421 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
     3481 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagttattgt
     3541 caggctctga tcacagagcc tgacatctcc gtaatatctt cctcaagtct caaatatttt
     3601 cgccattgtt ggcgatgaga tcccgatacc agtaaaacga cgctttacgt atccgttcca
     3661 gcgttccctt cccttcgttg tctttatcga cataaatcat tccgtagcgc tttttcattt
     3721 cccctgttcc ggcagaaacc aggtcaatgc agccccacgg ggtgtagcca attaagtcaa
     3781 caccatcttc aactacggct tttttcattt cccgaatatg ggaagcaaag taatcaatgc
     3841 gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc aaccgcacca aatccatttt
     3901 cgacaataaa cagcggcaac tggaaatgat cccagaacca gttgagtgaa taacgtagcc
     3961 ctgccggatc aatttgccat ccccattcgg atgtatcgat ataagggttg ctgaccaaat
     4021 ctcgtggttc aacataatcc agttgcgggt tatcgggcga aaattgtgtc gtaaaagaca
     4081 tgtagtagct aaagccgata aagtcgacac aaccactggc aagaatcgcg ttatcttctg
     4141 gtgtgatatc gaggttgaat cctttcctgg caaagtaatt cagcatatgt tgcgcataat
     4201 atccacgagc atgaacatca gtaaaccagt aacgacgatg cattgctttc gtggccatca
     4261 tcatatcgtt gggtgcacac gtcagaggat agatgggaca catggcgatc atacagccaa
     4321 tgttaaattc aggattgatc gattttccag tctgtaccgc cagggcactg gcaactaact
     4381 cgtaatgtac cgcctggtac attatttgtt cgcgctcatt gatatcttcc tctggcgaat
     4441 acaagatacc ggaattagta aatggacaca ggctttcgct gaaattcacc tgattattga
     4501 tttcgttaaa cgtcatccag tactttactt tttctttata gcgcgtgaag accgttgatg
     4561 cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca gccaccatat tttgtcacca
     4621 gatgataagg catctcaaaa tgtgaaagcg tcaccacagg ttccattccc tgcttaaggc
     4681 attcatcgaa cagatcatca taaaattgta aaccctcttc attcggctcc tgttcgtcac
     4741 cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa gcatttgaat cccatctcgg
     4801 caaataactg aatatctgtt ttatagcgat gataaaaatc aattgcttca tgattgggat
     4861 aattaagccc gtcgataacg ccttctgtca cttcacgcgg caccccgtga gcgccagcag
     4921 tcatcacatc agcgatgctg atgccttttc ctccttcatt ccagccacct tccaactgat
     4981 gcgcggctac cgcaccgccc cataaaaaac cttttttaaa tcctgacata cacaactcct
     5041 taactaaaat agattaccac cagacttccg cctggatgcc ggccataaac tcgtctttgc
     5101 tgttctcatt aaacttaaaa ttcgataatt cgttatccag aatattgata tacgttccat
     5161 agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc cactttcaat gcatgccaga
     5221 gtgtcgtttt ataagccgat tcatttagtg ttacaccctg ctgagttttg ttctgttgct
     5281 taaaccagcc taattcaagc cccgtctgat tccatgtatt ccagatccag gccggtctta
     5341 ttacagtgcg aatactgtca aaatcactat gagcaccact ttcataacta taaacatctt
     5401 cgccgtgaga atagacaagc gcgttagcca taataatatt gtcagtaaga tacatctcgc
     5461 cctgagagat taaacgccag gcgatcccat tggtatgatc accatagtaa tagcgatcat
     5521 gcgccatcgt attactggca tctgagaaac tggcaaaact gctggcatag gaattattgg
     5581 caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag gttttgccgt aaaacagaag
     5641 tcagcatcca gctatctttc atttcaaaat aactgttgtc attttcgtta tcttgttgtt
     5701 gatccgtt
//
LOCUS       DAFAVF010000220         5630 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFAVF010000220 DAFAVF010000000
VERSION     DAFAVF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.222"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            224..1081
                     /locus_tag="LGK42_004178"
     CDS             224..1081
                     /locus_tag="LGK42_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660350.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRYEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(1203..5456)
                     /gene="fdeC"
                     /locus_tag="LGK42_004179"
     CDS             complement(1203..5456)
                     /gene="fdeC"
                     /locus_tag="LGK42_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBK2660351.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISNIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTIVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLAVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKIIRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
BASE COUNT         1324 a         1508 c         1296 g         1502 t
ORIGIN      
        1 caatgagtct acgttaaaac gtaacctcaa attagtacgt ggattttgat atcacttatg
       61 caaaaaaatc attaataatg caggactgaa acctctctat tttcggggac aacgaagcag
      121 acgctaccag tgcttttgcc ttcgcccttg ctgtttttga tacacttagg gcccagggca
      181 taacgaaaat gtgcgatatg acaaagaatt aacggagaat gtgatgatca ggcagaagat
      241 tctacagcag ctcctggagt ggattgagtg caatcttgag caccctattt caatcgaaga
      301 tatcgcacag aaatctggct acagcagacg caacatccag cttctgttcc gaaatttcat
      361 gcatgtgcct ttgggagaat acattcgcaa acgaaggctt tgccgtgccg ctattcttgt
      421 ccggctcacc gcgaaatcta tgcttgatat tgcactctct ttgcattttg attcacagca
      481 gtcattcagc cgcgaattta aaaagttatt cggctgctct ccccgtgaat accgccaccg
      541 tgattattgg gatctcgcaa atatcttccc ttctttttta atacgtcaac agcaaaagac
      601 ggagtgtaga ttagtcaatt ttcctgagac acctattttt ggcaactcat ttaaatatga
      661 cattgaagtg tcgaataaat caccggatga agaagtcaaa ctgcgacgtc atcatttagc
      721 cagatgtatg aataatttta agacggatat ctatttcgtc tccacgtttg aaccgtcaac
      781 aaaatcggtc gatttgctca cagttgaaac ttttgccggt acggtatgta aacaaaccga
      841 ttctgagatg gcgaaagagt ggacaataaa ccgaggactg tatgcatctt tccgctatga
      901 aggtgagtgg gagcgctatc ctgaatggtc gcgaaacctc tatctgatgg aattacctgc
      961 cagggggtta gccagagtga acggcagtga tattgagcga ttttattaca atgaagattt
     1021 cgtagaaaag gatagcaatg atgttgtttg cgaaattttt atccccgttc gtccggttta
     1081 gctgtcgcta tatctaaatg aacttataac gagactactg tcaggcaatt caataaataa
     1141 tggcgttgaa tatgtcaacg ccattattgt ttattagaat gctattttca tattcttaaa
     1201 aattatttca tcgcctcctc gccctttgcc gtatcggcat ccaccgcggt aatggtcacc
     1261 gtcactggct tcatttcgta cccgccatag gtcagggcgt taaatgtgaa tgtatagacg
     1321 cctggcttgc tggtactaaa tgtacgtatg attttcccgc tcacgaaatg gtcatttttc
     1381 gacggcagga actgataatc cttctccgtc accccttccg gcgtctgaat gtcgacccac
     1441 atattcccgc caacggggtt atttgccgac atcagggtca ccgccagctt cgcagtcgat
     1501 ttcccatccg ccactagaga ttgcatatca gatgatgcgg tgagtaccgc gtttttatca
     1561 tccgcaacga atttagattc ggcagttttc gttgatacct gaccgttggt ctgactcact
     1621 tgcgccttga gtgtgtatgt cgccgcaaca atagtggtag cggtgaaaat agcctgccct
     1681 tgctcattcg ttgtcgcagt gccattcggt tccaggttta acgaagccgg gctggcagtc
     1741 agcgtcactt cgctatcttt caccaaattg ttattggcat cagtcaccgt aactttatac
     1801 gtttgtttgg ctttgccgtc cgccagcgca tagttgccaa tcacctcaat attggagatg
     1861 gttgccgtag tcttatccgc cacaaacgtt acagactgcg cattcgaagg ctggtcattt
     1921 cctgccgacg ccgtgatctc ataggttccg gcagtcactg aaaccacctg caactctgct
     1981 ttcccctcat cgttggcttt cacccagaca ttatcgcctg taagcggctt gacaccccgg
     2041 ggcagattaa aggtcaccag gctccctgga acagggttac catttttatc ctcaacggtc
     2101 gccgtcagcg taaaggcgtc tttgccgttt gccacttttt gaacggcggt gtctacctgc
     2161 aggtttgcct gaccggtgct ggcatccgcg ttaaatttca ccgtgaccgt cttctgagca
     2221 ccattcaccg aagcggaaat attgtgttct cccgcaaccg ttgacataag ctcaatgtcc
     2281 actttccctg ccgcattggt tgttactgta ttccccgtct tgctggtcac accctgcggt
     2341 aaagtcagcg taacttcttg cccctgcaac gggttaccat agctgtccac gacggtcagg
     2401 gttatctggt tagcagactg tccattagcc agttggttat taaccttcac tgtcatatca
     2461 ccaatctcag ccttagatgc gtcacctgca acgttcagca ccagtggctg agcaacggca
     2521 ttttggccgt tcactcgcgg catcacagac aactgacccg cggcagatcc cagcgttaag
     2581 gtcgacacgt agaccccatt acctttctct gtccagcttc ctgctgaagg acgctcactc
     2641 cccgtgctgg cggcaccgct aaacactggt gcatctggct tcagcccggt gaccgggttc
     2701 ccgtacgcat ccttcacggt gaaggtgagt tccgtcgtcg ttttgacggt cggagcctta
     2761 ttgtccgcaa caagcgtaga gttcgctgat gacatctctc cggcgatgac cgtcaactgc
     2821 gcggcttcgg tggctgctgg ctggccgttg aagagcggca tgacacgaag ctcgcccgtc
     2881 tttccgcctg tagtcaacgt agcaacatag gaaccgtcac ctttttcggt ccaactggaa
     2941 acggtcgccc cttcagaggc ggtccccgtc aaacttgccg acaacgaaag accactgata
     3001 gcgttgccat gcgcatcttt cgccaccagc gtcacggttg tgctttcgcc ggcttttacg
     3061 tgatcttctg cgacagagac tttcgactgg tttgaagata acgcatcagc caccacggtt
     3121 acttttgccg catttgctgc cgcatcctgt ccatttagct tcggcataac ttctaattca
     3181 cccgccgtag agccgagagt aatttgcgca gtccacgtcc catcaccatt atttgtccag
     3241 cccgatgccg tagaacctac agcagcggca cccgctaatg acggcgcttc cggcgtgagg
     3301 ctggtcacag ggttgtcata ggcatctttt gccgtccaga ttgccgtaac tgtaccccca
     3361 accaccggtt tatcaggatt cagggtgatg gacgaatgtg ctgcatcaag cggcccggca
     3421 acaaacttca gcgtttgttg taatgtgccc agttgatact gctcgctgaa ggcacgtaca
     3481 acaacgtttc cggcacgggt cgaagaaacc gtggcgctgt aaacccctgg ttttatttcc
     3541 gaaatggcac caacggtaac accattagtg tcttgcggaa caagtcgcag gcggctggct
     3601 tctcccgtca ccggattacc ctcggagtcc accgcagtca acgtcaacgt gtaggcttgc
     3661 tgaccatcag caaccacgtc acctgacggc tcgttagcgc tcagggtgga gtttgccaca
     3721 tccatcatcg tggcccgcag ttctgcagtg acggttttgc tcatgccatc aacgctaaca
     3781 gtaatcgttg cctcacctga ctgcgttccg gtagtaaaga cagactgata caccccggct
     3841 tcagtttcgg tgaactcacc cagtgttggc tttgcctgtg atttagtggc cttcagggta
     3901 cgagtcacaa tatttccagc cggtttgaaa gttagttcag tcttgatctg atctttcatg
     3961 cccgtgactg gctggccctc ggcgtcgcgc agagacagca ccagcggtct ttgctcatta
     4021 ccgttagcaa gcatttgaat acggctctga ccgtcaagcg ttaacgccgt gcgatcggcg
     4081 ctcatacctg ctccggtaat gaccacctct gtctgcacgc gttttgaggc attgcctttg
     4141 ttatcgtagg caaccgcaga aatcgcataa taattgtctt tacctggacg ataagccggg
     4201 agcgttactt gccactgact accctgaccg gtaattttgc caccttcagc cagtaatgac
     4261 ggcgcttccc actgcacatt tttcagtccg tgagttgctt tgctgaccac aagccccagg
     4321 gaaagtgtct gaccgccctt accttcaata cgctcaggca gagcaatacg gatcacttca
     4381 gatttgcggt actcaagaac gatgttgtta ttacgctcaa ccaggtcata gcggctgcct
     4441 gccagcattc gacgctcgcg aatgctgtct gtatcgagtt gtttctccag aggttcgcca
     4501 atccgataat taacttccag gccaaagcga gtgtcattct caccgctctt gccctgctta
     4561 tgcccggcgc tcagtgtcag aagaggcact ggcgtatagg tcacctcggc agaaatagca
     4621 tgcgggtctt tctggcgctt atctttacca aacagcccga cttcatcgcc ataatactgt
     4681 tcatacatca ggcttgcgcc aagctgcggc caggcaggta aatagccctc tgcgcggata
     4741 tcccaaccat tcgccgggcg ttcctgataa tcctcaatat ccggcgattt tttccagcca
     4801 gaagcccgga tataaccatt ggcgctcagt ttcagataat cgcgccagta ttccgcacca
     4861 acaccaatgc gggtatgact acgggataaa tcatggtcga taaaggtgtt cacccccgcc
     4921 atccagtcat ttcctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg
     4981 tcggtacgat gtattgctcc ctgagtgaac aacatatttg ttggcgtatc ataaatcgga
     5041 taaagcattt ccagcgaaga atccttcagc gagaaatctt tatcgacatt cagtttgacg
     5101 cgcgcagtac catatttccc gagccactcc tgtatttcct ggttagcttt agctgtggcc
     5161 attccggtaa taaagttacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg
     5221 gcatttgcgg caaacgacgc gacatttttc tccacgttat tatcagcagt taccgtagta
     5281 tttcccatgc tcaaccgtgg ctgaaccgca tgctgcgcac gtgccgccat tactggggta
     5341 aaggtgacag cgagtggaaa aagaacctga acagagatat ttgcccacgc cacgcagcgg
     5401 gccagaactg aataacgaaa tcgtggttgt ttatgacctg ttttataacg cgacattgag
     5461 ataatttcct gatgaacgaa ttaacgaaca taggcagaca caaataatgg cgtctgcttg
     5521 tcagaaaaac cggggatttc ccgatttcct tttccgcaga aaaggaaata cctttatgta
     5581 agaaagtgca ccgccaccta atatatccag tgtctgtagc cactcatcag
//
LOCUS       DAFAVF010000221         5628 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFAVF010000221 DAFAVF010000000
VERSION     DAFAVF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.223"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..301)
                     /locus_tag="LGK42_004180"
     CDS             complement(<1..301)
                     /locus_tag="LGK42_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2660352.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVM"
     gene            complement(372..3785)
                     /locus_tag="LGK42_004181"
     CDS             complement(372..3785)
                     /locus_tag="LGK42_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2660353.1"
                     /translation="MGKGSSKGHTPREAKDNLKSSQMLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTIGKS
                     TFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTEDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPVFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCTIKGMLEANQVRGDFVKTVSKSFPKKAGTWGNTETPDGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGDNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(3846..4478)
                     /locus_tag="LGK42_004182"
     CDS             complement(3846..4478)
                     /locus_tag="LGK42_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2660354.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRISGRDVSTSGLTAQLHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARIPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4415..5158)
                     /locus_tag="LGK42_004183"
     CDS             complement(4415..5158)
                     /locus_tag="LGK42_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660355.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPGVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSYLPWLLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPNFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            complement(5164..>5628)
                     /locus_tag="LGK42_004184"
     CDS             complement(5164..>5628)
                     /locus_tag="LGK42_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBK2660356.1"
                     /translation="LTVSNLYGMVTGMAEDLQSLVGGTVVRRKVYARFLDAVNFVNGN
                     SDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYRGD
                     ECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT         1165 a         1764 c         1414 g         1285 t
ORIGIN      
        1 ccatcacgct gaaccagcgg ttacgcacgg aatcttcatg ccagcgggta tcgctgtaac
       61 gggtaagctg gcgattcttg tctcctgcat agctgaatga cgtcaccatc cccagtgtgt
      121 ccgtaaactc ataacggtat ttcacgttaa tcccgttcag atcatcgctg ccgggaacgt
      181 tcgtcgaggc atgaagatac cccgcgctca gcgtggactg atgttcagac gcccatgcag
      241 gcgcaccgga tacggccaga caaatggctg cggacaaaat ggctgcacaa actttacgca
      301 taattacctc tcgcttttct gcaataaaaa aggcgtcatt cctgacgccc tttattgggg
      361 ttataacaat ttcaacgaat actgatgccg gaagcagcct ttttggtcac aatcaccgta
      421 cagtcggtga tattgcctgc cccctgattg cctttctgga aaatcttaaa ctccagagtg
      481 acgcttcccc tgccactcgg catatcaata accgcactgt aactaccggg aatggctcct
      541 ttagtttctc tggatgcgat taatacgccg tttttgcgaa cttcaaaacc ataccccgtg
      601 taccttgtac ctcccgggtt atcaccactt cccggatcgt catacgctat accgttaaag
      661 ataatgggcg gaataatgat ttgacggtca aagttatgat catcgctgat ggtgactgta
      721 accgtcccgt ctggtgtttc cgtgttaccc cacgtaccag cctttttcgg gaatgatttg
      781 gatactgttt taacaaaatc acctctgacc tgattcgcct ccagcatgcc cttaatcgta
      841 cagttttcat ttatcgtgac gttgttgagc gtcccggagt tcgcattcac actgccactg
      901 atatccgcat ttttagcggt cagctttccg tccggtgtca gggaaaatac cggtggattt
      961 ccaccactgg taatggtggg ggccgtcagg cgtttgagga acacgtcgtt catgaatatc
     1021 tggttgccct gcgccacaaa catcggcgtt tcattcccgt ttgccgggtc aataaacgcg
     1081 atacgattgg cggcaaccag aaactggctc agtttgcctt cctctgtatc ctccatgctg
     1141 aggccaagcc ccgcgacata atgcttaccg tcttcggtct gctcaatttt gacgccccac
     1201 atggcattcc atttatcgtt ggcgtccttc cactctttcg aaaactcctc cagtttgctg
     1261 gcgttatcct ccgtcagctc gactttttcc agcagctcct tgccgagatg ggattcggtt
     1321 atctggcctt tgaaaaaatc caggtaacct tccgcatcat cgctcgcccg accgacagcc
     1381 tccacgaatg tcgatttgcc gatggtgttc acactgcgga tataaaagta ataatcatgg
     1441 cccggtttga tattgatact ggcggctatc cagtacagcc ccgtgccaag gtagcgggct
     1501 gtggtttcaa cctgcctgat atcggtaatc cgcgtttccg agaaccagaa ctcaaactgt
     1561 accgtcggat cataaaccgc aagatgcggc gtggcggtta tctgaaaata gcccggcgtc
     1621 agctcaatcc tcgacggtgc tgccggtgcg gcaatccgga acgataccga cgccggatcg
     1681 ccctgctgcc cccacgcatt taccgcccgg actgtcagcc tgtagtttcc tggcgccagt
     1741 tgcctgaagc ggtatgtggt ttccgtcgtc cgggctgtgc tgaccagccg ctcactgccg
     1801 tcatccgctg ttacggtcag acgaagcata aagctcacgc ccttcaccac cttcggcgtg
     1861 tcccagcgcg ccagcacctg atattccccg ctgtctgcgg tgacttcggc agtcaggtgc
     1921 tgcaccgctg gcggcgtgac accattcacc gtgccgctct ggtcgccgtc aaagtgcgcc
     1981 ccgttatcca cgatggcttc tttttccggt acatgctgca cggcggtgat ggcatacgtg
     2041 ccgtcgtcgt tctcacggat actcacgcag cggaacaggc gctggcgcag cgtcggcagc
     2101 ttcagccccc acacgctgta ttcagcaacg ccgtcaggaa cacggctcac tttcaccttc
     2161 acgccgtcgg tgacggactg aacctccacg ctgaccggat tgccacttcc gtcaaccagg
     2221 cttatcagcg tggtaccgga ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg
     2281 gtctggctgt ttaccgccag cacgcgcccg ccgatgctga taccggcata gtcatcatca
     2341 cagatttcaa ttacatcgcc cggcacatgg cgaagccctt ctgcgcccac gctgaagtcc
     2401 acggtctgcg tttccagcag ttctgtttta atcagccaca gcccggcgcg gtgtgcctgc
     2461 ccccggctgg tacagccaaa agcatccatc ttcgtgacgt tacgaccgta acgggcaatg
     2521 gcctgcgtat cctccacaag ctctgtcgcc gtctcccagc cgttgttcgg gtcaatccag
     2581 ttcacctcaa cggcattatg gcggtctttc agggcgctga agctgtagcg gaacggcgcg
     2641 ccatcatccg gcatcaccac attactgcgg ttataggtcc acaccttatc cgacggtcgg
     2701 tcctgcacga acgtcagcgt ctgcccgttc cataccggca tacagcgcat cgccgagcag
     2761 aaatcactga gcacatccca cgccttgcgc tgtgtggtca ggtacgcatt acaggtgatg
     2821 cgcggctccg tgccgccaaa gccgtccggc actgactggt cgcagtactg gccgatgaca
     2881 tacagcgccc atttgtccac atccgccgca ccaagacgtt tccccatgcc gtagcgtgga
     2941 tgggtcagca tatcccacag acaccaggcc atgttattgc tgtatgctgg cttaaacgtt
     3001 ccgtcccaga taccgctgta ttgccgcgtc tgcgggttat agttcgacgg cacctgcaga
     3061 atacgcccgc gcagatgata attacggctc acctgctggc tgccgaactg ctccgaatcc
     3121 acctgtacgc cgaccagtgc cgtgttcggg tagcactgtt tcacatcgat gatttcggtg
     3181 tatgacgacc agagcgtttt gttctgcagc tggtctgtgg tgctgtccgg cgtcatcctg
     3241 cgcatccgga tattgaacgg gcgcggcggc aggttaccca ccaccaccga tgccagatac
     3301 tgtgaggtgg ttttgccttt aatggtgatg tctttttccg tcacccagcc accattacgc
     3361 tgtatctgaa ccagcaggcg gacttccgac ggattccggt ccccctttga ggtggtttcc
     3421 accagtgcct gcacaccgaa ggtaaagcgc agacggtcaa tgtttgcaga cgtgatggtc
     3481 cgggtaatcg gcgtgtcgta cttcacttcc gtacccagca ccgtctcgga gccggaggat
     3541 tcaaatccct ccggcggtgt ctgctcctgc tcacctgccc ggaaaaccac cgtgacaccg
     3601 gagatattgg tattcccctc actgtccagc actggcgtac tgttcagcag cacacttttt
     3661 aatccgtcca ccggaccttc aaccggccct tcactgatgg cgtctatcac gctcagcatc
     3721 tgggatgatt tcaggttgtc cttcgcttcg cgcggggtat gccccttact gctgccttta
     3781 cccattcctc acgctccaga aacgacaaaa ccgccctgag gcggtttcac ataaaacgtt
     3841 tttcatcaac gaccaatcac cacaacctga ccaccgtccc cctcgtctgc cgtgctgatc
     3901 tcctgagaaa ccacgcgtga ccccacgcgc atttcaccgt acagaaccgg cagaacattg
     3961 ccctgggcaa ccatgttatc cagtgaggag aaataggtgt tctgtttgcc gttatccgtt
     4021 gtctgtgtac ggggaattct ggctttcggt gccagcatct gcgccacacc accgagcacc
     4081 atactggcac cgagagaaaa caggatgccg gtcataccac cggccccaat ggctgccccc
     4141 catgctgcaa gggtggcccc ggcggtaaag aatgatccgg caatggcggc agctcccagg
     4201 acaatctgga atacgccacc tgacttggcc ccggcgactc tgggaacaat atgaatcaca
     4261 gcgccgtcag gcagaacctc atgtaactgc gccgtcaatc cggacgtgct gacgtcccgc
     4321 ccggaaatcc gtacctgata ccagccgtcg ctcagtttct gacgaaacgc cgggagctgt
     4381 gtggccagcg cccggatggc ttcagccccc gttttcacac gaaggtcgat gcggcggcca
     4441 aatcgttgta aatccccgta aaggcagatg cgcgccatgc ccggtgacgc cagagggagt
     4501 gtgtgcgtcg ctgccatttg tcggtatacc tctctcgttt gctcagttgt tcaggaatat
     4561 ggtgcagcag ctcgccgtca ccacagtaaa tggcggcatg attcggcacc gatgaaccaa
     4621 aacagcacag cagcacatcg cccggttgtg ctgatgacaa cggcacctga tacagccctg
     4681 tggcctccag attatccaga tagagattct ggccgtgacg ccaccagtca tcctcgcgat
     4741 gaaaattcgg catctcaatc cccgccagat gataagcatc ccggaacagc gtgtaacagt
     4801 ccgtcacccc gtgctcaaag cgccgcccgg tgagatgcgg cacacagcgg aacttatgaa
     4861 tcgccccccg gcagaccagc aaccacggca aataactctg cacctgcagc cgccggtcag
     4921 cctcactcag ccagggcaga ccaccggggt ggctgtggac cagcgccaca atctcaccct
     4981 gcatttctgc ctgcagccag tcctccggag ccatccggaa ataatcctcc ggctcaccgg
     5041 aaatattcac gccgggaaaa tatctttccc cctccggcgc tctcaccacg aagccgcacg
     5101 actccgctgg cgcacatcgc cgggcgtgcg ccagaatcgc tgattctgtc tctgtcatgg
     5161 gatttactgc gaaagtttgt taatggaaag gaagccgcca aagttgccga cgttattgcg
     5221 aaacttacag ccgctcaggc atttgctgca tttatccttc gtgatatcgg acgttggctg
     5281 gtcatattca tccgcgaccg ccggaccgtg ataaccgcac tcatcgccgc gataggtcca
     5341 ggtgcaggtg ttggccagca tgatacgtcc cggaaaaaca gcgccatctg tttccgtcgg
     5401 cgtggacagt acaaaggagg cactcaccgc gctcagttcg ctgcactgct cgatgcgcca
     5461 gcggctgatc acctcctgct ccggatcggc gtcactgttt ccgttgacga agttcaccgc
     5521 atccagaaaa cgggcgtaaa ccttacgccg gaccaccgtt ccgccgacca gactctgcag
     5581 atcttccgcc atcccggtga ccataccgta caggttagaa accgtcag
//
LOCUS       DAFAVF010000222         5505 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFAVF010000222 DAFAVF010000000
VERSION     DAFAVF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.224"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            72..986
                     /locus_tag="LGK42_004185"
     CDS             72..986
                     /locus_tag="LGK42_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBK2660357.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            1058..2305
                     /locus_tag="LGK42_004186"
     CDS             1058..2305
                     /locus_tag="LGK42_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBK2660358.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2835..3035)
                     /gene="torI"
                     /locus_tag="LGK42_004187"
     CDS             complement(2835..3035)
                     /gene="torI"
                     /locus_tag="LGK42_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBK2660359.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(3093..3260)
                     /locus_tag="LGK42_004188"
                     /pseudo
     CDS             complement(3093..3260)
                     /locus_tag="LGK42_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3349..3630)
                     /locus_tag="LGK42_004189"
     CDS             complement(3349..3630)
                     /locus_tag="LGK42_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660360.1"
                     /translation="MSINELESEQKDWALSMLCRSGVLSPCRHHEGIYVDEGIDIKSA
                     YKYSMKVYKSNEDKSPFCNVREMTDTVQNYYHEYGGNDTCPLCTKHIDD"
     gene            complement(3833..4384)
                     /locus_tag="LGK42_004190"
     CDS             complement(3833..4384)
                     /locus_tag="LGK42_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBK2660361.1"
                     /translation="MSEINSQALRAKAEKATCGEWSLEYGESRFDGDDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEEIALTVGKLRVELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVEVMRIAGDA
                     QLSTPCTDSIINSIATGIRIKGE"
     gene            complement(4381..4602)
                     /locus_tag="LGK42_004191"
     CDS             complement(4381..4602)
                     /locus_tag="LGK42_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBK2660362.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            4971..>5505
                     /locus_tag="LGK42_004192"
     CDS             4971..>5505
                     /locus_tag="LGK42_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660363.1"
                     /translation="MVNQQQQQINENLKIEAIRWYQKLQEITYLEAGQHMRALNQLMW
                     QIPSFVIAVNGGLWYATTLANESSLWIIFAVLALFDFTTMITLYRLRSLIGSKIALQK
                     NIEDPSKNYILNEITRDAGFKLPSSYDNKETGYIVVSCWAVMLIVCFFVNIAGVYHPN
                     LFSKDISHNTYKATIKNI"
BASE COUNT         1390 a         1171 c         1261 g         1683 t
ORIGIN      
        1 taaatccatg acgattttgc gaaaaagagg tttatcacta tgcgtaactc agatgaattt
       61 aagggaaaaa aatgtcagcc aaagtatggg ttttagggga tgcggtcgta gatctcttgc
      121 cagaatcaga cgggcgccta ctgccttgtc ctggcggcgc gccagctaac gttgcggtgg
      181 gaatcgccag attaggcgga acaagtgggt ttataggtcg ggtgggggat gatccttttg
      241 gtgcgttaat gcaaagaacg ctgctaactg agggagtcga tatcacgtat ctgaagcaag
      301 atgaatggca ccggacatcc acggtgcttg tcgatctgaa cgatcaaggg gaacgttcat
      361 ttacgtttat ggtccgcccc agtgccgatc tttttttaga gacgacagac ttgccctgct
      421 ggcgacatgg cgaatggtta catctctgtt caattgcgtt gtctgccgag ccttcgcgta
      481 ccagcgcatt tactgcgatg acggcgatcc ggcatgccgg aggttttgtc agcttcgatc
      541 ctaatattcg tgaagatcta tggcaagacg agcatttgct ccgcttgtgt ttgcggcagg
      601 cgctacaact ggcggatgtc gtcaagctct cggaagaaga atggcgactt atcagtggaa
      661 aaacacagaa cgatcgggat atatgcgccc tggcaaaaga gtatgagatc gccatgctgt
      721 tggtgactaa aggtgcagaa ggtgtggtgg tctgttatcg aggacaagtt caccattttg
      781 ctggaatgtc tgtgaattgt gtcgatagca cgggggcggg agatgcgttc gttgccgggt
      841 tactcacagg tctgtcctct acgggattat ctacagatga gagagaaatg cgacgaatta
      901 tcgatctcgc tcaacgttgc ggagcgcttg cagtaacggc gaaaggggca atgacagcgc
      961 tgccatgtcg acaagaactg gaatagtgag aagtaaacgg cgaagtcgct cttatctcta
     1021 aataggacgt gaatttttta acgacaggca ggtaattatg gcactgaata ttccattcag
     1081 aaatgcgtac tatcgttttg catccagtta ctcatttctc ttttttattt cctggtcgct
     1141 gtggtggtcg ttatacgcta tttggctgaa aggacatcta gggttgacag ggacggaatt
     1201 aggtacactt tattcggtca accagtttac cagcattcta tttatgatgt tctacggcat
     1261 cgttcaggat aaactcggtc tgaagaaacc gctcatctgg tgtatgagtt tcatcctggt
     1321 cttgaccgga ccgtttatga tttacgttta tgaaccgtta ctgcaaagca atttttctgt
     1381 aggtctaatt ctgggggcgc tattttttgg cttggggtat ctggcgggat gcggtttgct
     1441 tgatagcttc accgaaaaaa tggcgcgaaa ttttcatttc gaatatggaa cagcgcgcgc
     1501 ctggggatct tttggctatg ctattggcgc gttctttgcc ggcatatttt ttagtatcag
     1561 tccccatatc aacttctggt tggtctcgct atttggcgct gtatttatga tgatcaacat
     1621 gcgttttaaa gataaggatc accagtgcgt agcggcagat gcgggagggg taaaaaaaga
     1681 ggattttatc gcagttttca aggatcgaaa cttctgggtt ttcgtcatat ttattgtggg
     1741 gacgtggtct ttctataaca tttttgatca acaacttttt cctgtctttt atgcaggttt
     1801 attcgaatca cacgatgtag gaacgcgcct gtatggttat ctcaactcat tccaggtggt
     1861 actcgaagcg ctgtgcatgg cgattattcc tttctttgtg aatcgggtag ggccaaaaaa
     1921 tgcattactt atcggagttg tgattatggc gttgcgtatc ctttcctgcg cgctgttcgt
     1981 taacccctgg attatttcat tagtgaagtt gttacatgcc attgaggttc cactttgtgt
     2041 catatccgtc ttcaaataca gcgtggcaaa ctttgataag cgcctgtcgt cgacgatctt
     2101 tctgattggt tttcaaattg ccagttcgct tgggattgtg ctgctttcaa cgccgactgg
     2161 gatactcttt gaccacgcag gctaccagac agttttcttc gcaatttcgg gtattgtctg
     2221 cctgatgttg ctatttggca ttttcttctt gagtaaaaaa cgcgagcaaa tagttatgga
     2281 aacgcctgta ccttcagcaa tatagacgta aactttttcc ggttgttgtc gatagctcta
     2341 tatccctcaa ccggaaaata ataatagtaa aatgcttagc cctgctaata atcgcctaat
     2401 ccaaacgcct cattcatgtt ctggtacagt cgctcaaatg tacttcagat gcgcggttcg
     2461 ctgatttcca ggacattgtc gtcattcagt gacctgtccc gtgtatcacg gtcctgcgaa
     2521 ttcatcaagg aatgcattgc ggagtgaagt atcgagtcac gccatatttc gctatcagga
     2581 ttctgtgtga tggttacatc gcccggccca gggctgttta gtcatcagcg ctttctgaca
     2641 gtgctgagat ttcaacctgt tgcagtaaaa atgagtagat ataaggcaag tgtgctgcca
     2701 aacccatctt ttacggggtg aaggtagatt tcgtttgaag ggtatctggt gtcccctgca
     2761 gacatctact tcaagcggca ggggattgat tggaatggtg ttttttagat gtgaaaaata
     2821 ttttacccgc tattttaccc attggcgcgg cttaagagct tatttttgaa ttcacaatgg
     2881 tcacgatata accatcttgc tcgcccgtgg ataactttgg cttttggcag gtcgccggac
     2941 ttaatccggt catagatgaa ggttttaccg aagccagtat cggccatgat gaatttcaaa
     3001 tcaaccagtg aatcaggctg tagttcgtgt tgcatgagtg ctatctccga atagggaatc
     3061 gaacctgcaa atcaggtaat aaaaaaccgc catcaggcgg cttggtgttc tttcagttct
     3121 tcaatacaaa tattggttac gtctgcatgt gctatctgcg cccatatcat ccagtggtca
     3181 tagcagtcgt tgatgttctc tgcttcgata actctgttga atggttctcc attctattca
     3241 cttgtgactc ggaagtgcat ttatcatctc cataaaacaa aactcgccgt agtgagttca
     3301 gataaaaaaa atccccgcga gtgcgaggat tgttatgtaa tattgggttt aatcatctat
     3361 atgttttgta cagagagggc aagtatcgtt tccaccgtac tcgtgataat aattttgcac
     3421 ggtatcagtc atttctcgca cattgcagaa tggggatttg tcttcattag acttataaac
     3481 cttcatggaa tatttgtatg ccgactttat atctatacct tcatctacat aaataccttc
     3541 gtgatgtctg catggagaca agacaccgga tctgcacaac attgataacg cccaatcttt
     3601 ttgctcagac tctaactcat tgatactcat ttataaactc cttgcaatgt atgtcgtttc
     3661 agctaaacgg tatcagcaat gtttatgtaa agaaacagta aaataatact caacccgatg
     3721 tttgagtacg gtcatcatct gacactacag actctggcat cgctgtgaag acgacgcgaa
     3781 attcagcatt ttcacaagcg ttatctttta caaacccgta ttcctgctca tatcactctc
     3841 ctttgatgcg aatgcctgtt gcaatgctgt ttatgatgct gtcagtgcat ggggtagaaa
     3901 gctgggcatc tccagcaatt ctcatgacct caacatctgc atatcgaata ccgaggtgta
     3961 tcagaccggc tatacctgac ttaagccgag cattttccat aaacagatcc tttgcccgct
     4021 gtttttctgc ctcaagctca acgcgcaact tccctaccgt taacgcaatc tcctcgttct
     4081 cctggtcgcg gcgtttgatg tattgctggt ttctttccag ctcatccagc agcgccaaga
     4141 cggtagcggg attggctgcg gcgatgaatt cagcattggc ctgctgttcc atttggaaat
     4201 cttcatcgaa accgctttct ggatgcgctc cttcaattct gcaaatagga atatatccag
     4261 caacttcacg atgaattagc gcatcatcac catcaaatcg gctctctcca tattcgagcg
     4321 accactcacc acacgttgct ttttctgcct tggcccgcag tgcctgagag ttaatttcgc
     4381 tcacttcgaa cctctctgtt tactgataag ctccagatct tcctggcaac ttgcacaagt
     4441 ccgacaaccc tgaacggcca ggcgtcttcg ctcatctatg ggatcgccac actcacaaca
     4501 atgagtggca gatatagcct ggtggttcag gcggcgcatt tttattgctg tattgcgctg
     4561 taattcttcg atttctgatg ctgaatcaat gatgtctgcc atctttcatt aatccctgaa
     4621 ttgttggtta atacgcttga gggtgaatgc gaataataaa aaaggagcct gtagctccct
     4681 gatgattttg cttttcatgt tcatcgctcc ttaaagatgc cgtttaacat gccgatcgcc
     4741 aggcttaaat gagtcggtgt gaatccctca gcgttaccgt ttcgcggtgc ttcttcagta
     4801 cgctacggca aatgttattg atgctcctgt ctggtgacag ccatcgggct tgtattatgt
     4861 ataagttctg ataaaagcct ccgatatatt aaatttaaat aacgaaattc atcttcttga
     4921 taagttgtga tagcatcatt aaaagtttca gttcgttgag cagggattac atggtaaatc
     4981 agcaacagca acagattaac gaaaatctaa aaattgaggc aatccgttgg tatcagaagc
     5041 tgcaggagat tacttatctt gaagcaggac aacacatgcg ggctttaaat cagctcatgt
     5101 ggcaaatccc tagtttcgta attgctgtca atggtgggtt gtggtatgca acaacactgg
     5161 caaatgaaag ttccctatgg attatctttg cagtgttagc attgtttgat ttcacaacaa
     5221 tgattacact ttatcgctta aggtctttaa ttggttcgaa aatagcacta caaaaaaaca
     5281 ttgaagatcc ttcaaaaaac tatattttaa atgaaattac tagagacgct ggatttaaat
     5341 tgccctctag ctatgataat aaggaaactg gttacatagt agtgtcgtgc tgggcagtaa
     5401 tgcttattgt ttgcttcttt gttaatattg ctggggtata tcatccaaac ttgttttcaa
     5461 aagacatttc acataataca tacaaagcaa caattaaaaa tattg
//
LOCUS       DAFAVF010000223         5380 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFAVF010000223 DAFAVF010000000
VERSION     DAFAVF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.225"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..261
                     /locus_tag="LGK42_004193"
     CDS             <1..261
                     /locus_tag="LGK42_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBK2660364.1"
                     /translation="SASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYNGPAVA
                     DEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            267..1010
                     /locus_tag="LGK42_004194"
     CDS             267..1010
                     /locus_tag="LGK42_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660365.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLACRGEIHK
                     FRCVPYLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNDQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            908..1555
                     /locus_tag="LGK42_004195"
     CDS             908..1555
                     /locus_tag="LGK42_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBK2660366.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGGEAIQAL
                     ATQLPAFRQKLNEGWYQVRIAGRDAGENELSARLNEPLENGAVIHIVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPAGWLGAAALSGMYAAGASMILGGVAQMLAPKASTVTTTSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1616..5011
                     /locus_tag="LGK42_004196"
     CDS             1616..5011
                     /locus_tag="LGK42_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBK2660367.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGNQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPATPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGSGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKGLLEKVDLTEDNASRLDQFSKEWKDAN
                     DKWNAMWGVKIEQTEDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNEVFLKRLTAPTITSGGNPPVFSLTPDGKLTAKNADISGSVNANAGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFRGAKHERENNDIYSSCRLIVKKNGAEIYNRTALDNTLVYTGVIDMPA
                     GRGHMTLEFSVSAWLVNDWYPTASISDLLVVVMKKSTAGITIS"
     gene            5080..>5380
                     /locus_tag="LGK42_004197"
     CDS             5080..>5380
                     /locus_tag="LGK42_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBK2660368.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVM"
BASE COUNT         1224 a         1344 c         1686 g         1126 t
ORIGIN      
        1 agtgcctcct ttgtgttgtc cacaccgacg gaaacggatg gtgccgtttt tccggggcgc
       61 atcatgctgg ccaacacctg cacctggacc tatcgcggtg atgagtgcgg ttataatggt
      121 ccggctgtcg cggatgaata tgaccagccg acgtccgata tcacgaagga taaatgtagc
      181 aaatgcctga gtggctgtaa gtttcgcaat aacgtcggca actttggcgg cttcctttcc
      241 attaacaaac tttcgcagta aatcccatga cagagacaga atcagcgatt ctggcgcacg
      301 cccgacgatg tgcgccagcg gagtcgtgcg gcttcgtggt gagaacgccg gagggggaaa
      361 gatattttcc ctgcgtgaat atctccggtg agccggagga gtatttccgg atgtcgccgg
      421 aggactggct gcaggcagag atgcagggtg agattgtcgc gctggtccac agccatcccg
      481 gtggtctgcc ctggctgagt gaagccgacc ggcggctaca ggtgcagagt gatttgccgt
      541 ggtggctggc ttgccgcggg gagattcata aattccgctg tgtgccgtat ctcaccgggc
      601 ggcgctttga gcacggggtg acggactgtt acacgctgtt ccgggacgct taccatctgg
      661 cggggattga gatgccggat tttcaccgcg aggatgactg gtggcgtaac gaccagaatc
      721 tctatctgga taatctggag gccacagggc tgtatcaggt gccgttgtca gcggcgcagc
      781 cgggcgatgt gctgctgtgc tgctttggtt catcggtgcc gaatcatgcc gctatttact
      841 gcggcgacag cgagctgctg caccatattc ctgaacaact gagcaaacga gagaggtata
      901 ccgacaaatg gcagcgacgc acacactccc tctggcgtca ccgggcatgg cacgcatctg
      961 cctttacggg gatttgcaac gatttggccg ccgcatcgac cttcgtgtga aaacgggggg
     1021 cgaagccatc caggcgctgg ccacacagct cccggcgttt cgtcagaaac tgaatgaggg
     1081 ctggtatcag gtgcgcattg ccgggcgtga tgcaggcgaa aacgaattat cagcccgtct
     1141 taatgagccg ctggaaaatg gtgccgtgat ccacatcgtt ccgcgtcttg cgggagcaaa
     1201 aagtggcggt gtgtttcagg cggtgctggg tgcggcgctg attgcggtgg cgtggtggaa
     1261 ccctgcaggc tggctgggag ctgcggcttt atcgggcatg tatgctgcgg gggccagtat
     1321 gatccttggc ggtgtggcgc agatgctggc accgaaagcc agtaccgtaa caacgaccag
     1381 cacggataac ggtaagcaga acacgtattt ctcgtcactg gataacatgg ttgcccaggg
     1441 caatgttctg cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tttctcagga
     1501 gatcagcacg gcagacgaag gggatggtgg tcaggttgtg gtgattggcc gctgatgcaa
     1561 aatattttat gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg
     1621 taaaggcagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca
     1681 gttactgagt gtgatcgatg ccatcagcga agggccgata gaaggtccgg tggatggatt
     1741 aaaaagcgtg ctgctgaaca gtacgccggt gctggacagt gaagggaata ccaacatcgc
     1801 cggtgtcacg gtggtattcc gggcaggtga gcaggagcag acaccgccgg agggttttga
     1861 atcatcaggt tccgagacgg tactgggtac ggaagtgaaa tacgacacgc cgatcacccg
     1921 gaccatcacg tctgcaaata tcgaccgtct gcgctttacc ttcggtgtgc aggcactggt
     1981 ggaaaccacc tcaaagggag acaggaatcc gtcggaagtt cgcctgctgg ttcagataca
     2041 gcgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacctcgca
     2101 gtatctggcc tcggtggtgg tggataacct gccgccgcgc ccgtttaata tccggatgcg
     2161 caggatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata
     2221 caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg gcgtacaggt
     2281 ggattcggag cagttcggca gccagcaggt gagccgtaat tatcatcttc gcgggcgtat
     2341 tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg
     2401 aacgtttaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca
     2461 tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggataaat gggcgctgta
     2521 tgccatcggc cagtactgcg accagtcggt gccggacggc tttggcggca cggagccgcg
     2581 catcacctgt aatgcgtacc tgaccacaca gcgcaaggcg tgggatgtgc tcagtgattt
     2641 ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga
     2701 ccgaccatca gataaggtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg
     2761 cgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgccg ttgaggtgaa
     2821 ctggattgac ccgaacaacg gctgggagac ggcaacagag cttgtggagg acacgcaggc
     2881 cattgcccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagtcgggg
     2941 gcaggcacac cgagccgggc tgtggctgat taaaacagaa ctgctggaaa cgcagaccgt
     3001 ggacttcagc gtgggtgctg aagggcttcg ccatgtaccg ggcgatgtca ttgaaatctg
     3061 cgatgatgac tatgccggta tcagcaccgg cgggcgcgtg ctggcggtga acagccagac
     3121 ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggtacca cgctgataag
     3181 cctggttgac ggaagtggta atccggtcag cgtggaggtt cagtccgtca ccgacggact
     3241 taaggtgaaa gtgaaccggg ttcctgacgg cgttgcagaa tacagtgtgt gggggctgaa
     3301 gttgccgacg ttgcgtcagc gcctgttccg ctgtgtgagt atccgtgaga acgatgacgg
     3361 cacgtatgcc atcactgccg tgcagcatgt gccggaaaaa gaagccatcg tggataacgg
     3421 ggcgcacttt gacggtaacc agagcggcac ggtgaatggt gtcacgccgc cagcagtgca
     3481 gcatctgacc gcagaagtca ccgcagacag cggggagtat caggtactgg cccgctggga
     3541 cacgccgaag gtggtgaagg gggtgagctt tatgcttcgc ctgaccgtgg cagcggatga
     3601 cggcagtgag cggctggtca gcacggcccg gacgacggaa accacatacc gcttcacgca
     3661 actggcgctg ggaaactaca ggctgacagt ccgggcggca aatgcctggg ggcagcaggg
     3721 cgatcctgca tcggtatcgt tccggatcgc cgcaccggca acgccgtcgc ggattgagct
     3781 gacgccaggc tattttcaga taaccgccac gccgcatctt gccgtttatg atccgacggt
     3841 acagtttgag ttctggttct cggaaacgcg gattaccgat atcaggcagg ttgaaaccac
     3901 agcccgctac cttggctcgg ggctgtactg gatagccgcc agtatcaata tcaaaccggg
     3961 ccatgattat tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg cattcgtgga
     4021 ggccgtcggt cgggcgagcg atgatgcgga aggttacctg gattttttca aaggcaagat
     4081 aaccgaatcc catctcggta aagggctgct ggaaaaagtc gacctgacgg aagataacgc
     4141 cagcagactg gatcagtttt cgaaagagtg gaaagacgcc aacgataaat ggaatgccat
     4201 gtggggcgtc aaaattgagc agaccgaaga cggtaagcat tatgtcgcgg gacttggcct
     4261 cagcatggag gatacagagg aaggcaaact gagccagttt ctggttgccg ctaaccgtat
     4321 cgcgtttatt gacccggcaa acgggaatga aacgccgatg tttgtggcgc agggcaacca
     4381 gatattcatg aacgaagtgt tcctgaaacg cctgacggcc cccaccatta ccagtggtgg
     4441 aaatccaccg gtattttccc tgacaccgga cggaaagctg accgctaaaa atgccgatat
     4501 cagtggcagt gtgaatgcga acgccgggac gctcaacaat gtcacgataa atgaaaactg
     4561 tcgggttctg ggaaaactgt ctgcgaacca gattgaaggc gatctcgtta aaacagtggg
     4621 caaagctttc ccccgggact cccgtgcacc ggagcggtgg ccatcaggga ccattaccgt
     4681 cagggtttat gacgatcagc cgtttgaccg gcagattgtt attcccgcgg tggcgtttcg
     4741 tggcgctaaa catgagcggg agaataacga tatttattcg tcatgccgcc tgatagtgaa
     4801 gaaaaacggt gctgaaattt ataaccgtac cgcgctggat aatacgctgg tttatacagg
     4861 tgttattgat atgcctgctg gtcgcggtca catgacgctg gagttttcgg tatcagcgtg
     4921 gctggtaaat gactggtatc ccacagccag tatcagtgat ttgctggttg tggtgatgaa
     4981 gaaatccaca gcaggtatca cgattagctg aattttcata acccatatgc gggcgccatt
     5041 tctggcgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc
     5101 attttgtccg cagccatctg tctggccgta tccggtgcgc ctgcatgggc gtctgagcag
     5161 caggccacac tgagcgcagg gtatcttcat gcccgtacga acgctcccgg cagcgataat
     5221 ctgaacggga ttaacgtgaa ataccgttat gagtttacgg acacgctggg gctgattacg
     5281 tcattcagtt atgccaacgc tgaagatgag caaaaaacgc attacagcga tacccgctgg
     5341 catgaggatt ccgtgcgtaa ccgctggttc agcgtgatgg
//
LOCUS       DAFAVF010000224         5352 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFAVF010000224 DAFAVF010000000
VERSION     DAFAVF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.226"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            58..294
                     /locus_tag="LGK42_004198"
     CDS             58..294
                     /locus_tag="LGK42_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660369.1"
                     /translation="MQMHEHAGSAPAPAALTRDREGKCMRFDAVMHAELKMAEHTEKT
                     HKKTGIQVAGLSRLHCGYWPRYAPIIPGVNLITS"
     gene            complement(567..1019)
                     /locus_tag="LGK42_004199"
     CDS             complement(567..1019)
                     /locus_tag="LGK42_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBK2660370.1"
                     /translation="MAKIHEVKLHAKYFDLVLEGKKRAEFRKNDRNYERGDTLILHEW
                     GQGVYTGRKVEARITDVTDLSDWLEDYVLLSIELLSTGAYEIVNWKELSERGLVFRIN
                     HEIMHQLGLAVMYEPETGMSGGAMVATDGAWNYSDEQMERAQQNGWLG"
     gene            complement(1019..3304)
                     /locus_tag="LGK42_004200"
     CDS             complement(1019..3304)
                     /locus_tag="LGK42_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBK2660371.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGVYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGSDASLPAPTPSEHAAAVLNLVDDGVIEWNDPAVVRALRGALKHGLRTPNRQ
                     QRNESPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMEWT"
     gene            complement(3294..3569)
                     /locus_tag="LGK42_004201"
     CDS             complement(3294..3569)
                     /locus_tag="LGK42_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBK2660372.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            complement(3566..3790)
                     /locus_tag="LGK42_004202"
     CDS             complement(3566..3790)
                     /locus_tag="LGK42_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBK2660373.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3790..4092)
                     /locus_tag="LGK42_004203"
     CDS             complement(3790..4092)
                     /locus_tag="LGK42_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBK2660374.1"
                     /translation="MSCSRSVVLLNNALKIAVMKNGDLSLIQLGIDKEKREITESVIA
                     IYQSELNLLSDVVNLLVKRAVFHKQVSSVDELTKLMTELASYCADEFKKLNDKRSW"
     gene            complement(4092..4316)
                     /locus_tag="LGK42_004204"
     CDS             complement(4092..4316)
                     /locus_tag="LGK42_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBK2660375.1"
                     /translation="MHTVSENQCGIYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4380..4880)
                     /locus_tag="LGK42_004205"
     CDS             complement(4380..4880)
                     /locus_tag="LGK42_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HBK2660376.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4877..5047)
                     /locus_tag="LGK42_004206"
     CDS             complement(4877..5047)
                     /locus_tag="LGK42_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660377.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(5058..5333)
                     /locus_tag="LGK42_004207"
     CDS             complement(5058..5333)
                     /locus_tag="LGK42_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HBK2660378.1"
                     /translation="MSKQVTLMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTD
                     PQSASGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
BASE COUNT         1292 a         1454 c         1215 g         1391 t
ORIGIN      
        1 ttttcttacg cgcagtgctt tccccgcctc gcccgcccgc ttcatggggt ggttttaatg
       61 cagatgcacg aacacgccgg aagcgcgcca gcaccagcgg cgctcacacg tgacagggaa
      121 ggaaaatgca tgcgattcga tgcagtaatg catgcagagc taaaaatggc agaacatact
      181 gaaaaaacgc ataaaaaaac cggcattcag gttgccggtt tgagtcggct tcattgtggt
      241 tactggccgc gctatgcgcc aataatacca ggagtcaatc tgataaccag ctaacgcctc
      301 gctacgctcg ttgttcaacc ccgccagccc tgaacacaag tttcacgact ggggcgttct
      361 ctatcatctg cgtggtggtg gcgtaactct tgattgaccg atatggttaa accgcccgta
      421 attatcccgg actatttcgg cacacccgac tagctcatcg ggcgtcagat tttcgttgac
      481 cataatccgc tgtaaacgct gaacaatagc catcagcttg atatttttag ttttatggtg
      541 cggtatctcg cctggtattc tgtgcattat ccaagccacc cgttttgctg tgcacgctcc
      601 atctgttcat ctgaatagtt ccatgctcca tccgtggcaa ccattgcccc gccagacatc
      661 cccgtctctg gttcatacat aacagcaagg ccgagctgat gcataatttc atgattaatt
      721 ctgaatacca ggccacgctc actaagttct ttccagttca caatctcata tgcgcctgta
      781 ctaagcagct caatacttag caagacatag tcttccagcc agtctgacag gtcagtaaca
      841 tctgttatcc gggcttcaac ctttcgcccc gtatacacac cctgccccca ttcatgcaaa
      901 atcaacgtgt ccccgcgctc ataattacgg tcatttttcc gaaactctgc gcgtttcttt
      961 ccttccagca caaggtcgaa atattttgcg tgcagcttta cctcgtgaat ttttgccatc
     1021 atgtccactc cattactgtt gagaatcccg gccactcatc agcgaccgga tacgtgaatt
     1081 ttttcccgtc ataatttacg gtcgcgccac gcgccagcgc ctcaagctcc catcgttgcg
     1141 gcctgatacc gttctgagcg agatcaacgc ggatacgggt aatttgcatt ctttccgacc
     1201 gggtcagtct ggccgatggt gcaatttcat gtggttttaa cgggctttcg tttctttgct
     1261 gacggtttgg cgttcgcagg ccgtgtttta atgctcccct gagcgccctc acgaccgccg
     1321 ggtcattcca ttcgataaca ccgtcatcaa ccagatttag cactgctgca gcgtgctcag
     1381 aaggtgtggg agccggtaac gaagcatcac taccggtgag ctttccacag ttattgacag
     1441 gactccgagg cgcggcgatg ccgcttttta aagtcaaagg ctcaacgacc ggaactttcg
     1501 gcacaatgcg ccagtccgtc gttctggtga tatgaatatg acgcgcgccg agatgcggcg
     1561 cgtaaatgcc gaccactctc tcgacttctt cctcgtactc gttaacgtca tccgacgggc
     1621 tacgggcaac cctgacagtc tgacaatcgc gcgggacatt tgccccaccc tgcgcgctga
     1681 tatacaacgc aaaatcacca ctgtctgcgg cagcgcgagc agcctcgacg cgttcgtcaa
     1741 actcatcagc aatactgacg ccgcgaggca atttgcgtag ttcacggtaa gcccccattg
     1801 tcggcagtcc aaccgtttta aattgcggga tgcgccacgt tgacgcccat gcggtaacag
     1861 ccgcggcagt atctttaagc ggcttaccgg tatcgttatc gagctgacca tccagtgcat
     1921 agccgtcgat attttttgaa atgtatttcg cgatatatcc cgcagcaccg ccccggttaa
     1981 ggtgctttgc ctgaaaacgg tttcgcgcgg ctcctctttc gtcaccatcc tctttgagcg
     2041 catagcgacg catgatttcg ataatctggt tacgctggcg tggattacaa aaaagcatca
     2101 tatgccagtg cggcgttccg tcgtggtgtg gctcgacgac acgcaaaccg tagacctgta
     2161 aatcattatc cttgaatgcc gtgcgcatca ggctccagat gcggcagaga taacgctgcg
     2221 catcctttgg attaaatgcc tcatcgttcc agccgtgatt aagctgaacg gttttacttt
     2281 cgccttttcc gacctgacgt gtcgggtgat actttgacgg cgcggtcagc gtgataaaca
     2341 tccccacatc accctctgcg gcggcgtaac gctcaatacc ggcaatggtg ttcatcagct
     2401 ccatccggcg aatttcagga ttagaaatac tgcccatcac cttactgata aggtcgatgc
     2461 gctcgccggt ttccctgttt tcaaggtcac acgatttaag aaattccaga tttgcctggc
     2521 ggcgcgcacg cacatcacga atggcgtgtt tactggcata aggagaacgg tctttattga
     2581 cctccccgac agctatcagt aacgcttcat gccagcgcat acgctggcct ttaagctgat
     2641 gagtccacca ctcatcgtta aacagacggg caatggcaga atatgcctgc ctcgtggtca
     2701 tctgcccttt acggtatttt ttccagtaga gaggggaaat attgaaagca cgtgcagcgc
     2761 cagcaacatg accatagagg tgagcctgcg cctcatccgt aaacagcgat tctttttcgc
     2821 catgcgcatc cacccaggca tcgctgagtt cctcatacat catgaaaagc tgcgatgaaa
     2881 tacgggcggc aaactttttc agctccttgt cattcattcc aggcaggcgc gcatagtggt
     2941 cacgctctgc cagaaacagc aacgacgcgt cggtgttcat ttcatggcgc tgattcacac
     3001 gctcaatgcg cggccataaa cgacgctgaa aagtggatgt gagaaaataa accccgtgca
     3061 ccgggctttt attgcgccgg atgtagtcat agcgtgaagt aaacagcgaa cgcaaaaagt
     3121 aaggcaggcg attaatcgtg gataaaacac cttgcacctg acgcatctcg tcacgtgtaa
     3181 gaggtctttc gcgccctaca gcctcgcgtg gcgcgttcca tgcataagca ccggtaaacg
     3241 ccttaccggt gcctgcggca aatgctgacg gagggacaaa acgcccggag gctttaacgg
     3301 ccatatgagc caaaagcctc agaacaacgc ctgctgagtt gctcaacctg cgcgtttaaa
     3361 tcagcaaaag actttgcgct tccggtcaga atatcgtgat gcatcaggcc ggaaacgagc
     3421 tggcttaatt tcgggtaata accaaccacc gccagccact cctgaccggc gtttttaccg
     3481 ctttccgctc tctttttctc gtggagaata aactgaaagc tgtcactggt aacgacataa
     3541 cgttcgccaa tttcaatacg aatactcatg ccgttctccg gtaatgtttg ttttttgctt
     3601 caaagactga ctgacaggaa acacaacgcg tggctgacgg ataagccgca cgacgggcag
     3661 caggtattgg cgcatcacac tcttcgcaaa ccagcgcaga agcacagcaa tgttttaccc
     3721 ttgccgcgtt aatctggcgc tccagtaatt cagcctgttg ttcctgaata aaatctacgt
     3781 tgtccggcat taccagctcc ttttgtcgtt cagcttctta aattcatcag cgcaataact
     3841 ggcgagttct gtcattaatt ttgtcagttc atccactgag gaaacttgct tgtggaatac
     3901 agcgcgttta acaagtaaat tgaccacatc agacaggagg tttaattcac tctgataaat
     3961 tgcgataaca gattcagtta tttcgcgctt ctctttatca ataccaagtt gaataagaga
     4021 caaatcacca tttttcataa cggcgatttt taaggcgtta ttcagtaata caaccgaacg
     4081 agaacaggac atcaaagtac ctccccgcga gacaatccga tattgtgaaa tttttccgac
     4141 tcctgactga gcagctcgac tatctccacg cgggataact ccgcctttgt gatgtggcga
     4201 atcatggcgt caagatgaga agaaaagcgc gtcgcagcgt cggcctgtgc ttcggttctg
     4261 gcctgttgca gcagtaatgc gtatataccg cactgatttt cagaaactgt atgcatgact
     4321 ttctccaggc aaaaagaagc cccgcacaat taagtgcgtt aaaaactctg gttaattact
     4381 taatgcagat attgctctgg ttttaccgac gtcagaattg tcggcgcata ctcaaacagg
     4441 ctgaataatt cacgtaatgc acggaataaa gcatcacgcc agtaacatga ttcttcatta
     4501 attcgccagt atggctggtt aaattctttt tctgtcagtc gtgcgtgcat aaataaagta
     4561 cggcgctgac tgacggttaa aaaactaata tatgcatact cacttgcgcc aacctgacgg
     4621 cgttttgaga atgccccacg cagttcatca attgcacaaa ccagccgttc acgttcgacg
     4681 tcgttcattt cttcaaaacg catcgttgcg tgacgttgtt ttaactgcgc atgaaagcaa
     4741 actgttagcc gttcgcgctc catcatctga ttataataat cacatgtatc ctgccagcga
     4801 gggacggcaa gatgcttacc aattatccgg cgcatagctg ctggctgttt ttcgacgaga
     4861 ttgagcgtca tcactgtcat ttccagaccc tccggctttt cagaaaggtc agagcctttt
     4921 ttaacggact ctgttttttg gtgcggataa tgattccctt acgcccctta ccgtgggtga
     4981 tggtgaagtc aatcgccctg gggctttcgt tacgcaataa ctgagcaata caacgaggct
     5041 cattcatacg attctcctta acgtggttca ccgagaccta accacatcaa ccagccgtca
     5101 cgaatctctt taggacggct ttcataagcc agttttagtc cgttattcca tgccggaagg
     5161 tatacccaat attcacctgc acgacctgaa gctgattgtg gatcggtcat atcaattaca
     5221 ggcagctttc ctttatcgat catccgacga accgctcctg tcgattttcc tattagtttt
     5281 gcgaactcct gataaggaat cgcatcagtc atgagtgtta cttgcttgct catgtcgtcc
     5341 tctagccctc at
//
LOCUS       DAFAVF010000225         5282 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFAVF010000225 DAFAVF010000000
VERSION     DAFAVF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.227"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..810
                     /locus_tag="LGK42_004208"
     CDS             <1..810
                     /locus_tag="LGK42_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HBK2660379.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQILNVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..5085
                     /locus_tag="LGK42_004209"
     CDS             877..5085
                     /locus_tag="LGK42_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2660380.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLAWLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGD
                     VIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            5097..>5282
                     /locus_tag="LGK42_004210"
     CDS             5097..>5282
                     /locus_tag="LGK42_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBK2660381.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDI"
BASE COUNT         1252 a         1388 c         1686 g          956 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat ccttaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgtggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
     1981 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
     2041 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2101 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
     2161 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtac
     2221 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2281 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
     2341 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagtggggac
     2401 gtcatccggt atgcttatga taatccgcac agtgaattac cggccacgac aacagatgcg
     2461 acgggcagca cccggcagat gacctggagc cgctacgggc agttgctggc gttcaccgac
     2521 tgctcgggct accagacccg atatgaatac gaccgcttcg gccagatgac ggcggtccac
     2581 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aacctcggtg
     2641 aaagacgcac agggccgtga aacgcggtat gaatacaacg ccgcaggcga cctgactgcc
     2701 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     2761 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     2821 agcctgacca acgagaacgg cagccacagc gtcttcagtt acgatgcgct ggaccggctg
     2881 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     2941 ctcacacaga gtgaggatga ggggcttgtc accctctggt actacgatga atcggaccgt
     3001 atcactcacc gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tgaccacggc
     3061 tggctgacag acatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3121 gacgataagg gccgcctgac cggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3181 ctgctgtggc agcatgagac aacacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3241 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta tctggcgggc
     3301 atgaagcttg gcgacacgcc gctgctggag tacacgcgcg accgcatgca ccgggagacg
     3361 gtgcgcagct ttggcagcat ggcaggcagt aatgccgcat ataaactgac cagcacatac
     3421 acccccgcag gccagttaca gagccagcac ctgaacagcc tggtgtatga ccgtgactac
     3481 gggtggaatg acaacggcga cctggtgcgc atcagcggcc cgcgacagac gcgggaatac
     3541 ggctacagcg ccacgggcag gctggagagc gtgcgtaccc tcgcaccgga cctggacatc
     3601 cgcatcccgt atgccaccga cccggcggga aaccgcctgc cggacccgga gctgcacccg
     3661 gacagtaccc tcacggcgtg gccggataac cgcatcgcag aggatgcgca ctatgtttac
     3721 cactatgatg aatacggcag actgacggag aagacggacc ttatcccggc gggtgttatc
     3781 cggacggacg acgagcggac ccaccactac cactacgaca gccagcaccg tctggtgttc
     3841 tacacgcgga tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg
     3901 ggacggcgaa tggcgaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg
     3961 ctgtcgcgta aaccggagat gacgtggtac gggtgggacg gcgaccggct gacgacggta
     4021 cagaccgaca ccacgcgtat ccagacggta tatcagccgg ggagcttcgc gccgctcatc
     4081 cgcatcgaaa cagacaacgg cgagcgggag aaagcgcagc gccgcagcct ggcggagaag
     4141 ctgcagcagg aagggagcga ggacgggcac ggcgtggtat ttccggctga actggtgcgg
     4201 ctgctggaca ggctggagga agaaatccgg gcagaccgcg tgagcagtga aagccgggcg
     4261 tggcttgcgc agtgcgggct gacggtggag caactggcca gacaggtgga gccggaatac
     4321 acaccggcgc gaaaagttca tttttaccac tgcgaccacc ggggcctgcc gctggcgctc
     4381 atcagcgaag acggcaatac ggcgtggcgc ggggagtatg atgaatgggg caaccagctt
     4441 aatgaagaga acccgtatta cctgcaccag ccataccgtc tgccggggca gcagcatgat
     4501 gaggaatcag ggctgtacta taaccggaac cggtactatg acccgctaca ggggaggtat
     4561 attacacaag accccattgg gctggcgggg ggatggaacc tgtataatta cccactgaat
     4621 ccgataataa ggatggatcc tttgggtttg tataatttat atcaattatt atatgatgtt
     4681 tggcatgatg attcatatgg aacatcatca attgatatta ctggcagtgg agatctaata
     4741 tcattaggtg gtcatgcagg acttggcgtt gcgtttgcta aaaagaaagg tgaaatgtta
     4801 tctgatattt gtatttatgc tacagcatgc ggacatgcag gaattggtgg tgggataaat
     4861 gcggctatca catattctga gaccaagtct ttacctacat cgggagtcag caattcagta
     4921 ggtgtaacgg ttggcggcgg agttgggggg cattttgcgt atacttatgt agtggatgtt
     4981 gataatccag aatcatcgac agaatctgtt ggtatcggtg caggtgttga cgcttcagtt
     5041 atgactctgg cttgtagaac gtggcaagaa tgctgggtca attaacagga ggaaatatga
     5101 aaaaatatat atttttagtt ttatttttat tgtgtggttg ttctccagtg gacatagagc
     5161 cagctagaca agaagttaat atctttcgtg gtttatatca gacagggaaa tacagtgata
     5221 tatataaaat cacttcaagt gattttcaga ctgccacatc tgaaaaaaaa tttatcgaca
     5281 ta
//
LOCUS       DAFAVF010000226         5258 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFAVF010000226 DAFAVF010000000
VERSION     DAFAVF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.228"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(111..353)
                     /locus_tag="LGK42_004211"
                     /pseudo
     CDS             complement(111..353)
                     /locus_tag="LGK42_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            532..1182
                     /gene="inaA"
                     /locus_tag="LGK42_004212"
     CDS             532..1182
                     /gene="inaA"
                     /locus_tag="LGK42_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBK2660382.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(1236..1490)
                     /gene="yfaE"
                     /locus_tag="LGK42_004213"
     CDS             complement(1236..1490)
                     /gene="yfaE"
                     /locus_tag="LGK42_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBK2660383.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(1490..2620)
                     /gene="nrdB"
                     /locus_tag="LGK42_004214"
     CDS             complement(1490..2620)
                     /gene="nrdB"
                     /locus_tag="LGK42_004214"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBK2660384.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(2709..4994)
                     /gene="nrdA"
                     /locus_tag="LGK42_004215"
     CDS             complement(2709..4994)
                     /gene="nrdA"
                     /locus_tag="LGK42_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBK2660385.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1268 a         1285 c         1395 g         1310 t
ORIGIN      
        1 aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa attccttaat
       61 aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcaca tcaaattttt
      121 ccattcacca cgagttgttc cgtacacgtt tgtagtaaaa acagtggact gccttctacc
      181 ggcttttctt tagccagcaa cgaaatctgt cgatagaaag ttggttcgag aggaacggaa
      241 cacaattcac ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat gcctaatccc
      301 tgtcgaataa aattcatcgc tgtatccgcc tgattaaatt cacattttat ctgctgggcg
      361 cactattagt ggtagtggct ggtggaatct atgcgctgca aatgaagggt aaaagcggtg
      421 tcaagctagt agtggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc
      481 aggtaaccac ctatagtcat tgtcgttaat aaaaataaca ggcgataggg tatggcagtt
      541 tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag
      601 cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag
      661 ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga
      721 ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg
      781 ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg
      841 actgaagata tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg
      901 ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa
      961 atgcatagca ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca
     1021 gaaggtaatg cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc
     1081 gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa
     1141 gcggactggg agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa
     1201 gacgcataag cgttgtatct ggcactacaa ccaattcaca tctcgatttc aatatcgcct
     1261 tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca
     1321 atccagtcaa cctgacctgc aaccagacgg gtgcgacagg agccgcagta accttcgcga
     1381 cactgatact caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca
     1441 tcctggcaca gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagttgga
     1501 agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga
     1561 cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccatg
     1621 ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat
     1681 tggtgatgta ttcaacgtac tggcagagaa tgtctttatt cagaccaatc atcgaaccgt
     1741 cgcggaacag ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat
     1801 agcactcctg cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc
     1861 gcagcagatt cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca
     1921 ggcgaataat tttggcgttg ccttccatca attcgcgttc tgcaaatgcg aaggaacaag
     1981 caaagctgac gtagaaacga atcgcttcca gcgcgttaac gctcatcagg cagagataca
     2041 gttttttctt cagctcgcgc aggctaacgg tcacagtttt accgttaacg gtgtgggtgc
     2101 cttcgcccag aaggtgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga
     2161 tcccttccgc acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaacagacg
     2221 gatcgttaac gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga
     2281 acgcccaggt ttcgacccag gtttccagtt ccggaataga aataagcggc aatagcgcca
     2341 cgttcggact acgaccctga atggagtcca gcagcgtctg atatttcagg ttgctgataa
     2401 agatgtgttt ttcgtgctcc ggcagcgcct ggtaatcgat acggtcgcgg gagacgtcaa
     2461 cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat
     2521 atttttgctg atcgtagcga gccacgttga ccggctgacc aaaaaacatc ggttctttaa
     2581 gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgtggtttta
     2641 ttacccctca ccctaaccct ctcccgcaag gggagagggg acggtttggt gcaaaggctg
     2701 ggggattatc agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggcacc
     2761 agatcgtcct gcgcgtcttc agcgccgtca cgggtgttct gataatacag cgttttgacc
     2821 ccgaatttgt aggcggtgag caggtctttc agcaactgct gcatcggcac ttttcctgac
     2881 gggaaacgtg acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc
     2941 atgataccta ccagttgcag gtagccatcg ttacccggca tttcccacag cagctcatag
     3001 gcgtcgtgca ggtgctcgta gtccggcacc acctggcgca aaataccgtc tttcgacgct
     3061 ttgatgctga cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgcgaa
     3121 gaggtctcgg acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt
     3181 gattcacgca atgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct
     3241 ttcttatagg tatcgatcgg caggatccct ttcgcgtaag tggtttcgtt aaaccacggg
     3301 cacgcgcctt gctctttagc cagctcatta gacgctttca gcaggtaata ctgaatcgct
     3361 tcgaaggttt tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc
     3421 gccagatagt aagcgaagtt aatcacacca atacccagcg tacgacgccc catcgctcca
     3481 cgtttggcgg ccgggatcgg gtaatcctga taatccagca gcgcgtcaag tgcacgaacc
     3541 gccagaattg ccagctcttc cagttcatcc aggctattaa ttgcgcccag gttgaaagca
     3601 gacagcgtac acagcgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc
     3661 ggcagggcta tctccaggca caggttagac tgacgcactg gcgcgatggc cggatcaaac
     3721 gggctatggg tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt
     3781 tcctgcatca tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg
     3841 tctttctcat atttggtata caggcgttca aactcttcct gatcggcgaa gaatgcgtcg
     3901 tacagccccg gtacgtcgga cgggctgaac agggtgatat cttcaccttt cagcaggcgg
     3961 gtatacatca gtttgttgat ctgtaccccg tagtccatat ggcgcacgcg gttgccttcc
     4021 acaccacggt tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag
     4081 aacagcgttg ccgcaccgcc gcgcacgccg ccctgagagc aggatttcac cgctgtctgg
     4141 aaatgtttgt agaacggaat acagccggta tggaacgctt caccaccgcg aatcgggcta
     4201 cccagcgcac gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtatttc
     4261 acaatcgcgc tggaggtagc gttgatggaa tccagactgt caccgcactc gatcagtacg
     4321 caggagctga actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa
     4381 attttaaatg tggaaaccgc gtcgtaaaaa cgcttcacat attgcaggcg cgtttcacgc
     4441 gggtagttcg agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag
     4501 atttcgccgg tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca
     4561 taagagaagg tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct
     4621 tccgtgtagt cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccacg
     4681 tggtcgtaca gcgcaggcgg ctcaaactgg ccgtaggctt ttttacgcag gtggaagatc
     4741 gccaggcgcg cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggca
     4801 gccttgataa tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg
     4861 gagcgcagct cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga
     4921 acgcgatgga ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc
     4981 agattctgat tcatgtatgt cgtacctgtt tttggaattc tttccgcaat agtttcatgc
     5041 agactacctg tagtacggga agctgcttag aacactatat atagggggta tgtttgggat
     5101 tcactgcaag atagtgtgaa aatggccctc ttgcaagtgc ataactttgt ggataactca
     5161 ggaaggaaaa agtggctttc gcgcacctta ggtcagacaa ggtgtccggg aaagtcaatg
     5221 ggaagagaaa aatttgttaa aaataactgt tcggcgat
//
LOCUS       DAFAVF010000227         5059 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFAVF010000227 DAFAVF010000000
VERSION     DAFAVF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.229"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            164..1138
                     /gene="hemB"
                     /locus_tag="LGK42_004216"
     CDS             164..1138
                     /gene="hemB"
                     /locus_tag="LGK42_004216"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBK2660386.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1244..2095)
                     /gene="tauD"
                     /locus_tag="LGK42_004217"
     CDS             complement(1244..2095)
                     /gene="tauD"
                     /locus_tag="LGK42_004217"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBK2660387.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2092..2919)
                     /gene="tauC"
                     /locus_tag="LGK42_004218"
     CDS             complement(2092..2919)
                     /gene="tauC"
                     /locus_tag="LGK42_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBK2660388.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2916..3683)
                     /gene="tauB"
                     /locus_tag="LGK42_004219"
     CDS             complement(2916..3683)
                     /gene="tauB"
                     /locus_tag="LGK42_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBK2660389.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3696..4658)
                     /gene="tauA"
                     /locus_tag="LGK42_004220"
     CDS             complement(3696..4658)
                     /gene="tauA"
                     /locus_tag="LGK42_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK2660390.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1201 a         1437 c         1251 g         1170 t
ORIGIN      
        1 aaaatatttt cagtgaatat gaaattattg ctatttctat tacaaagatg tcctggcctc
       61 tcttccattc tcttcttgtc aacatcgcga caactttcgt aaaacatccc taccctgctt
      121 caggtatact atgcccctcg attccacaaa catcaggcag accatgacag acttaatcca
      181 acgccctcgt cgcctgcgca aatctcctgc gctgcgcgct atgtttgaag agacaacact
      241 tagccttaac gacctggtgt tgccgatctt tgttgaagaa gaaattgacg actacaaagc
      301 cgttgaagcc atgccaggcg tgatgcgcat tccagagaaa catctggcac gcgaaattga
      361 acgcatcgcc aacgccggta tccgttccgt aatgactttt ggcatctctc accataccga
      421 tgaaaccggc agcgacgcct ggcgggaaga tggactggtg gcgcgtatgt cgcgcatctg
      481 caagcagact gtgccagaaa tgatcgtcat gtcagacact tgtttctgtg aatacacttc
      541 tcacggtcac tgcggtgtgc tgtgcgagca tggcgtcgac aacgacgcga ctctggaaaa
      601 tttaggcaag caagccgtgg ttgcagctgc tgcaggcgca gacttcatcg ccccttccgc
      661 cgcgatggac ggccaggtac aggcgattcg tcaggcgctg gacgctgcag gctttaaaga
      721 tacggcgatt atgtcgtatt cgaccaagtt cgcctcttcc ttttatggcc cgttccgtga
      781 agctgccgga agcgcattaa aaggcgaccg caaaagctat cagatgaacc caatgaaccg
      841 tcgcgaggcg attcgtgagt cactgctgga tgaagcccag ggcgcagact gcctgatggt
      901 taaacctgct ggagcatacc tcgacatcgt gcgtgagctg cgtgaacgta ctgaattgcc
      961 gattggcgcg tatcaggtga gcggtgagta cgcgatgatt aagttcgccg cgctggcggg
     1021 tgctatagat gaagagaaag tcgtgctcga aagcttaggt tcaattaagc gtgcgggtgc
     1081 ggatctgatt ttcagctact ttgcgctgga tttggctgag aagaagattc tgcgttaatt
     1141 tttttcctct cagaaagggg gagttaacct gttgaaagta cggtgaacgc cccctcaccc
     1201 taaccctctc cccaaggggg cgagggaacc gtccgcactc gtatcacccc gcccgataaa
     1261 acggtttatc cccaaggatc gtcgcccgat gcataatccg ccgctgtggc aggtaatcgg
     1321 cattggcata gtgctgagtc acgcggttat cccaaatcgc aatatcattc ggttgccagc
     1381 gccaacgcac ctgaaactcc ggtttagtga tatgggcgaa taaaaagcct aacaaggctt
     1441 cgctctcttt ctcgctgaca tcgacaattc gcgtagtaaa gccttcattc acaaacagcg
     1501 cctgtttacc gctcaccgga tgcgttcgca ccaccggatg tagcaacgga ggatttttcg
     1561 cgaccgcctc gcgccagcgt tgatgttcct cctcggtttt gcggtatttg tattccggga
     1621 acgatttacg gaaatcatgc tccgcacgca gcccgctcag cagctggcgg aagggaacag
     1681 agagcgcctc ataggccgca ataccgctgg tccagagcgt atcaccgccg gtcgaaggta
     1741 actctttagc tgccagaatc gcccctgcgg gtggcgtttc aataaatgtc acatcggtat
     1801 gccagttgtc gttatccggc ggattatcgt tatgggtatc cagcacgatg atctcgtcaa
     1861 ccccttcggc atgagggtaa acagggtgaa tatgcagttc gccaaaacgc tgggccagcg
     1921 cgcgttgctg ctgcggcgta atagtttgat cgcgcagaaa caccacctga tggcgcagca
     1981 ccgcatggta aagctgttca aactgattat cgcttaacgg gcgcgtcagg tcggcacccg
     2041 aaatttgtgc gccgatatac ggccccagcg gggtaatgct cagacgttca ctcattgtac
     2101 ttctccatgc cagggcgtca gacggcgctg taacgcgcgc aaacctagtt ctaaaagaaa
     2161 ggcgataatc gcaatcaccg cgattcccgc cagcaccacg tcagtcgcga gaaattcacc
     2221 cgctgactga accataaatc ctaaaccgcg cgtcgcggca atcagctccg ccgctaccag
     2281 cgtagaccag cccacaccca gaccaatgcg taatccggtg aggatttccg gcagcgcgcc
     2341 gggcaaaatg acaaaccaca gcacctgcgc acggctggca cccagcgact gggcggcacg
     2401 aatgcgaacc tgctgcgcgc ttttcacccc cgccagcgcc gacatcgcca ccggtgcaaa
     2461 aatcgctaaa tagatcagta agatcttcga ggtttcacca ataccaaacc agatcaccat
     2521 cagcggcaaa taagccagcg gcggcaccgg acgataaagt tcgattaccg gatccagaat
     2581 gccgcgcacc gtcgggctta gccccatggc gatcccgacc ggaataccga tcaccaccgc
     2641 cgcgagtagc gccagcacaa tgcgcgtcag gctggctgcc agatgctgcc acagcgtggc
     2701 gtccataaat ccctgcggtc cggcaatggt gagcagtttt gccagtactt gttgcggcgg
     2761 cggcaaaaac agcgggctaa tcagttgcag cgccgccacc gtccaccata cagtgagtaa
     2821 aaccgctaac gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc gccatttcag
     2881 ccgccgcgaa tgcagttttt cattaatgag cacactcatg agaacgcctc ccgttgctca
     2941 aatacgcggc ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg
     3001 cggctcgact ctcccgcaac aaagcggcga gcaaagttga gcggcagccg ctccagcaca
     3061 cggccagggc cggatgaaag cagaaccagt tcagtcgcca taaacaccgc ttcttctata
     3121 tcgtgggtaa tcaacagcac ctgcttgccc gtctcctgcc agagtttcag cagcagagtt
     3181 tgcatctggt cgcgggtgaa ggcgtccagc gcaccaaacg gttcgtcgag taataacagc
     3241 tggggattcg ccgccagcgc gcgggcaatc cccacccgct gacgttgacc accggaaagc
     3301 tgccagatat agcgtttttc tgcgccttcc agccccactt ttttcagcat ctggtgcgcg
     3361 atttccagtc gctgcatttt ctctatacct gccaattgca ggccgaacgc cacgttgtcc
     3421 tgtacattgc gccacggtag tagcccttca ttctgaaaaa ccaccccgcg ctctgctccc
     3481 ggtccctcaa tacgcttacc cgccagttga atgctgccat gctgataagg cacaaaaccg
     3541 gcaatcagat tcagcagggt ggttttaccg cagccggacg gccccagcac caccagtagc
     3601 tcgccgcttt ccagcgtcag gttgatatct tccagtaccg gtttgccgcc ataatcggcg
     3661 taaagatgag agatttgcag catctgcgcc tccttttatt gcacgaagcg cgaggtaacg
     3721 tactggctgt aatcattcgc tacagccggg accttgccct gctctttcaa aaactgcgcg
     3781 gtgtcgatga tcgctttgtt caccggtccg gtcagttcta ccgtttgttg ctgcggcgtc
     3841 agataggtat tccccttcac cagccccgga acgtcacctt ccggcacgcc gcttaaacgc
     3901 gccagtttgc tgatgttttc cggctgtttc agccacgcgt ctgggttagc aatgtacggt
     3961 tgctgagcat cgatggcgct tttagcgaac gctttcacga cctcaggatg tttctcggca
     4021 aaatctttgc gcaccaccca gacgtccagc gttggcgcac cccactgccc gacctgttca
     4081 gaatcggtca acaccttgcc gtctttttcc agggcgttaa ccgccggtgc ccagacataa
     4141 gcaccatcaa tatcaccccg ctgccatgcg gcgataatcg cgggcggctg caggttcaca
     4201 atctccactt gccctggttt aatcccccag tgtttcagcg ccgccagcag gctgtagtgg
     4261 gtggtggaga taaacggtac ggcaatgcgt ttgccaatca aatcttcggg tttgctgata
     4321 gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa gaagacttca
     4381 atcggcacct gttggctggc tgcaaccgct aatgggctgg aaccgaggtt gccgatttgc
     4441 acgtcgccag aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc
     4501 acggttgctc cgctttcttt agcaaaggtg ttgtcggcct gagccacttt cgccggttcg
     4561 gctgaggttt gatacgccac ggtgacgttc accgcctgtg cctgaaaagc gatgaatgcc
     4621 agtgcggcaa gaagtgtgtt acgcgatgaa attgccatga ttgtctgctc ccctgtcttg
     4681 ttatgggagc agtattcagg aataaaaaca ttcattaaaa gaattagtcg ttatcgcaca
     4741 gatgatttta ttcttagcaa aaaaacggct aaggaaagat taaatatgca attactccgt
     4801 tctaacaatt gggaaaatta ctacacttca ttttagtgat gcataaacaa ccacattacg
     4861 agatttatta cttcattatt tctatcaata tcaatacata taataaaact attatattcg
     4921 cattgtatat atcactgtat cattctcaat cactcgcata ttacattcac ataatgtata
     4981 taaacaatac ttgttcacag aacatatttt ttaaatgcaa ttaattgaca aactaaatca
     5041 catctaaata caatgtata
//
LOCUS       DAFAVF010000228         5008 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFAVF010000228 DAFAVF010000000
VERSION     DAFAVF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.230"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(168..500)
                     /locus_tag="LGK42_004221"
     CDS             complement(168..500)
                     /locus_tag="LGK42_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FlxA"
                     /protein_id="HBK2660391.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(703..1008)
                     /locus_tag="LGK42_004222"
     CDS             complement(703..1008)
                     /locus_tag="LGK42_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660392.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            1033..1272
                     /gene="relB"
                     /locus_tag="LGK42_004223"
     CDS             1033..1272
                     /gene="relB"
                     /locus_tag="LGK42_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBK2660393.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            1272..1559
                     /gene="relE"
                     /locus_tag="LGK42_004224"
     CDS             1272..1559
                     /gene="relE"
                     /locus_tag="LGK42_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBK2660394.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            1631..1786
                     /gene="hokD"
                     /locus_tag="LGK42_004225"
     CDS             1631..1786
                     /gene="hokD"
                     /locus_tag="LGK42_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBK2660395.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2003..2254
                     /locus_tag="LGK42_004226"
     CDS             2003..2254
                     /locus_tag="LGK42_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660396.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            2321..2599
                     /locus_tag="LGK42_004227"
     CDS             2321..2599
                     /locus_tag="LGK42_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660397.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            2601..3650
                     /locus_tag="LGK42_004228"
     CDS             2601..3650
                     /locus_tag="LGK42_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBK2660398.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWTVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            3664..4416
                     /locus_tag="LGK42_004229"
     CDS             3664..4416
                     /locus_tag="LGK42_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBK2660399.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(4694..4783)
                     /locus_tag="LGK42_004230"
     CDS             complement(4694..4783)
                     /locus_tag="LGK42_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660400.1"
                     /translation="MNNPVCLDDWLIGFKILCCTLAVIALLII"
     gene            complement(4838..>5008)
                     /gene="cspF"
                     /locus_tag="LGK42_004231"
     CDS             complement(4838..>5008)
                     /gene="cspF"
                     /locus_tag="LGK42_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HBK2660401.1"
                     /translation="KSGKGLITPSDGRIDVQLHVSALNLRDAEEITTGLRVEFYRING
                     LRGPSAANVYLS"
BASE COUNT         1317 a         1085 c         1277 g         1329 t
ORIGIN      
        1 tgggttgtca gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact
       61 tatatcttgt aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag
      121 gctgatggcg aaaagtggcc cgatgagggc cacaatacgg ctgtcactta gacgtaaata
      181 tcaatggtgc cagcggtatt tgtatcgtct tttttctctt cttttttatc aggctgaact
      241 gtcgcgtctt cattcttttt ctctgcctgc tgccttaaca actgctccag ttgagcccag
      301 aggctttcaa tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc
      361 ccctcttccg atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt
      421 ttggctattt ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt
      481 ccctgaattg tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgtacaaa
      541 aaaatcttta atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctcg
      601 cgggctggcg cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt
      661 ttctgccgca tgcgggtgtt gcataaaacg tgttacgttc ctttatcgac aggtcaggtc
      721 accgctcacc cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata
      781 agttgagttg tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag
      841 atgatggccg gactctttaa aaacgagctg acctgcacaa tacaggatgg acttagcaat
      901 ggctgctcct ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc
      961 ttcgtgactt gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa
     1021 gaggtgtaag acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct
     1081 tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag
     1141 tatatcgctg acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct
     1201 gaacttgtgg agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg
     1261 gatgaactct gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa
     1321 agctgggctc gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac
     1381 cccggattga agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt
     1441 cttcaggcta tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt
     1501 ctgttgggaa aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa
     1561 ccaaagcatg acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga
     1621 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     1681 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg
     1741 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg
     1801 gagaaatccc tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg
     1861 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttctggacgg tgccggaata
     1921 gaatcaaaaa tacttaagta gcgcgcaggg ataagaggga tggtccctta aaggggagag
     1981 ctaattatcc ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
     2041 agatggcctc tatgctgtgt gcgttgaaaa tggaaatctt gttagtcatt accgcattat
     2101 gtgtttgcga aagaatgggg ctgcgttaat taattttgtg gatgctcggg tcacggacgg
     2161 atttatcttg cgcgaaggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
     2221 aaaagctggc ttttctgctt tttcaggaga ataaactcat ctacaatctt gcgcggggct
     2281 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
     2341 ttacaattct gataattcgc ccgttcttgc ctgcacgcac gggcggtatt ctcacgcatt
     2401 caagtctgaa tggttccagc accctccatg cactgcagaa caggccgaat ggctgattca
     2461 ttcttaccgc aggcgcgggt tcgaggttaa gaaagctctc agtctcgact atcggcactg
     2521 gataatctct gtcaggctgc cttattccga acgcccacca cgtgcgtccc gcactttcca
     2581 gcaacggatc tggaggtaac gtgcgggtat tacttagacc tgttctggtg cctgagcttg
     2641 ggctggtggt ccttaagccg ggccgtgaat ccatacagat atttcataat cctcgagtgc
     2701 tggtggaacc ggaaccaaaa agcatgcgta atctgccatc cggagtcgtt cctgccgttc
     2761 gccagccgct ggcggaagac aaaacattgc tgccgttttt tagtaacgaa cgggtgattc
     2821 gtgctgctgg cggcgttggc gcattgtccg actggctatt acgtcatgtt acatcctgcc
     2881 agtggcctaa tggcgattac catcacactg aaacagtcat tcaccgttat ggtaccggcg
     2941 caatggtgtt gtgctggcac tgcgacaacc aactgcgtga ccagacatcg gaatcactgg
     3001 agctgcttgc tcaacaaaat ctgacagcat gggtgattga cgtcatccgt cacgcaataa
     3061 gcggtacgca ggagcgggaa ttatctttgg ctgaattatc ctggtggacg gtctgcaatc
     3121 aggtggtgga tgcactacct gaggctgtat cgcgtcgttc gctgggatta ccagcggaaa
     3181 aaatctgctc ggtgtaccgc gaaagcgaca tcgtaccggg agagcagacc gccaccagca
     3241 tattgaaaca acgcacaaaa aatcttgcac cgttgcctta cgcccaccag caacaaaaat
     3301 caccacagga aaagacggtg gtaagcatca ccgttgatcc agagtctccg gaatctttca
     3361 tgaagctgcc taaacgtcgc cgctgggtta aggagaaata cacacgttgg gttaagacac
     3421 agccgtgtgc ttgctgcggt atgccagccg acgatccgca tcatctgatt ggtcacgggc
     3481 agggcggaat gggaacaaaa gcacatgatc tctttgtgtt gcctttgtgc agaaagcatc
     3541 acaacgagct gcatacggat acagtggcat ttgaagataa gtatggctcc caactggagc
     3601 tgatatttcg ttttatcgat cgcgcgctgg caattggcgt actggcgtaa gtggagaacg
     3661 agcatgaacc ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagcgat
     3721 gacgctccag cgacaggcac cggttgttta acaattacgg atgtaatggc agcgcagggg
     3781 atggtgcagt cgaaagcacc acttgggttg gccttatttc tggcaaaagt tggtgttcag
     3841 gaccctcagt ttgcgattga aggcctgcta aattacgcga tggcactgga taacccgaca
     3901 ttgaacaaat tgagtgaaga aatccggtta cagattattc cttacctcgt gagttttgcc
     3961 tttgctgatt actccaggtc tgcggcaagt aaggctcgct gtgagcattg ttcaggtacg
     4021 ggattttata atgtattgcg cgaagtggtg aaacactaca gacgcgggga atctgtaatc
     4081 aaggaagaat gggtgaagga actatgtcag cattgccatg gtaagggcga agccagcaca
     4141 gcgtgcagag ggtgtaaggg taaagggatt gttctggatg aaaaaagaac ccggtttcat
     4201 ggcgtaccgg tatataagat ttgtgggcgt tgtaatggaa accggtttag tcgtttaccg
     4261 accacgctgg cacgacgtca tgtccagaag ctggtaccag acctgaccga ttatcagtgg
     4321 tataaggggt atgcggacgt cattggtaaa ctggtaacaa agtgctggca ggaagaagca
     4381 tacgcggaag cgcaattgag gaaggtgacg agataaatga tttttgctga agatggcgac
     4441 atgatgtttg catttttcaa aaaatatgga taaaattttt tcaacgatgg gctttgtata
     4501 cccgacgtta agaaaaagta gaaaacccgc tgatgagcgg gttttgtgct ttaaatgggg
     4561 caatggtaat gttgaatctc atcccgggac tcatgtctgt taacttatta tttagctggt
     4621 gacttggtta tttgcctgat gtttaaaatg ttttcttcca gtacaatgtc cctaaacaca
     4681 atgagtctgc ttattatatt attagcagag ctattacggc caaagtacag cataagattt
     4741 taaagccaat caaccagtca tcaagacaga cggggttatt cataaaaact ctccatgtgt
     4801 gatccgatgg ggcctgaaat taaagcttta atatagctca tgaaaggtaa acattggcag
     4861 ctgaagggcc acgcagacca tttatccggt aaaattccac gcgtaatccg gtggtaattt
     4921 cttctgcatc gcggagattg agcgctgaaa catgaagctg gacatcgata cgaccatcgg
     4981 atggggtgat aagacccttg ccgctttt
//
LOCUS       DAFAVF010000229         4974 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFAVF010000229 DAFAVF010000000
VERSION     DAFAVF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.231"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            224..676
                     /locus_tag="LGK42_004232"
     CDS             224..676
                     /locus_tag="LGK42_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2660402.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
     gene            complement(709..894)
                     /locus_tag="LGK42_004233"
     CDS             complement(709..894)
                     /locus_tag="LGK42_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660403.1"
                     /translation="MMDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEI
                     DPRQSISVHLPYSQTYL"
     gene            1298..1540
                     /locus_tag="LGK42_004234"
     CDS             1298..1540
                     /locus_tag="LGK42_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBK2660404.1"
                     /translation="MSGEPAEFARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1572..2222)
                     /gene="tetR(A)"
                     /locus_tag="LGK42_004235"
     CDS             complement(1572..2222)
                     /gene="tetR(A)"
                     /locus_tag="LGK42_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBK2660405.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            2328..3527
                     /gene="tet(A)"
                     /locus_tag="LGK42_004236"
     CDS             2328..3527
                     /gene="tet(A)"
                     /locus_tag="LGK42_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBK2660406.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3559..4443)
                     /locus_tag="LGK42_004237"
     CDS             complement(3559..4443)
                     /locus_tag="LGK42_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBK2660407.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4581..4973)
                     /locus_tag="LGK42_004238"
     CDS             complement(4581..4973)
                     /locus_tag="LGK42_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK2660408.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT         1040 a         1427 c         1485 g         1022 t
ORIGIN      
        1 atgaaaatca tatctgatat gattttatgt gaattgctta attatcatat cgtttgcccg
       61 tttcccgccg tgcagatccg gtttccggct gaagtggatc cagacctgat ttgttgctga
      121 gtatcggtaa aagtgcggct tataagctat tctgaaacat ggcaactgaa tctgggctgt
      181 ataattccgg caagtgtcat ctgtatttaa aggacgtgtg agtatgaaat ttatggaatg
      241 tgctgtccgg gatgtgattt atgggaccaa tgtccgtatt gtcaaaccgg tcaacattta
      301 tgaatgtgaa ctcagggaca atgtgtttgt cggccccttc gttgagatac agaaaggctg
      361 tgtcattggt agtggaagtc ggatacagtc gcacacgttt atttgtgaga acgtaacact
      421 gggggagaac tgcttcattg gtcataacgt gacgttcgcc aacgatctgt ttcgttcagg
      481 agcaccagat ccgtcaccgg ataactggat cagcattatc ctgggggatt cggttactgt
      541 tggcagtggc gccattattc tgtctccgta tatatgcagc ggagctgtta tcggtgcggg
      601 ggcagttgta gtaaaaccgg ttgagaacaa aggcatttat gcaggaaacc cggcaagact
      661 gatcagaata ttatgaaaaa agaaaagggc cgttactggc cctttttatc agaggtaggt
      721 ctgtgaatac ggcaggtgta cagaaatcga ctgccgggga tcgatttcca gggccgcccg
      781 gtattctgcg tcagtgatat cacctttttt cagtaaataa ccgagagcat cgtttcccag
      841 ccctgacagt atacgtattc tggcgatccg gaaatcaaac aggctgtcca tcataccatg
      901 ctccggaaat gcgggaggag tggccagata ggcaggtttg accatactgc tgatgatgtt
      961 ccagtatcgg gaaaagtcgc gggccgggtc cgggagaaga cctgacagca gcccaatccg
     1021 gatggtttcc agccagctgt cataaaatcg cttaacatga agtgaagcca tgtgctcagc
     1081 acaccatttg cgcatagctt ctttttccgg aagggaagtg ttcccggaga atacgttcat
     1141 tacccactgg ctgatgatat caaaataggg aaacgcagcc ccgaaaccgg cggtatccac
     1201 cgggggatta atgacggcca gagaagggtc ctctgtccag aaaaagtgct catagagctg
     1261 tgttctgtca aatccctgaa tgaacgagta atcaggcatt tcaggggagc ccgcagaatt
     1321 cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg
     1381 gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
     1441 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
     1501 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
     1561 agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc
     1621 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
     1681 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
     1741 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
     1801 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
     1861 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
     1921 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
     1981 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
     2041 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
     2101 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
     2161 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
     2221 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
     2281 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
     2341 gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
     2401 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
     2461 atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg
     2521 cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg
     2581 tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc gggcggatcg
     2641 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
     2701 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
     2761 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
     2821 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
     2881 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
     2941 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
     3001 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
     3061 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
     3121 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga
     3181 ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc
     3241 cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt
     3301 ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca
     3361 gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa
     3421 cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc
     3481 tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat
     3541 aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc taggagtgcg
     3601 gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga tgatttgaag
     3661 cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc ccgggctgag
     3721 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
     3781 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
     3841 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
     3901 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
     3961 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
     4021 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
     4081 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
     4141 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
     4201 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
     4261 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
     4321 ccgcagcatc gcgaccgtca tcggtgagaa gttcggcagg tattgggtgg tgacaatgta
     4381 ggtgctgccc caaatggcag gtgctatcgc tgtgaacaat aaatcgggcg tgcgtaatga
     4441 catgcgatta caagacctcc gctacggcga tgggtttttg agagttgcag gcgccgcgat
     4501 gaccgcaccc tgacttggac tggccctgcc tcgcaccaat ccacctgcgg ctatggccgc
     4561 gatcatgcga ggcccgaaaa ttacacgacc tgtacacgtc cgccgaaccc ggaacgcaac
     4621 agagcttcgt gggcatcccg atccgggtcg tagcagcagt cctggaccaa acgcacgtcg
     4681 tagtccgcat cactagccca ggcgacgctt gaaagaacaa cgccggtggt gcttatcccg
     4741 gccatgacaa gcgtgcttac gccgcgcgtc cgaaggtcgg cgtcgagcgc cgtgccataa
     4801 aagacgctgg ctcgcggaca tgcatagaaa aggtcgcccc gttcgatcgc aagcccttcg
     4861 acgggcagac cggtgcgaaa tcgtccgctc gggagatacg gtgagatttg gcgattcgtc
     4921 gccggcggcg catgttcata ctcttcgccg agcgaaaagt tggggaacag cacg
//
LOCUS       DAFAVF010000230         4900 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFAVF010000230 DAFAVF010000000
VERSION     DAFAVF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.232"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            1..2393
                     /gene="htrE"
                     /locus_tag="LGK42_004239"
                     /pseudo
     CDS             1..2393
                     /gene="htrE"
                     /locus_tag="LGK42_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
     gene            2410..2979
                     /locus_tag="LGK42_004240"
     CDS             2410..2979
                     /locus_tag="LGK42_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2660409.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            2994..3596
                     /gene="yadL"
                     /locus_tag="LGK42_004241"
     CDS             2994..3596
                     /gene="yadL"
                     /locus_tag="LGK42_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBK2660410.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            3623..4219
                     /locus_tag="LGK42_004242"
     CDS             3623..4219
                     /locus_tag="LGK42_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2660411.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            4272..>4900
                     /locus_tag="LGK42_004243"
     CDS             4272..>4900
                     /locus_tag="LGK42_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBK2660412.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDT"
BASE COUNT         1464 a          987 c         1086 g         1363 t
ORIGIN      
        1 tatgtcaacg atcaacctat tattaaccaa agcattacat ttattgaaat tgaaggaaaa
       61 aaaacgcaca agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg
      121 atataaataa cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta
      181 atttgacgga aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg
      241 atatagacgt tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt
      301 tatgggaaaa tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg
      361 aaactcctgg tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag
      421 gtgcatggcg actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt
      481 atgattttaa gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc
      541 ttggtgagtc ttatacaacg ggcgaaacct ttgattctgt cagtatacgc ggcattcgtt
      601 tatacagcga cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg
      661 gcgttgctaa taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa
      721 cgactgtgcc gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg
      781 atcttattgt tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt
      841 catcggtcgt tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg
      901 tcttaaaaga tgatattcag gatgagccta atttatttca ggccagctac tactacggtc
      961 tgaataacta tctaacgggt tatactggta ttcagattac cgataataac tataccgctg
     1021 gtttgttagg tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact
     1081 ccaatgttcg tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga
     1141 acaagttatt cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac
     1201 agaattacct tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac
     1261 aagatcttga accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca
     1321 gtattaacca accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa
     1381 gttggtccga ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta
     1441 acagtgcatc ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg
     1501 acactgacga tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg
     1561 aacaacggac ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta
     1621 ataaccaact caacgtcagt agcagtggct atagtgataa cgctcgcctc agttatagcg
     1681 taaatactgg ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg
     1741 ccagctatga atcaccgtgg ggaaccctgg caggttcagt ttctgcaaat agcgataaca
     1801 gtcgtcaagt gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct
     1861 tcagtaatga cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc
     1921 aaggagcgcg aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca
     1981 gcgctctttc tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg
     2041 atgttgaatt aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg
     2101 ctgattttga aaccgtgcaa gggcaatcag ccattatgaa catcacacga agtgatggta
     2161 aaaatattcc atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg
     2221 gacagggtgg acaagcattt gttcgtggta ttgagcagca gggaaatatc agcattaaat
     2281 ggctcgaaga aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag
     2341 aaaaaatagc gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag
     2401 ggacgtcaaa tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta
     2461 tcaccatcag tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca
     2521 acctgtaaca tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca
     2581 ttgagaatcc ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct
     2641 gattttaaac tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg
     2701 accggaaatg catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct
     2761 tcgacgacaa gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc
     2821 cttaaaccta acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag
     2881 ggtcttgaaa tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat
     2941 tttcgtgcac tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat
     3001 ttaaaaattt acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg
     3061 taccctctta tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag
     3121 gtacttgtac tgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg
     3181 gcgatgtata tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat
     3241 tcaaagactg tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca
     3301 catgcgaagg tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccgcagata
     3361 aagcaagtgc cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa
     3421 cacaatttag ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg
     3481 cggttgttta ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca
     3541 atgtcactgc gggtaaattt tctgcacctg ccacatttac agtaacctat aactaacaga
     3601 ctgacagatc caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat
     3661 tctgtttctg tcactacagt ttcccattac ctatccagcc atcgctgggc aggatattga
     3721 ccttgttgcc aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat
     3781 tgatcttggc gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca
     3841 accaggtgga aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga
     3901 tgtgcttaat cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc
     3961 aaggcaaata tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt
     4021 ttccattcag gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc
     4081 cgtgtatcca gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat
     4141 gcaaaaaatc gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt
     4201 tgatgtgtac tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg
     4261 gtggagtgat aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt
     4321 gttataccgc gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg
     4381 gcgttggtcc gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtca
     4441 tattcagata tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac
     4501 ctacaggtgt aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt
     4561 attgcaacag aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg
     4621 actcaggtaa aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt
     4681 acacattatt aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac
     4741 ctgggatata cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag
     4801 acgtgttata ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg
     4861 gtatttatca gacccttaca attgaattct atacagatac
//
LOCUS       DAFAVF010000231         4875 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFAVF010000231 DAFAVF010000000
VERSION     DAFAVF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.233"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            90..842
                     /locus_tag="LGK42_004244"
     CDS             90..842
                     /locus_tag="LGK42_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK2660413.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     LEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            856..1932
                     /locus_tag="LGK42_004245"
     CDS             856..1932
                     /locus_tag="LGK42_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK2660414.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKTQWGG
                     FLNAFVSPPADTFAGSLNGVAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACRLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDVEAIASGLTLLAVQAQEK"
     gene            1932..2930
                     /locus_tag="LGK42_004246"
     CDS             1932..2930
                     /locus_tag="LGK42_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK2660415.1"
                     /translation="MNMPARAPVMPLAQTREEINATAAALRHLAAIWGECFSSVQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCSALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSLTVDALSHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYTLLRLAQNLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATAFPLEEFYHSLRLHQAGVGQHYFLLPATSREYPFYIATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2935..3849
                     /locus_tag="LGK42_004247"
     CDS             2935..3849
                     /locus_tag="LGK42_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBK2660416.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYYYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYAAEPDFTLLHDIQDEYQLEAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPIIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLATPSEVNLFLSQHD"
     gene            3879..>4875
                     /locus_tag="LGK42_004248"
     CDS             3879..>4875
                     /locus_tag="LGK42_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBK2660417.1"
                     /translation="MKIRHAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKDIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIE"
BASE COUNT         1425 a         1034 c         1119 g         1297 t
ORIGIN      
        1 tttatgtcac agataacaaa taagcatact gcgcaataaa aataaaagaa tgtaaagttg
       61 gtatcaacca cttgaaatga ggctttgtca tgataagaat ttataaagcc agtaaaggtc
      121 agctaaagca tcgtgaaatt tttgatgaaa taaagaaaaa aattgataac ggtgaatgga
      181 aagaaggcaa agctatacca acagaaagag aactggctga acatttttta agcagcagaa
      241 caacaattcg gaaagcaata gaaagtttga aacaacgcgg atacctgcat agtgtccacg
      301 ggcaaggaac atttacatta ccggctcgca gtcgggaaaa tcatttatta catagtttta
      361 ctgatgacat taaggctcgt ggtggcgtac cggcacagaa tattcttgaa ataggttata
      421 ttcctctcag tgatattatc agaaaaaatc tcgaacttga tatccatgtc cataccgttt
      481 tctgtataaa acgcattcgc tatatgggaa gtacgccgct gggtattcag acatcatggc
      541 ttgcgttaaa tgacggacaa gaaataactc tggaagagct gctggcgacg ggttcgctct
      601 atattttact ggaagaaaaa ctcgctatta aacctttaga agcaacggag attatctctg
      661 cccggcttcc ctcgcccctt gagcgtcaat tactggaact gactgccgat gacgtcgtat
      721 taagttgtac ccgcgtcacg ttatccgtgg aacgtaaacc aatggagtat gtagagatgg
      781 tttatcccgc aagtcggtac tcctacaaag cacgaattac aaaagacagt tttagcgtgt
      841 aacagagaga atattgtgaa caaaatagaa caagccgtaa tttataacaa aatattaggt
      901 tctttagcct gcgcaggtat gggcgatgca ttaggcgcag cgactgaact ctacagcatt
      961 gatgaaataa aaactcaatg gggaggtttt ttaaatgcct ttgtttctcc tcctgccgat
     1021 acctttgctg gctcattaaa tggtgttgca ggacttatca ccgacgattc cagccaaatg
     1081 tatgtgtttt cagaagggtt aattgaggct ggatttgata acttcaccaa taatgactgg
     1141 ttagcttgtc ttctgcgttg ggccgatatg cagccctacg caaattataa aggccccaca
     1201 acagaacaaa tcgtcaaggc actaaaagaa ggtagaccaa caaacaccat tgggcgtatt
     1261 ggaacatctt ctcgtcaggc ccccaatgtc ggcaccacga atggcgcagg aatgcgtgta
     1321 gccccggcag gattgatctg gcctggcaag aaagaaaaag cgtgtcgcct ggcgttaata
     1381 acttgcctac cttcacatga caccaatatt gcgattgcca gcgcttgcgc catcgctgca
     1441 gcgacatcgc aggctatgtt accagaagcc tcgctcacat cattactgga tgccgcaatc
     1501 tggggagcga attacggcga acgcctggcc aaacaatatg cacgatgtgt agcagggcca
     1561 tctatcgcca tgcgcatcca acttgcagcg gacatcgcca gacgtgccaa tgatctggag
     1621 tcttgtctgc gagaaatgga aggtttggtt ggtaactctg ttgccgccca tgaatccatt
     1681 cctgcggcaa tcggtctatt gctgtactgc aaaggagaac cctgggaaac catccatgcc
     1741 tgcgccaata ttggcaatga caccgatagc atcgctacga tggcaggcgc aattgcaggg
     1801 gcatggcgcg gttttgatgc gctccctgaa gataaatatg cctttttccg cgccgtaaat
     1861 aataaagatt tcgatgttga agcgatcgca tcgggtctga cattgcttgc agtacaggca
     1921 caggagaaat aatgaacatg ccagcccgtg ctcctgttat gccattagcg caaactcgtg
     1981 aggaaataaa tgcaacggcg gcagcgctac gccatttggc agctatctgg ggtgaatgtt
     2041 tttcatctgt tcagctcgat aaacgtgctg ttgaacgcgt ctgtatagtg ggatcgggcg
     2101 actcctggac ggtggcacta tgtgccgccg cctggctggg gaaatacaca catctgtttt
     2161 gttccgcatt gcaaacgtgg gattttcttc agactgattt aacccgttat cagaaagaaa
     2221 cgttgatcat tattctcagc gcaagtggca gaccatcact gaccgttgac gcccttagcc
     2281 acgcagtatg ctcaaacgcg caagtgctgg gtgtgacgaa ttgccctgga acaccatttt
     2341 gcgcaataac agaaaacatg ctctatacat gggcgaataa gcagggtatt ccaacgcaaa
     2401 gtagtagcgt cacactttat accctcctgc gcttagcgca aaacttatgt ccaggcttaa
     2461 caccactgca aattgaagag gatttagaag ggaaatttag ccagattaat cagcactggc
     2521 agcaaaagaa gcgccatttt taccaacaaa aagagataac gtttctgggg agtggcttaa
     2581 gttggggact agcaatatcg gggagcaatt tactttcttg cggcccacag attcgtgcga
     2641 cagcctttcc actggaagaa ttttatcatt cattacgctt acatcaggct ggtgtcggtc
     2701 agcattattt tttactgcca gctacatccc gcgagtaccc attttacatt gcaacccaaa
     2761 aagcaattgt tgaacaggga gcgacagcag aattaatcag ttttatacct gatgccagtg
     2821 agtccaacaa tttatttctg gtcatgcagt ggttatatga aatgtgctgg catttaagtt
     2881 gtgattatgt cgacgcaggc gggcaacgcg ttagccatag ggagaaataa gactatgaag
     2941 aaatttgatg tcgccgcttt aggcagtgga aatatagata tttttttatc tatcccgtca
     3001 ctccccactc gtggcggtaa agtggtcggg acacgtctgg gtgaacaagc tggcggcaca
     3061 gtcgccaaca gtgcctgtgc gatgggacaa ctgggtctca atgtggtatc tgtttcatgt
     3121 gtgggcaatg atcattcagc ctcgatcatt cttgatggat ttaaaaaata tcatgtaaat
     3181 tgtgatttcg ttcaggtaat tccggaattg attgccaata ccgcgattat ctttattgat
     3241 gaattaggtg aaaaaacgtt ggtttactcg cctggtagtg atcatgagtg ggataaagaa
     3301 aaagccctgc aggcaattgc gcaaagccgt tattattata cgatgccagc agatatcgag
     3361 aaatttcgta tgctggccga atattcccgt agccagatga caaaagttgt cgttgatatt
     3421 gagcctcata tcattgcaac acctgaacac cttgccagaa ttttacacct tgctgatatt
     3481 gcgatattta attatgacgg ttttattcgt ggctatgcag cagagccaga ttttacactt
     3541 ctgcatgata tacaagacga gtatcaactt gaggctgtcg tggttacgct ggatgttagg
     3601 ggagtaattg cagtcaaagg caatgagcaa gtaaaaatag gtagctatac aattcccatt
     3661 attgatacca ctggcgctgg agatactttt aatggtgcat ttgtttatag cttaataaaa
     3721 aacatgccct taattgaagc actaaaattt gcttctgcca ctgcagcaat aaatattact
     3781 gcattaggtg ccagaggtca tctggcgaca ccatcggaag ttaatctttt tctttcacaa
     3841 cacgactaaa atcagataca tacacaaagg taaatatcat gaaaataaga catgccgcta
     3901 tttttctggc attaccgctg ttatgcacaa cagcgcaggc aaacagtttt cttcattatg
     3961 ctggtgagtt caacttccgg acgggagaaa aacagaacaa tgttacaatc gcggggctat
     4021 cagccatcac atttgatgca aaaaaaaata ttttctatgc tattaacgat agcagaaaca
     4081 ataataaaga aggagatgcc agtctttata cactaaaaat acaggtatca tccaggggca
     4141 ttgagcaagt gaattttctc aaccagcgtc cactgcttga tgcaaaacag cagcctttcg
     4201 tgaccaatac ggtggatgcc gaaggtctgg ccctgacaca caatggtaag tcattactct
     4261 ggagttcgga gttaggtgct ccactgcgtc tgagcactct cgatggcgta atggagaaag
     4321 attttacgtc tttatttcct gctcgattta atattagttc tgacaaagag agcagcaatg
     4381 gtattcgcag cggcaacgcc tgggagggcc tgacagttac accagatgga aaatcacttt
     4441 ttatcgccgt tgaaagcagc cttaaacagg atgggcctat tgcaagtcca ataaattcag
     4501 gtacatcacg cttactgcaa ttttctatag atgcagacgg tcgaccttca aagcaattgc
     4561 atgaatatct ctatattact gatcctgttc cgcaagtgag taagttcggt attaatgata
     4621 atggcgtatc cgaggtcctg gcgctgaatg atcaccaatt gttggtcatc gaacgttccg
     4681 gcagaaacgt cagtgctggg ttcaatgact gggattactc tgtcagagtt tatatggtcg
     4741 atttaactgc tgccagcgat atcaaggata ttgacagtct gcaagattgg tccaataaat
     4801 ccacgttaca acccgtcagt aaaaaattat taattgattt tgctgattac acttcctctg
     4861 cggattgtat tgaag
//
LOCUS       DAFAVF010000232         4827 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFAVF010000232 DAFAVF010000000
VERSION     DAFAVF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.234"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            21..845
                     /gene="rcnA"
                     /locus_tag="LGK42_004249"
     CDS             21..845
                     /gene="rcnA"
                     /locus_tag="LGK42_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBK2660418.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1064..1402
                     /gene="rcnB"
                     /locus_tag="LGK42_004250"
     CDS             1064..1402
                     /gene="rcnB"
                     /locus_tag="LGK42_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBK2660419.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1484..2518)
                     /locus_tag="LGK42_004251"
     CDS             complement(1484..2518)
                     /locus_tag="LGK42_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBK2660420.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRRSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(2532..>4827)
                     /locus_tag="LGK42_004252"
     CDS             complement(2532..>4827)
                     /locus_tag="LGK42_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2660421.1"
                     /translation="NKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGKQCLT
                     FEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYVSQYY
                     SDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLL
                     GTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQN
                     GFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVS
                     KYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRF
                     GAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDH
                     VWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYW
                     GRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDVTTPR
                     RQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANLTWNA
                     PVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNG
                     QKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFD
                     TDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVNVAID
                     KQQGLSCTITFGKEIDESRNYICQ"
BASE COUNT         1287 a         1190 c          981 g         1369 t
ORIGIN      
        1 taatactaag aattattatc atgaccgaat ttacaactct tcttcagcaa ggaaacgcct
       61 ggttcttcat ccccagcgcc atcttacttg gtgcgcttca tggcctggaa ccagggcact
      121 caaaaacgat gatggcggcg tttatcatcg ccatcaaagg caccattaaa caggcggtga
      181 tgctcggact ggcagcaact atttcgcata ccgcagtggt ctggttaatt gcctttggcg
      241 ggatggtgat cagcaagcgc tttactgctc aatcagcaga accgtggctc cagctgattt
      301 ccgcagtgat cattattggc accgcgttct ggatgttctg gcgtacctgg cgcggcgaac
      361 gcaactggct ggagaatatg cacgagtatg atcatgaaca tcatcaccac gatcacgaag
      421 atcaccacga ccatggacat catcaccatc acgaacatgg cgagtatcag gatgcccatg
      481 cacgagccca tgccaatgat attaaacgac gctttgatgg tagagaggtc accaactggc
      541 aaattttgtt atttggctta acaggtggtc ttatcccctg tccggcagca attaccgtgc
      601 tgttgatttg cattcagttg aaagccctga cactgggcgc aacactggtc gtcagtttca
      661 gcattggcct ggcgttaacg cttgtcaccg taggcgttgg cgcagcaatc agcgttcagc
      721 aggtcgcaaa acgctggagc ggatttaaca ctctcgctaa acgcgccccc tatttttcca
      781 gtctgttgat tggcttagtc ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc
      841 gataactcaa tcctttaggc ttgcatgtta tcttcagccc aggatgattc ttaaatcagc
      901 tatttccgct gactctctgc tcgctaagga tgcttttagg gcatcctttc atttacactt
      961 tttacgaaat catgggatca ctaacaaaat atcgcttgtc agttatattg tatggcagga
     1021 aagatatgcg actgatatta cagatcccca aagtggagag tttatgacca ttaaaaataa
     1081 gatgttgctg ggtgcgcttt tgctggttac cagtgccgcc tgggccgcac cagccaccgc
     1141 gggttcgacc aatacctcgg gaatttctaa gtatgagtta agcagtttca ttgctgactt
     1201 taagcatttc aaaccaggcg ataccgtccc agagatgtac cgcaccgatg agtacaacat
     1261 taagcagtgg cagttacgta acctgcccgc gcctgatgcc gggacgcact ggacctatat
     1321 gggtggcgcg tacgtgttga tcagcgacac cgacggtaaa atcattaaag cctacgacgg
     1381 tgagattttt tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa tgcagacacc
     1441 ttgttatttt ttattattag ccacttgctc gtcttgcttg ttattagtcg tatttcacgt
     1501 tgattaatgc ggttgcctcc agtgcgccag atttaacttt gtttgtatcg tagacgtagt
     1561 aactggcagt tatcggaatt gaaaccgttg tatctgtaag ttttgacttt gccgcaattt
     1621 cttttaaatt tgtgtatcct ttactgctgt tagtgatatt taaaagtgtg actgacgaac
     1681 cattactttc aagaataaat cctacgccat tatcttccgc acctttcagt attgtgttgc
     1741 tgttggttaa caagtcagaa cttgataaat acacctgcat tttccgtgat gtttgatttt
     1801 gttgacctaa taaattttta caacgcagaa taatatcccc ttgtttacta tttattgttg
     1861 ctttgttacc tgtggctggt aattgtgaaa cgggaatact gtctaaagta attaataaat
     1921 cgtccggttt gcaggttgtg gttatcgggt tgtatgacaa tgtcagatta aaggaataga
     1981 cacctgcatt ttgtgccaat gcatttctgt agctatttac acatgcatcc catgacttgc
     2041 cacctaataa acacagtaga aattgtactg cggcactcct gcgtgtcgat gatgaagcac
     2101 tggataatgt cgcaactcca ttaatcatca ctgaaccgcc gccccccgca gaaacattca
     2161 cttttgcatt actggccgca ggtgtcaccg ttaccgaata accactggtg aaactggtct
     2221 gattatttga taacgtgatg ttgtttttat tggtccattc aaaatgtaaa tttaatttaa
     2281 tggtatcgtt ataaagtcca acaataatat ccgacgggct cataggggtg atagtatttg
     2341 tactcacgca tgacgaatca ttaaaattat gctcatatgt cgccatttgt gtttgagtaa
     2401 gtgcaaaatc cccctgaaca accaaattgt gctgaacaga taactcacta taacatgccg
     2461 catagctcac aggcataacc atcatcatta ctataaataa cattatacga atattcataa
     2521 ccacctgact attactggca aatatagttt ctactttcat caatctcttt accgaaggtg
     2581 attgtgcatg aaagtccttg ttgcttatca attgctacat taaccgatgg cggtacttca
     2641 ttggtgcgaa taaataactg gcttccctga ccgacaacgc caatattatg accatggata
     2701 tcattgactt cataaccaaa cattaatggt tgcccatccg ctcttaacgc ttttataaac
     2761 cagggcttgc gctgatcggt atcaaaatta accagtacaa ccgcgccgcg ataaggggcg
     2821 gcaattttcc ggttgccacg taattctgct tcgctatcgc tttgcgacac atccagcatc
     2881 aggtgatttt cccgataagg tgtcattccg tcgtatacca ccactccatt acggtttgtt
     2941 gtgcgatatt tttgcccatt gacataagca tctttaattc ctggcgcatt catcacagca
     3001 aacgtttcgg aaagacggtt cgccagatta acgccccccg accaggcgac aatgccccct
     3061 gaaacactgg ctccagcctg tcgataagta ctcgactgac tataactgcc attcactgtc
     3121 gcaaccggcg cgttccaggt caaattcgcc ccagctgtcg tttcatttcc ctgattctga
     3181 taactcaggt tgaccccata gttaaactga tcccggcttc caacggttcc tgataatccc
     3241 gtattatttg aggcaaaccc ctgatcatca aacgtcgttg agttagacat atatatttgc
     3301 cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa tcgatataaa aatattaaaa
     3361 cgtttctctt catgatgatt ctcgtcatac gcctggcttg ccgcgaggat atagcttatc
     3421 cgccgccagt tattggaata actcaactga taatccttac tgctgccgct acgcccccag
     3481 taatctcgcc ataacgtact taaggacaca gacccccagc cttctggcaa tgactggctc
     3541 atattggcag agaagctatt tttacggcca aaatcgttct ggtaataatc ggcaatgtca
     3601 tagacatcgt tttcatcacg gcgataatta tctttattgt ttgcccaaac gtgatcatta
     3661 aatgtccggt aatcacgcga tgaataacgc caggccgcca gaccaaaacg cgtcgacgtt
     3721 tggctcacaa atttgttgta ggcaatttga taactttgcc cgtcaaacac atcgccgttg
     3781 tcttgtttac tatgcgactt cgtggcatcg acggaaatgg caccaaagcg tgtattccag
     3841 ccagtcccca aagtaaacgc gtaataatta ttcgcgacca tcgagccacc atacagcgtc
     3901 aataaattat taaaaccata ctgataaccc gcctggacaa aatcactttg tttgctcgcc
     3961 ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg atacgccggg ttgcagcata
     4021 tttggcactg ctgcataagg caccagatag gtggttaccg agccgtccgc ctctttcacg
     4081 ctgacatcaa gatctgctcc accaccggcc aactgcaaat ctgtaatcgc gaacgggcca
     4141 ggaggaacct ctttctgata aaccacaaaa ccgttctgtt caatagttac cagcgcatta
     4201 ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt tcgagttagg caacatctgc
     4261 atatcacgaa acaaccgcac accgctgaag cgaacagaat caaaaatatc gcttgatgtg
     4321 tacatatcac ccacgcgaag cgtgccgaga agttgggcaa atccacgttc cagatacagg
     4381 gtattgcttt tccacacccc tggattgtta tttgttttac tgaaactggc atcagaatgc
     4441 agttgccacc ccagtaaatt taacccgctg ttaaaacgta catatgtact cttactatta
     4501 cccgacgctt tatagtcact gtaatactga ctcacataat aagaggtata aaacgcatta
     4561 ataccccgct cccagttttc cggtggaaca tagccacttt ccagttcttc cacccaggcc
     4621 tgcgggacac tgaaatcgag acgaaaaacc ccgatatccc aggtatagct cccaccctga
     4681 acaagttgct caaatgttaa acattgctta ccgctggcga agttatcgct attaatgcct
     4741 aaccgcttga taacttctat tgataaacat gtttcttgcg ggttgtcttt aacaataatc
     4801 tcatatttcc cgcgccattg cttattg
//
LOCUS       DAFAVF010000233         4777 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFAVF010000233 DAFAVF010000000
VERSION     DAFAVF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.235"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..596)
                     /locus_tag="LGK42_004253"
     CDS             complement(<1..596)
                     /locus_tag="LGK42_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK2660422.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="LGK42_004254"
     tRNA            complement(711..786)
                     /locus_tag="LGK42_004254"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="LGK42_004255"
     tRNA            complement(793..867)
                     /locus_tag="LGK42_004255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="LGK42_004256"
     tRNA            complement(984..1068)
                     /locus_tag="LGK42_004256"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="LGK42_004257"
     tRNA            complement(1077..1152)
                     /locus_tag="LGK42_004257"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="LGK42_004258"
                     /gene_synonym="panK"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="LGK42_004258"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK2660423.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="LGK42_004259"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="LGK42_004259"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBK2660424.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="LGK42_004260"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="LGK42_004260"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK2660425.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAILAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1213 a         1215 c         1087 g         1262 t
ORIGIN      
        1 taagaatcaa ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacca acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagcac ctgtttactc
     2041 acatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacatgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgcaaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgtttg
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctaaaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAFAVF010000234         4670 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFAVF010000234 DAFAVF010000000
VERSION     DAFAVF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.236"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(117..878)
                     /gene="kduD"
                     /locus_tag="LGK42_004261"
     CDS             complement(117..878)
                     /gene="kduD"
                     /locus_tag="LGK42_004261"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBK2660426.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(908..1744)
                     /gene="kduI"
                     /locus_tag="LGK42_004262"
     CDS             complement(908..1744)
                     /gene="kduI"
                     /locus_tag="LGK42_004262"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBK2660427.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2031..3212)
                     /locus_tag="LGK42_004263"
     CDS             complement(2031..3212)
                     /locus_tag="LGK42_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBK2660428.1"
                     /translation="MKNVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3467..>4670
                     /locus_tag="LGK42_004264"
     CDS             3467..>4670
                     /locus_tag="LGK42_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBK2660429.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGIL"
BASE COUNT         1210 a         1193 c         1037 g         1230 t
ORIGIN      
        1 ttcataaagt tcattaattg atatggatta tttcataacc atgatatgga ttatgatgat
       61 ctacagatat aaaaaaccct gccatgcggc agggtcataa aagtaagaag aatgaattaa
      121 cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc tgaagcgctg
      181 gaggcaagga acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga
      241 atgcggtcga ggatttccgc gctacgttgt tcatctgccc gtagttgttg agtattgttg
      301 gtcgccatgt aacccggggc tatcgcatta acattaatgt tgtgtttagc ccattcgttc
      361 gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga aggcacacgg
      421 atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc
      481 gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc
      541 atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg aatcaatccg
      601 gcgttattca ccaggatatc aatatgacca aactccgcta ccgcgcgatc cagcagtgct
      661 ggaataccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc
      721 gtgacctgct tgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc
      781 gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacagcc agtgacgacc
      841 gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta ttcgtttatg
      901 cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag acctggtttt
      961 caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa tgaatcgacc
     1021 agctcgggga gatcaccgcc tgctcgttat gcatcacaat atgacgcgtt tcttgcggct
     1081 gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca
     1141 tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacccgga gccagctccg
     1201 tcagccccat actcaattgg caggtttcca gtacatccgg gacaaaatat ttgttaatcg
     1261 tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga
     1321 cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag
     1381 tgccggtatc aatactggca aagacaactt cttttgcacc tttaccaaca tacagggcgt
     1441 cgcggtgacc gatttcatag cattggccat cgacagtaat cgtaccggca ccgccaatat
     1501 tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt
     1561 ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa
     1621 tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat
     1681 tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcact gtggatgctc tgtcttacgt
     1741 ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt
     1801 ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat taaaaataaa
     1861 acaatgtttc atttttaagt taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc
     1921 atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag ccatcattat
     1981 tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag ctattcgtca
     2041 cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc cagtcctttg
     2101 cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg
     2161 cctatcgggt gaccaagtgc gatcgcacca ccattgacat tgacccgacg ctcatcccac
     2221 tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga
     2281 tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc
     2341 gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcgaat gcgggccagc
     2401 acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca
     2461 tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa tgaaggattt
     2521 aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt atcaacaacc
     2581 aacgtctgac cgttactttg ggtcattacc gggacgatct catctttaaa tcgtccggcg
     2641 tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caattgacgg
     2701 ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag
     2761 gcatcccata acccatcatg cacaagactg tcaaccagct ggctattgcc aagctgtgca
     2821 ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccaccggcg
     2881 atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaagt
     2941 ccggaaccgc agacgtcatt aatcgtgatt gcagaaacgc tattaggcag accaccttta
     3001 atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt
     3061 acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga
     3121 ctaccaagtt ccacggcgga atgacccgct aacgcaccgc gaaagcagcc gataggtgtc
     3181 cgtaacgccc cgacaatcac aacgtttttc atcacaacct cgcaccaatt aacagtgcaa
     3241 tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca
     3301 aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact
     3361 aacccaccaa tagaggggta gatagggcgt taatctccca tacttaacct ggtttatggt
     3421 aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg
     3481 gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg gtgcaggcac
     3541 gctcttcctt cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct
     3601 ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca tcctctcatc
     3661 gaaaacatca gcaggtgaag ggataacggg cgcagtaaca cactactatg gcaagaagat
     3721 tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc
     3781 agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg
     3841 catccgtatg ttggtgagtc tgggtgttgt attaatcctg aatctcattt ttctgatggg
     3901 acgccatgca accattcggg taatgggttt tttggtattc ccattgattg cctatttctt
     3961 atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacga cccaggtaga
     4021 gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt
     4081 tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca
     4141 cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt
     4201 actgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg aagcggctaa
     4261 agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggct
     4321 gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta
     4381 ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca acactacagc aggttggtgt
     4441 aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc tgatcacctt
     4501 cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct
     4561 cattgccatg atacttttca tcatgcctac gttgtcaacg tatctgatcc cggcgcttaa
     4621 accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
//
LOCUS       DAFAVF010000235         4638 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFAVF010000235 DAFAVF010000000
VERSION     DAFAVF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.237"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..224
                     /locus_tag="LGK42_004265"
     CDS             <1..224
                     /locus_tag="LGK42_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBK2660430.1"
                     /translation="YIENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQ
                     AVDSNSIPLDYSLLYSNIFEFQVKYFFPR"
     gene            complement(269..1168)
                     /gene="eamA"
                     /locus_tag="LGK42_004266"
     CDS             complement(269..1168)
                     /gene="eamA"
                     /locus_tag="LGK42_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBK2660431.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1363..2550
                     /gene="ydeE"
                     /locus_tag="LGK42_004267"
     CDS             1363..2550
                     /gene="ydeE"
                     /locus_tag="LGK42_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBK2660432.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTISVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            2677..2772
                     /gene="mgtS"
                     /locus_tag="LGK42_004268"
     CDS             2677..2772
                     /gene="mgtS"
                     /locus_tag="LGK42_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBK2660433.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(2991..3881)
                     /gene="dgcZ"
                     /locus_tag="LGK42_004269"
     CDS             complement(2991..3881)
                     /gene="dgcZ"
                     /locus_tag="LGK42_004269"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBK2660434.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4136..4528)
                     /gene="ydeI"
                     /locus_tag="LGK42_004270"
     CDS             complement(4136..4528)
                     /gene="ydeI"
                     /locus_tag="LGK42_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBK2660435.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1251 a         1143 c          978 g         1266 t
ORIGIN      
        1 catatattga gaatgaatgc gggatcaaaa tcattggcac ctttgaaact ttctccccta
       61 cttttcctac cccagaaatc gccagtattt tacggatcag cccacgggat cccatactta
      121 aaattcagac ccaggctgtg gacagtaact ctattccgct ggattattcg ttactttaca
      181 gcaatatttt cgagttccag gtaaagtact ttttcccgcg ataaaagcag acgcacagag
      241 tgctgacatc acattctgtg cgtatggttt agcctctaac ctttaccgct ttacgccacc
      301 gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca
      361 gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca
      421 gtaacgataa cggtgcaacc cgccaggttt catagcgtcc cagtaacgtc ccccagatcc
      481 cataaccaac aattgtcgcc acaaacgcca gatacatcag cgacaagatg gtggtcatat
      541 caatagtaac cagactgtga atcatggatg cggaaccatc gagaatcagc gaggcaacaa
      601 agaagggaat gattgggatt aaagcgctcc agattaccag cgacatcacc gccggacgcg
      661 ttgagtgcga catgatcttt ttattgaaga tgttgccaca cgcccaacta aatgctgcag
      721 ccagggtcaa cataaagccg agcatcgcca catgctgacc gttcagacta tcttcgatta
      781 acaccagtac gccaaaaatc gctaaggcga tccccgccaa ttgtttgcca tgcaatcgct
      841 ccccgaaagt aaacgcgcca agcacgatag taaaaaacgc ctgtgcctgt aacaccagcg
      901 aagccagtcc agcaggcata ccgaagttaa tggcacaaaa aagaaaagca aactgcgcaa
      961 aactgatggt taatccatac cccagcagca aattcagtgg tactttcggt cgtgcgacaa
     1021 aaaagatagc cggaaaagcg accagcataa agcgcaaacc ggccagcatc agcggtggca
     1081 tgttatgaag ccccactttg atgaccacaa aatttagccc ccatacgacc actaccagta
     1141 gcgccaacac cccatctttt cgcgacattc taccgcctct gaatttcatc ttttgtaagc
     1201 aatcaactta gctgaattta cttttcttta acagttgatt cgttagtcgc cggttacgac
     1261 ggcattaatg cgcaaataag tcgctatact tcggattttt gccatgctat ttctttacat
     1321 ctctaaaaca aaacataacg aaacgcactg ccggacagac aaatgaactt atccctacga
     1381 cgctctacca gcgcccttct tgcctcgtcg ttgttattaa ccatcggacg cggcgctacg
     1441 ctgccattta tgaccattta cttgagtcgc cagtacagcc tgagtgtcga tctaatcggt
     1501 tatgcgatga caattgcgct cactattagc gtcgttttta gcctcggttt tggtatcctg
     1561 gcggataagt tcgacaagaa acgctatatg ttactggcaa ttaccgcctt cgccagcggt
     1621 tttattgcca ttcctttagt gaataacgta aagctggtgg tgctcttttt tgccctcatt
     1681 aactgcgcct attctgtttt tgctaccgtg ctgaaagcct ggtttgccga caatctttcg
     1741 tccaccagca aaacgaaaat cttctcaatc aactacacca tgctaaacat tggctggacc
     1801 atcggtccgc cgctcggcac gctgttggtg atgcagagca tcaatctgcc cttctggctg
     1861 gcagctatct gttccgcgtt tcccatgctt ttcattcaaa tttgggtaaa gcgcagcgag
     1921 aaaatcatcg ccacggaaac aggcagtgtc tggtcgccga aagttttatt acaagataaa
     1981 gcactgttgt ggtttacctg ctctggtttt ctggcttctt ttgtaagcgg cgcatttgct
     2041 tcatgcattt cacaatatgt gatggtgatt gctgatgggg attttgccga aaaggtggtc
     2101 gcggttgttc ttccggtgaa tgctgccatg gtggttacgt tgcaatattc cgtgggccgc
     2161 cgacttaacc cggctaacat ccgcgcgctg atgacagcag gcaccctatg tttcgtcatc
     2221 ggtctggtcg gttttatttt ttccggcaac agcctgctat tgtggggtat gtcagctgcg
     2281 gtatttactg tcggtgaaat catttatgcg ccgggcgagt atatgttgat tgaccatatt
     2341 gcgccgccag gaatgaaagc cagctatttt tccgcccagt ctttaggctg gcttggtgcc
     2401 gcgattaacc cattagtgag tggcgtagtg ctaaccagcc tgccgccttc ctcgctgttt
     2461 gtcatcttag cgttggtgat cattgctgcg tgggtgctga tgttaaaagg gattcgtgca
     2521 agaccgtggg ggcagcccgc gctttgttga tttaagtcga acacaataaa aatttaattc
     2581 agccttcgtt taggttacct ctgctaatat ctttctcatt gagatgaaaa ttaaggtaag
     2641 cgaggaaaca caccacacca taaacggagg caaataatgc tgggtaatat gaatgttttt
     2701 atggccgtac tgggaataat tttattttct ggttttctgg ccgcgtattt cagccacaaa
     2761 tgggatgact aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt
     2821 acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc agtaaaccgg cggtgaatgc
     2881 ttgcatggat agatttgtgt tttgctttta cgctaacagg cattttcctg cactgataac
     2941 ggatcgttga cacagtagca tcagttttct caatgaatgt taaacggagc ttaaactcgg
     3001 ttaatcacat tttgttcatc aataaacatg cagcgatttc ttccggtttg cttaccctca
     3061 tacattgccc ggtctgctct tccaatgacc acatccagag gctcttcagg aaatgcgcga
     3121 ctgacacctg ctgtcacggt aatgttgata tgcccttcag aatgcgtgat ggcatggtta
     3181 tcgactaact ggcaaattct gacacctgca cgacatgctt cttcatcatt agccgctttg
     3241 acaataatga taaattcttc gcccccgtag cgataaaccg tttcgtaatc acgcgtccaa
     3301 ctggctaagt aagttgccag ggtgcgtaat actacatcgc cgattaaatg cccgtaggta
     3361 tcattaacca atttaaatcg gtcaatatcc aacaacatta aataaagatt cagaggctca
     3421 gcgttgcgta actgatgatc aaaggattca tcaagaaccc gacgccccgg caatcccgtc
     3481 aaaacatcca tattgctacg gatcgtcagc aaataaattt tgtaatcggt taatgccgca
     3541 gtaaaagaaa gcaacccctc ctgaaaggcg tcgaaatgcg cgtcctgcca gtgattttca
     3601 acaatagcca gcattaattc ccgaccacag ttatgcatat gttgatgggc agaatccatt
     3661 agccgaacgt aaggtaattc atcgttatcg agtggcccca gatgatcaat ccaccgacca
     3721 aactggcaca gtccataaga atggttatcc gttatttctg gcttactggc atctctcgcg
     3781 accacgctgt gaaacatact caccagccac tggtagtggg catcgatagc cttattgaga
     3841 tttaacaaga tggcatcaat ttccgttgtt ttcttgatca ttgccactcc tttttcacag
     3901 ttccttgtgc gcgctattct aacgagagaa aagcaaaatt acgtcaatat tttcatagaa
     3961 atccgaagtt atgagtcatc tctgagataa cattgtgatt taaaacaaaa tcagcggata
     4021 aaaaagtgtt taattctgta aattacctct gcattatcgt aaataaaagg atgacaaata
     4081 gcataaccca ataccctaat ggcccagtag ttcaggccat caggctaatt tatttttatt
     4141 tctgcaaatg agtgacccga acgacggccg gcgcgctttt cttatccaga ctgccgctaa
     4201 tgttgatcat ctggtccggc tgaacttctc gtccatcaaa gacggccgca ggaataacga
     4261 cattaatttc accgctctta tcgcgaaaaa cgtaacggtc ctctcctttg tgagaaatca
     4321 aattaccgcg tagtgaaacc gaagcgccat cgtgcatggt atttgcgaaa tcaacggtca
     4381 ttttttttgc atcatcggtt ccgcgatagc catcttctat tgcatgaggc ggcggtggcg
     4441 ctgcatcctg ttttaaaccg ccctggtcat ctgccaacgc ataaggcatg acaagaaaac
     4501 ttgctaatac aatggcctga aatttcatac tgactcctta attgcgtttg gtttgactta
     4561 ttaagtctgg ttgctatttt tataattgcc aaataagaat actgccaatt gttataaggc
     4621 atttaaaatc aggcaact
//
LOCUS       DAFAVF010000236         4603 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFAVF010000236 DAFAVF010000000
VERSION     DAFAVF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.238"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..246
                     /locus_tag="LGK42_004271"
     CDS             <1..246
                     /locus_tag="LGK42_004271"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK2660436.1"
                     /translation="FIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQAGRPYNEGE
                     QVVIGGNERYISVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(548..3223)
                     /gene="adhE"
                     /locus_tag="LGK42_004272"
                     /gene_synonym="adhC"
     CDS             complement(548..3223)
                     /gene="adhE"
                     /locus_tag="LGK42_004272"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK2660437.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3700..4347
                     /gene="ychE"
                     /locus_tag="LGK42_004273"
     CDS             3700..4347
                     /gene="ychE"
                     /locus_tag="LGK42_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBK2660438.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         1141 a         1058 c         1126 g         1278 t
ORIGIN      
        1 tttattggca gccaatactt actggcatgg tccgacaaac tggttgaatt aaaaaccatc
       61 tgtttttgtg gccgtaaagc aagcatggtg ctgcgtcttg atcaagcagg cagaccttat
      121 aacgaaggtg agcaggtggt aattggtggt aatgaacgat acatttctgt atgccgtaaa
      181 cactataaag aggcgttaca agtcggctca ttaacggcta ttcaggaaag gcatcgccac
      241 gattaataag aatttcttta ctgacagggt gagcagggca cttttatcct gtcagttcgt
      301 tttacgcact tcttccgggc tatataccct tctcggcagt tttttaacgc cgctatacgc
      361 ctcacagggc tcttaagcac cgacgttgac ttgtgacctg taaagtacaa tatccctgtg
      421 tttaggcgtt atacatcgtc gcaaatatga tgaaggctaa tgctgtcggt ttatggaaaa
      481 gttgctttgg gtaaacaaaa aaacggcccc agaaggggcc gtttatattg ccagacagcg
      541 ctactgatta agcggatttt ttcgcttttt tctcagcttt agccggagcg gcttcttttt
      601 tcgctgcagt ttcaccttct acataatcac gaccgtagta ggtatccagc aggatctgtt
      661 tcagctcgga gatcagcggg taacgcgggt tagcgccggt gcactggtca tcgaacgcat
      721 cttcagacag tttatccacg ttcgccagga agtctgcttc ctgaacgcca gcttcacgga
      781 tagatttcgg aatacccagt tcagctttca gcgtttccag ccatgccagc agtttctcga
      841 tcttagcagc agtacggtcg cccggtgcgc tcagacccag gtggtcggca atttcagcat
      901 aacgacggcg agcctgcgga cggtcatact ggctgaatgc agtctgcttg gtcgggttgt
      961 cgttcgcatt gtagcgaata acgttacaaa tcagcagggc gtttgccaga ccgtgcggaa
     1021 tatggaactg ggaacccagt ttgtgcgcca ttgagtgaca tacacccagg aaggcgttcg
     1081 caaacgcgat acccgcgata gtcgctgcac tgtgaacacg ttcacgcgct accggatttt
     1141 tagacccttc gtggtaggac gctggcagat attctttcag cagtttcagt gcctgcagag
     1201 cctgaccatc agagaactca gatgccagta cagaaacata agcttccatg gcgtgagtta
     1261 ctgcgtccag accaccgaaa gcacacaggg acttcggcat gtccataacc aggttggcgt
     1321 cgacaatcgc catatccgga gtcagcgcat agtctgccag cggatatttc tgaccagtag
     1381 cgtcgtcagt tacaaccgca aacggagtga cttcagaacc tgtaccagaa gtggtggtga
     1441 cagcgatcat tttcgctttc acgcccattt tcgggaactt gtagatacgt ttacggatat
     1501 ccataaagcg cagcgccagc tcttcgaagt gagtttccgg atgttcgtac ataacccaca
     1561 tgatcttcgc ggcgtccatc ggggaaccac cacccagcgc gataatcacg tctggtttga
     1621 aggagtttgc cagttctgca cctttacgaa cgatgctcag ggtcgggtcc gcttctactt
     1681 cgaagaagac ttcagtttca acgcctgctg ctttcagtac ggaagtgatc tgatcagcat
     1741 aaccattgtt gaacaggaag cggtcagtca cgatgagcgc acgtttgtgg ccatcagtaa
     1801 tcacttcatc cagcgcgatt ggcagggagc cacggcggaa gtagatagat ttcggaagtt
     1861 tgtgccacaa catgttttca gctcgcttag caacggtttt cttgttgatc aggtgtttcg
     1921 gaccaacgtt ttcagagatg gagttaccac cccaagaacc acaacccaga gtcagggaag
     1981 gtgcgagttt gaagttatac aggtcaccga taccaccctg agacgctggg gtgttaatca
     2041 ggatacgagc cgttttcatt ttctgaccga agtaagaaac gcgagccggt tggttatcct
     2101 ggtcagtgta caggcaagag gtatgaccga taccgcccat agcaaccagt ttctctgctt
     2161 tttctaccgc gtcttcgaaa tctttagcgc ggtacattgc cagagtcggg gacagttttt
     2221 catgtgcgaa cggttcgctt tcatcaacaa cggtcacttc accgatcaga atcttggtgt
     2281 tttctggtac agagaagcct gccagttcag caattttata ggctggctga ccaacgatag
     2341 ccgcgttcag cgcaccgttt ttcaggataa catcctgaac agctttcagc tctttaccct
     2401 gcaacagata gccgccgtgg gttgcaaaac gttcacgtac agcgtcataa acagagtcaa
     2461 caacaacaac agactgttca gaagcacaga ttacgccgtt gtcgaaggtt ttggacatca
     2521 gtacagatgc aactgcacgt ttgatatcag cagtttcatc gataacaact ggagtgttgc
     2581 ccgcgcctac accgatagct ggtttaccgg agctgtatgc ggctttaacc atgcccggac
     2641 caccagtcgc gaggatcagg ttgatgtctg ggtggtgcat cagtgcgtta gacagttcaa
     2701 cagaaggttg atcgatccag ccgatcagat ctttcggagc accggcagcg atagcagcct
     2761 gcagaacgat atcagccgct ttgttggtgg catcttttgc acgcgggtgc ggggagaaga
     2821 taatggcgtt acgggtcttc agactgatca gcgatttgaa gatagcagtt gaagtcgggt
     2881 tagtggtcgg aacgataccg caaataatac cgattggttc agcgatagtg atggtaccaa
     2941 aagtgtcgtc ttcagacaga acaccacagg ttttttcatc tttataggcg ttgtagatat
     3001 attcagaagc aaagtggttt ttgatcactt tatcttcgac gatacccatg ccggattcgg
     3061 caacggccat tttcgcgagt gggattcgag catctgcagc agccagagcg gcggcgcgga
     3121 agattttgtc tacttgctct tgagtgaaac tggcatattc acgctgggct ttttttacac
     3181 gctctacgag tgcgttaagt tcagcgacat tagtaacagc cataatgctc tcctgataat
     3241 gttaaacttt tttagtaaat catctgctcg aatacgagag tatagtcagt gcggtgatga
     3301 tttgcttaac ctatgaaaat caaaagctta ctcgcgctca cactcactgt gatttactaa
     3361 aagagtttaa acattagagt tattatctct aatgcgtcac ttccaggtgg cgtaagcaag
     3421 attactcact tctgggtact gattacgtga tccaaatcaa atttttgcaa agctgacacc
     3481 tttcagcatc gcttttcgcc attatagcta acagttaata aattgtagta tgatttggtg
     3541 gctacattag catgttttgc acaactagat aacaataacg aatgatagca attttaagta
     3601 gttaggaggt gaaaaatgct gtcaaaaggc gtattgtcag cgcgtctttt caaccttatt
     3661 tatggctaac attatccggc ttttgcttcg gagctaaccg tgattcagac cttttttgat
     3721 tttcccgttt acttcaaatt tttcatcggg ttatttgcgc tggtcaaccc ggtagggatt
     3781 attcccgtct ttatcagcat gaccagttat cagacagcgg cagcgcgaaa caaaactaac
     3841 cttacagcca acctgtctgt ggccattatc ttgtggatct cgctttttct cggcgacacg
     3901 attctgcaac tttttggtat atcaattgat tcgttccgta tcgccggggg catcctggtg
     3961 gtgacgatag cgatgtcgat gatcagcggc aagcttggcg aggataaaca gaacaagcaa
     4021 gaaaaatcag aaaccgcggt acgtgaaagc attggtgtgg tgccattggc gttgccgttg
     4081 atggcggggc caggggcgat cagttctacc atcgtctggg gtacgcgtta tcacagcatt
     4141 agctatctgt ttggtttctt tgtggctatt gcattgttcg ctttatgttg ctggggattg
     4201 ttccgcatgg caccgtggct ggtacgggtt ttacgccaga ccggcatcaa cgtgattacg
     4261 cgtattatgg ggctattgct gatggcattg gggattgaat ttatcgttac tggtattaag
     4321 gggattttcc ccggcctgct taattaattc ctttcaaatg aaacggagct gccatgctcc
     4381 gtttacttcg tcattatttt tactttgttc ccgcgcagtt atcaaaagca aaaggaatag
     4441 gtaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg cttctcctca
     4501 ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa ttgaaatgat
     4561 attagtaatt ccactgcctt tggtagagga aagtgctaaa taa
//
LOCUS       DAFAVF010000237         4536 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFAVF010000237 DAFAVF010000000
VERSION     DAFAVF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.241"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(64..3183)
                     /gene="ag43"
                     /locus_tag="LGK42_004274"
     CDS             complement(64..3183)
                     /gene="ag43"
                     /locus_tag="LGK42_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBK2660439.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVHSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHGS
                     AQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(3555..4427)
                     /locus_tag="LGK42_004275"
     CDS             complement(3555..4427)
                     /locus_tag="LGK42_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBK2660440.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          908 a         1341 c         1246 g         1041 t
ORIGIN      
        1 atccgttgcg ccggtccggg tcccgatcat gatgaccggg accacagaga ggcgatggtt
       61 ctgtcagaag gtcacattca gcgtggcctg accgttatag ccttcagcgc tgctgccgct
      121 gacgctgtgg acataacctg cctgaacgcc cagggtgata ttttcccgga cacgggcttc
      181 cagtccggcc tgcaggtcca gtgacgtacc attctgtgac ggtgagaacg tcatgttact
      241 gccggctgcg gctgtaccca tgctcatgtc cccccgggag ctgaaggtgc ggataacaga
      301 aggctgtacc caccagttca ccggcagttc acgcacactg tgtttggcac tgccgcgcag
      361 ggtgtcacgg gatgaggtgc ctttaccaaa gttcatatcg tggtggctgc ccagacggaa
      421 gccggcacgc acatgttgtg cactgccatg cccgaacttc acataactgg cgttatcctg
      481 gccgtcatcc agggagagtc cctgccaggt gtactgcagt tgcggctcca gcatcagatt
      541 gtcagtgata ctgaagggca gaccggtttc cagcgaaccc agccagcccc agccccggac
      601 gcggaagtcg ttattgtccg atgaggcttt catgctgtgg cgggttccct gggccacaat
      661 gtcagcccac aggccggagg cgttgtgtat cagattcagg tatccgccca ggctgccggc
      721 atcatcccgg accgtgccgg cacgggagcc gtcatcatcc ttaacatcaa cggaagaatg
      781 gccagcagca ccatataccc ccgcggtcac agacataccg gcaacctctg ttctgagcag
      841 gtcaccctcc agacggacga agccatagct gccgctgctt tccggcgtgg ctccacgggc
      901 aataccgccg ttgttatcgt gaccgagatg accgccctga atgctgagac ggacgctgtt
      961 attttcaccg cttacaccgg tctgatggct gcgggagcct gccagaatcc ggtcatagtc
     1021 cattgcctgt gtcagcatgg aggcatacag ggggacttct gcacgataag cattttcact
     1081 gcgcagatac cagctctcat cactgtcccg gttgagggag tagttaaagg caccggcctg
     1141 cagcttattc ccctggataa aggccccttc ctccgtggtg gcaccgttaa tggcttccac
     1201 cacctgaata cccttaccgc tggtcgccag ccccgacgca ctgttgccgg cgttcaccag
     1261 gttcaggatg gtttttccgg ttgccctgcc accgtcaatg accagtctgt cagcattgtt
     1321 ctgtgccata tccgggcgta cacgcaggct gatggtgcca ttctgtccgt tcaggttttt
     1381 cactttcagg gttgccggta cgaacttccc tgtgcgggtg gaggtgaaat gaatctgtcc
     1441 ggcatggctg aggtcatcca ccaccgactg caccgtggcg ttatcgggaa tattccaggt
     1501 ggcaccagag gcgagagtga cattcgtggg gtcaatggca ccgttcagca cagtgctgcc
     1561 ggtcagcttc agggctgtac cgcgctgagc aatgacatcc gtggtgacgg tactgtcgtt
     1621 cagcgtcagc gtgccttcat tcaggttgac ggctttctgg gtgagtgtgg tgttgctgac
     1681 agtgagtgtg ccacttcctg atttttccac gctgccgtta ccggtgagag agcctccctg
     1741 caggagtgca tcgccttcac ggatggtcag ggtatcgttg ttcaccgtct tcccggaaag
     1801 ggtaagtatg gcgccgttat tcagcgtggt ggtgcccgcc agtgtgccgc ccctggcggt
     1861 gaacagtccg ccatttaccg tggtatccgt ggccgtatca ccggcgttca gcgtgaatga
     1921 actgcctttt tccagcatca gggcatccgc ctgaccacct ccgatactga atgcctttcc
     1981 gtcgctccgg gtaccactga cagcggcacc ggaatcggcc agcagtacac cgccatttcc
     2041 catggatacg gtggtggcgg tgccaccgtt gcggacatcc agcgttccgc catcatccac
     2101 gcgggtatta gtggctgtgt gtccggtcag cacatccagg cgtccgccat tttccagtac
     2161 gacattatca gctttaccct ccacaacaga gaatgctccc aggcggttta tgccggtaac
     2221 ggttgcagcc gtactggtaa ccagtgcgcc gccctgcttc agggtgacat tcgtggctgt
     2281 accaccggcg tccacctgca gtctgccctt ctgattaacg gtggtattcc cggccacacc
     2341 cccgtttttg acaatctgcc agccgtcact gtgtacaaca gtgccagacg ccgtaccgcc
     2401 gttgtgcaca tactggtacc ccccattcag cgtggtatcc agtgcgtgac catgtacagt
     2461 ctggtcgccg ccggcataaa ccacagtggt atttgccgtt ccttcagcag ccacaatctg
     2521 acgaccattt ttattgatgg tggtgcgtac ggcatctccg tagacggtct gcccggtatc
     2581 accattttcc gcatccggtc ccccttccgc gccggtattc acaaccgtgt cggttgccac
     2641 tgcgccggat ttgatggcct gccagccctt ctcattaatg acggtacccg ttgcaatccc
     2701 gccttcatgt acccactgct caccgccgtt cagagtggtg ttcactgcct gcccctgaag
     2761 gctctgtccg cctccggcac tgataaccgt gtctgaaacg cttcctccgg cattcactct
     2821 ctgaagacca ccaccggtga cagtagtgtt gttggcgata ccgccatttt gtatccattg
     2881 tccgccggta ttggcctcgt tatccggccc atactccaga ccggtactga tggtcattcc
     2941 gttggccgta ccgaggacaa tctggttgtc atgatttgtc agtgttccgt cgttcacggt
     3001 ttctcccgcc tgtacaacct tgtcagcagc cagtgccggg actgatgtga cagcagcaag
     3061 agacagcgca accgccacac cagcgcgttt tccccgtgag cgggccagtt cggaggccac
     3121 caccagggtg cccgtaatgt gattccatac cagcctgtag ctggtgttca gatgtcgttt
     3181 catcagcttt tccttacaga gggtgaataa aaaagccggt acccacaaat gtggataccg
     3241 gcaggtacaa aacagcatgc tcagataaag tggtttttcc cggacataaa tgcttcccct
     3301 ctctcatccg ccagagaagt atcagtccgt tgtgtgcaat tccgttctgc atgatgaaga
     3361 gattcagctg ccttttcgca gaccattaac cactataaac gatcgatagc aacgatcgat
     3421 acctgtttta tcgatcgttt tattctatta acgacaaggc ggtcacgcaa gagaaacagg
     3481 aggagtatga ggggtttgtg tcatgttttc agttggttaa cgtaatgtta tcaggcattt
     3541 ccctggcaga ctgttcagaa gaaaatccag ttccatacag cgcgggcaac agagaccacc
     3601 gtgtcacgga cggcacgcag aaccgtacgg gcaacagagg caacacagac gctctccgcc
     3661 gtgtcaaata tccggtccac cgcaccggta aactgttcac gggcctgagc gcggacagat
     3721 tccgtgcgca gctcgtcctg caggcgggtc atcaggggac tggcggcatg gtcgggaagt
     3781 gctgtcatta gcgcactgac cagcgtatcc agttcccagc cggtgcgggc cgatacggca
     3841 acaaccggat gtacgggccg gaacagacgg aataccgcct ccgttttctc ccgaatgttc
     3901 tgtgcctgtg cgggagaagg ctgaatgccg gccatatccc attcatggca gggctccgtt
     3961 ttgtcggcct gcgtcaccac aaacagcacc tgctgatgtc cccggtgcag gatgtgtcgc
     4021 cagaaatact catccacaga cagggcgcgg tcatcggctt taatcagcca cagcaccagg
     4081 tccagttcgg gcagaatgtc acggtacagg gcttcatact ctgcatccct gtcccggctc
     4141 tcgcccaccc cgggcaggtc agtgataacc atgctgtgac cgtggccact cagacggaag
     4201 cgccgcactt cccgggtgcc ggcgtgaaca tcactgaccg gggtgacctc cccctgaaac
     4261 agtgcattac agagtgagga tttaccggcc ccgcttttac ccataatgcc aatcacgggt
     4321 tcgtgactgg tgagtttgcg cagatgttcc aggatgtgac gggaaagtga gtaaggcagg
     4381 gaggagagcg gtttttcaat tgcctcaatg gcatcagacg gattcataca tgtattcccg
     4441 gtgaaaaaag acaaaacccc gcgcaccgga aaggtggcgg gggattcacg gagataatat
     4501 ctgtctgaaa ttgttttgaa tcgatgggat tgttgc
//
LOCUS       DAFAVF010000238         4457 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFAVF010000238 DAFAVF010000000
VERSION     DAFAVF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.242"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..667)
                     /locus_tag="LGK42_004276"
     CDS             complement(<1..667)
                     /locus_tag="LGK42_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660441.1"
                     /translation="MESARNKYTSEIIEAEDLWLLDVVDTNGYVCWGCGIEMYPSAWQ
                     KGSKKRPSFNKMPGKEHINCDADAESTVVKQGQKKSVRNMLDSAPGLMPAGLKLIEQR
                     PVVDPGTTGSDNNTNPIRSTSSASSGGNAPARQSRRPVNAIRQICRAFIRFPYDRGMS
                     LNIPGINADTYMTVFKKLKDPVQPYPERRVFYSQLLWQKFEQDENRIVIPLSGEWAKD
                     ESGK"
     gene            complement(713..3712)
                     /locus_tag="LGK42_004277"
     CDS             complement(713..3712)
                     /locus_tag="LGK42_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660442.1"
                     /translation="MLDNLLAEGRRAIMILDNCPSELHASLASKVSAAGKEVSLITIE
                     YDIRDDKPQTTEVIHIETDGPDVAEQLLIRRFPSIGQNNARRIAEFADGNARVALAIA
                     ERVEEGESLALLSDAQLFNRLFEQRNHPDGHLREQAEILSLVYSFSISSPDAATDELE
                     ILGVLSGYPKIQLFKAVTKLMERHIVQKRSHWRAILPHAIANKLAASALNSIPIDQLR
                     TTFEAPDRQRLLMSFAHRLGLLHSHAVAKEIVEAWLQPEGLLGKIIDLDDVSTRILNY
                     IAPVAPGALLERIEETLVTSNFEHFKSRYNPQRTAIIKLLQLLAYESKYFERCIKLLI
                     RIADQESASNNYDSARNKIKRFFQPYLSGTHASLEQRVAIMNECIDSSMAERRRMGIE
                     MLETALGGPPWTGFGINEFGARPRDYGFRPSYDELVAWLFAFIDILVRLGTSGVPELE
                     DDARRILADKFRWIWRHGGIRSKLIDAARKLHAHRPWGEGWKAVRSTIYFFHTKRKDN
                     EDVEPLPEILVTLERELEPKDLVSAIMAYVLSNRRDFWALDADYEHQGTNKFSEIRKI
                     LCAKAFKLGHEFAVSSHGLDELCPNLFLRDGMPYRVEFGKGLANGAPDLRIYWQQLVI
                     QLDLQHKSQRDVSVIRGFIEETHSIDSALAQELLDQCAQHSELRFELVNLHPWQEFTE
                     IDLDRCMSLLDDSDIQPHMYGAILWGEQFSNLPESRVLELAQRLLSKPNGDEVVLEAL
                     SMKLADKGDATDTLGLALRTIGISAAIQRFQRDHNDLGGYLDYAMERVIDATLRFDGN
                     EAEKLEWLNTIFAVVDEHFGYIYSFEDAIGITAAWMPKEFLSRIFDGTEDQQQRRLHF
                     INHDDSHQSPIAKIDVDILIEWCRTTKDPQVWASVASGINLWSKDGEQSPICLQDDAL
                     RFLEASPEPRAVLEIFAEHVAPSSWFGSRANVMQPRVEAIGQLVTHERADISKSARAV
                     YEKLTDWVEKEKERERLEDEALEQRFE"
BASE COUNT         1165 a         1085 c          936 g         1271 t
ORIGIN      
        1 acttaccaga ttcgtctttt gcccattctc ccgagagcgg aatgacaatt cggttttcgt
       61 cttgctcaaa tttctgccat aacaactgcg aataaaaaac tcggcgctca gggtagggtt
      121 gaaccgggtc ttttagtttt ttaaacacgg tcatgtaagt atcggcatta atacctggga
      181 tatttaatga cataccacgg tcatacggga agcgaataaa tgccctacaa atctggcgaa
      241 tagcattaac cgggcggcgg gattgccgcg ctggagcatt tcccccgctg gatgctgacg
      301 atgtagaacg tattggattg gtgttgttgt cgctaccagt agtgccagga tcaacaacgg
      361 gtcgttgttc aatcaatttt aaccctgcgg gcatcaaacc cggtgcgctg tctagcatgt
      421 tgcggacgct ttttttctgt ccctgcttaa ccactgtgct ttcagcatct gcatcacagt
      481 taatatgctc ttttccaggc attttattaa acgacggtct tttcttactt cctttttgcc
      541 aagcggaagg atacatctca atcccacatc cccagcatac gtaaccgttc gtatctacaa
      601 catcaagtaa ccaaagatct tctgcctcaa taatctcact ggtatattta ttacgtgcag
      661 attccatgat ttctccttta aacagaattc ctgtgaagac ggcaactagc ctttattcaa
      721 accgttgttc caatgcctca tcttccaatc gctcacgttc tttttccttt tcaacccagt
      781 cagtcagttt ttcataaaca gctctagccg acttagaaat gtctgcacgc tcgtgggtga
      841 ccaactggcc tatcgcttca accctaggtt gcatcacgtt cgcacgacta ccgaaccatg
      901 atgaaggtgc tacgtgttcg gcaaagatct caaggacggc tcgtggctcg ggtgaggctt
      961 ctagaaatct cagagcatca tcctgcaagc aaataggact ttgctccccg tcttttgacc
     1021 atagattaat acctgaagcg actgaagccc aaacctgagg gtctttagtg gtcctgcacc
     1081 attcaatcaa aatatctaca tcgattttcg caataggaga ctgatgtgaa tcatcatgat
     1141 tgatgaaatg cagccgcctc tgttgttgat cctccgtacc atcaaaaatg cgactgagaa
     1201 attcttttgg catccatgca gcggtgatac cgatagcgtc ttcgaaagaa taaatgtagc
     1261 caaaatgctc atcaacaaca gcgaagattg tattcagcca ttctagcttt tcagcctcgt
     1321 tgccatcgaa gcgcaatgtg gcatcgataa cacgttccat tgcataatcc agatatccgc
     1381 cgagatcgtt gtgatctctt tggaacctct ggattgcagc ggatattccg attgttcgta
     1441 gcgcaagacc tagtgtatcc gtagcatctc ctttatcagc tagtttcata ctcagtgctt
     1501 ccaatactac ttcatcacca ttcggtttac tcagcagcct ttgagctagc tcgaggaccc
     1561 tgctttctgg caaattagag aattgctcac cccacagaat tgccccatac atatggggct
     1621 gtatgtcaga gtcatccaga agcgacatgc aacgatccag atcgatttca gtaaactcct
     1681 gccaaggatg aaggttgact aattcgaatc gaagttcgga atgttgtgca cactgatcga
     1741 gcagttcctg cgctaatgct gaatcgatag aatgagtctc ttcaatgaaa cctcgaatga
     1801 ccgaaacatc acgttgagat ttgtgttgta gatccaattg aatgactagt tgttgccaat
     1861 aaattcgtag gtcaggagcc ccgttagcta agcctttacc aaattcgacg cgatacggca
     1921 ttccatcccg caaaaaaaga ttagggcaca actcatcaag tccatgggaa gatactgcaa
     1981 attcgtgccc caacttgaaa gctttggcac aaagtatttt cctgatttca ctgaatttat
     2041 tagttccttg gtgctcataa tcagcatcta gagcccaaaa atcgcgccta ttactgagta
     2101 cataagccat gattgccgaa accaagtctt taggttctaa ttctcgttcc aatgtcacaa
     2161 gtatttccgg caatggttct acgtcttcgt tatcttttct ttttgtatgg aaaaagtaga
     2221 ttgttgagcg gacagctttc cagccttcac cccaagggcg atgggcatgt aacttacgag
     2281 ccgcatcaat taattttgac cttattcctc catgacgcca aatccagcga aatttatcag
     2341 ccaagatccg cctggcatca tcttcaagct cgggcactcc tgaggttcct aatctcacca
     2401 aaatatcgat gaaggcgaat agccacgcaa caagttcatc gtaactgggt cgaaatccat
     2461 aatctcttgg cctagcacca aactcattaa ttccgaatcc tgtccacgga ggtccgccga
     2521 gagcagtttc aagcatttcg atccccatcc ttctacgttc ggccatactt gaatcaatgc
     2581 attcattcat gatcgcaaca cgttgttcta aagaggcatg ggtaccggat agataaggtt
     2641 ggaaaaacct ctttatctta tttcgagccg aatcataatt attgctcgcg ctttcctggt
     2701 ctgcgatacg gattaagagc ttaatgcatc gttcaaagta tttcgattca tatgcaagta
     2761 actgcaacaa tttgataatt gctgttcgtt gtgggttgta gcgtgattta aaatgctcaa
     2821 aattagacgt aacaagcgtt tcttcaatcc tctcaagcaa agctcctggg gcaactggag
     2881 cgatataatt tagaatgcgt gtcgatacat catctaggtc tattattttt ccaagcagcc
     2941 cttcgggctg taaccaagct tcaacaatct ctttagccac agcatggcta tgcaatagtc
     3001 ccaacctgtg cgcgaacgac atcaataatc gttgccgatc cggagcttca aaggtcgttc
     3061 taagttgatc aatcggaata ctattaagtg cagatgctgc caacttgtta gcaatggcat
     3121 ggggaaggat ggctctccag tgggatcttt tctgaacaat gtgacgttcc atcaacttcg
     3181 taacagcttt gaataactgg attttgggat acccagagag cacacccaat atttccagtt
     3241 catcggtagc tgcatctgga ctagatatcg agaacgaata caccagtgac aatatttcag
     3301 cttgctcccg taaatggccg tcgggatgat tacgttgttc aaacaagcga ttaaacaact
     3361 gcgcatctga aagtagggct aaactctcac cttcttccac tctttccgct atagccaaag
     3421 ccactcttgc attcccatct gcgaactctg caatccgacg ggcattattc tgaccaatag
     3481 aagggaagcg acggataaga agttgctctg ccacgtctgg accatctgtc tcaatgtgga
     3541 tgacctcagt tgtctgtggt ttgtcatccc taatgtcgta ctcgatcgtg atgagactta
     3601 cttctttccc tgcggctgat actttgctgg caagcgaagc atggagttct gatgggcaat
     3661 tatcaaggat cataattgct cgccggcctt cagcgagcag gttatcaagc attgctgtag
     3721 ccgacggtac tggctcatag ccagtatcaa cataaatagc tactgttcgg tcaagcgcat
     3781 ctgttccaac agtttcatca aagagcgcct gaacgattcg agtcttccca acaccagaca
     3841 atcctgtaat acgaacagcc ttgtttgtcg aacggatcag tgctcgcatt gggttaattg
     3901 cttcatcaat tttgagtttt tgcccctttc ccgatggtaa agtaatggtt acgcctgggg
     3961 ctgagattaa agtgtctata acaccttgtg gtggattgct ccaggctccg tagggttgcc
     4021 aaccggaata cccttgccca agctttgctt ttacccacag catgactgat ggatgttggc
     4081 gcaaccattg gatgagtttg gagcggtcgt agaaatcaag atgaagataa ctttcgttag
     4141 gatcatcttt tactgcatcc ctcatcgctt tgagacgatc cttctttcct gacggcgagc
     4201 aatcatcacc caggctgaca atgatgtaac tgccttgctt tctggcttgt tcagaaatga
     4261 tgggagaaag tgctttgccg gtgccaattt ccctctcaat tgcagactta ggcatcttat
     4321 gtttctttgc ctgaaagacc gtatcaggtc ttacaagaaa gcctgctttc agttgatcaa
     4381 ttggaacctg aacatggatg tcaattcctc cgtcaggggc tgtaattgaa ccagaccaat
     4441 ttacacatgc tgggcta
//
LOCUS       DAFAVF010000239         4427 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFAVF010000239 DAFAVF010000000
VERSION     DAFAVF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.243"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            424..687
                     /locus_tag="LGK42_004278"
     CDS             424..687
                     /locus_tag="LGK42_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBK2660443.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            605..892
                     /locus_tag="LGK42_004279"
     CDS             605..892
                     /locus_tag="LGK42_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBK2660444.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1334..1867
                     /locus_tag="LGK42_004280"
     CDS             1334..1867
                     /locus_tag="LGK42_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBK2660445.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2121..2804
                     /locus_tag="LGK42_004281"
     CDS             2121..2804
                     /locus_tag="LGK42_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBK2660446.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2977..3528
                     /locus_tag="LGK42_004282"
     CDS             2977..3528
                     /locus_tag="LGK42_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660447.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            3690..3944
                     /locus_tag="LGK42_004283"
     CDS             3690..3944
                     /locus_tag="LGK42_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBK2660448.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            4120..4416
                     /locus_tag="LGK42_004284"
     CDS             4120..4416
                     /locus_tag="LGK42_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HBK2660449.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILASPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
BASE COUNT         1161 a         1113 c          981 g         1172 t
ORIGIN      
        1 ttaaacgatc taaataacct ttaaaaaatc attttgtaga tcattcgatc gtaaatagaa
       61 taattcttag atgataaaac catttgttac acaacccgcc aattgtgtaa caacagcctc
      121 tacaagagca tatttgttca tatgttcgtc agaactgcgg attgtctgga cgagggtcag
      181 acaccgtttc gtgcatatgg accagcatgt tcgaacgcca caaagcagaa gcatcatgcc
      241 tggatcgctt cggcgcggag cgttaagttt tgacgctttt ttgtatgttt tttaagctgt
      301 tagttgactc ggtgcgttat actaagtacc ctttactggt cacaggcaaa actgtgatcg
      361 ggcgtaggaa cccgcgtagt catcgtcggc gcagaagtta atttcttcat ttggcgtcgt
      421 tttttgccat accataaaga caggaacttg tatttcttgt cgtcatctat ggtagctcag
      481 gcggggcagg cattagcctg gccggtttcc gatgacgccg gtattcctac ccccgtctgg
      541 gctatcacca atgagcgtag gaactcggtg atagcgtttt tagttgtcat cggagacctt
      601 tgctatgtca cctcaactga tacagaaact cccggccatc actctgttgg aaggcatgtt
      661 tcccgagcta tcaacaaacc agcttaaagt ctgcgttttt tacgcaatgg gtgttcctta
      721 cgacgccatt gctcagaact gtcggctttc tcccgaaacg gtacgaacct acctgaagcg
      781 aagcctgaag aatttaaatc tggaaggata tgatgcactt cgctctgctg tgctgatgcg
      841 aactttcgtt ttcatgatta gcaatacagc taaagaaaac gaaaaaatgt gaacaggtgt
      901 ccccattaaa ggggacactt tcatggaaat tgcattttgc actttacagg cgatcccaaa
      961 ttgataaaca taaacaccaa gcacaagtta ctttaataaa gcaaatcact tcagacggtg
     1021 tcccagttat ttgtatctgg ctcatgcccg atatatggcg accttactct gaactgatgt
     1081 gctgatgttt tatttcgcgt atcacaatac tgtgccactg tattgctgat attagtgctt
     1141 agccgttctt tatctgtgag ttcgccaaat caccgataca gaacgtacta gagtctgctg
     1201 gttatatgcc tgtcagaact gcagtttatc cagatatggg ctggacactg cttcagacat
     1261 atgatttagc atattcgcct gacatcgcaa aatcgtccat catggatcgg ttttgcgcgg
     1321 agcgttgaac ttcgtgaata tagaacacct gaataaccgg aactggtatc ttgcgcaata
     1381 taatacagcc ggcaaaaaca gagaatcttt attctcatgg ctgaatgagc aaaacgtcgt
     1441 accatggact ccattaataa caagaaagat aagacgggca gactccaggt gctgttaccg
     1501 tgaaagaata ttcgctattt ttccagggta cttttttatt ctggctaatt ttgacattca
     1561 accagtatcg gcattacgtc gccatagcgc cttcattgat ttcgtgaagt ttggcggtga
     1621 aataaaaccc gtcaataaag atattgttga tggattaatg aagatttacc cggaccctgt
     1681 gctaaatccg ggagccaggg aagagctgaa tgcggcttcc agcatatggc tgacaaaagc
     1741 acaatatcag tatctcctca gaatggaaaa tactctccag cctgagtcga ggatatccct
     1801 gctcctggaa ctggtatcaa atgccgaaca tcacggcttt atggagcgcc ttgtaaacat
     1861 cccatagcca gtatttcccg gccttcgggc cgggcttttt tcaattcaag agaatattct
     1921 ggagtgaagc ctgcacggag ccatccccac catggactcc ccctgtcttt tgatggaacc
     1981 gtgtatttct taatggcgaa agaaataaaa ggcttcaccc cagagtattg attcgtttta
     2041 tcaaaggccg gcccgtgcca tcacgtgtgg cggagagaac ggataccggt cttcttttca
     2101 tctcacagta ggacattcgc atgaccacga cagaacatcc cgtgcgccag ttgctctggt
     2161 gtgcactgaa tgccctgaaa acggcacagg aaaaccacaa tataaccagt gaaaccggat
     2221 tacggcatta cctgcttgaa tggctttctg gtgccagcag acatcctgag ttccgtagtc
     2281 tccctggcga aatcatggcg ttaaaagcat tgaccgaaaa agatcgcaat attcccatca
     2341 tcggaacgct gaatacgctc tttctcagct ctgccactgt cggggaatgt ccgttgtttc
     2401 gttttcgggc tgcactgggt aaattacaga aagcaggctg gcgcacatat gtctgcccat
     2461 ggcctgagag ggtgttcaat gaatccattg aacgggcctg ttccgggaaa cgtcatctgc
     2521 tacaactaag tcgcaccgaa gagtgttttc taccgacagg cgatatgagg gcaccagtta
     2581 cgttgcagct gatcactcca ctacaccgca aaactatgca gcttctggaa acggcagagt
     2641 taatattctc cgacgaagga tttcaggtgg tgagaggata cgaacatcaa ttcgggatcg
     2701 aacgcagaga aaccctcttt cacaccctat ttatcgggct gcccagcctg ccagaagatg
     2761 tctggggtgc aacacaggag cagacgacta tcacattaca ctgaggatgt gtaatccccg
     2821 gcataccccg tttccagtct tcgcagcctg taccgccctt cctgccgttt ccgacattct
     2881 ttctgtcact actcgcttgt gccatttttt cgcaagcggc ccccatcgac atgacggtgc
     2941 gagtctttct ttttaccctg agaggaaatt aacagcatga aaaaacaacc actactctgg
     3001 atgacgatag ccgccgcact gctgtccggc tgcacaggcc acagggaaca tcacccgcag
     3061 gtgacattca cggtcgatac cgtgcgaaca gacacaacgg ccagcaaccc aatctgcctg
     3121 gcggaaaaat acggaagcct tcttaatgag cgccatccgg acctgtttgc cggtgctcag
     3181 gggagtattt cacgcagtgc attttttaat gacagcctga cgctgacacc tgaaacgctc
     3241 cacgggatga tacagaatac gccctgtgtt gaatcactgg taacgtccgt aggtccggat
     3301 accttcactg cagatgtctc tcccggtgtc agaatgcagg tatcaccgga tattcacggt
     3361 gcgcccggaa atatcactct gcgactctcg accacagacc agaaacgaaa acagactgtt
     3421 gaacaaagca tttctctcag accggaacag tcccttgtcg tgaatggatt caccggagac
     3481 ggagctggcg aaaagcgcgt cgttctcatc acacctcacg tgcgttaatt cccaacacaa
     3541 cccgtttccc gtcttcgcag cctgtaccgc ccttcctgcc gtttccgaca ttctttctgt
     3601 cactactcgc ttgtgccgtt ttttcgcaac cggtacgtgc agttatgacg gtacagctgc
     3661 ttcttttccc gttccggaag gagtctttga tgccacagca acatcccgga cgactgcaaa
     3721 tactggtcgt cgacgcacac tgcaaacgca ggctgttttc aacgaaaaca ccgacagacc
     3781 ccgatgagct tgcccgacgt ttctgcacgc cggataactg tctcgtggtg gtactacgcg
     3841 acaaccgatt tctgttccga ctggagcgtg ctcccggcag ccactgccgc tggcacaagg
     3901 gcagcagctc ccgccatcag catctacagg actggctgtc gtaaacacaa caaaaacaaa
     3961 agacctcgtc aaaacaggtg ggggccgtgt tctttttagt taaatttacc atcgcgattg
     4021 cgattgtctg tgtacacctt ttaacgattt taatttagcc gtttgttgcc atctttattt
     4081 tctttattac ctctctccct tttatcatgg agattaacga tgtttaccat gttctttcta
     4141 gttaaattaa ccatcgcgat tgtgattgtc tgtggaatcc tttctccgat tttagcttca
     4201 ccacataaca aactgtccac acttttttgg aagataccaa acgcgttctg gattttcctg
     4261 atttgcatat actttgcaat cgccatggcg gtgttaatag aaagcagtga acagacacgc
     4321 atccgccgcc aacaatattt ccaacagtac gaagtgccaa atgagacaca taaaaatgtg
     4381 gtgatagaaa acagtattaa aagtcaacta atatgaaaaa taataac
//
LOCUS       DAFAVF010000240         4418 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFAVF010000240 DAFAVF010000000
VERSION     DAFAVF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.244"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..218)
                     /locus_tag="LGK42_004285"
     CDS             complement(<1..218)
                     /locus_tag="LGK42_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660450.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            447..1055
                     /locus_tag="LGK42_004286"
     CDS             447..1055
                     /locus_tag="LGK42_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBK2660451.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1153..2544
                     /gene="selA"
                     /locus_tag="LGK42_004287"
     CDS             1153..2544
                     /gene="selA"
                     /locus_tag="LGK42_004287"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBK2660452.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2541..4385
                     /gene="selB"
                     /locus_tag="LGK42_004288"
     CDS             2541..4385
                     /gene="selB"
                     /locus_tag="LGK42_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBK2660453.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          996 a         1125 c         1298 g          999 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg
      241 gttatttgtc tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata
      301 tccatcagat agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat
      361 ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat
      421 atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg
      481 tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac
      541 tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc
      601 tggtgaccga agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat
      661 taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca
      721 aaattgaggc actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg
      781 cgcgtccagc ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc
      841 gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca
      901 atctggcgac tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc
      961 caggctggtg tgtcgatcgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg
     1021 aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat
     1081 ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca
     1141 gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat
     1201 cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg
     1261 gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg
     1321 ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg
     1381 cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttggg
     1441 cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg
     1501 accctcgagt acgatctgga cgacgctgga cgcggacatc gcgatcgtgc gctggcgcag
     1561 ttgctgtgcc gcattacggg ggcggaagat gcctgtatcg ttaataacaa tgcggcggcg
     1621 gtgttattga tgttggcggc cactgccagc gggaaagagg tggtggtttc tcgcggcgaa
     1681 ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc
     1741 ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat
     1801 gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc
     1861 aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg
     1921 actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca
     1981 atgccgcagg agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg
     2041 ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa
     2101 agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc
     2161 acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg
     2221 cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc cccccttgcc
     2281 gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt
     2341 ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga
     2401 cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt
     2461 ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg
     2521 tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa
     2581 caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc
     2641 gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg
     2701 gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg
     2761 gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggca cagacccgtg
     2821 agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag
     2881 ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg ccaggtaaag gaggtgctgc
     2941 gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa
     3001 ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg cgagcacgcc agccaacata
     3061 gtttccgcct cgctattgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg
     3121 gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata
     3181 aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc aacagaaacc gccaatgccg
     3241 ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg
     3301 actggctgct tgccgatgtg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa
     3361 cccatacacc gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtca
     3421 cgggacgcgt ttcactgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt
     3481 ggctggcgga taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg
     3541 gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc
     3601 aatggctggc gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg
     3661 aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga
     3721 tgcgcgaatt gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc
     3781 cggttgccgc tcgctggcaa cggaaaattc tcgacacatt agcgacttat catgagcaac
     3841 atcgcgatga acctgggcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag
     3901 atgaagcgct agtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc
     3961 atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca
     4021 tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa
     4081 aagagacggg taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag
     4141 gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg
     4201 ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg
     4261 atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg
     4321 gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa
     4381 aataaggaaa tgattaatta atttttaaaa taaattaa
//
LOCUS       DAFAVF010000241         4378 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFAVF010000241 DAFAVF010000000
VERSION     DAFAVF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.245"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            58..852
                     /gene="hyxR"
                     /locus_tag="LGK42_004289"
     CDS             58..852
                     /gene="hyxR"
                     /locus_tag="LGK42_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HBK2660454.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            1006..1767
                     /gene="hyxA"
                     /locus_tag="LGK42_004290"
     CDS             1006..1767
                     /gene="hyxA"
                     /locus_tag="LGK42_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HBK2660455.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1809..2891
                     /locus_tag="LGK42_004291"
                     /pseudo
     CDS             1809..2891
                     /locus_tag="LGK42_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2913..4106
                     /locus_tag="LGK42_004292"
     CDS             2913..4106
                     /locus_tag="LGK42_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2660456.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1213 a          942 c         1088 g         1135 t
ORIGIN      
        1 acctgacatt ttgaatcaaa atgtcaggtt ttgtagcaat ggcgtggacg agtctgaatg
       61 aagaagcctc tggttattat cagtgcctgt cagttcacca ggctggcact ggagtctctc
      121 atcccagcgg acaggtatat cgtgcgggtg tacagcaacg tcactacaga ggttgaattc
      181 gttttaaaga cctcctgtgg ctacctgttg gctgatatcc cttcgcttcc ttcgggggag
      241 ttagttcttc tggcttgtct tgtccgcttg cggagtgatg ccgggcaatg gcaagtctgc
      301 ctgaccggtg actccgactg gttcgataat gctcctgctt caagtctgta taacggcttc
      361 ccacatatcg gcaccttgct gaaagcgagt cgttatcaga atcaaattat ccgctggtta
      421 ctacgtcctt tacccggcga tatgagtgat ttctggctcc tgactgcacg ggagttaagc
      481 gtgcttaaga ttctgatgca gggcatgagt ttcagtgaga ttgcccgtta tgaacaacgc
      541 agttcaaaga ctctgcatgc gattgccaca caggcattaa ggaaacttgg actgggaaca
      601 ctaagcgatt tcaggctgct ctatacagga tgcggtgaca gcagagtcaa acggaatata
      661 cggctacatg caagatatgt cggacaacat tccgctcatc gactcactca gtatgtgcag
      721 aacatggtgt ccggaattct ttatccagag aaagttatcg ttggtaaatc aataaattct
      781 gacgccatat ccggaaaagt gctttccggg caaagaaaac atatgtctaa aaaaagacca
      841 ggagtgttat gacacagcag cgttgttact accacccact gcggcgcgta cggactcgct
      901 acagtgtagg ggccaaaata tttctactgc tctgggctgc aggagtggga ggcacgctgt
      961 acagcctgta cagcgtgctg gcgtactgac atctgtgagg gaaggatgcg tttcgttctt
     1021 ttctgtccct caggaattgt acctgcacag tttgaagctc tatcaaccgg ttctgtctgc
     1081 aatattacct gcatcagaac agaagaagag ttacgcaata aattgcggca caggccgcag
     1141 agtgtcgtga tatcggcagg gcgacctgcc gaatgcgctg aaatgtggtt ccgattttat
     1201 cgggatcact cattcgtggt ggtgttgtgc gtcgcgccct tttttctgcc accggatgtg
     1261 agtatttctg gcgttctcaa aaacctgagg ttactgaaaa caggaatgtc agtagagcgt
     1321 gctatcagtg ttgcaaatgc ctctggtggg ttttcaggac tgaaacatgc agaaaatttg
     1381 ccagttatgg acagttactc tgtctttatg aatgaggtga ataaccgcac aaagacaatc
     1441 gtcatgtcag aacgcttccc ggaaaagcaa aagaaggtgt tgtcgttgct tcttgctgga
     1501 cacagctggg aatacagtgc gcagtttctc aaaacgggga taagacagat atggcttgct
     1561 gagcagtcgc taaaaaagcg ttggggaata ccggacagca tgtcattgcg ggaagccctg
     1621 ctgctgagca gcaataaatt tcacggtgat ggaaatgcat tggaaaaaac gaatgccatg
     1681 acattgcgtg aaaacggaaa taccaacagg tattccgtgg tggtaaatgc agggacgcag
     1741 gctttatctc tgcataaata taaataagtg ggaaattgaa attagttacc agaatggaga
     1801 atttcttcat gaaaaacagt aaggcatttt accgcagcgc attagcgaca gctattgtta
     1861 tggctctttc tgcaccagca ttcgctgctg ataacgcggt atcaactaca ccggttacac
     1921 tggataaaga taagacaact ctggatcaag atgttgttat tagcaataca gcagaccaac
     1981 agattacagc cgtaacaatt aatgcggcag ataaagatct taatgttact tttggcggtc
     2041 atgatattac tgctgaatca acggcagaca aaaaattcct tgaaggtgta aaagttagcg
     2101 gtgacaaaaa tgttgtgatt aatgctacag gctccaccat cacagctcaa ggtgaaggca
     2161 cctatgtccg gactgcaatg gtcatttctt caactggcga tgttgttgtt aatggcggta
     2221 atttcgttgc aaaaaatgaa aaaagtagtg cgacaggaat atctctggaa ggggccacgg
     2281 gaaataatgt aacgctaaat ggtacaacca taaatgctca aggtaataag agttccagca
     2341 acggctctac ggcaattttt gctcaaaagg gtagcgtatt gaatggtttt aaaggtgatg
     2401 caaccgacaa cattaccctt gctggctcaa atattattaa tggccggatt gaaacaatag
     2461 ttattgccaa ggagaatacg ggaactcata cagtcaatct gaatattaag gatggctcag
     2521 taattggggc ggctaataat aaacaaacaa tttatgcttc tgcttcggca caaggagcag
     2581 gttcagcaac gcaaaattta aatttatctg tcgccgattc aaccatctac tctgatatcc
     2641 atgccctttc tgcaagcgag aattcagccg gtaccacaac aaatgtaaac atgaacgttg
     2701 cccgctctta ctgggaaggt aatgcttata ccttcaatag cggcgataaa gcgggtagta
     2761 atctggatat aaatctttcc gatagctcag tctggaaagg caaagtttca ggggcaggag
     2821 atgccagtgt atctctgcaa aacgggtctg tctggaatgt tacgggatcc tcaactgttg
     2881 atgctctggc agtaaaagac agtacggtta atatcacgaa ggctacagtc aatactggca
     2941 cgtttgcttc tcagaacggc actctgattg ttgatgcctc ttctgaaaac actctggata
     3001 tcagcggaaa agcgagcggt gacttgcgtg tttacagtgc gggttcattg gatcttatca
     3061 atgaacaaac ggcatttatt tctaccggca aagacagcac tctaaaagcc acaggcacaa
     3121 cggaaggtgg tctgtatcaa tatgacctga cacagggagc tgatggtaac ttttatttcg
     3181 taaaaaacac gcataaagca tccaacgcca gctccctgat tcaggcaatg gcagcagctc
     3241 cggctaacgt tgctaatctg caggctgaca cgctctcagc ccgtcaggat gctgtccgtc
     3301 tgagcgaaaa tgacaagggc ggcgtatgga ttcagtactt tggcggtaaa cagaaacata
     3361 ccaccgcggg aaatgcatcc tatgacctgg atgtaaacgg tgtaatgctg ggtggtgata
     3421 cccgcttcat gactgaagat ggtagctggc tggcaggtgt agcgatgtct tctgcgaaag
     3481 gtgacatgac caccatgcag agcaaaggcg acactgaagg ttacagcttc cacgcttacc
     3541 tgagccgcca gtataacaac ggtatcttca ttgatactgc tgcacagttt ggtcactaca
     3601 gcaacacggc agatgttcgc ctgatgaatg gtggcggtac catcaaagct gactttaaca
     3661 ccaatggttt cggtgcgatg gttaaaggcg gttacacatg gaaagacggt aatggcctgt
     3721 ttattcagcc atatgccaaa ctgtctgcgc tgacgctgga aggtgtggat tatcagctca
     3781 acggcgtgga cgttcattct gacagctata actctgtgct gggtgaggcc ggtacgcgcg
     3841 tgggttatga cttcgctgtg ggcaactcga ccgttaaacc ttatctgaat ctggccgcac
     3901 tgaacgaatt ctctgatggc aacaaagtcc gtctgggtga tgagtctgtc aatgccagca
     3961 ttgacggtgc agcattccgc gtgggtgcag gtgtacaggc tgatatcacc aaaaacatgg
     4021 gagcatatgc aagccttgac tacaccaaag gtgacgacat tgagaacccg ctacagggtg
     4081 tagttggtat caatgtgacc tggtaagaat aaagcagtcg gttcccctgt cgactgttat
     4141 accccctgca ggcagcatac tgctgcctgc aggctataaa catccagtat tcatgatgct
     4201 ggttttttgc tgatatttac cccgaacatt cctgatacag actattaata caaccttatc
     4261 tgcgacagat tttattttct ggatatatta tgcgtgaaca aatcaaacag gatatcgatc
     4321 tgattgagat tttattttat ctgaagaaaa agattcgcgt tatccttttt attatagc
//
LOCUS       DAFAVF010000242         4094 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFAVF010000242 DAFAVF010000000
VERSION     DAFAVF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.246"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(268..417)
                     /locus_tag="LGK42_004293"
                     /pseudo
     CDS             complement(268..417)
                     /locus_tag="LGK42_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            456..1284
                     /locus_tag="LGK42_004294"
                     /pseudo
     CDS             456..1284
                     /locus_tag="LGK42_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            1284..2300
                     /locus_tag="LGK42_004295"
     CDS             1284..2300
                     /locus_tag="LGK42_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK2660457.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVSPDNVRQLREI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            2302..2571
                     /locus_tag="LGK42_004296"
     CDS             2302..2571
                     /locus_tag="LGK42_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HBK2660458.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            2568..3509
                     /locus_tag="LGK42_004297"
                     /pseudo
     CDS             2568..3509
                     /locus_tag="LGK42_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(3544..>4094)
                     /locus_tag="LGK42_004298"
     CDS             complement(3544..>4094)
                     /locus_tag="LGK42_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBK2660459.1"
                     /translation="KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVAL
                     RELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQ
                     RYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDY
                     VWRQSRRLEDGKFRPLRMPGKP"
BASE COUNT         1052 a         1016 c         1009 g         1017 t
ORIGIN      
        1 tagcatgccg gacctttatc ttcagacaag aaaaaaacag cagcaagagc aataatcgtt
       61 gaaataaaaa taaatttcat gctaattact ttcataaaat ctccacaact tctgtcgttc
      121 attcgatata aaaaaacatt acgaatatat ccgtacaatg tgagcatact gtgaagtggc
      181 tttaacaatt atgaaataat acaagagatc acatattgca gtggtctctt ttttatagaa
      241 gaaatcacaa ttcataattg tgatttatta tgcatgtctg tttttcagca ttgtgaccgc
      301 atcggactca ggcatcaaaa actgcacccg gtgccgtgca gccacatcca gtgcaaacac
      361 cttcgtatag acctctgttg agctgatgga cttatgcccc atcaggctct gcaggatgtc
      421 gctctaggag caaaacatcg ttatcgaggt actgcatgac tattttaaat aaaaactggc
      481 ccgtcgaatt cccgttcccg acagtaatcc gcactgtggt gtgcagcgat cttgatgaaa
      541 cttatattcc ttcggacagt gataaaaaaa actagggggg gcgttcagct ggagtcctat
      601 attacatccc atgctgaaaa aaaggggatt ctcgctggct ggatcacggg aacaaatctg
      661 gattcagcct ggcgaaaatc caggggatat atctcgcgaa gccctcactt tatatgttgc
      721 agtctcggca ccgaatttta ctgggtgaaa aatggccgtc tcatcccctc tgaaacctgg
      781 gcagaaagaa taagccgttc aggctacagc cgtcagaatg tcaactgtct ggttaagatt
      841 ctgatggaaa aagagatccc ccttctgaaa caaccagagg attatcaggg cccctttaaa
      901 gtgagctatt actaccctga gagcccatct attgccagcg attttgccac cattcaggaa
      961 ctggctgacg aacgacgaat tcgtgttgtg tttacccgct gtaacccggc ggctggcgat
     1021 ccccctggct gttatgacgt tgaatttatc cccctctgtt gtggcaagga tcaggctgtg
     1081 tcttttctga tggaagaaac cggactgccg aaagaagcca ttattgcctt tggtgacagt
     1141 gcgaatgatt ttgccatgtt tgcacaggcc ggaagtggct atctggtggg taatgccgac
     1201 ccgtttgcag tcagccagta cggaaacaga ctggacagtg cttactgcca cgggatactg
     1261 agcgttctta aggagctaca gtgatgatta tcggaattgt cggctgtggg gggattgccc
     1321 gtgcccatgt caacgcactt tgtctcaatc cgctagtaac tggtctggcc ctgtacgatg
     1381 tgagtccgga caacgtccga cagctcagag aaataagctc cctgccggta acaaactgcc
     1441 aggatctgga gcagcttgcc ggagtcagtc aagggttcat tatctgcaca ccaaaccatc
     1501 tccacgttag cgtggcagaa gaagtgcttc gctacaaaca gctcccgttt gtgtgtgaaa
     1561 aacccctctc aacagatctc aactctgcac gatatctaca ggagattgct ccaaaaggca
     1621 gtatcgtgtc ttttaactac cgatataacc ccgtcatcga catgataatg cgtctgagaa
     1681 atgagaggaa attaggtgaa ctgcatttct tctcagcaga atttaataaa aattctgcac
     1741 tgacccgcca tcacctgacc tggcgagact ccgcacaaca gagcaaaagc agcggtgctc
     1801 tgggcgatct atcctgtcat ctgcttgatt tattttgttt tataggggaa agcccggtag
     1861 tggttcatgg gatcaaaacg gtaaagggga cacgggtggg tacaaaatca gatggtcagg
     1921 ttgaagtgga tgataacggc tatgtaatgg gcagttcaga aaagggagct tatttcagag
     1981 ttcatgccag taaatcggaa acagatcaga atttgggact gcatatacag cttgtatttg
     2041 aaaatggtga aatcagatat tcaacccacc atgaaaatcg cctgctcctg attctattta
     2101 atgatacgaa tacggaaaca attggcttcg acgctctgaa acgcttgcct gatccaccac
     2161 gggagcttcc attctggtct gattcgttta ttcatcttca tgacgactgg tgtgctctta
     2221 ttaaatatgg tcactcgtct cctaagctgg cggacctgtc atccggcctt catatacagg
     2281 aaataattga ggcattctga tatggatacc attcttttaa ccgggctgtt tgctgcattt
     2341 ttcacaacgt ttgcgttcgc tccccagagc ataaaaacta tcaggacacg caataccgaa
     2401 ggtatctccg ttgttatgta tatcatgttc ctgacaggtg taatctcatg gatagcgtat
     2461 ggcattatgc gatctgattt tgccgtactg atcgccaata ttgtcacact gtttctggct
     2521 gcaccagtgc ttgtaataac acttatcaac cgcaggaaaa aacacgtatg aatattgcaa
     2581 tcatcggcgc ggatcctgcc ggcatcattt ctgcccgtaa tgcgattaaa gcaggtcact
     2641 ccgtggttct gtttgagaag aatacccgaa ttggggggat ctggaacccc tggagtggtg
     2701 gcgcttaccg taatgcctgt atgcagaact cccgttatac atttcattat actggctttc
     2761 cccctggcga tatcgatgaa tttccaggag tggaacaggt attcagatat ctttcggccg
     2821 tggccggaga ggatgccctc cgtgagtcga cccgactgaa tactgaagtt gtttcactca
     2881 gaaaagacgc cggacactgg gtgatccgct gcgcttctga aggaaaagac acggaagaca
     2941 tttttgaccg ggtcatcatt gcaacaggtg aactctggca accccgccgc ccccccctgc
     3001 caggtgagga aaacttctcc ggaacgttga tcacgtcgag agattatcag gagccagagg
     3061 catttaaagg aaaaaatatc ctcatcattg gcggcggtgt cagcggtgcg gatattgcct
     3121 cagaccttgt tccctttgcc agaagcgtaa gtctgtccgt caaaaagatg ggactttatc
     3181 tgccgagaca attcccgact ggcccgaatg acatgatgca ctcctatctg ggcaggtgtc
     3241 tgctgagcca aatgaattat gaagatttta tcggttatct cgacaccatg atgcctgact
     3301 acatgcaggc ctaccgagca tccggtctgt tgcccgacat ggcgaacaat aacgcggtac
     3361 atgttaacga aaaaatcata ccgaatgtgg ccgccggtct gataaaagtt aaacccctgg
     3421 cagagcgttt caccggagaa ggcgctatta agttcgtcga cgcatctcag gaaaaatatg
     3481 atgtaatcat aacctgtaca ggctatgaaa ggggagcccg cagaattcgg aaaaaatcgt
     3541 acgctaaggt tttccgggca tccgtaaggg ccgaaacttc ccgtcttcca gtctgcggct
     3601 ctgccgccag acgtaatcgc cggttaggtt gatgtgctcc cagcccagcg gcgacaggaa
     3661 ttgcagcagc tcgccgtcca ccggcttgcc ggcctcgacc aacccctggg tggcgcgttc
     3721 caggtacacc gtgttccaca gcacgatagc cgccgtcacc aggttgaggc cgctggcccg
     3781 gtagcgctgc tgctcgaagc tccgatccct gatttcccca aggcggttga agaacaccgc
     3841 cctggccagc gagttgcgcg cctcaccttt gttcaggccg gcatgcacgc ggcggcgcag
     3901 ttcaacactt tgcagccagt ccaggatgaa cagcgtgcgc tcgatccggc ccagctcgcg
     3961 cagggccacg gccagtccgt tctggcgcgg gtagctgccg agcttgcgca gcatcagcga
     4021 ggcggtgacg gtgccctgct tgatcgagct ggccaggcgc aggatgtcgt cccagtgggc
     4081 acgcacgtgc ttga
//
LOCUS       DAFAVF010000243         4034 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFAVF010000243 DAFAVF010000000
VERSION     DAFAVF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.247"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..261)
                     /locus_tag="LGK42_004299"
     CDS             complement(<1..261)
                     /locus_tag="LGK42_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBK2660460.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FYDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFRLLNRREADEE"
     gene            complement(243..665)
                     /locus_tag="LGK42_004300"
     CDS             complement(243..665)
                     /locus_tag="LGK42_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBK2660461.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMSEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(681..1421)
                     /locus_tag="LGK42_004301"
     CDS             complement(681..1421)
                     /locus_tag="LGK42_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660462.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            complement(1429..1824)
                     /locus_tag="LGK42_004302"
     CDS             complement(1429..1824)
                     /locus_tag="LGK42_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660463.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(1821..2399)
                     /locus_tag="LGK42_004303"
     CDS             complement(1821..2399)
                     /locus_tag="LGK42_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660464.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERAWLKRATVKNPQARIRVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(2411..2764)
                     /locus_tag="LGK42_004304"
     CDS             complement(2411..2764)
                     /locus_tag="LGK42_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBK2660465.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(2776..3171)
                     /locus_tag="LGK42_004305"
     CDS             complement(2776..3171)
                     /locus_tag="LGK42_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBK2660466.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(3213..>4034)
                     /locus_tag="LGK42_004306"
     CDS             complement(3213..>4034)
                     /locus_tag="LGK42_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBK2660467.1"
                     /translation="STSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRRRRII
                     LQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSGGTEW
                     SKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGSNSEL
                     ETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGLRTYG
                     CIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQLA"
BASE COUNT          837 a         1255 c         1030 g          912 t
ORIGIN      
        1 ctcttcgtca gcctcgcgcc ggttcagcag acggaaatcc agcggatgca tatccggatc
       61 gctgaaaaac aggctgagca cggtgtacgt cagcccggaa aagtgcatat ccagcagaac
      121 atcataaaaa taatgggtac tgtaaaagcg gtgccagtcg gcatactccg tggatgacat
      181 cccggcaagc atggcacgcc agtcgggtcg ccccatctca cgcgccagtt tcagggcaaa
      241 actcagctca ccgtcgaaca ctttcccgca gaaacaggct ctgcgggccc ggcgtcctct
      301 gtctgttcag gggcattatt caccacaaac tcagacatac cggacagccg gtacaccacg
      361 ttttcagcat gagaaattgc ctctgtaggc caggtggtaa gcacttcctg ctcaatctgt
      421 ttaacggctt cattcatgga aggctgcttt gtcttctgcg gatggttatg ccacagggac
      481 atcgctacca caaaagcacc ggttctgatg gcgtcttcca cagtaaactt ccggttgctg
      541 tctgactccg cctgttctgc ctgccgtttc atcagggcga gatgctcaat acgctgcagg
      601 gctgacagtt cagaaagcgt gacggtcacg ccgttatgtt caaatgattc ggttttcagg
      661 aacatcgctg actctccgga ttaactgtcg gtgacggtaa tttctgcaac cgcagcaagt
      721 tcaccattac cggatacaac cggaatgttg accttacctg cagcaacgcc tttcacggtg
      781 atggtcatac cactgaccga cacggtggct tttgttttat ccgctgacac cgcacggaag
      841 ctcttgtcag ttgcgccctc cggctggaat gccacggtca gcgtggtgct ctgccctttc
      901 accaccgagg agctggcagg cgtcacggtc ataccggttg ccgccgtcac cgtactgcga
      961 tcttctgcca ttgacggacg gcccacattg gtgaccttca ccgtacgggt gatcacttcc
     1021 ttcgccgtca ccgccttacc gatactgctg acccagccac ggaacacatc gaccgtgccg
     1081 ttcgggaagc ggattttata ggcacgggta tcaccttcat taaaccacgc cagcagcgcc
     1141 tgctgcccct gctctccggg catccacgcc agcgtgaagc tggtatctcc ggcggatttc
     1201 tgcccctgcc cggtcgcggt ccagtccgca tcttcatcat cgagataact gtcgtcatag
     1261 gactcagcgg tcagttcgcc gggcgtcagg tctttaactt ttgccagacg cgaccagtca
     1321 acgtctgaaa gcgggttcgc ataagggtcg ccgctcccgt tataaaccca cagggtggtt
     1381 ccggcacctt tcaccggtat tgctggattt ggtacaggca tatcgtcctc acatttcata
     1441 ggtaatgaca tacgtcagat cggcagaact ccacaggccc gcatcatcgt cgcgccggta
     1501 gtcatagcca ctggccacca tactggtgat caaatctgac agtgccggga tatcgctcat
     1561 caccggataa atccgggact ccatccacgc atccagctct gaatccggca cctgagcagg
     1621 caggaaaact tcgatatgca gctccgcctg ccaggtatca ctgtccagct cttcgcccgt
     1681 gtattcagcg ccggtgagat aaacggcaat tgccggaaaa tccgcctcat caaaaacagc
     1741 ggggcgacca tcaaaaagcg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc
     1801 tgcacggagt tcagtatgtt tcatcgcttt attaccattc tcagttgatg ctgcagcgca
     1861 tagcccagct ctttcggaag acgctcacgc cgtatccgct caatattctg tttaaacgcc
     1921 gtggtcagcg gcaccgccat cgggattttc accacatcaa tggggtaacg gtttttcccg
     1981 gccacacgct gcatgacatg ccagcggcca tttttcagtt gctgaataaa cgcgccggga
     2041 atacgacggt ttcccaccac aagcacgctg ccgccacctt tcagggatga acgctgcccc
     2101 tttttacgac gcctgcgtcg ggacaggaca atccgcgcgt tacccagctt tattacgggc
     2161 aaatcccccc ggttaaccct gattctggcc tgcggatttt tgaccgtggc ccttttcagc
     2221 caggcccttt cctttaccag tttccggcgt acctttgtct cacgggcaac ctgtgacgcc
     2281 gactgcgata tcgcggatga agcaacgcgg ttaatggcca ttgcggcggc accgggcacc
     2341 gccgttctgc tgatacggct gaggttttca acggcctgct caagaccttt tatggccata
     2401 catccccctt tcagcggcga cggttaacgg caggcggtac gccccgccca agccagagat
     2461 gacagcttcc gccatcatcc ggcgaaatcc ggtctatcca gaagttttcc tcaccgatgg
     2521 tcagcgtgtc gccgcgccgc agctgccgca catcatcagt ccggacaaac agggacgggc
     2581 tggagccttc aacgcgcacg ccctgtccgg catagctgat attttcaggg tcatcaaaaa
     2641 caccacgtat cacagcaccg gactgctcac cggatgtcat ggtggctgac gttcccatgt
     2701 acccgcgtat cgtttcatcg gcgcaggcaa tggcagcatc gaacaggtta tcgaaatcag
     2761 ccacagcgcc tcccgttatt gcattctggc caggccgcgc tctgtcattt cagctgccac
     2821 accggcagag acacggaacg ccgttcccgg cagcacaaat gccacagcct catcccgcgt
     2881 ggcgtgaagt gcatcagtat gcagcgtcac cagtgccaca accgtgacca gatcagccgt
     2941 atcaatcacg gtatccggct gcgctgatac aacctcattt tcatgtccgg tcagcgcatt
     3001 ttccgggctg acagatgtgt cctgaccggc agcgtcatcc gtgtcatcaa gctcctcttc
     3061 cagctctgcc acacggagtg ccagttcttc tttcgtcccc gtcaggctga catcacggtt
     3121 cagttgctca cccagcacct gaagacgggc aatcagttca tctttcgtca tggactcctc
     3181 cacagagaga aaatggcccc gaagggccat gattacgcca gttgtacgga cacgaacgca
     3241 tcagggtcag ccagcagcat cagcggtgct gactgaatca tggtgaactc acgcgccgga
     3301 tcgccggtgg tcacccagtt tttcgggtag cgggcagagg cgttaatacc ttcgcgctgt
     3361 gcgtccgcat cctgaatgca gccataggtg cgcagaccgc gtgcctgagt gttccccagc
     3421 accatcgtgt tgtccggaag gaagttcttt ttgacgtcgt tttccacgta ctgtccggaa
     3481 tacacgacga tcgccgtatc gccatacatc cccttatagg acaccgcttc gcccaggtct
     3541 tttaccgctg tctccagctc ggaattagag ccgcgacggg tatccagctt ctccttgacg
     3601 gctttgaagg aacggaacag cgcccagcct ttcggatcga acacgatgat attcaccaca
     3661 ccgctggcgt tcagcgcgta ggcttcgata tcgtcggtcg ggtcatacgt ggacttgtca
     3721 cgcttgctcc actccgtacc gccggactgc gtgatgttgt tcgccacact gcggcccata
     3781 tccacttcaa ccggatcgaa ggcttcaccg gtcatggtgt atttgccctt aagcacggca
     3841 gaaactgcct gcatctcttc gacctgagca atggccagct cttcgtctcg catgttctgc
     3901 aggatgatgc gacggcggcg gtaagccggg tccgccagat tctgtggatc ttcatccggc
     3961 aggcgacgca gggtcatctg cggattcacc tcatgcttgg gcttgacata tcccggtgta
     4021 aattcagagg tgga
//
LOCUS       DAFAVF010000244         4033 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFAVF010000244 DAFAVF010000000
VERSION     DAFAVF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.248"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            88..723
                     /locus_tag="LGK42_004307"
     CDS             88..723
                     /locus_tag="LGK42_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBK2660468.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPSPSVSADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            720..1067
                     /locus_tag="LGK42_004308"
     CDS             720..1067
                     /locus_tag="LGK42_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBK2660469.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            1072..1980
                     /locus_tag="LGK42_004309"
     CDS             1072..1980
                     /locus_tag="LGK42_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBK2660470.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITLADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            1973..2503
                     /locus_tag="LGK42_004310"
     CDS             1973..2503
                     /locus_tag="LGK42_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBK2660471.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            2514..>4033
                     /locus_tag="LGK42_004311"
     CDS             2514..>4033
                     /locus_tag="LGK42_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBK2660472.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PEAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVTSVALTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKVAYDLANGKYTAQDATTARKGLVQLSSATNSDSETLAATPKAVKVAYD
                     LANGKYTAQNATTARKGLVQLSSATNSDSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKRLFLRNIGATNSTTMSFSGGRGWFRLATVTMPQASTVVYISLIGGAGYNVNSPM
                     QAGISELVLRAGNGNPKGLTGALWRRTSVGFTNFAWVNTSGDTYDVYVEIGNYATGV"
BASE COUNT          962 a         1095 c         1129 g          847 t
ORIGIN      
        1 ccattacgct ctccttgaat gttgtctggt agttctacaa atgaatccag atagcataac
       61 ttttatatat tgtgcaatct cacatgcatg aacactctcg caaatattca ggaactcgcg
      121 cgcgcactgc gcaacatgat tcgcaccggc attatcgtcg aaaccgacct taacgccggt
      181 cgctgccgcg tgcagaccgg cggcatgtgc accgactggc ttcagtggct gacccatcgc
      241 gcaggacgtt cgcgcacatg gtgggcacct tccgtggggg aacaggtgct gattctggcc
      301 gtgggtggtg aactcgacac ggcgttcgtt ctgccgggga tttattccgg cgataacccc
      361 tcgccgtctg tgtcggcgga tgccctgcat atccgtttcc ctgacggggc ggtgattgaa
      421 tatgaacccg aaaccagtgc actcacggta agcggaatta aaacggccag cgtgacggct
      481 tctgattctg ttactgccac ggtgccggtg gtcatggtga aagcatcaac ccgcgtcacc
      541 ctggacaccc cggaggtggt ctgcaccaac aggctgatta ccggcacgct ggaagtgcag
      601 aagggcggga cgatgcgcgg caacattgaa cacaccggcg gtgaactctc atcaaacggt
      661 aaggtactgc atacccataa acaccccggc gacagcggcg gcacaaccgg gagtccttta
      721 tgacagcgcg ttatctcgga atgaatcgca gtgatggcct gactgtcact gaccttgagc
      781 atatcagcca gagtatcggc gatatcctgc gcacaccggt cggctcacgg gtgatgcgtc
      841 gtgattacgg ctcgttgctg gcgtcaatga ttgaccagcc gcagaccccg gcgcttgagt
      901 tgcagattaa ggtcgcctgt tacatggcgg tgctgaaatg ggaaccccgc gtcaccctgt
      961 catccgtcac cacggcgcgc agctttgatg ggcgaatgac ggtcacgtta accggccagc
     1021 acaacgacac cggccagcca ctttcgttaa ccatccctgt gagttgaaac catgccgatt
     1081 atcgacctga accagctacc cgcaccggat gtggtcgagg agctggactt tgaaaccatt
     1141 ctcgccgaac gcaaggcgac actgatttcc ctttacccgg aagaccagca ggaggcggtc
     1201 gcccgtaccc tgacactgga atctgagcct ctcgtcaaac tgctggaaga aaatgcttat
     1261 cgtgagctta tctggcgtca gcgtgtgaat gaggccgcac gggcggtgat gctggcctgt
     1321 gccgccggta atgaccttga tgtgattggt gccaattaca acaccacgcg cctgactatc
     1381 accctggcag atgattcgac cattccgccg acaccggcag tgatggaatc tgacaccgat
     1441 tatcgtctgc gtattcagca ggcttttgag ggcttaagcg tcgccgggtc agtgggagcc
     1501 tatcagtatc atggtcgcag tgctgacggg cgtgtcgcgg atatttctgt caccagtccg
     1561 tctcctgcct gtgtcaccat ctctgtgctg tcacgtgaaa ataacggcgt cgcatccgaa
     1621 gacctgctgg ctgtggtgcg taacgccctt aatggcgagg acgtcaggcc ggtggccgac
     1681 cgcgtgaccg tacagtctgc cgccatcgtt gaataccaga taaacgccac gctttacctt
     1741 taccctggtc ccgaaagcga acccatccgc gctgccgctg tgaaaaagct ggaagcgtat
     1801 atcacggcac agcaccggct ggggcgcgac atccgtctgt ctgccattta tgccgctttg
     1861 catgtggaag gtgtgcagcg tgtcgaactg gctgcaccac tggccgacat cgtgctcaac
     1921 agtacgcagg cgtctttctg taccgaatac cgcgtcgtga ccggaggctc ggatgagtga
     1981 ttcgcgactg ctgccgaccg gctcatcacc gcttgaggtc gccgccgcaa aagcctgtgc
     2041 ggaaattgaa aaaacgccgg tcagtattcg tgagctgtgg aacccggaca cctgtccggc
     2101 aaatctgctt ccgtggctgg cgtgggcgtt ttcggtcgac aggtgggatg aaaagtggcc
     2161 ggaagcgaca aaacgcgccg ttatccgcga tgcgtatttc attcactgtc ataaaggcac
     2221 aataggtgca atccggcgtg tggtggagcc gctcggctat ctcatcaatg tgacggagtg
     2281 gtgggaaaac agtgacccgc ccggcacctt ccggcttgat attggtgtac tggaaagcgg
     2341 catcacagag gcaatgtatc aggaaatgga acggctgatt gctgatgcca aacctgcaag
     2401 ccgccacctt attggcctga acattacccg ggacattccc ggctacctgt tcgccggtgg
     2461 tgtggcttac gacggcgatg taattacggt ttaccccgga taagtgagga ataatgagca
     2521 caaaattcag aaccgttatc accactgccg gtgcagcaaa gctggcagcg gcaaccgcac
     2581 cgggagggcg gaaggtcaac attaccacga tggccgtcgg ggatggcggt ggtaaattgc
     2641 ctgtcccgga agccggacag accgggctta tccatgaagt ctggcgacat gcgctgaaca
     2701 aaatcagcca ggacaaacga aacagtaatt atattatcgc agagctggtt attccgccgg
     2761 aggtgggcgg tttctggatg cgtgagcttg gcctgtacga tgatgcggga acgttaattg
     2821 ccgtggcgaa catggccgaa agttataagc cagctcttgc cgaaggctca gggcgttcgc
     2881 agacctgccg tatggtcatc atcgtcagca gtgtgacctc agtggcgctg accattgaca
     2941 ccacaacggt gatggcgacg caggattacg ttgatgacaa aattgcagaa catgaacagt
     3001 cacgacgtca cccggacgcc tcgctgaccg caaaaggttt tactcagtta agcagtgcga
     3061 ccaacagcac gtctgaaaca ctggctgcaa cgccaaaggc ggtaaaggcc gcatatgacc
     3121 ttgctaacgg gaaatatacc gcacaggacg ccaccaccgc gcgaaaaggt cttgtccagc
     3181 tcagtagtgc caccaacagc acgtctgaaa cgctcgccgc aacaccaaaa gcggtaaaag
     3241 cagcatatga ccttgctaac gggaaatata ccgcacagga tgccaccaca gcgcgaaaag
     3301 gccttgtcca gctcagtagc gccacaaaca gcgattctga aacgcttgcg gcaacgccaa
     3361 aggcggtaaa ggtcgcgtat gaccttgcta acgggaaata cactgcgcag gatgccacca
     3421 ctgcgcgaaa aggtcttgtc cagctcagta gcgccacaaa cagcgattct gaaacgcttg
     3481 cggcaacgcc taaggcggta aaggtcgcgt atgaccttgc taacgggaaa tacactgcac
     3541 agaatgccac cacagcgcga aaagggcttg ttcagctcag tagcgccacc aacagtgatt
     3601 cggaaacgct ggccgcaaca ccaaaagcgg tgaagtctgc ctatgacaat gctgaaaaac
     3661 gtcttcagaa agatcaaaat ggcgcggata ttcctgataa aagattattc ctgcgcaata
     3721 ttggagcaac aaattcaaca accatgtctt ttagtggtgg tagaggatgg ttcaggctgg
     3781 caactgtaac catgccacag gccagtaccg tggtttacat aagtctgatt ggtggtgccg
     3841 gatataatgt taactcccct atgcaggctg gcatatctga acttgttctt cgtgcgggaa
     3901 atggaaatcc aaaaggtctt actggtgcgt tatggcgacg gacctcggtt ggatttacta
     3961 attttgcatg ggtgaataca tccggtgata cctatgatgt ttatgttgaa ataggtaatt
     4021 acgccacagg tgt
//
LOCUS       DAFAVF010000245         4000 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFAVF010000245 DAFAVF010000000
VERSION     DAFAVF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.249"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1205
                     /gene="frc"
                     /locus_tag="LGK42_004312"
     CDS             <1..1205
                     /gene="frc"
                     /locus_tag="LGK42_004312"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBK2660473.1"
                     /translation="QSGPSCTQMLAWFGADVIKIERPGVGDVTRHQLRDIPDIDALYF
                     TMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFTWEHIQEINPR
                     LIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAAALGDSNTGMHL
                     LIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYLEEYPQYPNGT
                     FGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTCKAIGKPEWITD
                     PAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLSMKEISLDPSL
                     RQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQELGYSDDEIA
                     AMKQNHAI"
     gene            1259..2953
                     /gene="oxc"
                     /locus_tag="LGK42_004313"
     CDS             1259..2953
                     /gene="oxc"
                     /locus_tag="LGK42_004313"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBK2660474.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3023..3967
                     /gene="yfdV"
                     /locus_tag="LGK42_004314"
     CDS             3023..3967
                     /gene="yfdV"
                     /locus_tag="LGK42_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBK2660475.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
BASE COUNT         1034 a          862 c          977 g         1127 t
ORIGIN      
        1 tgcaatctgg cccatcttgt actcaaatgc tggcctggtt tggcgctgac gttattaaaa
       61 ttgaacgtcc cggcgttggt gacgtaacgc gtcaccagct gcgagatatt cctgatatcg
      121 atgcgcttta cttcaccatg cttaacagta acaaacgttc tattgagtta aataccaaaa
      181 cagcggaagg caaagaggta atggaaaagc tgatccgcga agctgatatc ttagtcgaga
      241 actttcatcc aggggccatt gatcacatgg gcttcacctg ggagcatatt caagaaatca
      301 atccacgtct gatttttggt tcgatcaaag ggtttgatga gtgttcgcct tatgtgaatg
      361 taaaagccta tgaaaacgtt gctcaggcag cgggtggcgc ggcatccact acgggttttt
      421 gggatggtcc gccgctggta agcgctgcag cgttgggtga cagcaacacc ggaatgcatt
      481 tgctgatcgg tttacttgct gctttgctgc atcgcgaaaa aacggggcgt gggcaacgag
      541 tcaccatgtc aatgcaggat gccgtattga acctttgccg cgtgaaatta cgtgaccagc
      601 agcgtctcga taaattgggt tatctggaag aatacccgca gtatccgaat ggtacatttg
      661 gtgatgcagt tccccgcggt ggtaatgcag gtggtggcgg tcagcctggc tggatcctga
      721 aatgtaaagg ctgggaaacc gatcctaacg cctatattta tttcactatt caggagcaaa
      781 actgggaaaa cacctgtaaa gccatcggca aaccagaatg gattaccgat ccggcataca
      841 gtacagccca tgcacgacag ccacatattt tcgatatttt tgctgaaatc gaaaaataca
      901 ctgtcactat tgataaacat gaagcggtgg cctatttgac tcagtttgat attccttgtg
      961 caccggtttt aagtatgaaa gaaatttcac ttgatccctc tttgcgccaa agtggcagtg
     1021 ttgttgaagt ggaacaaccg ttgcgtggaa aatatctgac cgttggttgt ccaatgaaat
     1081 tctctgcctt tacgccggat attaaagctg cgccgctatt aggtgaacat accgctgctg
     1141 tattgcagga gctaggttat agcgacgatg aaattgctgc aatgaagcaa aaccacgcca
     1201 tctgatatta acgggggctt ctggctccca atttataaaa atttcgaggt tattaatcat
     1261 gtcagatcaa cttcaaatga cagatggtat gcatatcatc gttgaagcat taaaacagaa
     1321 taatattgac actatttatg gtgttgtagg tattcctgtg acggatatgg cacgccatgc
     1381 ccaggcggaa ggcattcgtt atattggttt tcgtcatgag cagtcggcag gctatgccgc
     1441 tgcggcaagc ggttttctta cccaaaaacc ggggatctgc ctgacagttt ctgcgccagg
     1501 attcctcaat ggtttgaccg cattggccaa cgcaacggta aatggttttc cgatgatcat
     1561 gattagcggc tccagcgacc gcgcgatcgt cgacctacag caaggtgatt atgaagagct
     1621 ggaccaaatg aatgcggcaa aaccgtatgc caaagcagca tttcgcgtta atcagccgca
     1681 ggatcttggc attgcattgg cacgcgctat ccgggtctct gtatcgggtc gccctggcgg
     1741 agtttatctt gatttgccag caaatgtcct ggccgcgacg atggaaaaag acgaagcgtt
     1801 aaccacgatt gttaaagttg aaaatccatc gccagcatta ttgccatgcc cgaagtcagt
     1861 cactagcgca atttcgcttt tagcaaaagc tgaacggcca ttaattatcc ttggcaaagg
     1921 cgcggcgtat tcacaagctg atgaacagct tcgtgaattt attgaaagta ctcagattcc
     1981 attcctgcca atgtctatgg cgaaagggat ccttgaagat acgcatccac tttctgcggc
     2041 agctgcgcgt tcgtttgccc tggcaaatgc tgacgttgtc atgcttgttg gtgcacgact
     2101 gaattggtta ttggcacacg gtaaaaaagg atgggcggca gatacacagt ttattcaact
     2161 ggatattgaa ccgcaggaaa ttgacagcaa ccgccccatt gctgtgccag tcgttggtga
     2221 tattgcatcc agtatgcaag gtatgctggc agaactgaaa caaaacacat ttacgactcc
     2281 actggtatgg cgcgatattt taaatatcca caagcagcaa aatgcacaaa aaatgcatga
     2341 aaaattaagt acagatactc aaccattaaa ttactttaat gcattaagtg ctgtgcgcga
     2401 cgtattgcgc gagaaccagg atatttattt agttaatgaa ggtgcaaata ccctggataa
     2461 tgcacgaaat attattgata tgtataaacc acgtcgtcgt ctggattgtg gtacctgggg
     2521 tgtcatgggc atcggtatgg gctatgccat cggtgctagc gtgacttctg gttctccggt
     2581 tgtcgccatt gaaggtgata gtgcttttgg tttcagtggg atggaaattg aaacgatttg
     2641 tcgatataac ctgccggtga cgatcgttat ttttaataat ggcggcatct acagaggaga
     2701 cggtgttgat ctcagtggcg ctggtgcacc atcaccaacg gatctgttgc accatgcaag
     2761 gtatgacaaa ttaatggatg cgtttcgtgg cgttggctat aacgtcacca cgacagatga
     2821 acttcgtcat gctttaacca ccggtattca gtcgcgcaaa ccgaccatta ttaatgtggt
     2881 catcgaccct gcagcaggaa ctgaaagtgg ccatattacc aaacttaacc caaaacaagt
     2941 cgctggtaat taaatactga catcgtctcc tcttgccaac tcattatgcg atggtgaggg
     3001 gacccttatc ggaaatacta ttatgctaac attttttatt ggcgatttat tgcctattat
     3061 cgtaatcatg ctgttgggtt attttagcgg cagacgagaa acattttcag aagatcaagc
     3121 tcgggcattt aataaactgg tattaaacta cgcgcttcct gcggctctat ttgtatctat
     3181 tactcgggca aacagggaaa tgatttttgc ggacactcgt ctgacccttg tatcacttgt
     3241 ggttattgtc ggatgtttct ttttctcctg gttcggttgc tacaaatttt ttaaacgtac
     3301 ccatgcagaa gcagctgtat gtgcattaat tgcaggttca cctaccattg gattcctggg
     3361 gtttgcagtt ctcgatccta tttatggtga ttccgtatca acaggtttag tggtagcaat
     3421 tatttctatt attgttaacg caattactat tccaattggt ctgtatttgc tgaatccttc
     3481 ctcaggagcg gatggtaaga agaatagtaa tctgagcgca ttaatttctg cggcaaagga
     3541 gccagttgta tgggcacctg ttctggcaac gatcctggtg ttggttgggg taaaaattcc
     3601 ggcagcatgg gacccaacct ttaatctgat tgcgaaggct aactcagggg tagcggtatt
     3661 cgctgcgggg ttgactctgg ctgcacataa attcgagttc agtgccgaaa ttgcttataa
     3721 caccttcctg aagctgattc tgatgccact ggcgctgctt ctcgttggta tggcatgtca
     3781 tttgaacagc gaacatttgc agatgatggt actggcaggc gcattaccgc cggcattctc
     3841 cggaatcatt attgccagcc ggtttaatgt ctacacccgc actggtacag cgtcattggc
     3901 tgtgagcgta ctgggttttg tcgtcacggc tcccttgtgg atttatgtca gtcgactggt
     3961 ttcataatta atcatgttat tgattttaca taaatgccga
//
LOCUS       DAFAVF010000246         3994 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFAVF010000246 DAFAVF010000000
VERSION     DAFAVF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.250"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..221
                     /locus_tag="LGK42_004315"
     CDS             <1..221
                     /locus_tag="LGK42_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HBK2660476.1"
                     /translation="NAASHSKNLIDIIDSGCELEDISSIIPENLSGNIDKLINQTLDF
                     IRGAPDFGGAVDKEIILVKLKYIVNICW"
     gene            275..>3994
                     /locus_tag="LGK42_004316"
     CDS             275..>3994
                     /locus_tag="LGK42_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2660477.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPKKKD
                     SPNYGNPVNPVLGAKVLPGETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEVSYSRSESFWLARGGVAEQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPPPPAYRVLTGVVDG
                     FGRTLTFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAGP
                     RSASSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLA
                     AETSRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGKR
                     STIAYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTT
                     LSHTCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPE
                     GVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQL
                     EAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRG"
BASE COUNT          876 a         1055 c         1399 g          664 t
ORIGIN      
        1 tgaatgctgc atcgcactct aaaaacttaa ttgacattat agattctggt tgtgagcttg
       61 aagatatatc tagcatcatt cctgaaaatt tatcaggtaa tattgataaa ttaattaacc
      121 agacgctgga ttttattcga ggtgctcctg attttggcgg tgcagtcgat aaggaaatta
      181 ttttagtcaa attaaaatat atcgttaata tatgttggtg atgttttgtt gagggctttt
      241 ttattgttag tggtataaat ataagggagt gagaatgagc ggaaaaccgg cggcgcggca
      301 gggcgacatg acccgcaagg gactggatat tgtgcagggt tcagcagggg tgctgatagg
      361 cgcgccgacg ggggttgcct gctcggtgtg tcctaaaaaa aaggacagtc caaattatgg
      421 caacccggtg aacccggtgc tgggtgcaaa ggtgctgccg ggcgagacgg acattgcgct
      481 gcccggcccg ctgccgttta ttctttcccg cgcctacagc agctaccgga ccagaacacc
      541 cgcgccggtg ggggtgtttg gtcccggctg gaaagcgccc ttcgatatcc gcttacagat
      601 acgcgatgaa ggcctgatac tcaacgacag cggcggcagg agcattcact ttgagccgct
      661 gtttcccggc gaggtaagct acagccgcag cgagtcgttc tggctggcgc ggggcggggt
      721 ggcggagcag cacagttcgc agccgctaag cgcgctctgg caggtgctgc cggaagatgt
      781 tcgcctgagt ccgcatgtgt acctggcgac aaacagcctt caggggccgt ggtggatact
      841 cagctggccg gagcgggtgc cgggggcgga cgaggtgctg ccgcctccgc cgcccgcata
      901 ccgggtgctg acgggggtgg tggacggctt cgggcggacg ctgactttcc accgggcagc
      961 taagggtgac gtggcgggcg cggtgacggg ggtgacggac ggcgcgggcc gccgttttca
     1021 cctggcgctg accacgcagg cgcagcgggc ggaagccttc cgtaaacagc gcgccacgtc
     1081 tttatcttcc cctgccggtc cccgttctgc ttcctcttct tctgctttcc ccgacacgct
     1141 gcccgccggt acagaatacg gtgccgataa cggtatccgg ctggaggcgg tgtggctgac
     1201 acacgacccg gcatacccgg atgaacagcc gactgcgccg ctggcgcgct acacgtacac
     1261 ggccagcgga gaactgcggg cggtgtatga ccgcagcggg acgcaggttc gcgggtttac
     1321 ttatgatgcg gagcacgccg ggcggatggt ggcacaccat tatgcgggga ggccggagag
     1381 ccgctaccgg tatgatgata ccgggcgggt gacggagcag gtcaacccgg aggggctgga
     1441 ctatcgcttt gagtacgggc aggaccgtgt gaccatcacg gacagcctga accggcggga
     1501 ggtgctgtac acggagggcg aaggcggcct gaagcgtgtg gtgaagaagg agcatgcgga
     1561 cggcagcatc acccgcagcg agtatgatga ggcggggagg ctgaaggcgc agacggatgc
     1621 ggcgggacgg cggacggagt accgactgca tatggcgtct ggaaagctga cttcagtgat
     1681 actgccggat ggcaggacgg tacggtatgg ctataacagc cagcggcagg tgacgtcggt
     1741 gacgtacccg gacgggcttc gcagcagccg ggagtatgat gagaagggaa ggctggcggc
     1801 ggagacctca cgcagcggtg agacgacgag ttacagctat gatgacccgg cgagtgagct
     1861 gccgacaggg atacaggacg cgacgggcag tacaaaacag atggcatgga gccgttacgg
     1921 tcaactgctg gcctttacgg actgctcggg gtacacgacg cggtatgagt acgaccggta
     1981 cggtcaacaa atcgccgttc accgggaaga aggcatcagc acttacagca gttataaccc
     2041 gcgtgggcaa atggtcagtc agaaggatgc gcaggggcgt gaaacccgtt atgagtacag
     2101 cgccgcagga gacctgacag caacggtatc accggacggt aaacgcagca ccatcgcata
     2161 tgataaacgc gggcgtccgg tgagtgtgac ggaaggtgga ctgacgcgca gcatggggta
     2221 tgacgctgcc gggcgcatca ccgtgctgac caacgagaac ggcagccagt ccacgttccg
     2281 gtatgacccg gtggacaggc tgactgaaca gcgcggtttt gacggccgga cgcaacgtta
     2341 ccactatgac ctgaccggaa aactcacgca gagtgaagac gaggggctta tcaccctctg
     2401 gcactacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc cggcagagca
     2461 gtggcagtat gatgagcacg ggtggttaac caccctcagc catacctgtg aaggccaccg
     2521 cgtgtcggtc cactacggct atgacgataa aggccgcctg acgggtgaac ggcagacggt
     2581 ggagaacccg gagacagggg agatgctgtg ggagcatgag acggggcatg cgtacagcga
     2641 acaggggctg gcgacccgtc aggagccgga cggtctgccg ccggtggagt ggctgacgta
     2701 tggcagcggt tatcttgcgg ggatgaagct gggcggaacg ccgctggtcg agtacacgcg
     2761 ggaccggctg caccgcgaga cggcccgtag cttcggcggg gcgggcagta cagcggggta
     2821 cgaacaggcc acggcgtaca ccctcacagg gcagctacag agcaggcacc tgaaccttcc
     2881 gcagcttgac tgtgactata cgtggaacga taacggacaa ctggtgcgca tcagcggccc
     2941 gcaggagtgc cgggagtacc gttacagcgg cacgggaagg ctgacgggcg tccacactac
     3001 ggcagcgaac ctggatatcg atatcccgta tgcgacggac ccggcaggaa accggctgcc
     3061 ggacccggaa ctgcacccgg acagcaccct cacggcgtgg ccggataacc gcatcgcgga
     3121 agatgcgcac tatgtctacc gctacgatga atacggcagg ctggcggaga agacggaccg
     3181 catcccggaa ggggtgatac ggatgcacga cgagcggacc caccactacc actacgacag
     3241 ccagcaccgt ctggtgttct acacgcggat acagcacggt gagccgcagg tggagagccg
     3301 ctacctctat gacccgctgg gccgccggac gggaaaacgg gtgtggcggc gggagcgtga
     3361 cctgacgggg tggatgtcgc tgtcgcgtaa accggaggag acctggtacg ggtgggacgg
     3421 tgacaggctg accactgtac agacccaaca gacaagaatc cagacggtat accagccggg
     3481 aagcttcacg ccgctcctga gaatcgaaac agagaatggt gaacaggcga aggcgcggca
     3541 ccgtagcctg gcggaggtgt tgcaggagga cacgggtgtg acgctaccgg cggagctggc
     3601 ggtgatgctg gggaggctgg agcgggaact gcggcagggc agcgtgagtg aagaaagcca
     3661 gcagtggctt gcgcagtgcg ggctgacggc ggaacagatg gccgcgcaac tggaggcgga
     3721 atacatcccg gagaggaaac ttcatcttta ccactgtgac caccgtgggc tgccgctggc
     3781 gcttatcagc ccggaagggg aaacggcgtg gcagggggag tatgacgagt ggggaaacct
     3841 gctgggcgag gaaagcgcgc agcaccttca gcagtcactc cgtctgccgg gacagcagta
     3901 tgatgaggag tcggggctgt actacaaccg caaccggtac tatgatccgt tgcaggggag
     3961 atatatcacc caggacccga tagggctgag aggt
//
LOCUS       DAFAVF010000247         3984 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFAVF010000247 DAFAVF010000000
VERSION     DAFAVF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.251"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            394..1425
                     /locus_tag="LGK42_004317"
     CDS             394..1425
                     /locus_tag="LGK42_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK2660478.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSDETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            2121..2333
                     /locus_tag="LGK42_004318"
     CDS             2121..2333
                     /locus_tag="LGK42_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660479.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISWTYRKLKNQTIPLSTPYAT"
     gene            complement(2448..3656)
                     /locus_tag="LGK42_004319"
     CDS             complement(2448..3656)
                     /locus_tag="LGK42_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBK2660480.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(3637..3909)
                     /locus_tag="LGK42_004320"
     CDS             complement(3637..3909)
                     /locus_tag="LGK42_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660481.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          957 a         1138 c         1019 g          870 t
ORIGIN      
        1 ttaacttatt agaggtatca gtgaggaggc actggaagag aagagatcgt tgtaatgctt
       61 ttcaaattaa cgtaaagcgg gtatatttcg gctgttatta gctgtgcaga gggtggcact
      121 ctgtggagca aagcggcgaa agccggacgg cagaatgcgc cataaggcat tcaggagaga
      181 tggcatgtac ggacagtaag tcagaagact gaagatgttc cggaagccat aaaaggaaaa
      241 cccccactat ctttcttacg aacttggcgg aacgacgaaa gatagtgggg gcctcacaga
      301 atacgggtaa agtataatga aaccgtacca gagattcaac cctgtgcagt gtataaatac
      361 acggcacaat cgctccgcca taagcgacag cttgtggcag gtctgaagaa tactccatat
      421 aacgcagtac actggagtca gttagcaccc gaagagcaga tccgtttctg ggaagactat
      481 gaagcgggaa gggcgaccac tttcctggtt gaaccggaaa ggaagcgcac gaagcgtcgt
      541 cgtggtgagc actccaccaa acccaaatgc gaaaatccgt cctggtatcg tcctgagcgc
      601 tataaggcgc tgagcgggca gctcgggcac gcctacaacc gtctggtgaa aaaggacccg
      661 gtgaccggcg agcagagcct gcgcatgcac atgtctctgc atccttttta cgtgcagaaa
      721 cgaacgtatg ccggtcgcaa atatgctttc cgtccggaaa aacaacgcct cctcgatgcc
      781 gtctggccgg ttctggtcag cttcagtgat gcgggcacac ataccgtagg catgagtgtt
      841 tcccgtctgg ccagagaaat cagcccgaaa gacagcaagg ggaaggttat tccggaactg
      901 gaagtgacgg tctcccgcct ttcccgtttg ctggccgaac aggtacgttt tggtgtgctg
      961 ggtgtttcag atgaaaccct gtgggaccgt gaaacccgcc agcgtctgcc acgctacgtc
     1021 tggataacac cggcaggctg gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag
     1081 cagcagaaac gactgcgtga aagtgaaatc cgccagcagc tcattcggga aggtgttctg
     1141 cgtgaggatg aagatatctc cgtacatgcg gccagaaaac gctggtatct gcagcgcagt
     1201 caggatgcgc tgaaacaccg tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg
     1261 aagaaactgc ctgccgacca gcagattcat gagatggcag agtatctcag gaagcgtctg
     1321 cctccggatg aagcctattt ttgttccgat gaccatctga agcgaatggc catcagggag
     1381 ttgcgtcagc ttgaactgac gctggctgcc ccgccaccgc actaaacagc accattccct
     1441 cagcactgaa tcatcaccag cccctccggg gctttcggcg ctggttccgc tcagcccaaa
     1501 atccgcagta atcaccttaa atcccctcag aggggcatat ctgcccataa aaccacgcat
     1561 cagtcatcag aacatggcca cgttgtttca gttatccaca taaatccgca aacaaagaac
     1621 tttaagaagc tgcaaacctg aaacagcaaa cctgcaatat agtcttaacc ccattattta
     1681 atcccctgcg ttgcttcgcc gcagggaaaa tctttatctc tgagaccact gtgaacaaat
     1741 acaaagaggc cttcgcttgc agcggccaag gccgcgccgc tcagaatcta aaagcacctc
     1801 ccacgctgac gcgcgggccc cgaacctcac cgttctgaaa ccacaacaaa aaaacatcag
     1861 gaataaaaac accacacaaa cgcagcaccg tgcccacccc tcataactga aaagcgaggc
     1921 cgcccccgcc caaagggcgg gaacaacatc gcttttaatt atgaatgttg taactacatt
     1981 ttcatcgctg tcagtcttct ggctggacgt tctcagtaca cgctcgtaag cggccctgac
     2041 ggcccgctaa cgcggagata cgccccgact tcgggtaaac cctcgtcggg accactccga
     2101 ccgcgcacag aaactgtctc atggctgaaa gcgggtatgg cttagcaggg ctggggatgg
     2161 gtaaggtgaa atctatcaat cagtaccggc tgacgccggg cttcggcggt tttactccgg
     2221 tatcacatgt aaccacagcg tgccgccttc catgccgctg gcgcggcata tcctggacgt
     2281 ataggaaatt gaaaaaccag acgataccgc tgtccacgcc ttatgccacg tgagcctttc
     2341 tgcttttccc tccactccgg tggtttctct ctctgcatgg tcgattttta taccgtaaca
     2401 ggctgttttt tatacctgca ccgattcccg ggcacactta gcctgtgtca ggccggcata
     2461 tacctcagca gactgatatc ccgcgcgttt ttcttcagcc ctgatacatt cagccccgca
     2521 atggcccggc ggtataccat ccggccgcca cacagcacgc attcgaacgg gtcacgactc
     2581 aggaactgtt tcaccatttg tgcatagcac actttcgcca ctggttccgg cttatccatc
     2641 cccagagcac ggtacacctg cggcagcttc tctccacaca cccggttggc aaggaacccg
     2701 aagtatctca ccatcttaaa aaacttctcc gggatgtgct gtttcagcct cgcgaccagc
     2761 tcccgctgtg tcagcgtttc cgtcgccgtt tctcccgttt tgtggtccag gtaacggaag
     2821 ctcagactcg ctcctccgtt gtaatgcgcc agccgggaag ccgctattgg tggcttcttc
     2881 agataacgac ccaggtagcg tgccgtattc cgccctccgg ctgtcttctt cgacatgtac
     2941 acatgccagt attttccgcc ggctttcagc accaggcttc tccactgtga ttccgtcgtg
     3001 atatgtgaca acgattccgg catcgccaga ccttctgacc acgctttcag aagcagctgc
     3061 cgcatattcc acatccaccg cgaacgcatc gcgtctttca ggaagctcag ctttttccag
     3121 tgtccgtgtt tattcagacc tccgcaggtc actgacacat gcacatgcgg atgccagttg
     3181 agacgacggc catacgtatg gatggcgcag aagataccgg gttccagacc ccgtttccgg
     3241 gctgcataca gcagattctc caccgccaga cggcacacgt cattcagcag ccagcggttg
     3301 ctttcgaaca ccggccacag cgtgtccggc agggtgaaga ccaggtgcac ccagtcgcag
     3361 tcaggaagac gattcagctg tgtcgctatc cacaggtctg tggccttttt tccgcaggac
     3421 gggcaggcac ggctgccaca tgagttagtg acgtatctga cgtgggggca ctcaggttta
     3481 tcgcagttgt attccttcac acccagtatc cgcgtgccgc aggccagcat ttttgtgaca
     3541 gcctcaacct cgatatcgcg caggccgccc gcatccagga aggatgtcca gcactggttg
     3601 gctgtgaaca ggcgtttcag cggtcggggc tgaaaatcac caccacttcg ggtcatccgc
     3661 cttcatctcc gcttcagttt cgtataaatc aaaacgacgg caggtatggc agaacgtgac
     3721 gtattcgtac ggtgaatccg ggtacatttt gtggaaggtt tcccagaaac agaccgtaca
     3781 ggagggatga ccggcgataa agtctgtaaa tacagactga taagggtgat tattggccct
     3841 ggcgacggct ttcagaacct ctttcaccat tctggtgtgg actttctggt gctccaggtt
     3901 gtgtgacatg ggaactcatt ctggatggtt actctgaaag ctcatattct gccacaccac
     3961 gatttgcagc cgccaggctg ccgt
//
LOCUS       DAFAVF010000248         3951 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFAVF010000248 DAFAVF010000000
VERSION     DAFAVF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.252"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..812
                     /locus_tag="LGK42_004321"
     CDS             <1..812
                     /locus_tag="LGK42_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBK2660482.1"
                     /translation="AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHL
                     HEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRN
                     PPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRET
                     IDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSR
                     FYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1021..1986
                     /gene="lipA"
                     /locus_tag="LGK42_004322"
     CDS             1021..1986
                     /gene="lipA"
                     /locus_tag="LGK42_004322"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBK2660483.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2087..2290)
                     /gene="tatE"
                     /locus_tag="LGK42_004323"
     CDS             complement(2087..2290)
                     /gene="tatE"
                     /locus_tag="LGK42_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBK2660484.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2419..3207)
                     /locus_tag="LGK42_004324"
     CDS             complement(2419..3207)
                     /locus_tag="LGK42_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBK2660485.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3300..3683
                     /gene="crcB"
                     /locus_tag="LGK42_004325"
     CDS             3300..3683
                     /gene="crcB"
                     /locus_tag="LGK42_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBK2660486.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3738..3947)
                     /gene="cspE"
                     /locus_tag="LGK42_004326"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3738..3947)
                     /gene="cspE"
                     /locus_tag="LGK42_004326"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBK2660487.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1014 a         1029 c          917 g          991 t
ORIGIN      
        1 atgcagcgaa agtgcttaat ctgaccccct cggcaatcag tcagtctatt cagaaactgc
       61 gcgttatatt ccctgaccca ttgtttattc gcaaaggcca gggtgtcact cctaccgcat
      121 ttgcgatgca tctacatgag tatatcagtc agggccttga gtccattctt ggcgcgctgg
      181 atatcgaagg aagctatgat aagcaacgaa cgataactat tgcgaccact ccctcggtcg
      241 gagccctggt ccttcctgtc atctatcggg cgattaaaac tcactatccg cagcttttac
      301 tgcgcaaccc gcccatcagc gacgcggaaa accaactcag tcagtttcaa accgatctca
      361 tcatcgataa catgttttgc accaatcgta cggtgcaaca tcatgttctg ttcaccgaca
      421 atatggtgtt aatttgccgc gagggaaatc cactactctc tttagaagat gacagagaga
      481 ctatcgacaa cgctgcgcat gtacttctgt taccggaaga gcaaaatttc agcggtctgc
      541 ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa tttcaccagc tacaacattt
      601 tgacaatcgc tgcactggtt gccaacagtg acatgttagc gattattccc agccgttttt
      661 ataacctgtt tagccgctgc tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg
      721 agcaaataga tttctctatc cactacaaca aattcagcct gcgtgatccc atcctgcacg
      781 gagtaataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc agatcacagc
      841 gcagaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac cgctttggct
      901 gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata gttgataatt
      961 acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct
     1021 atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc
     1081 cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg
     1141 atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc
     1201 aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc
     1261 aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc
     1321 tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg
     1381 cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac
     1441 ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc
     1501 ccgcaaatca aaattgaaac tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg
     1561 gatattctga ctgcaacgcc accagatgtg ttcaaccata acctggaaaa cgtaccgcgt
     1621 atttaccgtc aggtacggcc tggtgcagat tacaactggt cgctgaagct gctggaacgc
     1681 tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg actgggtgaa
     1741 accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta
     1801 acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc
     1861 ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca
     1921 tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt
     1981 aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt ttgaagcggg
     2041 ttttttgtat cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag
     2101 agagcttttc agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat
     2161 tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact
     2221 tcttagtccc aaacagcaga acgaccagcg ccgcaactac tagcagtttg gtaatactaa
     2281 tctcacccat agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag
     2341 agaatattaa cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa
     2401 gcgccgagta aaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagac
     2461 gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc
     2521 aggcatttct gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgacc
     2581 gatatttttg ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg
     2641 agcggcaagc aacgttgacc agtgatgctc tttgagcggc ccgcgaaccc aggcggcagg
     2701 aagtaccagg atttcagctc cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa
     2761 gtcataacag gtcatcagac cgaccttcat cccctccacc tcgagtaacg gagcgatttc
     2821 attaccagca tcaacacggc gtgattcctg aatggcaaat gcatcataga gatgcaattt
     2881 ggcataacgg gcgacgatgt ttcctgcctg tagcgccacc agcatattcc atgcgcgccc
     2941 cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg
     3001 ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct gatttaaccg atagatctgc
     3061 atcatgatca tcgcgcgcca gcaatgcttc cggcaggacg aacagcgata cgtcgttttc
     3121 cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc ttttcccaca cagatgtaac
     3181 agcaaactgt cctgcggcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca
     3241 acgacgatta ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag
     3301 tgttacaact tcttttagca gtttttattg gcggtggtac gggaagcgtg gcgagatggc
     3361 tgttaagtat gcgatttaac ccgctgcatc aggcgattcc gttggggacg ctggcagcaa
     3421 acctgattgg ggcattcatc ataggaatgg gattcgcatg gttcagcagg atgacgaaca
     3481 ttgatccagt gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct
     3541 caacattttc ggcagaagtg gtgtttttgt tacaagaggg tcgctttggc tgggcattac
     3601 tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac agcactggct ttctggctgt
     3661 tttcggcctc aaccgcacac taaagaccaa aaaaaaaccc gctgattaag cgggttttga
     3721 attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc tttggcaccg
     3781 ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc attagtctgg
     3841 attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat
     3901 cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa a
//
LOCUS       DAFAVF010000249         3944 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFAVF010000249 DAFAVF010000000
VERSION     DAFAVF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.253"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            98..1756
                     /gene="dpiB"
                     /locus_tag="LGK42_004327"
     CDS             98..1756
                     /gene="dpiB"
                     /locus_tag="LGK42_004327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBK2660488.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVMRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1725..2405
                     /gene="dpiA"
                     /locus_tag="LGK42_004328"
                     /gene_synonym="citB"
     CDS             1725..2405
                     /gene="dpiA"
                     /locus_tag="LGK42_004328"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBK2660489.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2446..3831)
                     /gene="dcuC"
                     /locus_tag="LGK42_004329"
     CDS             complement(2446..3831)
                     /gene="dcuC"
                     /locus_tag="LGK42_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBK2660490.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1074 a          940 c         1014 g          916 t
ORIGIN      
        1 taaaatttgc cacgatcata attaatatct atgtattttg attcaacatt ttaattacat
       61 ccgtcaaaga ggctcgggac aacccgcaag gaaaacaatg ttgcagctta acgagaataa
      121 acagtttgca tttttccaaa gactggcatt tccgctgcgt atctttttgc tgattctggt
      181 gttctcaata tttgtcattg cagccctggc gcaatatttt acggccagtt ttgaggacta
      241 tttaacgctt catgtacgcg acatggcaat gaatcaggcg aaaattattg cctccaatga
      301 cagtgtcatc tctgcggtga aaacgcgtga ctacaaacgg ctggcgacca tcgctaacaa
      361 attacaaaga gataccgatt ttgattatgt ggtgattggc gaccggcact cgatccgcct
      421 ttaccatcct aatccggaga aaattggtta tcctatgcag ttcaccaaac agggcgcgct
      481 ggagaaaggg gagagctact tcattaccgg gaaagggtca atgggtatgg cgatgcgcgc
      541 caaaacgcca atctttgatg acgatggaaa agtcatcggc gtggtgtcga ttggctacct
      601 ggtgagtaaa atcgatagct ggcgggctga gtttttatta ccgatggcag gcgtgtttgt
      661 cgtgctgtta gggattctga tgttgctgtc gtggttcctg gccgcgcata tccgtcggca
      721 gatgatgggc atggagccaa agcaaatcgc acgggtggtc cgtcagcaag aggcgctgtt
      781 tagttcggtt tatgaagggc tgattgcggt ggatccgcac ggttacatta ccgccatcaa
      841 tcgtaacgca agaaagatgc tggggctgag ttcccctgga cggcaatggt tgggtaaacc
      901 cattgctgaa gtggtcaggc ccgccgattt ctttaccgaa cagattgatg aaaaacgtca
      961 ggatgtggtg gcgaacttta acggtctgag cgttattgcc aaccgggaag ctattcgttc
     1021 aggtgatgat ttgctggggg ccattatcag ctttcgtagt aaagacgaaa tttccaccct
     1081 caatgcgcaa ctgacgcaaa taaaacaata cgttgagagc cttcgtacat tgcgacacga
     1141 gcatctcaat tggatgtcga cgctcaatgg tctgttgcag atgaaagagt atgatcgcgt
     1201 gctggcgatg gtgcaggggg agtctcaggc ccagcaacag cttattgaca gcctgcgcga
     1261 ggcgtttgcc gatcgccagg tggcggggct gctttttggt aaagtgcagc gcgcccgcga
     1321 actggggcta aaaatgatca tcgtccccgg tagccagctt tcgcaactgc cgccaggact
     1381 ggatagcacc gagtttgccg ccattgttgg caatttactt gataacgcct tcgaagccag
     1441 cctgcgtagc gatgaaggaa acaagatcgt tgaattattc ctcagcgatg aaggcgatga
     1501 tgtggtgatt gaagtcgccg atcagggctg cggcgttcca gagtctctac gagacaaaat
     1561 atttgagcag ggggtcagta cgcgtgctga cgagcccggt gaacatggca ttgggttgta
     1621 cttgattgcc agctacgtaa tgcgctgcgg tggtgttatc actctcgaag ataatgatcc
     1681 ctgcggtacc ttattttcaa tctatattcc gaaagtgaaa cctaatgaca gctccattaa
     1741 ccctattgat cgttgaggac gaaacgccgc tggcagagat gcatgcggaa tatattcgtc
     1801 acattcccgg attcagtcag atattactgg cgggaaacct ggcgcaggcc cgaatgatga
     1861 tcgagcgttt taagccgggg ctaatcttgc tcgataacta tcttcctgac ggtagaggga
     1921 ttaatttact gcatgaactg gtgcaggcgc attatcccgg cgacgtggtg tttaccactg
     1981 cagccagcga tatggaaacg gtgtctgaag ccgtacgttg tggtgtattt gattatctca
     2041 ttaagcccat tgcctatgaa cggctggggc aaacgctaac ccgtttccgc cagcgtaaac
     2101 atatgctgga aagtattgat agcgccagcc agaagcaaat tgatgagatg tttaatgctt
     2161 atgctcgcgg tgaacctaag gacgagctac cgaccggcat tgaccctttg acgctaaacg
     2221 cggtgcgaaa actgttcaaa gagcctggtg tgcaacatac ggcagaaacg gtggcgcagg
     2281 cactgaccat cagccgcacc actgccaggc gttatcttga atattgcgcc agccgccatc
     2341 tgattattgc tgaaattgtt cacggcaaag ttggcagacc gcaacgtata taccacagtg
     2401 ggtgacatga aaaagccgga ggaaacttgc ctccggcatt actatttact tgcctgtgac
     2461 cgctgctgcc gttcctggca ccatcagctc tgtagcaacg ataacaatca ctagcccaac
     2521 aagcaccggc accgaggtgc gttttacgac ttcaaatggt gagattttcg ccatcccggc
     2581 aaccgcaacg actacgccag aaacgggtga tagggtacga cccaggtttg acgcctgcag
     2641 catcgggata gtcaaatacg ccgggttaat gccggaagag tgcgccagtt tcgggatcat
     2701 ctcaacaaac gcataaaacg gcgcattgcc tgaaccggtc gtgactgccg ccagcatggt
     2761 cagaatcacc aataccagca tcaggatgat actcgctgaa ccaaacgagg tagcgataga
     2821 aatcagactt tgaataaagc cgatggtgct aagcccctga gcgaataccc cagcggcaac
     2881 cagcagcatc accacgttag caaacgcatc tgccatcccg cgataagcca cttccagacc
     2941 agagaaaact ttctgggtat taaagctgcg gaggaactcc agaatggagg caatcagcat
     3001 acaaatcacc agaatagtga tgatgtgtaa ttgcggaccc catttaccgt caaaaatcag
     3061 tacaccgatg atcggcgtga acggcaaaat ggcataaaac gcaggagcag tggtggtgat
     3121 ttcactgaca tctaacattt catgagagat gtgctctttt ttatccagat aacgttgcca
     3181 gaagaagtgg gcgatcgcca tgccgataat tgcagcaatt gagataggca gcgtcgtttt
     3241 gaaggcgaag tcaatcagcg acatttcgga agcttgcgcc gccagcacca catcccctga
     3301 agtcggtgcg agaataatcg ccgccgggga ggcacaaatg gcagcagctg cgccacgact
     3361 gataccaacg tttaccatca ccggaaatag ggttgccatc agcaaaacac ccagaccggt
     3421 tgcggaagag acggccagag acatcagaca ggcgacaaaa taggcggcaa tcatcagcag
     3481 gtaaggggag ttaatatact gcaatggttt tgacgccagc ttgaccacca tatcattcgc
     3541 gccgatatgg gtcatgtaag cggcaaatcc acacagcatc ataatcatca tgccgaggtc
     3601 gccgccgcgg ctcattagta atattttaac gtattcaacg atatccgtgg cgctgtagcc
     3661 tgttgaagcc tggctggacg gtaacacttt gtgccccata atggcactga taatcagcaa
     3721 taacaggcca ccgacaaata acacaccagt ggcggaatac cctttaatga tgtagcgagc
     3781 tacacccaca ataaccacaa ccccaataag gagttcaatg aatgtcagca taatttttcc
     3841 tgtctccagg ccccaaagta aataataaaa aattcctaaa gcttaaggaa aaaatatgcc
     3901 caataaattg tcgatgaatg ctgattaaaa tcaagaaaaa ctgc
//
LOCUS       DAFAVF010000250         3916 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFAVF010000250 DAFAVF010000000
VERSION     DAFAVF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.254"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..236
                     /locus_tag="LGK42_004330"
     CDS             <1..236
                     /locus_tag="LGK42_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2660491.1"
                     /translation="KLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASV
                     SYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            372..518
                     /locus_tag="LGK42_004331"
                     /pseudo
     CDS             372..518
                     /locus_tag="LGK42_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(518..1675)
                     /gene="intS"
                     /locus_tag="LGK42_004332"
     CDS             complement(518..1675)
                     /gene="intS"
                     /locus_tag="LGK42_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBK2660492.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1837..1911)
                     /locus_tag="LGK42_004333"
     tRNA            complement(1837..1911)
                     /locus_tag="LGK42_004333"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1876..1878),aa:Arg,seq:cct)
     gene            complement(1987..2919)
                     /locus_tag="LGK42_004334"
     CDS             complement(1987..2919)
                     /locus_tag="LGK42_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK2660493.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3213..>3916
                     /gene="mlaA"
                     /locus_tag="LGK42_004335"
     CDS             3213..>3916
                     /gene="mlaA"
                     /locus_tag="LGK42_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBK2660494.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQE"
BASE COUNT          928 a          948 c          938 g         1102 t
ORIGIN      
        1 ggaaattgaa agaagagggg gtatcattca gtaagatcat acttgatgag aggatgcaaa
       61 tggctgaata tttactcagc actcgttgtt atcctattag taaagtagct aaggtctgtg
      121 gttatgccag tgtctcatac tttacttatg tatttagacg ttattttggt gtttctccaa
      181 gtcaatactc tcagaggagt tcagaaagta aaattcttac tcaccaagga atctgatcat
      241 tgttcttgcc cccttatttc cagacagggg tgtatcttaa gttaacgtta cccgctgacg
      301 tcgatatttg aatcgccacg ggtttaacag acacctcaga gtcatttaag atggcttaaa
      361 gagaggtgcc catgagcggt aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac
      421 aggttgttga tcgcggttat tctgttgcca gcgttgcaac acgtctcgat atcaccaccc
      481 acagccttta tgcctggata aagaagtacg gtccggatta ctccactttt tcatcaagcc
      541 aatccgccca ccactgcatc atttctctgc gcttatcgag atactgagca tggttgtaaa
      601 taccgcgcac agatccgccg ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc
      661 attcgtgctc gttcataatc gtgctgaatt catgcctgaa tccgtgaccg ctttccagac
      721 cttcatagcc gatttgtttg atcacaagca ataccgcgtt ctcgcagatt ggcttcttct
      781 tatcgttgcg cccggcaaaa acaaactctg agactggttt ggtgattgag cttagcgtag
      841 tgagaagttc aaccacctgg tctgacatcg ggaccacatg aattttgcgt cccttcatca
      901 cactggcgtc gatggtgata atcctgtttt caaaatcgac gttcttccat agcatggaac
      961 gaagctcttt cgttcttagg gctgtataac gtaaaacttt tgtcgcaatg agcgatacga
     1021 tacttcctga aaatgttgcc agtgctttgt tgaatgcagg catctggtct gctggaagga
     1081 acgggaagtt cttcttgcgg tatcctttca tggcgtcagc aaggtcaggt gccgggttat
     1141 atttagccct tccggtgaca atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt
     1201 tgttggctcg ctccattgca ccgcgatctt caaatctgcg gattacttcc agcagttgca
     1261 tcggctcaat atcctgaatc tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca
     1321 ttttggcaag ttcagttgca tagcctactg accagacttg cttcttgtgc tcgtaccatt
     1381 ccttgtaaat cgcactaaat gaattgttgt tagacgaagc ctttttcgcc tttaccggat
     1441 cgatgccaac cgagatgtct ttcctcgcag tccatgcctt atcccttgct tcctgcaaag
     1501 tcataagcgg atattttccg acggtcagga ttttctcctt accgtcaatc ttgtagcgaa
     1561 gctgccatac ctttttcccg gatacaggaa cataaaggta caggccatta ccatcgagaa
     1621 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
     1681 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
     1741 acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata ttcaggggat
     1801 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
     1861 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
     1921 tgagtatacc cgctaatgga ctgcggggta agtacgctgc cgctcgattg cttaaaccct
     1981 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
     2041 gcgtgcttct gctttacgct tgttgctcat gtcgttacga atctgtgcat gactcattaa
     2101 cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca
     2161 gatgaaatcg ctccagtgca gcgtgccgtt aaacaccaga tagaggattt caacagaacc
     2221 gacaacgatg tgggtggtgt cacccagtgc aataagccag gtcatcaata taatcaccac
     2281 aatctttgcc gcacccgcag caggaaacat ccaaaccata gtggcgatca gccagccgga
     2341 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
     2401 gacaaatgca tcgcgagttt cttcattgaa gataggcata tattcaaatg cccatgcagc
     2461 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataatcgca taagtaagcc
     2521 gacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     2581 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     2641 cagtaagaag ctgcctggca ctccttccag ttcgacatga aatatccctt ttgccagtag
     2701 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg
     2761 ttccagctct ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg
     2821 gagtcgatct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     2881 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     2941 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     3001 gtagaaaagg ctgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     3061 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     3121 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     3181 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     3241 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     3301 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaacgta ttagacccgt
     3361 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg
     3421 gtttgagcaa ctttactggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     3481 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     3541 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     3601 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     3661 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt
     3721 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     3781 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     3841 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     3901 tcaaaccgca ggaaaa
//
LOCUS       DAFAVF010000251         3901 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFAVF010000251 DAFAVF010000000
VERSION     DAFAVF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.255"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            375..605
                     /locus_tag="LGK42_004336"
     CDS             375..605
                     /locus_tag="LGK42_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBK2660495.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            602..1018
                     /locus_tag="LGK42_004337"
     CDS             602..1018
                     /locus_tag="LGK42_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBK2660496.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            1093..2658
                     /locus_tag="LGK42_004338"
     CDS             1093..2658
                     /locus_tag="LGK42_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK2660497.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            2643..3665
                     /locus_tag="LGK42_004339"
     CDS             2643..3665
                     /locus_tag="LGK42_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HBK2660498.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT          939 a         1028 c         1065 g          869 t
ORIGIN      
        1 actgtttttc tatgatccga tttttatcgg attcaggatt ttccggaagt gaatcaggat
       61 cagtgaataa gcgaagcgat cttttctgcc gctgatgact ggctcgggaa cacgggaaga
      121 aacacaacat acagtgtcat gtcagcggag cgggacacca catcctgtga aagcggacca
      181 gcgggcggca gcgaaggatc cgcggatccg gaaccaccag aaaccggatc tgcacgggga
      241 aagagagaaa ataacatagg ctgaacatat ccatatgcct gacatctgcc tatactaaac
      301 atatgcacac acccggtgca atgaacatca ttccggatat gcatcacata tccacactta
      361 cccgggagag aatcatgaga accgtatcca tttttaaaaa tggcaacaac cgtgctatcc
      421 gccttccacg cgatctggat tttgaggggg tgagcgagct ggagatcgtc cgggaagggg
      481 acagcatcat tctgcgcccc gtccggccga cctggggctc attcctggag ctagaaaagg
      541 ccgatccgga ctttatgacg gagcgcgagg acgttgtcag cgacgaagga cgagttaacc
      601 tgtgaacaag atctatatgc tcgacacgaa catctgctcg ttcatcatgc gcgagcaacc
      661 tgaagcattg cttaagcacc tggagcagtc ggtgctgcgc ggtcaccgta tcgtggtctc
      721 ggccatcacc tactccgaga tgcgcttcgg tgccaccggt ccgaaggcct cgccacgcca
      781 cgtgcaactg gttgacgcgt tctgtgagag gctcgatgcc gtcctgccgt gggaccgcgc
      841 cgcggtggat gcgaccacgg agattaaggt ggcactgcgt ctcgccggca cgccgatcgg
      901 tccgaacgac acggcgatag ccggacacgc catcgctgcc tgtgccattc tggtgacgaa
      961 taatgtgaga gagtttgagc gggtaccggg tcttgtgctg gaagactggg taagataacg
     1021 ctgtaaaaca aaacaggcgg tcaggcgcgg ccggccgctc tttcaggcag cggtatataa
     1081 aggaataaaa aaatgccact gatcacgcgg cttatgctgc aaaacttcaa aaagtttccg
     1141 gaactggacc tgcgcttcag caacgatcgt aacattctgg ttggggacaa tgagtccggg
     1201 aaaagcacta ttctgctggc tcttgacctt gtgctgagtg acagcaggca tcgggttgag
     1261 gcgctggggg tggaatcgct cctgtcacag tcagcagtca ggcattttca ggaaggtgag
     1321 cgccgtgccg atcagttacc cgtgctgacc gctgacgttt tcctgagtaa tgggggagag
     1381 cctgacctga acggcaggca aaatctggcg ggtatcgatg cggatggtct cagaatgcgc
     1441 attgctccca tgatggagga atacggtcag gatatacacc atgttttgca gcaggatcct
     1501 gataattttc cctatgaata ctattccgtg cagttcagca cattctccgg tggtcatttc
     1561 gcaggcttca gacgctatct gcgccatctg ttcttagaca gtgcacgcat tgataacgag
     1621 catgccgcac aggagtatac gcgcacagtt tacagcgtca atgtgccggt ggccgatcgg
     1681 taccggcttg aaaacagtta ccggcaacag aaaatgcact tctgcgcccg gcatctgtcc
     1741 gccataaacg acacgctgaa aacgtatcag tttggcgtgc gttccggcgc taaatcaggt
     1801 cttgaggcga acctggacat tactgaggac ggtatttcca ttcgtcaccg cggaaaagga
     1861 agacagtgtt tcatcaaaac ggagtttgcg ctgcagcggc atcagcagca ggggggagag
     1921 atccacgcgc tgttgcttga ggagccggag aatcatctca gccatgtcag tatgaagagg
     1981 ctggttaatc agcttgccac ggaacggcag acgcaggtgt ttattgccac acacagtagc
     2041 catatctcct cccgcctcga cctacgtaag gctattctcc tcggagctac ccggccggta
     2101 ctgatgaatg aactttccgc tgagactgcc gccttcttca tgaaagcacc ggataataac
     2161 gtgctggaat ttgcgctggc cagacgcgtg ctgctcgtgg agggggatgc tgagtttatt
     2221 ctgatcgagg ccttttatca ccgcctgtat ggcagggcac cggaggatga cggggttcac
     2281 atcatcgcta tcggcggaac gagtttccgt cgttatcttg aactggcccg tcttctggaa
     2341 aatcgtgtcg cggcccttcg cgacaatgac ggcaactatc agcaaaattg cgatgaacgc
     2401 tatgctgacg tgctttgttc ccgctcccgg gtatttgctg accacgacaa cagccgctcc
     2461 acatttgaaa tctgcctgta tcaggataat gccgacctgt gtgatgcgct tttccgcgga
     2521 acccgccgga cactcacggt tcaggattac atgctggcca ataaggcaga agcagcattc
     2581 cagctgttac agttgcacgc cgaaaagctg accgttcctg attatatcca ggaggcactg
     2641 gcatggataa gagagtgata tttgcggttg ccggctcggg taaaaccacg ctgctgatac
     2701 gtcggttaag agaggaccgg cggacgctga tcctgacctt caccgtaaat aatgaagcac
     2761 atctgcgggc gcagattatc agacgctttg gctacatacc gtacgggatc cgtgtgatga
     2821 cctggtttga gtttctgcac ggtttctgtt ttcgcccttt cctgcaggag caactgtcat
     2881 cgcgtggtct gagcttcaac cagccccctt ccagaatacc gcgcacgaat atccggcatt
     2941 atcaggatcc cgccggacgg ctgtatcacc ggcggctcgc acacctgctg acagcccggg
     3001 ggttgctgcc agatatccgt acccgacttg cccgttacta cgatgagttg ttcgttgatg
     3061 aagtccagga ctttgccggc catgacttta attttctgct ggagctatgc cgcgcggaaa
     3121 tctcagtgct gtgctgcggg gatttttatc agcatacctt tgacacaagc cgcgacggta
     3181 acgtcaatgc tacgctgcat gaagacataa cccgatacga ggctcgcttc agggctgcgg
     3241 gtatcatggt ggattgtgag acgctcagcc ggacatggcg ctgttcagct acagtgtgtg
     3301 agttcattac cggacaactg aacattcgca tttcggctca tggaacacat accacccaga
     3361 ttgaaatcgt cacggatgaa gcgcgttcag cggccctgca cgcggacaac acgatgatca
     3421 agctcttcta tcgcgaacat caccgttacg gatgccactc aatgaactgg gggggcagta
     3481 aaggactcga ccactttcag gacgtctgca tcgtgatggg ggccaaccac tggatgcgcc
     3541 tcatccaaca gaagctggca gcacttccac catcatcccg taacaggcta tacgtggcct
     3601 gctcacgggc acggggcaac atctatttta ttccggaaag ccatcttcgg agattcagaa
     3661 actaagtcag cggcttctca actgggcaca agccggtaaa aagtagtcaa cgacaaaact
     3721 tcaggccgaa aaatgaggct ccggtttcgc cggaaaccgg acctgcgtgg agggaaaccc
     3781 gcagatgata tgatatttat cattaatcgc ataataaatc ctggcctgat atttacaggc
     3841 agagaaataa atcattcgat atatgatttt atcgtcatca gccattaacc ccccaggaag
     3901 c
//
LOCUS       DAFAVF010000252         3814 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFAVF010000252 DAFAVF010000000
VERSION     DAFAVF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.256"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..138)
                     /locus_tag="LGK42_004340"
     CDS             complement(<1..138)
                     /locus_tag="LGK42_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK2660499.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RK"
     gene            296..1309
                     /gene="intI1"
                     /locus_tag="LGK42_004341"
     CDS             296..1309
                     /gene="intI1"
                     /locus_tag="LGK42_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBK2660500.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1512..1862
                     /locus_tag="LGK42_004342"
     CDS             1512..1862
                     /locus_tag="LGK42_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBK2660501.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2038..2598
                     /locus_tag="LGK42_004343"
     CDS             2038..2598
                     /locus_tag="LGK42_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2660502.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            2602..>3814
                     /locus_tag="LGK42_004344"
     CDS             2602..>3814
                     /locus_tag="LGK42_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HBK2660503.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
BASE COUNT          745 a         1193 c         1171 g          705 t
ORIGIN      
        1 ttttcttccg acaaggagcc attgattata tgtgagcgct ttaaagagta gttgctcacc
       61 ttttactgac cacgggatat caggaccact accgattacg ccattttctg acactgcaga
      121 aatcaatgat attttcaatt taactccctt aatggctaac tttgttttag ggcgactgcc
      181 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      241 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      301 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      361 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtcc actgggttcg
      421 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga
      481 ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc
      541 attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca
      601 ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt
      661 ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc ttctgtatgg
      721 aacgggcatg cggatcagtg agggtttgca actgcgggtc aaggatctgg atttcgatca
      781 cggcacgatc atcgtgcggg agggcaaggg ctccaaggat cgggccttga tgttacccga
      841 gagcttggca cccagcctgc gcgagcagct gtcgcgtgca cgggcatggt ggctgaagga
      901 ccaggccgag ggccgcagcg gcgttgcgct tcccgacgcc cttgagcgga agtatccgcg
      961 cgccgggcat tcctggccgt ggttctgggt ttttgcgcag cacacgcatt cgaccgatcc
     1021 acggagcggt gtcgtgcgtc gccatcacat gtatgaccag acctttcagc gcgccttcaa
     1081 acgtgccgta gaacaagcag gcatcacgaa gcccgccaca ccgcacaccc tccgccactc
     1141 gttcgcgacg gccttgctcc gcagcggtta cgacattcga accgtgcagg atctgctcgg
     1201 ccattccgac gtctctacga cgatgattta cacgcatgtg ctgaaagttg gcggtgccgg
     1261 agtgcgctca ccgcttgatg cgctgccgcc cctcactagt gagaggtagg gcagcgcaag
     1321 tcaatcctgg cggattcact acccctgcgc gaaggccatc ggtgccgcat cgaacggccg
     1381 gttgcggaaa gtcctccctg cgtccgctga tggccggcag cagcccgtcg ttgcctgatg
     1441 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     1501 tgaaaacgac aatggaggtg gtagccgagg gtgtggaaac acccgactgc cttgcgtggt
     1561 tgcggcaggc gggttgcgac acggtgcagg gtttcctgtt cgccaggccg atgccggcgg
     1621 cggccttcgt cggcttcgtc aaccaatgga ggaacaccac catgaacgcc aatgaaccga
     1681 gcaccagttg ctgcgtgtgc tgcaaggaaa tcccgctcga tgccgccttc acgccggaag
     1741 gggccgagta cgtggagcat ttctgcgggc tggagtgcta tcagcgcttc caggcgcggg
     1801 ccagcactgc gaccgaaacc agcgtcaaac cggacgcttg tgattcgccg ccgtcaggtt
     1861 gaggcatacc ctaacttgat gtcaggcagg gccgcgccgc ttcgtcagaa tagagtctgc
     1921 tttcccattt tttgacacat gcccgcgaag gttatagatt tcagcctgac agaaatgggc
     1981 tttgaggcac aacggaacag aaagtgcact taagccgcct tcaaccaagg agacatcgtg
     2041 caggggcacc gcatcggcta cgtccgggtc agcagctttg accagaaccc ggaacgccag
     2101 ctggaacaaa cccaggtgag caaggtgttc accgacaagg catcgggcaa ggacacccag
     2161 cgcccccagc tcgaagcgct gctgagcttc gtccgcgaag gcgatacagt ggtggtgcac
     2221 agcatggatc ggctggcccg caacctcgat gacctgcgtc gcttggtaca gaagctgact
     2281 cagcgcggcg tgcgcatcga gttcctgaag gagggcctgg tgttcactgg cgaggactcg
     2341 ccgatggcca acctgatgct gtcggtgatg ggggccttcg ctgagttcga gcgcgccctg
     2401 atccgcgagc ggcagcgtga gggcatcgcc ttggccaagc agcgtggcgc gtaccggggc
     2461 cgcaagaaag ccctgtccga tgagcaggct gctaccctgc ggcagcgagc gacggccggc
     2521 gagcccaagg cgcagcttgc ccgcgagttc aacatcagcc gggaaaccct ctaccagtac
     2581 ctccgcacgg acgactgaca catgccgcgt cgcttgatcc tctcggccac ggagcgggac
     2641 accctgcttg cgctgccgga aagccaggat gacctgatcc gctactacac cttcaacgac
     2701 tccgacctgt cgctgatccg ccagcgacgc ggcgacgcca accgcctcgg cttcgccgtg
     2761 cagctctgcc tgctgcgcta ccccggttac gcgctgggaa ccgacagcga gctgcccgag
     2821 ccggtcatcc tgtgggtggc gaagcaagtc caggccgagc cggcgagctg ggcaaagtac
     2881 ggcgagcgcg acgtgacccg tcgcgagcat gcccaggaac tgcgcaccta cctgcaactg
     2941 gccccgttcg gcctgtccga cttccgcgcc ctggtgcgcg agctaaccga gctggcccag
     3001 cagaccgaca aaggcttgct gctggccggt caggccctgg agagcctacg gcagaaacga
     3061 cgcatcctgc cggcgctgag cgtgattgac cgggcctgct cggaagccat tgcgcgagcc
     3121 aatcggcggg tctaccgcgc cctggtcgaa ccactcacgg actcgcatcg ggccaagctg
     3181 gacgagctgt tgaagctcaa ggccggcagc agcatcacct ggttgacctg gctgcgccag
     3241 gcaccgctga aacccaactc tcggcacatg ctggaacaca tcgagcggct gaagacattt
     3301 cagttggtgg acttgcccga aggcctgggc cggcacatcc accagaaccg cctgctcaag
     3361 ctggcccgcg agggtgggca gatgacgccc aaagacctcg gtaagttcga gccgcagcgc
     3421 cgctacgcga ccctggccgc cgtggtgctg gagagcaccg cgaccgtgat cgatgagctg
     3481 gtcgatctgc atgaccgcat cctggtcaag ctgttcagcg gcgcgaagca caagcatcag
     3541 cagcagttcc agaagcaggg caaggcgatc aacgacaagg tgcgcctgta ctccaggatc
     3601 ggccaggcgc tgctggaagc gaaggaaagc ggcagcgacc cctatgccgc catcgaggcg
     3661 gtgattccct gggacgagtt caccgagagc gtcagcgagg ccgagctgct ggcccggccg
     3721 gaaggcttcg accacctgca cctggtcggc gagaacttcg ccaccctgcg ccgttacacg
     3781 ccggccttgc tggaggtgct ggaactgcgc gccg
//
LOCUS       DAFAVF010000253         3789 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFAVF010000253 DAFAVF010000000
VERSION     DAFAVF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.257"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            48..323
                     /gene="ypdI"
                     /locus_tag="LGK42_004345"
     CDS             48..323
                     /gene="ypdI"
                     /locus_tag="LGK42_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBK2660504.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(400..642)
                     /locus_tag="LGK42_004346"
     CDS             complement(400..642)
                     /locus_tag="LGK42_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBK2660505.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            995..1915
                     /gene="lpxP"
                     /locus_tag="LGK42_004347"
     CDS             995..1915
                     /gene="lpxP"
                     /locus_tag="LGK42_004347"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBK2660506.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2271..2342
                     /gene="ypdK"
                     /locus_tag="LGK42_004348"
     CDS             2271..2342
                     /gene="ypdK"
                     /locus_tag="LGK42_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBK2660507.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2407..3645)
                     /gene="alaC"
                     /locus_tag="LGK42_004349"
     CDS             complement(2407..3645)
                     /gene="alaC"
                     /locus_tag="LGK42_004349"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBK2660508.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          982 a          963 c          885 g          959 t
ORIGIN      
        1 ccataacatc gtaggttctc agtgaaccat tttcaggata actttctatg aaagtaaact
       61 taatactttt cagcttattt ttattggtct ctattatggc atgcaatgtt tttgcatttt
      121 ccatttctgg tggtgtaagt gagaggagct ataaagagac tgaaaaaaca tcagcgatga
      181 caaccacaca ctctacaaaa cttcagccat cacaggcgat tttgtttaag atgagagaag
      241 atgcgccacc attaaacctc acagaagaaa tcacgcctac ttatccgaca aaggcgaatt
      301 atcttattca tcctgtgcga tagcgtctgt cacatggcag gtgaacaatc atgtctgatg
      361 ctgatgtgaa cacctgccca aatgagccat cctgacccac tatcgcctgc cataccccac
      421 cataaagcga atcatcaagc cacaagccgg ggcaagaaac ataaatatcc ccgtcactag
      481 ttcattaccg ccagttaacc acatggtgaa gtaataaatg agtgcagcga ggatcaccat
      541 gccgaagaaa gaagagaccg ccgataccac gttcagtacc tgaccgatgt agccgcttac
      601 gcagacaata cacagggcga gaaacatcca taaataaatc atagctcaac ctcctgccgc
      661 gctaaaaaac ctgtaacttc agtcacttta atagtaagac aatccttaac ctctggcaat
      721 ttttgctaag catcaatgaa aacagcatgt taaatgcaag actgttgtgt acggaaaaat
      781 atttactttg cacgattaat aatcagtagc tgaaagcagt cagcgagtga taaagtacag
      841 ataccgcatt catgctcctc gtggttatgt cctgactagt ctttacactc tttacaggaa
      901 ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct
      961 gcgatacaac aattcgtatc tacagaaggt aactatgttt ccacaatgca aattttcccg
     1021 cgagtttcta catcctcgct actggctcac atggtttggg cttggtgtac tctggctttg
     1081 ggtacagctt ccttatcctg ttctctgctt tctcggcacg cgcattggcg caatggcgcg
     1141 accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac ctggaacttt gtttcccgca
     1201 gcattctgcg gaagaacgcg agaagatgat tgccgaaaac tttcgttcac tcggcatggc
     1261 gctggtagaa accggcatgg catggttctg gcccgacagt cgcgtacgta aatggtttga
     1321 tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt
     1381 cggcgtccat tttatgtcgc tggaactggg cggccgcgtg atgggactgt gccaaccaat
     1441 gatggctacc tatcgtccac ataataatca gctgatggaa tgggtgcaga cccgtgggcg
     1501 catgcgctct aacaaagcga tgatcggcag aaataatctg cgcggcattg tcggtgcact
     1561 gaagaaaggt gaagcggtat ggtttgctcc cgatcaggat tatggtcgta aaggcagctc
     1621 cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc aatggcacct atgttctctc
     1681 ccgtctctct ggcgcagcca tgttgaccgt aacgatggta agaaaagcgg attacagcgg
     1741 atatcgtttg ttcatcaccc cagagatgga aggctacccg acagatgaaa atcaagccgc
     1801 tgcctatatg aacaagatta tcgagaaaga gatcatgcgc gcaccggagc agtacctctg
     1861 gatccaccgt cgctttaaaa cgcgcccggt gggagaatcg tcgttgtaca tttaacaaac
     1921 tgctcttact ctttccctcc tcaaatcggc cataataacc tcatggtttt tatggctttt
     1981 ttcttaaggt tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg
     2041 ccgatatgag acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt
     2101 cagttttagg cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg
     2161 cccacggtaa aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag
     2221 gtgtaatgaa gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt
     2281 tctttatggg catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct
     2341 aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga
     2401 cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc
     2461 acggaacatc gctttaatcc cacgaatcgc ctgacgaata cggtcgcggt tttcaatcag
     2521 tgcaaagcga acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac
     2581 cttcgcttcg ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc
     2641 cgggattttc gcccagacat acatcgaagc cttcggcatt tcaaccatcc agcccgcttc
     2701 atgcagccct ttaaccaata catcacggcg gcgtttgtac tgttcggcaa tgtcgcgcac
     2761 gcactgttga tcgccctcca gcgccgcaat cgccgccacc tgcaacggcg taaaggtgcc
     2821 gtaatcgtga tagcttttaa tacgagccag ggcgctgacc agcgttttgt tgccaaccat
     2881 aaagccgata cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgactgc
     2941 cacatcgcgt gcacccggta cctgcatgat tgacggcgct ttccagccat cgtagacgat
     3001 atcggcatag gccaggtcgt ggaccaccag cacatcgtag cgtttcgcca gcgccacaac
     3061 cttttcgaag aactccagct ccacgcattg cgcggttgga ttcgacggga agccgaggat
     3121 catcatcttc ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc
     3181 gacaccttcc accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat
     3241 atggatcggg taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag
     3301 catcagatgc gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc
     3361 gatttcaacg tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat
     3421 gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc
     3481 gacgatatgc ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc
     3541 gccgcgccga cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag
     3601 acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca gccataattt cctcagatta
     3661 acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc
     3721 ctgattaatt tctgtctgtc cagcgtttta gaacattttt atcaatggct ttaaatgagg
     3781 aactccact
//
LOCUS       DAFAVF010000254         3788 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFAVF010000254 DAFAVF010000000
VERSION     DAFAVF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.258"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1011
                     /locus_tag="LGK42_004350"
     CDS             <1..1011
                     /locus_tag="LGK42_004350"
                     /inference="COORDINATES: protein motif:HMM:NF013890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660509.1"
                     /translation="RWEDFAFNEIHTLIRALNITQNNPLNVNAPELRYLAALFAEMSY
                     HLVPEWEVDKHKRAKLYRVPSEGYQILVDQGIRGSENVFEAFIDAELPKPFIASSAGI
                     VVVGVVIDGIMFIGFRGTALLFDWKVNLRAELVRVDSAYRVTKYHWFDHCHIEDKKII
                     SGGLHSGFAEEAVRITKRIFDAIPEADRSSIEHVVLAGHSLGGAVAAVSKSLITQWPA
                     TACIFGAPRYADAGFYLNCFDNYPIHIRRLGDLVPTVPPRSYGFCDHPNEFATNGEEY
                     IDTSLNSWLVSDLCNWTRFLSTKTAAHSMENYRTEIGKAIGSQFADKPLTKAVKITKR
                     HI"
     gene            1420..2193
                     /locus_tag="LGK42_004351"
     CDS             1420..2193
                     /locus_tag="LGK42_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660510.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEIILATVRGIVRGVFIADKWLKSTRENFPSLKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(2263..2391)
                     /locus_tag="LGK42_004352"
     CDS             complement(2263..2391)
                     /locus_tag="LGK42_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2660511.1"
                     /translation="MDIATEEETLLQEAWKKYRVLLNRVDTSTAPDIEWPEEPDTV"
     gene            complement(2446..>3788)
                     /locus_tag="LGK42_004353"
     CDS             complement(2446..>3788)
                     /locus_tag="LGK42_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBK2660512.1"
                     /translation="DYDDAGSVSNAIAYNFANDRIDVQNLKASGLITANSGIATLTGH
                     DWNSQHTDNVDKFRPIAGSTNGPAGSMVLGGIHVQFSKNYAVQFGGRNSGFWGRTVEN
                     GTTQEWKKLLTVDDLNSSTDLAVRSLTTSNPLKSGGGRIDVLGSTSDYSKMDCFVRGF
                     DSTGNSLAWALGSSVGVSKMLSLKNFFSGAEILLNGNDGAVQLKTGAVNGAKAQALTI
                     NKDEVNSTVDLTLTKQSGTGNRFVLQNLGNTELSFAAKVWGSSDRQNVFEVGTSAAYL
                     FYAQKTSAGQLFDVNGAINCTTLNQSSDRDLKDDIRVISDAMKAIRKMNGYTYTLKEN
                     GMPYAGVIAQEVMEAIPEAVGSFTHYGEELQGPTVDGNELREETRYLNVDYSAVTGLL
                     VQVARETDDRVTALEEENTTLRENLATADTRITTLENQVSELVALVRQLTGSEH"
BASE COUNT         1036 a          858 c          835 g         1059 t
ORIGIN      
        1 aggtgggaag atttcgcttt taatgaaatt cacacgctga tccgagctct caacataaca
       61 cagaacaacc cgcttaatgt gaatgctccg gaactgagat accttgccgc gctttttgcc
      121 gaaatgtctt atcacttggt accagaatgg gaagtggata agcataaacg cgcaaagctc
      181 tacagagtcc cgagtgaagg ctatcagata ttggtcgacc aaggtatccg gggcagtgaa
      241 aatgttttcg aagctttcat tgatgcggaa ctaccaaaac ccttcattgc gtcatctgca
      301 gggattgtag tggttggagt ggtaatcgat gggattatgt tcattggatt ccgtggtacg
      361 gcactgctgt tcgactggaa ggttaactta cgtgcagagc ttgtgcgcgt ggattccgct
      421 taccgcgtaa caaaatatca ttggtttgac cattgccaca ttgaagataa aaaaattata
      481 tctggtggat tgcatagtgg ttttgcggaa gaagccgttc ggataacaaa gcgcattttt
      541 gacgcaatac ctgaagcaga ccgttcatct atcgagcatg ttgtactcgc tggccattca
      601 ctgggcggag ccgtagcggc agtatcgaag tctctgataa cacaatggcc agctacggcg
      661 tgtatattcg gtgcaccaag gtatgctgat gctggtttct atctgaattg tttcgacaat
      721 tatccaatcc atatacgccg acttggtgat ttagtaccaa ccgttccccc acgttcatat
      781 ggtttctgcg accaccctaa tgagttcgcc accaacggtg aagagtacat agacacaagt
      841 ttaaattcat ggcttgtgag tgatctatgc aattggacca gatttttgag tacaaaaacc
      901 gctgctcata gcatggagaa ttatcgcacg gagataggca aggcgatagg atctcagttc
      961 gcagataaac ctctgacaaa ggcagtaaaa attacgaaaa ggcacatatg acctatcaac
     1021 acaggctgtt gttagctact tccgcttttg gcacatagca gccctagaga cagtggcgta
     1081 aagtcatgga gaatcggtgg gaagatattc agtttcattc atgccgaaat acggtaaaat
     1141 ctataacaga tagaaatcat tcaatattcg cactattgga agttcaccag ccagccacag
     1201 cacgttcttg catacaacgt gcctgcggtt tttgagaccg gtccgatcat caaacgaaac
     1261 ataaaattag ctcacactat gaggaaaagt atcttttttt actttgtaaa ttcaaaggat
     1321 tagcctcatt tacccgatag ttttctcaac actactagtt gtgagccctt gcaatgttca
     1381 ttaatatacg tctcacaaat aattcataga tattgcaaaa tggatattac tgagtttcct
     1441 tctggagtaa ttgaacacct tggctggtat gtataccgat tgattgatcc gagggacgga
     1501 agcaccttct atgtagggaa aggcaaaggt aaccgcgtat ttgcccatat gcgcggtgaa
     1561 gtggcagcga ctgatgatga cgagttactg agcaacaagc taaagcaaat tagagaaata
     1621 aggttagcag gacttgaagt tatccatgtc atccatcgac acggaatgac tgatgaaaag
     1681 acggcgtacg aagttgaagc agcacttatt gatgcctacc ctgggttaac gaatatcatg
     1741 aatggtgctg gcagcaatga attcggcgcc gcgcatgtca aagagttgat agcaacatat
     1801 cagcccgaaa ccataacatt tcatcataaa gcattaatga tatccgttaa cagaagtgca
     1861 aaggattcag agctttatga tgcggttcga tttagctggc gcattaatgt ctctcgcgcc
     1921 agccaagcag aaatcattct tgctactgta agggggatcg ttcgaggggt tttcattgct
     1981 gataaatggc tcaaatcaac acgtgaaaat ttcccttcgt tgaaatactg ggacgaggat
     2041 cctgactttg aggcaacaca aagttcgcgc tatggttttg aaggtcgaga agccccacct
     2101 gaaatagcaa atctttatct tggaaaaaaa ataccagatg aattaagaaa aaaaggagct
     2161 atgtccccgg tccgttactc acctaatttt tgagtcttta agtgataagc ataaaccgca
     2221 gcacgtcatg catacgtcgt gtctgcggtt tttctttttt acttacacgg tgtctggttc
     2281 ttctggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca acacccgata
     2341 cttcttccag gcttcctgca acaaggtttc ttcctccgtt gcgatatcca gctcaacaac
     2401 agtctgaacg taccgggaac agcctccttc agggcttgaa ggatatcaat gttcgcttcc
     2461 tgttaactgc cggacaagtg caaccagttc gcttacctga ttttccagag tggtgatccg
     2521 ggtgtctgct gttgccagat tttcacgtag cgttgtgttt tcctcttcca gcgcagtaac
     2581 tcgatcatct gtttcacggg cgacctgaac aagtaacccc gtcacggcgg agtagtcaac
     2641 attaagatag cgagtttctt cgcgtagctc gttgccgtca acggtcggac cttgcaactc
     2701 ttcaccataa tgagtaaacg atcccacagc ttcaggtatc gcctccatta cttcctgtgc
     2761 aataacgcca gcataaggca ttccgttttc cttgagcgtg taggtgtacc cgttcatttt
     2821 acggattgct ttcatcgcgt cgctgataac gcgaatatcg tctttaaggt cgcggtctga
     2881 tgactgattc agcgttgtgc aattaatagc gccatttaca tcaaacaact ggcctgctga
     2941 tgttttttgc gcataaaaca gatatgcagc agacgttcca acctcaaaaa cgttttgtcg
     3001 atcacttgat ccccatactt ttgcggcaaa tgatagttct gtattaccta agttctgtaa
     3061 aacaaaacga ttgccagtcc ctgattgttt tgtaagggtt aaatcaacag ttgagttaac
     3121 ctcatccttg ttgatagtga gcgcctgcgc tttagccccg ttaacagcac ctgttttgag
     3181 ttgaaccgcg ccgtcattac catttagcag tatctcagct ccgctaaaga aattttttag
     3241 cgacagcatc ttacttacgc cgactgatga acccaacgcc cacgcgagag aattaccggt
     3301 gctatcaaac ccacgtacaa agcaatccat tttgctatag tctgacgtgc ttccaaggac
     3361 atcaatccgc cctccgccag attttaacgg gttagatgtg gttaatgacc tgacagcaag
     3421 atcggtggat gaatttagat cgtctactgt tagtaatttc ttccattcct gcgtcgttcc
     3481 gttttcaact gttcttcccc aaaaaccgga attgcggcct ccgaactgca cagcataatt
     3541 tttactaaat tgaacatgaa tgccgccaag aaccatagaa cctgccgggc cgtttgtact
     3601 gcctgcgatt ggtctaaatt tgtcgacgtt atcagtatgc tgagagttcc agtcatgacc
     3661 tgttaatgtt gcgattcctg agttagctgt aattaaccca gacgctttaa gattttgcac
     3721 atcaattctg tcgtttgcga aattatatgc aattgcgtta ctaacgcttc cggcatcatc
     3781 ataatcgc
//
LOCUS       DAFAVF010000255         3746 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFAVF010000255 DAFAVF010000000
VERSION     DAFAVF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.259"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..369)
                     /locus_tag="LGK42_004354"
     CDS             complement(<1..369)
                     /locus_tag="LGK42_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HBK2660513.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNL"
     gene            684..2000
                     /gene="shiA"
                     /locus_tag="LGK42_004355"
     CDS             684..2000
                     /gene="shiA"
                     /locus_tag="LGK42_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBK2660514.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            2102..3556
                     /gene="amn"
                     /locus_tag="LGK42_004356"
     CDS             2102..3556
                     /gene="amn"
                     /locus_tag="LGK42_004356"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBK2660515.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          903 a          886 c          869 g         1088 t
ORIGIN      
        1 caggttcttt ttacgcatcg cctctataca aagatccggc gtaccgtatt gcagtgttat
       61 attcttcgct aaatctttta tttcttttaa tgttgcgtga tcctgcatag tcattgtgac
      121 taatgttaat ttagtctgtt caagtttgag cgcattaaag acttctaaat taattgtcga
      181 cgtaacaatt aatagatgct taattttatg caattcaagc gcccgaataa caggaaagat
      241 ggccatagca tcgccaatct gatcgggaat atgtatgaca acaaagtctg ttttttcaat
      301 attaaaatta taagctttat aatcgtagta actaaatgca atacgtctca acaatgatac
      361 taaaaacata actaacctcg cctccctact ggttataatg caatgcagtc tatcagaatc
      421 atcagggtgc aattttgtgc atatgcggac ttttatgttt catatctcga acctgtgggc
      481 catctgctta atccttaaac aacaccagca actcctgcgc tttcatcttc catcgaattt
      541 ttcatgttgc cgctaatcag ccataaaatc atttgcagat gcgctctgtc gaggtagtct
      601 cataaggttc gtttatagat cgacggcaat gtgagttacc ttttccatac taattataaa
      661 aagacagtac aaacaggatc attatggact ccacgctcat ctccactcgt cccgatgaag
      721 ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg cagtttcgct ggtgccgtcg
      781 tcgactggta tgatttttta ctctatggca ttaccgccgc actggtgttt aatcgcgagt
      841 ttttcccgca agtaagcccg gcgatgggaa cgctcgccgc atttgccacc tttggtgtcg
      901 gcttcctttt ccgaccgctc ggcggtgtca ttttcggtca ctttggtgac cggctgggac
      961 gtaagcgcat gttaatgctg accgtctgga tgatgggcat cgcgacagcc ttgattggta
     1021 ttcttccttc attctcgacc attgggtggt gggcacctat tttgctggtg acactgcgtg
     1081 ccattcaggg atttgccgtc ggcggcgaat ggggaggcgc agcgttgctt tccgttgaaa
     1141 gtgcgccgaa aaataaaaaa gccttttaca gtagcggtgt acaagttggc tacggagtag
     1201 gtttactgct ttcaaccgga ctggtttcat tgatcagtat gatgacgact gacgtacagt
     1261 ttttaagctg gggctggcgc attcctttcc tgtttagcat cgtactggta ctgggagcat
     1321 tgtgggtgcg caatggcatg gaggagtccg cggaatttga acaacagcaa cataatcaag
     1381 cggccgcgaa aaaacgcatc ccggttatcg aagctctgtt acgacatccc ggtgctttcc
     1441 tgaagattat tgcgctacga ctgtgcgagt tgctgacgat gtacatcgtt accgcctttg
     1501 cacttaatta ttcaacccag aatatggggt taccgcgcga acttttcctt aatattggtt
     1561 tgctggtagg tggattaagc tgcctgacaa ttccctgttt tgcctggctt gccgatcgtt
     1621 ttggtcgccg cagggtttat atcacaggtg cgttaatcgg aatgttgagc gcatttcctt
     1681 tctttatggc gcttgaagca caatctattt tctggatagt tttcttctcc ataatgctgg
     1741 caaacattgc gcatgacatg gtggtgtgtg tgcaacaacc gatgtttacc gaaatgtttg
     1801 gtgccagtta tcgctatagt ggcgctggag tcggttatca ggttgccagt gtggttggtg
     1861 gtggatttac accttttatt gccgctgcac tcatcactta ctttgccggg aactggcata
     1921 gcgtcgccat ttatttgctg gctggatgcc tgatttccgc aataaccgct ttgttgatga
     1981 aagacaatca acgcgcttga tagcctggcg aagatcatcc gatcttcgcc ttacactttt
     2041 gtttcacatt cctgtgacat actatcggat gtgcggtaat tgtatggaac aggagacaca
     2101 catgaataat aagggctccg gtctgacccc agctcaggca ctggataaac tcgacgcgct
     2161 gtatgagcaa tctgtagttg ctttacgcaa cgccattggc aactatatta caagtggcga
     2221 attacctgat gaaaacgccc gcaaacaagg tctttttgtc tatccatcac tgaccgtaac
     2281 ttgggatggt agcacaacca atccccctaa aacgcgcgca tttggtcgtt ttactcacgc
     2341 aggcagctac accaccacga ttactcgccc tactctcttt cgttcgtatc tcaatgaaca
     2401 acttacgttg ctgtatcagg attatggtgc gcatatctcg gtgcaaccct cgcagcatga
     2461 aatcccttat ccttatgtca ttgatggctc tgaattgaca cttgatcgct caatgagcgc
     2521 tgggttaact cgctatttcc cgacaacaga actggcgcaa attggcgatg aaactgcaga
     2581 cggcatttat catccaactg aattctcccc gctatcgcat tttgatgcgc gccgcgtcga
     2641 tttttccctc gcgcggttgc gccattatac cggtacgcca gttgaacatt ttcagccgtt
     2701 cgtcttgttt accaactaca cacgttatgt ggatgaattc gttcgttggg gatgcagcca
     2761 gatcctcgat cctgatagtc cctacattgc cctttcttgt gctggcggga actggatcac
     2821 cgccgaaacc gaagcgccag aagaagccat ttccgacctt gcatggaaaa aacaccagat
     2881 gcccgcgtgg catttaatta ccgccgatgg tcagggtatt actctggtga atattggcgt
     2941 gggaccgtca aatgctaaaa ccatctgcga tcatctggca gtactacgcc cggatgtctg
     3001 gttgatgatt ggtcactgtg gtggcttacg tgaaagccag gccattggcg attatgtact
     3061 tgcacatgct tatttacgcg atgatcacgt tcttgatgcg gttctgccgc ccgatattcc
     3121 tattccaagc attgctgaag tgcaacgtgc gctttatgac gctaccaagt tagtcagcgg
     3181 caggcccggt gaggaagtca aacagcggct gcgtacaggt actgtggtaa ccacagatga
     3241 caggaactgg gaattacgtt actcagcttc tgcacttcgt tttaacttaa gccgggccgt
     3301 ggcaattgat atggaaagtg caaccattgc cgcgcaagga tatcgtttcc gcgtgccata
     3361 cgggacacta ctgtgtgttt cagataaacc gttgcatggc gagattaaac ttcccggcca
     3421 ggctaaccgt ttttatgaag gcgctatttc cgaacacctg caaattggca ttcgggcgat
     3481 cgatttgctg cgcgcagaag gcgaccgact gcattcacgt aaattacgaa cctttaatga
     3541 gccgccgttc cgataataag ttatgaacct tcttatcaga aatagatgtg aaggattaag
     3601 taagacatct ggcaaatagc ctgcaataaa cgcgggctgt ttgcttcctt gggcagatgc
     3661 gagttttatt atcgtcttaa tgatttccac atattaaaag caagtatgct ttcaaaacac
     3721 aattataaaa aatcccgcca acaaca
//
LOCUS       DAFAVF010000256         3716 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFAVF010000256 DAFAVF010000000
VERSION     DAFAVF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.260"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..515
                     /locus_tag="LGK42_004357"
     CDS             <1..515
                     /locus_tag="LGK42_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660516.1"
                     /translation="SLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPD
                     VKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSE
                     LYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKK
                     MVDEYQQNSG"
     gene            complement(761..1456)
                     /locus_tag="LGK42_004358"
     CDS             complement(761..1456)
                     /locus_tag="LGK42_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBK2660517.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1449..2876)
                     /locus_tag="LGK42_004359"
     CDS             complement(1449..2876)
                     /locus_tag="LGK42_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBK2660518.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHELLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITEKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHLAQEKKNG"
     gene            complement(2887..3606)
                     /locus_tag="LGK42_004360"
     CDS             complement(2887..3606)
                     /locus_tag="LGK42_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBK2660519.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          971 a         1007 c          786 g          952 t
ORIGIN      
        1 acagcctaaa aataaaccag acaacaccag gcatacttgt taactgtgat agcaataata
       61 actttgcctg tcagaccaca atgactgaag atgttataca gcgaattact acattttttc
      121 acaccagccc agatgtcaag aacagagaaa taaagctgga atggtcagga gataagagag
      181 atttaccaac tgctgaagcg gaaatatctc gcgtgcaggc gtctattatc aaatggtatg
      241 cgtcagaata ccataatggc aggcaagttc tcgatgagat acaaacacct tcagcaatta
      301 acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg tatccgaacg
      361 gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct gctgccattt
      421 gcctggctct gggatttttt ctaagcattg taatttctgt aatgttttgc cttgtcaaaa
      481 aaatggtaga tgaataccaa caaaactctg ggtagtaata aacagcattc tgatagagag
      541 tgcctgtata aagctgaagt gacaatgacc gggaagggaa cccgttttca ggaatggatg
      601 cctgtctttt tcactgtaaa tatcctggca ttactaatgc caggatattt acagaagagc
      661 agcataacca tgacggaacg gggttaagtc aggttgtcct aaacgctatt ttcaacctcg
      721 tatgccttct tcaggtttat gtccagactt catatctctc tcaacaatcc tcaataatca
      781 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      841 ttgaactagg gccgctaatg atgttaatgc aggacggcat tcgttcaccg gcctgcgctt
      901 tctgatgcaa tttttctgcg agttgcgcta cacgcggcaa gatagtgctt ttacgcagga
      961 taaaaagaga agattccggg agcaggctca atgaacgccc gcgcccggcg gcggaaaaaa
     1021 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
     1081 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
     1141 cctgctgcaa acgttcgcta atccccaatt cctctagcct cgtgtcaccg ctaatcacga
     1201 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
     1261 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
     1321 gctgctgttg gttaagtgcg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
     1381 ctgcttgcgg ttcaagtcgc aacgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
     1441 cgctccgatt atccattctt tttctcctgc gccagatgtt tcttaaacca actacggaaa
     1501 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcactaat cgcgccaaat
     1561 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcacc ggccatcatc
     1621 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1681 tctgcttttt cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1741 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1801 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1861 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1921 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1981 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     2041 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     2101 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     2161 atcactgcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     2221 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     2281 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     2341 tccggcccct catagcccag aagttcgtgt agcactcgac ggatctgatg gcgatcttta
     2401 tgaattgccg ggaccacaac atgagagggc ggatcttgat ccagctggag aatgtattca
     2461 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     2521 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     2581 tgtaaaatgt agcgagtggc gtcttcttta gtttttgcaa aatagacgtg accgccgttt
     2641 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2701 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2761 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2821 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2881 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2941 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     3001 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     3061 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     3121 cagctccagt ccacgcacat ttttcagcag cgtaagtggt tcgtccttca cccccagctt
     3181 acgggccagg ctacaagatg ggtgatacac cgctctccct tgcaaactgg cacctacatc
     3241 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     3301 ttcggctcgt gatgcccatt caggttcatc cgccagatac atcgggtaac tttttacggc
     3361 ataggtgcag gagccagccg gtgaaataat gggatcatcg ttatcctcca gtgcggcgat
     3421 cagatttttc atccctggaa tcgcttcttt gatataacca ctattgatcg caggctgacc
     3481 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     3541 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     3601 attcacaact aatactccat tacttcatgc ccatttatgc ccgatgtttc ccccccaccg
     3661 ggggatcaat catcaataaa tatggcgttc ttttttaatt ggctagcaac atacca
//
LOCUS       DAFAVF010000257         3663 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFAVF010000257 DAFAVF010000000
VERSION     DAFAVF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.261"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..957)
                     /locus_tag="LGK42_004361"
     CDS             complement(<1..957)
                     /locus_tag="LGK42_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK2660520.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVIL"
     gene            1064..1360
                     /locus_tag="LGK42_004362"
     CDS             1064..1360
                     /locus_tag="LGK42_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2660521.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1494..1919)
                     /gene="arsC"
                     /locus_tag="LGK42_004363"
     CDS             complement(1494..1919)
                     /gene="arsC"
                     /locus_tag="LGK42_004363"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBK2660522.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1932..3221)
                     /gene="arsB"
                     /locus_tag="LGK42_004364"
     CDS             complement(1932..3221)
                     /gene="arsB"
                     /locus_tag="LGK42_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBK2660523.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3275..3628)
                     /gene="arsR"
                     /locus_tag="LGK42_004365"
     CDS             complement(3275..3628)
                     /gene="arsR"
                     /locus_tag="LGK42_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBK2660524.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
BASE COUNT         1026 a          941 c          876 g          820 t
ORIGIN      
        1 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
       61 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      121 cattcctttt gtcatcagaa cagatgccag agggataact gcctcagccc ggatatacag
      181 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      241 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      301 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      361 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      421 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      481 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      541 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      601 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      661 tggtgaaaca aataaaaaag tgagagtcgg accaaacccg gctttcgctg acaacaatcc
      721 acgtaacatg ggtattgtcg agcaactaca gaacggggta acggctccca acagagcagc
      781 caggagatag cctctccctt ttctggcccc catcatctgc tggattttat ggtctggcac
      841 cttttgtctt atcaggctga caccggcact aatcactata aacagcaacg aaagctcgac
      901 ggcgagaaac acaaacatct ctgcggcatc ttgcagcatc gcaagccaac tactcatacg
      961 taacctcatt ggataaatct atatttccag aataatcgaa atgaaattca gatcaagata
     1021 tttctggtat tatcgaaata tcaaccagac agaggggtgt catatgcagt tggaagaagt
     1081 cgctaaagcg ctgaaagagc taggtcaccc gacccgtttg ttcatattta aacatcttgt
     1141 caaagccggg gaacaagggt tgccggtcgg tgagttgcaa aagcagcttg ggatacctgg
     1201 ctccacactc agccaccata tatcagcact ggtatcggta ggactggtca aacaaaaccg
     1261 tgagagtcgg acgctgatgt gtgtttcgca atacgcaatg ctggaagcga taattgaatt
     1321 cttacgcgag gagtgctgcg taaatagcaa aattgcctga tgcgctacgc tctacatatt
     1381 tcatgccata tattgaattt gcacggtttt gtaggtcgga taagacgcgt tagcgtcgca
     1441 tccgacataa acaaagcgca ctttaccaac tatgggcagt ggatatcgcc gctttatttc
     1501 aaccgcttac ccgcttcatc aaccactttc tcgccatctt ccttggagaa tgcgcctttt
     1561 tgcgcatctg gcagaatttc cagcaccact tctgaagggc ggcacaggcg agttcccagc
     1621 ggcgtcacca caatcgggcg attaatcaga atcgggtgct gaagcataaa gtcgattaac
     1681 cgatcgtcag taaatttatc ttctgcaagg cccagttcct catacggctc gacgttttta
     1741 cgcaacagcg cgcgtacgga aatccccata tcggcaatga gtttgaccag ctcatcgcgc
     1801 gttggcggtg tttccagata atagataatg gtcggttctg taccgctgtt gcggatcatc
     1861 tccagcgtat tacgcgacgt accgcaggcc gggttgtgat aaatggtaat gttgctcata
     1921 tcaatatctc attacaaagt gaaagagaga cgtagcgcca gcgcagccag cgtcacaaaa
     1981 agcaccggca gggtcatgat aatccctgta cggaagtaat atccccagct gatagtcata
     2041 ttcttctgcg aaagtacgtg cagccagagt agcgtagcca ggctaccaat tggggtaatt
     2101 ttcggtccca aatcgcagcc aatcacattg gcataaacca tcgcttcttt gataacgcca
     2161 gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta ccgtcggcat attgttcata
     2221 atagaagaga ggaaggcggt gaggaatccg gtgccgagcg tcgcggccca caggccgtta
     2281 tccgccagca cgttgagtac gccagaaaga tattccgtta atccggcatt gcgcaggcca
     2341 taaaccacca gatacatgcc gagcgagaag atgacaatct gccagggggc accgcgcagg
     2401 actttacccg tattaatcgc atgaccgcgt ttagcgacga caaataatat cagcgcgccc
     2461 acagctgcaa tggcgctcac cggaatgccg agcggttcca ggacgaaaaa tcccaccagc
     2521 agaagcagta aaacaaccca gccagttttg aacgtagcag gatctttgat cgcttctgcg
     2581 ggagatttca gcagcgccat atcgtagttc tgcggaatat ctttgcgaaa atagagatgt
     2641 aacatcacca gcgtggcaac aatcgcggcg atatccaccg gcaccatcac cgaggcgtat
     2701 tcgcgaaagc cgaggccaaa gaaatcagcg gaaacgatat tcaccaggtt ggagacaata
     2761 agcggcaggc tggcggtatc ggcaatgaat ccggccgcca tcacgaacgc cagcgtagtg
     2821 cctttactga accctaaagc cagcagcatg gcgatgacaa tcggtgtcaa aataagcgcc
     2881 gcgccatcat tggcaaacag ggcggcaacg gcagcaccga gcaggacaat ccaggtaaac
     2941 agcaagcgac cacgaccatt accccagcgt gagacgtgca gcgccgccca ttcaaaaaag
     3001 ccggactcat ccagcagcag gctgatgata atgacggcga taaacgcagc cgtcgcgttc
     3061 cagacgatat tccacaccac cggaatatca cccggatgga ccacgcccgt aactaacgcc
     3121 agtactgcgc cgagcgttgc actccagccg atgcctaaac ctttcggctg ccagataacc
     3181 aatacgatgg tcaggacaaa gatagcgcct gccagtaaca taatgcctcc cggataaaac
     3241 acatctgaaa attcatatgt gtttagctaa atttttaact gcaaatgttc ttactgtccc
     3301 cggaacagtt ttgtcgagcc aggttgcgga caatcgcctg aaccttttcc tgttcacatc
     3361 gccaggcctc atcaataatt ttcgccgccc atgctggaat atgcggtgat aagcggtaat
     3421 gaacccactt accttgcttg cggtccagca acagcccgct ttcacgcagc aatgccaggt
     3481 ggcgggagat cttgggctgc gactggtcga gagcagtgca gagatcgcag acgcataact
     3541 ctcccagttc gctgagcagt aaaacgatgc ccagacgggt ttcatcagca agaattttga
     3601 acaattggat gggtaacaga aatgacatat tgcgctcctg attgttgcag gtagtgtctc
     3661 tct
//
LOCUS       DAFAVF010000258         3656 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFAVF010000258 DAFAVF010000000
VERSION     DAFAVF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.262"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..176
                     /locus_tag="LGK42_004366"
     CDS             <1..176
                     /locus_tag="LGK42_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660525.1"
                     /translation="YSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTACK
                     KCLFLMRDIIISS"
     gene            137..256
                     /locus_tag="LGK42_004367"
     CDS             137..256
                     /locus_tag="LGK42_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK2660526.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            291..635
                     /locus_tag="LGK42_004368"
                     /pseudo
     CDS             291..635
                     /locus_tag="LGK42_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1007..1273)
                     /gene="minE"
                     /locus_tag="LGK42_004369"
     CDS             complement(1007..1273)
                     /gene="minE"
                     /locus_tag="LGK42_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBK2660527.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1277..2089)
                     /gene="minD"
                     /locus_tag="LGK42_004370"
     CDS             complement(1277..2089)
                     /gene="minD"
                     /locus_tag="LGK42_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBK2660528.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2113..2808)
                     /gene="minC"
                     /locus_tag="LGK42_004371"
     CDS             complement(2113..2808)
                     /gene="minC"
                     /locus_tag="LGK42_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBK2660529.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3328..>3656
                     /locus_tag="LGK42_004372"
     CDS             3328..>3656
                     /locus_tag="LGK42_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660530.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKI"
BASE COUNT          929 a          838 c          872 g         1017 t
ORIGIN      
        1 gatattcatc agcaacgatt acattagtca ttttattttg ccgacggcct cattgtcgaa
       61 agataagcgt acgacagtat tatctgaaaa gagtgatttt ttatccaact acacttcagc
      121 gcactgcgtg taaaaaatgc ctctttctta tgcgggatat catcatttca tcatgatgtc
      181 tttggtgagc ggtgaacaca atacacctgc gctgtctctt caggatgaat cccctggtct
      241 ggtgcctgcg ggctgatgtt gcagcagagc ttaggtcact tagacgctac tatcatttat
      301 ccaatggcat ggaatcgaaa tcagtcgata cccgcagtat atatcgtgaa ctgggtgcaa
      361 cgctgagtta caacatgcgt ctggggaacg gtatggaaat tgaaccctgc ctgaaggcgg
      421 ctgtgcgcaa agaatttgtc gatgataatc gggtgaaagt gaatagtgac ggtaattttg
      481 tcaatgattt gtcgggcaga cgtggaatat accaggcagg tattaaagcc tcattcagca
      541 gtacgttaag cgggcatttc ggggtggggt atagccatgg tgccggtgtg gagtccccgt
      601 ggaacgcggt ggctggtgtg aactggtcgt tctgaccatc aacgattaaa ctgcgcttcg
      661 gcgcagtttt cgtttacagg gggctaaaag ggaaaattct ggggcaaaaa aagcccgcca
      721 gttacggcgg gaaacctcat cctatgggag aacaatgaat aatgaaattg cggggttatc
      781 atcccccagt gtatccacac taacaatgag gttatttact caaccaggca taaacatttt
      841 gttctgtgcg tgggaacagc cttaaggtgt aaagggggag gtggaaatag caatgaggag
      901 tatcagcaag aatactcgcc gctttaccac aacgtggatg agagggatga aaaactcaag
      961 gcagaaatga ctctgccttg aagataaatg cgtttttaca gcgggcttat ttcagctctt
     1021 ctgcttccgg taaggtcacg ttcagctcaa gaatagaaat atcgccatct ttttgctcaa
     1081 gctgtacggt taccatctca ggatcaattt gtacgtattt acaaatgacc tcaagaatat
     1141 ctttacgcaa ctgcggcaga taatgcggtt ctgcatcgct gcgacggcgt tcagcaacaa
     1201 taatctgcag ccgttctttt gcaatgttgg ctgtgttttt cttccgcgag agaaagaaat
     1261 cgagtaatgc cataacttat cctccgaaca agcgtttgag gaagcctttc ttctcttctt
     1321 caatgaagcg gaaaggacgt tcttctccca acagacgttc tacggtatct gcgtaggctt
     1381 tacccgcatc ggcgttaatg tcgagaatga ccggttcacc ctggttagag gcgcgcaata
     1441 ctgattgatc ctctgggatc acgccgacga gtttgatgcg caggatctcc agcacatctt
     1501 ccatgctcag catgtcacct ctgcttacgc ggcctgggtt atagcgcgtt aacagcaggt
     1561 gctctttaat aggctcttcg ccattttctg cgcggcgtga tttcgacgcc agaatgccta
     1621 aaatacggtc agagtcgcgt actgaggaga cttccgggtt ggtggtaata atggcttcgt
     1681 ctgcaaaata gagtgccatt aacgcaccgg tttcaatccc tgccggggag tcacaaacga
     1741 taaattcaaa atccatcgct ttcagatcat caagaacttt ggcgacccct tcacgggtga
     1801 gggcatcttt atcgcgtgtt tgcgatgccg gaagaatata gagattttca gtacgcttat
     1861 ctttaattaa cgcctgattt agcgttgcat cgccctgaat gacgttgacg aaatcgtaaa
     1921 cgacccggcg ttcgcaaccc ataatcaggt cgagattacg caggccgata tcaaaatcta
     1981 tcacgacagt tttctttccc ttctgggcca aaccagtggc gatggccgcg ctggaggttg
     2041 tcttaccaac accccctttg cccgaagtaa caacaataat gcgtgccata gaaattcctt
     2101 gttaaaaagg gatcaattta acggttgaac ggtcaaagcg ttttcgacta actgcagtcg
     2161 cgccgctttg ccataaaatt ctgctgggat ttgatcactc agccagtatt cacctgcgat
     2221 ggacaccagt tccgccatca ggttcgtaca aaatatttgc gtttcccggt caccacttgc
     2281 ccctgcaagc gcacgaccgc gcatcatgcc atagacatga atgttcccat cggcaatcaa
     2341 ttcggcccca gcgctaacgt ggcttgtaac aatcagatca cattgtggag cataaatacg
     2401 ctgaccggaa cgcaccgggg tatctattaa acgcgttttt gtgaccggcg ttgtattttg
     2461 cgctggagcc tgtggtgcgg gagctggacg cggcgctttt tcctttcctt ccgtcaggat
     2521 aggcagcccc atcttttcaa tttcggcttt aagttgcgca tctttgcagc cacttacgcc
     2581 aataacccgc aaaccggttg ccgaaaccgc cttatgcatc gctgaccagt ttaccgggtc
     2641 ttccagtgca ctgacgttga gtacaacggg ggcatgtttt aaaaatgcgg gggcctgagc
     2701 gattttgtct tccagcgcct gatggataac cttaggttct gcctcatgca gatgaaccac
     2761 agataaagtg aagctactgc ctttaagctc gattggcgtg tttgacatcc tggccttact
     2821 caattagcta ttaatcatcg ccagcgcgcg atgatgttcc gaagactata aggcatgtta
     2881 tagtctggat tatattgagg caagtcaccc tcccatttat tcagagtaaa agtctattct
     2941 gtgataaatg gcgctgattc atagcttaaa aaataccctt gtcaatcaac ccattgccgt
     3001 cgtacttttg attgttctta tttacgcttc ctttttccgc accctaatcg tgacagcatc
     3061 tccagacctg tttaaatatc cagtattgag gcaagtgtat tttttcacca cgccaggctt
     3121 tcttaacttt ccattttttg cggcatcatt aaagaacaga tacccgttat cttaatatcc
     3181 gccacagaat tgctctatca tagaaaccgc agaagacgtc tgatacttct gtcaggtcat
     3241 cgtaactcgt ccaggtcagc ggttttgatt acggattgat gatgaaatga gcataagtgt
     3301 tgtacggttg gattatcagg atgtgctatg aatatgatgc gtatttttta tatcggattg
     3361 tcaggtgtgg ggatgatgtt ctcatcaatg gcgtctggac atgacgctgg cggacttcaa
     3421 tctccggcgt gcggcgttgt ttgcgacccg tatatttgtg tgaactcaga tggtatttcc
     3481 ccagagttaa caaggaaata tctcggcgaa aaagccgctg aaaacttaca atcattacaa
     3541 ggctacgatc ccagcgaatt tacattcgct aacggtgtat tttgcgatgt taaagaaaaa
     3601 ttatgtcgtg atgatcgtta ttttggtgtg gatggtaagc ggagtggaaa aatcaa
//
LOCUS       DAFAVF010000259         3624 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFAVF010000259 DAFAVF010000000
VERSION     DAFAVF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.263"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..329)
                     /gene="hiuH"
                     /locus_tag="LGK42_004373"
     CDS             complement(<1..329)
                     /gene="hiuH"
                     /locus_tag="LGK42_004373"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBK2660531.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPV"
     gene            462..1133
                     /gene="hprR"
                     /locus_tag="LGK42_004374"
     CDS             462..1133
                     /gene="hprR"
                     /locus_tag="LGK42_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBK2660532.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1133..2491
                     /gene="hprS"
                     /locus_tag="LGK42_004375"
     CDS             1133..2491
                     /gene="hprS"
                     /locus_tag="LGK42_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBK2660533.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2599..3327)
                     /gene="hchA"
                     /locus_tag="LGK42_004376"
                     /pseudo
     CDS             complement(2599..3327)
                     /gene="hchA"
                     /locus_tag="LGK42_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
     gene            complement(3343..>3624)
                     /locus_tag="LGK42_004377"
     CDS             complement(3343..>3624)
                     /locus_tag="LGK42_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBK2660534.1"
                     /translation="FGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVIS
                     KRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1014 a          745 c          844 g         1021 t
ORIGIN      
        1 tcaacgggga tctcagggaa gaaactttca agattttgtt tcttgaaata gtccccggtt
       61 ttaaatacga cacggtaatc gcccgtagtt gcagtttgct cgggccacag tgccttaatt
      121 cgtccatcct tatctgtttt ggcggtatta agttgtaacc agccgttgtc cgctttcttt
      181 tcaagagtga ctgtcacgtc ggcagcgggt tttcctgttt gctggttcaa aatgtgcaca
      241 ctaagaatgt tttgttgtgc agcatagacc aaagaaggta atgaaaatgc agccgttact
      301 acggagagta ctaaataacg ctttaacatg tttatatcct tgtcatgtga atgagttttg
      361 aggatagcga aaaagcgtcc acggtaacct tacagcagca ttacaatttt gtaatgaacc
      421 aggctgtttc tataacatat gatttatggc atattatttt catgaagatt ttacttattg
      481 aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca
      541 ttgatgccgt ttctgatggc agagatgggc tttatcttgc gcttaaggat gattatgcat
      601 tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta caaacgttaa
      661 gaacagcaaa gcaaactcct gttatttgcc ttactgcaag agattctgtc gatgacagag
      721 tcagagggct ggacagtggg gcaaatgatt atctggtaaa acctttttca ttttctgagt
      781 tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca acattagaaa
      841 tcagtggctt aagaatggac tctgttagtc aaagtgtgag cagggacaat atcagtatta
      901 cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct ggcgagatta
      961 tccccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt gataccaata
     1021 cggtggacgt cgccattcgc aggctccgcg caaaagttga tgatcctttt cctgaaaagc
     1081 taatcgccac aatccggggg atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag
     1141 attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg ttgctggcgc
     1201 tggaattgta tggactctct ataatggcct ggcaagtgag ttgaaatggc gcgatgatac
     1261 aacactcatt aaccggacag cgcagatcaa gcagttgtta attgatgggg taaatccaga
     1321 tacgttacct gtgtacttta accggatgat ggatgttagt caggatatct tgatcattca
     1381 tggtgatagc atcaacaaaa ttgttaaccg gacgaatgtc agtgatgaca tgttaaataa
     1441 catacctgct actgagacaa tcagcgcagc tggcatttac agaagcatta ttaatgatac
     1501 agagatagat gctttacgaa ttaatattga tgaagtttcg ccatcattaa cggttactgt
     1561 ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatta atagcattat
     1621 aatttgcatt gtcgccattg tactttgctc agtattaagt ccgctgttaa tcagaacggg
     1681 attacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata acgatagccg
     1741 ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
     1801 taaaatgcat catgctttag tcaaagattt tgagcgtcta agtcaatttg ctgacgatct
     1861 cgctcatgaa cttagaacgc ccattaatgc attactgggt cagaatcagg ttacgctcag
     1921 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg aagagctgga
     1981 aaatatttcg cggttaacag agaacatact gtttcttgcc cgggcagata aaaacaatgt
     2041 tttggtgaaa ctggactcac tttttctcaa taaggaagtc gaaaatttgt tggattatct
     2101 tgaatacctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc agcaaatctt
     2161 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaatg ccattagata
     2221 ttcaccagaa aaatcgcgca ttcatataac cagttttctt gataccaacg gctatcttaa
     2281 tattgatatc gccagtcctg gaacgaaaat taatgagcct gaaaaactct tccgtagatt
     2341 ttggcgggga gataattcgc gtcattccga aggtcaggga cttggtcttt ctttagtcaa
     2401 agcgattgcc gaattgcatg ggggaagtgc tacgtatcac tattttaata agcataatgt
     2461 gttccgaatt acgttaccgc aaagaaatta acatagggct tcagtacgcc ataaatcaac
     2521 catgaaaaaa cgcagccacg tcgtgagtac taacgcggct gcgattaatt atgcgcttac
     2581 attcaaacgt aacagggatt aacccgcgta agctgccagc atttcctgcg ccgccagttt
     2641 acccaacgca ttcgctgcga aaggactgtc gccggtgaga agtttacggt ccttatgtac
     2701 tcgcccggtg atgtcgtcat taatgatgtt catgcccatt ttcttcagct cttcgccgaa
     2761 gtaccaggtg agatgacctg gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc
     2821 tgggaatgcg caaatggaat aaccattcag tgggttatcg ccgtggcgaa gcgccagaaa
     2881 agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgtaa
     2941 agcggcagct acgtcctggc tttcaggtaa accaataagt gcgccatgac caccaggaac
     3001 aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttttt
     3061 cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt catctttgtg
     3121 cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc
     3181 gaaacctgca gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt
     3241 cgagaacagt tttccgttat cagtcggcaa ataacgttcg tccgcggcga tcaccagaat
     3301 tttatgttta ccgcgatacg gttttggggt aatgactcca acttactgat agtgttttat
     3361 gttcagataa tgcccgatga ccttgtcatg cagctccacc gattttgaga acgacagtga
     3421 cttccgtccc agccttgcca gatgttgtct cagattcaga ttatgtcgct caatgcgctg
     3481 agtgtaacgc ttgctgataa cgtgcagctt tcccttcagg cgtgattcat acagcggcca
     3541 gccatccgtc atccatacca cgacctcaaa ggccgacagc aggctcagaa gacgctccag
     3601 tgtggccaga gtgcgttcac cgaa
//
LOCUS       DAFAVF010000260         3613 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFAVF010000260 DAFAVF010000000
VERSION     DAFAVF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.264"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(110..1429)
                     /locus_tag="LGK42_004378"
     CDS             complement(110..1429)
                     /locus_tag="LGK42_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBK2660535.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLNHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1560..3095)
                     /gene="gadC"
                     /locus_tag="LGK42_004379"
     CDS             complement(1560..3095)
                     /gene="gadC"
                     /locus_tag="LGK42_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBK2660536.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3251..>3613)
                     /locus_tag="LGK42_004380"
     CDS             complement(3251..>3613)
                     /locus_tag="LGK42_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBK2660537.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
BASE COUNT          945 a          891 c          908 g          869 t
ORIGIN      
        1 attgcgtggt taatttgact ttagtaagca tgattagtag tgggatagtt caagagggta
       61 acaagccggt gggtaaagca ccggcttgtt acaaagtaag aatgggagtt taactgcccc
      121 aacgactttg cagatagctg accgcttgtt gagtctgcgg tttattcaga tagtcctcac
      181 ggaacaagat ggtgccgcta atttcgggta ccgcatcgtt aagatcgagc tgctttttca
      241 gttcgggtac gccgccgtta atcatccagt ctggctctat ctttgaaggt tcacccactt
      301 tatagaaggc gataccgata tacaggcggg tcctggtcgg tttaacgaca tccgcccacc
      361 attttgccaa cacgtcataa cgcgcggcac tccgtgagaa cggccagtaa atttggggag
      421 caatataatc cagcaatcct tgttccaccc atcgacgggt gtcagcgtag gattcgtcat
      481 aggctgccgc acctcgggta tcggaaccga gcggatcgtg tgatcggtta cgccacacgc
      541 ctgccgggct aacgccaaat tcgactcccg gcttaatgct tttaatggtg tgcgatactt
      601 ttgcaattaa ctgctgagta ttgttgcgcc gccagtctgc ttttgacgca aatgcgcctc
      661 cgtatttgcg gtacgtttcg ttatcattta gccgtgaacc cggtgactcc gtatagaaat
      721 agtcgtcaaa ctgcacgcca tctaccggat agcgggaaac cacttctgcg actattgatg
      781 tgatccagtc ctgaacctca gggatgcccg ggtcgaggac aaagcgatcg ccagacgttc
      841 tgatccagtc acggtgttgc acatagacgc tcgccggttg ttgagacaga gtgctattca
      901 gttccctgat agtaccgggc ttcgtattaa ccgatacgcg ataggggtta aaccaggcgt
      961 gtactttcat cccacgcttg tgggcttcgt cgagcataaa ttgcagcgga tcgtaacccg
     1021 gattttcacc aatcttaccg gtcataagat cggaccacgg caaaattttc gatggccaca
     1081 gggcggtacc gtccggcttg acctggaaaa aaaccgtgtt tataccgaga cgttgcagat
     1141 gattcagttt gtcgatcatc gcctgttgtt gtacacgggc ccggctggtg gggttactaa
     1201 tgttaaccga ggaaaccggt ggccagtcga ggcgagaaac cgtggccagc cagatgccac
     1261 gcatcggttg tgacgattgt tgcgtcgtgg ctggtggctt tgaacctgct ggtggtgtca
     1321 ccatggactc tggaggcgtg cttttacaac tacacagtaa aagtgccagt gcaactagta
     1381 tcgctggtct tctaatcgtt aatttcttgt ttcgggagca gatatccata tgtgctggtt
     1441 tccggtaaac agatgtgcgc tcattctcgt acttatcccg gttaagtcaa tacgacagca
     1501 agcacgaaaa agggagcgat gacttatcgc tcccttgtct tataaccatt cagacatggt
     1561 tagtgtttct tgtcattcat cacaatatag tgtggtgaac gtgcacgcgg gtgcaggaag
     1621 aagtgacctt ttggtgcgtt ctgactgttg attggctcca gagtgacgcc ggtattcgct
     1681 ttgcctttac gatcatgaac agcatagaga ataaagggca gggcaagtac caccaggaaa
     1741 ctaacaacca gtaattcaac atacatatcg gtagaatcac cctggatgtt atccggcggc
     1801 aggaaggaaa caataaacgc cataattgaa gtcagcagac cgacaattgc cacgaccagt
     1861 ttcacccctt taccaccagg gatattaaat gtgcgtttta agtcaggatg tttaagaacc
     1921 aacacaatgt agccaataaa cagcatgaaa taagcacaca gataaatcac caccgtcagc
     1981 gccagagcga tcaggaagga catgttgtta ccgccaccgg tattggtgag gatgatcaac
     2041 gcgatagaag taatcaccag ctgcgaaatg accagcgtta ccggtacgcc atttttgttc
     2101 attttagcga atgccgctgg cagcaggttt ttctgcgctg tgacatacat cccgcgagaa
     2161 ggaccaacaa tccaggaggc gatttccgcc aaaacaccca gcaacagcag tgcggagatc
     2221 acgcgaaccg tccactcaat ttccggtgcc acatgggaca tcagaacggt aaaggtttgc
     2281 attacacctg cggagaggtt gatttcatta cccggaatga ccatcgcaat agacaaaccg
     2341 ccaacagagc ttaagcagat tgccgccacc atcagcagta acatagccag tggatagtcg
     2401 cgcccggggt tgctcatttc attgacgtga gttgcggaag cttctacgcc catataactc
     2461 aaaatgaagg caacaaatac aaccagtgtg cccactttag agaagtcagg gaagaaggtc
     2521 ttcgaatcca tttcgatagc aacgggggca ccggagtgca gataaatagc cgctaatgcg
     2581 atcaaaataa atgcaggtaa caggataccg gcgaagaagc caactttagc aattcgcgcc
     2641 gtgtatttcg tgccaccaaa ctgcgttaat gccagcgccc aaagaatgat gagtgctgca
     2701 atagttttgg taatggggtc ttcattcagc gctggccatt tcaggatgta ggagagtgcc
     2761 cctaacacga aatagagcat cggaataaaa ccaatggcga tttgcagata gccaaatgag
     2821 atcgctgcaa atccccatct cggccccaga gtatttgata cccaggcgaa gacaccacct
     2881 tcttcccagc cgtcgacggt ggccatttcc gcagcacaaa gtcccacggg aataaaccat
     2941 aaaatcccgc ctaatagcag gaagaagact aatgaaaagc ccgatgttgc gaaggtaggg
     3001 tattcataaa cagccattac catcgatgcc gttatggcaa agaatccgag taatgtgagc
     3061 tgcttagctt tacctgtctg tactgatgta gccatattat ccccctaaaa cggtattcct
     3121 gtcggaaccg cacctgtttt gttctgaagc gtattcagaa caatattttc cgttgctaat
     3181 gccagtgaac agactttgga aattgtcccg aaacgggttc gtttcgggac accgttaccg
     3241 ttaaacgtta tcaggtatgt ttaaagctgt tctgctgggc aataccctgc agtttcgggt
     3301 gatcgctgag atatttcagg gaggctttgt agtcttccag caacagttca gcaaagtcca
     3361 tttcgaagcc gcgacgacac ataatgcgca tcaccacgat gtcggtggct tcaccgccga
     3421 gagtgaaggc cggaacctgc cagccgcgca gacgcagacg ttcagagagg tcgtacaggg
     3481 tgtatcccgg atcttcacca tctttcagtt tgaagcaaac cgccgggatg ccttcgtccg
     3541 ggcgacccgt acagatgaac tcatacggcc ccagtttggc gatttcatcc gccagataag
     3601 cggcaacctg gta
//
LOCUS       DAFAVF010000261         3572 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFAVF010000261 DAFAVF010000000
VERSION     DAFAVF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.265"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(17..736)
                     /gene="dcuR"
                     /locus_tag="LGK42_004381"
     CDS             complement(17..736)
                     /gene="dcuR"
                     /locus_tag="LGK42_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBK2660538.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(733..2364)
                     /gene="dcuS"
                     /locus_tag="LGK42_004382"
     CDS             complement(733..2364)
                     /gene="dcuS"
                     /locus_tag="LGK42_004382"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBK2660539.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2545..2775
                     /locus_tag="LGK42_004383"
     CDS             2545..2775
                     /locus_tag="LGK42_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBK2660540.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2787..3059
                     /locus_tag="LGK42_004384"
     CDS             2787..3059
                     /locus_tag="LGK42_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK2660541.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3286..>3572
                     /gene="ghoS"
                     /locus_tag="LGK42_004385"
     CDS             3286..>3572
                     /gene="ghoS"
                     /locus_tag="LGK42_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBK2660542.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQ"
BASE COUNT          920 a          812 c          879 g          961 t
ORIGIN      
        1 gataaccagc gatccgttat tggcaatatt gtttcagcag tgagtagtgt tctgcctgaa
       61 tacggtaacg gtaaaccgga cgccccgtga cgccataatg gatactggtg aacaagatgt
      121 ggcagttgac cagccagatg aggtattttc ggcaggaaac acgcgagatg ttaacctcgt
      181 tagccagctc gtcggttgaa aactcatagt cctgatgcgc gtcaatccac tggcacagcg
      241 tgcgtaacgt ctgcggcgtt aagccttttg gcaagcgacg aggatcctgt tcgttggagc
      301 tgctgccgtg gattagctga tcaagctcgg cctggtcata atactgatgt ttttccagcg
      361 ccattttctt ttgccgccag ccggtgagcg cctcttcaaa gcgggaagcc tggaagggtt
      421 tgatcaggta atccacgaca ccgtaatgca gcgaatcttt aatggttgcc gcatcggctg
      481 cggaggagat gacaatcaca tcacttttgc aacgcgcgtt atgcaggaca ggcagtaaat
      541 cgagcccgtt ctctttttgc atatagatat cgagcaatat caggtcgata ggcgtatcgc
      601 tattgaagat aatctctttg gctttctcca gcgtcgaggc tgttccacag cattgaaagc
      661 ctgggatttg tgctacgtat cggcgattca gctccgcgac cattgcgtcg tcatcgataa
      721 ttaatacatt gatcatctgt tcgacctctc cccgtcccag ggtatctgga caaaaaattg
      781 tgtgaaaatc ccgggttccg attccacggc gatgctgccg ccgagatttt ctacctgttg
      841 tttgacaagt gctaaaccga cgcctcgctc gcttcctttt gtcgagacac ctttgtcaaa
      901 aatgtgatcg attttgtcgg gtgcgatccc cggtccatca tcgttaactt cgcagtgcag
      961 ccagccgtga cggtagtgca atgttacgct aatttcgcca ccgggttccg gccctaatgc
     1021 ttccagcgcg ttttctatca gatttcccaa tgtggtaatc agcgtcgcga cctggtcctc
     1081 actgccgctg tctggcagct ggctttcact gtttaaaatc agcgtatggc ctaaatcggt
     1141 cgcgcggtta atcttgctga ttaaaaaacc agcgataacc ggagatttga tcttacccag
     1201 cagagagcca atctcttcct gatagttatt ggctgttttg agaatgtaat cttccaactg
     1261 cttataactc ttcagatgca ataatccgag aatcacatgc aatttattca taaattcgtg
     1321 ggatcgttca cgaagtgcat cagcatagtt gaccagaccg tcgagtcgct gcatcagttt
     1381 acgaacttca gttttgtccc tgaaggttga aatggcaccg atgataacgc cattactgcg
     1441 caccggaacg gtgttgatca gtaatagccg gtctttaatc gtaatctctt cgtcgcggcg
     1501 cggcgtaccg tcgcgtaaca cttccgagac atctaccacc tgtgaccatg agtggcttag
     1561 cgtcgacagt ttctcatcgt cctgcgactt acggtaattc agcaattctt gtgcggcatc
     1621 gttgatcagc gtgacctcgc cgcgatcgtc cacggcaacg acgccttctt tgatagactg
     1681 caacatggcc tggcgttgct caaacagcgt ggagatttcg tagggttcca ggccgaaaag
     1741 gatttttttc agtaccttaa ccagaatgca ggtgccaatc agtccgacca gcatgccaaa
     1801 taataccgac cagataatgc tccagcgact gtcattgatt tgttgggtca cgcggcttaa
     1861 ctcaaggccg atcgccacca cgccaatttg tttatgattt tcatcgtaga tgggggtaaa
     1921 tacgcgtaaa gcctgtgcca gaaaaccgcg attgatagcg acattttctt tgccattcag
     1981 cgctttaagg atgtcatcac ctttaaatgg ctgaccaata cgctgggctt caggatgcga
     2041 gtagcgaaga ctttgcatat cggtaacgac aataaacagc agatcgttgc gtttgcgcac
     2101 ggcttccgcg atggcctgga ttccgctctc ctgcggtttt ttctgcaagc cctgacggat
     2161 ttccggcgag tcggcgaggg tacgcgccat tgccagtgcc ttgttggcca gcccgtctcg
     2221 cgtcatatca ctgatttgcg agaagtaaat cagatgcacc accaatagca ccgagaacag
     2281 taccgcgctg accattaaga tcactgtggt actcaatttc atcggacgtt tgcgtaacat
     2341 gcggtagggc aatgaatgtc tcatcagctt ccttgtgtga caaatttctt aagcattatc
     2401 tctgatgagg cgggtaattc aaagggagta agaatgattg gctatatagg ggaagagact
     2461 ctggcaacgg aaactgccag tgctgtatga agattccagg gctatgctta tagcgataat
     2521 catactgatg agagagggaa agtcatggat caggcgctac tggacgaggg ttatcgctgt
     2581 tataccggcg aaaagatcga tgtctatttc aacactgcga tatgtcagca ttctggcaat
     2641 tgcgtacgtg gcaacggcaa gttatttaat ctcaaacgaa agccgtggat catgccggat
     2701 gaagtcgacg ttgccactgt ggtgaaagtg attgatacgt gcccgagtgg tgcgctgaaa
     2761 taccgtcata aataagcgag ggtaaaatgg aaatacgcga aggccacaat aaattttaca
     2821 ttaatgacga acagggcaag caaatcgctg aaattgtctt tgtgccgacc ggagagaatt
     2881 tagcgattat cgaacatacc gatgtcgatg aaagcctgaa agggcagggg attggtaaac
     2941 agctggttgc gaaagtcgtg gaaaaaatgc gtcgggaaaa acgaaaaatt atcccactat
     3001 gcccatttgc gaaacacgaa tttgataaaa cgcgggagta tgatgatatt cgcagttgat
     3061 gggagagtac agagtcacga tatttttcat gctctccgcg gagtgatgca ggagagcatc
     3121 tgaagggtag ggggatgcac aaagaatggg cagagagcgc gtttttttgt cccaagtcat
     3181 cccctttact gagcaaaaaa aagaatatct cctatatgag aatcatcaat cggggttaat
     3241 aatttttgcg tccccagagc attcaatatt gataggagtc atattatgga aggtaaaaac
     3301 aagttcaata cttatgttgt ttcttttgat tatccatcat cttattcctc agtgttctta
     3361 aggttaagat cattgatgta tgatatgaat ttctcttcta tcgtggctga tgaatatggg
     3421 ataccacgac aattgaatga aaattctttc gcaataacga catcgttagc cgcaagtgaa
     3481 atcgaagatt taatcaggct taaatgctta gacttaccgg atattgattt tgacctcaac
     3541 attatgacag ttgatgacta tttccgtcag tt
//
LOCUS       DAFAVF010000262         3509 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFAVF010000262 DAFAVF010000000
VERSION     DAFAVF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.266"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..851
                     /locus_tag="LGK42_004386"
     CDS             <1..851
                     /locus_tag="LGK42_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBK2660543.1"
                     /translation="YCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIGRDAQ
                     ALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIRAGMI
                     GEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSP
                     NKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHL
                     IELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLCIDNP
                     GRLLTA"
     gene            complement(891..1183)
                     /locus_tag="LGK42_004387"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="LGK42_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2882)
                     /locus_tag="LGK42_004388"
                     /pseudo
     CDS             complement(1263..2882)
                     /locus_tag="LGK42_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3166..3509)
                     /locus_tag="LGK42_004389"
                     /pseudo
     CDS             complement(3166..3509)
                     /locus_tag="LGK42_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          864 a          954 c          823 g          868 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtggg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgca
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatagtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgatccagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct gggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caagtccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc agtactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggacc cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg atagaactcg agtaccagtt gctgcgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cggaaagcat aattttcttc acccctgatt
     1621 attctccagg gcatcaacca gcggcttacc cgggacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggatccagcc tttacgccat ttggcaacag catccctgac gatggtttca
     1741 ctgcctgtaa cccccctggc caccatttcc cgccatatcc ggccggagtt ataattacca
     1801 cattctcgtt gctcttttaa ccactccctc catgggtcca gaagccctcg ctttggaggc
     1861 ctagttgaca tttcagggaa tgttcctgaa cccacccagc gactgactgt cactcgcgac
     1921 aggcctgtaa tacgggatat ttccctgaaa ctacatccct tgttatgcag ggccataacc
     1981 tctgtccaac gctgatgccg ttttttgcgg atgtgctgtt taaggcgttc cagacgacgg
     2041 agcgatgcta caggcacaga tatttctggc tcaggtgatt tcttcagtga taactctctg
     2101 acaacaagac gtatcagagg catatgtctg tacatcattc gttcaggctc atcaccaata
     2161 ttttttagca ggtgccatcg atcggccacc tgtctggcct gaggtgcacc ttcacgtgct
     2221 gctgtcgcat agactccact gcgatcacgc gagacaacct gtatttccgg atattttctg
     2281 aaccaggtcg ccagcgtacg ctgatcacgg ccgggaagca ggacgagggg acggtgagta
     2341 tcaagattga ctattaacat accgcagcag tggccccgat gccacgccca ctcatcaata
     2401 ccgacatgag gcgcgcctga ctgtttcgtc tccggggtat tgataaccct gcgaagaaga
     2461 gtatcagcac tgcaacggag acccgctgcc gttgcagccc gttttccagc ctcacctcca
     2521 gctattaatc ccagttgacg ttgtaaattt tgtaacgcct gtgaagactg ctggtgtgaa
     2581 ccggcgaagg gagcaagcga ctcggcaaaa attttacgtg aacaagccgg gttacggcag
     2641 taccagtggc ggacggaaaa tacaagccat agcgtctgcc ctgagcaggg tagatgctgt
     2701 atccggcgcc gacgacaact atgaacagag tcgctacgct taaagcattc aggacattgt
     2761 gctgttttgc gttttccgtg gagatggagg gttataccgt cagagctaat aatttgtcga
     2821 ctgcatcgcc atccgcaagg taactgtaga agtgtcttaa gagagtgcat tgcgttgccc
     2881 ataacttaaa acagcttaga tccttgttcg ccagaaagtt tcatccattt tcttccaccc
     2941 aggttgcgga agaaccactt ttcatccgtc gttgacagat gtgaagtatg cgggagaaaa
     3001 agtgaatatc taactgtaat aactcagacc ggtacgagta ctcggaacgt ctacaatcat
     3061 tcagggcaaa gtgctaaaat ttctctatcc cgggatgccc ctaccaacgc agcactcccg
     3121 gagatatcat tacagctctg caactgattt ccgcgactga accgctcagt tcctatcctt
     3181 ccagttgcct tcctgcagcc cccctccctg ctgtataccc cggtaccaca acgcgggcag
     3241 cacttttctc cttcataatc ttcgtcacac atcacacaaa accactttct caccttcacc
     3301 gtataatccg gaaccgtcat accctgcaga tgcaaacgac gggcctcttt ctctgccggg
     3361 tcaagccagg tgcatcgcag caggattttc tcagcgacag tcatctggtc atgttcaaac
     3421 cacggtggtt gtccgtcatt gccccgctga agtgttaacg ggcatccaca ggacacacag
     3481 cacaaaacgt cctggcggtt gtgcttcgc
//
LOCUS       DAFAVF010000263         3483 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFAVF010000263 DAFAVF010000000
VERSION     DAFAVF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.267"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            98..1249
                     /gene="yiaY"
                     /locus_tag="LGK42_004390"
     CDS             98..1249
                     /gene="yiaY"
                     /locus_tag="LGK42_004390"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBK2660544.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1414..2952
                     /gene="aldB"
                     /locus_tag="LGK42_004391"
     CDS             1414..2952
                     /gene="aldB"
                     /locus_tag="LGK42_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBK2660545.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3171..3314)
                     /locus_tag="LGK42_004392"
     CDS             complement(3171..3314)
                     /locus_tag="LGK42_004392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660546.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
BASE COUNT          871 a          840 c          919 g          853 t
ORIGIN      
        1 attagattaa ttttgataat taataataat gatcatgttt tcgaatgaaa atcctcagta
       61 agctgcccac ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat
      121 tccttctgtg aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga
      181 ttatggattt acccgtacct taattgtcac tgacagtatg ttaacgaaat taggtatggc
      241 gggcgatgtg caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac
      301 ccaacctaac cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagagaataa
      361 ttgcgatagc gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc
      421 gctggtggca gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa
      481 accgcagctg ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg
      541 tttctgcatc atcactgacg aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt
      601 cactccgctg ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac
      661 cgccgcaacg ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc
      721 cacgccgatc actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaatctgcc
      781 gttagccgtt gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt
      841 cctcgccggt atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca
      901 gctgggcggt ttctacaacc tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt
      961 tcaggtattc aacagcaaag tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg
     1021 cgtgaacgtg acaggtaaaa acgatgcgga aggtgctgaa gcctgcatta acgccatccg
     1081 tgaactggcg aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga
     1141 agatttcgcg gttctggcga ctaatgccct gaaagatgcc tgtggtttta ctaacccgat
     1201 ccaggcaact cacgaagaaa ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc
     1261 acaacggctg gcaaattgtc agccgctttt tcaactatct ctgtaaccct tgcccgtaaa
     1321 ttcgtgatag ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac
     1381 ccttgttata cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag
     1441 attaagcccg gcgagtatgg tttccccctc aagttaaaag cccgctatga caactttatt
     1501 ggcggcgaat gggtagcccc tgccgacggc gagtattacc agaatctgac gccggtgacc
     1561 gggcagctgc tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat
     1621 gctgcgcaca aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt
     1681 ctgtttaaga ttgccgatcg aatggaacaa aaccttgagc tgttagcgac ggctgaaacc
     1741 tgggataacg gcaaacccat tcgcgaaacc agtgctgccg acgtgccgct ggcgattgac
     1801 cattttcgct atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat
     1861 agcgaaaccg tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg
     1921 tggaacttcc cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac
     1981 tgtgtggtgc tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatc
     2041 gtcggtgatt tactgccgcc gggcgtggtg aacgtggtca acggcgcagg tggggaaatt
     2101 ggcgaatatc tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa
     2161 gtgggccaac aaattatgca atacgccacg caaaacatta ttccggtgac gctggagctg
     2221 ggcggcaaat cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc
     2281 gataaagcgc tggaaggctt tgcactgttt gcctttaacc agggtgaagt ttgcacctgt
     2341 ccgagtcgtg ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc
     2401 cgtgtcgaaa gcattcgcag cggtaaccca ctcgacagcg tgacgcaaat gggcgcgcag
     2461 gtttctcacg ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc
     2521 gctgacgtgc tcacaggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac
     2581 tacctcgaac cgacgattct gtttggtcag aacaatatgc gggtgttcca ggaggagatt
     2641 tttggcccgg tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg
     2701 aacgatacgc aatatggcct gggcgcgggc gtctggagcc gcaacggtaa tctggcctat
     2761 aagatggggc gcggcataca ggctgggcgc gtgtggacca actgttatca cgcttacccg
     2821 gcacatgcgg cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg
     2881 atgctggagc attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg
     2941 gggctgttct gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta
     3001 cgcgtcttat catgcctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat
     3061 ccggcataaa caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac
     3121 ccagactacg ccaacagaag tgcgtttctg gggcgtgttt tgtcaggagt ttaatctcgc
     3181 gtttcgatgg tgagcgggcg aaccaggccg taaatcacca ttggtgatcc gaggccaatc
     3241 agcaataaac ggtagataat tgaaggttta atacgcttaa tctgtactgt ggatgtttta
     3301 ctggagttat tcattagttt ggccaatagt tccactattt caatatgata aacctcgtcc
     3361 tgttgaaaaa ccagacagca catttaagaa agtatataaa aaacaatatg tcgttatata
     3421 aataaggaaa ttgcactgca tcatggcatt tcataatatg cgttaattgt tcttttttat
     3481 att
//
LOCUS       DAFAVF010000264         3483 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFAVF010000264 DAFAVF010000000
VERSION     DAFAVF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.268"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(59..163)
                     /locus_tag="LGK42_004393"
                     /pseudo
     CDS             complement(59..163)
                     /locus_tag="LGK42_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(165..488)
                     /locus_tag="LGK42_004394"
                     /pseudo
     CDS             complement(165..488)
                     /locus_tag="LGK42_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1231..2208
                     /locus_tag="LGK42_004395"
     CDS             1231..2208
                     /locus_tag="LGK42_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK2660547.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2487..3227)
                     /locus_tag="LGK42_004396"
     CDS             complement(2487..3227)
                     /locus_tag="LGK42_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2660548.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(3348..3473)
                     /locus_tag="LGK42_004397"
     CDS             complement(3348..3473)
                     /locus_tag="LGK42_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2660549.1"
                     /translation="MSWHYPNSPKNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT          833 a          882 c          879 g          889 t
ORIGIN      
        1 gtcaacgcca cgatgtttga ccgttatttg ccattttcat gcccgctaaa tactttcgtt
       61 actgatgctg tccgctttgc accatttcat catcactgat tggcgttgtg tttttggtca
      121 gcactccagc tttacgtttc tcttttaacc ggtaactctc tccttgttct ttttgacgga
      181 cgcattttac gtttgggctg gatgtagtaa cgcagcatgg aacgtccgcc cgtataaccc
      241 attgccttaa tctccgcgaa gataacctcg ctattccaga cattctctgc caggcgcatg
      301 tcgatgtaat ccataaacgg tttcagctta accattttgt ggcgggtctt tctggctggc
      361 ggttcagggt atttgaggta gcgtctgacc gtccgttcag agcaaccaat ctgagccgca
      421 atatcgacaa tgtacgcgcc ctgctggcgc atttgcttta tcatgtaaaa gtcctctctg
      481 ctcagcatgt tgatgtcctt tctggtgtga gaaccttaag gaaacaacat gttgggtgga
      541 gcggacaatc caaatggtga attaccgtct tatatcactg gcgctgacac ctgtagtttg
      601 tatacacaat ccctggcatt gaaacacaca tatgaagtgt acttcggata tgaccgtacg
      661 ctcaggaagt acggcagatt ccgtcaaaaa ttacctgtaa agacagacat cagaaaaaac
      721 agtgctcgcc gcagaccggt gaccgggcga agcccgcgaa ccggcgagga agcgcaatgg
      781 atctgtagtt acagtgagca ggtacgccct gaagccttca taatcggtag tgtatgctgt
      841 ttttctggaa tgatttatcc acatatccag aggcccgatc cagtaaacag atccatgaat
      901 gatcaacaaa ggatccatta aagatcccca tatcgctgtc aaccttgtca ctcatgggcc
      961 gggaccacgt tcacataagc ggtggcatgt tatggataaa ctgtaacagg ttcatgataa
     1021 gctgtattca gtaatgcata tactgaagta agttaatgac ataaactatg gtcagtacgc
     1081 cagactcagt tgttaaatac agactgcagg tttttcttca gtcagttgac ggggttctga
     1141 cacacgattt tctgtttatt cttttactgt ccacaggcag gaggctttct ggaaaacgaa
     1201 aattcagaca tcaaaaaact gttcggcgag gtggataagt cgtccggtga gctggtgaca
     1261 ctgacaccaa acaataacaa caccgtacaa cctgtggcgc tgatgcgtct gggcgttttt
     1321 gtaccgaccc ttaaatcact gaagaacagt aaaaaaaata cactgtcacg tactgatgcc
     1381 acggaagagc tgacacgtct ttccctggcc cgtgctgagg gattcgataa ggttgagatc
     1441 accggccccc gcctggatat ggataacgat ttcaagacct gggtggggat cattcattcc
     1501 tttgcccgcc ataacgtgat tggtgacaaa gttgaactgc cttttgttga gtttgcaaaa
     1561 ctgtgtggta taccttcaag ccagtcatcc cgcaggctgc gtgagcgcat cagcccttcc
     1621 ctgaagcgca ttgccggtac cgtgatctcg ttttcccgta ccgatgagaa gcacacccgg
     1681 gaatacatca cccatctggt acagtcagcc tactacgata ctgagcggga tattgttcag
     1741 ttacaggctg atccccgcct gtttgaactg taccagtttg acagaaaagt ccttctccag
     1801 cttaaggcga ttaatgccct gaagcgacgg gagtccgccc aggcactcta cacctttata
     1861 gagagcctgc cccgggatcc ggcaccgata tcgctggcgc ggctgcgtgc acgcctcaat
     1921 ctgaagtctc ctgtattttc ccagaaccag acggtcagac gggcaatgga gcagctgcgc
     1981 gagattggat atcttgatta cacggagatc cagcgggggc ggacaaaact cttctgcatt
     2041 cactaccggc gtccccggtt aaaagcaccg aatgatgaga gtaaggaaaa tccgttgcca
     2101 ccttcacctg cggaaaaagt cagtccggag atggcggaga agcttgccct gcttgaaaaa
     2161 ctgggcatca cgctggatga cctggaaaaa ctcttcaaat cccgctgaac ataaactgta
     2221 gtcagtgaag agtgctcctt tactgactac agcttatatt atcaggtgca gtgagtggtc
     2281 tgctcactgc agtttatatt cagtttcatg cagtgctgcc tgtggctgag ctgccatctg
     2341 cctgtccctt acgtgagtca ccccgtaacc tgatgctgag gcattgctcc cttcataaaa
     2401 catggtttac tcactacagc ttatctacct gctccagctt atgttatgtc tgttctgctg
     2461 accactgctt atataaggaa gtgccgttac ttcaggggag gcagtgtacg caggatctcc
     2521 gcagcatccc gcccgtcacc tgtgaaaggc actgccagcg tggctgccat atccagtgca
     2581 aacactctgg tataaacctc catagaacgt ggatccctgt gaccagccag tgcctggatg
     2641 actttccggg gctggcggtg atagagcatg tgcatgatat agctgtgccg gaaggtgtgt
     2701 ggtgtgaccg gaatcgaaaa gtgtactccg tcagcttcgg cccgtctgac agcctgcttc
     2761 agccagttgc gcatggtttc gtcggtcacg gcccataatg gttcacgacg acggggccgg
     2821 gtggtgatca tccagctttc catctgcctg acatagctta tatccgtcag cggaaccagg
     2881 cggacttcat cttttggcgg gcgtccgcgt cgcgcacgca ctttttcgga caggatccgc
     2941 acaaacggtc ttactccatc caggtcaaat gattccggtg tcagcatccg ggcttcgcca
     3001 atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg atccggcata
     3061 tagaaaagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc ccctgtggaa
     3121 gtggccattc ttctcaggga gagcgctgtt gcaaaatcca cccccggcgc aatgggtaac
     3181 agggagacgc gttctggtga attctgcagg ggaatgacat tgttcatgag ttatttttca
     3241 cgtagcacac gcactgttta tggatacagg tattctgacc tgaacgcccg gatccttcca
     3301 cgacaataaa tcgcagcgcg cggatccttt tgtttgctta tcacgcatta ttgcgggtaa
     3361 aaccaaatac gtaaaggtac gttttgcagc agggatcccg tattaagcca gtcgtggcgc
     3421 gggctgaggg ctggtttgcc gtttttggga gaattggggt aatgccagga caggcagaaa
     3481 cgg
//
LOCUS       DAFAVF010000265         3446 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFAVF010000265 DAFAVF010000000
VERSION     DAFAVF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.269"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            47..238
                     /gene="ydfD"
                     /locus_tag="LGK42_004398"
     CDS             47..238
                     /gene="ydfD"
                     /locus_tag="LGK42_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HBK2660550.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            331..2802
                     /locus_tag="LGK42_004399"
     CDS             331..2802
                     /locus_tag="LGK42_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK2660551.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGLQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            2875..3126
                     /locus_tag="LGK42_004400"
     CDS             2875..3126
                     /locus_tag="LGK42_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBK2660552.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            3161..>3446
                     /locus_tag="LGK42_004401"
     CDS             3161..>3446
                     /locus_tag="LGK42_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBK2660553.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKDVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETE"
BASE COUNT         1055 a          768 c          855 g          768 t
ORIGIN      
        1 tttcaatgct ggctcgttta cgtctgatgc caaaaggatg tgcacaatga attcagcatt
       61 tgtgcttgtt ctgacagttt ttcttgtttc cggagagcca gttgatattg cagtcagtgt
      121 gcacaggaca atgcaggagt gtatgactgc agcaaccgaa cagaaaattc ccggtaactg
      181 ttacccggtc gataaagtta ttcaccagga taatatcgaa atcccggcag gtctttaaaa
      241 cagttccgta ataaatatcc ggtttcattc ttatatgcca gcaatggcag ggatttgttc
      301 atccttaaat ctgtcatgag gttaaaacaa atgagtaaag tctttatttg cgccgctatt
      361 cctgacgaac tggcaacaag ggaagaaggc gctgtggctg tagccacagc cattgaagct
      421 ggcgacgaac gccgtgctcg agcaaaattt cactggcaat tcctggaaca ttatccggct
      481 gctcaggact gcgcttataa atttattgtc tgcgaggata aacctggcat accccgccct
      541 gccctcgatt catgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct
      601 tcttttgtcc cggttgagac tgaatccgat ccgatgaacg tcacttttga caagctggcc
      661 cctgaagtac agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat
      721 atggttatta gcgcacagga attgttgcag gaagacatgg caacattcga cggacatatc
      781 gttgaagcgt tgatgaaaat gccagaagtt aacgccatgt atccggagct taagttgcac
      841 gccattgggt gggttaagca taaatgtatt cctggtgcta aatggcccga aattcaggca
      901 gagatgcgca tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg
      961 tctgttgttg atctcgcccg cgccagagcc aatcaacagt acactgaaaa ttcaacagga
     1021 aaaatcagcc cggtcattgc tgccattcat cgcgaataca agcagacatg gaaaacactg
     1081 gatgacgaac tggcctacgc tctctggcct ggtgatgtgg atgccggaaa cattgacggc
     1141 agcatccatc gctgggcaaa aaaagaagtt atcgacaacg accgcgaaga ctggaagcgt
     1201 atctcggcat caatgcgcaa acagcctgat gcccttcgct acgaccgcca aactattttt
     1261 ggccttgtcc gtgagcgtcc gatcgacatt cacaaagatc ccgtagcact gaacaaatat
     1321 atctgcgaat acctgacgac aaagggcgtg tttgagaatg aagaaacaga cctgggcact
     1381 gttgatgttc tccagtcatc agaaacacaa actgatgcag tggaaactga ggtatctgat
     1441 atcccaaaaa atgaaaccgc gccggaagct gaaccatctg tagagcgtga ggggccgttc
     1501 tatttcctct tcgcagataa ggacggagaa aaatacggtc gcgcaaacaa actctctggt
     1561 ctggataagg cactggctgc tggcgccact gaaatcacaa aagaagaata ttttgcccga
     1621 aaaaatggca catacacggg cttaccgcaa aatgtagata ccgctgaaga ttcagaacaa
     1681 ccagagccga taaaagttac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc
     1741 agccagcctg acgccgataa gttgcttgct gcatcacgtg gtgaatttgt tgaagggatt
     1801 agtgacccga atgatccgaa atgggttaag gggctccaga cccgcgattc tgtgaaccag
     1861 aaccagcatg aatcggaacg gaactaccaa aaagcggaac aaaacagtcc aaatgcgtta
     1921 caaaacgagc cagaaacgaa acagcctgaa ccagtagcgc aacaggaagt ggaaaaagtc
     1981 tgcaccgcct gcggtcagac cggcggcggc aactgccctg attgtggcgc ggtgatgggc
     2041 gacgcaacat accaagaaac attcgatgaa gagtatcagg ttgaagttca ggaagatgat
     2101 ccggaggaaa tggaaggcgc tgaacatcca cacaaggaga acactggcgg caatcagcat
     2161 cacaatagcg ataatgaaac tggcgagacg gcagatcacc caattaaggt gaacggtcat
     2221 cacgaaatca catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg
     2281 ggaaaaaatc ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc
     2341 ggagatatgg gaccggaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt
     2401 gatcgggaca ccattaaatg gtggcttaag caatcacgcg aagcgcaatc tgccattatg
     2461 accgatgaaa tcccgttaga tgatgcactg ttacaattgc gagaatttat cgacgaaaac
     2521 tccggtgaat tttttgttca ggtttgggga aatggagcca acttcgacaa cacgattttg
     2581 cgccgttcat acgaacggca ggggatcccc tgcccgtggc gttactacaa cgatcgcgat
     2641 gtacgcacaa tcgttgagct ggggaaagcc atagacttcg atgccagaac ggctattcca
     2701 ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa atacgtttca
     2761 gttatctggc aaaaactgat cccgagtcag gctgattctt aatgttcaac tgtcgccggt
     2821 tgtgactggt attctgcaac cggcgctcgt ctgatgtaag agataaagaa atcgatgagc
     2881 gaagtaatca tgattgtctc tcccggcaaa tgggtatccg aagagcagtt aattgcgctg
     2941 aaaggaataa aaaaaggtac gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg
     3001 gaatataagc atgtcgctca tgacggtatg ccatgggata acagtccatg cttttacaac
     3061 ctggaagaaa ttgatcgctg gattgagcgc caggcatcag cgagaccaag acgtcatctt
     3121 acttgactaa aagtcacact aactaatgag agaagttgaa atgaaatatc cgacaggcgt
     3181 ggaaaaccat ggagggaaat tacgtatctg gtttgtttat aaagacgtaa gagtcaggga
     3241 aaatctgggg gttcctgaca cagcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc
     3301 tgtttgttac gcaataaaaa ctggtgtttt cgactatgca aaacagtttc cctcctcacg
     3361 caatctggaa aaatttggtg aggcccgaca agatttaacc ataaaagaac tggctgaaaa
     3421 atttctggca ctgaaagaaa ctgaag
//
LOCUS       DAFAVF010000266         3424 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFAVF010000266 DAFAVF010000000
VERSION     DAFAVF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.270"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..407)
                     /locus_tag="LGK42_004402"
     CDS             complement(<1..407)
                     /locus_tag="LGK42_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660554.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKL"
     gene            complement(502..708)
                     /locus_tag="LGK42_004403"
     CDS             complement(502..708)
                     /locus_tag="LGK42_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBK2660555.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1032..1457
                     /locus_tag="LGK42_004404"
     CDS             1032..1457
                     /locus_tag="LGK42_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBK2660556.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1454..1804
                     /gene="tnpB"
                     /locus_tag="LGK42_004405"
     CDS             1454..1804
                     /gene="tnpB"
                     /locus_tag="LGK42_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK2660557.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            1835..>3424
                     /locus_tag="LGK42_004406"
     CDS             1835..>3424
                     /locus_tag="LGK42_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HBK2660558.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPW"
BASE COUNT          866 a          845 c          975 g          738 t
ORIGIN      
        1 agtttgaggc gcaactccgt gccaccaaac tgacggaaga ggctgatcag tttatttgcc
       61 agcgaacgct gcctcagacg ttcacattca gacaggagaa tgccttccag catcggatga
      121 tgaaccagca gatgttcact ggtgcataaa ttcactatca gatcatcatc caggggcggt
      181 gtggcccgtt tgtcagggcc ataaatcagc cggtgcaggc tctggggtac gatggcaatc
      241 tccggttttt caccattgaa tgacacccaa ccgggcgtca gattatggaa gtagtagata
      301 ttcggatgac acacgcccca ggatacggcg tgccgggtga agccggtcag ccatttctgg
      361 tagctttcgt ggagccattc gtagcgggtc tgatactgtt gtttcatttt catattcctc
      421 acggggatat aacaggcagc agaatgccgc cggcaggccg gagattacgt ctgctgcggg
      481 tgaacggata acggtcagaa attacggacg ggactgtgca atacgcgcct gcagccaggc
      541 ttccacttca cttttcagcc agcgggagct gcgctccatt ttgatggggg caggaaagcc
      601 cccatctttg atgagtttgt aaaaccactt atcggttaag ccggtaagtt gagtgataaa
      661 cgccatgtcg accatctggt catccatcag cgaaacaggg gtagccatga gaatgttcct
      721 ccggatgttg ttaaaccagc cggtgtggtc atgaaaacag cgcgccacct tcactggcat
      781 aacaaccggg gagaaacgta aaaaataatc gggaatgtgg ctgtccggag ataaacgggg
      841 ggattgcgat ggcagagccg tagcggaatc ccgggtggtt gttgcgaaaa gatgcttccg
      901 cacgcacgcg cgataaagga gggaggactt tcgtgagaca aggggagtaa gcgtaaactg
      961 accgccgtat gtagccatca gacgagaatt ggtaacttag acgcccatct gatatagacg
     1021 gacatctaag tatggaatta caggactggc gaaaagaacc tcgtaaaaac tattcgaatg
     1081 aattcaaact tcgtatggtg gaactggcat cacaacctgg agcttgtgtt gcacagattg
     1141 cacgtgaaaa tggcgtcaat gataatgtta ttttcaaatg gctcaggctc tggcagaacg
     1201 aagggcgtgt ttcgcggcgt cttccggtaa cgacctcttc tgacactggc gttgaattat
     1261 tacctgtaga aataacgccg gatgagcaga aagaacctgt ggcggccatt gcgccgtctt
     1321 tatccacttc cactcagacc agagtcagtg ccagttcctg caaggtggaa ttccgtcacg
     1381 gtaacatgac gctggaaaat ccatcgccag agctgctcac agtgttgatc cgtgaactga
     1441 ccgggagggg aagatgatct cactcccatc aggtacccgt atctggctcg ttgccggcgt
     1501 taccgatatg cgtaaatcct tcaacggact gggagaacag gtacaacatg tgctgaatga
     1561 taatcccttc tccggtcacc tgtttatctt ccgtggccga cggggtgaca ccgtcaaaat
     1621 tctttgggct gatgctgatg gtctgtgcct gttcaccaaa cgcctggagg aaggccagtt
     1681 tatctggcct gcggtacgtg acggcaaggt atccattacc cgctcgcaac tggcaatgct
     1741 cctcgataag ctggactggc gtcagccaaa aacatccagc cgtaactcac tgacaatgtt
     1801 gtaaaaaact cctgaccgca ttataaaaac ggtcatgagt cagaaatacc tcattcgcat
     1861 cgcagagctg gaaaggttgc tctctgagca ggctgaagcc ctccgtcaga aagaccagca
     1921 actgagtctg gttgaagaga cggaagcctt cctgcgctct gcactgacac gtgccgaaga
     1981 aaagatcgaa gaagatgaac gggaaataga acatctgcgg gctcagatag aaaaactgcg
     2041 ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt gaagttgaac tggctgaggc
     2101 tctgctgaaa caacgtgaac aggacagcga tcgttacagt gggcgggaag acgatcctca
     2161 ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg ttaccggcac accttccccg
     2221 tgaaatacac cgcctggagc cagaagaaag ctgttgcccg gagtgtggcg gtgagctgga
     2281 ttatctgggg gaagtcagcg ctgaacagct ggaactggtg agcagtgccc tgaaagtgat
     2341 ccgcacagaa cgggtaaaaa aagcctgtac aaaatgtgac tgtattgttg aagcaccggc
     2401 gccgtcccgc ccgatagagc gtggtatcgc gggccccgga ttacttgccc gcgtgttaac
     2461 gggaaaatac tgcgaacatc tgccactgta tcgtcagagt gaaatctttg cccgccaggg
     2521 tgtcgaactg agccgggcct tactctccaa ctgggttgac gcgtgctgcc agttaatgac
     2581 accggtgaat gatgccctgt accgttatgt aatgaacacc cgcaaggttc acactgatga
     2641 cacaccggta aaggtactgg caccgggtca gaaaaaggcg aaaacagggc gtatctggac
     2701 gtatgtccgg gatgatcgca atgtgggttc gtcatctcct ccagcggtct ggttcgcgta
     2761 ctcgccgaac cggcagggga aacacccgga gcaacacctc cgccccttcc gaggtatcct
     2821 gcaggcggat gcgttcacag gttacgacag gttgttcagt gcagaacgtg aaggtggtgc
     2881 actgacagaa gttgcgtgct gggcccatgc ccggcgaaaa atccacgatg tatacatcag
     2941 cagcaaaagt gcgacggcag aagaagccct gaagcgaatc agtgaactgt acgccatcga
     3001 ggatgaaata cggggattac cggagtcaga gcgtcttgcc gtcaggcagc agcgaagcaa
     3061 agtgttactg acgtcgctgc atgaatggat ggtggagaag aatggtacgc tgtcgaaaaa
     3121 atccagactg ggcgaagcgt tcagctatgt actgaatcag tgggatgccc tctgttatta
     3181 cagtgatgac ggtctggcgg aggcggataa taatgctgcg gaaagagcgc ttcgtgcagt
     3241 ctgtctcgga aagaaaaact ttatgttctt tggcagcgat cacggcggcg agcgtggagc
     3301 actgttgtac gggctgatcg gcacctgccg tctgaacggt atcgatccgg aagcgtatct
     3361 gcgccatatc ctgagcgtac tgccggaatg gccttccaac cgagttgacg aactcctgcc
     3421 atgg
//
LOCUS       DAFAVF010000267         3401 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFAVF010000267 DAFAVF010000000
VERSION     DAFAVF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.271"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            35..2458
                     /gene="pgaA"
                     /locus_tag="LGK42_004407"
     CDS             35..2458
                     /gene="pgaA"
                     /locus_tag="LGK42_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HBK2660559.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2467..>3401
                     /gene="pgaB"
                     /locus_tag="LGK42_004408"
     CDS             2467..>3401
                     /gene="pgaB"
                     /locus_tag="LGK42_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBK2660560.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKS"
BASE COUNT          943 a          784 c          816 g          858 t
ORIGIN      
        1 agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca gaaaaaggtg
       61 cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag cgagtcccgc
      121 ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc gcaagggtaa
      181 tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata accaaattgc
      241 tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta ccgtttacaa
      301 ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg tcgcttatcg
      361 taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct ctctggagcc
      421 gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgctg gtcactatga
      481 tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag ccaatttact
      541 cgcagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac gggcgatgac
      601 agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg tgcaggcatt
      661 acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag atattcgcgc
      721 tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg aaagtgaacg
      781 ttatgccatt gccgatcgcg ccctcgccca atacgctgca ttagaaattc tgtggcacga
      841 taacccagac cgtactgccc agtaccagcg tattcaggtt gatcatcttg gcgcgttatt
      901 aactcgcgat cgttataaag acgttatttc tcactatcag cgattaaaaa agacggggca
      961 aattattccg ccctgggggc aatattgggt tgcatcggct tatctcaaag atcatcagcc
     1021 gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca ttgccccgga
     1081 tttatccgat gaagaacttg cggatctctt ttacagccac ctggagagtg aaaattatcc
     1141 gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc ttcggttaat
     1201 gggcacgcct acgagcatcc cgaatgatac ctggttacag gggcattcgt ttctctcaac
     1261 cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag agcttgctta
     1321 taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac aagcccgcgg
     1381 ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc cacgtaatat
     1441 taatctggag gttgaacaag cctggacagc attaacgtta caagaatggc agcaggcagc
     1501 tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg tacgattaaa
     1561 acgtgcggtt gatgtacata atcttgcaga gcttcgtatc gctggctcaa caggaattga
     1621 tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg tttattcacc
     1681 accgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg gacaatttag
     1741 cgaaggaaaa gggattgttc gcgactggct tgcgggtgtt gagtggcggt cacgtaatat
     1801 ctggctcgag gcagagtacg ctgaacgcgt tttcaatcat gagcataaac ccggcgcgcg
     1861 cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac tggaacgcct
     1921 ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca gtgctcaggc
     1981 ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg ctttcactga
     2041 tttttccgac agtaaccagc gtcatgaagt ctcacttgag ggtcaggaac gcatctggtc
     2101 ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa atacagaaca
     2161 cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat ttgaggcaag
     2221 ccatttgtta tggcgaagct atgaaaatag ctgggagcaa atattcagcg caggtgttgg
     2281 tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct acgggcaacg
     2341 cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa aacgacctta
     2401 tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca gattttaagg
     2461 ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat aattatgctc
     2521 accgcgtgca ttagccagtc aagaacatca tttataccgc cacaggatcg cgaatcttta
     2581 ctcgccgagc aaccgtggcc gcataatggt tttgtagcga tttcatggca taacgttgaa
     2641 gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg tgaacaattt
     2701 gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg tgaagcacat
     2761 cgaggaggaa aaccgctacc ggaaaaagct gtagtgctga cttttgatga cggctaccag
     2821 agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc tgtatgggcc
     2881 cccgtcggca gttgggtcga tacgccagcg gataaacaag taaaatttgg cgatgagttg
     2941 gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg ttcccggctc
     3001 gttgagctcg cttctcatac atggaattct cactacggta ttcaggctaa tgccaccggc
     3061 agcttattgc ctgtatatgt aaatcgtgca tattttactg accacgcacg gtatgaaacc
     3121 gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga atacctgcgt
     3181 acaaaggttg aggtaaatcc acacgttttt gtttggcctt atggcgaagc gaatggcata
     3241 gcgatagagg aattaaaaaa actcggttat gacatgttct tcacccttga atcaggtttg
     3301 gcaaatgcgt cgcaattgga ttccattccg cgggtattaa tcgccaataa tccctcatta
     3361 aaagagtttg cccagcaaat tattaccgta caggaaaaat c
//
LOCUS       DAFAVF010000268         3328 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFAVF010000268 DAFAVF010000000
VERSION     DAFAVF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.272"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            20..193
                     /locus_tag="LGK42_004409"
     CDS             20..193
                     /locus_tag="LGK42_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660561.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            195..539
                     /locus_tag="LGK42_004410"
     CDS             195..539
                     /locus_tag="LGK42_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBK2660562.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            543..878
                     /locus_tag="LGK42_004411"
     CDS             543..878
                     /locus_tag="LGK42_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBK2660563.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(935..3256)
                     /locus_tag="LGK42_004412"
     CDS             complement(935..3256)
                     /locus_tag="LGK42_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2660564.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVNITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGAGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGSHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          856 a          797 c          751 g          924 t
ORIGIN      
        1 ataaaaatat tgtcaatcaa tgagttattc ctcatttaag attattctca ttcacgtaaa
       61 aaacataatt cctatcatca cagccacgct cattttgaat tatctgaata atagcgagcg
      121 ttctttagtt aagcagatct taattgaaga cgaaattatt gtgtgtgcaa cttacttaat
      181 accggacatc taagttgaag aaaaaaatac ttgccttcgg gcttatctca gcattgttct
      241 gctccacgcc agcgatggcg gatatgaacc gcaccacgaa aggtgcattg ctaggcgcag
      301 gcgttggttt acttactggc aatggtgtta atggcgtact gaaaggtgca gctgttggcg
      361 ctggtgttgg tgcagtaaca gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa
      421 aagtgggtgc cgccgttggt gctgtcactg gcgttctgac cggcaatggc ctcgaagggg
      481 cgattaaagg tgccgtgatt ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag
      541 gaatgatgat gaaaaaattt gctttactgg cgggcctgtt tgttttcgca ccaatgacct
      601 gggcgcagga ctacaatatt aaaaacggtt tgccgtcaga aacctatatt acttgcgcag
      661 aagccaacga aatggcaaaa actgacagcg cacaggttgc agaaattgtt gcggtaatgg
      721 gtaatgccag cgttgccagc cgtgatttaa aaattgagca atctccggaa ctgagcgcca
      781 aagtggtaga aaaactgaat caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg
      841 caattgatga caccatgcgt gctatcggca aaaaataata tttcatatgt accgcgtcga
      901 tgaatacgac gcggtttttt agctaacggc gggatcagaa cttatatttc aggcccacca
      961 tcactgcggt gtcattgtag ccattatcac ccagctgcac gcccacattc ccccagacgc
     1021 tggccgctgg attcaggttg ccatttacgc caagtttaat ttcccccaaa ttactcgccc
     1081 catcttgttc gactttcaca ccgttcattg agacagcaaa atctttactg ttatggatcc
     1141 agttcatttc aacaaaaggt tcaaagtcgt caccgtgcga accgctgttt ttctcctgag
     1201 tacgaataaa ggtgcggaaa ccaagtcggg tctggatgtt gttatttgcg tcattctcaa
     1261 tacgtgagcc attggcttcg gtgtgcgcat tatgatcgac gcccatccag atagcctgtg
     1321 cctgtggctg gaatatccac gtgtatttcg ccgcttccag accaaaggat ttattcaggg
     1381 caaagccgta acccgcttcc agcgagacgg ttgcgccttt cgcactatag gattcatagg
     1441 ctaattcatc ccctttcacc gagtttttaa accagctata ttgcgcccag gcgtcaatgt
     1501 atgcgccttt tttactgatg tcatcggcaa accaggtggc atacagccct gcgcaatagc
     1561 ctctgacact ccctttcgaa cgataatccg acacgctgga atgagttgag ttgtagtcgc
     1621 gggcataacc agccatcact cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta
     1681 acagatcggc ccctaactgc gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc
     1741 tgtcacgcca ggcattatgc ccgccaattt gacgtagcca aagtcggctt gaacgctcct
     1801 gttcagttac aggatcgatg tagcgcattt cacccgcacg gtcgttcaga cgcataacaa
     1861 aaagagtgtt agcggctgcc aggttggcga cgtaacttcc agcttcgagg cgcaggacgg
     1921 gggtaggcgc aacagtaggc gctgaacttc ctccggagtt tgtttcaccg ccgctcgttg
     1981 cctggaagtt agtcagatac cagtttttat tggtcgcttc ggtattacct cgggttaatg
     2041 aatattcgta ggcaccggcg aaaatcctcg aatccttaat aaattcccca tttgattccc
     2101 cctcaacgct gataatctct atcccattta acgtgtaagc tccgctgcct cctgcattgg
     2161 taactttaac tcgggttgtt ccagaggtat ttccattaat aacgacttta tccgtagctg
     2221 aattatcatc acccaattca gttctgagat gcaatacgcc attattacca acgtaatttt
     2281 cagttattgt taatcttgtt ggttcgacat cccgtccatc tgcccgggaa atatagaccg
     2341 tactattatc aaccgtgagg ttgctcaccg ttgagtcagc tttaatatcc caggtactat
     2401 tatctgaaag cgacagatag gtatgagtgt tatcatctgt tgaaatatta gctgaacccg
     2461 ttatttttga attagttgca tcgacggtca aatgaccttt aatattattg ataaaaacat
     2521 ctgcagccga tgatacctca ctatccgtaa tggtcacgtt actcgtctca tttttatggt
     2581 agaggatttc atttgctgac cgtagtgagc taccagataa aatatctacc gttgcttcct
     2641 gaccatttat ggaagactcc agcacaattc cagccccctg atgcgcgact gcagatgtat
     2701 ttttcagcgt caccgtgccg ccattataaa ttttagcaat cgcggcgtca ggacccgttg
     2761 tataaatttc tccctggtta aatgaaactg tcccctggcg ggcagcgatg gcatgagcat
     2821 tgtccccatg cgtggtgata ttaacatcgg ttaatgtaac gctggcattg gtataatcaa
     2881 gcgtgtcacc agccttgacg acagtgccat tagcgtacgc ggcataactt tctgttccgt
     2941 gggttgtgat ctcccctttt gccatatcca gttttgctat ggcattattt tcattacttc
     3001 ctgtatcggt agtactttct tcatcaagag aagatacaca cgcacgcgcg cttccgggag
     3061 atgcacacgc aagaacgccg actgcgtgat tacccgcagt aacaataaat gtatcatctc
     3121 cgtcagaaaa agagggggta accgaaccgt cagagttgac tcttattatg gtgtcagtat
     3181 tcaggtttcc actcgtttga cttagcaaac caattgagta atcccccgtt gtgacaatcg
     3241 tgtttttacc ctgcaaaata acagcaccgc caaattcact tacgattgcg ctagctgtta
     3301 atgctgtgct tagaggacta ttgtcaga
//
LOCUS       DAFAVF010000269         3184 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFAVF010000269 DAFAVF010000000
VERSION     DAFAVF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.273"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="LGK42_004413"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="LGK42_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBK2660565.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="LGK42_004414"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="LGK42_004414"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBK2660566.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="LGK42_004415"
     CDS             1252..1521
                     /locus_tag="LGK42_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBK2660567.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="LGK42_004416"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="LGK42_004416"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBK2660568.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3184
                     /gene="dsbA"
                     /locus_tag="LGK42_004417"
     CDS             2601..>3184
                     /gene="dsbA"
                     /locus_tag="LGK42_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBK2660569.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFV"
BASE COUNT          793 a          769 c          782 g          840 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgtggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tttgaacgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg attgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagccgttca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttctg cttctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttca
//
LOCUS       DAFAVF010000270         3182 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFAVF010000270 DAFAVF010000000
VERSION     DAFAVF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.274"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            8..517
                     /locus_tag="LGK42_004418"
     CDS             8..517
                     /locus_tag="LGK42_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBK2660570.1"
                     /translation="MILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTVNLGEVA
                     DTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLKNTKETE
                     IGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATPGNVYA
                     QADYKIAYE"
     gene            577..3081
                     /locus_tag="LGK42_004419"
     CDS             577..3081
                     /locus_tag="LGK42_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBK2660571.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
BASE COUNT          964 a          688 c          658 g          872 t
ORIGIN      
        1 cgtagcaatg attctttcca ataacgtatt tgctgacgaa ggtcacggca ttgttaaatt
       61 taaaggtgaa gttatctctg ctccatgttc aattaaacca ggtgacgaag acttaactgt
      121 aaatctgggt gaagtagcag ataccgttct gaaaagcgac cagaaatctt tggctgagcc
      181 tttcactatt cacctgcaag actgcatgtt gagccagggt ggaaccactt attcaaaagc
      241 gaaggttact ttcaccacag cgaatactat gacgggtcag actgacctgc tgaaaaacac
      301 caaagaaact gaaatcggtg gtgccacagg cgttggcgta cgtattctgg atagccagag
      361 cggcgaagtg actttaggta ctccggtagt aattacattc aacaatacta acagctatca
      421 ggaactgaac ttcaaagctc gtatggaatc cccaagcaaa gatgctaccc cgggcaacgt
      481 ttacgctcag gctgactata agattgctta cgagtaatca cattaggccg ggttcgcccg
      541 gccacatcat taacggatta atgataagtg gaacagatgt ataaaaaatt aaaattaacc
      601 acaataagcg aattgattaa aaatatttat tgttcattat ccgttctcat cattggttgt
      661 gcgtcagctt atgccgttga attcaacaaa gatttaatcg aagccgaaga tcgtgaaaac
      721 gttaaccttt cccaatttga aactgatggc caattacccg taggtaaata ctcattaaac
      781 gctcttatta ataataaaag gacgccaatc catctggacc tgcaatgggt attaattgat
      841 aaccaaactg cagtttgtct gactccagag caattaacat tattaggctt tactgatgag
      901 attattgaag tggctcagca aaacctgatc gatggttgtt accctatcga aaaagaaaaa
      961 caaattacaa cttatctcga taaagggaaa atgcaattat ccatatctgc acctcaggca
     1021 tggttaaaat acaaagatgc aaactggacg cctcctgaac tttgggatca tggtattgct
     1081 ggggcatttc ttgactacaa tttatatgcc tctcattatg caccacatca gggcgataat
     1141 tcacaaaata taagttccta tgggcaggct ggggttaatc ttggggcctg gcgcctgcgt
     1201 actgattacc agtacgatca atcatttaac aatggcaaaa gccaggcgaa caacctggat
     1261 tttccgcgta tttatttgtt tcgcccaatc ccagcaatta atgcaaaact aactataggt
     1321 caatacgata ctgaatcctc tattttcgac tctttccatt tttctggcgt ttcgttgaaa
     1381 agcgatgaga atatgttgcc gcctgatcta cgtggttacg caccgcaaat cacgggtgtt
     1441 gcacaaacga atgcaaaggt cactgtctca cagaacaacc gtattattta tcaagaaaat
     1501 gttcctcctg gcccatttgc tattactaat ttattcaata cattacaggg gcaactggac
     1561 gtcaaggttg aagaagagga cgggcaagtc acgcaatggc aagttgcatc taatagtatt
     1621 ccttatctga cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa accgaccagc
     1681 gttggtggtg attccttaca acaacccttc ttctggactg gtgaattctc atggggttgg
     1741 ctgaacaatg tatccctgta tggtggttca gttttaacaa accgtgatta tcaatctctg
     1801 gctgccggcg ttggctttaa ccttaactca ttgggttcat tatcttttga tgtcacacga
     1861 tctgatgctc agttgcataa tcaggataaa gaaacgggtt atagctaccg cgctaactat
     1921 tcaaaacgtt ttgaatctac cggtagccag ctcactttcg ctggttaccg tttctctgat
     1981 aaaaactttg tgacaatgaa tgaatatatc aatgacacta accattacac gaattatcag
     2041 aatgaaaaag agagttatat tgtcacgttt aaccagtatc ttgaatcatt aaggttaaat
     2101 acatacgtaa gtttggctcg taatacttac tgggacgcca gcagtaatgt gaattattca
     2161 ttatcactta gccgcgattt tgatatcggg ccattaaaaa acgtctccac ttctctaaca
     2221 tttagccgaa taaactggga agaagacaac caggatcaac tgtacctaaa tatttcgatt
     2281 ccctggggaa ccagtagaac attgagctat ggtatgcaac gaaatcagga taacaagatt
     2341 tcgcatactg cttcgtggta tgactcttcc gatcgaaata attcctggag cgtttctgct
     2401 tcaggcgaca atgatgaatt caaagatatg aaagcgtcac tacgcgccag ttatcagcat
     2461 aataccgaga acggtcgtct ctatctctcc gggacatcac agcgagacag ttattattct
     2521 ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc acggagccgc tttccatgac
     2581 tatagtggta gtgctgactc gcgttttatg atcgacgcag atggtgcaga agatattccg
     2641 ttgaacaata aacgcgcggt aactaatcgg tatggcatcg gagttattcc atcagtcagc
     2701 agttacataa caacatcatt aagcgttgat acccgaaatc ttccagaaaa tgtggatatc
     2761 gaaaactcgg ttatcaccac caccttaacc gaaggtgcta ttggctacgc caaacttgat
     2821 acccgcaagg gctatcaaat catgggggtt attcgcctgg cagatggtag tcacccacca
     2881 ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt tgctgatggc
     2941 ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg ctggggtgat
     3001 aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac ggttatttta
     3061 ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa cacaaagaat
     3121 ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg tgccatcatc
     3181 cc
//
LOCUS       DAFAVF010000271         3163 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFAVF010000271 DAFAVF010000000
VERSION     DAFAVF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.275"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            30..506
                     /gene="agaB"
                     /locus_tag="LGK42_004420"
     CDS             30..506
                     /gene="agaB"
                     /locus_tag="LGK42_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBK2660572.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            545..1348
                     /gene="agaC"
                     /locus_tag="LGK42_004421"
     CDS             545..1348
                     /gene="agaC"
                     /locus_tag="LGK42_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBK2660573.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1338..2129
                     /gene="agaD"
                     /locus_tag="LGK42_004422"
     CDS             1338..2129
                     /gene="agaD"
                     /locus_tag="LGK42_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBK2660574.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2130..2885
                     /locus_tag="LGK42_004423"
     CDS             2130..2885
                     /locus_tag="LGK42_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBK2660575.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          810 a          725 c          760 g          868 t
ORIGIN      
        1 ttattcaact ttatggtgag gattacacaa tgagcagtcc aaatattctc ttaacccgta
       61 ttgataaccg tctggttcac ggtcaggttg gcgtgacctg gacatccacc atcggtgcga
      121 atctgctggt agtcgtggat gatgttgtcg ctaacgatga tattcaacag aaattaatgg
      181 gtattaccgc agaaacctac ggctttggca ttcgtttctt tactatcgaa aaaaccatta
      241 acgtcatcgg caaagctgca ccgcatcaga agatcttcct gatttgccgt acgccacaaa
      301 cggtacgtaa attggtagaa ggtggcattg acctgaaaga tatcaacgtc ggcaatatgc
      361 atttctcgga agggaaaaag caaatcagca gtaaagttta tgtcgatgac caggatctcg
      421 cggacttacg ttttattaaa caacgtggcg tgaatgtttt cattcaggac gtccctggcg
      481 atcaaaaaga acaaatccct gactaaatct aaaatcgcct taatattggt ttgaggtaat
      541 aaaaatgcat gaaataaccc tacttcaggg attatccctg gcggcgttag ttttcgttct
      601 ggggattgat ttttggctgg aagccttatt tttattccgc ccgataatcg tttgtaccct
      661 aactggcgct attctcggtg atattcagac tggcttaatt accggtggtc tgacagagtt
      721 ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg cccaacccga ttatggcggg
      781 tctgatgacc accgtcattg catggtctac gggcgttgat gccaaaacag caattggtct
      841 tggcctgccg tttagtttgt taatgcagta cgtcattctg ttcttctatt ccgctttctc
      901 attatttatg accaaagccg ataaatgcgc gaaagaggcg gatacggcag cgttttcccg
      961 gcttaactgg acaacgatgc tcatcgtcgc ttcagcgtat gcggtgattg ctttcctctg
     1021 tacttacctg gcacaagggg cgatgcaggc gctggtgaaa gcgatgcccg cctggttgac
     1081 ccacggcttt gaagtggctg gcggtattct gcctgccgtt ggttttggct tgctgctgcg
     1141 cgtaatgttc aaagcgcaat atatccctta cctgatcgcc ggtttcctgt ttgtttgcta
     1201 catccaggtc agcaacctgt tgccggttgc cgtactgggc gcaggctttg cggtgtatga
     1261 gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag cccgttgcca gtaaaaatga
     1321 agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa agatatcacc cgtctgggct
     1381 ttcgttcgtc gctgctgcaa gcgagcttta actacgaaag gatgcaggcg ggcggtttta
     1441 cctgggcgat gttgccgatc ctgaaaaaga tttataagga cgacaaaccg ggcttaagcg
     1501 cggcgatgaa agataacctc gaatttatta atacccaccc gaatctggtc ggattcctga
     1561 tggggttatt aatttcgatg gaagaaaaag gagaaaaccg cgacaccatt aaaggcctca
     1621 aagtggcact gtttggccca atcgccggga ttggcgatgc gattttctgg tttactttgt
     1681 tgccgattat ggcgggaatt tgctcatcat ttgccagcca gggaaacctg ctggggccga
     1741 ttctattttt cgccgtttac ctgcttatct ttttcctgcg cgtcggctgg acccacgtcg
     1801 gttattcagt cggcgtgaag gcgatcgata aagtgcgaga gaactcgcag atgattgctc
     1861 gctcggcaac catcctcggg atcacggtaa tcggcgggct gatcgcttcg tatgtgcata
     1921 ttaacgtggt gacatcgttt gccatcgaca gtacccacag cgtcgcgctg caacaggatt
     1981 tcttcgataa agtcttcccg aacattttac cgatggccta caccctgctg atgtattact
     2041 tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg cgtgactttt gtgctctcta
     2101 ttgtttgttc cgcattcggc attttgtaaa tggaacgagg cactgcttct ggcggtgcct
     2161 ctttacttaa ggaatttcat cctgtgcaaa cccttcagca agttgaaaac tatacggcgt
     2221 taagtgaacg agccagcgaa tatttattgg ctgtgatccg tagcaaaccg gatgccgtga
     2281 tttgcctggc gaccggagcc acgccattac tgacgtatca ttatctggta gaaaaaatcc
     2341 accagcagca ggttgatatc agccagctca ccttcgtgaa gctcgacgaa tgggtggatc
     2401 tgccattaac gatgcctggc acctgcgaaa ctttcctaca acagcatatc gtgcagccgc
     2461 tggggctgcg tgaagaccag ctcatcagct ttcgctccga agagataaat gagacagagt
     2521 gcgaacgggt aacgaacctg attgcgcgca aaggcggtct ggatttatgc gttctaggat
     2581 tggggaaaaa cggtcatctt gggctgaacg aaccgggaga aagcctacaa ccggcctgcc
     2641 atatcagtca acttgatgcc agaacacagc aacatgagat gttaaaaacc acgggtcgcc
     2701 ccgtgactcg tgggatcacc ttaggcctga aggatattct caatgcccgc gaggttttgt
     2761 tattggtgac tggcgaagga aagcaggatg cgacagaacg ttttctcacg gctaaagtct
     2821 ccaccgctat cccggcttcg tttttatggc tgcacagtaa ctttatttgt ttaattaata
     2881 cctaaccaga tgaaaatttg ctcaaaattt ttaaattaac ttattcaaca gtcacgcatt
     2941 atattaaata acatttgact gggttgaaca taacgccaat agcaaaagga gttatgttca
     3001 acaatttaat tttagatata catataactt gttctattaa taaatcacct cgtattctga
     3061 ctcggcccct ggcgcagcaa agtcaggcat ttatactctt tttaacacat agaaaattca
     3121 tatcaatata ataaaaacat agaatatttg catataaata taa
//
LOCUS       DAFAVF010000272         3156 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFAVF010000272 DAFAVF010000000
VERSION     DAFAVF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.276"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            138..677
                     /locus_tag="LGK42_004424"
     CDS             138..677
                     /locus_tag="LGK42_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK2660576.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            740..973
                     /locus_tag="LGK42_004425"
     CDS             740..973
                     /locus_tag="LGK42_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBK2660577.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1001..1198
                     /locus_tag="LGK42_004426"
                     /pseudo
     CDS             1001..1198
                     /locus_tag="LGK42_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1253..1686
                     /gene="psiB"
                     /locus_tag="LGK42_004427"
                     /pseudo
     CDS             1253..1686
                     /gene="psiB"
                     /locus_tag="LGK42_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            1683..2402
                     /locus_tag="LGK42_004428"
     CDS             1683..2402
                     /locus_tag="LGK42_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBK2660578.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYLYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(2414..2602)
                     /locus_tag="LGK42_004429"
     CDS             complement(2414..2602)
                     /locus_tag="LGK42_004429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBK2660579.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            2682..2840
                     /locus_tag="LGK42_004430"
     CDS             2682..2840
                     /locus_tag="LGK42_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBK2660580.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT          706 a          814 c          987 g          649 t
ORIGIN      
        1 tgcggcgaag gttctcaaaa aggtgatggt gaataccgga gggaacaaaa ggcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaaaagat gatgggggct gaaaacatca
      121 acagaaggag gcacatcatg gcagttcgtg gcattaacaa ggtcattctc gtgggacgcc
      181 tgggaaaaga tccggaagtc cgttacatcc ccaacggggg cgcagtggca aacctgcagg
      241 tcgccacgtc ggaaacctgg cgtgacaaac agacagggga gatgcgggag cagacagaat
      301 ggcatcgcgt ggtgctgttc ggcaagctcg cggaagtggc tggcgaatat ctgcgcaagg
      361 gcgcgcaggt ctacatcgaa ggtcagcttc gcacccgtag ctgggaggat aacggtatca
      421 cccgttacgt caccgaaatt cttgttaaga ccacgggcac catgcagatg ctgggacgtg
      481 ccgcaggtgc tcagactcag ccggaagagg ggcaacagtt cagcggtcag cctcagccgg
      541 aaccacaggc ggaggccggt acgaaaaaag gtggcgcaaa aacgaaaggc cgtggacgta
      601 aggccgcgca gccggagcct cagccgcaac cgccggaggg tgacgattac gggttttcag
      661 acgatatccc gttctgatcg ggctgactgt gataaccgcc ccgccccgtt ctgtgcgggg
      721 catcaccgga gagatgagga tgagcgaata tttcagaata cttcaggggc tgcctgacgg
      781 tccttttacc cgcaaacatg ccgaagctgt tgccgcacag taccggaacg tctttatcga
      841 ggatgaccac ggcgaacagt ttcgcctggt tgtccgtaat aacggtgcaa tggtctggcg
      901 tacctggaat tttgaggacg gtgccgggta ctggatgaac catgtcatca gggatttcgg
      961 gattcttaag taagagaggt gcccgacgcg cgaacgtccg ccgcagatta tcgcagccct
     1021 gaataaggca gggctgtccg gtgccgcacg ggacgcggag aagatgaaga aaggtgatgc
     1081 ggctgaacat gcagagcacc atatgaaaga caaccgctgg gttccaggct ggatgtgtgc
     1141 accacgccca cagacggatg ccactgaacg caccgataac ctggctgatg ccgcctgatg
     1201 aacaaccaca ccgccccgcc ggagacgggg cggcagcaag ggagataccg tgatgaaaac
     1261 tgaactgacc ctgaatgtat tacagaccat gaacacacag gaatatgaag atatccgggc
     1321 tgccggaagt gatgaacgcc gtgagctgac tcacgccgtg atgcgggagc aggatgcgcc
     1381 ggataactgg acgatgaacg gcgagtacgg cagcgagttt ggcggctttt tccccgtcca
     1441 ggtccgtttc acgcctgccc acgaacgttt tcacctggcg ttatgttcac cgggcgatgt
     1501 ctctcaggtc tgggtgctgg ttctggtgaa tgccggtggt gagccgtttg cggtggtcag
     1561 gtacagcggc ggtttgcacc ggaagctgtc agccattcac tggcactggc ggcgtcactg
     1621 gacgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga aggaagtcag
     1681 gcatgagcgc acgttcacag gcactggttc ctctcagcac agagcaacag gccgcatggc
     1741 gggcggtggc ggagaccgaa aaacgccgcc atcagggtaa cacgctggct gaatatctgt
     1801 atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatatcgctg tctgacctgc
     1861 gatttttcat gccgtccctg accgcagaag aactgcacgg caaccgtctt cagtggctgt
     1921 atgccatcga tgtgctgatt gaaacacagg gcgaggtttg cctccttccc cttccaggcg
     1981 atgcagcaga acgcttgttc ccgtcggtgc ggtttcgtgt cagggaacgc agtcgccata
     2041 aatccgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca gagcagaaag
     2101 cccgtgcgta ccaggcgctg gtcgctcagg ctgaaatcga actggcattc cattcgccgg
     2161 aaacagtcgg aagctggtat gcacgctgga gtgaccgggt ggcagagcat gacctggaaa
     2221 ccctgttctg gcagtggggc gagcgttttc cgtcactggc cggaatggaa cgctggcagt
     2281 ggcaggatat gccattctgg caggtcatcg cagaggctgg tatggcggca agggtggtcg
     2341 gtcatgctgt tcgtgagatg gagcggtgga tggtgccgaa caaactccgg gaggcagcgt
     2401 gatgcggcaa caatcacacg gatttcccgt gaacggtctg aatgagcgga ttattttcag
     2461 ggaaagtgag tgtggtcagc gtgcaggtat atgggctatg atgtgcccgg cgcttgaggc
     2521 tttctgcctc atgacgtgaa ggtggtttgt tgccgtgttg tgtggcagaa agaagatagc
     2581 cccgtagtaa gttaattttc attaaccacc acgaggcatc cctatgtcta gtccacatca
     2641 ggatagcctc ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta ccacgaagtt
     2701 cccttgtctg gtgtgtgttg atcgtgtgtc tcacactgtt gatattcact tatctgacac
     2761 gaaaatcgct gtgcgagatt cgttacagag acggacacag ggaggtggcg gctttcatgg
     2821 cttacgaatc cggtaagtag caacctggag gcgggcgcag gcccgccttt tcaggactga
     2881 tgctggtctg actactgaag cgcctttata aaggggctgc tggttcgccg gtagcccctt
     2941 tctccttgct gatgttgtac gggcatgaac aacctgactt caggaaggtc gtttcccttc
     3001 aggaacgggg atgaacgcgc gcctgcggcg cccgttcttt tcccccgcct tctctggtta
     3061 tggcctgtca gaaatcacat caccttccct gtgattattc tctttttctt cctgctctga
     3121 ttctgactac tgcagtgttg tcctgtctgc cgggcg
//
LOCUS       DAFAVF010000273         3097 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFAVF010000273 DAFAVF010000000
VERSION     DAFAVF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.277"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            261..971
                     /locus_tag="LGK42_004431"
     CDS             261..971
                     /locus_tag="LGK42_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HBK2660581.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVITDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            898..3020
                     /locus_tag="LGK42_004432"
                     /pseudo
     CDS             898..3020
                     /locus_tag="LGK42_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          933 a          730 c          711 g          723 t
ORIGIN      
        1 ctcaaaccac gctgggattt ttcctcaagt tataatcatc accccgaaaa tcattcggca
       61 tttactcatt aaatagtcac cccataggcc tgtacatgtt tactcagaaa tatacatcct
      121 tttctctgtc ataaaccctc taattaatca taaataaata cttgtgacac caatcttttt
      181 ccttaacgga acgaattgtt gtgtagaagg agataatatt atgtcaaaaa aaatatcagc
      241 cattgcttat aactcattat atgtcaacat gggtcactat aacggaagca gttgagatca
      301 ctaccaaagc cataaaacaa aaaattactc ctagcgatat ttatcgtcat gccttgagtg
      361 gcaacatcct actatcggtc tatttccagt ctcctgtgat acttaagaag atacaaactt
      421 ttaatggaaa aataaaattc aggcagtttg tgggagacct acttgataaa ctatgcatgc
      481 ttgacaggga cggttttatt tatggtcaaa atttaagact ctgtacagaa gctagatata
      541 tttgtcctgt gcaacaaatt atagataccc ccctgttacg caaactcaac cagtttcgta
      601 cgtttgttcg taatgtccgc ccgggtgatg aactggatgt cccggcacaa gttagtgaaa
      661 aaaatttaac cccgccgccg ggtaacagca gtggcaacct tgagcaacag atagccagta
      721 cttcacagct aattggatct ctgctcgccg aagatatgaa cagcgagcaa gcggcaaaca
      781 tagcacgtgg atgggcctct tctcaggctt caggggtaat aacagactgg ttaagccgct
      841 tcggtaccgc aagaatcacg ctgggcgtgg atgaagattt cagcctgaag aactcccgtg
      901 acggcaaccc tcgaaaatgg cgattcgatg caacaaacag tgaactatgt gccgaacgtc
      961 acgaatgctg aaatcacgct ggcagcctcg aaggacccgg tgattgccga caataacgat
     1021 ctcacgacac taacagcacc gtcgctgata cagagggcaa tgcaatagcc aacactgagg
     1081 taacatttac tctaccggaa gatgtgaagg cgaacttcac gctgagcgat ggcgacaaag
     1141 cggttactga tgccgatggc aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc
     1201 ataccgtgac agcatcgatg gttggcggta agagtgagca gttggtggtg aactttactg
     1261 cggatacgct cactacgcag gttaatctta acgttaccga ggacaatttc atcgccaata
     1321 acattggaat gaccaggcta caggcaacag tgactgatgg aaacggcaac ccagtagaag
     1381 gtataaaagt taatttccgt ggaacctccg tcacgctaag cagcaccagc gttgaaacgg
     1441 atgatcaggt tttcgctgaa attcttgtga caagcaccga ggtcggacta aaaacagttt
     1501 cagcctctct ggcagataaa cctactgaag tcatctcgcg attactgaat gccaaggtag
     1561 atgttaattc tgcgacgatt accagtcagg agatacctga aggtcaggtc atggtcgcac
     1621 aagacatagc agttaaagct cacgttaacg accagtttgg caacccggtg acgcatcaac
     1681 ccgcgacatt cagtgcagcg ccatcctcgc aaatgatcat cagccagaat acggtctcta
     1741 ctaatacgca gggagtagcc gaggtcacca tgacgcctga aagaaacggc tcgtatactg
     1801 taaaagcatc cctagcgaat ggagcttcac ttgagaaaca actggaggct attgatgaaa
     1861 aactgacact cacgtccagt ccgcttatcg gtgtcaatgc tcctaaaggc gctactctga
     1921 cggcaatgct aacctctgca aatggcactc cagtggaggg tcaggtcatc aactttagcg
     1981 taacgctaga aggtgcgacg ttaagtggcg gaaaagtgag aaccaactct tcaggtcagg
     2041 ctccagtcgt tctgaccagc aataaagtcg gtacatatac ggtgactgca tcgttccata
     2101 acggcgtcac aatacagaca cagacaaccg tgaaggttac tggcaaccca agcaccgcac
     2161 atgttgctag ctttatcgct gaaccgtcga ctatcgccgc caccaacagt gatttaagta
     2221 ccttaaaggc aacggttgag gatggcagtg gtaacctgat cgaaggtctc actgtgtact
     2281 tcgccttaaa aaacggctcc accacattaa cgtcattaac agcggtgacc gatcaaaacg
     2341 gaatcgcgac aacaagcgtg aaaggagcga taacggggag cgtcactgta agcacagtca
     2401 caagcgcagg tggaatgcaa acagtagata tatcgctagt ggcaggccca gcagacgcct
     2461 cgcagtccat ccttaagaac aaccagtcat cactgaaagg ggactttacc gatagtgctg
     2521 agctacatct tgttctgcac gatatatcag gcaatccgat caaagtttct gaagggatgg
     2581 aatttgtgca atcaggtaca aacgtgccct atatgaaaat tagcgcaatt gattacactc
     2641 aaaatatcaa cggtgattac aaagctactg ttacaggaga cggagagggt atcgcaacgc
     2701 tgatccccgt attgaatggt gttcatcagg cgggcctgag taccacgata gaattcatta
     2761 gcgcggaaac cagaccaatg acgggtacag tgtccgtgaa tagtgcaaac ctaccgacag
     2821 cttcattccc ttcgcaggga tttaccgggg cgtattatca gttgaataat gacaactttg
     2881 caccaggaaa aacggcggct gattattcgt tttcaagctc ggcctcctgg gtaggcgttg
     2941 atgctactgg taaagtgaca ttcaaaaatg acggtgatag caatacagta ataatcacag
     3001 cgcccccaag atccggtggt gctatttacc aaacagttcc tcctgaatcg cgttcggtgt
     3061 gactatgatc attttcggtg agtattgctt tgatcca
//
LOCUS       DAFAVF010000274         3095 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFAVF010000274 DAFAVF010000000
VERSION     DAFAVF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.278"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..468
                     /locus_tag="LGK42_004433"
     CDS             <1..468
                     /locus_tag="LGK42_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBK2660582.1"
                     /translation="YFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDR
                     IEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSA
                     YQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            550..1029
                     /locus_tag="LGK42_004434"
     CDS             550..1029
                     /locus_tag="LGK42_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBK2660583.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1045..1521
                     /locus_tag="LGK42_004435"
     CDS             1045..1521
                     /locus_tag="LGK42_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBK2660584.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1584..1805
                     /locus_tag="LGK42_004436"
     CDS             1584..1805
                     /locus_tag="LGK42_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBK2660585.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1879..2247
                     /gene="cbtA"
                     /locus_tag="LGK42_004437"
     CDS             1879..2247
                     /gene="cbtA"
                     /locus_tag="LGK42_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBK2660586.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            2336..2710
                     /gene="cbtA"
                     /locus_tag="LGK42_004438"
     CDS             2336..2710
                     /gene="cbtA"
                     /locus_tag="LGK42_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBK2660587.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2707..2901
                     /locus_tag="LGK42_004439"
     CDS             2707..2901
                     /locus_tag="LGK42_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660588.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            2914..3027
                     /locus_tag="LGK42_004440"
     CDS             2914..3027
                     /locus_tag="LGK42_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBK2660589.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
BASE COUNT          727 a          853 c          799 g          716 t
ORIGIN      
        1 tacttcaggt tcgtctgcca gaacggctgc gtctgtggtc agtctctggg ggaagtacgt
       61 gttccgcacc ggggaaatgt ggtggacaga gtcattgaag gggcttacga ggtggtgggc
      121 gtgtttgacc ggatagagga gaagcgtgat gccatgcagt cgctggtcct gccgccaccg
      181 gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga acatcagccc
      241 gtcaccaccg ccgacattct gacgccgcga cgccgggagg attacggtaa ggacctgtgg
      301 agcgcatatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg tcgcagtgca
      361 aaaggaaaac gtatccatac ccgggccatt cacagcattg ataccgacat taagctcaat
      421 cgcgcattgt gggtgatggc agaaacgatg ctggagagcc tgcgctgatg ccgtttccct
      481 gagtgaggga atcattcagc tttcgttcct gaaaaacaca catttatttg ttatttaagg
      541 agttatctca tgaaaaccgt ttctcagaat acccccacaa tttattcagc tacaacacca
      601 gagaataatc cgcctcagtt ggttgccagc ctcgtccctg atgaacagcg catcagcttc
      661 tggccgcagc attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc
      721 tggatggacc gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac
      781 aacggcgggg catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg
      841 ttcagtgcca tgaacggtaa ccgcgcagaa atgagcccgg aagccgccgg tattgccgcc
      901 tgtctgatga cgtacagtca tcatgcctgt cgtacggaga attatgccat gacggtccat
      961 tattaccggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
     1021 atcgactgaa tggagagaag cacaatgcaa caactttcct ttctgcccgg agagatgacg
     1081 cccggcgagc gcagcctcat tctgcgggct ctgaaaaccc tggaccgcca tcttcatgaa
     1141 cccggcgtgg ccttcacctc cacccgtgca gcacgggaat ggctgattct gaacatggcg
     1201 ggactggagc gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc
     1261 ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt
     1321 aaacgcgccc tgtaccacaa tgccgctgcc gtggtactgg cacacaatca cccgtccggt
     1381 gaagtcacac ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc
     1441 ctggtggata tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt
     1501 gccgaacatg gtctgcttta acccttcaca accacatcac acctgttttc acttttatct
     1561 tctgtcttca gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc
     1621 accaacaaca tccgacatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg
     1681 gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt
     1741 ttgctgaacg ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga
     1801 actgagtggg aattctgatg agcagaatta tcgccactac cggaccattc ttagccgatt
     1861 ttctgtaagg attttatcgt gtcagacaca ctccccggga caacacttcc cgacgacaat
     1921 cacgaccgcc cctggtgggg gctgccctgc accgtgacgc cctgtttcgg ggcacgtctg
     1981 gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg ccggtatcag aggcctgttc
     2041 agcgatgcag atgcgtacca cctggaccag gcctttccgc tgctgatgaa acaactggaa
     2101 ctcatgctca ccagcggtga actgaatccc cgccatcagc ataccgtcac gctgtatgca
     2161 aaagggctga cctgcaaagc cgataccctc agcagttgtg gttacgttta tctggctgtt
     2221 tatccgacgc ccgaaatgaa aaattaactc tccagaatag ccttctgcta cggcctggtg
     2281 ttttcaccac gccacttttc catttttata tctgcatatc aggaaaatct tcagtatgaa
     2341 aacattacct gtattacccg ggcaggcggc cagttctcgc ccgtctcctg ttgaaatctg
     2401 gcagatactg ctgtcccgac tgctggacca gcactatggc ctcacactga atgacacacc
     2461 ttttgccgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc
     2521 ggtgaacttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg gattcagcgc
     2581 ctgtacccgc tctcagttaa taaacagtat tgatatcctc cgggcacgcc gggcaaccgg
     2641 cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta agtatccgga
     2701 ggcgaaatga tgacactgga agccgacagc gttaacgtac aggcgctgga tatggggcac
     2761 attgtcgttg acattgatgg tgttaatatc actgaactga ttaataaggc cgctgaaaac
     2821 ggttattcac tccgcgtggt ggatgaccgt gactctaccg aaacaccggc aacttatgcc
     2881 agccctcacc agttgctgta agacaatgca gtgatacagg gtatagtgcc attaaccaca
     2941 gaaacagcac tgttgtcctg tattaagcag gcaagagaag atgtccttac cctgcgccat
     3001 ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca ggcttcattt ctcctgacgc
     3061 cagcctgaat acagctggcg ttttcattta taaac
//
LOCUS       DAFAVF010000275         3090 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFAVF010000275 DAFAVF010000000
VERSION     DAFAVF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.279"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..439
                     /locus_tag="LGK42_004441"
     CDS             <1..439
                     /locus_tag="LGK42_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660590.1"
                     /translation="SALGYITPGVVLTAPWAVVGLMQLPLSYCIQAIFKANEANDHSS
                     LTPSEVRRLVPIVKSKRLKLVLLLAFYVFSTLFVILGLIASTNDQALLFRVLKISGGL
                     LFISLYTSVFVHKIMVELQEFKALLNRRESEKKERESLLDRLK"
     gene            919..1125
                     /locus_tag="LGK42_004442"
     CDS             919..1125
                     /locus_tag="LGK42_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBK2660591.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1201..1497
                     /locus_tag="LGK42_004443"
     CDS             1201..1497
                     /locus_tag="LGK42_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBK2660592.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1503..2288
                     /gene="bet"
                     /locus_tag="LGK42_004444"
     CDS             1503..2288
                     /gene="bet"
                     /locus_tag="LGK42_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBK2660593.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2285..2965
                     /locus_tag="LGK42_004445"
     CDS             2285..2965
                     /locus_tag="LGK42_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK2660594.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2962..>3090
                     /locus_tag="LGK42_004446"
     CDS             2962..>3090
                     /locus_tag="LGK42_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HBK2660595.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAE"
BASE COUNT          818 a          756 c          767 g          749 t
ORIGIN      
        1 gtcagcattg ggctatatta caccgggtgt tgtcctaaca gctccgtggg ctgtggttgg
       61 actcatgcaa ttgccattat catattgtat acaagccatt tttaaggcaa atgaggccaa
      121 tgaccactcg tctttaaccc ctagtgaggt taggcgatta gtgccaatag tcaaatccaa
      181 gagactgaaa ttggttttat tattagcttt ctatgtattt tcaactttgt ttgtaatctt
      241 ggggttaata gcaagcacaa atgatcaagc cctactcttc agggtcttaa aaatttcagg
      301 tggattactc tttatttctc tctataccag tgtattcgtt cataaaataa tggttgagct
      361 tcaagagttt aaagcgttat taaaccgaag agaatcagag aagaaggaaa gagagtcact
      421 ccttgataga ttaaaatgac ccccctcccc ggccgtcgtg ccgggttttc ttttgcctcc
      481 cctcatcaca caccgttcaa aaaaccacca tgcccctgct tcagttatcg ctatgcgatg
      541 caagtcacaa aattaattct ttttgctatc aaacatttaa tatcaaaaca catcagccaa
      601 tagcaataag tattgatatc accaatagca atagctatta tcatcatatc gcaacaacac
      661 aacgatacgg caaccacctg attcaccgtt gcgatgaccg cttagatccg tagcttgaat
      721 ttcagcaggc tccgggaagt gcgaggggtg aagcggacgc gtgaacgtcg gtgtgaccag
      781 ctgaaattaa ctcaacattt catacctcag tcgcttcaac gaggcggctt agttatgaca
      841 accggcggcc atccaccgcc tgaatacgcg cagaagtctt tatatgttca gcagcccagc
      901 ttacgggcag gagtttttat ggttcatcaa cattacggaa cgcagaccgt taatcgcggt
      961 gcggtcatgc caggaatgct ggtcaaacac aaagatggta cctggactgc atcagctaat
     1021 ttacgcggac ggctttatct gcatcgcggc atcgagcgca cttatacccg tgatttgctc
     1081 gtggaagttt ttctcgacgg acgcggcaac ggcctgaatc actaatcccc tttcctgttt
     1141 acctaatcag cccggcattt cgcgggcgat atattcacag ccattttcag gagttcagcc
     1201 atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg tcattaccag
     1261 cagattgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa aggtcttcca
     1321 cagcacctgt ttgaatcact ctgcatcgat catttgcaac gccacggggc cagcaaaaaa
     1381 gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc
     1441 cggtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaaac
     1501 ggatgagtac agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt
     1561 ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca
     1621 gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga
     1681 cgaaagaaat ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg
     1741 atggctggtc ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg
     1801 acaatgaatc ctgtacatgc cggatttacc gcaaggaccg taatcatccg atctgcgtta
     1861 ccgaatggat ggatgaatgc cgccgcgaac cattcaaaac tcgcgaaggc agagaaatca
     1921 cggggccgtg gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg
     1981 cccgtctggc cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg
     2041 aaaatactgc atacactgca gaacgtcagc cagaacgcga catcactccg gttaacgatg
     2101 aaaccatgca ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact
     2161 tattgccgct ctgttcccag atatttcgcc gcgacattcg tgcatcgtca gaactgacac
     2221 aggccgaagc agtgaaagct cttggattcc tgaaacaaaa agccactgag cagaaggtgg
     2281 cagcatgaca ccggacatta tcctgcagcg taccgggatc gacgtgagag ctgtcgagca
     2341 gggggatgat gcgtggcaca aattacggct cggcgtcatc acagcttcag aagttcacaa
     2401 cgtgatagca aaaccccgct ccggaaagaa atggcctgac atgaaaatgt cctacttcca
     2461 caccctgctg gctgaggttt gcaccggtgt ggctccggaa gttaatgcta aggcgctggc
     2521 ctggggaaaa cagtacgaga acgacgccag aactctgttt gaattcactt ccggcgtgaa
     2581 tgttactgaa tccccgatca tctatcgcga cgaaagtatg cgcaccgcct gctctcccga
     2641 tggtttatgc agtgacggca atggccttga gctgaaatgc ccgtttacct cccgggattt
     2701 catgaagttc cggctcggtg gtttcgaggc cataaaatcg gcttacatgg cccaggtgca
     2761 gtacagcatg tgggtgacac gaaaagatgc ctggtacttt gccaactatg acccgcgcat
     2821 gaagcgtgaa ggcctgcatt atgtcgtgat tgagcggaat gaaaagtata tggcgagttt
     2881 tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt
     2941 tgtatttggg gagcaatggc gatgacgcat cctcacgata atatccgggt aggcgcgatc
     3001 actttcgtct actccgttac aaagcgaggc tgggtatttc ccgggctttc tgttatccga
     3061 aatccgctga aagcccagcg gctggctgag
//
LOCUS       DAFAVF010000276         3079 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFAVF010000276 DAFAVF010000000
VERSION     DAFAVF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.280"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..261)
                     /locus_tag="LGK42_004447"
     CDS             complement(<1..261)
                     /locus_tag="LGK42_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBK2660596.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEE"
     gene            complement(243..665)
                     /locus_tag="LGK42_004448"
     CDS             complement(243..665)
                     /locus_tag="LGK42_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBK2660597.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(681..1421)
                     /locus_tag="LGK42_004449"
     CDS             complement(681..1421)
                     /locus_tag="LGK42_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660598.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(1429..1824)
                     /locus_tag="LGK42_004450"
     CDS             complement(1429..1824)
                     /locus_tag="LGK42_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660599.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VTSGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(1821..2399)
                     /locus_tag="LGK42_004451"
     CDS             complement(1821..2399)
                     /locus_tag="LGK42_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660600.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(2411..2764)
                     /locus_tag="LGK42_004452"
     CDS             complement(2411..2764)
                     /locus_tag="LGK42_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBK2660601.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(2776..>3079)
                     /locus_tag="LGK42_004453"
     CDS             complement(2776..>3079)
                     /locus_tag="LGK42_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBK2660602.1"
                     /translation="LRVAELEEELDDTDDAAGQDTSVSPENALTGHENEVVSAQPDTV
                     IDTAALVTVVALVTLHTDALHATRDEPVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
BASE COUNT          647 a          972 c          770 g          690 t
ORIGIN      
        1 ctcttcgtca gcctcgcgcc ggttcagcag actgaaatcc agcggatgca tctccggatc
       61 gctgaaaaac aggctgagca cggtgtacgt cagcccggaa aagtgcatat ccagcagaac
      121 atcatgaaaa taatgggtac tgtaaaagcg gtgccagtcg gcatactccg tggatgacat
      181 cccggcaagc atggcgcgcc agtcgggtcg ccccatctca cgcgccagtt tcagggcaaa
      241 actcagctca ccgtcgaaca ctttcccgca gaaacaggct ctgcaggccc ggcgtcctct
      301 gcctgttcag gagcatcatt caccacaaac tcatacatac cggacagccg gtacaccacg
      361 ttttcagcat gagaaattgc ctctgtgggc caggtggtaa gcacttcctg ctcaatctgt
      421 ttaacggctt cattcatgga aggcagcttt gtcttcttcg gatggttatg ccacagggac
      481 atcgccacca gaaacgcgcc ggttctgatg gcgtcttcca cagtaaactt ccggttgctg
      541 tctgactccg cctgttctgc ctgccgtttc atcagggcga gatgctcaat acgctgcagg
      601 gctgacagtt cagaaagcgt gacggtcaca ccgttatgtt caaatgattc ggttttcagg
      661 aacatcgctg actctccgga ttaactggcg gtgacgttga tttctgcaac cgcagcaaac
      721 tcaccattac cggatacgac cggaatgttg accttgcctg cagcaacacc tttcacggtg
      781 atggtcatac cactgaccga cacggtggct tttgttttat ccgcagacac cgcacggaag
      841 ctcttgtcgg ttgcgccatc cggctggaat gccacggtca gcgtggtgct ctgccctttc
      901 actacggaag cactggcggg tgtcaccgtc atgccggttg tcgccgtcac cgtgctgcga
      961 tcttctgcca tcgacggacg tcccacattg gtgaccttca ccgtgcgggt aatcacttcc
     1021 ttcgccgtca ccgccttacc gatactgctg acccagccac ggaacacatc gaccgtgccg
     1081 ttcgggaagc ggattttata ggcacgggta tcgccttcat taaaccacgc cagcagcgcc
     1141 tgctgcccct gctctccggg catccacgcc agcgtgaagc tggtatctcc ggcagatttc
     1201 tgcccctgcc cggtcgcagt ccagtctgca tcttcatcat cgagatagct gtcgtcataa
     1261 gactcagcgg tcagttcgcc gggcgtcagg tctttaactt ttgccagacg cgaccagtca
     1321 acgtctgaaa gcggattcgc ataagggtca ccactcccct tataaaccca cagggtggtc
     1381 ccggcccctt tcaccggcat tacaggattt ggtacaggca tagcgtcctc acatttcata
     1441 ggtaatgaca taagtcagat cggctgaact ccacaggccc gcatcatcgt cgcgccgata
     1501 gtcatagccg ctggtcacca tactggtgat caaatctgac agtgccggga tatcactcat
     1561 caccggataa atccgggact ccatccacgc atccagctct gaatccggca cctgagcagg
     1621 caggaaaact tcaatatgca gctccgcctg ccaggtatcg ctgtccagct cttcgcccgt
     1681 gtattcagcg ccggtgagat aaacggcaat tgccggaaaa tcttcctcat caaaaacagc
     1741 ggggcgacca tcaaaaagcg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc
     1801 tgctcggagt tcagtatgtt tcatcgcttt attaccatcc tcagttgatg ctgcagcgca
     1861 tagcccagct ctttcggaag acgctcacgc cgtatccgct caatattctg tttaaacgcc
     1921 gtggtcagcg gcaccgccat cgggattttc accacatcaa tggggtaacg atttttcccg
     1981 gccacacgct gcatgacatg ccagcggcca tttttcagtt gctgaataaa cgcgccggga
     2041 atacgacggt ttcccaccac aagcacgctg ccgccacctt tcagggctga acgctgcccc
     2101 tttttacgac gcctgcgtcg ggacaggaca atccgcgcgt tacccagctt tattacgggc
     2161 aaatcccccc ggttaacctt gattctggcc tgcggatttt tgaccgtggc ccttttcagc
     2221 ctggcccttt cctttaccag tttccggcgt acctttgtct cacgggcaac ctgtgacgcc
     2281 gactgcgata tcgcggatga agcaacgcgg ttaatggcca ttgcggcggc accgggcacc
     2341 gccgttctgc tgatacggct gaggttttca acggcctgct caagaccttt tatggccata
     2401 catccccctt tcagcggcga cggttaacgg caggcggtac gccccgccca agccagagat
     2461 gacagcttcc gccatcatct ggcgaaatcc ggtctatcca gaagttttcc tcaccgatgg
     2521 tcagcgtgtc gccgcgccgc agctgccgca catcatcagt ccggacaaac agggacgggc
     2581 tggagccttc aacgcgcacg ccctgtccgg catagctgat attttcaggg tcatcaaaaa
     2641 caccacgtat cacagcaccg gactgctcac cggatgtcat ggtggctgac gttcccatgt
     2701 acccgcgtat cgtttcatcg gcgcgggcaa tggcagcatc gaacaggtta tcgaaatcag
     2761 ccacagcgcc tcccgttatt gcattctggc caggccgcgc tctgtcattt cagctgccac
     2821 accggcagag acacggaacg ccgttcccgg cagcacaaat gccacaggct catcccgcgt
     2881 ggcgtgaagt gcatcagtat gcagcgtcac cagtgccacg accgtgacca gagcagccgt
     2941 atcaatcacg gtatccggct gcgctgatac cacctcattt tcatgtccgg tcagcgcatt
     3001 ttccgggctg acagatgtgt cctgaccggc agcgtcatcc gtgtcatcaa gctcctcttc
     3061 cagctctgcc acacggagc
//
LOCUS       DAFAVF010000277         3063 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFAVF010000277 DAFAVF010000000
VERSION     DAFAVF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.281"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(44..1903)
                     /gene="gss"
                     /locus_tag="LGK42_004454"
     CDS             complement(44..1903)
                     /gene="gss"
                     /locus_tag="LGK42_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBK2660603.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2108..2974
                     /gene="yghU"
                     /locus_tag="LGK42_004455"
     CDS             2108..2974
                     /gene="yghU"
                     /locus_tag="LGK42_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBK2660604.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          717 a          824 c          779 g          743 t
ORIGIN      
        1 ctgattattc ttcataatca gtacggggtt gctacaacat gtattacttt ttcaccacaa
       61 ttaacggttc aatatcactc tcttttttga tgaccagtga ttcatcaccg cgcaaacacg
      121 tcccaccgta gttgccgcca acggtgaagg tacatacctg aatgtattta ccgtccactt
      181 tcggcaaaca ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccgctggttt
      241 tgtccagcac ctcttcatga tggctgacga ggtcgatatt gctgccacag cgaccggcga
      301 tcggtttcac tgcgtaacct gttttcacca gttcatcatt aacagtgaaa tcggtatcca
      361 gcaggtaacg atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg
      421 ggatcaccgt ccacagcggc tcaaagacca gcacttccgg gcgcagcaat acgtcgataa
      481 gacgcacttc gttttgcgga tgaccggtac ggattggcac cgcagcaaac tcacggtcgc
      541 taacttcacg aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa
      601 ccagccgtcc ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccagcc
      661 cgcgcaagat acgcgtttca aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt
      721 gatagttttc ctcgatatct ttgtcctgca tgatatggac aaacggacgt gcacgactgt
      781 gtttccaggc accagccagt tcgttaatca gcccttccgc cggattgaag ccgttgcctt
      841 tatagccctg ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga catgaggcag
      901 agtcggcgtt gtattcgtaa accttcaggc cacgctcatc catgcagaaa tccatacgac
      961 cagtgatcat atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt
     1021 tcgggatgtc gaacagcgcc agcaggttgt catctttcag caccttgtcg gttgcgtgaa
     1081 gatacatcag gtgcagctcg ttggtggctt taattagctc ctgctcggca ctctcggtaa
     1141 tggtgtagta atgataagga tcctgattga tcacctgacc gttggcctgc acataggcgt
     1201 tttgcagcgg atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccaggc
     1261 gcgctccgct gattttcagc agctcgcctg caatttccgg ctgcggtaag ctgtattcag
     1321 tatcttccgt ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg tctttcaggg
     1381 tatagcagcc gttttcgacc accatctcca gctcacgcgt ccactgttgc ccttgcggca
     1441 acggggaatg aatcacgttc tgttccgcaa tacggacttt gttgccatgc aattgggtaa
     1501 tgatggcgac atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcac
     1561 ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg caatggcagg atgttgtcat
     1621 taaccacttc tcgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa
     1681 agaccacacc gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt
     1741 ggcccatata ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg
     1801 gatcgagaga actgtaatct gaagagtaga ttgctacccc acctggggcg tagcccaata
     1861 atgtcccgaa cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa
     1921 tacaacctaa gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc
     1981 ggacagaggt cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa
     2041 ttcacattct gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca
     2101 actcactatg acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc
     2161 tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct
     2221 gcccgttggc aaacacccat tgcaacttta ttcgctggga acgccgaacg gtcagaaagt
     2281 aacgattatg cttgaggagc tgctggcgct gggcgttact ggtgcagagt acgacgcctg
     2341 gctgattcgt attggcgatg gcgatcaatt ctccagcggc tttgtcgaag tgaacccaaa
     2401 ctcgaagatc ccggcgctgc gcgatcatac gcataatccg ccgatccgcg tgtttgaatc
     2461 tggttcgatc ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatttggc
     2521 aaagcgtact gaaacgatga actggctgtt ctggttacag ggcgcggcac cgttcctcgg
     2581 cggtggtttt ggtcactttt accattacgc gccgatgaaa attgagtacg ccatcaaccg
     2641 ctttaccatg gaagccaaac gtctgctcga cgtgctggat aagcaactgg cgcagcataa
     2701 gtttattgcg ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa
     2761 tgtggtgtta ggcggcgtgt atgatgccgc agagtttctt gatgcgggca gttataagca
     2821 tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg gtgaaacgtg ggcgtattgt
     2881 taaccgcacc aacggaccgc tgaatgagca gttgcatgag cgccatgacg ccagtgattt
     2941 cgagacgaat acggaagata agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca
     3001 tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac
     3061 gct
//
LOCUS       DAFAVF010000278         3060 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFAVF010000278 DAFAVF010000000
VERSION     DAFAVF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.282"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..968
                     /gene="rfaQ"
                     /locus_tag="LGK42_004456"
     CDS             <1..968
                     /gene="rfaQ"
                     /locus_tag="LGK42_004456"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBK2660605.1"
                     /translation="NYPDAKIDVLLYQDTIPILSENPEINALYGIKNKKAKASEKIAN
                     FFHLIKVLRANKYDLIVNLTDQWMVAILVRLLNARVKISQDYHHRQSAFWRKSFTHLV
                     PLQGGNVVESNLSVLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQPT
                     ARQIFKCWDNAKFSAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTALAG
                     KVTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQFW
                     AGDYREMPTRDQRDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL"
     gene            965..2089
                     /locus_tag="LGK42_004457"
     CDS             965..2089
                     /locus_tag="LGK42_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK2660606.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2082..2879
                     /gene="rfaP"
                     /locus_tag="LGK42_004458"
     CDS             2082..2879
                     /gene="rfaP"
                     /locus_tag="LGK42_004458"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBK2660607.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKLEQGLLSQAEAKATKIRERTIRKSL"
     gene            2895..>3060
                     /locus_tag="LGK42_004459"
     CDS             2895..>3060
                     /locus_tag="LGK42_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HBK2660608.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHN"
BASE COUNT          792 a          685 c          766 g          817 t
ORIGIN      
        1 aaaattaccc tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc ccgatcctgt
       61 ctgaaaatcc agagattaac gcgctctacg gcataaaaaa taaaaaagca aaagcctcag
      121 aaaaaattgc caactttttt catctcatca aggtattacg tgccaataag tatgacctta
      181 tcgtcaatct taccgatcaa tggatggttg ctatactggt tcgcttatta aatgcccgtg
      241 tgaaaatttc ccaggattat catcatcggc agtctgcttt ttggcgtaaa agtttcaccc
      301 atttggtgcc gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg ctgacaccat
      361 tgggacttga ttcgttggtg aagcagacaa ccatgagtta cccgcctgca agctggaaac
      421 gtatgcgtcg cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt atccaaccta
      481 cggcgcggca aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg attgatgcct
      541 tacatgctcg tggttatgaa gtcgttctga cgtccggccc ggataaagac gatctggcct
      601 gcgtcaatga aattgcgcag ggatgccaga cgccaccagt aacggcgctg gctggaaagg
      661 tgaccttccc ggaacttggt gcgttaatcg atcatgcgca gctgtttatt ggcgttgatt
      721 ccgcaccggc gcatattgcc gctgcagtta atacgccgct gatatcgctg tttggtgcga
      781 cagaccatat tttctggcgt ccctggtcaa ataacatgat tcaattctgg gcgggagatt
      841 accgggaaat gccaacgcgc gatcagcgtg accgaaatga gatgtatctt tcggttattc
      901 cggcggcaga tgtcattgct gctgtcgata aattactgcc ctcctccacg acaggtacgt
      961 cgttatgatc gttgcttttt gtttatataa atattttccc tttggcggtt tgcagcgtga
     1021 ttttatgcgt attgctcaga cagtcgccgc ccgaggtcat catgttcggg tttataccca
     1081 gtcgtgggaa ggcgaatgcc ctgatgtatt tgaactgatc aaagtgccgg ttaaatcgca
     1141 taccaatcac gggcgcaatg cggagtattt tgcctgggtg caaaaacatt tacgcgaaca
     1201 tcccgtcgat aaagtcgttg gattcaacaa aatgccgggg ctggacgttt attatgccgc
     1261 tgatgtttgt tatgccgaga aagtagcgca ggaaaaaggc tttttctatc gcctgacgtc
     1321 acgttatcgc cattatgccg cctttgagcg ggcaaccttc gaacagggca agccgacaca
     1381 gctgctgatg ctgacagata agcaaatcgc cgatttccag aaacattatc agactgaagc
     1441 ggagcgtttt catattctgc caccggggat ttatcctgat cgtaaatata gccagcagcc
     1501 agccaatagc cgtgaaatct tccgtaagaa gaatggaata accgaacaac aatatttatt
     1561 gttgcaggtc ggttcagact tcacgcgtaa aggtgtcgat cgttccattg aagcacttgc
     1621 ttcgttaccg gattcgctgc gccacaacac attgctatat gttgttgggc aggataaacc
     1681 gcgaaaattt gaggcactgg cagaaaaacg cggcgtgcgc agtaatgttc acttcttctc
     1741 ggggcgcaac gatgtctcgg aattaatggc ggcggcggat ttattactgc atcctgccta
     1801 ccaggaagcg gcgggaattg tgctgctgga agcgattact gcaggattac cggtactaac
     1861 aactgccgtt tgtggctatg cgcattatat tgtcgacgct aattgcggcg aggctattgc
     1921 tgagccattc cgccaggaaa cattgaatga gattttacgc aaagcgttaa cgcaatcttc
     1981 attgcgccag gcttgggcgg aaaatgcgcg acattatgct gatacacaag atttatacag
     2041 tctgccagag aaagcggcgg atatcataac gggtggtctg gatggttgaa cttaaagagc
     2101 cgtttgccac gttatggcgc ggcaaagatc cttttgagga agttaaaacc ttgcagggtg
     2161 aggtatttcg tgaactggaa actcgccgta ctctgcgctt tgaaatggcg ggcaaaagct
     2221 attttctcaa atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat ttactctcat
     2281 tgcggatgcc agtattaggc gctgaccgcg aatggaatgc gattcatcga ctgcgggatg
     2341 tcggcgttga tactatgtat ggggtggcat ttggcgaaaa aggcatgaat ccgctgacca
     2401 gaacttcatt tattattacc gaagatctga caccaaccat aagtctggaa gattactgtg
     2461 ctgactgggc gaccaaccca ccagatgttc gcgtaaagcg tatgcttatt aagcgtgtcg
     2521 cgacgatggt gcgcgatatg catgctgcgg gcattaatca ccgtgactgt tatatctgtc
     2581 atttcctgct gcacttgcct ttttccggta aggaagagga gttaaaaatt tcggtaattg
     2641 acctgcaccg ggcgcagctt cgcacgcgcg ttccacgtcg ttggcgcgat aaagatctta
     2701 ttgggcttta tttttcttcg atgaatatcg gcctgactca gcgggatatc tggcggttta
     2761 tgaaagtgta ttttgccgcc ccgcttaaag acattctcaa gctggaacaa ggactgctgt
     2821 cgcaagcaga agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa tcgttgtaat
     2881 ttccggagag aaatatgagt gcccactatt ttaatccaca agagatgatc aataagacaa
     2941 tcatcttcga tgaaaggcca gcggcgtcag tggcatcatc attccatgtt gcttatggca
     3001 ttgataaaaa ctttcttttt ggttgtggtg tttcaatcac gtcagttttg ttacataaca
//
LOCUS       DAFAVF010000279         2791 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFAVF010000279 DAFAVF010000000
VERSION     DAFAVF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.283"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            78..1352
                     /locus_tag="LGK42_004460"
     CDS             78..1352
                     /locus_tag="LGK42_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK2660609.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIMFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RNGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRYYQSKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPIND"
     gene            1327..>2791
                     /locus_tag="LGK42_004461"
     CDS             1327..>2791
                     /locus_tag="LGK42_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V synthesis protein"
                     /protein_id="HBK2660610.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVFVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIE"
BASE COUNT          809 a          554 c          653 g          775 t
ORIGIN      
        1 ataatgcaaa ttttcgattt attttccatc attaatccag ataaacaaca aactaatagt
       61 atgcaaggag gcattatttg tttcgccagg atgctttaga aaacagaaaa atgaagtggc
      121 agggacgggc aatattactt cccggaatac cactgtggtt aatcatgctg ggaagcatta
      181 tgtttattac ggcatttctg atgttcatta ttgttggtac ctatagccgc cgtgttaatg
      241 tcagtggtga ggtcacaacc tggccaagag ctgtcaatat atattcaggt gtacagggat
      301 ttgttgtcag gcagtttgtt catgaagggc agttgataaa aaaaggggat cctgtttatc
      361 tgattgacat cagtaaaagt acacgcaatg gtattgtcac tgataatcat cgccgggata
      421 tagaaaacca gctggttcgt gtggacaaca ttatttcccg tctggaagaa agtaaaaaaa
      481 taacgctaga taccctggaa aaacaacgtc tgcaatacac agatgcgttc cgtcgctcat
      541 cagacattat acagcgtgca gaggaaggga taaaaataat gaaaaataat atggagaatt
      601 acagatacta tcagtcaaaa ggactgatta ataaagatca attaactaac caagttgcat
      661 tatattatca acaacaaaac aaccttctca gtctgagcgg acaaaatgaa caaaatgccc
      721 tgcagataac cactctggag agtcagattc agactcaggc agcagatttt gataatcgta
      781 tctatcagat ggaactgcaa cgactcgaat tgcagaaaga actggttaac actgatgtgg
      841 aaggcgaaat cattatccgg gcgttgtctg acgggaaagt tgactccctg agtgtcactg
      901 tagggcaaat ggtcaatacc ggagacagcc ttctgcaggt tattcctgag aacattgaaa
      961 actattatct tattctctgg gtcccgaatg atgctgttcc ttatatttcg gctggtgaca
     1021 aagtgaatat tcgttatgaa gccttcccct cagaaaaatt tgggcagttc tctgctacgg
     1081 ttaaaactat atctaggact cctgcgtcaa cacaggaaat gttgacctat aagggagcac
     1141 ctcaaaatac gccgggtgcc tctgttccct ggtataaagt cattgcgacg ccagaaaagc
     1201 agataatcag gtatgacgaa aaatacctcc ctctggaaaa tggaatgaaa gccgaaagta
     1261 cactatttct ggaaaaaagg cgtatttacc agtggatgct ttctcctttc tatgacatga
     1321 aacacagtgc aacaggaccg atcaatgact aacaggaatt tcagacaaat tataaatctg
     1381 cttgatttgc gctggcaacg tcgtgttccg gttattcatc agacggagac cgctgaatgt
     1441 ggactggcct gcctagcaat gatatgcggt cattttggta agaatattga cctgatatat
     1501 cttcgccgga agtttaatct ctctgcccgt ggagcaaccc ttgcaggaat caatggaata
     1561 gcggagcaac tggggatggc cacccgggct ctttcactgg agttggatga acttcgagtc
     1621 ctcaaaacgc cgtgtattct ccactgggat ttcagtcact tcgtcgttct ggtcagcgta
     1681 aagcgtaacc gttatgtact gcatgatccg gccaggggca taagatatat cagccgggag
     1741 gaaatgagcc gatattttac aggcgttgca cttgaggtct ggcccggaag tgaattccag
     1801 tcggaaaccc tgcagacccg cataagtctt cgttcactga ttaacagtat ttacggtatt
     1861 aaaagaacgc tggcgaaaat tttctgtctg tcagttgtaa ttgaagcaat caatctgcta
     1921 atgccggtgg ggacacagct ggttatggat catgctattc ctgcggggga cagagggcta
     1981 ctgacgctaa tttctgctgc tctgatgttt tttatattac tcaaagctgc aacgagtacg
     2041 ctgcgcgcat ggtcttcact ggttatgagc acgctcatca atgtacagtg gcagtcgggg
     2101 ctgttcgatc atcttctcag actaccgctg gcgttttttg aacgccgaaa attaggtgat
     2161 atccagtcac gttttgactc ccttgacaca ttgagggcca catttaccac cagtgtgatc
     2221 gggttcataa tggacagcat tatggttgtc ggtgtttttg tgatgatgct gttatacgga
     2281 ggatatctca cctggatagt tctctgcttt accacaattt acatttttat tcgtctggtg
     2341 acatacggca attaccgaca gatatcagaa gaatgtcttg tcagggaggc ccgtgccgcc
     2401 tcctatttta tggaaacatt atatggtatt gccacggtaa aaatccaggg gatggtcgga
     2461 attcgggggg cacactggct taatatgaaa atagatgcga taaattcggg tattaagcta
     2521 accagaatgg atttgctctt cggaggaata aatacctttg ttaccgcctg tgatcagatt
     2581 gtaattttat ggctgggagc aggccttgtg atcgataatc agatgacaat aggaatgttt
     2641 gtagcgttta gttcttttcg tgggcagttt tcggaaagag ttgcctctct gaccagtttt
     2701 cttcttcagc taagaataat gagtctgcac aatgagcgca ttgcagatat tgcattacat
     2761 gaaaaggagg aaaagaaacc tgaaattgaa a
//
LOCUS       DAFAVF010000280         2738 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFAVF010000280 DAFAVF010000000
VERSION     DAFAVF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.284"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..129)
                     /locus_tag="LGK42_004462"
     CDS             complement(<1..129)
                     /locus_tag="LGK42_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HBK2660611.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAE"
     gene            complement(126..806)
                     /locus_tag="LGK42_004463"
     CDS             complement(126..806)
                     /locus_tag="LGK42_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK2660612.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(803..1588)
                     /gene="bet"
                     /locus_tag="LGK42_004464"
     CDS             complement(803..1588)
                     /gene="bet"
                     /locus_tag="LGK42_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBK2660613.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWND
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            complement(1594..1890)
                     /locus_tag="LGK42_004465"
     CDS             complement(1594..1890)
                     /locus_tag="LGK42_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBK2660614.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(1967..2173)
                     /locus_tag="LGK42_004466"
     CDS             complement(1967..2173)
                     /locus_tag="LGK42_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBK2660615.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTSDLLVEVFLDGRGNGLNH"
BASE COUNT          631 a          691 c          701 g          715 t
ORIGIN      
        1 ctcagccagc cgctgggctt tcagtggatt tctgataaca gaaaggccgg gaaataccca
       61 gcctcgcttt gtaatggagt agacgaaagt gatcgcgcct acccggatat tatcgtgagg
      121 atgcgtcatc gccattgctc cccaaataca aaaccaattt cagccagtgc ctcgtccatt
      181 ttttcgatga actccggcac catctcgtca aaactcgcca tgtacttttc atcccgctca
      241 accacgacat aatgcagtcc ttcacgcttc atacgcgggt catagttggc aaagtaccag
      301 gcatcttttc gtgtcaccca catgctgtac tgcacctggg ccatgtaagc cgactttatg
      361 gcctcgaaac caccgagccg gaacttcatg aaatcccggg aggtaaacgg gcatttcagc
      421 tcaaggccat tgccgtcact gcataaacca tcgggagagc aggcggtgcg catactttcg
      481 tcgcgataga tgatcgggga ttcagtaaca ttcacgccgg aagtgaattc aaacagagtt
      541 ctggcgtcgt tctcgtactg ttttccccat gccagcgcct tagcattaac ttccggagcc
      601 acaccggtgc aaacctcagc cagcagggtg tggaagtagg acattttcat gtcaggccac
      661 ttctttcctg agcggggctt ggctatcacg ttgtgaactt ctgaagctgt gatgacgccg
      721 agccgtaatt tgtgccacgc atcatccccc tgctcgacag ctctcacgtc gatcccggta
      781 cgctgcagga taatgtccgg tgtcatgcag ccaccttctg ttcagaggct ttctgtttca
      841 ggaaaccaag agctttcact gcttcggcct gtgtcagttc tgacgatgcg cgaatgtcgc
      901 ggcgaaatat ctgggaacag agcggcaata agtcgtcatt ccatgtttta tccagggcga
      961 tcagcagagt gttaatctcc tgcatggttt catcgttaac cggagtgatg tcgcgttccg
     1021 gctgacgttc tgcagtgtat gcggtatttt cgacaatgcg ctcggcttca tccttgtcat
     1081 agataccagc aaatccgaag gcgagacggg cacactgaat catggcttta tgccgtaaca
     1141 tccgtttggg atgcgactgc cacggccccg tgatttctct gccttcgcgg gttttgaatg
     1201 gttcgcggcg gcattcatcc atccactcgg taacgcagat cggatgattg cggtccttgc
     1261 ggtaaatccg gcatgtgcag gattcattgt cctgctcaaa gtccatgcca tcaaactgct
     1321 ggttttcatt gatgatacgg gaccagccat caacgcccac caccggaacg atgccgttct
     1381 gcttgtcagg gaaggcgtaa atttctttcg tccacggatt aaggccgtac tggttggcga
     1441 cgatcagcaa tgcgatgaac tgcgcatcgc tggcatcacc tttaaatgcc gtctggcgaa
     1501 gagtggtgat cagttcctgt gggtcgacag aatccatgcc gacgcgttca gccagcttcc
     1561 cagccagcgt tgcgagtgct gtactcatcc gttttatacc tctgaatcaa tatcaacctg
     1621 gtggtgagca atggtttcaa ccatgtaccg gatgtgttct gccatgcgct cctgaaactc
     1681 aacatcgtca tcaaacgcac gggtaatggc ttttttgctg gccccgtggc gttgtaaatg
     1741 atcgatgcag agtgattcaa acaggtgctg gggcagacct ttttccatgt cgtctgccag
     1801 ttctgcctct ttctcttcac gggcgagctg ctggtagtga cgcgcccagc tctgagcctc
     1861 aagacgatcc tgaatgtaat aagcgttcat ggctgaactc ctgaaaatgg ctgtgaaaat
     1921 atcgcccgcg aaatgccagg ctgattagga aaacaggaaa ggggggttag tgattcaggc
     1981 cgttaccgcg tccgtcgaga aaaacttcca cgagcaaatc actggtataa gtgcgctcga
     2041 tgccgcgatg cagataaagc cgtccgcgta aattagctga tgcagtccag gtaccatctt
     2101 tgtgtttgac cagcatgcct ggcatgaccg caccgcgatt aacggtctgc gttccgtaat
     2161 gttgatgaac cataaaaact cctgcccgta agctgggctg ctgaacatat agagacttct
     2221 gcgcgtattc aggcggtgga tggccgccgg ttgtcataac taagccgcct cgttgaagcg
     2281 actgaggtat aaagtgttga gttgatttca gctggtcaca ccgacgttca cgcgtccgct
     2341 tcacccctcg cactccccgg agcctgctga aattcaagct gcggatctaa gcggtcatcg
     2401 caacagtgaa tcaggtggtt gccgtatcgt tgtgttgttg cgatatggta ataatagcta
     2461 ttgctattgg tgacatcaat acttattgct attaattaat gtgttttgat atcaaatgtt
     2521 tgatagcaaa aagaatttat tttgtgactt gcatcgcata gcgataactg aagcgaggtt
     2581 gtggtggttt tttgaacggt gtgtgatgag gggaggcaaa aaaaacccgg cacggtggcc
     2641 gggttagttg tgagcattag gctgctgtgt ttaaaataat acttgcagct tgttctacag
     2701 agtttacagc tgtaatagat atatcgtaag tataagca
//
LOCUS       DAFAVF010000281         2738 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFAVF010000281 DAFAVF010000000
VERSION     DAFAVF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.285"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            258..1871
                     /gene="mppA"
                     /locus_tag="LGK42_004467"
     CDS             258..1871
                     /gene="mppA"
                     /locus_tag="LGK42_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBK2660616.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1922..>2738)
                     /gene="ynaI"
                     /locus_tag="LGK42_004468"
     CDS             complement(1922..>2738)
                     /gene="ynaI"
                     /locus_tag="LGK42_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBK2660617.1"
                     /translation="ITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSAR
                     IMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLY
                     FDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRM
                     TNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMV
                     YCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          777 a          614 c          660 g          687 t
ORIGIN      
        1 cttcgtacaa tggcgacaga tgctgatttt gataccgaaa actggtttga acgtgcgaag
       61 cctgaacgta gtgttcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca
      121 ctaaatctga tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt
      181 gatggaagtt gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt
      241 cggttagagg gaaacttatg aagcactctg tttcagtcac gtgttgtgcg ctgttggtca
      301 gcagcatttc tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc
      361 aggagctggt gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg
      421 gcctgccaga gattcaggtc attcgcgatc tgtttgaagg actggtgaat cagaacgaga
      481 aaggggagat tgtccccggc gttgcgactc agtggaaaag taatgacaac cgtatctgga
      541 cttttaccct gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt
      601 ttgtctacag ctggcagcgt ctggtggacc cgaaaacgct atcgccattt gcatggtttg
      661 ccgcgctggc gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgacc
      721 agcttggcgt caccgcagtt gatgcccata ccttgaaaat tcagcttgat aaaccgttgc
      781 cgtggtttgt gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag
      841 aaagcggtaa agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta
      901 aagatcgcgt agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg
      961 ccaaaacggt actgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta
     1021 agcgttacct cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga
     1081 agctgttgaa ggatattccg gggcaggttt atacgccgcc acagctcggg acctattatt
     1141 atgcgtttaa cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta
     1201 tgacgataga tcgccgcctg atgaccgaaa aagtgttggg aacgggcgaa aagccagcgt
     1261 ggcattttac accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa
     1321 tgagtcagga agaactgaat gcacaggcaa aaactttgtt gagcgcagct ggttatggtc
     1381 cgcaaaaacc gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg
     1441 cgattgctgt agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc
     1501 aggaatggaa aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgtgcct
     1561 catgggtggg ggattataat gaaccctcca ctttcctgac attattaacg tcaacgcatt
     1621 caggaaatat ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga
     1681 cggaaaatac cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg
     1741 agcaagcacc gattgcacca atttatcaat ataccaatgg acgattaatc aagccgtggc
     1801 tgaaaggtta tcccattaat aacccggaag atgtggcgta cagccggact atgtatattg
     1861 tgaagcattg atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga
     1921 tttagcgacc ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg
     1981 caaagtccgc gccgtgtgac tgtacaatat cgataatctt caaataaacg tcttgctgtg
     2041 cagcaagcca ctcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg
     2101 acgagtcggc aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt
     2161 gatttttcag catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat
     2221 aacgtaaacc aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc
     2281 tgatcgatga aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt
     2341 tggtaattcg ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga
     2401 tccagtcgcc aatactgaaa ggacggtcga aatagagcat gatcccggaa aagaaattac
     2461 tcagaatatc tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca
     2521 agccagaaag gctcatgccg aaatgttcgc cataaagcag aacaagcacc acaataatgg
     2581 tgattttgat aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct
     2641 gctgtttttc aaactgatta atcagcaaaa atagcttaat cgtcagaata accgcaatca
     2701 gggacgtaca gataaaatcg ataacgcctg gggtgata
//
LOCUS       DAFAVF010000282         2724 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFAVF010000282 DAFAVF010000000
VERSION     DAFAVF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.286"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            137..424
                     /locus_tag="LGK42_004469"
     CDS             137..424
                     /locus_tag="LGK42_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660618.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            542..1363
                     /locus_tag="LGK42_004470"
     CDS             542..1363
                     /locus_tag="LGK42_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBK2660619.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRIRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1660..2262)
                     /locus_tag="LGK42_004471"
     CDS             complement(1660..2262)
                     /locus_tag="LGK42_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBK2660620.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            2583..>2724
                     /locus_tag="LGK42_004472"
     CDS             2583..>2724
                     /locus_tag="LGK42_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HBK2660621.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGL"
BASE COUNT          750 a          560 c          654 g          760 t
ORIGIN      
        1 tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaaa ggcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaaccaga
      121 gagaggagaa agcaggatga gtacccgaaa tatccacgtt aacaccgcgt cgtacacgct
      181 tcttgttgcc gggaagaaaa aaaacaccgg ggaagagtgg gacgtcctgg aattcagcag
      241 cctgactgag ctgaaaaaat atcgcaaaag ccacccggaa aagatggcct tcagttatag
      301 ctacgcgctc agtcagggtg tggataagca gttccgccat atcaacattg cggaagccga
      361 tcacttcaaa cagttcctgc gtcagattaa gcgtgccggc ctcgatatcc gggcgatctg
      421 ctgaccggat gtgatgaata tccctcctga ttgttgcaat cttgttaatg gagagcgggg
      481 ggatgatatt aagcggaaaa attttttatt aaatttattc tttcataaag gagtagctgt
      541 tatgcgatta gcttcccgtt ttggtcggta taattccatc cgccgtgaac gtcctttaac
      601 ggatgatgaa ttaatgcagt tcgtgccttc ggtattttcc ggtgataaac atgagtcccg
      661 gagtgaacgt tatacgtata ttccaacaat caatatcatc aataagttac gtgatgaagg
      721 tttccagcca ttctttgcct gtcagagtcg gattcgtgat ttgggacgtc gcgaatacag
      781 taaacatatg ttacgtcttc gcagggaagg gcatattaac ggacaggaag ttcctgaaat
      841 tatcctgctt aattcacatg atggttcatc cagttatcag atgatccccg gaatttttcg
      901 ttttgtctgc acaaatggcc tggtgtgcgg aaataatttt ggcgaaatcc gcgttccaca
      961 taaaggtgat attgtcgggc aggttatcga gggagcgtat gaagtgctcg gtgtctttga
     1021 taaggtcact gataatatgg aggcgatgaa agaaattcat cttaacagtg acgagcaaca
     1081 tttatttggc agagctgcac tgatggtcag gtatgaagat gaaaataaaa cgccagtgac
     1141 acctgaacag ataattactc cccgtcgtcg ggaagacaaa cagaacgatc tctggacaac
     1201 ctggcagcgg gttcaggaga atatgataaa aggtggatta tcggggcgaa gtgcctccgg
     1261 gaaaaatacc aggacaagag ccattacagg tattgatggt gatataagaa tcaacaaggc
     1321 gttatgggtg attgccgaac agttcagaaa gtggaagtca tgataatata agtaaagccc
     1381 cgaaaaaatt ttcggggctt tacttatatt atcatttttc ggcattgctg tgtcgtctga
     1441 tatacctcaa tttcatcaac cggctttccg gttgtctgta aatattgtgc ccgcgaaaaa
     1501 tggtctggca ggaggctgca cagttaccgg gtatatataa gatgggttcc cgcttcgcgg
     1561 ggcttcggcc agtcagtatt gtcactcata tgatattttt gtgtgtggcc ttccatgccg
     1621 cttttgcggc ataaccggta tctgacaatg tctgtaagat taattgttct gcacgctgtt
     1681 aatttccgga agtgatttct tcgtggccgg aagccggata cctttactgc tttttatccc
     1741 agtataaatc cggtaaacat ctgatgcgta ggcaagacgt ctctggttct ggcgttcggt
     1801 tttcctgaat ccggcattgt atgcaccaac ggcctcccag gtgacgcccc attttttaaa
     1861 tgctattgcc agataataag caccggtata aatgttcatg cagggatctg ttgtcagatg
     1921 ttctggctta attccgtagc gggccagttc gttaaaatgc tgggaatcta cctgcatcag
     1981 tccgctgcca tatcccgtta ccggattaat accgatggca ttaacccggt aacgggattc
     2041 ttgccatgat attgctctca gtaaatcagg atctattttg taatcccggc ctgcaagatc
     2101 aaagcaatca gtggcctggc agatctcatt tataaacatc aggcagatgg ctaacatcca
     2161 ttttttcatt tttccacctc tggtgacttt atccgtaaat aatttaaccc actccacaaa
     2221 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
     2281 tttttgctga tttttcttta taaatagaga gttatgaaaa attagtttct cttactctct
     2341 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgctttgt
     2401 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
     2461 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
     2521 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
     2581 taatggctaa ggtgaacctg tatatcagca atgatgccta tgaaaaaata aatgcgatta
     2641 ttgagaagcg tcgacaggaa ggggcaaggg aaaaagatgt cagtttttca gcaacagctt
     2701 caatgcttct tgaactgggg cttc
//
LOCUS       DAFAVF010000283         2699 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFAVF010000283 DAFAVF010000000
VERSION     DAFAVF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.287"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            373..1584
                     /gene="bluF"
                     /locus_tag="LGK42_004473"
     CDS             373..1584
                     /gene="bluF"
                     /locus_tag="LGK42_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBK2660622.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1789..2520
                     /gene="bluR"
                     /locus_tag="LGK42_004474"
     CDS             1789..2520
                     /gene="bluR"
                     /locus_tag="LGK42_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBK2660623.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          786 a          570 c          581 g          762 t
ORIGIN      
        1 tttgcaaagt aacgagtgat tgctcccgca gaatctaaag tcactgaatt ttgatgcatg
       61 ctacgcctct gttaaaaatg ttaatcaatg ttaagtgagg taacaatagc aaacttgtac
      121 gaaatatgtg tacagaatga taaattaatt ttttgcaatt agtgctaatg catataaaac
      181 aatgtgttat gataaaacat attgcatcat ttaactgaca atctatacaa gatttttcgc
      241 caactagtgt tcaacttttt ccatttttta tcaatgtaaa gaaacggtta tgttttggta
      301 ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct cacccattac
      361 cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct
      421 gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta
      481 acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa
      541 caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag
      601 ctgctgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat
      661 ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa
      721 tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc
      781 gaacaatcaa cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga
      841 tctgataaag aacttgattc ctgtgccctt tcaccaacta taaacgacca ctttgccttt
      901 catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat tgtgcaaaaa
      961 aatgaagata gcccatcagc catagcggtt gggcagcgta aagacgggga aatctacaca
     1021 gcggatctca aaagtaaggc gcttgcattc acgatggcac acgcacttga gctcggtgat
     1081 aaaatgattt caatcaatct attacctatg accctggtta acgaacctga cgcagtctct
     1141 tttttactta atgaaataaa ggccaatgct ctggtgcctg aacaaatcat cgttgaattt
     1201 actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcgattaa atcgctaaag
     1261 gctgctggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg tttgttactc
     1321 ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac caatgttcat
     1381 aaaagtgggc cacggcaggc aattattcag gcgatcataa aatgctgtac atcacttgaa
     1441 attcaagtca gtgctatggg cgtggcaaca ccagaagagt ggatgtggct tgaatccgca
     1501 ggaattgaga tgtttcaggg agatctgttt gctaaagcta aattgaatgg tatcccttcc
     1561 atagcgtggc cagagaaaaa ataattttca gcacattctt tcacatgatt tcagtaaatc
     1621 atcgtgaaaa tgaatacgtt acgtttaaat ttaatccaca ttaaaaataa tgtttcatcc
     1681 aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt acaaagtatt
     1741 attgggtcgt gtacaggcga cggagatttg tgaccgcaag gaggaattgt ggcttattac
     1801 agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg ggcctggcaa
     1861 cgccgctacg gtttgttaaa accacagcgc agtgaaggcg gacaccgact ctttgatgaa
     1921 gaagacatac aacgcatcga agagatcaag cgttggataa gtaatggcgt ccctgtaggc
     1981 aaagttaaag cattactgga aaccaccagc caggatacgg aagatgactg gagccgcctg
     2041 caagaagaga tgatgtcaat tcttcgcatg gctaatcctg ccaaactacg cgcgagaatt
     2101 atttcactgg gtcgagagta cccagtagat caattgatta atcatgttta ccttcctgtt
     2161 cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta tgagcagtat gtttgacggc
     2221 gcattaattg aatacgcagc aacctcgctt ttcgaaatgc gccgtaagcc cggtaaagaa
     2281 gccattctga tggcgtggaa tgttgaagag agagcacgat tgtggctgga agcatggcgt
     2341 ttatcattgt caggatggca catttctgtt cttgctgatc ccattgaatc gccgcgcccg
     2401 gaactgttcc cgacgcaaac attgattgtc tggacaggca tggcaccaac gagaaggcaa
     2461 aacgaacttt tacaacattg gggtgagcaa ggttataaag tcatctttca tgccccctaa
     2521 tttcaccttt atcccgcccc ttagacagaa tgttataatc tgaacccaca acagcgtttg
     2581 taccggagct ggtccgttgc aaaatgctca ccgggagaat tcagatattc cctctatcac
     2641 actctgtaca tatcacagcc aaaggacaga ccagattgaa gtggtaaaac cgatcgtgc
//
LOCUS       DAFAVF010000284         2649 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFAVF010000284 DAFAVF010000000
VERSION     DAFAVF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.288"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            117..1049
                     /locus_tag="LGK42_004475"
     CDS             117..1049
                     /locus_tag="LGK42_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK2660624.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1090..2520)
                     /locus_tag="LGK42_004476"
     CDS             complement(1090..2520)
                     /locus_tag="LGK42_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBK2660625.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          701 a          571 c          549 g          828 t
ORIGIN      
        1 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
       61 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
      121 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
      181 gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga
      241 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
      301 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
      361 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
      421 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
      481 tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct
      541 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccag
      601 agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
      661 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa
      721 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
      781 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
      841 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc gtacatgtgg
      901 atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc
      961 gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt ctgacgtcgt
     1021 tgatgtcatc acagcggcag gacaagtaaa ttttgctcgc ctctgaatga gcagaggcga
     1081 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc
     1141 ctgtccgtta aaaacaactt tacccggtgt ataacgcagt tgcagcgagg cggtattgtt
     1201 gtcaatcgtg attaactgaa acatctgacc taaggtcatc atcccgctga tttgtttatc
     1261 agcccgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt
     1321 ttctgtggcg acattaagcg gtaacttcac atcaaaattg agcgatttaa tatctttgtt
     1381 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
     1441 gccgagtgcg ttcttccatt ttaccggttg tttaatggtc ggctcacttt tttgtaataa
     1501 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
     1561 taactcaggg tgtgcagcaa gctgcttctg cattgcaatg ttatactgga tgataaattg
     1621 gcgcaccgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
     1681 ctgattccct tttgttaact tatcaagttc gtaggtgaga ttgacattga tataatcttt
     1741 gtctttaacg cgatccagca tggtttttgc aacgaaagca tcgatttggg caacgtcttc
     1801 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
     1861 cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
     1921 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
     1981 actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc
     2041 aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
     2101 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
     2161 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
     2221 caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag
     2281 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
     2341 gtcaaaattt tgataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc
     2401 gttaaaatct ttaataaatt tttcgacacc aggctgaatt tgcgtacctg tgtaccatgt
     2461 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
     2521 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
     2581 cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa aaaagcccgt
     2641 gaatattca
//
LOCUS       DAFAVF010000285         2628 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFAVF010000285 DAFAVF010000000
VERSION     DAFAVF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.289"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..281)
                     /locus_tag="LGK42_004477"
     CDS             complement(<1..281)
                     /locus_tag="LGK42_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBK2660626.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAAS"
     gene            complement(291..1610)
                     /locus_tag="LGK42_004478"
     CDS             complement(291..1610)
                     /locus_tag="LGK42_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBK2660627.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAMYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1591..>2628)
                     /locus_tag="LGK42_004479"
     CDS             complement(1591..>2628)
                     /locus_tag="LGK42_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2660628.1"
                     /translation="CRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRAS
                     FIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQS
                     AMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTD
                     NGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASR
                     QASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEA
                     VMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQS
                     TEEEKSDSRAA"
BASE COUNT          501 a          846 c          719 g          562 t
ORIGIN      
        1 tcgctggcag cctccggcca gagcacatcc tcataacgga acgtgccgga cttgtagaac
       61 gtcagcgtgg tgctggtctg gtcagcatca accgccagaa tgccaacggc agtaccgtcg
      121 gtggtgccat cccacgcaac cagcttacgg gtggaggtgt ccagcatcag cggggtcatt
      181 gcaggcgctt tcgcactcaa tccgccgggc gcggttgccg tatgtgccgg gtcactgttg
      241 cccagcggct ggtaatgggt aaaggtttct ttgctcgtca taaacatccc ttacactggt
      301 gtgttcagca aatcgttaac ggcatcagat gccgggttac ctgcagccag tggtgccggt
      361 gccccctgca tcagacgatc cagcgcagtg tcactgcgcg cctgtgcact ctgtggtgca
      421 gctgccagaa tgcggcgggc cgtttccacg gtcataccgg gggtttcggc cagcacacgt
      481 gcctgttctt cgcgtccgtg agcttcctca cagttgagga tccccataat gcggctgttt
      541 tctgccgcaa ccgcagcggt gatctgcgcg ttcacgtccg gctgcgccgc gctggcgttt
      601 tcgccctccg tcgcttgcac cacgtcagta acgtcagcct gcgaagcagt ggctgaaaca
      661 gttgttgatt gagtctcttt ggtcattcgc cctcctgaga gacgggattt acgtgcatcc
      721 agtgcatcac gcataacggt gatcgcatcg gtgctgttga caagttcatc agccagtccg
      781 gcatcaatgg cctcctgacc gctgtacatt gcagcctcgg tatccagcac agcctgcacg
      841 gacaggccgg tatatgccga caccttctgc gcaaacatcc ggcgggttgc atccatccgg
      901 gactgcagtg tctcccggac atcacccggt agatggctgt aggggttgcc atccacctta
      961 tggctgccgc tgtaaatcag cgtgatttcc acgccctgtt tctccagcgc agcgccgtaa
     1021 ttactgtgag ccatcatgac gccgatggag cctgtccggg cggtctgcgt gaccagacgc
     1081 cgggaggcgg cgctggcaag cagctgacct gcactgcagt tcatgtcgtt ggccagcgcc
     1141 cataccggct ttatgtcacg cacacgggcg atgatgtcag cacagtcaaa tgctcccgcc
     1201 accatcccgc ccggtgtgtc catatcgagc agaatgccgt ccaccatcgg atcgctggca
     1261 gcctgttgca gacgagcgat aatgccgttg taaccggtca ttcccgaata cggctgcagc
     1321 gcccgcgtcc ggctgaccag cgtaccggac accggcagca cggcgatgcc gttcatgacc
     1381 tgataactgc gggcctgtcg tggtccgtca tcatcaccgg ataacgccag cgccgcgggt
     1441 gcctctccgg cagtcaggct atcgccggat actgcatccg tcaggcgact gatcccaagc
     1501 tggcctgcaa gcgcacaaaa gaaaacccgc gcataggcgg gttcaagcat cagcggctca
     1561 ttaaaagcca tgctggcaat atgcgggaga ttacgcagct ctgctgtcac tcttctcctc
     1621 ctctgttgat tgtcgcagtc cggattcaaa tgccgcagcc gcccaggcgg gcggtttaag
     1681 accggctgca cggcgctcca tcgtttcacg gacctgctgg gcaaaaattt cctgatagtc
     1741 gtcaccgcgt ttcgcgcact ctttctcgta ggtgctcagt ccggcttcta tcagcatcac
     1801 cgcttcctga acttctttca gaccatcgat ggccatacga ccggagccta tccagtcgca
     1861 gttcccccag gcactgcggg cttcctgaaa gctgaagcgc gcttttgaag gtaacgtcac
     1921 cacgcggcga acgatggcct cttcaagcca gcacagaaac atctggctcg cctgacggga
     1981 tgcgacgaat tttcgccgcc ccataaagta cgcccacgac tcgttcgcac tggcccgtgc
     2041 cgtggagtag ctcatctggg cgtaattccg ggaaagctgc tcatacgaga cacccagccc
     2101 ggcagcgata taccgcaaca gtgactgctc aaacacggag tagccgttat ccgtatcctg
     2161 agccgtctgc aggttcagtg agtcccccgg catcaggtgc ggcacttttg cgcctcccag
     2221 acggaccggt gctgcggcgt aatacgcggc aatttcacca atccagcccg tcagcctttc
     2281 ccgctgctcc tgactgttcg cgcccagaat aaaatccatc gctgactgcg tatccagctc
     2341 actttcaatg gtggcggcat acatcgcctt cacaatggcg ctctgcagct gcgtgttctg
     2401 cagcgtgtcg agcatcttca tctgctccat cacgctgtaa aacacatttg caccgcgggt
     2461 ctgcccgtcc tccacgggtt caaaaacgtg aatgaacgag gcgcgcccgc cgggtaactc
     2521 acggggtatc catgtccatt tctgcggcat ccagccagga tacccgtcct cgctgacgta
     2581 atatcccagc gccgcaccgc tgtcattaat ctgcacaccg gcacggca
//
LOCUS       DAFAVF010000286         2602 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFAVF010000286 DAFAVF010000000
VERSION     DAFAVF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.290"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1409
                     /gene="evgS"
                     /locus_tag="LGK42_004480"
     CDS             <1..1409
                     /gene="evgS"
                     /locus_tag="LGK42_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBK2660629.1"
                     /translation="IMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDK
                     IESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQ
                     VLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYS
                     QTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATIEA
                     KAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDL
                     LITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTL
                     DVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAF
                     QALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVK
                     EHIAELDQEIAVFCQKND"
     gene            complement(1465..2559)
                     /gene="yfdE"
                     /locus_tag="LGK42_004481"
     CDS             complement(1465..2559)
                     /gene="yfdE"
                     /locus_tag="LGK42_004481"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006815732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBK2660630.1"
                     /translation="MTHVLNGPFGTQLLCNMGARVIKVEPPGHGDDTRTFGPYVDGQS
                     LYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFRPGTMEKLGFSWETLQEI
                     NPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGG
                     VYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPY
                     MAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQY
                     IERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMPGNPI
                     KISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT          752 a          616 c          538 g          696 t
ORIGIN      
        1 ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg
       61 aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc
      121 ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc
      181 ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg
      241 cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt
      301 ttaagcaggt cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag
      361 taaaaattac gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga
      421 ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca
      481 gccaaacaag tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag
      541 aattaattaa aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa
      601 catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactatc gaggcaaaag
      661 cagaacaccc catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga
      721 ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca
      781 ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg
      841 acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt
      901 cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt
      961 taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac
     1021 atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg
     1081 aagccctgaa aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt
     1141 tccagcatga aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata
     1201 acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc
     1261 aaaagttgat taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc
     1321 ctgaaattct tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga
     1381 ttgctgtttt ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc aaatgtggga
     1441 gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg
     1501 ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat
     1561 cggattaccc ggcatcatta ttcccccggc ttcaatcaac atatttcttg cctgagtttg
     1621 tggcaattta atggcctcag ccacacttaa taacggcgcg acgggtacac caacttcatg
     1681 tattctggct aaccaaactt cagctgcctg cgtttttaac gtccgctcaa tatattgttt
     1741 aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag
     1801 ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat
     1861 cgtaatcggc ttatcctgag tattgaaaac atcaaaaggt gccatgtagg gatggcgatt
     1921 tcccagacgt tgtggtgact tccctgtcgc gatatatgcc atcagaccat gctccagaaa
     1981 actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg
     2041 gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag
     2101 agatgtacca acgcgcactg gcggagcatc aggatatcct gtttccatca ttatcccgct
     2161 cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa
     2221 acctgacgat gaagcatata tgaggcgcgg gttgatttct tgaagcgttt cccatgaaaa
     2281 ccccagtttt tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag
     2341 catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt
     2401 gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg
     2461 ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag
     2521 aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc
     2581 aaacggccct ttgctttcat ta
//
LOCUS       DAFAVF010000287         2595 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFAVF010000287 DAFAVF010000000
VERSION     DAFAVF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.291"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..188
                     /locus_tag="LGK42_004482"
     CDS             <1..188
                     /locus_tag="LGK42_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBK2660631.1"
                     /translation="IAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAA
                     LDELIPGLLSEYIEQSG"
     gene            163..2088
                     /locus_tag="LGK42_004483"
     CDS             163..2088
                     /locus_tag="LGK42_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBK2660632.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPLLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2085..2291
                     /locus_tag="LGK42_004484"
     CDS             2085..2291
                     /locus_tag="LGK42_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBK2660633.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQAGMTQRRRGPAGFYV"
     gene            2288..>2595
                     /locus_tag="LGK42_004485"
     CDS             2288..>2595
                     /locus_tag="LGK42_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2660634.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     "
BASE COUNT          623 a          656 c          771 g          545 t
ORIGIN      
        1 ggatcgcagg tgaaattgcc agtattctcg acgggatccc cctgtcggtg cagcggcgtt
       61 ttccggaact ggaaaaccga catgttgatt tcctgaaacg ggatatcatc aaagccatga
      121 acaaagcagc cgcgctggat gaactgatac cggggttgct gagtgaatat atcgaacagt
      181 caggttaaca ggctgcggca ttttgtccgc gccgggcttc gctcactgtt caggccggag
      241 ccacagaccg ccgttgaatg ggcggatgct aattactatc tcccgaaaga atccgcatac
      301 caggaagggc gctgggaaac actgcccttt cagcgggcca tcatgaatgc gatgggcagc
      361 gactacatcc gcgaggtgaa tgtggtgaag tctgcccgtg ttggttattc caaaatgctg
      421 ttgggtgttt atgcctactt catagagcat aagcagcgca acacacttat ctggttgccg
      481 acggatggtg atgccgagaa ctttatgaaa acccacgttg agccgaccat ccgcgatatt
      541 ccgttgctgc tggcgctggc tccgtggtat ggcaaaaagc accgggataa cacgctcacc
      601 atgaagcgtt tttccaatgg tcgtggcttc tggtgcctgg gcggtaaagc ggcaaaaaac
      661 taccgtgaaa agtcggtgga tgtggcgggt tatgatgaac ttgctgcctt tgatgaggat
      721 attgaacagg aaggctctcc gacgttcctt ggcgacaaac gtattgaagg ctcggtctgg
      781 ccaaagtcca tccgtggctc cacccccaaa gtgagaggca cctgccagat tgagcgtgca
      841 gccagtgaat ccccgcattt tatgcgtttt catgttgcct gcccgcactg cggggaggag
      901 cagtacctta aatttggcga taaagagacg ccgtttggcc tcaaatggac gccggatgat
      961 ccctccagcg tgttttatct ctgcgaacat aatgcctgcg tcatccgcca gcaggagctg
     1021 gacttcactg atgcccgtta tatctgcgaa aagaccggga tctggacccg tgatggcatt
     1081 ctctggtttt cgtcatccgg tgaagagatt gaaccgcctg acagcgtgac ctttcacatc
     1141 tggacggcgt acagcccgtt caccacctgg gtgcagattg tcaaagactg gatgaagacg
     1201 aaaggggata cgggaaaacg taaaaccttc gtgaacacca cgctcggtga gacgtgggaa
     1261 gcgaaaatcg gcgaacgtcc ggatgctgaa gtgatggcag agcggaaaga gcattattca
     1321 gcgcccgttc ctgaccgtgt ggcttacctg actgccggta tcgactccca gctggaccgc
     1381 tacgaaatgc gcgtatgggg atgggggccg ggtgaggaaa gctggctgat tgaccggcag
     1441 attattatgg gtcgccacga cgatgaacag acgctgctgc gtgtggatga ggccatcaat
     1501 aaaacctata cccgccggaa tggtgcagaa atgtcggtat cccgtatctg ctgggatact
     1561 ggcgggattg acccgaccat tgtgtatgaa cgctcgaaaa aacatgggct gttccgggtg
     1621 atccccatta aaggggcatc cgtctacgga aagccggtgg ccagcatgcc acgtaagcga
     1681 aacaaaaacg gggtttacct taccgaaatc ggtacggata ccgcgaaaga gcagatttat
     1741 aaccgcttca cactgacgcc ggaaggggat gaaccgcttc ccggtgccgt tcacttcccg
     1801 aataacccgg atatttttga tctgaccgaa gcgcagcagc tgactgctga agagcaggtc
     1861 gaaaaatggg tggatggcag gaaaaaaata ctgtgggaca gcaaaaagcg acgcaatgag
     1921 gcgctcgact gcttcgttta tgcgctggcg gcgctgcgca tcagtatttc ccgctggcag
     1981 ctggatctca gtgcactgct ggcgagcctg caggaagagg atggtgcagc aaccaacaag
     2041 aaaacactgg cagattacgc ccgtgcctta tccggagagg atgaatgacg cgacaggaag
     2101 aacttgccgc tgcccgtgcg gcactgcatg acctgatgac aggtaaacgg gtggcaacgg
     2161 tacagaaaga cggacggcga gtggagttta cggccacttc cgtgtctgac ctgaaaaaat
     2221 acattgcgga gctggaagtg caggccggca tgacacagcg acgcagggga cctgcaggat
     2281 tttatgtatg aaaacgtcca ccattcccac ccttctgggg ccggacggca tgacatcgct
     2341 gcgtgaatat gccggttatc acggcggtgg cagcggattt ggtgggcagt tgcgggcgtg
     2401 gaacccaccg ggtgaaagtg tggatgcagc cctgctgccc aattttaccc gtggcaatgc
     2461 ccgtgcagac gatctggtac gcaataacgg ctatgccgcc aacgccatcc agctgcatca
     2521 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     2581 catcggggag gaaga
//
LOCUS       DAFAVF010000288         2592 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFAVF010000288 DAFAVF010000000
VERSION     DAFAVF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.292"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..889)
                     /locus_tag="LGK42_004486"
     CDS             complement(<1..889)
                     /locus_tag="LGK42_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBK2660635.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSEL"
     gene            complement(941..2560)
                     /locus_tag="LGK42_004487"
     CDS             complement(941..2560)
                     /locus_tag="LGK42_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBK2660636.1"
                     /translation="MAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTM
                     DDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFT
                     NGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAAV
                     GKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSP
                     SLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQY
                     QKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLP
                     LNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKD
                     LKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPH
                     NPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGV
                     VREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          707 a          537 c          567 g          781 t
ORIGIN      
        1 tgagttcaga tttattaatg tttccaattt tgtactgtgg atagacaaaa tggtcgcatt
       61 cgtaaatgtc tccattactt tcaacaacca gattatcctt gcaggactcc tggaaaatac
      121 aacttgtatg cccattcccc aaaaaacggc tgacaaagct ttcaaactga cgaatgaaaa
      181 tttcacccac atcgttttta acccattgca taaaaatggt tgacataaac ttgccataag
      241 ccgtgggagg cacagaaaaa tcaatgatac ggaatgtgtt ctcactatga ccactgaaat
      301 caatattcgg cgtcccggtt tctagcaatt cgataaattg catatgttta ctgccgatag
      361 attttaaaaa atgataaacc tcaagagggt aatggacatt aacgttatta atgacggtta
      421 acgtattaaa ctctacttga tatgatttca gacgctcgat ggctgctatc acttttgcaa
      481 aagtaccgtt acctgaatta ctgcgtctgt aacggtcatg taactcctgg gggccatcga
      541 tcgagatacc aaccagaaat tcatgttctt tgagaaaggc acaccattca ttattcaata
      601 aaatgccatt cgtttgtaat gcattaaaaa tacgtttttg gcctgcatag cgttgttgat
      661 agtgaataac tttacggaaa aaatccaggc cagccagagt gggttcaccg ccttgccagg
      721 taaaatagac ctgattgcca gacgctgcga tatattgttt gatgaactct ttcagagtac
      781 tgtcatccat ccatttttca tgagtaaact gcgactcttt ttcaaggtaa aaacagtaat
      841 cacatttgag attacattga aaactggagg gcttggctgt aacgtgcatc gctatctcgc
      901 tcaataaggc ggcggaaaat tccgccgcat gaaggtttag ttatttcgct tcgcttagtg
      961 ctttcttgat attgttaaac ttctcctgat ttacctcgct aagcggtggc tggctgctgt
     1021 cgataaactc tcttaccacg ccttgcatct ctttaacgac ctgcggattg gcggcggcaa
     1081 ggttatcttt ttgctgtaga tccgtcagtt tgtagagacc taactgattg ttttctactg
     1141 tatagacaag cgaataatcg ttatttctca ccgtataaga gaattggctt aagtcctcag
     1201 tgttggggtt atgcgggtaa tcgtctgact gatggcgaac aaatttgtgg taattatccc
     1261 agaatggaat attttcctcg tcaaaccagt gagaataaga ggttatccag gtcagatttt
     1321 tatgtggctc gccttgtttc ttatcttgca accagggcag caaggaaacg ccatccagct
     1381 taaggtcttt tggaatgctg atatcggctg catcaagagc tgtcgggtag aaatccattg
     1441 cggaaatcag cttgtcataa ttaccgggtt gaagttttcc tttccaccac ataaacattg
     1501 gggtgtgagt accgccagga taggtctgac tcttatagcc tttttgcgcc ccgttcagcg
     1561 gcagaggacc atcgataacc gcaccattat cggaggtaaa gagaataatt gtattgtcat
     1621 actgtccgtt tttcttcagt tgttcgagaa tgcgttttac accctgatca acagaataaa
     1681 cggaagcgta gtagttatct gctgtttgac taccggtatt aaattgcttc tgatattgat
     1741 ccggtgcagg attatcattt ggcaggtgcg gagcattata agccaggtaa agcataaatg
     1801 gctggtcaag tgttttggca cgatcaacaa cgccaattgc ctcatcggtt aactgatcgc
     1861 tgatataacc ttttgcgggg acacgttcac gatttttgaa cagtgaaggg gagttgtaat
     1921 atgccgttcc tgcagcgtgg aatcccataa agtaatcaaa gccacggttt tgaggttgcc
     1981 attcttccgc agaaaatgtg gtgaagttgt catgatagtc acgcgtttgt ttatcttccg
     2041 gtaccggcac attactgatt tttgacaagt gccatttacc tactgctgca gtgtaataac
     2101 catgattctg gaataattca ggcaagaaag tttctgttag cggaatacca tcctgagcat
     2161 cggtattgga atagacacca aagcgggcgg gagctcgacc ggtcattatt gcggcacggg
     2221 aggggccgga aacaccgtgt gccacatagc cgttagtaaa ccgtacgcct tcatccatta
     2281 atgaaaggag cgtcggcgtt gatttttgtg cagcttcaat ggctttatct atccctattt
     2341 tgtaggtatc gacaacttca cgattttcca ttgtttttgg gtcaaaagat cccttatcaa
     2401 aaggaagttg tccataacca agatcatcca tggttagtac gataatattt ggctttcctt
     2461 tggtactgta ttcagtcggc gtaaagtctg agaaagcaac gtttgttttg gttgctttca
     2521 gctttacatc atctgccgca tgagcagcaa atgcagccat accagatgcc agtatcaaag
     2581 atatcgaggt ac
//
LOCUS       DAFAVF010000289         2583 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFAVF010000289 DAFAVF010000000
VERSION     DAFAVF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.293"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            67..600
                     /gene="ymdB"
                     /locus_tag="LGK42_004488"
     CDS             67..600
                     /gene="ymdB"
                     /locus_tag="LGK42_004488"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBK2660637.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRSAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            542..2023
                     /gene="clsC"
                     /locus_tag="LGK42_004489"
     CDS             542..2023
                     /gene="clsC"
                     /locus_tag="LGK42_004489"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBK2660638.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDVMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2031..>2583)
                     /locus_tag="LGK42_004490"
     CDS             complement(2031..>2583)
                     /locus_tag="LGK42_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBK2660639.1"
                     /translation="PILSNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTTPSRG
                     CTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNFQSAR
                     VTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEIHLRI
                     PLLKFLFSGKPVVKRENDKAPAR"
BASE COUNT          666 a          600 c          687 g          630 t
ORIGIN      
        1 gaaaaatgcg atttaaccaa aaagcctgct gtacacttaa gaaacaagaa ggtgtaagga
       61 ggcgttatga aaacgcgtat tcatgttgtg cagggtgata ttaccaaact ggccgttgat
      121 gtgattgtga atgcggctaa tccgtcatta atgggaggcg gcggcgtcga tggggccatt
      181 catcgctcag cgggtccggc cctgctggat gcctgtttaa aagtcaggca acagcagggc
      241 gattgcccta cggggcatgc cgttattacg cttgcaggcg ctcttcccgc taaagccgta
      301 gtgcacaccg tcgggccagt ctggcgtggt ggtgaacaaa acgaagacca gcttttgcag
      361 gatgcctatc tcaatagcct acgactggtg gcggcaaaca gctatacgtc agtggctttt
      421 cctgcaatca gtactggggt ttatggttac cctcgtgcgg cagcggctga aatcgcagtt
      481 aaaaccgttt cagaatttat tacccgtcac gctttacccg aacaggtata ctttgtctgt
      541 tatgatgaag aaaacgccca cctctacgaa agactcctta cccaacaagg agatgaatga
      601 tttgccccgg ctggcgagcg cggtgctgcc actgtgttcg caacatcccg gtcagtgtgg
      661 tcttttccct ctggagaaaa gtctggatgc gtttgccgcc cggtatcgtc tggccgaaat
      721 ggcagagcat acgctggatg ttcagtatta catctggcag gacgatatgt cgggtcggtt
      781 actgttttcc gccctgttag ccgcagcaaa gcgtggcgtt cgcgtccgtt tgttgctgga
      841 cgacaacaat acgcccggac ttgacgacat tttacgcttg cttgacagcc atccccgcat
      901 tgaagtccgg ctttttaatc ctttctcgtt tcgcttgctg cgtccgcttg gttatatcac
      961 cgacttttcc cgtctcaatc gccgtatgca caataaaagt ttcactgtcg atggcgtggt
     1021 gacgctggta ggggggcgaa atattggtga tgcctatttt ggggcagggg aagagccact
     1081 tttttcggat ttagatgtta tggcaatagg gcccgtggta gaggacgttg ccgatgattt
     1141 cgcccgctac tggtattgca aatcggtttc acccttacag caagtgctgg atgtcccgga
     1201 aggtgaaatg gcggatcgca tcgagttacc cgcctcctgg cataacgatg ttatgacgca
     1261 tcgttactta cgcaaaatgg aatccagtcc gtttataaat catctggttg atggaacgtt
     1321 gccgcttatc tgggcgaaaa cacgtttatt aagtgatgat ccggcgaaag gggagggcaa
     1381 ggcaaaacgg cattcactgt taccgcagcg cctgttcgat atcatgggct cacccagtga
     1441 acgcatcgat attatctctt cctattttgt accgacacgc gcaggtgtgg cgcaactctt
     1501 acggatggtg agaaaagggg taaagattgc gatcctaacc aattctcttg ccgctaacga
     1561 tgttgctgtc gtccatgccg gatacgcgcg ctggcgcaaa aaattgctcc gctatggcgt
     1621 ggaattatat gaactcaagc cgacgcgtga acaaagtagt acgttacacg atcgcggcat
     1681 aaccggtaat tccggagcca gcctgcatgc taaaaccttt agcatcgatg gtaaaacggt
     1741 gtttatcggc tctttcaatt tcgatccgcg ttcaacattg ctcaatactg aaatgggctt
     1801 cgtgatagag agcgaaacgc tggcacagtt aattgataaa cgctttattc agagccagta
     1861 tgatgcggcc tggcagctcc gtctggacag gtggggacgg atcaactggg ttgatcgtca
     1921 tgcaaagaaa gagattattc tcaaaaaaga acccgccacc agtttctgga agcgggttat
     1981 ggtcagactg gcgtcgatat tgcccgtgga atggttattg taaatgtggc ttaacgggct
     2041 ggtgctttat cgttctcacg cttaacaacc ggtttaccag aaaacaaaaa cttcagtaac
     2101 gggatgcgta aatgaatttc atacagaatt atcgcaatcc ctactacgaa tatcaggcca
     2161 cagagaaaac caagccagtt ggaggtgatg tgcggtgtaa tgtatgcgcc gaaaaacagc
     2221 gttaacgggt ggtgaaccag atagataaat agcgatgcgt taacaaaata agtcacccgc
     2281 gctgactgga agttaagcaa acggtggcca aaggagaaga ccacattcac catccacaga
     2341 ccgaggacca tggtgatcac tgactcggtt tcgtacatcc aggcatcgcc actgccatag
     2401 cgctggttga gtaaataagc gacaaacgcc aatgctgcag cgagggtaca gccacgagac
     2461 ggcgtggtaa acaaggcttt aagatgaggg aaaatgaaag ccagtgcgcc gaggataaag
     2521 aacggtaaat aaaatagcgt ttgcatgaca ataaaattga acatgccatt actcagaatg
     2581 ggc
//
LOCUS       DAFAVF010000290         2529 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFAVF010000290 DAFAVF010000000
VERSION     DAFAVF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.294"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            253..447
                     /locus_tag="LGK42_004491"
     CDS             253..447
                     /locus_tag="LGK42_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBK2660640.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            807..1100
                     /gene="iss"
                     /locus_tag="LGK42_004492"
     CDS             807..1100
                     /gene="iss"
                     /locus_tag="LGK42_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBK2660641.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1191..1373)
                     /gene="rzoD"
                     /locus_tag="LGK42_004493"
     CDS             complement(1191..1373)
                     /gene="rzoD"
                     /locus_tag="LGK42_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HBK2660642.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1590..2087)
                     /gene="rrrD"
                     /locus_tag="LGK42_004494"
     CDS             complement(1590..2087)
                     /gene="rrrD"
                     /locus_tag="LGK42_004494"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBK2660643.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIEVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2087..2302)
                     /gene="essD"
                     /locus_tag="LGK42_004495"
     CDS             complement(2087..2302)
                     /gene="essD"
                     /locus_tag="LGK42_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBK2660644.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          713 a          618 c          549 g          649 t
ORIGIN      
        1 gccttaaacc ggaaaatttt cataaatagc gaaaacccgc gaggtcgccg ccccgtaacc
       61 tgtcggatcg ccggaaagga cccgaaaaat gataataatt atcatctaca tatcacaacg
      121 tgcatctacg ccatcaaacc acgtcaaata atcaattatg acgcaggtat cgtattaatt
      181 gatctgcatc aacttaacgt aaaaacaact tcagacaata caaatcagcg acactgaata
      241 cggggcaacc tcatgtcaac taagaacaga acccgcagaa caacaacccg caacatccgc
      301 tttcctaacc aaatgattga acaaattaac atcgctcttg agcaaaaagg gtctgggaat
      361 ttctcagcct gggtcattga agcctgccgt cggagactaa cgtcagaaaa gagagcatat
      421 acatcaatcc aaagtgatga tgaataaaca tcccggtttc ttccaccatc gcaccggaaa
      481 agcgactatg agggtaaccc tgcgtctgtc agcacagtaa aacccggtgt gcatcgtttt
      541 tgattattcc cgcacactca cgcagaagga attccccgtc gggctacggt catggttaat
      601 gcgggaatac ggcgacgata cagcgcagct aaaagggtaa tggacagaaa gagcggttta
      661 tttcattcca caggattctg agtgcccccc tcctccaata ggctgagcat ccacctatat
      721 agttttaatt ttcatcaatc catttaacta tcgtttaatt gttgtcacat aggattctgc
      781 cgtttttaac aatgcaggat aataagatga aaaaaatgtt gttttctgcc gctctggcaa
      841 tgcttattac aggatgtgct caacagacgt ttactgttgg aaacaaaccg acagcagtaa
      901 caccaaagga aaccatcacc catcacttct tcgtttcggg aattggacag gagaaaactg
      961 ttgatgcagc caaaatttgt ggcggcgcag aaaatgttgt taaaacagaa acccagcaaa
     1021 cattcgtaaa tggattgctc ggttttatta ctttaggcat ttatactccg ctggaagcgc
     1081 gtgtgtattg ctcacaataa ttgcatgagt tgcccatcga tatgggcagc tctatctgca
     1141 ctgctcatta atatacgtct gggttccttc cagttgtttt tgcatagtga tcagcctctc
     1201 tctgagggtg aaataatccc gttcagcggt gtctgccagt cggggggagg ctgcattatc
     1261 cacgccggag gcggtggtgg cttcacgcac tgactgacag actgctttga tgtgcaaccg
     1321 acgacgacca gcggcaacat catcacgcag agcatcattt tcagctttcg catcagctaa
     1381 ctccttcgtg tattttgcat cgagcgcagc aacatcacgc tgacgcatct gcatgtcagt
     1441 aattgccgcg ttcgccagct tcagttctct ggcatttttg tcgcgctggg ctttgtaggt
     1501 aatggcgtta tcacggtaat gattaacagc ccatgacagg cagacgatga tgcagataac
     1561 cagagcggag ataatcgcgg ttactctgtt cattgctgac cccacaaaca gatttcacgc
     1621 tcaatctcac gacgagtcat gagacctttc cattgcttac cgccagcata tgtccagcga
     1681 cgtagctgat cacatgcgcc tttgatatcg ccctggttta ttttgcgaag aagcgtcgat
     1741 gttctgaaat tgccagcacc cacgttgtaa acgaatgagt aaagagcgcc gcgcgttgtt
     1801 tccggtatat cgacttcgat gtacgggtta atttgtctgg cgacagtggc aaggtcttta
     1861 ttcaagagtg ctttgcattc tgctttggta tacgttttac cgagcatgat gtcttttcct
     1921 gtatgcccgt gacatacagt ccatacacca acaatatctt tgtatggtat gtagctgaca
     1981 ccttccagac catcgttacc acttgggcca gtgattaaca ctgatgctat agcaattgct
     2041 ccgccaccaa tagcagcagc aacggctttt cgtaatgatg gaggcattat tcacctctcg
     2101 cagccttgcg cttatcttct ttaatcttga aataaaggtt tgtcaggtac gtcagcaggc
     2161 caaataccag gctacccagc acacctattg ctgcccactg tgagggcgtg actttatcga
     2221 gcagctgtaa aaaccagtaa ccggcactac ctgctgaggt gccataggcg acacccgttg
     2281 ttaacttatc catggatttc ataaccccac ctcgcagaca aagcgggtgt aaattgaggg
     2341 aatacaacgt atcgcaaaaa agcagaaacg taacagactc ggagtcagtg aataactcag
     2401 gtattgggtt atcagctaat atcgagactc aaaaaatgga aaaacccgct cgacggcggg
     2461 tttaagctgt gtgacgaagt aaccactctt aacagcataa ccaatttttt acgtacgtaa
     2521 accactaaa
//
LOCUS       DAFAVF010000291         2522 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFAVF010000291 DAFAVF010000000
VERSION     DAFAVF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.295"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(109..510)
                     /locus_tag="LGK42_004496"
     CDS             complement(109..510)
                     /locus_tag="LGK42_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBK2660645.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            752..1045
                     /locus_tag="LGK42_004497"
     CDS             752..1045
                     /locus_tag="LGK42_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBK2660646.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1117..1776
                     /locus_tag="LGK42_004498"
     CDS             1117..1776
                     /locus_tag="LGK42_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBK2660647.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1868..2314
                     /locus_tag="LGK42_004499"
     CDS             1868..2314
                     /locus_tag="LGK42_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBK2660648.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          650 a          534 c          640 g          698 t
ORIGIN      
        1 cattatgttc tattcggcat tgtctgctat tcgtcattaa cctcttgagc gacagagagg
       61 agtggggcgt taacgtttat gacggggaga gtccccgtcg gttgacattt acttaatttg
      121 aatatcgacg ttatattttt tagttttgtt tttacgggcg tcgtttcggg tttgcagcac
      181 tcgcagctca tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg
      241 tgaatatctg gataaatcaa ctgaatcatc gattcctggc ccgaagaggt aagtatcagc
      301 gccttcatta gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt
      361 atcgtaatcg tatcccttta tttcgccgga gtactgagcg ctgctatggc ctttgctgaa
      421 ctcaacattg acattcttac ccgcagcaaa gctcgtggag gttagcagtg caagcagcaa
      481 tacagcctta ctgatgctct tgattttcat tggtagtctc ctgttcagaa tgagtacgat
      541 gaactgttat aatataacaa ttccttacgg gagaattcca gtggcttttc tgcgttcggt
      601 aaaatcccgg cgcggcattt tttgccgggg ggattactta taaaggggca tggactcaac
      661 aaaccgcaga aatctacagc tggctggctt gtggcgcggg tttcattgat gttagcttat
      721 gccaaaacct ggcatcttaa agagtaaaag tatgttttgt gtgatttatc gaagcagcaa
      781 gcgtgaccag acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga
      841 actgatgaaa ggttttggtc agcctcagtt agcgatgatt ctgccgctgg atgggcgcaa
      901 gaaactggtc aatgctgata ttgaaaaggt taaactggca ttaaccgagc aaggttacta
      961 tttgcagtta ccgccaccac ccgaagattt gctgaagcaa catctttccg tcatggggca
     1021 gaaaacagac gacactaaca aataaccgat atccggcggt ggcattatct ttgtcggcgc
     1081 gggttttcat atccacgata aggtgagggg aacgttatgt atcaacatca taactggcaa
     1141 ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg ttggcagtaa ctatgccaaa
     1201 catattaaag agatgggcag cgcagtgccc gaagagccag tgctgtttat taaaccagaa
     1261 acggcactgt gcgatctgcg gcagccattg gcgatcccat ccgatttcgg ttcagttcat
     1321 catgaagtcg aactggcggt gttgattggc gcgacgctgc gtcaggcaac ggaagagcat
     1381 gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc tgacattgcg tgatgttcag
     1441 ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta aagcgtttga taactcttgt
     1501 ccgctttccg ggtttatccc cgcggcggaa tttaccggcg atccacaaaa tacaacgctg
     1561 ggcctgagcg taaacggcga acaacgccag caaggtacga ctgcggacat gatccataaa
     1621 atcgttccgt tgatcgctta tatgagcaag ttttttaccc tcaaggccgg tgacgttgtg
     1681 ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg gtgatgagct gacagtcact
     1741 ttcgatgggc attctttgac aactcgcgtt ttgtaatact ttttgccgcc tgaaagcggc
     1801 ggcaaaactt gcatcgctgt gccagactgg ttataaggtg cgttttaacg taatggcgga
     1861 acacctgatg agcgatgtac ctttctggca aagtaaaacc ctggacgaaa tgagcgatgc
     1921 ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc ctgcataaac tgatggatga
     1981 agacaccgac gaaatctact tcactaacgt cgcctgtcgc cagctcaata ttaaaacttg
     2041 tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc gactgcatta aattaacccg
     2101 tgaaaatctg ccaacattcg aatggctgcc aatgacctgc gcttatcgtt tgctggcgga
     2161 aggtaaagat ttacctgcgt ggcatccgct acttactggt tcgaaagcgg caatgcatgg
     2221 tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca gaagtgattg actggcagga
     2281 tcatatctta aataaacctg actgggcgca gtgataaatt taatagatgt tgatgggggc
     2341 tttcttaata tacgtctgga tgctcgccat ttcgtagccc tgtttttgaa agcgctctgt
     2401 cataatgaga gttacccgat accttattat caacaggtaa ctctcaatct ttaaaaaacg
     2461 tctgtgtttt taaatgtgat aaaccttata ccttgagtac atggagagaa taatcgctta
     2521 tc
//
LOCUS       DAFAVF010000292         2347 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFAVF010000292 DAFAVF010000000
VERSION     DAFAVF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.297"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            97..600
                     /locus_tag="LGK42_004500"
     CDS             97..600
                     /locus_tag="LGK42_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK2660649.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(646..1062)
                     /gene="rraB"
                     /locus_tag="LGK42_004501"
     CDS             complement(646..1062)
                     /gene="rraB"
                     /locus_tag="LGK42_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBK2660650.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1224..2228
                     /gene="argF"
                     /locus_tag="LGK42_004502"
     CDS             1224..2228
                     /gene="argF"
                     /locus_tag="LGK42_004502"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK2660651.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          604 a          550 c          579 g          614 t
ORIGIN      
        1 ggcaaaaact ttattgcttt ataagacgct tgcgcacgta agaagagagt ggtattaaac
       61 aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc gccgcaatca
      121 cctgtaatgc gtcgcctgac gttgcaggat aatcctgcta tcgcccgcgt cattcgtcag
      181 gtatccgccg aatacggtct taccgctgat aaaggctaca ccgtcgccga tccgaatctt
      241 gacgagctgt atcaagtgta tagtcagcct ggtcatgcat attgggtggt tgagtacgag
      301 ggtgaagtgg tcggcggcgg tgggattgcg ccattagcag gtagtgaatc ggacatttgc
      361 gaactgcaaa agatgtattt tcttccggct attcgcggta aagggctggc gaaaaaactg
      421 gccttaatgg cgatggagca agcacgagag atgggcttca aacgctgcta tctggaaacg
      481 accgcttttt taaaggaagc cattgcgctt tatgagcatt tgggctttga gcatatcgac
      541 tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa
      601 aaaaatgccc gctagtgtgt gctatcgggc attttaatca attaattagt ggcgaacacc
      661 gtcatcgtct tcatcgacaa aatcttcatc gtcgccgtct tcgccattgg gatcttcaaa
      721 gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc
      781 aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat
      841 cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt
      901 aaacgcttca actgctgctt tttccagggt ttccagatcg tctgcggaaa gatggtgttc
      961 gatggtgtac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg
     1021 tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg
     1081 tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga
     1141 tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt aattcattga
     1201 ggcattaacc acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac
     1261 tcgatttcac gccagctgaa ctcaacacct tgttgcagtt agccgcgaag ctgaaagccg
     1321 ataagaaaag cggtaaagaa gaagctaaac tcactggtaa aaacatcgcg ctcatcttcg
     1381 aaaaagactc gactcgtacc cgatgctctt tcgaagttgc cgcatatgac cagggtgctc
     1441 gtgttactta tctcggccca agcggcagcc agattggtca taaagagtcg attaaagaca
     1501 ctgcccgcgt gcttggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga
     1561 ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt
     1621 ttcaccccac gcagctgctg gcggatctcc tcaccatgca ggagcatttg cccggtaaaa
     1681 cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga
     1741 tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct
     1801 ggccggaagc tgcgctggtt acggaatgcc gcgccctggc acagcaaaat ggcgggaata
     1861 ttacgctgac ggaagatgta gccaaaggag ttgaaggtgc tgactttatc tataccgatg
     1921 tatgggtgtc gatgggggaa gcaaaagaga aatgggcaga acggattgca ttactgcgtg
     1981 attatcaggt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc
     2041 actgcctgcc cgcgtttcat gacgaccaga cgacgcttgg taagaaaatg gcggaagagt
     2101 ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg
     2161 tttttgatca ggcggaaaac cgcatgcata ccatcaaagc agtgatggtc gcgacgctca
     2221 gtaaataatt gatatcgcta taaagatgcc gggaaataca aaaacatccc ggcatcacat
     2281 tattttatgc agtaatagtt caattattcc atccatattt ttctattgct atacgtaaat
     2341 cttccgg
//
LOCUS       DAFAVF010000293         2313 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFAVF010000293 DAFAVF010000000
VERSION     DAFAVF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.298"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(1..276)
                     /locus_tag="LGK42_004503"
                     /pseudo
     CDS             complement(1..276)
                     /locus_tag="LGK42_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(397..729)
                     /gene="csgC"
                     /locus_tag="LGK42_004504"
     CDS             complement(397..729)
                     /gene="csgC"
                     /locus_tag="LGK42_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBK2660652.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(788..1243)
                     /gene="csgA"
                     /locus_tag="LGK42_004505"
     CDS             complement(788..1243)
                     /gene="csgA"
                     /locus_tag="LGK42_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBK2660653.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(1284..1739)
                     /gene="csgB"
                     /locus_tag="LGK42_004506"
     CDS             complement(1284..1739)
                     /gene="csgB"
                     /locus_tag="LGK42_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBK2660654.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT          637 a          516 c          452 g          708 t
ORIGIN      
        1 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
       61 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
      121 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
      181 aatggatccc cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaagcaaact
      241 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
      301 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
      361 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
      421 ccattgttgt gataaatgaa gtgactgtcc atcagaaaca gtaacaacta ttttcacccg
      481 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
      541 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
      601 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
      661 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
      721 cgcattcata ttcttctccc gaaaaaaaac agggcttgcg ccctgtttct gtaatacaaa
      781 tgatgtatta gtactgatga gcggtcgcgt tgttaccaaa gccaacctga gtcacgttga
      841 cggaggagtt agatgcagtc tggtcaactg cagcaccgtt gccaccaccg aactgtttaa
      901 cagtcatttc agaatttttg ccgttccact gatcaagagt ggcgctgtta ccgaagccac
      961 gttgggtcag atcgattgag ctgtcatctg agccctgacc tacatctgca ccattaccac
     1021 cgccatgctg ggtaatagtc aagtcagagt tacgggcatc agtttgcaga gcaagtgcag
     1081 agttaccgcc accgtactgg taaatgttca gctctgaatt tgggccgcta ttattaccgc
     1141 caccaccgtg gttaccgccg ccgccgtact gaggaacaac acctgccaga gcgctaccgg
     1201 agaatacgat tgctgcaatt gctgctactt ttaaaagttt catgtaaaac ccccatcgga
     1261 ttgatttaaa agtcgaatgg aaattaacgt tgtgtcacgc gaatagccat ttgcgactgt
     1321 ctctgcacta caattgccgt tttttgagta ccatactgtg taatatttgc tttattacca
     1381 gaacctttct ggataatcat cgcagtatta ccataagcac cttgcgaaat actggcatcg
     1441 ttggcactgc ccgcctgatc aatatatgca aggttataat ctcctgtctg gtcaatcttt
     1501 gcccggttgc tactaccttc ttgcgcaaca accgccaaaa gttttgagcc tccctgccgt
     1561 aactgagcac tattattcgt cccagcttga ccaattatgg ctgcctgatt aaatgaagac
     1621 ttactcaatt catttaccgc gaagttatat tctgaattag ctaaatcata acctgctgcg
     1681 gctgcaatcc caggcgcacc cagtattgtt aacatcataa ataacaattt gtttttcatg
     1741 ttgtcaccct ggacctggtc gtacatttaa gaaattaaat catttcaact tggttgttaa
     1801 cgcaacctgt attttgttaa cgctgcgtta cgatggaaag tatgtctgcg gaaatatttt
     1861 taataactca cccgcgcgtt gtatttttct ttttttgttc ataccaaagt attaaaaccc
     1921 acgtaaatac gctgttatct acgcaaaaaa tattttgttt tcttttaaat ctccgttttc
     1981 cgctatcaaa aatcaccaga cagtcatttt tcttgcccgt cgctgattgc tgcgatatgt
     2041 cttgcgcaca agccgtgaca aacctcgtct ttttgcagta aaaaattgtc cacggaggtg
     2101 cggagaaaaa acaagaacgt tttacatgac gaaaggacta caccgaaata ttttttatat
     2161 gtattattag taagttatca ccatttgtat gattttttaa aattgtgcaa taaaaaccaa
     2221 atgtacaact tttctatcat ttctaaactt aataaaacct taagattaac attttaatat
     2281 aacgagttac atttagttac atgtttaaca ctt
//
LOCUS       DAFAVF010000294         2287 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFAVF010000294 DAFAVF010000000
VERSION     DAFAVF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.299"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            40..915
                     /locus_tag="LGK42_004507"
     CDS             40..915
                     /locus_tag="LGK42_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HBK2660655.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(962..1294)
                     /locus_tag="LGK42_004508"
     CDS             complement(962..1294)
                     /locus_tag="LGK42_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBK2660656.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
BASE COUNT          582 a          558 c          596 g          551 t
ORIGIN      
        1 tgttgttgtt aattcgtctc ttccagaata aggaatccca tggttaaaaa atcactgcgt
       61 cagttcacgc tgatggcgac ggcaaccgtc acgctgttgt taggaagtgt gccgctgtat
      121 gcgcaaacgg cggacgtaca gcaaaaactt gccgaattag agcggcagtc gggaggaaga
      181 ctgggtgtgg cattgattaa cacagcagat aattcgcaaa tactttatcg tgctgatgag
      241 cgctttgcga tgtgcagcac cagtaaagtg atggccgtgg ccgcggtgct gaagaaaagt
      301 gaaagcgaac cgaatctgtt aaatcagcga gttgagatca aaaaatctga cttggttaac
      361 tataatccga ttgcggaaaa gcacgtcgat gggacgatgt cactggctga gcttagcgcg
      421 gccgcgctac agtacagcga taacgtggcg atgaataagc tgatttctca cgttggcggc
      481 ccggctagcg tcaccgcgtt cgcccgacag ctgggagacg aaacgttccg tctcgaccgt
      541 accgagccga cgttaaacac cgccattccg ggcgatccgc gtgataccac ttcacctcgg
      601 gcaatggcgc aaactctgcg taatctgacg ctgggtaaag cattgggtga cagccaacgg
      661 gcgcagctgg tgacatggat gaaaggcaat accaccggtg cagcgagcat tcaggctgga
      721 ctgcctgctt cctgggttgt gggggataaa accggcagcg gtgactatgg caccaccaac
      781 gatatcgcgg tgatctggcc aaaagatcgt gcgccgctga ttctggtcac ttacttcacc
      841 cagcctcaac ctaaggcaga aagccgtcgc gatgtattag cgtcggcggc taaaatcgtc
      901 accaacggtt tgtaatagcg gaaacggaat ggggaaactc attccgtttt tgtttatcgc
      961 cttagacggc aaaagcgctg tcgcccacct gcgcttgcgc ataccaggcc ataagctccg
     1021 tggttcctgg ttctccttcc gctggcgccc agtgcgcata gtcatcggca gccacgggtt
     1081 gatagccacc gtgttttact tcaaaaatta tgccaccggt atccagcgac agcacggcat
     1141 gccaggttcc tgcggccatc tccagcaccg tacaggtttc ccccaatatc gcccgatggg
     1201 tgacggtacc ccgatcgtca aaattcagca ccacgaaacg accccttaat ggcaacagta
     1261 gttcgaaggt gtgagggtgt cggtgcgggc gcacgtaggt tcctggtaaa aacatttacg
     1321 ttatatccca aaaacacaca ggtcttaggg aggtttaaac tgtgtattaa cacctatcgt
     1381 attgacgggc gtgaaatggc agaaacagaa gtcgttccta ttgacatgcc tgactctaac
     1441 ggcgagatga cttggcaagc caaaaactac acacagtatt cgtcctactt tatgaagatt
     1501 acttgcttga aatagcttat tggcaaatgg catatacagt tattgaacca ttctgatctt
     1561 cggttgtatc gcacgcagcc gaccatgagt tattggctga tgggaatgac ctggtaagcc
     1621 atatgtaacc agttcgtgag gtgcatattc cgccgccacc tgtaacacgt tttccagttg
     1681 gacaagtagc tgttgctcca ggaaagcgcc aacctgtggc ctgtgctgta gcaatttgaa
     1741 gctcaccaga tcctaccttt ctccaggtac cggattggca ctaggccagt gccaatccgg
     1801 tcggtggtca gggggtaata aaattaatta ctcagcatgt aattggtaca aatcatccgt
     1861 agcaatgaat cactttatcg gcggtaagag tggtggttcg atctattaca agccgattca
     1921 atgtccgact ggatacatta tgactggaac acgcatgtac ggtattggtg atggtgttga
     1981 tgaggaacat gtcgatgcct actgttgccc ctttaattaa gctccagcac caggaagatt
     2041 gaggcatgtg actgatatgg tcccaccaga ctcataattt ccccaagcat aattactcac
     2101 ataccaaacg ttgttttggt cggaatatac ctgttgaaaa ccaccaaagg gtgagccatt
     2161 caggtaagtc catgagcaat atgcatggac cccgagcctt aaattacgag tgtttttagc
     2221 tatgttgtag gtctgcgttg tgaattgaat tttaggagcg ccccaggtac cggattggca
     2281 ctggcct
//
LOCUS       DAFAVF010000295         2232 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFAVF010000295 DAFAVF010000000
VERSION     DAFAVF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.300"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            32..595
                     /gene="fimA"
                     /locus_tag="LGK42_004509"
     CDS             32..595
                     /gene="fimA"
                     /locus_tag="LGK42_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBK2660657.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            956..1666
                     /locus_tag="LGK42_004510"
     CDS             956..1666
                     /locus_tag="LGK42_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBK2660658.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1708..>2232
                     /locus_tag="LGK42_004511"
     CDS             1708..>2232
                     /locus_tag="LGK42_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HBK2660659.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYV"
BASE COUNT          634 a          485 c          477 g          636 t
ORIGIN      
        1 tcagctactt aaatataaat tttggagaat tatgaaactc aaacatgttg gtatgattgt
       61 cgtttctgtg ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg atgaatcagt
      121 aacgaccact gttaatggcg gtgttattca ttttaaaggt gaagtggtaa atgccgcttg
      181 tgcaattgat tcagaatcaa tgaaccaaac ggttgagctg ggtcaggttc gttcttctcg
      241 cctggctaaa gcgggtgacc tcagctccgc cgttggcttc aatatcaagc tgaatgattg
      301 tgataccaat gtttccagta atgcagctgt tgcattcctg ggtactactg tcaccagtaa
      361 tgacgatacg ttagcgctgc agagttcagc ggcaggctct gcccaaaatg tcggtattca
      421 aattttggac cgtacgggtg aggtattaat acttgatggg gccactttta gtgctaaaac
      481 cgacttgatt gatggcacga atatactgcc attccaggct cgttatattg ctctcgggca
      541 gtccgtagct ggtactgcaa acgctgatgc gaccttcaaa gttcaatatc tataattcaa
      601 tcttaaggtg gctatacgtc atctgaaatt gaaacagggt gtgtgtggca tggctacgca
      661 cacccgagca atttaccaat attcatctca tttttaatga gaaatggttt gtatttggag
      721 cgctatgaaa aatcacataa tatctaaact atcaataaac aacagtagtt aaacttactg
      781 ttatttaaaa tatttacttt gtagcaaggt tttcctatta aaagagaacc aacatttatt
      841 acaaacgcat taagtctgga aacccggcga ctcgccaatg tattagcaac attaatcgat
      901 tgtctgatgt aacggttttc ccgcttatcc tcttaacaca tggagaatgc atcagatgca
      961 aactacgcgt accccctact ctatttcctt tatggcgaca gtgttacttt tattgctctt
     1021 tgcttgccat agcaccgttg ccaacgcagc tgtcgcactg ggtgcgacgc gggtaattta
     1081 tccggcaaac cagaaacagg ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta
     1141 tctcattcag tcatggatag aaaatgcagg tgaccaaaaa gacacccagt ttgtcattac
     1201 cccaccgctt ttttccatgc aaggtaaaaa agagaatacg ctgcggataa tcaacgctac
     1261 taatcatcag ttaccaggcg accgtgaaag cctgttctgg gtgaatgtta aagctattcc
     1321 tgctatggaa aaagaccaga agaatgagaa tacattacag ctggcaatta ttagccgcat
     1381 aaaaatgttt tatcgcccca cgaacctggc aatggcacct gaagaagcgc ctgcaatgct
     1441 tcggtttcgt cgcagcggta gcaaactcac gcttattaat ccaacgccct atttcattac
     1501 cgtaacaaat atgaaagcag gaaatagcaa tctcccaaac accatggtgc cgccaaaagg
     1561 tgaagtatcg gtggatatca cgcatgctgc aaccggggat atcagttttc agacgatcaa
     1621 tgattacggt gcattaactc cgcgtattaa ggcaaccatg cagtaagcca gtgacctaat
     1681 taataataaa ctcaatcgga attaataatg acagccattc gtgccgcatt taaggcatat
     1741 cgtatgcatc aggtgttaat aatgcctcgc tttgcacggc ttaccattgc ccttggctta
     1801 gcaactgcgg tatttcccgt tgacgcagag tattatttta atccacggtt tttaagcaat
     1861 gatcttgctg aatctgtcga tttatccgcc tttactaagg gacgcgaggc accacctggt
     1921 acatatcggg tcgacattta tttgaacgat gaatttatga ctagtcggga tattactttt
     1981 attgcagatg ataataatgc agaactcatc ccatgcctga gtacagatct tcttgtcagt
     2041 cttggtataa aaaaatcagc gttattagac aataaagaac attcggcaga aaaacatgtg
     2101 ccggacaaca gcgcatgcac accacttcag gatcgtttgg ccgacgcatc aactgagttt
     2161 gatgttggtc agcaacattt gtcactctcc gttccgcaaa tttatgttgg cagaatggct
     2221 cgcggctatg tt
//
LOCUS       DAFAVF010000296         2226 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFAVF010000296 DAFAVF010000000
VERSION     DAFAVF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.301"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            14..157
                     /locus_tag="LGK42_004512"
     CDS             14..157
                     /locus_tag="LGK42_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBK2660660.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            170..379
                     /gene="cspE"
                     /locus_tag="LGK42_004513"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             170..379
                     /gene="cspE"
                     /locus_tag="LGK42_004513"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBK2660661.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            545..1354
                     /gene="rlmA"
                     /locus_tag="LGK42_004514"
     CDS             545..1354
                     /gene="rlmA"
                     /locus_tag="LGK42_004514"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBK2660662.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1351..1917)
                     /gene="mntP"
                     /locus_tag="LGK42_004515"
     CDS             complement(1351..1917)
                     /gene="mntP"
                     /locus_tag="LGK42_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBK2660663.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          577 a          551 c          541 g          557 t
ORIGIN      
        1 tacagttctg tttatgtgtg gcattttcag taaagaagtc ctgagtaaac acgttgacgt
       61 tgaataccgc ttctctgccg agccttatat tggtgcctca tgcagtaatg tgtcagtttt
      121 atctatgtta tgcctgcggg cgaagaaaac aatctaagga atttttcaaa tggcaaagat
      181 taaaggtcag gttaagtggt tcaacgagtc taaaggtttt ggcttcatta ctccggctga
      241 tggcagcaaa gacgtgttcg tacacttctc cgctatccag ggtaatggct tcaaaactct
      301 ggctgaaggt cagaacgttg agttcgaaat tcaggacggc cagaaaggtc cggcagctgt
      361 taacgtaaca gctatctgat cgaatccact gatctgaagt gtgaatacgc ttcaatctcg
      421 ctataaagcc tcgtcgaatg cgaggctttt tactatgctt tatcttcgct cctggcgttc
      481 ggatatttgc ccgccgcgtg attcgcgtta cacttgcggc ctttagtatc ctgccggagt
      541 tgtcatgtcg ttttcctgtc cactttgcca tcagcctctt tcgcgtgaaa aaaacagcta
      601 tatctgtccc cagcgacatc agtttgatat ggcgaaagaa gggtatgtca atctgctgcc
      661 cgttcagcat aaacggtctc gtgatccggg cgacagcgcg gaaatgatgc aagcacgccg
      721 cgcattctta gatgccggac attatcagcc gctgcgtgat gcaattgtcg cccaactgcg
      781 ggaacggctt gatgaaaagg ccacggcagt gctggatatt ggctgtggtg aagggtatta
      841 cacacacgca tttgccgatg cgttgcccga aatcaccacg tttggcctgg atgtctcgaa
      901 ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc actctttgtg tcgcttccag
      961 ccaccgtttg ccgttttccg ataccagtat ggacgccata atacgtattt acgcgccgtg
     1021 taaagcagaa gaattagcac gagtagtgaa gcccggcggc tgggtcatta ctgccacgcc
     1081 gggaccgcga catttgatgg agctgaaggg gctgatttac aatgaagtac atcttcatgc
     1141 acctcatgca gaacaactgg aaggttttac attacagcag agtgatgagt tgtgttatcc
     1201 gatgcgtctt cgcggtgatg aagccgtcgc attattgcag atgacgccgt ttgcctggcg
     1261 tgcgaagcca gaagtctggc aaacactggc agcaaaagaa gtgttcgact gccagacgga
     1321 ctttaatatt cacctctggc agcgttctta ttaaccgtgg aagtgcgtcc agaggatctg
     1381 gacgccgatg ccgatcagta ccagcccgcc gagaatttcc gcttttttcc caataattga
     1441 gccgataaag cgaccaacca tcatccctaa tgtcgacata atcaaggttg cacaaccaat
     1501 ggccaatgcg gtcgcgataa tgttgacctg caggaaagca agaccaacac ccacagccat
     1561 ggcatccagg ctggtggcaa tcgcggtggt taccagtagc cagaaaccgt gccgacggcg
     1621 cggctcttca tcttcatcat ctgcgccacg aaaaccctca ataatcattc gcccgccgag
     1681 gaatatcagc agcacaaacg caatccagtg attccattca aggacaaacc ggctggctaa
     1741 catgcccatt ccccagccga tcagcggcgt cagggtttcg acggcaccaa aaataaggcc
     1801 ggttcgcaat gcttcagaaa atttcggttt atggagggtg gcacctttac cgattgatgc
     1861 agcaaatgca tccatcgaca taccaaacgc aagaagaaca gtagcagtga tattcatgac
     1921 aacgtcctga ccggggtatc cataaaacac atcatcgccc ccagcaaaca tgcatacatg
     1981 aacaacaagc cgcgatggtt ttcgcgttca tgcaggcata gcgattgatg tgcatatggt
     2041 ctcgcctgac tgaaagtctt cagtccgtac gtgccacgtt ttgcaacgag tatgttgaca
     2101 cgtacatttc cggaatctgg aaatcagcta ctccccaaat gagggcgcaa ccttaacata
     2161 ttttgagaat ataaaacaac tgttggaata tattatttca ttgctttatt gataacgatt
     2221 tttatt
//
LOCUS       DAFAVF010000297         2189 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFAVF010000297 DAFAVF010000000
VERSION     DAFAVF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.302"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..141
                     /locus_tag="LGK42_004516"
     CDS             <1..141
                     /locus_tag="LGK42_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBK2660664.1"
                     /translation="YRKNLATAGVFRWITNIHENKRYYYTFDNSLLFTENIQNTTQIF
                     PH"
     gene            complement(493..645)
                     /locus_tag="LGK42_004517"
     CDS             complement(493..645)
                     /locus_tag="LGK42_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660665.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(633..1100)
                     /locus_tag="LGK42_004518"
     CDS             complement(633..1100)
                     /locus_tag="LGK42_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBK2660666.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDNKASEL
                     EKANATITDMQQRQRDADALDDKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1097..1594)
                     /gene="rrrD"
                     /locus_tag="LGK42_004519"
     CDS             complement(1097..1594)
                     /gene="rrrD"
                     /locus_tag="LGK42_004519"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBK2660667.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWAYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1594..1809)
                     /gene="essD"
                     /locus_tag="LGK42_004520"
     CDS             complement(1594..1809)
                     /gene="essD"
                     /locus_tag="LGK42_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBK2660668.1"
                     /translation="MKSMDKLTTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(1997..>2189)
                     /locus_tag="LGK42_004521"
     CDS             complement(1997..>2189)
                     /locus_tag="LGK42_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660669.1"
                     /translation="KLILEERMVMAERLLSYNLYSVGKVAEICGYENTSYFVSVFRRY
                     FGVPPHQYSSRFFLEKDMM"
BASE COUNT          590 a          529 c          476 g          594 t
ORIGIN      
        1 tacaggaaaa atcttgctac agcaggggtt ttcagatgga ttacaaatat ccacgaaaac
       61 aaaaggtatt actatacctt tgataattca ttactcttta ctgagaacat tcagaacact
      121 acacaaatat ttccgcacta aatcataacg tccggtttct tccgtgccag aaccggactc
      181 gctggcatga tgaaatatgt gtacccggta accccggtgt gcatcgtttt tgattattcc
      241 cgcacactcg cgcagaagga gttccccgtc gggctacggt ctctgttaat acgggaatac
      301 ggcgacgata cagcgcatga tgtgtcaggc ttgaatacct ttatccttta aaagggatat
      361 cagttaagtt atcccgtgta gggtataaac cattatcaaa gccactctgt aggaagtggc
      421 ttttgtaatg gcaataaaaa gccccgcgaa tgcgaggcta aatcctggta tttgtaatga
      481 ctggttctta tctcaacgca gccccttacc gcgcgcaaaa tgctcaatat caagcatcag
      541 caatgagatg tttaatctgg attcactcca gaagtgagca ccaccctgtc tacagagcca
      601 gatgtgaagg atgatgagta aaattatcgc tatcatcgaa ggcattgcgt cctgatgtat
      661 tcctgaagcg ttctcagtgc tgtttggtcg cggataattc cgtcccggat accgagaacg
      721 tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc ggaggcgctg
      781 gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg accaccatta
      841 tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc cttcgtgtat
      901 ttatcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat ggtggcgttc
      961 gccttctcca gttcactggc cttgttatcg cgctgttctt tgtaggcgat tgcattatca
     1021 cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag agcggagata
     1081 atcgcggtta ccctgctcat tgttgccccc acaaacagac ctcacgctca atctcacgac
     1141 gagtcatcag gcctttccat tgcttaccgc cagcgtatgc ccagcgacgt agctggtcac
     1201 atgcgccctt gatatcgccc tggtttattt tgcgaagaag agtagatgtt ctgaaattgc
     1261 ctgcgcccac gttatagacg aacgagtaaa gagcgccgcg cgttgtttcc ggtatatcga
     1321 ctttgatgta cgggttaatt tgtctggcta ccgtggcaag gtctttattc aggagggctt
     1381 tgcattctgc ttcggtatac gttttaccgg gaatgatgtc ttttcctgta tgcccgtgac
     1441 atacagtcca tacgccaacg atatctttgt atggtatgta gctgacacct tccagaccat
     1501 cgttaccact tgggccagtg attaacactg atgctatagc aattgctccg ccaccaatag
     1561 cagcagcaac ggcttttcgt aatgatggag gcattattca cctctcgcag ccttgcgctt
     1621 atcttcttta atcttgaaat aaaggtttgt caggtacgtc agcaggccaa ataccaagct
     1681 acccagcaca cctattgctg cccactgtga gggcgtgact ttatcgagca gctgtaaaaa
     1741 ccagtacccg gcactacctg ctgaggtgcc gtaggcaatg cccgttgtta acttatccat
     1801 ggatttcata gcctcacctc cgcaaataac ggatggtgta cacggttcgg aacgaagagg
     1861 aaaggtatag aagttacatt agcgtaaggc ttgaacatct attcaaaaag aaaaacgccg
     1921 cgattattct ggcgtagctg aaagcatcat acaattatca aatacgaaaa ttacaaaatc
     1981 attaaaacgc atcacgttac atcatgtctt tttctaaaaa aaatcttgat gaatattgat
     2041 ggggaggaac accaaaatac cttctgaaaa cacttacaaa atatgacgtg ttttcataac
     2101 cgcatatctc agcaactttt ccaacagaat ataaattgta gcttaataac ctttccgcca
     2161 tcaccattcg ctcttcaaga attaactta
//
LOCUS       DAFAVF010000298         2186 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFAVF010000298 DAFAVF010000000
VERSION     DAFAVF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.303"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            1371..1940
                     /locus_tag="LGK42_004522"
     CDS             1371..1940
                     /locus_tag="LGK42_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBK2660670.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEYTKAHCTGERNFGCSRS"
BASE COUNT          546 a          552 c          573 g          515 t
ORIGIN      
        1 tctgataatt gcagagttgt tttcggaggt ggttacagtg agtcagcccg ttcctggcga
       61 ggaggagact gatatcaccg atgggataag gctgtctgat tggtattata aatataagaa
      121 gtcatcagag agtaacgtaa aggatttgcc agagtaagct aagagagtaa atctctccgg
      181 taacagagcg ctgtgaagca ttctgtatca gagtgcaaat acatgaacgc tgatgcgtaa
      241 aatcagcagt gacacgccag ctccccctgt gcccattcct gtataagaca tgaactgagc
      301 gtattaatgc tcctgctctg agactgagcc ggtgagtgag gggctgagaa atccctcttg
      361 acacagctct aaaccagaaa tacttccccg gttgttgcgg tgcgtccgga tccccgataa
      421 cggaacacta aggctgtatt gcgggacacc gggtaagtgg cagcctgtaa tgtgttctgt
      481 caacgccaga aaaaatgatt tttcacacgc gttatagact cactcctgtg gacctggaag
      541 cccgtaactc tgcggttgcc caccgactga cggaactgac cgcaaacttt acgatcgtgt
      601 tcctgcttcc ttctggtcac ctgaactttt agccagcgct acgataagtc cggtgttatc
      661 tgcaccggag gaccgggaac attaagcggt atagcgacaa caaagaattc gaaatcaaaa
      721 gggaatggtg ccggaggcgt gggcggtaat aggctgattt tcaggtatta ccggcttaca
      781 acgtgtggtt gatattgagg ggctgtgagt ttcgcgtatt tgtcagaaac ccagatgagt
      841 tttcctttat gggacgaaat aaggcagcgg caatacctgg aaaaatttaa tatggtatga
      901 aggagaacct gtcggttacg cttaatatac acggtacact gtcagctcag gggatacatg
      961 aaccgttatc tcaccagcta cacagtatac acacactgac gctgacagta gtcagcagga
     1021 ccacaataat cagcgccagc agtatggtaa cgatacggtc tgtattccgg tagtagccga
     1081 aggggtcgac agaaccacaa tacgggcaac acactgtatt atgagtaatc gcgtttccac
     1141 agtccctgca cttctttttg cgtatcacca tcactccctc ctcacagcct tacccgtaac
     1201 cgatttttta cattaaaaaa acaacgctga aaattcaacc gtcagtccgg agacgaccgt
     1261 tcgggttatc acagagtgcc tgagacagtg tcctgccgga ggtcacttca gggactctgc
     1321 gcatttttta cgacgtggct attccgtatg aaccatacgg agatttaacc atgacctaca
     1381 aatacaaccc cttctggcag caacgtattc gtgagacggt gcggcacgca ctgaatgttc
     1441 atccccgcct gacggcattg cgggttgacc tgcgtctccc ggatgtaccg gcagcaacgg
     1501 acgcagctgt gatatcccgc ttcatcaatg ccctgaaagc ccgaatcgac gcttaccaga
     1561 aacgtaagca tcgggaaggt aaacgcgtgc atcccacaac cctgcattac gtctgggccc
     1621 gggagtttgg ggagtgcaaa ggtaaaaaac actatcacct gatgctgctg gtcaaccggg
     1681 atacctggtg tcgtgccggt gattaccgcg ctccggagtc tctggccggg atgattaaac
     1741 aggcgtggtg cagtgccctg ggagtggatg tcgggtgcca tgccacgctg gtgcattttc
     1801 cggcctggcc ggcggtgtgg ctggcgcgta atgatgacac cggctttcag caagtgctgg
     1861 aacgtgctga ctatctggcg aaggagtata ccaaagctca ctgcaccggt gagcgcaatt
     1921 ttggctgtag ccggagttga gcacgactgg cgcgccaata tccggcaaac caaaagtatg
     1981 ggttgttgtc tggcggcaac gatgtatcca ctcgcccact gactgttttt tacgtcagtg
     2041 ttccattgtg tgacaaccac agtgctgtaa cagcaaccag cctgctttac ccaaactcca
     2101 gtccagattt taactgaacc gccattcaca ccctgaccac gctgcccgta ccgggacggc
     2161 tgttgcctgc gtgtcgtctg gcggct
//
LOCUS       DAFAVF010000299         2127 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFAVF010000299 DAFAVF010000000
VERSION     DAFAVF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.304"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            268..849
                     /gene="mdaB"
                     /locus_tag="LGK42_004523"
     CDS             268..849
                     /gene="mdaB"
                     /locus_tag="LGK42_004523"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBK2660671.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYVVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            880..1194
                     /locus_tag="LGK42_004524"
     CDS             880..1194
                     /locus_tag="LGK42_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK2660672.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1238..>2127)
                     /locus_tag="LGK42_004525"
     CDS             complement(1238..>2127)
                     /locus_tag="LGK42_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK2660673.1"
                     /translation="FVMTKIAMFAVTIIVPLSAMYLGKNNVQLGYQIAMGTMGILATC
                     LCLYCFFSVRERIYHPKPSLGMAAQFRLLIKNDQWLILGAVIAIIMFGGIIRNSVAAY
                     YAKYYLSGGDALISPFLTSGVIASVLAMIACTWLTRLYDKIKIFRYTQLLAFVVGGAM
                     YFVQPNSIVLAFTFYIVVTFLTDIQLPIYWASIAESVDYGEMKTGQRVSGLAFGGILF
                     FQKFGMGLAGGFIGIALAYLDYQPGVEQTPQALWGICLLMTIIPAILNLLTGLIMRFY
                     LINNEYYEQIKARLQTAEE"
BASE COUNT          625 a          496 c          472 g          534 t
ORIGIN      
        1 atattttcat cttttactta ttcaaagcca tattttcaat cccaaaaaaa accgaaccaa
       61 acgtgtaaat tattccggtg cgagtaagaa ttatgaggaa tggctatcag tattgtcatt
      121 ttcagaaaat atttatcctg catcggtgag tcagagctag atcagacttt tgctaaattc
      181 gcaaaagact ttgcacattt tgctaatttc accgtaccgc tctgtgacgt actatagtcg
      241 acaaacgtct caccttgagg ttaaaaaatg agcaacatcc tgattatcaa cggcgcgaaa
      301 aaattcgccc actccaatgg tcaactgaac gacaccctga ccgaagtcgc ggatggcaca
      361 ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg acagcgacta cgttgtcaaa
      421 gcggaagtac aaaactttct ctgggctgat gtggtgatct ggcagatgcc aggctggtgg
      481 atgggcgcgc cgtggacagt gaaaaaatat attgatgatg tattcaccga aggtcatgga
      541 acgctgtatg ccagcgatgg tcgtacccgc aaagatccgt cgaaaaaata cggttccggc
      601 ggcctggtac agggcaaaaa atatatgctt tctctaacct ggaacgcacc aatggaagcc
      661 ttcaccgaaa aagatcagtt cttccacggc gttggcgttg acggtgtgta tctgccgttc
      721 cataaagcaa accaattcct cggtatggaa ccgctgccga catttatcgc taatgacgtg
      781 ataaaaatgc ctgatgttcc ccgctatact gaagaatatc gcaagcatct tgtggaaatt
      841 tttggttaac tagagctcag gctttagaag gagttaacca tgcttaccgt aatcgcagaa
      901 atccgtactc gtcctggtca acatcaccgt caggcggtat tggatcagtt tgctaaaatc
      961 gttccaaccg tactgaaaga agaaggttgc cacggctatg cgccaatggt ggattgcgct
     1021 gctggcgtga gtttccagtc tatggcaccg gattctatcg tgatgattga gcagtgggaa
     1081 agcatcgcgc atcttgaagc gcatctgcaa accccgcaca tgaaggcgta cagcgaagcc
     1141 gtaaaaggcg acgtgctgga gatgaatatt cgtattctgc agccagggat ttaatatttc
     1201 tgaaaagagg ccggtgctgc ttgccggcca tttattttca ctcttccgcc gtttgtaacc
     1261 gtgcttttat ctgttcgtaa tattcattat taatcagata aaatctcatg atgagtccgg
     1321 taagcaggtt aagtatcgcg ggaatgatcg tcattaacag gcagatcccc cacagcgcct
     1381 gtggtgtttg ttctacgcct ggttgataat ccagataggc taaagcgatt ccgataaagc
     1441 cccctgccag ccccataccg aacttttgga aaaagagaat cccgccaaat gccaggccag
     1501 aaacacgttg ccccgttttc atttcaccgt aatccacgga ctcagcaatg gatgcccaat
     1561 aaatgggaag ctggatatcc gtcaggaaag tcaccacaat ataaaatgta aaagctagca
     1621 ctatagaatt tggttgcacg aaatacatcg caccacccac cacgaacgcc agtaattggg
     1681 tataacgaaa tatttttatt ttgtcataga gacgagtaag ccaggtgcaa gcgatcatag
     1741 ccagaactga ggcgatcacg cctgaagtta aaaacggtga aattaaagca tccccaccac
     1801 tcaggtaata ttttgcataa tatgcagcga cagagttacg aataatcccg ccaaacataa
     1861 tgatcgcaat tacagcgccc aaaatgagcc attgatcatt tttgatcagt aaccgaaact
     1921 gcgcggccat accgagactg ggttttggat ggtagatgcg ctctctcacg ctaaaaaaac
     1981 aatacaggca taaacacgtt gccagaatac ccattgtccc cattgctatt tgataaccca
     2041 gttgtacgtt attcttccca agatacattg cagataaagg aacaataatt gttacggcga
     2101 acatggcaat ttttgtcata acaaaac
//
LOCUS       DAFAVF010000300         2113 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFAVF010000300 DAFAVF010000000
VERSION     DAFAVF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.305"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..931)
                     /locus_tag="LGK42_004526"
     CDS             complement(<1..931)
                     /locus_tag="LGK42_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HBK2660674.1"
                     /translation="MIKKPVIGISGCLAGSAVRFDGGHKRADFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSTQGNIRMCFSHDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FVERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDKLMAILRKPASRKNHTNVLMHIQGYFSNYLSTRQRKELSEVILNYRSG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPD"
     gene            1124..1648
                     /locus_tag="LGK42_004527"
     CDS             1124..1648
                     /locus_tag="LGK42_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBK2660675.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENRFERGLEQVSATYGKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTPTIPDKVKADYVRTAQ
                     KLGFNVNELLWVKQ"
     gene            complement(1804..>2113)
                     /locus_tag="LGK42_004528"
     CDS             complement(1804..>2113)
                     /locus_tag="LGK42_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBK2660676.1"
                     /translation="WSSIAAGFKGLITSKVKSRPQCCDDDAMIICGCMARLKKNNSDL
                     HDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKAEGVVEGMLMVLDLRLEMDADCSK
                     "
BASE COUNT          641 a          510 c          393 g          569 t
ORIGIN      
        1 cgtccggata gggatcgaag taattctgtg taagcaagta atcattagga tactcaccca
       61 gataatgctt cagcagagtc aacggcgcaa gaagaggtaa tgtgccagaa cgatagttaa
      121 gtataacctc gctcaactct ttacgctggc gtgtacttaa gtagttacta aaatacccct
      181 gtatatgcat cagcacattc gtgtgatttt tacgtgatgc aggttttctg agaatcgcca
      241 tcagcttatc acgatacacc tcaaagtatg attcaaggtc cgcccactcg tgtattgcag
      301 ccacaaatgg tcccatatct ttatagcctg cctgactatg cgccaacaac tgaagcttat
      361 aacgactatg aaaagctaat aactctcttc ttgataattt ctccttgtaa aggtgattga
      421 gctcatgcaa agcaaaaact ctttcaacaa aattctcacg aagcactgga tcatgtaatc
      481 gcccatcctc ttcaaccggt agccaggaaa acttttccat caaagtgctc gtaaatagtc
      541 ccactccatc tttacgacct cgattaccat tttcatcata gacacgcacg cgctccatgc
      601 cacagctggg agatttagca caaaccacaa accccgatac atcctttaat ttgtccatat
      661 aagaacgact aaactctgtc attctctctg tcacatcctc attctggtcg tggctgaaac
      721 acatccgtat atttccttgc gtcgagcgca caagacgtag agcaggacgc ggaactggca
      781 gccctatagc catttccgga catactggtc tgaatgttac ccattccact aatttgtcca
      841 ttaaaaagtc agctcttttg tgaccaccat caaaacgaac agcagaaccg gccaaacaac
      901 cgctgattcc aatcacaggt ttttttatca tattctcccc cttgactaat tcattaacac
      961 ataaactgtg tagtgcacgg aataaattgc ctttctggcg tcatcactga caatttttct
     1021 gttatggact attcctaata tagtatgaaa gttctttaag tgatcggtcg taatcatcta
     1081 tctttcatac ttactctcaa ctatcaaaag tacaggattt attatgaagt tatggcctgt
     1141 gttgactggc attgcactct ctttcactct tatagcatgt aaggccccga caccacctaa
     1201 aggtgtgcag ccgattacaa attttgacgc caaccgctac ctcggaaaat ggtatgaaat
     1261 agctcgcctc gagaaccggt tcgaacgtgg tctggaacag gtcagcgcta cttatggaaa
     1321 acggaacgac ggagggattc gtgtacttaa ccgtggatac gatccaacga aaaataaatg
     1381 gagcgagagc gaaggtaaag catactttac tggagatact aaaactgcag cattgaaggt
     1441 ttcgtttttt ggccccttct atggtggcta taatgtaatc aaactggatg atgagtataa
     1501 gtatgctctt gtcagtggtc cgaacagaga atacctatgg attctggcaa ggaccccaac
     1561 tattccagat aaagtaaaag cagactatgt gcgaaccgct caaaagttgg gattcaatgt
     1621 caatgaatta ttatgggtta aacaataaaa tccctacccg aaataatact tattagaaaa
     1681 aaaccagcct ttggggaggc tggctaaatc aggaaacaag ctgttatatg ataataacta
     1741 cgttgcgatt ccaacattta aaatgttaga ctaatgacaa tcagacagca acttttcctt
     1801 taattatttc gaacaatcag catccatctc caatcggaga tccaacacca tcagcatacc
     1861 ctccactacg ccctcagctt tctgaagcat cctgccaacc caacaatcag atcgcccatg
     1921 cttacgtgca agcgccataa aagtcatgcc gccgacataa tagtccacca ataaatcatg
     1981 caaatcgctg ttgttctttt tcagacgggc catgcacccg caaatgatca tcgcgtcatc
     2041 gtcacaacat tgtgggcgag attttacttt tgaagtaatt aatcccttaa aaccggcggc
     2101 aatggacgac cag
//
LOCUS       DAFAVF010000301         2078 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFAVF010000301 DAFAVF010000000
VERSION     DAFAVF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.306"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            213..1367
                     /locus_tag="LGK42_004529"
     CDS             213..1367
                     /locus_tag="LGK42_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK2660677.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1518..>2078
                     /locus_tag="LGK42_004530"
     CDS             1518..>2078
                     /locus_tag="LGK42_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK2660678.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPF"
BASE COUNT          574 a          506 c          460 g          538 t
ORIGIN      
        1 ttgagcactt ttctttcacc caatgggtga aagctgaatt tcgaaggaat gcatatttat
       61 caaggctttg attatgcttt ttgaagtact gacgtagaat ctaggttttt atattcgtcc
      121 atatcgacgc tgatgatgat ttccaggtac cggattggca cgcttgcaga gaatgattgt
      181 tggcattatt ttttgcctct tttgaggtta gcatgccgtc tccacgcatc cgtaaaatgt
      241 ccctgtcacg cgcactggat aagtacctga aaacagtttc tgttcacaag aaagggcatc
      301 aacaggagtt ttaccggagc aatgttatca agcgatatcc cattgccctt cggaatatgg
      361 acgaaataac aaccgttgat attgctacat acagagacgt tcgtttagca gaaatcaacc
      421 cccgaacggg taaacccatt acaggtaata ctgtacgtct tgaactcgcc cttctgtcat
      481 ctctgttcaa tattgctcgt gttgaatggg gaacctgtcg tactaacccg gttgaactgg
      541 ttcgcaagcc gaaagtatcc tcgggacgag atcgccggct aacgtcttca gaagaacgtc
      601 gcctttctcg ctatttccgc gaaaaaaatc tgatgttgta tgtcattttc catcttgccc
      661 ttgagacagc catgcggcag ggcgaaatac tggccttacg ttgggagcac attgatttgc
      721 gccacggtgt ggctcattta cctgaaacca aaaacggtca ctcacgggat gttcctctgt
      781 ccagacgtgc ccgtaacttt cttcagatga tgcccgttaa tctccacggc aatgtttttg
      841 attacaccgc atccggcttt aaaaatgcct ggagaatagc cacacaacga cttcgcatcg
      901 aggacctgca ttttcacgat ctacggcatg aagcaataag ccgcttcttc gaactgggta
      961 gcctgaatgt aatggagatt gctgcaatat caggacatcg ttccatgaat atgctgaaac
     1021 ggtatactca tcttcgtgca tggcaactgg tcagtaagct tgatgcccgc cggcggcaga
     1081 cacaaaaagt ggcagcatgg tttgtgccgt atcctgccca tatcacgacc atcgatgaag
     1141 aaaatgggca gaaagcgcat cgtattgaga tcggtgattt tgataacctt cacgtcactg
     1201 ccacaacgaa agaggaagca gttcaccgcg ccagtgaggt tttgttgcgt acactggcca
     1261 ttgcagcaca gaaaggcgaa cgtgtcccat ctcccggagc gttacctgtt aacgaccctg
     1321 actacattat gatttgccct ctgaacccgg gcagcacccc gctgtaaaca tcattcaaaa
     1381 cacatacaga aaggcgagtt gcaacaactc gccaaacaca ttcgatctgc atgatcgaaa
     1441 ttattcgttt caccagaaaa aacaccgaag atacaataac cacatgagct gaaaagactt
     1501 tttcagtcgg aggaacaatg agactgaata cgaccggtat tgcggcccga atgatgctct
     1561 cgctggacaa aaaagcaatc ggcgaaaaac tcatcaacgg tcgtcagata aacccgacac
     1621 ctctgcaggt ccgttacccc caaggggtaa gagaagttct ggggataatg agcgaacaat
     1681 tagccatatc caccgctgat ttaacccgaa tcctgcttga ggatgctctt cataatatgt
     1741 ttatgcctgc ggataacacc acaggaaaca taataagcag aattgaatac atcatgctgt
     1801 cgcatgatat aaatgcgcca ttactggcaa cattgctgtc gccatggaat atccgctcca
     1861 cggttataca ggacccggcc agacttgcag actatctttc agctgacgct ctcgaacatc
     1921 ttgctgaatg ttttaatctc aatccggact ggctgaatgg ccatgaaaac tatccgatag
     1981 cgttgtccgg tgaatggcca gatactgctg ataatttcag aatgcttatt agtgatagca
     2041 gcaacactga agttattttc tggcatagtt tccccttc
//
LOCUS       DAFAVF010000302         2075 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFAVF010000302 DAFAVF010000000
VERSION     DAFAVF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.307"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            285..1808
                     /locus_tag="LGK42_004531"
     CDS             285..1808
                     /locus_tag="LGK42_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2660679.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          550 a          448 c          450 g          627 t
ORIGIN      
        1 atgcaaagtg agcatttcag gcgttatgct ttcttattat gtccgcaata tcaggtgtca
       61 agaatggaga gttctcgctc tccattcttg acgcctgata tcccgcctaa cttattttgt
      121 actgcctaca ttatgctcaa taattaatgg gttagatcaa gatggagaaa attttatctt
      181 cggcgtggat atttcttatg ctctctacca tgctctctgt agatgccagc gcgagcgaat
      241 gaatggcatt ttcatgtatt aaaccctttg gccgggacaa gcaaatgcgc aatacactca
      301 tacccatcct cgttgcgata tgcttgttta ttaccggtgt cgctattttg aacatccagc
      361 tctggtattc cgcaaaagca gagtacctgg cgggagcgag atatgccgcc aacaatatca
      421 atcatatact tgaagaagcg tcacaagcga ctcaaacagc ggttaacatt gccgggaagg
      481 aatgcaacct cgaggagcaa tatcagcttg gcactgaagc agctctgaaa cctcacctgc
      541 gcacaatcat cattctcaaa cagggaatag tctggtgtac atccctgcct gggaatcggg
      601 tcctgttgtc tcgtattcct gttttcccgg acagtaattt actgttggct ccagcaatcg
      661 acaccgttaa tagattacct atcctgctct atcagaacca atttgcagat acgcgcattt
      721 tggttacgat aagcgatcag catattcgtg gggcacttaa tgtacccttg aaaggggtaa
      781 ggtatgtatt acgcgtggcg gatgacatta ttggaccaac gggtgatgtg atgacgctta
      841 atggacatta tccctatacc gagaaggttc actccacaaa atatcatttc actattatct
      901 ttaacccgcc gccactcttt agcttctaca gacttatcga taaaggcttt gggattttaa
      961 tatttattct gttaatcgcc tgcgccgctg ccttcctgct tgatagatat ttcaataaaa
     1021 gcgcaacgcc tgaagagatc ctgcgacggg caataaataa tggggagatc gtcccttttt
     1081 accaacctgt ggtaaatggt cgggaaggga cattgcgggg agttgaggtg ttagcccgct
     1141 ggaaacaacc tcacggtgga tatatatcac ccgcggcatt tattccactt gctgaaaaat
     1201 ccggattaat cgttccgctt acgcaaagcc tgatgaatca ggttgccaga cagatgaacg
     1261 ctatcgcgag taaattgccg gaaggttttc atattggaat taattttagc gcttcgcata
     1321 ttatttcgcc gacgtttgtc gacgagtgtt taaatttccg tgacagtttt acccgccgcg
     1381 atttaaacct tgttctggaa gtcaccgagc gtgagccatt gaatgttgat gaaagtctgg
     1441 ttcagcggtt gaacattctg catgaaaatg gttttgtcat cgcgctggat gatttcggta
     1501 ctggctactc agggctttct tatcttcatg acctgcatat tgattatatc aaaattgatc
     1561 acagtttcgt tggccgcgta aacgcagacc cagaatcaac ccgaattctg gattgtgtat
     1621 tggatctggc gcgtaaactt tcgatcagta tcgtcgctga aggtgtcgaa acgaaagaac
     1681 aacttgacta tctgaaccaa aataatatca catttcagca gggttattat ttctataaac
     1741 ctgttacata catcgacctg gtcaaaatta tcctttctaa accgaaggtg aaggttgtgg
     1801 ttgagtgaaa attgatcagt aaggccatag tgcggtgtaa ttatagacag ctaattagct
     1861 cgttgcctct tgttactatt gttcattatt ttgtttgcta taattgtttg aaagttttga
     1921 caggattgcc attagtagca tgaacaatag taataatctg gattatttca ctctctatat
     1981 catcttttcc attgcattta tgctgatcac cctcctggtc atccttattg caaaacccag
     2041 taccgggctg ggagaagtgc ttgtgacgat aaatt
//
LOCUS       DAFAVF010000303         1977 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFAVF010000303 DAFAVF010000000
VERSION     DAFAVF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.310"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(242..391)
                     /locus_tag="LGK42_004532"
                     /pseudo
     CDS             complement(242..391)
                     /locus_tag="LGK42_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(569..1762)
                     /locus_tag="LGK42_004533"
     CDS             complement(569..1762)
                     /locus_tag="LGK42_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK2660680.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDHLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALAGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VTRLS"
BASE COUNT          490 a          468 c          460 g          559 t
ORIGIN      
        1 attcctaaaa tgagccacaa atagacatta gcattgagcc tctacaatgt ttgtgcgcac
       61 aatctatctt atgttaaata acattgaatg ttaacttact tcatgaagct tactgcttct
      121 atttccactt ctttttctta ttaatcgctc ccctaatcca cgcattcatc tgctctacag
      181 gctctaaaat attttttctg agtactgagc acaaactgct ccataaaaaa cagaccacaa
      241 gtttgggcat gatactgatg tagtcacgtt ttattgtttt catgaagctc tctgctattc
      301 cattactcac cggactccgc accgccgtgt tcttcgattt aagtcccaac atccgagcga
      361 actggcgtgt ttcattaacc cggtagtcag agtcagcggt cgaaagacct ccctggtagc
      421 tactctgcat acccactata atgcaactat tctcgtgacg tgtgtttcag ctctcccccg
      481 gctatgatac gagatggtat ggagagccct tctggggcca ctgcctgcac cagatgcaac
      541 aggaagcctg atatgaatgc aatttctgtc aggacaggcg ggtaactttc tgtggccatg
      601 gatcagggaa tactccgccc gacatccacc actgaatttc ttcttcagtc aacaagcaaa
      661 gggataaggc atgtgtaaaa tcattaactt cagtggcatt gccaatgata gtcaactgac
      721 agcagcgatc gccaaatcgc ccagaggctg tgtcgatgtg tcgctgtagc gctgaacgtt
      781 cttcacgagt taaattattg tcagtatgtt ccagtactcc agctttccag tagccaatga
      841 gcgccagact gatgcttcct gctgactgat tccagagcag ggcaagatca tctctcccgg
      901 ggagccaaaa aaaaccttta ctgcgataaa cgcccatgcc cataaagcgg tgacaggtat
      961 cccataatct ttgcggatgg aatggcctgt cgtccctgat gacgcgccat ataagttcct
     1021 cattgttgca cggagtatcc atgtttcctg caagtgcttc cagttcactg atgagtaact
     1081 caacacggtc aaaattgtag tccgggatcg ccagtaatgt gctcagttca atatttcccc
     1141 atgataccgc gagcacatcc acggaggggt taagtgggtg tatggctttt gccacagcgg
     1201 agataatgtc aaagggcagg tggtcatttc tggtcagcag cagcctgttg cagaacatga
     1261 tttgctcggc cagcaaattc tccaggccgc gtttgttatg ccggagattt tcctgaagag
     1321 tggggatcag ttttttccca tcattgtaat cttcatggag catgacagta tcaacgagtg
     1381 ttaaaaacgc cttcaggcgt acacgtgaat ggcgacgcag gtatttgacc agaggaagcg
     1441 gatgactgct tccggaggtt tcaatcagca tcacatccgg cttgttgtga tcaagcaggt
     1501 ggttaattgc gagatccatc tttttgaggc ctgcggggct gctgatgctg tctgcggtga
     1561 tacttacgaa attattgtct tttgcatcta caatatctgt gttggcgatc agcacgccgt
     1621 ccacatcaag ttcgctcatg tcattgacta tgacagagac tgaaagctgt ttttcgtgtg
     1681 cctgtatgag caggtttttc agcagagtgg ttttccctgc gccgaggaag ccattgagaa
     1741 tgatgacaga tgttgtttcc atatcagcag tacaccagtt agttagcgag gaaggcgctt
     1801 tgtggtacaa agagcatccg gattgaatcg cataacgtcg acgggagttg ttgtcgtttt
     1861 tcctgtgagg tctcaacctg ccacagaacg caccggattt attggtattt agtctccatc
     1921 agcataatta tcaccatttt tatctccggg gggaagattt gttctgaaca ggagtgg
//
LOCUS       DAFAVF010000304         1941 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFAVF010000304 DAFAVF010000000
VERSION     DAFAVF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.311"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..361)
                     /locus_tag="LGK42_004534"
     CDS             complement(<1..361)
                     /locus_tag="LGK42_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660681.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQ"
     gene            complement(369..>1941)
                     /locus_tag="LGK42_004535"
     CDS             complement(369..>1941)
                     /locus_tag="LGK42_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBK2660682.1"
                     /translation="SAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYF
                     MTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEI
                     ERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKI
                     VRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRI
                     HKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIR
                     HAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINEL
                     GYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKETGLKANS
                     LCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDS
                     QFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKP
                     MPFSAWDKSGKLVKE"
BASE COUNT          561 a          466 c          380 g          534 t
ORIGIN      
        1 cctgcataat atctgcacaa taatttttct cgaactcctt tacctgaata cattcgtcac
       61 tggaaatcag ataaggaccg acatcgtaca aataagaggg ggaaaccgag tcggcgtaag
      121 ctgtgcgcaa accaaccaac agctgttcct ccgtgggatt tagcgtgttg ataagatcaa
      181 ttgccacgct acgccgatat tcagcaatat ttgccaccgc aaaagcagta tcaataacgg
      241 actgtttcag gctatacacc cgttccttta ccatttgagt gtagatcccg caggcaactg
      301 ctgaaatcat gataaaaatc agcagactga ctgtaagtac gaacttattg attctggaca
      361 tacgtaaact actcttttac taattttcca cttttatccc aggcggagaa tggcatgggt
      421 ttctggtaca gatacccctg agcctgaaca catcctaatt taattaattc gcgcgcaacg
      481 tcttcgctct ctacgccttc cgcaatcacc gggatccggt tatagcgtgc cagatcgaaa
      541 ataatgcgca caatttttcc cgcagtaccg aaaatatcgc cggtaaattg cgagtccacc
      601 tttatgctgt ctggattaat ttcataaaaa cgtttcaggt ttgacaaacc ggtgccaaag
      661 tcatcaatcg atatccgtac cccttgttta cgcagttgtt caatattgag ataaaaatgt
      721 tcattaattc gctcaatcac ggtttcagtg atctcaacac agaggctgtt cgccttcagg
      781 ccagtttctt tcatgatggt ggtaaaacgc tcatgaaagt gcggttcgtt taactgccag
      841 ggcgaaacat taatatgcag taagaaatca tccttcagcg acgcacgctg actaaaatgt
      901 ctgaattcac ccattgccgt tttaataatc tgataaccca gctcattgat aaacccgata
      961 tcttcagcaa tggtaatgaa cttcagcggt gaaatgatcc cccgctccgc agaaacccaa
     1021 cggcacaacg cctccgcgcc cagaatggcg cgggtgttaa tatcaacgat cggttgatag
     1081 acgaggaata gttcgccaat ttccattgca tggcgaatat tacgcgcaat ccagatattc
     1141 tcttctattt cgttgagttt cgcgtcatca cagaaaataa actcggagaa catatggtca
     1201 cggatactgg aaatcgccag tttcagtttt tctgcgtaac tccaggcgtt ttccccctgc
     1261 aaaggttcac aaatggcaga cttatggatg cgcagattgc ggtagccaaa gctgctgtca
     1321 aaatcgttta ccatcgctat tttacgggta aatgtctgta cattttcccg acacagaagt
     1381 aaatagagat cggcattgaa tgtcacgata tcgcagcatt gcgcatattt ctcggcaatt
     1441 cgctgacaca atttcctgac gattttattg cgttcatcaa caccaaacac cgcttccacg
     1501 gcttccagac cacacagttt gatgatcccc acagccagat actgattatt gttggactca
     1561 ataatcgcca tatttttaat aaaaccgctt tcctgatcgt aagagatcag tgagagcaat
     1621 ttatttgacc gttcaatttc ggcatctttc atttccgcga tcgaattttt gatgctgata
     1681 atctctttcg catcgccttc gcgaattttc agagcctcga tctgatccgt tgagtcgggc
     1741 aaaaacgcca gcgtattaac cagctcggtc atttcagaaa attgccgacg gaacacacga
     1801 gtggtcatga aatataccac cagcaacacg ataataatga acgcaaacgg tagataaaaa
     1861 agtttatctg atgcgttcaa taaattaccg ccaggataat aaaaatagat ttcgaacaat
     1921 gcattatttg gcactgctga t
//
LOCUS       DAFAVF010000305         1918 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFAVF010000305 DAFAVF010000000
VERSION     DAFAVF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.312"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..206)
                     /locus_tag="LGK42_004536"
     CDS             complement(<1..206)
                     /locus_tag="LGK42_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HBK2660683.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGAL"
     gene            complement(261..1112)
                     /gene="manZ"
                     /locus_tag="LGK42_004537"
     CDS             complement(261..1112)
                     /gene="manZ"
                     /locus_tag="LGK42_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBK2660684.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1125..1901)
                     /gene="manY"
                     /locus_tag="LGK42_004538"
     CDS             complement(1125..1901)
                     /gene="manY"
                     /locus_tag="LGK42_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004721310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBK2660685.1"
                     /translation="MLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLGDMKTGIII
                     GGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPLAAAGQVLT
                     IIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVALSVGTSEVQ
                     NMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAAFTNFNLVA
                     LGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
BASE COUNT          528 a          500 c          500 g          390 t
ORIGIN      
        1 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
       61 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      121 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      181 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      241 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      301 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag tagccacata
      361 caagcaaagg tcagcagcag tggtaccagg cctggcatta actggtccag aatagtctgg
      421 acagtagtaa cgtgttcttt gcccgtctgg tcagtaatgc gagagacaac cagcgggatg
      481 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      541 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      601 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      661 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      721 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
      781 aaaccgactt tgataccgtt gatagcaccg tcgtcgatct ctgcgccatt agcacgctgt
      841 tcttccagcg ccagggttac gccgagaatc ggcgcagcca cgaacggctg ggtgttaaag
      901 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
      961 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1021 gaaccctgga agaggttaga acgcaggaag acgccacgaa tatcactttg agtgagtttt
     1081 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1141 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1201 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1261 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1321 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccacc ggcgatattc
     1381 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1441 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1501 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1561 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1621 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1681 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1741 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     1801 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     1861 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaa
//
LOCUS       DAFAVF010000306         1908 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFAVF010000306 DAFAVF010000000
VERSION     DAFAVF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.313"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(130..522)
                     /locus_tag="LGK42_004539"
     CDS             complement(130..522)
                     /locus_tag="LGK42_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HBK2660686.1"
                     /translation="MKISYLALILTFIACVAGGLVWSANHYYGKFLEEQKRADAAEQR
                     ADSTEAITENVLRTMAITNIIQEANQHAKQQIALESQRTQEDIKVAIANDDCASRPVP
                     ATAADRLRKYADSLREYTGGTTANQPDF"
     gene            complement(519..1133)
                     /locus_tag="LGK42_004540"
     CDS             complement(519..1133)
                     /locus_tag="LGK42_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HBK2660687.1"
                     /translation="MNQQLFQKAAGISAELAARWFPHIDAAMKEFGITAPADQAMFIA
                     QIGHESMGFSAVVENFNYTPSALVATFGKRITQQQADALGRTSGHAARQDAIANLVYS
                     NRLGNKAPGDGWKYRGRGLIQITGLHNYRICGAALKLDLVTSPEQLEQELQAARSAAW
                     FYTSKGCMIYGADINRVTRIINGGLNGIEDRKVRYNRARAVLLV"
     gene            complement(1133..1414)
                     /locus_tag="LGK42_004541"
     CDS             complement(1133..1414)
                     /locus_tag="LGK42_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2660688.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            complement(1401..1787)
                     /locus_tag="LGK42_004542"
     CDS             complement(1401..1787)
                     /locus_tag="LGK42_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK2660689.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLANLTGYSDKPLDAIGAVIVSALAVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
BASE COUNT          513 a          513 c          424 g          458 t
ORIGIN      
        1 cgtttttctt catgccttct aatatgagac ttatctaaat tacaactccc taacgcggac
       61 aacagtttta cattcaaatc caaactttca ccatacgtca gcggattggg tataaaaggt
      121 actggagtat cagaagtcag gctggttggc agtagtaccg ccggtgtatt cacgtaaact
      181 gtccgcgtac ttccgcaacc ggtcagcagc ggtagcaggc acaggacgtg aagcgcaatc
      241 atcattcgca atagccactt tgatatcttc ctgggttctc tgtgactcca gtgcgatctg
      301 ctgttttgca tgttgattcg cctcctgaat gatgttcgtt attgccatag tacgcagaac
      361 attctcggtg atagcctcag tagaatcagc tcgctgttcc gcagcgtcag cacgcttctg
      421 ctcctccaga aactttccat aatagtgatt cgctgaccag acaagaccac cagcaacaca
      481 agcaataaac gttaaaatga gcgccaaata acttatcttc ataccagcag caccgcccgc
      541 gccctgttgt atcggacctt acgatcctca ataccgttca gaccgccgtt aataatgcgc
      601 gtaacacggt taatatcggc accgtagatc atgcagcctt tagaggtgta gaaccatgca
      661 gctgagcgcg ccgcctgtag ttcctgttcc agttgttcag gtgaagtcac cagatctaac
      721 ttcagcgccg cgccacagat gcgataatta tggaggcctg tgatttgaat taatcctcta
      781 ccacgatatt tccagccatc acctggtgct ttgttaccca gtcggttgct atacaccaga
      841 ttggcaatag catcctgacg agctgcatgt ccggatgttc tgccaagggc atcagcctgc
      901 tgctgtgtga tcctctttcc gaacgtcgcc accagcgcag atggtgtgta gttaaaattt
      961 tcaactacgg cgctaaaccc catcgactca tggcctatct gagcgataaa cattgcctga
     1021 tccgctggtg ctgtaatgcc gaattccttc atcgccgcat caatatgcgg aaaccagcgc
     1081 gcagccagtt cggcgctaat accagccgcc ttttgaaata attgttggtt cattagtgcc
     1141 tcagatgatc aaccagacgt gcaacgttgc ctctgacggc caccagcacg gaaagaaaaa
     1201 tagtgttcgc cacgataatg ggccatgagg aatggggata aatcccacag agataggcca
     1261 acggaacagc actgtatgta acagtaatca gccaggctaa acgtgaaacc caaggacgat
     1321 gccgcgaatc accacgacga taaaacatca gagtaataac aacacaagca cataacagcg
     1381 catttatagt tgctgtcggg tcatttagct ccacctgaac ctccccggcg cgttatgagc
     1441 gccaccagcg agccgatatc ctgattattc aggaacgtca ggattttaac ggctaaagca
     1501 gagacgatta cggcaccaat agcatccaga ggtttatcac tgtatccggt caagttcgcc
     1561 agcttggagc caaccaaccc agagcaaaga atcccggcaa tatatgacac gataaaatat
     1621 gccagtcggc gcgatgcact cagatctgct gctgttgcta tgtagaatac agcccctgca
     1681 aatgcgccaa atacaacgcc gtaatcagtt ccggtcagca gtccataaac actggcaccc
     1741 gtcagggcac caccagccag cccagtaccg gaaatcggat cggacattta gccccctctt
     1801 aattgctgtt ggtcctctca gatatgaggg gaagggatct taatgacagt ctgtttatta
     1861 tttcagtcaa acactaccct gttgatgatt tctcagaagc gaacttga
//
LOCUS       DAFAVF010000307         1905 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFAVF010000307 DAFAVF010000000
VERSION     DAFAVF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.314"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(245..320)
                     /locus_tag="LGK42_004543"
     tRNA            complement(245..320)
                     /locus_tag="LGK42_004543"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Asn,seq:gtt)
     gene            complement(421..1893)
                     /gene="yeeO"
                     /locus_tag="LGK42_004544"
     CDS             complement(421..1893)
                     /gene="yeeO"
                     /locus_tag="LGK42_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014832583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBK2660690.1"
                     /translation="MQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPI
                     FMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSL
                     GKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLE
                     LTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGF
                     VGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASV
                     ESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGV
                     GQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNAL
                     FMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGM
                     FADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          583 a          518 c          390 g          414 t
ORIGIN      
        1 atcccgcata aggcattttt ctctttatct ttgtacggta cttcatggaa cagaattttt
       61 gaccttgtga atcgtgatgt ctgttgggga ggagcaattt gctcactgaa gcgtgagact
      121 cgattaagcg cacgaaacac agaaatcaaa aaacccggcc acttttttac aaactgaccg
      181 ggtaaaaata atttttattt tttaactgcc ttgagactca tagagatgtc tcaaaactaa
      241 aatttggctc ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct
      301 accgactgaa ctacagagga atcgtgagaa cgaggcgaat attagcgatg cccacccaca
      361 atgtcaaagc ctgtttttta aatttgaaat cgtttgctga aataatctgc attttgttgt
      421 ttattccgac acaactggct ttttttcaca cttttgcggt tcgggtcgag ggtatttcca
      481 tagccaacgt ccagtaacca ttcgccagta aaacagcacg gcccgcacag cccagtcggc
      541 aaacattccc atccagacac caaccacacc ccagccaagc atgatgccca gcacataacc
      601 gacgacaacc cggcaacccc acatgctcaa catagaaacc cacatagcgt aacgggcgtc
      661 acgagccccc ttaaatccag cgggtagcac ccatgaggcg gaccaaatag gcataaataa
      721 agcatttagc caaatcagaa tcacaacgac atgtttaacc tgtggatcct gggtgtaaaa
      781 cgatgccata accccggcaa agggagccgt tagccaggcg atggccgtta atccaagagt
      841 ggacagccag aagacatgcc gcaactgaat ctctgcttgc gctatctgcc ctacccctaa
      901 ccttcggcct gtaatgatcg tagaagcaga gccgagcgca cttccgggta agttgataag
      961 agccgcaatt gaaaacgcga taaaatttcc ggcaataaca ctggtcccca tcccggcaac
     1021 gaacatttgg gttaataacc gaccactggt aaataacact gattcgacac tcgctggaat
     1081 accaatcccc atgacttccc agataatgct aaaattcagc ggtttaaaat agctctttaa
     1141 cgaaatcctt agcgcaggat taaaaccaat cgccagcacc cacaaaattg caactgcgcc
     1201 aatataacga gaaatggtta aacccaaccc agccccgaca aatcccagtc ccggccagga
     1261 gaaaaggccg taaatcaata tgccgctaat aataatatta agaatattca ggctaccgtt
     1321 aatcagtagc ggtattttcg tattccctgc accacgaagt gccccgctac caatcaaagt
     1381 gatggcagct gctggataac tgagtaccgt cagctccaga taagtcaacg ccagtgcttt
     1441 aacttctgtc gtggcatcac ccgcgacgaa atcaataatt tgctcgccaa aatgatgaat
     1501 aagcgttgcc aacagtacgg caaacaacgt catgatcacc aatgactgcc gcgtcgccac
     1561 cctcgctcgt cgtcgatccc gcttaccgag actaaatgcc acaacgacag tagtaccaag
     1621 atcgatagca gcaaaaaaag ccataatgac catattgaag ctgtccgcca atcccacgcc
     1681 ggccatcgca tcttttccca gccagctgac cagaaaagtg ctcagaaccc ccatcaacag
     1741 gacacaggca ttctccatga agataggaac agcaagcggg gttatctcgc gccagaacaa
     1801 cactttgtag ctcttgcgtt tagcgtgcca gcgagtgccg tgaacaacct ggcgtaaagc
     1861 agaggagata ttcaaagccg accttaattg cagaaagtga aacca
//
LOCUS       DAFAVF010000308         1904 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.315, whole genome shotgun sequence.
ACCESSION   DAFAVF010000308 DAFAVF010000000
VERSION     DAFAVF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.315"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(189..1595)
                     /gene="gndA"
                     /locus_tag="LGK42_004545"
     CDS             complement(189..1595)
                     /gene="gndA"
                     /locus_tag="LGK42_004545"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK2660691.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPHYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDDNYLVDMILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1692..>1904)
                     /locus_tag="LGK42_004546"
     CDS             complement(1692..>1904)
                     /locus_tag="LGK42_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBK2660692.1"
                     /translation="SYLSKKMFIVMIVSLFFNLILIRFYGIHGAAYATLITELLSFTL
                     LNYFYKNGIVFKMHLKILTAYRKNSV"
BASE COUNT          524 a          438 c          420 g          522 t
ORIGIN      
        1 atctactgcg ggatttttaa cgctgatgta gatgaactgt caaggcagcg atcctgctgt
       61 gcggctctgt attatatcgc ttttttaacc ataaattata aaaaaaggcc ataacctgcc
      121 gctttgtata ataaaaaagc ccggagggtt tctccgggcc ttgcattgat taattgattt
      181 aaatcagatt aatccagcca ttcggtatgg aacacacctt ctttatcaat gcgcttataa
      241 gtatgcgcac cgaaatagtc acgctgtgcc tggatcaggt tcgcaggcag aacagcggca
      301 cggtagctgt cataataggc aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt
      361 actgcataag caacgacatc acgcagcgcc tgctggtaat catcggcaat ttgtttaaag
      421 tacggagcca gcagcaggtt agcgatctgc ggattttcgg catatgcatc ggtgattttc
      481 tgcaggaact gcgcacggat gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag
      541 ttcagatccc agttgtactc ttcagacgcg gcgcgtagct gagagaagcc ctgggcgtaa
      601 gaaacgattt tgcccagata cagagcacga cgaacttttt cgatgaactc agccttgtcg
      661 ccagctggct gcgcttgcgg gccagagaga actttagacg cggcaacgcg ctgatctttc
      721 agagacgaga tataacgtgc aaacacagac tcggtaataa gcgacagtgg ttcaccaaga
      781 tccagcgcgc tctggctggt ccatttaccg gtacctttgt ttgctgcttc atccagaatc
      841 atatcaacca ggtagttatc gtcttcatct tttttggtga agatgtcttt ggtgatgtcg
      901 atcaggtagc tgctcagttc accgttattc cactcggtaa aggtctgcgc cagttcttcg
      961 ttggtgaggt tcaggccacc tttaagcagt gaataggctt cagcaatcag ctgcatatcg
     1021 ccgtattcaa taccgttgtg aaccatcttc acatagtgac ctgcaccatc ggcaccaata
     1081 taggtaacgc atggctcacc gtcttcagcc actgcggcga ttttagtcag gatcggtgca
     1141 acaagttcat aggcttcttt ctggccgcca ggcataatgg aaggaccttt cagcgcgcct
     1201 tcttcaccac cagaaacacc ggtaccaatg aagttaaagc cttctgcaga aagctcacga
     1261 ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat gtcaccttta
     1321 tcgaggtatg gcttgagaga atcaatagca gcatccgtgc ctgcacctgc tttcaccatt
     1381 aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt atagtgagga
     1441 accagtttct tgcctggatt ttcggcaatg acttcttccg tcttttcacg ggaacggttg
     1501 aaaatagaga cggtataacc gcggctttcg atgttgagcg caaggttgcg ccccatcact
     1561 gccataccga cgacgccgat ctgttgcttt gacattgttt actcctgtca gggattctac
     1621 ttatttgatt attaaggata gccaagctat caatattatt aatcatatta taacatactc
     1681 aaaaaaaaag tttacacact attctttcta tatgctgtta aaattttaag atgcatttta
     1741 aaaacaattc catttttata aaaataattc aataatgtaa atgataataa ttcagtaatg
     1801 agagtagcat atgctgcgcc gtgaatccca taaaacctta tcagaattag attaaaaaac
     1861 aaactaacta tcattactat aaacattttt ttggataagt aaga
//
LOCUS       DAFAVF010000309         1903 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFAVF010000309 DAFAVF010000000
VERSION     DAFAVF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.316"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1620
                     /locus_tag="LGK42_004547"
     CDS             <1..1620
                     /locus_tag="LGK42_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBK2660693.1"
                     /translation="NGSQVNVSESKLTSDTIDLTNGSSLNIGEDGYVDTDHLTINSYS
                     TVALTESTGWGADYNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNG
                     NVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGT
                     ELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADL
                     GAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLA
                     DNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDM
                     NDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSD
                     AWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGV
                     DAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFT
                     KNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1708..>1903
                     /locus_tag="LGK42_004548"
     CDS             1708..>1903
                     /locus_tag="LGK42_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK2660694.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGIT"
BASE COUNT          504 a          439 c          458 g          502 t
ORIGIN      
        1 aatggttctc aggttaatgt ttcggaatct aaattaactt cagatactat cgatttaact
       61 aacggttctt cgctgaacat tggtgaagat ggctacgttg ataccgatca tctgactatt
      121 aactcctaca gtactgttgc gttgaccgaa tctactgggt ggggggctga ttacaacctg
      181 tacgccaata ctatcaccgt aactaacggt ggtgtattgg atgtgaacgt tgatcagttc
      241 gatactgaag ctttccgtac tgacaaactg gaactgacca gcggcaacat cgctgaccat
      301 aacggtaacg tagtatctgg tgtgttcgat atccatagca gcgattacgt tctgaacgct
      361 gatctggtga acgaccgtac gtgggatact tccaagtcta actacggtta cggtattgtt
      421 gctatgaact ctgacggtca cctgactatc aatggtaacg gcgacgtaga caacggtact
      481 gaactggata acagctctgt tgataacgtt gttgctgcaa ccggtaacta caaagttcgt
      541 atcgacaacg caactggcgc tggcgctatc gctgattaca aagataaaga aattatctac
      601 gtaaacgacg tcaacaccaa cgcgaccttc tctgctgcta acaaagctga cctgggtgca
      661 tacacctatc aggctgaaca gcgcggtaac accgttgttc tgcaacagat ggagctgacc
      721 gactacgcta acatggcact gagcatcccg tctgcgaaca ccaatatctg gaacctggaa
      781 caagacaccg ttggtactcg tctgaccaac tctcgtcatg gcctggctga taacggcggc
      841 gcatgggtaa gctacttcgg tggtaacttc aacggcgaca acggcaccat caactatgat
      901 caggatgtta acggcatcat ggtcggtgtt gataccaaaa ttgacggtaa caacgctaag
      961 tggatcgtcg gtgcggctgc aggcttcgct aaaggtgaca tgaatgaccg ttctggtcag
     1021 gtggatcaag acagccagac tgcctacatc tactcttctg ctcacttcgc gaacaacgtc
     1081 tttgttgatg gtagcttgag ctactctcac ttcaacaacg acctgtctgc aaccatgagc
     1141 aacggtactt acgttgacgg tagcaccaac tctgacgcgt ggggcttcgg tttgaaagcc
     1201 ggttacgact tcaaactggg tgatgctggt tacgtgactc cttacggcag cgtttctggt
     1261 ctgttccagt ctggtgatga ctaccagctg agcaacgaca tgaaagttga cggtcagtct
     1321 tacgacagca tgcgttatga actgggtgta gatgcaggtt ataccttcac ctacagcgaa
     1381 gatcaggctc tgactccgta cttcaaactg gcttacgtct acgacgactc taacaacgat
     1441 aacgatgtga acggtgattc catcgataac ggtactgaag ggtctgcggt acgtgttggt
     1501 ctgggtactc agttcagctt caccaagaac ttcagcgcct ataccgatgc taactacctc
     1561 ggtggtggtg acgtagatca agactggtcc gcgaacgtgg gtgttaaata tacctggtaa
     1621 tattcttcac tccgaagaaa ttctggtaat ttaatctaaa taatgcccgt caaggatttg
     1681 acgggcatta ctgcaaagga cgcgcaaatg ttatctgtag ttaaacctct tcaggaattt
     1741 ggtaagctcg ataaatgttt gtccagatac ggtacgcgct tcgagtttaa taatgaaaag
     1801 caagttatat tttccagtga tgtcaatagc gaagatactt tcgttatttt agagggcgtt
     1861 atctctctgc gtagagaaga aaacgtactt atcggtatta ccc
//
LOCUS       DAFAVF010000310         1860 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFAVF010000310 DAFAVF010000000
VERSION     DAFAVF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.317"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            94..1098
                     /gene="msrP"
                     /locus_tag="LGK42_004549"
     CDS             94..1098
                     /gene="msrP"
                     /locus_tag="LGK42_004549"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBK2660695.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            1099..1734
                     /gene="msrQ"
                     /locus_tag="LGK42_004550"
     CDS             1099..1734
                     /gene="msrQ"
                     /locus_tag="LGK42_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBK2660696.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
BASE COUNT          447 a          461 c          469 g          483 t
ORIGIN      
        1 aagcagatct ctgctactgt tagggaggac acttcctgac agattaacag taaacggctc
       61 ttgctggcta aagacaaaaa agtgtgatgg cttatgaaaa agaatcaatt tttaaaagaa
      121 tcagatgtta cggccgagtc ggtattcttt atgacgcgtc ggcaggtgtt aaaagcactg
      181 ggcatcagcg cggctgcact ttctttgcct cacgctgcgc atgccgatct gcttagctgg
      241 tttaaaggga atgatcgccc acccgccccc gccggaaaac cgttggagtt cagcaagcct
      301 gccgcctggc aaaatgacct gccactgacg ccagcagata aagtctccgg ttataacaac
      361 ttctatgaat tcgggctgga taaagccgat cccgccgcta atgctggtag cctgaaaacc
      421 gatccatgga cactgaaaat cagcggcgaa gtggcaaaac cattgaccct cgatcacgat
      481 gatttaaccc gtcgctttcc gctggaagag cgtatttatc gtatgcgctg cgtggaagca
      541 tggtcgatgg tggtgccgtg gattggtttt ccgctgcaca aattgctggc gcttgccgaa
      601 cccaccagca atgcgaagta tgtcgctttc gaaacaattt atgcaccgga acaaatgcca
      661 ggccagcagg accgctttat cggtggcggg ctgaaatatc cttatgtcga aggattgcgt
      721 ctcgacgaag caatgcatcc gctcacactg atgaccgtgg gtgtttatgg caaggcgtta
      781 ccgccacaaa atggcgcgcc ggtgcgactg attgtgccgt ggaaatatgg ctttaaaggg
      841 attaaatcga tagtcagtat taagctgact cgcgagcgtc cgccaaccac ctggaatctg
      901 gcagcgcctg acgaatacgg tttttacgcc aacgttaatc cgcatgttga tcacccgcgc
      961 tggtcacagg ctaccgaacg atttattggt tcaggcggca tcctcgatgt acagcgccag
     1021 ccaacgctac tgtttaatgg ttacgccgag caggtggcat cgctgtatcg tggcctggat
     1081 ttgcgggaga atttctaaat gcgtctgaca gcaaaacagg tgacatggct gaaagtttgc
     1141 ctgcatcttg ccggattgtt gccgtttctc tggctggtct gggcgattaa tcacggtgga
     1201 ttgggtgccg atccggtgaa agatattcag cattttactg gtcgcactgc actgaaattt
     1261 ttgctggcga ccttgttaat cacacctctg gcacgctacg caaaacagcc gttattgata
     1321 cgcactcgcc gcctgttagg cttatggtgc tttgcctggg cgacactgca tttaaccagt
     1381 tacgcattgc tggagctggg cgtgaataat ctggcgttat taggtaagga gttaattacc
     1441 cggccttatt taacgttagg tattatcagc tgggtaattt tgcttgcgtt agcgttcact
     1501 tcaacccagg cgatgcagcg aaaactcggt aaacactggc aacagttgca taacttcgtc
     1561 tatcttgtcg cgatcctggc cccaatacat tatttgtggt cagtgaagat tatttcaccg
     1621 cagccactca tctacgctgg gctggcggta ctgcttttag ccttacggta taagaagttg
     1681 ctttcccttt ttaaccggtt acgcaagcag gttcacaaca aattatctgt ataacttcat
     1741 attgccgaca aagtacgcgt tatccctgcc ggatgcggcg cgagcgccct atccggccta
     1801 cgcagatcac atcaagttat gtttcgtttg tcatcaacct caaatcatcg acataattta
//
LOCUS       DAFAVF010000311         1841 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFAVF010000311 DAFAVF010000000
VERSION     DAFAVF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.318"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..143)
                     /locus_tag="LGK42_004551"
     CDS             complement(<1..143)
                     /locus_tag="LGK42_004551"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK2660697.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHI"
     gene            complement(439..1125)
                     /locus_tag="LGK42_004552"
                     /pseudo
     CDS             complement(439..1125)
                     /locus_tag="LGK42_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1176..1465)
                     /locus_tag="LGK42_004553"
                     /pseudo
     CDS             complement(1176..1465)
                     /locus_tag="LGK42_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          473 a          478 c          421 g          469 t
ORIGIN      
        1 ttgatatggc gttcactgtt atattctgac aaccagtttg ccgccagttc ccgcttttca
       61 tccagcgttc tgaacagaca aaaaccgagt atttttgttc ggtatgtccg gttaaaactc
      121 tcgataaaag cgttctgcgc caacttacct gattgggtaa tctccagttt tactacatgt
      181 ttttctgcgc attacgtccg tgttggataa aaaaaatcct ggccataatc catgcgaatc
      241 atagctggat agacatggtt ttccgcaatc ctacagagta ccgctaccac ttgtgaagct
      301 ggcagattca gattgttttc aatcgacaac gcctcatagt taaagtcatc aacgacattg
      361 aacgtgtgaa gtggtcaaca aaaactggcc accgagttag agtttttcca gtatcgattt
      421 tccggtaatg actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt
      481 gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg
      541 ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg
      601 cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac
      661 gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc gttcaccgaa
      721 tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc agcgctggcg
      781 cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta
      841 tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc
      901 aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat
      961 tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg
     1021 gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc
     1081 gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa
     1141 acaccatcat acactaaatc agtaagttgg cagcatcacc gattttccga ttcgtttggg
     1201 gataacccac cgttatattc gtgcggtctt agtgcgctgt aatatccaac gatatagacc
     1261 gttatggcgt gagcggcctc gcagaagctt cgtaacccac caccggcatc cattcgttct
     1321 tcagactcct gaagaagcgt tccattgggc tgttatccca gcagtttccg cgccggctca
     1381 tactctgcct gatctggtat cgccacaata actgccggaa ctgcctgctc gtatcgctgt
     1441 ggaacatcac cccgccgggc ttacccgtcc agatataggt cacatcaccg caccacacct
     1501 gtatagcggc gtaatcccca aaaacgatgc tggttgtcag taaacaagcc atgaatgaca
     1561 gtcgttcctg aaatcataag acctggagga gtgtatgctc cgtcgcagta attaccacga
     1621 tacgcttgca gaattttttc cagttactga agcgtgaacg gataaagaaa aagctcttcg
     1681 gaacgcggga agacgcctcc aacgatattt ttgaacatcg aaatgtttta taacaataag
     1741 cgtcggcatg gttcctgcga acagatgtca acaatagaat ataaaaacaa ttattatcaa
     1801 caacttggaa ggagtaagac tacccgtggc gattcaatat t
//
LOCUS       DAFAVF010000312         1838 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.319, whole genome shotgun sequence.
ACCESSION   DAFAVF010000312 DAFAVF010000000
VERSION     DAFAVF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.319"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(139..603)
                     /gene="pdxI"
                     /locus_tag="LGK42_004554"
                     /pseudo
     CDS             complement(139..603)
                     /gene="pdxI"
                     /locus_tag="LGK42_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
     gene            complement(610..1787)
                     /locus_tag="LGK42_004555"
     CDS             complement(join(610..1529,1529..1787))
                     /locus_tag="LGK42_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK2660698.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQRINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT
                     "
BASE COUNT          410 a          496 c          459 g          473 t
ORIGIN      
        1 ttccgcctgt actggaagac tcagcataaa caggggaagt aaacctatcc agctgcaaat
       61 cctgggctta aacatatgca ctccatgcca ttaaaaaaga aacaaaaggg aaatgcatct
      121 ttcccttttg tatattcgtt attctcgcga aataccatcc aacgtagaca acacttcctc
      181 agaaagatgc aatttttcag cagccatatt ctcccgtaaa tgcgcaacag aagacgtccc
      241 tgggatcagc aaaatattcg gtgaacgttg taacagccac gccagcgcca cctgcatggg
      301 tgttgcaccc aggctcgcag caacatccga cagcgtagag gattgcagcg gtgtaaagcc
      361 cccgagcggg aagaacggca cgtaggcaat gccatcgtgg gccaaagcat caatcattgc
      421 atcatcagca cggtgcgcga tgttgtattc gttttgcaca cagacaattt cggcaatctt
      481 gcgcgcctct gcaacctgcg tcggtgtgac gttgctcagg ccaatatgtt ttaccaggcc
      541 ttgttgttgc atctctgcca gcacggtcag gctggcctca atcgatcctt ccgctgggcc
      601 atgtagtggt caagtaatat tggccacggt tttacagtaa aaatggtatc tgttctctga
      661 ctcttccggc gtcagcccac cgttataatg gtgaggtctg acgctgttgt agtagttcaa
      721 tatgtaatca ttaattcgct gccgggcctc gtccttgcct gcgtaaccat tcgttggcac
      781 ccattctgtt ttcagactgc ggaagaagcg ttccatgggg ctgttatccc agcagtttcc
      841 ccgccgactg acgctttgat ttattctgca acgccagaga agttgttgat atttaaggcc
      901 tgtatactgg cttccctggt cgctatggaa cacgacatcc cggggttggc cacgcgtctc
      961 ataggccatc cgcagggcac tgcttatcag tgcggtatcg gcatgcgctg acagactcca
     1021 gccgataacc ctgcgggcaa aaagatccat aacaaccgcc agatagcacc agcgatttcc
     1081 tgcccagaga tacgtaatat ctccacacca tacccggtct ggctccggta ctgcgaactg
     1141 acgctcaagc agattcggca ggctggtatg ctcctgacgg gcatttttat actgatgttt
     1201 tccgggctga caactgctca ggttcagata tttcatcaga cgtccggcac ggtaacggct
     1261 catcgggacg ccgttttgag tcagcatttc agccagagtg cgcgcgcccg cagagccccg
     1321 actttggttc cacgcccggc gtatttcgct gcacaacctg actcgtgccg gattaaccgt
     1381 atcgcgtcgt tttcgccagt accggtaact gctgcggtgt atttccagag cagaacacag
     1441 gctgacaacc gtgtggctgt cacttagtct ggcggctatc gtgaaccgtt cagcgagtcg
     1501 gacattaaga gcgcggtagc cttttttaat atcgtatttt gttcctccag acggcgaact
     1561 tgcttttcca gctcgcggat acgttgctgg tctggagtaa tgggtgtggc agagggcgta
     1621 ataccctggc gctctcgcct gagctggcgt acccagctct caagcgtggt tgaaccgaca
     1681 ttcatcgctt cactggcctg tcgatatgag tagcccttat caacaatcag ctgtgcacat
     1741 tccagcctga actcaggggt gaaggttcgt ttggttttct tgttcattaa gtcacctgtt
     1801 ttgtgttgtg gtgagaatat cacctttcat caggtggc
//
LOCUS       DAFAVF010000313         1826 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFAVF010000313 DAFAVF010000000
VERSION     DAFAVF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.321"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..216
                     /locus_tag="LGK42_004556"
     CDS             <1..216
                     /locus_tag="LGK42_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK2660699.1"
                     /translation="KEINGSVIYPALSLSPTLLNDEKRKWLTEYTTRQNTTMSLRRVT
                     LRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            301..1503
                     /locus_tag="LGK42_004557"
     CDS             301..1503
                     /locus_tag="LGK42_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBK2660700.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            1563..>1826
                     /locus_tag="LGK42_004558"
     CDS             1563..>1826
                     /locus_tag="LGK42_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK2660701.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKF"
BASE COUNT          529 a          419 c          395 g          483 t
ORIGIN      
        1 aaagaaataa atggctctgt aatatatccg gcactatcat tatcaccaac acttctgaac
       61 gatgaaaaaa gaaaatggtt aactgaatat acaaccaggc agaatacaac aatgtctttg
      121 cgacgcgtca cgttgagacc tggccttgcc gggaacctta ttacagggca aatactacct
      181 gttagcttat ttaatacgtc tttattaccc tggtaaatac cagatagtac caaagtaaaa
      241 attacggcac atccgtgtca tagcgttgtt ttgtctaaat tctgataaac aggaaattaa
      301 atgatgaaga aaaatcatat tacccgcacc atcattgcat ctgctgtatt attttccttt
      361 aacgctgcag cagcgacgtc atattttgaa gcgcgtaacg atgcaatggg tggtaccggg
      421 gtagcatctt cccattatgg tgtggcacct ctggcaaacc cggctttact gacaaagcac
      481 aacagcaatg atgatttcag ccttcttctg ccgtctgttg gggcacaagt tgctgatcct
      541 gatgatgtat caaataaagc tgatgatgtt aaagatgact gggatttgtt tgacagtgct
      601 gtagataacc agcatggcgt acaacaggca gcggcaaatc ttaaacatcg tctgcaggaa
      661 ttccgcaata tcaatgcaga tgctcaggta ggtgtatcag ctgtagcagc catggcaaat
      721 gacactctgc cgtttgccct tatggtcaag tcttacggaa ctgtcagcgt aaacggaaaa
      781 gtaaacgatg cagacctgga ttatctggac aaagttgcca atggaacaat taccgacgtc
      841 gataagaatg ccctgacctc acgcgcattc gggcgtgcag ctgtaattac cgacgtcgga
      901 atttcatttg ccaaagaact ggaaacggcc ggacaaaaat ggtctcttgg cgtcacacca
      961 aaatatcagc gtgtggacct gtttaactac aacgtaacgg ttcgtgatta cgacaaagac
     1021 gattttgacg gtgacaagta ccacaacacg aaaaacggat tcaacgccga tattggtgct
     1081 tatacagacc tgaatgacaa ctggactgtc ggtcttgttg cccagaacat cattccacgc
     1141 agtattgata ctaaagtcgt caatggcttt aaagaaacct tcaaagttcg tccgcaggca
     1201 accgccggtg tatcctggca taatgacctg tttaccactg ctcttgacat tgatctgact
     1261 cctgccagtg gcttcacttc tgacagcaaa cgtcagtttg caagtgttgg tgctgaattt
     1321 aacgcatgga aatgggcgca attgcgtgcc ggttatcgtc agaacatggc atcaaactcc
     1381 ggaagcgcct tcaccgctgg cgttggtatc tctccatttg acgtcgtaca tatcgatgtt
     1441 tccggcctgg taggaactga ccacgattac ggtgcaatgg cacaacttca gttcactttc
     1501 tgattactgt ccccgccccc ggcactaaac ccgggggtat ttttatattc agtttaaata
     1561 acatgatgaa accacgttct tcttattcaa aaacagcatt cattctttta ttttctgttt
     1621 tcctggtagc ggcagtgaca aaagcaaaaa gctcgcttcc cgatatcacc ctggaacagg
     1681 caaaagaaat taatgctgat aacaccgtta ttttcctttt ccgccatgga gaacgttgcg
     1741 accgttcaga tatgccatgc tattcggata aaagcggaat cactattacc ggtacagaga
     1801 aagcacagca ggaaggtatt aagttc
//
LOCUS       DAFAVF010000314         1813 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFAVF010000314 DAFAVF010000000
VERSION     DAFAVF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.322"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..347)
                     /locus_tag="LGK42_004559"
     CDS             complement(<1..347)
                     /locus_tag="LGK42_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660702.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSL"
     gene            complement(434..640)
                     /locus_tag="LGK42_004560"
     CDS             complement(434..640)
                     /locus_tag="LGK42_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBK2660703.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1399..1548
                     /locus_tag="LGK42_004561"
     CDS             1399..1548
                     /locus_tag="LGK42_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBK2660704.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          472 a          387 c          519 g          435 t
ORIGIN      
        1 agcgaacgct gcctcagacg ttcacattca gacaggagaa tgccttccag catcggatga
       61 tgaaccagca gatgttcact ggtgcataaa ttcactatca gatcatcatc caggggcggc
      121 gtggtccgtt tgtccggacc ataaatcagc cggtgcagac tctggggtac gatggcaatc
      181 tccggttttt caccattgaa tgacacccac ccgggcgtca gattatggaa gtagtagata
      241 ttcggatgac acacgcccca ggatacggcg tgccgggtga agccggtcag ccatttctgg
      301 tagctttcgt ggagcagctc gtaacgggtc tgatactgtg gtttcatttt catattcctc
      361 acgggggata acaggcagca gaacgccgcc ggcaggccgg agactgcgtc tgttgcagat
      421 gaacggttaa gaattacgga cgggactgtg caatacgcgc ctgcagccag gcttccactt
      481 cgcttttcag ccatcgggag ctgcggccca ttttgatggg cgcaggaaag tcaccatcct
      541 tgatgagttt gtaaaaccac ttatcggtca ggccggtcag ttgagtgata aatgccatgt
      601 cgaccatctg gtcatccatc agtgaaactg gggtgagcat gagaatgttc ctccggatgt
      661 tgttaaacca gccggtgtgg tcatgaaaac aacgcaccac cttcaccggc ataacaaccg
      721 gggagaaacg taaaaaataa tcgggaatgt gaccgccagg agataaacgg ggaggttacg
      781 gtggctgagc cgtaccggta tgccgggtgg ttgttacgga acgatgcttc cgcacgcgcg
      841 cgcgataaag aagggatgat tttcgtgagg aagaggggaa aactggcgtt ctgataattg
      901 cagagttatt tacggggcgg ttacgatggg gcagaccgtg tccggtgggc aggcgacagg
      961 tatcaccgat gggataagac tgtctgattg gtattataaa tatataagag agatcaaaca
     1021 catgagatca aatacatgag ttcatatatg tgaaataaac acggtgagtg tgatcagtgg
     1081 gcatgatgag aacgcagtag caacagactg cgctatccct gatagctggc gtataacccg
     1141 cgtatataaa acgtatctgc aatgtacgtg aacagagtgc attaagcatc cgtctgagcg
     1201 gtaaacatat ccgacctggt gagtgacagt ctgagagacc ggttaagtgg tggctcggga
     1261 gtaatgagtt tggggtgggt accagaatgc cagtctggcg gtatgctgac agtatcacgt
     1321 taattgcagc ataaagtgat gagaggctaa tggaagttaa aaccaaagca gttccgtcgt
     1381 tgttcatccc gggagacgct ggaaaaagtg atttcccaca cgcgttataa acttacctct
     1441 gcggaactgg aagccttcaa ctctgtggtt gatcactggc tggcagaact gacaatgaac
     1501 aagctttacg atcgtgttcc cgcttcagtc tggaaatatg tcatctgagc gttcggtaat
     1561 cacaactgca tcgcaccagg tgttatccgc tttgtgattc ttattggctc ttctcaggtt
     1621 attaaaagct gaatatgttc ctgttgtcgg gaaccaggat aataccggga acgttaagcg
     1681 gtatagcgac aacaaagaat tcgaaatcaa aagggaatgg tgccggaggc gtgggcggta
     1741 atgggctgat tttcaggtat taccggctta cagcgtgtgg ttgatattga ggggctgtga
     1801 gtttcgcgta ttt
//
LOCUS       DAFAVF010000315         1782 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.323, whole genome shotgun sequence.
ACCESSION   DAFAVF010000315 DAFAVF010000000
VERSION     DAFAVF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.323"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     repeat_region   72..1625
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=72..100
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
BASE COUNT          417 a          489 c          493 g          383 t
ORIGIN      
        1 tgtgatacta taaagttggt agattgtgac tggcttaaaa aatcattaat taataatagg
       61 ttatgtttag agtgttcccc gcgccagcgg ggataaaccg gcattgacgc tttaaacgac
      121 gacggacgcc acgtgttccc cgcgccagcg gggataaacc gaaaacagcc tttagattag
      181 tacctgacga ccggtgttcc ccgcgccagc ggggataaac cgtaaacgca cctggcgcgc
      241 cactttatca acaagtgttc cccgcgccag cggggataaa ccgcggcttg tttaattgcg
      301 tggaacgtct caattgtgtt ccccgcgcca gcggggataa accgacggcg tggattgagg
      361 gacgggtatt tggtccgtgt tccccgcgcc agcggggata aacctagatc gcgccacgag
      421 gaaacgaata tgaacgggtg ttccccgcgc cagcggggat aaaccgtgcc gccaggccag
      481 cgacacatca gacaactggt gttccccgcg ccagcgggga taaaccggtc tgtgatggcc
      541 tgctcgtgag tccgcggcgg tgttccccgc gccagcgggg ataaaccgtt ttgatttcat
      601 taacggcgct ccccatattt gtgttccccg cgccagcggg gataaaccgt gcgccgtagc
      661 gtgtccacct attgtagtaa agtgttcccc gcgccagcgg ggataaaccg atacaaacgc
      721 ggtgtttatc aatatgaatt ttgtgttccc cgcgccagcg gggataaacc ggcaccacgc
      781 gtaccccgat gtttgttttg ccagtgttcc ccgcgccagc ggggataaac cgatccggct
      841 atatctttga gcattacaga aatagtgttc cccgcgccag cggggataaa ccgctattgc
      901 tttcgtacag attttcagtg gtgctgtgtt ccccgcgcca gcggggataa accgaaaatt
      961 ctgtgtttcg accattactt cggtaagtgt tccccgcgcc agcggggata aaccgggggt
     1021 agaattattc ttcgtgagcg atttatcgtg ttccccgcgc cagcggggat aaaccgcggc
     1081 gttccgtgcg gcaattggaa tcacaccagt gttccccgcg ccagcgggga taaaccgcgt
     1141 tctgaatccg atattcttca gcaccttcag tgttccccgc gccagcgggg ataaaccgat
     1201 ttgggggtat gagagcgccg agccgttcgg gtgttccccg cgccagcggg gataaaccgg
     1261 ctccctgtca gttgtaatcg ataacgttga tgtgttcccc gcgccagcgg ggataaaccg
     1321 atgtaggggc aatcgaacga ttctctgccg acgtgttccc cgcgccagcg gggataaacc
     1381 gccgagcccg attatcggca tgagcgatgc ggagtgttcc ccgcgccagc ggggataaac
     1441 cgtcgaagaa gaaagggaaa taatgcgagg aacggtgttc cccgcgccag cggggataaa
     1501 ccgtattacg cgccagcaat gctgacagcg gcaaagtgtt ccccgcgcca gcggggataa
     1561 accgcgcgag agccagcaaa acgccagggc acaaaagagt tccccgcgcc agcggggata
     1621 aaccaccata taacccgtta tctctttctc aagtttttat attagcagta cttgtaataa
     1681 gcaacatatc cacgtaaccc ctcatgttca aaatagttct ccatgccaga gaagttcaca
     1741 attatcgata caaaaaatta aatttaatca aagtgttatt tg
//
LOCUS       DAFAVF010000316         1751 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFAVF010000316 DAFAVF010000000
VERSION     DAFAVF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.324"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            81..335
                     /gene="bssS"
                     /locus_tag="LGK42_004562"
     CDS             81..335
                     /gene="bssS"
                     /locus_tag="LGK42_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBK2660705.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            450..1568
                     /gene="solA"
                     /locus_tag="LGK42_004563"
     CDS             450..1568
                     /gene="solA"
                     /locus_tag="LGK42_004563"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBK2660706.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1616..1729
                     /locus_tag="LGK42_004564"
     CDS             1616..1729
                     /locus_tag="LGK42_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBK2660707.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          447 a          424 c          429 g          451 t
ORIGIN      
        1 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
       61 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
      121 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
      181 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
      241 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
      301 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
      361 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
      421 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
      481 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
      541 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
      601 gccatgctta tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt
      661 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
      721 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
      781 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
      841 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
      901 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
      961 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaaccgtt gacggtgagt
     1021 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
     1081 tgcctgtcca gcccgtacgc aaagtatttg cctggtatca ggccgatggg cgctatagcg
     1141 tgaagaataa attcccggcg tttaccggtg aacttcccaa tggcgatcaa tattatggtt
     1201 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
     1261 cagcggatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt
     1321 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
     1381 ataattcgcc tgacgaagat tttattatcg ataccctacc cgaccacgat aatacactgt
     1441 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
     1501 ccgattttgc gcaagacaaa aaaagcgatt ttgatttaac gccattcagg ctttcccgct
     1561 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
     1621 tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct
     1681 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
     1741 gacataaact g
//
LOCUS       DAFAVF010000317         1743 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFAVF010000317 DAFAVF010000000
VERSION     DAFAVF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.325"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(139..1677)
                     /gene="cadC"
                     /locus_tag="LGK42_004565"
     CDS             complement(139..1677)
                     /gene="cadC"
                     /locus_tag="LGK42_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HBK2660708.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGNLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          519 a          355 c          359 g          510 t
ORIGIN      
        1 gattgcggga tattctctgg ttaaatttat gtaataaaaa ttatgcggca aataaattgc
       61 cgcaacatat tataccaaca gaaacataca aaaactcaac aacaaatatt cccgagcata
      121 aatcaacccg ggagttactt attctgaagc gagaaatttg tcgagataag gtacaacata
      181 aggaacagaa gtctggaata taccattttc aatccagtaa agggtgtttg cccctgggcg
      241 taaattaaag gcggtgagat atgcatcagc cgcttcccgg ttcatcccct tcatttcata
      301 aaccttgcca agcaacacat aatttagcca ggacatttca agatcaatgc cagtatttat
      361 cgcctggtaa gactcatctg ttttaccttt taccagagca ctgaccgctt ttatttgata
      421 tataatggac agattattca attccggcag tgtaacaatg ttgtctattt ctgtgttcag
      481 tgctgctaat tgtttttcat ctaaaggatg ttgagaatgg cgcacgatat caactaatgc
      541 tttttctgct ctcgcgtagg taaattctgg ggatgattga acaatctcac ctaataattc
      601 actggcacgg ttcaatgatt tatcatcgcc atgcagtaaa taatcatgtg cctgataaaa
      661 attagttaat aacgcaccac gatgaggcaa aatttgctgg agcgtctcct gcattcgttg
      721 tggccacggt tggtttaacg cttttgataa actctccagt aaatcatttt gaatcgccag
      781 ctgattaccg ttagtgatga cataacgttt atccagcatg gttgaaccat ctgcattgtc
      841 taccaatttt atcgacataa agcattgttg agcacggtat tggcgctgat taacaaacgc
      901 aatagataat gttttaccgg aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg
      961 caccataaag gtggagaagg tgttaagtga tgtcgccacc aaattaccca cgcctatcgc
     1021 gtaagagagc tgatacgggg aactccagct gttacaactt ttatttacca tattaatgtc
     1081 aatatcgcgt ggattgagca aaatacgcga tttgctcatc ggaagacgtg tatcaagagt
     1141 tgaaaacgct accagtgcta cacagatacc taacgacaac aggaaaaaaa accataccca
     1201 aaaggtagtg aatcgtttgc ttttaactgg ggattgttca ggtggcgttg cggtgttttg
     1261 aatgttaaga ctgtgggagg gagaatctgt ggcaggaacc gcctctggta tagggggagg
     1321 cgaagatagc attatttcct ctccctcttc ttcgctgtac cagataaccg gcaccattaa
     1381 tttatagccg cgctttggta cagtagcgat atagacagga ctatcttcat cattatcttt
     1441 taatgactta cgtagttctg agatactctg cgtcacaaca tgattggtga caatacttct
     1501 cttccagaca ttatcgataa gttcatccct gctaagtact tcgccactgt gttgagcaaa
     1561 gaaaaccaga agatcgatta atctcggctc aagggtaagt tgacgcccat tgcggctaat
     1621 ttggtttatg gacggagtaa caagccattc gccaacgcga actacaggtt gttgcataat
     1681 agaaactcat tcgaaaaggg aatgatgcaa tgataattgc cacaacctat ttttaccatc
     1741 tat
//
LOCUS       DAFAVF010000318         1733 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.326, whole genome shotgun sequence.
ACCESSION   DAFAVF010000318 DAFAVF010000000
VERSION     DAFAVF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.326"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            124..339
                     /gene="essD"
                     /locus_tag="LGK42_004566"
     CDS             124..339
                     /gene="essD"
                     /locus_tag="LGK42_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBK2660709.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            344..655
                     /locus_tag="LGK42_004567"
     CDS             344..655
                     /locus_tag="LGK42_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBK2660710.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            652..1185
                     /locus_tag="LGK42_004568"
     CDS             652..1185
                     /locus_tag="LGK42_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBK2660711.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1182..1679
                     /locus_tag="LGK42_004569"
     CDS             1182..1679
                     /locus_tag="LGK42_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HBK2660712.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          500 a          355 c          447 g          431 t
ORIGIN      
        1 acccacattt ttaatgtgta tgttttttag cgccgttgag aacaacgtgt gctgtcaaaa
       61 ctaccccgta gactccgatc ttttcaaaca tattgcacca tccgtgtaca tcggggtgag
      121 gatatgaaat caatggataa gttaacaaca ggtgttgcct atggcacatc ggcgggtaat
      181 gctggtttct gggcattgca gttactcgat aaagtaactc cgtcacagtg ggctgcaatc
      241 ggtgtgctgg gtagcctggt ttttggcctg ctgacgtatc tgacaaatct ttatttcaag
      301 attaaagaag acaggcgtaa ggctgcgaga ggagagtaat ccaatgactc aagactatga
      361 actggttgtg aaaggagtcc gtaattttga gaataaagtt acggtaactg tagccttaca
      421 ggacaaagaa cgctttgacg gtgaaatttt tgacctggat gtcgccatgg accgtgttga
      481 aggagctgcg ctggagtttt atgaggcagc agccagaagg agcgtccggc aagtcttcct
      541 ggaagtagca gaaaaattgt cagaaaaagt tgagtcttat ctgcagcatc agtactcctt
      601 taagattgaa aatcctgcca ataagcacga gcgtcctcat cataaatatc tatgaacaca
      661 aaaatcagat acggcctgtc ggctgccgtt ctggcgctga ttggtgctgg cgcatctgct
      721 cctcagatac ttgaccagtt tctggacgaa aaagaaggca accacacaat ggcataccgc
      781 gatggttctg gcatatggac catctgtcgg ggtgccacag tggtggatgg aaaaaccgtt
      841 tttcccaata tgaaactgtc gaaggaaaaa tgcgaccagg tcaacgccat tgagcgtgat
      901 aaggcgctgg catgggtgga gcgcaatatt aaagtaccac tgaccgaacc acaaaaagcg
      961 ggtatcgcgt cattttgtcc ctataacatt ggccccggta agtgtttccc gtcgacgttt
     1021 tataagcggc tgaatgctgg tgatcgtaaa ggtgcatgcg aagcgattcg ctggtggatt
     1081 aaggatggcg gacgcgattg ccgcattcgt tcaaataact gttacggtca ggttattcgt
     1141 cgtgaccagg agagcgcatt aacctgctgg gggatagaac agtgaatcag atattcatgg
     1201 tgatttttct cgtgttgtca ggatttatcg tcggaaatgt ctggagcgac cgaggatggc
     1261 aaaaaaaatg ggcggaacgt gatgctgccg cattatcaca agaggtaaat gctcaatttg
     1321 ctgctcgaat aattgaacag gggcgaacta tagcccgtga tgaggctgtt aaagatgcgc
     1381 aacagaaatc tgctgaaatt tctgccaggg ctgcttatct gtctgatagt gttaaccagt
     1441 tgcgtgccga agcaaaaaaa tatgccatac gccttgacgc agcgaagcat accgcagatc
     1501 ttgccgctgc cgtcagaggc aaaacaacca aaaccgccga aggaatgctc accaacatgc
     1561 tcggagatat tgcagcagaa gctcagcttt atgctgaaat tgctgacgaa cgctacatcg
     1621 caggagtgac ttgtcaacag atctatgaat ctttaagaga taaaaagcat caaatgtagg
     1681 gtaatattaa atcggaacat ttacatcgcg gaatgtaaaa tttaaataaa aag
//
LOCUS       DAFAVF010000319         1733 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.327, whole genome shotgun sequence.
ACCESSION   DAFAVF010000319 DAFAVF010000000
VERSION     DAFAVF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.327"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            261..1544
                     /gene="gltA"
                     /locus_tag="LGK42_004570"
     CDS             261..1544
                     /gene="gltA"
                     /locus_tag="LGK42_004570"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBK2660713.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          460 a          416 c          394 g          463 t
ORIGIN      
        1 tgtggaagta ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt
       61 cggattgcta agtacttgat tcgccattta ttcgtcatca atggatcctt tacctgcaag
      121 cgcccagagc tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa
      181 acgggtaaag ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgtaata
      241 aggcgctaag gagaccttaa atggctgata caaaagcaaa actcaccctc aacggggaca
      301 cagctgttga actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta
      361 ctctcggttc aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg
      421 aatctaaaat tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga
      481 tcgatcagct ggcgaccgat tctaactacc tggaagtttg ttacatcctg ctgaatggtg
      541 aaaaaccgac tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga
      601 tccacgagca gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag
      661 tcatgtgtgg tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca
      721 atcctcgtca tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg
      781 cgatgtgtta caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct
      841 atgccggtaa cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc
      901 cgattctgga acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg
      961 cctctacctc caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg
     1021 cagcaggtat tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga
     1081 aaatgctgga agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag
     1141 ataaaaatga ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc
     1201 cgcgcgccac cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag
     1261 atgacctgct ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta
     1321 tcgagaagaa actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg
     1381 gtattccgtc ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg
     1441 cccactggag cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata
     1501 caggatatga aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt
     1561 tgtaaatatt ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa
     1621 aaattgtaaa aataaaatcc actatcggac attcatatat tctaaaaaaa aatatatcgg
     1681 tataaattat aaacaaatcg atgattttta atcatctatc ttcctctcag tta
//
LOCUS       DAFAVF010000320         1712 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFAVF010000320 DAFAVF010000000
VERSION     DAFAVF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.329"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            12..1380
                     /locus_tag="LGK42_004571"
                     /pseudo
     CDS             12..1380
                     /locus_tag="LGK42_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT          479 a          432 c          409 g          392 t
ORIGIN      
        1 atgacgccgg aatgttcacc gtgaaagaaa tcaatcgcct caaaatcctt caggatgtca
       61 tcgatcgcaa tatccgtcct ggacaggctg ccgaaatgct gggcatcacg ccacgtcatt
      121 gcagccgtct gctcaaacgt tatcgccagt atgggccgct tggcatgaac aaccagagtc
      181 gtggccgcac tggtaaccgt ctgttgccag cgtcactgac agatcaggca ctcagtataa
      241 tccgggagca ctaccgtgat tttggcccca cgctagcgcg ggaaaagctg gaggaagttc
      301 acggtctggt ccttggaaag gaaaccatcc gacgtctcat gataaaggcc ggactctgga
      361 ttccccgcag acaacgcgca ccgaaaattc atcagcctcg ttaccggcgt ccttgtactg
      421 gcgagctgat acaaattgat ggctgcgacc accactggtt tgaaaaccgg ggtcgaccct
      481 gttctgccct ggtctatgtt gacgatgcca ccagtcgcct gatgcacctg ctgtttgtga
      541 aatctgagtc cacatttacc tattttgaag ctacacgtgg ctatatcgag aaatacggta
      601 aaccgatgat cctctacagc gataaagcca gcgtattcag agtcaataat aagcacgcca
      661 ccaccggccc cggcgaaacc cagtttgcca gggccatgcg ctgccttaat attactcctt
      721 tgtgtgctga aaccagccag gcaaaaggcc gcgttgaacg tgctcatttg actcttcagg
      781 accgactcgt taaggagctc aggcttaagg ggatctgtac aatagaagcg gccaatgcat
      841 ttgcagaagg attataaccg gcgctttgcc aaagaaccct tgcatgattt taacgcgcac
      901 cggccgctgg cattagacga tgaccttgat gcagaattta cctggcgaga gcctcgccgg
      961 gtatcaaaaa atctgactgt acaatacgat aaaatgctct atctgattga ggacaacgag
     1021 tacagtcgaa gagccatcgg caagtatatt gatgtctggc attatcctga cggacataag
     1081 gagctgcgac taaatggcgt gctgctgccc tactccacct atgaccggct ttctgaggtt
     1141 gatcccgtcg ccattgtaga taacaagaga cttggccatg tgttggatgt tgcccgtcag
     1201 gtccagagaa aacgggacaa taatcgctca caatcgttac cttgttctgg tgatgagcct
     1261 tcccgcagac ggcacgcccc tagcatcaac aaatctcagc gctcactgaa cgaagatgac
     1321 ctacttgaag ccatgataaa gcttcaggga agctctgagg ccatctttgg taaaaggtga
     1381 acttggcgga aacctcgtac taactgacca acggcgatgt gtgcgctatc tttcggctat
     1441 tgcaatcaaa gccagagaag atttctgaga aatgcaaata acttcaatga tattatatac
     1501 aaaggaaacc gaggtaaaat tcgggtgtca cagaaccccg aaccgcttcg cggtgcaaag
     1561 agccaaacag caagtatcac agaacagcat gtaaaaaaat cttgaggcta tttcatggca
     1621 attattaata tttattctaa gagacaaaga aaaattcgtg gcgaagtaaa tgacgtatat
     1681 caatataaca atattccaca tgcactcagg gt
//
LOCUS       DAFAVF010000321         1700 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFAVF010000321 DAFAVF010000000
VERSION     DAFAVF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.330"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..1615
                     /locus_tag="LGK42_004572"
     CDS             <1..1615
                     /locus_tag="LGK42_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2660714.1"
                     /translation="GMLEKAEKAFDEADKKWQWYQSRSHRRGKTSAFLANLRGAWEDR
                     ANAQLGLSAATLQADLEKAREMAAKDWAESEASRLKYTEEAQKAYERLQTPLEKYTAR
                     QEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALL
                     TLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDE
                     TLEYKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIEAKNRGLTDRQAAREA
                     TEQRLKEQYGDNSLALNNVMSEQKKTWAAEDQLRGSWMAGLRSGWSEWEESATDSMSQ
                     VKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAI
                     GGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRI
                     GVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNGLPGPQMMK
                     AVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            1612..>1700
                     /locus_tag="LGK42_004573"
     CDS             1612..>1700
                     /locus_tag="LGK42_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660715.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYS"
BASE COUNT          437 a          397 c          571 g          295 t
ORIGIN      
        1 gggaatgctg gagaaagcag aaaaggcttt tgatgaggcg gacaaaaaat ggcagtggta
       61 tcagagccga agccaccggc gcggtaaaac ctcagcattt cttgccaatc tccggggagc
      121 atgggaggac agagcgaatg cgcaacttgg gctttcagcc gccacgttgc aggccgatct
      181 tgaaaaggcc agagagatgg cagcaaagga ctgggccgag tctgaggcat cacggctgaa
      241 atataccgaa gaggcgcaga aggcttacga acgcctgcag acgccgctgg agaaatatac
      301 cgcccgtcag gaagaactga acaaggcact gaaagacggg aaaatcctgc aggcagatta
      361 caacacgctg atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa agccgaaaca
      421 gtccggcgtg aaagtgtctg cgggcgatcg tcaggaagac agtgctcatg ctgccctgct
      481 gacgcttcag gctgaactcc ggacgctgga gaagcatgcc ggagcaaatg agaaaatcag
      541 ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc gcggtactgg aggaggcggc
      601 gcaacgtcgc cagctgtccg cacaggagaa atccctgctg gcgcataaag atgagacgct
      661 ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg agcgcctgaa
      721 cgcgctggcg cagcaggcgg ataagttcgc acagcaacaa cgggcaaaac gggcagccat
      781 tgaggcgaaa aaccgggggc ttactgaccg gcaggcagcg cgggaagcca cagaacagcg
      841 cctgaaggaa cagtatggcg ataattcgct ggcgctgaat aacgtcatgt cagagcagaa
      901 aaagacctgg gcggctgaag accagcttcg cgggagctgg atggcaggcc tcaggtcagg
      961 ctggagtgag tgggaagaga gcgccacgga cagtatgtcg caggtaaaaa gtgcagccac
     1021 gcagaccttt gatggtattg cacagaatat ggcggcgatg ctgaccggca gtgagcagaa
     1081 ctggcgcagc ttcacccgtt ccgtgctgtc catgatgaca gaaattctgc ttaagcaggc
     1141 aatggtgggg attgtcggga gtatcggcag cgctattggc ggggctgttg gtggcggcgc
     1201 atccgcgtca ggcggtacag ccattcaggc cgctgcggcg aaattccatt ttgcaaccgg
     1261 aggatttacg ggaaccggcg gcaaatatga gccagcgggg attgttcacc gtggtgaatt
     1321 tgtcttcaca aaggaggcaa ccagccggat tggtgtcggc aacctgtacc gcctgatgcg
     1381 gggctatgcg gaaggtggtt atgtgggcgg tgccggaagt ccggcgcaga tgcggcgggc
     1441 tgaaggcatt aattttaatc agaacaatca cgtggtgatt cagaacgacg gtacgaatgg
     1501 tctgccaggt ccacagatga tgaaggcagt gtatgacatg gcccgcaagg gtgcccgtga
     1561 tgaaattcag acacagatgc gtgatggtgg cctgttctcc ggaggtggac gatgaagacc
     1621 ttccgctgga aagtgaaacc cggtatggat gtggcttcgg ccccttctgt aagaaaggtg
     1681 cgctttggtg atggctattc
//
LOCUS       DAFAVF010000322         1670 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFAVF010000322 DAFAVF010000000
VERSION     DAFAVF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.331"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..399
                     /locus_tag="LGK42_004574"
     CDS             <1..399
                     /locus_tag="LGK42_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660716.1"
                     /translation="PFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            475..930
                     /locus_tag="LGK42_004575"
     CDS             475..930
                     /locus_tag="LGK42_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660717.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            1009..1242
                     /locus_tag="LGK42_004576"
     CDS             1009..1242
                     /locus_tag="LGK42_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBK2660718.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            1342..>1670
                     /locus_tag="LGK42_004577"
     CDS             1342..>1670
                     /locus_tag="LGK42_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HBK2660719.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTS"
BASE COUNT          430 a          432 c          418 g          390 t
ORIGIN      
        1 ccatttggtg cactgacagg gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc
       61 gctgtatcag gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa aattctggca
      121 atgattcagg agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg acatgagatt
      181 gaaaatctga acgagaatct ggcccgtgtt atcgatcaga atgctctgga gatcattttc
      241 gccgccggta tacaacaacg ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt
      301 atcatcaggc aacgccccgt catggaatat gatgcatccc atctcggcaa tatggttaac
      361 cgactggaag aagcattacc cccggagtta ccggcataac ggctattctg cgccttttac
      421 gctttatcac ctgatttgtc agtgtcatcg tcatgactca gagaggtaaa taccatgatt
      481 cacctgttca aaacctgcat gattaccgcc ttcattctgg ggttaacgtg gtctgcccca
      541 ctccgggcac aggatcaacg ttacatcagt atacgcaata cagatacgat atggctcccg
      601 ggaaatattt gtgcttacca gttccggctg gataatggcg gaaacgatga aggatttggc
      661 cccctcacca tcactctgca actcaaagac aaatatggtc agacgctggt gaccagaaaa
      721 atggaaacgg aagcttttgg tgacagtaat gccacgcgaa ccacagacgc atttctggaa
      781 acggagtgcg tggaaaatgt cgccacaacc gaaatcatca aagcaactga agaaagtaac
      841 ggccatcgtg tcagtctgcc gttatcggtt ttcaatcccc aggactacca tccactgctg
      901 attaccgttt ccggaaaaaa cgttaactga tccctgaagt cagtttcatg cttcagcctc
      961 tgtcacaacg cgtggcagag gctttttatt tatcaggaga aaacagatat gccgggttac
     1021 acagagtatg tcctggctga aggatctttc tcatacgggc aggcggttgc tgtcataacc
     1081 gcttaccgca atgtgtttat ccaggatgac ccgggaatgc atttccgtcg ggttatccgc
     1141 aacgcggaag gtcagcgacg ctggcgctgc cggaattctg aacccgatgc cggaaaggtg
     1201 cttaatacgc ggctcgccag tgacggtctt ctcagacaat aaacgtcatt catttcaccc
     1261 atcaggccgc gtcttctccg ggagacgtgg ctttttcatt tattacacca gtaaatctta
     1321 accaccgata aggagcaaat tatgcgacta gccagtcgtt ttggttatgc gaaccagata
     1381 cgccgtgacc gtccgctgac acacgaagaa ctgatgcact atgtgccggg gattttcggg
     1441 gaagataaac acacgtcccg aagccagaac tatacgtaca tccccaccat caccgtactg
     1501 gaaagcctgc agcgggaagg ctttcagcca ttcttcgcct gccagacccg tgtgcgcgac
     1561 ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg agagataaac
     1621 ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc
//
LOCUS       DAFAVF010000323         1664 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFAVF010000323 DAFAVF010000000
VERSION     DAFAVF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.332"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(72..395)
                     /locus_tag="LGK42_004578"
     CDS             complement(72..395)
                     /locus_tag="LGK42_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBK2660720.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            659..745
                     /gene="azuC"
                     /locus_tag="LGK42_004579"
     CDS             659..745
                     /gene="azuC"
                     /locus_tag="LGK42_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBK2660721.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            860..1111
                     /locus_tag="LGK42_004580"
     CDS             860..1111
                     /locus_tag="LGK42_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBK2660722.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1190..>1664)
                     /locus_tag="LGK42_004581"
     CDS             complement(1190..>1664)
                     /locus_tag="LGK42_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBK2660723.1"
                     /translation="NSQMNREFYASNLYLHLSNWCSEQSLNGTATFLRAQAQSNVTQM
                     MRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLAQLADEAKELN
                     DDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLNVVSHQLH"
BASE COUNT          464 a          374 c          398 g          428 t
ORIGIN      
        1 atacgaatgg gaataaaacg cattaccgct atttctttaa tcagcgaaga tcccctcgct
       61 gattaaaata tttagagaaa gacagggaag gcggaatgat ggcactgata tgacaaagtg
      121 aattccgtat tcaaacataa ggaacccgca aaaaggctac agttgcctac tggctgacca
      181 aaggggaccg cgtgggtata gccttcctgc tggcatgcgc gatcggcgat cccgcgactg
      241 acataacgat cggtatgggc atgctgcaaa aaagcctggt cataaactaa ccgtacaata
      301 cctgttgccg cgtcaacttc gctcacatgg gcctggcgag taaccgtaca ccccgccagc
      361 gttattaatg aaagagtcag aatcagaagt ctcatggtcc agccagaaga gtaagagaaa
      421 gcactatttt acgcgttaca gtgctgcgac aatggccgga aaagacgcct ggcaggcgct
      481 atttcttgcc atctgtaggt gtcagaaata gggggggctg ttttttgatt atcattttta
      541 taacattaaa ggggtattta tgtgatttgt atcacataaa gattgatttt tgtgatggtt
      601 gtcatattat aagcgcaaag acaataacac ctgtataaca aatggtcgga gtgccgcgat
      661 gaaactgcgc aaaatcctga aaagtatgtt caataactat tgcaagacgt tcaaagacgt
      721 accgccaggc aatatgttcc gataacaaaa aacctgctcc ggcaggtttt tttgtgtcct
      781 gatgacggtg gatgtgacta tcgctgaaga tgtgaagccg ctactataac ggctttcaaa
      841 atgctatcag gagtttacga tgtcccagcc gctgaatgcc gatcaggaac tggtttctga
      901 tgtggttgct tgccagttgg ttatcaaaca aattctggat gttctcgatg taatcgcgcc
      961 cgttgaagtt cgtgaaaaaa tgtccagtca gctgaagaat atcgacttta ctaaccatcc
     1021 cgccgccgca gacccggtca ccatgcgggc aatccaaaaa gccatcgcgc ttattgaact
     1081 caagtttacc ccacagggtg aatcccatta aaaaaaacgc tgcttcaaac agatgaggca
     1141 gcggtttctc aaaaccttca tcggcgcaat gcattagcgc cgatgatgat caatgcagct
     1201 ggtgtgacac gacattcaga acatgttggt cggtctgcac agggcacata cccgcaagtt
     1261 tcgcactgcg cacttcatca agaatggttt gcagcaacag accatcatgc tgctgttctt
     1321 tttccagatc gcgcaggaaa ttgacggttg aatcatcatt cagttctttc gcttcatcgg
     1381 ctaactgtgc caacgtacta gaacgttgct cgtattcttc catcgttttt tggaacagtt
     1441 cttccagaga gttcagtttt tcaccgggaa catcaatggc tttaacgatg ggggtagcgc
     1501 cgacactctt cataaagtta aacatgcgca tcatttgggt cacattactc tgtgcctggg
     1561 cgcgaaggaa agtggcggtg ccgttcagac tctgttcaga acaccagtta ctcaggtgaa
     1621 ggtagagatt ggatgcgtaa aactcgcggt tcatttgaga gttg
//
LOCUS       DAFAVF010000324         1663 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFAVF010000324 DAFAVF010000000
VERSION     DAFAVF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.333"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..1068)
                     /gene="pilV"
                     /locus_tag="LGK42_004582"
     CDS             complement(<1..1068)
                     /gene="pilV"
                     /locus_tag="LGK42_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBK2660724.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAIL"
     gene            complement(1068..>1663)
                     /locus_tag="LGK42_004583"
     CDS             complement(1068..>1663)
                     /locus_tag="LGK42_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK2660725.1"
                     /translation="SIIGRTLLFRVRYFLSDTGHLWYKTHPAVLSGIWLYSIAVALII
                     LSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFTRRFLIAGMLSQITTDIWWFRT
                     TEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITAWSGLYNAIWCVLLGTGGFVLW
                     HSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI"
BASE COUNT          454 a          410 c          352 g          447 t
ORIGIN      
        1 aagtattgcc cctgtattat cgcggcctac aaggccgtta ggcgaacatg atgcgccagc
       61 aacggcagtt ctttccagtt gtaagtattc accagtataa aggcgaccat cagcccgaac
      121 agtaccgcct ttcacctgac cgccggtata gatgcccttg ttgtttacac ttcgaaccca
      181 tgatccatcg gacatataaa atccgccacc gtgagtttca ttgagccagc ctttactgtt
      241 acgagtaatt aaccaaccat tattacttct gatatcaccg ccagcggtga catttacgtc
      301 aaagtttccg ctattgcctt ttacctgacc gctgaaagtg ccatttacac cattcacatt
      361 gccgctgaaa ttacctgtct gggcatttac tgccccaacg ttattcaggt tatttgatcc
      421 catatcaatg gccgtgtgca ttttgtttaa gtcagggcga ccattgacct ggaatctgta
      481 aagacgatca gtgtcctcag ctgcaccact aagttcatct gtcgataaca atacggcaat
      541 atgcccgtta ccgcttttgg caccataatt acttaaagct actgaccagg aacgtaatgc
      601 acctgtggct gttttgccgt cctggatata tccaccaaga ccagtggtaa tatccttagc
      661 catctggata agtgctttca ctggataagg tgtgccacca ctggatacaa ccattgcctg
      721 tagtaattcc gggttttggg cgtttcgaac cacatatgcc tgtaaccgct gcccctcgct
      781 gtttgtctca gtaaacccgc tggacaaaaa gccggtattt ttcagcatgg ttgtcgtgat
      841 aacggcagga gttgttgtgg tactactgcc ctggagagtc gtgtagtttt tccctatata
      901 agagcgggcc gcactggtcc agttgctgac aagacgtgct tcagtttgcc atccttttgt
      961 ttgaatatag tcctgccata tgcccgctcc ccaggcaatc aggagcataa caatcagtaa
     1021 agccgcgcca gtttccagcg atgcccagcc tcggtcatat tttttcatca gattacccat
     1081 agtggttgat aaagattatc cagcagcaaa aggacatggc caaaacatag ccatggaccc
     1141 agagggcctt ctttatgccc ttttatgcac catgttgaat gccataagac aaatcctcct
     1201 gtgcccagca acacacacca tatggcgtta tataaccctg accatgcggt aatacctgca
     1261 ataagccaca ggtctccagt gccaatatta agacgatggc ggttaactaa tttatgaagg
     1321 cagaataaga caattgcagc agtggcaaat tctgttgtac ggaaccacca tatatcagtc
     1381 gtgatttgtg atagcattcc tgcaattaaa aaacgtcggg tgaatgttcc tggtaataaa
     1441 cctgtcagtg catccgttac cgccatttgc agtatgaacg aaagaataag gacagcatga
     1501 atacggtaga gcagtggtga tgatgataaa ataattaacg ccacagctat actgtatagc
     1561 catattcctg acagaacagc agggtgtgtc ttataccaga gatgtcccgt atcagataaa
     1621 aaataacgaa ctctgaatag cagagttcgt ccaataatgg aaa
//
LOCUS       DAFAVF010000325         1632 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFAVF010000325 DAFAVF010000000
VERSION     DAFAVF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.334"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            2..580
                     /locus_tag="LGK42_004584"
     CDS             2..580
                     /locus_tag="LGK42_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBK2660726.1"
                     /translation="MFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVA
                     SIKGYSNHEKDVLEAVTLARSQANRASSDLQQTPGNEQKLQAWQQAQAQVTRTLGQLT
                     IISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKV
                     MDYTPKKNYLPDDVAAVSKAPTIDFSQNASAH"
     gene            594..1499
                     /locus_tag="LGK42_004585"
     CDS             594..1499
                     /locus_tag="LGK42_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBK2660727.1"
                     /translation="MKNLILLLVLICSSAFAVQVPVPEFAKYINDLTGTLTREEVSTL
                     TSQIKTLTQKSHAQLIVLVVETTGDETIEQYATRVFERWQPGHKNLDDGILLVVAWQD
                     HTVHIEIGYGLEGILTDAQSGQIIRNSIIPAFKKGDLAVGLQQGIGDIETRLSENDLV
                     NNTSPDHPLPFSGWWALLVWAIVLTFISARGYVKTLGVICFAAIILAFVLPIAGYSGS
                     WGVLATLLCFATPFLVVAIAFTPFGRKVRDSMRSANRPSRYERSNSASRHSSSSSSSS
                     SSSFNNDNFSGGGGSSGGGGASGRW"
BASE COUNT          468 a          343 c          372 g          449 t
ORIGIN      
        1 tatatttatt ttcaatttat caggctgtgg atataacgat attcaaacct atgatgagca
       61 ggttaacgcc tcctggtctg aggtattaaa tcaatatcaa cgtcgcgccg atcttattcc
      121 taatcttgtc gccagtatta aaggttattc caaccatgaa aaagacgttt tggaagccgt
      181 aacattggct cgtagccagg ctaatcgcgc cagcagcgat cttcagcaaa ctccgggaaa
      241 tgaacaaaaa ttacaagcct ggcaacaggc tcaagcgcag gtgactcgca ctctcggtca
      301 gttaacgata attagtgagc gttaccctga gttaaaggct caagagctct atcaaaactt
      361 aatggtacag ctcgaaggaa gtgaaaatcg aattgccgtt gcgcgaggaa gatatattaa
      421 agccattgaa caatataatg ttactatccg caaattcccc gctgtattaa ccgcaaaggt
      481 catggattac acaccgaaga aaaattattt gccggatgat gtcgccgctg tgagtaaagc
      541 accaacaata gattttagcc agaatgctag tgctcattag gcgagttaat tatatgaaaa
      601 atttaatctt acttcttgtt ctgatctgtt catctgcgtt tgcagtgcaa gttccagtac
      661 ctgaattcgc taagtatata aatgatttaa cagggacatt aaccagggag gaagtaagta
      721 cgctgaccag tcagattaaa accctgacgc aaaaaagcca cgcacaactg atcgtgttag
      781 tagtagaaac aacaggcgat gaaaccatcg agcaatatgc aacacgagta ttcgagcgtt
      841 ggcagcccgg tcataagaat cttgatgacg gtattttact ggtagttgcc tggcaagatc
      901 atacagtaca tattgaaatt ggctacggac ttgagggtat tctgacggat gcgcagtccg
      961 ggcaaattat acgcaacagt attattcctg cgtttaaaaa gggcgatctt gctgtcggct
     1021 tgcaacaagg tattggtgat attgaaaccc gattatcaga aaatgattta gtcaataata
     1081 catcgcccga tcatccttta ccctttagcg gttggtgggc tctgttagtc tgggcgatag
     1141 tactgacgtt tatttctgcc agaggatatg tcaaaacgct gggagtgatt tgctttgctg
     1201 ccataatact ggcctttgta cttcccatcg ctggatatag cgggagctgg ggagttcttg
     1261 ccacattatt atgtttcgcc actccattcc tggtcgtagc tatcgctttc acgcctttcg
     1321 gcagaaaggt gcgtgattca atgcgtagcg ccaaccgacc gtcacgttac gaaagaagca
     1381 actcggcctc ccgacattct tcatcatcgt catcttcctc ttcatcctct ttcaacaatg
     1441 acaatttcag cggtggtggc ggttcatccg gcggtggcgg cgcgtcagga agatggtaag
     1501 acttaaaaac agattacagg gaaggtaagg aaatatgagt aagattttag cgaatcatcc
     1561 acgattaagt aaatttgtat ttggcttatt cggtgctgct ggaatgattt ggcttaataa
     1621 agccttccag gg
//
LOCUS       DAFAVF010000326         1616 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFAVF010000326 DAFAVF010000000
VERSION     DAFAVF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.335"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            104..364
                     /locus_tag="LGK42_004586"
     CDS             104..364
                     /locus_tag="LGK42_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660728.1"
                     /translation="MDQEMTFSFSYEQLTRFAEKRIRECNLDSQGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            361..951
                     /locus_tag="LGK42_004587"
     CDS             361..951
                     /locus_tag="LGK42_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660729.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRSGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
     gene            complement(969..1316)
                     /locus_tag="LGK42_004588"
     CDS             complement(969..1316)
                     /locus_tag="LGK42_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660730.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPSWDDV"
BASE COUNT          429 a          408 c          443 g          336 t
ORIGIN      
        1 attaaaaatg aaacaatatc taatattatc agaaatcaag agtacataaa tcatacagga
       61 tatgatttta catgtcagaa cattatcaga tggagcgtgc aggatggatc aggaaatgac
      121 gttttcattc agctatgaac aactgacccg gtttgcggaa aaacgcatcc gggaatgcaa
      181 tctggacagc cagggcgtaa cttatctctg cgagtcagcc aaagccggag ccgtgctgat
      241 cttctggcat gaactggcca taaatggcta tacctcaatg aatgccataa agagacagga
      301 gatcattgac gccgaccatc agcgcctcag aaagctcatc tggccggagg atgactggaa
      361 atgactcaga gccgccgacc gtcgccactg cagcggagag tgctgattgt gctggctgcc
      421 cttgatgaaa aacggtcagg acccgtgctg acgcgggaca tagaacgggt gctggagcga
      481 agcggggaag ccccggtcta cgggccaaac ctgcgcgcct cctgccgtcg tctggaggat
      541 gccggctggc tgcgtaccct gcgcgctccg aacctgcagc tggcagtgga gctgacggac
      601 gccggccggg ccgtggcaca gcctctgctg ctggcagaac aggaccgcat ccgggcagaa
      661 cagcgtgcgg cagaagtcgt cgtactgccg ctggtcccgg cagcaggtct gcccgcaggc
      721 ggcacttctg ccactgatct ggcggtggag ctgaacggca taacgtatca ggcctgccgt
      781 ggggattttg tggtgcgtct cgacggcagc acctgtctgc agttatggaa taaagaaggc
      841 agagtggtac gccgggaggg cgatccgctg gaagtggcgc agtggctgca ggcctgtcat
      901 gatgccggaa tagaagttcg tgtacaggtt aatgaaagcg tcactccgta aaaacatgcc
      961 agcacggttt acacgtcatc ccatgatggc agccggttaa ctttcctgcg ccaccagtgg
     1021 aaagaggcca tgagcagtcc gaacaaaaaa ccacccgtga tacttataat aatggcctca
     1081 cctgccacca ttcctgacgg cccccagtag ataaaccaca ttgcacatcc ccaggaaatg
     1141 ccccataagc tccccatcaa cagcgtgacc tgccagaacg gcataaaggg taatggcgga
     1201 agccggatac ccagcctcca gagaatacgc agaagaggag gggcataatt gctccgccac
     1261 atctttttgc tgtccatcag ggcaatggcc cgggcttttt tctgctcaaa ggtcacagga
     1321 gcacctccgt tccatgatgt aaacgtgaca cagcttattg ttattgatta ttattatcat
     1381 gctgtgatta tgttgtcatg caccggtaca ctgacaaaag aaagggagaa caggaggctg
     1441 gctgataaat tttaatccgc gtaagcgggc cgtgagggcc gcttacaagc gtgtatgaga
     1501 tcagtttgga tattcgaaat tattgtacgt ttaacgcacc atacattaat gctaatgtat
     1561 gcatacatta gcattaatga tgaaggaaaa gaataacaat ggcagtcaac ccactt
//
LOCUS       DAFAVF010000327         1616 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFAVF010000327 DAFAVF010000000
VERSION     DAFAVF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.336"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..180
                     /locus_tag="LGK42_004589"
     CDS             <1..180
                     /locus_tag="LGK42_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HBK2660731.1"
                     /translation="LFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKP
                     KLDLTNTDWIYGVDL"
     gene            177..878
                     /locus_tag="LGK42_004590"
     CDS             177..878
                     /locus_tag="LGK42_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBK2660732.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGATV"
     gene            875..1143
                     /locus_tag="LGK42_004591"
                     /pseudo
     CDS             875..1143
                     /locus_tag="LGK42_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145928.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
     gene            1195..>1616
                     /locus_tag="LGK42_004592"
     CDS             1195..>1616
                     /locus_tag="LGK42_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660733.1"
                     /translation="MRKFILAFVISASFTANAGVEKLGPWITKSEINKMTDQTDFVAL
                     NLSPDSYNKAGTDRATSLVLRCSDNKTDAYLSFNDYMGSDNPRITVRLDGGKPVKSVW
                     GGGEGGDSAFAPQPIQFIKTLAKHKKAIFGFEPYGSTM"
BASE COUNT          455 a          357 c          439 g          365 t
ORIGIN      
        1 ctgttccgct gggcatgcca ggacaacttc tggtccggta acgtgctgag tccggccaaa
       61 ctccgcgaca agtggaccca gctcgaaatc aaccgaaaca agcaacaggc tggcgtgaca
      121 gccggcaaac caaaactcga cctgacaaac acagactgga tttacggggt ggatctatga
      181 aaaacatcgc cgcacagatg attaactttg accgtgagca gatgcgccgg atcgccaaca
      241 acatgccgga acagtacgac gaaaagccgc aggtacagca ggtagcgcag atcatcaacg
      301 gtgtattcag ccagttgctg gcaactttcc ctgcgagcct ggctaaccgt gaccagaacg
      361 aactgaacga aattcgccgc cagtgggttc tggctttccg ggaaaacggg atcaccacaa
      421 tggaacaggt taacgctgga atgcgcgtag cccgtcggca gaatcgacca ttcctgccat
      481 cacccgggca gtttgtcgcc tggtgccggg aagaagcatc cgttaccgcc gggctgccaa
      541 acgccagcga gctggttgat atggtttacg agtattgccg gaagcgcggg ctgtatccgg
      601 atgcagagtc ttatccgtgg aaatcaaacg cgcactactg gctggttacc aacctgtatc
      661 agaacatgcg ggccaatgcg ttgactgacg cggaattacg gcgcaaggct gccgatgaac
      721 tgtcctgtat gaccgcacga attaaccgtg gtgaggctat acctgaacca gtaaaacaac
      781 ttcctgtcat gggcggtaga ccacttaacc gggctcaggc tctggcgaag atcgcagaaa
      841 tcaaagcgaa gttcggactg aaaggagcaa ctgtatgacg ggcaaagagg caattattca
      901 ttatctggag acgcacaaga gcttctgtgc gccggacgtt gctgcgacaa caggtgtgac
      961 attaaccagc ataaataagg ctgcggcaaa aatgacgcgg gcaggaatcc tggtcattga
     1021 tggtaaggtc tggcgaacgt ttgtttaacg gttagctact caggatgata gggcggggca
     1081 agtgagtatg aagcggattt tcaggaatgc catcagagtt tggaaataaa gtgggttttc
     1141 tagtggcaag agacttgata atatttagtt ctttgaatcc aaggagatag ggttatgaga
     1201 aaatttattt tagcctttgt tataagtgcc tcgtttacag caaatgctgg tgtagagaag
     1261 ttagggccgt ggataacaaa gtctgagata aataaaatga ctgaccagac tgactttgtg
     1321 gctcttaatt tatcaccaga ttcatataac aaagcaggta ctgatcgtgc aacttcactg
     1381 gtgttgcgtt gtagtgataa caaaacagat gcctatttat cattcaatga ttatatgggt
     1441 tcggacaacc caagaattac agtgcggtta gatggcggaa agccggtcaa gagtgtttgg
     1501 ggaggtgggg aaggcggtga ttctgcattt gctccacaac caatacaatt tataaagacc
     1561 ttggctaagc ataaaaaagc tatttttggg tttgaacctt atggatcaac tatgca
//
LOCUS       DAFAVF010000328         1591 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFAVF010000328 DAFAVF010000000
VERSION     DAFAVF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.338"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(136..789)
                     /gene="ribB"
                     /locus_tag="LGK42_004593"
     CDS             complement(136..789)
                     /gene="ribB"
                     /locus_tag="LGK42_004593"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBK2660734.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(893..1041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1163..1453
                     /gene="ubiK"
                     /locus_tag="LGK42_004594"
     CDS             1163..1453
                     /gene="ubiK"
                     /locus_tag="LGK42_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBK2660735.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          454 a          403 c          353 g          381 t
ORIGIN      
        1 agaatgcctg atattaatga aattttctca tagaaagaga agcggaatta acatcttgca
       61 tagcagggca aaataaaaac cgaagccagc tacaacttcg gtttcttgat taaggcagta
      121 attaagcagc ggttttcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc
      181 ttcaatagtc acgagcgcca tattgtgttt attggcaaac tcaatgcact ctggtgcacg
      241 cgccatcgtg ccatcgtcat tagtcaactc acacagcaca ccagccggtt taaagcctgc
      301 cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta caccacctgc
      361 ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc
      421 ggcaatcgct gcgcgaacgg tcgtaattcg atcagcggca gaaacaccgg tagtcacacc
      481 ttcagccgct tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac
      541 catcattggc agatcaagtt gtttacggcg atcttcagtg atgcacaggc aaacaatacc
      601 gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc atggtttctg ccgggaagat
      661 catatcgcct tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg
      721 cagcgcagcc agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt
      781 ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag
      841 gagtggcggc atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc
      901 tctcccatcc ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag
      961 ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt
     1021 cgccccgccc tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca
     1081 tagcttcaca taattctggt ttatgactta cccttatcgc actacaatgg cactcaacac
     1141 ctatcagtac agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca
     1201 ggttcacgaa tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat
     1261 ccgccaaacc ctacaagctc agctgacgcg cctcgacctt gtaagccgtg aagagttcga
     1321 cgtccaaacg caagtcctgt tacggactcg tgagaaactg gcgctgcttg aacaaagaat
     1381 gagcgaactg gaaaatcgca gtactgagat taaaaagcag ccagatccag agactctccc
     1441 cccaacgctg taaacatttg tctttaatgc actgtaaatt atgaattaat cttctaattt
     1501 acagtgcttt ctattacctt atcgcttaat tccattcaga atatcatcac aaaaatgaat
     1561 gagtgcaatt gaaaatataa ttgttatgta t
//
LOCUS       DAFAVF010000329         1590 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFAVF010000329 DAFAVF010000000
VERSION     DAFAVF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.339"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(40..>1590)
                     /locus_tag="LGK42_004595"
     CDS             complement(40..>1590)
                     /locus_tag="LGK42_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2660736.1"
                     /translation="LREHAGYHGGGSGFGGQLRAWNPPSESVDAALLPNFTRGNARAD
                     DLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFA
                     EDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTSSSRLFRTQFRMVSPKRI
                     SNPNNTGDSRNCRAGVQLNDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIH
                     VFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMD
                     FILGANSQEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGY
                     SVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQAS
                     QMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVML
                     IEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEE
                     EKSDSRAA"
BASE COUNT          325 a          506 c          417 g          342 t
ORIGIN      
        1 agcggctcat taaaagccat gctggcaata tgcgggagat tacgcagctc tgctgtcact
       61 cttctcctcc tctgttgatt gtcgcagccc ggattcaaat gctgcagccg cccaggcggg
      121 cggtttaaga ccggctgcgc ggcgctccat cgtttcacgg acctgctggg caaaaatttc
      181 ctgatagtcg tcaccgcgtt tcgcgcactc tttctcgtag gtgctcagtc cggcttctat
      241 cagcatcacc gcttcctgaa cttctttcag accatcgatg gccatacgac cggagcctat
      301 ccagtcgcag ttcccccagg cactgcgggc ttcctgaaaa ctgaagcgcg cttttgaagg
      361 taacgtcacc acgcggcgaa cgatggcctc ttccagccag cacagaaaca tctggctcgc
      421 ctgacgggat gcgacgaatt ttcgccgccc cataaagtac gcccacgact cgttcgcact
      481 ggcccgtgcc gtggagtagc tcatctgggc gtaattccgg gaaagctgct catacgagac
      541 acccagcccg gcagcgatat accgcagcag tgactgctca aacacggagt agccgttatc
      601 cgtatcctga gccgtctgca ggttcagtga gtcacccggc atcaggtgag gcacttttgc
      661 gcctcccagc cggaccggtg ctgcggcgta atacgcggca atttcaccaa tccagccggt
      721 cagcttgtcc cgctgctcct gactattcgc gccaagaata aaatccatcg ctgactgcgt
      781 atccagctca ctttcaatgg tggcggcata catcgccttc acaatggcac tctgcagctg
      841 cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc acgctgtaaa acacatttgc
      901 accgcgggtc tgcccgtcct ccacgggttc aaagacgtga atgaacgagg cacgaccgcc
      961 gggtaactca cggggtatcc atgtccattt ctgcggcatc cagccaggat agccgtcctc
     1021 gctgacgtaa tatcccagcg ccgcaccgct gtcattaagc tgcacaccgg cacggcagtt
     1081 ccggctgtcg ccggtattgt tcgggttgct gatgcgcttc gggctgacca tccggaactg
     1141 tgtccggaaa agccgcgacg aactggtatc ccaggtggcc tgaacgaaca gttcaccgtt
     1201 aaaggcgtgc atggccacac cttcccgaat catcatggta aacgtgcgtt ttcgctcaac
     1261 gtcaatgcag cagcagtcat cctcggcaaa ctctttccat gccgcttcaa cctcgcggga
     1321 aaaggcacgg gcttcttcct ccccgatgcc cagatagcgc cagcttgggc gatgactgag
     1381 ccggaaaaaa gacccgacga tatgatcctg atgcagctgg atggcgttgg cggcatagcc
     1441 gttattgcgt accagatcgt ctgcgcgggc attgccacgg gtaaagttgg gcagcagggc
     1501 tgcatccaca ctttcactcg gtgggttcca cgcccgcaac tgcccaccaa atccgctgcc
     1561 accgccgtga taaccggcat gttcacgcag
//
LOCUS       DAFAVF010000330         1557 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFAVF010000330 DAFAVF010000000
VERSION     DAFAVF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.340"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(131..1522)
                     /gene="narK"
                     /locus_tag="LGK42_004596"
     CDS             complement(131..1522)
                     /gene="narK"
                     /locus_tag="LGK42_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBK2660737.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          468 a          427 c          374 g          288 t
ORIGIN      
        1 tgggtaacat attaatggct cttaatgaaa tccggcgaaa taagccatat aatatgagta
       61 tgttgtaact aaatgacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa
      121 atattaagat ttatttttta gaatgccgac catataccgc ccaggtaatc actacgcagg
      181 cgatatagaa aatcaaaaat actttcattg cgccaactgg cgaacccgtt aatgccagcg
      241 agctaccgaa cgctttcggg ataaagaagc cacctatcgc gccaatcgca gagatgaaac
      301 ccaacgccgc cgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca gaaccccctt
      361 ctgctttcac gcgatccatt gtcagtttac gaaagatcac tgaaatcatc tggaaagtgg
      421 aaccactacc cagcccagct gtcaggaaca gcgccaggaa gaccgcgaag aacgccatga
      481 agcttccgcc ctgcccgtca gtaggtaagg tcaggaacag caggccgctg aaaatcgcca
      541 tcagaataaa gttcaccagc gtgacacgag ttccgcccag acggtcagat aatgcaccac
      601 ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa agcgtattgc agaatctgaa
      661 catccgggaa ctgcgttttt gacagcattg caaagcccgc ggagaagccg ataaaggagc
      721 cgaaggttgc cagatacagc aggctcataa tccacagatg accccgtttg agtaccggca
      781 actgctcctt gatggaggct ttcgaggtag caagatcgtt catgccaaac cacgctgcaa
      841 tggtgaagat ggcaaggaac ggcacccata tccaggacgc attcgccaga tacagctcag
      901 tcccatccgg ctgtttgacg ccctggctac caaatactgc gaaaatcgac agtgatacca
      961 ccagcggagc aaccaactgc atgacgctga cgcccatgtt tcccagacca ccattcagac
     1021 ccagcgcgcc accctgcttc tgtttcggaa agaagaagct gatgtttgcc atactggatg
     1081 cgaagttcgc gccagcaaag ccgcatagca gagagatgat gatgaagacg ctataaggcg
     1141 tggaggtatc ctgcacggca aaacccagcc agacgcaagg aataatcaga ataccggtgc
     1201 tgaacgccgt ccagcgacga ccaccgaaga taggaaccat aaaggagtat ggaacacgta
     1261 ataacgcgcc agaaaccgaa ggcagcgcag tcaacataaa tagctgatcg gtcgtaaaat
     1321 taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg
     1381 ccagcagcag acagggaacg gaaatccaca ggttacggct ggcaatacgt tgaccgcgtt
     1441 gttgccagaa cgcaggatct tccggtcgcc aatctgtaat gacagctcca gtagcccttt
     1501 cgggggcgga tgagtgactc atagacacct ctgatactcg tttcgatgtc tgccacc
//
LOCUS       DAFAVF010000331         1474 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFAVF010000331 DAFAVF010000000
VERSION     DAFAVF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.341"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            38..364
                     /gene="hdeB"
                     /locus_tag="LGK42_004597"
     CDS             38..364
                     /gene="hdeB"
                     /locus_tag="LGK42_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBK2660738.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            428..1075
                     /locus_tag="LGK42_004598"
     CDS             428..1075
                     /locus_tag="LGK42_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK2660739.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKMDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1117..>1474)
                     /locus_tag="LGK42_004599"
     CDS             complement(1117..>1474)
                     /locus_tag="LGK42_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2660740.1"
                     /translation="TQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          423 a          285 c          347 g          419 t
ORIGIN      
        1 tgttcatata taatctctat attgaatggg ttacaaaatg aatatttcat ctctccgtaa
       61 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct
      121 ggcagccaat gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
      181 agcaatgacc ccggttgcat ggtggatgct gcatgaagaa acagtatata aaggtggcga
      241 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgtaa
      301 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
      361 ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
      421 tttttatatg acagcagaat ttattatacg tcttatactc gcggcaattg cctgtggcgc
      481 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
      541 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
      601 tttagatcac gtcggactcg accccagccg tattgctgct caggtggtga cgggcgtcgg
      661 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
      721 agcggatatc tgggtgaccg ccgccatagg tatggttatt ggcagcggta tgtacgaact
      781 gggtatttat ggttcagtga tgaccttgtt ggtgctggaa gtcttccatc aattaacctt
      841 ccgcctgatg aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc
      901 aatgctcgac tggtttaagc agcaaaaaat aaaaatggat ttagtttcgt tgcaggaaaa
      961 tgaagatcat gaagtggtcg ccattgatat ccagcttcat gccacaacgt cgatagaaga
     1021 cctgttgcga ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt
     1081 gtggcagcgt agccagactc accgtaagcc tgaaattcac accagataat caatatgctg
     1141 atggcgtacc agctcattga tccgcttaag attgaggatc atcatgatat tgtgtttatg
     1201 gcagtagaac gtttttagcg atattttcag tttctccaga atatcttccg ttgtcatttg
     1261 attcgcaatg cagctcagaa cctgatgttg cttgcgcgaa agtgacacat tcgcttcagg
     1321 cacagagcag taactgccat tgatgagtgc gacaaaatca atgatagtga tagtccgcgg
     1381 aacaaaggtc accggtactt tccccagaca gcgtttaata ttaaaggggg agaaaataat
     1441 cacatggaca ggttttaatc gttcaatttg agtg
//
LOCUS       DAFAVF010000332         1463 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.342, whole genome shotgun sequence.
ACCESSION   DAFAVF010000332 DAFAVF010000000
VERSION     DAFAVF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.342"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>1463
                     /locus_tag="LGK42_004600"
     CDS             <1..>1463
                     /locus_tag="LGK42_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBK2660741.1"
                     /translation="NGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTF
                     LGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGD
                     KETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSS
                     FGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKI
                     GERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQI
                     IMGRHDDEQTLLRVDEAINKTYTRRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFR
                     VIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAV
                     HFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRIS
                     ISRWQLDLSALLASLQEEDSATTNKKTLADYARALSG"
BASE COUNT          363 a          354 c          433 g          313 t
ORIGIN      
        1 aatgggcgtg gcttctggtg cctgggcggt aaagcggcaa aaaactaccg tgaaaagtcg
       61 gtggatgtgg cgggttatga tgaacttgct gcttttgatg atgatattga acaggaaggc
      121 tctccgacgt tcctgggtga caagcgtatt gaaggctcgg tctggccaaa gtccatccgt
      181 ggctccacgc caaaagtgag aggcacctgt cagattgagc gtgcagccag tgaatccccg
      241 cattttatgc gttttcatgt tgcctgcccg cattgcgggg aggagcagta tcttaaattt
      301 ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg atgacccctc cagcgtgttt
      361 tatctctgcg agcataatgc ctgcgtcatc cgccagcagg agctggactt tactgatgcc
      421 cgttatatct gcgaaaagac cgggatctgg acccgtgatg gcattctctg gttttcgtca
      481 ttcggtgaag agattgagcc acctgacagt gtgacctttc acatctggac agcgtacagc
      541 ccgttcacca cctgggtgca gattgtcaaa gactggatga aaacgaaagg ggatacggga
      601 aaacgtaaaa ccttcgtaaa caccacgctc ggtgagacgt gggaggcgaa aattggcgaa
      661 cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt attcagcgcc cgttcctgac
      721 cgtgtggctt acctgaccgc cggtatcgac tcccagctgg atcgctacga aatgcgcgta
      781 tggggatggg ggccgggtga ggaaagctgg ctgattgatc ggcagattat tatgggccgc
      841 cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac ctatacccgc
      901 cggaatggtg cagaaatgtc gatatcccgt atctgctggg atattggcgg gattgacccg
      961 accattgtgt atgaacgctc gaaaaagcat gggctgttcc gggtgatccc cattaaaggg
     1021 gcatccgtct acggtaagcc ggtggccagc atgccacgta agcgaaacaa aaacggggtt
     1081 taccttaccg aaattggtac ggataccgcg aaagagcaga tttataaccg cttcacactg
     1141 acgccggaag gggatgaacc gcttcccggt gccgttcact tcccgaataa cccggatatt
     1201 tttgatctga ccgaagcgca gcaactgact gctgaagagc aggtcgaaaa atgggtggat
     1261 ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca atgaggcgct cgactgcttc
     1321 gtttatgcgc tggcggcgct gcgcatcagt atttcccgct ggcagctgga tctcagtgcc
     1381 ctgctggcga gcctgcagga agaggatagt gcaacaacca acaagaaaac actggcagat
     1441 tacgcccgtg ccttatccgg aga
//
LOCUS       DAFAVF010000333         1422 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFAVF010000333 DAFAVF010000000
VERSION     DAFAVF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.343"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            86..1369
                     /gene="ydfJ"
                     /locus_tag="LGK42_004601"
                     /pseudo
     CDS             86..1369
                     /gene="ydfJ"
                     /locus_tag="LGK42_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT          357 a          299 c          353 g          413 t
ORIGIN      
        1 ggatgctttt taaaaaatgc aactatctga aaaaacctag aaaacgccaa ggaaaccaca
       61 ggatgggaaa aaacacctgt gaattatgga tttccagtta tattcgctcg gcgcagcgtt
      121 agtgtttcat gaaatatttt ttcctgaatc atcaacggca atggcgttaa ttttggcaat
      181 gggaacctac ggtgcaggtt atgtggcgcg tattgtcgga gcatttattt tcggcaaaat
      241 gggcgacaga atagggcgta aaaaagtgct ctttattacc atcaccatga tggggatctg
      301 taccacctta attggtgtgt taccgaccta tgcacagatt ggtgtttttg cccccatctt
      361 gctggtgacg ctgcgtatta ttcaggggtt gggtgcaggt gcggaaattt ccggtgccgg
      421 tacgatgctg gcggaatatg caccaaaagg taagcgcgga attatctcct catttgtagc
      481 tatgggaact aactgcggaa ccttgagcgc aacggcaatc tgggccttta tgttcttcat
      541 tctcagtaaa gaggaactgc tggcgtgggg atggcgtata ccgttcctgg cgagcgttgt
      601 cgtgatggtc tttgctatct ggttacgtat gaatctgaaa gaaagcccgg tctttgagaa
      661 ggttaatgac agcaaccaac ccacagcaaa acctgcacct gctggtagca tgttccagag
      721 caaatccttc tggctggcaa cagggctgcg ttttggtcag gcgggtaact ccgggttaat
      781 tcagactttc cttgcaggct atttagtgca gacgttattg tttaacaaag caattccaac
      841 agatgcattg atgattagtt cgattctcgg ctttatgacc attccgttcc ttggttggtt
      901 atccgataaa attggtcgcc ggatcccgta tattattatg aatacctccg cgattgtgct
      961 ggcatggcca atgctttcta tcattgtaga taaaagctat gccccgagca ccattatggt
     1021 tgcactgatt gtgattcata actgtgcggt gctgggatta tttgctctgg aaaacattac
     1081 catggcagaa atgttcggct gtaaaaaccg ctttacccgg atggctattt ctaaagaaat
     1141 tggtggtctt atcgcttccg gttttggtcc tatcctggcg ggtattttct gcaccatgac
     1201 ggaatcctgg tatccgatcg ccattatgat catggcatat tcagtaattg gtttaatctc
     1261 tgcgctgaaa atgccagagg tgaaagaccg tgatttaagt gcgctggaag acgctgcgga
     1321 agatcaaccg cgtgttgtaa gagctgcgca accttccaga agtctgtaaa ccctcaatcc
     1381 cttctcttac cggagagggg atttttattc atataacaaa ac
//
LOCUS       DAFAVF010000334         1392 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFAVF010000334 DAFAVF010000000
VERSION     DAFAVF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.344"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(31..393)
                     /gene="rusA"
                     /locus_tag="LGK42_004602"
     CDS             complement(31..393)
                     /gene="rusA"
                     /locus_tag="LGK42_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBK2660742.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKS
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(390..680)
                     /locus_tag="LGK42_004603"
     CDS             complement(390..680)
                     /locus_tag="LGK42_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBK2660743.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(673..843)
                     /locus_tag="LGK42_004604"
     CDS             complement(673..843)
                     /locus_tag="LGK42_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinE from lambdoid prophage DLP12"
                     /protein_id="HBK2660744.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRSHG"
     gene            complement(843..1298)
                     /gene="ybcN"
                     /locus_tag="LGK42_004605"
     CDS             complement(843..1298)
                     /gene="ybcN"
                     /locus_tag="LGK42_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBK2660745.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1295..>1392)
                     /locus_tag="LGK42_004606"
     CDS             complement(1295..>1392)
                     /locus_tag="LGK42_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660746.1"
                     /translation="TYNPNSLLPSQMQKCTCDFLHPAPDLCGGEA"
BASE COUNT          314 a          358 c          351 g          369 t
ORIGIN      
        1 gaagaagttc tgctatatta aactcaaaca tcattcattc cccatttcgg tgatggtcag
       61 ttccagcctc ccacctttgg taacaggcat cttcacaacg cggtaatcaa cgacctgagc
      121 atcatccagc cagaaacctg ctttagtgag tgcgtcaaaa gcggcttttt gcagattatc
      181 caggtcacgg cgacggcgat ccggcatgtg gcactcaatg cggattttca caggcatagc
      241 caggccgata tccagcattg cgcttttaat gattcgggcg acgttatcgc ggtatgcctg
      301 cccctctgca ctgacgtgcg tgcgcccgcg attatggcgg taatagcgat tattgctcgg
      361 aggccagggt aatgtgatac tgtaggtatt cacgccttaa taaccccctc tttcagccag
      421 ataacctgtg ttctcgccat accttccagc gcgcattctt ttgcatatgc agcatcgaca
      481 aaatgtgtgc ggcggtcgat ttcgtcgtgg caggcagaac atgcaatggt ggcaatcagg
      541 tctggcggtt tgataccggt accgcacaat ccagccagcc ggatatgtgc cagtacagac
      601 gtttcagaat tgccattaca tacgccaggg attcttacct ggcattcccg accacgcgct
      661 gcttttctca aatcagccat gactcctcct tgctgccagt cgcaaccatt ttttatcaac
      721 caagctggcg gtatatccga gcagtgttgg tatttcggat ggcttcagct caggtttacg
      781 cttacgacga tttggtactc tgtagatgtg tccgttcatg acacgaataa gcggtgtagc
      841 cattacgcct cctgcttgtc gcgcagcagc tgaaactcgc agctctgtgg aatagtcagg
      901 tggcagccaa tattcatcgc ccaggcttca accttacaca ggaagacata catctctccg
      961 gtatcaagat cggaggtatg gcgtaacgac tggatagtag tgatttcgcc ggttacgaca
     1021 tcaaccaggt ccttggtttc ataaccgagg tatgtgtgtt tgagagcatc ttttacccat
     1081 gctgcggtag cgaacgattt ccccctgctg atgaggtatt cactgatttc gctgtaccac
     1141 atgtggctga gtgcattctg ggaaagactg cgtttctcac gccacggttt aagcaccatg
     1201 cgaaagcatt ttccgtcctc cagataaggc tggatctgct ggccgatagc ggtgaagtta
     1261 ccgcgatgca gtttgatgcc atcttgtggt aggttcacgc ttcacctccg cagaggtcag
     1321 gcgctggatg caaaaaatcg caggtgcatt tctgcatctg tgaagggaga agagagtttg
     1381 gattgtatgt gc
//
LOCUS       DAFAVF010000335         1391 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.345, whole genome shotgun sequence.
ACCESSION   DAFAVF010000335 DAFAVF010000000
VERSION     DAFAVF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.345"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            658..858
                     /locus_tag="LGK42_004607"
     CDS             658..858
                     /locus_tag="LGK42_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660747.1"
                     /translation="MDTIITWMGDRLLGGTQKSDLRQMAITGLTSAICSTILYTEKLK
                     HGEPINPNEEEKLYRLWYEGNA"
     gene            967..1068
                     /locus_tag="LGK42_004608"
     CDS             967..1068
                     /locus_tag="LGK42_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660748.1"
                     /translation="MRTYNPTSLLHSQMQKCTCDILHPAFDLCGGEA"
     gene            1065..>1391
                     /locus_tag="LGK42_004609"
     CDS             1065..>1391
                     /locus_tag="LGK42_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660749.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKELVDVVTGEITTI
                     QSLRHTS"
BASE COUNT          413 a          304 c          317 g          357 t
ORIGIN      
        1 agtcttgcaa ttggaaatga caaaaaaatt tcatatgaac ccagttggcg ctgggttttt
       61 tatttcagta gccaataatg cattcaaaat ctcttacttg agaaacggcc tatttgagat
      121 ttcagtcgtg gcaggatgat cagttgattc gagtattgac gcatttgcgt tcggagcgat
      181 tacagtagat tgtaaataat aatgagaaac acatagccac atagccaccc cgtggtattg
      241 aaaccatata atgttggatt tgaaaacaga tcttttccca tgtattaata actacatccc
      301 cgcgagtaat tcaaaaagga gggcccaatt ttgtccgagt ttttgtattc ccccgcatgc
      361 cgctgcggag cactacatct gagtgtctga ctaggggata aaattagact ggatagtgag
      421 aagaaagtgg cgcgctaggc tgtgccgagt gctaccagta caccttgggg gtgtgcagct
      481 ttcgccgaga ctgtagtggg tatcggttaa tgcacgaaaa accgagaggt cagacaacca
      541 atttgccgta ggattgtttc cggtgcgata ccggtctact aactgaaagc aatgcgaaaa
      601 agcataaact cggtccttca gtcgccctac acactattta ctaagaaggg ctgaagcatg
      661 gatacaatta ttacatggat gggagatcgt ctgttggggg ggacacaaaa atccgatctg
      721 cggcagatgg cgatcactgg attaacttcc gcgatttgtt caacaatttt gtacactgaa
      781 aaattaaaac atggtgagcc tattaaccca aacgaagaag aaaaacttta taggctttgg
      841 tatgaaggaa acgcttgaaa gaataaagca caattaaaac ctttgatttg cgataatcaa
      901 cttgccataa ttaagtaatc ggagcctgaa caactccggt gacttctgcg ctaaacgggg
      961 acgtttatgc gcacatacaa tccaacctct cttctccatt cacagatgca gaaatgcacc
     1021 tgcgatattt tgcatccagc gtttgatctc tgcggaggtg aagcgtgaac ctcccacaag
     1081 atggtatcaa attgcatcgc ggtaacttca ccgctatcgg tcggcagatc cagccttatc
     1141 tggaggacgg caaatgcttt cgtatggtgc ttaaaccgtg gcgcgagaga cgcagtcttt
     1201 cccagaatgc actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg
     1261 gtaaaacgtt cgccactcca gcttgggtaa aagatgctct caaacacact tatctcggtt
     1321 atgaaaccaa agaactggtt gatgtcgtaa ccggtgaaat caccaccatt cagtcattac
     1381 gtcatacctc c
//
LOCUS       DAFAVF010000336         1385 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.347, whole genome shotgun sequence.
ACCESSION   DAFAVF010000336 DAFAVF010000000
VERSION     DAFAVF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.347"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..1322)
                     /locus_tag="LGK42_004610"
     CDS             complement(<1..1322)
                     /locus_tag="LGK42_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBK2660750.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLV"
BASE COUNT          375 a          344 c          285 g          381 t
ORIGIN      
        1 ttgactaatg aaaatagatc attgagtgat tcgctcatcg acggatgagt aaatatctga
       61 tcgcgtaata tgctataagg cagcccggca tccatcacca ttttcactat attgatcatc
      121 tcgtgggagt caacacacag cagtgatgcc cctaatatac gttgggtttt attatcaaca
      181 atcgctttta ataccccacg agtatcattc atcactctgg cacgcggaat tgcagctaca
      241 ggcaatgtca ccacctgaat atcagcacca ctctctctgg cctgttcttc tgtcatacca
      301 accctggaca gtggcggtgt catgaatacg gaataaggca catttttccg atcatcagta
      361 ctacgtctgc cttctcccag caactcatca cgcacaatgc ggtaatcatc cagtgaaata
      421 taagtaaatt gcagcccgcc ggtaacatct cccatcgccc aaatattgtc tgcggtggta
      481 tgtaattgct tgtcaacgac aattgccccg cgctcgttta ctgcgatacc ggcattttct
      541 ggatgtaacg aagcggtagc cggttgacga ccggaagcta ttaacagtgc atccaccgcc
      601 agttgggcgt gctcgctatg cacttgcact tgattttcat ggtgactgat tcgctccaca
      661 tgggcattga ggataatatc gacgccctga tcgcgtaaaa tcgtcgcgat attatcagca
      721 atatcccgat cttcccgagg caaaaacagc gaagctgctt ctaaaatggt taccttgctg
      781 ccaaaattag cgaacataga ggcgaactca acgccaatat atccgccgcc caaaatacct
      841 aaatgcccag gcaattcttt tagattaagt aatccggtgc tgtcatatac tcctggcgtg
      901 gtggtaattc caggaattgg cggaaccacg gtttgtgcac cggtattaat aaaaattttc
      961 tcgccatgaa tttccagatt tgcctcaggc cgatgaacac gcaggctatg attattgata
     1021 aactccgcct ggccgtcgat cacgtcgata ttgggcatat ccgcaagatt atgaaaattc
     1081 ttattacgta aaaaattaac cacttcattt ttacgctgta tggcacggac aaaatctgtg
     1141 tgctgctgtg cgtcatgaac caatgttttg gttgggatgc agccgatatt aatacaggtc
     1201 ccgccataca ttgcatttga ttgttcgatg agagccactc gccaacctgc ttttgccagc
     1261 gtgacggcta atgtttttcc agccttgcca aaaccaataa taactgcctg atatttattc
     1321 atgatgattt cctgtcgaaa cgcgttcatt tgacatcatg actatagacg caaaagagca
     1381 aatac
//
LOCUS       DAFAVF010000337         1359 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFAVF010000337 DAFAVF010000000
VERSION     DAFAVF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.348"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(76..>1359)
                     /locus_tag="LGK42_004611"
     CDS             complement(76..>1359)
                     /locus_tag="LGK42_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK2660751.1"
                     /translation="VPDGDSFWEFGVNEKLLDKANFDYEKRTREVAPEIRLKTTFVFA
                     SLRTWDNPKVKLEDWLQEKRNSGKWKDIKLIDGSMLEDWLGVCPAVAAYYARYHLELM
                     PQVGVRSIKEFWDEFSTKFNPPLTEAVLLAGREKQKERFLNELRENGRKISLAADSPD
                     EVIAFAIAAIRTTEAELRHSFQSRALIIDTDDAARQLSGKRGMIFLPRDRARALAGLL
                     QQASITVVSAGADETRTDHELLIRPDSISLGKALESMGFDSDKSYQIARQCGRSLSVL
                     ARQISSSTAESPEWKDSPELLPALLAGAWSTCSEKDKLILKQLAGYTDYSQVENPLRL
                     LTKRRDSPIDRVDDIWSLRSSVDAFVHLGYLLGEEHLERFEKAVREVFSYIPEPPKAE
                     DLFVPDNGIKTSYSSWLRNGMTTVLLHMAILILPT"
BASE COUNT          388 a          318 c          262 g          391 t
ORIGIN      
        1 ggcggcacag ttggtgtggc ggcctcagtc cggaacaatt tgaaaacaag aacctcgctt
       61 aggcctgtgt ccatattacg tgggtaggat caatattgcc atatgtagca aaactgttgt
      121 cattccatta cgtaaccacg agctataaga cgttttaata ccattgtcag gtacaaacag
      181 atcttcggcc tttggtggtt caggaatata ggaaaaaacc tctcttacgg ccttctcaaa
      241 acgctccagg tgttcttcac ccagcaaata gcctaaatgt acaaaagcat ctaccgacga
      301 acgcaatgac caaatatcat ctactcggtc aattggagaa tcgcggcgct ttgttaatag
      361 ccttaatgga ttttcaactt gagaatagtc tgtatagcca gctagttgct tgagaattaa
      421 cttatctttc tctgaacatg tactccatgc accagctaat aaagcaggga gcaactcagg
      481 gctgtctttc cattcaggtg attcagccgt gctacttgat atctggcgag ccaaaacaga
      541 caaacttcgg ccacactgcc gcgctatttg ataactttta tctgaatcaa accccataga
      601 ttcaagagcc ttacctaaag atatactatc tgggcggatc agaagttcgt gatcagttcg
      661 tgtttcatcg gctccggcac ttactactgt gatagatgct tgctgtaata aaccggctaa
      721 agctcttgct cgatcacgtg gtaagaagat cattcctctt tttccagaaa gctgtcttgc
      781 agcatcatcc gtatcaataa ttagtgctcg actttgaaag gaatgtcgaa gttccgcttc
      841 agtggttctg atagctgcaa tcgcaaaagc aataacctca tccggagaat ctgcagctaa
      901 agatattttt cgaccatttt ctcgtaactc attcagaaaa cgctcttttt gcttttctct
      961 ccccgccagc aagactgctt cagttaaagg gggattaaat ttagtggaaa attcatccca
     1021 aaactcctta atgcttctaa caccaacctg tggcattagc tcaagatggt agcgagcgta
     1081 gtaagcagct acagcagggc aaacaccgag ccaatcttca agcatagaac catcaattaa
     1141 tttgatgtct ttccacttac cactattgcg tttctcttgt agccagtctt caagctttac
     1201 cttaggatta tcccatgttc ttaatgaagc gaaaacaaaa gtagtcttta gacgtatctc
     1261 tggagcaact tcgcgtgttc ttttttcgta atcaaagtta gctttatcaa gcaatttttc
     1321 attaacacca aattcccaga atgagtcgcc atcaggaac
//
LOCUS       DAFAVF010000338         1357 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.349, whole genome shotgun sequence.
ACCESSION   DAFAVF010000338 DAFAVF010000000
VERSION     DAFAVF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.349"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..208)
                     /locus_tag="LGK42_004612"
     CDS             complement(<1..208)
                     /locus_tag="LGK42_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK2660752.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMTSS
                     ESACLQAHKIQTALISQDEGCGKIK"
     gene            complement(236..>1357)
                     /locus_tag="LGK42_004613"
     CDS             complement(236..>1357)
                     /locus_tag="LGK42_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK2660753.1"
                     /translation="LNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISYEQ
                     DDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLAR
                     NPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQT
                     PLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTT
                     WGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINE
                     PIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLG
                     TGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          417 a          314 c          292 g          334 t
ORIGIN      
        1 cttttatttt gccacagcct tcatcctgac taattaatgc ggtctgaatc ttatgtgcct
       61 gaagacaggc agattcagaa ctggtcatta gctgagttgc gccctgccag tctttttgcc
      121 cggcacaacg tatggcttcc atcgcgtcag aacgtgcctg ccccgcgttg accagtaact
      181 ccataacaag ttcttcatct gcaaacatca caatctcctt cctgggtaaa tggaattatc
      241 cgttagttgt catgttattg actttgttgg cggcaataac aaaaggtaaa tagatcattg
      301 tcgccacacc taaacataaa aaaccaacca gtaaagccag ccagttcccc ccggttccga
      361 ggaaagcaat taaaggtccg ggcgtagtcc agggcacggc ataagcaacc ggtggaataa
      421 tttccatcga aacaaagaag taaccaatgg cacagttaac aatgggaacc ataataaacg
      481 gcaccatcat gatggggtta agtacaatag gaaggccaaa cattatcggt tcattgatat
      541 tgaaaatacc ggggataaat gacatttttg ccagatcacg gtattccgca cgcttagaag
      601 cgataaaaat agccaacaat aaccccaacg tcatacctga accgccgcag ttggcgaatg
      661 catcattaat agaagtccag gtaatcggat atggcgcgcc ccaggtagtg ccatgttgag
      721 cggcccagga gagattctcc agattagcct cagaaaaaac ggtttctcga attgccgatg
      781 tggtattagg tccgtggatc cccagcaccc acaggaaatt acccacagcc cccaggataa
      841 ttaccgcaaa gatattcgtt cccatatgtt tgagcggcgt ctggattaat gtgtaaataa
      901 gatcgtttag tcctgccggg gatatcagtg tcaggcaata attaagtgcg gaaaagaaca
      961 ccatgacaaa aaatattggc aataaaactt taaatgaacg cgctaccgca ggcggtacag
     1021 ctgccggcat cgttatggtg atttttgggt ttcgagctaa gcgacaaaat atttcactgg
     1081 tgatcaatgc aacgataaca gcaacaaaca acccctgcgc gccgagatat ttggtcgtca
     1141 ggaatgcttg cccatctatc atctgatatg gcgtataaac aataaaaaat gctcctattg
     1201 ccgttaatcc gcataaaaga tcatcttgct catagctaat cgccatattc ctggcgacca
     1261 ggaaagcaat cataattgac atgatattta cagaaccatt cattaccgga gtaaaaatag
     1321 cttgtgcttt ttcaaggttg gggaaaatgc tattcag
//
LOCUS       DAFAVF010000339         1350 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFAVF010000339 DAFAVF010000000
VERSION     DAFAVF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.350"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            116..>1350
                     /locus_tag="LGK42_004614"
     CDS             116..>1350
                     /locus_tag="LGK42_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBK2660754.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLI"
BASE COUNT          386 a          265 c          316 g          383 t
ORIGIN      
        1 tattatttaa ccattaaatc aacaaaaaat tttacctcat attgatttga ctgctatttt
       61 tgctattaat agccagaatt gcagtgcttt tatatctgtg tatagtgagg ttactatgtc
      121 attcggtcag ccttgtgatg agtttcccct gagttcgctg cctccactca ttcgtgatgc
      181 cgttattgaa gctcagcaaa ttacgcaggc tccccttggg cttgtggcag cgtctgccct
      241 gggcgctgtt tcacttgtct gccagaatct gattgatgtc tgcagactga atacactgcg
      301 ggggccggtc tctctttttt tcctgacgct tgccgagtcc ggagaacgaa aaaccgcagt
      361 ggacaaatta ttaatgaaac ccctgtacca gcaggagatg cagttatatt ccagatacaa
      421 aagtgaactg gctgtctgga aaaacaagga agagctttta aaggctcaga aaaaagcatt
      481 gttgtcaaaa ctgaataaag aactccgcaa aggcgcggat gagtcagaaa ccctcagaca
      541 actggaagta cttcagaaaa acagtgcgga agaacctgtg aggtataaat tcatttttaa
      601 tgatgccacg actgcggcaa ttaagaatca gctttgtggt aaatggcgtt cagttggtat
      661 tatgtcagat gaagccggta ttatatttga tggatatacc ctgtctgagt tgccttttat
      721 taacaaaatg tgggatggct ctgtattgtc tgtggacaga aagaatgaac ccgaacagat
      781 gattgaaaat gccagaatga cgttatccct catggtgcaa cccggtcttt ttgaccgata
      841 tatggagcga aaaggttctg ttgccaggga cagtggtttt cttgcccgtt gtcttatcag
      901 taaacctgca accacgcagg ggaaacgctt tattaacgga gctgtgattc cgggaggatc
      961 attaacggca tttcatgaac gattaatgga actggccaga ggcagtattg aaaaatccag
     1021 tgaggatgaa cggtattgtc tgcatttttc gcctgaagcg caaaaaatat ttattgagca
     1081 ctataatgtt ctggagcagg atttatcccc ttccggaccc ttaagcccgt ttcggggaca
     1141 tgtttcaaaa aaaacagaaa acattgcaag aattgcagcg ttatttcagt atttcagcta
     1201 tggtgaaggg aaaatctctg ccgatatcat gacgtctgct gtcgtaataa gttcgtggta
     1261 cacagatgaa tataaaaaat tatttgcgtt acctgatgaa tctgaactgc agcagaagga
     1321 cgccgaggag ctgttcgact ggctcattga
//
LOCUS       DAFAVF010000340         1316 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.351, whole genome shotgun sequence.
ACCESSION   DAFAVF010000340 DAFAVF010000000
VERSION     DAFAVF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.351"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            725..>1316
                     /locus_tag="LGK42_004615"
     CDS             725..>1316
                     /locus_tag="LGK42_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HBK2660755.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFK"
BASE COUNT          412 a          263 c          278 g          363 t
ORIGIN      
        1 ttacgcactc ccctccatta cactaataaa caccaccgac ttggtattta taaattttct
       61 aataaaagca tcattttcac tgaaacaaaa taaatatcaa tcactaagat aattaactta
      121 tctgaacaag taatcacact catatgttat tagttttctt attaatcaga aaggagggat
      181 ttataaacca gcaacttttt ttgtaggtgg agccacggtt gatttctgat atcaaccacc
      241 tgagtttatt ttcgtcgaat ggtaatgcat tgtcgcacaa acgctatctg aataattaac
      301 tgtaatggcg ttattggtat ctttgccgac attaacgcta ataaagcccc tgattgcttc
      361 ataatcgcag gatttggcca ttttgtctcc ctttagataa gagttaacaa aataaaccca
      421 ggaagaaggt accggtttcc atgttacaga cgttcccggc ataaagtgtc cgaccagggc
      481 cttattcgca gttaccagca ccctgtatgc cctgatattt cctggaacat ggatgaccgg
      541 ggcagtaatc ataccgggtt tgactgactc ccctgatatt ctgacataaa agggaataaa
      601 taaagcgaca tgcggccaca ttgtaaacaa acgttaaaca aaacagaatt tttcagttga
      661 ctactacgag taaataaaaa aatatttatc aaaaacaaac gagtggaatg gagttaactt
      721 gtctatgtac ttaaagattc ttgctactgc actctcagct cctgtagcgt ttgctgcact
      781 ggcttctgat actgggcttt catttacacc ggagaaaatc agtacggaga ttgattttgg
      841 cactttaagt ggcaaggcca aagaacgggt ttatctgcct gaagaaaaag ggcgcaaagc
      901 cagccagctg gactggaaat acagtaatgc gccaattgtc aagggggcat ttaactggga
      961 tctgttaccc agagtatctg tcggtgcctc agggtggaca acattagctg gtcgtggagg
     1021 caatatggtg gacagggact ggctggatac gagcaatccc ggaacctgga ctgatgagag
     1081 taaacatccg aatacccgtc taaattttgc taatgaattc gacctgaata ttaagggatg
     1141 gctcctgaat caaccagact atcagttagg tcttatggct ggttaccagg aaaatcgtta
     1201 tagctttaca gcaaaaggag gctcttatat ctatagtagt gagggaggct tccgggatga
     1261 aacgggatca tttccagatg gtgaaagagc tataggctac aaacaacact ttaaaa
//
LOCUS       DAFAVF010000341         1303 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.353, whole genome shotgun sequence.
ACCESSION   DAFAVF010000341 DAFAVF010000000
VERSION     DAFAVF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.353"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            203..805
                     /locus_tag="LGK42_004616"
     CDS             203..805
                     /locus_tag="LGK42_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK2660756.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(844..1269)
                     /gene="nudI"
                     /locus_tag="LGK42_004617"
     CDS             complement(844..1269)
                     /gene="nudI"
                     /locus_tag="LGK42_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBK2660757.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          361 a          285 c          268 g          389 t
ORIGIN      
        1 taaccttaag aaactaatat tagacgtaaa tattgaaatt tttatatttt ttcttattta
       61 ggctttgcat ttggcaaaat tttgaggcat tttgccgaca tcgtaggatt tttaatatta
      121 caccaactgc taattatcgc cagaaatgtc gctcaatttt acggtaattg tctggttgcg
      181 cttgtctata ggtggagttt acgtgttagc tttttgccgc tcatcattga agtcaaaaaa
      241 atatttcatc attttactgg cgctcgctgc aattgccgga ctgggtactc atgccgcctg
      301 gagtagcaat ggtttgccac gtatcgacaa taaaacattg gccagactgg cacagcagca
      361 cccggttgtc gttttgtttc gccatgctga acgttgcgat cgttcaacca atcaatgctt
      421 gtcagataaa acaggtatta cggttaaagg tacccaggat gcccgtgaac tgggcaacgc
      481 ttttagcgct gacatccctg atttcgatct ttattccagt aataccgtcc ggaccattca
      541 gtcggcgacc tggttttccg cgggtaaaaa gctgacggta gataaacgac ttcttcagtg
      601 cggtaatgag atttatagtg caattaagga cttacaaagc aaagcgcctg ataaaaatat
      661 cgttattttc acccataatc attgcctgac atatattgct aaaaataagc gtgatgcgac
      721 atttaaacct gattatctgg atggtttagt catgcatgtg gaaaaaggca aagtttatct
      781 ggatggggaa ttcgttaacc actaaatttt caatctgaca gccagtgatg gctgtcatca
      841 ttgttaaaga agacctttca aacgtaacgt ttttcgggtg gcaacattta aatcataatg
      901 caccagatct tcaggtttta cccacgcgta gtcctgaaac tcttcgttta ttttcacttc
      961 tcggttggca gaaacgcagt caaaaatcag gtaaatcata taaatctctt ccttgcgacc
     1021 atctgcatac gtcttggtgc gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa
     1081 aagcagctgt tctcccagtt cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc
     1141 gccagactcc acgccgccac ccgaaagcgc ccattgaccg gggaaaacgc cgcgatcgtc
     1201 ggccatttta cacagcaaat aagcaccatc attttgaatc aaagggcata caatagtccg
     1261 ttgtcgcata ggggtattcc ttataaataa aaagaaacca tga
//
LOCUS       DAFAVF010000342         1275 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.354, whole genome shotgun sequence.
ACCESSION   DAFAVF010000342 DAFAVF010000000
VERSION     DAFAVF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.354"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(1..898)
                     /gene="tssI"
                     /locus_tag="LGK42_004618"
                     /pseudo
     CDS             complement(1..898)
                     /gene="tssI"
                     /locus_tag="LGK42_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT          326 a          330 c          323 g          296 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcgactc attccccgcg cggtttctgc attgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg taatcctggt actggccttc ctgatcaaaa
      181 cgccctgccc agccgggtcg tttaaaggtg tagtccttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 ggattccagg ggatctcaaa ggactccggc agataacgca cggtgtcgca caggaccagg
      361 ctctggtcgg tactttttgc gcgtgctcct cataaaagaa gatgccttcc tccgccgcca
      421 tccggcacag gaaatcgtaa tcggtttcgc cgtactggac acaaaactca cgggaaggat
      481 gcggctcgct gaacagcgga ctccactcgg tcacaccgtt ttccttcagg atcgtagcga
      541 ggatgctttc gatgtcctca ttctggaaga tacggaagtt ctggcgcagc cctgtgcgcc
      601 acagcggcgg gcacaccttc atgctgtaca gcatctggtt tttgtcgttc tcccccagtt
      661 caaaccaggt caccacacct ttcacccggc gctgtacgtc atcgccctgc cataccgtca
      721 ggtaggccat tttgtccagc acctgcgcaa attcaaggga aagaaactgc tgactgacca
      781 gagaaaggtc gagggaaaaa agtgaagaga gtgactgggt gagatgaaag gaaaccaccg
      841 caaacgcgtc cggtggcagg ccgtccactt caagtgtgaa acgtaatccg gttgacatag
      901 caacctccgt gaaaatgaat aataatccgc acatattgca tgtacaacat gtcgggcgga
      961 tgagataagc gagtcgacat ctggcgttac cggggaatat cagaaaaatg tcatggttat
     1021 gtgatgattt agtctgggat tttacttcag agaaacgggt gtctgtttag tcaacaaaat
     1081 aatcacaaac gcaacggatg ggttctcgac gttattcacg acaggctcgt acattacagc
     1141 gagtaataaa aaacctgata tgcaaagtca aaccaatatt cataatgtct ttttattcac
     1201 aacaataaat aattcaataa caatcattga aatgaatgat gatttgtatt aggaatatac
     1261 gctaccgccc caccc
//
LOCUS       DAFAVF010000343         1254 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFAVF010000343 DAFAVF010000000
VERSION     DAFAVF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.355"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..258
                     /locus_tag="LGK42_004619"
     CDS             <1..258
                     /locus_tag="LGK42_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660758.1"
                     /translation="KSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTIQSLRHTSD
                     LDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQQLRDKQEA"
     gene            258..428
                     /locus_tag="LGK42_004620"
     CDS             258..428
                     /locus_tag="LGK42_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660759.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            421..711
                     /locus_tag="LGK42_004621"
     CDS             421..711
                     /locus_tag="LGK42_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBK2660760.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            708..1070
                     /gene="rusA"
                     /locus_tag="LGK42_004622"
     CDS             708..1070
                     /gene="rusA"
                     /locus_tag="LGK42_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBK2660761.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1067..1207
                     /locus_tag="LGK42_004623"
     CDS             1067..1207
                     /locus_tag="LGK42_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBK2660762.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
BASE COUNT          334 a          311 c          322 g          287 t
ORIGIN      
        1 aaatcgttcg ctaccgcagc atgggtaaaa gatgctctca aacacacata cctcggttat
       61 gaaaccaagg acctggttga tgtcgtaacc ggcgaaatca ctactatcca gtcgttacgc
      121 catacctccg atcttgatac cggagagatg tatgtcttcc tgtgtaaggt tgaagcctgg
      181 gcgatgaata ttggctgcca cctgactatt ccgcagagct gcgagttcca gcagctccgc
      241 gacaaacagg aggcgtaatg gctacaccgc ttattcgtgt catgaacgga cacatctaca
      301 gagtaccaaa tcgtcgtaag cgtaaaccgg agctgaagcc ttccgaaata ccaacactgc
      361 tcggatatac cgccagcctg gttgataaaa aatggttgcg actggcagca aggaggaatc
      421 atggctgatt tgagaaaagc agcgcgtagt cgggaatgcc aggtaagaat ccctggcgta
      481 tgtaatggca accctgaaac gtctgtactg gcacatatcc ggctgactgg attgtgcggc
      541 accggtacca aaccgccaga cctgattgcc accattgcat gttctgcctg ccacgacgaa
      601 atcgaccgcc gcacacattt tgtcgatgct gcatatgcaa aagaatgcgc gctggaaggt
      661 atggcgagaa cacaggttat ctggctgaaa gagggggtta ttaaggcgtg aatacctaca
      721 gcatcacatt accctggcct ccgagcaata atcgctatta ccgccataat cgcgggcgca
      781 cgcacgtcag cgcagagggg caggcatacc gcgataacgt cgcccgaatc attaaaaacg
      841 caatgctgga tatcggcctg gctatgcctg tgaaaatccg cattgagtgc cacatgccgg
      901 atcgccgtcg ccgtgacctg gataatctgc aaaaagccgc ttttgacgca ctcactaaag
      961 caggtttctg gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta
     1021 ccaaaggtgg gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt
     1081 taatatggca gaacttcttc gccaccgctg ggggcgtctg cgcttatatc gtttccccgg
     1141 ttctgttttg accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg
     1201 agtatgaagt cagagataac aatcaactaa tactgtttta ttgatttttg cttg
//
LOCUS       DAFAVF010000344         1249 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.356, whole genome shotgun sequence.
ACCESSION   DAFAVF010000344 DAFAVF010000000
VERSION     DAFAVF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.356"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..432)
                     /locus_tag="LGK42_004624"
     CDS             complement(<1..432)
                     /locus_tag="LGK42_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBK2660763.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSH"
     gene            complement(497..616)
                     /locus_tag="LGK42_004625"
     CDS             complement(497..616)
                     /locus_tag="LGK42_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK2660764.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(771..989)
                     /locus_tag="LGK42_004626"
     CDS             complement(771..989)
                     /locus_tag="LGK42_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK2660765.1"
                     /translation="MKKLVRTLSAVDARVVCKPNRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKTICDLKVIV"
BASE COUNT          352 a          298 c          253 g          346 t
ORIGIN      
        1 gtgtgaatta ccacccttaa ttgtcgttcc attgctgata ccacctgtat aaacgtcctg
       61 attgccactg tcgattatcg taccggtgga aatgcccccg tcatgaattg tctgtcttcc
      121 tccgttaatg gttgtattat tagcctgccc cacaaaattg ttatgacttc ctacatcttg
      181 atatccaccc gattcaataa gacttccatt agataccccg ccatgaacat tctgctggcc
      241 atggttgata atatgagtgt tatttgttgt acctcgttca tctactcttt ggctgccatc
      301 tacagtctca tcgtttacca caccaataac atcaggagtg aaggccgtca tcccaggcgg
      361 ggcatatatc aaggcagata tcaataagga aagtactgag cggcgacaat aatagggact
      421 ggtcctgttc ataaatttca tcctctgaaa agtgaatact gagtagcgtt taagcgacct
      481 tagctttgct gcaacatcag cccacaggca ccagaccagg ggattcatcc tgaagagaca
      541 gcgcaagtgt attgtgttca ccgctcatca aagacatcat gatgaaatga tgatattccg
      601 cataagaatg aggcattttt taaacgcagt gcgctgaagt gtggttggat aaaaaagtca
      661 atccattcag gaaatacggg cgtattcttt tctttcgaca atgaggccgt tggcaaaata
      721 aaatgattta cataatcgtt tctgatgaat atcttctgct cacataaaaa tcacacaata
      781 actttgagat cgcagattgt tttactttta cagcattcgt ccccccattg ttgggcaaat
      841 atagattggg ccagagcacg aaagttaata ccacgtttgc acagctcctc caacagcacg
      901 acaagatgcc acatactgcg ccccagtcgg ttcggtttac agactacccg tgcgtccacc
      961 gccgataatg tcctgaccag ttttttcagt ccggacctcc tgccacggtt ttacgttgaa
     1021 gtggtaaccc tgagcacgca gttcttcagt caggcgtggt gcaccgtaac gctgttattg
     1081 ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt tgaaccagac attagatgaa
     1141 atatttcaac caattacagc accaattcag tcactgccag cccaccaaat aaatcaaggg
     1201 gttacatgaa aacgtagccc ctttttcttt ggtagtgaca ctaaaatgg
//
LOCUS       DAFAVF010000345         1232 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFAVF010000345 DAFAVF010000000
VERSION     DAFAVF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.357"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            5..>1232
                     /locus_tag="LGK42_004627"
     CDS             5..>1232
                     /locus_tag="LGK42_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBK2660766.1"
                     /translation="MNEAAIIIVPTDISKKIREIEESYNRYINEFRIPNDHKIIVNYS
                     AGKDSTATLAVAHTLFGKQVHAVMADTDNEHNLTVEFARSIHEQIGCSPVQVVKRIYT
                     EEDFAKRRIYLQKNWSKRQMIRMGAYRGIVMPSLARSDTKFGQAWKRTAERWGIEFET
                     ALDAALSVMHPSGNSFLDCSLLHGKFPMLRDRFCTDELKIQIAFDAAIKPLLDAGDVI
                     VQWSGVRADESSKRAGYERFSRDMRDADFLYNFLPLHKWTAEDVFAVHKYFGIKPNPL
                     YMQGASRVGCMNCVLCNKEEIAETAARWPEHIKKHKQWELKVRLASRWVHWMSVGEIS
                     QRWMKQFNLPLGRNIQLFGLKPDVQHIDWSGFYGPRGDLNSPGVYEVVEWAKTGRGGK
                     VYDLVKASLDIEVCSSR"
BASE COUNT          345 a          277 c          307 g          303 t
ORIGIN      
        1 cggtatgaac gaggcagcaa taattattgt cccaacagac atcagcaaga aaattcgcga
       61 aatagaagag tcctacaaca gatatataaa tgaatttcgc atccctaatg atcataagat
      121 aatcgtgaat tattcagcag gaaaagacag cactgccaca ctcgcagtgg cgcacacatt
      181 attcggcaaa caggtacatg ctgttatggc agacacggat aatgagcata atctaactgt
      241 cgaattcgcc aggagcattc atgagcagat cggttgtagc cctgtgcagg ttgtgaagcg
      301 gatttacact gaagaagatt ttgccaaacg acgaatttat ctgcaaaaaa actggtctaa
      361 acgacagatg atccgcatgg gagcataccg tggcatagtc atgccttccc tcgcacgttc
      421 tgacaccaaa ttcgggcaag cctggaagcg cacagcagaa cgctggggaa ttgagttcga
      481 aactgctctt gatgctgcgc tttccgttat gcacccatcc ggtaatagct ttttggattg
      541 ctcccttctg catggcaagt tccctatgct gcgcgaccga ttctgtacag acgaactgaa
      601 aattcagata gctttcgacg cagccataaa gccgctcctt gatgctggcg atgttatcgt
      661 acaatggtct ggtgttcgcg cggatgagtc ctctaagcgc gctggatatg agaggttttc
      721 ccgcgatatg cgcgacgccg attttctcta caacttcctg ccgctccaca aatggacggc
      781 tgaggacgta ttcgcagttc acaaatattt cggaattaag ccaaatccac tttatatgca
      841 aggtgcgtct cgtgttggct gcatgaattg cgtgctatgc aacaaagagg aaattgcaga
      901 gacggctgcg cgctggccag agcacatcaa aaagcacaaa caatgggagt tgaaggtacg
      961 ccttgctagt agatgggtac actggatgag cgtaggagag ataagccaac gctggatgaa
     1021 acagttcaat ctgccacttg gtcgaaatat tcaacttttt gggctaaaac ctgatgtgca
     1081 acacatcgac tggtcaggat tttatggtcc tcgtggagat cttaattctc cgggtgttta
     1141 tgaagtcgta gagtgggcaa aaacagggcg cggtggcaag gtctacgatc ttgtaaaagc
     1201 aagtctcgat atagaggttt gctcttcacg ct
//
LOCUS       DAFAVF010000346         1226 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.358, whole genome shotgun sequence.
ACCESSION   DAFAVF010000346 DAFAVF010000000
VERSION     DAFAVF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.358"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="LGK42_004628"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="LGK42_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBK2660767.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFTQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGR"
BASE COUNT          315 a          309 c          309 g          293 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgaacggctt gttccgcgcg ccacttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg taatcctggt actggccttc ctgatcaaaa
      181 cgccctggcc agccgggtcg tttaaaggtg tagtccttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca ggaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
      661 tcaaaccagg tcaccacgcc tttcacccgg cgctgtactt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgta aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcat ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctcca gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct
     1081 caacctcttg tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa
     1141 acgcaaagtt aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt
     1201 aacattatta aaatgaatga ttgcat
//
LOCUS       DAFAVF010000347         1216 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.361, whole genome shotgun sequence.
ACCESSION   DAFAVF010000347 DAFAVF010000000
VERSION     DAFAVF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.361"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..532
                     /locus_tag="LGK42_004629"
     CDS             <1..532
                     /locus_tag="LGK42_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660768.1"
                     /translation="TYTANKPTGLTDGTVYVIYSSHIKPTAADVGALSLSGGQLNGAL
                     GIGTSSALGGNSIVLGDNDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGR
                     VNPSDYGNFDSRYVRDVRLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFR
                     PIQKYINGTWYNVAQV"
     gene            536..1063
                     /locus_tag="LGK42_004630"
     CDS             536..1063
                     /locus_tag="LGK42_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2660769.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVI
                     KRIYTADEQQQQAESKKAALLSEAESVIQPLERAVRLNMATDEERARLELWERYSVLV
                     SRVDPANPEWPEIPQ"
BASE COUNT          353 a          231 c          310 g          322 t
ORIGIN      
        1 aacttataca gcgaataaac caacaggcct gacagatgga actgtatatg taatttacag
       61 ttcgcacatt aaaccgactg ctgctgatgt tggggcgttg tcattatctg gaggtcaatt
      121 gaatggtgca ctgggcatcg gtacatccag tgctcttggc ggtaactcga ttgttttggg
      181 tgataatgac acgggcttta aacaaaatgg cgacggtaat ctggatgttt atgctaataa
      241 cgtccatgtt atgcgctttg tctccggtag cattcaaagt aataaaacca taaatattac
      301 ggggcgtgtt aatccctcgg attacggtaa ctttgattcc cgctatgtga gagatgtcag
      361 acttggcaca cgtgttgtcc agaccatgca gaaaggggtg atgtatgaga aagcagggca
      421 tgtaattaca gggcttggta ttgtcggtga agtcgatggt gatgaccccg cagtattcag
      481 accaatacaa aaatacatca atggcacatg gtataacgtc gcacaggtgt agtttatgca
      541 gcatttaaaa aatattactg cgggtaatcc aaaaactgtt gcccagtatc aactgacaaa
      601 aaattttgat gttatctggt tatggtccga agagggaaaa aactggtatg aggaagtaag
      661 taattttcag gaagacacga taaagattgt ttacgacgag aataatataa ttgtcggcat
      721 cacccgagat gcttcaacgc ttaaccctga aggttttagc gttgtcgagg ttcctgatat
      781 tactgccaac cgacgtgctg atgactcagg taaatggatg tttaaggatg gtgccgtgat
      841 taagcggatt tatacggcag acgaacagca acaacaggcc gaatcaaaaa aggccgcgtt
      901 actttccgaa gcggaaagcg ttatccagcc gctggaacgc gccgtcaggc tgaatatggc
      961 gacggatgag gagcgcgcac gactggagtt atgggaacgc tacagcgttc tggtcagccg
     1021 tgtggatcct gcaaatcctg aatggccgga aataccgcaa taagttgtat gatatctgga
     1081 gtgagctaac gtatctatgg cacagagtaa aacttaatct gacagtctgc tctgtaccaa
     1141 aagcggaact tgcttacatc gtgctatgtt agtttgctgg gagcaggtca ttatctacgt
     1201 gattgctatt ttataa
//
LOCUS       DAFAVF010000348         1216 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFAVF010000348 DAFAVF010000000
VERSION     DAFAVF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.362"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            48..941
                     /gene="galF"
                     /locus_tag="LGK42_004631"
     CDS             48..941
                     /gene="galF"
                     /locus_tag="LGK42_004631"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBK2660770.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT          330 a          263 c          318 g          305 t
ORIGIN      
        1 tatggcaata atttacgcac tatcagccac ttagaggtag gaaaagaatg acgaatttaa
       61 aagcagttat accggtagca ggtcttggga tgcatatgtt gcctgccact aaggcgattc
      121 ccaaagagat gctaccgatc gtcgacaagc caatgattca gtacattgtt gacgagattg
      181 tggctgcagg gatcaaagaa atcctcctgg taactcatgc gtccaagaac gcggtcgaaa
      241 accacttcga cacctcttat gaattagaat ctctccttga acagcgcgtg aagcgtcaac
      301 tactggcgga agtgcaatct atctgcccgc cgggcgtgac tattatgaac gtgcgtcagg
      361 gcgaaccttt aggtttaggt cactccattt tgtgtgcacg acccgccatt ggtgacaatc
      421 catttgtcgt ggtgctgcca gacgttgtga tcgacgacgc cagcgccgac ccgctacgct
      481 acaaccttgc tgccatgatt gcgcgcttca atgaaacggg ccgtagccag gtgctggcaa
      541 aacgtatgcc gggtgacctc tctgaatact ccgtcatcca gaccaaagaa ccgctggacc
      601 gtgaaggcaa agtcagccgc attgttgaat ttatcgaaaa accggatcag ccgcagacgt
      661 tggactcaga catcatggcc gtaggtcgtt atgtgctttc tgctgatatt tggccggaac
      721 ttgaacgcac gcagccaggg gcatggggac gtattcagct gacagatgcc atcgctgaat
      781 tggcgaaaaa acaatctgtt gatgcaatgc tgatgactgg tgacagctac gactgcggta
      841 aaaaaatggg ctatatgcag gcgtttgtga agtatgggct acgtaacctg aaagaagggg
      901 cgaagttccg caaaggtatt gagaagctgt taagcgaata atgaaaatct gaccgaatgt
      961 tacggttgat aagaaaatta taacggcagt gaagattcgt ggcgagagta atttgttgcg
     1021 aatattcctg ccgttatttt atataaacaa tcagaataac aacgagttag taataggatt
     1081 ttcgtcaaag ttttccagga ttttccttgt ttccagagcg gattggtaag acaattagcg
     1141 tttgaatttt tcgggtttag cgcgagtggg taatgctcgt cacatcgtag gcatgcatgc
     1201 agtgctctgg tagctg
//
LOCUS       DAFAVF010000349         1200 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.364, whole genome shotgun sequence.
ACCESSION   DAFAVF010000349 DAFAVF010000000
VERSION     DAFAVF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.364"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..857
                     /locus_tag="LGK42_004632"
     CDS             <1..857
                     /locus_tag="LGK42_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBK2660771.1"
                     /translation="SSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLY
                     FNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCH
                     IFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDT
                     WGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENV
                     ERLTGDNPEYAIPQKVTISIGAVVTQENTLNPNEIYRLADNALYEAKETGRNKVVVRD
                     VVNFCESP"
     gene            complement(898..>1200)
                     /locus_tag="LGK42_004633"
     CDS             complement(898..>1200)
                     /locus_tag="LGK42_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK2660772.1"
                     /translation="NVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVES
                     FFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          338 a          246 c          271 g          345 t
ORIGIN      
        1 tttctagtca ttatgagagc tacaatttac acattgccga gttaaccaat gaaaatggtc
       61 aggtggtttg gaaagcctca tatgttacca taatgatttt catgtggtta accttgcttt
      121 cggttaatct atactttaat ggtttacgct atgatatctg gaatggagtg acggtgattg
      181 ctttttgtgc cgtactgtac aatatctctc tgttatttat gagtaggtat agcgtgtcca
      241 cttggtatat tagtcgcact attgaagtag tcagtaagtt aactgttatg gtaatattta
      301 tgtgccatat attcagtgcg ctacgagtaa caaagaacat tgcacatcgc gatcccttaa
      361 ccaatatatt taacagaaat tattttttta atgaactgac agttcaatca gcatcagcca
      421 aaaaaacgcc ttattgcgtc atgattatgg atatcgacca cttcaaaaaa gtcaacgaca
      481 cctgggggca tccggttggc gatcaggtga taaaaacagt ggtgaatatc attgggaaaa
      541 gcatacgacc agatgacctt ttagcgcgcg tcggcggcga agagtttggc gtcttgctga
      601 cggacatcga tactgaacgt gcgaaagctt tagcggaaag gattcgggaa aatgttgagc
      661 gtttaactgg cgataatcct gaatacgcta taccacaaaa agtgacgatt agtattggcg
      721 ctgttgtgac tcaggaaaat acgttaaacc caaatgagat ctatcgactg gctgataacg
      781 cactttacga ggcgaaagag accgggcgta ataaggtggt tgtgagggat gtggtgaatt
      841 tttgtgagtc accataaagc ggcattttga tcctacccac gtaatatgga cacaggccta
      901 agcgaggttc ttgttttcaa attgttccgg actgaggccg ccacaccaac tgtgccgccg
      961 ccaccgattg taatcacatt cgatataatt aaacaccgtt gcccgcatta tttcccggct
     1021 gataaagtgt tctccatgga tacattccac tttcagcgaa tgaaagaagc tttccacgca
     1081 ggcattatcg tagcagcaac cttttgcgct catacttcca cgcagattat gccgcttcag
     1141 ttgcgcctga taatctgctg aacagtactg gcctccacgg tccgtgtgaa cgataacgtt
//
LOCUS       DAFAVF010000350         1185 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFAVF010000350 DAFAVF010000000
VERSION     DAFAVF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.365"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            370..939
                     /locus_tag="LGK42_004634"
     CDS             370..939
                     /locus_tag="LGK42_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBK2660773.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
BASE COUNT          276 a          324 c          314 g          271 t
ORIGIN      
        1 gacgctgaca gtagtcagca ggaccacaat aatcagtgcc agcagtatgg taacgatacg
       61 gtctgtattc cggtaatagc cgaaggggtc gacagaacca caatacgggc aacatgtatt
      121 atgagtaatc gcgtttccac agtccctgca cttctttttg cgtatcacca taactccctc
      181 ctcacagcct tacccgtaac cggtttttta cattaaaaaa acaacgctga aaattcaacc
      241 gtcagtccgg agacgatagt tggggtatca cagagtgcct gagacagtgt cctgccggag
      301 gtcacttcag ggactctgcg cattttttac gacgtggcta ttccgtatga accatacgga
      361 gatttaacca tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacggtg
      421 cggcacgcac tgaatgttca tccccgcctg acggcattgc gggttgacct gcgtttcccg
      481 gatgtaccgg cagcaacgga cgcagctgtg atatcccgct tcatcaatgc cctgaaagcc
      541 cgaatcgacg cttaccagaa acgtaagcat cgggaaggta aacgcgtgca tcccaccacc
      601 ctgcattacg tctgggcccg ggagtttggg gagtgcaaag gtaaaaaaca ctatcacctg
      661 atgctgctgg ttaaccggga tacctggtgt cgtgccggtg attaccgtgc tccgggatca
      721 ctggccggga tgattaaaca ggcgtggtgc agtgccctgg gagtggatgt cgggtgccat
      781 gccacgctgg tgcattttcc ggcctggccg gcggtgtggc tggaacgcga tgatgacact
      841 ggctttcagc aggtgctgga acgtgctgac tatctggcga aggaacatac caaagctcac
      901 tgcaccggtg agcgcaattt tggctgtagc cggagttgag cacgactggc gcgccaatat
      961 ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg atgtatccac tcgcccactg
     1021 actgtttttt acgtcagtgt tccattgtgt gacaaccaca gtgctgtaac agcaaccagc
     1081 ctgctttacc caaactccag tccagatttt aactgaaccg ccattcacac cctgaccacg
     1141 ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg cggct
//
LOCUS       DAFAVF010000351         1184 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.366, whole genome shotgun sequence.
ACCESSION   DAFAVF010000351 DAFAVF010000000
VERSION     DAFAVF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.366"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..299)
                     /locus_tag="LGK42_004635"
     CDS             complement(<1..299)
                     /locus_tag="LGK42_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBK2660774.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQEL"
     gene            688..921
                     /locus_tag="LGK42_004636"
     CDS             688..921
                     /locus_tag="LGK42_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBK2660775.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            978..>1184
                     /locus_tag="LGK42_004637"
     CDS             978..>1184
                     /locus_tag="LGK42_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBK2660776.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSK"
BASE COUNT          369 a          283 c          238 g          294 t
ORIGIN      
        1 ttcagttcct gtgcgtcggc ctgcgcacgc gtaagtcgat ggcgttcgta ctcaatagtc
       61 cctggctgga gatctgcctc gctggcctgc cgcagttctt caacctcccg gcgcagcttt
      121 tcgttctcaa tttcagcatc cctttcggca taccatttta tgacggcggc agagtcataa
      181 agcacctcat tacccttgcc accgcctcgc agaacgggca ttccctgttc ctgccagttc
      241 tgaatggtac ggatactcgc accgaaaatg tcagccagct gctttttgtt gacttccatt
      301 gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag cccgttttca
      361 gcacctgtcg tttcctttct tttcaggggg tgttttaaat aaaaacatta ggttacggcg
      421 aagaagaacg gaaacgcctt aaaccggaaa attgtcataa atagcgaaaa cccgcgaggt
      481 cgccgccccg taacctgtcg gatcaccgga aaggacccgt aaagtgataa tgattatcat
      541 ctacatatca caacgtgcgt ggaggccatc aaaccacgtc aaataatcaa ttatgacgca
      601 ggtatcgtat taattgatct gcatcaactt aacgtaaaaa caacttcaga caatacaaat
      661 cagcgacact gaatacgggg caacctcatg tcaacgaaga acagaacccg cagaacaaca
      721 acccgcaaca tccgctttcc taaccaaatg attgaacaaa ttaacatcgc tcttgagcaa
      781 aaagggtccg ggaatttctc agcctgggtc attgaagcct gccgccggag actgtgctca
      841 gaaaaaagag tttcttctga agcaaacaaa gaaaagagtg acattactga attgctcaga
      901 aaacaggtca gaccagattg aagcaattta gataatcgtg cagactacgc ccctcatatc
      961 acatggaagg tactacaatg gctcaggttg ccatttttaa acaaatattc gataaagtgc
     1021 gaaataattt aaactatcac tggttttatt ctgaactaaa acgtcacaat gtctcacatt
     1081 acatttacta tttagctaca gagaatattc atcttgttct tgaaaacgat aatacggttt
     1141 taataaaagg acagggtaag gttgtaaatg taagattttc aaaa
//
LOCUS       DAFAVF010000352         1181 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.367, whole genome shotgun sequence.
ACCESSION   DAFAVF010000352 DAFAVF010000000
VERSION     DAFAVF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.367"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            196..272
                     /locus_tag="LGK42_004638"
     tRNA            196..272
                     /locus_tag="LGK42_004638"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Arg,seq:tct)
     gene            complement(288..>1181)
                     /locus_tag="LGK42_004639"
     CDS             complement(288..>1181)
                     /locus_tag="LGK42_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2660777.1"
                     /translation="EGIRLKDISEAKIYSAVSRMHNRKTKEIWKQKVQAAIRKGKELP
                     VYEPKPVSTQTKAKHLAMIKAILRAAERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAK
                     RLIDECPEPLKSVVKFALATGLRKSNIINLEWQQIDMQRRVAWVNPEESKSNRAIGVA
                     LNDTACKVLRDQIGKHHKWVFVHTKAAKRADGTSTPAVRKMRIDSKTSWLSACRRAGI
                     EDFRFHDLRHTWASWLIQSGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARKIDD
                     IFGDNVPNMSHSGITEDIKKA"
BASE COUNT          260 a          304 c          258 g          359 t
ORIGIN      
        1 ttgtactttg atacatgaaa atacgggttt tcttgattca gacgcgcagc ggtgtgtgtt
       61 tgtttgccgc tatagcgaaa taaatcagaa aatcagacgc ggtcgttcac ttgttcagca
      121 accagatcaa aagccattga ctcagcaagg gttgaccgta taattcacgc gattacaccg
      181 cattgcggta tcaacgcgcc cttagctcag ttggatagag caacgacctt ctaagtcgtg
      241 ggccgcaggt tcgaatcctg cagggcgcgc cattacaatt caattagtta cgccttcttt
      301 atatcctccg taattccaga gtgggacata tttgggacat tatcaccaaa aatgtcgtct
      361 attttcctcg catgctctgt caaatgatta ggcgcaaggt gagcatacct acgaaccatt
      421 tctatggact cccatccgcc catttcctga agcactgata atgggacgcc tgactgaatc
      481 agccagcttg cccaggtgtg tctgaggtca tggaaacgga aatcttcaat tcctgcacga
      541 cgacaagctg atagccatga tgtcttgctg tcgatgcgca tcttcctgac cgcaggcgtt
      601 gatgttccat ctgctcgctt agccgccttg gtatgtacaa acacccattt gtgatgcttg
      661 cctatttgat cacgcaacac tttacaggcg gtatcgttca gcgccacacc aatggcgcgg
      721 tttgatttgc tctcttctgg attcacccag gcaactcgtc gctgcatgtc gatttgttgc
      781 cattccagat ttatgatgtt cgactttctc agaccagttg ccagcgcaaa cttgacgaca
      841 gatttcagtg gttcggggca ctcatcaata aggcgttttg cttcctcctt ttccagccat
      901 ctgactcgct tgtttctgac cgctggtatc ttgatgacag gcgctttttc cagccacttc
      961 cagtcgcgtt ctgcagcacg gagaatggcc tttatcatgg caagatgctt tgcctttgtc
     1021 tgagttgata ctggctttgg ttcataaaca ggcagttctt tacctttcct gatggcggcc
     1081 tgaactttct gtttccatat ttctttcgtc tttctgttat gcattctgct tacagcagag
     1141 taaatctttg cctccgagat atctttaagc cttataccct c
//
LOCUS       DAFAVF010000353         1179 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.368, whole genome shotgun sequence.
ACCESSION   DAFAVF010000353 DAFAVF010000000
VERSION     DAFAVF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.368"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(24..995)
                     /gene="manX"
                     /locus_tag="LGK42_004640"
     CDS             complement(24..995)
                     /gene="manX"
                     /locus_tag="LGK42_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK2660778.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT          278 a          301 c          263 g          337 t
ORIGIN      
        1 tgagtgataa tcaacacaat acgttactta tcgattttgc tgatcagatc catcattttc
       61 agtttcggat cggtggaaac cttacggact tccagctcaa taccgcgcgc attcagtttc
      121 ttgaacgcct cgatatcttt ttcatcaacc gaaaccgcgt tattcacctg ggttttaccc
      181 tgacggaatg ccataccacc gacgttaaca gaggtgattt tcacgccgcc ttcaacgaga
      241 cgctctacat ctgttgggtt ggtaaataac agcattacgc gttcgccagc atatttcggg
      301 ttgttgtaga cgcgaatcat tttggcaaca tcaactacgt gtgctgttac gcccggaggt
      361 gcaacctggg tgagcagtgt cttacgaacg gtatccgcag ccacttcatc actaacaaca
      421 ataatacggg agacattggt ttctttggtc cagcgggtgg cgacctgacc gtgaatcaga
      481 cggtcgtcga tacgcgcaag gccaataacc atgtagtcgt ttggccccat tggttttgcc
      541 ggagttggag ccgcttttgg tgctgcggca gcgggtgctg gcgcggcttt ttcaaccggt
      601 ttggctttca gagctttcac gccttcacgg cctgtttcta ctgccagtgc caccagttca
      661 tcaaagcttg ggtcatcatc acgggccatt aacgtttcca cgagcattgg aatgttaacg
      721 cctgcaatga cttcataatg ctctttgtcg acgacaatgc ggctggcagc attgaacggg
      781 ctgcctcccc atgtatcaac gagaaacagc acgcctttag tggtgtcgag ttttgccaac
      841 tgagcgttgt acttttcaat cagcgtttcg gcattttcac ctggaacgaa atcgatccag
      901 ccgacgtttt cctgctcgcc taacagcatt tctgccgttt taagcaactg ctctgcagcc
      961 caaccatgtg tgcctataac aatagcaatg gtcacttgct acctccttta ttatcgttaa
     1021 cacctcaacg tgccagatgt atttttgaat cgtcgtccac aatcgaatcg attcagataa
     1081 gggctacaac caaaaacccc tgcgtttcgc gatttatttt agatatcgaa aaaattattt
     1141 tatgtgatga agatccgtaa tttaactttc gattagcag
//
LOCUS       DAFAVF010000354         1178 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFAVF010000354 DAFAVF010000000
VERSION     DAFAVF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.369"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..379)
                     /locus_tag="LGK42_004641"
     CDS             complement(<1..379)
                     /locus_tag="LGK42_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660779.1"
                     /translation="MKDVSANFFDDFYLQVCTACEDKLLELEWKLEVEKKESNQDVLR
                     TYRQRAYDKIEEFKIIEYSKIQCQYFVDMTNSDNIRYSQEFINVDGIMVEKPFIYKET
                     YAEYRIEIRGKMDGYKLTGLARLY"
     gene            493..732
                     /locus_tag="LGK42_004642"
     CDS             493..732
                     /locus_tag="LGK42_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBK2660780.1"
                     /translation="MLQNPPSLHTSSYANIPDSPRILRLKDVMKKMGISRSTIYDWLN
                     PKSPRYDGTFPKQKRLGRQSVGWLESELNEWVMRR"
     gene            898..>1178
                     /locus_tag="LGK42_004643"
     CDS             898..>1178
                     /locus_tag="LGK42_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBK2660781.1"
                     /translation="MSDSFERGINVLKQPLSILPEHISDLVLEHIRQSIHYEPVIGIM
                     GKTGAGKSSLCNALFEHPLSPVSHAEGCTRKPLHFTLNAGGRSLTLVDLP"
BASE COUNT          342 a          282 c          208 g          346 t
ORIGIN      
        1 catatagtct tgctaatcct gtcagtttat acccgtccat ttttcctcga atttcaatcc
       61 gatattctgc atatgtttcc ttataaataa aaggtttctc aaccattatt ccatcaacat
      121 ttataaactc ttgtgagtag cgtatattgt cgctattagt catatctaca aaatattgac
      181 attgtatttt tgaatactca ataattttaa attcttcaat tttatcatag gctctttgcc
      241 tgtaagtgcg cagaacatcc tggttgctct cctttttttc gacttcaagt ttccattcca
      301 gctcaagaag tttgtcctca caagcggtac aaacttgcag atagaaatca tcaaaaaaat
      361 tcgcactcac atccttcatg aaaagtaaat ccctaaagtg attttatttt aatgttactt
      421 ccagtgtatg acagccccag cgtggttggc aatatggacc cacctgaaca aactcatact
      481 aaggaagcct agatgttaca gaaccctccc tccttacata catccagtta cgcaaatatt
      541 cctgattcac cgaggatctt gaggctaaaa gatgtcatga aaaaaatggg aatttcacgc
      601 tcaaccatat acgactggct taatccaaaa tcgccacgct acgatggaac atttccaaaa
      661 caaaaacgct tgggaaggca atccgttggc tggctggagt cagaactgaa tgaatgggta
      721 atgcgacgtt agatatcatt tcaccgtatc agtgaaccgg ggcaatgccc ccggtttgct
      781 ccacaaaatc acattcctcc ccggaattcg aatgcaaaaa aaaacagacc tgtattaccg
      841 caatgaatac attgcagcga agttactgta tcgaattttt ttcattccgg aggctacatg
      901 tctgattcct ttgaacgggg cattaatgtc ctgaagcaac ctttatccat tcttcctgaa
      961 catatctccg atttggttct ggagcatata cgccagagta ttcattacga acccgtgatt
     1021 ggcatcatgg gcaaaacagg tgccggaaaa tcctcgcttt gcaatgccct ttttgaacat
     1081 cctctcagcc cagtcagcca tgcagaaggc tgcacacgta aacctttgca ttttaccctg
     1141 aacgctggag gacgcagcct gacgctggtc gatctgcc
//
LOCUS       DAFAVF010000355         1159 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFAVF010000355 DAFAVF010000000
VERSION     DAFAVF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.371"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>1159
                     /locus_tag="LGK42_004644"
     CDS             <1..>1159
                     /locus_tag="LGK42_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBK2660782.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP"
BASE COUNT          222 a          410 c          347 g          180 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
      961 ctatgtgctc gacggcctgc tgtaccacga gtccgacctg cggatcgagg agcactacac
     1021 cgacacggcc ggcttcaccg atcacgtctt tgccctgatg cacctgctag gcttccgctt
     1081 cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac gtgccgcagg gcgtgcaagc
     1141 ctacccgacg ttgcgcccg
//
LOCUS       DAFAVF010000356         1158 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.373, whole genome shotgun sequence.
ACCESSION   DAFAVF010000356 DAFAVF010000000
VERSION     DAFAVF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.373"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(1..274)
                     /locus_tag="LGK42_004645"
                     /pseudo
     CDS             complement(1..274)
                     /locus_tag="LGK42_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            342..1158
                     /locus_tag="LGK42_004646"
                     /pseudo
     CDS             342..1158
                     /locus_tag="LGK42_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          298 a          302 c          303 g          255 t
ORIGIN      
        1 caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg
       61 gggttggcat attcagaaat gtatcgagag attcagtcca tttcaccgta tgggttaagt
      121 gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct gttacgttca
      181 tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg cgcattgcct
      241 gaacgccgtc ttccatcaaa cgcgtgtgcc aggcactgat cttacccagc aatagtggac
      301 acgcggctaa gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa
      361 ccagtaaaaa accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg
      421 ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct
      481 acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt
      541 ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc
      601 tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      661 acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      721 ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca
      781 ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg
      841 cctgacggat gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag
      901 cctgcgccgt cagggactga gggcaaaggc ctcccggaag ttcagcccgg tcagctaccg
      961 cgcacacggc ctgcctgtgt cagaaaatct gttggagcag gatttttacg ccagtggccc
     1021 gaaccagaag tgggcaggag acatcacgta cttacgtaca gatgaaggct ggctgtatct
     1081 ggcagtggtc attgacctgt ggtcacgtgc cgttattggc tggtcaatgt cgccacgcat
     1141 gacggcgcaa ctggcctg
//
LOCUS       DAFAVF010000357         1139 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.374, whole genome shotgun sequence.
ACCESSION   DAFAVF010000357 DAFAVF010000000
VERSION     DAFAVF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.374"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..395
                     /locus_tag="LGK42_004647"
     CDS             <1..395
                     /locus_tag="LGK42_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660783.1"
                     /translation="FTGVKPLYSIIKCSTEIFYDWRMLILCFGFMSFSFLNVHVILLT
                     IIKSFLIKKTKVVNFATDITIQLTLIFLLIAIVIAPLIAPFVTGYVNANYHPCGNNTG
                     IFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(532..>1139)
                     /locus_tag="LGK42_004648"
     CDS             complement(532..>1139)
                     /locus_tag="LGK42_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660784.1"
                     /translation="VSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMRKY
                     YTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIVYQ
                     DTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYEYK
                     VLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          319 a          231 c          208 g          381 t
ORIGIN      
        1 tttttaccgg agttaaacct ctatattcaa taataaaatg ctccacggaa atattttatg
       61 attggagaat gttgattctg tgctttggtt tcatgtcgtt ttccttttta aatgttcatg
      121 taattttact tacgataata aaatcattct taatcaaaaa aacgaaggta gtcaatttcg
      181 ccacagacat aacaatacag ctaactctta tatttttact aatagctatc gtaatagcac
      241 ctttaatagc tccttttgtc acaggatatg tcaatgctaa ttatcacccc tgcggtaata
      301 atacggggat tttcccagga gcaatctata ttaaaaatgg catgaaatgt aataatgaat
      361 atatttctcg caaggaagat tcagcagtta aataattaaa tattctaaaa caaacagttt
      421 ttaactccgg attccatgtc ctttcttgca ctgtctttgt caagaaaaat ttcttatgca
      481 aagaattttg tattattgcc gccagaaatt cctggcggca gatataattt attattgata
      541 gcggttaacc aaatctttta ccatcgcttt ataggtcttt tgcttaagtt gcatgatttg
      601 ctgctctgtc atatcagtcg tactggcttc attcttgtcg ggcaatggtg gaagcacttt
      661 atattcataa ttcaccggcg accaggtagc ccactcacct gttgccacat ccaccagcgt
      721 gaaacgtacc aggtagcgca gggagatagc gtcggtgagt ttttcatttt tatagtcgga
      781 ccatactgtt gatttcagcg cagagtcata ttttcctgtc tgcaatgtat cctggtaaac
      841 aataatcgct ttctgatgtc cttttgcagc cacaaaacgc agtgcctgca tccagttcat
      901 attctcagca ctggcaacat cctcgttctc atttttatct ttgtttttgt tacaagtcag
      961 aggcttttgc ctgtccggga taccagagaa tgtggaaaca gtataatatt ttcgcatctc
     1021 ctcctgcata atggtttccg gtgcgcggtt gccggattga acaagtatta caggagaatt
     1081 aaggggaacg cggaactcat ctccatcaag cgcagcttga atatcctctt cggatacgg
//
LOCUS       DAFAVF010000358         1128 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.375, whole genome shotgun sequence.
ACCESSION   DAFAVF010000358 DAFAVF010000000
VERSION     DAFAVF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.375"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            359..>1128
                     /locus_tag="LGK42_004649"
     CDS             359..>1128
                     /locus_tag="LGK42_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660785.1"
                     /translation="MRIMSNAKGFPSIASGESSLTGFISQVDGTPAYTGVFQGWATRS
                     LYTYRWNPTIGPQWTRHARKDEVDRLTQLTGNTRVYCQNARKFFSIYDSGTWGYYDAE
                     NSEWIPLSIAAGGTGAATIDGARTNLGLGRNNSPQLNSLFLDRYSDSTNTYTSSGILH
                     TRLLATDSTVRLGADMYVETLSNEPGQLTIRFTYDGSTGASKYLNLNSEGNLIVDSAI
                     LRSTVEKPLQIRSANPAIRFNETDRPANTPTYTLIANAG"
BASE COUNT          354 a          248 c          267 g          259 t
ORIGIN      
        1 ctcggcaacg gcggcaggaa attccgcgaa ggccgcaaaa acgtctgaga cgaatgcgga
       61 taacagcgca caggcggcag cggcctcaca aactgcatcg gcaaactccg cgacagcagc
      121 caaaaaatca gaaaccaacg cgaaaaatag cgaggcagca gcaaagagca gcgaaacaaa
      181 cgctaaagcc agcgaaacta atgctaaatc cagtgaaaca aacgcggcga aatctgcggc
      241 ggacgcactt aattatcgca accaggcgca agtaattgtt ggcgataata tcggtcttgg
      301 ctcagccccg cgtgattgtc ctgatatttc cggcaaccca tcagggtata tcggatttat
      361 gcgcattatg agtaatgcaa aaggctttcc atcgattgca tccggtgaaa gcagtcttac
      421 ggggtttatt agtcaggtag atggaacacc agcgtataca ggtgtatttc agggatgggc
      481 tacgcgctcg ctttatactt atcgctggaa tccgacaata ggcccgcagt ggacgcgtca
      541 cgcaagaaaa gatgaagtgg acaggcttac tcaactaaca ggtaatacac gcgtatattg
      601 tcaaaatgca agaaaatttt ttagtatcta cgattcagga acatggggtt attacgatgc
      661 agagaatagc gaatggattc cgctttcgat cgcggccgga ggtactggtg cggcaactat
      721 tgatggtgcc agaacgaatc ttggcttagg taggaacaac agccctcaac ttaacagtct
      781 atttcttgat agatatagtg attcgaccaa tacatacaca tctagtggaa ttctccatac
      841 aagactgtta gcaactgaca gcactgtgcg tcttggtgct gatatgtatg ttgagacttt
      901 atcaaatgag ccaggtcaat taacaataag gtttacatac gatggttcaa ctggtgcttc
      961 aaaatatcta aaccttaact cagaaggtaa tttaattgta gatagtgcga tactgagatc
     1021 aacggttgaa aaacctttac aaataaggag cgctaaccca gcaataagat ttaatgaaac
     1081 agatcgcccg gctaacacgc ctacttatac tctcattgct aatgctgg
//
LOCUS       DAFAVF010000359         1124 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.376, whole genome shotgun sequence.
ACCESSION   DAFAVF010000359 DAFAVF010000000
VERSION     DAFAVF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.376"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..349
                     /locus_tag="LGK42_004650"
     CDS             <1..349
                     /locus_tag="LGK42_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBK2660786.1"
                     /translation="VQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGS
                     ISGYVFEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKF
                     LIRSETDGVEAKK"
     gene            complement(409..1047)
                     /gene="ompW"
                     /locus_tag="LGK42_004651"
     CDS             complement(409..1047)
                     /gene="ompW"
                     /locus_tag="LGK42_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBK2660787.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          317 a          272 c          243 g          292 t
ORIGIN      
        1 tgttcaactg gaaactattc ttgatcgtaa tgacatttca cgttcagtca ttaaagattc
       61 catgagtaaa atggctgcgc ttgggcagtc aatcggtggt atattccctt ctgatgaaat
      121 agttaaaggc tctattagcg gatatgtctt cgagcaattt gaaatcgcct gttacacctc
      181 actattagca gcagcaaaaa atgccggtga tacagtctca attccgatca tcgaagcgat
      241 tttaaatgag gaaaagcaaa tggccgactg gctgattcag catattccgc aaacaactga
      301 gaaattttta attcgttctg aaactgatgg cgtagaagcg aagaaataat accctttata
      361 ccatgtcctt attgaccccg tatattacgg ggtcgttttt gtgcggaatt aaaaacgata
      421 tcctgctgag aacataaaca cccacggatc gaggcgtacg ctatcgtgtt gctgtgcacc
      481 gcctagctta taattggcgg tggtatcgat atccatgtac cacactgaca tgttaaccaa
      541 ccagtcacgg ttaatcagat aatcaacccc cacctgcccg gcagctcccc aggaatcttt
      601 cagactgaga tcggaaagcc ccgcctcttt gccatgatcg ttaaatccat tatcaaagaa
      661 ggtggtgtag ttaataccgg ccccaacgta aggacggaat ttgctgctgg catcaccaaa
      721 ataccactgc gccatcagtg ttggcggcag atggtgaacg gttgcaatat cgccggtcgc
      781 ccgggtgccg attttatggc ggaacggcgt cgctgccagt aattccacac caatgttgtc
      841 ggtcgccata taagtaaacg tcaagcccag ttgagtgtta ttggtcacgc tgaatccacc
      901 cagacttcct aacgtaccac cagcaccttc tgttggacgt acggttgcag aacctgcacg
      961 cataaaaaat tcgcctgctt catgcgcaaa ggcactgcca gaaagaagag ttgttactgc
     1021 caaagccgcc actgttaact ttttcatatc cgctccgtcg ttatggttat aaaaacgtga
     1081 cgaatatacc tacattggag taacaaatga tcctacatag atca
//
LOCUS       DAFAVF010000360         1100 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.378, whole genome shotgun sequence.
ACCESSION   DAFAVF010000360 DAFAVF010000000
VERSION     DAFAVF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.378"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..946
                     /locus_tag="LGK42_004652"
     CDS             <1..946
                     /locus_tag="LGK42_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK2660788.1"
                     /translation="SIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSA
                     VTVNNYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRN
                     QQAKNLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRV
                     IHLIKPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGD
                     HFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMV
                     FQVYGKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(966..1076)
                     /locus_tag="LGK42_004653"
     CDS             complement(966..1076)
                     /locus_tag="LGK42_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBK2660789.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
BASE COUNT          357 a          222 c          235 g          286 t
ORIGIN      
        1 gagcataatc ggtgaaaaaa atcttgcctc atcgattaat aaagaaaaat tactggaggt
       61 tcgtaaagag ttactgactg gataccagat ccccaaaagt aactatattg ttacacaacc
      121 agggagatcg gctgtaactg taaataatta catgacaaat cttaacgccg tgttccagtt
      181 tggtgttgat aacggttacc tggcagataa tccgtttaag gggatctcgc cattaaagga
      241 atcaagaacc attccggatc ctctttcgcg ggaagaattt atccgtctta tcgatgcgtg
      301 cagaaatcag caagcaaaaa atttatggtg tgtttctgtt tatactggag ttcgccctgg
      361 tgagctgtgt gcacttggat gggaggacat agatctgaaa aatggaacaa tgatgatcag
      421 gagaaattta gcaaaagacc gtttcacggt accaaaaaca caggcgggaa ccaatcgggt
      481 cattcatctt attaagccag caatcgacgc tctccggagt cagatgacat taacgagact
      541 gagcaaagag catatcattg atgttcacct cagagagtat ggcagaacag aaaaacaaaa
      601 atgcaccttt gtttttcaac ctgaagtgtc agcgagagta aaaaattatg gtgaccattt
      661 taccgttgac tcaataaggc agatgtggga cgcagcgata aaacgtgccg gactccgcca
      721 tcgaaaatca tatcagtcga gacatactta tgcctgctgg tcgctgacag ctggtgctaa
      781 cccggcattt atagcaaacc agatgggcca tgcagatgcg caaatggtat ttcaggtata
      841 cggaaaatgg atgtctgaaa acaataatgc acaggtagct ttgttaaata cacagttaag
      901 cgagtttgcc ccaaccatgc cccataacga agcaatgaaa aattaattta atatttatca
      961 aatagttaac acgcatgact cttgaaatcc ataaattcaa gcgcagtgcc cagccatccc
     1021 gatactgctg ctttcaccaa atccttagtg cttctttcat gtttttctat tgtcataatg
     1081 gttatctcta aaaaagaggt
//
LOCUS       DAFAVF010000361         1079 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.379, whole genome shotgun sequence.
ACCESSION   DAFAVF010000361 DAFAVF010000000
VERSION     DAFAVF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.379"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..483
                     /locus_tag="LGK42_004654"
     rRNA            <1..483
                     /locus_tag="LGK42_004654"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            569..644
                     /locus_tag="LGK42_004655"
     tRNA            569..644
                     /locus_tag="LGK42_004655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Glu,seq:ttc)
     gene            838..>1079
                     /locus_tag="LGK42_004656"
     rRNA            838..>1079
                     /locus_tag="LGK42_004656"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          294 a          250 c          292 g          243 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgtact ttgcagtgct cacacagatt gtctgataga aagtgaaaag
      541 caaggcgtct tgcgaagcag actgatacgt ccccttcgtc tagaggccca ggacaccgcc
      601 ctttcacggc ggtaacaggg gttcgaatcc cctaggggac gccacttgct ggtttgtgag
      661 tgaaagtcac ctgccttaat atctcaaaac tcatcttcgg gtgatgtttg agatatttgc
      721 tctttaaaaa tctggatcaa gctgaaaatt gaaacactga acaacgaaag ttgttcgtga
      781 gtctctcaaa ttttcgcaac acgatgatga atcgcaagaa acatcttcgg gttgtgaggt
      841 taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa ggacgtgcta
      901 atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt tccgaatggg
      961 gaaacccagt gtgattcgtc acactatcat taactgaatc cataggttaa tgaggcgaac
     1021 cgggggaact gaaacatcta agtaccccga ggaaaagaaa tcaaccgaga ttcccccag
//
LOCUS       DAFAVF010000362         1071 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.380, whole genome shotgun sequence.
ACCESSION   DAFAVF010000362 DAFAVF010000000
VERSION     DAFAVF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.380"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(96..>1071)
                     /locus_tag="LGK42_004657"
     CDS             complement(96..>1071)
                     /locus_tag="LGK42_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK2660790.1"
                     /translation="TDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIRDVTEIN
                     PNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVIGERSER
                     AECFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLGLN
                     TRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANRT
                     RKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMK
                     EDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT          305 a          232 c          238 g          296 t
ORIGIN      
        1 tcttagtgaa tactatagga taaatgataa aaacaatgaa ttattgcaca ggttacaaaa
       61 gacaatcagg gcgaaaccgc cctgatcaga gaagattagt cgctgccaaa gagatctcgt
      121 gtgtagactt tgtctgccac atccttaagc tcttctgcca tacggttgga gataataacg
      181 tcggcttgtt gtttgaaggt cgcgagatca cgttccaggc gagagttgaa gaatgagtct
      241 tctttcatca ccggctcgta aatgataact tcaacgcctt tcgccttgat acgtttcata
      301 atcccctgaa tggaagacgc acggaagtta tctgaaccgc tcttcataat cagacgataa
      361 atacccacca cttgcggctt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg
      421 cggttagcgt ctacgattgc cgagatcagg ttattcggca cagactggta gttcgccagt
      481 aactgcttgg tatctttcgg cagacaataa ccaccataac caaacgacgg attgttgtaa
      541 tggttgccaa tacgcggatc gagacaaacg ccttcgataa tctggcgagt attcagacct
      601 aaactttctg catagctatc caattcgtta aagtacgcca cgcgcatcgc caggtaggta
      661 tttgcaaata gtttaatcgc ttctgcttca gtagagtcgg taaacagggt tgggatattt
      721 tgcttaatcg caccctcctg taataaagca gcaaaacatt ctgcgcgttc tgaacgctca
      781 ccgatgacaa tacgtgacgg atgaaggtta tcgtaaaggg ctttaccttc gcggaggaat
      841 tccggagaga aaataaggtt atcgataccc agacgttctt taattgattt ggtgaagcct
      901 accggaattg tagatttaat caccatgaca gcattaggat taatttctgt cacatcgcga
      961 atgaccgctt ccacgcttga ggtattaaaa taatttgttt tcgggtcata atcggtaggc
     1021 gtggcaataa taacgtaatc ggcattttta tacgcgtcat acttatctgt c
//
LOCUS       DAFAVF010000363         1058 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.383, whole genome shotgun sequence.
ACCESSION   DAFAVF010000363 DAFAVF010000000
VERSION     DAFAVF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.383"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..100)
                     /locus_tag="LGK42_004658"
     CDS             complement(<1..100)
                     /locus_tag="LGK42_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660791.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFN"
     gene            108..613
                     /gene="tnpA"
                     /locus_tag="LGK42_004659"
                     /pseudo
     CDS             108..613
                     /gene="tnpA"
                     /locus_tag="LGK42_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(631..>1058)
                     /locus_tag="LGK42_004660"
     CDS             complement(631..>1058)
                     /locus_tag="LGK42_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBK2660792.1"
                     /translation="STQVGQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGA
                     ITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVV
                     RQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          273 a          258 c          237 g          290 t
ORIGIN      
        1 gattgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac
       61 cgggtcttaa ctggaattta aatgcctgta atcgtttcac aggccaaatg atactttggt
      121 ctatgaataa agaaaccgat atccgacggg gaagcattgt gttttcctga tgcatgttca
      181 cctggtcttt gtcaccagat accgacgtca gatttttgac tatgacgcga cagaaaaact
      241 acgcacttac ttttcaaatg tatgtgctga ttttgaagct gaactggttt aaatggatgg
      301 cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga tatccagtct
      361 ggtaaacagc ctcaaaggcg tatcgagtag gttactgcga cgagatcgac cagatattgc
      421 agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca gtagctgcgg
      481 aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt
      541 ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc accggataaa
      601 aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa acatggtggc
      661 atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg gcgtactttc
      721 gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt tattagtcgc
      781 tgcatcaatc agctcacctt caaaatagag gcgagtatcc atggtacggt ggcctgtagc
      841 catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt gcagcccttc
      901 tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg gcccagcggt
      961 ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg tatagttcaa
     1021 aattttatcc agaactttct gccctacctg ggtagaag
//
LOCUS       DAFAVF010000364         1058 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.384, whole genome shotgun sequence.
ACCESSION   DAFAVF010000364 DAFAVF010000000
VERSION     DAFAVF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.384"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(193..1047)
                     /gene="rclR"
                     /locus_tag="LGK42_004661"
     CDS             complement(193..1047)
                     /gene="rclR"
                     /locus_tag="LGK42_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBK2660793.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          291 a          269 c          240 g          258 t
ORIGIN      
        1 tcacgtatca agtaatagtc ggggttttat ttaattgaag caatttagtt tgcattttta
       61 tttaaaaact gagctagagt ccatcagaaa aaaatatata aagcccaaac caccgttgga
      121 aatagaaata gttgaaaggc tcacaactcc cggaatgcgg attatctttg ccctgatttc
      181 tggcggtttt attcagggtg caagctgtct gaccctttcc cgatattctc ccggagtaca
      241 accaaactcg cggacaaacg ccttgtgaaa agatgattca ctggcatagc ctactgactc
      301 agcgatcacc acaacaggga gcatttcccg ggaaaacatc tgggccgcta tttgtagacg
      361 caactttgtt aatacagcca gcggcgtggt tccggaaaca tcacggaaaa gctgggcaaa
      421 acttgcccgg gacatgtgag cgatgctggc cagcgattcg acggtccagg cgtgtcctgg
      481 catttccagc atttgctgta ttaccgcacc aagacgtgga tgcagaagca aactgagaat
      541 gtttttctca gtattaacct gtgcaatcca ctcacgcacc gcgagggtaa agaatgtagc
      601 gcagatctgg ctacacagtg catccactcc cggcatcgcc gatctggatt cctgttgtaa
      661 aaacgggatc gcctccctca gccagttata ttccacgctg tggttaaccg gtgctaaaaa
      721 cagcgtttcc ggcaaagacg ttaaaaaata acgcgccgaa tgttgcaacc gaagagtgcc
      781 gcagacaata caggtcgatt cattatcgac atgactcagg cgatgagctg aattttgtgg
      841 tagcaggacc acatttcccg ggcgtaatgt aaaaatctct cccgtcggca tttccagctt
      901 cgccgctcct tgcgttaacg catgccaacg aataaccgat aattccccgg caccatgtgg
      961 aagctgccag tcacttccta acacgcaatt cttatcgatc gttccttgtg gagcgttaag
     1021 catcaacaaa cggctaaggg catccatatc agactcct
//
LOCUS       DAFAVF010000365         1040 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.386, whole genome shotgun sequence.
ACCESSION   DAFAVF010000365 DAFAVF010000000
VERSION     DAFAVF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.386"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            22..837
                     /locus_tag="LGK42_004662"
     CDS             22..837
                     /locus_tag="LGK42_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2660794.1"
                     /translation="MTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLR
                     GNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSV
                     LSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTG
                     GKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGT
                     FEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            834..>1040
                     /locus_tag="LGK42_004663"
     CDS             834..>1040
                     /locus_tag="LGK42_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660795.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWK"
BASE COUNT          236 a          246 c          359 g          199 t
ORIGIN      
        1 attgatgcga aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag
       61 cgcctgaagg aacagtatgg cgataatccg ctggcgctga ataacgtcat gtcagagcag
      121 aaaaagacct gggcggctga agaccagctt cgcgggaact ggatggcagg cctgaagtcc
      181 ggctggagtg agtgggaaga gagcgccacg gacagtatgt cgcaggtaaa aagtgcagcc
      241 acgcagacct ttgatggtat tgcacagaat atggcggcga tgctgaccgg cagtgagcag
      301 aactggcgca gcttcacccg ttccgtgctg tccatgatga cagaaattct gcttaagcag
      361 gcaatggtgg ggattgtcgg gagtatcggc agcgccattg gcggggctgt tggtggcggc
      421 gcatccgcgt caggcggtac agccattcag gccgctgcgg cgaaattcca ttttgcaacc
      481 ggaggattta cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgag
      541 tttgtcttca cgaaggaggc aaccagccgg attggcgtgg ggaatcttta ccggctgatg
      601 cgcggctatg ccaccggcgg ttatgtcggt acaccgggca gcatggcaga cagccggtcg
      661 caggcgtccg ggacgtttga gcagaataac catgtggtga ttaacaacga cggcacgaac
      721 gggcagatag gtccggctgc tctgaaggcg gtgtatgaca tggcccgcaa gggtgcccgt
      781 gatgaaattc agacacagat gcgtgatggt ggcctgttct ccggaggtgg acgatgaaga
      841 ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc ggtcccttct gtaagaaagg
      901 tgcgctttgg tgatggttat tctcagcgag cgcctgccgg gctgaatgcc aacctgaaaa
      961 cgtacagcgt gacgctttct gtcccccgtg aggaggccac ggtactggag tcgtttctgg
     1021 aagagcacgg gggctggaaa
//
LOCUS       DAFAVF010000366         1031 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.388, whole genome shotgun sequence.
ACCESSION   DAFAVF010000366 DAFAVF010000000
VERSION     DAFAVF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.388"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            382..788
                     /locus_tag="LGK42_004664"
                     /pseudo
     CDS             382..788
                     /locus_tag="LGK42_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          283 a          247 c          239 g          262 t
ORIGIN      
        1 ctccacaaaa aatagaaaat atatactgcc caggaaattc tgaagcagaa atacaataat
       61 ttcaaaatat tccgctattt gctcccaatg caaaacatat attgcattgg gagtatattc
      121 atattttaga ctttcttgta tgagtgaact gctccgggtt tccttgagag tgttttatct
      181 gtgaaatcgg gctgccaaat cattgttttc gatggaagca taataagctt gttctgctgc
      241 tgtcaaagta atatggccca gtctttccag caatcgtcga ttgttatacc agtccaccca
      301 cgttagtgtt gccagctctg atcttaccca ccaatagtgg acacaggact aagtgagtaa
      361 actcttaacc agaggtgact catgacaaaa ccagtatcaa tcagcaagaa gccccgtaaa
      421 caacatacgc ctgaatttcg taacgaagcc ctgaaacttg ctgaacgcat cggtgtggcc
      481 gccgcagccc gtgaactcag cctgtatgaa tcccagcttt atgcctggcg cagtaaacag
      541 cagcaacaaa tgagttcgtc agagcgcgaa agcgaactgg ccgctgaaaa tgtccgcctt
      601 aaacgacaac tggcggagca ggctaaggaa ctggccatcc tccaaaaggc cgcgacatac
      661 ttcgcgaagc gcctgaaatg aagtatgtct tcatcgaaaa tcatcgggca gagttcagca
      721 tcaaagcgat gtgtcgtgta cttcgggttg cccgcagcgg ctggtatgtc tggctcaggc
      781 gtcgtcacag tgcctgtggc ggtctcagcc cggaacaatt tgaaaaccat aatctcgctt
      841 agggccgtgt ccacattacg tgggtaggat cagtccttta ttttctgcaa agtcgcagta
      901 tgtctggtaa ggattgttgc accaaccgtt gatgcagcag caacggttgg gagtaatgca
      961 gatagtgcca tagaggtaag tactgaaagt ctttttcctc tgcgggtact ccctttacat
     1021 acccttcggg c
//
LOCUS       DAFAVF010000367         1020 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.389, whole genome shotgun sequence.
ACCESSION   DAFAVF010000367 DAFAVF010000000
VERSION     DAFAVF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.389"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(40..>1020)
                     /locus_tag="LGK42_004665"
     CDS             complement(40..>1020)
                     /locus_tag="LGK42_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK2660796.1"
                     /translation="EDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVF
                     YSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSKEQRDRLTG
                     WIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLG
                     VSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVRRVV
                     TLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRG
                     DDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          201 a          331 c          260 g          228 t
ORIGIN      
        1 agcggctcat taaaggccat gctggcaata tgcgggagat tacgcagctc tgctgtcact
       61 cttctcctcc tctgttgatt gtcgcagtcc ggattcaaat gccgcagccg cccaggcggg
      121 cggtttaaga ccggctgcac ggcgctccat cgtttcacgg acctgctggg caaaaatttc
      181 ctgatagtcg tcaccgcgtt ttgcgcactc tttctcgtag gtgctcagtc cggcttctat
      241 cagcatcacc gcttcctgta cttctttcag accatcgatg gccatacgac cggagcctat
      301 ccagtcacag ttcccccagg cgctgcgggc ttcctgaaag ctgaaacgcg cttttgaagg
      361 taacgtcacc acgcggcgaa cgatggcctc ttccagccag cacagaaaca tctggctcgc
      421 ctgacgggat gcgacgaatt ttcgccgccc cataaagtac gcccacgact cgttcgcact
      481 ggcccgtgcc gtggagtagc tcatctgggc gtaattccgg gaaagttgct catacgagac
      541 acccagcccg gcagcgatat accgcaacag tgactgctca aacacggagt agccgttatc
      601 cgtgtcctga gccgtctgca ggttcagtga gtcccccggc atcaggtgcg gcacttttgc
      661 gcctcccagc cggaccggtg ctgcggcgta atacgcggca atttcaccaa tccagccggt
      721 cagcctgtcc cgctgctcct tactgttcgc gccaagaata aaatccatcg ctgactgcgt
      781 atccagctca ctctcgatgg tggcggcata catcgccttc acaatggcgc tctgcagctg
      841 cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc acgctgtaaa acacatttgc
      901 accgcgggtc tgcccgtcct ccacgggttc aaagacgtga atgaacgaag cacgaccgcc
      961 cggtaactca cggggtatcc atgtccattt ctgcggcatc cagccaggat agccgtcctc
//
LOCUS       DAFAVF010000368         1008 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.391, whole genome shotgun sequence.
ACCESSION   DAFAVF010000368 DAFAVF010000000
VERSION     DAFAVF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.391"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            22..837
                     /locus_tag="LGK42_004666"
     CDS             22..837
                     /locus_tag="LGK42_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK2660797.1"
                     /translation="MTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDLLR
                     GNWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSV
                     LSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTG
                     GKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGIN
                     FNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
     gene            834..>1008
                     /locus_tag="LGK42_004667"
     CDS             834..>1008
                     /locus_tag="LGK42_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK2660798.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVL"
BASE COUNT          228 a          239 c          345 g          196 t
ORIGIN      
        1 attgatgcga aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag
       61 cgcctgaagg aacagtatgg cgataatcct ctggcgctga ataacgtcat gtcagagcag
      121 aaaaagacct gggcggctga agacctgctt cgcgggaact ggatggcagg cctcaggtcc
      181 ggctggagcg agtggaaaga gagtgccacg gacagtatgt cgcaggttaa aagtgcagcc
      241 acgcagacct ttgatggtat tgcgcagaat atggcggcga tgctgaccgg cagtgaacag
      301 aactggcgca gcttcacccg ctccgtgctg tccatgatga cagaaattct gcttaagcag
      361 gcaatggtgg ggattgtcgg gagtatcggc agcgccattg gcggggctgt tggtggcggc
      421 gcatccgcgt caggcggtac agccattcag gccgctgcgg cgaaattcca ttttgcgacc
      481 gggggattta cgggaaccgg cggcaaatat gagccagcgg ggattgttca ccgtggtgaa
      541 tttgtcttca cgaaggaggc aaccagccgg attggtgtcg gcaacctgta ccgcctgatg
      601 cggggctatg cggaaggtgg ttatgtgggc ggtgccggaa gtccggcgca gatgcggcgg
      661 gctgaaggca ttaattttaa tcagaacaat cacgtggtga ttcagaacga cggtacgaat
      721 ggtctgccag gtccacagat gatgaaggcg gtgtatgaca tggcccgcaa gggtgcccgt
      781 gatgaaatcc aggcacagat gcgcgatggt ggtctgttct ccggaggtgg acgatgaaaa
      841 ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc ggccccttct gtaagaaagg
      901 tgcgctttgg tgatggctat tcccagcgag cgcctgccgg gctgaatgcc aacctgaaaa
      961 cgtacagcgt gacgctttct gtcccccgtg aggaggccac ggtactgg
//
LOCUS       DAFAVF010000369          996 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.394, whole genome shotgun sequence.
ACCESSION   DAFAVF010000369 DAFAVF010000000
VERSION     DAFAVF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.394"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..861
                     /locus_tag="LGK42_004668"
     CDS             <1..861
                     /locus_tag="LGK42_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBK2660799.1"
                     /translation="SIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLL
                     IALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLEL
                     TDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDF
                     LPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREA
                     TPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQ
                     STSKTAPQAK"
BASE COUNT          298 a          225 c          230 g          243 t
ORIGIN      
        1 agcatcaagg gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg
       61 ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg
      121 cgcgacctgc tgattgcgct ggaaagtcgt atgcatcaaa agcataaact ggatgctgct
      181 gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc
      241 ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg
      301 gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc
      361 aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat
      421 gcaattaata atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc
      481 ctgccgttat atgacgttga ctgccaggaa aatggcaatc tggaatatga cacctattcc
      541 caaccggaat ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa
      601 gatgaaagtg gcaaagaaca atttagcggc gcagtggtga aaacgcgtga agcaacgccg
      661 ggtaaagaaa tagaggcgat cactcgccga atgcttgatt tcagccaacg actgaaaaaa
      721 ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg
      781 ctaacccagg atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa
      841 actgcaccac aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct
      901 gaaatgactg ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc cttatccggc
      961 ctacaaattc tcaagcattc aatcagttgc aatgcc
//
LOCUS       DAFAVF010000370          993 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.395, whole genome shotgun sequence.
ACCESSION   DAFAVF010000370 DAFAVF010000000
VERSION     DAFAVF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.395"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            45..584
                     /locus_tag="LGK42_004669"
     CDS             45..584
                     /locus_tag="LGK42_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HBK2660800.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            581..>993
                     /locus_tag="LGK42_004670"
     CDS             581..>993
                     /locus_tag="LGK42_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK2660801.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSALVKRGIFIQSGRNIG"
BASE COUNT          265 a          235 c          270 g          223 t
ORIGIN      
        1 attctgccgc ctaatctgcg gcgtgaactg ataaggcaat gactatgcaa ccacttacat
       61 accaacagac tagcggattt agcccgactg cggtgataaa tcgttctcaa acaaaacaag
      121 ctccaggcca cgaaaaaatc cgtgatgccg tccgcgcctg gtcggctgca gataatcagg
      181 atgttgttgc cgcactcatt gtgaatgagt atcgggagca gggcggcggc accatcgatt
      241 tccctgatga tgtcagccgt gcacgccaga agctgttccg cttcctcgat aacaaattcg
      301 attctgaaaa ataccgaaat aacgtgcgtg aactgacccc ggcaattctg gcggtactac
      361 cgctggaata tcgcggccac ctggttgagc aggatagctt catggctcgg ctggctgaaa
      421 tggaaaagga actcagtgag gcaaaacagg ctgtcattct caacgcacca cgccaccaga
      481 aactgaagga gatgagtgaa ggcattgtgt cgatgtttcg tgtggacccg gatctggctg
      541 gtccactgat ggcgatggtc accaccatgc tgggggcaat atgacaggtt cggaaatggc
      601 gaaagccggt ctgcgcgaac agaaccgact ttcaggtgca aatcgtaaca cactcattgc
      661 gggaggaatt atggcaaaca ctgctgagat attcaatttt ccagtgccgg atgcggcaca
      721 aaaggagccg cgcgtggcag atctcgatga tggttatacg cgcattgcaa atgagttgct
      781 ggaagctgtg atgctggccg gattaacaca gcaccagctt ctggtctttc tggctgtcat
      841 gcgcaaaaca tatggcttta ataaaaaact ggattgggtt agcaacgagc aactttccga
      901 attaaccggg atattgccgc acaagtgttc tgctgcaaaa agtgctctgg taaagcgtgg
      961 gatttttatt cagagcgggc ggaatatagg cat
//
LOCUS       DAFAVF010000371          964 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.398, whole genome shotgun sequence.
ACCESSION   DAFAVF010000371 DAFAVF010000000
VERSION     DAFAVF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.398"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>964
                     /locus_tag="LGK42_004671"
     CDS             <1..>964
                     /locus_tag="LGK42_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBK2660802.1"
                     /translation="NGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTF
                     LGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGKEQYLKFGD
                     KETPFGLKWMPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSS
                     SGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKI
                     GERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQI
                     IMGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPT"
BASE COUNT          226 a          233 c          292 g          213 t
ORIGIN      
        1 aatgggcgtg gcttctggtg cctgggcggt aaagcggcaa aaaactaccg tgaaaagtca
       61 gtggatgtgg cgggttatga tgaacttgct gcctttgatg aggatattga acaggaaggc
      121 tctccgacgt tcctgggcga taagcgtatt gaaggctctg tctggccaaa gtccatccgt
      181 ggctccacgc ccaaagtgag aggcacctgc cagattgagc gtgcagccag tgaatccccg
      241 cattttatgc gttttcatgt tgcctgcccg cactgcggga aggagcagta ccttaaattt
      301 ggcgacaaag agacgccgtt tggcctcaaa tggatgccgg atgatccctc cagcgtgttt
      361 tatctctgtg agcataatgc ctgcgtcatc cgtcagcagg agctggactt tactgatgcc
      421 cgttatatct gcgaaaagac cgggatctgg acccgtgatg gcattctctg gttttcgtca
      481 tccggtgaag agattgaacc gcctgacagt gtgacctttc acatctggac ggcgtacagc
      541 ccgttcacca cctgggtgca gattgtcaaa gactggatga agacgaaagg ggatacggga
      601 aaacgtaaaa ccttcgtgaa caccacgctc ggtgagacgt gggaagcgaa aatcggcgaa
      661 cgtccggatg ctgaagtgat ggcagagcgg aaagagtatt attcagcgcc cgttcctgat
      721 cgtgtggctt acctgaccgc cggtatcgac tcccagctgg accgctacga aatgcgcgta
      781 tggggatggg ggccgggtga ggaaagctgg ctgattgacc ggcagattat tatgggccgc
      841 cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac ctatacccgc
      901 cggaatggtg cagaaatgtc ggtatcccgt atctgctggg atactggcgg gattgacccg
      961 acca
//
LOCUS       DAFAVF010000372          951 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.400, whole genome shotgun sequence.
ACCESSION   DAFAVF010000372 DAFAVF010000000
VERSION     DAFAVF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.400"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..899)
                     /locus_tag="LGK42_004672"
     CDS             complement(<1..899)
                     /locus_tag="LGK42_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBK2660803.1"
                     /translation="MNAMATKLTIEQRLELLENELSQNKQELKATQNELGVYKSRLST
                     LQKSITENKYKSASLAEISATSPVADNIKNENGEQNSSAAAHTINGSQQVAVIESKGD
                     KTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGSPQTYAAGSLGRFGNEMSGW
                     FDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSANENIMQFSDIYLTTRGFL
                     PFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFDMSLSRDDVD
                     VYSRDFSRTSQMNTNSVDVRYRN"
BASE COUNT          245 a          225 c          198 g          283 t
ORIGIN      
        1 atattgcgat aacgaacatc cacagaattg gtattcatct ggctggtacg cgaaaaatca
       61 cgagagtaaa catcgacatc atcacggctt aaggacatat caaacagccc tacaccaagt
      121 gcccagtttt caatccccac acccgcggcg acatccgtgg ttaaggtttt ccagtccagc
      181 atctggatct catattgcgg gagtttatgt ttgccgaccc agaagtctgc ctctggtgcg
      241 aagggtaaaa aacctcgcgt tgtcaggtaa atatcgctga actgcatgat attttcattg
      301 gcactgtcac caaaccaggc atcgttatac tgctcaccca cgttgccatc ataggtaacg
      361 accgcatttg ccgttttacc gtcctgatta taaacacgct gatttaaggt caggtcaaac
      421 caaccgctca tctcattacc aaaacgtccc agagaacccg ctgcataagt ttgtggtgag
      481 ccgtgattac cagtacccca gcctgagcgg aagtacccct gatagctgaa cccaatatca
      541 tcctttatat atttactgat atctttcagg gtcacgcttt cgatagtggt tttgtcgcct
      601 ttactttcaa taacggcaac ttgctgcgat ccatttatag tatgcgctgc ggcagacgag
      661 ttctgttcac cgttttcatt tttgatgtta tcagcgacgg gagatgtcgc tgatatttca
      721 gcaagcgagg ctgatttata tttgttttcc gtgatgcttt tttgtaaggt cgaaagtcgg
      781 gatttatata ctcccagttc attctgtgtt gctttcagct cttgtttatt ttgcgacaat
      841 tcattttcaa gcaattcaag gcgctgctct atggttaatt ttgttgccat cgcgttcaat
      901 ggaaacaatg ctgtaataat agaaaaagac aaaaggctga cgttaagcgt c
//
LOCUS       DAFAVF010000373          944 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.401, whole genome shotgun sequence.
ACCESSION   DAFAVF010000373 DAFAVF010000000
VERSION     DAFAVF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.401"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>944
                     /locus_tag="LGK42_004673"
     CDS             <1..>944
                     /locus_tag="LGK42_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660804.1"
                     /translation="TCAISQTLEEQPVLNSKSWLTSLQNDYSLPDSLTERIWLTLISQ
                     RISRGELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFRNRLNLSP
                     EANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPENHKPDPEW
                     YCSSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKMFASGGQGHGF
                     AAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQN
                     GQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPG"
BASE COUNT          264 a          226 c          246 g          208 t
ORIGIN      
        1 acctgtgcca tcagccagac actggaagaa caaccggtgc tcaacagtaa atcgtggctg
       61 accagtttgc aaaacgatta cagccttcct gactcactga cggagcgcat ctggctgacg
      121 ctcatttctc aacggatttc ccggggagag ctgagggaat ttgaactggc agacggaaac
      181 tggttactga acaatgcctg gtatgaaaga aacatggcag ggtttaacga acagttgaaa
      241 gagaacctgt cattcacacc tgatgaactg aaaacgctct tccggaaccg cctgaattta
      301 tcaccggaag cgaatgacga ttttctcgat cgttgcctgg acggcggtga ctggtatccc
      361 ttttcagaag gtcgccgttt tgtatcattc catcacgtgg atgagcttcg tatctgtgcc
      421 tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc ataagccgga tccggaatgg
      481 tactgctcct ctctttgtcg cgaaacagaa acactgtgtc aggaaattta tgaacgccct
      541 tacaacagct ttatttccga tgcaacggcg aatggtctga ttctcatgaa actgccggaa
      601 acctggagta caaatgagaa aatgtttgct tccggagggc agggacatgg gtttgccgct
      661 gaacggggaa accatattgt cgacagagtc cgtctgaaaa acgcacggat cctcggtgat
      721 aataatgcca gaaatggagc agacagactg gtcagcggaa cagaaatcca gacgaaatat
      781 tgttcaactg cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg acagtatcgt
      841 tacatgggaa ataatggccc catgcaactg gaagtccccc gtgatcagta tgccggcgct
      901 gttgaaacca tgaggaataa gatccgcgaa ggtaaagtac ccgg
//
LOCUS       DAFAVF010000374          936 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.404, whole genome shotgun sequence.
ACCESSION   DAFAVF010000374 DAFAVF010000000
VERSION     DAFAVF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.404"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>936
                     /locus_tag="LGK42_004674"
     CDS             <1..>936
                     /locus_tag="LGK42_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2660805.1"
                     /translation="QSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHNQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQL
                     TAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAEQWQYDERGWLTDISHIS"
BASE COUNT          231 a          282 c          274 g          149 t
ORIGIN      
        1 cagagtcagt ttgacgccgt gggcaggctc agggcacaga cggatgccgc aggccggaca
       61 acagagtaca gcccggatgt ggtgacgggc ctcatcacgc gcataaccac gccggatggc
      121 agggcatcgg cgttttacta taaccaccac aaccagttaa cgtcagccac cgggcctgac
      181 gggctggaat tgcgccggga atatgatgaa tccggccgtc tgattcagga aaccgcccct
      241 gacggcgata tcacccgcta ccgttatgat aatccacaca gtgacttacc ctgcgcaacg
      301 gaagatgcca ccggcagccg gaaaaccatg acgtggagcc gttacggtca gttgctgacc
      361 ttcactgact gttccggtta tgtaacccgc tatgaccatg accgttttgg tcaggtgacg
      421 gcggtgcacc gcgaggaagg cctgagccag taccacgcat acgacagccg tggacagtta
      481 actgcggtga aagacacgca gggccatgaa acgcggtatg aatacaacgc cgccggtgac
      541 ctgaccgccg tcattgcccc ggacggcagc agaaacggga cacagtacga tgcgtgggga
      601 aaagccatct gtaccacgca gggcggtctg acgcgcagta tggaatacga tgctgccgga
      661 cgggtcatcc gcctgaccag tgaaaacggc agccacacca ccttccgtta cgatgtactc
      721 gaccggctga tacaggaaac cggctttgac ggccgcacac agcgttatca ccacgacctg
      781 accggcaaac ttatccgcag cgaggatgag gggctggtca cccactggca ctatgacgaa
      841 gcagaccgcc tcacgcaccg caccgtgaat ggcgaaaccg cagagcagtg gcagtatgac
      901 gaacgcggct ggctgacaga catcagccat atcagc
//
LOCUS       DAFAVF010000375          936 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.405, whole genome shotgun sequence.
ACCESSION   DAFAVF010000375 DAFAVF010000000
VERSION     DAFAVF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.405"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>936
                     /locus_tag="LGK42_004675"
     CDS             <1..>936
                     /locus_tag="LGK42_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2660806.1"
                     /translation="QSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRREYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDD
                     ATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQL
                     TAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAEQWQYDERGWLTDISHIS"
BASE COUNT          232 a          282 c          275 g          147 t
ORIGIN      
        1 cagagtcagt ttgacgccgt gggcaggctc agggcacaga cggatgccgc aggccggaca
       61 acagagtaca gcccggatgt ggtgacgggc ctcatcacgc gcataaccac gccggatggc
      121 agggcatcgg cgttttacta taaccaccac agccagttaa cgtcagccac cgggccagac
      181 gggctggaaa tgcgccggga atatgatgag tacggacgtc tgattcagga aaccgcccct
      241 gacggcgata tcacccgcta ccgttatgat aatccacaca gtgacctccc ctgcgcaacg
      301 gacgatgcca ccggcagccg gaaaaccatg acgtggagcc gttacggtca gttgctgacc
      361 ttcactgact gttccggtta tgtaacccgc tatgaccatg accgttttgg tcaggtgacg
      421 gcggtgcacc gcgaggaagg cctgagccag taccacgcat acgacagccg tggacagtta
      481 actgcggtga aagacacgca gggccatgaa acgcggtatg aatacaacgc cgccggtgac
      541 ctgaccaccg tcattgcccc ggacggcagc agaaacggga cacagtacga tgcgtgggga
      601 aaagccatct gtaccacgca gggcggtctg acgcgcagta tggaatacga tgctgccgga
      661 cgggtcatcc gcctgaccag tgaaaacggc agccacacca ccttccgtta cgatgtactc
      721 gaccggctga tacaggaaac cggttttgac ggccgcacac agcgttatca ccacgacctg
      781 accggcaaac ttatccgcag cgaggatgag gggctggtca cccactggca ctatgacgaa
      841 gcagaccgcc tcacgcaccg caccgtgaat ggcgaaaccg cagagcagtg gcagtatgac
      901 gaacgcggct ggctgacaga catcagccat atcagc
//
LOCUS       DAFAVF010000376          932 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.406, whole genome shotgun sequence.
ACCESSION   DAFAVF010000376 DAFAVF010000000
VERSION     DAFAVF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.406"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(159..899)
                     /locus_tag="LGK42_004676"
     CDS             complement(159..899)
                     /locus_tag="LGK42_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBK2660807.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
BASE COUNT          260 a          204 c          202 g          266 t
ORIGIN      
        1 tcaggaaggt atggtcatat tcaacaagtt cagcacagaa agcactattg caatacagca
       61 gcgcgcaaaa cgtagcgata cgggtcagat gattatgata atttttagtt ggttttatag
      121 tcacgttatg cttcctgcgg aattatatcc ttgcctgatt acagactggc attaccttca
      181 atggcgccac caaaatcatt aatggcgtaa aaatgtactt ttgcagaact tgctttgcca
      241 ttaaggccag tgactttcat gacctcatca ctaaatggtg caatcatttt cacatcaatc
      301 ggatagcgtt taccgctagc ttctaaatca ccactgctaa aagagacgta gtaaggggtt
      361 ggattggtca ctcgcaatga cgccttacct tctgaacctg accagaacca cttcagggct
      421 aacggggctt cagagggatt tcccttcaat ccatccgggc gatagaaaag ttttatacgt
      481 gtgcgaaatg ccagttgcag cagactttga ttcgcgacct tttctgcatc tggttttggt
      541 ggaacttcca gtacgttaaa ccagaacacg ctctctctgt ctttaggcag tgaggtgctg
      601 gctgtgtaca ttaatttgat tgtttgccca cgtttggcat caatacgcga tactggcggc
      661 gtggcagtaa aagggactgt aatactgcca ggctcagcgt tgtcatcgcc agtatctaac
      721 caactctgga caagcaacgg gttattccct ttattttcca gacgtacgtt gacacttttt
      781 tgatcgcttt tatatattac gcgagtaccc gaaatgacaa tgtccgcaat agacgatgaa
      841 ctaaaagcca tacaggttac gaagcataaa gctgttgtat gtttggtgtt aaaaaacata
      901 aaccatccct gacagagtta attaaaacag gg
//
LOCUS       DAFAVF010000377          920 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.408, whole genome shotgun sequence.
ACCESSION   DAFAVF010000377 DAFAVF010000000
VERSION     DAFAVF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.408"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            302..904
                     /gene="fimB"
                     /locus_tag="LGK42_004677"
     CDS             302..904
                     /gene="fimB"
                     /locus_tag="LGK42_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HBK2660808.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          274 a          170 c          201 g          275 t
ORIGIN      
        1 tacagaacat atcacatgat atatagataa aattagttgc attaatgatg agggttatta
       61 ttcgattcgt atcagattta taaatatata aaggtacata gcatgcaaga gcatggcgtt
      121 tgtatggcaa agttattata agtaactatc gctactccat ttaagttcac tcagaagaac
      181 tggtccactt acgttagtta ttaagcaaac gttcgctttt ataaacataa tcaggattaa
      241 aatgttggat tattgctaac ccagcacagc tagtgcgcgt ctgtaattat aagggaaaac
      301 gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg aaatcgaatc
      361 actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct
      421 ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga
      481 tcttaaggca aagtgtatct atatccatcg attaaaaaaa ggcttttcaa caacgcaccc
      541 gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta cttcgtaccc
      601 gcatgctgag agcgaatggg tatttttatc gcgtaagggg aatccgcttt ctcggcaaca
      661 gttttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc
      721 gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact
      781 tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa
      841 tgcagggcgt ttttacggca tctgggatag ggccagagga cgacagcgtc acgctgtttt
      901 atagagatag ttgagatacc
//
LOCUS       DAFAVF010000378          907 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.409, whole genome shotgun sequence.
ACCESSION   DAFAVF010000378 DAFAVF010000000
VERSION     DAFAVF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.409"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            463..638
                     /locus_tag="LGK42_004678"
                     /pseudo
     CDS             463..638
                     /locus_tag="LGK42_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(696..>907)
                     /gene="iss"
                     /locus_tag="LGK42_004679"
     CDS             complement(696..>907)
                     /gene="iss"
                     /locus_tag="LGK42_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBK2660809.1"
                     /translation="TAVTPKETITHHFFVSGIGQEKTVDAAKICGGAENVVKTETQQT
                     FVNGLLGFITFGIYTPLEARVYCSQ"
BASE COUNT          257 a          192 c          202 g          256 t
ORIGIN      
        1 atgctgttat ttagggacaa agaaccgact ttttctcttg cctcctcgcc agtaatgatt
       61 aatgggaatg cttcctctgg ggaggaggac agtagaaaaa agaacccgcc agcaaaaata
      121 ttggggatga acagctttcg ctactcaggt tgttggcggg taaatttcct catgaaataa
      181 gaatgctacg cgctcttttt aatgaaaatg ataatcactg tcaactaatt gcgcatattt
      241 tcttcataca tgattgataa aggtgattca gactatcagt gttttgctga tggccttttt
      301 gtctttagaa aacaccagcc aggctggttg gttcagttaa gctttcagct tcgagaattt
      361 tatgaaagct attgatttgt taaaataaat cacagtctgg tgactgcaaa agttaaacga
      421 gaaatcaaaa ggtggcccaa atctgaaaat gaagctctgc gtgatgatgt tgccgctggt
      481 cgtcgtcggt tgcatatcag atcaatctgc ctatccccgc gtgaagccac caccgcctcg
      541 ggcgtggata atgcagcccc cccgactggc agacatcgct gaacaggatt atttcctccc
      601 tcagagagag gcttatcact atgcaaaaac aactgtaggg agcccagaag tatattaatg
      661 aacagtgcag atgagctccc catatcgatg ggcaactatt gtgagcaata tacccgggct
      721 tccagcggag tatagatgcc aaaagtgata aaaccgagca atccatttac gaatgtttgc
      781 tgagtttctg ttttaacaac attttctgca ccgccacaaa ttttggctgc atcaacagtt
      841 ttctcttgtc caattcccga aacgaagaaa tgatgagtga tggtttcctt tggtgttact
      901 gctgtcg
//
LOCUS       DAFAVF010000379          904 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.410, whole genome shotgun sequence.
ACCESSION   DAFAVF010000379 DAFAVF010000000
VERSION     DAFAVF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.410"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..841
                     /gene="ag43"
                     /locus_tag="LGK42_004680"
     CDS             <1..841
                     /gene="ag43"
                     /locus_tag="LGK42_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBK2660810.1"
                     /translation="IARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSS
                     VDVKDDDGSRVGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTDNNDFRA
                     RGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRA
                     GFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTS
                     TAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLN
                     VTF"
BASE COUNT          184 a          254 c          282 g          184 t
ORIGIN      
        1 tattgcccgt ggggccacgc cggaaagcag cggcagctat ggattcgtcc gtctggaggg
       61 tgacctgatg agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc
      121 tggccattct tccgttgatg ttaaggatga tgacggctcc cgtgtcggca cggtccggga
      181 tgatgccggc agcctgggcg gatacctgaa tctgatacac aacgcctccg gactgtgggc
      241 tgacattgtg gccctgggaa cccgccacag catgaaagcg tcaacggaca ataacgactt
      301 ccgcgcccgg ggttggggct ggctgggttc actggaaacc ggtctgccct tcagtatcac
      361 tgacaacctg atgctggagc cacaactgca gtatacctgg cagggacttt ccctggatga
      421 cggtaaggac aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc
      481 cggtttccgt ctgggcagcc acaacgatat gacctttggc gaaggcacct catcccgtgc
      541 ccccctgcgt gacagtgcaa aacacagtgt gagtgaatta ccggtgaact ggtgggtaca
      601 gccttctgtt atccgcacct tcagctcccg gggagatatg cgtgtgggga cttccactgc
      661 aggcagcggg atgacgttct ctccctcaca gaatggcaca tcactggacc tgcaggccgg
      721 actggaagcc cgtgtccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag
      781 cgtcagcggc agcagcgctg aagggtataa cggtcaggcc acactgaatg tgaccttctg
      841 acagaaccat cgcctctctg tggtcccggt catcatgacc gggacccgga ccggcgcaac
      901 ggat
//
LOCUS       DAFAVF010000380          900 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.411, whole genome shotgun sequence.
ACCESSION   DAFAVF010000380 DAFAVF010000000
VERSION     DAFAVF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.411"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            170..670
                     /locus_tag="LGK42_004681"
     CDS             170..670
                     /locus_tag="LGK42_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBK2660811.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            762..>900
                     /locus_tag="LGK42_004682"
     CDS             762..>900
                     /locus_tag="LGK42_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660812.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GK"
BASE COUNT          274 a          183 c          206 g          237 t
ORIGIN      
        1 agatacagac cgtaaataaa aacatcttcc ctggtcagga aagaaatctt gaaagcatat
       61 ctggctacga caaaatcgtt gtcaaatata ataactggat caccaaagat aatggcgagt
      121 ttgaactggc ggtccattaa ttatcattgt aataaacgaa ggaatagtaa tgaaaaaggt
      181 atttgcaaaa tctcttctgg tcgcagcgat gttttctgtt gcaggctccg cattggcagt
      241 acaaaaggat attactgtaa cggccaacgt tgatgccgct ctggatatga cacagaccga
      301 taacaccgcg ttgccgaaag cagtagaaat gcaatatctg ccggggcagg gtctgcaatc
      361 ttaccagttg atgaccaaaa tctggtctaa cgacgttacc aaagatgtaa aaatgcagtt
      421 agtttccccg gcgcaactgg ttcaaagcct ggatgctagc aagattgttc cgctgacagt
      481 aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc acttttaccg caaccaaaat
      541 ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa aacctgatgt ttgctcaaac
      601 caccaaaggt gttctggaga caggcatcta ccgtggtgta gtaagcattt atctgtctca
      661 ggatatctaa tccagataac agaattaaca cgtaacaaag ggaggggtag ccctccctta
      721 ttttaactgt tttagtattt gttatggatt tcaggattgc catggataaa aaattactgg
      781 ctcttttgat cctggcgagt ctcagcccgg cagaggcgac attaaccaaa attcccgcag
      841 ggtttgaggt tattgctcag ggacagcagg agtatatcga ggtttatttt tcagggaaaa
//
LOCUS       DAFAVF010000381          894 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.413, whole genome shotgun sequence.
ACCESSION   DAFAVF010000381 DAFAVF010000000
VERSION     DAFAVF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.413"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(318..>894)
                     /locus_tag="LGK42_004683"
     CDS             complement(318..>894)
                     /locus_tag="LGK42_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBK2660813.1"
                     /translation="WAADNAPVAAQQQTQQVQQTQKTAAAERISEQGLYAMRDVQVAR
                     LALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLNDDQYIVINASVGISES
                     YVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMPLKQTRNALADAQK
                     LLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          221 a          195 c          191 g          287 t
ORIGIN      
        1 atatgataaa tattgattcg tggtaatcag agagagtcgc gacatttgtg atgttattct
       61 tacaactgta agaagcgccg gtgatttaca agtaagggta tgattacgct ttttaaagcg
      121 taccataaaa cataacataa taaatgattt gtgatgacac gggattattg ggatttactt
      181 tgaggaagat aaaatgcgtg tttatagaac cgcgagcgcc aaataatttt atgaccacaa
      241 cctgtcgtat tgtggtcata aaaaattgtc ggctggaggg agttaaatcc tttacctcaa
      301 tatactgcct gtcagcatta gttcacaaac agtgcttcgc tgtcgacgat gattccatct
      361 tccgcacctt taagcgccag gtttgcctcg tagtattgct ttttatcaag cagtttctgc
      421 gcatcagcaa gtgcattacg agtctgttta agcggcatca gatattggtt ttccattacg
      481 ccaacaccgg ccaggcgaag ttcttccatt gcgccctttt tatccccttt cgccattttt
      541 tcattggcaa tttttatcgc ggcttctttt tcgggagttg ctacataact ctcagaaatt
      601 ccgacagatg cattaatgac aatatattgg tcatcattga gattggtctt tttacctggc
      661 ttagcgaact ttgcccattc ggtactgtca tctgacagca aagcggaagc ttcattggtc
      721 agttcttttg ccttttctgg atcgccatga aacagagcca ggcgcgctac ctgcacgtca
      781 cgcatcgcat ataaaccttg ttcagagata cgctctgctg ctgccgtttt ctgggtttgc
      841 tgaacttgct gagtttgctg ttgtgccgct accggtgcat tatcagcagc ccat
//
LOCUS       DAFAVF010000382          883 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.414, whole genome shotgun sequence.
ACCESSION   DAFAVF010000382 DAFAVF010000000
VERSION     DAFAVF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.414"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(65..436)
                     /locus_tag="LGK42_004684"
     CDS             complement(65..436)
                     /locus_tag="LGK42_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK2660814.1"
                     /translation="MESYSLTLDEACQFLKISRPTATNWIRTGRLQATRKDPTKPKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEMKYGMPRIRSRAGNELRNLL
                     AQRTSGKLRSCTTSEKQNSGE"
     gene            complement(408..686)
                     /locus_tag="LGK42_004685"
     CDS             complement(408..686)
                     /locus_tag="LGK42_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBK2660815.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            complement(734..>883)
                     /locus_tag="LGK42_004686"
     CDS             complement(734..>883)
                     /locus_tag="LGK42_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBK2660816.1"
                     /translation="RLNHQAISATHCCECGDPIDERRRLAVQGCRTCASCQEEIELKN
                     KQWGL"
BASE COUNT          183 a          210 c          218 g          272 t
ORIGIN      
        1 ttttcctcaa gccatcttag gcaggcctct tcaaaagtga catcaggtaa atcccctagc
       61 ttttctactc gccagagttc tgcttttcgc ttgtcgtgca actcctgagc ttgccgcttg
      121 tcctttgtgc caagagattc cttaattcgt ttcccgcccg ggagcgaata cgaggcatac
      181 catatttcat ttctgcggaa gagtgacatt ttctttcctc tgttatgcca tcacccgcgc
      241 tcacctggac agtatgcagc ggagactgaa gcgccgcaat gcaggcttgt cgcgttgtga
      301 ggtaaggaga ttttggttta gtggggtctt tgcgtgttgc ctgtaggcgg cctgttcgta
      361 tccagttggt agcggtaggt ctggatatct tgagaaactg acaggcctca tcgagtgtga
      421 ggctgtatga ttccatggtt acctctgctt tttgaacgca tgtcacgtaa cttcttaatg
      481 tgttctgccg tttcgatctc ttctgctatc cgatctgcat cagctttatt cacaggttca
      541 aagtcatgat taaagcggaa catgctggcg atacatgttc tgccttttcg gatgtagtga
      601 actttgttgt gggtagaacg caggattttg cagggagtgc cgtggtggtc gacgtaccag
      661 gtgttaggaa aaatgattct gaacattttt acacctcaat tggacgatgt tgaaatttgc
      721 tgctttgagg ccatcacagt ccccattgtt tgttcttaag ttcgatctcc tcctggcaac
      781 ttgcacaagt ccgacaaccc tgaacggcca ggcgtcttcg ttcatctatc ggatcgccac
      841 actcacaaca atgagtggca gatatagcct ggtggttcag acg
//
LOCUS       DAFAVF010000383          829 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.423, whole genome shotgun sequence.
ACCESSION   DAFAVF010000383 DAFAVF010000000
VERSION     DAFAVF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.423"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(92..763)
                     /gene="queE"
                     /locus_tag="LGK42_004687"
     CDS             complement(92..763)
                     /gene="queE"
                     /locus_tag="LGK42_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBK2660817.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGSASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          210 a          213 c          187 g          219 t
ORIGIN      
        1 aaaatggagg agttttcaga ggcgacaaac aatataaatg agtagaagtc tttacaggtt
       61 ccacccattt ataacgctta taaatgttta atcaggcaat atttagatat ttatgtgttt
      121 gcatcgacaa acgccaatta cgcgcaatgc aggtttcaat gcacaaacgt gtggcatcat
      181 ccttttggct aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca
      241 atagttcatc cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg
      301 ctcgctccag tgcctgtgac aacacgtcat agccgccgcg catgttcagc tttggcgata
      361 cggtaaccca ggtattcggt gtgcagcgta cctcatgagt accactggtt tcgatctggc
      421 agctaaaacc gttcttttcg agcagatcag tcagtggtag caaatcatga atgcaaggct
      481 caccacccgt aatcaccaca tgccgcgcgg tgtatccctg gcgaccaatg acagccagca
      541 aatcttcact gctcgccgac ccccacttat cactctcttt ggtcttcgcc agaatgctga
      601 aaagggagac ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac
      661 agccaaccgg gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct
      721 caccttgcag ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt
      781 ggataattgt taattattgc agatcctgcc gcaacaatca tgtcttatt
//
LOCUS       DAFAVF010000384          805 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.425, whole genome shotgun sequence.
ACCESSION   DAFAVF010000384 DAFAVF010000000
VERSION     DAFAVF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.425"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..132
                     /locus_tag="LGK42_004688"
     CDS             <1..132
                     /locus_tag="LGK42_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HBK2660818.1"
                     /translation="SPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            129..>805
                     /locus_tag="LGK42_004689"
     CDS             129..>805
                     /locus_tag="LGK42_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBK2660819.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAK"
BASE COUNT          220 a          215 c          221 g          149 t
ORIGIN      
        1 agtccggcca aactccgcga caagtggacc cagctcgaaa tcaaccgaaa caagcaacag
       61 gctggcgtga cagccggcaa accaaaactc gacctgacga acactgactg gatttacggg
      121 gtggatttat gaaaaacatc gccgcacaga tggttaactt tgaccgtgag cagatgcgcc
      181 ggatcgccaa caatatgccg gaacagtacg acgaaaagcc gcaggtacag caggtagcgc
      241 agatcatcaa cggtgtgttc agccagttac tggcaacttt ccctgcgagc ctggctaacc
      301 gggatcagaa cgaactgaac gaaatccgcc gccagtgggt tctggctttc cgggaaaacg
      361 ggatcaccac aatggaacag gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac
      421 cattcctgcc atcacccggg cagtttgttg cctggtgccg ggaagaagca tccgttaccg
      481 ccgggctgcc aaacgccagc gagctggttg atatggttta cgagtattgc cggaagcgtg
      541 gcctgtatcc ggacgcagag tcttatccat ggaaatcaaa cgcgcactac tggctggtta
      601 ccaacctgta ccagaacatg cgggccaatg cgcttactga tgcggaatta cggcgcaagg
      661 ctgccgatga actgacctgt atgacagcgc gaattaaccg tggtgagacg atacctgaac
      721 cagtaaaaca acttcctgtt atgggcggta gacctctaaa tcgtgcacag gctctggcga
      781 agatcgcaga aatcaaagct aagtt
//
LOCUS       DAFAVF010000385          779 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.427, whole genome shotgun sequence.
ACCESSION   DAFAVF010000385 DAFAVF010000000
VERSION     DAFAVF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.427"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..297)
                     /locus_tag="LGK42_004690"
     CDS             complement(<1..297)
                     /locus_tag="LGK42_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660820.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEP"
     gene            complement(327..779)
                     /locus_tag="LGK42_004691"
                     /pseudo
     CDS             complement(327..779)
                     /locus_tag="LGK42_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          190 a          232 c          128 g          229 t
ORIGIN      
        1 tggctcccac cctcccgaac ctcctgtact gccactgccc aaacagttct tctcttcatc
       61 cgtcaaatcc ttcccgacat ttggattctc ttttgatgaa tcatcatttc caaacacgct
      121 ggaaaaagca ccatagggat ctcgccagaa ttccatttga ctttgtgcat ttaataattc
      181 ggcttcaccc cgcatctcca tacttttctc aatcgcatcc ccaacagcct cgcgattgtt
      241 gtatattact ataccaccac caataaccac cgctccaact gccgcaccga ctaacatccc
      301 tggagggcct cctacccagg gcaagattac gaaagcccgc tccccgcaag gactgacgcc
      361 agatagttcc tgtccatggc tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc
      421 ttatcattcg tcaaaatatt aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca
      481 tttcctcttc ttattttgca gtcgtcttca ttcattacca cgtccagacg ccagtgcagc
      541 ttattctcca cgtgccagtg gtttcggatt gctgtggcga acttctctgc ggttaaatca
      601 gcagaactga tataatatct gaccgtcaat ttctggctct ttcttttgtt ctgctattat
      661 agaccgaaag gagactgcca cgcataattt cttcagccct ttccattcaa acgtgaaatc
      721 aataagttca tcagggacat cgcaaacaat atgaagacgg atttcttctc tgccgtgac
//
LOCUS       DAFAVF010000386          761 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.429, whole genome shotgun sequence.
ACCESSION   DAFAVF010000386 DAFAVF010000000
VERSION     DAFAVF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.429"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            50..742
                     /locus_tag="LGK42_004692"
     CDS             50..742
                     /locus_tag="LGK42_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK2660821.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVSHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT          225 a          161 c          193 g          182 t
ORIGIN      
        1 agcaaaacgt gttttgacca tcaatacgca agcgtataaa ttaaaactta tgaaaaaaga
       61 aactcttgct gatcgcttaa acctagcgat ggaacaatct ggaatgtctc aaggcgctct
      121 tgcaaaggcg tctggcgtag ctcaacccac aatctggaga ctgacaagcg gcaacgcgcg
      181 cggctcaaca aaaattgttg aaatagctaa tgcattgggt gttcgaacag aatggctctc
      241 atcaggcata ggcccgatga gaaatgacgg tcaacaatca gggaagcctg ctgtcagcca
      301 ttccaaatac ttcaaaattg acgttcttga tatagaagtc agtgccgggc cgggagtcat
      361 caaccgtgag tttgtagaag ttctacgctc ggttgagtac tcgtttgacg atgctcgtca
      421 catgttcgat ggcaggaagg cagaaaatat ccgcatcatt aacgtgcgtg gtgacagcat
      481 gtcaggaacg atcgaaccag gtgatctgct gttcgttgat atcacggtta aatctttcga
      541 cggtgatggt atctatgcat ttctgtacga cgacaccgcc catgtaaagc gcctgcaaat
      601 gatgaaggat aaactgctgg ttatctctga taacaagagc tactcaccgt gggaccctat
      661 cgagaaagac gagatgaacc gggtgtttat cttcgggaaa gttattggga gcatgccgca
      721 gacctacagg aagcatgggt aggtagtatg taaagaatta c
//
LOCUS       DAFAVF010000387          753 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.430, whole genome shotgun sequence.
ACCESSION   DAFAVF010000387 DAFAVF010000000
VERSION     DAFAVF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.430"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>753
                     /locus_tag="LGK42_004693"
     CDS             <1..>753
                     /locus_tag="LGK42_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK2660822.1"
                     /translation="KMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDA
                     VGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSGLEYFDLYLLH
                     QAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFA
                     QPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGV
                     VVIPKSTHQQRIEENFAIWDFSLTEKEMAQIS"
BASE COUNT          218 a          145 c          185 g          205 t
ORIGIN      
        1 tgaaaatgcc gatgatggga tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac
       61 agtcagtgtt gaacgctatc cgcacaggtt atcgactcat cgacactgca gcggtatacg
      121 gtaatgaaga tgccgtcggt gaagccgttc gtgaagctat atctgaaggt ttatgtaccc
      181 gagatgagtt atttataacg tctaaattgt gggtgcagga tatgttgaat caggatacgg
      241 cagcagcagg tattgaagca tcattaaaaa aatcgggact agagtacttc gacctctatt
      301 tattgcacca ggctatgcgc gattatttca gtgcgtggcg tgcactcgaa gatgcctatg
      361 aagagggcaa attaaaagca attggggttt ccaatttcta tcctcatgtt ctggcgaact
      421 tttgtgaaac ggtaagagtt aaaccgatgg tcaaccaggt cgagttgcat ccctattttg
      481 cccaaccaga ggcgctggca accatgaagt attataacgt gcagcctgaa gcatgggctc
      541 cgttaggtgg tggacgacat aaaccctttg aaaataatct gcttcagagt attgcagatg
      601 cccatcaaaa atcgatttct caagtcattc tgcgttggaa tattcaacgg ggagtggtcg
      661 ttattccgaa atcgacacat caacagcgta tcgaagaaaa ttttgctatc tgggatttct
      721 cactgacaga gaaagaaatg gcacaaatta gtt
//
LOCUS       DAFAVF010000388          752 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.431, whole genome shotgun sequence.
ACCESSION   DAFAVF010000388 DAFAVF010000000
VERSION     DAFAVF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.431"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(56..>752)
                     /locus_tag="LGK42_004694"
     CDS             complement(56..>752)
                     /locus_tag="LGK42_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HBK2660823.1"
                     /translation="DENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTP
                     TSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNV
                     IGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPIS
                     NMQGTPEKPSDIR"
BASE COUNT          211 a          171 c          142 g          228 t
ORIGIN      
        1 caaataaaag aaaaataaaa aaatagtaac aagcatgctt tcatacatct acaacctatc
       61 ttatatcact gggtttttct ggcgtacctt gcatattaga aatggggtgc ccagatggcc
      121 catatgttat ccaattacca aggtcttttg ttgttatagc cattagatcc ttatttgaat
      181 taaggttcgt tggtgctgtt agcactgtat ccatttttaa cttaccatta gttcctcgcc
      241 cccaccacgt ccatagcgtg tcaggtccta ttacatttgt atgtaactct ttagctaatt
      301 ggctagcaaa tgaattttgt cctttacctg tattacaaga aaataaccaa actgtcatcc
      361 catctttata attcccatca aatttaatta gatatgctag atctttagct gtcatgatac
      421 tgcgcgttgc ggattcaata gatgtggggg taccatgccc cccgattgtg aaaacgcctg
      481 ggatattaat ctcatcagct acagaatgga taagatcact ttcggggtat aaatttatat
      541 caaccaaacc ttgcggatct acatttatga ccggattcaa cggatactga taaaaattcc
      601 atcccccctt cagcccaatc ggatcctgag tgatataccg cccctgcagc gggtcataat
      661 agcggtggcg gttgtaatac aggccggact cctcatcata ctgctgaccc ggcaggcgaa
      721 tgagctgctg cagatgatgc gggttctcat ca
//
LOCUS       DAFAVF010000389          743 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.433, whole genome shotgun sequence.
ACCESSION   DAFAVF010000389 DAFAVF010000000
VERSION     DAFAVF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.433"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..640)
                     /locus_tag="LGK42_004695"
     CDS             complement(<1..640)
                     /locus_tag="LGK42_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBK2660824.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAA"
BASE COUNT          138 a          232 c          191 g          182 t
ORIGIN      
        1 acgctgccgc attcgtttct gacgttttcg ccgcaccggc actggtggcc gccgcgcttt
       61 ttgaggactc tgcagcggca gcactttttg atgcttcagt agcctttgtt gatgccgttc
      121 ctgcgctgga agacgctgac tgagccgacg acgcggcctg tccggctgac gtgctggctg
      181 cgcgtgctga gtctgcagca tcagtcgcat gggttgccgc ctcacgggct gatgtgccgg
      241 catcgccggc tgacttcttc gcggctgccg tgttctgtgc caccgcggac gcgttacgcg
      301 ccacctcttc caccatcagc tcaaaacggc gcagtgcctc cggacggaca tcatcctccg
      361 tcatggcacc aagaaaatca ttcagcgtac cgggttgaga atcttcatac acggtgatgg
      421 tcccggcatg tgacggcggg aacccttcca ccaacagaat aacgctgtac tgaccgtact
      481 caacgtccat gctgtaacgc ccggcttcat ccggattttc agatgccagc gtgttcacca
      541 ccaccgtggt gctgttacgt tttgctttca gctggattgt gcagttctgt accggttttc
      601 ctgtgccgtc tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc
      661 cgcctgaacc ggcgggctgt cataacactg tgttacctgg ctaatcagaa cttataaccg
      721 acacccatga tgaaaccgtc agt
//
LOCUS       DAFAVF010000390          687 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.439, whole genome shotgun sequence.
ACCESSION   DAFAVF010000390 DAFAVF010000000
VERSION     DAFAVF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.439"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            138..230
                     /locus_tag="LGK42_004696"
     tRNA            138..230
                     /locus_tag="LGK42_004696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:172..174,aa:Ser,seq:gct)
     gene            234..310
                     /locus_tag="LGK42_004697"
     tRNA            234..310
                     /locus_tag="LGK42_004697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:268..270,aa:Arg,seq:acg)
     gene            508..584
                     /locus_tag="LGK42_004698"
     tRNA            508..584
                     /locus_tag="LGK42_004698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:542..544,aa:Arg,seq:acg)
BASE COUNT          182 a          162 c          199 g          144 t
ORIGIN      
        1 taattgaacg tgaagtgtgc aaacgataaa agtgtaggaa aaattgtttg acttataagt
       61 ctcagaaagt aatatgtgcg ccacgcagcg acgatgagcg ataaacaagt tcttcgaagc
      121 actcgtaaga ggcgtgtggt gaggtggccg agaggctgaa ggcgctcccc tgctaaggga
      181 gtatgcggtc aaaagctgca tccggggttc gaatccccgc ctcaccgcca tttgcatccg
      241 tagctcagct ggatagagta ctcggctacg aaccgagcgg tcggaggttc gaatcctccc
      301 ggatgcacca tattctacgt actttcagcg atgaaggtat ggaagaggtg gcggtataac
      361 cgcaggcacc agggaggata acgttgcttt agcaacggcc cgaagggcga gccgcaaggc
      421 gagtaatcct cccggatgca ccatctctta cttgatacgg ctttagtagc ggtatcaaaa
      481 aatctgcagt aaagtaagtt tcccgatgca tccgtagctc agctggatag agtactcggc
      541 tacgaaccga gcggtcggag gttcgaatcc tcccggatgc accatattct ccgtaatctt
      601 cagcgatgaa ggtatcgaag aggtggcggt actaaccgca agcaccaggg aggataacgt
      661 tgctttagca acggcccgaa gggcgag
//
LOCUS       DAFAVF010000391          682 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.440, whole genome shotgun sequence.
ACCESSION   DAFAVF010000391 DAFAVF010000000
VERSION     DAFAVF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.440"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..236)
                     /locus_tag="LGK42_004699"
     CDS             complement(<1..236)
                     /locus_tag="LGK42_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660825.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPL"
     gene            complement(353..>682)
                     /locus_tag="LGK42_004700"
     CDS             complement(353..>682)
                     /locus_tag="LGK42_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBK2660826.1"
                     /translation="HAIGDRPVIIFNSRFDIRILKQTAAAHSDPADWLEEMTVYCAME
                     LAAGYYGATNRYGTISLASAASQAGLTWEGQAHSAIADARMAAGVVNAIAAYHLELLQ
                     EQARLKI"
BASE COUNT          156 a          183 c          168 g          175 t
ORIGIN      
        1 agcggggtta aatctggatc gggatcatag gcaatggtag caaaaataaa gcgcagcatt
       61 tctgatgtct tgtaacgctt tacgctggca gccaggcgtg cggcacgttg cggtgcttcc
      121 cagcggtcgt tactgtaatt agccacttca gccagataat ccttttcaag tttttcagcc
      181 gtgtagcacc tgccgcctaa aaaaagccgc atggcctccc tggttttatt tctcacatat
      241 catccctcat taatgctaat gtacgcatac attagcatta acatactgac agtaacaagc
      301 ggcggattta cgtctggata acgtaaatga ttgcggtata caggccagtc ggtcagattt
      361 tcagccgggc ctgttcctgc agcagttcca gatgatatgc agcaatagcg tttaccaccc
      421 ctgccgccat ccgtgcgtcg gcgatcgctg agtgtgcctg cccttcccag gtcagtccgg
      481 cctggctggc agcactggcc agtgaaatcg tgccatagcg gttggtggcc ccataatatc
      541 ctgcagccag ctccatcgca caatataccg tcatttcttc cagccagtca gccggatcgc
      601 tatgtgcggc cgcagtctgt ttcagaatgc ggatgtcgaa ccgggaatta aagataatta
      661 ctggtcgatc tccgattgca tg
//
LOCUS       DAFAVF010000392          670 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.443, whole genome shotgun sequence.
ACCESSION   DAFAVF010000392 DAFAVF010000000
VERSION     DAFAVF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.443"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>670
                     /locus_tag="LGK42_004701"
     CDS             <1..>670
                     /locus_tag="LGK42_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBK2660827.1"
                     /translation="YGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQSRMDA
                     TRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDA
                     RKSRLSGGRMTKETQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAE
                     NSRIMGILNCEEAHGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQ
                     GAPAP"
BASE COUNT          156 a          190 c          214 g          110 t
ORIGIN      
        1 attacggtgc tgcgctggag aaacagggtg tggaaatcac gctgatttac agcggcagcc
       61 ataaggtgga tggcaacccc tacagccatc ttccggatga cgtccgggag acactgcagt
      121 cccggatgga cgcaacccgc cagatgtttg cgcagaaggt gtcggcatat accggcctgt
      181 ccgtgcaggc tgtgctggat accgaggctg cagtgtacag cggtcaggag gccattgatg
      241 ccggactggc tgatgaactt gttaacagca ccgatgcgat caccgtcatg cgtgatgcac
      301 tggatgcacg taaatcccgt ctctcaggag ggcgaatgac caaagagact caatcaacaa
      361 ctgtttcagc cactgcttcg caggctgacg ttactgacgt ggtgccagcg acggagggcg
      421 aaaacgccag cgcggcgcag ccggacgtga acgcgcagat caccgcagcg gttgcggcag
      481 aaaacagccg cattatgggg atcctcaact gtgaggaggc tcacggacgc gaagaacagg
      541 cacgcgtgct ggcagaaacc cccggtatga ccgtggaaac ggcccgccgc attctggccg
      601 cagcaccaca gagtgcacag gcgcgcagtg acactgcgct ggatcgtctg atgcaggggg
      661 caccggcacc
//
LOCUS       DAFAVF010000393          660 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.446, whole genome shotgun sequence.
ACCESSION   DAFAVF010000393 DAFAVF010000000
VERSION     DAFAVF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.446"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>660
                     /locus_tag="LGK42_004702"
     CDS             <1..>660
                     /locus_tag="LGK42_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBK2660828.1"
                     /translation="YGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCI
                     PDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQL
                     QSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPY
                     ATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRYGRLTEKTDLIPEGGIRT
                     D"
BASE COUNT          150 a          224 c          189 g           97 t
ORIGIN      
        1 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc
       61 agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg
      121 cgaaccgctg tataccggac agcctgcccg ccgtggaatg gctgacctac ggcagcggtt
      181 acctggcagg catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc
      241 accgggaaac gctgcgcagc ttcggccgtt atgaactcac caccgcttat acccctgccg
      301 ggcagttaca gagccagcac ctgaacagcc tgctgtctga ccgcgattac acctggaacg
      361 acaacggcga actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagca
      421 ccaccggcag gctgaccggc gttcacacca ccgcagcgaa ccttgatatc cgcatcccgt
      481 atgccacaga cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc
      541 tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac cggtatgacc
      601 gttacggcag gctgacggag aaaaccgacc tcatcccgga agggggtatc cgtacggatg
//
LOCUS       DAFAVF010000394          612 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.455, whole genome shotgun sequence.
ACCESSION   DAFAVF010000394 DAFAVF010000000
VERSION     DAFAVF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.455"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..518)
                     /gene="fimE"
                     /locus_tag="LGK42_004703"
     CDS             complement(<1..518)
                     /gene="fimE"
                     /locus_tag="LGK42_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBK2660829.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTA"
BASE COUNT          180 a          158 c          130 g          144 t
ORIGIN      
        1 gcggtataac gcacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacgg
       61 gtatctgcac cacgctccgc cagttcataa ccgcaagcat gccttaacat atgaggatga
      121 gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg
      181 cgagaaagcc gactcccgcg gcgagaaata aatatagcgt cggtccggtc agcgcctttc
      241 cagttagcac gttcctgggt ccagcgttcc acggcttcac gctcatcaaa acgtaacggg
      301 tgaacggtag aaaatccgtt cttcagtcgg cgaatattta ttcgaccttc attaaggtca
      361 aggtcctgat aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac
      421 agaataagac aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc
      481 tgaacttctt taccggtaag ataacgacgt ttactcacaa tagtagtact cctgactgag
      541 atatatttga atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa
      601 aaagataacc ac
//
LOCUS       DAFAVF010000395          600 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.457, whole genome shotgun sequence.
ACCESSION   DAFAVF010000395 DAFAVF010000000
VERSION     DAFAVF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.457"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>600
                     /locus_tag="LGK42_004704"
     rRNA            <1..>600
                     /locus_tag="LGK42_004704"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          151 a          134 c          197 g          118 t
ORIGIN      
        1 caagcctgat gcagccatgc cgcgtgtatg aagaaggcct tcgggttgta aagtactttc
       61 agcggggagg aagggagtaa agttaatacc tttgctcatt gacgttaccc gcagaagaag
      121 caccggctaa ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa
      181 ttactgggcg taaagcgcac gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc
      241 aacctgggaa ctgcatctga tactggcaag cttgagtctc gtagaggggg gtagaattcc
      301 aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc ggtggcgaag gcggccccct
      361 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
      421 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      481 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      541 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAFAVF010000396          581 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.465, whole genome shotgun sequence.
ACCESSION   DAFAVF010000396 DAFAVF010000000
VERSION     DAFAVF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.465"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>581
                     /locus_tag="LGK42_004705"
     rRNA            <1..>581
                     /locus_tag="LGK42_004705"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAFAVF010000397          581 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.466, whole genome shotgun sequence.
ACCESSION   DAFAVF010000397 DAFAVF010000000
VERSION     DAFAVF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.466"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(87..>581)
                     /gene="hdeD"
                     /locus_tag="LGK42_004706"
     CDS             complement(87..>581)
                     /gene="hdeD"
                     /locus_tag="LGK42_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBK2660830.1"
                     /translation="IAVLLFIVGLLCISFPFVSGDILSTVVGALLICSGIALIVGLFS
                     NRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAFIAGLFCVAGVIRLMSWYR
                     QRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLVGIELIFSAASLFSFASLF
                     VKQQ"
BASE COUNT          197 a          149 c          133 g          102 t
ORIGIN      
        1 tgatctacaa cgctgcgtag cggagagagt attaatcgga tcatagtcac atcaagtgac
       61 tatgatccgg gtgacaaccg ggataattat tgctgcttaa cgaacaaact ggcgaagctg
      121 aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac cagcgttaca
      181 gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag cacaccaata
      241 acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat caggcgaata
      301 acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat gcccagctcc
      361 ggtgcgcgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag gaaaccggat
      421 aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag cgcaatgccc
      481 gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac gaacgggaaa
      541 ctgatacaca gcaacccgac gataaacaac agcacggcaa t
//
LOCUS       DAFAVF010000398          574 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.470, whole genome shotgun sequence.
ACCESSION   DAFAVF010000398 DAFAVF010000000
VERSION     DAFAVF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.470"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            36..>574
                     /locus_tag="LGK42_004707"
     CDS             36..>574
                     /locus_tag="LGK42_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660831.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVT"
BASE COUNT          121 a          139 c          144 g          170 t
ORIGIN      
        1 attaacaata acatgatatt catcacggag tgactatgtt acagatagtc ggcgcgctga
       61 tcctgctgat cgcaggattt gccattcttc gccttttgtt cagagcatta atcagcacgg
      121 cttctgcgct ggcagggctc atattgctgt gtctgttcgg cccggcctta ctggctggct
      181 atatcaccga acgcataacc cggttgttcc atattcgctg gctggcaggc gtatttctga
      241 cgattgccgg aatgatcatc agcttcatgt ggggacttga tggtaaacat atcgcgctgg
      301 aggctcacac ctttgactct gtgaaattta ttctgaccac cgctctcgcc ggtggtctgc
      361 tggctgttcc cctgcagatc aaaaacattc agcagaacgg gatcacacca gaagatatca
      421 gcaaggaaat taacgggtat tactgctgtt tttatactgc ctttttcctt atggcgtgtt
      481 ctgcatgcgc accattgatc gcgttacagt acgatatttc accgtcactg atgtggtggg
      541 gcgggttgtt gtactggctg gctgcattag tgac
//
LOCUS       DAFAVF010000399          563 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.472, whole genome shotgun sequence.
ACCESSION   DAFAVF010000399 DAFAVF010000000
VERSION     DAFAVF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.472"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..228
                     /locus_tag="LGK42_004708"
     CDS             <1..228
                     /locus_tag="LGK42_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660832.1"
                     /translation="PRVRKNYILQCGPGRFRNRKKLNALLNILESQFRLSVVPEGKTM
                     YVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          141 a          134 c          143 g          145 t
ORIGIN      
        1 ccccgtgtca ggaaaaatta tattctccag tgtggcccgg gccggttcag aaacaggaaa
       61 aaactgaacg cattgctgaa cattcttgaa tctcagttca ggctttctgt cgtgccggaa
      121 ggaaaaacca tgtatgttct gcttccccag atagccagtt taaagttgtc tgatgtctct
      181 ggtattttta cctcgggtta tcactacaat aagctgagag cgaagtaacc ggtgagcagt
      241 ttttctccat tatgcttcag acttagatga ttgattatta tggggatttc atcaccgata
      301 ttcagatgta tgaatatcgg tgatgaacta aagctcagga gctgcgtggt ggtgatgaat
      361 caacaggcat ataactgata cccgccggtc agaagttcca gcctgcgact ttcctgtttg
      421 cgtgcccgca gtccggcgtt caggtgcagc gtgtgacaga acgcacggag agcgaaacgg
      481 caggcagaca ggccgttcag tcagccctgg caccctgccc ggataccagc ttccctgaaa
      541 caactggcat caggtgcgct tat
//
LOCUS       DAFAVF010000400          545 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.477, whole genome shotgun sequence.
ACCESSION   DAFAVF010000400 DAFAVF010000000
VERSION     DAFAVF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.477"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..489
                     /locus_tag="LGK42_004709"
     CDS             <1..489
                     /locus_tag="LGK42_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBK2660833.1"
                     /translation="AYIPPHTGLPANSTDIAPPDIPAGFVAVFNSDESSWHLVEDHRG
                     KTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWNGTAWVKDTEAEKLFRIREAEE
                     IKNNLMQVASEHIAPLQDAADLEIATEEEISLLEAWKKYRVLLNRVDTSTSQDIEWPA
                     LP"
BASE COUNT          154 a          111 c          148 g          132 t
ORIGIN      
        1 gcatatattc cgcctcatac aggtctgcca gcaaacagta ccgatattgc accgccagat
       61 attccggcag gcttcgtggc tgttttcaac agtgatgagt catcgtggca tctcgttgaa
      121 gatcatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt tatttctgaa
      181 ctcggtccgt taccggaaaa tgttacctgg ttatcgccgg aaggagagtt tcagaagtgg
      241 aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat ccgggaggcg
      301 gaagaaataa aaaacaacct gatgcaggta gccagtgagc atattgcgcc gcttcaggat
      361 gctgcagatc tggaaattgc aacggaggaa gaaatctcgt tgctggaagc atggaaaaag
      421 tatcgggtat tgctgaaccg tgttgatacg tcaacttcac aggatattga atggccagca
      481 ctgccgtagg gtaaaacata taaattctat aattagatgt atctttccat ttacggcaag
      541 gaagg
//
LOCUS       DAFAVF010000401          534 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.481, whole genome shotgun sequence.
ACCESSION   DAFAVF010000401 DAFAVF010000000
VERSION     DAFAVF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.481"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            87..335
                     /locus_tag="LGK42_004710"
     CDS             87..335
                     /locus_tag="LGK42_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660834.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(332..>534)
                     /locus_tag="LGK42_004711"
     CDS             complement(332..>534)
                     /locus_tag="LGK42_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660835.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          119 c          126 g          153 t
ORIGIN      
        1 agacttctgg ttcgctcaat ggttcttaca caaacttagg ttcacataga ggttcattct
       61 cagggcggaa ttcagggggc agtacattgt ttatttatgc atctggaggt aatggaggat
      121 ctgccggagg tgcatgtgca aatacatccc gactgcaggg atatgttggt gggacgctaa
      181 ttagcgtgaa tgccagcaat aaccctgcat atggaaaaac agcctttatc agctttgctg
      241 tacctgcagg tacttcctat cagataacat cctatccaac agaaaataca tcatgtggtg
      301 ccggggtatt ttcagtattt ggatatcaaa cttaattgag cgttacacac gacgcaatac
      361 aacctgtctt atgctcgtat ttgtaccatt tgcgtgaacc tgctgggctc tcaactacct
      421 ggcagtagtg ggcatcctct gcgttgccca ttctggcaat ggcacagaag tcgaattgcc
      481 ccagataacc tgtggtggaa agctgagtaa ctttgagttt tccacctatt gtcc
//
LOCUS       DAFAVF010000402          533 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.482, whole genome shotgun sequence.
ACCESSION   DAFAVF010000402 DAFAVF010000000
VERSION     DAFAVF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.482"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..>533)
                     /locus_tag="LGK42_004712"
     CDS             complement(<1..>533)
                     /locus_tag="LGK42_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBK2660836.1"
                     /translation="TGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSF
                     TYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLD
                     RREVLHTQGEAGLKRVV"
BASE COUNT           83 a          175 c          162 g          113 t
ORIGIN      
        1 caccacccgc ttcagcccgg cttcgccctg cgtgtgcagc acttcacggc ggtccaggct
       61 gtcggtgatg gtgatgcggt ctttctcata ctgatacgtg tagcttaagc ctgccgggtt
      121 tagctgttct gtcacccgcc cgtcgctgtc gtaacggtaa cggatttccg gtcggcccgt
      181 gtgacggtgc gccaccatcc ggccccggta tttatcatcg taagtaaagc tgcgcacctg
      241 tgtattgctg cggtcataca ctgccgccag ttcgccgcgc ggcgtccagc catagcgcac
      301 cagcggcgcg gcaggtaaat tctccgggta ttccgggtcg tgcgtcagcc acacggcaga
      361 cagacggatg ccgttgtccc ggccatattc ggtgtaaccc ggcagggtat caggaaaagc
      421 ggacggttcc gtcccgccgg aaatggcctg ctgccgggct tcttctgccc gctgcgcctg
      481 cgtggtcagt accagccgga agtgacgccc ggcaccatcc gtcacgccgg tga
//
LOCUS       DAFAVF010000403          527 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.484, whole genome shotgun sequence.
ACCESSION   DAFAVF010000403 DAFAVF010000000
VERSION     DAFAVF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.484"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..>527
                     /gene="tuf"
                     /locus_tag="LGK42_004713"
     CDS             <1..>527
                     /gene="tuf"
                     /locus_tag="LGK42_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK2660837.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGR"
BASE COUNT          128 a          130 c          146 g          123 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccg
//
LOCUS       DAFAVF010000404          526 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.485, whole genome shotgun sequence.
ACCESSION   DAFAVF010000404 DAFAVF010000000
VERSION     DAFAVF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.485"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            37..>526
                     /locus_tag="LGK42_004714"
     CDS             37..>526
                     /locus_tag="LGK42_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK2660838.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSV"
BASE COUNT          162 a          124 c          100 g          140 t
ORIGIN      
        1 atataccatg gaagaaaaat ataaccggag tagtgtatgg ccaatctcta cgacttgaaa
       61 aagttcgact taaaccttct tgtcatattt gagtgtattt accagcattt aagcattagc
      121 aaagctgctg aatcactata tatcacccca tcagccgtga gccagtcgtt acaacgctta
      181 cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc gccgacgaca
      241 acagggttga atctgcatca ccatcttgag aaaaacctca aaggccttga acaaacaatt
      301 aatatagtca ataagtccga actaaagaaa aactttatta tttacggccc tcagctcatt
      361 tcttgctcta ataatagtat gctgatccgc tgcctacggc aagatgcctc tgtcgagatt
      421 gagtgccatg atatcctcat gtcagcagag aatgctgaag aactgttggt tcaccgcaaa
      481 gccgatctgg tgatcaccca aatgccagtg atcagccgtt ccgtca
//
LOCUS       DAFAVF010000405          445 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.509, whole genome shotgun sequence.
ACCESSION   DAFAVF010000405 DAFAVF010000000
VERSION     DAFAVF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.509"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..>445)
                     /locus_tag="LGK42_004715"
     rRNA            complement(<1..>445)
                     /locus_tag="LGK42_004715"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          140 c           98 g          124 t
ORIGIN      
        1 cgaagttacg gcaccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta
       61 ttctctacct gaccacctgt gtcggtttgg ggtacgattt gatgttacct gatgcttaga
      121 ggcttttcct ggaagcaggg catttgttgc ttcagcaccg tagtgcctcg tcatcacgcc
      181 tcagccttga ttttccggat ttgcctggaa aaccagccta cacgcttaaa ccgggacaac
      241 cgtcgcccgg ccaacatagc cttctccgtc cccccttcgc agtaacacca agtacaggaa
      301 tattaacctg tttcccatcg actacgcctt tcggcctcgc cttaggggtc gactcaccct
      361 gccccgatta acgttggaca ggaacccttg gtcttccggc gagcgggctt ttcacccgct
      421 ttatcgttac ttatgtcagc attcg
//
LOCUS       DAFAVF010000406          428 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.513, whole genome shotgun sequence.
ACCESSION   DAFAVF010000406 DAFAVF010000000
VERSION     DAFAVF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.513"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            353..>428
                     /locus_tag="LGK42_004716"
     CDS             353..>428
                     /locus_tag="LGK42_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBK2660839.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITA"
BASE COUNT          124 a           97 c          105 g          102 t
ORIGIN      
        1 tttttgtctt tatctcttcc acaagtaaat attggcaacc cgagctggaa gcatcatcca
       61 gagtactcat gaaacgactg taacagctgt tgaaaaaccg gaatgcactc ttcattgcaa
      121 acatttcagg gatgaagtga agatgaattg agtcgtagta tccacaccaa gccggaatta
      181 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
      241 aaaacccccg cacgttgaga ggtaaaacct gacaacagaa cgggggctaa tcttgaacca
      301 tcaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
      361 cgcgaagttt gccatgattt tctggcacga cctggcagca ccgatcctgg cggggattat
      421 taccgcag
//
LOCUS       DAFAVF010000407          424 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.514, whole genome shotgun sequence.
ACCESSION   DAFAVF010000407 DAFAVF010000000
VERSION     DAFAVF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.514"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(32..>424)
                     /locus_tag="LGK42_004717"
     CDS             complement(32..>424)
                     /locus_tag="LGK42_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660840.1"
                     /translation="KSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQYDTE
                     LPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQCHHD
                     YYGEQYCTHCQTGGFSIPRTTQEEICEF"
BASE COUNT           88 a          105 c          117 g          114 t
ORIGIN      
        1 tggcctttgc tgtcattcgc catataacag ttcagaattc gcatatttcc tcctgagttg
       61 tccggggaat actgaatcct ccggtctggc agtgtgtgca gtactgctcc ccataataat
      121 cgtggtgaca ttgtctgcag tgccagctgg ctttacgcac cacgggtaag gcatccggta
      181 cgaattgctg cagacgttta atcaactgta tttctctgcg ctccggcctg acataagggc
      241 actgttgacc gtgctctgtc agcctgtcgt cagtgtgttc aaaccaggga agttcagtgt
      301 cgtattgcgg atggtatctg agtgcactgc cacacaagtg gcaggtgtag tgagcataag
      361 gtgcggcctg tgcagtacgg gcgcccgtca gacgtccgtt gccatcaaga gcgataaagg
      421 attt
//
LOCUS       DAFAVF010000408          412 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.denovo.515, whole genome shotgun sequence.
ACCESSION   DAFAVF010000408 DAFAVF010000000
VERSION     DAFAVF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.denovo.515"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            58..134
                     /locus_tag="LGK42_004718"
     tRNA            58..134
                     /locus_tag="LGK42_004718"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Ile,seq:gat)
     gene            177..252
                     /locus_tag="LGK42_004719"
     tRNA            177..252
                     /locus_tag="LGK42_004719"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:210..212,aa:Ala,seq:tgc)
BASE COUNT          113 a           87 c          102 g          110 t
ORIGIN      
        1 tgtgctttgc agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg
       61 cttgtagctc aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc
      121 actcaggcct accaaatttg cacggcaaat ttgaagaggt tttaactaca tgttatgggg
      181 ctatagctca gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc
      241 atagctccac catctctgta gtgattaaat aaaaaatact tcagagtgta cctgcagagg
      301 ttcactgcga agttttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac
      361 aacgagagtt gttcgtgagt ctctcaaatt ttcgcaacac gatgatgaat cg
//
LOCUS       DAFAVF010000409         9829 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.155, whole genome shotgun sequence.
ACCESSION   DAFAVF010000409 DAFAVF010000000
VERSION     DAFAVF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.155"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            117..1256
                     /locus_tag="LGK42_004720"
     CDS             117..1256
                     /locus_tag="LGK42_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBK2660841.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1253..1867
                     /locus_tag="LGK42_004721"
     CDS             1253..1867
                     /locus_tag="LGK42_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBK2660842.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1924..3381)
                     /gene="pepD"
                     /locus_tag="LGK42_004722"
     CDS             complement(1924..3381)
                     /gene="pepD"
                     /locus_tag="LGK42_004722"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBK2660843.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3642..4100
                     /gene="gpt"
                     /locus_tag="LGK42_004723"
     CDS             3642..4100
                     /gene="gpt"
                     /locus_tag="LGK42_004723"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK2660844.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4192..5436
                     /gene="frsA"
                     /locus_tag="LGK42_004724"
     CDS             4192..5436
                     /gene="frsA"
                     /locus_tag="LGK42_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBK2660845.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5494..5895
                     /gene="crl"
                     /locus_tag="LGK42_004725"
     CDS             5494..5895
                     /gene="crl"
                     /locus_tag="LGK42_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBK2660846.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5934..6989)
                     /gene="phoE"
                     /locus_tag="LGK42_004726"
     CDS             complement(5934..6989)
                     /gene="phoE"
                     /locus_tag="LGK42_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBK2660847.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            7277..8380
                     /gene="proB"
                     /locus_tag="LGK42_004727"
     CDS             7277..8380
                     /gene="proB"
                     /locus_tag="LGK42_004727"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK2660848.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8392..9645
                     /gene="proA"
                     /locus_tag="LGK42_004728"
     CDS             8392..9645
                     /gene="proA"
                     /locus_tag="LGK42_004728"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK2660849.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         2369 a         2416 c         2604 g         2440 t
ORIGIN      
        1 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
       61 ttcaagtcgc aacacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
      121 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
      181 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
      241 tagtggggat gccagattta catcccggac gcggctatcc gattggcgca gcgttcttct
      301 ctgttggtcg tttttatccg gcactggtcg gcaatgatat cggctgcggt atggcgctat
      361 ggcaaacaga tattctcact cgcaaataca acgccgataa gtttgaaaag cgattatcag
      421 atctggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
      481 agcatccgtg gcgcagctcg cttggctcta tcggtggcgg caatcatttc gctgaactgc
      541 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcacaac
      601 atctgcaact gttggttcat agcggctcgc ggggggtggg gcagtctatt ttacagcggc
      661 atattgcctc gttctcgcat catggtttgc ctcaaggcag tgacgacgcg ctacggtata
      721 ttgcggaaca tgacgatgcg ctggcgtttg cgtgtattaa tcgccagatg atcgctttgc
      781 gcataatgca acaagttaag gccacgggaa gtccggttct ggatgtggcg cataactttg
      841 ttagcgcgtg tcaaatcggc gatcaacagg gctggttgca tcgtaaaggt gccacgccgg
      901 atgacaacgg tctggtgatt attcccggtt cacgtggtga ttactcctgg attgtacagc
      961 ctgttgcgaa cgaaaaaacg ctccactcgc tggcgcatgg ggcagggcgt aaatgggggc
     1021 gcaccgagtg taaagggcgt ctggcagcga aatacacggc gacgcaactc tcgcgtactg
     1081 aacttggcag tcgggtaatt tgtcgcgata aacaactcat ctttgaagaa gcgccacaag
     1141 cctataaatc ggctgaaagc gtggtgcaat gtctggtgca ggccggttta attattcctg
     1201 tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa ggatgatctt
     1261 gctacaactc tcctcagcgc aggggccgga agaatgttgt ctcgcagtga aaaaggcgct
     1321 ggacaggcta attaaagaag ctgcccgaca agacgtcgcg gtaacggtgt tggaaacaga
     1381 aacgggccgc tactctgaca cgctgcgttc agctctggtt tctctggatg gcgataacgc
     1441 atgggcatta agcgaaagct ggtgcggcac tattcagtgg atttgtccga gtccgtatcg
     1501 gcctcatcat gggcgcaaaa actggtttct gggcattggg cgttttaccg ctgatgagca
     1561 ggaacaatcg gatgcaatcc gttatgagac gttgcgttcg tcggggccgg gcggtcaaca
     1621 tgtcaataaa accgactcgg cggtacgcgc cacgcatttg gcatccggta ttagcgtgaa
     1681 ggttcagtca gagcgtagtc agcatgctaa caagcggctg gcacgattgc tgattgcctg
     1741 gaagctggag cagcagcaac aggaaaatag cgcggtgctg aaatcgcagc ggcgaatgtt
     1801 ccatcaccag attgaacgtg gcaacccgag acggacattt acagggatgg cttttatcga
     1861 aggataatga aggatgaaac tgccggaagg cgattaaacg ccatccggca gcaaatcaaa
     1921 taattacttc gccggaattt ctttcagcag ttcagtcagc agtgtccagt aatgacctac
     1981 gctttcgatg tgaacttgct catccggaga gtgtggaccg gtgatagttg gcccgataga
     2041 aaccatgtcc atttccggat acggtttttt gaacagacca cattccaggc ccgcgtggat
     2101 aatctggatg ttcggtgttt tgttgaacag acgctgatag gtttcacgta ccagatgcat
     2161 caccggagaa ttagcgtccg gctgccagcc aggatatgcg cctttcgctt cggttttcgc
     2221 gccagctagt ttacccagcg aatccagcat gctcaccacg tagtctttac cgctgtcgat
     2281 cagtgaacgg atcaggcagt gaatttctac gttattgtca gtcatggtca ccacaccgac
     2341 gttcagggag gtttcgacca caccttttgc cacgtcggaa ttgcggatca caccgttcgg
     2401 ggtggcgttc agcagacgaa taaaggtatc gcgagatttc gcaatcaggg cagctttatc
     2461 gttcgctaca gagtccagca acaaggccag atttttctct ttctctgcca gctcgttttt
     2521 caggatctcc tgataggtat tcaccagaga tttcaggacg tcgactttat cagctgcgac
     2581 agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc cgttgaaatc
     2641 gataaggcgc agatccagtt cttccgcatg acccgccagg aagcgcacca gtagtttgtt
     2701 ggcattaccc aggccaacgt ggatttcacc gccggagtga ccgcctttca gaccttttaa
     2761 ggttaacttg aaggtttcaa aaccagctgg aaccgcttca cgatctaaat gcaggttgga
     2821 ggtgaagtcg ataccccccg cacaacccat gtagatttca ccttcttctt cggagtcggt
     2881 gttaatcaga atatcagcct gcaaccagtt gctctgtaag ccgaacgcac cgtccatacc
     2941 ggcttcttcg gtcatggtca gcagcacttc cagcgggccg tgaaccacgt tttcgtcagc
     3001 cagaaccgcc agcgcagacg ccataccaat gccgttatct gcacccagcg tggtgccgcg
     3061 cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga agtcatgcac
     3121 ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga ccggtttacg
     3181 attttccata cctgcggtag caggtttacg aatcaggata ttacctacct gatcgcgttc
     3241 gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg
     3301 ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg gctgtggaga
     3361 taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc
     3421 aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtttga
     3481 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     3541 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     3601 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     3661 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     3721 aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg
     3781 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
     3841 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
     3901 ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag
     3961 cgcactttgt caccatcttt gcaaaaccgg ctggtcgtcc gctggttgat gattatgttg
     4021 ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc
     4081 cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta
     4141 acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag
     4201 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
     4261 gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc
     4321 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg tattgaccca
     4381 cgtgaaatcc tagacgtcca ggcacgtatt gtgatgagcg atgctgaacg taccgacgat
     4441 gatttatacg atactgtgat tggctaccgt ggtggcaact ggatttatga gtgggccacc
     4501 caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag atccgcaact cagtggtcga
     4561 cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct gaaaggagat
     4621 gacctggccg agcaagcgca ggctttatca aaccgcgcct atgaagaggc cgcccagcgt
     4681 ctgccgggca cgatgcggca gatggagttt actgtacccg gcggtgcgcc catcaccggc
     4741 tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg tggtggtctg
     4801 gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt
     4861 gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa gctcacccag
     4921 gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc gtgggtggat
     4981 cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt gcgtctggca
     5041 taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt tcataccctg
     5101 ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt tctggcgagt
     5161 cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa tcgctattca
     5221 ttaaaagtgc aaggattgct gggacgtcgc tgcccaacgc caatgttatc aggctactgg
     5281 aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc atctgctgac
     5341 ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa aggtcttcag
     5401 gaaatcaccg gctggatcga aaaacgcttg tgttaaaaat ttgctaaatt ttgccaattt
     5461 ggtaaaacag ttgcatcaca acaggagata gcaatgacgt taccgagtgg acacccgaag
     5521 agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg taagtgcgaa
     5581 gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc ggcaccggaa
     5641 gtgcgtgaat tctggggctg gtggatggag ctggaagcgc aggaatcccg ttttacctac
     5701 agctaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc ggtaaaagat
     5761 actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt ttcacgagaa gctgcgtgaa
     5821 ctgctgacga cgctgaatct gaagctggaa ccggcggatg attttcgtga cgagccggtg
     5881 aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat ctattaaaac
     5941 tgatacgtca tcccaatcgc gacaatatca tcattattaa tattcaattt gttatcgcta
     6001 tccagttggt tgattttata atcaacaaat gctgacatat ttttgttgaa ataatacgta
     6061 gcaccgacgt cgatataatt aaccagatct tcaccaccga taccttcaat atctttccct
     6121 ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta ttgagcgacc
     6181 gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat tttgcgtgtt
     6241 tcagaataaa aagttgccag ataaatatta ttggcatcgt atttcagacc tgtagcccat
     6301 gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt gcgatctgag
     6361 ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt caatgacgtg
     6421 ccgaagccat cgccgttttg ctttttaacg tcgcggtttt cgtttttccc ttgatattgc
     6481 agggttaagt tcaggccatc gataacgccg aagaagtcgg tgttccgata cgtcgccaga
     6541 ccgctggcgc gtttggtcat aaagttgtcg gtctgcgccg aggagtcgcc accaaattcc
     6601 gggaacatat cggtccaggc ttccacgtca tacaacgcgc ccaggttacg gccatagtcg
     6661 aaagaaccca aatctttata cttcaatccg gcaaaagcga gacgcgtttt ttgctgtgca
     6721 gtatcactct ccgctttatt tccggcaaac tccgcttccc aacggccata gccagtcagt
     6781 tgatcgttaa tttgtgtttc gcctttaaaa ccaaaacgga tataactctg gtcgccatct
     6841 ttactgtcgt tatcactcat ataatgcatg gctttaactt tgccatagac atccagttta
     6901 ttaccgtctt tgttatatat ttctgcggcc tgtacggatg cagatgccac aatgcccatc
     6961 accactaatg ccagagtgct ctttttcatt ttcattcctg attttaatta acgcgcgaat
     7021 attcagcggg agagccccgt tgaaaacagg aaagttttta acctgagatt gttaaagata
     7081 tattacagat taataatatt cttaaaatgt ggtaatttat taaatctgta ataaaagcgt
     7141 aaacaactgc cgctaggctt gctgatcccg cgcaacaaaa cgccatgctt tgcttgcaga
     7201 tggttggcaa ccgacgacag tcctgctaaa acgttcgttt gatatcattt ttcctaaaat
     7261 tgaatggcag agaatcatga gtgacagcca gacgctggtg gtaaaactcg gcaccagtgt
     7321 gctaacaggc ggatcgcgcc gtctgaaccg tgcccatatc gttgaacttg ttcgccagtg
     7381 cgcgcagtta catgccgccg ggcatcggat tgttattgtg acgtcgggcg cgatcgccgc
     7441 cggacgtgag cacctggggt acccggaact gccagcgact atcgcctcga aacaactgct
     7501 ggcggcggta gggcagagtc gactgattca actgtgggaa cagctgtttt cgatttatgg
     7561 cattcacgtc gggcagatgc tgctgacccg tgctgatatg gaagaccgtg aacgcttcct
     7621 gaacgcccgc gacaccctgc gtgcgttgct cgataacaat atcgttccgg taatcaatga
     7681 gaacgatgct gtcgctacgg cagagattaa ggtcggcgat aacgataacc tttctgcgct
     7741 ggcggcgatt ctggcgggtg ccgataaact gttgctgctg accgatcaaa aaggtttgta
     7801 taccgctgat ccgcgcagca atccgcaggc agaactgatt aaagatgttt acggcattga
     7861 tgacgcactg cgcgcgattg ccggtgacag cgtttcaggc ctcggaactg gcggcatgag
     7921 taccaaattg caggccgctg acgtggcttg ccgtgcgggt atcgacacca ttattgccgc
     7981 gggcagcaag ccgggcgtta ttggtgatgt gatggaaggc atttccgtcg gtacgctgtt
     8041 ccatgcccag gcgactccgc ttgaaaaccg taaacgctgg attttcggtg cgccgccggc
     8101 gggtgaaatc acggtagatg aaggggcaac tgccgccatt cttgaacgcg gcagctccct
     8161 gttgccgaaa ggcattaaaa gcgtgactgg caatttctcg cgtggtgaag tcatccgcat
     8221 ttgcaacctc gaaggtcgcg atatcgccca cggcgtcagt cgttacaaca gcgatgcatt
     8281 acgccgtatt gccgggcacc actcgcaaga aattgatgca atactgggat atgaatacgg
     8341 cccggttgcc gttcaccgtg atgacatgat tacccgttaa ggagcaggct gatgctggaa
     8401 caaatgggca ttgccgcgaa gcaagcctcg tataaattag cgcaactctc cagccgcgaa
     8461 aaaaatcgcg tgctggaaaa aatcgccgat gaactggaag cacaaagcga aatcatcctc
     8521 aacgctaacg cccaggatgt tgctgacgcg cgtgccaatg gccttggcga agcgatgctt
     8581 gaccgtctgg cactgacgcc cgcacggctg aaaggcattg ccgatgatgt gcgccaggtg
     8641 tgtaacctcg ccgatccggt ggggcaggta atcgatggca gcgtactgga cagcggcctg
     8701 cgtcttgagc gtcgtcgcgt accgctgggg gttattggcg tgatttatga agcgcgcccg
     8761 aacgtgacgg ttgatgtcgc ttcgctgtgc ctgaaaaccg gtaatgcggt gatcctgcgc
     8821 ggtggcaaag aaacgtgtcg cactaacgct gcaacggtgg cggtgattca ggacgccctg
     8881 aaatcctgcg gcttaccggc gggtgccgtg caggcgattg ataatcctga ccgtgcgctg
     8941 gtcagtgaaa tgctgcgtat ggataaatac atcgacatgc tgatcccgcg tggtggcgct
     9001 ggtttgcata aactgtgccg tgaacagtcg acaatcccgg tgatcacagg tggtataggc
     9061 gtatgccata tttacgttga tgaaagtgta gagatcgctg aagcattaaa agtgatcgtc
     9121 aacgcgaaaa ctcagcgtcc gagcacatgt aatacggttg aaacgttgct ggtgaataaa
     9181 aacatcgccg atagcttcct gcccgcatta agcaaacaaa tggcggaaag cggcgtgaca
     9241 ttacacgcag atgcagctgc actggcgcag ttgcaggcag gccctgcgaa ggtggttgct
     9301 gttaaagccg aagagtatga cgatgagttt ctgtcattag atttgaacgt caaaatcgtc
     9361 agcgatcttg acgatgccat cgcccatatt cgtgaacacg gcacacaaca ctccgatgcg
     9421 atcctgaccc gcgatatgcg caacgcccag cgttttgtta acgaagtgga ttcgtccgct
     9481 gtttacgtta acgcctctac gcgttttacc gacggcggcc agtttggtct gggtgcggaa
     9541 gtggcggtaa gcacacaaaa actccacgcg cgtggcccaa tggggctgga agcactgacc
     9601 acttacaagt ggatcggcat tggtgattac accattcgtg cgtaaataaa accgggtgat
     9661 gcaaaagtag ccatttgatt cacaaggcca ttgacgcatc gcccggttag ttttaacctt
     9721 gtccaccgtg attcacgttc gtgaacatgt cctttcaggg ccgatatagc tcagttggta
     9781 gagcagcgca ttcgtaatgc gaaggtcgta ggttcgactc ctattatcg
//
LOCUS       DAFAVF010000410         9667 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.157, whole genome shotgun sequence.
ACCESSION   DAFAVF010000410 DAFAVF010000000
VERSION     DAFAVF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.157"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(<1..93)
                     /locus_tag="LGK42_004729"
     CDS             complement(<1..93)
                     /locus_tag="LGK42_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBK2660850.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAY"
     gene            complement(312..1289)
                     /gene="epmA"
                     /locus_tag="LGK42_004730"
     CDS             complement(312..1289)
                     /gene="epmA"
                     /locus_tag="LGK42_004730"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBK2660851.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1614..3422
                     /gene="frdA"
                     /locus_tag="LGK42_004731"
     CDS             1614..3422
                     /gene="frdA"
                     /locus_tag="LGK42_004731"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBK2660852.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3415..4149
                     /gene="frdB"
                     /locus_tag="LGK42_004732"
     CDS             3415..4149
                     /gene="frdB"
                     /locus_tag="LGK42_004732"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBK2660853.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4160..4555
                     /gene="frdC"
                     /locus_tag="LGK42_004733"
     CDS             4160..4555
                     /gene="frdC"
                     /locus_tag="LGK42_004733"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBK2660854.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAEFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4566..4925
                     /gene="frdD"
                     /locus_tag="LGK42_004734"
     CDS             4566..4925
                     /gene="frdD"
                     /locus_tag="LGK42_004734"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBK2660855.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4988..6121
                     /locus_tag="LGK42_004735"
     CDS             4988..6121
                     /locus_tag="LGK42_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBK2660856.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6210..6743
                     /gene="blc"
                     /locus_tag="LGK42_004736"
     CDS             6210..6743
                     /gene="blc"
                     /locus_tag="LGK42_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBK2660857.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6740..7057)
                     /gene="sugE"
                     /locus_tag="LGK42_004737"
     CDS             complement(6740..7057)
                     /gene="sugE"
                     /locus_tag="LGK42_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBK2660858.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7232..7378)
                     /gene="ecnB"
                     /locus_tag="LGK42_004738"
     CDS             complement(7232..7378)
                     /gene="ecnB"
                     /locus_tag="LGK42_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBK2660859.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7489..7614)
                     /gene="ecnA"
                     /locus_tag="LGK42_004739"
     CDS             complement(7489..7614)
                     /gene="ecnA"
                     /locus_tag="LGK42_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBK2660860.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7666..8232)
                     /gene="efp"
                     /locus_tag="LGK42_004740"
     CDS             complement(7666..8232)
                     /gene="efp"
                     /locus_tag="LGK42_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK2660861.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8274..9302
                     /gene="epmB"
                     /locus_tag="LGK42_004741"
     CDS             8274..9302
                     /gene="epmB"
                     /locus_tag="LGK42_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBK2660862.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2445 a         2482 c         2461 g         2279 t
ORIGIN      
        1 ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca atatgagtcc
       61 tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt
      121 ataaaacgcg cccactgaac tgcactgccc aacattgagc gggcagcgta aaaaccaata
      181 cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct ttaacggaaa
      241 aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt taattcagta
      301 atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag tgtctccgcg
      361 cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca gtcaggcata
      421 ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa accgcgcgcc
      481 gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc cgtcaattca
      541 tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc agcgacccga
      601 tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg gtacacaaag
      661 gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa ttgtagcagc
      721 gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc tgcgacttcc
      781 cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa gaaagcttgt
      841 tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag atcgtccacc
      901 tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat agtgaactca
      961 gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc
     1021 ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga
     1081 ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata
     1141 tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca
     1201 aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg aatggatgcg
     1261 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
     1321 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
     1381 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
     1441 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
     1501 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
     1561 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
     1621 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
     1681 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
     1741 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
     1801 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
     1861 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tggggatgcc
     1921 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
     1981 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
     2041 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
     2101 ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc acgctggtgc
     2161 agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca
     2221 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     2281 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     2341 tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     2401 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     2461 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     2521 acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac ctcggcgaga
     2581 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     2641 atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg
     2701 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     2761 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     2821 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     2881 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc
     2941 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggtctggct atggaagaag
     3001 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     3061 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     3121 tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     3181 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     3241 gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     3301 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     3361 gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct
     3421 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     3481 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     3541 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     3601 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     3661 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     3721 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     3781 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     3841 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     3901 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     3961 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta
     4021 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     4081 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     4141 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     4201 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     4261 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     4321 ggaagcctgg gcggaattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     4381 gatcactctg gcggcagctc tgctgcatac caaaacctgg tttgaactgg caccgaaagc
     4441 agccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     4501 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     4561 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     4621 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     4681 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     4741 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     4801 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat
     4861 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa
     4921 tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     4981 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt
     5041 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     5101 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     5161 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     5221 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga
     5281 aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     5341 tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat tgcaggtgcc
     5401 ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     5461 ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     5521 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct tccagccact
     5581 caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg
     5641 atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg aaacttatgg
     5701 tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga
     5761 tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct actggcaaac
     5821 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacag
     5881 catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa aagcgattac
     5941 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa ccggcggatt
     6001 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc tggctaacaa
     6061 aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta
     6121 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6181 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6241 cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc gtaacagtag
     6301 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6361 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6421 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6481 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6541 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6601 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6661 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6721 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6781 ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6841 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     6901 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acagtaataa
     6961 cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc cataccactt
     7021 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga gaattggggc
     7081 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7141 ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca aaaaaacaaa
     7201 cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt gctgcaccag
     7261 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
     7321 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
     7381 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
     7441 tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
     7501 gcgagagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca
     7561 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
     7621 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
     7681 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
     7741 aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
     7801 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
     7861 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
     7921 agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
     7981 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
     8041 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
     8101 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
     8161 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
     8221 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
     8281 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
     8341 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
     8401 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
     8461 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
     8521 ttgtcgtcgc gtccggattc tccaccgacc cgctggaaga acagcacagc gtagtgcctg
     8581 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
     8641 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
     8701 actggcagac tgctcttgag tatgttgctg cgcatccgga actggacgag atgattttct
     8761 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
     8821 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
     8881 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
     8941 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
     9001 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
     9061 acgcacaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt
     9121 acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
     9181 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg
     9241 cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
     9301 aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc aggcctacat
     9361 tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc gtttacgccg
     9421 catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg gaaatttaat
     9481 ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac tctttatata
     9541 gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg gctatttatg
     9601 ttattttaac aactattact accaagccca tttatctggc ttataaatta aaattaatta
     9661 tttaatt
//
LOCUS       DAFAVF010000411         4602 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.239, whole genome shotgun sequence.
ACCESSION   DAFAVF010000411 DAFAVF010000000
VERSION     DAFAVF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.239"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..154
                     /locus_tag="LGK42_004742"
     CDS             <1..154
                     /locus_tag="LGK42_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK2660863.1"
                     /translation="ATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLV
                     SRVFEM"
     gene            207..962
                     /locus_tag="LGK42_004743"
                     /pseudo
     CDS             207..962
                     /locus_tag="LGK42_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(1144..1320)
                     /locus_tag="LGK42_004744"
     CDS             complement(1144..1320)
                     /locus_tag="LGK42_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBK2660864.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            1650..2465
                     /gene="sul2"
                     /locus_tag="LGK42_004745"
     CDS             1650..2465
                     /gene="sul2"
                     /locus_tag="LGK42_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBK2660865.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2552..2854
                     /locus_tag="LGK42_004746"
     CDS             2552..2854
                     /locus_tag="LGK42_004746"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660866.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(3030..4175)
                     /locus_tag="LGK42_004747"
                     /pseudo
     CDS             complement(3030..4175)
                     /locus_tag="LGK42_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
BASE COUNT          976 a         1279 c         1263 g         1084 t
ORIGIN      
        1 cgccacgctg ggatttaaat ccatgaagac ggcttacgcc accatcaaag gtattgaggt
       61 gatgcgtgca ctacgcaaag gccaggcctc agcattttat tatggtgatc ccctgggcga
      121 aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt tgaataagac aaaaggctgc
      181 ctcatcgcta actttgcaac agtgccgtta aaagcaacaa aaatgacttc gttgatgctg
      241 aagctatctg tgaagcagca tcacgtccat ctatgcgttt cgtgcagccc agaaccgaat
      301 ctcagcaggc aatgcgagct ctgcatcgtg tccgtgaatc cctggttcag gataaggtga
      361 aaacaactaa tcagatgcat gcttttctgc tggaatttgg tatcagcgtt ccgcgaggtg
      421 ctgccgttat tagtcgactg agtacccttc ttgaggacag tagtttgcct ctttatctca
      481 gccagttact gctgaaatta caacagcatt atcactatct tgttgagcag attaaagatc
      541 tggaatctca gttgaaacga aagttggacg aagatgaggt tggacagcgc ttgctgagta
      601 ttccctgcgt tggaacgctg actgccagta ctatttcaac tgagattggc gacgggaagc
      661 agtacgccag cagccgtgac tttgcggcgg caacagggct ggtaccccga cagtacagca
      721 cgggaggtcg gacgacattg ttagggatta gcaagcgggg caacaaaaag atccgaactt
      781 tgttggttca gtgtgccagg gtattcatac aaaaactgga acaccagtct ggcaagttgg
      841 ccgactgggt cagggagttg ttgtgtcgga aaagcaactt tgtcgtcacc tgtgctctgg
      901 caaacaagct ggccagaata gcctgggcac tgacggcgcg acagcaaact tacgaagcat
      961 aaaggcagaa atacaccagt ttaaacaatc attcatctgg ttttgcgaat actgatattg
     1021 atgatactaa cggcccaccg gcctgttgag gaacctgtaa aacggaaagg ctcattgaag
     1081 ccgtatattt tctggaggtt catcaggcgc ggaactcatc gaggcgcggg aataaaatcc
     1141 cattcagacg ccggatagat tcaagcaagc caacttgtcg tcaaaatcgg tgttgcaaaa
     1201 acgggagtga ccatagattc cgttttctga ggcgaccccc acatagagac cttcccggtc
     1261 attcactttg tagagtttgt ccctgggctt gagattgcgc agcttggtat cggtcagcat
     1321 gtaccttgtc cttcttcgtc tccgataccc actctattgt aaacaagaca tttttatctt
     1381 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     1441 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     1501 ccgctttcct ggctttgctt ccagatgtat gctattctgc tcctgcagct aatggatcac
     1561 cgcaaacagg ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat
     1621 gctcgattat tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg
     1681 tcaacataac ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca
     1741 ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat
     1801 ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg
     1861 tgctggacgc gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga
     1921 cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag
     1981 acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg
     2041 tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg
     2101 cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc
     2161 ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg
     2221 tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt
     2281 cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga
     2341 cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc
     2401 cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc
     2461 gttaactgca cattcgggat atttctctat attcgcggtt cagcaggcat gtcccctttg
     2521 agggcgaccc gacgacagga taatcgacct tatggtgcgc aaatatttcg gcacagacgg
     2581 tattcgtggc aaagccaacg aaggcgcgat gacggcggaa accgccttgc gcgtcggcat
     2641 ggcggctggc cgtgtctttc gtcgcggtga ccaccgccat cgtgtcgtga tcggcaagga
     2701 tacgcgcctg tcgggctata tgcttgaacc cgcgctcaca gccggtttca cctcgatggg
     2761 catggacgta ttcctttttg gcccgctgcc gacaacgtat aggaagaata aacgcccttt
     2821 tcacccaagt ccaacagctt tggaccgcag ttgactcttt cgacacccct gcgatgcaac
     2881 ccaatccggc tgacggggag ccagcaacgc tgaaaattta ccctcctctt tcccactagc
     2941 ggctcctttt ccgacaacca gcacggcgga tccctgccgc ggcgctgtga acgcagcatt
     3001 ttgattggta tcgttggcct tcaggctcgt cagtcaaaca gacccaggag cagctcagcc
     3061 ggtggcgccc ggctttcggg taacgccctg gtcccgctgg tttcggcttt gtgcttcagg
     3121 tgatcaagga tctgcttgat cactataggg tcttcaatgc aggcgatgac tttcatggcg
     3181 ccgccgcagc cgctgcaggt ctcgatgtcg atattgaaaa cacgcttgag ccgttgcgcc
     3241 catgtcatcg acgctcgccg ttgtgctggt gttgccggtt catcagccac cctgaccttg
     3301 ttgcccctgc cccgttttgc cggcgtgacc aacgcccggt gccgactgtt gggtgcgaac
     3361 accccgtgga agcgggttag gttgactctg ggcttcggta ccagggcggc cagccttgca
     3421 atgaaatcca atggttcgaa aatgacgtgc gtggtgccgt cccggtacgg cgtcttgagc
     3481 tggtagcgca cgttgccgcc tcgtgttaac gacagccgct tctcggatac cgccgggcgg
     3541 ctgatgtacc ggcacagccg ttcgagcttc ttgcgttcat cggccctggc cgccacgccg
     3601 gcgtgcaggc tggacccggc taccttgcca atcccgtcac cgaacggatc accactggtc
     3661 ggcagagttt gcaaagtgaa cacctttcgc cccgcctgtg aaccgacagc gatacggtaa
     3721 gtgatcgagt gccccagcag gggtgtcatc gggtcgtcat ccaccgcatc cgaggccaga
     3781 tagctgtttt cgacatcccg ttccagcagg ccttgccgtt ccagatagcg acccacccgg
     3841 tgggcgatgg tgtgcgtcag ctgggtgagc tctggggtgg tcggcgcctt gacccagcgg
     3901 aaacgcgctg agccgtggga ttgctcgaca tacacaccgt cgagaaacag catgtggaag
     3961 tgaacattca gattgagcgc cgatccaaaa cgctggatca gggtgaccgc gcccgtcttg
     4021 gccacttggt gggtatggcc cgctttcttg accaggtgcg tggcaatgac gcggtaaacg
     4081 atgcccagca cccaccccat gatctcgggc cggctggcaa acaggaaacg cagctgaaac
     4141 gggaagctca acacccactg acgcatgggt tgttcgtcct ggacggcgaa cccggcaaag
     4201 ctcctgtaac attgcttctt cctgttttgt tagcgtgact gtcacgctag cgccttcctc
     4261 tttgagtcgc gcgcggcgcc gggcctggcg aatacgggct tgttcacgct gtcggtcatt
     4321 catatttttc ctccacgtga ctgtcacgtt gaattaaact gggcattacc cctgcggggc
     4381 cgttctgccg tgctgcgcat cgagccgcta cgcgtctcgc cccttcgggc ggcggccact
     4441 aatgcctccg gcctgcggcc tccgcgcttc gcttgtgacg atatctgaat cgttatatac
     4501 tgtgtatata cgtagtaatg acgaggtgat aaatggcaca ggttaatatg agtttaagaa
     4561 tcgacgctga actgaaggat gcttttatgg ccgctgcaaa aa
//
LOCUS       DAFAVF010000412         4584 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.240, whole genome shotgun sequence.
ACCESSION   DAFAVF010000412 DAFAVF010000000
VERSION     DAFAVF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.240"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            complement(278..463)
                     /locus_tag="LGK42_004748"
     CDS             complement(278..463)
                     /locus_tag="LGK42_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK2660867.1"
                     /translation="MMDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEI
                     DPRQSISVHLPYSQTYL"
     gene            867..1109
                     /locus_tag="LGK42_004749"
     CDS             867..1109
                     /locus_tag="LGK42_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBK2660868.1"
                     /translation="MSGEPAEFARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1141..1791)
                     /gene="tetR(A)"
                     /locus_tag="LGK42_004750"
     CDS             complement(1141..1791)
                     /gene="tetR(A)"
                     /locus_tag="LGK42_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBK2660869.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1897..3096
                     /gene="tet(A)"
                     /locus_tag="LGK42_004751"
     CDS             1897..3096
                     /gene="tet(A)"
                     /locus_tag="LGK42_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBK2660870.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3128..4012)
                     /locus_tag="LGK42_004752"
     CDS             complement(3128..4012)
                     /locus_tag="LGK42_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBK2660871.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4150..4557)
                     /locus_tag="LGK42_004753"
     CDS             complement(4150..4557)
                     /locus_tag="LGK42_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK2660872.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          934 a         1368 c         1390 g          892 t
ORIGIN      
        1 gcttcattgg tcataacgtg acgttcgcca acgatctgtt tcgttcagga gcaccagatc
       61 cgtcaccgga taactggatc agcattatcc tgggggattc ggttactgtt ggcagtggcg
      121 ccattattct gtctccgtat atatgcagcg gagctgttat cggtgcgggg gcagttgtag
      181 taaaaccggt tgagaacaaa ggcatttatg caggaaaccc ggcaagactg atcagaatat
      241 tatgaaaaaa gaaaagggcc gttactggcc ctttttatca gaggtaggtc tgtgaatacg
      301 gcaggtgtac agaaatcgac tgccggggat cgatttccag ggccgcccgg tattctgcgt
      361 cagtgatatc accttttttc agtaaataac cgagagcatc gtttcccagc cctgacagta
      421 tacgtattct ggcgatccgg aaatcaaaca ggctgtccat cataccatgc tccggaaatg
      481 cgggaggagt ggccagatag gcaggtttga ccatactgct gatgatgttc cagtatcggg
      541 aaaagtcgcg ggccgggtcc gggagaagac ctgacagcag cccaatccgg atggtttcca
      601 gccagctgtc ataaaatcgc ttaacatgaa gtgaagccat gtgctcagca caccatttgc
      661 gcatagcttc tttttccgga agggaagtgt tcccggagaa tacgttcatt acccactggc
      721 tgatgatatc aaaataggga aacgcagccc cgaaaccggc ggtatccacc gggggattaa
      781 tgacggccag agaagggtcc tctgtccaga aaaagtgctc atagagctgt gttctgtcaa
      841 atccctgaat gaacgagtaa tcaggcattt caggggagcc cgcagaattc gcccgcgagc
      901 gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc
      961 aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1021 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1081 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1141 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1201 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1261 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1321 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1381 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1441 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1501 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1561 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1621 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1681 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1741 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1801 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     1861 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc
     1921 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     1981 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2041 ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2101 cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc
     2161 atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     2221 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     2281 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     2341 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     2401 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     2461 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     2521 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     2581 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     2641 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     2701 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     2761 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     2821 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     2881 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     2941 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     3001 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     3061 ctttggagcg gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc
     3121 atgcgggtca aggcgacttc cggcaagcta tacgcgccct aggagtgcgg ttggaacgtt
     3181 ggcccagcca gatactcccg atcacgagca ggacgccgat gatttgaagc gcactcagcg
     3241 tctgatccaa gaacaaccat cctagcaaca cggcggtccc cgggctgaga aagcccagta
     3301 aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac
     3361 ccgctccgat caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga
     3421 ttggcggatc aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc
     3481 aggcggtaaa ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga
     3541 acgccatgga aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt
     3601 ttggtgtcaa caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca
     3661 tcaatcgtat cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca
     3721 cagcgcctac cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc
     3781 tccagaatag cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg
     3841 ttggaatctg tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcatcg
     3901 cgaccgtcat cggtgagaag ttcggcaggt attgggtggt gacaatgtag gtgctgcccc
     3961 aaatggcagg tgctatcgct gtgaacaata aatcgggcgt gcgtaatgac atgcgattac
     4021 aagacctccg ctacggcgat gggtttttga gagttgcagg cgccgcgatg accgcaccct
     4081 gacttggact ggccctgcct cgcaccaatc cacctgcggc tatggccgcg atcatgcgag
     4141 gcccgaaaat tacacgacct gtacacgtcc gccgaacccg gaacgcaaca gagcttcgtg
     4201 ggcatcccga tccgggtcgt agcagcagtc ctggaccaaa cgcacgtcgt agtccgcatc
     4261 actagcccag gcgacgcttg aaagaacaac gccggtggtg cttatcccgg ccatgacaag
     4321 cgtgcttacg ccgcgcgtcc gaaggtcggc gtcgagcgcc gtgccataaa agacgctggc
     4381 tcgcggacat gcatagaaaa ggtcgccccg ttcgatcgca agcccttcga cgggcagacc
     4441 ggtgcgaaat cgtccgctcg ggagatacgg tgagatttgg cgattcgtcg ccggcggcgc
     4501 atgttcatac tcttcgccga gcgaaaagtt ggggaacagc acgggccggc cgcgcaaggc
     4561 gtgctggcag ccgtgcagac cctg
//
LOCUS       DAFAVF010000413         2352 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.296, whole genome shotgun sequence.
ACCESSION   DAFAVF010000413 DAFAVF010000000
VERSION     DAFAVF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.296"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            84..959
                     /locus_tag="LGK42_004754"
     CDS             84..959
                     /locus_tag="LGK42_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-1"
                     /protein_id="HBK2660873.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(1006..1338)
                     /locus_tag="LGK42_004755"
     CDS             complement(1006..1338)
                     /locus_tag="LGK42_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBK2660874.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
BASE COUNT          604 a          569 c          612 g          567 t
ORIGIN      
        1 aggttagact ataaatagaa aaaggcgttt tgacagacta ttcatgttgt tgttaattcg
       61 tctcttccag aataaggaat cccatggtta aaaaatcact gcgtcagttc acgctgatgg
      121 cgacggcaac cgtcacgctg ttgttaggaa gtgtgccgct gtatgcgcaa acggcggacg
      181 tacagcaaaa acttgccgaa ttagagcggc agtcgggagg aagactgggt gtggcattga
      241 ttaacacagc agataattcg caaatacttt atcgtgctga tgagcgcttt gcgatgtgca
      301 gcaccagtaa agtgatggcc gtggccgcgg tgctgaagaa aagtgaaagc gaaccgaatc
      361 tgttaaatca gcgagttgag atcaaaaaat ctgacttggt taactataat ccgattgcgg
      421 aaaagcacgt cgatgggacg atgtcactgg ctgagcttag cgcggccgcg ctacagtaca
      481 gcgataacgt ggcgatgaat aagctgattt ctcacgttgg cggcccggct agcgtcaccg
      541 cgttcgcccg acagctggga gacgaaacgt tccgtctcga ccgtaccgag ccgacgttaa
      601 acaccgccat tccgggcgat ccgcgtgata ccacttcacc tcgggcaatg gcgcaaactc
      661 tgcgtaatct gacgctgggt aaagcattgg gtgacagcca acgggcgcag ctggtgacat
      721 ggatgaaagg caataccacc ggtgcagcga gcattcaggc tggactgcct gcttcctggg
      781 ttgtggggga taaaaccggc agcggtgact atggcaccac caacgatatc gcggtgatct
      841 ggccaaaaga tcgtgcgccg ctgattctgg tcacttactt cacccagcct caacctaagg
      901 cagaaagccg tcgcgatgta ttagcgtcgg cggctaaaat cgtcaccaac ggtttgtaat
      961 agcggaaacg gaatggggaa actcattccg tttttgttta tcgccttaga cggcaaaagc
     1021 gctgtcgccc acctgcgctt gcgcatacca ggccataagc tccgtggttc ctggttctcc
     1081 ttccgctggc gcccagtgcg catagtcatc ggcagccacg ggttgatagc caccgtgttt
     1141 tacttcaaaa attatgccac cggtatccag cgacagcacg gcatgccagg ttcctgcggc
     1201 catctccagc accgtacagg tttcccccaa tatcgcccga tgggtgacgg taccccgatc
     1261 gtcaaaattc agcaccacga aacgacccct taatggcaac agtagttcga aggtgtgagg
     1321 gtgtcggtgc gggcgcacgt aggttcctgg taaaaacatt tacgttatat cccaaaaaca
     1381 cacaggtctt agggaggttt aaactgtgta ttaacaccta tcgtattgac gggcgtgaaa
     1441 tggcagaaac agaagtcgtt cctattgaca tgcctgactc taacggcgag atgacttggc
     1501 aagccaaaaa ctacacacag tattcgtcct actttatgaa gattacttgc ttgaaatagc
     1561 ttattggcaa atggcatata cagttattga accattctga tcttcggttg tatcgcacgc
     1621 agccgaccat gagttattgg ctgatgggaa tgacctggta agccatatgt aaccagttcg
     1681 tgaggtgcat attccgccgc cacctgtaac acgttttcca gttggacaag tagctgttgc
     1741 tccaggaaag cgccaacctg tggcctgtgc tgtagcaatt tgaagctcac cagatcctac
     1801 ctttctccag gtaccggatt ggcactaggc cagtgccaat ccggtcggtg gtcagggggt
     1861 aataaaatta attactcagc atgtaattgg tacaaatcat ccgtagcaat gaatcacttt
     1921 atcggcggta agagtggtgg ttcgatctat tacaagccga ttcaatgtcc gactggatac
     1981 attatgactg gaacacgcat gtacggtatt ggtgatggtg ttgatgagga acatgtcgat
     2041 gcctactgtt gcccctttaa ttaagctcca gcaccaggaa gattgaggca tgtgactgat
     2101 atggtcccac cagactcata atttccccaa gcataattac tcacatacca aacgttgttt
     2161 tggtcggaat atacctgttg aaaaccacca aagggtgagc cattcaggta agtccatgag
     2221 caatatgcat ggaccccgag ccttaaatta cgagtgtttt tagctatgtt gtaggtctgc
     2281 gttgtgaatt gaattttagg agcgccccag gtaccggatt ggcactggcc tacgtgccaa
     2341 tccggtacgt gg
//
LOCUS       DAFAVF010000414         1829 bp    DNA     linear   BCT 07-OCT-2021
DEFINITION  TPA_asm: Escherichia coli strain OT_ESBL_0359
            SAMN19016885-rid13104793.guided.320, whole genome shotgun sequence.
ACCESSION   DAFAVF010000414 DAFAVF010000000
VERSION     DAFAVF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19016885
            Sequence Read Archive: SRR14477458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-OCT-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001146116.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001146116.1
            Genome Coverage       :: 129x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/06/2021 09:50:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,582
            CDSs (with protein)               :: 4,582
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19016885-rid13104793.guided.320"
                     /strain="OT_ESBL_0359"
                     /isolation_source="Livestock"
                     /db_xref="taxon:562"
                     /country="Netherlands"
                     /collection_date="2011"
                     /collected_by="Mike Brouwer"
                     /note="genotype: ST58"
     gene            <1..314
                     /locus_tag="LGK42_004756"
     CDS             <1..314
                     /locus_tag="LGK42_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK2660875.1"
                     /translation="PNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSG
                     GGQIYNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKK
                     G"
     gene            445..1233
                     /locus_tag="LGK42_004757"
     CDS             445..1233
                     /locus_tag="LGK42_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBK2660876.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1435..>1829
                     /locus_tag="LGK42_004758"
     CDS             1435..>1829
                     /locus_tag="LGK42_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBK2660877.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIP"
BASE COUNT          429 a          472 c          479 g          449 t
ORIGIN      
        1 ttccaaatcg caaatatgca gtagtgtcaa agaacggaat ttcaagctca aatgaaaacg
       61 tcctagtttt tccttcaata gaaaatgctt tgaaagagct atcaaaagtt acagatcatg
      121 tatatgtctc tggcgggggt caaatctata atagccttat tgaaaaagca gatataattc
      181 atttgtctac tgttcacgtt gaagtcgaag gtgatatcaa attccctata atgcctgaga
      241 atttcaattt ggtttttgaa cagtttttta tgtctaatat aaattataca taccagattt
      301 ggaaaaaagg ctaacaatgc gttgcagcac cagtcgcttc gctccttgga cagcttttaa
      361 gtcgcgtctt tgtggttttg ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg
      421 ccgctgaact taacgttagg catcatgggt gaatttttcc ctgcacaagt tttcaagcag
      481 ctgtcccacg ctcgcgcggt gatcgagcgc catctggctg cgacactgga cacaatccac
      541 ctgttcggat ctgcgatcga tggagggctg aagccggaca gcgacataga cttgctcgtg
      601 accgtcagcg ccgcacctaa cgattcgctc cggcaggcgc taatgctcga tttgctgaaa
      661 gtctcatcac cgccaggcga tggcggaaca tggcgaccgc tggagctaac tgttgtcgct
      721 cgaagcgaag tagtgccttg gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg
      781 ctccgccacg acatcctttc cggaacgttc gagcctgccg ttctggatca cgatcttgcg
      841 attttgctga ccaaggcgag gcaacacagc cttgcgcttc taggcccatc cgcagccacg
      901 tttttcgagc cggtgccgaa ggagcatttc tccaaggcgc ttttcgacac tattgcccag
      961 tggaatgcag agtcggattg gaagggtgac gagcggaacg tcgttcttgc tcttgctcgc
     1021 atttggtaca gcgcttcaac tggtctcatt gctcctaagg acgttgctgc cgcatgggta
     1081 tcggagcgtt tgcctgccga gcatcggccc ctcatctgca aggcacgcgc ggcgtacctg
     1141 ggtagcgagg acgacgacct agcaatgcgc gtcgaagaga cggccgcgtt cgttcgatat
     1201 gccaaagcaa cgattgagag aatcttgcgt tgagcggcat gtgcgaaaag tgcatcgacc
     1261 cgcgccgagg gcatctgatg cctaactcgg cgttcaagcg gacgggctgc gcccgccgct
     1321 caactatgcg ttagatgcac taagcacata attgctcaca gccaaactat cggcactgtt
     1381 gcaaatagtc ggtggtgata aacttatcat ccccttttgc tgatggagct gcacatgaac
     1441 ccattcaaag gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc
     1501 aaatacggca tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc
     1561 gatcactcca cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg
     1621 cgctggtact ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg
     1681 aaggtcaatg gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc
     1741 gatttttatc tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc
     1801 ctcaacaacg tgaagaagtg gcagatccc
//
